LOCUS       DAORUK010000001        73140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_153_length_73140_cov_18.331272, whole genome shotgun sequence.
ACCESSION   DAORUK010000001 DAORUK010000000
VERSION     DAORUK010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 73140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 73140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73140
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153_length_73140_cov_18.331272"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PK818_00005"
     CDS             <1..335
                     /locus_tag="PK818_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HQY81149.1"
                     /translation="RQKEQFSDGVGYGWIDGLKAHAEANVSDADFAAAGQRFPINPPQ
                     TREAYFYRRIFEDIFPGDACANTVPGGKSIACSSPAAIAWDAAFATMADPSGRAVAGV
                     HNAAVAAI"
     gene            complement(332..985)
                     /locus_tag="PK818_00010"
     CDS             complement(332..985)
                     /locus_tag="PK818_00010"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HQY81150.1"
                     /translation="MQPDFWHQRWADNQIGFHQSAPTPLLLKHWPALGITPGAQVFVP
                     LAGKSLDMAWLASQGHRVLGVELSQLAVEQFFAEHDLQPGIHETKYGRHYVASGIELI
                     CGDAFALDADALRDCAAVFDRAALIALPPELRERYASELYASLPTGSRGLLVTLEYPQ
                     EERPGPPFSVTEDEVRALYSRDWQVDLLDRRPIPPEHPGYVGGVSRLDTATYALRKR"
     gene            complement(1089..3338)
                     /gene="parC"
                     /locus_tag="PK818_00015"
     CDS             complement(1089..3338)
                     /gene="parC"
                     /locus_tag="PK818_00015"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HQY81151.1"
                     /translation="MTDDSIRPTFHGFEQIPLREYAERAYLDYSMYVVLDRALPFIGD
                     GLKPVQRRIVYAMSELGLAAAAKPKKSARTVGDVIGKYHPHGDSACYEALVLMAQPFS
                     YRYPLIEGQGNFGSSDDPKSFAAMRYTESKLSPIAEVLLGELGHGTTDWTPNFDGTLE
                     EPTWLPARLPHLLLNGTTGIAVGMATDVPPHNLGEIVSACIRLLDDPDASVRDLCEHV
                     RGPDYPTAAEIITPAGDLLAMYETGTGSVRARGVFVKEAANIVVTELPYQVSPSKIIE
                     QIAAQMRAKKLPWLEDIRDESDHANPTRIVLVPRSNRVDAEQLMGHLFATTDLEKSYR
                     VNMNVIGLDGRPQVKNLKMLLSEWLLFRTDTVTRRLKHRLEKVERRLHLLEGLLVAFL
                     NLDEVIRIIRSEDEPKPALIARFALSVEQADYILDTKLRQLARLEEMKIRGEQDELDS
                     ERDRLISTLGSKARLKKLIKDELLADAKKFGDARRSPLVAREAAQALDETELVASEPM
                     TVVLSEKGWVRAAKGHDIDAASLSYRDGDSLLGAVKARSNLQVAFLDSAGRSYSSAVH
                     TLPSARGNGEPLTGRFTPAPGASFQALASGDLDARFVIASSHGYGFVTRFENLTSRNK
                     AGKALLSLSEGGRVLPPAAVADVAADRIVAVTSAGHVLAFALAELPELDKGKGNKIIE
                     IPKAKRGTERVVAIAVVPPSGKLLVASGARTMTLSFNDLDSYLGARGSRGGLLPRGWQ
                     KVDGLAVET"
     gene            3582..3734
                     /locus_tag="PK818_00020"
     CDS             3582..3734
                     /locus_tag="PK818_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81152.1"
                     /translation="MRGQRSTSRRRSALADALAAGWALVLLKLAVGIAILWALWPSLR
                     RLLVGP"
     gene            3734..4558
                     /locus_tag="PK818_00025"
     CDS             3734..4558
                     /locus_tag="PK818_00025"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HQY81153.1"
                     /translation="MPVPITTAGAAMRNVMRREVACIDRALGLQQGLDASVHEARKAI
                     RRLRALLGLCRGQFDGLDDIGGRLQAIGRGLSVLRDAQASIDTARKLAALQAATPWAA
                     AEDRLRQRRQRLVDAALAKDPGFARRRACIASIATTLDGLPWESLSAARLQRALRKSG
                     KRVARAGRRASKAPSARSLHRWRRRLRTLRMQLDVIAEVAPGSASRASATAAGRQGKA
                     LRRQADALGWRQDLEVLRRLLRPMRALPGKPGLLAGIREEIDRLGREMSKDVDQGA"
     gene            4640..5044
                     /locus_tag="PK818_00030"
     CDS             4640..5044
                     /locus_tag="PK818_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY81154.1"
                     /translation="MARVTGIGGVFFKSTGKGAALAAWYREHLGLALEDFGGAVLRWP
                     EDKAADGGLTVWCVADADSQWFSPSTASMMVNYRVDDLDGMLAQLRAAGVEAVAGPES
                     HENGKFAWIMDPEGNKVELWQPMPWDAANKDA"
     gene            complement(5061..5786)
                     /locus_tag="PK818_00035"
     CDS             complement(5061..5786)
                     /locus_tag="PK818_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQY81155.1"
                     /translation="MPVDGLVLLAAFASGLLGTVHCAAMCGGIASSLGGGQRGGLWDA
                     ALPNLGRVLGYALAGAIVGGLGGGLLGIVRIPGLGIAMRALVGVVLVIAGLRLLDRKA
                     RLPGFGGGAGSRLWQWLAPLRARAWPADTAGKRLAVGMLWGWMPCGLSSTLLAAAWLQ
                     ASALHGAATMAAFGLGTLPMMVPLTWAGLRVGQRLQRGGLRVAAGTLVIGAGLLTLAG
                     PWLMQVPVLHGVLEAIGCRSLPG"
     gene            complement(5776..5976)
                     /gene="ccoS"
                     /locus_tag="PK818_00040"
     CDS             complement(5776..5976)
                     /gene="ccoS"
                     /locus_tag="PK818_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HQY81156.1"
                     /translation="MNILLLLVPISLLLLGVAIWAFAWAVRRGQFDDLDTPAIDILRE
                     DDPLDPQRQRAPTASQAGDDAG"
     gene            complement(5973..8387)
                     /locus_tag="PK818_00045"
     CDS             complement(5973..8387)
                     /locus_tag="PK818_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQY81157.1"
                     /translation="MNAVAAGVGATLPAASGCFHCGEALPATPASLQLDGQLRRFCCN
                     GCAAAAQWIRDADLDDYYRLREQPGRRIDVDAAALAHWDREDVLAGHARAIDGGRELV
                     VVTDGMRCAACAWLIDRALAREEGVLEIAANAVTGRIRIAWDPARTPLSRPLRRLQAL
                     GYQPYLAAGDAQERGRRRERNRWLLRVGIAGLGSMQAMMFAEALYLDTANQMSLPMRD
                     FLRWITFLVATPVVFYAGWPFLAGAWRELRERAPGMDTLVAASTLLAYLASVAETVRG
                     GPHVWYDAAVMFVFLLLVARMLEQRVRTTANAQVDALARARPVLALRENADGGCETVP
                     LAQLAVGDIVRVAAGEAAPADGVLLDAGARFGEALLTGESQPQPKRAGDTVYAGSLCD
                     GRTARIRIHRTGADTRLAQLAALVERAQAQRPPAAQRADRIARAFVVAQLAGALVLYA
                     AWRVHAPERAFEVTLAWLVISCPCALSLAIPAAYAAASSTLARLGVLPVRAGGLERLA
                     AVDTVVFDKTGTLGDGAPQLDATRTFGTLDADAAQRIAAALERESTHPLAKAFAGVDG
                     AARAQAVTVHAGAGIEGVVDGMRWRLGHAAFAFGGTDDGALWLADGQRAAARFGIREH
                     LREDAAAALAALSGLGIDAQLLSGDGEAAVRRVADTLGIEKAFARQLPEQKLARLREL
                     QAGGHVVAMVGDGVNDAPVLAGADVSFAIGDGAALATRSADLVLASPALLRIPEAIAL
                     ARRSRTVVRQNLGWALGYNLLALPVAALGLVTPWLAALGMALSSLAVTLNALRLARTA
                     RIGGPR"
     gene            complement(8384..8899)
                     /locus_tag="PK818_00050"
     CDS             complement(8384..8899)
                     /locus_tag="PK818_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HQY81158.1"
                     /translation="MDAPRGAGGRPPWKEPMVWLVVAIPAASVLASVALLVTAARSSG
                     NNDVVADPVRRTAQIQVADLAPDIAARERGLVAVLRIDGERIELLPLAGVFPRDAALR
                     LDLQHPARARDDLSMLLQPATGGWQARQALPLEHDWTLTVGAIDRSWRLQGRLPRGQR
                     EVRLQPALVAP"
     gene            complement(8899..10440)
                     /locus_tag="PK818_00055"
     CDS             complement(8899..10440)
                     /locus_tag="PK818_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixG Ig-like domain-containing protein"
                     /protein_id="HQY81159.1"
                     /translation="MGNKQIPLEVLDAGGAAYVSERKVYPRDVSGPLNRLRVAAVFWL
                     LGMYYLFPWLSWDGRQAVLFDLPARKFHVFALTFWPQDFLFLAMLLMIAALALFFFTA
                     LAGRLWCGYACPQTVWTEVFLWMESAIEGDRHKRMKLDAGPWTRQKLLRKGGKHAVWL
                     LFALWTGFTFVGFFTPIRDLAARAWPFQWGGWEAFWVFFYALATWGNAGFLREQVCKY
                     MCPYARFQSAMFDRNTLIIAYDPMRGEPRGQRKRGLPGVLQRARGLLDPAAAYDYVFR
                     ASRHPSAAAQQAHAHGTITFDGGTEAAPLPKFAPEELGDCIDCTLCVQVCPTGIDIRN
                     GLQYECIACGACIDACDAVMDKMGYPKGLIRYATQNAIEGKPTRVLRPRILLYGTILL
                     GLVLAWGWGVANRAPLIAEVLRDRNALYRETSAGIENGYTLKIVNKTDQAQRYRIEFT
                     SDSKGLALREAPTVDVAAGSVASVPLTMVAPAGIRGRHPLRFEVSAIGGDASETVESS
                     FFGPL"
     gene            complement(10446..10742)
                     /locus_tag="PK818_00060"
     CDS             complement(10446..10742)
                     /locus_tag="PK818_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81160.1"
                     /translation="MADHTAPQFDHPPRPMAQRIGAILWPSFFAAGVATMVFFAFVDP
                     LALRDMTFPHIEISRGLGYSIGFFMFWMATAASSLFTWILLRPASRFNRPLPPH"
     gene            complement(10758..11690)
                     /gene="ccoP"
                     /locus_tag="PK818_00065"
     CDS             complement(10758..11690)
                     /gene="ccoP"
                     /locus_tag="PK818_00065"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HQY81161.1"
                     /translation="MTQGWSWYVIALVALNIAGCAWLLWWTARRRPGDPKPEDTSHVW
                     DGDITEYNKPLPRWWINGFYLTILFGIGYLAWYPGLGAFAGYGKWTSKGEHARDKATY
                     NAKLEATFAPYRGQPIDVLARDPAALKLGRSIFGNTCATCHGSAGQGAIGYPNLTDDI
                     WHWGGSPEQVLQTVLEGREGVMAPWGQVLAGMGGDNAVDNVVAYVRTLSSPEKGLQNN
                     YMAAQGKKHFDAVCVACHGVDAKGNQALGAPNLTDGYWLYGDSTAALRETIINGRHGV
                     MPAHGELLGETRARLVAAYAWSLSNGKAQAAATP"
     gene            complement(11687..11830)
                     /locus_tag="PK818_00070"
     CDS             complement(11687..11830)
                     /locus_tag="PK818_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HQY81162.1"
                     /translation="MVSGIVTAILLVVFIAGWAWAWSPRRKPAFDEAARLALSENPVE
                     DAR"
     gene            complement(11832..12455)
                     /gene="ccoO"
                     /locus_tag="PK818_00075"
     CDS             complement(11832..12455)
                     /gene="ccoO"
                     /locus_tag="PK818_00075"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HQY81163.1"
                     /translation="MSEQHNPHEKIEKNVGLLAVLIAVAVSFGGLAEIVPLMYQAEAI
                     QPLPGVKPYPALELAGRDVYVREGCYNCHSQMVRTLRFETERYGHYSLAGESVYDRPF
                     QWGSKRTGPDLARVGGRYSDDWHRVHLLDPRAVVPESNMPSFPWLAENALDGATVQQH
                     MRTLQRLGDPYTDAEVADAAADVQGKTELDAVVAYLQGLGKHAPKGG"
     gene            complement(12463..13902)
                     /gene="ccoN"
                     /locus_tag="PK818_00080"
     CDS             complement(12463..13902)
                     /gene="ccoN"
                     /locus_tag="PK818_00080"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HQY81164.1"
                     /translation="MQAITATYNDKVVRQFAVATVVWGIVGMLVGVIIAAQLYWPTLF
                     EGISWLGYGRLRPLHTNAVIFAFGGSALFATSLHVVQRTCHVRLLSDGLASFVFWGWQ
                     LVIVAAAITLPLGLSQGKEYAELEWPIDILITIVWVAYGWLFFGTIARRRVQHIYVAN
                     WFYGSFIIAVALLHIVNNAVLPASLTKSYSVYTGAVDAMAQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAGRPIYSYRLSIVHFWALIAVYMWAGPHHLQYTALPDWAQSVGMVFSL
                     ILLAPSWGGAINGVLTLSGVWHKLRTDPILKFLIVALSFYMIATFEGPMMSIKTVNSL
                     SHYTDWTVGHVHAGALGWVAMISIGSIYSMLPRLLGREQMHSVKLVDAHFWVHTLGVV
                     FYIASMWIAGVMQGLMWRATNDDGTLTYTFVEALNATYPYYLGRLFGGLLVLAGMLVM
                     AWNVLRTYQQAGAIVPQPLLPPDASEARA"
     gene            14032..14814
                     /locus_tag="PK818_00085"
     CDS             14032..14814
                     /locus_tag="PK818_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQY81165.1"
                     /translation="MAANDRAAIPLDLVRLRRSCAECSLQQLCLPAGIEGADLGRLEA
                     LVQRPRSLQPGDALFRLHDPLGSVYVTSEGAFKTIGINEAGEEHVLGFHLPGELFGLD
                     AIGSGRHRCSAIALTQARVCELPFATLATAATQLPSLQHQLLRVMGQSADLDHDHVDL
                     LSRRQASERIALFLQGMGERYRRIGRSGEDFQLPMSRDEIARYLGLALETVSRSFSRL
                     HEDGVLDVRGRRVRVLDAKRLHATASGCEADIARAAARPRRA"
     gene            14942..15757
                     /locus_tag="PK818_00090"
     CDS             14942..15757
                     /locus_tag="PK818_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQY81166.1"
                     /translation="MHDILVHAVRYRDRDPTASYAARLAASLRASLTAVFVPPAMPAI
                     PEYDVAAVIAEYAAWIERETRQARAAGPGFEAWSASLGVAHARWEVADGQVAAVLRHA
                     GRWHDLLVLGTGSEDAWQSEAGVSELVLATGLPCLVVPPAANDCALPHACVAVAWNGA
                     AEAIAALHAALPLLQSARRVVVLSGEAAPAAPGLVPFDIDAWARCHQVEVEYLAMDRD
                     EDVGAALLAAAGEVSADLLVAGAYGRSRLAEWALGGVTRHLIRHATIPLLLRH"
     gene            complement(15767..16540)
                     /locus_tag="PK818_00095"
     CDS             complement(15767..16540)
                     /locus_tag="PK818_00095"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQY81167.1"
                     /translation="MQRVNGKTCIVTGAALGIGRACALRLAGEGASVALFDMLDEEGH
                     ALLAELQAAGHEAGYWHVDVADEAAVRDAINAVAERFGGVHVLVNNAGIAGTNKPTHE
                     LTEAEWDRVQAVNVKGVFFCNKHAIPHMQRAGHGSIINLSSIYGLVGAADVPPYHASK
                     GAVRLMTKTDALYYAPDRIRVNSVHPGFIRTPMVEHHLRDSGAADVEAACREVGLAHP
                     LGHMGDPDDIAWGVVYLASDESKFVTGSELVIDGGYTAR"
     gene            complement(16626..19196)
                     /locus_tag="PK818_00100"
     CDS             complement(16626..19196)
                     /locus_tag="PK818_00100"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HQY81168.1"
                     /translation="MRPSSMQAAAESAPRAEAAPLRGLGSADAAARLLRDGPNALPQA
                     EHRSRWAIVLSVLREPMLLLLLAATAVYLVLGDAGEALLLAVSVLAVIGLTIYQEQKS
                     ERALEALRELGSPRARVLRDGSPRVLPAGALVVGDVILLAEGDRVPADARLLEDTDLH
                     VDESLLSGESVPVRRHIGGPAEAALVHASTLVVSGHGIAEVVATGARTEVGRIGVSLR
                     SLHVEPTPMQHEIRRVVVLFATLALATGIAVVALTLLARGDWTQAVLAGLTLAIATIP
                     EEFPMVLAVFLALGAWRMARHQALVRRTPAIEALGAITVLCTDKTGTLTRNRMAVAEL
                     VAGDRRAPPRPTMDPALQALLQTAVLASRADSIDPMDRALADAVAALGTHPIAPGPDA
                     SDLLREYPLTPACTAVSHAWRRGAEILVACKGAPETVAGLCHVEAGPRAALLAEVDAM
                     GRRGLRVLAVADARWHGDAALPPSMRDFDFGWRGLVAFADPLREGVVEAVAEAHAAGI
                     RVVMLTGDHPATARAIAAQAGIAAGTRGVVEGHPLEAMDDDALLRCATGSNVFARVRP
                     EQKLRLVAALKRDGQVVAMTGDGVNDAPALMSAHVGIAMGQRGTDVAREAAAIVLLDD
                     DFVTVVRAIRLGRTIYDNIRRAVHYILAVHVPIAGLALLPLVGGGPLVLLPLHVVFLE
                     LIIDPACSIVFERESEAAGADIMRRPPRSQEARLLGVRGLFANLLRGAAMFAAVAAVY
                     ALGLAQALPAAQVGALAFTALVTGNLGLIVVYRAGDSLWQTLRARNRAFAVVSALALA
                     VLLLVTRWPLAAGWFGFEPPPLAPWLLAVCLPLAVAALLKAGRREPAAPDAGVPATAG
                     "
     gene            complement(19228..20616)
                     /locus_tag="PK818_00105"
     CDS             complement(19228..20616)
                     /locus_tag="PK818_00105"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005413381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY81169.1"
                     /translation="MQDAHSKPSRFSLQFLGANATVTGSRHLVTAGNARILVDCGLFQ
                     GYKPLRLRNWAEFPVDPASIDAVLLTHAHLDHSGYLPRLVRSGFRGKVWCTEATRSLC
                     GILLPDSGRLLEEEAGHANHSGWSKHHPAEPLYSEADARASLARLRGVGFDHGFEPVP
                     GVHARFRAQGHILGAAAVTLEFEGRRITFSGDLGRPDDPLMPAPASLAASDWVVVEST
                     YGNRAHPADGLEEELLAVLQRVVARKGVVVIPAFAVGRAQMLLHVITQLQASGRLAKV
                     PVYLNSPMAASVTGLYRQFPRLHRLDAAELDAMQRTTHVVESVEQSKALNRHKGPMII
                     VSASGMATGGRVLHHLVAFAPDPRNAIVLCGFQAGGTRGAALAAGQKHLRIFGQEVQV
                     GAEVVQLAAGSAHADASEILAWLRTAPTPPRGVFVTHGEPDAADALRTRIKHELGWPA
                     HFPEYLEQVELA"
     gene            20883..21191
                     /locus_tag="PK818_00110"
     CDS             20883..21191
                     /locus_tag="PK818_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HQY81170.1"
                     /translation="METIRLSTIPAAAETLHPFDARGIGKRFRHAAIFGALDALSPGE
                     AMRFVNDHDPLPLLAQLQQRYGDALAIAYRQREPGAVVIDFTLRAGHGVGSTCCGSCG
                     "
     gene            complement(21289..21678)
                     /locus_tag="PK818_00115"
     CDS             complement(21289..21678)
                     /locus_tag="PK818_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HQY81171.1"
                     /translation="MIEFYPQIRLFHIFAVLLSGSIFALRGVAALAGARWPQARPLKW
                     LTYAIDTSLLTAALLLVAILPWSVFANGWLLAKIALLFAYVVLGVLALRPQRPRRVRA
                     LYFAAALATFALMYGIARAHHPLAWLA"
     gene            complement(21692..22918)
                     /locus_tag="PK818_00120"
     CDS             complement(21692..22918)
                     /locus_tag="PK818_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019336461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HQY81172.1"
                     /translation="MPTGTARPAPSPRLLAQAPHRLMFFIGASNLLLAMLWWTAWVIA
                     QRWQAWPMPQPTPYAGWLHAFVMQYQMLPSFMFGFLLTVFPRWMGLPDLERWRYLPVG
                     LGMFGGQLATLLGAFGWEAGMVVGALMTLAGWLAVIAHLMPLLWRERGTTWHARSCML
                     ALLLGLAGLLLWFAFLLNENALYAFASIKLGTFGVLLPVYLTVAHRMFPFFATNVVAG
                     YQPWRPLWLLAAFWPLLLAHVALELLHAYAWLWVVDLPLLGLALAMAWKLWPRGRMPG
                     LLAVLFIGLSWLPLTFALYAGQSAAYAMTGIHWLGRAPAHALFIGFFGSVLVAMVTRV
                     TQGHSGQPLVMPAAAWWAFAGVQVVAMVRVAAELADDPMGWMACAAIGWLLAFAPWVA
                     RLGRIYLSPRTDGKPG"
     gene            complement(22993..23754)
                     /locus_tag="PK818_00125"
     CDS             complement(22993..23754)
                     /locus_tag="PK818_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQY81173.1"
                     /translation="MGVLSNVPFPRQSPQTLADDGDSLRFCSTCAFSQACMDEGMDKG
                     ALMDLHVLVEHIGPFHAGDHLFREGEPFEAIAAVRAGTVKTYVVDRDGHEHVLGFHLP
                     GEVIGLNAIDGETYPCNAVALDTVMLCRFSFPKIAVLATRLPGLQRQLFRLLSRDIGR
                     AALLAGDWSADQRMAAFLVGLSRRLAARGFSATRFQLTMARTDIANYLRLAPETVSRV
                     LRRFQEEGRLVVDRRELELLDTAALEALAAPVLRA"
     gene            complement(23765..25165)
                     /gene="hemN"
                     /locus_tag="PK818_00130"
     CDS             complement(23765..25165)
                     /gene="hemN"
                     /locus_tag="PK818_00130"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HQY81174.1"
                     /translation="MESQAIAFNAELLRRYDRPGPRYTSYPTAPQFHAGFGARELRAV
                     ASASNGDPIPRRLSLYVHVPFCASPCFYCGCNRIITRDTARGEAYLARLYREIALTAP
                     LFDRDREVIQLHFGGGTPNFLSPAQLREVVDTLRSHFHFSDADERDLSIELDPRFVSP
                     ADIAELAAAGFNRASFGVQDFDPEVQVAVNRIQSVEETRAVVDACRGNGFRSVNIDLI
                     YGLPKQTREGFARTLDIVTGMRPDRVAVYGYAHLPELFKPQKQIVGADLPDAETRLAL
                     LQLAIERLTAAGYCYIGMDHFALPDDELALAQARGGLHRNFMGYTTHADSDLVGLGVS
                     AISHVGDSFSQNPRDLPSWQAALDEGRLPVFRGMRLDEDDQLRADLIQQLMCQGEIPM
                     AALERRYGIDFAAYFADALAKLQPLAEDGLVRIEADRITVTSRGRMLLRNIAMCFDRY
                     LDQPVTDARPRFSRAI"
     gene            complement(25269..25355)
                     /locus_tag="PK818_00135"
     tRNA            complement(25269..25355)
                     /locus_tag="PK818_00135"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25319..25321),aa:Leu,seq:cag)
     gene            25394..27982
                     /gene="rnr"
                     /locus_tag="PK818_00140"
     CDS             25394..27982
                     /gene="rnr"
                     /locus_tag="PK818_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HQY81175.1"
                     /translation="MSTDPRDADGRCTGAMYHRRMSKPPSKRGPGRGGKTPGKTGPTR
                     SEGTPTGRPGPKKAASKAPGWLPDAGLMRAFGGAGAGKPGARSGHPHADTAASRRAHP
                     PTARQPAMPASPARAGDPHAAREAQRYEQPIASREMILQVLAANDGPMDAEALAARLA
                     LTAPDRFEALGKRLGAMVRDGQLLQNRRGGFAPAAQMDLVPGVVIANPNGFGFLRPDA
                     GGGDDLFLPPFEMRKAMHGDRVLASVTGVDRSGRKEGAIVEVLERRLNRLLGRFTEED
                     GIAFVVPDDKRIQRNVMIPHDGRNEARAGQLVVVEITRAMDATRPPIGKVLAVLGDKL
                     TASLAVEAAIHGHEIPHEFPPAVLAEATAVPLQVDAATAARRVDLRALPLVTIDGEDA
                     KDFDDAVWCEPNRDGFRLIVAIADVSHYVRPGTPLDDEAQLRATSVYFPGFVVPMLPE
                     TLSNGICSLVPKTDRLCFVCDMQVGRDGIVTASEFYEAAMHSHARLTYTDVWNAVGEG
                     IPDDDRDAALAKIGGLLPQIQRLHQLYKVLDKARARRGAIEFESSEVRFVLDAKGAVS
                     QAGMLQRNDAHKLIEECMIAANVQAALYLMGVGIPAPYRNHDKPPESKYADLGEFLKE
                     FALRLPPWAKLRPKDFRALLEKIRERPDAALLESVILRSQSLAVYAPDNIGHFGLALE
                     AYAHFTSPIRRYPDLLVHRAIKHALAGGRRDAYEYSPHRMASLSLQCSERSRRADEAQ
                     REVDERYRAAWMEDHVGKEFEGTISGVTSFGLFVELDDSKVNGLVHVTQLANDFYHFD
                     AVRKTLTGERRGREYRLGDRVRVVVLKASVEDRKIDFRLVEEGVAAAKPLPERGKPAK
                     RQKQKY"
     gene            28135..28881
                     /gene="rlmB"
                     /locus_tag="PK818_00145"
     CDS             28135..28881
                     /gene="rlmB"
                     /locus_tag="PK818_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HQY81176.1"
                     /translation="MSKQAQWIVGLNAVASALEHDAEHVREVLLEAGGKNPRIVEIEQ
                     QARRKEIDVRRVAQGALDGVSGGVRHQGAAARYAAAKTWDEHELVGLVEAAGGKALVL
                     VLDGVQDPHNLGACLRSAAAAGATAVVFPKDKAVQVNATVRKTSAGAADTLPVFSVTN
                     LSRTLRDLQKAGVWIYGFAGEATASLYALDLKGNVALVMGSEGDGLRRLTRENCDQLV
                     KIPMPGEFESLNVSVATGIALFEAVRQRQP"
     gene            28900..29166
                     /locus_tag="PK818_00150"
     CDS             28900..29166
                     /locus_tag="PK818_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81177.1"
                     /translation="MALDLAWYDWVGLLGTAMILGGFALLQAGRLSGTGLVYQLLNLF
                     GAAGVLVSLLGTFNLSVFLLEAAWIAVSVYGIARSFKEKRTEAR"
     gene            complement(29225..29914)
                     /gene="rnt"
                     /locus_tag="PK818_00155"
     CDS             complement(29225..29914)
                     /gene="rnt"
                     /locus_tag="PK818_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008572178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HQY81178.1"
                     /translation="MTEIQETPAFKPMAERFRGYLPVVVDVETGGFDWNKHALLEIAA
                     VPIELDPHGRFVPGEVCHAHIEPAPGTSIDPKSLEVTGIKIGHPLRFAVDEREGLDKV
                     FVPVRAAAKKHGCQRAILVGHNAHFDLNFLNAAVNRVQHKRNPFHPFSVFDTVTMAGL
                     MYGQTVLARAIHAAGLDWDQREAHSAVYDAERTAQLFCIIANAWPRCGPSPGAMPEAS
                     AGTIGAMDGAD"
     gene            complement(29924..30667)
                     /locus_tag="PK818_00160"
     CDS             complement(29924..30667)
                     /locus_tag="PK818_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HQY81179.1"
                     /translation="MSTLREQLDAMARHVRDPAAHPGPPGIAARRLAIYRGLVYNNLD
                     ALLAGNFPVIRKILGDAGWQALVAGFLARHRCHTPLFTELGRELIAFLEAETDPRRPW
                     LAELAHYEWAELGLQLSDAVVPPHDPGGDLLAGVPMLSPLAWPLAYRWPVNRLAPEYQ
                     PGEPPPEPTLLLLRRGPDGRIHFSALSPLLFRLLELVDTDAGVDGRRLMRQLAMEAGH
                     ADFDGFLAEATPMLQRLRNEGVLLGTRID"
     gene            complement(30664..31488)
                     /locus_tag="PK818_00165"
     CDS             complement(30664..31488)
                     /locus_tag="PK818_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HQY81180.1"
                     /translation="MRQPLPAHSAGLGLRRALLDQLIEAPAGAFDFLECAPDNWIGVG
                     GVLGDKLATLASRHPLSGHGLSLSLGGPDPLDTTFLRQTRDFLARHQVVAYSEHLSYC
                     SASGHLYDLLPIPFTEAAVRHVAGRIAQVQDALGQRIAIENVSYYAAPFQAMGEIAFI
                     EAVLGEADCDLLLDVNNVYVNAINHGYDPRAFVDAMPSARIASIHVAGHYDEADDLKI
                     DTHGAAVKDAVWDLLGHAYQRHGVRPTLLERDFNLPPLPVLLAEVQRIRDLQARFR"
     gene            complement(31495..31935)
                     /locus_tag="PK818_00170"
     CDS             complement(31495..31935)
                     /locus_tag="PK818_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81181.1"
                     /translation="MSQSHKPIALVIGAAVATGTLVAGSLFTMQAMAGGYQQDPAKAE
                     AKAGEGNCGGMQPAAGAPAHTLASMDTDKDGRLSRAEFDAAHKGSEEKFAGHDGNGDG
                     FITQAEMDAHHAQMKDAKHDAASKGGEGKCGEGKCGEGKCGGNP"
     gene            complement(32050..32751)
                     /gene="phoU"
                     /locus_tag="PK818_00175"
     CDS             complement(32050..32751)
                     /gene="phoU"
                     /locus_tag="PK818_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HQY81182.1"
                     /translation="MNEHNHIVKSYDQEQQALLDELIRMGQMSVAQLEAALDVVERRD
                     DKAAERVIGNDEAIDALEQQVNHDVIRLIRRGPLAGDLRVILAALRVASDIERIGDLA
                     ANIAKRSMALNLTPPLPHTRGLDALGRLAARQVRDVLDAYVQRDGDAALRLRANDVEL
                     DTLYTGLFRELLTYMMEDARAITPCTHLLFMAKNLERIGDHATNIAENVWFLVHGEEL
                     MPPRDKRDESSTTVV"
     gene            complement(32751..33560)
                     /gene="pstB"
                     /locus_tag="PK818_00180"
     CDS             complement(32751..33560)
                     /gene="pstB"
                     /locus_tag="PK818_00180"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007966219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HQY81183.1"
                     /translation="MNDLHLAVAHPRDTPTLAPPVKISARNLDFWYDKFHAVKDIRLD
                     VPEKRVTALIGPSGCGKSTLLRVFNRIYALYPKLVAKGEVMLDGENILDPKYPMNRLR
                     SKVGMVFQKPVPFPMTIYENVAYGIRHHERLGKAEMDARVELALRQAALWDEVKDKLK
                     DSGLGLSGGQQQRLCIARAVALKPEVLLLDEPTSALDPISTSRIEQLVEELKQDFTIM
                     IVTHNMQQAARVSDYTAFMYLGDLIEHGATEQIFSNPSKQQTEDYITGRFG"
     gene            complement(33580..34434)
                     /gene="pstA"
                     /locus_tag="PK818_00185"
     CDS             complement(33580..34434)
                     /gene="pstA"
                     /locus_tag="PK818_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HQY81184.1"
                     /translation="MATPLYAIRAAKNIAAQVLAVLAAVFGIFWLVWIIWTTLRMGVS
                     SLNLALFTEMTPPPGAEGGGMLNAFFGSALMSMLGILLGAPIGVLAGTYLAEYSSKSW
                     LGETVRFVNDILLSAPSIVLGLFVYTMVVAQMGHFSALAGGIALAFIVLPVVVRTTDE
                     MLRLVPSQMREAALSLGVPQWKVTMQVLYRSALPGIVTGILLALARISGETAPLLFSA
                     LNNQYWTTDVLSPMANVPVVIFQYAMSPFENWHSLAWAGAFVLTLFVLLTSLVARAIV
                     SRNKMTHD"
     gene            complement(34434..35402)
                     /gene="pstC"
                     /locus_tag="PK818_00190"
     CDS             complement(34434..35402)
                     /gene="pstC"
                     /locus_tag="PK818_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008572163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HQY81185.1"
                     /translation="MNAIAPTAADDSRDLADIRTDNRFRWLVTGAGVFVLVSLAAAAL
                     SMLWGGRAAFQKFGIAFLWTDEWDAVQQQFGALVPIYGTLVTAFIAMLIAVPISFGIA
                     FFLTEIAPKWLRTPVGAAIELLAGIPSIIYGMWGLFVLVPVLAEHVYPWVDANFGQIP
                     FVGVLFAGPPLGLGMGTAGLVLAIMVIPFVSSVMREVFLTVPGRLKESAYALGATRWE
                     VVWDVVLPYTRSAVIGGIFLGLGRALGETMAVTFVLGNAHSLTMSLLEPGNSIAATIA
                     NEFTEADSEMYRSSLIALGFVLFIVTFIVLSIARLMLRSLNKREGN"
     gene            complement(35475..36575)
                     /gene="pstS"
                     /locus_tag="PK818_00195"
     CDS             complement(35475..36575)
                     /gene="pstS"
                     /locus_tag="PK818_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HQY81186.1"
                     /translation="MSRQLPRLAALSLCLLAGLAACKQEPAAAAASADPAAAPTAAPA
                     TAGDRKSVEITGAGATFIYPLLSKWSDDYNKATGARINYQSIGSGGGIAQIKAGTVDF
                     GSSDKPLAPEELAKDGLAQFPSAVGGVVPVVNLEGLEAGKLRLTGAVLADIFLGKVAT
                     WNDPAIAALNPGLPLPSSKINVVHRSDGSGTTFNFVNYLSKVSPAWKATVGEGTSVNW
                     PGGIGGKGNEGVAAYVKQIKGSIGYVELAYATENRMAYAGMQNAAGNWVQPSAASFAA
                     AAASADWKNARDFSLVVTDAPGADAWPITATNFILVYKQPKDAKRTRDTLDFFKWAFE
                     QGQAQADALHFVPLPAELVQQIEAYWASDIKA"
     gene            complement(36743..37726)
                     /gene="pstS"
                     /locus_tag="PK818_00200"
     CDS             complement(36743..37726)
                     /gene="pstS"
                     /locus_tag="PK818_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002804435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HQY81187.1"
                     /translation="MLAGGFVFTAAAADITGAGATFIYPLLSKWSADYSQATGNKINY
                     QSIGSGGGIAQIKAKTVDFGSSDKPLPPEELKAAGLGQFPSAIGGVVPVINIPGLKAG
                     AMKLDGELVADIFLGKVAKWNDPRIVALNGGVNLPDMKITVVHRSDGSGTTFNWVNYL
                     SKVSPEWKAKVGEGTSVKWPAGVGGKGNEGVAAYVKQIKGGIGYVELSYALQNKMAYS
                     RMKNAAGNYVNPSDASFAAAAASADWKNSKDFFLVMTNAPGDNSWPVAATNFILMHKK
                     PKDAARAKQALEFFRWAYANGDEQARSLDYVPLPDELVQQVEAYWAQNYKF"
     gene            38037..39359
                     /locus_tag="PK818_00205"
     CDS             38037..39359
                     /locus_tag="PK818_00205"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HQY81188.1"
                     /translation="MTMQRKTLALAVTMALAATSFQASAQDARDQELAALKQQLAELA
                     AKVQELEDRSDAQSDVNVDTATQLDTLANNSTKVETKGGIKVTSADKNFEASIGGRVH
                     FDAYAFDKDVAATTDTTEFRRARLTLSGKAYGWEYKVEEDFSGGSNLDGIRDLYIARN
                     MMGGKVTIGHFKPYRALEELTSSNEILMMERPSTTATGIYNGRQFQQGVGYLRAGATG
                     NYTFGVSAFNMRSAPTARNEGVGFAGRATWAPINDGTTAFHLGASYSFENLNKGTPTV
                     TAVSHYAGRRGPSQTMATTTAAVGTNDSQVSTYGLEAAYLSGPFFVQSEYARGNYGQA
                     SGGSQDVDAFYVQGSWMLNGGMKAYKPGTGVFGSPKVADKGLWELTARWDTMENNDLL
                     DTKTTSTVLGVNYYVNPNMRIMLNWTQGDNDKTGDEPSQLALRTQFAF"
     gene            complement(39473..40174)
                     /gene="nth"
                     /locus_tag="PK818_00210"
     CDS             complement(39473..40174)
                     /gene="nth"
                     /locus_tag="PK818_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQY81189.1"
                     /translation="MTVVAGRRRPLRGSRLTREEIADLFARLRALNPKPTTELEYASP
                     FELLVAVILSAQATDVGVNKATRKLFPRANTPAAILALGEDGLKRYIATIGLFNAKAA
                     NVIAMCRLLLDAHGGEVPRTRAGLEALPGVGRKTANVVLNTAFGEPAIAVDTHIFRVS
                     NRTGLAPGTNVRIVEDKLVKLVPAEFRLDAHHWLILHGRYTCTARKPQCPRCPVRDLC
                     RWPDKAASAGPVLLP"
     gene            complement(40171..40479)
                     /locus_tag="PK818_00215"
     CDS             complement(40171..40479)
                     /locus_tag="PK818_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014036553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81190.1"
                     /translation="MLAIDDLDAAMEHGLLDALPCPGCDAACNVRLIEARDARHSALA
                     ARERHRARAERLLRRAAGREAARRPVPASTAAPAPALPAAAADVLARALAKAARRGPR
                     "
     gene            complement(40542..41324)
                     /locus_tag="PK818_00220"
     CDS             complement(40542..41324)
                     /locus_tag="PK818_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009600779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQY81191.1"
                     /translation="MTDPLLLIDTADGVRTIRVNRPDKLNALNAATLDALDVAFADAA
                     ADAAVRVVVLTGSGPKAFVAGADIAEMNALTPVQGRDFSLRGTRMMRRVETLPKPVIA
                     MVNGFALGGGLELAMCCHLRIAADSAKVGQPEINLGLIPGFGGTQRLLRLCGRAATLE
                     LCLTGAPISAERALQLGIVNRVVPAADLEAETMRLATQLAAAAPLALRGMLDCVNIGG
                     ECGIGEGLEYESAQFGLVFSTDDMREGTAAFLEKRKPAFNGR"
     gene            41550..42284
                     /locus_tag="PK818_00225"
     CDS             41550..42284
                     /locus_tag="PK818_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HQY81192.1"
                     /translation="MKFRALAASVAALTLTAGIASAQTKPAAPAPAPAKPAAAPAKPA
                     APVVDKTHASYAFGWTLGQDLVNSGELVDIATVIRGLQDAYAKKQPAYTQEQLGAAYS
                     GFQQRVMQKREAEFRKLASDNEAQSAGFVTKFKDQTGVITLPSGIMYRVLRPGTGAKP
                     TATSQVEIVFQTFLAAVNVPLSGEQTPAPFKVADAPIPALKETLPLMQQGTVWEMVIP
                     PGKLGADNPQFAKQAVTIRAALSGVK"
     gene            42422..43239
                     /locus_tag="PK818_00230"
                     /pseudo
     CDS             42422..43239
                     /locus_tag="PK818_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510554.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
     gene            43239..44084
                     /locus_tag="PK818_00235"
     CDS             43239..44084
                     /locus_tag="PK818_00235"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HQY81193.1"
                     /translation="MAGWTTLVQAETLAVALNRPDLVIVDCRFSLLDPNAGEHAYGTT
                     HLPGAAYAHLERDLSDMSPGGEGRHPWPPADMFTARLARYGIEPGHQVVAYDDGDGAI
                     AARLWFLLKMLGHEKVAVLDGGWARWTALGLPVSSAPRAPAVSAYRAAYDHSRLFDAN
                     ALQAHLDNGGMLVDARAAARFRGDEEPLDRIAGHVRGAVNRPYALNLVDGRFKPPMQL
                     ADEYRALLGTHSPDQVVVMCGSGVTACHNLLAMERAGLRGGILYTGSWSGWISDPSRP
                     VATGG"
     gene            complement(44125..44895)
                     /locus_tag="PK818_00240"
     CDS             complement(44125..44895)
                     /locus_tag="PK818_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQY81194.1"
                     /translation="MSPNGSLVETLVHGHVTELRLARAPVNALNPDLCRAISGSLAGA
                     LANGARGIVLAGGPKVFSAGLDVPFLLSLGDDQAALLQAWTAFFDAARALASCPVPVV
                     AAMAGHAPAGGCVLALCCDYRIMADGPYRIGLNETQVGLVAPEGIQALMARAVGAHRA
                     ERLLVAGAMVESAQALRIGLVDEVTGIDDVATRARVWLQELLQLPQGPMLETRRIARA
                     DVAACLRDERIDLPRFVAGWMQPDTQAALRAMLARIGK"
     gene            44937..45365
                     /locus_tag="PK818_00245"
     CDS             44937..45365
                     /locus_tag="PK818_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HQY81195.1"
                     /translation="MKIILALLLSLLPLSACARDCAPQVKDGWIRLMPAGMPMHAGFG
                     RIENRCPMPASIVAASSPAYGSVELHESKLVDGISRMRQVPELRIAPDGAAVLQPGGL
                     HLMLMQPKATLKPGSRVAIEFELKDGRKLLGELEVRKPAP"
     gene            complement(45432..45836)
                     /locus_tag="PK818_00250"
     CDS             complement(45432..45836)
                     /locus_tag="PK818_00250"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQY81196.1"
                     /translation="MNNLFRRPVALRWSDLDAFNHVNNARYLTFIEQARIEWFDTIAE
                     PWVTTETAPVVASATLNFKRPIEYPASIFVELFTAKLGTSSVVIGHRIVDADGGLHCD
                     GSVVAVWIDRRSGRPTPLPDGVRRASEALLQG"
     gene            complement(45847..48858)
                     /gene="uvrA"
                     /locus_tag="PK818_00255"
     CDS             complement(45847..48858)
                     /gene="uvrA"
                     /locus_tag="PK818_00255"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQY81197.1"
                     /translation="MALDFIRIRGARTHNLKNIDLDLPREKLIVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSSYARQFLSVMDKPDVDHIEGLSPAISIEQKSTSHNPRSTVGT
                     ITEIHDYLRLLYARVGSPRCPDHHFPLEAQTVSQMVDQVLAMEAEPALAEQRWMLLAP
                     VVRERKGEHVQVFEQLRAQGYTRVRVNGHVHEIDAVPALGLRQKHTIEAVIDRFKPRA
                     EIRQRLAESFETALKLGDGMAIVQSMDAAQDSASVAAGRTPEATAAQDSASVAAGRTP
                     EATAADREPLLFSSKYACPVCDYSLPELEPRLFSFNSPVGACPTCDGLGVSQFFDPAR
                     VVAHPELSLAAGAMRGWDRRNAYYFSMITSLAKHYRFDVDAVWQTLPKQVRDIVLHGS
                     GSEAINLVYVGENGNKYQRKHSFEGILPNLERRYRETESAAVREELAKYISERPCTEC
                     GGARLNRAARNVFVADKPLPEVAMLPIDASLAFFEGLALPGWRGEIAARIVKEIRERL
                     GFLVDVGLDYLTLERKADSLSGGEAQRIRLASQIGAGLVGVMYVLDEPSIGLHQRDNE
                     RLLGTLTRLRDLGNTVIVVEHDEDAIRLADHIVDIGPGAGVHGGEVVAQGSLESVLQA
                     PRSLTGQYLSGRKAIGVPAHRHPPNRKLMLALKGACGNNLKDVDLEIPSGLFTAITGV
                     SGSGKSTLINDTLYALAANEINGASHTPAPYREAGGIDLFDKVVDIDQSPIGRTPRSN
                     PATYTGLFTPLRELFAQVPEARARGYAPGRFSFNVRGGRCEACQGDGLIKVEMHFLPD
                     VYVPCDVCAGKRYNRETLEILYKGYSIRDVLEMTVEDALELFQPVPSIARKLETLMDV
                     GLSYIKLGQSATTLSGGEAQRVKLSKELSRRDTGRTLYILDEPTTGLHFHDIEHLLAV
                     LHKLRDDGNTIVVIEHNLDVIKTADWVIDLGPEGGHRGGQIIATGTPEDVAANPASHT
                     GRFLAPLLERNRAGSKPAPKGAPRKARNKTT"
     gene            49118..49441
                     /gene="rplU"
                     /locus_tag="PK818_00260"
     CDS             49118..49441
                     /gene="rplU"
                     /locus_tag="PK818_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HQY81198.1"
                     /translation="MYAVLVTGGKQYRVMQGETLRVEKLEAEAGSEIKFDNILLLGDA
                     DGIKLGDALKGATVAAKIVGHGRADKVRIVKFRRRKHHRKQMGHRQHYTEIEITGIAG
                     SGDNK"
     gene            49454..49714
                     /gene="rpmA"
                     /locus_tag="PK818_00265"
     CDS             49454..49714
                     /gene="rpmA"
                     /locus_tag="PK818_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HQY81199.1"
                     /translation="MAHKKAGGSSRNGRDSNPKYLGVKIYGGQAIEAGNIIVRQRGTQ
                     FHPGMGVGLGRDHTLFALVDGKVEFATKGPKKRRMVSIVAAE"
     gene            49853..50935
                     /gene="obgE"
                     /locus_tag="PK818_00270"
     CDS             49853..50935
                     /gene="obgE"
                     /locus_tag="PK818_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HQY81200.1"
                     /translation="MKLVDEAEITATAGNGGNGCIAFRREKFIPLGGPNGGDGGDGGN
                     VWLQADENLNTLVDFRHETRFKAQRGENGMGSQMYGKAGEDRTILVPVGTVVHNVDTD
                     EVIGDLTAHGQRLLVAKGGKGGLGNMHFKSSVNRTPRRATPGTEGETRTLRLELKLLA
                     DVGLLGFPNAGKSTFVRAVSAATPKVADYPFTTLYPNLGVVSVEPGRSFVIADIPGLI
                     EGAADGAGLGSLFLRHVQRTRLLLHLVDIAPMDYEGSSLSPAGQVRAIENELRKYDPA
                     MLEKPRWLVLNKADLMFEDEAQAAAEAIVAELDWKAPWYITSAISREGTRPIMLAVQA
                     FFDRLREDELEAAANAAAGAGSDDAG"
     gene            complement(51078..51347)
                     /gene="rpsT"
                     /locus_tag="PK818_00275"
     CDS             complement(51078..51347)
                     /gene="rpsT"
                     /locus_tag="PK818_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HQY81201.1"
                     /translation="MANIKSAKKRAKQTVVRNERNVAQRSMLRTAVKKVIKALDANDA
                     EAATAAFVTAQPLLDRFAARGLIHKNKAARHKARLTARIKALKAA"
     gene            51503..53059
                     /gene="murJ"
                     /locus_tag="PK818_00280"
     CDS             51503..53059
                     /gene="murJ"
                     /locus_tag="PK818_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HQY81202.1"
                     /translation="MSGLLRSSAVFSAMTLLSRIAGFVRDMLQGMLFGTGGAMSAFIV
                     AYRIPNFLRRVFAEGSMAMAFVPVLNEIRQKGDKAALKDFIDHMAGALCAVVLVVAAA
                     GVLLAPVVAQLFAPGWSGEEAVKLGQTAAMLRVTFPYLVFISMMALAGSVLNSFGRFA
                     LPAFTPVLHNLTMIAAMFWLAPRFDIRPMGLAWGVLVAGFLQLALLWPALGRLGLRPR
                     LRMGFAHPDVRKVGKLMLPTLFSSSVAQVNLLVGTAFASLLVDGSQDWLYYSDRLIEF
                     PLGLFGVALGTVILPHLSRRHADADAAGYNASLDWGMRMALLVGVPAGLGLMLLAEPL
                     TATIYNYGKFTDHDTRMSAISLTVMALGIPGFMLSKVLAPAFYARQDTKTPMRAAIWT
                     VAVNVALTVAITWPLWRQGVAGAHGGIAAATGLAGLFNAWLLWRYLRRAGLHRPQPGW
                     GRYLLRMLLACLAMALAVLALRGWVGEWVVLADWRLRVGWLLLAVAAGAVTYVAALLA
                     MGMRPRDLRH"
     gene            53101..54054
                     /locus_tag="PK818_00285"
     CDS             53101..54054
                     /locus_tag="PK818_00285"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HQY81203.1"
                     /translation="MNRFFRDAVGGPLCPQGSVVCIGAFDGLHLGHRALVGRAVAHAR
                     ALGVPAVALGFEPLPREFFARGEHPPRLMLPRAKLEGLHALGCDSVGLLRFDAKLASM
                     PAQAFVRDVLVERLSAREVWVGPGFRFGNARGGDIALLQALGAEHGFVAHSIDPVHCS
                     DGERVSSTRIRAALVEGDFAHAAKLLGRPYAIAGHVVHGRQLGRTLGYPTANLRYGGK
                     APALRGIYASWVHGVTDAPWPSVSSFGTRPTVDGIEPLLEAHLFDFEGDLYGRRIEVE
                     FVARLRDEEKFPDLPSLVAQMHRDDAQARAILIQTQQRASA"
     gene            54129..56918
                     /gene="ileS"
                     /locus_tag="PK818_00290"
     CDS             54129..56918
                     /gene="ileS"
                     /locus_tag="PK818_00290"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HQY81204.1"
                     /translation="MRGDLPRREPETLARWEAEGLYARIRGVAKGRPQFVLHDGPPYA
                     NGAIHLGHAVNKILKDIIVKSKTLAGFDAPYIPGWDCHGLPIEIAIEKKFGKVGVKLD
                     AVAFRQKCREYASEQIDVQRRDFKRLGVLGDWENPYRTLDFKFEADEIRALARVVENG
                     HLLRGVKPVYWCFDCGSALAEAEIEYQDKVSPAVDVAYGARNPQALSRAFGSELPEGV
                     EVALPIWTTTPWTLPASLAISIGPDLDYVLVEGPLAANGARRWLVLAEALAEKALKRY
                     GVDEVVVLGRAVGEKLEGMLFAHPFYAERDIPVLLGDHVSAEDGTGAVHTAPGHGQED
                     FAVSQKYGLVEKYSAAQLNPVDGRGVYLPSTPPANGAELAGVHVWKANDVLVDVLRAS
                     GHLLAFSKLTHSYPHCWRHKTPVAFRATPQWFISMDQANLRDDALAAIKTVAWYPGWG
                     EARIAGMVDGRPDWTISRQRTWGVPIALFVHRESGEPHPRSVELMRAVADRVEREGVD
                     AWYALDAADLLGEEAADYDRITDILDVWFDSGVTHECVVRERGLGKPADLYLEGSDQH
                     RGWFQSSLLTGVAIDKAAPYRQCLTHGFTVDEHGRKMSKSLGNGIEPQEIMKTLGADI
                     LRLWIASTDYSNEMSLSQEILKRNADAYRRIRNTARFLLGNLHGFDPARDLVALDDMV
                     GLDRWIVHRAWQVQEEVAGAYARYDFAAVVQALMNFCSVDLGSLYLDVTKDRLYTMRQ
                     DSRGRRSAQTAMFQIAEAFVRWIAPVLSFTADEMWGQLPGERAGDVLFATWYDGLAPL
                     AGDVPLSATDMDHLLVLREQVAKVLEPMRAAGAIGAALDAEIELRCAVADQNWLSPLA
                     DELRFLLISGDVRVVPDADASGVIGVFAQPTAKPKCVRCWHRREDVGTDPRHPALCLR
                     CVSNIEGPGEARSWF"
     gene            56918..57421
                     /gene="lspA"
                     /locus_tag="PK818_00295"
     CDS             56918..57421
                     /gene="lspA"
                     /locus_tag="PK818_00295"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HQY81205.1"
                     /translation="MASAARANALPWLAASVVVIVLDQWSKAWVLAALPEYTAVPVID
                     GFWNWYRTYNTGAAFSFLADAGGWQKWFFMALASAICALMGWMLARTPRRDWRQALPL
                     ALVIGGAIGNVIDRVVHGHVVDFIQWHAGSFYWPAFNIADCAVVGGAIGIALSGLSGG
                     KPQRKAG"
     gene            57429..58382
                     /gene="ispH"
                     /locus_tag="PK818_00300"
                     /gene_synonym="lytB"
     CDS             57429..58382
                     /gene="ispH"
                     /locus_tag="PK818_00300"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HQY81206.1"
                     /translation="MDVLLANPRGFCAGVDRAIEIVKRAIETLGAPIYVRHEVVHNRF
                     VVDDLKARGAIFVEELAEVPDGNTVIFSAHGVSQAVRSEAARRGLKVFDATCPLVTKV
                     HLEVARQCRNGRDMVLIGHAGHPEVEGTMGQWNREGGRGSIHLVEDLDDAGTLRLEQP
                     GNCSYTTQTTLSVDDTRDIIAALQARFPAIQGPKNDDICYATQNRQDAVRELVGEGCE
                     AVLVVGSPNSSNSNRLRELAEREGAAAWLIDGADEIDPAWIAGRRRIGVTAGASAPDI
                     LVRGVLDRLRELGAASVRELHGEPEDMVFALPKELRLRLVD"
     gene            58414..58489
                     /locus_tag="PK818_00305"
     tRNA            58414..58489
                     /locus_tag="PK818_00305"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58447..58449,aa:Thr,seq:cgt)
     gene            58593..59996
                     /gene="radA"
                     /locus_tag="PK818_00310"
     CDS             58593..59996
                     /gene="radA"
                     /locus_tag="PK818_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HQY81207.1"
                     /translation="MAKPASNLSSQKRRTVHVCGECGADHAKWQGQCDACGAWNALSE
                     VVLETAAQAAAPASRRSGWAGKVDAPKVTALADVRTDAQARVSTGIGEFDRVLGGGLA
                     EGSVVLVGGDPGIGKSTLLLQALALMAGTLPGLYVTGEESLAQVAGRAQRLGLPLDGL
                     LALAETCVERILEHAVAARPRLVVADSIQTLWTESLAAAPGSVGQVRESAARLVRYAK
                     ETGTAVFLVGHVTKEGGIAGPRVLEHMVDAVLYFEGDSGSRFRVLRAFKNRFGAVNEL
                     GVFAMGDKGLREVPNPSAIFLSGASSAQPGSCVMVTREGTRPLLVEVQALVDASPLSN
                     PRRVAVGLEGNRLAMLLAVLHRHGGVVSGDQDVFVNVVGGIRVQETAADLPVLLAVLS
                     SLRDRPLAEKTIAFGEVGLSGEIRPVPNGEERLKEAATHGFRRAIVPRANAPKAGSYK
                     GMEVIAVERLADALEQA"
     gene            60129..62348
                     /locus_tag="PK818_00315"
     CDS             60129..62348
                     /locus_tag="PK818_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQY81208.1"
                     /translation="MVDQSARAGRQPGGAAPGFAHAGLTGSFLLDRDGRVLEADGAPV
                     SACCLADLVPEFDAAEFARWRIALDGFPAAVLRLDFPRVDAPAVSLRLEREIDGRYLA
                     TLGGVGDGDGDGYRERLENRLVFEHMIAALSTELIHAPSERLDGLIEQALGRIGSFFG
                     VDRAYLFRFSGDRASMDNTHEWVEEGISREAHKLQQVPMDHFGWLMRELLAGRFVHIP
                     VVADLPPEAATEAAEFEREGIRSLVLVPFGEAQAPEGFIGFDSVRRERHWSADILLGL
                     RLVGQMFYNALRAQVMSNSLITLASLDPLTGLGNRRLLGEQLAYALERSQREQTRLAV
                     VLVDLDDFKLVNDSYGHSLGDEILKTVASRLAASVPAADSIARLGGDEFVLVVHVDAL
                     DSLVQQVERLFEAMREPVELRGMRFVLRMSAGIALYPADATDAEALLRQADTAMYAAK
                     AGGRDRHAFFAPPMALASRTTLTLRHELQQALERDEIRPCYQPRVALSSSRVLGFEAL
                     ARWHHPERGVLLPGEFLEIAAQGGMLGRIDQRILAHALADLAGWRRSHPDCMVSVNLD
                     ASDLQDEALVAGLLQQLDTAGDLADAVEIEITETSLMRNIEQAARTLGRLREAAPGLR
                     IAIDDFGSGYSSLAYVGRLPVATLKIDRSFVAELGGGNPRNARAIIHSIVDLSRSLGL
                     HVIAEGVETIGQADELRRLDCLEAQGFLFAPGLPHAQASALLQAGFVTAAGVHAVRS"
     gene            complement(62345..63133)
                     /gene="ccsA"
                     /locus_tag="PK818_00320"
     CDS             complement(62345..63133)
                     /gene="ccsA"
                     /locus_tag="PK818_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQY81209.1"
                     /translation="MTIALIAIALYLAATGWLVASVQRAEGVPRGWLLPANMALLLHG
                     ATHYMAWRDTGITDLDFFAALSLAGLGMAILTAVVGASGRMRALGVVVFPLAALGLLG
                     YALHGPARQPEPLDWRLLLHAWLALLAYATLAIAAVLAVFLWLQERALRRREFHRWLR
                     ALPPLTELETLLFRTIMAGFVLLGAVLLTGVLFVDDLLAQHLVHKTVLSVMSWLAFGA
                     LLVGRWRLGWRGATAVRWTLVAMALLLLAFFGSKFVLELVLARG"
     gene            63212..64582
                     /gene="ffh"
                     /locus_tag="PK818_00325"
     CDS             63212..64582
                     /gene="ffh"
                     /locus_tag="PK818_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HQY81210.1"
                     /translation="MFESLTQRLSGTIERLRGRGRLTESNITEAVREVRIALLEADVA
                     LPVVQALVQRIKVRAVGQEVMRSLTPGQVLVRIVRDELTAVMGSQASDLNLNVPAPAV
                     ILMAGLQGAGKTTTVAKLAKHLKEKRKKKVMVVSADVYRPAAIEQLQTLAGQVGAVFF
                     PSDAAQKPEAIVKAAIDEAKKTFADVLIVDTAGRTSIDDAMMAEIKALHGAVNPVETL
                     FVVDSMTGQDAAVTAKHFGEALPLTGVVLTKTDGDARGGAALSVRYITGKPIKFVGVG
                     EKPDGLDVFHPDRAAGRILDMGDVLSLVEQVEQQVDHDKAKKLAEKVAKGKKFDLNDM
                     RDQLEQMQNMGGLHGLMDKLPGVGALPEAVKQQVTGKEVPRMIAVINSMTKKERRNPD
                     LLNGSRRARVARGSGLTPADVNKVLKQYQQMEKMMGKLGRGGMKGMMRGLSGMMGGRG
                     SMPPMR"
     gene            64662..64910
                     /gene="rpsP"
                     /locus_tag="PK818_00330"
     CDS             64662..64910
                     /gene="rpsP"
                     /locus_tag="PK818_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HQY81211.1"
                     /translation="MVKIRLTRGGAKKRPFYHIIVTDSRSARDGRNIERVGFYNPVAQ
                     GQEKRIELDLERVKHWVDNGAQLTDKVADLYKQAAKAA"
     gene            64946..65467
                     /gene="rimM"
                     /locus_tag="PK818_00335"
     CDS             64946..65467
                     /gene="rimM"
                     /locus_tag="PK818_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011407909.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HQY81212.1"
                     /translation="MDAPDGRQRNITVGRLHGAFGVRGEVKLESFTDPLRAIASYQPW
                     ILRDARGVERACEGVRVREGVKGLIASMPGIEDRDAADALRGVEVLVPRSALPPPAPG
                     EYYWVDLEGLRVVNAEGVDFGLVSHLFSTGANDVLVASGERERMIPFVQPDFVRGVDF
                     DAGVVTVDWDPDF"
     gene            65479..66249
                     /gene="trmD"
                     /locus_tag="PK818_00340"
     CDS             65479..66249
                     /gene="trmD"
                     /locus_tag="PK818_00340"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HQY81213.1"
                     /translation="MRFDIVSLFPGFVAQLAAHGVVGRAGERGLLSINGWNPRDFAEG
                     NYRRVDDRPCGGGPGMVMMVDPLRAAIHAARAADPAPARVVYLSPQGTRLTQAKVREL
                     AALPRLVLLCGRYEGVDERLLQAEVDEELSIGDYVLSGGELAAAVLVDAVARLQDGAL
                     NDAESALQDSFEGDGLLDCPHYTRPVEHELGSVPEVLLSGNHAGIARWRRQQALGRTW
                     LRRPDLLDEAGLSKADRTLLDAFRAQYAAAPRDAGEGG"
     gene            66377..66793
                     /gene="rplS"
                     /locus_tag="PK818_00345"
     CDS             66377..66793
                     /gene="rplS"
                     /locus_tag="PK818_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HQY81214.1"
                     /translation="MNKPSIHTLLQEFETAQVTRKLPDFGPGDTVVVNVKVKEGNRER
                     VQAYEGVVIGKKNAGLNSAFTVRKISHGFGVERVFQTHSAAIDSVQVKRRGDVRASKL
                     YYLRGLEGKKARIKEDLAGNAKAKAAALAAAAAAAE"
     gene            66875..67282
                     /locus_tag="PK818_00350"
     CDS             66875..67282
                     /locus_tag="PK818_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HQY81215.1"
                     /translation="MQTETQPAAGVRLDLWLWAARFYRTRALAKHAIETGKVDVAGQR
                     AKPSRSVRIGDALVVARGEERFEIEVLALSDMRGPASVAQALYRESAASRLAREQARA
                     MRAAAHNGFRPPETRPDKRARRLILALGDLDAL"
     gene            67429..68169
                     /locus_tag="PK818_00355"
     CDS             67429..68169
                     /locus_tag="PK818_00355"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HQY81216.1"
                     /translation="MTVTVLKLGSRGEPVRRLQDALNRKLPGAVLTVDGDFGNRTLAA
                     VRRFQDEAWLVVDGEAGQCTQNALFDAEAYAPVRHVRPFIPQPTNSTCWAAATAMLKR
                     STVAAVKAATPADMWDDTNGLYNQSDVGDWVPQTQRYARAHGLLYYPPSSWIPRAFAD
                     LVRQGPVMVDTLWDTRGYLTRVPSGYLGSPGHMTLVVGVRGDNDPTGRGTSLMVYDPW
                     EPNVGKRWRVSYQRWVGEVSTRTYRLFQ"
     gene            complement(68227..70407)
                     /gene="katG"
                     /locus_tag="PK818_00360"
     CDS             complement(68227..70407)
                     /gene="katG"
                     /locus_tag="PK818_00360"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HQY81217.1"
                     /translation="MPTESKCPFHGNTAGTTNRDWWPNQLRLDLLNQHSPKSDPMGAG
                     FDYAKEFATLDYVALKADLAALMTDSQDWWPADFGHYGGLMVRLAWHSAGTYRIGDGR
                     GGGGRGQQRFAPLNSWPDNGNLDKARRLLWPIKQKYGRAISWADLMLLAGNVAMETMG
                     FKTFGFACGREDTWEPDMDVYWGRETRWLGGEERHAGNDAGARSLENPLAAVQMGLIY
                     VNPEGPEGNPDPLAAARDIRETFARMAMDDEETVALIAGGHSFGKAHGAGPASNVGVE
                     PEAGSLEAQGFGWQNGFGAGTGADTITSGLEVTWTKTPAQWSNDYFEHLFGFEWELSK
                     SPAGAHQWVAKGAEPSIPHAYDASRKLVPTMLTTDLSLRFDPAYEKISRRFLENPDQF
                     ADAFARAWFKLTHRDMGPRARYLGPEVPAEVLAWQDPIPAVEHPLVDAGDIAALKRDI
                     LASGLSVSELVSTAWASASTFRGGDKRGGANGARIRLAPQKDWAANDPAQLARVLAAL
                     EAIQASFNGSAAGGKRVSLADLIVVGGNAAIEQAAKDAGLDITVPFSPGRADATQEQT
                     DVDSFAPLEPFADGFRNYLARESAVPAEHLLVDKAQLLTLTAPEMTVLVGGLRVLGAN
                     AGGSKDGVLTDRPGTLSNDFFVHLLDMGTAWTPAGGNRFEGRDRTTGTMKWTASRVDL
                     AFGSNSVLRALAEVYASADGKQKFAEDFAAAWAKVMELDRFDLR"
     gene            complement(70649..>73140)
                     /gene="mutS"
                     /locus_tag="PK818_00365"
     CDS             complement(70649..>73140)
                     /gene="mutS"
                     /locus_tag="PK818_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQY81218.1"
                     /translation="DFYELFYDDARKAARLLDITLTQRGNSAGQPIPMAGVPHHAAEG
                     YLARLVALGESVAICEQIGDPAASKGIVERKVVRIVTPGTVTDEALLDERRDTLLLAV
                     SRGGMSSKGRGYGLAWADLASGRLRANEVAGEDALEAELARLQPAETLVPDEDGWPAC
                     VLDSRGLRRRAPWLFDADSGRRQLLQFFAVHDLTAFGLEDRPLAIAAAAAVLGYVEET
                     QKQRLPHLRSIALETSDDAIAMNAATRRHLELDSRVDGDVKATLLGVLDTTVSPMGGR
                     LLRRWLHRPLRDRRVVGERHHAVQTLIDHRTDEDLREAFRSLGDMERILSRVALRSAR
                     PRDLSTLRDGLSLLPAVRDILAPLDSPRLAALHAELGEHDATARMLRAAIVTQPPLLA
                     RDGGVVAEGFDAELDELRQLSTNADQFLIDLEAREREATGIATLKVGYNRVHGYYIEI
                     SKGQSDRAPVHYTRRQTLTGAERYITEELKGFEDKVLSARERALAREKLLYDGVLDAL
                     NAVLEPLRRCALALSELDVLAGFAERAQALDWSRPALRDAPGIHIERGRHPVVEALRD
                     APFEPNDLVFDEATRMYVITGPNMGGKSTFMRQNALIVLLACIGSFVPATKAEIGPID
                     RILTRIGAGDDLARGQSTFMVEMSETAYILHHASEHSLVLMDEIGRGTSTYDGLALAD
                     AVARQLASANRSYTLFATHYFELTALAEPGSGIANLHLDAVEHGDALVFMHAVRPGPA
                     NRSFGLQVAALAGLPKAALQQARTRLAELEAQSRDTPHVPLSAPALDQPQQFGLFAPS
                     SAALEALAGIDPDDLTPKQALEALYRLKSLG"
BASE COUNT        11088 a        25006 c        26054 g        10992 t
ORIGIN      
        1 ggcggcagaa ggagcagttc agcgatggcg ttggctacgg ctggatcgac ggcctgaagg
       61 cgcatgccga agcgaacgtg agcgacgcgg atttcgccgc ggccgggcaa cgcttcccga
      121 tcaacccgcc gcagacccgc gaggcgtact tctatcgccg gatcttcgag gacatcttcc
      181 cgggcgacgc ctgcgcgaac acggtgccgg gcggcaagtc gatcgcgtgc tcgtcgccag
      241 cggcgatcgc gtgggatgcc gcgttcgcga ccatggcgga tccttccggc cgcgcggtgg
      301 ccggcgtgca caacgccgcg gtggcggcga tctagcgctt gcgcagggcg taggtggcgg
      361 tgtccagtcg ggacacgccg cccacgtaac ccgggtgttc cggcgggatc ggcctgcggt
      421 cgagcaggtc gacctgccag tcgcgcgaat acagcgcgcg aacttcatcc tccgtgaccg
      481 agaacggcgg tccggggcgt tcttcctgcg ggtattccag ggtcaccagc agcccgcggg
      541 acccagtggg cagggatgca tacagttcgc tcgcatagcg ttcacgcaac tccggcggca
      601 gcgcgatcag ggcggcgcgg tcgaacaccg ccgcgcaatc gcgcagggca tcggcgtcga
      661 gcgcgaaggc gtcgccgcag atgagttcga tgccgcttgc aacgtagtgg cggccgtatt
      721 tcgtttcgtg gatgccgggc tgcaggtcgt gctcggcgaa gaattgttcg acggccagtt
      781 gcgacagttc cacgcccagc acgcgatggc cttgcgatgc gagccaggcc atgtccagcg
      841 acttgccggc cagcggcacg aagacttgcg cgcccggcgt gatgcccagc gccggccagt
      901 gcttcaacag cagcggggtg ggcgcggact ggtggaagcc gatctggtta tcggcccagc
      961 gctggtgcca gaaatcgggt tgcatgcgtg tctcctcgcg cgacggcgcc tgccgccgcg
     1021 catttcgcca cgcggctcga aatgccgcta cgggaaacgc ccgacggtct cgccgtcgat
     1081 gtgggtggtt acgtctcgac ggccaggcca tccaccttct gccatccgcg cggcagcagg
     1141 ccgccacgcg aaccgcgggc gccgaggtag ctatcgaggt cgttgaagga cagggtcatg
     1201 gtgcgcgcgc cgctggcgac cagcagtttg ccggacggcg gcaccaccgc gatcgcgacc
     1261 acgcgctcgg tgccgcgctt ggccttcggg atttcgatga tcttgttgcc cttgcccttg
     1321 tcgagctccg gcagttccgc cagcgcgaaa gcgagcacgt ggccggcgct ggtgacggcg
     1381 acgatgcggt ccgccgcaac gtcggcgacc gcggccggcg gcagcacgcg gccgccctcg
     1441 gacagcgaca gcagcgcctt gccggccttg ttgcggctgg tgaggttctc gaagcgggtg
     1501 acgaagccgt aaccgtggct ggaggcgatc acgaagcggg catcaaggtc gccgctggcc
     1561 agcgcctgga agctcgcgcc cggcgcgggc gtgaagcggc cggtcagcgg ctcgccattg
     1621 ccgcgcgcgg agggcagggt atgcaccgcg ctcgaatagc tgcggccggc cgaatccagg
     1681 aatgccacct gcaggttgct gcgcgccttg accgcaccca gcaggctgtc gccatcgcga
     1741 taggacagcg atgcggcgtc gatgtcgtgg cccttggcgg cgcgcaccca gcccttctcg
     1801 ctgagcacca cggtcatcgg ctcgctggcg accagttcgg tttcgtcgag cgcctgcgcg
     1861 gcttcgcgcg cgaccagcgg actgcgccgc gcatcgccga acttcttcgc atccgccagc
     1921 aattcgtcct tgatcagctt cttcagcctg gccttgctgc ccagggtgga gatcaggcgg
     1981 tcgcgctcgc tgtcgagttc gtcctgctcg ccgcggatct tcatctcctc cagccgcgcg
     2041 agttgccgca gcttggtgtc gaggatgtag tccgcctgct ccacggacaa cgcgaagcgc
     2101 gcgatcagcg ccggcttcgg ttcgtcctcg ctgcggatga tgcggatcac ctcgtccagg
     2161 ttgaggaacg ccaccagcag gccttccagc aggtgcaggc ggcgctcgac cttctcgagg
     2221 cggtgcttca ggcgacgggt cacggtgtcg gtgcggaaca gcagccactc gctgagcagc
     2281 atcttcaggt tcttgacctg cgggcggccg tccagcccga tcacgttcat gttgacccgg
     2341 tagctcttct cgaggtcggt ggtcgcgaac aggtggccca tcaattgctc ggcatcgacc
     2401 cggttgctgc gcggcaccag cacgatgcgg gtcgggttgg cgtggtcgga ctcgtcgcgg
     2461 atgtcctcca gccacggcag tttcttcgcg cgcatctgcg cggcgatctg ctcgatgatc
     2521 ttcgacggcg acacctggta gggcagctcg gtgaccacga tgttcgcggc ttccttcacg
     2581 aacacgccgc gcgcacgcac gctgccggtg ccggtttcgt acatcgccag caggtcgccg
     2641 gccggggtga tgatctcggc ggcggtggga tagtccggcc cgcgcacgtg ctcgcacagg
     2701 tcgcgcacgc tggcgtccgg gtcgtccagc aggcggatgc aggcgctgac gatttcgccc
     2761 aggttgtgcg gcggcacgtc ggtggccatg cccacggcaa tgccggtggt gccgttgagc
     2821 agcaggtgcg gcagccgcgc cggcagccag gtcggctcct ccagggtgcc gtcgaaattc
     2881 ggtgtccagt cggtggtgcc gtggcccagt tcacccagca acacttcggc gatcggcgac
     2941 agcttggatt cggtgtagcg catcgccgcg aacgatttcg ggtcgtcgct ggagccgaag
     3001 ttgccctggc cctcgatcag cggatagcgg tacgagaacg gctgcgccat cagcaccagc
     3061 gcttcgtagc aggccgagtc gccgtgcggg tggtacttgc cgatcacgtc acccacggtg
     3121 cgggcggatt tcttcggctt ggcggcggcg gccaggccca gctcgctcat cgcgtagacg
     3181 atccggcgct ggaccggctt gaggccgtcg ccgatgaacg gcagcgcgcg gtcgagcacc
     3241 acgtacatcg aatagtccag gtaggcgcgc tcggcgtact cgcgcagcgg gatctgttcg
     3301 aagccgtgga aggtggggcg gatgctgtcg tcggtcatgc ctccattcta cggactggct
     3361 ggcggccggt ccccgggggt cgggaaacac ctacccaggc ccggggactt ccccgactgc
     3421 gtattcgctg caggatccgc acactgtgct ggctggcaac gtgcaacgcc gacgggtcat
     3481 ccacgtgcac gggggccgca tggatagcgg ctctgccagc accccctcgg gcggccgggc
     3541 ggcaggctat cctgcatcct cgccggggat gcgggggggc catgcgggga caacgctcga
     3601 catcgcgtcg caggtccgcc cttgccgatg cgctggcggc gggctgggcg ctggtgttgc
     3661 tgaagctggc ggtgggcatc gcgatcctgt gggcgctgtg gccgtcgctg cggcgacttc
     3721 tggttggccc ctgatgcccg taccgatcac cactgccggt gcagcaatgc gcaacgtgat
     3781 gcgccgggaa gtcgcgtgca tcgatcgcgc acttggcctg cagcagggcc tggatgcctc
     3841 ggtgcacgag gcacgcaagg cgatccgccg cctgcgcgcg ctgctggggc tctgccgtgg
     3901 ccagttcgac ggcctcgatg acatcggtgg tcgcctgcag gccatcgggc gcgggctgtc
     3961 ggtgctgcgc gatgcgcagg ccagcatcga taccgcgcgc aagctggcgg cgctgcaggc
     4021 cgccacgccg tgggcggcgg cggaggaccg gctgcggcag cgcaggcagc ggctggtgga
     4081 cgcggcgctg gcgaaggatc cgggattcgc gcgtcggcgc gcctgcatcg ccagcatcgc
     4141 caccacgctg gacggcttgc cgtgggagtc gctgtccgcg gcccgcctgc agcgtgcgct
     4201 caggaaaagc ggcaagcgcg tggccagggc cggacgccgt gccagcaagg ccccgtcggc
     4261 gcgcagcctg catcgctggc ggcgccggct gcgcacgctg aggatgcaac tggacgtgat
     4321 cgccgaggtc gcgcccgggt cggcatcgcg cgcatctgcc accgccgccg gtcgccaggg
     4381 caaggccctg cgccggcagg cggatgcgct gggctggcgc caggacctgg aggtcctgcg
     4441 ccggctgctg cggccgatgc gcgcgttgcc gggcaagccg ggcctgctgg ccgggatccg
     4501 cgaggagatc gatcgcctcg ggcgggaaat gtcgaaggat gtggaccaag gcgcgtgatc
     4561 ggcgggtcat tgatgtccgt gccggcgccg tcggcatggc ccagaatgcc ccctggacca
     4621 tttcgagcag gaggcaccaa tggcaagggt caccgggatt ggcggcgtgt tcttcaagag
     4681 cacgggcaag ggtgcggcgc tggcggcgtg gtaccgggaa cacctggggc tggcgctcga
     4741 ggacttcggc ggtgcggtgc tgcgctggcc ggaggacaag gccgcggatg gcggcctgac
     4801 cgtgtggtgc gtcgccgatg ccgacagcca gtggttcagc cccagcaccg cttcgatgat
     4861 ggtcaactac cgcgtcgacg atctcgacgg catgctggcg cagttgcgcg ccgccggggt
     4921 ggaagcggtt gccggccccg agtcgcacga gaacggcaag ttcgcctgga tcatggatcc
     4981 cgagggcaac aaggtcgagc tgtggcagcc gatgccctgg gatgcggcca acaaggacgc
     5041 ttgagctgcg cgacccatcg tcagcctggc aggctgcggc agccgattgc ctccagcacg
     5101 ccgtgcagca ccggaacctg catcagccac ggcccggcga gggtcagcag gccggcgccg
     5161 atcaccagcg tgccggcggc gacgcgcagg ccgccgcgct gcaggcgctg gccgacccgc
     5221 agcccggccc aggtcagcgg caccatcatc ggcagggtgc ccaggccgaa cgcggccatc
     5281 gttgccgcgc cgtgcagggc gctggcctgc agccacgcgg cggcgagcag ggtgctgctc
     5341 aacccgcacg gcatccagcc ccacagcatg cccaccgcca gccgcttgcc ggcggtatcc
     5401 gccggccacg cgcgcgcgcg caacggcgcc agccattgcc acaggcgcga tccggcgccg
     5461 ccgccgaagc ccggcaggcg cgccttgcga tccagcaggc gcagcccggc gatcaccagc
     5521 accaccccga ccagggcccg cattgcgatc ccgaggcccg ggatgcgcac gatgcccagc
     5581 agtccgccgc cgagcccgcc gacgatcgcg ccggcgagcg cgtagccaag cacgcggcca
     5641 aggttgggca gcgctgcgtc ccacagcccg ccgcgttggc cgccgccgag gctgctggcg
     5701 atcccgccgc acatcgccgc gcaatgcacg gtgccgagca ggccactggc gaacgcggcc
     5761 agcagcacca ggccgtcaac cggcatcgtc gccggcctgg ctggcggtag gtgcgcgctg
     5821 ccgctgcgga tcgagcggat cgtcctcgcg caggatgtcg atcgcggggg tgtcgaggtc
     5881 gtcgaactgg ccgcgcctga ccgcccacgc gaacgcccag atcgcgaccc cgagcagcag
     5941 caggctgatc gggaccaaca gcagcaggat gttcatcgcg ggccgccgat gcgcgcggtg
     6001 cgcgccagcc gcaatgcgtt gagggtcacg gccagcgagg acagcgccat ccccagcgcc
     6061 gccagccatg gggtgaccag gccgagcgcg gccaccggca gcgccagcag gttgtagccc
     6121 agcgcccagc ccaggttctg gcgcaccacc gtgcgcgaac gccgggccag cgcgatcgcc
     6181 tccgggatgc gcagcagcgc gggcgaggcc agcaccaggt cggcgctgcg ggtggccagc
     6241 gcggcgccgt cgccgatcgc gaacgacacg tccgcgccgg ccagcaccgg tgcgtcgttg
     6301 acgccgtcgc cgaccatcgc caccacgtgc ccgccggcct gcagttcgcg cagacgcgcg
     6361 agcttctgct ccggcagctg ccgcgcaaag gccttttcga tgccaagggt gtcggcgacc
     6421 cggcgcaccg ccgcctcgcc gtcgccgctg agcagctgcg cgtcgatacc cagcccggac
     6481 agcgccgcca gcgccgccgc tgcatcctcg cgcaggtgtt cgcggatgcc gaaacgcgcg
     6541 gccgcacgct ggccgtcggc cagccacagc gcgccgtcgt cggtgccacc gaaggcgaac
     6601 gcggcatgcc caaggcgcca tcgcatgcca tcgaccacgc cctcgatgcc ggcgccggcg
     6661 tgcacggtca ctgcctgcgc gcgcgccgcg ccgtcgacac cggcaaaggc cttcgccagc
     6721 ggatgggtgc tctcgcgctc cagcgcggcg gcgatgcgtt gcgcggcgtc ggcatccagt
     6781 gtcccgaagg tgcgggtggc atccagctgc ggcgcgccgt cgccgagggt gccggtcttg
     6841 tcgaacacca cggtatcgac cgcggccagc cgttccagcc cgccggcgcg caccggcagc
     6901 acgccaagcc tggccagggt gctgctggcg gcggcgtagg cggccgggat cgccagcgac
     6961 agcgcgcacg ggcagctgat caccagccac gccagggtca cctcgaacgc gcgctccggc
     7021 gcatgcacgc gccatgccgc gtacagcacg agcgcgccgg cgagttgcgc aaccacgaac
     7081 gcgcgggcga tccggtccgc gcgctgcgcg gcgggcgggc gttgcgcctg cgcgcgttcg
     7141 accagcgccg ccagctgcgc cagccgggtg tcggcgccgg tccggtggat gcggatgcgc
     7201 gcggtgcggc cgtcgcacag cgagccggca tacaccgtgt ccccggcgcg cttgggttgc
     7261 ggctgcgatt cgccggtcag cagcgcttcg ccgaaacgcg ccccggcatc gagcagcacg
     7321 ccgtcggccg gtgccgcctc gccggcggcc acgcgcacga tgtcgccgac cgccagttgc
     7381 gccaggggta cggtttcgca gccgccatcg gcgttttcgc gcagtgccag caccggccgc
     7441 gcgcgcgcca gtgcatcgac ctgggcgttg gcggtggtgc gcacgcgctg ttccagcatg
     7501 cgcgcgacca gcagcaggaa cacgaacatc accgcggcgt cgtaccagac gtgcgggcca
     7561 ccgcgcacgg tttcggccac gctggcgagg taggcgagca gggtcgatgc ggcgaccagc
     7621 gtgtccatcc ctggcgcgcg ttcgcgcaac tcccgccagg cgccggcgag gaacggccag
     7681 ccggcgtaga acaccaccgg ggtcgcgacc aggaaggtga tccagcgcag gaagtcgcgc
     7741 atcggcagcg acatctggtt ggcggtgtcc aggtacagcg cctcggcgaa catcatcgcc
     7801 tgcatgctgc ccagcccggc gatgccgacc cgcagcagcc agcggttgcg ctcgcgccgg
     7861 cggccgcgct cctgcgcgtc gccggcggcc aggtagggct ggtagcccag cgcctgcagc
     7921 cgccgcagcg gccgtgacag cggggtacgc gccgggtccc aggcgatccg gatgcggccg
     7981 gtgaccgcgt tggcggcgat ctcgaggacg ccttcctcgc gtgccagcgc gcggtcgatc
     8041 agccacgcgc aggcggcgca gcgcatgccg tcggtgacca ccaccagctc gcggccaccg
     8101 tcgatggcgc gcgcatgccc ggccagcacg tcctcgcggt cccagtgcgc cagcgcggct
     8161 gcatccacgt cgatccgccg gcccggctgt tcgcgcaggc ggtagtagtc gtcgaggtcg
     8221 gcgtcgcgga tccattgcgc ggcggctgcg cagccgttgc agcagaaccg gcgcaactgc
     8281 ccgtcgagct gcagggaagc tggcgtggcg ggcagcgcct cgccgcaatg gaagcatcca
     8341 ctggccgcgg gaagcgtcgc tccgacgccc gccgcgacgg cattcacggc gccaccagcg
     8401 cgggctgcag gcggacctcg cgctggccgc gcggaagccg tccctgcagg cgccagctgc
     8461 ggtcgatcgc gccgacggtc agcgtccaat cgtgttccag cggcagcgcc tggcgcgcct
     8521 gccaaccacc ggtggccggt tgcagcagca tggacaggtc gtcccgcgcg cgcgccggat
     8581 gctgcaggtc caggcgcagc gcggcatcgc gcgggaacac gcctgccagc ggcagcaact
     8641 cgatccgttc gccgtcgatc cgcagcaccg cgaccagccc gcgttcacgc gcggcgatgt
     8701 ccggggcaag gtcggcgacc tggatctgcg cggtgcgccg caccgggtcc gcgaccacgt
     8761 cgttgttgcc gctggagcgc gccgcggtga ccagcagcgc caccgaggcc agcaccgacg
     8821 ccgccgggat cgcgaccacc agccacacca tcggctcctt ccagggcggg cggccaccgg
     8881 cgccgcgggg cgcgtccatc acaacggccc gaagaagctg ctctcgacgg tttcgctggc
     8941 gtcgccaccg atcgcgctga cctcgaagcg cagcgggtgg cggccgcgga tgccggccgg
     9001 cgcgaccatg gtcagcggca ccgaggccac cgaaccggcg gcgacgtcga ccgtcggcgc
     9061 ctcgcgcagg gccaggccct tgctgtcgct ggtgaactcg atccggtagc gctgcgcctg
     9121 gtcggtcttg ttgacgatct tcagggtgta gccgttctcg atgccggccg aagtttcgcg
     9181 gtacagcgcg ttgcggtcgc gcaggacctc cgcgatcagc ggcgcgcggt tggccacgcc
     9241 ccagccccag gccagcacca gcccgagcag gatggtcccg tacagcagga tgcgcgggcg
     9301 cagcacgcgg gtcggcttgc cctcgatcgc gttctgggtg gcgtagcgga tcaacccctt
     9361 cgggtagccc atcttgtcca tcaccgcgtc gcaggcatcg atgcaggcgc cgcaggcgat
     9421 gcactcgtac tgcaggccgt tgcggatgtc gatgccggtc gggcagacct gcacgcacag
     9481 ggtgcagtcg atgcagtcgc cgagttcctc cggcgcgaat ttcggcagcg gcgcggcctc
     9541 ggtgccgccg tcgaaggtga tcgtgccgtg cgcgtgcgcc tgttgcgcgg cggcgctggg
     9601 gtggcggctg gcgcggaaca cgtagtcgta cgcagcggcg gggtcgagca ggccgcgcgc
     9661 gcgctgcagc acgccgggca ggccgcgctt gcgctggccg cgcggctcgc cgcgcatcgg
     9721 gtcgtaggcg atgatcaggg tgttgcggtc gaacatcgcg ctctggaagc gcgcgtacgg
     9781 gcacatgtac ttgcacacct gctcgcgcag gaagccggcg ttgccccagg tggccagcgc
     9841 gtagaagaac acccagaagg cctcccagcc gccccactgg aacggccacg cgcgcgcggc
     9901 gaggtcgcgg atcggggtga agaagccgac gaaggtgaac ccggtccaca gcgcgaacag
     9961 cagccacacc gcgtgcttgc cgcccttgcg cagcagcttc tgccgcgtcc acgggccggc
    10021 gtcgagcttc atgcgcttgt ggcggtcgcc ctcgatcgcg ctttccatcc acaggaacac
    10081 ttcggtccac accgtctgcg gacaggcgta gccgcaccac aggcgcccgg ccagcgcggt
    10141 gaagaagaac agcgccagcg cggcgatcat cagcagcatc gccaggaaca ggaagtcctg
    10201 cggccagaag gtcagtgcga agacgtggaa cttgcgcgcc ggcaggtcga acagcaccgc
    10261 ctggcgcccg tcccacgaca gccacgggaa caggtagtac atcccgagca gccagaacac
    10321 cgcggccacc cgcagccggt tgagcgggcc ggagacgtcg cgcgggtaga ccttgcgctc
    10381 gctgacgtag gcggcgccgc cggcgtccag gacttccagg gggatctgct tgttgcccat
    10441 gggcttcagt gcggtggcag cgggcggttg aagcggctgg ccgggcgcag caggatccat
    10501 gtgaacaggc tggatgccgc ggtggccatc cagaacatga agaagccgat gctgtagccg
    10561 aggccgcggc tgatctcgat gtgcgggaag gtcatgtcgc gcagcgccag cgggtcgacg
    10621 aaggcgaaga acaccatcgt cgccaccccc gcggcgaaga agctcggcca caggatcgcg
    10681 ccgatcctct gcgccatcgg gcgtggcggg tggtcgaact gcggcgcggt atggtcagcc
    10741 attggcggtc gcggtcatca gggggtcgcc gcggcctgcg ccttgccgtt ggacagcgac
    10801 cacgcgtagg ccgcgaccag gcgcgcgcgg gtctcgccca gcagctcgcc gtgcgccggc
    10861 atcaccccgt ggcggccgtt gatgatggtc tcgcgcagcg ccgcggtgct gtcgccgtac
    10921 agccagtagc cgtcggtcag gttgggcgcg ccgagcgcct ggttgccctt ggcgtccacg
    10981 ccgtggcagg ccacgcagac cgcatcgaaa tgcttcttgc cctgcgccgc catgtagttg
    11041 ttctgcaggc ccttttccgg gctggagagc gtgcgcacgt aggcgaccac gttgtccacc
    11101 gcgttgtcgc cgcccatccc ggccagcacc tggccccacg gcgccatcac gccctcgcgg
    11161 ccttccagca cggtctgcag cacctgttcc gggctgccgc cccagtgcca gatgtcgtcg
    11221 gtgaggttcg ggtagccgat cgcaccctgg ccggcggagc cgtggcaggt cgcgcaggtg
    11281 ttgccgaaga tcgagcggcc cagcttcaat gccgccggat cgcgcgccag cacgtcgatc
    11341 ggctgcccgc ggtagggggc gaaggtggcc tcgagcttcg cgttgtaggt cgccttgtcg
    11401 cgcgcgtgct cgcccttgga ggtccacttg ccgtagccgg cgaacgcgcc cagtcctggg
    11461 taccaggcca ggtagccgat gccgaacagg atggtcaggt agaacccgtt gatccaccag
    11521 cgcggcagcg gcttgttgta ctcggtgatg tcgccgtccc agacgtggct ggtgtcctcc
    11581 ggcttcgggt caccggggcg gcggcgcgcg gtccaccaca gcagccaggc gcaaccggcg
    11641 atgttgagcg cgaccagcgc gatcacgtac cacgaccagc cctgggtcat cgcgcgtcct
    11701 ccaccgggtt ttcggacagc gccaggcgcg cggcctcgtc gaaggccggc ttgcggcgcg
    11761 ggctccaggc ccaggcccag ccggcgatga acaccaccag caggatcgcg gtgacgattc
    11821 cggacaccat ctcaacctcc cttcggggcg tgcttgccca ggccttgcag ataggccacc
    11881 acggcatcca gctcggtctt gccctgcacg tcggccgcgg catccgcgac ctcggcatcg
    11941 gtgtacgggt cgccgaggcg ctgcagcgtg cgcatgtgct gctgcacggt cgccccgtcc
    12001 agcgcgtttt cggccagcca cgggaagctg ggcatgttgg attccggtac caccgcgcgc
    12061 gggtcgagca ggtgcacgcg gtgccagtcg tcggagtagc ggccgccgac ccgggccagg
    12121 tccggcccgg tgcgcttgct gccccactgg aacgggcggt cgtacacgga ctcgccggcg
    12181 agcgaatagt ggccgtagcg ctcggtctcg aagcgcaggg tgcgcaccat ctgcgaatgg
    12241 cagttgtagc agccttcgcg cacgtacacg tcgcgccccg ccagttccag cgccgggtac
    12301 ggcttgaccc cgggcagcgg ctggatcgcc tcggcctggt acatcagcgg gacgatctcg
    12361 gcgaggccgc cgaacgagac cgcgaccgcg atcagcaccg ccagcaggcc gacgttcttt
    12421 tcgatcttct cgtgcggatt gtgttgttcg ctcatgggat cctcaggcgc gcgcttcgga
    12481 ggcgtcgggc ggcagcagcg gctgcgggac gatggcgccg gcctgctggt aggtgcgcag
    12541 cacgttccag gccatcacca gcatgcccgc cagcaccagc aacccgccga acaggcggcc
    12601 gaggtagtac gggtaggtgg cgttgagcgc ctcgacgaag gtgtaggtga gggtgccgtc
    12661 gtcgttggtg gcgcgccaca tcaggccctg catgaccccg gcgatccaca tcgaggcgat
    12721 gtagaacacc acgcccagcg tgtgcaccca gaaatgggcg tcgaccagct tgaccgaatg
    12781 catctgctcg cggcccagca ggcgcggcag catcgagtag atcgagccga tcgagatcat
    12841 cgccacccag ccgagcgcgc ccgcatgcac gtggccgacc gtccagtcgg tgtagtgcga
    12901 cagtgagttg accgtcttga tcgacatcat cggcccctcg aaggtggcga tcatgtagaa
    12961 gctcagggcc acgatcagga acttcaggat cgggtcggtg cgcagcttgt gccacacgcc
    13021 ggacagggtc agcacgccgt tgatcgcgcc gccccacgac ggcgccagca ggatcagcga
    13081 gaacaccatg cccaccgact gcgcccagtc cggcagcgcg gtgtactgca ggtggtgcgg
    13141 accggcccac atgtacaccg cgatcagcgc ccagaagtgc acgatcgaaa gccggtacga
    13201 gtagatcggg cggccggcct gcttgggcac gaagtagtac atcatcccga ggaagccggc
    13261 ggtcaggaag aaaccgaccg cgttgtggcc gtaccaccac tgcgccatcg catccaccgc
    13321 gccggtgtac accgagtacg acttggtcag gctggccggc agcaccgcgt tgttgacgat
    13381 gtgcagcagc gccaccgcga tgatgaagct gccgtagaac cagttggcca cgtagatgtg
    13441 ctggacccgg cgcctggcga tggtgccgaa gaacagccag ccgtaggcga cccagacgat
    13501 ggtgatcagg atgtcgatcg gccattccag ttcggcgtat tccttgccct ggctgaggcc
    13561 cagcggcagg gtgatcgcgg cggccacgat caccagctgc caaccccaga acacgaagct
    13621 ggcgaggccg tcgctgagca ggcgcacgtg gcaggtgcgc tggaccacgt gcaggctggt
    13681 ggcgaacagg gcgctgccgc cgaacgcgaa gatcaccgca ttggtgtgca gcgggcgcag
    13741 gcggccgtag cccagccacg aaatcccctc gaacagggtc ggccagtaca gctgggcggc
    13801 gatgatcacg cccaccagca tgccgacgat gccccagacc acggtggcga cggcgaactg
    13861 gcgcacgacc ttgtcgttgt aggttgcggt gatggcctgc atggacgact ccgttttcca
    13921 gtgcgtcatt ttaggcgagc cggggctggc gcgcacttga tctggcgcaa atcgcggcgg
    13981 cggacgcgtt ggcacgggca tgcgccgcga agatcgcgga taatccgcgg catggccgcc
    14041 aatgaccgtg ctgcgatccc gttggacctg gtgaggctgc ggcgcagctg tgccgagtgt
    14101 tcgctgcagc agctgtgcct tccagccggg atcgaagggg ccgacctcgg ccgcttggaa
    14161 gcgctggtgc agcgcccgcg atcgttgcag cccggggatg cactgttccg gctgcacgat
    14221 ccgctcggtt cggtgtacgt gaccagcgag ggcgcgttca agaccatcgg catcaacgaa
    14281 gccggcgagg aacacgtgct gggcttccac ctgccgggtg aattgttcgg cctggatgcg
    14341 atcggcagcg gtcgccatcg ctgcagcgcg atcgcgctga cccaggcgcg ggtatgcgag
    14401 ttgccgttcg cgaccctggc caccgcggcc acccagctgc ccagcctgca acaccagttg
    14461 ctgcgggtga tggggcagag cgccgacctc gaccacgacc acgtcgacct gctgtcgcgg
    14521 cgccaggcca gcgagcggat cgcgctgttc ctgcagggca tgggcgagcg ctaccggcgg
    14581 atcggccgct ccggcgagga tttccagttg ccgatgagcc gcgacgagat cgcgcgctac
    14641 ctcgggctgg cgctggagac cgtcagccgc agcttcagcc gcctgcacga ggatggcgtg
    14701 ctcgacgtgc gcgggcggcg ggtgcgggtg ctggacgcca agcggctgca cgcgaccgcc
    14761 agcggctgcg aggccgacat cgcccgcgcc gcggcgcgtc cgcgccgggc ctgagcgcgc
    14821 gtcccgcccg ggcatcacgg ggtggcgtga tcgtgcgggc aattgagcca tgtcaaggca
    14881 ggccgcgcgc gaccgcgcac acttcgctgg ccggagccac cggcatgcgc aggagctggc
    14941 catgcacgac atcctcgtcc atgcggttcg ctaccgtgac cgcgacccca cggcgtccta
    15001 tgcggcgcgg ctggcggcct ccctgcgcgc ctcgctgacg gcggtgttcg tgccgccggc
    15061 gatgcccgcg atccccgaat acgacgtggc ggcggtgatc gccgaatacg cggcgtggat
    15121 cgagcgcgag acccgccagg cgcgcgccgc cggacccggc ttcgaggcgt ggtcggcgtc
    15181 gctgggggtt gcgcacgcgc gctgggaggt ggcggacgga caggtcgcgg cggtgcttcg
    15241 ccatgccggc cgctggcacg acctgctggt gctcggcacc ggcagcgagg acgcctggca
    15301 gtccgaggcg ggggtgtccg aactggtgct ggccaccggc ctgccgtgcc tggtggtgcc
    15361 acctgcggcc aacgattgcg cgctgccgca cgcctgcgtc gcggtggcgt ggaatggcgc
    15421 ggccgaggca atcgccgccc tgcacgcggc gctgcctctg ctgcagtcag cgcggcgggt
    15481 ggtcgtgctg tccggcgagg ccgcgcctgc ggcgccgggg ctggtgccat tcgacatcga
    15541 tgcctgggcc aggtgccacc aggtggaggt cgaatacctg gcgatggacc gcgacgagga
    15601 cgtcggcgcg gccctgcttg ccgccgccgg cgaggtgtcg gcggacttgc tggtcgccgg
    15661 tgcctacggg cgttcgcggc tggccgaatg ggcgctgggc ggggtcaccc gccacctgat
    15721 ccggcacgcc acgatcccgc tgctgttgcg gcactgacgc cgggggtcag cgcgcggtgt
    15781 agccgccgtc gatcaccagt tcgctgccgg tgacgaactt ggattcgtcc gaggccaggt
    15841 agaccacgcc ccaggcgatg tcgtccgggt cgcccatgtg gcccagcggg tgcgccaggc
    15901 cgacctcgcg gcaggccgcc tcgacgtcgg cggcgccgct gtcgcgcagg tggtgctcga
    15961 ccatcggcgt gcggatgaag cccgggtgca ccgagttcac ccggatccgg tcgggcgcgt
    16021 aatacagggc atcggtcttg gtcatcagcc gcaccgcgcc cttggacgcg tggtacggcg
    16081 gcacgtcggc ggcgccgacc aggccgtaga tcgaggacag gttgatgatg ctgccgtggc
    16141 cggcgcgctg catgtgcggg atcgcgtgct tgttgcagaa gaacacgccc ttgacgttga
    16201 ccgcctgcac ccggtcccac tcggcttcgg tcagctcatg ggtgggcttg ttggtgccgg
    16261 cgatgccggc gttgttgacc agcacgtgga ccccgccgaa tcgctccgcc accgcgttga
    16321 tggcgtcgcg caccgcggcc tcgtcggcga cgtccacgtg ccagtacccg gcttcgtggc
    16381 cggcggcctg caattcggca agcagggcgt ggccttcctc gtcgagcatg tcgaacaggg
    16441 cgacgctggc gccttccccg gccagccgca gcgcgcaggc gcggccgatg ccgagcgccg
    16501 cgccggtgac gatgcaggtt tttccgttga cgcgttgcat gaccaggacc tccggtgtgt
    16561 tgttgcgatg gacgtgcgac cggtcccagt tgagcgcagc cgcgcggctg cgtccttgac
    16621 cccggtcaac cagcggtggc gggaaccccg gcgtccgggg cggcgggttc gcggcggccg
    16681 gccttgagca gcgcggcaac ggccagcggc aggcacaccg ccagcagcca cggtgccagc
    16741 ggtggcggtt cgaagccgaa ccagcccgcc gcaagcggcc agcgggtcac cagcagcaac
    16801 accgccagcg ccagcgccga gaccaccgcg aacgcgcggt tgcgggcgcg cagggtctgc
    16861 cacaacgagt caccggcgcg gtagaccacg atcaggccga ggttgccggt gaccagcgcg
    16921 gtgaacgcca gtgcgccaac ctgcgccgcc ggcagcgcct gcgccagtcc cagcgcatac
    16981 accgcggcca ccgcggcgaa catcgccgcg ccgcgcagca ggttggcgaa caggccgcgc
    17041 acgccgagca ggcgcgcctc ctgcgaccgt ggcgggcgcc gcatgatgtc cgcgccggcg
    17101 gcctcggatt cgcgctcgaa cacgatgctg caggccgggt cgatgatcag ttcgaggaac
    17161 accacgtgca gcggcaacag caccagcggg ccgccgccga ccagcggaag cagcgccagc
    17221 ccggcgatcg gcacgtgcac cgcgaggatg tagtgcaccg cgcgccggat gttgtcgtag
    17281 atcgtgcgcc ccagccggat cgcgcgcacc acggtcacga agtcgtcgtc cagcagcacg
    17341 atcgccgcgg cctcgcgggc gacgtcggtg ccgcgctggc ccatcgcgat gcccacgtgc
    17401 gccgacatca gcgcgggggc gtcgttgacg ccatcgccgg tcatcgccac cacctggcca
    17461 tcgcgcttga gcgccgccac cagccgcagc ttctgctccg ggcgcacccg cgcgaacacg
    17521 ttgctgccgg tggcgcatcg cagcagtgcg tcgtcgtcca tggcttcgag cgggtggcct
    17581 tcgaccacgc cgcgcgtgcc cgccgcgatc ccggcctggg ccgcgatcgc gcgtgcggtg
    17641 gcgggatggt cgccggtcag catgaccacc cggatcccgg cggcgtgcgc ctccgcgacg
    17701 gcttccacaa cgccttcgcg caggggatcg gcgaacgcca ccagcccgcg ccagccgaag
    17761 tcgaagtcgc gcatcgatgg cggcagggcg gcgtcgccgt gccagcgcgc gtccgccacc
    17821 gccagcaccc gcaggccgcg ccggcccatc gcgtccacct cggccagcag cgcggcgcgt
    17881 ggcccggctt cgacgtggca caggccggcc acggtctcgg gggcgccctt gcaggcaacc
    17941 aggatctccg cgccgcgccg ccaggcgtga ctgaccgcgg tgcaggcggg cgtcagcggg
    18001 tattcgcgca gcaggtccga ggcgtccggg cctggcgcga tcgggtgggt gccgagcgca
    18061 gccaccgcgt ccgccagtgc gcggtccatc ggatcgatcg aatccgcgcg gctggccagc
    18121 actgccgtct gcagcagcgc ctgcagggca gggtccatgg tcggacgcgg cggtgcgcgt
    18181 cgatcgccgg ccaccagttc ggcgacggcc atgcggttgc gggtcagggt gccggtcttg
    18241 tcggtgcaca gcaccgtgat cgcgcccagt gcctcgatcg ccggggtgcg ccggaccagc
    18301 gcctggtgcc gcgccatccg ccatgcgcca agcgcaagga acaccgccag taccatcggg
    18361 aattcctcgg ggatggtcgc gatcgccagg gtcaggccgg ccagcaccgc ctgcgtccag
    18421 tcgccgcggg ccagcagggt cagcgcgacc accgcgatcc cggtggccag cgccagcgtc
    18481 gcgaacagca ccaccacgcg ccggatctcg tgctgcatcg gggtcggttc gacgtgcagg
    18541 ctgcgcagcg agaccccgat cctgccgacc tcggtgcggg cgccggtcgc caccacttcg
    18601 gcgatgccgt ggccgctgac caccagggtg ctggcgtgca ccagcgccgc ttccgctggc
    18661 ccgccgatgt ggcgccgcac cggcaccgat tcaccgctca gcagcgattc gtcgacgtgc
    18721 aggtcggtgt cttcgagcag gcgcgcatcc gccggcaccc ggtcgccctc cgcgagcagg
    18781 atgacatcgc ccaccaccag cgcgccggcc ggcagcaccc gcgggctgcc gtcgcgcagt
    18841 acccgcgcac gtgggctgcc gagttcgcgc agggcctcga gggcgcgctc ggacttctgt
    18901 tcctggtaaa tcgtcagtcc gatcacggcc aacaccgaga ccgccagcag cagcgcttcg
    18961 ccggcatcgc cgagcaccag gtagaccgcg gtcgcggcca gcaggagcag cagcatcggc
    19021 tcgcgcagca cgctgagcac gatcgcccag cgcgaacgat gctccgcctg cggcagtgcg
    19081 ttggggccgt cgcgcagcag gcgcgcggcg gcatcggcgg aaccgaggcc acgcaacggc
    19141 gcggcttcgg cgcggggcgc ggactccgcg gcggcctgca tgctcgacgg cctcattgcc
    19201 ggatcggtcg ttatcggcgc gcgccagtca cgcgagctcg acctgttcga ggtactccgg
    19261 gaaatgcgct ggccagccga gttcgtgctt gatgcgcgtg cgcaatgcgt cggcggcatc
    19321 gggttcgccg tgggtcacga acaccccgcg cggcggtgtc ggcgcggtgc gcagccaggc
    19381 caggatctca gaggcatcgg catgtgcgga gccggcggcg agttgcacca cttccgcgcc
    19441 gacctgcact tcctgcccga agatgcgcaa gtgcttctgg ccggcggcca gcgccgcgcc
    19501 gcgggtgccg ccggcctgga acccgcacag gacgatggcg ttgcgcggat ccggcgcgaa
    19561 cgcgaccagg tggtgcagga cgcgaccgcc ggtggccatg ccgctggcgg agacgatgat
    19621 catcgggccc ttgtgccggt tcagcgcctt cgactgctcg acgctttcga ccacgtgggt
    19681 ggtgcgctgc atcgcgtcga gttcggcggc atccagccgg tgcaggcgcg ggaactgccg
    19741 gtacaggccg gtcacgctgg ccgccatcgg gctgttgagg tacaccggca ccttcgccag
    19801 ccggccactc gcctgcaact gggtgatcac gtgcagcagc atctgcgcgc ggccgaccgc
    19861 gaacgccggg atcaccacca cgcccttgcg cgcgaccacc cgctgcagca ccgccagcag
    19921 ttcctcttcc agtccgtcgg cggggtgcgc gcggttgccg taggtcgatt cgaccaccac
    19981 ccagtcggac gccgccagcg acgccggggc cggcatcagc gggtcgtccg gccgtccgag
    20041 gtcgccgctg aaggtgatcc ggcggccttc gaattccagg gtgacagccg ccgcgccgag
    20101 gatgtggccc tgcgcgcgga agcgtgcatg cacgccgggc actggctcga acccatggtc
    20161 gaagccgacc ccgcgcaggc gcgcgaggct ggcgcgcgca tccgcctcgc tgtacagcgg
    20221 ctcggccggg tggtgcttgg accagccgct gtggttcgca tggccggctt cctcctccag
    20281 cagccggccc gaatccggca gcaggatgcc gcacaggctg cgggtggcct cggtgcacca
    20341 caccttgccg cggaacccgg agcggaccag ccgcggcagg tagccgctgt ggtcgaggtg
    20401 ggcgtgggtc aacagcaccg cgtcgatcga cgccgggtcc accgggaact cggcccagtt
    20461 gcgcaggcgc agcggcttgt agccctggaa caggccgcag tcgaccagga tccgggcgtt
    20521 gccggcggtg accaggtggc gcgaaccggt gacggtggcg ttggcgccga ggaattgcag
    20581 gctgaagcgg ctgggcttgc tgtgcgcgtc ttgcatcggg cgtgtcctcg cacccggcgg
    20641 gtgctggccg atcgtgggtg aagcctgatc ctaccccgcc cacactgcgc gcagtgtgag
    20701 gcggttgcgt tgccggggct ggaggcacca aggggccgcc cgcggtagcg atgcgccggc
    20761 tgcttgatgc aggtcatcgc gccggcgacc gccgcggacg acgcttgcgc cacccgccgt
    20821 ggcggcaacc gccacgacag cccccatcgc gtgccgaggc acgccacccc ccaaggaaaa
    20881 gcatggaaac catccgcttg agcacgatcc ccgccgctgc cgaaacgctg catccgttcg
    20941 atgcccgtgg catcggcaag cgcttccgcc acgcggcgat cttcggcgcg ctggatgcgc
    21001 tgagcccggg cgaggcgatg cgcttcgtca acgaccacga tccgctgccg ctgctggcgc
    21061 aattgcagca gcgctatggc gatgcgcttg cgatcgccta ccgccagcgc gagcccggcg
    21121 cggtggtcat cgacttcacc ctgcgcgccg gccacggcgt tggctccacc tgctgcggca
    21181 gttgcggctg atccgggcct gctcgcgccg cgtaacccat ccgcggcgcc cgccgggctt
    21241 gggccggggc ttgaggctag tcgccgggtg tggtcgcggc cgcggcgttc atgccagcca
    21301 cgccagcggg tggtgcgcgc gggcgatgcc gtacatcagg gcgaaggtcg ccagcgccgc
    21361 ggcgaaatac agcgcgcgga cgcggcgcgg gcgctgcggt cgcagtgcca gcaccccgag
    21421 caccacgtag gcgaacagca gggcgatctt cgcgagcagc cagccgttgg cgaacaccga
    21481 ccacggcagg atcgccacca gcagcagcgc cgcggtgagc agggaggtgt cgatcgcgta
    21541 ggtcagccac ttcagcggcc gcgcctgcgg ccagcgtgcg ccggccagcg cggccacgcc
    21601 acgcagcgcg aagatcgacc cgctcagcag caccgcgaag atgtggaaca gccggatctg
    21661 cggatagaac tcgatcatcg cgccaccccg gtcagcccgg cttgccgtcc gtgcgtggac
    21721 tcaggtagat ccgtcccagc ctcgccaccc acggcgcgaa cgccagcagc cagccgatcg
    21781 cggcgcaagc catccagccc atcggatcgt ccgcaagttc ggcggccacg cgcaccattg
    21841 ccaccacctg cacgcccgcg aacgcccacc aggccgcggc aggcatcacc agcggctggc
    21901 cggagtggcc ctgggtcacc cgcgtcacca tcgccaccag cacgctgccg aagaacccga
    21961 tgaacagtgc atgcgccggc gcgcggccga gccagtggat cccggtcatc gcgtaggccg
    22021 cactctgccc cgcatacagg gcaaaggtca gcggcagcca cgacaggccg atgaacagca
    22081 ccgccagcag gcccggcatg cgcccgcgcg gccacaactt ccaggccatg gcaagggcga
    22141 gtcccagcag gggcaggtcg accacccaca gccacgcgta ggcatgcagc agctccagcg
    22201 ccacgtgcgc cagcagcagc ggccagaagg ccgccagcag ccacagcggg cgccacggct
    22261 ggtagccggc gaccacgttg gtggcgaaga acgggaacat gcggtgggcc acggtcaggt
    22321 acaccggcag cagcacgccg aaggtgccga gcttgatgct ggcgaaggca tacagcgcgt
    22381 tttcgttgag caggaacgcg aaccacagca gcaggcccgc cagccccagc agcagcgcga
    22441 gcatgcagga gcgcgcatgc caggtggtgc cgcgctcgcg ccacagcagc ggcatcaggt
    22501 gggcgatcac cgccagccag ccggccagcg tcatcagcgc accgaccacc atgcccgcct
    22561 cccagccgaa cgcgcccagc agggtcgcca gttgcccgcc gaacatgcca aggccgaccg
    22621 gcaggtagcg ccagcgctcg aggtcgggca agcccatcca gcgcgggaac acggtgagca
    22681 ggaagccgaa catgaagctc ggcaacatct ggtactgcat cacgaatgcg tgcagccagc
    22741 cggcgtaggg cgtcggctgc ggcatcggcc atgcctgcca gcgctgggcg atgacccagg
    22801 ccgtccacca cagcatcgcc agcaacaggt tgctggcgcc gatgaagaac atgagtcggt
    22861 gcggcgcctg cgccagcagg cgcggcgagg gggcaggacg tgcggtaccg gtaggcatgc
    22921 gcgccagtct gccggcgacg cgctggatcc gctttgatcc aggtcagtcc gtggaccgac
    22981 gcagtgcgtg gttcaggcgc gcaggaccgg ggccgccagc gcttccagcg cagcggtgtc
    23041 cagcaattcc agctcacgcc ggtccaccac cagccgcccc tcttcctgga accggcgcag
    23101 cacgcggctg accgtttccg gcgcgagccg caggtagttg gcgatgtcgg tgcgggccat
    23161 ggtcagctgg aagcgggtgg cggagaaccc gcgcgcggcg agccggcgcg acagcccgac
    23221 caggaacgcg gccatgcgct ggtcggcgga ccagtcgccg gccagcagcg cggcacgccc
    23281 gatgtcgcgg ctcagcaggc ggaacagttg ccgctgcagc cccggcaggc gggtggccag
    23341 caccgcgatc ttcgggaacg agaagcggca cagcatcacc gtgtccagcg ccaccgcgtt
    23401 gcacgggtag gtctcgccgt cgatcgcgtt gaggccgatc acctcgcccg gcaggtggaa
    23461 gccgagcacg tgctcgtggc cgtcgcgatc caccacgtag gtcttgaccg tgccggcgcg
    23521 caccgcggcg atcgcctcga acggctcgcc ctcgcggaac aggtggtcgc cggcatggaa
    23581 cgggccgatg tgctcgacca gcacgtgcag gtccatcagc gcgcccttgt ccatgccctc
    23641 gtccatgcac gcctgcgaga acgcgcaggt ggagcagaag cgcaggctgt cgccgtcgtc
    23701 ggccagggtc tgcggactct gccgcgggaa cggcacgttg ctcaagacgc ccaccggcac
    23761 gccctcagat cgcgcgcgag aatcgcggcc tggcgtcggt gacgggttgg tcgaggtagc
    23821 ggtcgaagca catcgcaata ttacgcaaca gcatgcgccc gcgcgaggtc accgtgatcc
    23881 ggtccgcctc gatccgcacc agtccgtcct ccgccagcgg ctgcaacttg gccagcgcat
    23941 cggcgaaata ggcggcgaaa tcgatgccat aacggcgctc cagcgccgcc atcgggattt
    24001 cgccctggca catcagctgc tggatcaggt ccgcgcgcag ctggtcgtcc tcgtccaggc
    24061 gcatgccccg gaacaccggc agccggccct cgtccagcgc cgcctgccag ctcggcaggt
    24121 cgcgcgggtt ctggctgaag ctgtcgccga cgtggctgat cgcggatacg ccaagcccga
    24181 ccaggtcgct gtcggcgtgg gtggtgtagc ccatgaaatt tcggtgcagc ccgccgcgcg
    24241 cctgcgccag cgccagttcg tcgtcgggca gggcgaaatg gtccatgccg atgtagcaat
    24301 agccggccgc ggtcaggcgt tcgatcgcca gctgcagcag cgccagccgg gtttcggcgt
    24361 ccggcaggtc ggcgccgacg atctgcttct gcggcttgaa cagctccggc aggtgcgcgt
    24421 aaccgtacac cgcgacccgg tccgggcgca tcccggtgac gatgtcgagg gtgcgcgcga
    24481 agccctcgcg ggtctgcttc ggcagtccgt agatcaggtc gatgttgacc gagcggaagc
    24541 cgttgccgcg gcaggcgtcg accaccgcgc gggtttcctc caccgactgg atgcggttga
    24601 ccgcgacctg cacctcggga tcgaaatcct gcacgccgaa gctggcgcgg ttgaagccgg
    24661 cggccgccag ttcggcgatg tcggccgggc tgacgaagcg cggatcgagc tcgatcgaca
    24721 ggtcgcgttc gtccgcatcc gagaaatgga agtggctgcg cagggtgtcg accacctcgc
    24781 gcagctgcgc cggcgacagg aaattcgggg tgccgccgcc gaagtgcagc tggatcacct
    24841 cgcggtcgcg gtcgaacagc ggcgcggtca gcgcgatctc gcggtacagg cgcgcgaggt
    24901 aagcctcgcc gcgcgcggtg tcgcgggtga tgatgcggtt gcagccgcag tagaagcagg
    24961 ggctggcgca gaacggcacg tgcacgtaca gcgacagccg gcgcgggatc gggtcgccgt
    25021 tgctggcgct ggcgaccgcg cgcaactccc gcgcaccgaa gcccgcgtgg aactgcggcg
    25081 cggtcggata cgaggtatag cgcggtcccg gccggtcgta tcggcgcagc aactcggcgt
    25141 tgaaggcaat ggcttgcgat tccatgcgcg cagcctaggc ggcgccctgt gcgcgcatct
    25201 tgatctggat caacctgcaa tcccggaaac gaaaaacccc cgcgtcagcg agggttcttg
    25261 gttgtacctg gtgcccggga gaggactcga acctccacga agttgccccc gctagcacct
    25321 gaagctagtg cgtctaccaa ttccgccacc cgggcagggt gcagggggcg tatgttgcag
    25381 tgcgattcgg acgatgtcaa cggatccgcg cgatgccgac ggccgctgca cgggcgcgat
    25441 gtaccatcga cgcatgagca aaccccccag caagcgcggc cctggccgcg gtggcaagac
    25501 acccggcaag accggcccca cccgcagcga aggcacgcca acggggcggc cgggtccgaa
    25561 gaaggccgcc agcaaggcgc cgggatggct gcccgacgcc ggcttgatgc gcgcgttcgg
    25621 cggtgccggc gctggcaagc caggcgcccg gtccgggcac ccgcatgcgg acaccgcggc
    25681 aagcaggcgc gcgcatccgc cgaccgccag gcagccggcg atgccggcca gccctgcgcg
    25741 cgcgggcgac ccgcacgccg cgcgcgaggc gcaacgctat gaacagccga tcgccagccg
    25801 cgagatgatc ctgcaggtgc tggccgccaa cgacggcccg atggacgccg aagcgctggc
    25861 ggccaggctc gcgctgaccg cgcccgaccg cttcgaggcg ctgggcaagc gccttggcgc
    25921 gatggtccgc gacggccagt tgctgcagaa ccgccgcggc ggtttcgccc ccgccgcgca
    25981 gatggacctc gtgcccggcg tggtgatcgc caatccgaac gggttcggct tcctgcgccc
    26041 ggacgcgggc ggcggcgacg acctgttcct gccgccgttc gagatgcgca aggcgatgca
    26101 cggcgaccgc gtgctggcca gcgtcaccgg cgtggaccgc agcgggcgca aggaaggggc
    26161 gatcgtcgag gtgctggagc gccggctcaa ccgcctgctc ggccgcttta ccgaagaaga
    26221 cggcatcgcg ttcgtggtgc ccgacgacaa gcgcatccag cgcaacgtga tgatcccgca
    26281 cgatggccgc aacgaggcca gggccgggca gctggtggtg gtggagatca cccgggcgat
    26341 ggatgccacc cggccaccga tcggcaaggt gctggcggtg ctgggcgaca agctgaccgc
    26401 gtcgctggcg gtggaggccg cgatccacgg ccacgagatc ccgcacgaat tcccgccggc
    26461 ggtgctggcc gaggccaccg cggtgccgct gcaggtggat gccgcgaccg ccgcgcgccg
    26521 ggtcgacctg cgcgcgctgc cgctggtcac catcgacggc gaggacgcca aggatttcga
    26581 cgacgcggtc tggtgcgagc ccaaccgcga cggcttccgc ctgatcgtcg cgatcgccga
    26641 cgtctcccat tacgtgcgtc ccggcacgcc gctggacgat gaagcgcagt tgcgcgcgac
    26701 ctcggtgtat ttcccgggct tcgtggtgcc gatgttgccg gagaccctga gcaatggcat
    26761 ctgctcgctg gtgccgaaga ccgatcgcct gtgcttcgtg tgcgacatgc aggtcggccg
    26821 cgacggcatc gtgaccgcaa gcgagttcta cgaggcggcg atgcactcgc acgcgcgcct
    26881 gacctacacc gatgtgtgga acgcggtggg cgaaggcatc cccgacgatg accgcgatgc
    26941 cgcgctggcc aagattggcg ggctgctgcc gcagatccag cgcctgcacc agttgtacaa
    27001 ggtgctggac aaggcgcgcg ccaggcgcgg tgcgatcgag ttcgagagca gcgaagtgcg
    27061 cttcgtgctg gatgccaagg gcgcggtcag ccaggccggc atgctccagc gcaacgacgc
    27121 gcacaagctg atcgaggaat gcatgatcgc ggccaacgtg caggccgcgc tgtacctgat
    27181 gggcgttggc atcccggcgc cgtaccgcaa ccacgacaag ccgccggaat ccaagtacgc
    27241 ggacctcggc gaattcctca aggagttcgc gctgcgcctg ccgccatggg ccaagctgcg
    27301 cccgaaggat ttccgcgcgc tgctggagaa gatccgcgag cgtcccgatg ccgcgctgct
    27361 ggaatcggtg atcctgcgca gccagtcgct ggcggtgtac gcgccggaca acatcggcca
    27421 cttcgggttg gcgctggagg catacgcgca cttcacctcg ccgatccggc gctacccgga
    27481 cctgctggtt caccgcgcga tcaagcacgc gctggccggc ggcaggcgcg atgcctacga
    27541 atactccccg caccggatgg ccagcctgtc gctgcagtgc tccgagcgtt cgcgccgcgc
    27601 cgacgaggcc cagcgcgagg tcgacgagcg ctatcgcgcg gcgtggatgg aagaccacgt
    27661 gggcaaggaa ttcgagggca ccatcagcgg cgtcaccagc ttcggcctgt tcgtcgaact
    27721 ggacgattcc aaggtcaacg gactggtcca cgtgacccag ctggccaacg acttctacca
    27781 cttcgatgcg gtgcgcaaga ccctgaccgg cgagcgccgc ggccgcgaat accgcctcgg
    27841 cgaccgcgtg cgggtggtgg tgctcaaggc cagcgtggaa gatcgcaaga tcgatttccg
    27901 gctggtcgag gaaggcgtcg cagccgccaa gccgctgccc gagcgtggca agccggcgaa
    27961 gcggcagaag cagaagtact gagtgttgcg caggccgggg cgaagcggcc tgctcctccg
    28021 cttctgcgaa catcctgttc gaggcgcgcc cgccggccat ccatggccgg ctttccgcag
    28081 gccgctccgc cccgacccgc gcaacatcgc ggtgtgtatg ggcacaataa gcgtatgagc
    28141 aagcaagccc aatggatcgt cggcctcaac gcggtggctt cggcgctgga acacgacgcc
    28201 gagcacgtgc gcgaggtgct gcttgaggcc ggcggcaaga acccgcgcat cgtcgagatc
    28261 gaacagcagg cgcggcgcaa ggagatcgac gtccgccgcg tggcccaggg cgcgctggat
    28321 ggcgtctccg gcggcgtgcg ccaccagggc gcggcggcgc gctatgcagc ggcgaagacc
    28381 tgggacgagc acgaactggt cggcctggtc gaagcggccg gcggcaaggc gctggtgctg
    28441 gtgctggatg gcgtgcagga tccgcacaac ctcggcgcct gcctgcgcag cgcggcggcg
    28501 gcgggcgcga ccgcggtggt gttcccgaag gacaaggcgg tgcaggtcaa cgccaccgtg
    28561 cgcaagacct cggccggcgc ggcggacacg ttgccggtct tcagcgtcac caacctctcg
    28621 cgcaccctgc gcgacctgca gaaggcgggg gtgtggatct acggattcgc cggcgaagcc
    28681 acggcttcgc tgtacgcgct ggacctgaag ggcaacgtcg cgctggtgat gggcagcgag
    28741 ggcgacggcc tgcgccggtt gacccgcgag aactgcgacc agctggtgaa gatcccgatg
    28801 cccggcgagt tcgagagcct gaatgtctcc gtcgccaccg gcatcgcgct gttcgaagcg
    28861 gtccggcagc ggcagccgta gcacctgggg gaaatgacga tggcgctgga cttggcgtgg
    28921 tacgactggg tgggcctgct gggcaccgcg atgatccttg gcggctttgc gctgctgcag
    28981 gcgggcaggc tgtccggcac cggactggtc taccagttgc tcaacctgtt cggcgcggcc
    29041 ggcgtgctgg tgtccttgct gggcaccttc aacctgtcgg tgttcctgct ggaagccgcg
    29101 tggatcgcgg tcagcgttta cgggattgcg cggtcgttca aggagaagcg caccgaagcg
    29161 cggtgagtcg ccgggcgccg tgcccgaagc ggcttgggac acgccgtgaa tacatccatg
    29221 taggctaatc ggcgccatcc atggcgccga ttgtccctgc gctcgcttcg ggcatggcgc
    29281 ccggcgacgg gccacatcga ggccacgcat tcgcgatgat gcagaacagc tgcgcggtgc
    29341 gttccgcgtc gtagaccgcc gaatgcgcct cgcgctggtc ccagtccagg ccggcggcgt
    29401 ggatcgcgcg ggccagcacg gtctggccgt acatcagccc cgccatcgtc accgtgtcga
    29461 acacgctgaa cgggtggaac gggttgcgct tgtgctgcac ccggttgacc gcggcgttca
    29521 ggaaattgag gtcgaagtgc gcgttgtggc cgaccaggat cgcgcgctgg cagccgtgct
    29581 tcttcgccgc cgcgcgtacc ggcacgaaca ccttgtccag gccctcgcgt tcatccacgg
    29641 cgaagcgcag cgggtggccg atcttgatgc cggtcacttc cagcgacttg gggtcgatcg
    29701 aggtgcccgg cgccggttcg atgtgcgcat ggcaaacctc gcccggcacg aagcggccgt
    29761 gcgggtccag ttcgatcggc accgccgcga tttccagcaa tgcgtgcttg ttccagtcga
    29821 agcccccggt ttcaacgtcc accaccaccg gcaggtagcc gcggaagcgc tcggccatcg
    29881 gcttgaaggc cggcgtttct tggatctcgg tcatcgcgac aggttaatcg atgcgggtgc
    29941 cgagcaggac gccttcgttg cgcaaccgct gcagcatcgg cgtggcctcg gccaggaagc
    30001 cgtcgaaatc ggcgtggcct gcttccatcg cgagttgccg catcagccgg cgtccgtcca
    30061 cgccggcatc ggtatcgacc agctccagca ggcggaacag cagcggcgac agcgcggaga
    30121 aatggatgcg gccatcgggc ccgcggcgca acaggagcag tgtcggctcc ggcggcggtt
    30181 cgcctggttg gtactccggc gcaaggcgat tgaccggcca gcgatacgcc agcggccacg
    30241 caaggggcga gagcatgggc acgcctgcca gcaggtctcc gccgggatcg tgagggggca
    30301 ctaccgcatc gctcagctgc aggccaagtt ccgcccattc gtaatgcgcg agttccgcga
    30361 gccacgggcg ccgcggatcg gtttcggctt cgaggaaggc gatcagctcg cggccgagtt
    30421 cggtgaacag tggcgtatgg cagcggtggc gggcaaggaa gccagccacc agcgcctgcc
    30481 agccggcatc gccgaggatc ttgcggatca ccgggaagtt gccggccagc aaggcgtcga
    30541 ggttgttgta gacgaggccg cggtagatcg ccaggcgccg ggccgcgatg cccggcggac
    30601 ccgggtgcgc ggcgggatcg cgaacgtgcc gcgccatcgc atcgagttgt tcgcgcaggg
    30661 tgctcaccgg aaccgcgcct gcaggtcgcg gatgcgctgc acttccgcca gcaggaccgg
    30721 caggggcggg aggttgaagt cgcgctcgag cagggtcggg cgcacgccgt gccgctggta
    30781 ggcatggccg agcaggtccc acaccgcgtc cttcaccgcc gcgccgtggg tgtcgatctt
    30841 gaggtcgtcg gcttcgtcgt aatggccggc gacatggatg cttgcgatcc gcgccgacgg
    30901 catcgcgtcg acgaacgcgc gcgggtcgta gccgtggttg atggcattga cgtagacgtt
    30961 gttgacgtcg agcaacaggt cgcagtcggc ctcgcccagg accgcttcga tgaacgcgat
    31021 ctcgcccatc gcctggaagg gcgccgcgta gtagctgacg ttctcgatcg cgatgcgctg
    31081 gcccagcgcg tcctgcactt gcgcgatgcg tccggcgacg tggcgcaccg cggcctcggt
    31141 gaacgggatc ggcagcaggt cgtagaggtg gccggaggcg gagcagtagc tcaggtgctc
    31201 gctgtaggcg acgacctggt ggcgggcgag gaagtcgcgg gtctggcgca ggaacgtggt
    31261 gtccagcggg tccggcccgc ccagcgacag cgacagcccg tggccgctga gcggatgccg
    31321 ggaggccagc gtcgccagct tgtcgcccag cacgccgccg acaccgatcc agttgtcggg
    31381 cgcacactcc agaaaatcga aggcgcccgc aggcgcctcg atcaactggt ccagcagggc
    31441 cctgcgcagg ccgaggccgg cgctatgcgc tggcaatggc tggcgcatgc cggctcaggg
    31501 gttgccgccg cacttgcctt cgccgcactt gccctcgccg cacttgcctt cgccgccctt
    31561 gctggcggcg tcgtgcttcg cgtccttcat ctgggcgtgg tgcgcatcca tctcggcctg
    31621 ggtgatgaag ccatcgccat tgccgtcatg gccggcgaac ttctcttcgc tgcccttgtg
    31681 cgcggcatcg aattcggcgc gcgacaggcg gccatccttg tcggtgtcca tcgaggccaa
    31741 ggtatgcgcc ggcgcgccgg ccgcgggttg catgcccccg cagttgccct cgccggcctt
    31801 ggcttcggcc ttggcgggat cctgctggta gccgccggcc atcgcctgca tcgtgaacag
    31861 cgagccggcg accagggtac cggtggcaac ggcggcgccg atcaccaggg cgatcggctt
    31921 gtgggactgt gacatcgtgg ctccttggcg cggttcgcgc gtgacgtgtg gcgcgatcct
    31981 accgccagca cacggccacg gccagccgct ggcggccggc gttgccgccg gtacagcggg
    32041 tcgcggcggt cagaccaccg tggtgctgct ctcgtcgcgc ttgtcgcgcg gcggcatcag
    32101 ttcctcgcca tgcacgagga accacacgtt ctcggcgatg ttggtggcgt ggtcgccgat
    32161 ccgctccagg ttcttggcca tgaacagcag gtgcgtgcac ggggtgatcg cgcgcgcatc
    32221 ctccatcatg taggtcagca gctcgcggaa cagcccggtg tagagggtgt ccagctcgac
    32281 gtcgttggcg cgcaggcgca gtgcggcgtc gccgtcgcgt tgcacgtagg cgtcgagcac
    32341 gtcgcgcacc tggcgcgcgg ccagccggcc cagcgcgtcc agcccgcggg tatgcggcag
    32401 cggcggggtg aggttgagcg ccatcgagcg cttggcgatg ttcgccgcga ggtcgccgat
    32461 gcgctcgatg tcggaggcaa cccgcaacgc ggccaggatc acccgcaggt cgccggccag
    32521 cgggccgcgc cggatcagcc ggatcacgtc gtggttgacc tgctgctcga gcgcgtcgat
    32581 cgcctcgtcg ttgccgatca cccgctcggc ggccttgtcg tcgcgccgct cgaccacgtc
    32641 cagcgcggcc tccagctgcg ccaccgacat ctggcccatg cggatcagct cgtcgagcag
    32701 ggcctgctgc tcctggtcgt agctcttgac gatgtggttg tgctcgttca tcagccgaac
    32761 ctgcccgtga tgtagtcctc ggtctgctgc ttcgagggat tggagaagat ctgctcggtg
    32821 gcgccgtgct cgatgaggtc gcccaggtac atgaaggcgg tgtagtcgga gacgcgcgcg
    32881 gcctgctgca tgttgtgggt cacgatcatg atcgtgaagt cctgcttgag ttcctcgacc
    32941 agttgctcga tgcggctggt ggagatcggg tccagcgccg aggtcggttc gtccagcagc
    33001 agcacttccg gcttcagcgc caccgcgcgg gcgatgcaca ggcgctgctg ctggccgccg
    33061 gacaggccca ggccgctgtc cttcagcttg tccttgacct cgtcccacag cgcggcctgg
    33121 cgcagcgcca gctcgacgcg cgcgtccatt tcggccttgc ccaggcgttc gtggtggcgg
    33181 atgccgtagg cgacgttctc gtagatcgtc atcgggaacg gcaccggctt ctggaacacc
    33241 atgccgacct tgctgcgcag ccggttcatc gggtacttcg ggtcgaggat gttctcgccg
    33301 tccagcatca cctcgccctt cgccaccagc ttgggataca gcgcgtagat ccggttgaac
    33361 acccgcagca gggtcgactt gccgcaaccg gacggcccga tcagcgcggt cacccgcttc
    33421 tccgggacgt ccagcctgat gtccttgacc gcgtggaact tgtcgtacca gaagtccagg
    33481 ttgcgcgcgc tgatcttcac cggcggcgcc agcgtcgggg tgtcgcgcgg gtgtgcgacg
    33541 gcgaggtgca aatcgttcat gtcggggatc cgcgagaaat cagtcatggg tcatcttgtt
    33601 gcgcgatacg atcgcccggg ccacaaggct ggtcagcagc acgaacaggg tcaggacgaa
    33661 ggcgccggcc caggccagcg aatgccagtt ctcgaacggg ctcatcgcat actggaagat
    33721 caccaccggc acgttcgcca tcggcgacag gacgtcggtg gtccagtact ggttgttgag
    33781 cgcgctgaac agcagtggcg cggtttcgcc ggagatccgc gcaagcgcca gcaggatgcc
    33841 ggtgacgatg ccgggcaggg cgctgcggta cagcacctgc atggtcacct tccactgggg
    33901 cacgcccagc gacagcgccg cctcgcgcat ctgcgagggc accagccgca gcatctcgtc
    33961 ggtggtgcgc accaccaccg gcagcacgat gaaggccagc gcgatgccgc cggcgagcgc
    34021 cgagaaatgc cccatctgcg ccaccaccat ggtgtacacg aacaggccga gcacgatcga
    34081 gggcgccgac agcaggatgt cgttgacgaa gcgcacggtc tcgccgagcc agctcttgct
    34141 ggagtattcg gcgaggtagg tgccggccag caccccgatc ggcgcgccca gcaggatccc
    34201 gagcatgctc atcagcgcac tgccgaagaa cgcgttgagc atgccgccgc cctcggcgcc
    34261 cggtggcggg gtcatttccg tgaacagcgc caggttcagc gaactgacgc ccatccgcag
    34321 ggtggtccag atgatccaga ccagccagaa gatcccgaac accgcggcca gcaccgccag
    34381 cacctgggcg gcgatgttct tggcggcgcg gatggcatag agcggcgtgg ccatcagttg
    34441 ccctcccgct tgttgaggct gcgcagcatc agccgggcga tgctgagcac gatgaaggtg
    34501 acgatgaaca gcacgaagcc gagcgcgatc agcgaggagc ggtacatctc cgaatcggct
    34561 tcggtgaatt cgttggcgat ggtcgcggcg atcgagttgc cgggttccag cagcgacatg
    34621 gtcagcgaat gcgcgttgcc gagcacgaag gtgaccgcca tggtctcgcc cagcgcgcgg
    34681 ccgaggccca ggaagatgcc gccgatcacc gccgaacgcg tgtacggcag gaccacgtcc
    34741 cacaccactt cccagcgggt cgcgccgagc gcgtacgccg attccttcag cctgccgggc
    34801 acggtcagga acacctcgcg catcaccgac gacacgaacg ggatcaccat gatcgccagc
    34861 accaggccgg cagtgcccat gcccagcccc agcggcgggc cggcgaacag cacgccgacg
    34921 aacgggattt ggccgaagtt cgcatccacc cacgggtaca cgtgttcggc cagcaccggc
    34981 accagcacga acaggcccca catgccgtag atgatcgagg gaatgcccgc cagcagctcg
    35041 atcgccgcgc cgaccggggt gcgcaaccac ttcggcgcga tctcggtcag gaagaaggcg
    35101 atgccgaagc tgatcgggac cgcgatcagc atcgcgatga aggcggtgac cagggtgccg
    35161 tagatcggca ccagcgcgcc gaactgctgc tgcaccgcgt cccactcgtc ggtccacagg
    35221 aaggcgatgc cgaacttctg gaaggccgcg cggccgcccc acagcatcga cagcgcggcg
    35281 gcggcgagcg acaccagcac gaacacgccg gcaccggtca ccagccagcg gaagcggttg
    35341 tcggtgcgga tgtcggcgag gtcgcgggaa tcgtctgcgg cggtcggggc aatggcgttc
    35401 atggtgttcg cggtgtcatc cggtcgtgtc gagccggcgc cacgggcgtg gcgccggtgc
    35461 gcgcggcgtg gtcgtcaggc cttgatgtcc gatgcccagt aggcctcgat ctgctgcacc
    35521 agttccgccg gcagcggcac gaagtgcagg gcgtccgcct gcgcctggcc ctgctcgaac
    35581 gcccacttga agaagtccag ggtgtcgcgg gtgcgcttgg cgtccttcgg ctgcttgtag
    35641 accaggatga agttggtggc ggtgatcggc cacgcgtccg cacccggggc atcggtgacg
    35701 accaggctga agtcccgggc gttcttccag tcggcgctgg cggcggccgc ggcgaacgag
    35761 gccgccgatg gctgcaccca attgccggcc gcgttctgca tgccggcata ggccatcctg
    35821 ttctcggtgg cgtaggccag ctcgacatag ccgatgctgc ccttgatctg cttcacgtag
    35881 gcagccacgc cctcgttgcc cttgccgccg atgccgccgg gccagttgac cgaggtgcct
    35941 tcgccgaccg tggccttcca cgccgggctg accttggaca ggtaattgac gaaattgaag
    36001 gtggtgccgg aaccgtccga gcggtgcacc acgttgatct tggaggaggg cagcggcagg
    36061 ccgggattga gcgcggcgat cgccgggtcg ttccaggtgg cgaccttgcc gaggaagatg
    36121 tcggccagca ccgcgccggt caggcgcagc ttgccggctt ccaggccctc gaggttgacc
    36181 accggcacca ccccgccgac cgcggacggg aactgggcca ggccgtcctt cgccagttct
    36241 tccggcgcca gcggcttgtc ggaggagccg aagtccacgg tgccggcctt gatctgggcg
    36301 atgccgccgc cggagccgat cgactggtag ttgatcctgg ccccggtggc cttgttgtag
    36361 tcgtccgacc acttcgaaag cagcggatag atgaaggtgg cgccggcacc cgtgatttcc
    36421 accgatttgc ggtcgccggc cgttgccggc gcagcggtcg gcgccgccgc cggatcggcg
    36481 ctggcggcgg ccgcggcggg ctcctgcttg caggcggcaa ggccggccag caggcacagg
    36541 ctgagggcgg cgaggcgggg cagttggcgg ctcatgtggg ggtcttccag tcgcgcgttt
    36601 cggatcgcgc catgaaatga tgtttttgtg acatgtcgat gacagctgcc ccggtttcgc
    36661 ccggaattgg cggggtgcgc aagaaaaagg cgggcccgaa ggcccgcccg aacgcacacc
    36721 ctggtcaggg ggattggcgg gatcagaact tgtagttctg cgcccagtac gcctcgacct
    36781 gctggaccag ttcgtccggc agcggcacgt agtccagcga gcgggcctgc tcgtcgccat
    36841 tggcgtaggc ccagcggaag aactccagcg cctgcttggc gcgcgcggca tccttcggct
    36901 tcttgtgcat caggatgaag ttggtggcgg cgaccggcca ggagttgtcg cccggcgcgt
    36961 tggtcatcac caggaagaag tccttcgagt tcttccagtc ggcgctggcg gcggccgcgg
    37021 cgaagctcgc atcgctcggg ttcacgtagt tgccggcggc gttcttcatg cggctgtagg
    37081 ccatcttgtt ctgcagcgca taggacagct cgacgtagcc gatgccgccc ttgatctgct
    37141 tcacgtacgc ggcgacgccc tcgttgccct tgccgccgac gccggccggc cacttgaccg
    37201 aggtgccttc gccgaccttg gccttccact ccgggctgac cttggacagg tagttgaccc
    37261 agttgaaggt ggtgccggaa ccgtccgaac ggtggaccac ggtgatcttc atgtccggca
    37321 ggttgacgcc gccgttcagg gcgacgatgc gcggatcgtt ccacttggcg accttgccga
    37381 ggaagatgtc ggcgaccagt tcgccgtcca gcttcatcgc accggccttc aggcccggga
    37441 tattgatcac cggcaccacg ccgccgatcg ccgacgggaa ctggcccagg cccgcggcct
    37501 tcagttcctc gggcggcagc ggcttgtcgg aggagccgaa gtcaaccgtc ttcgccttga
    37561 tctgggcgat gccgccgccg gagccgatcg actggtagtt gatcttgttg ccggtggcct
    37621 gcgagtagtc ggccgaccac ttcgagagca gcggatagat gaaggtggcg ccggcaccgg
    37681 tgatgtcggc ggcggcggcg gtgaacacga agccgccggc gagcacgagg gctgcggtgc
    37741 ggtgcttgag gtagttggac acgcggtact cctgggtggc tgatgcgcgg actcgccgct
    37801 gccgcgcatt ccactgcggt tggatgacag aagcgtgttg tcgaagtgac cgtttgatga
    37861 cagcgctggc gcgccctgcg tgtcccgtga cacatgggcc gcggcgcgga ttcatcgaac
    37921 tgtcatcttt cgacaacacg actgtcagaa tccggtcgca ggctgtgccc gtttcacgga
    37981 gtcttaacgg ccgcccggcc gcaggcaccc aacctacttt cgatcctttc cggagcatga
    38041 ccatgcaacg caagaccctc gccctcgccg tgaccatggc gctggccgcc accagcttcc
    38101 aggccagtgc ccaggatgcg cgcgaccagg aactcgccgc gctcaagcag caactggcgg
    38161 agctcgccgc caaggtgcag gagctcgagg accgcagcga cgcgcagtcc gacgtcaacg
    38221 tcgataccgc gacccagctc gacaccctgg ccaacaacag caccaaggtc gagaccaagg
    38281 gcggcatcaa ggtcacctcg gccgacaaga acttcgaggc ctcgatcggt ggccgcgtcc
    38341 atttcgatgc ctacgcgttc gacaaggacg tggccgcgac caccgacacc accgagttcc
    38401 gccgcgcccg cctgaccctg tccggcaagg cctacggttg ggaatacaag gtcgaggaag
    38461 atttctccgg cggcagcaac ctggacggca tccgcgacct ctacatcgcc cgcaacatga
    38521 tgggcggcaa ggtcaccatc ggccacttca agccgtaccg cgcgctggag gagctgacca
    38581 gttccaacga aatcctgatg atggagcgcc ccagcaccac cgccaccggc atctacaacg
    38641 gccgccagtt ccagcagggc gtgggctacc tgcgcgccgg cgccaccggc aactacacct
    38701 tcggcgtcag cgcgttcaac atgcgcagcg cgccgactgc gcgcaacgag ggcgtgggct
    38761 tcgccggccg cgccacctgg gcgccgatca acgacggcac caccgccttc caccttggcg
    38821 cctcttacag cttcgagaac ctgaacaagg gcaccccgac cgtgaccgcg gtgtcgcact
    38881 acgccggtcg ccgcggcccg tcgcagacca tggccaccac caccgccgcg gtcggcacca
    38941 acgactccca ggtcagcacc tatggcctcg aggcggcgta cctcagcggc ccgttcttcg
    39001 tgcagtccga atatgcgcgc ggcaactacg gccaggccag cggcggctcg caggacgtgg
    39061 acgcgttcta cgtgcagggc agctggatgc tcaacggcgg gatgaaggcg tacaagccgg
    39121 gcaccggcgt gttcggctcg ccgaaggtcg ccgacaaggg cctgtgggag ctcaccgcgc
    39181 gttgggacac gatggagaac aacgacctgc tggacaccaa gaccacctcc accgtccttg
    39241 gcgtgaacta ctacgtcaac ccgaacatgc ggatcatgct gaactggacc cagggcgaca
    39301 acgacaagac cggcgacgag ccgagccagc tcgcgctgcg cacccagttc gcgttctgac
    39361 ccgggcgcac ctcgtgcaac accggacgcc ccgcttcgtg cggggcgtct tcatttgcgg
    39421 ccagccgcga gggccagcgc cgtgcatcgc ccggcaacgc gcgggccctc gttcagggca
    39481 gcagcaccgg cccggccgac gccgccttgt ccggccaccg gcacaggtcg cggaccgggc
    39541 agcgcgggca ctgtggcttg cgcgcggtgc aggtgtagcg gccgtgcagg atcagccagt
    39601 ggtgtgcgtc cagccggaat tccgccggga ccagcttcac cagcttgtcc tcgacgatgc
    39661 gcacgttggt gcctggcgcc agcccggtgc ggttggagac ccggaagata tgggtgtcga
    39721 ccgcgatcgc cggttcgccg aacgcggtat tgaggaccac gttggcggtc ttgcggccaa
    39781 ccccgggcag cgcttccagt ccggcacggg tgcgcggcac ctcgccgccg tgcgcgtcga
    39841 gcagcaggcg gcacatggcg atgacgtttg cggccttggc attgaacagg ccgatggtcg
    39901 cgatgtatcg cttcaggccg tcctcgccca gcgcaaggat cgcggctggg gtgtttgcac
    39961 gagggaacag cttgcgcgtc gccttgttga cgcccacgtc ggtggcctgc gccgacagga
    40021 tcaccgcgac cagcagctcg aacgggctgg cgtactcgag ttcggtggtc ggcttcgggt
    40081 tcagtgcacg caggcgggcg aacaggtcgg cgatctcctc gcgggtcagc ctgctgccgc
    40141 gcagggggcg gcgcctgccg gcgaccacgg tcatcgcggc ccgcgccgcg cggccttcgc
    40201 cagcgcacgc gccagcacgt cggcagccgc ggccggcagc gcgggtgcgg gcgctgcggt
    40261 ggacgcgggg acggggcgtc gcgcggcctc gcggccggca gcgcgccgca gcaggcgttc
    40321 cgcgcgcgcg cggtggcgtt cgcgcgcggc cagcgcggag tggcgtgcat cgcgcgcctc
    40381 gatcaggcgc acgttgcagg cggcatcgca gccggggcag ggcagggcat cgagcagtcc
    40441 gtgctccatc gccgcatcca ggtcgtcgat ggccagcatc gccagcagtg cgtgcgctgg
    40501 atgcgcgcct gcgcagccgc agtgctggca ggcaggcgcc atcagcggcc gttgaacgcc
    40561 ggcttgcgct tttcgaggaa ggcggcggtg ccttcgcgca tgtcgtcggt cgagaacacc
    40621 aggccgaatt gcgccgactc gtactccagg ccttcgccga tcccgcattc gccgccgatg
    40681 ttcacgcaat cgagcatccc gcgcagcgcc agcggcgcgg ccgccgcaag ttgggtggcc
    40741 agccgcatgg tttcggcttc caggtccgcg gccggcacca cgcggttgac gatgccgagc
    40801 tgcagcgcgc gttcggcgct gatgggcgcg ccggtcaggc acagctccag ggtcgccgca
    40861 cggccgcaca ggcgcagcag gcgctgggtg ccaccgaacc cggggatcag gccgaggttg
    40921 atctccggct ggccgacctt cgcgctgtcg gcggcgatcc gcaggtggca gcacatcgcc
    40981 agctccagcc cgccacccag cgcaaagccg ttcaccattg cgatcaccgg cttgggcagc
    41041 gtttccaccc ggcgcatcat gcgggtgccg cgcagggaga aatcgcggcc ctggacgggg
    41101 gtcagggcat tcatctcggc gatgtcggcg ccggcgacga aggccttcgg cccgctgccg
    41161 gtgagcacca ccacgcgcac ggcggcgtcg gcggcggcgt cggcgaaggc tacgtccagt
    41221 gcgtccaggg tggcggcgtt gagggcgttg agcttgtccg ggcggttgac gcggatcgtg
    41281 cgcacgccat cggcagtgtc gatcaacagc agggggtcgg tcatgcggcc tcgcggtggg
    41341 tggggtttcg ggaaggacgt ggatggctgg aacttccgcc gccacgccgt gtcggaggag
    41401 gggcctcagt ggcggttaag cgctgcggcc gctatcctag cgtgtcaatc cggggcagtt
    41461 cgaccggcga gatgtcaaca ccgcgcggcc atcggtcgcg gcggacccca gggcggcacg
    41521 gccgccccaa attttgagcg aggttgtaaa tgaagtttcg cgcgcttgct gccagtgtgg
    41581 cggccctgac cctgaccgcc ggcatcgcct cggcgcagac caagccggcc gcgccggcac
    41641 cggctcctgc caagcctgcg gccgctccgg ccaagcccgc ggctccggtc gtggacaaga
    41701 cccatgccag ctacgccttc ggttggacgc tcggccagga cctggtgaat tcgggtgagc
    41761 tggtcgacat cgccaccgtc atccgtggtc tgcaggatgc ctatgccaag aagcagccgg
    41821 cctacaccca ggaacagctt ggcgctgcgt attcgggttt ccagcagcgg gtgatgcaga
    41881 agcgtgaagc cgagttccgc aagctggcgt ccgacaacga ggcgcagtcc gccggcttcg
    41941 ttaccaagtt caaggaccag accggcgtca tcaccctgcc gagcggcatc atgtaccggg
    42001 tgctgcgccc gggcaccggt gccaagccca ccgccaccag ccaggtcgag atcgtgttcc
    42061 agacgttcct cgcggcggtc aacgtgccgc tgagcggcga gcagaccccg gcgccgttca
    42121 aggttgccga cgcgccgatc ccggcgctca aggaaaccct tccgctgatg cagcagggca
    42181 ccgtgtggga gatggtgatc ccgccgggca agctgggtgc ggacaatccg cagttcgcca
    42241 agcaggccgt caccatccgc gcggcgctga gcggggtgaa atgatgcttt gcggcgggtc
    42301 cgcatgagga cccgccacgg ccggcccgcc ccgcaccgtc ggggttggcc tggtcgatgg
    42361 gctttcgatg cgccggtcag tccggcgctt cgttttttgc aggcaacgcc acggtgatgc
    42421 catgacgatc cagtatcgcg ccgtgctcag tccctgcatc ggcatctgcg agatcgccga
    42481 cgacggcttg tgcagcggct gccaccggac gggcgacgaa gtggcggcct ggccgctgct
    42541 gaacgacgac gtgcgcctgc acctgatgga cgaggtgttg ccgcggcgcg cagccgtgcg
    42601 cgggcaggcg tgatgcagtc ggccgcgctt gccgaatcgc tgcggcgggc cacgcatccg
    42661 ttggcgttgc cgccggcggg cctgccctgg aacctgcagg aactcgacgg cctgctggcc
    42721 ccgggcgagc cgcgtcaggc cgcggtgctg gtgggcgtgg tcgcgcatgc cggggcgccg
    42781 acggtgctgc tgacccgccg ccacgacggc ctgcgccacc acgcgggcca ggtgagcttc
    42841 cccggtgggc ggatcgaggc gttcgatgcc agcccggccg ccgcggcgct gcgcgaggcc
    42901 agcgaggaag tcggcctgca ggcccgccag gccaccgcgc ttggctatct cgacccgctg
    42961 ctcaccatca ccggcttccg ggtggtgccg ctggtggcca tggtggagcc gggattcgtg
    43021 ccgcggccgg atccgggcga ggtggccgag gccttcgagg tgccgctgga cctgctgctg
    43081 gacccggcga tgctggagga aatcgagctg cacttcggcg gacggccgcg tcacgtgctc
    43141 caataccgtt atgaacgaca gcggatctgg ggcgtgaccg cctcgatcct gttcaacctc
    43201 cgcgaacggc tcgcgtccgc aacccccggg agggactgat ggccggatgg acaaccctgg
    43261 tgcaggccga aaccctggcc gtcgccctca atcgaccgga cctggtgatc gtggattgcc
    43321 ggttcagcct gctcgacccg aatgctggcg agcatgcgta tggcaccacg cacctgccgg
    43381 gtgcggccta cgcgcacctg gaacgcgacc tttccgacat gtcgccgggc ggcgaaggcc
    43441 gccatccgtg gccgcccgcc gacatgttca ccgccaggct ggcgcgctac ggcatcgagc
    43501 ccgggcacca ggtggtggcc tatgacgatg gcgacggcgc gatcgccgcg cgcctgtggt
    43561 tcctgctgaa gatgcttggc cacgagaagg tggccgtgct cgacggcggc tgggcgcgtt
    43621 ggaccgcgct tggcctgccg gtgtcgtcgg cgccgcgggc gcccgccgtt tcggcttacc
    43681 gcgcggctta cgaccactcg cgcctgttcg acgcgaatgc gctgcaggcg cacctggaca
    43741 acggcggcat gctggtggat gcgcgcgcgg cggcccgctt ccgcggcgac gaggaaccgc
    43801 tggaccggat tgccggccat gtgcgcggtg cggtcaaccg gccgtacgcg ctgaacctgg
    43861 ttgacggccg cttcaagccg ccgatgcagc ttgccgacga ataccgcgcc ctgctgggca
    43921 cgcactcgcc ggaccaggtg gtggtgatgt gcggatccgg cgtcaccgcc tgccacaacc
    43981 tgctggcgat ggaacgcgcc ggcctgcgcg gcggcatcct ctacaccggc tcctggagcg
    44041 gctggatcag cgatccgtcg cggccggtcg caaccggcgg ctaagcgatc cccgtccggc
    44101 gcgcgcagcc ggccggcgcg gtccctactt gccgatccgc gccagcatcg cgcgcagcgc
    44161 cgcctgggtg tccggctgca tccatccggc gacgaagcgg ggcaggtcga tgcgttcgtc
    44221 gcgcaggcag gcggccacgt cggcacgggc gatccgccgg gtttccagca tcggcccctg
    44281 cggcaactgc agcagctctt gcagccacac gcgggcgcgg gtggcgacat cgtcgatgcc
    44341 ggtgacttcg tcgaccagcc cgatccgcag ggcctgcgcg gactccacca tcgcaccggc
    44401 caccagcagt cgctcggcgc ggtgcgcgcc gaccgcccgc gccatcagcg cctggatgcc
    44461 ctccggcgcc accagcccga cctgggtctc gttgaggccg atccggtacg ggccatcggc
    44521 catgatccgg tagtcgcagc acagcgccag cacgcagccg cccgccggcg catgcccggc
    44581 catcgccgcc accaccggca ccgggcagct ggccagcgcg cgcgcggcat cgaagaacgc
    44641 ggtccacgcc tgcagcagcg cggcctggtc gtcgcccagc gagagcagga acggcacgtc
    44701 cagcccggcc gagaacacct tggggccacc cgcgagcacg atcccgcgcg cgccgttcgc
    44761 cagtgcgccg gccagcgacc cgctgatggc ccggcacagg tccggattga gcgcattgac
    44821 cggtgcgcgt gccagccgca actcggtgac gtggccgtgg acaagggttt cgacgaggga
    44881 tccgttcggc gacatggcgc tgggttccgg tgcgggacag ccggtatcat aggcgcatga
    44941 agatcatcct cgcgttgctg ctgtcgttgc tgccgctgtc cgcctgcgcg cgcgattgcg
    45001 ccccgcaggt caaggacggc tggatccgcc tgatgccggc cggcatgccc atgcacgccg
    45061 ggttcggccg catcgagaac cgctgcccga tgccggcgtc catcgtcgcc gcctccagtc
    45121 ccgcctacgg cagcgtggag ctgcacgagt cgaagctggt cgacggcatc agccgcatgc
    45181 gccaggtgcc ggagctgcgg atcgcgccgg acggcgcggc ggtgctgcaa ccgggcggcc
    45241 tgcacctgat gctgatgcag ccgaaggcga cgctgaagcc gggcagccgc gtcgcgatcg
    45301 aattcgagct caaggacggg cgcaagctgc ttggcgaact cgaggtccgc aagccggcgc
    45361 cctgatccgg ggattggcgg cggcacgact ggccgcaagg cgccttcttg gcgcgccggt
    45421 gcggcgaagc ctcagccctg cagcagcgct tccgatgcgc gccgcacgcc atccggcagc
    45481 ggcgtcggcc tgccgctgcg gcggtcgatc cacaccgcga ccacgctgcc gtcgcaatgc
    45541 aggccgccgt cggcatcgac gatgcggtgg ccgatcacca cgctgctggt gccaagtttc
    45601 gcggtgaaca gttcgacgaa gatgctggcc gggtactcga tcgggcgctt gaagttgagc
    45661 gtcgccgacg ccaccaccgg cgccgtctcc gtggtgaccc acggctcggc gatggtgtcg
    45721 aaccactcga tgcgcgcctg ctcgatgaag gtcaggtagc gcgcattgtt gacgtggttg
    45781 aaggcgtcga ggtcgctcca gcgcagtgcg accggcctgc ggaacaggtt gttcatggtg
    45841 cacggctcat gtggtcttgt tcctggcctt gcgcggcgca cccttcggcg ccggcttcga
    45901 gcctgcacga ttgcgttcga gcaaaggcgc aaggaagcgg ccggtgtgcg aggccgggtt
    45961 ggcggccacg tcttccggcg ttccggtggc gatgatctgg ccgccgcgat ggccgccttc
    46021 ggggccgagg tcgatcaccc agtccgcggt cttgatcacg tccaggttgt gctcgatgac
    46081 gacaatcgtg ttgccgtcgt cgcgcagctt gtggagcacc gccagcaggt gctcgatgtc
    46141 gtggaagtgc aggccggtgg tgggttcgtc gaggatgtag agcgtgcgcc cggtgtcgcg
    46201 ccggctgagc tccttcgaca gcttcacccg ctgcgcctcg ccgccggaca gggtggtcgc
    46261 cgactggccg agcttgatgt agctcaggcc cacgtccatc agggtttcca gcttgcgggc
    46321 gatgctcggc accggctgga acagctccag tgcatcctcc accgtcatct ccagcacgtc
    46381 gcggatgctg tagcccttgt acaggatctc cagggtctcg cggttgtagc gcttgcccgc
    46441 gcagacgtcg cagggcacgt acacgtccgg caggaagtgc atctcgacct tgatcaggcc
    46501 gtcgccctgg caggcctcgc agcgcccgcc gcgcacgttg aagctgaagc ggcccggcgc
    46561 gtagccgcgc gcgcgcgctt ccggaacctg cgcgaacagc tcgcgcagcg gggtgaacag
    46621 accggtgtag gtcgccgggt tggagcgcgg ggtgcgcccg atcggcgact ggtcgatgtc
    46681 cacgaccttg tcgaacaggt cgatgccgcc ggcttcgcga tacggcgccg gggtgtgcga
    46741 ggcgccgttg atctcgttgg cggccagcgc atacagggtg tcgttgatca gggtcgactt
    46801 gccggagccg gagacgccgg tgatcgcggt gaacagaccg gaggggatct ccaggtcgac
    46861 gtccttcagg ttgttgccgc acgcgccctt cagcgcgagc atcagcttgc ggttgggcgg
    46921 atgccggtgc gccggcaccc cgatcgcctt gcgcccggac aggtactgcc cggtcagcga
    46981 ccgcggcgcc tgcagcacgc tctccagcga gccttgcgcg accacctcgc cgccgtgcac
    47041 gccggcgccg gggccgatgt cgacgatatg gtcggccagc cggatcgcat cctcgtcgtg
    47101 ctcgaccacg atcacggtgt tgccgaggtc gcgcaggcgg gtgagggtgc cgagcaggcg
    47161 ctcgttgtcg cgctggtgca ggccgatcga tggttcgtcc agcacgtaca tcaccccgac
    47221 caggccggcg ccgatctgcg atgccagccg gatgcgctgg gcctcgccgc cggacagcga
    47281 atccgccttg cgctccaggg tcaggtaatc cagcccgacg tcgaccagga agccgagccg
    47341 ttcgcggatc tccttgacga tcctggccgc gatctcgccg cgccagccgg gcagggccag
    47401 gccctcgaag aacgccagcg aggcgtcgat cggcagcatc gcgacctccg gcagcggctt
    47461 gtcggcgacg aacacgttgc gcgcggcgcg gttcaggcgc gcgccgccgc attcggtgca
    47521 ggggcgctcg ctgatgtact tcgccagttc ctcgcgcact gccgccgatt cggtctcgcg
    47581 gtagcggcgc tcgaggttcg gcaggatgcc ttcgaagctg tgcttgcgct ggtacttgtt
    47641 gccgttctcg ccgacgtaga cgaggttgat cgcctcgctg ccgctgccgt gcaggacgat
    47701 gtcgcggacc tgcttcggca gggtctgcca gaccgcatcg acatcgaaac ggtagtgctt
    47761 cgccagcgac gtgatcatcg agaagtagta ggcgttgcgc cggtcccagc cgcgcatcgc
    47821 gccggccgcc agcgacagtt ccgggtgcgc caccacccgc gccgggtcga agaactgcga
    47881 cacgccgagg ccgtcgcagg tggggcaggc gcccaccggc gagttgaacg agaacagccg
    47941 cggctccagc tccggcagcg agtagtcgca gaccgggcac gcgtacttcg aggagaacag
    48001 caacggctcg cggtcggccg cggtcgcttc cggcgtcctg cctgcagcga cacttgcgct
    48061 gtcctgcgcg gcggtcgctt ccggcgtcct gcctgcagcg acacttgcgc tgtcctgcgc
    48121 ggcgtccatc gactgcacga tcgccatgcc gtcgccgagc ttgagcgcgg tctcgaacga
    48181 ttccgccagc cgctggcgga tctccgcgcg cggcttgaag cggtcgatca ccgcctcgat
    48241 ggtgtgcttc tggcgcaaac ccagcgccgg caccgcgtcg atctcgtgca cgtggccgtt
    48301 gacgcgcacc cgcgtgtagc cctgcgcgcg cagttgctcg aacacctgca cgtgttcgcc
    48361 cttgcgctcg cgcaccaccg gcgccagcag catccagcgc tgttcggcca gcgccggctc
    48421 ggcctccatc gccagcacct ggtcgaccat ctggctgacg gtctgcgctt ccagcgggaa
    48481 gtggtggtcg gggcagcgcg ggctgcccac ccgcgcgtac agcaggcgca ggtagtcgtg
    48541 gatctcggtg atggtgccga cggtggagcg cgggttgtgg ctggtcgatt tctgttcgat
    48601 cgagatcgcc ggcgacagtc cctcgatgtg gtcgacgtcc ggcttgtcca tcacgctcag
    48661 gaactgccgc gcataggacg acagcgattc gacgtagcgg cgctggccct ccgcgtagat
    48721 cgtgtcgaac gccagcgagg acttgccgga cccggacagc ccggtgatca cgatcagctt
    48781 ctcgcgcggc aggtcgaggt cgatgttctt gaggttgtgc gtccgtgcgc cgcggatgcg
    48841 gatgaagtcc agcgccatcg tgggtctgtc ctgcgtgggg ggatggcagg cggatgcccc
    48901 gcaggccaat cgggaagcgt agcgagccac ctgtttgggg gcaaatggcg gaattgccag
    48961 ttcccggaac ccggcgaggg gcctgcagtt gaccctaacc cgctgaatcc cctacaattc
    49021 cgctcctgtc tgccctcgat ggcaggcagc gtgtcgggag caacggccgc cacgaccaag
    49081 aagaactaca gaaaccacac gtccaggaaa cacaatcatg tacgcagttt tggtcaccgg
    49141 cggtaagcaa taccgcgtga tgcagggcga aacgctccgc gtcgagaaac tcgaagccga
    49201 agccggcagc gagatcaagt tcgacaacat cctgctgctc ggcgacgccg acggcatcaa
    49261 gctgggcgat gcgctgaagg gcgccaccgt cgccgccaag atcgtcggcc acggccgcgc
    49321 cgacaaggtg cgcatcgtca agttccgccg ccgcaagcac catcgcaagc agatgggcca
    49381 ccggcagcat tacaccgaaa tcgagatcac cgggatcgct ggttccggcg acaacaagta
    49441 aggagacgca gtcatggcac acaagaaggc cggtggttca tcgcgcaacg gccgcgactc
    49501 caacccgaag tacctgggcg tcaagatcta cggtggccag gcgatcgaag ccggcaacat
    49561 catcgtccgc cagcgtggca cccagttcca cccgggcatg ggcgtcggcc tcggccgcga
    49621 ccacaccctg ttcgcgctgg tcgatggcaa ggtcgagttc gcgaccaagg gcccgaagaa
    49681 gcgccgcatg gtcagcatcg tcgccgcgga gtaaccggct cccggcgcac ctgcgaacgc
    49741 ccgtccaggg atggccgggc aggacgaccc gaagtcgccg tggcgacgcc agggatggca
    49801 acgacaaggc cccgccaagg cggggctttt cgtatctgag agaatcaatc ccatgaaact
    49861 ggtcgacgaa gccgaaatca ccgccaccgc gggcaatggc ggcaatggct gcatcgcgtt
    49921 ccgccgcgag aaattcatcc ccctcggtgg ccccaacggc ggcgatggcg gcgatggcgg
    49981 caatgtctgg ctgcaggccg acgagaacct caacaccctg gtcgacttcc gccacgagac
    50041 ccggttcaag gcccagcgcg gcgagaacgg catgggcagc cagatgtacg gcaaggccgg
    50101 cgaggaccgc acgatcctgg tgccggtcgg cacggtggtg cacaacgtcg acaccgacga
    50161 ggtcatcggc gacctgaccg cgcatggcca gcgcctgctg gtggcgaagg gcggcaaggg
    50221 cggcctcggc aacatgcatt tcaagagttc ggtcaaccgc accccgcgac gggcgacccc
    50281 gggcaccgag ggcgagaccc gcaccctgcg gctggagttg aagctgctgg cggacgtcgg
    50341 cctgctgggc ttcccgaacg ccggcaagag cacgttcgtc cgcgcggttt cggcggcgac
    50401 gccgaaggtc gcggactatc cgttcaccac gctgtatccg aatcttggcg tggtcagcgt
    50461 ggagccgggc cgcagcttcg tgatcgccga catcccgggg ctgatcgagg gcgcggccga
    50521 cggcgccggg ctcggcagcc tgttcctgcg ccacgtccag cgcacccgcc tgctgctgca
    50581 cctggtggac atcgcgccga tggattacga gggcagttcg ctgtcgccgg ccggacaggt
    50641 gcgcgcgatc gagaacgaac tgcgcaagta cgacccggcg atgctggaga agccgcgctg
    50701 gctggtgctg aacaaggccg acctgatgtt cgaggacgag gcgcaggccg cggccgaagc
    50761 gatcgtcgcc gagctggatt ggaaggcgcc gtggtacatc acctcggcga tcagccgcga
    50821 aggcacccgc ccgatcatgc tggcggtgca ggccttcttc gaccgcctgc gcgaggacga
    50881 actggaagcg gccgcgaacg ccgccgcggg cgcgggcagc gatgacgccg gctgagcctg
    50941 cggcgtcgac agggaacagc ccgctgcgct gcgcccgctc cgtttgccgg gaccgcgcgg
    51001 caggtcgcat gctgcagctt cggcaggcaa caaaaaaccc ggcttgcgcc gggtttttcg
    51061 cgacagccgg cgaacgctta cgcggccttc agcgccttga tgcgcgcggt caggcgggcc
    51121 ttgtggcggg ccgccttgtt cttgtggatc aggccgcggg cggcgaaccg gtccagcagc
    51181 ggctgcgcgg tgacgaatgc agcggtggcg gcttcggcat cgttggcgtc gagcgccttg
    51241 atcaccttct tgacggcggt acgcagcatg gagcgctggg cgacgttgcg ttcgttgcgc
    51301 acgacggtct gcttggcgcg cttcttggcg gacttgatat tggccacggg gaaaatcctg
    51361 ggaagtcggt tggggctggg agaatcggcc gccggcagga gtgcccacgg acgaaaggat
    51421 cgggaagact caaaattatg gacgattcaa tggcttgggt caacatgccc ggtgccgcag
    51481 tggcccgccc ggcggcgcgc gcatgagcgg cctgttgcgt tccagcgcag tgttcagcgc
    51541 gatgaccctg ctctcgcgga tcgccggctt cgtccgcgac atgctgcagg gcatgctgtt
    51601 cggcaccggc ggggcgatga gcgccttcat cgtggcctac cggatcccca acttcctgcg
    51661 ccgggtgttc gccgagggct cgatggcgat ggcgttcgtg ccggtgctga acgagatcag
    51721 gcagaagggc gacaaggccg cgctgaagga cttcatcgat cacatggccg gcgcgctttg
    51781 cgcggtggtg ctggtggtgg ccgccgccgg cgtgctgctg gcgccagtgg tggcgcagtt
    51841 gttcgctccg ggctggagtg gcgaggaggc ggtgaagctg ggtcagaccg cggcgatgct
    51901 gcgggtgacc ttcccgtacc tggtgttcat ctcgatgatg gcgctggcgg gctcggtgct
    51961 gaacagcttc ggccggttcg cgctgccggc gttcaccccg gtgctgcaca acctgaccat
    52021 gatcgcggcg atgttctggc tggcgccgcg gttcgacatc aggccgatgg ggctggcctg
    52081 gggcgtgctg gtcgccggtt tcctgcaact ggcgctgctg tggccggcgc tgggccggct
    52141 ggggctgcgc ccgcggctgc ggatggggtt cgcgcatccg gacgtgcgca aggtcggcaa
    52201 gttgatgctg ccgacgctgt tctcgtcgtc ggtggcgcag gtcaacctgc tggtgggcac
    52261 cgcgttcgcc tcgctgctgg tggatggcag ccaggactgg ctgtattact ccgatcgcct
    52321 gatcgagttc ccgctggggc tgttcggggt ggcgctgggc acggtgatcc tgccgcacct
    52381 ctcgcgccgc cacgcggatg ccgacgcggc cggctacaac gcgtcgctgg actggggcat
    52441 gcggatggcg ctgctggtcg gggtgccggc cgggctgggg ctgatgctgc tggccgagcc
    52501 gctgaccgcc accatctaca actacggcaa gttcaccgac cacgacaccc ggatgtcggc
    52561 gatcagcctg accgtgatgg cgctgggcat ccccggtttc atgctcagca aggtgctggc
    52621 gccggcgttc tatgcccgcc aggacaccaa gaccccgatg cgcgcggcga tctggacggt
    52681 ggcggtgaac gtggcgctga cggtggccat cacctggccg ctgtggcggc agggcgtggc
    52741 gggtgcgcat ggcggcattg ccgccgccac cgggttggcc gggctgttca acgcctggct
    52801 gctgtggcgc tacctgcgcc gggccggcct gcaccggccg cagccgggtt ggggacgcta
    52861 cctgctgcgg atgctgctcg cctgcctggc gatggcgctg gcggtgctgg cattgcgcgg
    52921 gtgggtgggc gaatgggtgg tgctggccga ctggcgcctg cgggtgggct ggttgctgct
    52981 ggcggtcgcc gccggcgccg tgacctacgt cgcggcgctg ctggcgatgg gcatgcgccc
    53041 gcgcgacctg cgccactgac ggccgcgcat gctgcaccgc agctatactg ccggttcgtc
    53101 atgaatcgat ttttccgcga cgccgtggga gggccgctgt gcccgcaggg cagcgtggtc
    53161 tgcatcggcg ccttcgacgg cctgcacctg ggccatcgcg cgctggtggg ccgcgcggtc
    53221 gcgcatgcgc gtgcgctggg ggtgccggcg gtggccctgg gtttcgagcc gctgccgcgc
    53281 gagttcttcg cccgcggcga acatccgccg cggctgatgc tgccgcgggc caagctggaa
    53341 ggcctgcatg cgctcggctg cgacagcgtc ggcctgctgc gcttcgacgc gaaactggca
    53401 tcgatgccgg cgcaagcgtt cgtgcgcgac gtactggtcg agcgactgtc cgcgcgcgaa
    53461 gtctgggtcg gccccggctt ccgcttcggc aacgcgcgcg gcggcgacat cgccctgctg
    53521 caggcgctgg gcgcggagca cggcttcgtc gcgcattcga tcgacccggt gcactgcagc
    53581 gatggcgagc gcgtgtccag cacgcgcatc cgtgccgcgc tggtcgaggg cgacttcgcc
    53641 cacgcagcca agctgcttgg ccggccctat gccatcgctg gccacgtcgt gcacggtcgc
    53701 cagctcgggc gcaccctcgg ctatccgacc gccaacctgc gctacggcgg caaggccccg
    53761 gcgctgcgcg ggatctacgc gagctgggtg cacggggtga ccgacgcgcc gtggccatcg
    53821 gtgtccagct tcggcacccg tcccaccgtg gacggcatcg agccgctgct ggaggcccac
    53881 ctgttcgatt tcgagggcga cctgtacggc cgccggatcg aggtcgagtt cgtcgcccgc
    53941 ctgcgcgacg aagagaaatt ccccgacctg ccgtcgctgg tggcgcagat gcaccgcgac
    54001 gacgcgcagg cccgtgccat cctgatccag acacagcagc gagccagcgc gtgaccgaag
    54061 atcgttcgaa caagaccagc gacaatccgt acaggtccac catcctgctg ccggagaccg
    54121 cgttcccgat gcgtggcgac ctgcccaggc gcgagccgga gacgctggcg cgctgggaag
    54181 cagaaggcct gtacgcacgg atccgcgggg tcgcgaaggg gcgcccgcag ttcgtgctgc
    54241 acgacggccc gccgtatgcc aacggcgcga tccacctggg ccacgcggtc aacaagatcc
    54301 tcaaggacat catcgtcaag tcgaagacgc tggccggctt cgacgcgccc tacatcccgg
    54361 gctgggactg ccacgggttg ccgatcgaga tcgcgatcga gaagaagttc ggcaaggtcg
    54421 gcgtgaagct cgacgcggtc gcgttccggc agaaatgccg cgagtacgcc agcgagcaga
    54481 tcgacgtgca gcggcgcgac ttcaagcgac tgggcgtgct cggcgactgg gaaaacccgt
    54541 accgcacgct ggatttcaag ttcgaggccg acgagatccg cgcgctggcc agggtggtgg
    54601 agaacggcca cctgctgcgc ggggtgaagc cggtctactg gtgcttcgat tgcggttcgg
    54661 cgctggccga ggcggagatc gaataccagg acaaggtctc gccggcggtc gacgtggcct
    54721 acggcgcgcg caacccgcag gcgctgagcc gcgcgttcgg cagcgaattg ccggaaggcg
    54781 tcgaggtcgc gctgccgatc tggaccacca cgccgtggac gctgccggcg tcgctggcga
    54841 tctcgatcgg cccggatctc gactacgtgc tggtcgaagg tccgctcgcc gcgaacggcg
    54901 cgcgccgttg gctggtgctg gccgaggccc tggccgaaaa ggcattgaag cgttacggcg
    54961 tcgatgaggt cgtggtgctc ggccgcgccg tcggcgaaaa actcgaaggc atgttgttcg
    55021 cgcatccgtt ctacgccgag cgcgacatcc cggtcctgct tggcgaccac gtcagcgccg
    55081 aagacggcac cggcgccgtg cacaccgcgc cgggccacgg ccaggaagac ttcgcggttt
    55141 cccagaagta cgggctggtc gagaaatatt cggcggcgca gctcaacccg gtcgatggcc
    55201 gcggcgtgta cctgccgtcg acgccgcctg ccaacggcgc cgaactcgcc ggcgtgcatg
    55261 tctggaaggc caacgacgtg ctggtggacg tgttgcgcgc aagcggccac ctgctcgcgt
    55321 tctcgaagct cacccacagc tacccgcact gctggcggca caagaccccg gtcgcgttcc
    55381 gcgccacccc gcagtggttc atctcgatgg accaggcgaa cctgcgcgac gacgcgctgg
    55441 ccgcgatcaa gaccgtcgcc tggtacccgg gatggggcga ggcgcgcatc gccggcatgg
    55501 tcgacgggcg cccggactgg accatctcgc gccagcgcac ctggggcgtg ccgatcgcgc
    55561 tgttcgtgca ccgcgagagc ggcgagccgc atccgcgcag cgtcgagctg atgcgcgcgg
    55621 tcgccgaccg cgtcgagcgc gaaggcgtcg atgcgtggta cgcactggac gccgccgacc
    55681 tgctgggcga agaagccgcg gattacgacc ggatcaccga catcctcgat gtctggttcg
    55741 actccggcgt cacccacgag tgcgtggtca gggagcgcgg gctgggcaag ccggccgacc
    55801 tctacctgga aggctccgac cagcatcgcg gctggttcca gagctcgctg ctgaccggcg
    55861 tggcgatcga caaggccgcg ccgtaccggc agtgcctcac ccacggcttc accgtggacg
    55921 agcacggccg caagatgtcg aagtcgctcg gcaacggcat cgagccgcag gagatcatga
    55981 agacgctggg cgcggacatc ctgcgcctgt ggatcgcctc caccgactac agcaacgaga
    56041 tgtcgctgtc gcaggagatc ctcaagcgca acgccgacgc ctatcgccgc atccgcaaca
    56101 ccgcgcgctt cctgctgggc aacctgcacg gcttcgaccc ggcccgcgac ctggtggcgc
    56161 tggacgacat ggtggggctg gaccgctgga tcgtgcatcg cgcatggcag gtgcaggagg
    56221 aggtcgccgg cgcctacgcg cgctacgact tcgccgcggt ggtgcaggcg ctgatgaatt
    56281 tctgcagcgt cgacctcggc tcgctgtacc tcgatgtcac caaggaccgc ctgtacacga
    56341 tgcggcagga ctcgcgcgga cggcgcagcg cgcagaccgc gatgttccag atcgccgagg
    56401 cgttcgtgcg ctggatcgcg ccggtgctca gcttcaccgc cgacgagatg tggggccaac
    56461 tgcccggcga acgcgccggc gacgtgctgt tcgccacctg gtacgacggg ctggcgccgc
    56521 tggcgggcga cgtgccgctg tcggccaccg acatggacca cctgctggtc ctgcgcgagc
    56581 aggttgccaa ggtgctggag ccgatgcgcg cggccggcgc gatcggcgcc gcgctggatg
    56641 ccgagatcga actgcgctgc gccgtggccg accagaactg gctgtcgccg ctggccgacg
    56701 aactgcgttt cctgctgatc agcggcgacg tgcgggtggt gccggatgcc gacgccagcg
    56761 gcgtgatcgg ggtgttcgcg cagccgaccg ccaagccgaa gtgcgtgcgc tgctggcacc
    56821 gccgcgagga tgtcggcacc gacccgcggc atccggcgct gtgcctgcgt tgcgtatcca
    56881 acatcgaggg accgggcgaa gccaggagct ggttctgatg gcgtccgcgg cgagggcgaa
    56941 cgcgctcccg tggctcgcgg cttcggtggt cgtcatcgtg ctggaccagt ggagcaaggc
    57001 gtgggtgctg gcggcgctgc cggagtacac cgcggtgccg gtcatcgacg gcttctggaa
    57061 ctggtaccgc acctacaaca ccggcgccgc gttcagcttc ctggccgatg ccggcggctg
    57121 gcagaaatgg ttcttcatgg cgctggcgtc cgcgatctgc gcgctgatgg ggtggatgct
    57181 ggcgcgcacc ccgcgccgcg actggaggca ggcgctgccg ctggcgctgg tgatcggcgg
    57241 cgcgatcggc aacgtgatcg accgcgtcgt gcatggccac gtggtcgatt tcatccagtg
    57301 gcatgccggc agcttctact ggccggcgtt caacatcgcc gactgcgcgg tggtcggcgg
    57361 cgccatcggc atcgcgctgt ccggcctgtc cggcggcaag ccgcagcgca aggccggata
    57421 atccgcggat ggacgtcctg ctcgccaatc cccgtggttt ctgcgccggc gtcgatcgcg
    57481 ccatcgagat cgtcaagcgc gccatcgaaa ccctcggcgc gccgatctac gtgcgccacg
    57541 aggtggtcca caaccgcttc gtggtcgacg acctgaaggc gcgcggcgcg atcttcgtcg
    57601 aggaactggc cgaggtgccg gacggcaaca cggtgatctt cagcgcgcac ggcgtttcgc
    57661 aggcggtgcg cagcgaagcc gcgcggcgcg gcctgaaggt gttcgacgcc acctgcccgc
    57721 tggtgaccaa ggtccacctg gaagtcgcaa ggcaatgccg caacggccgc gacatggtcc
    57781 tgatcggcca cgccgggcac ccggaggtgg agggcacgat gggccagtgg aaccgcgagg
    57841 gcgggcgcgg gagcatccac ctggtcgagg acctggacga tgccggcacg ctgcgcctgg
    57901 agcagcccgg caactgctcc tacaccaccc agaccacgct cagcgtggac gacacccgcg
    57961 acatcatcgc ggcgctgcag gcgcgcttcc cggcgatcca gggcccgaag aacgacgaca
    58021 tctgctacgc cacccagaac cgccaggacg cggtgcgcga actggtgggc gagggttgcg
    58081 aggcggtgct ggtggtgggt tcgccgaatt cctccaattc caaccgcctg cgcgaactcg
    58141 ccgaacgcga gggcgccgcg gcctggctga tcgacggcgc cgacgagatc gacccggcgt
    58201 ggatcgccgg tcgccgccgc atcggggtca ccgccggcgc ctccgccccg gacatcctgg
    58261 tgcgtggcgt gctcgaccgc ttgcgcgaac tcggggccgc cagcgtgcgc gagctgcacg
    58321 gcgagccgga ggacatggtg ttcgccctgc ccaaggaact gcgcctgcgg ctggtggatt
    58381 gagccgcccg caaccgctag aatcacgctc cacgccggaa tagctcagtt ggtagagcac
    58441 gtcattcgta atgatggggt cgtaggttcg attcctattt ccggcaccaa tgcagttcga
    58501 cgaagcccgc cttgcgcggg ctttttcgtg cgggtctcga tgggtgcgac ccgcgccggc
    58561 taaagtgcgg catcgcccgc gcatcgatcg tcatggccaa gcccgcctcc aacctgtcct
    58621 cgcagaagcg ccgcaccgtc catgtctgcg gcgagtgcgg cgcggaccac gccaagtggc
    58681 agggccagtg cgatgcctgc ggcgcgtgga atgcgctctc cgaagtggtg ctggaaacag
    58741 ccgcgcaggc ggcagcgccg gcgtcccggc gcagcggttg ggcgggcaag gtggatgcac
    58801 cgaaggtgac cgcgctggcc gacgtgcgca ccgacgcgca ggcgcgcgtg tccactggca
    58861 tcggcgagtt cgaccgcgtg ctcggcggcg gcctggcgga agggtcggtg gtgctggtcg
    58921 gtggcgatcc cgggatcggc aagtcgaccc tgttgctgca ggcgctggcg ctgatggcag
    58981 ggaccttgcc ggggctgtac gtgacaggcg aggaatcgct ggcgcaggtc gcggggcgcg
    59041 cgcagcggct cggcctgccg ctggacggcc tgctggcgtt ggccgagacc tgcgtcgagc
    59101 gcatcctcga gcatgcggtg gcggccaggc caaggctggt cgtggccgat tcgatccaga
    59161 ccctgtggac cgagagcctg gcagccgcac ccggttcggt gggccaggtg cgcgagtccg
    59221 ccgcccggct ggtgcgctac gccaaggaaa ccgggaccgc cgtgttcctg gttggccacg
    59281 tgaccaagga aggcggcatc gccggcccgc gcgtgctgga gcacatggtg gatgccgtgc
    59341 tctatttcga gggcgattcc ggctcgcgct tccgggtcct gcgtgcgttc aagaaccgct
    59401 tcggcgcggt caacgaactg ggcgtgttcg cgatgggcga caagggcctg cgcgaagtac
    59461 ccaacccttc cgcgatcttc ctgtccggtg ccagctcggc gcagcccggc agctgcgtga
    59521 tggtcacccg cgagggcacc cgcccgttgc tggtggaagt gcaggcgctg gtggatgcct
    59581 cgccattgtc gaacccgcgg cgggtcgcgg tcgggctgga gggcaaccgg ctggcgatgc
    59641 tgctggcggt gctgcatcgg cacggtgggg tggtcagcgg cgaccaggac gttttcgtga
    59701 acgtggtcgg cggcatccgc gtgcaggaga ccgccgccga cctgccggtg ctgctggcgg
    59761 tgctgtcctc gctgcgcgac aggccgctgg cggagaagac catcgcgttc ggcgaggttg
    59821 gcctgagtgg cgagatccgg ccggtgccga acggcgagga acgcctgaag gaagcggcga
    59881 cccatggctt caggcgcgcg atcgtgccgc gcgcgaatgc gccgaaggcc ggcagctaca
    59941 agggcatgga agtcatcgcg gtcgagcgcc tggcggacgc gctggaacag gcctgatccc
    60001 gaagcggtcg tggtcgccgc gccacgcggc catgccgccg cctgcctgcg cctggccggc
    60061 cgcggcgggc ggcatgcgtt gtaccatcgt gcgcaaccca gcatccgctg cgtggagccg
    60121 gttcagcgat ggtcgatcag agtgcgcgcg ctgggcgcca gcccgggggg gccgcccccg
    60181 ggttcgcgca cgccggcctg accggctcgt tcctgctgga tcgcgatggc cgggtcctcg
    60241 aagccgatgg cgcgccggtg tctgcgtgct gcctggccga cctggtgccg gagttcgacg
    60301 ccgccgaatt cgcgcgctgg cggatcgccc tcgacggatt tcccgccgcc gtattgcggc
    60361 tcgacttccc gcgcgtggac gcgccggcgg tcagcctgcg gctggaacgc gagatcgacg
    60421 ggcgctacct ggccaccctg ggcggcgtcg gcgacggtga tggcgacgga tatcgcgagc
    60481 ggctggaaaa ccggctggtg ttcgagcaca tgatcgcggc gctgtcgacc gagctgatcc
    60541 atgcgcccag cgaacgcctg gatgggttga tcgagcaggc gctggggcgg atcggaagct
    60601 tcttcggggt cgaccgcgcg tacctgttcc gcttcagcgg cgatcgcgca tcgatggaca
    60661 acacccacga gtgggtggaa gagggcatca gccgcgaggc gcacaagctg cagcaggtgc
    60721 cgatggacca cttcggctgg ctcatgcgcg agttgctggc cgggcgcttc gtgcacatcc
    60781 cggtggtcgc cgacctgccg cccgaggccg ccaccgaggc cgcggagttc gagcgcgagg
    60841 gcatccgctc gctggtgctg gtgccgttcg gcgaggcgca ggcgccggag ggcttcatcg
    60901 gcttcgattc ggtgcgccgt gagcgccact ggtcggcgga catcctgctg ggcctgcggc
    60961 tggtcgggca gatgttctac aacgcgctgc gcgcgcaggt gatgtccaac agcctgatca
    61021 cgctggcgtc gctggatccg ctcaccgggc tgggcaaccg tcgcctgttg ggcgagcagc
    61081 ttgcctacgc gctggagcgc agccagcggg agcagacccg gctggcggtg gtgctggtcg
    61141 acctcgacga tttcaagctg gtcaatgaca gctacggtca ttcgctcggc gacgagatcc
    61201 tcaagacggt cgcgtcccga ctggccgcga gcgtgcccgc cgcggattcc atcgcccgcc
    61261 tgggcggcga cgaattcgtg ctggtggtgc acgtggacgc gctggattcg ctggtgcaac
    61321 aggtcgagcg cctgttcgaa gccatgcgcg agccggtcga attgcgcggc atgcggttcg
    61381 tgctgcgcat gagcgcgggc atcgcgctgt atccggcgga cgccaccgat gccgaggcgc
    61441 tgctgcgcca ggccgacacc gcgatgtacg cggccaaggc cgggggccgc gaccgccacg
    61501 cgttcttcgc accgccgatg gccctggcca gccgcaccac gctgaccctg cgacacgagc
    61561 tgcagcaggc actggagcgt gacgagatcc gtccttgcta ccagccgcgg gtggcgctgt
    61621 cctcgtcgcg ggtgctcggg ttcgaggcgc tggcgcgttg gcaccacccc gagcgcggcg
    61681 tgctgctgcc cggcgaattc ctggagatcg ccgcgcaagg cggcatgctc ggcaggatcg
    61741 accagcgcat cctggcgcac gcgctggccg acctggccgg ttggcgccgc agccatcccg
    61801 attgcatggt gtcggtgaac ctggacgcct ccgacctgca ggacgaggcg ttggtcgccg
    61861 gcctgctgca gcagctggat acggcaggcg acctggccga tgcggtcgag atcgagatca
    61921 ccgagacctc gttgatgcgc aacatcgaac aggccgcgcg cacgctgggg cggctgcgcg
    61981 aggcggctcc cggcctgcgc atcgcgatcg acgacttcgg cagcggctat tcgtcgctgg
    62041 cctacgtcgg caggctgccg gttgccacct tgaagatcga ccgcagcttc gtcgccgagc
    62101 tgggtggcgg caacccccgc aacgcgcgcg cgatcatcca ttcgatcgtc gacctcagcc
    62161 gcagcctggg cttgcacgtg atcgccgaag gcgtggagac gatcggccag gccgacgaat
    62221 tgcgcaggct cgactgcctg gaagcgcagg gattcctgtt cgcccccggg ctgccgcacg
    62281 cgcaggcaag cgcactgctg caagcggggt tcgtgaccgc cgccggggtc cacgcagtgc
    62341 gcagctagcc gcgggcgagc accagctcca gcacgaactt gctgccgaag aaggccagca
    62401 gcagcagcgc catcgccacc agcgtccagc gcaccgcggt ggcgccgcgc cagccgagcc
    62461 gccagcggcc gaccagcagg gcgccgaaag cgagccagga catgacgctc agcacggtct
    62521 tgtgcaccag gtgctgggcc agcaggtcgt cgacgaacag caccccggtc agcaacaccg
    62581 cccccagcag gacgaacccg gccatgatcg tgcggaacag cagggtttcc agttcggtga
    62641 gcggcggcag cgcgcgcagc cagcggtgga actcgcggcg gcgcagggcg cgctcctgca
    62701 gccacaagaa gaccgccagc accgcggcga tcgccagggt cgcgtaggcc agcaacgcca
    62761 gccacgcgtg caacagcagc cgccagtcca gcggctcggg ctgccgcgca gggccatgca
    62821 gcgcatagcc cagcaagccc agcgcggcca gcgggaacac caccacgccc agcgcgcgca
    62881 tgcgcccgct ggcgccgacg accgcggtca ggatcgccat gcccagcccc gccagcgaca
    62941 acgcggcgaa gaaatccagg tcggtgatgc cggtgtcgcg ccacgccatg tagtgggtgg
    63001 cgccatgcag gagcagggcc atgttggccg gcagcagcca accacgcggg accccttcgg
    63061 cgcgctgcac cgacgcgacc agccagccgg tcgcggccag gtacagcgcg atcgcgatga
    63121 gagcgattgt catcgcggca gtgtcgcaca aaggccgacc gcgggatcgc ccctataatt
    63181 gcggcccgtt tccccgattg cgcggcccac gatgttcgaa tccctgaccc aacgcctgtc
    63241 cggcaccatc gagcgcctgc gcgggcgcgg ccgcctgacc gaatccaaca tcaccgaagc
    63301 cgttcgcgaa gtgcgcatcg cgctgctcga ggccgacgtg gcgctgccgg tggtgcaggc
    63361 gctggtgcag cggatcaagg tgcgtgcggt cggccaggag gtgatgcgct cgctgacccc
    63421 gggccaggtg ctggtgcgga tcgttcgcga tgaactcacc gcggtgatgg gttcgcaggc
    63481 ctcggacctc aacctcaacg tgccggcgcc ggcggtgatc ctgatggccg gcctgcaggg
    63541 tgcgggcaag accaccaccg tggccaagct ggccaagcac ctgaaggaaa agcgcaagaa
    63601 gaaggtgatg gtggtcagcg ccgacgtcta ccgtccggcc gcgatcgagc agttgcagac
    63661 cctggccggc caggtgggcg cggtgttctt cccgtccgat gccgcgcaga agcccgaagc
    63721 catcgtcaag gccgcgatcg acgaagcgaa gaagaccttc gccgacgtgc tgatcgtcga
    63781 caccgccggc cgcaccagca tcgacgacgc gatgatggcc gagatcaagg cgctgcacgg
    63841 cgcggtgaac ccggtcgaga cgctgttcgt ggtcgactcg atgaccggcc aggacgcggc
    63901 cgtcaccgcc aagcacttcg gcgaggcgct gccgctgacc ggcgtggtgc tgaccaagac
    63961 cgacggcgat gcccgcggcg gcgcggcgct gtcggtgcgc tacatcaccg gcaagccgat
    64021 caagttcgtc ggcgtgggcg agaagcccga cggcctggac gtgttccacc cggatcgcgc
    64081 cgccggccgc atcctcgaca tgggcgacgt gctgtcgctg gtcgagcagg tcgagcagca
    64141 ggtcgaccac gacaaggcca agaagctggc ggagaaggtc gccaagggca agaagttcga
    64201 cctcaacgac atgcgcgacc agctcgagca gatgcagaac atgggcggcc tgcacggcct
    64261 gatggacaag ctgcccggcg tcggcgcact gccggaggcg gtcaagcagc aggtcaccgg
    64321 caaggaagtg ccgcggatga tcgcggtcat caattcgatg accaagaagg aacgccgcaa
    64381 ccccgacctg ctcaacggct cgcgccgcgc gcgtgttgcg cgtggttccg gcctgacccc
    64441 ggccgacgtc aacaaggtgc tgaagcagta ccagcagatg gaaaagatga tgggcaagct
    64501 gggccgcggc ggcatgaaag gcatgatgcg cggcttgtcc gggatgatgg gcgggcgcgg
    64561 aagcatgccc ccgatgcgct gactttcccg catcgattca aggcgttata cttgccggct
    64621 tacccggcca ttccggccgc tgggcaacac cgagaaagca catggtcaag atccgcctta
    64681 cccgtggcgg cgccaagaag cgccccttct accacatcat cgtcaccgac agccgcagcg
    64741 cccgcgatgg ccgcaacatc gagcgcgtgg gcttctacaa cccggtcgcg caaggccagg
    64801 aaaagcgcat cgagctggac ctggaacgcg tcaagcactg ggtcgacaac ggcgcgcagc
    64861 tgaccgacaa ggtcgccgac ctgtacaagc aggccgccaa ggccgcctga tgccgctgcc
    64921 gcctgttgca ggcggcgctg cccacatgga cgcaccggac ggccgccagc ggaacatcac
    64981 cgtgggccgg ttgcacggtg cgttcggcgt gcgtggcgag gtcaagctgg aaagcttcac
    65041 cgatccgctg cgggcgattg cgagctacca gccctggatc ctgagggacg cgcgcggggt
    65101 cgagcgtgcc tgcgagggcg tgcgcgtccg cgagggcgtg aaaggcctga tcgcgagcat
    65161 gcccgggatc gaggaccggg acgccgccga cgccctgcgc ggggtggagg tgctggtgcc
    65221 gcgctcggcg ctgccgccgc cggcgcccgg cgagtactac tgggtggacc tggagggcct
    65281 gcgcgtggtc aacgcggaag gcgtcgattt cggccttgtc tcccacctgt tttccaccgg
    65341 cgccaacgac gtgctggtgg cgtcgggcga gcgcgaaagg atgatcccgt tcgtgcagcc
    65401 cgatttcgtc cgcggcgtgg atttcgacgc gggcgtggtc acggtcgact gggacccgga
    65461 cttctgagcg caatgccgat gcgcttcgac atcgtcagcc tgttccccgg attcgtcgcc
    65521 cagcttgccg cacatggcgt ggtcgggcgc gccggcgagc gcgggctgct gtcgatcaat
    65581 ggctggaacc cgcgcgattt cgccgagggc aactaccgcc gggtggacga tcgcccgtgc
    65641 ggcggcgggc ccggcatggt gatgatggtc gacccgctgc gggcggcgat ccacgccgcg
    65701 cgcgcggccg acccggcgcc ggcgagggtg gtctacctga gcccgcaggg cacgcgcctg
    65761 acccaggcga aggtgcgcga actggcggcg ctgccgcgcc tggtgttgct gtgcggccgc
    65821 tacgaggggg tcgatgagcg cctgctgcag gcggaggtcg acgaagagct ctcgatcggc
    65881 gactacgtgc tgtccggcgg cgaactggcc gcggcggtgc tggtcgatgc ggtggcgcgc
    65941 ctgcaggacg gggcgctgaa cgacgccgaa tccgcgctgc aggacagttt cgagggcgac
    66001 ggcctgctgg attgcccgca ttacacccgg ccggtggagc acgaattggg ctccgtgccg
    66061 gaggtcctgc tgtccggcaa ccatgccggg atcgcgcgct ggcggcgcca gcaggcgctg
    66121 ggccgtacct ggctgcgccg gcccgacctg ctggacgagg ccgggctgtc caaggccgac
    66181 cgcaccctgc tggacgcgtt ccgggcgcaa tacgccgcgg ccccgcggga cgccggcgag
    66241 ggcggctaag ctcctagcgc acaagggaaa aaccccgcta taatgcgcgg cttcgcttca
    66301 tcccgttcca cccccgtgcc atgacgcggc gtccacgtgc acccgcgcat tacaactaat
    66361 ccaacaggct ccgaccatga acaagccctc gatccacacc ctgctgcagg aattcgaaac
    66421 cgcgcaagtc acgcgcaagc tcccggactt cggccccggc gacaccgtcg tcgtcaacgt
    66481 caaggtcaag gaaggcaacc gcgagcgcgt gcaggcctat gaaggcgtgg tcatcggcaa
    66541 gaagaacgcc ggcctgaact ccgcgttcac cgtgcgcaag atctcgcacg gcttcggcgt
    66601 cgagcgcgtg ttccagaccc acagcgccgc gatcgactcg gtgcaggtca agcgccgcgg
    66661 cgacgtgcgc gccagcaagc tgtactacct gcgcggcctg gaaggcaaga aggcgcgcat
    66721 caaggaagac ctggcgggca acgccaaggc caaggccgcg gcgcttgccg cagcggccgc
    66781 cgccgccgag taaggcgaac gccgcgcgac ctgcaacggc cacctccggg tggccgtttg
    66841 cgtttgagca cgcgcgatgg aggatcccat gccgatgcaa accgaaaccc aacccgccgc
    66901 cggcgtgcgc ctcgacctgt ggctgtgggc ggcgcgtttc taccggacgc gggccctggc
    66961 caagcatgcg atcgagaccg gcaaggtgga tgtcgccggg cagcgcgcga agccttcgcg
    67021 cagcgtgcgc atcggcgatg cgctggtggt tgcgcgtggc gaggagcgct tcgagatcga
    67081 agtgctggcg ctcagcgaca tgcgcggccc ggccagcgtc gcgcaggcgc tgtatcgtga
    67141 atccgcggca tcgcggctgg cccgcgaaca ggcgcgggcg atgcgtgccg ccgcgcacaa
    67201 tggctttcgt ccgcccgaga cccggcccga caagcgcgcg cgccggctga tcctggcact
    67261 gggcgatctc gacgccctct gatcgtccgt gctcgcctgt acccggtgtc tgcccggggg
    67321 ctaggctgaa acggctagtg ccggacatcg cgcttaggcc atttcgggaa tggcgtgtct
    67381 ccccaaggat gcgcaggatt ccccctcagg tttgcatgga gcattggcat gacggtcacg
    67441 gtgttgaagt tggggtcccg cggcgagccc gtccgtaggc tgcaggacgc gttgaaccgg
    67501 aaattgcctg gcgccgtcct gaccgtggat ggcgacttcg gaaaccgcac gctggctgcg
    67561 gtgcggcgct tccaggacga ggcctggctg gtggtggatg gcgaggccgg gcaatgcacc
    67621 cagaacgcgc tgttcgatgc ggaggcctat gcgccggtcc ggcatgtgcg gccgttcatc
    67681 ccgcaaccga ccaacagcac ctgctgggcg gcggcaacgg cgatgctcaa gcgcagcacg
    67741 gtcgccgcgg tcaaggcggc cacgccggcc gacatgtggg acgacaccaa tggcctctac
    67801 aaccagtcgg acgttggcga ctgggtgccg cagacccaac gctacgcgcg tgcgcacgga
    67861 ctgctctact acccgccgtc ttcctggata ccgcgcgcgt tcgccgacct ggttcgccag
    67921 gggcctgtga tggtggacac gctgtgggac acgcgtggct acctgaccag ggtgccctcc
    67981 ggctacctgg gaagcccagg ccacatgacg ctggtggtcg gggtgcgtgg cgacaacgac
    68041 ccgaccgggc ggggtacctc gctgatggtg tacgacccgt gggaaccgaa tgtcggcaag
    68101 cgctggcggg tgagttacca gcgctgggtc ggggaagtca gtacacgcac ttatcggctg
    68161 ttccagtgac gcaggcggag acagccgctc gcgcggccgt tccgtcgtac tgcggggcag
    68221 gaaggcttac cgcaggtcga agcgatccag ttccatgacc ttcgcccagg cagcggcgaa
    68281 atcctccgcg aacttctgct tcccgtccgc gctggcatag acctccgcca gcgcccgcag
    68341 caccgagttc gagccgaacg ccaggtccac gcgggaggcg gtccatttca tcgttccggt
    68401 ggtgcggtcg cgtccttcga aacggttgcc gccggccggt gtccacgccg tgcccatgtc
    68461 cagcaggtgc acgaagaagt cgttgctgag cgtgcccggg cggtcggtga gcacgccatc
    68521 cttggaacca ccggcgttgg cgccgagcac gcgcaggccg ccgaccagca ccgtcatttc
    68581 cggcgcggtc agggtgagca actgggcctt gtcgaccagc aggtgctcgg ccggcaccgc
    68641 ggattcgcgc gccaggtagt tgcggaagcc atcggcgaag ggttccagcg gcgcgaacga
    68701 atcgacgtcg gtctgctcct gggtggcatc ggcccggccg ggcgagaacg gcacagtgat
    68761 gtcgaggccg gcgtccttcg ccgcctgctc gatggctgca ttgccgccca ccacgatcag
    68821 gtccgccagc gagacccgct tgccgccggc agcgctgccg ttgaagctgg cctggatggc
    68881 ttccagcgcg gcaagcaccc gggccagctg cgcgggatcg ttcgccgccc agtccttctg
    68941 cggtgcaagc cggatgcgtg cgccattcgc gccgccgcgc ttgtcgccgc cgcggaaggt
    69001 cgatgccgac gcccatgcgg tggagaccag ttccgacacg gagagtccgc tcgccaggat
    69061 gtcgcgcttg agcgcggcga tgtcgccggc atcgaccagt ggatgctcga ccgcggggat
    69121 cgggtcctgc caggccagca cttccgccgg cacttccggg cccaggtaac gggcgcgcgg
    69181 ccccatgtcg cgatgggtga gcttgaacca tgcccgcgcg aaggcatcgg cgaactggtc
    69241 cgggttctcc aggaagcgcc gcgagatctt ttcgtacgcc gggtcgaagc gcagcgacag
    69301 gtcggtggtg agcatcgtcg gcaccagctt cctcgatgca tcgtaggcgt gcgggatcga
    69361 tggctccgcg cccttggcca cccactggtg cgccccggcc gggctcttcg agagttccca
    69421 ctcgaagccg aacaggtgct cgaagtagtc gttgctccat tgcgcgggcg tcttggtcca
    69481 ggtgacctcc agcccgctgg tgatggtatc ggcgcccgtg cccgcgccga acccgttttg
    69541 ccagccgaag ccttgcgcct cgaggcttcc tgcctccggt tccacgccga cattgctggc
    69601 gggtccggca ccatgggcct tgccgaagct gtggccaccg gcgatcaggg cgacggtttc
    69661 ctcgtcgtcc atggccattc gcgcaaacgt ctcgcggatg tcgcgggctg cggcgagcgg
    69721 gtccggattg ccttcggggc cttccgggtt cacgtagatc aggcccatct gcaccgcggc
    69781 gagcggattt tcgaggcttc gcgcacccgc gtcgttgccc gcatggcgtt cttcgccgcc
    69841 gagccacctg gtttcgcggc cccagtacac atccatgtcc ggttcccagg tgtcctcgcg
    69901 gccacatgcg aagccgaagg tcttgaagcc catggtttcc atcgcgacat tgccggcgag
    69961 cagcatcagg tccgcccacg aaatggccct gccgtatttc tgcttgatcg gccacagcag
    70021 gcggcgcgcc ttgtccaggt tgccgttgtc cggccagctg ttgagcggtg cgaatcgctg
    70081 ctggccgcga ccaccgccgc cgcggccgtc gccgatgcgg taggtgccgg cgctatgcca
    70141 ggccaggcgc accatcaggc caccgtagtg gccgaagtcg gccggccacc agtcctgcga
    70201 atcggtcatc agcgcagcaa ggtcggcctt gagcgcgacg tagtcgagcg tggcgaactc
    70261 cttcgcgtag tcgaagccag cccccatcgg gtcggacttc ggggagtgct ggttcagcag
    70321 gtccaggcgc agctggttcg gccaccagtc gcggttggtg gtcccggcgg tattgccgtg
    70381 gaacgggcac ttcgattcgg tcggcatggt gggctcctgg tgatggcgta cgccagcctc
    70441 caccgtagtc cccgctgcgt tgcagttgaa atcgaatgat cgaacggcat cgatcgtcgt
    70501 ggactatcga attacgacga ttcccgtgat ccggtcgatg ccgggcccgg cgcaccgaag
    70561 ccggtgccga aggctgcgca acgtgcgggg ccaccggcct gcgccggtcc tgatcccggt
    70621 gcgggcggcc gttcgcgcgg gtgccggctc agcccagcga cttgaggcgg tagagcgctt
    70681 ccagcgcctg cttcggggtc aggtcgtcgg gatcgatgcc ggccagtgcc tccagcgccg
    70741 cgctggaggg ggcgaacaag ccgaactgct gcggctggtc cagcgccggc gcgctcagcg
    70801 ggacatgcgg cgtatcgcgg ctctgcgctt ccagctccgc cagccgtgtc ctggcctgtt
    70861 gcagggcggc cttcggcagg ccggccagcg ccgccacctg caggccgaag ctgcggttgg
    70921 cagggccggg gcgcaccgcg tgcatgaaca ccagcgcgtc gccgtgctcg accgcgtcca
    70981 ggtgcaggtt ggcgatgccg ctgccgggtt ccgccagcgc ggtcagctcg aagtagtggg
    71041 tggcgaacag ggtgtagctg cggttcgccg aggccagctg ccgcgccacc gcgtccgcca
    71101 gcgccaggcc atcgtaagtc gaggtgccgc ggccgatctc gtccatcagc accagcgagt
    71161 gttcactggc gtggtggagg atgtaggcgg tctcgctcat ctccaccatg aaggtggact
    71221 ggccgcgggc gaggtcgtcg ccggcaccga tgcgggtcag gatgcggtcg atcggtccga
    71281 tctccgcttt cgtcgccggc acgaagctgc cgatgcaggc gagcagcacg atcagcgcgt
    71341 tctggcgcat gaaagtggac ttgccgccca tgttcgggcc ggtgatgacg tacatgcgcg
    71401 ttgcctcgtc gaagacgagg tcgttcggct cgaacggcgc gtcgcgcagc gcctccacca
    71461 ccgggtggcg gccgcgttcg atgtggatgc cgggggcatc gcgcagcgcc ggccggctcc
    71521 agtccagcgc ctgtgcgcgc tcggcgaagc cggccagcac gtccagttcg gacagcgcca
    71581 gcgcgcagcg gcgcagcggc tccagcaccg cgttcagtgc gtccagcacg ccgtcgtaga
    71641 gcagcttctc gcgcgccagc gcgcgttcgc gcgcggacag caccttgtcc tcgaacccct
    71701 tcagttcctc ggtgatgtag cgctcggcgc cggtcagggt ctggcggcgg gtgtagtgca
    71761 ccggcgcgcg atcgctctgg cccttgctga tctcgatgta gtagccgtgc acgcggttgt
    71821 agcccacctt gagggtggcg atgccggtgg cctcccgttc gcgcgcttcg aggtcgatca
    71881 ggaactggtc ggcattcgtc gacagctggc gcagttcgtc cagttcggca tcgaaaccct
    71941 ccgcgaccac gccgccgtcg cgggccagca gcggcggttg ggtgacgatg gcggcgcgca
    72001 gcatgcgtgc ggtggcgtcg tgctcgccga gttcggcatg cagcgcggcc agccgcggcg
    72061 aatccagcgg cgcaaggatg tcgcgcacgg ccggtagcag cgagagtccg tcacgcaggg
    72121 tggacaggtc gcgcgggcgc gcgctgcgca gggccacccg cgacaggatg cgctccatgt
    72181 cgccgaggct gcgaaacgcc tcgcgcaagt cttcgtcggt gcggtgatcg atcaacgtct
    72241 gcaccgcgtg gtggcgctcg cccacgacgc ggcggtcgcg cagcgggcgg tgcagccagc
    72301 ggcgcagcag gcggccgccc atcgggctga cggttgtgtc cagcacgccc agcagggtgg
    72361 ccttgacgtc gccatcgacg cgcgagtcca gctccaggtg gcggcgcgtc gcggcattca
    72421 tggcgatggc gtcgtcgctg gtttccagtg cgatgctgcg caggtgcggc agccgctgtt
    72481 tctgggtttc ctcaacgtag cccagcaccg cggcggcggc ggcgatcgcc agcgggcgat
    72541 cttcgaggcc gaacgcggtc aggtcgtgca cggcgaagaa ctgcagcagc tggcggcggc
    72601 cggaatcggc gtcgaacagc cacggtgcgc gccggcgcag cccgcgcgag tccagcacgc
    72661 aggcgggcca gccgtcctcg tcgggcacca gcgtttccgc cggctgcagg cgcgccagct
    72721 ccgcttccag cgcgtcttcg ccggccactt cgttggcgcg caggcggccg ctggccaggt
    72781 ccgcccaggc cagcccgtag ccccggccct tgctggacat gccgccgcgg ctgaccgcca
    72841 gcagcagggt gtcgcggcgt tcgtccagca gcgcttcgtc ggtgaccgtg ccgggcgtga
    72901 cgatgcgcac caccttgcgt tccacgatgc ccttggaagc cgccgggtcg ccgatctgct
    72961 cgcagatcgc caccgattcg cccagcgcca ccagccgcgc caggtaacct tcggcggcgt
    73021 ggtgcggcac cccggccatc gggatcggct gcccggccga gttgccgcgc tgggtcaggg
    73081 tgatgtcgag cagccgcgcg gccttgcgcg cgtcgtcgta gaacagttcg tagaagtcgc
//
LOCUS       DAORUK010000002        34553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_800_length_34553_cov_18.275204, whole genome shotgun sequence.
ACCESSION   DAORUK010000002 DAORUK010000000
VERSION     DAORUK010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 34553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34553
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_800_length_34553_cov_18.275204"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /locus_tag="PK818_00370"
     CDS             <1..1597
                     /locus_tag="PK818_00370"
                     /inference="COORDINATES: protein motif:HMM:NF027439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQY81219.1"
                     /translation="GTHYFYHAAKNKLVELIAGERTDAALLAEVEAFYAGLGKTPIRT
                     ADAPGFAVNRFFVPWLNEAVRVYAEGLGSIPTIDAVARAAFGVGMGPFALMNATGVPI
                     AQHAAEGLAAKLGDFYAPAPRLIAQVAARQDWNLDDHASVHGGQEQPEAIRERLLAAA
                     LGCAAALVSEGVCSATDTDLGARSGLRWPRGPFELMAAIGQDAIAGMVGALFARWGMP
                     LPTLPFASSTPIALEWVQAEVHGDTGVIVFNLPDRMNALGEAVMAQLDAAWTRLEADP
                     AVRRVFLCGRGKAFVAGADISFFLDAIDGHDLDRIHAFTVRGQQTLGRIAASAKPSVA
                     LLDGLAFGGGLELALACRYRLGTRNTLLALPETGIGIYPGLGGTQRSARLLGVGVAKS
                     LIATGRRLDAGKALDLGLVDAVIEPPQRYADLAQLPLPTPQSGRQPQPGLEAAFADYR
                     GEIDAATLAQPQLAPFARELARKAPIALATAMRLVDDGRVLPLADALQLELDGLHAIF
                     ATHDARAGLASVLDGSRPSYAGH"
     gene            1609..2871
                     /locus_tag="PK818_00375"
     CDS             1609..2871
                     /locus_tag="PK818_00375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQY81220.1"
                     /translation="MTASATGFRNAHGVGACFDDVYLLAGARTPFGKFCGALSTVSPT
                     DLGIHAARAAIAAAGVDAGAIDQTIVANIGQASADAFFLPRHIGLYAGAPEASTALLV
                     QRICGSGLEVLAQAAEQVALGKAQLVLGVGTDTMSRFPLASYTARQGFALGKPEFTDL
                     LWEALDDTAAVPMGRTADNLAGRYALTRAEVDGFAHQSQVRCAAARAAGFFEGELAMV
                     PGQGAFEAATLKPRRFRCKDKAGLGADEHPRTTTPEALANLPHVFSEAGPTTAGSASG
                     IVDGAAALLVASGDFVRAHGLAPVARLVAHASAGVAPAYMGIGPVPAVRATLAASGMT
                     ADDIGRFEINEAFAAQCLAVARELGIDEARLNVNGGAIAIGHPLGATGVRLALTLART
                     LARDDAGFGIASACIGGGQGTALLLQRC"
     gene            2865..3617
                     /locus_tag="PK818_00380"
     CDS             2865..3617
                     /locus_tag="PK818_00380"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HQY81221.1"
                     /translation="MLSRYRQRVALVTGAGRGIGAAIARRLHDEGASVVLADRDGDAA
                     AALAAELGGGRVLAVAVDVTARAGLEALMERIKSDLGGLDLLVNNAGIIRDGWLSRLS
                     EADWDSVLAVNLKGPFLCCQAALPLLRERAGSAIVNIASRSWLGNPGQANYAASKGGV
                     VSLTRTLALELARDGIRVNAVAPGLIDTPMTQAMPGEARERLLRMQPSGQMGAPADIA
                     AAVAFLGSADAAFISGQVLHVDGGKSCGLLGL"
     gene            3620..4774
                     /locus_tag="PK818_00385"
     CDS             3620..4774
                     /locus_tag="PK818_00385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQY81222.1"
                     /translation="MTALPPPASTPTARIVAARRTPIGRSHADSGALRDMRADELLAR
                     LIAEVTRTLPDAGALDDVLVGCVGQHLEQGKNIARLASLLAGLPASVPGVTFNRLCAS
                     SLEAFNTASMRIAGGQDQLLLAGGIEHMQHVPMAAAVDYHPDLLARPPFPFNNMGLAA
                     EWLAVRDGIGRATQDAWALESHRRACHARAHGRFRREMLAIDGIADDQCPRADASRDA
                     LAALRPAFREDGSVTAGNASPLSDGASLVLLASAQACVRDGLRARAEVLGSVVVGLAP
                     EAMGDGPVLAIRKLLARFGLATGDIDRYEINEAFAAQVVSNVRALALVAERVNPDGGA
                     IALGHPLGATGTRLVTTLLHGLEADDGELGIAALCVGHGQGVATLIRRLH"
     gene            4774..5277
                     /locus_tag="PK818_00390"
     CDS             4774..5277
                     /locus_tag="PK818_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQY81223.1"
                     /translation="MDAAAPTSPTATETLALQVNGQRHVRAVPPGTSVLDFLREQLGL
                     TGSKRGCDLGECGCCAVLVDGRPMLSCLLPAADMVEAEITTIEGIGCGNALHPLQQAM
                     IDHGAIQCGYCTPAMVLNGVALFAADPHPDERALQTAIAATTCRCTGYNAIRNAFRAV
                     AAGGEAG"
     gene            5274..7661
                     /locus_tag="PK818_00395"
     CDS             5274..7661
                     /locus_tag="PK818_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQY81224.1"
                     /translation="MKPYRIVGQPQAQLDARDKVTGRARYTDDLRVPNPLHAAILRSP
                     HASARILRIDTRRALALPGVRAVLTGADFERTFGVLPISKDEPALARERVRYVGEPVA
                     AVAAETAEIAAQALALVAVEYAPTPPVLDARHALKPTDDPLHPELKRADNLHKQVEQR
                     FGDPEAAFAAAPIVIEKRFRYAAVTHAFTEPHAVQVEIDGRGNLTVYSATQVPHYVHL
                     ALAEVLGIDEHRVRVVKPHLGGGFGGKSDPFPHEMIAAKLALVSGRNVRLRFSREDVF
                     FSHHGRHPTQLTMKLGFDPARGITALTGDILIDGGAYGSFGVVTTYYNGVLLQGPYPV
                     PNFGFRCRRLYTNTPMCGAMRGHGGVNPRFAAEVLMDMALHQAGLDPCQGRIDLLHHA
                     NTLTVNEFRITSAGLKRGLEEAMHRSGWREKHGKLPFGKGIGVACGFFISGSALPIHW
                     NERPGSTVRMTLEFDGAITVYSGASDIGQGSDTVLAMMAAEVLGVDLDQVKVVAADTR
                     LTPVDLGSYSSRVTFMAGNAARNAAIRMRRLLVAGACRKLGLDEPVYPAPGPPADFFR
                     QGQRAPDFEADDLGANPDYRDGFAFGGGRIRHLASGADVAWLDAVHAAIEGPGILQTV
                     GNYRSPKLGGTFKGAGAGLSPAYSFTAFIVEVTVDPETGHVRVDKATCAHDCGFALNP
                     LAVRGQIEGCFHMGMGQALMEEVLHEGGAVQNAGFLEYKIPSPFEQPEIDIVLCDSDE
                     NEGPFGAKEAGEGPLAPVIPAIANAIFDAVGVRLAEVPLRPDRVLAALRKQAGVA"
     gene            7658..8629
                     /locus_tag="PK818_00400"
     CDS             7658..8629
                     /locus_tag="PK818_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HQY81225.1"
                     /translation="MTPAFDLQRPRSLDALHALLDGLARRQQRFAISAGGTDLVPQLK
                     LGLATPEVLVSLGAIPALRGITREPDGRVRIGAMTPLATIIADPLLHAVAPVLGEAAA
                     RVASAPIRERATLGGNVLADHRCNFFNQSPLNRAAHGPCFKAGGDVCHIVKSAVRGQL
                     PQCQARIVSDTTPALLVLDAELQMHSPRGERRIALADFHLGDGIERHRLAADEVLLAI
                     TLPSRPGRVAAYEKLAARANLDFPSLGIALARDGDRLHAAMTGIGVRPASARAALADG
                     ALATLGESLASANPVYDQDGFPRDYRKRMIAAYVRKAARRIGGDAWT"
     gene            8620..9804
                     /locus_tag="PK818_00405"
     CDS             8620..9804
                     /locus_tag="PK818_00405"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HQY81226.1"
                     /translation="MDVKQVMREYFLDLDAAAKDPARKVAWCTSVGPAEILRAMGFAV
                     YFPENHGAMLGATRVAGDTIPVANANGYSPEICSYLTADVGACMKGITPLTRAYGIEA
                     VPRPDVLVYCTNQCKDVMHWFAWYARQWNVPLLGVHTPNHLPEVEPEHIADVAAQYRR
                     LITQLEAVTGERLDPQALRQAVARSKQATDLWAEVLATAMARPAPLSFFDGAVYMGPI
                     VVLRGTQVATDYYRALLDEMRARVAASRAAVPGERLRLYWEGMPVWGRLRQLSDLLAR
                     NRTCVVASTYCNSWIFSDFDPDAPLESMARAYLSIFINRDDAYKERYIARLAEEFGID
                     GVVYHAARTCPNNTNSHYNLPRRLQAAGVPGIVMDGDLNDLRCFSDEQSTTLLEAFME
                     TL"
     gene            9801..10712
                     /locus_tag="PK818_00410"
     CDS             9801..10712
                     /locus_tag="PK818_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HQY81227.1"
                     /translation="MSTALASHAEAAAAPADASPRVYWGVDCGSAEIKVAAVDADGQV
                     LLTRKQRTLFPLAEHVHRALAGAPGAASPLHEDDRAALPEGGVPRLRAGQRVYATGYG
                     RNHIPFVHGACTEIKAHYLGVQHQLGLAEDYTIIDIGGQDAKVIGATPSRVGQFAINR
                     KCAAGTGAYIEELAHRLEVPMQSLPALAAAHDQELTLNSYCTVFSGQEVIKLLMHGER
                     VENLVHALYRSVVKRVLEMTAIDTPTLVFSGGVMAHHQNLMPLFGERFERGVHTILAP
                     NAQFCGAIGAAIHGYNEEKKDGFPHHQ"
     gene            10690..10980
                     /locus_tag="PK818_00415"
     CDS             10690..10980
                     /locus_tag="PK818_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HQY81228.1"
                     /translation="MAFRITSECNLCDACVPECPNEAITAGSRIYVIDPMKCTECVGF
                     FDEAQCAAVCPQECCEPDPDRPEDNQALLQRARALHPDEEFTDDAPSRHATN"
     gene            11003..12880
                     /locus_tag="PK818_00420"
     CDS             11003..12880
                     /locus_tag="PK818_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HQY81229.1"
                     /translation="MNQATASPAAIEPDDACLLAEITLKFAGDSGDGIQLAGQQFAQL
                     ASAAGERVRTLPDFPAEIRSPVGTLGGVSGFQVRAGGEQVLTPGDRLDLLVAMNPAAL
                     RSNLDKLKPGAIVLVNEDAFGAANLGKAGFAANPLEDGSLRAYRVFSVPMGMLTRNAL
                     KGLPLSTREKDRSRNFFALGLICWLFPRPLEDVEAFIERRFAANPELAEANRRAFRTG
                     WNYGETAEFFVSPLRVAGSERLREPGVYRFVTGNAAVAKGMVAAARRAGLPLLLGGYP
                     ITPATEVMQELARLREPDVRMFQAEDEIAAIGSAIGASFAGALGATSTSGPGLSLMGE
                     FLNLAVIAELPLVVVDVMRAGPSTGIPTKTEQADLLQALHGRHGESPMPVLACASPAD
                     CYHATVEACRIALRYMTPVIVLSDLSLSGSAEVWREPHVDELAPFHVHRVTDPDGFQP
                     YRRDPETLARPWALPGTPGLEHVIGGLEKAGDSGAVCYEPENHEAMVQLRADKIDRIA
                     QDYPATEIHGDPDARLLLLGWGSTHGAIREATDQLRAAGRPVACLHLRHLNPLPRDLA
                     EVLRRHPQVAVLENNSGQLWRHLRASCLLDLKRLGKVQGTAFHVPEIIAYAESLLETP
                     A"
     gene            12877..13821
                     /locus_tag="PK818_00425"
     CDS             12877..13821
                     /locus_tag="PK818_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HQY81230.1"
                     /translation="MSETATALAEQADDRRQDFLSATTPRWCPGCGCFAVLKSLTATY
                     AALGIPREKLLTISGIGCTSRLPYYTRTFGFHTVHGRAPTVAMGAKLANPELSVWLIS
                     GDGDAMAIGGNHTMHLLRRNPDIKMLMLNNQIYGLTKGQASPTSPAGLETKSTPFGVQ
                     DNPINPVNLALACGATFVARVPDTNNAMMEEVFAAAHAHRGVAFVEVLLNCVTFHDGA
                     YEQITSKATRDDHSVRLRAGQPLRYGKDGNRVLVHAHGRIEAQVLEDGQPLPANAVVH
                     DPTDPGLAQQLALMALPELPCALGIFRQVQAPVYGQAC"
     gene            13838..14269
                     /locus_tag="PK818_00430"
     CDS             13838..14269
                     /locus_tag="PK818_00430"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQY81231.1"
                     /translation="MDSLYVHRAPVRFTHTDPAGYVFFPRFFEMFQAAVEDWFTHCLG
                     LNYAEQILRHGIGFPTARTECDFMRPCRLGEVLELRLVLEQVGRSSMTLRFDGSVHGE
                     ARLRARSVLVAIEMQHGRPQPIASVLRERLQAYLALCEAHA"
     gene            14262..14849
                     /locus_tag="PK818_00435"
     CDS             14262..14849
                     /locus_tag="PK818_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQY81232.1"
                     /translation="MPELAALVLAAGAGRRFGAIKPLAEVRGQPLVRHAIALADAVQP
                     CSLQVVLGAQHEAIAARLPDSAGVIVHEGWQRGLGSSIAAGIRGLAGQPDGVLLLLVD
                     QVALRTQALQALASRWRATPEAVACADYDGLPGVPAILPRRLFAALEALDGERGAKPL
                     LLAEGDAALRLPMPEAAIDIDTPTDLARWLATSPD"
     gene            complement(14860..16161)
                     /locus_tag="PK818_00440"
     CDS             complement(14860..16161)
                     /locus_tag="PK818_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81233.1"
                     /translation="MSALLSLEQAPPFSVPLRFFLTAPLFGVLAAALLGWAGPQALAS
                     RWTDAALALSHLLALGVLSMVMCGALLQMLPVLVGVRVASPRLLAGACHGLLVTGTLA
                     LAAGFLGAGALAFRLAAVLLGLGLGGFLLALGWGLARAPNIQDSVRGMRWAALGLLLV
                     LALGLRLALGHAGLGLDLWRWPLTDLHLAVGLVGWIATLVAVVAWQVVPMFQMTPAYP
                     AALRRVLPPATFALLAWLALAKWLGWPLPWLPWLLLAVLLGGFGLTTLRLLGQRRRKL
                     GDSSLAYWRLGMAMLLLAALAAMLAWWLPGAVAERLLVTATVLFLAGFALSVASGMLY
                     KIVPFLVWLHWQQRLSSRLDLRHQLVLPNMRSILPEARCRRQLQCHALALALLLAAVW
                     LPVLVRPAALLWAASFALLGMNLLQAGLLYQRECRRIEAGG"
     gene            complement(16158..16424)
                     /locus_tag="PK818_00445"
     CDS             complement(16158..16424)
                     /locus_tag="PK818_00445"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HQY81234.1"
                     /translation="MAASGGERAIDVRGLPPCEPMERILAACDALQPGQRLRALLSRE
                     PLPLFPILARRGLAWRVLEVTDDRCELLVWPAGEADGAVADRDG"
     gene            complement(16424..16852)
                     /locus_tag="PK818_00450"
     CDS             complement(16424..16852)
                     /locus_tag="PK818_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HQY81235.1"
                     /translation="MLTDFLTNEHRDCDALFADAENAAHEGDLAAAGSAFAAFAESLR
                     QHLAIEEEVLFPAFEARTGMAGGPTAVMRMEHVQMRTLLEQLQRALDAGSLDDFLGLG
                     ETLNVLVQQHNMKEEQMLYPMCDRALGDQADELLQRLQGH"
     gene            complement(16846..17910)
                     /locus_tag="PK818_00455"
     CDS             complement(16846..17910)
                     /locus_tag="PK818_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HQY81236.1"
                     /translation="MHAANVYRNVPGAPAKAALDACLADFARPRPGVLALVLEVAGHA
                     YAKAGSVVHIGAGGERCGWISPGCLEGALLEAAAASGQDLRPRLLELDNLDLADVFAG
                     SGAGCRGHQRILLLPLAELPALQPILARYRHAGDPLALAVTLPGTLSATCGDLHGEWP
                     LALADAAAGAPAQAWRLRLAPLPRLLACGAGPESELLLPLLAQLGWRVELVESRPAWL
                     HCGQWVERQLAALPGAGEDRGHDGVLLMAHQFDRDRAALEALAEWPRLPAYIGLLGPE
                     TRRDDLLATLPPAVRARLHGRLESPAGMALGGRGPAAIALSLAARLQALACALDGEQG
                     HAAAAVAESRPHPSHEPPAC"
     gene            complement(17923..19293)
                     /locus_tag="PK818_00460"
     CDS             complement(17923..19293)
                     /locus_tag="PK818_00460"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HQY81237.1"
                     /translation="MPTIIEPFRIHSVEPLRITTAEERHAAIAAAGYNLFAVHSDDVL
                     IDLLTDSGTGAMSSEQWAGIQRGNEAYAGSPSWYRFEAAVKALFPFRHVIPTHQGRAA
                     EKILMAVIGGPGRIIPNNTHFDTTRANIEATGAEAVDLVIPEGLDPALEHPFKGNMDL
                     ARLDALLRERRADIPAVFVTITNNSGGGQPVSLENLRGVRALCDRHQVPLFLDACRFA
                     ENAWFIREREPGQGGREVAEIVREIASLADGMTMSAKKDGLANIGGWLAMHDDALAAS
                     CRNLLILTEGFPTYGGLAGRDLDAIAQGLVEVVQHDYLRYRIRSTGYLGDGLHAAGIP
                     TMRPVGGHAVYIDARRMLPHIPPLQYPGQALAVALYEAGGIRSCEIGTVMFGRRPDGS
                     EQPAALDLVRLAIPRRTYTQSHIDYVIEVCAEVAARSAGLGGYRITEEPAALRHFTAR
                     FEPLQG"
     gene            complement(19375..20037)
                     /locus_tag="PK818_00465"
     CDS             complement(19375..20037)
                     /locus_tag="PK818_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HQY81238.1"
                     /translation="MQASGSAPSPHPRAHHAREELANALTHGVGAAAALAAGAVLVTL
                     AAVYGDGWQLAGAIVFGVALLLLYTASTLYHAISHPVVKGRLKVFDHCAIYLLIAGTY
                     TPFTLVGLRGSIGWWLFGTIWTLALAGVVFKLFYTGRFQRLSTLIYLAMGWLIVVAAK
                     PLAHALDGATLAWLAAGGIAYTLGTVFYMRPGLRWSHAIWHGFVIAGSVCHYVAVLRH
                     LL"
     gene            complement(20054..21346)
                     /locus_tag="PK818_00470"
     CDS             complement(20054..21346)
                     /locus_tag="PK818_00470"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HQY81239.1"
                     /translation="MGQSVVVLGAQWGDEGKGKIVDLLTQDIGAVVRFQGGHNAGHTL
                     VIAGKKTVLHLIPSGILRDDALCLIGNGVVLHPGALQKEIAELEGNGVEVRSRLKISP
                     ATPLIMPYHIALDQAREKAAGKSAIGTTGRGIGPAYEDKVARRGIRVADLHYPNELAE
                     KLRVALDYHNFVLTQYLKVDAVDYQQTLDEALAFGEYVEPMKADVAGILHDLRKAGKK
                     VLFEGAQGSLLDIDHGTYPYVTSSNTTVGGALAGTGVGADAIDYVLGIAKAYATRVGG
                     GPFPTELDDAIGQGIRDRGQEYGATTGRPRRCGWMDIVALKRAVAINGISGLCITKLD
                     ILDGMEKLNICIAYEYRGKRTEYAPLDADGWDECTPVYLEFPGWSESTAGITDWDKLP
                     PAARAYLRALEELAGCPLAIVSTGPDRDANIVLRDPFA"
     gene            complement(21429..21617)
                     /locus_tag="PK818_00475"
     CDS             complement(21429..21617)
                     /locus_tag="PK818_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005414170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HQY81240.1"
                     /translation="MSDLWAALCLVAVLEGLFLFAAPGAWKRAAEQLQQLPDEHVRRV
                     GGIVLILGIVSLWLVRGG"
     gene            complement(21620..22483)
                     /locus_tag="PK818_00480"
     CDS             complement(21620..22483)
                     /locus_tag="PK818_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006383787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HQY81241.1"
                     /translation="MKFPAWVPAILAGLLLLLGSVFVVSEGQAAIVLNLGKVVRTDIG
                     PGLHFKLPLIESARVFDRRLQVLSAEPERYLTSEKKDVSVDFFAIGKIENVRDFYRAT
                     GGDESIAAQRLAPIIKDALRNEINARTLQEVVSGDRAKVLAAQLVSINKGAAVLGVKI
                     FDIRIKRIDLPTGGEVIGQVYRRMSAQRKQVASQLRAEGEELSQKIQSEADRQRAVIA
                     AEAERDAQKLRGEGDAEAARLYAEAASRDPGFYAFQRSLEAYRAAFAGGDSVIVLERD
                     DPFLQYMRSDR"
     gene            complement(22480..23568)
                     /gene="hflK"
                     /locus_tag="PK818_00485"
     CDS             complement(22480..23568)
                     /gene="hflK"
                     /locus_tag="PK818_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HQY81242.1"
                     /translation="MAWNTPGNSSGNNNRGKRGGGIGGLLDRLPGFGGGFGDGGALRW
                     LWVVLGLWLAFNCFVLVTEQQRGVVLRFGEFHRTMTPGPHLKLPWPLERVIKVNATQI
                     STYSETVPVLTRDENIVSVEINVQYRIGDAQLYLFGTRDANEVLKEATLSAVREQVGR
                     SDLDTVLSARSALSVSSKQRLQAAMDTYRTGLVVTELNLPNARPPEEVKPAFDDVNSA
                     QQDKDRAKNEAEAYASKVVPEARGQAARVRTEAEGYKTAAIARATGDAERFTLLVEQY
                     KAAPEVTRKRLWLETVQRVLADNRKVVGGDGRQLIYVPMAGATQVQPALPAQAILPDL
                     VSPTVEATADPARPGRQGRSAGREENVR"
     gene            complement(23641..24948)
                     /gene="hflX"
                     /locus_tag="PK818_00490"
     CDS             complement(23641..24948)
                     /gene="hflX"
                     /locus_tag="PK818_00490"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HQY81243.1"
                     /translation="MFERSRRGEHALLIQPHAGGPPGEGVLEEFAELARSAGASVAAV
                     LTAKIDRPNPATLIGSGKLEEVKAACEATGADLVLVNHLLSPVQERNLERALERRVVD
                     RTGLILDIFSQRARSAEGKLQVELAQLKHMATRLVRGWTHLERQRGGSIGLRGPGETQ
                     LETDRRLLQKRVDMLSKRLDKVEVQHTQMRRARVRSELPRVALVGYTNAGKSTLFNAL
                     TGASAYAADKLFATLDPTVRRIELSGGGVVLADTVGFVRDLPHELVAAFRSTLSEARE
                     ADLLLHVVDADDPLRDERMAQVDEVLREIGAGGIPQLLVFNKIDRIEGAAPRIDQRGE
                     DGHAVWVSARDGLGIDLLREALGGKLGLQRVVGEIVLPTDAGRLRARLHALDAVLGEE
                     HDGDGWRLRVDLPVADAARLAAQADGAPLRPLLPEAEPEALES"
     gene            complement(25028..25285)
                     /gene="hfq"
                     /locus_tag="PK818_00495"
     CDS             complement(25028..25285)
                     /gene="hfq"
                     /locus_tag="PK818_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HQY81244.1"
                     /translation="MSKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGTIESFDQFVV
                     LLRNTVSQMVYKHAISTVVPARNVRVGPTGADGDDEGGDEA"
     gene            complement(25422..26375)
                     /gene="miaA"
                     /locus_tag="PK818_00500"
     CDS             complement(25422..26375)
                     /gene="miaA"
                     /locus_tag="PK818_00500"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HQY81245.1"
                     /translation="MTLDNRPRAIALMGPTASGKSAVALELAQRLGGEIVSVDSALVY
                     RGLDIGAAKPTRHELAQVPHHLVDLRDPWQPYSAAEFAVDARGAIDGIIARGRLPILA
                     GGTGLYFRALLEGLADMPEADEATRAAITAEAAERGWAALHAQLAQVDPVAAARIHAT
                     DAQRIQRALEVYRLSGRPISGWQRQAQRARLPLKVLKLVLAPPDRNLLHERIAERFDA
                     MLAAGFLDEVRALRASPELAAHPHPLDLPALRAVGYRQAWEFLGGESTPAEFRERAIA
                     ATRQLAKRQFTWLRGELDARRFDPMTQRTRLDAAVAGFLGG"
     gene            complement(26398..27891)
                     /gene="putP"
                     /locus_tag="PK818_00505"
     CDS             complement(26398..27891)
                     /gene="putP"
                     /locus_tag="PK818_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HQY81246.1"
                     /translation="MTASTPLLITFGIYLLAMLGIGFVAWKRTRDFDDYILGGRSLGG
                     YVTALSAGASDMSGWLLMGLPGALYLSGVSEAWIAIGLVVGAWANWRFVAGPLRVYTE
                     RTDNALTLPDYFTTRFAADGKLLRIVAALVILVFFAVYCASGIVAGARLFESVFGLSY
                     AQALWWGAAATIAYTLVGGFLAVSWTDTVQATLMIFALTLTPLVVIIGSGGFGESLGA
                     IQQLDPAKLDWFKGGELGLVGIVSLLAWGLGYCGQPHILARFMAAESLAVIPQARRIG
                     MTWMVLCLLGAMATGFFGIAYFAQHPQQAGAVADNHERVFIALSAALFNPWIAGVLLS
                     AILAAIMSTLSCQLLVCSSALTEDFYRGFVRPRAGERELVWVGRAAVLAVALLAIFIA
                     RDPDSRVLGLVSYAWAGFGAAFGPVVVFSLSWQRMTRQGALAGIIVGALTVILWKQTG
                     SALYEMVPGVIAASLAIIAGSLLSRAPSAEVQARHQQVQLSLREQGY"
     gene            27974..29056
                     /locus_tag="PK818_00510"
     CDS             27974..29056
                     /locus_tag="PK818_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HQY81247.1"
                     /translation="MPFWKRLGFAFVFVVPALMPAAAWLGTRGGRPDLMAWFPLFFLF
                     VLLPLADYAVGHDPRNPAAADVAALEQDRWFRLLTLLAVPVQYATLAWSLQHWAQAPF
                     SLAGSIGWLASLGVVGGVLAINVAHELIHKDTRLERAAGGLLLASVGYHGFKVEHVRG
                     HHVHVSTPEDASSARFGQSLWHFLPRALLRNTANGWRLEAARLARLGKPWWHWRNELL
                     GWTLLWLALAAAAWAWLGNAGLVAFLVQGVVAACALEIINYIEHYGLERVRLANGRYE
                     RTTHLHSWNSDYALTNLLLFHLQRHSDHHETPRRRYQSLLHHADSPQLPGGYASMFVL
                     ALFPPLWFRAVHPRLHAWRARIASGG"
     gene            29142..30692
                     /locus_tag="PK818_00515"
     CDS             29142..30692
                     /locus_tag="PK818_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HQY81248.1"
                     /translation="MPALLRSLLLGALLLLAGCAQLPARPQLPYQAALPVAEGTRLDA
                     EVAPAVARHPGASGFRLVADGMEAFALRGHSARSAGRSLDIQTYIWHDDLTGRLLAAE
                     VLDAADRGVQVRVLLDDLDARAKSQALLALDSHPRISIRIFNPLATRDGMLGTALEFA
                     SDFERVNRRAHNKSWIVDGRLAVAGGRNLGDEYFMASPEANFQDLDLLVAGPAVAALA
                     DAFDSYWNSESAWPIRALEPRKPQGEALQAVRESLRQSTSDAGKEAWVQALGEPDALA
                     RIRVGHLGMRWTQQWQLLQDAPDKLVQQVQPIAGTDASPILAGLRSAMQSSQREIAII
                     SPYFVPGEEGANSLVAGVRGGLRIRILTNSLASNDVAAVHGGYVRHRGVLARGGVELW
                     EMKPRPGRAQGSLLGSSGARLHGKAAIFDRRTLFVGSLNLDPRSVQLNSEQGLLVTDE
                     VLAAQLSAMFDNMTRPENAWSIGWDPARGRVSWSDGNGTLDREPEAGLSRRVVARLAY
                     WLPVDPLL"
     gene            complement(30693..31586)
                     /gene="folP"
                     /locus_tag="PK818_00520"
     CDS             complement(30693..31586)
                     /gene="folP"
                     /locus_tag="PK818_00520"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006453304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HQY81249.1"
                     /translation="MFDTIPQLECNGRILRLDRPQVMGIVNATPDSFSDGGLHFDAAA
                     AIAQGLKLAAEGADILDIGGESTRPGAQEVPLDEELRRVVPVIERLAKETSLPISIDT
                     SKPEVMRAAVAAGAGMINDVHALRREGALDAAAQLGVPVVLMHMQGEPGGMQAAPHYD
                     DVVGEVHRFLAERIFAAEMAGIARKRIVVDPGFGFGKTLQHNLQLLAQLQRFTELGVP
                     VLAGLSRKKSIGELTGREDPRERIAGSVAAHLIAAQRGAMLLRVHDVAATADALKVWN
                     AVAAVPPPRIEAKPAIAWPED"
     gene            complement(31592..33421)
                     /locus_tag="PK818_00525"
     CDS             complement(31592..33421)
                     /locus_tag="PK818_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY81250.1"
                     /translation="MATTGGFIAELKRRNVFRMAGLYLVTAWLVVQVGATLLPVFDAP
                     PWTMKALVMTLAVAFVPALAVAWMFELTPQGFRRDAEVPAAESIAPRTARTLDRAIIV
                     LLALALGYFGFDKFVLSPRREEALVAEATRAAAVRAAALPARRGNSIAVLPLANASGD
                     ASQLFFSDGLSENLIDALSKFEGLRVIGRTSSFRFRDSKEDARSIGAKLGVAYLLAGS
                     VQRAENTVRIRAEVVRTDDGSAIWTNLYDKPYGDLFALQDELTRAIAVVLKAKLLAPE
                     AKRQDERPPNGSVEAYSAYLRGKFYADRGEDGDMRLAIAEMTRATRLDPKYASAWAAL
                     SRNWTTLAALGLSGDEAQNAYAEARKAGDVALTLAPDLGDVYVARGWLLENQLDWRGA
                     TEAYQRGLELSPGNLQTMFSKASMLALQGQLDEAITLTRQALDNDPMSPNWWNWYSAY
                     LSAVGRLDEAEKAIRDSIELRPQGSSAWAQLTIIELQRGDAQAALDAARREPEGVWRD
                     IAVAMALQAGTDRAAADAALQELISDYGDVAAYQVAQVQALRNDADAAFEWLEKAHAT
                     RDPGIGNLLIDPLVMRYKTDPRLAAFCAKVGLPAPANSQTKGI"
     gene            complement(33644..>34553)
                     /locus_tag="PK818_00530"
     CDS             complement(33644..>34553)
                     /locus_tag="PK818_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HQY81251.1"
                     /translation="KGREQILKVHMRKVPLHDDVRADLLARGTPGFSGADLANLVNEA
                     ALFAARRNKGQVEMQDFEDAKDKIFMGPERRSMVMREDERRNTAFHESGHAVVAMSLP
                     KADPVHKVTIMPRGWALGLTWQLPEHDQLSNYRDKMLEEISILFGGRIAEEIFVGQMS
                     TGAANDFERATKLARAMVTKYGMSDALGTMVYADDKDSGFGLQNKTISEATQQKVDAE
                     IRRILDEQYGVARRILEDRREAVEAMSAALLEWETIDAEQVRAIMEGRKPSPPAGWVE
                     KTARPGKDEDGGPSTAQVVGGPAEQT"
BASE COUNT         5074 a        12667 c        11865 g         4947 t
ORIGIN      
        1 cggcacgcac tacttctatc acgccgccaa gaacaagctg gtcgagctga tcgcgggcga
       61 acgcaccgac gccgcgctgc tggccgaagt cgaagccttc tacgccggac tcggcaagac
      121 gccgatccgt accgcggatg cgcccggctt cgcggtcaac cgcttcttcg tgccctggct
      181 caacgaggcg gtgcgcgtct atgccgaggg tctcggcagc atcccgacca tcgacgccgt
      241 ggcgcgtgca gccttcggcg tcggcatggg cccgttcgcg ctgatgaacg ccaccggcgt
      301 gccgatcgcg cagcacgcgg ccgagggact ggcggccaag ctcggcgact tttacgcgcc
      361 ggcgccacgg ctgatcgcgc aggtcgcggc acgccaggac tggaacctcg acgaccacgc
      421 gagcgtgcac ggtggacagg agcaaccgga ggcgatccgc gaacgcctgc tcgctgccgc
      481 actcggttgc gccgcggcgc tggtcagcga gggcgtgtgc agcgccaccg acaccgacct
      541 cggtgcgcgt tccggcctgc gctggccgcg cggtccgttc gagttgatgg cggcgatcgg
      601 ccaggacgcc atcgcgggca tggtcggcgc gctgttcgcc cgctggggca tgccgcttcc
      661 gacgctgcca ttcgcaagca gcacgccgat tgcactcgag tgggtgcagg cggaggtcca
      721 cggcgacacc ggcgtgatcg tgttcaacct gcccgaccgc atgaacgcgc tcggcgaagc
      781 ggtgatggcg caactggatg ccgcatggac gcggctcgag gccgacccgg cggtgcgccg
      841 cgtgttcctg tgcggccgcg gcaaggcctt cgtcgccggc gccgacatca gcttcttcct
      901 cgacgccatc gacggccacg acctcgatcg catccacgcc ttcaccgtgc gcgggcagca
      961 gacgctcggc cggatcgccg cgtcggccaa gccgagcgtg gcgctgctcg atggcctggc
     1021 cttcggtggc ggactcgaac tggcgctggc ctgccggtat cggctgggca cgcgcaacac
     1081 cctgctggcc ctgcccgaga ccggcatcgg catctacccc gggcttggcg gcacccagcg
     1141 cagcgcgcgc ctgctcggcg tcggcgtggc caagtcgctg atcgccaccg gccgccggct
     1201 cgatgccggc aaggcactgg acctcgggct ggtcgacgcc gtcatcgaac caccgcagcg
     1261 ctacgccgat ctcgcgcagt tgccgttgcc gacgccgcag tccggtcgcc agccgcagcc
     1321 gggactggaa gcggcgttcg ccgactatcg cggcgagatc gatgcggcga cgctggcgca
     1381 gccgcagctc gcgccgttcg cgcgcgagct agcgcgcaag gcgccgatcg cgctcgccac
     1441 cgcgatgcgg ctggtcgacg acggccgcgt gctgccgctg gcggacgcgc tgcagctcga
     1501 actcgacggc ctgcacgcga tcttcgccac ccacgacgcc cgcgccgggc tcgccagcgt
     1561 gctcgacggc tcgcgcccga gctacgccgg acactgacag ggccccgcat gaccgcttcc
     1621 gccaccggct tccgcaacgc ccacggcgtg ggtgcctgct tcgacgacgt gtacctgctt
     1681 gccggtgcac gcaccccgtt cggcaagttc tgcggcgcgc tgtcgacggt gtcgccgacc
     1741 gacctcggca tccacgcggc acgcgcggcg atcgcggcgg ccggcgtcga tgccggcgcg
     1801 atcgaccaga ccatcgtcgc caacatcggc caggccagcg ccgacgcctt cttcctgccc
     1861 cgccatatcg gcctctacgc cggcgcgccg gaggcgagca ccgcgctgct ggtgcaacgc
     1921 atctgcggct ccgggctgga agtcctggcg caggccgcgg agcaggtcgc gctgggcaag
     1981 gcgcagttgg tgctgggggt cggcaccgac accatgagcc gcttcccgct cgcctcctac
     2041 accgcgcgcc agggcttcgc gctcggcaag ccggagttca ccgacctgtt gtgggaagcg
     2101 ctcgacgaca ccgccgcggt gccgatgggc cgcaccgccg acaacctcgc cgggcgctac
     2161 gcattgaccc gcgccgaggt cgatggcttc gcccaccaga gccaggtgcg ctgcgccgcg
     2221 gcgcgggccg ccggcttctt cgagggcgaa ctcgcgatgg tgcccggcca gggcgccttc
     2281 gaagccgcga cgctgaagcc gcgccgcttc cgctgcaagg acaaggccgg gctgggcgcc
     2341 gacgaacatc cgcgcacaac cacgccggaa gcgctggcga acttgccgca cgtgttcagc
     2401 gaagccggtc cgaccaccgc cggttccgcc tcgggcatcg tcgatggtgc cgccgcgctg
     2461 ctggtcgcat ccggcgattt cgtgcgcgcg cacggactcg ccccggtcgc gcggctggtc
     2521 gcgcacgcct cggccggggt ggcgccggcc tacatgggca tcggcccggt gccggcggtg
     2581 cgcgccaccc tcgccgccag cggcatgacg gcggacgaca tcggccgctt cgagatcaac
     2641 gaggccttcg ccgcgcagtg cctggcggtt gcgcgcgaac tcggcatcga cgaagcgcgg
     2701 ctcaacgtca acggcggcgc catcgcgatc ggccacccgc tcggcgccac cggcgtgcgc
     2761 ctggcgctga ccctggcgcg aacgctggcc cgcgacgacg ccggcttcgg catcgcctcg
     2821 gcctgcatcg gtggaggcca gggcaccgcc ctgctgctgc agcgatgctg agccgctacc
     2881 gccagcgcgt cgcgcttgtc accggcgccg gccgcggcat cggcgcggcg atcgcgcgcc
     2941 gcctgcacga tgaaggcgcc agcgtggtgc tggccgaccg cgatggcgat gccgccgccg
     3001 cgctcgccgc ggaactcggt ggcgggcgcg tgctcgcggt cgcggtggat gtcaccgcgc
     3061 gcgccggact cgaggcgctg atggagcgca tcaagagcga tctcggcggt ctcgatctgc
     3121 tggtcaacaa cgccggcatc atccgcgacg gctggctctc caggctcagc gaagccgact
     3181 gggacagcgt gctggcggtc aacctgaagg gcccgttcct gtgctgccag gccgccctgc
     3241 cgctgctgcg cgaacgcgcg ggcagcgcga tcgtcaacat cgcgtcgcgt tcgtggctgg
     3301 gcaacccggg acaggccaac tacgcggcca gcaagggcgg cgtagtcagc ctgacccgca
     3361 ccctggcact ggaactggcg cgcgacggca tccgcgtcaa cgcggtggca ccgggcctga
     3421 tcgacacccc gatgacccag gcgatgccgg gcgaggcgcg cgagcgcctg ctgcggatgc
     3481 agccaagcgg gcagatgggc gcgcccgccg acatcgccgc ggcggtcgcc ttcctcggct
     3541 ccgccgatgc cgccttcatc agcggccagg tgctgcacgt cgacggcggc aagagctgcg
     3601 gcctgctcgg actctgacca tgaccgcgct gccgccgccc gcctcgacgc ccaccgcccg
     3661 catcgtcgcg gcgcggcgca cgccgatcgg gcgcagccat gccgacagcg gtgccctgcg
     3721 cgacatgcgc gccgacgaac tgctggcgcg gctgatcgcg gaagtgacgc gcacgctgcc
     3781 cgatgccggc gcactcgacg acgtactggt cggctgcgtc ggccaacacc tggagcaggg
     3841 caagaacatc gcgcgcctgg ccagcctgct cgccgggctg ccggccagcg tgcccggggt
     3901 caccttcaac cgcctgtgcg cctccagcct ggaagccttc aacaccgcgt ccatgcgcat
     3961 cgctggtggc caggaccagc tgctgctggc cggcggcatc gaacacatgc agcacgtgcc
     4021 gatggcggcg gcggtcgact accaccccga cctgctggcg cggccgccat tcccgttcaa
     4081 caacatgggc ctggccgccg aatggctggc cgtgcgcgat ggcatcggcc gtgccaccca
     4141 ggacgcctgg gccctggaaa gccaccgccg cgcctgccac gcacgcgcgc acggccgctt
     4201 ccggcgcgag atgctggcaa tcgacggcat cgccgacgac cagtgcccgc gcgcggatgc
     4261 cagccgcgac gcgctggccg cgctcaggcc ggccttccgc gaagacggca gcgtcaccgc
     4321 cggcaatgcc tcgccgctgt ccgacggcgc cagcctggtc ctgctcgcct cggcgcaggc
     4381 ctgcgtgcgc gacggcctgc gcgcgcgcgc ggaggtgctg ggcagcgtgg tggtcgggct
     4441 ggcgccggag gcgatgggcg acggcccggt gctggcgatc cgcaagctgt tggcgcgctt
     4501 cgggctggcc acgggcgaca tcgaccgcta cgagatcaac gaggccttcg cggcgcaggt
     4561 ggtctccaac gtacgcgcgc tcgcgctggt ggccgaacgc gtcaatccgg atggcggcgc
     4621 gatcgcgctt ggccatccgc tgggggccac cggcacccgc ctggtcacca ccctgctgca
     4681 cggcctcgaa gccgacgatg gcgagctcgg catcgccgcg ttgtgcgttg gccacggcca
     4741 gggcgtggcc accctgatcc ggagactgca ctgatggatg ccgctgcgcc gaccagcccc
     4801 accgccaccg aaaccctggc gctgcaggtc aacggccagc gccacgtgcg cgcggtgccc
     4861 cccggcacca gcgtgctcga cttcctgcgc gaacagctcg gcctgaccgg cagcaagcgt
     4921 ggctgcgacc ttggcgaatg cggctgttgc gcggtgctgg tcgacggccg gccgatgctg
     4981 tcctgcctgc tgccggcggc ggacatggtg gaggccgaaa tcaccaccat cgagggcatc
     5041 ggctgcggca acgccctgca tccgctgcag caggcgatga tcgaccacgg cgcgatccag
     5101 tgcggctact gcacgccggc aatggtgctg aacggggtcg ccctgttcgc cgccgacccg
     5161 cacccggacg aacgcgcgct gcagaccgcg atcgccgcca ccacctgccg ctgcaccggc
     5221 tacaacgcca tccgcaacgc cttccgcgcg gtcgccgccg gcggggaggc aggatgaagc
     5281 cgtaccgcat cgtcggccag ccgcaggcgc aactcgacgc ccgcgacaag gtcaccggcc
     5341 gcgcccgcta caccgacgac ctgcgcgtgc ccaacccgct gcacgccgcg atcctgcgtt
     5401 cgccgcatgc cagtgcgcgc atcctgcgca tcgacacccg gcgcgcactg gccctgcccg
     5461 gcgtgcgcgc ggtgctcacc ggcgccgact tcgagcgcac gttcggggta ctgccgatca
     5521 gcaaggacga accggcgctg gcgcgcgagc gggtgcgcta cgtcggcgaa ccggtggccg
     5581 cggtcgcggc cgagactgcg gagatcgcgg cgcaggcact ggcgctggtc gcggtcgagt
     5641 acgcgccgac gccgccggtg ctcgacgccc gccacgcact caagcccacc gacgacccgc
     5701 tgcacccgga gctgaagcgc gcggacaacc tgcacaagca ggtcgagcag cgcttcggcg
     5761 atccggaggc ggcgttcgcg gcggcgccga tcgtgatcga gaagcgcttc cgctacgcgg
     5821 cggtcaccca cgccttcacc gaaccgcacg cggtgcaggt ggagatcgac ggccgcggca
     5881 acctcaccgt ctacagcgcc acccaggtgc cgcattacgt gcacctggcg ctggccgagg
     5941 tgctgggcat cgacgagcac cgcgtgcgcg tggtcaagcc gcatcttggc ggcggcttcg
     6001 gcggcaagag cgatccgttc ccgcacgaga tgatcgccgc caagctggcg ctggtcagcg
     6061 gccgcaacgt gcgcctgcgc ttctcgcgcg aggacgtgtt cttcagccac cacggccgcc
     6121 acccgaccca gctgacgatg aagctcggct tcgacccggc ccgcggcatc accgcgctga
     6181 ccggcgacat cctgatcgat ggcggcgcct acggcagctt cggggtggtc accacctatt
     6241 acaacggggt gctgctgcag ggcccgtacc cggtgccgaa cttcggcttc cgctgccgcc
     6301 gcctgtacac caacacgccg atgtgcggcg ccatgcgcgg ccacggcggg gtcaacccgc
     6361 gcttcgccgc cgaggtgctg atggacatgg ccctgcacca ggccgggctc gatccatgcc
     6421 agggccgcat cgacctgctg caccacgcga acacgctgac cgtcaacgaa ttccggatca
     6481 cctcggctgg cctcaagcgc ggcctcgagg aagcgatgca ccgctccggc tggcgcgaga
     6541 aacacggcaa gctgccgttc ggaaaaggca tcggcgttgc ctgcgggttc ttcatcagcg
     6601 gcagcgccct gccgatccac tggaacgagc gccccggcag cacggtgcgg atgacgctgg
     6661 agttcgacgg cgcgatcacc gtgtattcgg gtgcctccga catcggccag ggcagcgaca
     6721 ccgtgctggc gatgatggcg gcggaagtgc tgggcgtgga cctggaccag gtcaaggtgg
     6781 tcgccgccga cacccgcctg accccggtcg acctcggcag ctacagctcg cgggtcacct
     6841 tcatggccgg caatgccgcg cgcaacgcgg cgatccgcat gcgccgcctg ctggtcgccg
     6901 gcgcctgccg caagctcgga ctggacgaac ccgtttaccc ggcgccgggg ccgcccgcgg
     6961 acttcttccg ccagggccag cgcgcacccg atttcgaggc cgacgacctc ggcgcgaatc
     7021 cggattaccg cgatggcttc gcgttcggcg gcggccgcat ccgccacctg gccagcggcg
     7081 cggacgtcgc ctggctggat gcggtgcatg cggccatcga aggccccggc atcctgcaga
     7141 ccgtcggcaa ctaccgttca ccgaaactgg gcggcacgtt caagggcgcc ggcgccgggc
     7201 tgtccccggc ctacagcttc accgcgttca tcgtcgaggt cacggtcgac ccggagaccg
     7261 gccacgtccg cgtcgacaag gccacctgcg cgcacgactg cggcttcgcg ctgaacccgt
     7321 tggcggtgcg cgggcagatc gagggctgct tccacatggg catgggccag gccttgatgg
     7381 aggaagtgct gcacgagggc ggcgcggtgc agaacgccgg cttcctcgaa tacaagatcc
     7441 cgtcgccgtt cgagcagccg gagatcgaca tcgtgctgtg cgacagcgac gagaacgaag
     7501 gcccgttcgg cgccaaggaa gccggcgagg gtccgcttgc gccggtgatc ccggcgatcg
     7561 ccaatgcgat cttcgacgcg gtcggggtgc gcctggccga agtcccgctg cggcccgacc
     7621 gggtgctggc ggcgctgcgc aagcaggcgg gcgtggcatg acccccgcat tcgacctgca
     7681 gcgcccgcgc agcctggacg cactgcatgc actgctggac gggctcgcgc ggcgccagca
     7741 gcgctttgcg atcagcgccg gcggcaccga cctggtgccg caactcaagc ttggcctggc
     7801 cacgccggag gtgctggtgt cgctgggtgc gatcccggcc ctgcgcggga tcacgcgcga
     7861 gccggacggc cgggtgcgga tcggcgcgat gacgccgctg gccacgatca ttgccgaccc
     7921 gctgctgcat gcggtcgcgc cggtgctggg cgaggccgcc gcgcgcgtgg cctcggcgcc
     7981 gatccgcgag cgcgccaccc tgggcggcaa cgtgctggcc gaccaccgct gcaacttctt
     8041 caaccagagc ccgctcaacc gcgccgcgca tggcccctgc ttcaaggccg gcggcgacgt
     8101 ctgccacatc gtcaagtcgg cggtgcgcgg ccagctgccg caatgccagg cacgcatcgt
     8161 ctccgacacc acgccggccc tgctggtgct ggatgccgaa ctgcagatgc actcgccgcg
     8221 cggcgaacgc cgcatcgcac tggcggactt ccatcttggc gacggcatcg aacgccaccg
     8281 gctggccgcc gacgaggtgc tgctggcgat cacgctgcct tcgcggcccg ggcgggtcgc
     8341 cgcgtacgaa aaactggccg cgcgcgccaa cctcgacttc ccctcgctgg gcatcgccct
     8401 cgcccgcgac ggcgaccgtc tgcacgcggc gatgaccggc atcggcgtgc gcccggccag
     8461 cgcgcgcgcg gcgcttgcgg acggcgcgct ggcgacgctt ggcgaaagcc tcgccagcgc
     8521 caatccggtg tacgaccagg acggattccc gcgcgattac cgcaagcgca tgatcgcggc
     8581 atacgtgcgc aaggccgcgc gccggatcgg aggtgatgca tggacgtgaa acaggtgatg
     8641 cgcgagtact tcctggacct cgatgccgcg gcgaaggatc cggcgcgcaa ggtcgcgtgg
     8701 tgcacctcgg tcggtcccgc cgaaatcctg cgtgcgatgg gcttcgcggt gtatttcccg
     8761 gagaaccacg gcgccatgct cggcgccacc cgcgtcgccg gcgacaccat cccggtcgcc
     8821 aacgcgaacg gctattcgcc ggagatctgc tcctacctga ccgccgacgt cggcgcctgc
     8881 atgaagggca tcaccccact gacccgcgcc tacgggatcg aggcggtgcc gcgccccgac
     8941 gtgctggtct actgcaccaa ccagtgcaag gacgtgatgc actggttcgc ctggtacgcg
     9001 cggcagtgga acgtgccatt gcttggcgtg cacacgccga accacctgcc cgaggtcgag
     9061 cccgagcaca tcgccgatgt cgccgcccag taccggcggc tgatcacgca gcttgaggcg
     9121 gtcaccggcg agcgcctgga cccgcaggcg ctgcggcagg cggtggcgcg ctcgaagcag
     9181 gcgaccgacc tgtgggccga ggtactggcc accgcgatgg cgcgcccggc accgctgagc
     9241 ttcttcgatg gcgcggtcta catggggccg atcgtggtcc tgcgcggcac ccaggtggcc
     9301 accgattact accgcgcgct gctcgacgag atgcgcgcgc gcgtcgccgc gtcccgggcc
     9361 gcggtgcccg gcgaacgcct gcggctgtac tgggaaggga tgccggtgtg gggccggctg
     9421 cggcagctgt ccgacctgct ggcgcgcaac cgcacctgcg tggtcgcctc gacgtattgc
     9481 aactcctgga tcttctccga cttcgacccg gatgcaccgc tggagagcat ggcgcgcgcc
     9541 tacctgtcga tcttcatcaa ccgcgacgac gcctacaagg aacgctacat cgcgcggctg
     9601 gcggaggaat tcggcatcga cggggtggtc taccacgccg cccgcacctg ccccaacaac
     9661 accaacagcc actacaacct gccgcggcgc ctgcaggccg ccggggtgcc ggggatcgtg
     9721 atggacggcg acctcaacga cctgcgctgc ttctccgacg agcagagcac caccctgctc
     9781 gaagccttca tggagaccct gtgagcaccg cgcttgccag ccatgccgaa gccgcggccg
     9841 cgcccgcgga tgccagtcca cgcgtctact ggggcgtgga ttgcggcagc gccgagatca
     9901 aggtggccgc ggtcgacgcc gatggccagg tgctgctgac ccgcaagcag cgcaccctgt
     9961 tcccgctggc cgagcacgtc caccgcgcgc tggccggcgc ccccggcgcg gcgtcgccgc
    10021 tgcatgagga cgaccgcgcg gcgttgccgg aaggcggcgt gccgcggctg cgcgcgggcc
    10081 agcgggtgta cgccaccggc tacgggcgca accacatccc gttcgtgcat ggcgcctgca
    10141 ccgagatcaa ggcgcattac ctgggcgtgc agcaccagct cggcctggcc gaggactaca
    10201 cgatcatcga catcggcggc caggacgcca aggtgatcgg cgccaccccg agccgggtcg
    10261 gccagttcgc gatcaaccgc aagtgcgcgg ccggcaccgg tgcctacatc gaggaactgg
    10321 cgcaccgcct ggaggtgccg atgcagtcgc tgccggcgct ggcggccgcg cacgaccagg
    10381 agctcaccct caacagctac tgcacggtgt tctccgggca ggaagtgatc aagctgctga
    10441 tgcacggcga gcgggtcgag aacctggtgc acgcgctgta ccgctcggtg gtcaagcggg
    10501 tgctggagat gaccgcgatc gataccccca cgctggtgtt ctccggcggg gtgatggccc
    10561 accaccagaa cctgatgccg ctgttcggcg aacgcttcga acgcggcgtg cacaccatcc
    10621 ttgccccgaa tgcgcagttc tgcggcgcga tcggggccgc gatccacggc tacaacgagg
    10681 agaagaaaga tggctttccg catcaccagt gagtgcaacc tgtgcgacgc ctgcgtgccc
    10741 gaatgcccga acgaggcgat caccgccggc agccgcatct acgtgatcga cccgatgaag
    10801 tgcaccgagt gcgtcggctt cttcgacgag gcgcagtgcg cggcggtgtg cccgcaggaa
    10861 tgctgcgagc cggatccgga ccgccccgag gacaaccagg cgctgctgca gcgcgcgcgg
    10921 gcgctgcacc cggacgagga gttcacggac gacgcgccct cgcgccacgc aaccaactga
    10981 ccacccgcgc ccggagcatc cgatgaacca ggccaccgcc agccccgccg cgatcgaacc
    11041 cgacgacgcc tgcctgctcg ccgagatcac cctgaagttc gccggcgact ccggcgacgg
    11101 catccagctg gccgggcagc agttcgcgca actggccagc gccgccggcg agcgggtgcg
    11161 caccctgccc gatttcccgg cggagatccg ctcgccggtg ggtaccctgg gcggggtctc
    11221 gggcttccag gtgcgcgccg gcggcgaaca ggtgctcacc cccggcgacc gcctcgacct
    11281 gctggtggcg atgaaccccg ccgcgctgcg cagcaacctc gacaagctca agcccggcgc
    11341 gatcgtgctg gtcaacgagg atgccttcgg cgccgccaac ctgggcaagg ccgggttcgc
    11401 cgccaacccg ctggaagacg gctcgctgcg cgcgtaccgg gtgttctcgg tgccgatggg
    11461 catgctcacc cgcaacgcgc tgaaggggct gccgctgtcc acccgcgaga aggaccgcag
    11521 ccgcaatttc ttcgcgctgg gcctgatctg ctggctgttc ccgcgcccgc tggaggacgt
    11581 cgaggccttc atcgagcgcc gcttcgccgc caacccggaa ctggccgaag ccaaccgccg
    11641 tgccttccgc accggctgga actatggcga gaccgcggag ttcttcgtca gcccgctgcg
    11701 ggtggccggc agcgagcggc tgcgcgaacc cggggtgtac cgcttcgtca ccggcaatgc
    11761 cgcggtcgcc aagggcatgg tcgccgccgc ccgccgcgcg ggcctgccgc tgctgctggg
    11821 cggctacccg atcaccccgg ccaccgaggt gatgcaggaa ctcgcgcgcc tgcgcgagcc
    11881 ggacgtgcgc atgttccagg ccgaggacga gatcgccgcg atcggcagcg cgatcggcgc
    11941 cagcttcgcc ggcgcgctgg gcgccaccag cacctccggg cccgggctgt cgctgatggg
    12001 cgaattcctc aacctcgcgg tgatcgcgga gctgccactg gtggtggtcg acgtgatgcg
    12061 cgccggtccc tccaccggga tcccgaccaa gaccgagcag gccgacctgc tgcaggccct
    12121 gcacggccgc cacggtgaat cgccgatgcc ggtgctggcc tgcgccagcc cggcggactg
    12181 ctaccacgcc accgtcgagg cctgccggat cgcgctgcgt tacatgaccc cggtgatcgt
    12241 gctgtccgac ctctcgctgt ccggcagcgc cgaggtctgg cgcgagccgc acgtcgacga
    12301 actggcgccg ttccatgtgc accgggtgac cgacccggac ggtttccagc cctaccggcg
    12361 cgacccggaa acgctggcac ggccgtgggc gctgcccggc accccggggc ttgaacacgt
    12421 gatcggcggc ctggagaagg ccggcgacag cggcgcagtc tgctacgagc cggaaaacca
    12481 cgaggcgatg gtgcaactgc gcgcggacaa gatcgaccgc atcgcccagg actatcccgc
    12541 caccgagatc catggcgacc cggatgcccg cttgctgctg ctgggctggg gcagcaccca
    12601 cggcgccatc cgcgaggcca ccgaccagct gcgcgccgcc gggcggccgg tcgcctgcct
    12661 gcacctgcgc cacctcaacc cgctgccgcg cgaccttgcc gaggtgctgc gccggcaccc
    12721 gcaggtggcc gtgctggaga acaactccgg ccagctgtgg cggcacctgc gcgccagctg
    12781 cctgctcgac ctcaagcgcc tgggcaaggt ccagggcacc gccttccacg tgccggaaat
    12841 catcgcctac gccgaatcgc tgctggagac gcccgcatga gcgagaccgc caccgccctc
    12901 gccgaacagg cggacgaccg ccgccaggac ttcctgtccg cgaccacccc gcgctggtgc
    12961 ccgggttgcg gctgcttcgc ggtgctgaaa tcgctgaccg cgacctatgc ggcgctgggc
    13021 atcccgcgcg agaagctgct gaccatttcc gggatcggct gcacctcgcg cctgccctac
    13081 tacacccgca ccttcggctt ccataccgtg cacgggcgcg cgccgacggt ggcgatgggg
    13141 gccaagctgg ccaacccgga gctgtcggtg tggctgatct ccggcgatgg cgatgcgatg
    13201 gcgatcggcg gcaaccacac catgcacctg ctgcggcgca atcccgacat caagatgctg
    13261 atgctcaaca accagatcta cgggctgacc aagggccagg catcgccgac cagcccggcg
    13321 ggactggaaa ccaagtcgac tccgttcggc gtgcaggaca acccgatcaa cccggtcaac
    13381 ctggcgctgg cctgcggcgc caccttcgtc gcccgcgtcc cggacaccaa caacgcgatg
    13441 atggaagaag tgttcgcggc cgcgcacgcg caccgcgggg tcgcgttcgt cgaggtcctg
    13501 ctgaactgcg tcaccttcca cgacggcgcc tacgaacaga tcaccagcaa ggccacccgc
    13561 gacgaccaca gcgtgcgcct gcgcgcgggc cagcccctgc gctacggcaa ggacggcaac
    13621 cgggtgctgg tgcatgcgca cggccggatc gaggcgcagg tgctggaaga cggccaaccg
    13681 ctgcccgcca acgcggtggt ccacgacccc accgatcccg gcctggccca gcaactggcg
    13741 ttgatggcgc tgccggagct gccctgcgcg ctcgggatct tccgccaggt gcaggcgccg
    13801 gtgtacggtc aggcctgctg acttgcacga gggagccatg gactcgctgt acgtccaccg
    13861 cgcgccggtg cgtttcaccc acaccgatcc ggccggctac gtgttcttcc cgcgcttctt
    13921 cgagatgttc caggccgcgg tcgaggactg gttcacccac tgcctgggcc tgaactacgc
    13981 cgagcagatc ctccgccacg gcatcggctt cccgaccgcg cgcaccgaat gcgatttcat
    14041 gcgcccgtgc cggctcggcg aggtgctgga actgcggctg gtgctggagc aggtcgggcg
    14101 cagttcgatg accctgcgct tcgatggcag cgtgcacggc gaggcgcggt tgcgcgcgcg
    14161 ctcggtcctg gtcgcgatcg aaatgcagca tggccgcccg cagccgatcg ccagcgtctt
    14221 gcgcgagcgc ctgcaggcct acctcgcgct gtgcgaggcg catgcctgaa ctggccgccc
    14281 tggtgctggc ggccggtgcc ggccgtcgct tcggtgcgat caagccgctg gccgaggtgc
    14341 gcgggcaacc gctggtgcgc cacgccatcg cgcttgccga cgcggtgcag ccgtgttcgc
    14401 tgcaggtggt gctgggcgcg cagcacgaag cgatcgccgc gcggctgccg gattcggccg
    14461 gggtgatcgt gcacgaaggc tggcagcgcg gactgggcag cagcatcgcc gccggcatcc
    14521 gcggccttgc cggccagccg gatggcgtcc tgctgctgct ggtcgaccag gtggcgctgc
    14581 gcacgcaggc gctgcaggcg ctggccagcc gctggcgcgc caccccggag gcggtggcct
    14641 gcgccgacta cgatggcctg ccgggggttc ccgcgatcct gccgcggcgg ctgttcgccg
    14701 cgctggaagc gcttgatggc gaacgcgggg ccaagccgct gttgctggcc gaaggcgatg
    14761 cggcgctccg gctgccgatg ccggaagcgg cgatcgacat cgatacaccc accgacctgg
    14821 cgcgctggct ggccacctcg cccgactgaa gcggctggct cagccgccgg cctcgatccg
    14881 ccggcattcg cgctggtaca gcagccccgc ctgcagcagg ttcatgccga gcagggcgaa
    14941 gctggcggcc cacagcagcg ccgccgggcg caccagcacc ggaagccaga ccgccgccag
    15001 cagcagcgcc agcgccagtg cgtggcattg cagctggcgc cggcaacgcg cttccggaag
    15061 gatgctgcgc atgttcggca gtaccagctg gtggcgcagg tcgagccggc tggacaggcg
    15121 ctgctgccag tgcagccaga ccaggaacgg cacgatcttg tagagcatcc cgctggccac
    15181 cgacagcgcg aaccccgcca ggaacagcac cgtggcggtc accagcaggc gctcggcgac
    15241 tgcacccggc agccaccagg ccagcatcgc cgccagcgcc gccagcagca gcatcgccat
    15301 ccccagccgc cagtaggcca ggctgctgtc gccgagcttg cgccggcgct gcccgagcag
    15361 gcgcagggtg gtcaacccaa agccgcccag caggaccgcc agcagcagcc acggcagcca
    15421 cggcagcggc cagcccagcc acttggccag cgccagccag gccagcagcg caaacgtcgc
    15481 gggtggcagt acccgccgca gcgcggccgg gtacgccggg gtcatctgga acatcggcac
    15541 cacctgccag gcaaccaccg cgaccagggt cgcgatccag ccaaccaggc ccaccgccag
    15601 gtgcaggtcg gtcagcggcc agcgccacaa atccaggccc aggccggcat ggcccagcgc
    15661 cagccgcagc cccaacgcca gcaccagcag caggccaagc gcggcccagc gcatcccgcg
    15721 cacgctgtcc tggatattcg gcgcgcgtgc caggccccag cccagcgcca gcaggaagcc
    15781 gcccagcccg agccccagca gcaccgccgc cagccggaat gccagcgcgc ccgcgccgag
    15841 gaacccggct gccaacgcca gcgtgccggt caccagcagc ccgtggcagg cccccgcaag
    15901 cagccgcggc gaggccacgc gcacgcccac cagcaccggc agcatctgca gcagcgcccc
    15961 gcacatcacc atcgacagca ccccgagcgc cagcaggtgg ctcaacgcca gcgccgcatc
    16021 ggtccagcgc gaggccagcg cctgcggccc ggcccacccc agcagggccg ccgccagtac
    16081 cccgaacagc ggcgcggtca ggaagaaccg caacggcacc gagaacggcg gcgcctgctc
    16141 cagcgacagc agcgcgctca tccgtcgcgg tccgccacgg ctccatcggc ctcgccggcc
    16201 ggccacacca gcagctcgca gcggtcgtcg gtcacctcca gcacgcgcca ggccagcccg
    16261 cgccgggcga ggatcgggaa cagcggcagc ggctcgcggc tgagcagcgc gcgcaggcgc
    16321 tggcccggct gcagcgcatc gcaggcggcc aggatccgct ccatcggctc gcatggcggc
    16381 agcccgcgca cgtcgatcgc acgctcgccg ccggacgcgg ccatcagtgc ccctgcaggc
    16441 gctgcagcag ctcgtcggcc tggtcgccga gggcgcggtc gcacatcggg tagagcatct
    16501 gctcctcctt catgttgtgc tgctggacca ggacgttgag ggtttcgccg aggccgagga
    16561 aatcgtccag gctgccggca tcgagcgcgc gctgcagttg ctccagcagg gtgcgcatct
    16621 gcacgtgctc catccgcatc accgcggtcg gcccgccggc catgccggtg cgcgcctcga
    16681 acgccgggaa cagcacctct tcctcgatcg ccaggtgctg gcgcagggat tcggcgaagg
    16741 cggcgaatgc ggatcccgcg gcggccaggt cgccctcgtg ggcggcgttc tcggcatcgg
    16801 cgaacagcgc atcgcagtcg cggtgttcgt tggtcaggaa gtcggtcagc atgcgggcgg
    16861 ctcgtggctg gggtgtggcc gcgactctgc cacggccgcc gcggcatggc cttgctcccc
    16921 gtcaagtgcg cacgccagcg cctgcagccg cgccgccagc gacagcgcga tcgcggccgg
    16981 cccgcgacca cccagcgcca tcccggcggg cgattccagc ctcccgtgca ggcgcgcccg
    17041 gaccgccggc ggcagcgtcg caagcaggtc gtcgcggcgg gtttccgggc caagcaggcc
    17101 gatgtaggcc ggcagccgcg gccattcggc cagcgcctcc agcgcggcgc ggtcgcggtc
    17161 gaactgatgg gccatcagca acaccccatc gtggccccgg tcctcgccgg cgcccggcag
    17221 cgccgccagc tgccgctcca cccattgccc gcaatgcagc catgccgggc gcgactcgac
    17281 cagctccacc cgccagccca attgggccag cagcggcagc agcagttcgc tctccggccc
    17341 cgcgccgcaa gccagcaggc gtggcagcgg cgccaggcgc aagcgccagg cctgcgcagg
    17401 tgcgccagcg gcggcatccg ccagcgccag cggccattcc ccatgcaggt cgccgcaggt
    17461 cgccgacagg gtgcccggca gggtcaccgc caacgccagt ggatcaccgg cgtggcgata
    17521 gcgggcaagg atcggctgca gcgcgggcag ctccgccagt ggcagcagca ggatccgttg
    17581 gtggccgcgg caaccggcgc cggagccggc gaacacgtcg gcaaggtcga ggttgtccag
    17641 ttccagcagg cgcggccgca ggtcctggcc agatgccgcc gccgcttcca gcagcgcgcc
    17701 ttccaggcag ccggggctga tccagccgca gcgctcgccg ccggcaccga tgtggaccac
    17761 gctgccggcc ttggcatagg catggccggc aacctccagc accagtgcca gcacgcccgg
    17821 ccgcggccgc gcgaaatccg cgaggcacgc atccagcgcc gccttcgcag gtgcgccggg
    17881 aacattcctg tacacgttgg cggcgtgcac gcggggaccc gttcagccct gcaggggctc
    17941 gaagcgcgcg gtgaagtggc gcagcgccgc tggctcttcg gtgatccggt agccacccag
    18001 gccggcggac ctggccgcga cctcggcgca gacctcgatc acgtagtcga tgtggctttg
    18061 ggtgtaggtg cggcgcggga tcgccagccg caccaggtcg agcgcggcgg gctgttcgct
    18121 gccgtccggg cggcggccga acatcaccgt gccgatctcg cagctgcgga tgccgccggc
    18181 ttcgtaaagc gccaccgcca acgcctgccc cgggtactgc aacggcggga tgtgcggcag
    18241 catccggcgc gcatcgatgt acaccgcatg gccgccgacc gggcgcatgg tcgggatgcc
    18301 ggcggcgtgc aggccatcgc cgaggtagcc ggtcgagcgg atgcggtagc gcaggtagtc
    18361 gtgctgcacc acttccacca ggccctgggc gatcgcgtcg aggtcgcgcc cggcaaggcc
    18421 gccgtaggtc gggaagcctt cggtcaggat cagcaggttg cggcaactgg cggccagcgc
    18481 gtcgtcgtgc atcgccagcc agccgccgat gttggccagg ccatccttct tcgcgctcat
    18541 ggtcatgcca tcggccagcg aggcgatctc gcgcacgatc tcggcgacct cgcgcccgcc
    18601 ctgccccggc tcgcgctcgc ggatgaacca ggcgttctcg gcgaagcggc aggcatccag
    18661 gaacagcggc acctggtggc ggtcgcacag cgcgcgcacg ccgcgcaggt tctccagcga
    18721 taccggctgg ccgccgccgg aattgttggt gatggtgacg aacaccgccg ggatgtcggc
    18781 gcggcgctcg cgcagcagcg cgtccagccg cgcgaggtcc atgttgccct tgaacgggtg
    18841 ttccagcgcc gggtccaggc cttccgggat caccaggtcg acggcctcgg caccggtggc
    18901 ctcgatgttg gcgcgggtgg tgtcgaaatg ggtgttgttg gggatgatcc ggccagggcc
    18961 accgatcacc gccatcagga tcttctcggc ggcgcggccc tggtgggtgg ggatcacgtg
    19021 gcggaacggg aacagcgcct tcaccgccgc ctcgaagcgg taccacgagg gcgagccggc
    19081 ataggcctcg ttgccgcgct ggatgccggc ccactgctcg ctggacatcg cgccggtgcc
    19141 ggaatcggtg agcaggtcga tcagcacgtc gtcgctgtgc acggcgaaca ggttgtagcc
    19201 ggcggccgcg atcgccgcat ggcgttcctc cgcggtggtg atgcgcagcg gctcgaccga
    19261 gtggatgcgg aacggttcga tgatggtggg catgtcaggg ctgccgatgg ggatgggtgg
    19321 gcgggccgag ggcggtaccc gcggcggatc ggccggcaac cgccggcgca atgctcacag
    19381 caggtgccgg agcacggcga cgtagtggca gacgctgccg gcgatcacga agccgtgcca
    19441 gatcgcgtgc gaccagcgca gcccgggacg catgtagaac accgtgccga gggtgtaggc
    19501 gatgccgccc gcggccagcc aggccagggt tgcgccatcc agcgcgtgcg ccagcggctt
    19561 ggccgcgacc acgatcagcc agcccatcgc caggtagatc agggtcgaca gccgctggaa
    19621 gcggccggtg tagaacagct tgaacaccac ccccgccagc gcaagggtcc agatcgtgcc
    19681 gaacagccac cagccgatcg atccgcgcag gccaaccagg gtgaacggcg tgtaggttcc
    19741 ggcgatcagc aggtagatcg cgcagtggtc gaacaccttc aaccgcccct tcaccaccgg
    19801 gtgcgaaatc gcgtggtaga gggtcgaggc ggtgtacagc agcagcagcg cgaccccgaa
    19861 cacgatcgcc ccggcaagct gccagccatc gccgtaaacc gccgccaggg tcaccagcac
    19921 cgcaccggcg gccagcgccg ccgccgcacc gacgccgtgg gtcagcgcgt tggccagctc
    19981 ctcgcgcgcg tggtgcgcgc gcgggtgcgg ggacggggcg gagccgctgg cctgcatgcg
    20041 ggtggcctcc cgctcaggcg aacgggtcgc gcaggacgat gttggcgtcg cggtccgggc
    20101 cggtgctgac gatcgccagc ggacagcccg ccagttcctc cagcgcgcgc aggtaggcgc
    20161 gcgcggcggg cggcagcttg tcccagtcgg tgatgccggc agtggactcg ctccagcccg
    20221 ggaattccag gtagaccggc gtgcactcgt cccagccatc cgcgtccagc ggcgcgtatt
    20281 cggtgcgctt gccgcggtat tcgtaggcga tgcagatgtt gagcttctcc atcccgtcga
    20341 ggatgtcgag cttggtgatg cacaggccgc tgatgccgtt gatcgccacc gcgcgcttga
    20401 gcgccacgat gtccatccag ccgcagcggc gcgggcggcc ggtggtggcg ccgtattcct
    20461 ggccgcggtc gcggatgccc tggccgatgg cgtcgtccag ctcggtcggg aacgggccgc
    20521 caccgacgcg ggtggcatag gccttggcga tgccgagcac gtagtcgatc gcgtccgcgc
    20581 ccacgccggt gccggcgagc gcgccgccga cggtggtgtt cgacgaggtc acgtagggat
    20641 aggtgccgtg gtcgatgtcc agcagcgagc cctgcgcgcc ttcgaacagc accttcttcc
    20701 cggccttgcg caggtcgtgc aggatgccgg ccacgtcggc cttcatcggc tcgacgtact
    20761 cgccgaaggc cagcgcctcg tccagggtct gctggtagtc caccgcatcg accttcaggt
    20821 actgggtcag cacgaagttg tggtaatcca gcgccacgcg cagcttctcg gccagctcgt
    20881 tcgggtagtg caggtcggcc acgcggatgc cgcggcgcgc caccttgtcc tcgtacgcag
    20941 gtccgatgcc gcggccggtg gtgccgatcg ccgacttgcc ggcggccttc tcgcgcgcct
    21001 ggtccagggc gatgtggtac ggcatgatca gcggcgtcgc cgggctgatc ttcaggcggc
    21061 tgcgcacttc cacgccgttg ccctcgagct cggcgatttc cttctgcagc gctcccgggt
    21121 gcaggaccac gccgttgccg atcaggcaca gcgcgtcgtc gcgcaggatg ccggacggga
    21181 tcaggtgcaa cacggtcttc ttgccggcta tcaccagcgt gtggccggcg ttgtggccgc
    21241 cctggaagcg caccaccgcg ccgatgtcct gcgtcagcag gtcgacgatc ttgcccttgc
    21301 cttcatcgcc ccattgcgcg ccgagtacga cgactgactg acccatttgc gtgctccaga
    21361 cgtgagaaaa gccgaggcgt gccccggctt tccggcattc tacggggttc cggattcgcc
    21421 cgcgagcatc agccgccgcg caccagccac agcgagacga tccccaggat cagcacgatc
    21481 ccgccaaccc ggcgcacgtg ctcgtccggc agttgctgca gctgctccgc ggcgcgcttc
    21541 caggcgccgg gggcggcgaa caggaacagc ccttccagca ccgccaccag gcacagtgcc
    21601 gcccacaggt cggacatgct cagcggtcgc tgcgcatgta ctgcaggaac gggtcgtcgc
    21661 gttccagcac gatcacgctg tcgccgccgg cgaaggccgc gcgataggct tccaggctgc
    21721 gctggaacgc gtagaacccc ggatcgcggc tggcggcctc ggcatacagg cgcgcggctt
    21781 cggcatcacc ctcgccgcgc agcttctgcg catcgcgctc ggcctcggcc gcgatcaccg
    21841 cgcgctggcg gtccgcttcc gactggatct tctgcgacag ctcctcgcct tccgcgcgca
    21901 actggctggc gacctgcttg cgctgggcgc tcatgcgccg gtacacctgc ccgatcacct
    21961 cgccgccggt cggcaggtcg atgcgcttga tgcggatgtc gaagatcttg accccgagca
    22021 ccgccgcgcc cttgttgatc gagaccagct gcgccgcgag caccttggcg cggtcgcccg
    22081 acaccacttc ctgcagcgtg cgcgcgttga tctcgttgcg cagcgcgtcc ttgatgatcg
    22141 gggccaggcg ctgcgcggcg atcgactcgt cgccgccggt ggcgcggtag aagtcgcgca
    22201 cgttctcgat cttgccgatc gcgaagaagt cgacgctgac gtccttcttc tccgaggtca
    22261 ggtagcgctc cggctccgcc gagaggacct gcaggcggcg gtcgaacacc cgcgccgact
    22321 cgatcagcgg cagcttgaag tgcaggccgg ggccgatgtc ggtgcgcacc accttgccga
    22381 ggttgagcac gatcgcggcc tggccctcgc tgaccacgaa caccgaaccc agcagcaaca
    22441 gcaacccggc gaggatcgcc ggcacccatg cggggaattt catcgcacgt tctcctcacg
    22501 gccggcactg cggccctggc gacccgggcg cgccggatcg gcggtcgcct ccacggtcgg
    22561 cgacaccagg tcaggcagga tcgcctgcgc cggcagcgcc ggctggacct gggtggcgcc
    22621 ggccatcggc acgtagatca gctggcggcc atcgccgccg accaccttgc ggttgtccgc
    22681 cagcacccgc tgcacggttt ccagccacag ccgcttgcgg gtcacctcgg gagcggcctt
    22741 gtactgctcc accagcaggg tgaagcgctc ggcgtcgccg gtcgcgcgcg cgatcgccgc
    22801 ggtcttgtag ccttcggcct cggtgcgcac gcgcgcggcc tggccgcgcg cctcgggcac
    22861 gaccttgctg gcgtaggcct cggcctcgtt cttggcgcgg tccttgtcct gctgcgcgct
    22921 gttgacgtcg tcgaacgccg gcttcacttc ctccggcgga cgcgcgttcg gcaggttcag
    22981 ctcggtgacc accagcccgg tccggtaggt gtccatcgcc gcctgcaggc gctgcttgga
    23041 ggacaccgac agcgcgctgc gcgcgctcag cacggtatcg aggtcggagc ggccgacctg
    23101 ctcgcgcacc gcgctcaggg tcgcttcctt cagcacctcg ttggcgtcgc gggtgccgaa
    23161 caggtacagc tgggcatcgc cgatgcggta ctgcacgttg atttcgacgc tgacgatgtt
    23221 ctcgtcgcgg gtcagcaccg gcaccgtttc gctgtaggtc gagatctggg tggcgttgac
    23281 cttgatcacc cgctccagcg gccacggcag cttgaggtgc gggcccgggg tcatggtgcg
    23341 gtggaattcg ccgaagcgca gcaccacgcc gcgctgctgc tcggtgacca gcacgaagca
    23401 gttgaacgcc agccacaggc caagcacgac ccacagccag cgcagcgcgc cgccatcgcc
    23461 gaagccgccg ccgaacccgg gcagccggtc cagcaagccg ccgatgccgc cgccgcgctt
    23521 gccgcgattg ttgtttccgg acgaattgcc gggtgtgttc caggccatgc ctgctccgtt
    23581 gcgccgcacg atgcggccat gattgcggtt tcgattctac cggctgcgcc ggccggggcg
    23641 tcagctttcc agcgcttccg gctcggcttc gggcagcagc ggccgcagcg gcgcgccatc
    23701 agcctgggca gcgagccgcg cggcatccgc caccggcagg tccacccgca ggcgccagcc
    23761 gtcgccgtcg tgctcctcgc ccagcaccgc gtccagcgcg tgcaggcgcg cgcgcaggcg
    23821 gccggcatcg gtgggcagga cgatctcgcc gaccacccgc tgcaggccca gcttcccgcc
    23881 cagcgcttcg cgcagcaggt cgatgccgag gccgtcacgc gcggacaccc acaccgcgtg
    23941 gccgtcctcg ccgcgctggt cgatgcgcgg ggcggcgccc tcgatgcggt cgatcttgtt
    24001 gaacaccagc aactgcggga tcccgccggc accgatctcg cgcaggacct cgtccacctg
    24061 cgccatgcgc tcgtcgcgca gcgggtcgtc ggcgtcgacc acgtgcagca gcaggtcggc
    24121 ctcgcgcgcc tcgctcaggg tcgaacggaa tgcagccacc agttcgtgcg gcaggtcgcg
    24181 gacgaagccg acggtatcgg ccagcaccac cccgccgccg gaaagctcga tccggcgcac
    24241 ggtgggatcc agggtcgcga acaacttgtc ggcggcgtag gcactggcgc cggtcagggc
    24301 gttgaacagg gtcgacttgc cggcattggt gtagcccacc agcgccaccc gcggcaactc
    24361 gctgcgaacg cgcgcgcggc gcatctgggt gtgctgcacc tcgaccttgt ccagccgctt
    24421 cgaaagcatg tcgacccgct tctgcagcag ccggcggtcg gtttccagct gggtttcgcc
    24481 gggaccgcgc aggccgatcg agccgccgcg ctggcgctcc aggtgggtcc agccgcgcac
    24541 cagccgggtg gccatgtgct tgagctgcgc cagctcgacc tgcagcttgc cttcggcact
    24601 gcgcgcgcgc tgcgagaaga tgtccaggat caggccggtg cggtccacca cccggcgttc
    24661 cagcgcgcgc tccaggttgc gttcctgcac cgggctgagc aggtggttga ccagcaccag
    24721 gtcggcgccg gtcgcctcgc aggcggcctt cacttcctcc agcttgccgc tgccgatcag
    24781 cgtggccggg ttcgggcggt cgatcttcgc ggtcagcacc gcggccacgc tggcgcctgc
    24841 ggagcgggca agctcggcga attcctccag cacgccctca ccgggcgggc caccggcgtg
    24901 cggctggatc agcagggcgt gctcgccgcg gcgcgagcgt tcgaacagtt gggttggcat
    24961 cgcaacccag atgcgggcgc cgggcgccga ttgcaagccc gcggcgcgga ccttgcccgt
    25021 gccggggtca ggcctcgtcg ccgccctcgt cgtcgccgtc ggcaccggtc gggcccaccc
    25081 gcacgttgcg cgccgggacc acggtcgaga tcgcatgctt gtagaccatc tggctgacgg
    25141 tgttgcgcag caggaccacg aactggtcga acgattcgat cgtgccctgc agcttgatgc
    25201 cgttgaccag gtagatcgag accggcacgc gctcgcgccg caatgcgttc aggaaaggat
    25261 cctgcaaaga ctgccccttg gacatgtgtt tccccactgc tgccgatgtt cttgtttgtc
    25321 gtcgtgggcc ggatgccgcc cgtgcattcc cccggccccg cgcccgccac gtttccggga
    25381 gcgcagcccg atggtagcgc aggtcggcgc aagcgcgcgc atcacccgcc gaggaatccg
    25441 gcgaccgcgg cgtccaatcg cgtgcgctgg gtcatcggat cgaacctgcg tgcgtccagc
    25501 tcgccgcgca accaggtgaa ctggcgcttg gccagttgcc gggtggcggc gatcgcgcgc
    25561 tcgcggaatt ccgctggcgt cgattcaccg cccaggaatt cccaggcctg gcggtagccc
    25621 accgcgcgca gcgccggcag gtcgagcgga tgcggatgcg ccgccagttc gggcgatgcg
    25681 cgcaacgcac gcacctcatc gaggaagccg gcggccagca tcgcgtcgaa gcgctcggcg
    25741 atgcgttcgt gcagcaggtt gcgatcgggc ggtgccagca ccagcttcag caccttcagc
    25801 ggcaagcggg cgcgctgcgc ctgccgttgc cacccactga tcggcctgcc gctcagccga
    25861 tacacctcca gcgcgcgctg gatgcgctgc gcgtcggtcg catggatgcg cgccgccgcc
    25921 actgggtcga cctgcgccag ttgcgcatgc agcgccgccc agccgcgttc cgcggcttcg
    25981 gcggtgatgg ccgcgcgcgt cgcctcatcg gcttccggca tgtcggccag gccctcgagc
    26041 aacgcacgga aatacaggcc ggtcccgccg gcgaggatcg gcagcctgcc gcgcgcgatg
    26101 atgccgtcga tcgcgccgcg cgcatccacg gcgaactccg ccgccgaata cggctgccag
    26161 ggatcgcgca ggtcgaccag gtggtgcggc acctgcgcca gttcgtggcg cgtgggcttg
    26221 gccgcgccga tgtcgaggcc gcggtagacc agcgccgaat cgacgctgac gatttccccg
    26281 cccagccgct gcgccagctc cagcgcaacc gcgctcttgc ccgacgcggt gggacccatc
    26341 agcgcgatcg cgcgcgggcg gttgtccagg gtcatcgcgg cgcggtcgga ggacggctca
    26401 atagccctgc tcgcgcaggc tcagctggac ctgctggtgg cgcgcctgca cttcggcact
    26461 cggcgcgcgc gacagcaggc tgccggcgat gatcgcgagg ctggccgcga tgacgccggg
    26521 caccatctcg tacagggcgc tgccggtctg cttccacagg atcacggtca gcgcgccaac
    26581 gatgatgccg gcaagcgcgc cctggcgggt catgcgctgc caggacagcg agaacaccac
    26641 caccggaccg aacgcggcgc cgaagccggc ccacgcatag ctcaccagcc caagcacgcg
    26701 gctgtcggga tcgcgggcga tgaagatcgc cagcagcgcc accgccagca ccgccgcgcg
    26761 gcccacccac accagttcgc gctcgcccgc gcgcgggcgc acgaacccgc ggtagaagtc
    26821 ctcggtcaat gcgctggagc agaccagcaa ctggcagctc agggtgctca tgatcgcggc
    26881 caggatcgcc gacagcagca cgccggcgat ccacgggttg aacaatgccg cggacagcgc
    26941 gatgaacacg cgctcatggt tgtcggcgac cgcgcccgcc tgctgcggat gctgcgcgaa
    27001 gtacgcgatg ccgaagaaac cggtcgccat cgccccgagc aggcacagca ccatccaggt
    27061 catgccgatg cgccgcgcct gcgggatcac cgccagcgat tcggcggcca tgaagcgcgc
    27121 caggatgtgc ggctgcccgc aatagccaag gccccacgcc agcagcgaga cgatgccgac
    27181 gaggccgagc tcgccaccct tgaaccagtc cagcttcgcc gggtcgagct gctggatcgc
    27241 gcccaggctt tcgccgaacc cgccgctgcc gatgatcacg accagcgggg tcagggtcag
    27301 cgcgaagatc atcagggtgg cctgcacggt gtcggtccag ctgaccgcca ggaagccgcc
    27361 gaccagggtg taggcgatcg tcgccgccgc gccccaccac agggcttgcg cataggaaag
    27421 gccgaacacg ctttcgaaca ggcgcgcacc ggccacgatc ccgctggcgc agtacaccgc
    27481 gaagaacacc aggatcacca gcgcggcgac gatccgcagc aacttgccat cggcggcgaa
    27541 gcgggtggtg aagtagtccg gcagggtcag cgcgttgtcg gtgcgttcgg tgtacacgcg
    27601 cagcggcccg gcgacgaacc tccagttcgc ccatgcgccg accaccaggc cgatcgcgat
    27661 ccacgcttcc gacaccccgg acaggtacag cgcgccgggc agccccatca gcagccagcc
    27721 gctcatgtcc gaggccccgg ccgaaagcgc ggtcacgtag ccgcccagcg aacgaccacc
    27781 gaggatgtag tcgtcgaaat cgcgggtgcg cttccaggcc acgaagccga tgccgagcat
    27841 cgccagcagg tagatcccga aggtgatcag cagcggcgtg ctggcggtca tgcgcgcgtt
    27901 ccccgaaaac gaaggccgca gcttagcgca gccatccgcc gcggctgcgg gcggcctggg
    27961 ctagcatggc gccatgccgt tctggaaacg cctcgggttc gcgttcgtgt tcgtcgtgcc
    28021 ggcgctgatg ccggcggcgg cgtggctggg cacgcgcggc ggccggccgg acctgatggc
    28081 gtggttcccg ctgttcttcc tgttcgtgct gctgccgcta gccgattacg cggtgggcca
    28141 cgacccgcgc aatccggctg ccgccgacgt cgccgcgctc gagcaggatc gctggttccg
    28201 cctgctcacc ctgctcgcgg tgccggtgca atacgcaacg ctggcctggt ccctgcagca
    28261 ctgggcacag gcaccgttct cgctggccgg cagcatcggc tggctggcct cgctgggcgt
    28321 ggtgggcggc gtgctggcga tcaacgtggc gcacgagctg atccacaagg acacccggct
    28381 ggagcgcgcc gccggcggcc tgctgctggc cagcgtcggc taccacggct tcaaggtcga
    28441 acacgtgcgc ggccaccacg tgcatgtctc caccccggag gatgcctcca gcgcgcgctt
    28501 tggccagtcg ctgtggcact tcctgccgcg tgccttgctg cgcaacaccg cgaatgggtg
    28561 gcggctcgaa gctgcgcgcc tggcccgcct cggcaagccg tggtggcact ggcgcaacga
    28621 gctgctgggc tggacgctgc tgtggctggc gctggcggcc gccgcgtggg cctggcttgg
    28681 caatgccggg ctggtggcct tcctggtgca gggcgtggtc gccgcctgcg cactggagat
    28741 catcaactac atcgagcact acggattgga acgcgtgcgc ctggccaacg gccgctacga
    28801 gcgcaccacc cacctgcaca gctggaactc ggactacgca ctgaccaacc tgctgctgtt
    28861 ccacctgcag cggcattccg accaccacga aaccccgcgc cgccgctacc agtccttgct
    28921 ccaccacgcg gacagtccgc agcttcccgg cggctacgca tccatgttcg tgctcgcgct
    28981 gttcccgccg ctgtggttcc gggccgtgca tccgcgcctg cacgcatggc gtgcgcgcat
    29041 cgcctcgggt ggctgagccg gccgtcgcga agaacggctt gcggcgcgcg accgcgcact
    29101 gcaggatcgc cggatccgac cgcttccacc gcagggatcc catgccagcc ctcctccgca
    29161 gcctgctgct tggcgccctg ctcctgctcg ccggctgtgc gcaattgccc gcacgcccgc
    29221 agctgcctta ccaggccgcg ctcccggtgg ccgaagggac ccggctggat gcggaggtgg
    29281 ccccggcggt ggccaggcac cccggtgcct ccgggttccg gctggtcgcc gatggcatgg
    29341 aggcgttcgc gttgcgcggc cattcggcgc gatcggccgg acgcagcctc gacatccaga
    29401 cctacatctg gcacgacgac ctcaccggcc gcctgctcgc ggccgaggtc ctggacgccg
    29461 ccgatcgcgg cgtccaggtg cgggtcctgc tggacgacct cgacgcccgc gcgaagagcc
    29521 aggcgctgct tgcgctggac agccacccgc gcatcagcat ccggatcttc aacccgctgg
    29581 ccacccgcga cggcatgctg ggtaccgcac tcgagttcgc cagcgacttc gaacgcgtca
    29641 accggcgcgc gcacaacaag tcgtggatcg tcgacggccg gctggcggtc gccggcggac
    29701 gcaacctcgg cgatgaatat ttcatggcca gcccggaggc gaatttccag gacctcgacc
    29761 tgctggtcgc cggccccgcg gtggctgcac tggcggacgc gttcgacagc tactggaact
    29821 ccgagtcggc gtggccgatt cgcgcgcttg aaccgcgcaa gccccagggg gaggcgttgc
    29881 aggcggtgcg cgagtcgctg cgccagagca ccagcgatgc cggcaaggag gcgtgggtgc
    29941 aggcgctggg cgagccggat gcactggcga ggatccgggt cggccacctc ggcatgcgat
    30001 ggacccagca atggcagctg ttgcaggacg cgccggacaa gctggtccag caggtccagc
    30061 cgatcgccgg cacggacgcg tcgccgatcc tggccggcct gcgttcggcg atgcagtcca
    30121 gccagcgcga gatcgcgatc atttcgccct atttcgtgcc cggcgaagag ggcgccaatt
    30181 cgctggtcgc gggcgtgcgt ggcggcctgc gcatccgcat cctgaccaac tcgctggcct
    30241 cgaacgacgt cgccgccgtg cacggcggtt acgttcgcca ccgcggcgtg cttgcgcgcg
    30301 gcggggtcga actgtgggaa atgaagccca ggccaggccg cgcgcagggc agcctgctcg
    30361 gctcctccgg cgcgcgcctg catggcaagg ccgcgatatt cgaccgccgg accctgttcg
    30421 tcggatcgct caacctcgat ccgcgctccg tgcagctcaa ttccgagcag ggcctgctgg
    30481 tcaccgacga ggtcctcgcg gcccagctgt cggcgatgtt cgacaacatg acccgccccg
    30541 agaacgcctg gtcgatcggc tgggaccctg cccgtggccg ggtgtcctgg agcgatggaa
    30601 acggcaccct cgatcgcgag cccgaagccg ggctgtcgcg gcgcgtggtc gcccggctgg
    30661 cctactggct gccggtcgac ccgctgctct gatcaatcct ccggccaggc gatcgccggc
    30721 ttcgcctcga tccgcggcgg cggcaccgcc gccaccgcgt tccacacctt gagcgcgtcc
    30781 gccgtcgcgg caacgtcgtg cacccgcagc agcatcgccc cgcgctgggc ggcgatcagg
    30841 tgcgccgcga ccgaacctgc gatgcgttcg cgcggatcct cgcggccggt gagctcgccg
    30901 atgcttttct tgcgcgacaa gcccgcgagc accggcacgc cgagttcggt gaagcgctgc
    30961 aactgcgcca gcagctgcag gttgtgttgc agcgtcttgc cgaagccgaa acccggatcg
    31021 accacgatgc gcttcctcgc gatgcccgcc atctccgcgg cgaagatccg ttcggccagg
    31081 aaccggtgca cctcgccgac cacgtcgtcg tagtgcggcg cggcctgcat gccgcccggc
    31141 tcgccctgca tgtgcatcag caccaccggc acccccagct gcgcggccgc gtcaagcgcg
    31201 ccttcgcggc gcagcgcatg cacgtcgttg atcatgcccg cgccggcggc gaccgccgcg
    31261 cgcatcacct ccggcttgct ggtgtcgatg ctgatcggca gcgaggtctc cttcgccagc
    31321 cgttcgatga ccgggacgac gcgacgcagt tcctcgtcga gcggaacctc ctgcgcgccg
    31381 ggccgcgtgg attctccgcc gatgtcgagg atgtcagcgc cctctgccgc cagcttcagc
    31441 ccctgcgcga tcgcggcagc ggcatcgaag tggaggccgc catcggagaa cgaatccggg
    31501 gttgcgttga cgattcccat cacctgcggg cgatcgaggc gaaggatgcg gccgttgcat
    31561 tcgagttgcg ggatggtgtc gaacatgcgg atcagatgcc cttggtctgg ctgttggcgg
    31621 gggctggcaa tccgaccttc gcgcagaacg ccgccagtcg tggatcggtc ttgtagcgca
    31681 tcaccagcgg gtcgatcagc aggttgccga tcccggggtc gcgggttgca tgcgccttct
    31741 ccagccactc gaaggcggca tccgcatcgt tgcgcaaggc ctgcacctgc gcgacctggt
    31801 acgcggccac gtcgccgtaa tcggaaatga gttcttgcaa cgcggcatcg gccgcagcgc
    31861 gatccgtgcc cgcctgcagc gccatcgcca ccgcgatatc gcgccacacg ccttcaggct
    31921 cgcgccgggc ggcatccagc gcggcctgcg catcgccacg ctgcagttcg atgatcgtca
    31981 gttgcgccca ggcggagctg ccttgcgggc gcaattcgat cgagtcgcgg atcgccttct
    32041 ccgcctcgtc caggcgaccg accgccgaca ggtacgcgct gtaccaattc caccagttcg
    32101 gcgacatcgg gtcattgtcg agcgcctgcc gcgtgagggt gatcgcctcg tccagctggc
    32161 cctgcaaggc aagcatgctg gccttgctga acatcgtctg caggttgccg ggcgacaact
    32221 cgagcccgcg ctgataggcc tcggttgcgc cgcgccaatc gagctggttt tccagcaacc
    32281 agccgcgcgc cacgtagaca tcgcccagat ccggcgccag cgtcagtgcc acgtcaccgg
    32341 ccttgcgtgc ctctgcatac gcattctgcg cttcgtcgcc actcaggccc agtgccgcca
    32401 gcgtggtcca gttgcgcgag agcgcagccc aggcgctcgc gtacttcggg tccaggcgtg
    32461 tcgcccgggt catctccgcg atggccaggc gcatgtcgcc gtcttcgccg cggtcggcgt
    32521 agaacttgcc ccgcaggtag gcggaatagg cttcgacgct gccattgggc gggcgctcgt
    32581 cctggcgctt cgcctccggg gccagcagtt tcgccttgag caccacggcg atcgcgcggg
    32641 tcagctcatc ctgcagtgcg aacaggtcgc cgtaaggctt gtcgtagaga ttcgtccaga
    32701 tcgccgaacc gtcgtcggtc cgcacgactt ccgcgcggat gcgcaccgtg ttctccgccc
    32761 gctgcacgct gcccgcaagc aggtaggcaa cgccaagctt ggcgccgatg ctgcgcgcgt
    32821 cctccttgct gtcgcggaaa cggaacgagg aggtgcgccc gatgacccgc aagccctcga
    32881 acttggagag tgcgtcgatc aggttctccg atagcccatc ggaaaagaac agctggctgg
    32941 cgtcgccgct ggcattggca agtggcagca ccgcgatcga gttgccgcgc cttgccggca
    33001 gcgccgcagc gcggacggcg gccgcgcgcg tggcttccgc gacaagtgcc tcctcgcgtc
    33061 ggggcgacag cacgaacttg tcgaacccga aataacccag cgcgagcgcc agcaacacga
    33121 tgatcgcgcg gtcgagggtg cgcgcggttc gcggcgcgat ggactccgcg gccggcacct
    33181 cggcatcccg gcgaaatccc tgcggggtca gctcgaacat ccaggccacc gccagcgccg
    33241 gcacgaaagc caccgcaagc gtcatcacca gggccttcat cgtccatggc ggcgcatcga
    33301 acacgggcag cagggtcgca ccgacctgca ccaccagcca ggccgtgacc aggtacaacc
    33361 ccgccatgcg gaagacgttg cgacgcttga gttccgcgat gaatccgccg gtcgtcgcca
    33421 aggcccgccc ccgtttgccg gcgccgcacc ggcctcgcag atgcagtgtc ggcaatggcc
    33481 gtggcgtgtg caagccggtc ggtggtaccc gatgcgcccg ctgccagccc ggcgactgca
    33541 attcgaggcg gttgttcccg cgcatggagt cgccggcaat caagaaggcc aggaagtccc
    33601 tggcctttcg tgcggtaccg gctacggcgt gccgcgggga cggtcaggtc tgctcggcgg
    33661 gaccaccgac aacctgcgcc gtgctcgggc cgccatcctc gtccttgccc ggccgcgcgg
    33721 tcttttccac ccagcccgcg ggtggcgacg gcttgcgtcc ttccatgatc gcccggacct
    33781 gctcggcgtc gatggtttcc cattccagca gggccgcgct catcgcctcc accgcctcgc
    33841 gcctgtcctc caggatgcgg cgggcaacgc cgtactgctc gtcgaggatg cggcggatct
    33901 cggcgtccac cttctgctgg gtagcttccg aaatggtctt gttctgcagc ccgaagccgg
    33961 aatccttgtc gtccgcgtag accatggtgc cgagcgcgtc ggacatgccg tacttggtga
    34021 ccatggcgcg ggccagcttg gtcgcgcgct cgaagtcgtt ggccgcgccg gtggacatct
    34081 ggccgacgaa gatctcctcg gcgatgcggc cgccgaacag gatcgagatc tcctccagca
    34141 tcttgtcccg gtagttgctg agctggtcgt gctcgggcaa ctgccaggtc aggcccagcg
    34201 cccagccgcg cggcatgatc gtgaccttgt gcaccgggtc cgccttcggc agcgacatcg
    34261 ccaccaccgc gtggccggac tcgtggaacg cggtgttgcg gcgctcgtcc tcgcgcatga
    34321 ccatgctgcg gcgctccgga cccatgaaga tcttgtcctt ggcgtcctcg aagtcctgca
    34381 tctcgacctg gcccttgttg cggcgcgcgg cgaacagcgc ggcctcgttc accaggttgg
    34441 cgaggtcggc gccggagaag cccggggtac cgcgcgccag caggtccgcg cgcacgtcgt
    34501 cgtgcagcgg caccttgcgc atgtgcacct tcaggatctg ctcgcggccc ttg
//
LOCUS       DAORUK010000003        33357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_874_length_33357_cov_20.035884, whole genome shotgun sequence.
ACCESSION   DAORUK010000003 DAORUK010000000
VERSION     DAORUK010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 33357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33357
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_874_length_33357_cov_20.035884"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PK818_00535"
     CDS             complement(<1..625)
                     /locus_tag="PK818_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQY81252.1"
                     /translation="MRSQEQAGLVLIVEDNRNISEMVGEYLESRGFEVDYASDGLDGY
                     RLAAENDYDVVVLDLMLPRLDGIEVCKRLRGEARKSTPVLMLTARDTLDEKLTGLSSG
                     ADDYLTKPFAIQELEARLRALIRRERREVGAEVLKVADLVLDPASLRTTRGGSALQLS
                     PIGLRLLAILMRESPRVVSRQEIEREIWGNGLPDSDTLRSHLYNLRKV"
     gene            complement(739..1617)
                     /gene="mazG"
                     /locus_tag="PK818_00540"
     CDS             complement(739..1617)
                     /gene="mazG"
                     /locus_tag="PK818_00540"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HQY81253.1"
                     /translation="MSDARDIRDLLAIMAALRDPASGCPWDVVQDFASIAPYTIEEAY
                     EVADAIDRGDMADLRDELGDLLLQVVFHAQMAQEGGAFEFGDVVAAICDKMVRRHPHV
                     FAARGRASAAGGRMPGAAADEARRSNADAQLQHWEELKRAERAARGDSDVSALAGIAR
                     GLPEWQRAVKLQHRAAKVGFDWPDPAPVLEKLHEEIEEVRAEFAAVAANPGDPAARDR
                     LEDEIGDILFVCANLARHAKVDFGAALRRANHKFERRFRAMEALAEADGGLAGQSLEA
                     QDRYWVRAKQAERGEA"
     gene            complement(1614..2405)
                     /gene="cysQ"
                     /locus_tag="PK818_00545"
     CDS             complement(1614..2405)
                     /gene="cysQ"
                     /locus_tag="PK818_00545"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HQY81254.1"
                     /translation="MTVSTDRREAVIALARRAALEILAVYESPFAIEHKQDRSPLTAA
                     DLASHRCIVAGLQALTPEIPVLSEESKDLDIAARREWPTLWLVDPLDGTREFIKRNGE
                     FTVNIALIEHGVATFGVIQQPVTGALWHGAAGAGAFRREGDADVAICSRKPASAPLRI
                     AASRSHRDARTQALLDALPGSEVVGCGSSLKFCRIAEGGIDLYPRFGPTSEWDTAAGQ
                     AIVEAAGGAVLDPRGRPLRYNQRDTLLNGDFLAFGDIAVRNALPA"
     gene            complement(2402..2950)
                     /gene="nudE"
                     /locus_tag="PK818_00550"
     CDS             complement(2402..2950)
                     /gene="nudE"
                     /locus_tag="PK818_00550"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005414246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HQY81255.1"
                     /translation="MSRRLPTIHGITEHDVGPYRMERLDLEFSNGERRKYERLHGRGH
                     GAVAVVPMLDAETVLLVREYAAGMHRYELGLVKGRIDAGETALQAANRELMEEAGYGA
                     RDLLVLRSLTLAPVYMSHATDLVLARDLYPQRLPGDEPEELELVPWKLDDIGELILRE
                     EFSEGRSIAALFIAREWLRQQR"
     gene            3001..4392
                     /gene="bioA"
                     /locus_tag="PK818_00555"
     CDS             3001..4392
                     /gene="bioA"
                     /locus_tag="PK818_00555"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016851465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HQY81256.1"
                     /translation="MLHDADMIAAADGWRTRDLDVLWHPCTQMREHPGVLPLVPIERG
                     EGAWLVGRDGRRYLDAVSSWWTNLFGHAEARIAGAIARQAHTLEQVILAGFSHQPAVE
                     LAEKLLAIAPREPGRAPLAKVFYADNGSAGVEVALKMAFHWFRNRGEGKRTRFVALHN
                     GYHGETIGALAVGDIPLYRRVYAPLLAEALFAPSPDSYAAEPGETAADCAERAAEGLR
                     ELLERHSGEVCALILEPRVQCAGGMRMHDPRYLRRARDLCDAHGVLLIADEIAVGFGR
                     TGAMFACEGAGIQPDLLCLSKGLTGGFLPLAAVLATQAIYDGFLDDSRERAFLHSHSY
                     TGNPLACAAALASLAIFDTDDVLARNRATAARMAALAASIVELPHVADVRQAGMIVAF
                     ELTRDGDRHAPFEPAARIGLRAYRAALEGGVVLRPLGDTLYWMPPYCIDDEQLHLLAR
                     VTRDAILEATACA"
     gene            4383..5114
                     /locus_tag="PK818_00560"
     CDS             4383..5114
                     /locus_tag="PK818_00560"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HQY81257.1"
                     /translation="MRLTRSHVDLPLRVGEEIALPEDVAAHLLRVLRLQVGDACVLFN
                     GDGHDYDARITAVGKRDARASVLAARRVDNESPLRITLLQGIARGEKMDWILQKATEL
                     GVARILPACSERSEVRLDAQRAEKRLAHWRGILVSACEQSGRASLPEIVAPCELAQAA
                     GLRAGPGFILDPEAGESLTSLRGASLRECTLAIGPEGGWSPRDRERLLAAGYTGLRLG
                     PRVLRTETAGIAAIAALQALCGDLG"
     gene            complement(5111..5581)
                     /locus_tag="PK818_00565"
     CDS             complement(5111..5581)
                     /locus_tag="PK818_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HQY81258.1"
                     /translation="MSDSASIIRGVLIQVADARLLLPNATIAEVLSYADPEPVADAPD
                     WLLGRMRWRGWQLPLVSFSRFTGIAEERGGLGSKVIVLKALGGDTKHPFFALLTQGFP
                     RLVTVTEAGLAAVGDDAAVPEGVLARVRLNEDDALLPDLAMLETRITEALAIAA"
     gene            complement(5590..6600)
                     /locus_tag="PK818_00570"
     CDS             complement(5590..6600)
                     /locus_tag="PK818_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HQY81259.1"
                     /translation="MSVRKRVVLLTRPGSARERTEAGIIAAGAEVAAVLDPGAASEGD
                     VRNAAPDVVLVILDPAVEQALHKFDAVLGDPALDIMFEDADVAARREGWEAARWARHL
                     GAKLYGHHDVLPRAKGAPPAAAPQSRASEEESRFRDEMEALQAQVAAMPELPRGNPRA
                     AAATAGAVVIAAGIGGPDAVRQLLAGLPAGFPHPILLRQRIEGGHYDKLVRQMQRATQ
                     LPVVLAQPGDPVANTAVHVIPDGVDVHAAPAGLQFAACAGQPTFAALAAANSALLLLS
                     GADPALVDRALALAMAGGIAVGQSDENCFDPAASNALVARGGEAISLAKLPLKLLARW
                     PA"
     gene            complement(6597..>9387)
                     /locus_tag="PK818_00575"
     CDS             complement(6597..>9387)
                     /locus_tag="PK818_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017158585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY81260.1"
                     /translation="AERVAAEQAEAERLEAERLAAEQAEAERVAAEQAEAERLEAERI
                     AAEQAEAERLAAEQVEAERLEAERVAAERAEADRLEAERITAARAEAERAEAQRIAAA
                     KAEAERVEAERRLAETAAAAKAREQAALAALLASATSSEDPDEALDMSGLDAELIDIF
                     VEEGGDLLDQSDGLLAQLRDAPEDREAMVGLQRELHTLKGGARMAGIMPVGELGHAME
                     TLLEAVVAQRAELGRDGVPLLERGFDRLHGMITRVAARRAVGMPVGLIDEFNARAEAP
                     VAVAEAADDGLHFVPAPAAKIELKPLSAPMVAEGFGDDEDIGVRAPQEQVRIRADLLD
                     RLVNYAGEVAIYRSRLEQQLGAFRNTAAEMEQTNLRLRDQLRRLDAETEAQIIARYQR
                     EHDTADATFDPLELDRFSTLQQLSRALAESAADLQSLHGSMDDLTRQYETLLLQQSRV
                     SSELQEGLMRTRMVPFDALVPRLRRVIRQAAGETGKQAQLKLDGAQGELDRNVLERMT
                     APLEHMLRNAVAHGLEAPGARRSSGKPEEGSIRVAVRREGSEVVLQVSDDGGGINRQA
                     VRARAIERGLIREDAVLGDSDLDVLILEPGFSTAETVSRLAGRGVGMDVVASEVRQLG
                     GTLDIASNPGHGTTFTLRLPQTLAVTQAVFVKIGDTQYAVPIASVRGVGRITREDMAK
                     PDAAYRYGGEDYALHDLGNLVGASTTAKAEGQLQMPLLLVRSGDLRAAVAIDQVIGNR
                     EIVVKSGGPQLASVPGIFGATIMGDGSVVVILDVAPLVRRQVSMPRDVEAVEQAVEQR
                     RVPLVMVVDDSVTMRKVTGRVLERHGFEVGTAKDGLDALERMADTIPDLMLLDIEMPR
                     MDGYELATAMKADPRMRDVPIIMITSRTGEKHRQRAMDLGVQRYLGKPYQEVELMRNV
                     LELLGVERTGDGSAS"
     assembly_gap    9388..9487
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9488..12182)
                     /locus_tag="PK818_00580"
     CDS             complement(<9488..12182)
                     /locus_tag="PK818_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011269581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HQY81261.1"
                     /translation="MTTALRDAIDHTALGWVKPEIDETLRQARIEIEAFAESPGDSER
                     MRACAGYLHQVLGTLRMLELHAPAMVAEEMEHLAGALQGGATGNRDEACAALMRGVVQ
                     LPDYLERLQGGHRDIPIVLLPLLNELRAARGEAALNQDALPSLLEEPSEAELEHARGS
                     LAGRNRALLDTVSAALKEDLLRVKDALDLHLRSGLADVAALQPQAEALDRIGETLGMI
                     GLESARSLIQEQGKIVHGIASGERPANEADLLDVAGALIYIDHSLDDQVARLGQGDDD
                     AGDNLLAQESRKVIAVLAREAVANFAEVRNAFVAFVETDWDHAALDAVPRLLGEVGGA
                     MRVLQLPEPAGYLEALRRYSHAELIGKLQVPDGRQLDTLADALAGMEYYLESLREPHG
                     QRQDLLEKTRGSLELLGYWPLRDADAIDEAVAEAEAVEARVEAAATAAAVEAAKDAAP
                     VAPDVVAQQVGTPALSAGFDSAGDEIDDEIREIFLEEFEEEIGNLDELLTPWRRTPDD
                     PELLRPIRRVFHTLKGSGRLVGAKTLGEFSWHVENMLNRVLDGSRPPSQAVVAFIGQT
                     RDVLPHLHAALRGEAAVSVDLAAMEGVADRLAAGEDVLLGAVVAAATADTSAAAVEAE
                     AETPLVEPAAAETVAVQVDPVLLDILGAEVGGHLETVDAWLAQARAGDSTVNDGLLRA
                     IHTLNGAFAMTEVPSVTAFTSPAEGYVKRLLSAGEAADAGGVAVIGEVADAVRASTHA
                     LQATPAQVPVYAALVASVVALRDALPESSLPFISPAEAEREAAHEAERLEAERVAREE
                     AERLQAERVEAERRVAEQAEAERLAAEEAARLEAERLAAEEAEAARVAAEHAEADRLI
                     AEAAEAARLDEARTEAERIEAAQLAAEQAEAERLEAERLAAE"
     gene            complement(12260..14275)
                     /locus_tag="PK818_00585"
     CDS             complement(12260..14275)
                     /locus_tag="PK818_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQY81262.1"
                     /translation="MSTMMDSVAGKGQNLSTRAWAAILILSLVVFGANTLYATTKAAR
                     VGGASTAASNLQVNSQRLAVQAQDAVSGNADSYKAFKSTKEQIDGDVKSLNDNFGDAT
                     GVSGPIKAVTDTWTPLAKGAEQLLAAEPQVLALAGQADSVTAKMPQLQAQLNEVVRGL
                     ASGGAGSGQVFTALQQVVTSAAIARRIAELRAGGSGAQVAADGLARDAAVFDRSMNGL
                     RNGGGDIARVTAAGAVAPLNQAFTLWQGMKKDLDALNASSRNLLGAQASATALASGSN
                     KLLSESQRLLASVTSFGSLKDTSIVGGVWVSILSGLGILLSLVGLYFSTRSAERKRYQ
                     TTKELNDRNQEAIMRLLDEMGSLAEGDLTVKATVTEDMTGAIADSINFAVEQLRTLVT
                     TINDTSVQVASSAQETQATAMHLAEAAEHQAQEINSATDRINEIAASINQVSRNSADS
                     AEVAQRSVQIATNGAGVVRQTIAGMDSIRDQIQETSKRIKRLGESSQEIGSIVELIND
                     ISEQTNILALNAAIQAASAGEAGRGFAVVADEVQRLAERSSSATKRIESLVQTIQADT
                     NEAVSSMEQTTSEVVAGARLAEDAGTALGEIEKVSADLSGLIQGISTAAAQQSSAATG
                     ITQTMNTIQSITAQTSQGASQTAESVGILAQLAADLRRSVADFKLPA"
     gene            complement(14322..14867)
                     /locus_tag="PK818_00590"
     CDS             complement(14322..14867)
                     /locus_tag="PK818_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014508568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HQY81263.1"
                     /translation="MRARDKRSPYLVLAEYEQRSLAHVAGLPEQLDAPGLWRGVAYRI
                     GSHRLASGFDEVVEILSMPAVTPVPGSQPWMLGVANVRGTLLPVVDLKQFLEGERTVL
                     HEGQRVLVVRQPGGDVAVTIEEMYGQRSFVDAQGIDAAAVTEGRYAYFIDRAYRINDQ
                     DWGVFSLERLARTPEFRQAAA"
     gene            complement(15165..15530)
                     /locus_tag="PK818_00595"
     CDS             complement(15165..15530)
                     /locus_tag="PK818_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY81264.1"
                     /translation="MARILLIEDSPTEAAVMTQLLERNGHQVTTSGSAEDGIAAARRD
                     KPDLVLMDVVLPGMNGFQATRALSRDADTSHIPVLIVSTKNLDTDKMWGLRQGAKDYI
                     VKPPRETDLIERINALVGH"
     gene            complement(15549..15947)
                     /gene="pilG"
                     /locus_tag="PK818_00600"
     CDS             complement(15549..15947)
                     /gene="pilG"
                     /locus_tag="PK818_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005913706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HQY81265.1"
                     /translation="MTQDAQDVSLKGLRVMVIDDSKTIRRTAETLLVREGCDVVTATD
                     GFEALAKIADHDPQIIFVDIMMPRLDGYQTCALIKNNQTFKSVPVIMLSSKDGLFDKA
                     RGRIVGSEQYLTKPFTREELLGAIRTYVNA"
     gene            16108..17070
                     /gene="gshB"
                     /locus_tag="PK818_00605"
     CDS             16108..17070
                     /gene="gshB"
                     /locus_tag="PK818_00605"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HQY81266.1"
                     /translation="MPAVAPLDVVVVMDPIESIKPAKDSSFAMLLEAQRRGHRLHYVR
                     PGGLSVSGARAVATVAPLGVRDQANEHFELGAWSRLEFGPGQVVLMRKDPPVDADYLH
                     DTQILGMARLQGALVVNDPQGLRDFNEKLAALLFPQCCPPTLVSRDATALKAFVGEHG
                     EAVLKPLDGMGGRSIFRARAGEANLNVILETLTQGGAHLAMAQRYLPEIVAGDKRILL
                     VDGEPVDYCLARIPQGDEFRGNLAAGGRGEGRPLTERDRWIAAQVGPEMRRRGMLFVG
                     LDVIGDYLTEVNVTSPTCIRELDAQFGLDIAGTLFERIEAKLAS"
     gene            17154..17969
                     /locus_tag="PK818_00610"
     CDS             17154..17969
                     /locus_tag="PK818_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQY81267.1"
                     /translation="MVLSVLVHAMLILGIGFAVEDAAPMMPTLDVILSQTRTALTPKQ
                     ADFLAQADNQGGGEHDKSSRPTAPQAGPLPRPEDGLAPRPLRAQTPAPSPPPETRVVT
                     SRNARQVLPTPQPRQEIEASVLPPGSEKIDRDIEMARLAAEIQLRSQQYAKRPKRKFV
                     SASTKEYAWAQYLRGWVDRVERVGNLNYPDEARRRRIGGLVVISVAVRRDGSVESTRI
                     IQSSHVPMLDSAALRIVKLSEPFAPLPKTQDDPDILHVTRTWQFMPGGELIDQ"
     gene            complement(17987..18679)
                     /gene="tsaB"
                     /locus_tag="PK818_00615"
     CDS             complement(17987..18679)
                     /gene="tsaB"
                     /locus_tag="PK818_00615"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HQY81268.1"
                     /translation="MRLLAIETATEACSVAILVDGDVRERFEIAPRRHAELALPWAEQ
                     LLADAGVAKPQLDAIAVGRGPGAFTGVRLAIALAQGIALALDRPLLPVSTLAALAMRA
                     EGGRIVAAIDARMGEVYLAAFARAGDDVVALGEEVVVKPDAAILPAGDCWHGVGTGFA
                     AGDNALQARMQGRLATVDPQALPHAADVARLGALLFARGGAVAPERIEPAYLRNNVAL
                     TIAEQQALRASR"
     gene            complement(18683..20689)
                     /locus_tag="PK818_00620"
     CDS             complement(18683..20689)
                     /locus_tag="PK818_00620"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HQY81269.1"
                     /translation="MSTSRLGAGARDALSEGAALAQGIPGFVARPAQQRLAMAVAEAF
                     EQRGVLLAEAGTGTGKTFAYLVPALLSGMKTIISTGTRALQDQLYFRDLPRVRDALGI
                     GLKTALLKGRSNYLCLYRMEQAKGDPQLSKGGFSSREQVAQFQRVVAWSGRTKFGDMA
                     ELDALPEDSPLLPQVTSTADNCLGSECPSWSDCFVVQARQRAQTADLVVVNHHLLLAD
                     LALKQEGFGEILPGAQAFVVDEAHQLPELAAQFFGEGLGARPLVELARDAIAECKDVP
                     GALGSLQEPARMLEQAARALRAGMDHLPQRGTRWRALEGEGVEAGFQALEHALQALQQ
                     ALQPLREAAPGFDACHLRANDQLGRLRRWLGEAGSGPRPDAGMPRSADVPAASGPDPV
                     SVHWYELTPRGFRLQRTPLDVSAPLRAHRDASHAAWVFTSATLAVAGGFSHIAQRLGI
                     DTAVELIEPSPFDWDRQALCYLPPRLPEPAARDFGAALVDALLPVLHASAGRAFLLFA
                     SHRALREAAELLRDGPWPLFVQGSEPRHVLLQRFRESGNGVLLGTASFREGVDVAGDA
                     LSVVVIDKLPFAAPDDPVFEARLDAIRRSGGNPFRDEQLPQAVIALKQGAGRLIRTER
                     DRGVLVLCDPRLTQKSYGRVFLESLPPIPRTRELADVQAFFADN"
     gene            complement(20706..23177)
                     /gene="mrcB"
                     /locus_tag="PK818_00625"
     CDS             complement(20706..23177)
                     /gene="mrcB"
                     /locus_tag="PK818_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HQY81270.1"
                     /translation="MPRIEDEDDDLHDEDREGGEGGIDWRSRLLTWGLAAAALLLGFL
                     VPYMLYLNHQVGERFGKLRWQVPTRVYARPLTLRDGLAMDAQTLKTELDAASYHGGDG
                     KRAGTYARNGSRWHIASRGYDDVDGRIAPARLEVTLSGGEVIAIRDLGSRKALRSARL
                     DPARIATLYGQQQEERRLVRIEEVPELLVTGLQAVEDRDFAHHLGVDFSGMLRALFVN
                     VKSGGDTRQGASTLTQQLARSGLLGIGKEQTYTRKFKEILFALLLEARYDKRTILEAY
                     FNQVDLGQRGAQAIRGVAAASEFWFGRDLRDLSTEQVALLIGLVKGPSWYNPRRNPAR
                     ATERRNFVLGKMHESGLIDDAEFKRATTAPLGITENAGNVAANRFPSYVDLVRKQLAR
                     DYPADSISGAGLSVMTGMSPSAQGYAEGAVARTLKSVGGKGRPPLQAGVVMTDVHNGD
                     VVAVVGSRNFGEQGFNRAVDARRPVGSLLKPFVYLLALAQPDKWSLASTISDAPVTVS
                     LGRGRDWKPGNSDNRSHGTVRLVDALAQSYNQATVRLGMDVQPQRLADLIRTLAGITS
                     EGQPSLILGAVDQSPYAMAQLYQFLAADGEIQPLHAVRGVLDPQGRTIKRYDSAPPPA
                     QKGDAVAARLVGTALQYAVTSGTGRPLLRDGLGRLAPAGKTGTSNDSRDSWFAGYTGD
                     HLAVVWVGNDQNQPTGLYGATGGMRVWSDIFARLPSATLRVPGEGLDWQWVEGGHSTD
                     PGCPGARRFAFVAGYAPTYRQCVYAPPPAFDEFGNPLPPDDGSAPLERAGQAIRDFLG
                     LGDGRDPATQAPPEPAPPADPVPQP"
     gene            23319..25409
                     /locus_tag="PK818_00630"
     CDS             23319..25409
                     /locus_tag="PK818_00630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY81271.1"
                     /translation="MPIPAEARFWIARALGLWRRGWASLRTRGLAASWRRLQLHFRRP
                     AVPALPLYAPDALPFAPFAIATSDAPRASIVVPVHGAFAHTLACLRALAAHPPRAAFE
                     LIAVDDASPDDSLARLAQVGGIRVHARARNGGFIAACNEGAALARGDYLVFLNNDTVP
                     QPGWLDALLGTFDQHPGTGLAGAKLVYPDGRLQEAGGVVFADGSGWNYGRNDSPDDCR
                     YACVRAADYCSGAAIAIPRGLFERLGGFDQRYAPAYYEDTDLAFAVRAAGLRVLYQPA
                     AVVVHDEGATAGTDLAQGPKAAQVRNQSVFAHKWQDALRTQLPAGSIPTPALLHRGQP
                     QVLIVDALTPQPDRDSGSLRLVNLMRLLIGEGAHVVFLPANRSADGAYTTALQQLGVE
                     CWHAPFATGIPAWLREHGPRFDVAMVSRHYVASELLPLLRKHAPRARVLFDTVDLHYL
                     RERRAAGLSGDAAALRAAQRTREAELALVAQADATLVVSEAERALLAEDAPGAQVRVL
                     SNLHEPGADGLAWSQRRDLLFVGGFRHPPNVDAVLWFVREVFPRIRAARGDVRFHCIG
                     GDVPAEVQALAASDGVQIHGHVPDLQPWLAGCLAAVAPMRYGAGVKGKVNQAMAHGLP
                     VVATTPAAEGMHLVDGRDVLVADAAEAFATAVLRLHQDEALWTRLAANGRASVARHFS
                     MDAARGVVRELFLH"
     gene            complement(25507..27666)
                     /locus_tag="PK818_00635"
     CDS             complement(25507..27666)
                     /locus_tag="PK818_00635"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019336379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HQY81272.1"
                     /translation="MPAPVAAAKMHYADALSRLPAGALPEPLAALWSAQAEAGAPMRD
                     RADVLADTLRILASLNADADVVAAALLDALVDVERSAAGQGVAIPERLQALLEGQRAA
                     SRVWALHRSHGGGRNPEGMRRLLLALVRDLRVVPIVLARQLSRMRAAWRLPVEEQRAL
                     AQLTRDIHAPLANRLGIWQLKWELEDLAFRSLETDTYLRIARLLDDNRSGRERYIEQA
                     KAQLQEALAAQGIANADIAGRPKHIFSIWKKMRRKDAPIGELYDLRALRVLVEDVADC
                     YAVLGLVHALWLPVPSEFDDYIARPKSNGYRSLHTAVVGPGGKTLEVQIRTREMHAQA
                     ELGVAAHWRYKEGGAAGGAAMDKRIAWMRKLLEQAAEAARDGEASPLDAMDSELVEDR
                     VYALTPKGEVVDLQQGATVLDFAYQVHTMVGHRCRGAKVDGRIVPLNTQLRSGERVEI
                     LTGKTAEPRRDWLQASSGFLASNRSREKVRAWFNKLDRARNLQAGRELLDRDLRRLGL
                     LHADLQPVLGRFNVATVDELLVLVALGDVGPNQVGRALLELEKPAEEAPPLPSTAPPK
                     ATTPAAGTVTVQGVDNLLAQVARCCQPLPGEPIAGYLTRARGVSVHRPDCASFLRLAG
                     RQPARVLPVEWGARSGGHEVAIRIEAVDRKWLLKDITTLLAQQGVHVLGLHADQLRDG
                     DPRVRLNVRVRVADYGQLGLLLGRLEAMAGVESARRG"
     gene            complement(27721..31719)
                     /gene="hrpA"
                     /locus_tag="PK818_00640"
     CDS             complement(27721..31719)
                     /gene="hrpA"
                     /locus_tag="PK818_00640"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HQY81273.1"
                     /translation="MQATENNGKTASRQARRAIDGALSKDRGRLLALWSKWNAKPDDP
                     ALREGFAAKLQASVAERARRAAALPAASVDTTLPIAAHADEIVALIRAHQVVVVAGET
                     GSGKTTQLPKLCLLAGRGAAGMVGCTQPRRIAARSVARRVAEELQVPLGGAVGFQVRF
                     NDAVGEQTAIKFMTDGILLAEIQTDRWLSKYDTLIIDEAHERSLNIDFLLGYLKQLLP
                     KRPDLKLIVTSATIDTARFAEHFGGAPVVGVEGRGFPVEMRYRPLGEPARAGGQLVRR
                     TGERPATDGRAGRSEGGEVSPRMAAESLEGAGERTIVDGIVAACDEIMRDRGIGDTLI
                     FLPGEREIRDAHQALEKRKYRHTEVLPLYARLSAKDQDRVFQPGPQRRIVLATNVAET
                     SLTVPRIHYVIDPGVARVKRYSPRQKLDRLHIEDVSQASANQRAGRCGRIAPGTCFRL
                     YSEADYASRPEFTDPEIRRAALAGVILRMLSLGLGSIEDFPFLEPPDSRAVADGWQQL
                     NELGAVDANRKLTAIGKAMAKLPVDVKLARMLVAAQVHGVLREMLVIASFLGIQDPRE
                     RPADARGAADAAHAQFADAKSEFIGILKLWQAYREAHEDMTQSQLRKWADKHFLGFLR
                     LREWWELHRQLKLQCDELGWQQNDEPGGFAELHRALIAGLPTQIGNRGDKGLYDGPRG
                     RKFQLFPGSRLASKPPPWVLSANLLDTEKVWAITNAGIEPDWVIAELPHLLARRHHDP
                     HWSRAQGRVLGSEQISLFGLVLAPKRPIHYGALFPEESRGIFVREALLAGEINTRSGF
                     LARNLRTLALAQQEEAKQRRVGLVVDEDWQARWYLDRLPAHVHNAQALDAWFGKLSKD
                     EKAALEWTRDDLLVADQTDAARFPAYLALGDVRLAVQYRFEPGAVDDGMTVALPLHLL
                     NALDAARLSWLAPGFVEDKAVALIRSLPKALRRNFVPAPDFARAFAEAHATVEPDSIE
                     GALARFLKKLSGVEVSALDFDAAAIAPYLRANLRLLDGKAVLAESRDLAELRARFGER
                     AAQAFARHAAQGMAQAGMTGFPAAPVPVSVPGAGGVPAYPALQDDGDSVSLALHAQRE
                     VAERLHPQGVRRLLAIALADKLKQARKQLPVQPKISLLYAAIESVDPRRDGLKDADRL
                     RSDLVDGAFAALAADGLLAIRDAEAFAQRRDALAKALFGEAMQRLRQADTILGLVADV
                     RAKLESKLMGWARANLDDMQAQLAALAAPGFLREVPAEVLAEYPRWLKALVLRAERAQ
                     RDPTRDQARMLELKPFADALAAADPADADAQALRRELQELRVQLFAQELGVKGGVSPK
                     RLAARLQRLQRP"
     gene            31802..32368
                     /locus_tag="PK818_00645"
     CDS             31802..32368
                     /locus_tag="PK818_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010364158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HQY81274.1"
                     /translation="MAKKKSAPKSTAKPAPKADKPLPTAHADGAPGIDIGISTGDRRK
                     VADGLSHFLADAYTLYLKTHNFHWNITGPMFNSLHVMFEGQYTEQWTALDEIAERIRA
                     LGFKAPGSYAEFVRLSSIPEEPGLGDSADWREMVRQLVVGNEAVCRTARKVLATADKA
                     GDDPTVDLMTQRLNIHEKNAWMLRSLLQ"
     gene            32390..>33357
                     /locus_tag="PK818_00650"
     CDS             32390..>33357
                     /locus_tag="PK818_00650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HQY81275.1"
                     /translation="MGAPALEQLRHVFGYDSFRGEQADIVGHLVAGGDALVLMPTGGG
                     KSLCYQLPALLRAGTAIVVSPLIALMQDQVEALRQLGVRAAFLNSSLDAGAAAQVERE
                     LLDSQLDLLYVAPERLLTPRFLSLLERTPVALFAIDEAHCVSQWGHDFRPEYRQLTLL
                     HERWPDVPRIALTATADAPTRREIVERLALEDARQFVSSFDRPNIRYTVVAKDDSRRQ
                     LLEFLDDHRGDSGIVYCLSRRKVDATAELLAGRGVRALPYHAGLDAATRAEHQRRFLR
                     EDGIVMVATIAFGMGIDKPDVRFVAHLDLPKSLEGYYQETGRAGRDG"
BASE COUNT         4643 a        12006 c        11474 g         5134 t
ORIGIN      
        1 tgaccttgcg caggttgtag aggtggctgc gcagggtgtc cgaatccggc aggccgttgc
       61 cccagatctc gcgctcgatt tcctggcggc tgaccacccg cggcgattcg cgcatcagga
      121 tcgccagcag ccgcagcccg atcggcgaaa gctgcaacgc gctgccgccg cgggtggtcc
      181 gcagcgacgc cgggtccagc accaggtcgg cgaccttcag cacctccgcg ccaacctcac
      241 ggcgttcgcg gcggatcagc gcacgcagcc gcgcctccag ttcctggatc gcgaacggct
      301 tggtcaggta gtcgtcggcg ccggacgaca gcccggtcag cttttcatcg agggtgtcgc
      361 gcgcggtcag catcagcacc ggcgtggact tgcgcgcctc gccgcgcagc cgcttgcaga
      421 cttcgatgcc atccagccgc ggcagcatca ggtccagcac caccacgtcg tagtcgttct
      481 ccgccgccaa ccggtagcca tcgaggccat cggaggcgta atcgacctcg aagccacggc
      541 tctcgaggta ctcgccgacc atctcggaaa tgttccggtt gtcctcgacg atgaggacca
      601 atccggcctg ttcctggctt cgcatcggtg accgctccgg attttcaact tcgtggaaga
      661 tgccggcagc gcggtggaag caacgtgaag ggcagccgcc ggcgggcgcc tgcggccttc
      721 cggcgatacc tgcgcgggtc aggcctcgcc gcgctccgcc tgcttggcgc gcacccaata
      781 gcggtcctgc gcctccagcg attgcccggc caggccgcca tcggcttcgg caagcgcttc
      841 catcgcccgg aaccggcgct cgaacttgtg gttggcacgc cgcagcgccg cgccgaaatc
      901 caccttcgca tgccgcgcca ggttggcgca gacgaacagg atgtcgccga tctcgtcctc
      961 cagccggtcg cgcgccgccg gatcgccggg gttggccgcc accgccgcga actccgcgcg
     1021 gacttcctcg atttcctcgt gcagtttctc cagcaccggc gccggatccg gccagtcgaa
     1081 gccgaccttg gccgccctgt gctgcagctt caccgcgcgc tgccattccg gcagcccgcg
     1141 cgcgatcccg gccagtgccg acacatcgct gtcgccgcgc gccgcgcgtt ccgcgcgctt
     1201 gagctcttcc cagtgctgca actgggcatc ggcgttgctg cggcgggcct cgtcggcggc
     1261 tgctcccggc atcctgcctc ccgccgcact tgcacgtccc cgtgcggcga acacgtgcgg
     1321 atgccggcgc accatcttgt cgcagatcgc cgccaccacg tcgccgaatt cgaaggcacc
     1381 gccttcctgc gccatctgcg catggaacac gacctgcagc agcaggtcgc cgagttcgtc
     1441 ccgcaggtcg gccatgtcgc cgcggtcgat cgcatcggcg acctcgtagg cttcctcgat
     1501 ggtgtaaggc gcgatgctgg cgaagtcctg cacgacatcc cacgggcagc ccgatgcggg
     1561 atcgcgcaac gcggccatga tcgccagcag gtcgcggatg tcgcgcgcgt cgctcatgcc
     1621 ggcaatgcgt tgcgcacggc gatatcgccg aaggcgagga aatcgccgtt gagcaaggtg
     1681 tcgcgctggt tgtagcgcaa cgggcgtccg cgcggatcca gcaccgcgcc gcccgccgcc
     1741 tccacgatcg cctggccggc ggcggtgtcc cattcgctgg tcgggccgaa gcgggggtac
     1801 aggtcgatgc caccctcggc gatcctgcag aatttcagcg acgagccgca gcccaccact
     1861 tcgctgccgg gcagcgcgtc gagcagcgcc tgcgtgcgtg cgtcgcgatg cgagcggctg
     1921 gcggcgatgc gcagcggcgc cgatgcgggc ttgcgcgagc agatggcgac atctgcgtcg
     1981 ccctcgcgcc ggaatgcgcc tgcacccgct gccccgtgcc acaacgcgcc ggtgaccggt
     2041 tgctggatca cgccgaaggt cgccacgcca tgctcgatca gcgcgatgtt gacggtgaac
     2101 tcgccgttgc gcttgatgaa ctcgcgggtg ccgtccagcg gatccaccag ccacagcgtc
     2161 ggccattcgc ggcgcgcggc gatgtcgagg tccttggatt cctcggacaa caccgggatc
     2221 tccggcgtca gcgcctgcag gccggccacg atgcagcggt gcgaggcaag gtcggccgcg
     2281 gtcagcggcg aacggtcctg cttgtgctcg atggcgaacg ggctctcgta gaccgccagg
     2341 atttcgagcg ccgccctgcg tgcgagtgcg atgaccgcct cgcgccggtc cgtcgagacg
     2401 gtcatcgctg ctgcctcagc cattcgcggg cgatgaacag cgcggcgatg ctgcggcctt
     2461 cggagaattc ctcgcgcagg atcagctcgc cgatgtcgtc cagcttccac ggcaccagtt
     2521 ccagttcctc cggctcgtcg ccgggcaggc gctgcggata caggtcgcgg gccagcacca
     2581 ggtcggtggc gtggctcatg tacaccggcg ccagggtcag gctgcgcagc accagcaggt
     2641 cgcgcgcgcc gtacccggcc tcctccatca gttcgcggtt tgccgcctgc aaggcggtct
     2701 cgccggcgtc gatgcggccc ttgaccagcc cgagttcgta gcggtgcatg ccggctgcgt
     2761 attcgcgcac cagcagcacg gtttcggcat ccagcatcgg caccaccgcc accgcgccat
     2821 gcccgcgccc gtgcaggcgc tcgtacttgc ggcgctcgcc gttgctgaac tccaggtcca
     2881 gccgctccat ccggtacggg ccgacgtcgt gctcggtgat gccgtggatg gtgggcaggc
     2941 gccggctcat ggcgcgcagc ctgccttgcc ggggttcggg gcgataatgg cgggatgggg
     3001 atgcttcacg atgccgacat gatagctgcc gccgacgggt ggcggacacg cgacctggac
     3061 gtgctctggc acccgtgcac gcagatgcgc gagcacccgg gcgtgctgcc gctggtgccg
     3121 atcgagcgcg gcgagggtgc ctggttggta ggccgcgacg ggcgccgcta cctggatgcg
     3181 gtcagcagct ggtggaccaa cctgttcggc cacgccgagg cgcgcatcgc cggggccatc
     3241 gcccgccagg cgcacaccct ggagcaggtg atcctggccg ggttctcgca ccagccggcg
     3301 gtggagctgg cggagaagct gctggccatc gccccccgcg agcccggccg cgcgccgctg
     3361 gccaaggtgt tctatgccga caacgggtcg gccggggtcg aagtcgcgct gaagatggcg
     3421 ttccactggt tccgcaaccg cggcgaaggc aagcgcacca ggttcgtggc gctgcacaac
     3481 ggttaccacg gcgaaaccat cggcgcgctt gcggtcggcg atatcccgct gtatcgccgc
     3541 gtgtacgcgc cgttgctggc cgaagccttg ttcgcgccct ctcccgacag ctacgccgcg
     3601 gaaccggggg aaaccgcggc cgattgcgcc gagcgcgccg ccgaaggctt gcgcgagttg
     3661 ctggaacgcc attccggcga ggtctgcgcg ttgatcctgg agccgcgcgt gcagtgcgcc
     3721 ggtggcatgc gcatgcacga tccgcgctac ctgcgccgcg cgcgcgacct gtgcgacgcg
     3781 cacggcgtgc tgctgatcgc ggacgagatc gcggtcggct tcggccgcac cggcgcgatg
     3841 ttcgcgtgcg agggcgccgg catccagccg gacctgctgt gcctgtcgaa gggcctgacc
     3901 ggcggcttcc tgccactggc cgcggtgctg gccacccagg cgatctacga cggtttcctc
     3961 gacgactcgc gcgaacgcgc cttcctgcat tcgcacagct acaccggcaa cccgctggcc
     4021 tgcgccgcgg cgctggcctc gctggcgatc ttcgataccg acgacgtgct ggcgcgcaac
     4081 cgcgccaccg ccgcgcggat ggcggcactg gccgcgtcga tcgtggaatt gccgcacgtg
     4141 gcggacgtgc gccaggccgg gatgatcgtg gccttcgaac tcacccgcga cggcgaccgc
     4201 cacgcgccgt tcgagccggc ggcgcgcatc ggcctgcgcg cctatcgggc ggcgctggag
     4261 ggtggcgtgg tgctgcgccc gctcggcgat acgctttact ggatgccgcc ctactgcatc
     4321 gacgacgaac aactgcatct cctcgcacgc gtcacccgcg acgccatcct cgaggccacc
     4381 gcatgcgcct gacccgcagc catgtcgacc tgccgttgcg ggtcggcgaa gagatcgccc
     4441 tgcccgagga tgtcgccgcg cacctgctgc gggtgctgcg gctgcaggtc ggcgacgcct
     4501 gcgtgctgtt caacggcgac ggccacgact acgacgcgcg catcaccgcg gtcggcaagc
     4561 gcgatgcgcg ggcatcggta ctggccgcac gccgcgtgga caacgagtcg ccgttgcgga
     4621 tcaccctgct gcagggcatc gcccgcggcg agaagatgga ctggatcctg cagaaggcga
     4681 ccgaactcgg ggtggcgcgg atcctgcccg cctgcagcga acgcagcgaa gtgaggctgg
     4741 acgcgcagcg cgccgaaaag cgcctggcgc actggcgcgg gatcctggtg tccgcctgcg
     4801 agcaaagcgg ccgtgcgagc ctgccggaga tcgtcgcgcc atgcgagctg gcgcaggcgg
     4861 ctgggttgcg cgccggaccc ggcttcatcc tcgaccccga ggcaggcgag tcgctcacct
     4921 cgctgcgcgg ggccagcctg cgcgagtgca cgctggcgat cggccccgag ggcggctggt
     4981 cgccgcgcga ccgcgaacgc ctgcttgcgg ccggctacac cgggctgcga ctggggccgc
     5041 gcgtgctgcg caccgaaacc gccgggatcg ccgcgatcgc ggcgctgcag gcgttgtgcg
     5101 gggacctggg ctaggcggcg atcgccagcg cttcggtgat ccgcgtttcc agcatcgcca
     5161 ggtccggcag cagggcgtcg tcctcgttca ggcggacccg ggccagcaca ccttccggaa
     5221 ccgcagcatc gtcgcccacc gcggccaggc cggcttcggt caccgtcacc aggcgcggga
     5281 agccctgggt caacagggcg aagaacgggt gcttggtatc gccgcccagc gccttgagca
     5341 cgatcacctt gctgccaagg ccaccgcgct cctcggcaat cccggtgaag cgcgagaacg
     5401 acaccagcgg cagctgccag ccgcgccagc gcatgcggcc cagcagccag tcgggcgcat
     5461 cggcgaccgg ctccggatcg gcgtaggaca gcacttcggc gatggtcgcg ttcggcagca
     5521 gcaggcgcgc atcggccacc tggatgagca cgcctcggat gatggatgcg gagtcggaca
     5581 tggggaacct caggcgggcc agcgggcaag cagtttgagc ggcagcttgg cgaggctgat
     5641 ggcctcgccg ccgcgtgcga ccagcgcatt gctggcggcg ggatcgaaac agttttcgtc
     5701 ggactggcca accgcgatgc cgccggccat ggccagcgcc agcgcgcggt cgaccagtgc
     5761 cgggtcggcg ccgctgagca gcagcagggc gctgttggcc gcggcgagcg cggcgaacgt
     5821 aggttgcccc gcgcaggccg cgaactgcag cccggccggc gcggcgtgga catcaacgcc
     5881 atccgggatc acgtgaacgg ccgtattcgc caccgggtcg cccggctgcg ccagcacgac
     5941 cggcagctgg gtggcccgct gcatctgccg caccagcttg tcgtaatggc caccctcgat
     6001 gcgctggcgc aacaggatcg gatgcgggaa tccggccggc agtcctgcaa gcaactggcg
     6061 caccgcatcc ggaccaccaa tcccggccgc gatcacgact gcgcccgcgg ttgccgctgc
     6121 ggcgcgtgga ttgccacgcg gcaactccgg catcgccgcg acctgcgcct gcagggcttc
     6181 catttcgtcg cggaagcgcg actcctcctc gctggcgcgc gactgcgggg cggctgcagg
     6241 cggcgcgccc ttggcacgcg gcagcacgtc gtgatggccg tacagcttgg caccgaggtg
     6301 gcgcgcccag cgcgccgcct cccagccttc gcgcctggcg gcgacgtcgg cgtcctcgaa
     6361 catgatgtcc agcgccgggt cgccgaggac cgcgtcgaac ttgtgcagcg cctgctcgac
     6421 ggcaggatcg aggatcacca gcaccacgtc cggcgcggcg ttgcgcacgt cgccctcgct
     6481 tgcggccccg gggtcgagca ccgccgcgac ctccgcgccg gcggcgatga tcccggcctc
     6541 ggtgcgctcg cgtgcgctgc ccgggcgcgt cagcaacacg acgcgcttgc gaacgctcac
     6601 gacgccgaac cgtcgcccgt gcgttcgacc ccgagcaact cgagcacgtt gcgcatgagt
     6661 tcgacttcct ggtacggctt gccgaggtag cgctgcacgc cgaggtccat cgcgcgctgg
     6721 cggtgctttt cgccggtacg cgaggtgatc atgatgatcg gcacgtcgcg catgcgtggg
     6781 tccgccttca tcgcggtcgc cagctcgtag ccgtccatgc gcggcatttc gatgtcgagc
     6841 agcatcaggt ctgggatcgt gtccgccatc cgttccagcg cgtcgaggcc gtccttcgcg
     6901 gtacccacct cgaatccgtg ccgttccagc acgcggccgg tgaccttgcg catggtgacc
     6961 gagtcgtcga ccaccatgac cagcggcacc cggcgttgct cgaccgcctg ttccaccgct
     7021 tccacgtcgc gcggcatcga cacctggcgg cgcaccagcg gcgcgacgtc gaggatcacc
     7081 accaccgagc catcgcccat gatggtggcg ccgaagatgc ccggcaccga cgccagctgc
     7141 ggaccgcccg acttcaccac gatctcgcgg ttgccgatca cctggtcgat cgccaccgcc
     7201 gcgcgcaggt cgccggaacg gaccagcagc aacggcatct gcaactggcc ttccgccttc
     7261 gcggtggtgc tggcgccgac gaggttgccg aggtcgtgca acgcgtagtc ctcgccaccg
     7321 tagcggtagg ccgcgtccgg cttggccatg tcctcgcggg taatccggcc gacgccgcgg
     7381 accgatgcga tcgggaccgc gtactgggtg tcgccgatct tgacgaacac cgcctgggtc
     7441 accgccagcg tctgcggcag gcgcagggtg aaggtggtgc catggcccgg gttcgaggcg
     7501 atgtcgaggg tgccgccgag ctggcgcact tcgctggcga ccacgtccat gccgacgccg
     7561 cggccggcca ggcggctgac cgtttccgcg gtcgagaagc cgggctccag gatcagcacg
     7621 tcgaggtcgg aatcgccgag caccgcatcc tcgcggatca gtccgcgttc gatcgcacgc
     7681 gcgcggaccg cctggcgatt gatgccgcca ccgtcgtcgc tgacctgcag caccacttcc
     7741 gaaccttcac ggcggaccgc gacccggatg ctgccttcct ccggcttgcc cgagctgcgg
     7801 cgcgcgcctg gcgcttccag cccgtgcgcg actgcgttgc gcagcatgtg ctccagcggc
     7861 gcggtcatcc gctccagcac gttgcggtcg agttcgccct gggcaccgtc cagcttgagc
     7921 tgggcctgct tcccggtctc gccggcggcc tggcggatga cccggcgcag gcgcggcacc
     7981 agcgcatcga acggcaccat gcgcgtgcgc atgaggcctt cctgcagctc cgagctcacc
     8041 cgcgactgct gcagcagcag ggtttcgtac tgccgcgtga ggtcgtccat cgacccatgc
     8101 aggctctgca agtccgccgc cgattcggcg agcgcgcgcg acagctgctg cagggtgctg
     8161 aagcggtcga gctccagcgg gtcgaaggtc gcgtccgcgg tgtcgtgctc gcgctggtag
     8221 cgggcgatga tctgcgcttc ggtttccgcg tcgaggcggc gcaactggtc gcgcaggcgc
     8281 aggttggtct gctccatttc cgcggcggtg ttgcggaacg cacccagctg ctgttccagc
     8341 cgcgagcggt agatcgccac ctcgcccgcg tagttcacca ggcggtcgag caggtcggcg
     8401 cggatgcgca cctgctcctg cggcgcgcgc acgccgatgt cctcgtcgtc gccgaagcct
     8461 tcggccacca tcggcgccga cagcggcttg agctcgatct tcgcagccgg cgcgggcacg
     8521 aagtgcaggc cgtcgtcggc ggcctcggcc accgcgaccg gtgcttcggc acgggcattg
     8581 aactcgtcga tcagaccaac cggcatgccg accgcgcgac gcgcggcgac gcgggtgatc
     8641 atcccgtgca ggcggtcgaa accgcgctcg agcaggggca cgccatcgcg gccgagttcg
     8701 gcacgctggg cgaccaccgc ttccagcagg gtttccatcg cgtggccgag ttcgcccacc
     8761 ggcatgatgc cggccatgcg cgcgccgccc ttgagggtgt gcagttcgcg ctgcaggcca
     8821 accatggcct cgcggtcttc cggcgcgtcg cgcagctgcg cgagcaggcc atcggactgg
     8881 tcgagcaggt cgccgccttc ctcgacgaag atgtcgatca gttccgcgtc cagcccgctc
     8941 atgtcgagcg cctcgtccgg atcctcggac gaggtggcgc tggccagcaa cgccgccaac
     9001 gcggcctgtt cgcgggcctt ggcggcagcc gccgtctccg cgagccggcg ttcggcttcg
     9061 acccgctccg cttcggcctt ggccgccgcg atcctctggg cttccgcgcg ttcggcttcc
     9121 gcgcgcgcag cggtgatccg ctctgcttcc aggcgatcgg cttccgcgcg ttcggctgca
     9181 acccgctccg cttccaggcg ctcggcttcc acctgctccg cggcaagccg ctcggcctcg
     9241 gcctgttcgg ccgcaatgcg ctccgcttcc aggcgctcgg cttccgcctg ctcggcggca
     9301 acgcgttccg cctcggcctg ctcggccgca aggcgctccg cttccaggcg ctcggcttcc
     9361 gcctgctcgg cggcaacgcg ttccgccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnngct cggccgcaag gcgctccgct tccaggcgct cggcttccgc ctgctcggcg
     9541 gcgagctgcg ccgcttcgat gcgttcggct tcggtgcgcg cctcgtccag gcgcgcggct
     9601 tccgcggctt cggcgatcag gcgatccgcc tcggcatgtt ccgcggcgac ccgtgccgct
     9661 tccgcctctt cggcggcaag gcgctctgcc tccagccgcg ccgcttcctc ggcggcgagg
     9721 cgctcggctt cggcctgctc ggcgacccgg cgttccgctt ccacgcgctc ggcttgcagg
     9781 cgctcggctt cctcgcgggc aacgcgctcg gcttcgaggc gctcggcttc gtgtgccgcc
     9841 tcgcgctcgg cctcggcggg gctgatgaac ggcaggctcg actcgggcag cgcatcgcgc
     9901 aatgcaacca ccgaggcgac cagcgccgca tacaccggca cctgcgcagg cgttgcctgc
     9961 agggcatggg tggacgcgcg caccgcgtcg gcaacctcgc cgatcacggc gacaccgccg
    10021 gcatcggccg cctcaccggc ggacagcagg cgcttgacgt agccctccgc aggcgacgtg
    10081 aacgccgtca ccgacggcac ttcggtcatc gcgaaagcgc cgttgagagt gtggattgcg
    10141 cgcagaaggc cgtcgttgac ggtgctgtcg ccggcacgcg cctgcgccag ccaggcatcg
    10201 acggtttcca ggtggccgcc gacttcggcg ccaaggatgt ccagcagcac cggatcgacc
    10261 tgcaccgcaa ccgtctcggc ggcggcaggt tcgaccagag gtgtttcggc ttcggcttcg
    10321 accgcggccg cactggtgtc cgccgtggcc gcggccacga ctgcgcccag caacacgtcc
    10381 tcgccggccg cgaggcggtc ggcaacgcct tccatcgccg ccaggtccac gctgaccgcg
    10441 gcctcgccgc gcaacgcggc atgcaggtgc ggcaacacat cgcgggtctg gccgatgaag
    10501 gcgaccaccg cctggctggg cggccgcgaa ccgtccagca cgcggttgag catgttctcg
    10561 acgtgccagc tgaactcgcc gagggtcttg gcaccgacca gccggccact gcccttcagg
    10621 gtgtggaaca cgcggcggat cgggcgcagc aattcggggt cgtcgggcgt gcgccgccac
    10681 ggcgtgagca attcatcgag gttgccgatt tcttcctcga attcctccag gaagatctcg
    10741 cggatttcgt cgtcgatctc gtcgccggcg ctgtcgaaac cggcgctgag cgccggggta
    10801 cccacctgct gggcaaccac gtccggtgca accggcgcgg cgtccttggc ggcctcgacc
    10861 gcagcggcgg tggcggcagc ttcgaccctg gcttcaacgg cctcggcttc ggcaaccgcc
    10921 tcatcgatcg cgtctgcatc ccgcagcggc cagtagccga gcaactcgag gctgccgcgg
    10981 gtcttttcga gcaggtcctg acgctggcca tgcggctcgc gcagggattc caggtagtac
    11041 tccatgccgg ccagtgcatc ggccagggtg tccagctggc ggccgtccgg gacctgcagc
    11101 ttgccgatca gttctgcatg gctgtagcgc cgcaatgcct cgaggtagcc ggcaggctcg
    11161 ggcaactgca gcacccgcat cgcgccgccg acctcaccca gcaggcgcgg caccgcatcc
    11221 agcgcggcgt ggtcccaatc ggtttcgacg aaggccacga acgcattgcg cacttccgcg
    11281 aagttggcga ccgcttcgcg cgcgagcacc gcgatcacct tgcgcgattc ctgcgccagc
    11341 aggttgtcgc ctgcgtcgtc gtcgccctgg cccaggcgcg cgacctggtc gtccagcgaa
    11401 tggtcgatgt agatcagcgc accggccacg tccagcaggt cggcttcgtt cgcgggacgc
    11461 tcgccgctgg cgatgccgtg cacgatcttg ccctgctcct ggatcaggct gcgggcggac
    11521 tccagtccga tcatgccgag ggtttcgccg atccggtcca gcgcctccgc ttgcggctgc
    11581 agcgcggcaa cgtcggcgag gccggagcgc aggtgcaggt ccagcgcgtc cttgacccgc
    11641 agcaggtctt ccttgagcgc cgcggacacc gtgtccagca acgcccggtt gcgcccggcg
    11701 aggctgccgc gcgcgtgctc gagttcggcc tcgctgggct cctccagcag ggacggcagt
    11761 gcgtcctggt tgagcgcggc ttcaccgcgg gccgcgcgca gctcgttgag cagcggcagc
    11821 agcacgatcg ggatgtcgcg atgcccgccc tgcaggcgct ccaggtagtc cggcagctgc
    11881 accacgccgc gcatcagcgc cgcgcaagcc tcgtcgcgat tgccggtggc gccgccctgc
    11941 agcgcacccg ccaggtgctc catctcctcg gccaccatcg ccggcgcatg cagttccagc
    12001 atgcgcaggg tgccaagcac ctggtgcagg tagccggcgc aggcgcgcat gcgctcgctg
    12061 tcgccgggag actcggcgaa ggcctcgatc tcgatccgcg cctggcgcag ggtctcgtcg
    12121 atctcgggct tcacccagcc gagcgcggtg tggtcgatgg catcccgcaa cgccgtggtc
    12181 atcgcgacgc tccggtaatg ccccgcaacg cggggcgagc cttgacggtg ctgttcatcc
    12241 gaacctcgac gtcagcggat caggccggca gcttgaagtc ggcgaccgag cggcgcaggt
    12301 cggcggcgag ctgggccagg ataccgaccg actccgccgt ctggctggcg ccctgcgagg
    12361 tctgcgcggt gatcgactgg atggtgttca tcgtctgggt gatgccggtt gccgcgctgg
    12421 actgctgcgc cgccgcggtg gagatgccct gaatcagccc ggacaggtcc gccgacacct
    12481 tttcgatctc gcccagcgcg gtacccgcgt cctcggcgag gcgcgcaccg gcgaccactt
    12541 ccgaggtcgt ctgttccatc gagctgaccg cttcgttggt atcggcctga atggtctgca
    12601 ccagcgattc gatgcgcttg gtcgcgctgg aggagcgttc cgcgagtcgt tgcacttcgt
    12661 cggccaccac cgcgaacccg cggcctgcct caccggcaga tgccgcctgg atggccgcgt
    12721 tcagcgccag gatgttggtc tgttcggaaa tgtcgttgat cagttccacg atcgagccga
    12781 tttcctgcga gctttcgccc aggcgcttga tgcgcttgga ggtttcctgg atctggtcgc
    12841 ggatgctgtc catgccggcg atcgtctgcc gcaccacgcc cgcgccgttg gtcgcgatct
    12901 gcaccgagcg ctgcgccacc tcggcggagt ccgcggagtt gcgcgacacc tggttaatgc
    12961 tggccgcgat ctcgttgatg cggtcggtcg cggagttgat ctcctgcgcc tggtgttccg
    13021 ccgcctcggc gaggtgcatg gcggtggcct gggtttcctg ggcgctggac gcgacctgca
    13081 ccgaggtgtc gttgatcgtg gtcaccaggg tacgcagctg ttccaccgcg aagttgatcg
    13141 agtccgcgat cgcgccggtc atgtcctcgg tcacggtcgc cttcacggtc aggtcgcctt
    13201 ccgcgagcga acccatttcg tccagcaggc gcatgatcgc ctcctggttg cggtcgttca
    13261 gttccttggt ggtctggtag cgcttgcgct ccgcgctgcg ggtgctgaag tacaggccga
    13321 ccagcgacag caggatgcca aggccggaca ggatgctgac ccagacgccg ccgacgatgc
    13381 tggtgtcctt gagcgaaccg aacgaggtca cgctggccag caggcgctgg ctttccgaca
    13441 gcagcttgtt ggaaccggag gccagcgccg ttgccgacgc ctgcgcacca agcaggttgc
    13501 gcgaactggc gttgagggcg tcgaggtcct tcttcatgcc ctgccacagg gtgaaggcct
    13561 ggttgagcgg ggccaccgcg ccggccgcgg tgacgcgggc gatgtcgccg ccgccattgc
    13621 gcaggccgtt catcgaacga tcgaacaccg cggcgtcgcg ggcaaggccg tccgccgcca
    13681 cctgggcacc ggaaccgccg gcgcgcaatt cggcgatgcg gcgggcgatc gccgccgacg
    13741 tcaccacctg ctgcagcgcg gtgaacacct ggcccgaacc cgcaccgccg gaggcaaggc
    13801 cgcgcaccac ttcgttcagc tgcgcctgca gctgcggcat cttcgcggtc acgctgtcgg
    13861 cctggccggc cagcgccagc acctgcggct ccgccgcaag cagctgctcg gcgcccttcg
    13921 ccagcggcgt ccaggtgtcg gtcaccgcct tgatcgggcc ggagaccccg gtggcatcgc
    13981 cgaagttgtc gttcagcgac ttcacgtcgc cgtcgatctg ctccttggtc gacttgaacg
    14041 ccttgtagga atcggcgtta cccgacaccg cgtcctgtgc ctggacagcg agccgctgcg
    14101 agttcacctg caggttcgag gccgcggtac tggcgccacc gacgcgcgcc gccttggtgg
    14161 tggcgtacag ggtatttgcg ccaaacacga ccagcgacag gatcaggatc gctgcccaag
    14221 cacgcgtgct gaggttttgg cccttgccgg cgacggaatc catcatagtg ctcatgttgc
    14281 gacctcggct tgcgtgcttg gctggtgtgt ttggccgccg atcaggcggc ggcttggcgg
    14341 aattcggggg tgcgcgccag gcgctcgagg ctgaacacgc cccagtcctg gtcgttgatg
    14401 cggtaggctc gatcgatgaa atacgcgtag cggccctcgg tcaccgccgc cgcgtcgatg
    14461 ccctgggcat ccacgaagct gcgctggccg tacatttcct cgatggtcac ggcgacgtcg
    14521 ccgcccggct gccgcaccac cagcacgcgc tggccctcgt gcaggacggt acgctcgcct
    14581 tccaggaact gcttgaggtc gaccactggc agcagggtgc cgcggacgtt ggcgacgcca
    14641 agcatccacg gctgcgaacc ggggaccggg gtgaccgccg gcatcgacag gatctcgacc
    14701 acctcgtcga agcccgaggc caaccggtgc gacccgatgc ggtaagccac gccgcgccac
    14761 aggccggggg catcgagctg ctcgggcagg ccggcgacat gcgccaggct gcgctgctcg
    14821 tactcggcca ggaccaggta cggcgagcgc ttgtcgcgcg cgcgcaattt ctgctggcgt
    14881 gccggcttgg cgggtttggc gggtgcgagc tcggcttcgg cctcaggtgc gggcgctgcc
    14941 tgcacgacgt cggcaaccga cgcttcctcg gcaatcgggg catccgccgg cgcatctgcg
    15001 cctgcagcct ccaccgcggc cgcacttccg gcttcgacgt cgaccgccgg cgcctccgcg
    15061 tccagcgtct ccgccgcaac cagggactcc accggagcca cctgcgccgg agccgcctgc
    15121 tgcggcttgc ccttgccctt gttcttgccg cgcttggcca tcggtcagtg cccaaccagt
    15181 gcgttgatcc gctcgatcag gtcggtctcg cgcggcggct tgacgatgta gtccttggcc
    15241 ccctggcgca acccccacat cttgtcggta tcgaggttct tggtgctgac gatcagcacc
    15301 gggatgtggc tggtatcggc atcgcgcgaa agcgcgcggg tcgcctggaa accgttcatg
    15361 cccggcagca ccacgtccat caggaccagg tccggcttgt cgcggcgcgc ggcggcgatc
    15421 ccgtcctcgg cactgcccga cgtcgtgacc tggtgcccgt tgcgttcgag caactgggtc
    15481 atgaccgcgg cctcggtcgg tgaatcctcg atcagcagaa tgcgtgccat gcgtgcctta
    15541 ccccccggtc aggcgttgac gtacgtgcgg attgccccga gcagttcttc ccgcgtaaat
    15601 ggcttggtga ggtactgctc ggacccgacg atacgtcctc gggccttgtc gaacaaaccg
    15661 tctttcgacg aaagcatgat gaccggcacg gatttgaagg tctggttgtt cttgatcagc
    15721 gcgcaggtct gatagccatc caggcgcggc atcatgatgt cgacgaagat gatctgcgga
    15781 tcgtggtcgg cgatcttggc cagcgcctcg aaaccgtcgg tggcggtgac cacgtcgcaa
    15841 ccctcgcgca ccagcagggt ctccgccgtg cggcggatgg tcttggagtc gtcgatgacc
    15901 atcaccctca atcccttgag gctgacgtcc tgggcgtcct gcgtcatgcg ttccccttga
    15961 tccgctgagc gccgccacgg gattcgccgt ggcgcggact gcctatatcc cagtccgtgg
    16021 acgacctgtc aagcgccgca ttgcgtcacc ccgaagatcg ggccgggtgc cctgctacca
    16081 tcggccctgc attcccggga gctgccgatg cccgctgtag caccactgga cgtggtggtg
    16141 gtgatggatc cgatcgaatc catcaagccc gccaaggatt cgagtttcgc catgctgctg
    16201 gaagcccagc ggcgcggcca tcgcctgcat tacgtgcgcc cgggcgggtt gtcggtctct
    16261 ggcgcccgcg cggtggccac cgtagcgccg ctgggcgtgc gcgaccaggc gaacgaacat
    16321 ttcgaactgg gcgcgtggtc acgcctcgag ttcgggcccg ggcaggtcgt gctgatgcgc
    16381 aaggacccgc cggtcgatgc cgattacctg catgacaccc agatcctcgg catggcgcgg
    16441 ttgcagggcg cgctggtggt caacgacccg cagggcctgc gcgatttcaa cgagaagctg
    16501 gccgcgctgc tgttcccgca gtgctgcccg ccgaccctgg tctcgcgcga cgcgaccgcg
    16561 ctcaaggcgt tcgtcggcga acatggcgag gcagtgctga agccgctgga cgggatgggc
    16621 gggcgctcga tcttccgcgc ccgcgcgggc gaggccaacc tcaacgtgat cctggaaacc
    16681 ctgacccagg gcggcgccca cctggcgatg gcgcagcgct accttccgga gatcgtggcc
    16741 ggcgacaagc gcatcctgct ggtcgatggc gagccggtcg actactgcct ggcgcgcatc
    16801 ccgcagggcg acgaattccg cggcaacctg gccgccggcg gccgcggcga aggccgaccg
    16861 ctgaccgagc gcgaccgctg gatcgcggcc caggtcggtc ccgagatgcg ccgccgcggc
    16921 atgctgttcg tcggcctcga cgtcatcggc gactacctca ccgaagtcaa cgtgaccagc
    16981 ccgacctgca tccgcgagct cgacgcccag ttcggcctgg acatcgccgg caccctgttc
    17041 gagcgcatcg aagccaagct ggcgtcctga tgggcgagcc gcgcgccgct gccgaggccg
    17101 tggtcctgcc cccgccgcca cgggtcggcg aatccgagcg gctcggcgcg accatggtgc
    17161 tgtcggtgct ggtgcacgcg atgctgatcc tgggcatcgg cttcgcggtt gaggacgccg
    17221 cgccgatgat gccgaccctc gacgtgatcc tgtcccagac ccggaccgcg ctgacgccga
    17281 aacaggccga cttcctggcc caggccgaca accagggcgg cggcgagcac gacaagagca
    17341 gccgccccac cgcgccgcag gcaggtccgc tgccgcggcc ggaggacggg ctggcgccgc
    17401 gcccgctgcg tgcgcagacc cccgcgccca gcccgccgcc ggaaacgcgg gtggtgactt
    17461 cgcgcaacgc gcggcaggtg ctgccgacgc cgcagccgcg gcaggaaatc gaggccagcg
    17521 tcctgccgcc gggcagcgag aagatcgacc gcgacatcga aatggcgcgg ctcgccgcgg
    17581 aaatccagtt gcgctcgcag cagtacgcca agcgtcccaa gcgcaagttc gtctccgcca
    17641 gcaccaagga atacgcgtgg gcgcagtacc tgcgtggctg ggttgaccgg gtggagcgcg
    17701 tgggcaacct caactacccg gacgaagcgc gccgccgcag gatcggcggg ctggtggtga
    17761 tcagcgtcgc ggtgcgccgc gacggcagcg tggagagcac ccgcatcatc cagtccagcc
    17821 acgtgccgat gctggacagt gccgcgctgc gcatcgtcaa gctgtcggag ccgttcgcgc
    17881 cgctgccgaa gacccaggac gaccccgaca tcctgcacgt gacccggacc tggcagttca
    17941 tgccgggcgg cgagctgatc gaccaatagc tcccggcagc ggcgcgtcag cgggaagcgc
    18001 gcaatgcctg ttgttcggcg atggtcagcg ccacgttgtt gcgcaggtag gccggctcga
    18061 tgcgctccgg cgcgaccgcc ccgccgcgcg cgaacagcag cgcgccgagg cgggctacgt
    18121 ccgccgcatg cggcagcgct tgcggatcga ccgtggcgag gcggccctgc atccgtgcct
    18181 gcaacgcatt gtcgccggcg gcgaagccgg tgccgacacc gtgccagcaa tcgcccgcgg
    18241 ggaggatcgc ggcatccggc ttgaccacga cctcctcgcc caatgcgacg acatcgtcgc
    18301 cagcgcgggc gaaggcggcg aggtagacct cgcccatccg cgcgtcgatc gcggcgacga
    18361 tgcggccgcc ttcggcgcgc atcgccagcg cggccagcgt cgagaccggc agcagcggcc
    18421 gatccagcgc cagcgcgatg ccctgcgcca gcgcgatcgc caggcgcacg ccggtgaacg
    18481 cacccgggcc gcgaccgacg gcgatcgcat cgagctgggg cttggccacg ccagcgtcgg
    18541 ccagcagctg ctccgcccat ggcagggcca gttcggcgtg ccggcgcggg gcgatctcga
    18601 agcgctcacg gacatcgccg tcgaccagga tcgcgacgga gcaggcttcg gtggcggttt
    18661 cgatcgcaag cagtctcatc gctcaattat ccgcgaagaa ggcctgcacg tcggcgagtt
    18721 cacgcgtgcg cgggatcggc ggcagcgact ccaggaacac gcggccgtag ctcttctggg
    18781 tcagccgcgg atcgcacagc accagcacgc cacggtcgcg ctcggtgcgg atcaggcggc
    18841 cggcaccctg cttgagcgcg atcaccgcct gcggcagttg ctcgtcgcgg aacgggttgc
    18901 cgccgctgcg gcggatcgca tccaggcgcg cttcgaacac cggatcgtcc ggcgcggcga
    18961 acggcagctt gtcgatcacg accaccgaca acgcatcgcc ggccacgtcc acgccctcgc
    19021 ggaaactcgc ggtgcccagc agcacgccgt tgccggactc gcggaaacgc tgcagcagca
    19081 catgccgcgg ctcgctgccc tggacgaaca gcggccacgg cccgtcgcgc aggagttcgg
    19141 cggcctcgcg cagggcacgg tgcgaggcga acagcaggaa cgcgcgcccg gccgaggcgt
    19201 gcagcaccgg cagcagcgcg tcgacgaggg cggcgccgaa atcgcgcgcg gccggttccg
    19261 gcaaccgcgg cggcaggtag cacagggcct gccggtccca gtcgaacggg ctgggctcga
    19321 tcagttcgac cgccgtatcg atgcccagcc gctgcgcgat gtgcgagaag ccaccggcga
    19381 ccgccagcgt ggccgaggtg aatacccagg cggcgtgcga ggcatcccga tgcgcgcgca
    19441 atggcgcgga cacgtccagc ggcgtgcgct gcaggcggaa gccgcgcggg gtgagttcgt
    19501 accagtgcac ggaaacgggg tcagggccac ttgccgccgg cacgtccgcc gacctcggca
    19561 tgcctgcatc cggacgtgga cctgatccgg cttctcccag ccagcggcgc agccggccga
    19621 gctggtcgtt cgcgcgcagg tggcaggcat cgaagccggg cgcggcttcg cgcagcggct
    19681 gcagcgcctg ctggagcgcc tgcagggcat gctccagcgc ctggaacccg gcctcgacgc
    19741 cttcgccctc cagcgcgcgc cagcgggtgc cgcgctgcgg caggtggtcc atccctgcac
    19801 gcagtgcgcg cgccgcctgt tcaagcatgc gtgccggttc ctgcaggctg cccagcgctc
    19861 ccggcacgtc cttgcattcg gcgatcgcgt cgcgtgcaag ttcgaccagc ggccgcgcgc
    19921 ccaggccctc gccgaagaac tgcgcggcaa gttccggcag ctggtgggcc tcgtcgacca
    19981 cgaacgcctg cgcgcccggc aggatttcgc cgaagccttc ctgcttgagc gcaaggtcgg
    20041 ccagcagcag gtggtggttg accaccacca ggtcggcggt ctgcgcgcgc tggcgcgcct
    20101 gcaccacgaa gcagtcgctc caggaggggc actcgctgcc caggcagttg tcggcggtgg
    20161 aggtcacctg cggcagcaag gggctgtctt caggcagcgc gtccagttcg gccatgtcgc
    20221 cgaacttggt gcgcccgctc caggcgacca cgcgctggaa ctgcgcgacc tgctcacgcg
    20281 aactgaagcc gcccttggac agctgcggat cgcccttcgc ctgctccatc cggtacaggc
    20341 acaggtagtt cgagcggccc ttcagcaggg ccgtcttcag gccgatcccg agcgcgtcgc
    20401 gcacgcgcgg caggtcccgg aaatacagct ggtcctgcag tgcgcgggtg ccggtggaga
    20461 tgatcgtctt cattcccgac agcagcgccg ggaccaggta ggcgaaggtc ttgccggtgc
    20521 cggtgccggc ctccgcgagc agcacgccgc gctgttcgaa ggcctcggcc accgccatcg
    20581 ccagccgctg ttgcgcgggc ctggccacga agccggggat gccctgcgcc agcgccgcgc
    20641 cttcggacag cgcatcgcgg gcgcctgcac ccaggcgcga ggtcgagatc gggtccacca
    20701 ccggctcagg gttgcggcac gggatcggcc ggcggcgcgg gttccggtgg cgcctgggtg
    20761 gccgggtcgc ggccatcgcc caagccgagg aagtcgcgga tcgcctggcc ggcacgctcc
    20821 agtggcgcac tgccgtcgtc gggtggcaac gggttgccga attcgtcgaa cgcagggggt
    20881 ggcgcgtaca cgcattgccg gtacgtcggc gcgtagccgg ccacgaaggc gaagcggcgc
    20941 gcacccgggc agccgggatc ggtgctgtgg ccgccctcca cccactgcca gtccaggccc
    21001 tcgcccggca cccgcagggt tgcgctgggc aggcgcgcga agatgtccga ccacacccgc
    21061 atgccgccgg tggcgccgta caggccggtc ggctggttct ggtcgttgcc gacccagacc
    21121 accgccaggt ggtcgccggt ataaccggcg aaccagctgt cgcggctgtc gttgctggtg
    21181 ccggtcttgc ccgccggcgc caaccggccg agaccgtcgc gcagcagcgg acggccggtg
    21241 ccggaggtga ccgcgtactg cagggcggtg ccgaccagcc gcgccgccac cgcatcaccc
    21301 ttctgcgccg gcggcggtgc gctgtcgtag cgcttgatcg tgcggccctg cggatcgagc
    21361 acgccgcgca cggcatgcaa cggctggatc tcgccgtcgg cggcgaggaa ctggtagagc
    21421 tgggccatcg cgtacgggct ctggtcgacc gcccccagga tcagcgaggg ctggccctcg
    21481 gacgtgatac ccgccagcgt gcggatcagg tccgccagcc gctgcggttg cacgtccatg
    21541 cccagtcgca ccgtggcctg gttgtagctc tgcgcgagcg catcgaccag gcgcaccgtg
    21601 ccgtggctgc ggttgtcgga atttcccggc ttccagtcgc ggccgcggcc gaggctgacg
    21661 gtcaccggcg catcgctgat ggtgctggcc agcgaccatt tgtccggctg cgccagcgcc
    21721 agcaggtaga cgaagggctt gagcagcgat ccgaccgggc gcctggcgtc gaccgcgcgg
    21781 ttgaagccct gttcgccgaa attgcggctg ccgaccaccg cgaccacgtc gccattgtgg
    21841 acgtcggtca tcaccacgcc tgcctgcagc ggtggccggc ccttgccacc caccgatttc
    21901 agggtgcgcg cgaccgcgcc ctcggcatag ccctgcgcgg acggcgacat gccggtcatc
    21961 acgctgaggc cggccccgga gatcgaatcc gccgggtagt cgcgcgccag ctgcttgcgc
    22021 accaggtcga cgtacgacgg gaagcggttg gcggcgacgt tgccggcgtt ttcggtgatg
    22081 cccagcggcg ccgtggtggc gcgcttgaac tcggcatcgt cgatcaggcc gctttcgtgc
    22141 atcttgccga gcacgaagtt gcggcgctcg gtcgcgcgcg ccgggttgcg ccgcgggttg
    22201 taccaggacg gccccttgac cagcccgatc agcagcgcga cctgctcggt ggagaggtcg
    22261 cgcaggtcgc ggccgaacca gaattcggag gcggcggcga ccccgcggat cgcctgcgcg
    22321 ccgcgctggc cgaggtcgac ctggttgaaa taggcctcga ggatggtgcg cttgtcgtag
    22381 cgcgcctcca gcagcagggc gaacaggatt tccttgaact tgcgggtgta ggtctgctcc
    22441 ttgccgatcc ccagcaggcc gctgcgggcc agttgctggg tcagggtgct ggcgccctgg
    22501 cgggtgtcgc cgccggactt cacgttgacg aacagcgcgc gcagcatgcc gctgaaatcc
    22561 acccccaggt ggtgggcgaa gtcgcggtct tccaccgcct gcaggccggt gaccagcagt
    22621 tccggcactt cctcgatccg caccagccgg cgttcttcct gctgttgccc gtacagggtg
    22681 gcgatccgcg ccgggtccag ccgcgcgctg cgcagcgcct tgcggctgcc gaggtcgcgg
    22741 atcgcgatga cttcgccgcc ggacagggtc acttccagcc gcgccggtgc gatccggcca
    22801 tcgacgtcgt cgtagccacg gctggcgatg tgccagcgac tgccgttgcg tgcgtaggtg
    22861 ccggcgcgct tgccatcgcc accgtggtag gacgcggcgt ccagctcggt cttgagggtc
    22921 tgcgcatcca tcgccaggcc gtcgcgcagg gtcagcggcc gcgcgtagac ccgggtcggc
    22981 acctgccagc gcagcttgcc gaagcgctcg ccgacctggt ggttcaggta cagcatgtac
    23041 gggaccagga agcccagcag cagggcggcg gcagccaggc cccaggtcaa cagccgcgac
    23101 cgccagtcga tgccgccctc gccgccttcc cgatcctcgt cgtgcaggtc gtcgtcttcg
    23161 tcttcgatgc ggggcatggg cggtctggct ggcggcggtt tcgcaggatg cgacaatgcc
    23221 gggaacggtc gtggcgagtc tagcgcaggg gttgcgcgct gcgcggtagg ccgtcgcccc
    23281 cttcgtcagc cagaatttac cagccggagt tccgccgcat gcccatcccc gccgaggcgc
    23341 gcttctggat cgcgcgcgcg ctgggcctgt ggcggcgcgg ctgggccagc ctgcgcacgc
    23401 gcgggctggc ggccagctgg cggcgcctgc agctgcattt ccggcgcccg gcggtcccgg
    23461 cactgccgct gtacgcgccg gacgcgttgc cgttcgcgcc attcgcgatc gcgacgtcgg
    23521 acgccccgcg cgcgagcatc gtggtgccgg tccacggtgc gttcgcgcat accctcgcct
    23581 gcctgcgcgc gctggccgcg catccgccgc gcgccgcgtt cgagctgatc gcggtcgacg
    23641 atgcctcgcc cgacgacagc ctggcgcggc tcgcgcaggt cggcggcatc cgcgtgcatg
    23701 cgcgtgcgcg caacggtggc ttcatcgccg cctgcaacga aggcgcggcg ctggcgcgcg
    23761 gcgactacct ggtgttcctc aacaacgaca cggtgccgca gcccggctgg ctggacgcgt
    23821 tgctgggcac gttcgatcaa cacccgggca ccggcctggc cggcgcgaag ctggtgtacc
    23881 ccgatggccg cctgcaggaa gccggcgggg tggtgttcgc cgacggatcc ggctggaact
    23941 acggccgcaa cgattcgccg gacgattgcc gctacgcctg cgtgcgcgct gccgactact
    24001 gcagcggcgc cgcgatcgcg atcccgcgcg gactcttcga gcgcctcggt ggcttcgacc
    24061 agcgctacgc acccgcctat tacgaagaca ccgacctcgc cttcgcggtg cgcgctgccg
    24121 gcctgcgcgt gctctaccag ccggcggcgg tggtggtgca cgacgaaggc gcgaccgccg
    24181 gcaccgacct ggcgcagggg ccgaaggcgg cgcaggtgcg caaccagtcg gtgttcgcgc
    24241 acaaatggca ggacgcgctg cgcacgcagc tgccggcagg cagcatcccg acgccggcgc
    24301 tgctgcatcg cggccagccg caggtgctga tcgtcgatgc actgaccccg cagcccgatc
    24361 gcgattccgg ttcgctgcgt ctggtcaacc tgatgcggct gctgatcggg gaaggcgcgc
    24421 acgtggtgtt cctgccggcc aaccgcagtg ccgacggcgc gtacacgact gccctgcagc
    24481 agctgggggt ggagtgctgg cacgcaccgt tcgccaccgg catcccggcc tggctgcgcg
    24541 aacacgggcc gcgcttcgac gtggcgatgg tcagccgcca ttacgtcgcc agcgaactgc
    24601 tgccgctgct tcgcaagcac gcgccgcgcg cgcgggtatt gttcgacacc gtggacctgc
    24661 actacctgcg cgaacgccgc gccgccggac tgtcgggcga tgccgccgcc ctgcgcgctg
    24721 cgcagcgcac ccgcgaggcg gagctggcgc tggtggcgca ggccgatgcg acgctggtgg
    24781 tgagcgaggc cgaacgcgcg ctgctggccg aggatgcacc gggcgcgcag gtgcgggtgc
    24841 tgtccaacct gcacgaaccc ggcgcggacg gcctggcctg gtcgcaacgc cgcgacctgc
    24901 tgttcgtggg cggcttccgc cacccgccga atgtcgatgc ggtgctgtgg ttcgtgcgcg
    24961 aggtgttccc gcgcatccgc gccgcccgcg gcgacgtccg cttccactgc atcggtggcg
    25021 atgttcccgc cgaggtgcag gcgctggccg ccagcgatgg cgtccagatc cacggccacg
    25081 tcccggacct gcagccctgg ctggccggct gcctggccgc ggtcgcgccg atgcgctacg
    25141 gcgccggggt caagggcaag gtcaaccagg cgatggcgca cggcctgccg gtggtcgcga
    25201 ccacgcccgc ggcggaaggc atgcacctgg tcgatggccg cgacgtgctg gtcgccgacg
    25261 ccgcggaggc gttcgccacg gcggtactgc gcctgcacca ggacgaggcc ctgtggacac
    25321 ggctggcggc gaacggccgc gccagcgtgg cgcggcattt ctcgatggac gccgcgcgcg
    25381 gcgtggtgcg cgagctgttc ctgcactgag tcgtggacgc ggcgtgccgc tgccggccgc
    25441 cagagccgtc gccgacgcgt ggttgcgaag ccgggtccgg gcgttcgccg gcgcggtggc
    25501 ggtcgctcag ccgcggcgcg cggattcgac cccggccatc gcttccagcc gccccagcaa
    25561 cagcccgagc tggccatagt cggccacccg cacgcgcacg ttcaggcgca cccgcgggtc
    25621 gccatcgcgc agctggtcgg cgtgcaaacc cagcacatgc acgccctgct gggcgagcag
    25681 ggtggtgatg tccttcagca gccatttgcg gtcgaccgcc tcgatccgga tcgccacctc
    25741 gtggccgccg ctgcgcgcgc cccactccac cggcagcacc cgcgccggct gcctgccggc
    25801 caggcgcagg aacgacgcgc agtcaggccg gtgcacgctg accccgcgcg cgcgggtcag
    25861 gtaaccggcg atcggctccc ccggcaacgg ctggcagcag cgcgcgacct gcgccagcag
    25921 gttgtccacg ccctgcacgg tgaccgtacc ggcggcgggc gtggtcgcct tcggcggcgc
    25981 ggtggacggc aacggcggtg cttcttccgc cggcttctcc agctccagca gggcgcgccc
    26041 gacctggttc gggccgacat cgcccagcgc caccagcacc agcagttcgt ccaccgtggc
    26101 cacgttgaac ctgcccagca ctggctgcag gtcggcgtgc agcagtccga gccggcgcag
    26161 gtcgcggtcc agcagttcgc gtccggcctg caggttgcgc gcacggtcca gcttgttgaa
    26221 ccaggcccgc accttctccc gcgagcggtt gctggcaagg aagccggaac tggcctgcag
    26281 ccagtcgcgc cggggttccg cggtcttgcc ggtcaggatc tcgacgcgct cgccgctgcg
    26341 tagctgggtg ttgagcggca cgatgcggcc atccaccttc gcgccgcggc agcgatggcc
    26401 gaccatggtg tgcacctggt aggcaaagtc gagcacggtc gcgccctgct gcaggtcgac
    26461 cacttcgcct ttcggcgtca gcgcgtagac gcgatcctcg accagctcgc tgtccatcgc
    26521 gtccagcggc gacgcctcgc cgtcgcgcgc ggcctccgcg gcctgctcca gcagcttgcg
    26581 catccacgcg atgcgcttgt ccatcgccgc gccgccggcc gcgccgccct ccttgtagcg
    26641 ccagtgcgcg gccacgccga gttcggcctg cgcgtgcatc tcgcgggtgc ggatctgcac
    26701 ttccagggtc ttgccgccag gcccgaccac cgcggtgtgc agcgagcggt agccgttgct
    26761 cttggggcgc gcgatgtagt cgtcgaactc gctcgggacc ggcagccaca gtgcgtgcac
    26821 caggccaagc acggcgtagc agtcggcgac atcctccacc agcacccgca gcgcgcgcag
    26881 gtcgtacagc tcgccgatcg gcgcatcctt gcggcgcatc ttcttccaga tgctgaagat
    26941 gtgcttgggc cgcccggcga tgtcggcgtt ggcgatcccc tgcgccgcca gcgcttcctg
    27001 cagctgcgcc ttcgcctgct cgatgtagcg ctcgcggccg ctgcggttgt cgtccagcag
    27061 gcgggcgatg cgcaggtagg tgtcggtttc caggctgcgg aacgccaggt cctccagctc
    27121 ccacttcagc tgccagatgc ccagccggtt ggccagcggc gcatggatgt cgcgggtcaa
    27181 ctgcgccagt gcgcgctgct cctccaccgg caggcgccac gccgcgcgca tgcgcgacag
    27241 ctggcgcgcc agcacgatcg gcaccacccg caggtcgcgc accagcgcca gcagcaaccg
    27301 gcgcatgccc tccgggttgc ggccaccgcc gtggctgcga tgcagcgccc acacccggct
    27361 ggccgcacgc tggccctcca gcagcgcctg caggcgctcg gggatcgcca cgccctggcc
    27421 ggcggcgctg cgctcgacat ccaccagcgc gtccagcagg gccgcggcga ccacgtcggc
    27481 atcggcattc aacgaggcca ggatgcgcag ggtgtcggcc agcacgtcgg cgcggtcgcg
    27541 catcggcgca cccgcctccg cctgcgcgct ccacagcgcc gccagcggct ccggcagcgc
    27601 gcccgccggc aggcgcgaga gcgcatcggc gtagtgcatc ttggcggcgg caacgggcgc
    27661 aggcatggcc ctacagtagc gacgccggcg cgctgcagcc agccatgcag cggcatgccg
    27721 tcatggtcgc tgcagccgtt gcaggcgggc cgccaggcgc ttgggcgaaa ccccgccctt
    27781 gactcccagc tcctgcgcga acagctgcac gcgcagctcc tgcagttccc ggcgcaaggc
    27841 ctgcgcgtcg gcatcggccg gatccgccgc cgccagcgca tcggcgaacg gcttgagttc
    27901 gagcatgcgc gcctggtcgc gggtcgggtc gcgttgcgcg cgctcggcgc gcagcaccag
    27961 cgccttcagc cagcgcggat actcggccag cacctccgcc gggacctcgc gcaggaagcc
    28021 cggcgcagcc aacgccgcca gctgtgcctg catgtcatcg aggttggcgc gcgcccagcc
    28081 catcagcttc gactccagct tcgcgcgcac gtccgccacc agcccgagga tggtgtccgc
    28141 ctgccgcagc cgctgcatgg cttcgccgaa cagcgccttg gcgagcgcgt cgcggcgctg
    28201 cgcgaaggcc tccgcatcgc ggatcgcgag caggccgtcc gcggccagcg cggcaaacgc
    28261 accatcgacc aggtccgagc gcaggcggtc cgcatccttc agaccgtcgc ggcgcgggtc
    28321 cacggattcg atggccgcgt aaagcagcga aatcttcggc tgcaccggca gctgcttgcg
    28381 cgcctgtttc agcttgtctg ccagcgcgat cgccagcagc cggcgcacgc cctgaggatg
    28441 caggcgctcg gccacctcgc gctgcgcgtg cagggccagc gagacgctgt cgccatcgtc
    28501 ctgcaacgcc ggataggccg gcacgccgcc ggcgcccggc accgacacgg gaaccggcgc
    28561 ggccggaaaa ccggtcatgc ctgcttgcgc catgccctgc gcggcgtgac gtgcgaacgc
    28621 ctgcgcggcg cgctcgccga agcgcgcgcg cagttcggcg agatcgcgcg actcggccag
    28681 tactgccttg ccatccagca ggcgcaggtt ggcgcgcagg tacggcgcga tggcggcggc
    28741 atcgaaatcc agcgcggaca cctccacccc ggagagcttc ttcaggaagc gtgcaagcgc
    28801 gccctcgatg ctgtccggct ccaccgttgc gtgcgcctcc gcgaaggcgc gggcgaaatc
    28861 cggtgccggc acgaagttgc gccgcaacgc cttcggcagc gagcggatca gcgccaccgc
    28921 cttgtcctcc acgaagcccg gcgccagcca cgacaggcgg gcggcatcca gcgcgttgag
    28981 cagatgcagc ggcagcgcca cggtcatgcc gtcgtccacc gcgccgggct cgaagcggta
    29041 ttgcaccgcc aaccgcacat cacccagcgc gagatacgcc gggaagcgcg cggcatcggt
    29101 ctggtcggcc accagcaggt cgtcgcgcgt ccactccagc gcggcctttt cgtccttcga
    29161 cagtttgccg aaccacgcat ccagcgcctg cgcgttgtgc acgtgcgctg gcagccggtc
    29221 caggtaccag cgcgcctgcc agtcctcgtc caccaccagg ccgacccggc gctgcttggc
    29281 ctcttcctgc tgcgccagcg ccagcgtgcg caggttgcgg gcgaggaagc cgctgcgggt
    29341 gttgatctcg ccggccagca gggcctcgcg cacgaagatg ccgcggctct cttccgggaa
    29401 cagcgcgccg taatggatgg gccgcttggg tgccagcacc aacccgaaca ggctgatctg
    29461 ctcgctaccc agcacccgtc cctgcgcacg cgaccagtgc ggatcgtggt ggcggcgcgc
    29521 cagcaggtgc ggcagctcgg cgatcaccca gtccggttcg atgccggcgt tggtgatcgc
    29581 ccagactttc tcggtgtcca gcaggttggc cgacagcacc cacggtggcg gcttcgacgc
    29641 cagccttgag ccggggaaca gctggaactt gcgcccgcgc gggccgtcgt agagaccctt
    29701 gtcaccacga ttgccgatct gggtgggcaa gccggcgatc agcgcgcgat gcagttcagc
    29761 aaagccgcct ggttcatcgt tctgctgcca gcccagctca tcgcactgca gcttcaactg
    29821 tcgatgcaat tcccaccatt cgcgcaggcg caggaagccg aggaaatgct tgtcggccca
    29881 tttgcgcagc tgcgactggg tcatgtcctc gtgcgcttcg cgataggcct gccacagctt
    29941 gaggatgccg atgaattccg actttgcatc ggcgaactgc gcgtgcgcgg catcggcggc
    30001 gccgcgggcg tcggcagggc gctcgcgcgg gtcctggatg ccgaggaaac tggcgatcac
    30061 cagcatctcg cgcagcacgc cgtgcacctg cgcggccacc agcatgcgcg ccagcttcac
    30121 gtccaccggc agcttcgcca tcgccttgcc gatggcggtg agcttgcggt tggcatcgac
    30181 cgcaccgagt tcgttcagct gctgccagcc gtcggcaacg gcgcgcgaat cgggcggctc
    30241 caggaacggg aagtcttcaa tgctgcccag ccccagcgac agcatccgca ggatcacccc
    30301 ggccagcgcg gcgcggcgga tttccgggtc ggtgaattcg ggccgcgagg cgtagtccgc
    30361 ctccgagtac agccggaagc aggtgccggg cgcgatccgc ccgcagcggc cggcgcgctg
    30421 gttggcgctg gcctgcgaaa catcctcgat gtgcaggcgg tccagcttct ggcgcgggct
    30481 gtagcgcttg acccgggcca cgccgggatc gatgacgtaa tggatgcgcg gcaccgtcag
    30541 cgaggtctcg gccacgttgg tagccagcac gatgcgccgc tgcgggccgg gctggaacac
    30601 gcggtcctgg tccttcgccg acagccgcgc gtacagcggc agcacttcgg tgtggcggta
    30661 tttgcgcttc tccagcgcct ggtgggcgtc gcggatctcg cgttcgccgg gcaggaagat
    30721 cagggtgtcg ccgatgcccc tgtcgcgcat gatctcgtcg caggccgcga cgatgccatc
    30781 gacgatcgtg cgctcgcccg ctccttccaa cgattccgct gccatccgtg gcgaaacttc
    30841 gccgccttcg gatcgccctg cccggccatc cgtggccggg cgttcgccgg tgcggcggac
    30901 aagttgtccg ccggcacgcg ccggctcacc cagcgggcga taacgcattt ccaccggaaa
    30961 cccgcgtccc tccacgccga ccaccggcgc accgccgaag tgttcggcga agcgcgcggt
    31021 atcgatggtc gccgaggtga cgatcagctt caggtcgggc cgcttcggca acagctgctt
    31081 caggtagccc agcaggaagt cgatgttgag gctgcgctcg tgcgcctcgt cgatgatcag
    31141 ggtgtcgtac ttcgacagcc agcggtcggt ctggatttcc gccagcagga tgccgtcggt
    31201 catgaacttg atcgcggtct gctcgccgac cgcgtcgttg aagcgcacct ggaagccgac
    31261 cgcgccaccc agcggcacct gcagttcctc ggcgacgcgc cgcgccaccg agcgcgcggc
    31321 gatccggcgc ggctgggtac agccgaccat gcccgccgcg ccgcgcccgg ccagcaggca
    31381 cagcttgggc agctgggtgg tcttgccgct gccggtttcg ccggcgacca ccaccacctg
    31441 gtgggcgcgg atcagggcga cgatctcgtc ggcgtgcgcg gcgatcggca acgtcgtatc
    31501 cacgctggcc gcaggcaggg cagctgcacg gcgcgcgcgt tccgccaccg acgcctgcag
    31561 cttcgccgcg aagccctcgc gcagggccgg gtcgtctggc tttgcgttcc acttcgacca
    31621 cagcgccagc agccggccgc ggtccttgct caacgcgccg tcgatggcgc gccgggcctg
    31681 ccttgacgcg gtctttccgt tgttttcggt agcttgcatc gatggccgtg ccaggtaacg
    31741 ttgatcacac ggcaacgccg catcccgcta ttgtcgccgc aaccaccaag ggaacatcgc
    31801 catggccaag aagaagtccg cccccaagtc caccgccaag cccgccccca aggccgacaa
    31861 gccgctgccc accgcgcacg ccgacggcgc gccgggcatc gacatcggca tttccaccgg
    31921 cgaccgccgc aaggtcgcgg acggcctgtc gcatttcctg gccgatgcct acaccctgta
    31981 cctgaagacc cacaacttcc actggaacat caccgggccg atgttcaaca gcctgcacgt
    32041 gatgttcgag ggccagtaca ccgagcaatg gaccgcgctg gacgaaatcg ccgaacgcat
    32101 ccgcgcactc ggcttcaagg cgccgggttc atacgcggag ttcgtccgcc tgagttcgat
    32161 ccccgaagaa cccggcctgg gcgactccgc cgactggcgc gagatggtgc gccagctggt
    32221 ggtcggcaac gaagccgtgt gccgcaccgc gcgcaaggtg ctggccaccg ccgacaaggc
    32281 cggcgacgac cccaccgtgg acctgatgac ccagcgcctg aacatccacg agaagaacgc
    32341 gtggatgctg cggtcgttgc tgcagtaggc gaccctgcgg aatcacccca tgggagcccc
    32401 tgccctcgaa caactccgcc acgtcttcgg ttacgacagc ttccgcggcg aacaggccga
    32461 catcgtcgga cacctggtcg ccggtggcga tgccttggtg ctgatgccca ccggcggcgg
    32521 caagtcgctg tgctaccagc tgccggcgct gctgcgcgcc ggcaccgcga tcgtggtgtc
    32581 gccgctgatc gcgctgatgc aggaccaggt ggaggcattg cgccagcttg gggtgcgcgc
    32641 cgccttcctg aattccagcc tggatgccgg cgcggcggcg caggtcgagc gggaattgct
    32701 cgacagccag ctcgacctgc tctatgtcgc cccggagcgg ctgctgacac cgcgcttcct
    32761 gtccctgctg gaacgcacgc cggtcgcgct gttcgcgatc gacgaggcgc attgcgtctc
    32821 ccaatggggc cacgatttcc gccccgaata ccgccagctg accctgctgc acgagcgctg
    32881 gccggacgtg ccgcggatcg ctttgaccgc caccgcggat gcgccgaccc ggcgcgagat
    32941 cgtggagcgg ctggcgctgg aggacgcgcg ccagttcgtc agttcgttcg accgtccgaa
    33001 catccgctac acggtggtgg ccaaggacga cagccgccgc cagctgctgg agttcctgga
    33061 cgaccatcgc ggcgacagcg gcatcgtcta ttgcctgtcg cggcgcaagg tcgatgccac
    33121 cgccgaactg ctggccgggc gcggcgtgcg tgcgctgccg taccacgccg gcctggacgc
    33181 ggccacccgc gccgaacacc agcgccgctt cctgcgcgag gacgggatcg tgatggtggc
    33241 gacgatcgcc ttcggcatgg gcatcgacaa gcccgacgtg cgcttcgtgg cccacctcga
    33301 cttgccgaaa tcgctggaag gctattacca ggaaaccggc cgcgccggcc gcgatgg
//
LOCUS       DAORUK010000004        28451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_1214_length_28451_cov_18.783737, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000004 DAORUK010000000
VERSION     DAORUK010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 28451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28451
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1214_length_28451_cov_18.783737"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PK818_00655"
     CDS             <1..677
                     /locus_tag="PK818_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011050921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY81276.1"
                     /translation="ILIVFALTTPFWSLFDQKASTWVIQGKEMVVPHDAWWWPSWLVK
                     EAGQMQALNPLLIMLLIPFNNIVLYPALRRIGWEPTALRRMGFGIAFAGIAWVIAGLI
                     QLQIDSGAQTSLAWQTWPYLLLTFGEVLVSATALEFAYSQATQSMKGVIMAFWYLTST
                     FGSLWVLLTNVAVRNEAVTARIATTGYSENAFLMFFFAVFAFAAALAFAAYARTYPMQ
                     DNYRAA"
     gene            681..1301
                     /locus_tag="PK818_00660"
     CDS             681..1301
                     /locus_tag="PK818_00660"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQY81277.1"
                     /translation="MDFTLTLAIVAITVLVSWRAFNDRALLERLILWPPAVERRGQYD
                     RLLTHGFIHADWMHLLFNMITLWSFGSAVEQVFSAWITPAGFVLFYLSAIVVAILPTW
                     LRHRRDASYRSLGASGGVAAVLFASILFDPWATLILFPIPVPIPAFVFALLYVAYSIW
                     MDRRGGDNVNHSAHLWGAAYGVLFTLVLEPRILGHFARAVLKSGID"
     gene            1315..1590
                     /locus_tag="PK818_00665"
     CDS             1315..1590
                     /locus_tag="PK818_00665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY81278.1"
                     /translation="MQSDIRHDAASQRFLTEVDGHAAYVEYERCDGVMAITHTIVPAA
                     IGGRGIAAALVRVAVEHARAQGVKVDPQCSYADAWLRRHPEFAELRA"
     gene            1622..2338
                     /locus_tag="PK818_00670"
     CDS             1622..2338
                     /locus_tag="PK818_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016849880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQY81279.1"
                     /translation="MRLNRHIADSGFCSRREADRLVAEGRVTVNGLRARIGAELGEGD
                     EVRIDGNTLEARTAARGQRRHVYIALNKPVGVTSTTDSGVKDNIVDCVDHPQRIFPVG
                     RLDKDSEGLILLTSNGDIVNRILRAENKLEKEYLVAVNHPVTPEFLRGMARGGVPVHG
                     EPTLPCRTGKLGSQGFRIVLTQGLNRQIRLMAAHFGYRVKQLLRVRIGNVKLGHLKPG
                     QWRNLTDAELRGLLPGQADW"
     gene            2582..2791
                     /locus_tag="PK818_00675"
     CDS             2582..2791
                     /locus_tag="PK818_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HQY81280.1"
                     /translation="MSDRQTGTVKWFNDAKGFGFITPESGPDVFVHFRSIQGTGFKSL
                     QEGQKVSFKVVQGQKGLQADEVQAM"
     gene            2944..3747
                     /locus_tag="PK818_00680"
     CDS             2944..3747
                     /locus_tag="PK818_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81281.1"
                     /translation="MRPPRIVPVLALCIALAACDGARAPAADPQPTAPAAPATVVLAD
                     VVETRPDYIVGISYPQSASRHPLLAQALKAYAEAARADLMKAVAGSNGQKPPVPYDLS
                     LQFSGLVDTPEVVAVAADGSTYTGGAHGNPLVARFVWLPQRQSMLEAGQLVGGPAGWQ
                     AISDASREQLMTALSQRLDADELEGADRAAQLRSGSAMIDAGTEPNPRDFALFEPVMN
                     AEGQIRALRFVFPPTQVAPYEEGTRTVEIPARLLLPHVAPDYRPLFQGG"
     gene            complement(3897..6071)
                     /locus_tag="PK818_00685"
     CDS             complement(3897..6071)
                     /locus_tag="PK818_00685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HQY81282.1"
                     /translation="MKHPIAGALALAIATALAGCASTPATTAAAQESPMTGIAYSGPF
                     AAPSTLDLNYPRFDQIKDSDFAPAFDAGMAIQLGEIDAIANNPEPATFQNTIVAMEKT
                     GLLLDRATGVFFNLVGTDKNDARDKLQSGYAPKFSAHRDAITLNPKLFARIKALHDAR
                     ATLGLDAVDQRLLERRYDDFVRGGAALTEAQKARIREINTEMSKLGTQFGQNVLAEVN
                     DSAVVVDTRAELAGMSDEQVAAAGEAAKARGLEGKYVIALLNTTGQPAEAQLQDRALR
                     ERLHKASVARGSRGGKYDNTAIVSQVLKLRAERAKLLGYDTSANFVLADETAGNQDNV
                     NAMLRQLAPAAVANAKREAADLQAMIDREQKAKGQPTFRLEPWDWSYYSEKVRQERYS
                     FDESQLKPYFEMKNVLENGVFFAATKLYGITFKERTDLPKYHPDTWTYDVYDKDGSLL
                     SIFIFDPYARASKRGGAWMNTYVGQSALSGEKPVVANHLNVPKPSAGKPTLLTWDEVT
                     TAFHEFGHALHGFFSDVRYPYFTMNVPRDFVEYPSQVNEMWADWPDVLANYARHYQTG
                     APMPKALLDKVLAASNFNQGFATTEYLGAAMLDQRWHQLPLEQIPDKGGVMPFEAEAL
                     RMDGIDYAPVPPRYKTPYFSHIMGGYAAGYYAYIWSEVLDANTVEFIRRNGGLTRANG
                     DRMRQFVLAPGGEIEAGKLFPNFAGYEAKIGPLLEKRGLTTK"
     gene            6239..6727
                     /locus_tag="PK818_00690"
     CDS             6239..6727
                     /locus_tag="PK818_00690"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HQY81283.1"
                     /translation="MATTAYDFDATALDGSPQPLSDWRGKVLLVVNVASKCGFTPQYA
                     GLEKLWRDFGDKGLVVLGFPCDQFGHQEPGNAEEIRNFCSLTYDVSFPMFARIEVNGA
                     NAHPLWKWLKDEKGGLLGFDAIKWNFTKFLVGRDGRVLKRYAPTDKPESIARDIEQAL
                     AG"
     gene            6724..7128
                     /locus_tag="PK818_00695"
     CDS             6724..7128
                     /locus_tag="PK818_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81284.1"
                     /translation="MTMDVVDGTGYRLELDYEAPLLRASVSGGHDTGLAVSREYWLRI
                     LDHARACGASQLLVLDGMQGEVMGDEDLQRFFDAIEGRVHAGLRMAYVEGRADQIPRI
                     EYAELLARERGYTVRIFNNETDALVWLRHGMR"
     gene            complement(7205..8014)
                     /locus_tag="PK818_00700"
     CDS             complement(7205..8014)
                     /locus_tag="PK818_00700"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007966679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HQY81285.1"
                     /translation="MASPFGTETVLDVRHWTDAYFSFTTTRDDGFRFDNGQFVMIGLE
                     VEQADGARKPLMRAYSIASPNWDEQLEFFSIKVQDGPLTSRLQHIRPGDSILIGRKPT
                     GTLLISDVHPGRNLYLLATGTGLAPWLSIAMDPATYERFEKVIICHGVRSEPDLAYRD
                     YITSALPRHEFLGEQVGRQLLYYPAVSREDFYWHGRLQRGRITDLLDSGRIAADLGLP
                     PLDPALDRAMICGSPQMLADLRAILDRRGFSASPRIGSAGEYVFERAFVEK"
     gene            complement(8119..9291)
                     /locus_tag="PK818_00705"
     CDS             complement(8119..9291)
                     /locus_tag="PK818_00705"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQY81286.1"
                     /translation="MTPMSTLAFAAGLSLALNACAQPAPSPATGTTPAPAAGSGALRV
                     ETVATGLEHPWSVALLPEGGFLVSERPGRLRRISADGQVSAPIAGVPEVFAQGQGGLL
                     DVVLDPGYAGNRRIWLSYAEPGDGDTAGTAVATATLAGDALTDLVVRYRQLPKLEGSA
                     HFGSRIAFDGAGHVFISQGERNRRAMAQDLDVLQGKLVRLHLDGSVPADNPFANASGV
                     RRLVWSYGHRNMQGLAVDPRTGSLWQSEHGPRGGDEINLPQPGRNYGWPVITHGINYS
                     GFDIPEAVGTAAPGMEQPHHVWAKSPALSGMAFLTGHPGNPWNDSLLLGALAGQSLIR
                     LQLDGNRIVAEERLLTDLRARIRDVRVGADGTVYVLTDAGNGQLLRLLPPDARRPR"
     gene            complement(9324..11177)
                     /locus_tag="PK818_00710"
     CDS             complement(9324..11177)
                     /locus_tag="PK818_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005413961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY81287.1"
                     /translation="MPPPLPPAQLNLRQRFSAMRNIAPFLREVWATSRALTLAAIGIR
                     LLRAFLPVATLYIGKLIIDEAVRLVGLDVPRELATAWHAGQLDHLLALLGLEFALAIG
                     SDLLGRMASYVDSVLSEKFTNATSIRLMEHAATLDLEDFEDADLQDKLDRARRQTMGR
                     MNLMGQLFGQAQDVVTVLSFAAGLLAYAPWLLALLAVALVPAFIGESHFNALNYSLNF
                     QWTPERRQLEYLRQMGASVDTAKEVKIFNLNRFFIARFRTLSQKFFEANRRLAGRRAF
                     WGTLLAALGTLGYYVAYAYIAWRTVRGDFSIGDLTFLAGSFRRLRQLLESLLTGFSQV
                     ASQALYLDDLYSFFEIRPQVRSIENALKVPVPVRGGFVFEGVGFRYEGAEQWALRGLS
                     FELHAGEVLALVGENGAGKTTLVKLLARLYDPDEGRILLDGRDLRDYDLDDLRANIGV
                     IFQDFVRYHLTAAENIGVGQIDAMDDRERIERAARKGMADEVIAGLPRGYEQVIGRRF
                     KDGVDLSGGQWQKIAIARAYMRDAQVMILDEPTAALDARSEFEVFERFRELSDGKTAV
                     LISHRFSSVRMADRILVLADGKVEASGTHQQLMSQGGRYAELFELQAAGYR"
     gene            11262..12809
                     /locus_tag="PK818_00715"
     CDS             11262..12809
                     /locus_tag="PK818_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HQY81288.1"
                     /translation="MNSKAPVSIRQEDLVQSVADALQYISYYHPVDYIRSLAAAHERE
                     QSPAAKDAIAQILINSRMCAEGHRPICQDTGIVTVFLEIGMDVRWDDATMGVEDMVHE
                     GVRRAYNHPDNKLRASVLADPAGKRTNTRDNTPGVVNVRVVPGNTVEVIVAAKGGGSE
                     AKSKFAMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPI
                     DITELQARGASNRAEELRLELYDKVNALGIGAQGLGGLTTVLDIKVKDYPTHAANLPV
                     AMIPNCAATRHAHFTLDGSGPVMLDPPSLEDWPQLTYNPQNARRVDLDTVTREDVASW
                     KPGEVLLLNGKLLTGRDAAHKRMVEMLDKGEPLPVDLRGKFIYYVGPVDPVRDEVVGP
                     AGPTTATRMDKFTEQVLAQTGLMGMVGKAERGPAAIEAIRKHRSAYLMAVGGAAYLVS
                     KAIKAAKVVGFADLGMEAIYEFEVRDMPVTVAVDAQGTSVHDTGPREWQARISGRSPG
                     GIPLVVA"
     gene            12806..13201
                     /locus_tag="PK818_00720"
     CDS             12806..13201
                     /locus_tag="PK818_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81289.1"
                     /translation="MSNTRVVNGPGFRVCFDDEPDYLRAYVFDGTDSLAVSTAMWRIL
                     GEECRAAKSTRLLVLEDLMSTVDLPDIAQVVDALIDAGLRDVRMAFVELRDDIQGSEI
                     AESLCLERGMSIRIFSEESEARRWLIYGS"
     gene            complement(13260..13760)
                     /locus_tag="PK818_00725"
     CDS             complement(13260..13760)
                     /locus_tag="PK818_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HQY81290.1"
                     /translation="MDPKTLYYLVAAALVMVGLAGTVLPALPGLPLVFAGMLLAAWVD
                     GFANVPAWVLVLLGLLTLLSLAIDFWATALGAKRVGASRKAVLGAMLGTLAGLFLGPF
                     GLLLGPFAGALGGELLHRRSLGSGDIGNAAKIGFGTWLGILFGVVLKLGLAFTMLGLF
                     ALAWWW"
     gene            complement(13863..14933)
                     /locus_tag="PK818_00730"
     CDS             complement(13863..14933)
                     /locus_tag="PK818_00730"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M35 family metallo-endopeptidase"
                     /protein_id="HQY81291.1"
                     /translation="MKTTLVRAIAGTTILAGAITAATAAAPLTRHNPLRVSMYAAGNG
                     NVEVVLTNTSSKSARVPRYQLPLGELEAKLFSVSRDGEPVAYEGPMIKRGLPSASDFA
                     ILRPGQSVRSVVQLADAYDMRKSGTYTVTLASAMQHASLSDGSMLKTPRGMPLTLQSA
                     PLRMYATGKLPAIKRPGGGTGGTSINGVATVGCSSTQVSGIGNAITAARNYSENAKGY
                     LAGNNQGPRYTTWFGAYTSSRYTTANQHFVAIDSALDKTGDQVKINCGCNQSYYAYVY
                     PTKPYEIFVCRAFWSAPNTGTDSKAGTLVHEMSHFNVVAATDDHVYGQSGAKSLAASD
                     PAKAVDNADSHEYFAENTPSQN"
     gene            complement(15090..15539)
                     /locus_tag="PK818_00735"
     CDS             complement(15090..15539)
                     /locus_tag="PK818_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQY81292.1"
                     /translation="MSNVDPNRRPSAFGRYVFMLLLGLVLGAMAVVMLMRTWEGRKTS
                     QDRLPHALMHVQAWHVDQLKADMAQNRCNATDVLPHLQALRTTANDLEAAFPDLRDDQ
                     RFTAASTAMRAAMDKAMASPPLTCEGLGAAIKQTGETCKGCHQDFRT"
     gene            complement(15597..16337)
                     /gene="phbB"
                     /locus_tag="PK818_00740"
     CDS             complement(15597..16337)
                     /gene="phbB"
                     /locus_tag="PK818_00740"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014090372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HQY81293.1"
                     /translation="MTSRVALVTGGTGGIGTAIVKRLADMGHKVATNYRNEEKARAWQ
                     DKMKAEGYDIAIVKGDVTSPEEAEAMVREVEAALGPVEILVNNAGITRDGTFHRMKAG
                     QWTDVINTNLNSVFNVTRPVIEGMRERKWGRIIQISSINGLKGQYGQANYAAAKAGMH
                     GFTISLARENARNGVTVNTISPGYIATDMVMAVPEEVRAKIVADIPTGRLGTPEEIAY
                     GVGFLADEQASWITGSNLDINGGHHMGW"
     gene            complement(16390..17298)
                     /gene="gluQRS"
                     /locus_tag="PK818_00745"
     CDS             complement(16390..17298)
                     /gene="gluQRS"
                     /locus_tag="PK818_00745"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012480843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HQY81294.1"
                     /translation="MPGIPYRGRFAPSPTGPLHAGSLLAALGSWLLARQAGGEWLLRI
                     EDIDPPREVAGAAAQQLRTLQAFGLRHDGEVAWQSRRGHLYRAALNRLLAQGLAFECH
                     CSRADVAAAGGIHRRCVATAPRPDPAIRLRVADGCEVVFDDAVHGQVSQQVDRDVGDF
                     VLRRTDGLWAYQLAVVVDDAAQGVTDVVRGADLLDSTARQVLLQRALGLPMPRYAHLP
                     LLLGEDGRKLSKSDAALPVDAADPLPSLHMLWTCLGQPPLPAEADAGVPAFLQHAIAG
                     FRLDRVPRRAQAASAAVHNTPATSRD"
     gene            17396..18295
                     /gene="htpX"
                     /locus_tag="PK818_00750"
     CDS             17396..18295
                     /gene="htpX"
                     /locus_tag="PK818_00750"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011051556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HQY81295.1"
                     /translation="MFKRVFLFLATNMAVLALVSIVMSVLGVNPNQFGGLLVMAALFG
                     FGGSIISLLLSKWMAKRSTGAHVIETPRNEAERWLLATVQRQAQAAGIGMPEVAIYDA
                     PEINAFATGANRNNALVAVSTGLLRAMDRDEAEAVLAHEVSHVANGDMVTMALLQGVL
                     NTFVIVLARVVGRVIDGYISGGRDGPGLGYYAIVFVLDMVFGLFASMIAMWFSRHREF
                     RADAGAATLAGRHKMIAALERLAQTHGHSSLPKQVAAFGISGGDGGGLKRLLMSHPPL
                     EERIAALRNAAPQAGGARHGIVA"
     gene            complement(18381..20462)
                     /locus_tag="PK818_00755"
     CDS             complement(18381..20462)
                     /locus_tag="PK818_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQY81296.1"
                     /translation="MAIGKDTALRLMLVDDQVNEAEAIVSSLRNAGIAVRPLRPESLD
                     ELASQLAQQQVDLVLAEYASKNLPFNEVALVVGGCGRDVPLLAVLDGLTDEILDSVQG
                     LGAEGIALRSRPQQLLYAVRTEWTDLDARRSQRQLEAQMRETERRCDALIDSSREPIA
                     YIHEGMHIRANPAYLEMFGYESFEDIEGMSLLDLVAPEHVDAFKQLLKRLSKGEPPPP
                     RHELTARDIEGRDFPAVMEFTAAQYEGEPCQQVIFRRQAQDVDPELARQVEDLKQRDQ
                     GTGLLNRPTFLGLLDTAVAEAAGQGAAHGLLLIEPDHFAQLLEVIGLEDVDQLMAAMG
                     QRLASVAEGGAIARFGEHQLALLSRDSDHMATSELAERVRDAFAEHVLEVGSHSLNTT
                     VSVGGVQIGEKIASTSRVLAKASQGVGAAIAVGGNRVELYDPGAVDRAELERIEAWVA
                     RIRVALEGDGFRLDFQPVIALQGDGSEMYEMFLRLYGTDGEPVAASTYMPIAEEHGLL
                     GLIDRWAVARAIDLIGERQRAGKRTSLLLSLSSEAVQDPTMASFIAANLASAGVPGEQ
                     LVLAVPESKVFTSLREAQEFVGAMAGFGVQIALERFGAGLNSMQLLTHFDPAFLKIDP
                     QFMVDLSKSTDSQQKVRNIASQASSRDIRTIADRVQDATSMTLLFSAGVDFVQGDFLA
                     PASSSMNYDFS"
     gene            complement(20508..21557)
                     /gene="epmB"
                     /locus_tag="PK818_00760"
     CDS             complement(20508..21557)
                     /gene="epmB"
                     /locus_tag="PK818_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011408738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HQY81297.1"
                     /translation="MIPAAPARPHPVPAPNAWRQAWRDAVRDPRELLALLGLGTLAAG
                     ISDAAAAQFPLRVPRGFVARMRRGDPRDPLLRQVLPVLEEDRMMPGFTLDAVGDMQAR
                     RGGGVIHKYAGRALLVATGSCAVHCRYCFRRHYPYAEDTAAAAGWRDAVAVIAGDDAI
                     HEVILSGGDPLSLADHKLAELTDALRAIPHLRRLRIHTRLPVVLPERVDSGLLAWLRG
                     LPWPLVVVLHANHANEFDHAVDTALAALRGTGATLLNQAVLLRGVNDSVAALAELCER
                     GHAAGVLPYYLHQLDRVAGAAHFEVGDHEALALHAGLAERLPGYLVPRLVREIAGQPG
                     KTPLVTPGGSIQAAE"
     gene            21648..22214
                     /gene="efp"
                     /locus_tag="PK818_00765"
     CDS             21648..22214
                     /gene="efp"
                     /locus_tag="PK818_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HQY81298.1"
                     /translation="MATLGMNDVKTGQKILVNNDPCVITETEYVKPGKGQAFTRIKYR
                     SIKSGRVVEMTMKATDDLEQADVVDTDMQFLYADGEYWHFMNQESFEQVQADKAGMGG
                     AEKWLKGEESCVVTLWNGSPIAVQPPNFVELKIVETDPGVRGDTSGGGGKPATLETGA
                     VVRVPLFVGQDEVVKVDTRSGEYVSRVK"
     gene            22219..23559
                     /locus_tag="PK818_00770"
     CDS             22219..23559
                     /locus_tag="PK818_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HQY81299.1"
                     /translation="MTESAPQACDLLIEAGWVVPVVPHGVVLVDHAVVVSGGCVVAVL
                     PIAEARARFVAKQTVARPEAALIPGLVNAHTHNPMTLLRGVADDLPLKVWLQQHIWPI
                     EAAVMGPGFVADGVTLAIAEMLRGGTTCCNENYFFPDVQAATYKRHGFRARVGLPVID
                     FPTAWASSDDEYFERAGEVHDQWRGDALVGTAFAPHAPYTVNDANFERVRMLADQLDV
                     PVHLHLHETAQEVRQSLEKHGQRPIARLDRLGLVNDRLIAVHMTQLTEAEIHLCAERG
                     VSVVHCPESNLKLASGFCPVCALQRAGVNLAIGTDGCASNNDLDMVGETRTAAMLAKA
                     VADDAAGFDAHEALRAATLGGAKAIGFDHLVGSIEPGKQADLACVDLSAIETQPLHNV
                     VSQLVYASARQQVADVWIAGVHKLRQRVLVDIDLDGVLANARQWRERIATVRTP"
     gene            23556..24284
                     /gene="ubiG"
                     /locus_tag="PK818_00775"
     CDS             23556..24284
                     /gene="ubiG"
                     /locus_tag="PK818_00775"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HQY81300.1"
                     /translation="MNAAAGNYSQAELDKFDELAQRWWDPNGPQRALHALNPVRLSYV
                     AERAQLRDAAVLDVGCGGGLLSEGLARAGARVTAIDLAPNLLKVARLHGLESGIKVEY
                     RALAVEALAADAPPAFDAVTCMEMLEHVPDPASVIEACARLLKPGGRLFLSTLNRTPA
                     AFALAIVGAEYVARLLPRGTHRYRDFIRPSELAGWLRDAGLQLEDVSGLMYEPWRNGA
                     RLSSRTDVNYLACARKPGPVEARA"
     gene            24281..24958
                     /gene="gph"
                     /locus_tag="PK818_00780"
     CDS             24281..24958
                     /gene="gph"
                     /locus_tag="PK818_00780"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534888.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HQY81301.1"
                     /translation="MTAHAFPKLVLFDLDGTLLDSAPDMLATVNRMRAARGRAPMPLH
                     ELRPHVSKGSRAMSAAAFPELGGEVPAELIREFLDTYEGELGRHSLLFDGVAELLDAI
                     EAAGSRWGIVTNKPEYLAVQVMPALGWDTRSAILVGGDTLPERKPHPLPLLHSAEQLG
                     VAIGDCAYVGDDQRDIASARAAGMRSVVALWGYRRDDDDPRDWGGDALADRVHDLLDP
                     GLWASLR"
     gene            24955..25641
                     /locus_tag="PK818_00785"
     CDS             24955..25641
                     /locus_tag="PK818_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HQY81302.1"
                     /translation="MSDVDALQAFLAKWRARWPEWAVAEVFVPAAQRERAQAWFALRD
                     ELGDAAWGGQDPLPGDAKLGWWAEELDGWSRGARRHPLGLALQKVQAPWDNLAACLPA
                     LRASRERPANLDGAIFALEPYAEAVAGVAQHLFDSVAPAPARNVVLSLLAERAMRHPD
                     GATPLGDLDVRGWSRGLLAQWPPPGDGTRPGRIHAAVVRGRLQRHAQGKPPALPRLGA
                     LATAWRAARG"
     gene            25706..26629
                     /gene="folE2"
                     /locus_tag="PK818_00790"
     CDS             25706..26629
                     /gene="folE2"
                     /locus_tag="PK818_00790"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HQY81303.1"
                     /translation="MHNRSRHSSLPDVAADAPALARPLDWVGMDAMALPIRLQGEHGQ
                     ALHVPAQVDVAVDLGDAAARGIHMSRLYLQLQESLANEALSAPALRKLLESCIASQQG
                     LSSRARLRLRYDHLLLRPALASGNSGWKNYPVALEAELRDGHLQLSLAFSVEYSSTCP
                     ASAALSRQVNAQRFATDFAGVRPLSNDAVRDWLASERGLAATPHAQRSRAELRVELKP
                     AFDELPLKALVDAVEAALGTPVQTAVKREDEQAFAELNAANLMFCEDAARRVAAALAT
                     DPRVSAWQVRVAHFESLHPHDAVASVSGRNA"
     gene            complement(26753..27619)
                     /locus_tag="PK818_00795"
     CDS             complement(26753..27619)
                     /locus_tag="PK818_00795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQY81304.1"
                     /translation="MRRVLLPLLPCFALAGIAAAQSVAGDASPMVGGEGAVPARATRL
                     PASAQQGAMVLGVTHPAATVEHAGRSLRVTTYGSFAFGISRDQTGEAIVRIRQPASGW
                     IEHRVAITPRDWPVERIDGVPPATVNPPKAIAERIEREQARVVAVRSRDDARTGFAQA
                     FIWPVQGRVSGRFGNQRVYNGTPKSPHSGMDIAAPNGTPVKAPADGVVSFADASLYLT
                     GGTMVLDHGHGISSNFLHLSRIDARVGDVVKQGDVIGAVGATGRATGAHLHWGMNWFD
                     VRIDPLLVLERK"
     gene            complement(27619..>28451)
                     /locus_tag="PK818_00800"
     CDS             complement(27619..>28451)
                     /locus_tag="PK818_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019302579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY81305.1"
                     /translation="VPIITHCEDTPTIDANLALFRAKHGDAIPVECHPDIRSREACIK
                     STRLALELARRHGTQLHVLHISTADELALFEQGPLIRADGSRKPITAETCVHFLRFAR
                     ADYARLGNLIKCNPAIKDEADRQALIDALANDRIDVLATDHAPHTLEEKAKPYAAAPS
                     GLPLVQFALNAALELVHEGRLSTAQVVQKFAHAPAQLFDVANRGFLREGYAADLVMID
                     DTPFTVQRGDVLSRCGWSPFEGTTFRSRIAATWVNGVLAWDGSALVGDPAGQRLRFDR
                     "
BASE COUNT         4265 a         9564 c        10145 g         4477 t
ORIGIN      
        1 ggatcctgat cgtgttcgcg ctgaccacgc cgttctggtc gctgttcgac cagaaggcca
       61 gcacctgggt gatccagggc aaggaaatgg tggtcccgca cgacgcctgg tggtggccga
      121 gctggctggt caaggaagcc gggcagatgc aggcgctgaa cccgttgctg atcatgctgc
      181 tgatcccgtt caacaacatc gtgctgtatc ccgccttgcg gcggatcggc tgggagccga
      241 ccgcgctgcg gcggatgggc ttcggcattg ccttcgcggg catcgcctgg gtcatcgccg
      301 gcctgatcca gttgcagatc gacagcggcg cgcagacctc gctggcgtgg cagacctggc
      361 cctacctgct gctgaccttc ggcgaagtgc tggtgtccgc caccgcgctg gaattcgcct
      421 acagccaggc cacgcagtcg atgaagggcg tgatcatggc gttctggtac ctgaccagca
      481 ccttcggcag cctgtgggtg ctgctcacca acgtcgcggt gcgcaacgag gcggtgaccg
      541 cgcgcatcgc caccaccggc tacagcgaga acgcattcct gatgttcttc ttcgcggtct
      601 tcgccttcgc cgccgcgctg gcgttcgccg cctacgcgcg cacgtatccg atgcaggaca
      661 actaccgggc cgcctgacgg atggatttca cgctcaccct ggccatcgtc gcgatcaccg
      721 tgctggtgtc gtggcgggcg ttcaacgacc gcgccctgct ggaacggctg atcctgtggc
      781 cgccggcggt ggaacggcgc gggcaatacg atcgcctgct gacccacggc ttcatccatg
      841 ccgactggat gcacctgctg ttcaacatga tcaccctgtg gtcgttcggc agcgcggtgg
      901 agcaggtgtt ctcggcgtgg atcaccccgg ccggattcgt gctgttctac ctgtcggcga
      961 tcgtggtcgc gatcctgccg acctggctgc gccatcgccg cgatgccagc taccgcagcc
     1021 tgggggcgtc cggcggggtg gccgcggtgc tgttcgcctc gatcctgttc gatccgtggg
     1081 ccacgctgat cctgttcccg atcccggtgc cgatccccgc gttcgtgttc gcgctgcttt
     1141 acgtggccta cagcatctgg atggaccgcc gcggcggcga caacgtcaac cacagcgcgc
     1201 acctgtgggg cgccgcgtac ggcgtgctgt tcacgctggt gctggagccg cggatcctcg
     1261 gccacttcgc gcgggccgtc ttgaaatcgg gcatcgactg accctggagc caccatgcaa
     1321 tcggacatcc gccatgacgc cgccagccaa cgtttcctga ccgaggtcga tggccacgcc
     1381 gcctatgtcg agtacgaaag gtgcgacggg gtgatggcga tcacccacac catcgtgccc
     1441 gcggcgatcg gcgggcgcgg gatcgccgca gcgctggtgc gggttgcggt cgagcacgca
     1501 cgcgcccagg gcgtgaaggt cgacccgcag tgctcgtacg cggacgcctg gctgcgccgg
     1561 cacccggaat tcgccgagct tcgcgcctga gcccggcgcc gggcgcgtag aatgcgcgcc
     1621 catgcgcctg aaccgacaca tcgccgacag cggcttctgc tcccgccgcg aggccgaccg
     1681 cctggtcgcg gagggccggg tcaccgtcaa cggcctgcgc gcgcggatcg gcgcggagct
     1741 cggcgaaggc gacgaggtcc gcatcgacgg caacacgctg gaggcgcgca ctgccgcaag
     1801 gggccagcgc cgccacgtct acatcgcgct gaacaagccg gtgggggtca ccagcaccac
     1861 cgactccggg gtcaaggaca acatcgtcga ttgcgtcgac cacccgcagc gcatcttccc
     1921 ggtaggccgg ctggacaagg attccgaggg cctgatcctg ctcaccagca atggcgacat
     1981 cgtcaaccgg atcctgcgcg cggagaacaa gctggagaag gaatacctgg tggcggtcaa
     2041 ccacccggtg accccggaat tcctgcgcgg catggcccgc ggcggggtgc cggtccacgg
     2101 cgagccgacc ttgccgtgcc ggaccgggaa gctcggcagc cagggcttcc ggatcgtgct
     2161 gacccagggc ctgaaccggc agatccggtt gatggcggcg catttcggct accgggtgaa
     2221 gcagctgctg cgggtgcgca tcggcaacgt caagcttggc cacctcaagc cggggcagtg
     2281 gcgcaacctg accgatgccg agctgcgcgg cctgctgccc gggcaggccg actggtaggc
     2341 cactgccgcc gccagccacg gcttgcgaaa aataattgca ggccattcac aaatgtcgcg
     2401 gaaagtgcgg tatggtgcgc ccactgtttc aagcgggatc acggtacatc gtgacccgat
     2461 gcggtagatc caagtgatcc gctacatcgt gtgcgttacc ccttgctcga cagtcgcggc
     2521 cgaatccggg aattccggcg ggccgtgtca ccatttcaaa ctgttctagg agtaacaaaa
     2581 catgtcggat cgtcagactg gtaccgtcaa gtggttcaac gacgccaagg gcttcggctt
     2641 catcaccccg gaaagcggcc cggacgtctt cgtccacttc cgctcgatcc agggcaccgg
     2701 cttcaagtcc ctgcaggaag gccagaaggt ctccttcaag gtcgtgcagg gccagaaggg
     2761 cctgcaggcc gacgaagtgc aggcgatgta attcgcctgg cgctcgcgcc tcgaaaagcc
     2821 cggcttcggc cgggtttttt gtttgcggcg ataatggcgt ggctgtcgtc tcacgtagct
     2881 ttcaacgtcc gcccgctagc cttgcccacg aattcgttcc cgatagccac gaccatcgtg
     2941 cacatgcgcc ccccccgcat cgttcccgtc cttgcgctct gcatcgcact cgcggcttgc
     3001 gatggcgccc gcgcgcccgc cgccgacccg cagccaaccg ccccggcggc ccctgccacc
     3061 gtggtcctgg cggacgtggt ggaaacccgc cccgactaca tcgtgggcat cagttacccg
     3121 caatccgcca gccgccatcc gctcctggcg caggcgctga aggcctacgc cgaagctgcc
     3181 cgtgccgacc tgatgaaagc cgttgccggc agcaacgggc agaagccgcc ggtgccgtat
     3241 gacctcagcc tgcagttcag cggcctggtg gacacgccgg aggtggtggc ggtggcagcc
     3301 gacggcagca cctacaccgg cggcgcgcac ggcaacccgc tggtcgcgcg cttcgtctgg
     3361 ctgccgcagc ggcagtcgat gctggaggcg ggccagttgg tcggcggccc cgccggctgg
     3421 caggcgattt ccgatgccag ccgcgaacag ctcatgaccg cgctgtcgca gcggctggac
     3481 gcggacgagc tggagggcgc ggatcgtgcc gcgcagctgc gcagcggcag cgcgatgatc
     3541 gatgccggca ccgagcccaa cccgcgggat ttcgccctgt tcgagccggt gatgaacgcg
     3601 gaagggcaga tccgtgcgct gcgcttcgtg ttcccgccca cccaggtcgc gccttacgag
     3661 gagggcacgc ggacggtgga gatcccggcg cggctcctgc tcccgcacgt cgccccggac
     3721 taccggccgc tgttccaggg cggctgagcc cgggcggcgc gcccgtgccc aggtgcgtgg
     3781 gggcacccac tgcgcccgcg ggcgatcccg gcccaggcat cggcaaccac gcttgaccct
     3841 cgcggaaacg acaacgccgc cttgcggcgg cgtcgcgttg cggacgggtg ctgcgcttac
     3901 ttcgtggtca ggccacgctt ttccagcaac ggtccgatct tcgcctcgta accggcgaaa
     3961 ttcgggaaca gcttgccggc ctcgatctcg ccgcccggcg ccagcacgaa ctggcgcatg
     4021 cggtcgccgt tggcacgggt caggccaccg ttcctcctga tgaactccac ggtgttggcg
     4081 tccagcacct cggaccagat gtaggcgtag tagcccgccg cgtagccgcc catgatgtgg
     4141 ctgaagtagg gcgtcttgta gcgcggcggc accggcgcgt agtcgatgcc gtccatgcgc
     4201 agggcctccg cctcgaacgg catcacgccg cccttgtcgg ggatctgctc cagcggaagc
     4261 tggtgccagc gctggtccag catcgccgcg ccgaggtact cggtggtggc gaagccctgg
     4321 ttgaagttgg aggcggccag caccttgtcc agcaacgcct tcggcatcgg cgcaccggtc
     4381 tggtagtgcc tggcgtagtt ggccagtacg tccggccagt cggcccacat ctcgttgacc
     4441 tgcgacgggt attccacgaa gtcgcgcggc acgttcatcg tgaagtacgg gtagcgcacg
     4501 tcggagaaga agccgtgcag cgcgtggccg aactcgtgga acgcggtggt gacttcatcc
     4561 caggtcagca gggtgggctt gcccgcggac ggcttgggca cgttcaggtg gttggccacc
     4621 accggcttct cgccgctcag cgcggattgc ccgacgtagg tgttcatcca cgcgccgccg
     4681 cgcttggagg cgcgcgcgta cgggtcgaag atgaagatcg acagcaggct gccgtccttg
     4741 tcgtagacgt cgtaggtcca cgtgtccggg tggtacttcg gcaggtcggt gcgttccttg
     4801 aaggtgattc cgtacagctt ggtggcggcg aagaacaccc cgttctccag tacgttcttc
     4861 atctcgaagt acggcttgag ctgcgattcg tcgaagctgt agcgctcctg ccgcaccttc
     4921 tcgctgtagt aggaccagtc ccacggctca agcctgaagg tgggctggcc cttggccttc
     4981 tgctccctgt cgatcatcgc ctgcaggtcg gccgcttcgc gcttcgcgtt ggccaccgcg
     5041 gccggcgcca gctggcgcag catggcgttg acgttgtcct ggttgccggc ggtctcgtcg
     5101 gccagcacga agttggcgga ggtgtcgtaa ccgagcagct tcgcgcgctc tgcgcgcagc
     5161 ttcagcacct gcgacacgat ggcggtgttg tcgtatttgc cgccacggct gccgcgcgcc
     5221 accgaggcct tgtgcaggcg ctcgcgcagg gcgcggtcct gcagctgcgc ctcggccggc
     5281 tggccggtgg tgttgagcag ggcaatcacg tacttgccct ccaggccgcg cgccttggcc
     5341 gcttcgccgg cggcggcgac ctgctcgtcg ctcattccgg ccagttctgc gcgggtgtcg
     5401 accaccaccg ccgagtcgtt cacttcggcc agcacgttct ggccgaactg ggtgccgagc
     5461 ttggacatct cggtgttgat ctcgcggatg cgcgccttct gcgcctcggt cagcgcggcg
     5521 ccgccgcgca cgaagtcatc gtagcggcgt tccagcaggc gttggtcgac ggcgtccagc
     5581 ccgagcgtgg cgcgggcgtc gtgcagggcc ttgatccgcg cgaacagctt cgggttgagg
     5641 gtgatcgcgt cgcggtgcgc ggagaacttc ggcgcgtacc cggactgcag cttgtcgcgc
     5701 gcgtcgttct tgtcggtgcc gaccaggttg aagaacacgc cggtggcgcg gtccagcagc
     5761 aggccggtct tctccatcgc gacgatggtg ttctggaacg tcgccggctc cgggttgttg
     5821 gcgatcgcgt cgatctcgcc cagctggatc gccatgccgg cgtcgaaggc gggggcgaaa
     5881 tcgctgtcct tgatctggtc gaagcgcggg tagttgaggt cgagcgtgct gggtgcggcg
     5941 aacgggccgc tgtaggcgat gccggtcatc ggtgactcct gggccgcggc ggtggtggca
     6001 ggcgtgctgg cgcagccggc gagcgcggtg gcgatggcaa gcgcgagggc gccggcgatg
     6061 gggtgcttca tgggggcctc gtcgggcgtg gaaatccggc ggacagacta gcccagccag
     6121 gcgtggcggg cctgtgacga atggcatggc cggccgccag gcgccgccgc gttggccggg
     6181 gtaggcgcgg gcggcgcgac cgcgcacaat cagcgctccc ccaacgccac aggtcgccat
     6241 ggccaccacc gcctacgatt tcgacgccac cgcactggat ggcagcccgc aaccgctgtc
     6301 cgactggcgc ggcaaggtcc tgctggtcgt caacgtcgcc agcaagtgcg ggttcacccc
     6361 gcaatacgcc gggctggaga agctttggcg cgacttcggc gacaaggggc tggtggtgct
     6421 cggtttcccg tgcgaccagt tcggccacca ggagccgggc aatgccgagg aaatccgcaa
     6481 cttctgctcg ctgacctacg acgtgagctt cccgatgttc gcccggatcg aagtcaacgg
     6541 cgccaatgcg catcccctct ggaagtggct gaaggacgag aagggcgggc tgctgggctt
     6601 cgatgcgatc aagtggaact tcaccaagtt cctggtcggc cgcgatggcc gcgtactcaa
     6661 gcgctacgca ccgaccgaca agccggaatc gatcgcccgc gacatcgagc aggcgctggc
     6721 cggatgacca tggacgtggt cgacggcacg ggctaccggc tcgaactcga ttacgaggcg
     6781 ccgttgctgc gcgccagcgt cagcggcggc cacgacaccg ggctggcggt cagccgcgag
     6841 tactggttgc ggatcctgga ccacgcgcgt gcctgcggtg ccagccagtt gctggtgctg
     6901 gacggcatgc agggcgaggt gatgggcgac gaggacctgc agcgtttctt cgacgcgatc
     6961 gagggcaggg tgcacgcggg gttgcggatg gcctacgtgg aaggccgcgc cgaccagatc
     7021 ccgcgcatcg agtacgccga gctgctggcg cgcgaacgtg gttacaccgt gcgcatcttc
     7081 aacaacgaga ccgatgccct ggtctggctg cggcacggga tgcgctgagg cgggttcctg
     7141 cagcggggcg gggcgtgccc cggtacccat gcagcgccgg ggtgccgggc acgacacggc
     7201 agggtcactt ctcgacgaag gcgcgctcga acacgtattc gccggcgctg ccgatccgcg
     7261 gcgacgcgct gaagccgcgc cggtccagga ttgcccgcaa gtcggccagc atctgcgggc
     7321 tgccgcagat catcgcgcgg tccagcgccg ggtcgagcgg cggcaggccg aggtcggcgg
     7381 cgatgcggcc gctgtccagc aggtcggtga tgcggccgcg ctgcaggcgg ccatgccaat
     7441 agaagtcctc gcggctgacc gccgggtagt acagcagctg cctgccgacc tgctcgccga
     7501 ggaactcgtg gcgcggcagc gcgctggtga tgtagtcgcg gtaggcgagg tccggttcgc
     7561 tgcgcacgcc gtggcagatg atcaccttct cgaagcgctc gtaggtggcc ggatccatcg
     7621 cgatcgacag ccacggcgcc agcccggtcc cggtcgccag cagatacagg ttgcgccccg
     7681 ggtggacgtc gctgatcagc agggtgccgg tcggcttgcg cccgatcagg atcgagtcgc
     7741 cgggcctgat gtgctgcagc cgcgaggtca gcggcccgtc ctgcaccttg atgctgaaga
     7801 actccagttg ttcgtcccag ttcgggctgg cgatggagta cgcgcgcatc agcggcttgc
     7861 gcgcgccgtc cgcctgttcg acttccagcc cgatcatcac gaactggccg ttgtcgaagc
     7921 ggaaaccgtc gtcgcgggtg gtggtgaagc tgaagtacgc gtccgtccag tgtcggacgt
     7981 cgagcaccgt ttcggtgccg aagggagagg ccatggggtg ccgtggggat acgtgatccc
     8041 gcccatttta gcggagcgcg accgcccggg ccggtgggcg tgcacgggcg tggcgcgcgg
     8101 ctgctcccgg cgcaccgctc atcgcggccg gcgcgcgtcc ggcgggagca accgcagcag
     8161 ctggccgttg ccggcatcgg tgagcacgta cacggtgccg tcggcgccga cgcggacatc
     8221 gcggatgcgc gcgcgcaggt cggtgagcag gcgttcctcg gcgacgatgc ggttgccgtc
     8281 cagctgcagc cggatcaggc tctggccggc caacgcgccc agcagcaggc tgtcgttcca
     8341 cgggttgccg ggatggccgg tgaggaacgc catgccggac agcgcgggcg acttcgccca
     8401 cacgtggtgc ggctgttcca tgccgggagc ggcggtgccc accgcttccg ggatgtcgaa
     8461 gccggaatag ttgatgccgt gggtgatcac cggccagccg tagttccggc ccggctgcgg
     8521 caggttgatc tcgtcgccgc cgcgcgggcc gtgctcgctt tgccagaggc tgccggtgcg
     8581 cggatcgacc gccagtccct gcatgttgcg gtggccgtag ctccagacca gcctgcgcac
     8641 gccgcttgcg ttcgcgaacg ggttgtcggc gggcacgctg ccgtccaggt gcaggcgcac
     8701 cagcttgccc tgcaggacgt ccaggtcctg cgccatcgcc cgccggttgc gttcgccctg
     8761 gctgatgaac acatgcccgg cgccatcgaa ggcgatgcgc gagccgaaat gcgcactgcc
     8821 ctccagcttg ggcagctgcc ggtagcgcac gaccaggtcg gtgagcgcat cgccggcaag
     8881 ggtggcggtg gcaaccgcgg tgccggcggt gtcgccatcg ccgggttcgg cgtagctcag
     8941 ccagatgcgc cggttgccgg cgtagcccgg gtccagcacc acgtccagca agccgccctg
     9001 gccctgcgcg aatacctccg gcaccccggc gatcggcgcg gatacctggc catcggcgct
     9061 gatccggcgc aaccggccgg ggcgttcgct gaccaggaag ccgccttccg gcaacagggc
     9121 caccgaccac gggtgttcca gcccggtcgc gacggtttcg acccgaagtg cgccggatcc
     9181 ggccgcgggt gccggcgtgg tcccggtggc gggcgatggc gccggttgcg cacaggcatt
     9241 cagggccagc gacaggccgg cggcgaaggc gagcgtggac atcggcgtca tgacgatcct
     9301 ccgggattcc tgtggagccg cgctcatcgg tagcccgccg cctgcaattc gaacagttcg
     9361 gcgtagcgcc cgccctgcga catcagctgc tggtgggtgc cgctggcctc gaccttgccg
     9421 tcggccagca ccaggatgcg gtcggccatg cgcacgctgc tgaagcggtg gctgatcagc
     9481 accgcggtct tgccgtccga cagttccctg aagcgttcga acacctcgaa ttcgctgcgc
     9541 gcatccagcg ccgcggtggg ttcgtcgagg atcatcacct gcgcatcgcg catgtaggcg
     9601 cgggcgatcg cgatcttctg ccactgcccg ccggacaggt ccacgccatc cttgaagcgg
     9661 cggccgatca cctgctcgta gccgcgcggc aggccggcga tgacctcgtc ggccatgccc
     9721 ttgcgcgcgg cgcgctcgat ccgctcgcgg tcgtccatcg catcgatctg gccaaccccg
     9781 atgttctccg ccgcggtcag gtggtagcgc acgaagtcct ggaagatcac cccgatgttg
     9841 gcgcgcaggt cgtcgaggtc gtaatcccgc aggtcgcggc catcgagcag gatgcggccc
     9901 tcgtcggggt cgtacagccg cgccagcagc ttgaccaggg tggtcttgcc ggcgccgttc
     9961 tcgccgacca gcgccagcac ttcgccggcg tgcagttcga agctgaggcc gcgcagcgcc
    10021 cactgctccg cgccttcgta acggaagccg acgccttcga acacgaagcc gccgcggacc
    10081 ggcaccggca ccttcaaggc attctcgatg ctgcggacct gcggccggat ctcgaagaac
    10141 gagtacaggt cgtccaggta cagggcctgg cttgcgacct gcgagaaccc ggtcagcagg
    10201 ctctccagca actggcgcag gcggcggaag ctgccggcga ggaaggtcag gtcgccgatc
    10261 gagaaatcgc cgcgcaccgt gcgccaggcg atgtaggcat aggccacgta gtaaccgagg
    10321 gtgccgagcg cggcgagcag ggtgccccag aacgcgcgcc gccctgcaag cctgcggttg
    10381 gcctcgaaga atttctgcga cagcgtgcgg aagcgggcga tgaagaagcg gttgaggttg
    10441 aagatcttga cctctttcgc ggtgtccacg ctggcgccca tctgccgcag gtattccagc
    10501 tggcggcgct ccggcgtcca ctggaaattg agcgaatagt tcagcgcatt gaagtgcgat
    10561 tcgccgatga acgccggcac cagcgccacc gccagcagcg ccagcagcca cggcgcgtag
    10621 gccagcaggc cggcggcgaa gctgagcacg gtgaccacgt cctgggcctg gccgaacagc
    10681 tggcccatca ggttcatccg gcccatggtc tgccgccgcg cgcggtccag cttgtcctgc
    10741 aggtcggcgt cctcgaagtc ctcaaggtcg agggtggccg cgtgctccat caggcggatg
    10801 ctggtggcgt tggtgaattt ctccgacaac acggaatcga cgtagctcgc catccgcccg
    10861 agcaggtcgg agccgatcgc cagcgcgaat tccaggccga gcagcgcgag caggtggtcc
    10921 agctgtcccg cgtgccaggc ggtggcgagc tcccgcggca cgtccaggcc gaccaggcgc
    10981 accgcctcgt cgatgatcag cttgccgatg tacagggtgg cgaccggcag gaacgcccgc
    11041 agcaggcgga tcccgatcgc ggcgagggtc agtgccctgc tggtggccca gacctcgcgc
    11101 aggaacggcg cgatgttgcg catcgcgctg aagcgctggc gcaggttgag ctgggctggc
    11161 ggcaggggtg ggggcatcgg tccagtctgc ggaaggcggc gtgcggccca ggtggacgca
    11221 gcctgggcct aaaatacccg cttcgtcccg cgccgatcgc catgaacagc aaagccccgg
    11281 tttccatccg ccaggaagac ctggtgcagt ccgtcgccga cgccctgcag tacatctcgt
    11341 actaccaccc ggtcgactac atcaggagcc tcgccgcggc ccatgagcgc gagcaatcgc
    11401 cggccgcgaa ggacgccatc gcgcagatcc tgatcaattc gcggatgtgc gccgagggcc
    11461 accgcccgat ctgccaggac accggcatcg tcaccgtgtt cctcgagatc ggcatggacg
    11521 tgcgctggga cgacgccacc atgggcgtgg aggacatggt ccatgaaggc gtgcgccgcg
    11581 cctacaacca cccggacaac aagctgcgcg ccagcgtgct ggccgatccg gcgggcaagc
    11641 gcacgaacac cagggacaac acgccgggcg tggtcaacgt cagggtggtg ccgggcaaca
    11701 cggtcgaggt gatcgtcgcc gccaagggcg gtggttcgga agccaagtcg aagttcgcga
    11761 tgctcaatcc gtccgattcc atcgtcgact gggtgctgaa gacggtgccg acgatgggcg
    11821 ccggctggtg cccgccgggc atgctcggca tcggcatcgg tggcaccgcc gagaaggcga
    11881 tgctgctggc caaggaagca ctgatggagc cgatcgacat caccgagctg caggcgcgcg
    11941 gcgcgtcgaa ccgcgccgag gaactgcgcc tggagctgta cgacaaggtc aatgcgctcg
    12001 gcatcggcgc gcaggggctt ggcggcctga ccaccgtgct cgacatcaag gtcaaggact
    12061 acccgaccca cgccgccaac ctgccggtgg cgatgattcc caattgcgcg gccacccgcc
    12121 acgcgcactt caccctcgat ggcagcggcc cggtgatgct ggatccgccg tcgctcgaag
    12181 actggccgca gctcacctac aacccgcaga acgcgcgccg ggtggacctc gacacggtca
    12241 cccgggaaga cgtcgccagc tggaagccgg gcgaagtgct gctgctcaac ggcaagctgc
    12301 tgaccggccg cgatgccgcg cacaagcgca tggtcgagat gctcgacaag ggcgaaccgc
    12361 tgccggtcga cctgcgcggc aagttcatct actacgtggg cccggtcgat ccggtgcgcg
    12421 atgaggtcgt cggccccgcc ggcccgacca ccgccacccg catggacaag ttcaccgagc
    12481 aggtgctggc gcagaccggc ttgatgggca tggtcggcaa ggccgagcgc ggcccggccg
    12541 cgatcgaggc gatccgcaag caccgctccg cctacctgat ggcggtcggc ggcgcggcct
    12601 acctggtttc gaaggcgatc aaggccgcga aggtggtcgg tttcgccgac ctcggcatgg
    12661 aggcgatcta cgaattcgag gtgcgcgaca tgccggtgac ggtggccgtg gatgcgcaag
    12721 ggacctcggt gcacgacacc gggccgaggg aatggcaggc gcggatttct ggccgctcgc
    12781 ctggcgggat cccgttggtc gtggcatgag caacacccgc gtcgtcaacg gtccggggtt
    12841 ccgcgtctgc ttcgacgacg aacccgacta cctgcgcgcc tacgtgttcg atggcaccga
    12901 ttcgcttgcg gtgtcgactg cgatgtggcg gatcctgggc gaggaatgcc gcgccgccaa
    12961 gagcacgcgg ttgctggtgc tggaggacct gatgtcgacg gtggatctcc cggacatcgc
    13021 ccaggtggtg gacgcgctga tcgacgccgg cctgcgcgac gtgcggatgg ccttcgtcga
    13081 gctgcgagac gatatccagg gcagcgagat cgccgagtcg ctgtgcctgg agcgcggcat
    13141 gagcatccgc atcttctccg aggaaagcga ggcgcggcgc tggctgatct acggcagctg
    13201 acgcggcagg cgcgacggcg cagcctgctt cggcgcgggc caggcacgcg ttggaggcct
    13261 caccaccacc aggccagcgc gaacaggccg agcatcgtga acgccagacc gagcttgagc
    13321 accaccccga acaggatccc gagccaggtg ccgaagccga tcttcgcggc attcccgatg
    13381 tcgccgctgc ccaggctgcg ccggtgcagc agttcgccgc caagggcacc ggcaaacggc
    13441 ccgagcaaca ggccgaacgg cccgaggaac aggccggcca gggttcccag catcgccccg
    13501 agcaccgcct tgcggctggc gccgacgcgc ttggcgccga gcgcggtcgc ccagaagtcg
    13561 atcgccagcg agagcagggt gagcagcccg agcaggacca gcacccaggc gggaacgttg
    13621 gcgaacccgt ccacccaggc cgcaagcagc atcccggcga acaccagcgg caggcccggc
    13681 aaggcgggca gcacggtgcc ggcgaggccg accatgacca gggcggcggc aaccaggtag
    13741 tagagcgtct tcgggtccat gcgcgggccg gtggcaacgg gagccgcatt ctgcccggac
    13801 ttccaccgca aacacgaacg gcccgcagtg cgggccgtcc gggtggtgcg atgcggcgtc
    13861 cgtcagttct gcgacggggt gttctcggcg aagtactcgt ggctgtcggc attgtcgacc
    13921 gccttggccg gatccgaggc cgccaggctc ttggcgccgg actggccgta gacatggtcg
    13981 tcggtcgccg ccaccacgtt gaagtggctc atctcgtgga ccagggtgcc cgcctttgaa
    14041 tcggtgccgg tgttcggcgc cgaccagaac gcacggcaga cgaagatctc gtacggcttg
    14101 gtcggataga cgtaggcgta gtagctctgg ttgcagccgc agttgatctt gacctggtcg
    14161 ccggtcttgt ccagcgcgct gtcgatggcg acgaagtgct ggttggcggt ggtgtagcgg
    14221 ctcgaggtgt aggcgccgaa ccaggtggtg tagcgcgggc cctggttgtt gccggcgagg
    14281 tagcccttgg cgttttcgga atagttgcgg gccgcggtga tcgcattgcc gatcccgctg
    14341 acctgggtgg acgagcaacc gaccgtggcg acgccgttga tgctggtgcc gccggtgccg
    14401 ccaccggggc gcttgatcgc cggcagcttg ccggtggcgt acatgcgcaa cggggcgctc
    14461 tgcagggtca gcggcatgcc gcgcggggtc ttcagcatgc tgccatcgga cagcgaggcg
    14521 tgctgcatgg cggaggccag ggtgacggtg taggtgccgc tcttgcgcat gtcgtaggcg
    14581 tcggccagct ggaccacgct gcgaacgctc tggccgggcc gcaggatcgc aaaatcggac
    14641 gcggagggca ggccgcgctt gatcatcggg ccctcgtagg cgaccggctc gccgtcgcgg
    14701 ctcacgctga acagcttggc ttccagttcg cccagcggca gctggtacct cggcacccgt
    14761 gcgctcttgc tgctggtatt ggtgagcacg acttcgacgt tgccgttgcc ggcggcgtac
    14821 atgctgacgc gcagcgggtt gtggcgggtc agcggcgccg ctgcggtggc agcagtgatc
    14881 gcgccggcaa ggatggtggt gccggcgatg gcacggacga gcgtggtttt cattcagttc
    14941 cccggttgaa tggtcgatgg caggcccgcc tgatgcggac gagcgacaat aaccgtgatc
    15001 gccgtcacgg gcattttgca ccgcagcatg cccgcgcccg aggcgtgcgc gcggtcgtca
    15061 gggcggccag gtccggggtt tgccgtggct caggtgcgga agtcctggtg gcagcccttg
    15121 caggtttcac cggtctgctt gatcgccgca ccaaggccct cgcaggtcag cggcgggctg
    15181 gccatggcct tgtccatcgc cgcgcgcatg gcggtgctgg cggcagtaaa gcgctggtcg
    15241 tcgcgcaggt cggggaacgc ggcttccagg tcgttggcgg tggtccgcag cgcctgcagg
    15301 tgcggcagta cgtcggtcgc gttgcagcgg ttctgcgcca tgtccgcctt gagctggtcg
    15361 acgtgccatg cctgcacgtg catcagcgca tgcgggagcc ggtcctgcga cgtcttgcgc
    15421 ccttcccagg tgcgcatcag catcaccacc gccatcgccc ccagcaccag gccaagcagc
    15481 agcatgaaga catagcggcc gaaggcggac gggcggcggt tcgggtctac gttggacatg
    15541 gggtctccgt gttttcgttg tctggggcgg atgccggggg cgcggctgcg taaccgctac
    15601 cagcccatgt ggtggccgcc gttgatgtcg aggttgctgc cggtgatcca gctggcctgc
    15661 tcgtcggcca ggaagccgac gccataggcg atctcctccg gggtgccgag gcgaccggtg
    15721 ggaatgtcgg ccacgatctt ggcgcgcact tcctccggca ccgccatcac catgtcggtg
    15781 gcgatgtagc cgggcgagat ggtgttgacg gtcaccccgt tgcgggcgtt ttcgcgcgcc
    15841 agcgagatgg tgaaaccgtg catgccggcc ttggcggcgg cgtagttggc ctggccgtac
    15901 tggcccttga ggccgttgat cgagctgatc tggatgatcc ggccccactt gcgttcgcgc
    15961 atgccctcga tcaccgggcg ggtgacgttg aacaccgaat tgaggttggt gttgatcacg
    16021 tcggtccact gcccggcctt catccggtgg aaggtgccgt cgcgggtgat gccggcgttg
    16081 ttgaccagga tctcgaccgg gccgagcgcg gcttcgactt cgcgcaccat cgcctcggct
    16141 tcctccggcg aggtcacgtc gcccttgacg atggcgatgt cgtagccctc ggccttcatc
    16201 ttgtcctgcc aggcgcgtgc cttttcctcg ttgcggtagt tggtggcgac cttgtggccc
    16261 atgtctgcca accgcttgac gatggcggtg ccgatgccgc cggtgccgcc ggtcacgagc
    16321 gcaactctgg aagtcatgcg tatctcctgg gggtgatggg taagtggcac ggatgcaggt
    16381 tgcccgattc tagtcacgcg atgttgcggg tgtgttgtgc actgcggcgg atgcggcctg
    16441 cgcgcgccgc gggacccggt ccaggcggaa cccggcgatg gcatgttgca ggaatgcggg
    16501 caccccggca tccgcctcgg cgggcagcgg cggttgcccg aggcaggtcc agagcatgtg
    16561 cagggagggc agcggatcgg cggcatcgac cggcagcgcg gcatccgatt tcgacagctt
    16621 cctgccatcc tcgccgagca gcagcggcag gtgggcgtag cgcggcatcg gcaggccgag
    16681 cgcgcgttgc agcaggacct ggcgggcggt ggaatcgagc aggtcggcgc cacgcacgac
    16741 atcggtgaca ccctgcgcgg cgtcgtccac caccaccgca agctggtagg cccacaggcc
    16801 atccgttcgc cgcaggacga agtcgccgac gtcgcggtcg acctgctggc tgacctggcc
    16861 gtgcacggcg tcgtcgaaca cgacctcgca gccatccgcc acccgcagcc ggatcgccgg
    16921 gtcggggcgc ggcgcggtgg ccacgcagcg gcgatggatg ccgccggcgg cggccacgtc
    16981 ggcgcggctg cagtggcatt cgaaggccag cccctgcgcc agcaaccggt tcagcgcagc
    17041 gcgatacagg tgcccgcgcc ggctctgcca tgcgacttcg ccgtcgtggc gcaggccgaa
    17101 cgcctgcagc gtgcgcaact gctgggcggc ggcgccggcc acctcgcgcg gggggtcgat
    17161 gtcctcgatc cgcagcagcc actcgccgcc ggcctggcgg gccagcagcc aactgccgag
    17221 tgccgccagc agcgaaccgg catgcagcgg tccggtgggc gagggggcga agcggccgcg
    17281 gtaggggatg cctggcatcg ggctgtgtcg acagggattt cagattgaat ttaggttcga
    17341 ccggccacac ttctaacgag acccgcgcat ccgcgcggaa cgaatgggat tatccatgtt
    17401 caagcgcgtc ttcctgttcc tggccaccaa catggccgtg ctcgccctgg tcagcatcgt
    17461 gatgtcggtg ctgggggtga accccaacca gttcggcggc ctgctggtca tggccgcatt
    17521 gttcggcttc ggcggctcga tcatctcgct gctgctgtcg aagtggatgg ccaagcgcag
    17581 caccggtgcg cacgtgatcg agaccccgcg caacgaggcc gagcgctggc tgctggccac
    17641 ggtccagcgg caggcgcagg cagcaggcat cggcatgccg gaagtggcga tctacgacgc
    17701 tcccgaaatc aacgccttcg ccaccggcgc caaccgcaac aacgcgctgg tggcggtctc
    17761 caccggcctg ctgcgggcga tggaccgcga cgaggcggag gcggtgctgg cgcacgaggt
    17821 cagccacgtc gccaacggcg acatggtgac catggcgctg ttgcagggcg tcctgaacac
    17881 gttcgtgatc gtgctggcgc gggtggtcgg gcgggtgatc gacggctaca tcagtggtgg
    17941 ccgcgatggc cccgggctcg gctactacgc gatcgtgttc gtgctggaca tggtgttcgg
    18001 cctgttcgcc tcgatgatcg cgatgtggtt ctcccgccat cgcgagttcc gcgccgatgc
    18061 cggcgccgcg accctcgccg gcaggcacaa gatgatcgcc gcgctggagc ggctggcgca
    18121 gacccatggc cacagcagcc tgccgaagca ggtggcggcc ttcggcatca gcggcggcga
    18181 cggcggtggc ctcaagcgcc tgctgatgag ccatccgccg ctggaggagc ggatcgccgc
    18241 gctgcgcaac gcggcaccgc aagcaggcgg cgcgcgccac ggcatcgttg cctgacccac
    18301 cgtaaccaac gccggtaaac gaaaagcccg ccggaccggc gggctttttc attccagcaa
    18361 caggccagga agtggcccgc tcagctgaag tcgtagttca tgctcgagct ggccggggcc
    18421 aggaagtcgc cctgcacgaa gtcgaccccg gcggagaaca gcagggtcat gctggtcgcg
    18481 tcctggaccc ggtccgcgat ggtgcggatg tcgcggctgc tggcctggct tgcgatgttg
    18541 cggaccttct gctgcgaatc cgtgctcttc gagaggtcga ccatgaactg cgggtcgatc
    18601 ttcaggaagg ccgggtcgaa gtgggtcagc agctgcatcg aattgaggcc ggcaccgaag
    18661 cgctccagtg cgatctgcac gccgaagccg gccatcgccc cgacgaattc ctgcgcctcg
    18721 cgcaggctgg tgaagacctt ggactccggc accgccagca ccagctgttc gcccggaacg
    18781 ccggccgaag cgaggttcgc ggcgatgaag ctggccatgg tcgggtcctg caccgcctcg
    18841 ctggacagcg acagcagcag gctggtgcgc ttgccggcgc gctggcgctc gccgatcagg
    18901 tcgatcgcgc gggcgaccgc ccagcggtcg atcaggccca gcaggccgtg ttcctcggcg
    18961 atcggcatgt aggtgctggc cgcgaccggc tcgccgtcgg tgccatacag gcgcaggaac
    19021 atttcgtaca tctcgctgcc gtcgccctgc agcgcgatca ccggctggaa atcgaggcgg
    19081 aaaccgtcgc cctccagcgc gaccctgatc cgggccaccc aggcctcgat ccgctccagt
    19141 tcggcgcggt ccaccgcgcc ggggtcgtac agctcgaccc ggttgccgcc gaccgcgatc
    19201 gccgcgccca cgccctggct ggccttcgcc agcacccggc tggtgctggc gatcttctcg
    19261 ccgatttgca cgccgccgac gctgaccgtg gtgttgagcg aatggctgcc gacttccagc
    19321 acatgctcgg cgaatgcgtc gcgcacgcgc tcggccagtt cgctggtggc catgtggtcg
    19381 ctgtcgcgcg acagcagggc cagctggtgc tcgccgaagc gcgcgatcgc accgccctcg
    19441 gcgacgctgg cgaggcgctg gcccatcgcc gccatcagct ggtcgacgtc ttccaggccg
    19501 atcacctcca gcagctgggc gaagtggtcc ggctcgatca gcagcaggcc gtgggccgcg
    19561 ccctggccag cggcttcggc aacggcggta tccagcaggc cgaggaaggt ggggcggttg
    19621 agcaggccgg tgccctggtc gcgttgcttg aggtcttcga cctggcgcgc cagttcgggg
    19681 tccacgtcct gggcctggcg gcggaagatc acctgctggc agggctcgcc ctcgtactgg
    19741 gcggcggtga attccatcac cgccgggaag tcgcggcctt cgatgtcgcg cgcggtcagt
    19801 tcgtggcgcg gcggtggcgg ctcgcccttc gacaggcgct tcagcagttg cttgaaggca
    19861 tccacgtgtt ccggcgcgac caggtcgagc agcgacatgc cctcgatgtc ttcgaaggat
    19921 tcatagccga acatctccag gtaggccggg tttgcgcgga tgtgcatgcc ttcatggatg
    19981 taggcgatcg gctcgcgcga ggaatcgatc agcgcatcgc agcggcgctc ggtctcgcgc
    20041 atctgcgctt ccagctggcg ctgcgagcgg cgggcatcga ggtccgtcca ctcggtgcgc
    20101 accgcgtaca gcagctgctg cgggcggctg cgcagggcga tgccctccgc gccgaggccc
    20161 tgcacggaat ccaggatttc gtcggtcagg ccgtcgagga cggccagcag cggcacgtcg
    20221 cgcccgcaac caccgaccac cagcgccact tcgttgaacg gcaggttctt cgatgcgtat
    20281 tcggccagca ccaggtcgac ctgctgctgg gcaagctggc tggccagctc gtccagcgac
    20341 tccggccgca gcgggcgtac cgcgatgccg gcattgcgca ggctgctgac gatcgcctcg
    20401 gcctcgttga cctggtcgtc taccagcatc agtcgcagtg cggtgtcctt accgatggcc
    20461 atgcgtgggt ctttcgctcg aatccggggg gatggcggga ggctacatca ttccgcggcc
    20521 tggatgctgc cgccaggggt cacaagcggg gtcttgccgg gctggccggc aatctcgcgc
    20581 acgagccgcg gcaccaggta cccgggcagg cgctcggcca gtccggcatg cagggccagc
    20641 gcctcgtggt cgccgacctc gaaatgggcg gcgccggcga cgcggtcgag ctggtgcagg
    20701 tagtagggca ggacgccggc ggcgtgcccg cgctcgcaaa gctcggccag cgccgcgacc
    20761 gagtcgttca ccccgcgcag cagcacggcc tggttgagca gggtggcgcc ggtgccgcgc
    20821 agggccgcca gcgcggtgtc caccgcgtgg tcgaactcgt tggcatggtt ggcgtgcagt
    20881 accaccacca gcggccacgg cagtcctcgc aaccacgcca gcaggccgga atccacgcgt
    20941 tccggcagca ccaccggcag ccgggtgtgg atgcgcaggc ggcgcaggtg ggggatcgcg
    21001 cgcagggcgt cggtcagctc tgccagcttg tggtcggcca gcgacagcgg gtcgccgccg
    21061 gacaggatga cctcgtggat cgcgtcgtcg cccgcgatca ccgccaccgc gtcgcgccag
    21121 ccggccgccg ccgcggtgtc ctcggcatac ggatagtggc ggcggaaaca gtagcggcag
    21181 tgcaccgcgc agctgccggt ggcgaccagc agggcgcgcc cggcgtactt gtggatgacc
    21241 ccgccgccgc gccgggcctg catgtcgccg actgcatcga gggtgaagcc gggcatcatg
    21301 cgatcctcct cgaggaccgg cagcacctgc cgcaacagcg ggtcgcgcgg gtcgccacgg
    21361 cgcatgcggg cgacgaaccc gcgcggcacc cgcagcggga actgcgccgc cgcggcatcg
    21421 gatatgccgg cggccagcgt gcccaggccc agcagcgcca gcagctcgcg cgggtcgcgc
    21481 acggcatcgc gccaggcctg gcgccaggcg ttcggcgcgg gcacgggatg cgggcgtgcg
    21541 ggggcagcgg gtatcattgc ggtttcgatc cgatcggtcc cggcgccacg ccgggatccc
    21601 agaacccaca ttttaggccg cccggcgggt ggcgcgaagg agcacccatg gccaccctgg
    21661 gcatgaacga cgtcaagacc ggccagaaga tcctggtcaa caacgatccc tgcgtgatca
    21721 ccgagaccga atacgtcaag cccggcaagg gccaggcctt cacccgcatc aagtaccgca
    21781 gcatcaagtc cgggcgggtg gtggaaatga ccatgaaggc caccgacgac ctcgaacagg
    21841 ccgacgtggt cgataccgac atgcagttcc tgtacgccga tggcgagtac tggcacttca
    21901 tgaaccagga gtcgttcgag caggtgcagg ccgacaaggc cggcatgggc ggcgccgaga
    21961 agtggctgaa gggcgaggaa tcgtgcgtgg tgacgctgtg gaacggcagc ccgatcgcgg
    22021 tgcagccgcc gaacttcgtc gaactcaaga tcgtcgagac cgacccgggc gtgcgcggcg
    22081 acacctcggg cggcggcggc aagccggcca cccttgagac cggtgcggtg gtgcgagtgc
    22141 cgctgttcgt cggccaggac gaggtggtca aggtcgatac ccgcagcggc gaatacgtca
    22201 gccgcgtgaa gtaagccgat gaccgaatcc gccccgcagg cctgcgacct cctgatcgaa
    22261 gccggctggg tggtgccggt ggtcccgcac ggcgtggtcc tggtcgacca cgccgtcgtc
    22321 gtttccggcg gatgcgtcgt cgccgtgctg ccgatcgccg aggcgcgcgc tcgcttcgtg
    22381 gcgaagcaga cggtcgcgcg ccccgaggcc gcgctgattc ccggcctggt caacgcccac
    22441 acccacaacc cgatgaccct gctgcgcggg gtcgccgacg acctgccgct gaaggtctgg
    22501 ctgcagcagc acatctggcc gatcgaggcg gcggtgatgg ggccgggctt cgtcgccgac
    22561 ggcgtgaccc tggcgatcgc cgagatgctg cgcggcggca ccacctgctg caacgagaac
    22621 tacttcttcc ccgacgtgca ggccgcgacc tacaagcgcc acggcttccg cgcgcgggtc
    22681 gggctgccgg tgatcgactt cccgaccgcc tgggcgagca gcgacgacga atacttcgag
    22741 cgcgccggcg aggtccacga ccagtggcgc ggcgacgccc tggtcggcac cgcgttcgcg
    22801 ccgcatgcgc cgtacacggt gaacgatgcc aacttcgagc gcgtgcgcat gctggccgac
    22861 caactggacg tgccggtgca cctgcacctg cacgagaccg cgcaggaagt ccggcagtcg
    22921 ctggaaaagc acggccagcg cccgatcgcg cggctggacc ggctggggct ggtcaacgac
    22981 cgcctgatcg cggtgcacat gacccagctg accgaggccg agatccacct gtgcgccgaa
    23041 cgcggggtca gcgtggtgca ctgcccggaa tccaacctca agctggcgtc cggcttctgc
    23101 ccggtctgcg cgctgcaacg cgcgggcgtc aacctcgcca tcggcaccga tggctgcgcg
    23161 tccaacaacg acctcgacat ggtcggcgag acccgcaccg cggcgatgct ggcgaaggcg
    23221 gtggccgatg atgccgccgg cttcgacgcg cacgaggccc tgcgcgcggc gaccctgggc
    23281 ggggcgaagg cgatcggctt cgaccacctc gtcggcagca tcgagccggg caagcaggcc
    23341 gacctcgcct gcgtggacct gtccgcgatc gagacccagc ccctgcacaa cgtggtctcg
    23401 cagctggtct acgcgagcgc ccgccagcag gtcgccgatg tctggatcgc gggcgtgcac
    23461 aagctgcgcc agcgggtact ggtcgacatc gacctcgatg gcgtgctcgc caacgccagg
    23521 caatggcgcg aacggatcgc aacggtgcgc acaccatgaa tgcagcagct ggcaattact
    23581 cgcaggccga gctcgacaag ttcgacgaac tggcccagcg ctggtgggat cccaacgggc
    23641 cgcagcgggc gctgcatgcg ctcaaccccg tgcgcctgtc ctatgtggcc gaacgcgcgc
    23701 agttgcgcga tgccgcggtg ctggacgtgg gctgcggcgg cggcctgctc agcgaggggc
    23761 tggcacgggc cggggcaagg gtcaccgcca tcgacctggc cccgaacctg ctgaaggtcg
    23821 cgcgcctgca tgggctcgag tccgggatca aggtcgagta ccgcgcgctg gcggtcgagg
    23881 cgctggccgc ggacgcgcca ccggcgttcg acgcggtcac ctgcatggaa atgctggagc
    23941 acgtgccgga cccggcatcc gtgatcgaag cctgcgcgcg gctgctgaaa cccggtggcc
    24001 gcctgttcct gtccaccctg aaccgcaccc cggccgcgtt cgcgctggcg atcgtcggcg
    24061 ccgagtacgt cgcccgcctg ctgccgcgcg gcacccaccg ctaccgcgac ttcatccggc
    24121 catcggaact ggccgggtgg ctgcgcgacg ccggcctgca gctggaggac gtcagcggcc
    24181 tgatgtacga gccgtggcgc aacggcgcgc ggctgtcgtc gcgcaccgac gtgaattacc
    24241 tcgcctgcgc gcgcaagccc ggcccggtcg aagcacgcgc atgaccgcgc atgcgttccc
    24301 gaaactggtg ctgttcgacc tcgacggcac cctgctggac agcgcgccgg acatgctggc
    24361 cacggtcaac cgcatgcgcg cggcgcgcgg gcgcgcaccg atgccgctgc acgaactgcg
    24421 cccgcacgtg tcgaagggtt cgcgggcgat gagcgcggcc gccttccccg aactgggcgg
    24481 cgaggtgccg gcggagctga tccgagaatt cctcgacacc tacgaaggcg aactcggcag
    24541 gcacagcctg ctgttcgatg gcgtggccga attgctggac gcgatcgagg ccgccggcag
    24601 ccgctggggc atcgtcacca acaagccgga atacctggcg gtgcaggtca tgccggcgct
    24661 cggctgggac acccgcagcg cgatcctggt cggcggcgac accctgccgg aacgcaagcc
    24721 gcatccgttg ccgttgctgc attcggccga gcagctgggg gtcgcgatcg gggattgcgc
    24781 ctacgtcggc gacgaccagc gcgacatcgc gtccgcgcgc gcggccggga tgcgttcggt
    24841 ggtggcgctg tggggctacc gccgcgacga cgacgacccg cgggactggg gtggcgacgc
    24901 cctggccgac cgcgtgcacg acctgctgga tcctggcttg tgggcgtcgc tgcgatgagc
    24961 gacgtcgacg ccttgcaggc gttcctcgcc aagtggcggg cgcgctggcc ggaatgggcg
    25021 gtggccgaag tgttcgtgcc ggcggcccag cgcgagcgtg cgcaggcctg gttcgccctg
    25081 cgcgacgaac tcggcgacgc ggcgtggggc gggcaggacc cgctcccggg cgatgccaag
    25141 ctgggctggt gggccgagga actggatggc tggtcgcgcg gcgcgcgccg gcacccgctg
    25201 gggcttgcgt tgcagaaggt gcaggcgccc tgggacaacc tggcggcctg cctgccggcg
    25261 ctgcgcgcga gccgcgagcg cccggccaac cttgatggcg cgatcttcgc cctggagccg
    25321 tacgcagaag cggtggccgg ggttgcccag cacctgttcg acagtgtcgc cccggcgccg
    25381 gcgcgcaacg tggtgctgtc gctgctggcc gagcgcgcga tgcgccatcc ggacggcgcg
    25441 acgccgctgg gcgaccttga cgtccgcggc tggtcgcgcg ggttgctggc gcaatggccg
    25501 ccgcccggcg acggcacccg gccggggcgc atccatgccg cggtggtccg cggccgcctg
    25561 caacgccacg cgcagggcaa gccgccagcg ctgccgcggc ttggcgcact ggcgaccgcc
    25621 tggcgcgccg cccgcggctg atcgcgccag gacggggcac gcagcgttgc acgggtggtc
    25681 acctccgcgc cggtacaatg gcgcgatgca caaccgctcc cgtcattcgt cgctccccga
    25741 tgtcgccgcc gatgcgcccg cgctggcacg gccgctggac tgggtcggga tggacgcgat
    25801 ggccctgccg atccgcctcc agggcgagca cggccaggca ctgcacgtgc cggcacaggt
    25861 ggatgtcgcg gtcgaccttg gcgacgccgc cgcgcgcggc atccacatgt cgcggctgta
    25921 cctgcagctg caggagtcgc tggcgaacga ggcgctgagc gcgcccgcgc tgcgcaagtt
    25981 gctggaatcg tgcatcgcct cgcagcaggg attgtcgtcg cgtgcgcgcc tgcgcctgcg
    26041 ctacgaccac ctgttgctgc ggccggcgct ggccagtggc aacagcggct ggaagaatta
    26101 cccggtggcg ctggaggccg agttgcgcga cggccacctg cagctgtcgc tggcgttctc
    26161 ggtcgaatac tcgagcactt gcccggcctc ggcggcgctg tcgcggcagg tcaatgcgca
    26221 acgcttcgcc acggatttcg ccggggtgcg cccgctgtcg aacgatgcgg tgcgcgactg
    26281 gctggcatcg gagcgcgggc ttgccgcaac cccgcacgcg cagcgcagcc gcgccgagct
    26341 gcgggtggaa ctgaagccgg cattcgacga attgccgttg aaggcgctgg tggatgcggt
    26401 ggaagccgcg ctcggcacgc cggtgcagac cgcggtcaag cgcgaggacg aacaggcctt
    26461 cgccgagctc aacgcggcca acctgatgtt ctgcgaagac gccgcgcgcc gggtggccgc
    26521 cgcgctggcg acggatccgc gggtcagcgc gtggcaggtg cgggtggcgc atttcgagag
    26581 cctgcatccg cacgatgcgg tcgcatccgt gagtggcagg aacgcctgag gtccgccccc
    26641 acccttgcga agcaggggag ggttggggtg gggtgcggct ggtcgggatt cgcgacaaaa
    26701 gcacaccctc ccaacctcac cctgcgcttc gcgcaagcgg gggagacaag ggctacttgc
    26761 gctcaagcac cagcagcggg tcgatgcgca catcgaacca gttcatgccc cagtgcaggt
    26821 gcgccccggt ggcgcgtccg gtcgcgccca ccgcgccaat cacgtcgcct tgcttgacga
    26881 cgtcgccgac ccgcgcatcg atccgcgaca ggtgcaggaa attgctgctg atgccgtggc
    26941 cgtggtcgag caccatggtg ccgccggtca ggtagaggct ggcgtccgcg aagctgacca
    27001 cgccgtcggc gggcgccttg accggcgttc cgttcggcgc ggcgatgtcc atgcccgaat
    27061 gcggcgactt cggggtgccg ttgtacacgc gctggttgcc gaagcggccg ctgacgcggc
    27121 cctgcaccgg ccagatgaag gcttgcgcga aaccggtgcg ggcatcgtcg cggctgcgca
    27181 ccgcgaccac gcgcgcctgc tctcgctcga tgcgttcggc gatcgccttg ggtgggttca
    27241 cggtcgcggg cggcacgccg tcgatgcgct cgaccggcca gtcacgcggg gtgatggcga
    27301 cccggtgctc gatccagccg ctggccggtt gccggatccg cacgatagcc tcgccggtct
    27361 gatcgcggct gatgccgaat gcgaagctgc cgtacgtggt tacccgcagg ctgcggccgg
    27421 catgttccac ggtcgccgcc ggatgggtca cgcccagcac catcgcgccc tgctgcgcgg
    27481 aggcgggcag gcgggtggcg cgggccggga ccgcgccttc gccgccgacc atcggactgg
    27541 catcgcccgc aaccgactgc gcagcggcga tgccggcgag ggcgaagcag ggcagcagcg
    27601 ggagcaggac gcggcgcatc agcggtcgaa tcggaggcgc tggccggcag gatcgccgac
    27661 cagtgccgag ccgtcccagg ccagcacgcc gttgacccag gtcgcggcga tccgcgaacg
    27721 gaaggtggtg ccctcgaacg gcgaccagcc gcacctgctg agcacgtcgc cgcgttgcac
    27781 ggtgaacggg gtgtcgtcga tcatcacgag atcggctgcg taaccttcgc gcaggaagcc
    27841 gcggttggcc acgtcgaaca actgcgccgg cgcatgcgcg aacttctgga cgacctgggc
    27901 ggtgctcagc cggccttcgt gcaccagttc cagcgccgcg ttgagcgcga actgcaccag
    27961 cggcaggccg ctgggcgcag ccgcgtaggg cttcgccttt tcttccagcg tgtgcggggc
    28021 gtggtcggtg gcgaggacat cgatgcggtc gttggccagc gcgtcgatca gggcctggcg
    28081 gtcggcctcg tccttgatcg ccgggttgca cttgatcagg ttgccaagcc gcgcgtagtc
    28141 ggcgcgggcg aagcgcagga aatgcacgca ggtctcggcg gtgatcggct tgcggctgcc
    28201 gtcggcacgg atcagcgggc cctgctcgaa cagcgccagt tcgtcggcag tggagatgtg
    28261 caacacgtgc aattgggtgc cgtgccggcg cgcgagttcc agcgccagcc gggtcgactt
    28321 gatgcaggcc tcgcgggagc ggatgtccgg gtggcattcg accgggatgg cgtcgccatg
    28381 cttcgcccgg aacagcgcga ggttggcgtc gatggtcggg gtgtcctcgc agtgggtgat
    28441 gatcggcacc g
//
LOCUS       DAORUK010000005        27831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_1266_length_27831_cov_18.799755, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000005 DAORUK010000000
VERSION     DAORUK010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 27831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27831
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1266_length_27831_cov_18.799755"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="PK818_00805"
     CDS             complement(<1..1223)
                     /locus_tag="PK818_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY81306.1"
                     /translation="MRHRLLAVAILSSLALPAFAADTAAGPGPGQALQCGNLFDSRSG
                     KLLGAHTVVVRDGKVAEVLAGRADAVADATTTDLAGHTCMAGWTDLHVHMASQSSPQS
                     YSEGFRLDDVDFAFRSVGYAEKTLLAGFTSVRDLGGSVAPNLRDAINQGLVKGPRIWA
                     AGKSIATTGGHADPSNGVNDRLAHLIGPPGPTEGVINSIDDARQAVRQRYKDGSDLIK
                     ITATGGVLSYAKSGDAPQFTVDEVKAIVDTAKDYGYRVAAHAHGEEGMYRAVAGGVTS
                     IEHGSYMSDRVMALMKQKGTWYVPTIHAGRFVAEKAKIDGYFPDIVRPKAARIGAQIQ
                     DTAARAYRNGVKIAFGTDMGVGPHGDNAKEFLYMVEAGMPAAYALQAATIHAAQVLGV
                     DDQGVVEAGKRADIIA"
     gene            1322..4066
                     /gene="leuS"
                     /locus_tag="PK818_00810"
     CDS             1322..4066
                     /gene="leuS"
                     /locus_tag="PK818_00810"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HQY81307.1"
                     /translation="MPAVSEPAVYEPSAVESAAQSFWARTRAFEVNEQSDKPKYYCLS
                     MLPYPSGALHVGHVRNYTIGDVISRHKRMTGHNVLQPMGWDAFGLPAENAAIKNKTAP
                     AKWTYANIEHMRAQLQSLGYAIDWSREFATCKPDYYMHEQRMFVRLLKKGVAYRKNSV
                     VNWDPVDQTVLANEQVIDGRGWRTGALVEKREIPQWFLRITDYAQQLLDELDTLDGWP
                     DSVKTMQRNWIGRSEGLEIQFRVDGEAEPLTVFTTRPDTLMGVTFISVAGEHPLAIKA
                     AESNPELAAFLAELKQGGVSEAELETQDKRGMATGQWAIHPITGEDVPVYVANFVLMG
                     YGTGAVMAVPAHDQRDWEFAKAYGLPVKPVIVDDAVRDALQEMSSHVAEHDDPMAVAL
                     GAHAPVDVVEIDAAVEVVREYLRRIEEEGAQTERGWLVNSGEFNGMDFQAAFDALATR
                     LQADGRGARRVNFRLRDWGVSRQRYWGCPIPVIHCAACGAVPVPEGQLPVLLPEDVAD
                     AFAAGNVHSPIKSDPEWRKTTCPQCGGAAERETDTFDTFMESSWYYARYTSPGASDMV
                     DERANYWLPVDQYIGGIEHAILHLMYFRFYHKLLRDAGLVHSDEPATRLLTQGMVIAD
                     TFYREDELGAREWINPADVELQHDERMRVTGAKSLLDGLPVLIGGTEKMSKSKNNGVD
                     PQAMVDRYGADTVRLFSMFAAPPEQSLEWNESGVEGMARFLRRLWRDVHAHVAQPDHP
                     EVDANALDAGQKTLRRQTHEAIQKVADDYGRRHSFNTAIAALMELLNHLGKFTDMGDQ
                     GRAVRHEAFEAIVLLLNPITPHISHALWQVLGHPETLLEDLSFPQADPAALVRDALTL
                     AVQVNGKLRGTIEVAPDASRGATEAAALADPHVAAFVAGKEVRKVIVVPGKIVNIVVA
                     "
     gene            4191..4703
                     /gene="lptE"
                     /locus_tag="PK818_00815"
     CDS             4191..4703
                     /gene="lptE"
                     /locus_tag="PK818_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HQY81308.1"
                     /translation="MTRRLAPLILAATLLTGCGFHLRNALNLPANLGPVRVLASDPYS
                     ALAEDLANGLARAGAQAAAADASEGVATLQVVSEQWADTAISHDQFGRAQEFTLRYAV
                     IFSMRRADDSVVVPQQAVELGRDYLASPVDSIGKASERELLVRELRRDMAAAILRRVD
                     AASKPAAVAQ"
     gene            4812..5861
                     /gene="holA"
                     /locus_tag="PK818_00820"
     CDS             4812..5861
                     /gene="holA"
                     /locus_tag="PK818_00820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HQY81309.1"
                     /translation="MELNVDRIAPQLAAEPLRPAYLVAGQEPLLVIEAADAVRAAARA
                     QGIDEREVHDMDGRDADWDALSASMHAPSLFASRRLVEVRLPTGKPGNEGSKLLCEFC
                     AAPPADVVLLVIASDWSRAHAGKWTEAIGRIGHSCIAWQVKPHDLGSWIERRLRSRGV
                     KATPDAVARLVQRIEGNLLAAAQEIDKLALLVQGEGGVAETLDADAMESLVADSARFD
                     VFRLVDAALAGNPSQVSRTLAGLRAEGEAVPALMGMVVKELQVVASLARARNFAAECK
                     ALRIWDSKQALYKRALGKHPPERWQRFLAEAGRVDRIGKGRARVGEESTDAWLQLERL
                     LLAVAERGAGRLLAS"
     gene            5858..6562
                     /gene="nadD"
                     /locus_tag="PK818_00825"
     CDS             5858..6562
                     /gene="nadD"
                     /locus_tag="PK818_00825"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016944351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HQY81310.1"
                     /translation="MSAARLLVCYGGTFDPVHNGHLAVARAARDLLDADVALVPAHDP
                     PHKGPTRADALQRAEMLELALAGEPGLRVDRRELRRQGPSYTVDTLGELRRELGADAP
                     IAWLVGADSLLELHTWHRWRELFTLAHLVAVQRPGSEVDAARLRAQAPEVLAEIDGRW
                     LPPGDLAASPAGGFALLALPALRPESSTGLRRRIREGGDWQDWVAPAVAAFIDRHGLY
                     RGPADILPVSPHPSQR"
     gene            6573..6992
                     /gene="rsfS"
                     /locus_tag="PK818_00830"
     CDS             6573..6992
                     /gene="rsfS"
                     /locus_tag="PK818_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HQY81311.1"
                     /translation="MTTTAHVIKTRLPTPPPAVETLLDSVHAALEELKAKDMVRIDVR
                     GKSSVCDFMVIASGTSSRHVKSIADEVVRFAKKLDVMPLGVEGEREAEWVLVDLGDVV
                     VHVMLPRVREFYALERLWTVGDQPPGRDREGDAEAQP"
     gene            6997..7467
                     /gene="rlmH"
                     /locus_tag="PK818_00835"
     CDS             6997..7467
                     /gene="rlmH"
                     /locus_tag="PK818_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HQY81312.1"
                     /translation="MKARLVAVGERAPAWVAQGFCEYQKRLSHWLPLELVEVEPGVRG
                     KGRDTARATFDEGARVLAALPKNAHVVALDGRGKMHSSEQLAQRLQHWRSQGRDLAFL
                     VGGPEGHAAEVLQRADEAWSLGPLTLPHMLVRLVLAEQVYRAAALLANHPYHRA"
     gene            complement(7499..8203)
                     /locus_tag="PK818_00840"
     CDS             complement(7499..8203)
                     /locus_tag="PK818_00840"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HQY81313.1"
                     /translation="MDRALALPRPTGFALLEPASRPQLDAARILTLSGSIALNLLAIG
                     LLMMPIALPPPVVVAEQPRLPSYRQIIREPEVVPVVPVARPAPTTAARQASPPRTTQA
                     PDQRAAPAVVSDGGTEFVIEPGTSDGAGSATGIAPEATAPAPIQLEYRHAPAPAYPRR
                     ALQQRLAGTVLLRVLVGTDGYPLEVTVARSSGHRELDDAARVQVLKRWSFRPASGNGQ
                     PVQAIGLVPIEFALTR"
     gene            complement(8462..9211)
                     /locus_tag="PK818_00845"
     CDS             complement(8462..9211)
                     /locus_tag="PK818_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HQY81314.1"
                     /translation="MPLRPRVLATALVLGSLALGAAMTANAQPQAATAHAMPADGTLL
                     SVSATADARRVPDVATLSTGVVTQAADANAAMRANALQMDRVMAAVRAAGIAERDIQT
                     SGISLNPNYKYIENAAPAIVGYQASNTVNIRVRDLSRLGKVLDTLVEQGANQVNGPSF
                     EVDKPEAAYDEARIAAIKQAQARAQTYADALGMRVRRVVSISEGGAGFPRPMPMMRAM
                     AADAGMARETSVSPGESTLSVSLEVVFELGR"
     gene            9363..9932
                     /locus_tag="PK818_00850"
     CDS             9363..9932
                     /locus_tag="PK818_00850"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HQY81315.1"
                     /translation="MLHLASRSPRRAELLARLGLDFGVIDLDIPEHQGALEPASEYVR
                     RVAREKAGAGLLEVVAVPGAVVLGADTEVILDGRVFGKPADAGDAAEMLRALSGRTHE
                     VTTAVALVSAGREAQAVSTSQVRVARLSEADIDAYVACGEWAGKAGGYAIQGRFQAHV
                     EHLAGSYSGVMGLPLFETSRLLRQFGLPA"
     gene            9929..11422
                     /gene="rng"
                     /locus_tag="PK818_00855"
     CDS             9929..11422
                     /gene="rng"
                     /locus_tag="PK818_00855"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002802933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HQY81316.1"
                     /translation="MSEELLVNVTPRETRVAVVENGMLQELHIERGSQRGVVGNIYKG
                     KVQRVMPGMQAAFVDIGLDRAAFLHANDIFRSAQLDAGESDDAPAATPVQVQVPITEL
                     LRDGAEIVVQVVKDPIGSKGARLTTQISIPSRYLVLLPRSRVVGVSARIEGDDERARL
                     KALVAELAAPGNTHGYIVRTNAEGQPAEALAEDIAYLGRVWRLVEDTAAGASVGNCIY
                     EDLSLPLRAVRDLIRRDVEKVKVDSRETCERLREFAAQYMPELAEKIEHYSGARPIFD
                     LYGVEDEIGRALEKEVPLKSGGYLVIDQTEAMTTVDVNTGSFLGQRNLEETVYRTNLE
                     AAQSVARQLRLRNLGGIIIIDFIDMVDAEHRRQVLRTLEKQLARDHAKTTVYDFSPLG
                     LVEMTRKRTVESLQRQLSEPCHECSGRGTVKTAETVTYEVFREIVRAVRQFDAARLLV
                     IASPKVVARITEEESNAVAELEEFLGKSIRFQADAQYLQEQFDVVLL"
     gene            11450..15346
                     /locus_tag="PK818_00860"
     CDS             11450..15346
                     /locus_tag="PK818_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HQY81317.1"
                     /translation="MTEAHAHAIPPRRRWRLLRRGAWYALAAALVLMALGSGLASQLL
                     PVAERHPDEIARWLSARAQRPVAFDAVQTEWTRRGPLLRLDNLRIGAGAEALRIGDAE
                     ILVAQYAGLLPGRSFTELRVRGLDLVLQRDAGGRWSVRGLPGQQAGGDPFDTLGRLGE
                     LQVSHARLRVTAPELQIDMLLPRIDLRMRVDGPRIRGGARAWLREQATPFAMALDFDR
                     RSGDGRVYAGTRKADLGEFADSLRVAGIAPVSGRGRVQAWAGLQAHRVVSVHADAALE
                     DVVLRGAPLDAARPAPALDFGIVSLDARWSGSIRQWQASASRLRIGGGGREQVLDGLA
                     VAGGRHYGLRARRLDATPLIALLASSDRLAPGLRHWLQAAAAGAVLENVEVAGVRGGA
                     LRVDADVHGLHFAAVGNSPGMRGVDAQLRGDADGLRLRFEPGAPVAFDWPAGFGVEHE
                     VSLGGEAVLWREGAGWTVHTPGLAILGRQLDVNARGGIGFGNDGTRPRLDLAVDIGEA
                     PVTLARQFWVHHLMPKSTVDWLNAALQGGRLRDVHAVVAGDLDDWPFRSEPAATGPGR
                     AGAGKFRVDARIVDGLLKFQPDWPPMAAVDADIRFEADGFTIAGKGRLGGIPVTAFKA
                     GIPRFGRAELSVDAEGAGNASEFLAVLRRSPLRKDHAELLDNLRAAGPASTDFHLLVP
                     FHHQPDARGNSPRMQMQGTVELAGASLREERWKLAFDNVRGQARYDRDGFVAEGLQVR
                     HDGAPGVLSLRAGPHVRDPGQAFEAELQANVGIDVLLDKAGNLGWLKPWLDGRSPWTV
                     AVAIPRGASQAAAPARLQLRSNLVGTAIGLPEPVRKPAAEAMATTVDVSLPLERGEVA
                     VVLGNLLSLRSRSSAQQTGVRVQLGGSAAEAPPASGLVVGGRVARLDALDWIGVFAGE
                     GGNGSLPLRRIEVDARQLHLLGSSFADARLVLAPAARGTAVQVQAAGIAGSLLVPEQD
                     GATVAGRFERLFWNVPPGSGAGVGAPAKAASAAPPPSAGGFDPANIPPLLFEVDDLRI
                     GSAALGSARFRSTPVAGGMRMDEFSSRGARQRLTAGGTWSGRGGAARTQVKVDVDSDD
                     IGALLAGLGFGGQVAGAGGKLGLEANWRGGPEAFDPTVMDARLQVDARDGRLLEIEPG
                     AGRVLGLLGIAQLPRRLTLDFRDFFDKGFAFDRITGEVRLTQGVARTDDLTIRGPAAD
                     IHVRGSTDLRSQRFDQTVDVLPKSGGLLTAVGALAGGPVGAAVGAVANAVLDKPLQGL
                     GAKTYRVTGPWAAPKVEVTARSPAAQARPARDPPAG"
     gene            15397..16839
                     /gene="tldD"
                     /locus_tag="PK818_00865"
     CDS             15397..16839
                     /gene="tldD"
                     /locus_tag="PK818_00865"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007972594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HQY81318.1"
                     /translation="MTHLAIAESRLLAPTGLDLDGLQRAFGTLLGPGIDFGDLYFQHS
                     RRENWSMEDGIVKDGAHSIEQGVGVRAISGEKTGLAYSDDLDAQALIASAQSARAIAR
                     DGREHVAHALQRSGGRRLYPALDPVDALGNAAKVAALRRVDGLLRALDPRVQQVMVGL
                     SGSVDTVLVARSDGVLAGDVRPLVRMNVQVIVEQDGRRESGYAGFGGRYSYEDLLADD
                     KPEKFAREALRQALVNLDAVDAPAGVMPVVLGPGWPGVLLHEAVGHGLEGDFNRKGTS
                     TFTGRIGQQVASKGVTIVDDGTLDGRRGSLNIDDEGTPTSCTTLIEDGVLVGYMQDTL
                     NARLMGMAATGNGRRESFAHLVMPRMTNTYMRAGSHDPQDMIRSVKRGLYAVNFGGGQ
                     VDITSGKYVFSATEAYLIEDGKVTAPVKGATLIGNGPETMQKVAMIGHDMKLDDGVGV
                     CGKDGQSVPVGVGQPSLLISELTVGGTRAG"
     gene            complement(16840..17424)
                     /gene="yjgA"
                     /locus_tag="PK818_00870"
     CDS             complement(16840..17424)
                     /gene="yjgA"
                     /locus_tag="PK818_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HQY81319.1"
                     /translation="MRGRDENTGEFLGPSRSQQRREALDILELGERLVALTAAQLARL
                     PVPDELLPHIRETQRITSHGARKRQLAFLAKQMRRQDDAALDAIRDALGKDGEAARRE
                     TAAMHRVEALRDSLLGDDGDAAMTALLGEFAGADRQKLRQLVRNTHEERKRNKPPHAF
                     RELYRELRELTAQDDAVADQPADDMFDEGDEGHA"
     gene            17442..18809
                     /gene="pmbA"
                     /locus_tag="PK818_00875"
     CDS             17442..18809
                     /gene="pmbA"
                     /locus_tag="PK818_00875"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HQY81320.1"
                     /translation="MNSPISQARPADDSRERLQHLSAIAERLLERCKAAGASQAEVSC
                     NEDTGLAVNVRMGEVETVEATRDRGIAVTVYFGQRKGSASTADQREASLDATVAQACA
                     IAGFTEDDAAAGLADPALMATTQREFDGWHPWDIDAQRAIDLALACEQAGRDSDPRVS
                     NSDGASVNSNASLSVYANSHGFLGGERSTSHSIGCALIAGEGEAMQRDSWYSAALAQS
                     DLESAADVGRKAAQRTVERLQPRHLPTAEVPVLFHAEIARSLVAHYVSAISGGALYRR
                     ASFLMDSVGQRVFPDWFGIDELPFLARGFRSAAFDAEGVATRESPLVREGVVQRYVLG
                     SYSARKLGLATTANAGGVHNLQVHANAGDLESMLRGMHRGLLVTDLMGQGVNPITGDY
                     SRGAAGFWVEGGRIVHPVDGITIAGNLKQVFAAIEAVGSDIDPRSHLRSGSILVGRMT
                     VAGSG"
     gene            18896..19318
                     /locus_tag="PK818_00880"
     CDS             18896..19318
                     /locus_tag="PK818_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HQY81321.1"
                     /translation="MDLNEATAPPPPPPSGMPGQEERQWAMFAHLSALLGGLLTSVIG
                     GWGFFLGPLVIWLMKKDTMPFVADQAKEALNFNITVSAIFLLLLILSFLTLGIGFLIT
                     VPIMLVVGLGALVLVIMAAIKANEGVAYRYPFAIRLVK"
     gene            19464..19865
                     /locus_tag="PK818_00885"
     CDS             19464..19865
                     /locus_tag="PK818_00885"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HQY81322.1"
                     /translation="MEAINMQTLSPSLSDRQWAAAAHAAALFAALLTSWVAGVAGAVA
                     ALAVWMLVRDKSAFAAEHAKEAVNFNLSMFIYAALAVVLVVFTLGIGLILAIPLWIVL
                     ALVWIVCTLVAAFKAYDGQAYRYPVTIRLFK"
     gene            complement(19879..21243)
                     /locus_tag="PK818_00890"
     CDS             complement(19879..21243)
                     /locus_tag="PK818_00890"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HQY81323.1"
                     /translation="MTRPANQAWGWLLGLALAGAMLAAVAATPATTATAATVARPGVK
                     AHAKPDFASPEVATLEQAAAVQVTGQQGLWFKLALAGGKSGYVRVNDVRVAYASKQGG
                     GIGKALFTGKAGKGRVTETASVRGIDESALKSASFDAERLQEMESYRATPEAAEEAAG
                     KRGWQATLVPYAVEYQPRPAKPGKQGKPRASQAETRERFGIAGKLASHMGSAFGGVLS
                     SGSKLVGKSEQEIVQEELELGPMIAGRILGAVPLLQDPAAQQRVNLVGRWLASRSSRP
                     ELPWTFGVIDDGEINAFAAPGGYILVTRGLYQLLENDAEVAAVLAHELSHVVQRDHYE
                     VLRKQELQGAGKELVSSQVRTGGGLAGSVARGYIERNGAAIMLTQLDRNAEYRADQAA
                     GIYLARAGTNPLAYYAVLQKMASLGTSSPRLTHLYKTHPPLDKRLDALDANGYANLQA
                     YLDR"
     gene            complement(21240..23108)
                     /locus_tag="PK818_00895"
     CDS             complement(21240..23108)
                     /locus_tag="PK818_00895"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HQY81324.1"
                     /translation="MARRQVRAAQQSVDTLMASLAVWFMEAWTVVERGWRRPLTRVAL
                     RLKFAFYPLLACAALGWLGWDWSHDRSLDAAEDSIFDTAIQWRPVEPRPSGQTVVVEI
                     DDCSIEWTRAQGLGGWPWPRQVHANLLDALDRSGVRAVGVDVMFLDPDNDDPAGDAML
                     EAVAEGGNGRFVFAASRQDPAFDAGSPLRASRIPGAFALDPAAAAADPAVAVYLPFAA
                     AMARHSALTNVSRSQDGVLRDVPLREPVGHWALPTLPARLAAGVRGVSPASLPPHVRV
                     NWRKHSRVPFVSAADLLAGTPVCVAPGAAPPVLRGAVALVGYTAAGLSDSKPTPVDAS
                     MPGVEVWAEATDALLHDSAIWMPPTVLKYLLAALLVALTCYAFWRGEPHEDVDGVFVA
                     SNLVLLGCAFIGLTFFGIFLDIFASIGFVSLCFGLCRLYAAVQRGRAVGNNDYRAEYD
                     PRRHPWMVMARLRFVRAPGLAPFAAMRGKREYRRLLRRRLYAGGDAVMIEGIVERKSW
                     LHDILDDLVLLVWTGSERVATLETAKRELDALHHDLNADELTLDEHGRVLVSFSVAEI
                     DDDDDSDRLGERVRLRELLGQDLNARDEFPLQADNREVLDPVHTLDLDDTMKETDA"
     gene            complement(23121..24083)
                     /locus_tag="PK818_00900"
     CDS             complement(23121..24083)
                     /locus_tag="PK818_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQY81325.1"
                     /translation="MPEAAFADWRARVEAALDHALPAVDQPPHRLHAAMRHAVLNGGK
                     RMRPLLVYATGTAFGAPAQALDAAAAAVELVHCYSLVHDDLPAMDDDALRRGQPTVHV
                     AFDDATAILAGDALQALAFEVLAGAPQDPRQRVAMLAELARAAGAAGMCGGQALDIDA
                     TGQAAKRGQGHVSLEENDSAQHPDRQHDPGPFSFSLADLERLHALKTGALLRASVRLG
                     AIAAGADAASRAALDRYADALGLAFQVRDDLLDIEGDAATLGKTAGKDLAQAKATFPA
                     LLGIEASRGRLQSLSATMQEELRALPTDGTWLAALARKVVERSH"
     gene            complement(24076..24333)
                     /locus_tag="PK818_00905"
     CDS             complement(24076..24333)
                     /locus_tag="PK818_00905"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HQY81326.1"
                     /translation="MAKKPAPESSPVAQFEASMQALEDLVGRMEAGEMSLEESLAAYE
                     RGVGLYRSCQAALEQAELRVRLLSDPAQPGDARPFESFDDA"
     gene            complement(24404..25705)
                     /gene="tilS"
                     /locus_tag="PK818_00910"
     CDS             complement(24404..25705)
                     /gene="tilS"
                     /locus_tag="PK818_00910"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HQY81327.1"
                     /translation="MTHPHAPLRRSVPTQGGAVVVGFSGGMDSTVLLHLLANDTAIRR
                     EGLRALHVHHGLQAGADAWAGHCDQACMALGVPLTLVRVRVDRDSGLGPEGAARAARH
                     RAFAQALGDGEILALAHHRDDQAETFLLRALRGSGVDGLAAMRHWRRYAGGWLWRPLL
                     AQPRVALHAYARAHGLHWVEDPGNAGSDPDRNFLRHQVLPLLRERWPHAAHSLARSAV
                     LCAEAADLLDAGDALALADARRDRDTLDVEVLQALPRARRARVLRHWVLALQLPPLPG
                     NGVDRIEAALLGARADAQASFDWSGARVQRWRNLLHAGRIRAPLPLRWEQDWDGQAPL
                     ALPTGDRLQLLGTDRFDESIRVHARRGGERIRLPRRSHQHSLKQVLQARGIPPWQRVR
                     MPLLSDREGLLAAGDGILSARLQAWLQERGAGLLWRQGPGG"
     gene            complement(25746..26033)
                     /locus_tag="PK818_00915"
     CDS             complement(25746..26033)
                     /locus_tag="PK818_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81328.1"
                     /translation="MVIKRIDPLSAAKIGGIIAAAIGLLAGVVFFLVSSLFGAALGQQ
                     EGGGLAMFGGAMGMVLLPILYGIFGFIGGLIQAFIYNLAAGFVGGIRIETE"
     gene            complement(26104..27066)
                     /locus_tag="PK818_00920"
     CDS             complement(26104..27066)
                     /locus_tag="PK818_00920"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HQY81329.1"
                     /translation="MNPNYLDFEQPIADLEAKIQELRHASNGPAVNIDAEVHALQDKL
                     RKRTATIFRDLTPWQVSQLARHPSRPYTLDYLRVICDEFQELAGDRAFADDNAIVGGL
                     ARIDGRPLMVIGHEKGRDTKAKVRRNFGMPKPEGYRKALRLMKLAERFGLPVLTFIDT
                     AGAWPGIDAEERGQSEAIARNLLEMAELRVPIICTVIGEGGSGGALAIGVGDRTLMLE
                     YSTYSVITPEGCASILWRDAGKAKDAAEQLGLTAKRLSGLGLVDKVVREPTGGAHRNP
                     VQMAKRLKAVLLNELDALQGVPVEDLLQRRYQRLRGYGAYETAS"
     gene            27168..>27831
                     /locus_tag="PK818_00925"
     CDS             27168..>27831
                     /locus_tag="PK818_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HQY81330.1"
                     /translation="MDATPVQAAEPGLQADAGVPWHASLRTRLMLWVGLLLALLLLAA
                     IATAFYAARSRIVADAEARTRFEAQQAADRLDATMRSVRISGESLIDLGNRVELGREQ
                     LLAAMEAMLDANPGTVGGLVALEPGVLADRGRLAYYVGIAARGVPDRDLLADGYDLVG
                     REWYQRTLQATSPWWSEPYFSETAGGRYMTTLNLPLRNREAKRIGMVSLDVPVQALSS
                     SLE"
BASE COUNT         3960 a         9632 c        10112 g         4127 t
ORIGIN      
        1 gcgatgatgt cggcgcgctt gccggcttcg accacgccct ggtcgtccac gcccaacacc
       61 tgcgcagcgt ggatggtcgc cgcctgcagc gcatacgcgg cgggcatccc ggcttcgacc
      121 atgtagagga attccttcgc gttgtcgccg tgcgggccca cgcccatgtc ggtgccgaag
      181 gcgatcttca ccccgttgcg gtaggcccgc gccgcggtgt cctggatctg tgcaccgatg
      241 cgcgcggcct tgggccgcac gatgtccggg aagtagccgt cgatcttcgc cttctcggcc
      301 acgaagcggc cggcatggat ggtcggcacg taccaggtgc ccttctgctt catcagcgcc
      361 atcacccggt cgctcatgta gctgccgtgc tcgatgctgg tcacgccgcc ggcgacggcg
      421 cgatacatgc cttcctcgcc gtgcgcgtgc gcggcgacgc gatagccgta gtccttcgcg
      481 gtatcgacga tcgccttgac ctcgtcgacg gtgaactgcg gcgcgtctcc ggacttcgca
      541 tagctcagca cgccgccggt ggcggtgatc ttgatcaggt cgctgccgtc cttgtagcgc
      601 tggcgcacgg cctggcgggc gtcatcgatg gaattgatca cgccctcggt ggggccgggc
      661 ggaccgatca ggtgcgccag ccggtcgttg acgccgttgc tgggatccgc gtgcccgccg
      721 gtggtggcga tcgacttgcc ggcggcccag atccgcggtc ccttgaccag cccctggttg
      781 atcgcgtcgc gcaggttcgg ggccacgctg cccccgaggt cgcggacgct ggtgaaaccc
      841 gcgagcaggg tcttttccgc atagccgacg ctgcggaagg cgaaatccac gtcgtccagg
      901 cggaaccctt cggaatagct ctgcgggctg gactggctgg ccatgtgcac gtgcaggtcg
      961 gtccagccgg ccatgcaggt gtggccggcg aggtcggtgg tggttgcgtc cgccaccgcg
     1021 tccgcgcgcc ctgccagcac ttcggccacc ttgccatcgc gcaccaccac ggtgtgcgcg
     1081 cccagcagct tgccgctgcg gctgtcgaac aggttcccgc actgcagcgc ctggcctggt
     1141 cccgggccgg cggcggtgtc cgcggcgaag gcgggcaggg cgagcgagga caggatggcg
     1201 acggcaagca ggcgatggcg catggcggtt cccccggggc gatggccgat ggtgccacgc
     1261 aaacccgctg catggcggtg gatgctgcgc tgcggtaggc ttcccgaccc gattcccgtt
     1321 gttgcccgcc gtgtccgaac ccgccgtcta cgaaccctcc gccgtcgaat ccgccgccca
     1381 gtcgttctgg gcccgcacgc gtgcgttcga ggtgaacgag cagtccgaca agccgaagta
     1441 ctactgcctg tcgatgctgc cgtacccgtc cggcgccctg catgtcggcc acgtgcgcaa
     1501 ctacaccatc ggcgacgtga tcagccgcca caagcgcatg accggccaca acgtgctgca
     1561 gccgatgggc tgggacgcgt tcggcctgcc ggccgagaac gccgcgatca agaacaagac
     1621 cgcgccggcc aagtggacct acgccaacat cgagcacatg cgcgcgcagt tgcagtcgct
     1681 gggctacgcg atcgactgga gccgcgagtt cgccacctgc aagccggact actacatgca
     1741 cgagcagcgc atgttcgtgc gcctgctgaa gaagggcgtg gcctaccgca agaacagcgt
     1801 ggtcaactgg gatccggtcg accagaccgt gctggccaac gagcaggtga tcgacggccg
     1861 cggctggcgc accggcgcgc tggtcgagaa gcgcgagatc ccgcaatggt tcctgcgcat
     1921 caccgattac gcccagcagt tgctggacga actggacacg ctcgatggct ggccggattc
     1981 ggtcaagacc atgcagcgca actggatcgg ccgcagcgaa ggcctggaga tccagttccg
     2041 ggtcgatggc gaagccgagc cgctcacggt gttcaccacc cgcccggaca ccctgatggg
     2101 cgtgaccttc atcagcgtgg ccggcgaaca tccgctggcg atcaaggcag cggaaagcaa
     2161 tcccgaactg gccgcgttcc tggccgagct caagcagggc ggcgtgtccg aagccgaact
     2221 ggagacccag gacaagcgcg gcatggccac cgggcagtgg gcgatccacc cgatcaccgg
     2281 ggaagacgtc cccgtctacg tcgccaactt cgtgctgatg ggctacggca ccggcgcggt
     2341 gatggcggtg ccggcgcacg accagcgcga ctgggaattc gccaaggcgt acggcctgcc
     2401 ggtgaagccg gtgatcgtgg atgacgcggt gcgcgacgcg ctgcaggaaa tgtcctcgca
     2461 cgtcgccgag cacgacgacc cgatggcggt cgcgctgggc gcgcatgcgc cggtggacgt
     2521 ggtcgagatc gatgcggcgg tcgaagtcgt gcgcgagtac cttcgccgca tcgaggaaga
     2581 aggcgcgcag accgaacgcg gctggctggt caattccggc gagttcaacg gcatggactt
     2641 ccaggccgcg ttcgatgcgc tggccacccg cttgcaggcc gatggccgcg gcgcgcggcg
     2701 ggtgaatttc cgcctgcgcg actggggcgt gagccgccag cgctattggg gctgcccgat
     2761 cccggtcatc cactgcgccg cctgcggcgc ggtgccggtg ccggaagggc aactgccggt
     2821 gctgctgccg gaagacgtgg ccgacgcctt cgccgccggc aacgtgcatt cgccgatcaa
     2881 gtccgatccg gaatggcgca agaccacctg cccgcaatgc gggggcgcgg ccgagcgcga
     2941 gaccgacacc ttcgacacct tcatggagtc gagctggtac tacgcgcgct acacctcgcc
     3001 gggcgcgagc gacatggtcg acgaacgcgc caactactgg ctgccggtcg accagtacat
     3061 cggcggcatc gagcacgcga tcctgcacct gatgtacttc cgcttctatc acaagctcct
     3121 gcgcgacgcc gggctggtgc acagcgacga acctgccacg cgtttgctga cccagggcat
     3181 ggtcatcgcg gacaccttct atcgcgagga cgagctcggc gcgagggaat ggatcaaccc
     3241 cgccgatgtc gaattgcagc acgacgagcg catgcgcgtg accggcgcga agtcgctgct
     3301 cgatggcctg ccggtgctga tcggcggcac cgagaagatg tcgaagtcga agaacaacgg
     3361 cgtcgatccg caggcgatgg tggaccgcta cggcgccgac accgtgcgcc tgttctcgat
     3421 gttcgccgcg ccgccggagc agtcgctgga gtggaacgag tccggcgtgg aaggcatggc
     3481 ccgcttcctg cgtcgcctgt ggcgcgacgt gcatgcgcat gtcgcccagc ccgaccatcc
     3541 cgaagtcgat gcgaatgcgc tcgatgccgg gcagaagacc ctgcgccggc agacccacga
     3601 ggcgatccag aaggtcgccg acgattacgg ccgccgccac agcttcaaca ccgcgatcgc
     3661 cgcgctgatg gagctgctca accacctcgg caaattcacc gacatgggcg accagggccg
     3721 cgcggtgcgc cacgaggcgt tcgaggccat cgtgctgctg ctgaacccga tcaccccgca
     3781 catcagccac gcgctgtggc aggtcctggg ccacccggaa acgctgttgg aagacctgtc
     3841 gttcccgcag gccgatcccg ccgcgctggt gcgcgatgcg ctgaccctgg cggtgcaggt
     3901 caacggcaag ctgcgcggca ccatcgaggt cgcgcccgac gcctcgcgcg gggcgaccga
     3961 ggcggccgcg ctggccgacc cgcacgtggc cgcattcgtg gccggcaagg aggtcaggaa
     4021 ggtcatcgtg gtgccgggca agatcgtcaa tatcgtggtg gcgtgacgcg gcccgtcatt
     4081 cccgcgcagg cgggaatccc gggactttgc ccttcacgcc gataacgtcc atggatcccc
     4141 gccgtcgcgg ggatgacggc actgatccag acatgacata aactgccccg atgacccgac
     4201 gcctcgcccc gctcatcctc gccgccacgc tgctcaccgg ctgcggcttc cacctgcgca
     4261 atgcgctgaa cctgccggcc aacctcggcc cggtgcgggt gctggcgtcg gatccttaca
     4321 gcgcactggc ggaggacctg gccaatgggc tcgcgcgcgc cggcgcgcag gcggcggcag
     4381 ccgacgccag cgagggcgtg gccaccctgc aggtggtgtc cgaacagtgg gcggacaccg
     4441 cgatcagcca cgaccagttc ggccgcgcgc aggagttcac cctgcgctac gcggtgatct
     4501 tctccatgcg ccgcgccgac gacagcgtgg tggtgccgca gcaggcggtg gaactggggc
     4561 gcgattacct tgcatcgccg gtggattcga tcggcaaggc gagcgagcgc gaactgctgg
     4621 tgcgcgaact gcgccgcgac atggccgcgg cgatcctgcg ccgggtcgat gctgcctcca
     4681 agccggccgc cgtcgcgcag tgagcggaaa cggcaacgaa acggggccag agtcatggtt
     4741 tcggtctgcg ggttccgcct gctggttgtt ctcgaccgaa aaatgactct ggctcctttt
     4801 cgcctttcgt catggaactg aacgtcgacc gcatcgctcc gcagctggcc gccgagccgc
     4861 tgcgcccggc gtacctggtc gccgggcagg agcccctgct ggtcatcgaa gccgccgatg
     4921 cggtgcgcgc cgccgcgcgc gcacaaggca tcgacgagcg cgaagtgcac gacatggatg
     4981 gccgcgacgc cgactgggat gcgctgtccg cgtcgatgca cgcgcccagc ctgttcgcca
     5041 gccggcgcct ggtggaggtg cggctgccga ccggcaagcc cggcaacgag ggcagcaagc
     5101 tgctctgcga attctgcgcg gcaccgcccg cggacgtggt gttgctggtg atcgccagcg
     5161 actggtcgcg tgcgcatgcc ggcaagtgga ccgaggcgat cggccgcatc ggccattcct
     5221 gcatcgcctg gcaggtcaag ccgcacgacc tcggcagctg gatcgaacgc cgcctgcgca
     5281 gccgcggggt caaggccacg ccggacgcgg tggcgcggct ggtccagcgg atcgaaggca
     5341 acctgctggc cgcggcccag gaaatcgaca agctcgcgtt gctggtgcag ggcgagggcg
     5401 gcgtggcgga gacgctggat gccgacgcga tggagtcgct ggttgccgat tccgcgcgct
     5461 tcgacgtgtt ccgcctggtc gatgccgcgc tcgccggcaa cccgtcgcag gtttcgcgca
     5521 cgctcgccgg gctgcgcgcc gagggcgagg cggtgccggc gctgatgggc atggtggtca
     5581 aggaactgca ggtcgtcgcc agcctggcgc gggcgcgcaa tttcgcggcc gagtgcaagg
     5641 cgctgcggat ctgggattcc aagcaggcgc tgtacaagcg cgcgctgggc aagcacccgc
     5701 cggagcgctg gcagcggttc ctggccgaag ccgggcgcgt cgaccggatc ggcaagggcc
     5761 gggcccgggt cggcgaggaa tccacggacg cctggctgca gctggaacgc ctgctgctgg
     5821 cggtggccga gcgcggtgcc ggcaggctgc tggcgtcgtg agcgcggcac ggctgctggt
     5881 ctgctacggc ggcaccttcg atccggtcca caacggccac ctggcggtcg cgcgcgcggc
     5941 gcgcgacctg ctggacgccg atgtcgccct ggtccccgcg cacgatccgc cacacaaggg
     6001 cccgacccgc gccgatgccc tgcagcgcgc ggagatgctg gagctggcgc tggcgggtga
     6061 acccggcctg cgggtggacc ggcgcgaact gcgccggcag gggccgtcgt acaccgtcga
     6121 cacacttggc gagctgcgcc gcgagctcgg cgccgacgcc ccgatcgcgt ggctggtcgg
     6181 cgccgactcg ctgctggaac tgcacacctg gcatcgctgg cgcgaactgt tcacccttgc
     6241 gcacctggtc gcggtgcaac ggccgggctc cgaggtcgat gccgcgcgcc tgcgtgcgca
     6301 ggcgccggag gtgctggccg agatcgacgg tcgctggcta ccgccgggcg acctggccgc
     6361 gagtcctgcc ggcggcttcg ccctgctggc gttgccggcg ttgcggccgg aatcgtccac
     6421 cgggctgcgc cggcgcatcc gcgaaggcgg cgactggcag gactgggtgg cgccggcggt
     6481 ggccgccttc atcgaccgcc acgggctcta ccgcgggccg gcggatatac tgcccgtgtc
     6541 cccgcatccc tcccagagat gagcgcccgc ccttgaccac caccgcccac gtcatcaaga
     6601 cccgcctgcc cacgccgcca cccgcggtgg aaacgctcct cgacagcgtg cacgccgcgc
     6661 tcgaggaact gaaggccaag gacatggtcc ggatcgacgt acgcggcaag agcagcgtct
     6721 gcgatttcat ggtgatcgcc tccggcacct cgtcgcgcca cgtcaagtcg atcgccgacg
     6781 aagtggtgcg tttcgccaag aagctcgacg tgatgccact gggcgtcgaa ggcgagcgcg
     6841 aggccgaatg ggtgctggtc gacctcggcg acgtggtggt gcacgtgatg ctgccgcgcg
     6901 tgcgcgagtt ctacgcgctg gagcggttgt ggacggtcgg cgaccagccc cccggacgcg
     6961 accgcgaagg cgacgccgaa gcgcagccct gaggctttga aggcgaggct ggtcgcggtt
     7021 ggcgaacgcg cacccgcatg ggtggcgcag gggttttgcg aataccagaa gcgcctctcg
     7081 cactggttgc cgctggaact ggtggaagtc gaacccgggg tgcgtggcaa gggccgcgac
     7141 accgccaggg ccaccttcga cgagggtgcc cgtgtgcttg cggccttgcc gaagaacgcg
     7201 catgtggtcg cgctcgacgg gcgcgggaag atgcattcct ccgaacagct ggcgcagcgg
     7261 ctgcagcact ggcgcagcca gggccgcgac ctggcgttcc tggtcggcgg cccggagggc
     7321 catgccgcgg aggtgctgca gcgggccgat gaagcatggt cgctggggcc gctgaccctg
     7381 ccgcacatgc tggtgcggct ggtgctggcc gagcaggtct accgcgccgc cgcgctgctg
     7441 gcgaaccacc cgtaccaccg ggcctgaaac ccggtggcgg gcgacgccgt cggcacgcct
     7501 accgcgtcag cgcgaattcg atcggcacca ggccgatcgc ctgcaccggc tggccgttgc
     7561 cggaggccgg ccggaagctc cagcgcttca gcacctgcac ccgcgcggcg tcgtccagtt
     7621 cgcgatggcc gctgctgcgc gcgacggtga cctcgagcgg gtagccgtcg gtgccgacca
     7681 gcacccgcag cagcacggtt ccggcgagcc gctgctgcag cgcacgccgc ggatacgcgg
     7741 gtgccggggc gtgccggtat tcgagctgga tcggcgcggg agcggtggct tcgggcgcga
     7801 tgccggtggc cgagccggcg ccgtcgctgg tgccgggctc gatcacgaac tcggtaccgc
     7861 cgtcgctgac caccgcgggt gccgcgcgct ggtcgggggc ctgggtggtg cgcggcggcg
     7921 aggcctggcg ggcggcggtg gtgggcgccg gccgcgccac cggcaccacc ggcacgacct
     7981 ccggctcgcg gatgatctgg cggtaactcg gcagccgggg ttgttcggca acgaccaccg
     8041 gcggcggcag cgcgatcggc atcatcaaca gcccgatcgc gagcaggttg agcgcgatcg
     8101 agccgctcag ggtgaggatg cgggcggcgt cgagttgcgg ccgggaagcg ggttccagca
     8161 gggcgaagcc ggtcgggcgg ggcagggcga gggcacgatc catggtcaac ctccggtcgg
     8221 gtgcggtggc gggcatgcgc ggtggaagcc ggcgcgaacc atgcaacgga cgggtgcagg
     8281 cgatggggtt ggcgcccgcc gattgcatgg atcgacgctg cgccgtgcag ttgcaggcgg
     8341 caaccacgtg gcgacggccg gcgatggcgt ggcgaccaac ggcgcgatgc cgcggccatg
     8401 caaacgaacg cccgcgttgg cgggcgtcgt tgttggttcg atgcgcgttt ccgttacccg
     8461 gtcagcgccc cagttcgaac accacctcca ggctgaccga aagcgtgctc tcgcccggcg
     8521 aaaccgaggt ttccctggcc atccccgcgt cggccgccat tgcccgcatc atcggcatcg
     8581 ggcgcgggaa tccggcgccg ccctcgctga tgctgaccac ccggcgcacc cgcatgccca
     8641 gcgcgtcggc gtaggtctgc gcccgcgcct gcgcctgctt gatcgcggcg atgcgcgcct
     8701 cgtcgtaggc cgcctccggc ttgtcgacct cgaagctggg gccgttgacc tggttggcgc
     8761 cctgctcgac cagggtgtcc agcaccttgc ccagcctgga caggtcgcgc acccggatgt
     8821 tgaccgtgtt gctggcctgg tagccgacga tcgccggtgc cgcgttctcg atgtacttgt
     8881 agttcgggtt gaggctgatg ccgctggtct ggatgtcgcg ctcggcgatg ccggcggcgc
     8941 gcaccgcggc catcaccctg tccatctgca gtgcgttcgc gcgcatcgcc gcgttggcgt
     9001 ctgcggcctg ggtgaccacg ccggtggaga gggtggccac gtccggcacg cgcctggcgt
     9061 cggccgtcgc ggacaccgac agcagggtgc catcggcggg catcgcatgg gcggtcgcgg
     9121 cctggggctg ggcattggcg gtcattgcgg ctccaagggc aagtgatccg aggacgaggg
     9181 cggtggcgag gacgcgcggg cgaagcggca tggtggactc ctgggggatg cggtggaagg
     9241 gtgcggcaag tgcgggggcg gacggtgaac gcactgcgcg tggtgaacgc atggtgagcc
     9301 ggcgcggggt tgcgtgcgcc gtcgcacatg gcctgcagtc agccgcgggt tagacttcgt
     9361 gcatgctgca tcttgcctcg cgctcgccgc gccgcgccga actgctggcc cgcctggggc
     9421 tggacttcgg cgtcatcgac ctcgacatcc cggagcacca gggggcgctg gaacctgcgt
     9481 ccgaatacgt gcgccgggtc gcgcgcgaaa aggccggtgc cggcctgctg gaggtggtcg
     9541 cggtccccgg tgcggtggtg ctgggtgccg ataccgaggt catcctcgac ggccgcgtgt
     9601 tcggcaagcc ggccgatgca ggcgatgccg cggagatgct gcgcgcgctc tccggccgca
     9661 cccacgaggt gaccaccgcg gtggcgctgg tgtcggccgg gcgcgaggcg caggcggttt
     9721 cgacctcgca ggtgcgggtg gcacggcttt cggaggccga catcgatgcc tacgtggcct
     9781 gcggcgaatg ggccggcaag gctggcggct atgcgatcca gggccgcttc caggcccatg
     9841 tcgagcacct ggccggcagt tacagcgggg tgatgggcct gccgctgttc gagacatcgc
     9901 gcctgctcag gcaattcggg ctgccggcat gagcgaggag ctgctggtca acgtgacccc
     9961 gcgcgagacc cgggtggccg tggtcgagaa cggcatgctg caggagctgc acatcgagcg
    10021 cggcagccag cgcggggtgg tcggcaacat ctacaagggc aaggtgcagc gggtgatgcc
    10081 gggcatgcag gccgcgttcg tggacatcgg cctggaccgc gcggcattcc tgcacgccaa
    10141 cgacatcttc cgcagcgccc agctggacgc cggcgaaagc gacgacgcac ccgccgcgac
    10201 cccggtgcag gtgcaggtcc cgatcaccga actgctgcgc gacggggccg agatcgtggt
    10261 gcaggtggtc aaggatccga tcggcagcaa gggcgcgcgg ctgaccaccc agatcagcat
    10321 cccgtcgcgc tacctggtgc tgttgccgcg ttcgcgggtg gtcggcgtgt cggcgcggat
    10381 cgagggcgac gacgaacgcg cgcgcctgaa ggcgctggtg gccgaactgg ccgcgccggg
    10441 caatacccat ggctacatcg tgcgcaccaa tgccgaaggc cagccggccg aagcgctcgc
    10501 cgaggacatc gcctacctcg ggcgggtgtg gcggctggtc gaggacaccg ccgccggcgc
    10561 cagcgtcggc aactgcatct acgaggacct gagcctgccg ctgcgcgcgg tccgcgacct
    10621 gatccggcgc gacgtcgaga aggtcaaggt cgactcgcgc gagacctgcg agcgcctgcg
    10681 cgagttcgcg gcgcagtaca tgccggagct ggcggaaaag atcgagcact acagcggcgc
    10741 gcgcccgatc ttcgacctgt acggggtcga ggacgagatc ggccgcgcac tggaaaagga
    10801 ggtgccgctg aagtcgggcg gctacctggt gatcgaccag accgaggcga tgaccacggt
    10861 cgacgtcaac accggcagct tcctcggcca gcgcaacctg gaggaaaccg tctaccgcac
    10921 caacctggag gcggcgcagt cggtggcgcg ccagctgcgg ctgcgcaacc tgggcgggat
    10981 catcatcatc gacttcatcg acatggtcga tgccgaacac cgccggcagg tgctgcgcac
    11041 gctggaaaag cagctggccc gcgaccacgc caagaccacg gtgtacgact tctcgccgct
    11101 ggggctggtg gagatgaccc gcaagcgcac cgtcgagtcg ctgcagcgcc agctcagcga
    11161 accctgccac gagtgcagcg ggcgcggcac cgtgaagacc gcggaaaccg tgacctacga
    11221 agtgttccgc gagatcgtgc gcgcggtgcg ccagttcgac gcggcgcggc tgctggtgat
    11281 cgcttcgccg aaggtggtgg cgcggatcac cgaggaggaa tcgaacgcgg tcgccgagct
    11341 ggaggaattc ctgggcaagt cgatccgctt ccaggcggat gcccagtacc tccaggagca
    11401 gttcgacgtg gtcctgctct gatcggcggc cccaccctcg tgaacgccgt tgactgaagc
    11461 gcacgcccac gccatccccc cgcgtcgccg ctggcggctg ttgcggcgtg gcgcctggta
    11521 cgcgctggcc gcggcgctgg tactgatggc gcttggcagc ggcctggcca gccagctgct
    11581 gccggtcgcc gaacgccatc ccgacgagat cgcgcgctgg ctcagcgcgc gcgcgcaacg
    11641 cccggtggcg ttcgatgcgg tgcagaccga gtggacccgg cgcgggccgc tgctgcggct
    11701 ggacaacctg cggatcggcg cgggcgccga agcgctgcgg atcggcgatg cggagatcct
    11761 ggtggcgcag tacgccggcc tgctgcccgg gcgttcgttc accgaactgc gggtgcgcgg
    11821 gctggacctg gtcctgcagc gcgatgccgg cgggcgctgg agcgtgcgcg gactgccggg
    11881 gcagcaggcc ggcggcgatc cgttcgacac cctgggacgg ctgggtgagt tgcaggtctc
    11941 gcacgcgcgg ctgcgggtga ccgccccgga gctgcagatc gacatgctgc tgccgcggat
    12001 cgacctgcgc atgcgggtgg acgggccacg gatccgcggc ggtgcgcgcg cgtggctgcg
    12061 cgagcaggcc accccgttcg cgatggcgct ggacttcgac cgccgcagcg gcgacggccg
    12121 ggtctacgcc ggcacccgca aggccgacct gggcgaattc gccgacagcc tgcgcgtcgc
    12181 cggcatcgcg ccggtgtccg gccgcggtcg ggtgcaggcc tgggccggat tgcaggcgca
    12241 ccgggtggtc agcgtgcatg ccgatgccgc actggaagac gtggtgctgc gcggtgcgcc
    12301 attggatgcc gcgcggccgg cgcctgcgct ggatttcggg atcgtcagtc tcgacgcgcg
    12361 ctggtccggc agcatccgcc agtggcaggc cagcgcaagc cggctgcgga tcggcggtgg
    12421 cgggcgcgaa caggtgctgg atggcctcgc ggtggccggc ggccggcact acggcctgcg
    12481 cgcgcggcgc ctcgatgcca ccccgttgat cgcgctgctc gcgtcaagcg accggctggc
    12541 gccgggcttg cggcactggt tgcaggccgc tgcggccggc gcggtgctgg aaaacgtgga
    12601 ggtggccggc gtgcgtggcg gtgcgctgcg ggtggatgcg gacgtgcacg gcctgcactt
    12661 cgcggccgtc ggcaacagcc ccggcatgcg cggcgtggat gcacagttgc gcggcgacgc
    12721 cgatggcttg cgcctgcgct tcgagcctgg cgcgccggtg gcgttcgact ggccggccgg
    12781 tttcggggtg gaacacgagg tcagcctcgg tggcgaggcg gtgctgtggc gcgagggcgc
    12841 cggctggacc gtgcacacgc cggggcttgc catcctcggc cgccagttgg acgtcaacgc
    12901 gcgcggcggc atcggcttcg gcaacgatgg cacccgcccg cggctcgacc tggcggtcga
    12961 catcggcgaa gcgccggtga ccctcgcgcg ccagttctgg gtccaccacc tgatgccgaa
    13021 atcgacggtg gactggctca acgccgcgct ccaggggggg cggctgcgcg acgtgcacgc
    13081 ggtggtggcc ggcgacctcg acgactggcc gttccgcagc gagccggccg ccaccgggcc
    13141 gggccgggcg ggtgccggca agttccgggt ggatgcgcgg atcgtcgatg gcctgctgaa
    13201 gttccagccg gactggccgc cgatggcggc ggtggatgcc gacatccgct tcgaggccga
    13261 tggcttcacc atcgccggca aggggcgcct tggcgggatc ccggtgaccg cgttcaaggc
    13321 cggcattccg cgtttcgggc gcgccgaact gagcgtcgac gcggaaggcg ccggcaatgc
    13381 cagcgagttc ctcgcggtgc tgcgccgcag cccgctgcgc aaggaccacg ccgagctgct
    13441 ggacaacctg cgcgcggcag ggccggccag caccgacttc cacctgctgg tgccgttcca
    13501 ccaccagccg gatgcacggg gcaacagccc gcggatgcag atgcagggca cggtcgaact
    13561 ggctggcgcc agcctgcgcg aggagcgctg gaagctggcg ttcgacaacg tccgcgggca
    13621 agcacgctac gaccgcgacg ggttcgtcgc cgaaggcctg caggtgcgcc acgatggcgc
    13681 gcccggcgtg ctgtcgttgc gcgccggccc gcacgtgcgc gaccccggcc aggccttcga
    13741 ggcggagctg caggccaatg tcggcatcga tgtcctgctc gacaaggccg gcaacctcgg
    13801 ctggttgaaa ccctggctcg acgggcgctc gccgtggacg gtggcggtgg cgatcccgcg
    13861 cggtgcatcg caggctgcgg cgccggcccg cctgcaattg cgttcgaacc tggtcggcac
    13921 cgcgatcgga ctgccggaac cggtgcgcaa gcctgccgcg gaggcgatgg cgaccacggt
    13981 ggacgtgagc ctgccgctgg aacgcggcga ggtggcggtg gtgcttggca acctgttgtc
    14041 gctgcgcagc cgcagcagcg cgcagcagac cggcgtgcgc gtgcaactgg gcggcagcgc
    14101 cgcggaggca ccgccggcca gcgggctggt ggtcggcggg cgggtggcgc ggctggacgc
    14161 gctggactgg atcggcgtgt tcgccggtga aggcggcaac gggtcgctgc cgctgcggcg
    14221 gatcgaggtc gacgcaaggc agctgcacct gctcggttcc agcttcgccg atgccaggct
    14281 ggtgctggcg ccggcggcgc gcgggaccgc ggtgcaggtg caggccgcgg gcatcgccgg
    14341 ctcgctgctg gtgccggagc aggacggcgc gaccgtcgcc ggccgcttcg aacggctgtt
    14401 ctggaacgtg ccgccgggga gcggcgcggg ggtcggcgcg ccggcgaagg ctgcctccgc
    14461 cgcaccaccg ccgtccgcgg gcggattcga tccggccaac atcccgccgc tgctgttcga
    14521 agtggacgac ctgcggatcg gcagcgccgc gctgggtagc gcgcgcttcc gcagcacgcc
    14581 ggtcgccggc ggcatgcgca tggacgagtt cagctcgcgc ggcgccaggc agcggctcac
    14641 cgccggcggc acctggtcgg gccgcggcgg cgcggcgcgc acgcaggtca aggtggacgt
    14701 ggacagcgac gacatcggcg cgctgctcgc cggacttgga ttcggcggcc aggttgccgg
    14761 tgctggcggc aagctcgggc tggaggcgaa ctggcgcggt gggcccgagg cgttcgaccc
    14821 gacggtgatg gatgcacggc tgcaggtgga cgcgcgcgac ggccgcctgc tcgagatcga
    14881 gcccggcgcc gggcgcgtgc tcggcttgct cgggatcgcg caactgccgc gccggctgac
    14941 cctcgatttc cgcgacttct tcgacaaggg cttcgccttc gaccgcatca ccggcgaggt
    15001 gcggctgacg cagggggtgg cgcgaaccga cgacctgacg atcaggggcc cggccgcgga
    15061 catccacgtg cgcggcagca ccgacctgcg cagccagcgc ttcgaccaga ccgtggacgt
    15121 gctgccgaag tcgggtggcc tgctgaccgc ggtcggcgcg ctcgccggtg ggccggtcgg
    15181 tgcggcggtc ggtgcggttg ccaatgcggt gctggacaag ccgctgcagg gcctcggcgc
    15241 caagacctac cgggtcaccg ggccgtgggc cgcgccgaag gtcgaggtca ccgcgcgcag
    15301 cccggcggcg caggcgcggc ccgcgcgcga tccacctgcc ggttgatccg cgcgggttcg
    15361 ccgccatctc cccatccagc ccaacgaagc gcccccatga cccatctcgc cattgccgaa
    15421 tcgcgcctgc tcgcccccac cggactcgac ctggacggcc tgcaacgcgc cttcggcacc
    15481 ttgctcggcc ccgggatcga cttcggcgac ctgtatttcc agcattcgcg gcgcgagaac
    15541 tggagcatgg aggacggcat cgtcaaggac ggcgcgcatt cgatcgaaca gggcgtgggc
    15601 gtgcgtgcga tctccggcga gaagaccggc ttggcctact ccgacgatct cgacgcgcag
    15661 gcgttgatcg cctccgcgca gtccgcgcgg gcgatcgcgc gcgacggacg cgaacacgtt
    15721 gcgcacgcct tgcagcgcag cggggggcgc aggttgtacc cggcgctcga cccggtcgac
    15781 gcgctcggca acgcggccaa ggtggcggcg ttgcgccgcg tcgacggcct gctgcgcgcg
    15841 ctggatccgc gcgtgcagca ggtgatggtg ggtctcagcg gcagcgtcga cacggtgctg
    15901 gtggcgcgca gcgacggcgt gctggccggc gacgtgcgcc cgctggtgcg gatgaacgtg
    15961 caggtgatcg tcgagcagga tggtcgtcgc gagtccggtt acgccgggtt cggcgggcgc
    16021 tattcgtacg aagacctgct ggccgacgac aaaccggaaa agttcgcgcg cgaagcattg
    16081 cgccaggcgc tggtgaacct cgacgcggtg gatgcgcccg ccggggtgat gccggtggtg
    16141 ctgggcccgg gctggccagg cgtgctgctg cacgaggcgg tcggccacgg gctggaggga
    16201 gacttcaacc gcaagggcac ctcgaccttc accggccgca tcggccagca ggtggcgtcg
    16261 aagggcgtga ccatcgtcga cgacggcacc ctcgacggcc gccgcggttc gctcaacatc
    16321 gacgacgaag gcaccccgac gtcctgcacc accctgatcg aggacggcgt gctggtcggt
    16381 tacatgcagg acaccctcaa cgcgcggctg atggggatgg cagccaccgg caacggccgc
    16441 cgcgaatcgt tcgcgcacct ggtgatgccg cgcatgacca atacctacat gcgcgccggc
    16501 agccacgacc cgcaggacat gatccgctcg gtgaagcgcg gcctgtacgc ggtgaatttc
    16561 ggtggtggcc aggtcgacat caccagcggc aagtacgtgt tctcggcgac cgaggcctac
    16621 ctgatcgagg acggcaaggt caccgcgccg gtgaagggcg ccaccctgat cggcaacggc
    16681 ccggagacca tgcagaaggt ggcgatgatc ggccacgaca tgaagctgga cgatggcgtc
    16741 ggcgtgtgcg gcaaggacgg gcagtcggtg ccggtgggcg tgggccagcc ctcgctgctg
    16801 atctccgagc tgaccgtcgg cggcacccgc gcgggctgat caggcgtgtc cttcgtcgcc
    16861 ttcgtcgaac atgtcgtccg ccggctggtc cgcgacggcg tcgtcctgcg ccgtcaactc
    16921 gcgcagttcg cggtacagct cgcggaacgc atgcggcggc ttgttgcgct tgcgctcctc
    16981 atgcgtgttg cgcaccagct ggcgaagctt ctggcggtcg gcgccggcga actcgcccag
    17041 cagcgcggtc atcgccgcgt cgccgtcatc gccgagcagg ctgtcgcgca gcgcttcgac
    17101 gcggtgcatc gccgcggttt cgcggcgcgc ggcctcgccg tccttgccca gtgcatcgcg
    17161 gatcgcatcc agcgccgcgt cgtcctgccg gcgcatctgc ttggccagga acgccagctg
    17221 ccgcttgcgc gcgccatggg aggtgatgcg ctgggtctcg cggatgtgcg gcagcagctc
    17281 gtcggggacc ggcagccgcg ccagttgcgc cgcggtcagc gccaccagcc gttcgcccag
    17341 ctccaggatg tccagcgcct cgcgccgttg ctggctgcgg ctgggcccca ggaattcgcc
    17401 ggtgttttcg tcgcgtcctc gcatcgccac aggataagcc attgaacagt ccgatcagcc
    17461 aggcccgccc cgccgacgac agccgggaac ggctgcaaca cctgtccgcc atcgccgaac
    17521 gcttgctgga gcgctgcaag gcggccggcg ccagccaggc cgaggtcagc tgcaacgagg
    17581 acaccggcct tgcggtcaac gtgcgcatgg gcgaggtgga gacggtggag gccacccgcg
    17641 accgcgggat cgcggtgacc gtgtatttcg gccagcgcaa gggcagcgcc agcaccgccg
    17701 accagcgcga ggccagcctc gacgccaccg tggcccaggc ctgtgcgatc gccggcttca
    17761 ccgaggacga tgccgccgcc ggcctcgccg atccggcgct gatggcgacc acccagcgcg
    17821 aattcgatgg ctggcatccg tgggacatcg acgcgcagcg cgcgatcgac ctcgccctcg
    17881 cctgcgagca ggcggggcgc gacagcgacc cgcgggtctc caattccgac ggcgcctcgg
    17941 tcaacagcaa cgccagcctc agcgtctatg ccaactccca tggcttcctc ggcggcgaac
    18001 gcagcacctc gcacagcatc gggtgcgcgc tgatcgccgg cgagggcgag gccatgcagc
    18061 gcgacagctg gtacagcgcg gctctggcgc agtccgacct cgagagcgcc gcggacgtcg
    18121 gccgcaaggc ggcgcagcgc accgtggagc ggctgcagcc gcggcacctg ccgaccgccg
    18181 aggtgccggt gctgttccat gccgagatcg cgcgctcgct ggtcgcgcac tacgtcagcg
    18241 cgatttccgg cggcgcgctg taccgcaggg ccagtttcct gatggacagc gtcggccagc
    18301 gcgtgttccc cgactggttc ggcatcgacg agttgccgtt ccttgcgcgc gggttccgtt
    18361 ccgccgcgtt cgatgccgaa ggcgtggcca cccgcgaatc gccgctggtg cgcgaagggg
    18421 tggtgcagcg ctacgtgctg ggcagttact ccgcccgcaa gttgggcctt gcgaccaccg
    18481 ccaacgccgg tggcgtgcac aacctgcagg tgcatgcgaa tgccggggac ctggaatcga
    18541 tgctgcgcgg gatgcaccgc ggcctgctgg tgaccgacct gatggggcag ggcgtcaatc
    18601 cgatcaccgg cgattactcg cgcggtgccg ccgggttctg ggtcgaaggc gggcgcatcg
    18661 tgcatccggt ggacggcatc accatcgccg gcaacctcaa gcaggtgttc gccgcgatcg
    18721 aggcggtcgg cagcgacatc gacccgcgtt cgcacctgcg ttcggggtcg atcctggtcg
    18781 gcaggatgac ggtggccggc agcggttgac ctgcgccagc cgttggccgt tcgtcatgcc
    18841 gggtcgcgtt atcctcggca tcgcggtgtc ttgccgccaa taggggaatc cacgcatgga
    18901 cctgaacgaa gcaaccgcac cgccgccgcc tcctccctcc ggaatgcctg ggcaggaaga
    18961 acgccagtgg gcgatgttcg cgcacctgtc cgccctgctc ggtggcttgc tgacgtcggt
    19021 gatcggcggc tggggcttct tcctcgggcc gctggtgatc tggctgatga agaaggacac
    19081 catgccgttc gtcgccgacc aggccaagga agcgctcaac ttcaacatca cggtttcggc
    19141 gatcttcctg ttgctcttga tcctgagctt cctgaccctc ggcattggtt tcctgatcac
    19201 ggtgccgatc atgctggttg tcggccttgg cgccctggtc ctggtgatca tggcggcgat
    19261 caaggccaac gaaggcgtgg cctaccgcta cccgttcgcg atccgcctgg tcaagtaggc
    19321 gtccgtcgcc gccggcgggc gcctcgtcgc ccgctggcgg gtttcatcgc atcgtggcgg
    19381 tctccggtcg ccggctggct tgcatcgccg gccgcgatcc ccatcatccg atcaagccgg
    19441 tctggttccg gcgggagccc acgatggaag cgatcaacat gcagaccctg tccccgagcc
    19501 tgagcgaccg ccagtgggcg gccgccgcgc atgccgcggc attgtttgcc gcgctgctga
    19561 ccagttgggt ggcgggcgtg gctggcgcgg ttgccgcgct ggcggtgtgg atgctggtcc
    19621 gcgacaagtc cgcgttcgcc gccgaacacg cgaaggaggc ggtcaacttc aacctcagca
    19681 tgttcatcta tgccgcgctg gcggtggtgc tggtggtgtt caccctgggc atcggcctga
    19741 tcctcgcgat cccgctgtgg atcgtgctgg cgctggtgtg gatcgtgtgc accctggtcg
    19801 ccgcgttcaa ggcctatgac ggccaggcct accgttaccc ggtcacgatc cggctgttca
    19861 agtaatcgcg cgccggcgtc agcggtccag gtaggcctgc aggttggcgt agccattggc
    19921 atcaagcgca tccagccgct tgtcgagcgg cggatgcgtc ttgtacaggt gggtcaggcg
    19981 tggcgagctg gtgcccagcg aggccatctt ctgcaggacc gcgtagtacg ccagcggatt
    20041 ggtgccggcg cgcgccaggt agatgcccgc ggcctggtcc gcgcggtact ccgcattgcg
    20101 gtcgagctgg gtcagcatga tcgccgcgcc gttgcgctcg atgtagccgc gcgccacgct
    20161 gccggccaag ccgccaccgg tcctgacctg tgacgacacc agctccttgc cggcgccctg
    20221 cagctcctgc ttgcgcagca cttcgtaatg gtcgcgctgc accacgtggc tcagctcatg
    20281 cgccagcacc gcggcgacct cggcatcgtt ctccagcagc tggtagaggc cgcgggtgac
    20341 caggatgtag ccgccgggcg cggcgaaggc gttgatttcg ccgtcgtcga tcaccccgaa
    20401 ggtccacggc agttccgggc ggctgctgcg ggacgccaac cagcgcccga ccaggttcac
    20461 ccgttgttgc gcggccgggt cctggagcag cgggacggcg ccgaggatgc gccctgcgat
    20521 catcggcccc agttccagtt cctcctgtac gatttcctgc tccgacttgc cgaccagctt
    20581 gctgccggag gagagcacgc caccgaaggc ggaccccatg tgcgaggcca gcttgccggc
    20641 tatcccgaag cgttcgcgcg tttccgcctg cgatgcccgc ggcttgccct gcttgccggg
    20701 cttggccggg cgcggctggt attcgacggc gtagggcacc agggtggcct gccacccgcg
    20761 cttgccggcg gcttcctcgg cagcttccgg ggtggcccgg tacgactcca tctcttgcag
    20821 gcgttcggcg tcgaagctcg ccgacttcag cgcgctttcg tcgatgccgc gcacgctggc
    20881 ggtttcggtg acccggccct tgccggcctt gccggtgaac agcgccttgc cgatcccgcc
    20941 gccctgcttg ctggcgtagg ccacgcgcac gtcgttcacg cgcacgtagc cgctcttgcc
    21001 gcctgccagc gccagcttga accacaggcc ctgctgcccg gtcacctgca ccgccgcggc
    21061 ctgctcgagg gtggcgacct ccggtgatgc gaagtccggc ttggcatgcg ccttgacccc
    21121 gggcctggca acggtggccg ctgtcgcggt tgtcgccggg gtggccgcga ccgccgccag
    21181 catggcgccg gcaagggcga gtccgagcag ccagccccag gcctggttcg ccggtcgggt
    21241 catgcgtccg tctccttcat ggtgtcgtcc aggtccagcg tatgcacggg gtcgagcacc
    21301 tcgcggttgt cggcctgcaa cgggaattcg tcccgcgcgt tcaggtcctg gccgagcaac
    21361 tcgcgcaggc gcacgcgttc gcccaagcgg tcgctgtcgt catcatcgtc gatttccgca
    21421 acggagaacg acaccagcac ccggccgtgc tcgtcaagcg tcagctcgtc ggcgttcagg
    21481 tcgtggtgga gcgcgtccag ctcgcgcttg gcggtttcca gggtggccac gcgctcgctg
    21541 ccggtccaca ccagcagcac caggtcgtcg aggatgtcgt gcagccagct cttgcgttcg
    21601 acgatgccct cgatcatcac cgcgtcgccg ccggcataca gccgccgccg cagcaggcgc
    21661 cggtactcgc gcttgccgcg catcgccgcg aatggcgcca gcccgggcgc gcggacgaag
    21721 cgcaggcgcg ccatcaccat ccacgggtgg cggcgcggat cgtactcggc gcggtagtcg
    21781 ttgttgccca ccgcgcgccc gcgctgcacg gcggcataca gccggcacag gccgaagcac
    21841 aggctgacga aaccgatgct ggcgaagatg tccaggaaga tcccgaagaa cgtcaggccg
    21901 atgaaggcgc agcccagcag caccaggttg ctggccacga acacgccgtc cacgtcctcg
    21961 tgcggctcgc cgcgccagaa tgcgtagcag gtcaacgcca ccagcagcgc ggccagcagg
    22021 tacttgagca cggtcggtgg catccagatc gcgctgtcgt gcagcagcgc atcggtggct
    22081 tcggcccaga cctcgacccc gggcatcgac gcgtcgaccg gggtcggctt gctgtcgctg
    22141 agcccggcgg cggtgtagcc caccagcgcc accgcgccgc gcaggaccgg cggcgccgcg
    22201 cctggcgcga cgcagaccgg cgtgcccgca agcaggtcag ccgcgctcac gaacggcacc
    22261 ctgctgtgct tgcgccagtt cacccgcaca tgcggcggca acgatgccgg cgacaccccc
    22321 cgcacgcccg cggccagccg cgccggcagg gtgggcagcg cccagtggcc gaccggttcg
    22381 cgcagcggca cgtcgcgcag cacgccatcc tggctgcggc tgacattggt cagcgccgaa
    22441 tggcgggcca tcgccgccgc gaacggcagg tacaccgcga ccgccggatc cgccgctgcc
    22501 gccgcggggt caagtgcgaa ggcgcctggg atgcgcgaag cgcgcagcgg cgagccggcg
    22561 tcgaacgccg ggtcctggcg cgaggcggcg aacacgaagc ggccattgcc gccttccgcc
    22621 accgcttcca gcatcgcgtc gccggccggg tcgtcgttgt cgggatcgag gaacatcacg
    22681 tccacgccga ccgcgcgcac gccggaccga tccagcgcat ccagcaggtt cgcgtgcacc
    22741 tggcgcggcc acggccagcc gccgaggccc tgggcgcggg tccattcgat cgagcagtcg
    22801 tcgatctcca ccaccacggt ctggccggag gggcggggtt ccaccggccg ccactggatc
    22861 gcggtatcga agatgctgtc ttcggccgcg tccaggctgc ggtcgtggct ccagtcccag
    22921 cccagccagc cgagcgcggc gcaggccagc agcgggtaga aggcgaattt cagccgcaac
    22981 gccacccgcg tgagcggacg ccgccagccg cgttcgacca cggtccaggc ctccatgaac
    23041 cagaccgcaa gcgacgccat gagcgtgtcg acgctttgct gcgcggcccg gacctgcctg
    23101 cgtgccatgg atcccctgcc tcagtgcgag cgttcgacaa ccttgcgtgc cagtgccgcc
    23161 agccatgtgc cgtcggtcgg aagcgcgcgc aattcctcct gcatggtcgc agaaagggac
    23221 tgcaagcggc cacggctggc ctcgatgccg agcagcgcgg gaaacgtggc cttggcctgc
    23281 gcgaggtcct tgccggcggt cttgccgagg gtggcggcgt cgccttcgat gtccagcagg
    23341 tcgtcgcgga cctggaacgc caggcccagc gcatcggcgt agcggtcgag cgcggcccgg
    23401 ctggccgcgt cggctccggc ggcgatcgcg cccagccgca ccgacgcccg caacagcgcg
    23461 ccggtcttga gcgcgtgcag gcgttcgagg tccgcaagcg agaacgaaaa ggggccaggg
    23521 tcatgttgcc ggtcggggtg ctgcgccgaa tcgttctctt cgagcgaaac atgaccctgg
    23581 ccccttttcg ctgcctggcc ggtggcatcg atgtccagcg cctggccgcc gcacatgccg
    23641 gcggcgccgg ccgcgcgcgc cagttcggcc agcatcgcca cccgctgccg cgggtcctgt
    23701 ggcgcgccgg caagcacctc gaaggccagc gcctgcagcg catcgccggc caggatcgcg
    23761 gtggcgtcgt cgaaggcgac gtgcacggtg ggctggccac ggcgcagggc atcgtcgtcc
    23821 atcgccggca ggtcgtcgtg caccagcgag tagcagtgca ccagttccac cgcggctgcc
    23881 gcagcgtcca gcgcctgtgc cggcgcgccg aacgcggtgc cggtggcgta gacgagcagc
    23941 gggcgcatgc gcttgccgcc attgagcacg gcgtgccgca tcgccgcgtg caggcggtgc
    24001 ggcggctggt cgaccgcggg cagggcgtgg tcgagtgcgg cttcgacgcg cgcgcgccag
    24061 tccgcgaaag ccgcctcagg catcgtcgaa cgattcgaac gggcgcgcat cgcctggctg
    24121 cgcgggatcg ctgagcaggc ggacccgcag ctcggcctgc tcaagcgccg cctggcagct
    24181 gcgataaagg ccgacgccgc gctcgtaggc ggccagcgat tcctccagcg acatctcgcc
    24241 ggcctccatg cggccaacca ggtcctcgag cgcctgcatc gaggcctcga actgggcaac
    24301 cggggaggat tcgggagcgg gtttcttcgc catccatcga gtttacaagc tgcgcccggc
    24361 aaaccccaac ggccggcgcg tggccatccc gccgtgcgcc ggttcagccg ccgggaccct
    24421 gccgccacag caggccggcg ccgcgttcct gcagccaggc ctgcaggcgc gcggagagga
    24481 tgccatcgcc ggcggcaagc aggccctcgc gatcggacag caacggcatc cgcacgcgct
    24541 gccacggcgg gatgccgcgc gcctgcagga cctgcttgag cgaatgctgg tggctgcgcc
    24601 gcggcaggcg gatgcgttcg ccgccgcgcc gcgcatgcac ccggatcgat tcgtcgaagc
    24661 gatccgtgcc gagcagttgc aggcggtcgc cggtcggcag cgccagcggc gcctggccat
    24721 cccagtcctg ctcccaccgc aacggcagcg gtgcgcggat gcggccggca tgcagcaggt
    24781 tccgccagcg ctgcacgcgc gcgccggacc agtcgaagct ggcctgcgca tcggcacgcg
    24841 cgccgagcag cgcggcttcg atgcgatcga cgccgttgcc cggcagtggc ggcaattgca
    24901 gcgccaggac ccagtggcgc agcacgcgcg cgcggcgcgc gcgcggcagc gcctgcagga
    24961 cctcgacgtc gagggtgtcg cggtcgcggc gcgcatccgc cagcgccagc gcatcgcccg
    25021 cgtccagcag gtccgcggcc tccgcgcaca ggaccgcgct gcgcgcgagg ctgtgcgcgg
    25081 cgtgcggcca gcgttcgcgc agcagcggca ggacctggtg gcgcaggaag ttgcggtcag
    25141 gatcgctgcc ggcgttgccg gggtcctcga cccagtgcag gccgtgcgcg cgtgcgtacg
    25201 cgtgcagggc gacacgtggc tgcgcaagca gcggccgcca cagccagccg cctgcgtagc
    25261 ggcgccagtg gcgcatcgcg gccagcccat ccacgccgga gccgcgcagt gcgcgcagca
    25321 ggaaggtctc ggcctggtcg tcgcggtggt gggcgagggc gaggatctcg ccatcaccca
    25381 gcgcctgcgc gaacgcgcga tggcgtgcgg cgcgcgccgc gccctcgggg ccgaggccgg
    25441 aatcgcggtc gacgcggacg cggaccaggg tcaacggtac gcccagggcc atgcaggcct
    25501 ggtcgcaatg cccggcccag gcatcggcgc ccgcttgcag gccatggtgc acatgcagcg
    25561 cacgcaggcc ctcgcgacgg atcgcggtgt cgtttgcgag caggtgcagc aggacggtgg
    25621 aatccatccc gccgctgaag ccgaccacga ccgcgccgcc ctgcgtgggc acggatcgcc
    25681 ggagtggggc atgggggtgg gtcatccagc gctccacgcc ggcgtgcggc gcggagccgg
    25741 gtggctcatt cggtttcgat gcggatcccg ccaacgaagc cggccgccag gttgtagatg
    25801 aacgcctgga tcaggccgcc gatgaagccg aagatgccgt agaggatcgg caacaggacc
    25861 atgcccatgg cgccgccgaa catcgccagc ccgccgcctt cctgctgccc gagcgcggca
    25921 ccgaacaggc tgctgaccag gaagaacacc acgcccgcga gcaggccgat cgcggccgcg
    25981 atgatgccgc cgatcttggc ggccgaaagt gggtcgatac gcttgatgac cattgcccgt
    26041 tttccctggt tgcgccggcg atgtgccggc gcccgattct agccagatgc aggcttcgcc
    26101 ggatcaggac gcggtttcgt acgcgccgta gccacgcaac cgctggtagc ggcgctgcag
    26161 caggtcttcg accggcacgc cctgcagcgc gtccagttcg ttcagcagca ccgccttcag
    26221 ccgcttcgcc atctgcaccg ggttgcggtg cgcgccgccg gtcggttccc gcaccacctt
    26281 gtcgaccagc ccgagcccgg acagccgctt ggcggtcagg cccagttgtt cggcggcgtc
    26341 cttggccttg ccggcgtcgc gccacaggat cgaggcgcaa ccttccgggg tgatcaccga
    26401 gtaggtgctg tattccagca tcagggtgcg gtcgccgacg ccgatcgcca gcgcgccgcc
    26461 gctgccgcct tcgccgatca ccgtgcagat gatcggcacc cgcagctccg ccatctccag
    26521 caggttgcgg gcgatcgctt ccgattgccc gcgttcctcc gcatcgatcc ccggccacgc
    26581 gccggcggtg tcgatgaagg tcagcaccgg cagcccgaag cgctcggcca gcttcatcag
    26641 ccgcagcgcc ttgcggtagc cctccggctt cggcatgccg aagttgcggc gcaccttggc
    26701 cttggtgtcg cggcccttct cgtggccgat caccatcagc gggcggccat cgatccgggc
    26761 caggccaccg acgatggcgt tgtcgtcggc gaacgcgcgg tcgccggcga gttcctggaa
    26821 ctcgtcgcag atcacccgca ggtagtccag cgtgtacggc cgcgacggat gccgcgccag
    26881 ctgcgagacc tgccacgggg tgaggtcgcg gaagatggtg gcggtgcgct tgcgcagctt
    26941 gtcctgcagc gcgtgcacct cggcgtcgat gttgaccgcc gggccgttgc tggcgtgccg
    27001 cagttcctgg atcttggctt ccaggtcggc gatcggctgt tcgaagtcga ggtagttcgg
    27061 attcatccgc cgaagtttag cccagcgcgc ccgccatacc gaaccgtagg tcgcggcgcc
    27121 cgcccgcggc gcttgacgcc ggcgacgcgc catcggacgc tgcggccatg gacgcgacgc
    27181 ccgtgcaggc tgcggaaccg ggactgcagg cggatgccgg cgtgccctgg catgccagcc
    27241 tgcgcacccg gctgatgctg tgggtcgggc tgctgctggc gctgttgctg ctggccgcga
    27301 tcgccaccgc gttctacgcc gcgcgcagcc gcatcgtcgc cgacgccgag gcgcgcaccc
    27361 gcttcgaggc gcagcaggcc gccgaccggc tggacgcgac catgcgctcg gtgcgcattt
    27421 ccggcgaaag cctgatcgac ctcggcaacc gggtcgaact gggccgcgag cagttgctgg
    27481 cggcgatgga ggcgatgctg gacgccaatc cgggcacggt gggcgggctg gtggcgctgg
    27541 aacccggggt gctcgcggac cgtgggcggc tggcctacta cgtcggcatc gccgcgcgcg
    27601 gggtgccgga ccgcgacctg ctcgccgatg gctacgacct ggtcgggcgc gagtggtacc
    27661 agcgcaccct gcaggcgacc tcgccgtggt ggtcggagcc gtacttcagc gaaaccgcgg
    27721 gcgggcgtta catgaccacc ctcaacctgc cgctgcgcaa ccgcgaggcg aagcggatcg
    27781 gcatggtcag cctggacgtg ccggtgcagg cgctgtcgtc gtcgctggaa t
//
LOCUS       DAORUK010000006        27676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_1276_length_27676_cov_17.184461, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000006 DAORUK010000000
VERSION     DAORUK010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 27676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27676
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1276_length_27676_cov_17.184461"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PK818_00930"
     CDS             <1..434
                     /locus_tag="PK818_00930"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HQY81331.1"
                     /translation="DPMAMKPFCGYNFADYFAHWLSFDTEGAKLPKVFHVNWFRKGDD
                     GKFLWPGFGDNLRVLEWMIRRVEGGAGAVETPIGRLPAEADLNLEGLELSAEARAKLF
                     GYDRAGWQAEFESIGEFLDEYGPRMPAALKAEQVRISGALA"
     gene            845..3787
                     /locus_tag="PK818_00935"
     CDS             845..3787
                     /locus_tag="PK818_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY81332.1"
                     /translation="MQAQTNTAGAVTGRAASGDTITITNPATGFSRTITAGSDGSYRF
                     SALPTGQYQLSRNGGAPRNVTVNVGGAANIDFVTSGGDATTLDTVTVVGAGAINPIDV
                     SSVESTTILTAEQIAKIPVARDTTAVALLAPGTVRGDSAFGNLASFGGSSVAENQYYF
                     NGFNITNSFNNLAFAQIPFEAIAEQQTKTGGYGAEFGRSTGGVVNQISRRGTNEFMAG
                     GNLFYTPGSLRENFPNQVYNDGTVAADNSSDKFSDELTAAVWAGGALIKDRLFAYGLV
                     QYTKLDDNLSLPGGNGNGTSDVNTQPKWLLKMDWNISDNHILEFTAISDKREIETDYY
                     DVTATSGVPTVGAFKGTEYSEFGGNTYIGKYTGYLTDSFTLSVLAGTTESARGNYSVA
                     ANGTVNEYLGTVGVPANGCPVIVDARPSAVNNIVPRRVGCEIASVLGRPDAKDTRDQF
                     RIDAEWQLGAHLLRAGVDIDNFESVAGESYSGGIVWRYGKYSRTGITSENSVVRKRVF
                     QSGSTVAVDQTAFYIEDSWNVTDNFIAYLGLRWDNFENMNGDGETFAEIKNQFAPRLG
                     FSWDVYGDSTFKVYGNAGRYALPLTATVAVRGASKSLYSEQFYTYTGVDPVTGAPTGL
                     TLIPNYNNGRDIRYLNNEFGVGKNPETISDQGLKPMYQDEYILGFQKQITDTFSLGTR
                     LIHRDLKAAIDDQCDYRPIIDWALANGFTADGGIFIEPVDKTQNSDIAVYNPGFAFCH
                     LYNPGRDSVFKMDVNGDGVLETVELDADILGPKAKRKYTAFEVFFESSWDRAFLQGSY
                     TFAKSIGNTEGGVKSDIGQDDTGTTQDFDYPELAEGSYGYLPNDRRHSFKLFGSYDVT
                     ETVSIGGNFAAQSGRPINCFGYHPISPNYGNSYFYCDGVQVPRGTAGRLPWTQKLDLN
                     VSWSPAFADDHLTLKVDVFNVLNSRKAINVNEFGEDSGGTLQPNIYKSVNGWQTPRAV
                     RFMVQYDF"
     gene            3915..5492
                     /locus_tag="PK818_00940"
     CDS             3915..5492
                     /locus_tag="PK818_00940"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQY81333.1"
                     /translation="MEADAAARWEEAERLNAAGRYQHARPLYAGLGSERMYAPLAHLR
                     LSVLDQRAGDLRGATAQGLAAMSAAYGDPELLELLCKTLLPLGETRAALACATALAGM
                     PAPLQALAEVGKLMSDHMQPEAALTLLQRAVAMGLAGAPAVRYLLGLNLMYLGRLDEA
                     RSELERSLAGNPGFAPAHWALSKVGPAPARGARIDRLRRLLERTESGARDAPLLWYSL
                     FHELDGDDRPGEAWPALQTAMQLRRGQVQHDESAQDALFAAAGNALEWASGAGAGAAL
                     DAGPQPVFVVGMPRTGTTLIEQRLCAATDLASAGELRDLAVQMRWCAQQPGPFHPDAR
                     LLAAVLPSHLQDLGQRYLEHTQWRAQGASSYADKWPENYLAIGHILAAMPAARVLCVV
                     RDPMDTCFSNLKEWFASSYYYSYDMAETARQYARFRRLLDRVQALAHPRVASVGYEAF
                     VERPRDSIAAIAGALSLPARQEGGAIATHTIATASAVQARDAISRGHMGAWRRYSQQL
                     QPMLAELERCGVAASDA"
     gene            5489..7084
                     /locus_tag="PK818_00945"
     CDS             5489..7084
                     /locus_tag="PK818_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQY81334.1"
                     /translation="MTAGTDSPLLAMLRYAEPFAAGSDLASLRRLLAGIQVPVEQKAE
                     RLIEASYAEALAGRYRTARTAAMMAFACGARAPRLREQLAARLRTSNDVALLLELIDS
                     SGDPRVIPIPLLVAYAAQLSYVHLQERALQMLDEAKRGDDRYPPVLLARAQVLTYLGR
                     MQEARGDLEQCIAIAPQIGQAHWFLAHIGRATRDDNHVQRLRGILARPGLPAREVASF
                     AFALHKELDDLGEHDAAWTALEQACGAKRSLLKYDVADSRDLVDMLVASPVPPVLPRA
                     DDGAARVPVFIVGMHRSGTTLLEQLLDGSPQVHGVGELYDFTSAMRYATDHHCKGVID
                     RTIVERAGDVDFGEVGQRYLDGMAWRLGDERCFTDKLPSNFLNIGFICRALPQARILH
                     MVRDPVETCFSNLRELFSDANPYSYDQRELADYYLQYRRLMAHWHAAYPGRILDVDYA
                     ELTRDPAGVMRMVAAFCGIDYLDAMSDPRSSGRAVATASAVQVRDRVVRRDVPKWAPY
                     AKHLQPLVAALRQGGVEVPELPA"
     gene            7132..7707
                     /locus_tag="PK818_00950"
     CDS             7132..7707
                     /locus_tag="PK818_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQY81335.1"
                     /translation="MPTPTTESDTAGYDAGEDAVLVRRAAAADVAAFELLYRRHHRRV
                     HGVIVRLVGHAGARAEDLTQEAFVRAWQALPSFRFESAVSTWLHRLAVNTALMELRAR
                     RSRPQADDDEDALDGLSTPDTAGRAVLGRDLERAVATLPPRARAVLVLHDVEGWKHEE
                     IAEELGMAVGSSKAQLHRARGLLRTRIGEAA"
     gene            7704..8231
                     /locus_tag="PK818_00955"
     CDS             7704..8231
                     /locus_tag="PK818_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81336.1"
                     /translation="MNEIPAPIGEAELRMALRGLRQDADPGHDLWPGIAERITALPRR
                     QAQVARSRPRWPMSLATAAALLLAVGVSWKMQPPPSDVAGSGTSIAATAGRPAPLLQR
                     EADTLTVQYQAALRELQPQAAHAGWQPGLEALDRSAAEIREALRRDPGSRLLLQRLRD
                     TYTRRLALSRRALYA"
     gene            8243..9148
                     /locus_tag="PK818_00960"
     CDS             8243..9148
                     /locus_tag="PK818_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HQY81337.1"
                     /translation="MSLSLKASILALAPILIITSATCPAFAATPIDQTRPLDPRGRVE
                     IDNLKGRVEVRAWDRPEVRITGSLGAGVEKFTVEGDEGALRIEVKYPNRDRNSEPTML
                     LVQVPLQAGLEVSTVSADIDVHGVAPRELSLESVSGDISANGAPRRASVESVSGDLVL
                     TFNSGDVEASAVSGDLTLGGRLSGQVALETVSGNMRVDSKGERLRRLSVETVSGDADL
                     KVALLPDGEISMESVSGDLALAVPRDLSAQVTGESFSGDLSAPDAKIQREEFGPGSSF
                     HARYGAGKGEVRIETFSGDATLRLL"
     gene            complement(9241..10065)
                     /locus_tag="PK818_00965"
     CDS             complement(9241..10065)
                     /locus_tag="PK818_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008572079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81338.1"
                     /translation="MLALALASGGAQAQSRAKRDAAVVPVVNKESGKLEAVLLLEPAT
                     TSTGAGARWRFGSTTLEATYGVQAGDSLALVCDRQNGLSSGIAQLADNCVMATLNDDG
                     AGGVSRHGSAGASISRGGNKLGMAIGNGRDSLPAWLAPGQRAGKVESSDFSLLAEKNI
                     GREAFVSVGGTVARARLVPAADVPQLVDQWNTRSLSIGGGFGAFGANIVGRVVNVPGE
                     SNVWKGLGLGLTWRTPWSGQLTLGADNVVTRGKNPFSTANQDDQGTIPYVRYKQDL"
     gene            complement(10304..11161)
                     /locus_tag="PK818_00970"
                     /pseudo
     CDS             complement(10304..11161)
                     /locus_tag="PK818_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399823.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
     gene            11284..12048
                     /gene="mtgA"
                     /locus_tag="PK818_00975"
     CDS             11284..12048
                     /gene="mtgA"
                     /locus_tag="PK818_00975"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HQY81339.1"
                     /translation="MSAPGKSRRGRRWRGWLWKLPLAFVAFSVLQVLALRFIDPPFSM
                     FMAARQLDAAIAGDFGFRIAHDWRGMDRISPNLPLALVASEDQNFASHHGFDFAAIEK
                     AQAHNEKMIERAEKRGKPVRRLRGASTISQQTAKNLFLWQGSGVTRWARKGLEVWYTA
                     LIETLWPKRRILEVYANIAEFGDGIYGAQAAARSFFRKDAAKLSAAEAARLAAVLPSP
                     KRYNAARPGPYVQRRTNSIQRQMRYMGGSGWLRTLD"
     gene            12083..13123
                     /locus_tag="PK818_00980"
     CDS             12083..13123
                     /locus_tag="PK818_00980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY81340.1"
                     /translation="MHRIAPEDRCLTLVIAAYNEADALPALQPRIAAALDAAEREGLQ
                     ARVLYIDDGSRDATWSILHGLAARDPRIGLLRLSRNFGKEAALTAGLDRIERGAALIL
                     DADGQDPPELLPRFIAKWCEGFDDVHGTRIERGGEGWLKRVTAHAFYRVMAALSKTPV
                     PRDTGDFRLLSPRALAALRQLRERHRFMKGLFGWIGFNQVAIPYHRQARVAGRSKFGF
                     WKLWNFALEGITSFSTAPLRAATYLGVATAFAAFVYAAWIVAKALLWGDPVAGWPSMM
                     AVILFLGGTQLVALGLIGEYLGRLYEESKQRPLYLVDCWQPATGVSSPQHSNPSGASH
                     AHGAATAGNEVG"
     gene            13083..13511
                     /locus_tag="PK818_00985"
     CDS             13083..13511
                     /locus_tag="PK818_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014090742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQY81341.1"
                     /translation="MRTVRQLLETKSAEVFSIAPEAPVIDAVRLMAERRIGALLVMEG
                     TRLAGILSERDYARKVVLQGRSSKDTPVRDIMTAQVISVGPQDSADHCMQLVTDSRIR
                     HLPVLEGGQVIGVVSIGDLVKAVIEEQRQELDHLQRYIAS"
     gene            complement(13515..15119)
                     /locus_tag="PK818_00990"
     CDS             complement(13515..15119)
                     /locus_tag="PK818_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HQY81342.1"
                     /translation="MSEVIFEAARRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGSV
                     KGRKAARHATSDWMALEKERGISVTSSVMQFPYEGRIVNLLDTPGHADFGEDTYRVLT
                     AVDSALMVIDVAKGVEERTIKLMEVCRLRDTPIMTFVNKLDREGKDPIELLDEIESVL
                     GIQCAPVTWPIGMGQRLKGVVHLVTGEVHLYEPGRNFTRQDSTIFPSLDDPRVAERIG
                     ADMLAELREQLELVQGASHPFNREAYLEGMQSPVFFGSGVNNFGVQPLLDFFVEHAPS
                     PQARATTTRLVEATEPRLTGFVFKIQANMDPMHRDRVAFMRVCSGKFAPGMKVLQPRT
                     GKELKLANALTFMASDRELAESAYPGDVIGIHNHGTISIGDTFTEGEALGFTGIPNFA
                     PELFRRARLRDPLKLKQLQKGLAQLSEEGATQFFRPLMSNDLILGAVGVLQFDVVAYR
                     LKDEYGVEAGFEQVQVATARWIRCADAKKLEEFREKNAMNLALDAAGHLVYLAPTRVN
                     LQLAQERAPAVEFLATREHAQAVEVG"
     gene            15146..17596
                     /locus_tag="PK818_00995"
     CDS             15146..17596
                     /locus_tag="PK818_00995"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQY81343.1"
                     /translation="MAAAKGISTDMQDDATHALDEQAQVAAGLPETIGPYRILGLLGE
                     GGMGRVYLARESHPPREVALKVVRGVSSAALDRFRREIEILGQLEHPGIVRLYAAGED
                     RIGGLPSPWFALEVVRGPDLRGYLEQAHPDLRARIGLLSQLARAVDFAHQRGIVHRDL
                     KPSNVLVDAHGQPKILDFGIARLHGDAGDGMTQAGQVMGTLPYMSPEQLAGQGHEADA
                     RSDVYALGAIGYELLSGKLPHPRLSSSTLFEAVDIVRNEDPAPLGRLNREARGDLSLV
                     VMKALAAEPGRRYPSAAAFADDLDAVLASRPVRAHAPTLAYRSARFVRRHRALSIAAG
                     IVFAALLAATIVSTLAAQRARAALAEAQARANELAAVNGFVETMLTEADPEHAQGRDL
                     RVRDILDVASAELKSSTLAPGATARLHQVLGVTWLGLGEGRRSRAAFEDALNLEKAPS
                     ALRDELLLGRARSAIASGDYDAGEKGLAGLRARRAQLEPTLRIAMEESAAELLRETGK
                     QKEAVLALRELLPQARKSLGPDAQRTLGLQLQLASALQLDGDYEAALFESRDAVERHA
                     RVFGERHPQTLYAWNQLGVVENKLGNAESAERAFRRAAEGRLTVLGDKHPATVISQFN
                     LGSFLIEHKQPGEGVPLVRQASAWLDANRPEGDAKALVARSVLAYGLEDQGDLVGAER
                     LLRSMLASQERLGGPGAPDTFAPRNNLGMLLMKRGRLQEALQEFETLDAQVRARLGAD
                     HPFAAIFASNRGECLGKLGRFDDARRVLEDSLVRLKAKFGNAHERTLVAARRLEFVYQ
                     NLNMSRESLALKALYKIE"
     gene            18068..18742
                     /locus_tag="PK818_01000"
     CDS             18068..18742
                     /locus_tag="PK818_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY81344.1"
                     /translation="MVPLVLIIGRWRKCHRALATCEARLRVVVAEAREQSAAQERERI
                     YSDLHDDLGAKLLALVYGAESPVQADLARAILQDLRDVVTRSRGTPGTLGDVLADIHA
                     EASQRLATVGIGLSWDAADDLPDPVLDSGHALHLHRIVREAISNTIRHAQARHLRVRV
                     RVRGGELLLDLTDDGSGVPAVVSAGSGMRNMRSRAGELDGRIDWAPGTEGGAKVLLRM
                     PLEAGA"
     gene            18739..19392
                     /locus_tag="PK818_01005"
     CDS             18739..19392
                     /locus_tag="PK818_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQY81345.1"
                     /translation="MSVPGGYASALVVDDLPGARAWLAAALQAAFPGIEVATAGTLGE
                     ALARIDPPPALALVDLGLPDGSGMQLIEALRTRGAATLCIVATVFDDDAHLFPALRAG
                     AQGYVLKDRDRDSLAGMLRGIAAGQPPLSPSIARRLLRHFQPLPAADADAPPLTPRET
                     EVLRLTAKGLTLNEVATALGLSRHTVSGYLKDIYRKLSVGTRAEAALEAARRGLVAP"
     gene            complement(19525..20424)
                     /locus_tag="PK818_01010"
     CDS             complement(19525..20424)
                     /locus_tag="PK818_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQY81346.1"
                     /translation="MTPRSHWWIVAGLAVALSAAHAGDKVYRWVDKDGRVHYGDRPPA
                     NATPMKVREIPVPVEPSAIARLRVEPGNGEYLVHADNTLAGPIEVLLGFARSSNVRGD
                     PPLPARATVPARGSALVARLRAQDPGRTGNFELSLRGVPGDPSARPQDVEYLLPLRQS
                     HARIDQGFGGRFSHTDEQNRHALDFAADIGTTVLAARAGTVMQVESDFSKAGLKQERY
                     GGRANFVRILHDDGSMAIYAHLKADGALVRVGQRVTAGQQIGLSGNTGFTTGPHLHFA
                     VQVNRGMRLVSIPFRMRGVRMTP"
     gene            complement(20421..21488)
                     /locus_tag="PK818_01015"
     CDS             complement(20421..21488)
                     /locus_tag="PK818_01015"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQY81347.1"
                     /translation="MQIRRLNTLRGIAALVVLVGHYSNRAQLWDAVLGTRAPQLGVML
                     FFLLSAFLMTVLYLDRPPTAPALRGYASARAARILPLFVAIVLLSWLAQQSGWPALMA
                     AAYDIPDLKSLASHLLTLYGVQVLWTIPAELHFYLVFAALWWLRPRLAWAIPLFSVVV
                     LGAFASGHWPLGPKQPVFGFPVDLPLLRGLPYFTAGMLIGIAYKRWQAPARLRHHGWV
                     LALPMLLLLYPAILERLTGWTYVMWFNPAILLAMSAIFAALVFLVPEGNPLLENRIGD
                     HLGKISYSLYLLHFPILLFMRGAGLASGGGGLALFLAATILAASATYHVYEFPMRRRI
                     RALAVQPGPAAEAVATGAGRA"
     regulatory      21614..21745
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            21903..22937
                     /locus_tag="PK818_01020"
     CDS             21903..22937
                     /locus_tag="PK818_01020"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF006449.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HQY81348.1"
                     /translation="MRAAQAVGPDVGNVRIGSIDAVLATRHAGTRKLRLAYELVGPAG
                     APVVVVAGGISAHRHVAANAVDASSGWAEGLTGAGRAIDPSRQQLLAFDYVGACGGID
                     APIDTADQADAVALLLDALGIDALQAFVGYSYGALVGLQLAARHPRRLHKLVAVSGAH
                     RPHPYASAWRALQRKAVALGQLQCADEQGLSLARQFAMLSYRTPEEFGERFDAAPTLE
                     NGHVRVAAEDYLEAAGAKFVARIPATAWLRLSESIDLHQVDPTLIEVPTLVVAVEGDR
                     LVPLSDAVGLVEGLGLNGRLRVLRSQYGHDAFLKETDRIDALLAAELGASGVRDAVPA
                     IPHDFIGASV"
     gene            22934..24145
                     /locus_tag="PK818_01025"
     CDS             22934..24145
                     /locus_tag="PK818_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine (thiol)-lyase"
                     /protein_id="HQY81349.1"
                     /translation="MSQVQSVSGARRATRSVRAGIDRDTAFGAVTPPLVLSSNFSFAG
                     FAEKRQYDYTRSGNPTRDLLGEALAELEGGAGGVVTSTGMSAITLVLNALLQPGDTLV
                     VPHDCYGGSWRLFNALAKKNAFKLVTCDLTDPRALAEALEASPALVWIETPSNPLLRI
                     TDLRFVIEAAHKAGALAVVDNTFLSPALQRPIADFDADVVVHSTTKYINGHSDVVGGA
                     VIAREQAQHELLAWWANCLGLTGAPFDSFLTLRGLRTLDARLRVHQENTQALVALLDG
                     HPAVRALHYPGLASHPGHALAARQQHGFGAMLSVELEGGEAEVRAFIDGLQYFTLAES
                     LGGVESLVAHPATMTHAAMSAEARAAAGISDGLLRLSVGIEHVDDLVADIQAALQRAQ
                     DAAGCPRKVAR"
     gene            24142..25230
                     /locus_tag="PK818_01030"
     CDS             24142..25230
                     /locus_tag="PK818_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HQY81350.1"
                     /translation="MNARVAQLRGHAAQPARVALLGTGTVGSAAWARLASWAGTPLGE
                     RLSLVHVANSRVAISDRNGITADTRELLLHNAPRESSLAAVADALAGTGTRIVVDATA
                     SEAVADQHPRWLASGIHVVTACKLGQGTSLARWRAIQFARARGGSDYGDSATVGAGLP
                     LLRTLRELQAGGDRIHAIAGVLSGSLAWLFNHYDGLRPFSGFVRAAREAGYTEPDPRD
                     DLSGEDVRRKLLILSRAAGHALEADAVQVDSLVPPVLAALAGADVDAALQCLDAPLRE
                     RYAAAYKNGEKLRFIARLQAGGLATVGLEALPAAHPLAGGAGNDNRVAIWSDRYREQP
                     LVIQGPGAGAEVTAAALLDDALRIARGR"
     gene            complement(25227..27428)
                     /locus_tag="PK818_01035"
     CDS             complement(25227..27428)
                     /locus_tag="PK818_01035"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQY81351.1"
                     /translation="MQARLASVLRGALLTGLYFAGAVIAVFYLRTPADVTLFWPAAGI
                     GLAAVLRYGLWQTLTIPVAMLLLHLFVVPVPPVFLGYSLASNTLATLLAGWYVRRGHD
                     RNRRIHLRTSDGFLLLRGGLLLSVVSAGIGSVGMLHAGMAPAGDIVRLFLQWTLGDLL
                     GVSTLGPLLLYLFYRKQGSPARKESRRLELLAWSLTVALVLAMVFLVGNLGTQYPLAL
                     ASLPLGLLIFSAVRYTPLVTLLGTTIVIVCVSLMTGLGLGGLERPQTMHDSVLMMSML
                     VMYSIIPVLLLASRHEHAAATAALHQRATRDTLTGLLNRDAFEEQARAQLAAGTGTLT
                     LLYADLDHFKLVNDSASHVAGDEMIRGVAALFREEFGEDTLIARTGGDEFAVLATIDE
                     RAASTRARRLLSAIERMRVAWLGQNLGTTASIGLATSEPPHLAFDDLLSQTDAACFAA
                     KELGGNRSFAAASNPDELRQRTRAMHSAMAAREALDQRRFVLWCQPIVDLRNPAPPQS
                     HFEVLVRWMDTDGNLRPPAELVAAAERYRLGPRLDRFVLDATLGWLEQHPEATAQVEQ
                     CCINIGGTTLVDEDFGDFVAARLRRSPLRAEQLCLEITETSVVRDMTRARHFIGRMRD
                     LGCRFALDDFGTGFCSFGYLRDLDVDYLKIDGSFVRDLDDIHDEGGSLSEAVVRSITE
                     IAHVLGKRAVAEQAETELQLQRLREIGVDYAQGYVYQRPRPIAAFFAGDLG"
     gene            complement(27485..>27676)
                     /locus_tag="PK818_01040"
     CDS             complement(27485..>27676)
                     /locus_tag="PK818_01040"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HQY81352.1"
                     /translation="CIERNAMGAVKAINASRMALRGDGKHKVSLDKVIRTMRDTGRDM
                     QDKYKETSRGGLAVNVIEC"
BASE COUNT         4319 a         9398 c         9709 g         4250 t
ORIGIN      
        1 gcgacccgat ggcgatgaag ccgttctgcg gctacaactt cgccgactat ttcgcgcact
       61 ggctgtcgtt cgacaccgag ggcgcgaagc tgccgaaggt cttccacgtc aactggttcc
      121 gcaagggcga cgacggcaag ttcctgtggc ccggcttcgg cgacaacctg cgcgtgctgg
      181 agtggatgat ccggcgcgtc gaaggcgggg caggtgcggt cgaaaccccg atcggccgcc
      241 tgccggccga agccgacctc aatctggaag gactcgaact gtccgccgaa gcccgcgcca
      301 agctgttcgg ctacgaccgt gccggctggc aggcggagtt cgagagcatc ggtgagttcc
      361 tggacgagta cggcccgcgc atgccggcgg cgctgaaggc ggagcaggtg cggatttccg
      421 gcgcattggc ctgacgaagg tggtgctgca tcgcagcatg atccggcctt gcgccgtcgg
      481 cagaggaacg agcctgcacc tttgaccgga tcgacgcgac aaggccaagc ctgcgtgcgc
      541 ccgttcggaa tgtcgccagt tgcacgccgc cgggcaatcc ggcggtccga tgctgcactg
      601 cggcattttg acgcggcgcg aaaaattccg cgccccgcaa tgtgaatggg acgacaaggg
      661 cttgttaagg attcgttggc tggctatagt cggcgagtcg ggccggtcca ggccgggcgt
      721 acatgtacat ctctggtcac aactttccct atgggggtta cacaacatgg cttctaacta
      781 ctttgccaaa ggcctgaagc ggagcgcgct taccgtcgct ctgggtctgt gctttgtcgg
      841 cggcgtgcag gcccagacga ataccgccgg tgcagtcacc ggccgtgccg cctctggcga
      901 caccatcacc atcaccaacc cggccaccgg cttcagccgc accatcaccg ctggcagcga
      961 tggcagctac cgcttctccg ccctgcccac cggccagtac cagctgagcc gcaacggcgg
     1021 tgccccgcgc aacgtcacgg tcaacgtcgg cggcgcggcc aacatcgact tcgtgacctc
     1081 tggcggcgat gccaccaccc tcgacaccgt gaccgtcgtc ggtgccggcg cgatcaaccc
     1141 gatcgacgtc tcctcggtcg aatcgaccac catcctgacc gccgagcaga tcgcgaagat
     1201 cccggtcgcg cgtgacacca ccgcggtggc cctgctggcc ccgggtaccg tgcgcggcga
     1261 ctcggcgttc ggcaacctgg cgtccttcgg tggctcgtcg gtggccgaga accagtacta
     1321 cttcaacggc ttcaacatca ccaacagctt caacaacctc gccttcgcgc agatcccgtt
     1381 cgaagcgatc gccgagcagc agaccaagac cggcggctac ggcgcggaat tcggccgttc
     1441 gaccggtggc gtcgtcaacc agatctcgcg tcgcggcacc aacgagttca tggccggtgg
     1501 gaacctgttc tacaccccgg gcagcctgcg cgagaacttc ccgaaccagg tttacaacga
     1561 cggcaccgtt gcggctgata attcgtccga caagttctcc gacgagctga ccgctgcggt
     1621 ctgggccggt ggcgcgctga tcaaggaccg cctgttcgcc tacggcctgg tgcagtacac
     1681 caagctggac gacaacctgt cgctgcccgg tgggaacggc aacggtacct ccgacgtcaa
     1741 cacgcagccg aagtggctgc tgaagatgga ctggaacatc agcgacaacc acatcctcga
     1801 attcaccgcg atttccgaca agcgtgagat cgagaccgac tactacgacg tgaccgccac
     1861 cagcggcgtt ccgacggtgg gtgcgttcaa gggcaccgag tacagcgaat tcggtggcaa
     1921 tacctacatc ggcaagtaca ccggctacct gaccgacagc ttcaccctgt cggtgctggc
     1981 cggtaccacc gagtccgccc gcggcaacta ctccgtggcc gccaacggca cggtcaacga
     2041 gtacctgggt acggttggcg tgcccgccaa cggttgcccg gtcatcgtcg atgcgcgtcc
     2101 gtccgccgtg aacaatatcg ttccgcgccg cgttggctgc gagatcgcca gcgtcctcgg
     2161 tcgtccggac gccaaggaca cccgcgacca gttccgcatc gacgcggaat ggcagctggg
     2221 tgcccacctg ctgcgcgccg gcgtcgacat cgacaacttc gagtcggttg caggtgagtc
     2281 gtactccggc ggcatcgtct ggcgttacgg caagtacagc cgcaccggca tcaccagcga
     2341 aaattcggtc gtgcgcaagc gggtgttcca gtccggttcc accgtcgccg tcgaccagac
     2401 cgcgttctac atcgaagaca gctggaacgt caccgacaac ttcatcgcct atctcggcct
     2461 gcgctgggac aacttcgaga acatgaatgg tgacggcgaa accttcgccg aaatcaagaa
     2521 ccagttcgcc ccgcgcctgg gcttcagctg ggacgtgtac ggtgattcca ccttcaaggt
     2581 gtacggcaac gccggccgtt acgcgctgcc gctgaccgcc accgtggcgg tgcgtggtgc
     2641 ctcgaagtcg ctgtacagcg agcagttcta cacctacacc ggcgtggatc cggtgaccgg
     2701 ggcgccgacc ggcctgaccc tgatcccgaa ctacaacaac ggtcgcgaca tccgctacct
     2761 gaacaacgag ttcggcgtcg gcaagaaccc ggagaccatc tccgaccagg gcctgaagcc
     2821 gatgtaccag gacgagtaca tcctgggctt ccagaagcag atcaccgata ccttctcgct
     2881 gggtacccga ctgatccatc gcgacctgaa ggcggcgatc gacgaccagt gcgactaccg
     2941 tccgatcatc gactgggcgc tggcgaacgg cttcaccgcc gacggcggca tcttcatcga
     3001 gccggtcgac aagacccaga acagcgacat cgccgtctac aaccccggct ttgcgttctg
     3061 ccacctgtac aacccgggcc gcgactccgt cttcaagatg gacgtcaatg gcgacggcgt
     3121 cctggagacg gtcgagctcg acgccgacat cctgggcccg aaggccaagc gcaagtacac
     3181 cgcgttcgaa gtgttcttcg aaagcagctg ggaccgcgcg ttcctgcagg gttcgtacac
     3241 cttcgccaag agcatcggca acaccgaagg tggcgtgaag tcggatatcg gccaggacga
     3301 taccggcacc acccaggact tcgactaccc ggaactggct gaaggttcgt acggctacct
     3361 gccgaacgac cgtcgccaca gcttcaagct gttcggtagc tacgacgtga ccgaaaccgt
     3421 gtcgatcggc ggcaacttcg cggcgcagtc cgggcgtccg atcaactgct tcggttacca
     3481 cccgatctcg ccgaactacg gcaacagcta cttctactgc gatggcgtcc aggtgccgcg
     3541 cggtaccgct ggtcgcctgc cgtggaccca gaagctggac ctgaacgtgt cgtggtcgcc
     3601 ggccttcgcg gacgatcacc tgaccctgaa ggtcgacgtg ttcaacgtgc tgaacagccg
     3661 caaggcgatc aacgtcaacg agttcggtga agactcgggc ggcaccctgc agcccaacat
     3721 ctacaagtcg gtcaacggct ggcagacccc gcgtgcggtg cgcttcatgg tgcagtacga
     3781 cttctgatag ccaagcaatt gttgtgcttc ggacggccgc cttgtgcggc cgtccttttt
     3841 tgcctggacc ggcgggcgag gttaaagtcc gtggcggttt gcgcgtgggc gtgggcaacg
     3901 ggaatggaga gtcgatggaa gcggacgccg ccgcgcgttg ggaagaagcc gaacggctga
     3961 acgcggcggg gcggtaccag cacgcgcgtc cgttgtacgc cgggctggga agcgagcgca
     4021 tgtatgcgcc gctcgcgcac ctgaggctgt cggtgctgga ccaacgcgcc ggcgacctgc
     4081 ggggtgcaac cgcgcagggc ctggccgcga tgtcggccgc atacggcgat ccggaattgc
     4141 tggaattgct gtgcaagacc ctgttgccgc tcggcgagac ccgcgcggcg ctcgcctgcg
     4201 ccacggcgct cgcgggcatg ccagcgccgc tgcaggcgtt ggcggaagtg ggcaaattga
     4261 tgtccgacca catgcagcca gaggcggcgt tgaccctgct gcagcgcgcg gtggcgatgg
     4321 ggctcgccgg tgcgccggcg gtgcgctatt tgctgggact gaacctgatg tacctcggcc
     4381 gcctcgacga agcgcggtcg gaactggaac gcagcctggc gggcaacccg gggttcgccc
     4441 ccgcccactg ggcgctgagc aaggtggggc ctgcccctgc gaggggtgcc cggatcgacc
     4501 gcctgcgccg gctgctggag cgcaccgaat cgggcgctcg cgacgcaccg ctgctctggt
     4561 acagcctgtt ccacgaactg gatggtgatg accggccagg cgaggcatgg cccgcgctgc
     4621 agactgcgat gcagttgcgc cgcggccagg tccagcatga cgaatcggcg caggacgcgc
     4681 tgttcgcggc ggccgggaac gccctcgaat gggccagcgg cgcaggtgca ggcgccgcgc
     4741 tggacgcggg gccacagccg gtgttcgtgg tcggcatgcc gcgaaccggc accaccctca
     4801 tcgagcagcg cctgtgtgca gccaccgacc ttgccagtgc cggcgagctg cgggacctgg
     4861 cggtgcagat gcgctggtgc gcgcagcagc ccgggccatt ccacccggac gcaaggttgc
     4921 tcgcggcggt cctgccgtcg cacctgcagg acctcggcca gcgctacctg gaacacaccc
     4981 agtggcgggc gcagggggca tcctcctatg ccgacaaatg gccggaaaac tacctggcga
     5041 tcggccacat ccttgccgcg atgccagcgg ccagggtcct ttgcgtggtc cgcgatccga
     5101 tggatacctg cttctcgaac ctcaaggagt ggttcgcgtc ttcctactac tacagctacg
     5161 acatggcgga aaccgcgcgc cagtacgcgc gcttccggcg cctgctggat cgcgtgcagg
     5221 cgctcgcgca tccgcgcgtg gcgagcgtgg gttacgaagc gttcgtggaa aggccgcggg
     5281 attcgatcgc ggccatcgcc ggggcgctct cgctgccggc acgccaagag ggcggtgcga
     5341 tcgcgacgca cacgatcgcc accgccagtg cggtgcaggc gcgcgacgcc atcagtcgcg
     5401 gccacatggg cgcgtggcgg cgctattcgc agcagctgca gcccatgctg gccgaactgg
     5461 agcggtgcgg cgtggcggcc tccgacgcat gaccgccggg accgacagtc cgctgctggc
     5521 gatgctcagg tacgcggagc cgttcgccgc aggcagcgac ctcgcgtccc tgcgccggct
     5581 cctggctggc atccaggtcc cggtggagca gaaggcggaa cgcctgatcg aggcttccta
     5641 tgccgaggcg ctggccgggc gctaccggac tgcgcggacg gcggcgatga tggcgttcgc
     5701 ctgcggcgcc cgcgcgccgc gcctgcgcga gcagcttgcg gcgaggttgc ggacaagcaa
     5761 cgacgtggcg ctgctgctgg aactgatcga ttcctccggc gatccccgcg tgatcccgat
     5821 ccccttgctg gtggcctatg cggcccagct ctcgtacgtg cacctgcagg agcgcgccct
     5881 gcaaatgctg gacgaggcca agcggggcga cgacaggtat ccgccggtgc tgctggcacg
     5941 cgcgcaggtg ctgacctacc tcggccggat gcaggaagcg cgcggcgacc tggagcaatg
     6001 catcgccatc gcgccgcaga tcggccaggc gcactggttc ctggcccaca tcggccgtgc
     6061 gacccgcgac gacaaccacg tccagcggct tcgcgggatc ctcgcgcggc ccgggttgcc
     6121 ggcgcgcgag gtggcgtcgt tcgcgttcgc gctgcacaag gaactcgacg acctgggcga
     6181 gcacgatgcc gcctggaccg ccctggaaca ggcgtgcggt gccaagcgca gcctgctgaa
     6241 gtacgacgtc gccgacagtc gcgacctggt cgacatgctg gtcgcaagtc cggtcccgcc
     6301 ggtgctgccg cgcgcggacg acggggctgc gcgcgtgccg gtcttcatcg tcggcatgca
     6361 ccgctccggc accacgctgc tggaacagct cctggatggc agcccgcagg tgcatggggt
     6421 cggcgagctt tacgacttca ccagtgcgat gcgctacgcc accgaccacc actgcaaggg
     6481 cgtgatcgac cgcaccatcg tggaacgcgc gggggacgtc gatttcggcg aggtcggcca
     6541 gcgctacctg gatggcatgg catggcggct cggcgacgag cgctgcttca ccgacaagct
     6601 gccgtccaac ttcctcaaca tcggcttcat ctgccgcgcc ctgccgcagg cgcggatcct
     6661 gcacatggtg cgcgatccgg tggagacctg cttctccaac ctgcgcgagc tgttttccga
     6721 cgccaacccg tattcctacg accagcgcga gcttgccgac tactacctgc agtaccggcg
     6781 gctgatggcg cattggcacg ccgcatatcc gggccggatc ctggacgtgg actacgcgga
     6841 gctgacccgc gatccggccg gcgtgatgcg catggtcgcg gcgttctgcg gcatcgacta
     6901 cctcgatgcg atgagcgatc cgcgcagcag cgggcgcgcg gtcgccaccg ccagcgcggt
     6961 gcaggtgcgc gaccgggtgg tgcgccggga cgtgccgaag tgggcgccct atgcaaagca
     7021 cctgcagccg ctggtcgcgg ccctccggca gggcggggtc gaggttccgg agctgccggc
     7081 ttaaaccctc cgccgcggcg ccgcatcgga ctgggtatgc tgatgcaggc catgccgacg
     7141 ccaaccaccg agagcgacac cgccggctac gacgcgggcg aggacgccgt gctggtgcgg
     7201 cgcgcggccg cggcggacgt ggctgccttc gagttgctgt accgccgcca ccaccggcgc
     7261 gtgcacgggg tgatcgtgcg cctggtcggg cacgccggcg cgcgcgcgga ggacctcacc
     7321 caggaggcgt tcgtgcgcgc atggcaggcg ctgccgtcgt tccgcttcga aagcgcggtc
     7381 tcgacctggc tgcaccggct tgcggtcaac accgcgctga tggagctgcg cgcccgccgt
     7441 agccggccgc aggcggacga tgacgaggac gcgctggacg ggttgtcgac gccggatacc
     7501 gccggtcgcg cggtgctcgg gcgcgacctg gagcgcgcgg tggcgaccct gccgccacgc
     7561 gcgcgcgcgg tgctggtgct gcacgacgtg gaaggctgga agcacgagga aatcgccgag
     7621 gaactcggca tggcggtggg cagctcgaag gcgcaattgc atcgcgcgcg cggcctgctg
     7681 cgcacgcgca taggagaagc ggcatgaacg agatccccgc cccgatcggt gaagccgagc
     7741 tgcgcatggc cttgcgtggg ctgcgccagg acgccgatcc cggccacgac ctgtggcccg
     7801 gtatcgccga acgcatcacg gcactgccgc gaaggcaagc gcaggttgcg cggtcacggc
     7861 cgcgttggcc gatgtcgctg gcgactgcgg ccgcgctgct gctggcggtc ggtgtgtcct
     7921 ggaagatgca gccgccgcca tcggacgtgg ccgggtccgg aacctcgatc gccgccaccg
     7981 ccggccggcc agcgccgctg ctgcagcgtg aagccgacac cctgaccgtg cagtaccagg
     8041 ctgcgctgcg cgaactgcag ccgcaggcag cgcacgccgg ctggcagccg ggcctggagg
     8101 cgctggaccg cagcgccgcc gagatccgcg aggccttgcg gcgcgatccc ggttcgcgcc
     8161 tgctgctgca gcggttgcgc gatacctaca cccgccggct cgccctgtcg cgccgcgcgc
     8221 tctacgcctg aaggagtccg atatgtccct gtccctgaaa gcctcgatcc tggccctggc
     8281 cccgatcttg atcattactt ccgcaacgtg cccggcattc gccgccaccc cgatcgacca
     8341 gacccggccg ctggatccgc gtggccgggt cgagatcgac aacctcaagg gccgcgtcga
     8401 agtgcgcgcc tgggaccggc cggaggtccg gatcaccggc agcctcggcg ccggggtcga
     8461 gaaattcacg gtggagggcg atgaaggcgc attgcggatc gaggtgaagt acccgaaccg
     8521 cgaccgcaac agcgagccga cgatgctgct ggtgcaggtg ccgttgcagg ccgggctgga
     8581 agtcagcacg gtgtcggcgg acatcgacgt gcacggcgtc gccccgcgcg agttgtcgct
     8641 ggagtcggtg agcggtgaca tcagtgcaaa cggggcgccg cgccgcgcgt cggtggaatc
     8701 ggtgagtggc gacctggtgc tgaccttcaa cagcggcgac gtcgaggcca gcgcggtcag
     8761 cggcgacctc accctcggcg gccggctcag cggccaggtc gcgctggaaa ccgtgtccgg
     8821 caacatgcgg gtggacagca agggcgagcg cctgcgcagg ctgtcggtgg agaccgtgtc
     8881 cggcgatgcc gacctgaagg tggcgctgct gccggatggc gagatcagca tggaaagcgt
     8941 cagcggcgac ctggccctgg ccgtcccccg cgacctctcc gcgcaggtga ctggcgaaag
     9001 tttcagtggc gacctcagcg cgcccgatgc gaagatccag cgcgaggaat tcggccccgg
     9061 ctccagcttc cacgcccgct atggcgcggg caagggcgag gtgcgcatcg aaaccttctc
     9121 cggcgacgca accctgcgcc tgctgtagcc gccgacgcga tccggatccc gcaaacacga
     9181 aggccgcatc gatgatgcgg cctttcgctt gtgcggacgt gtcgtcgaaa ccagccgggc
     9241 ctacaggtcc tgcttgtagc gcacgtaggg gatggtgccc tggtcgtcct ggttggcggt
     9301 cgagaacggg ttcttgccgc gggtgaccac gttgtccgcg cccagcgtga gctggccgct
     9361 ccacggggtg cgccaggtca ggcccaggcc caggcccttc cagacgttgc tctcgcccgg
     9421 cacgttgacc acccgtccga cgatgttggc gccgaaggca ccgaagccac cgccgatgct
     9481 gaggctgcgg gtattccact ggtccaccag ttgcggcacg tcggccgccg gcaccagccg
     9541 cgcacgggcg acggtgccgc cgacgctgac gaaggcctcg cggccgatgt tcttctcggc
     9601 gagcagcgag aaatcgctgg actcgacctt gcccgcgcgc tggccaggcg ccagccacgc
     9661 cggcagcgag tcgcggccgt tgccgatcgc catgccgagc ttgttgccac cgcggctgat
     9721 gctggcgcct gcgctgccgt ggcggctcac gccaccggcg ccatcgtcgt tcagggtggc
     9781 catcacgcag ttgtcggcga gttgggcgat gcccgaggac agaccgttct ggcggtcgca
     9841 cacgagcgcc agcgagtcgc cggcctgcac gccataggtc gcttcaaggg tggtgctgcc
     9901 gaagcgccag cgtgcgccgg cgccggtact ggtggtggcg ggctccagca ggagcacggc
     9961 ttccagcttg ccgctttcct tgttcaccac cggcaccact gcggcgtcgc gcttggccct
    10021 ggattgcgcc tgcgcgccgc cgctggcgag ggccagggcg agcagtgcgg cggcaatggg
    10081 tgtcaggcgc atgcgcatgg tgctggatgg acaagccgcg agcggcgaag ttcctctccc
    10141 cggggctacc tagcctagcc gattcatgat tctttaacaa tgcgcgccag ggcagcccgg
    10201 ggccagcgcg gatatccaat cgcgcgaggc gtcactggat cccgggcgcg gcgggcagtg
    10261 cggcccccgc gccgcggaac aggctggcga ccgcttcggc gtcgaaccgg tagtcatggc
    10321 cgcagaactc gcacagcacc cgcgcctcgc cgtcgccggc ggcgaccgcg gcttcggcct
    10381 cgccgatgcc cagcgagacc agcatcgccg ccacccgctc gcgcgagcac gaacaggcga
    10441 accgcagcgg cttgccgccc attgcgtgcg gtgattcctc gtggaacagg cggtggatgg
    10501 tgtcggcggg cgccagcgcc agcagttccg cgctgccgag ggtctcgaac agcgcgccga
    10561 cccggttcca gccgtcgtcg tcgccgccgt cgccgggcag cttttgcaac atcaggccgg
    10621 ccgcgcgcgc gccgtcgcag gccagcagca ggcgggtcgg cagctgctcg gactggcgga
    10681 agtagccctc gaaggcttcg ccgaggttgt ccgcgtcgag ttcgaccagg ccctggtagc
    10741 gcatcggctc gcggccgccc agcgcgggat tctcgatggt gatggccagc accgcgtcgt
    10801 tgccgagcga acgcaggtcg cgcgatgggg tcgccgcgcc gggttcggac gcatcctcct
    10861 gcagccgcgc gatcccgcgc agggtgccgg ccgcggtgca ttcggcgaac agggtgcgca
    10921 gggcgccgtg gctgcgcagt tgcaccgaga gccggccgtc gaccttggca tgcccggtga
    10981 acagcgcggc cgcggcggcc gcttcgccca gcagttcggc gaccgcgggc ggatacgccg
    11041 cgcgcgcggc catcgcccgc caggtctggt ccagctgcac gagcaccccg cgcacgccgg
    11101 cgctgggcag caggaaccgc agttgcaggt cggacggggc gggcgcgcgg gcttgggtca
    11161 tgtcgatacc ggaattcgcc cggcctgcca aaatgcacgg ggcgtgccgt cggcaggcga
    11221 tcggggcaag ggagtggcga aggatatggg gtacgcgggt acgggacaca aggcgatcca
    11281 cccatgagcg caccgggcaa gtcgcggcgc ggccggcgct ggcgcggctg gttgtggaag
    11341 ctgccgctgg cgttcgtcgc gttcagcgtg ttgcaggtgc tggcgctgcg cttcatcgac
    11401 ccgccgttct cgatgttcat ggccgcgcgc cagctggatg cggccatcgc cggcgatttc
    11461 gggttccgca tcgcccacga ctggcgcggc atggaccgga tctcgccgaa cctgccgctg
    11521 gcgctggtgg cttcggaaga ccagaacttc gcaagccacc atggcttcga tttcgccgcc
    11581 atcgaaaagg cgcaggcgca caacgaaaag atgatcgagc gcgcggagaa gcgcggcaaa
    11641 cctgttcgcc gcctgcgcgg tgccagcacg atcagccagc agaccgccaa gaacctgttc
    11701 ctgtggcagg gcagcggcgt gacccgctgg gcccgcaagg gactggaggt ctggtacacc
    11761 gcgctgatcg agaccctttg gccgaagcgg cggatcctgg aggtgtacgc caacatcgcc
    11821 gaattcggcg acggcatcta cggcgcgcag gcggccgcgc gcagcttctt ccgcaaggac
    11881 gcagcgaagc tttcggcggc cgaagcggcg cggctggcgg cggtgctgcc cagccccaag
    11941 cgctacaacg ccgcacgacc cggcccttac gtgcagcgcc gcaccaactc gatccagcgg
    12001 cagatgcgct acatgggcgg cagcggctgg ctgcgcacgc tggactgaac atcgccggcg
    12061 caagctggca taccatcgcc gcatgcaccg catcgccccg gaagaccgct gcctgaccct
    12121 ggtgatcgcc gcctataacg aggcggatgc gctgccggcc ctgcagccgc ggatcgccgc
    12181 cgcgctggat gcggccgaac gcgaaggcct gcaggcgcgg gtgctgtaca tcgacgacgg
    12241 cagccgcgat gccacctgga gcatcctgca cgggctggcc gcgagggatc cgcggatcgg
    12301 cctgctgcgg ctgtcgcgca acttcggcaa ggaggcggcg ctgaccgccg ggctggaccg
    12361 gatcgaacgc ggtgccgcgc tgatcctcga tgccgacggc caggatccgc cggaattgct
    12421 gccaaggttc atcgccaagt ggtgcgaggg gttcgacgac gtccacggca cccgcatcga
    12481 gcgcggcggc gaaggctggc tcaagcgcgt caccgcgcac gcgttctacc gggtgatggc
    12541 ggccctttcg aagaccccgg tcccgcgcga caccggcgac ttccgcctgc tgtcgccgcg
    12601 cgcgctggcg gcgctgcggc agctgcgcga gcgccaccgc ttcatgaagg gcctgttcgg
    12661 ctggatcggc ttcaaccagg tcgcgattcc ctaccaccgc caggcgcgcg tggccgggcg
    12721 cagcaagttc ggcttctgga agctgtggaa cttcgcgctg gaaggcatca ccagcttcag
    12781 caccgcgccg ctgcgcgcgg ccacctacct cggcgtggcc accgcgttcg cggccttcgt
    12841 gtacgccgcg tggatcgtcg ccaaggccct gctgtggggc gatccggtgg ccggctggcc
    12901 aagcatgatg gcggtgatcc tgttcctggg cggcacccag ctggtggcgc tggggttgat
    12961 cggcgaatac ctgggccggt tgtacgagga atccaagcag cggccgctgt acctggtcga
    13021 ctgctggcag cctgcgaccg gagtatcctc gccacagcat tcgaacccgt cgggagcaag
    13081 ccatgcgcac ggtgcggcaa ctgctggaaa cgaagtcggc tgaggtcttt tcgatcgccc
    13141 cggaagcacc ggtgatcgac gcggtgcggc tgatggcgga gcgccggatc ggcgcgttgc
    13201 tggtgatgga aggcacgcgc ctcgccggca tcctctcgga gcgggactac gcacgcaagg
    13261 tggtgctgca ggggcgttcg tcgaaggaca ccccggtgcg cgacatcatg accgcgcagg
    13321 tgatcagcgt cggcccgcag gattcggccg accactgcat gcagctggtg accgacagcc
    13381 gcatccgcca cctgccggtg ctcgaaggcg ggcaggtgat cggcgtggtg tcgatcggcg
    13441 acctggtgaa ggcggtgatc gaggaacagc ggcaggaact ggaccacctg cagcgctaca
    13501 tcgcgtcctg atcctcagcc gacctccacc gcctgcgcgt gttcgcgggt ggccaggaac
    13561 tcgaccgccg gcgcgcgttc ctgcgccagc tgcaggttga cccgggtcgg cgccaggtag
    13621 accaggtggc cggcggcgtc cagcgccagg ttcatcgcgt tcttctcgcg gaactcctcg
    13681 agcttcttcg cgtccgcgca acggatccag cgcgcggtgg ccacctgcac ctgctcgaag
    13741 ccggcctcga ccccgtattc gtccttcagc cggtacgcca ccacgtcgaa ctggagcacg
    13801 ccgaccgcgc ccaggatcag gtcgttgctc atcagcggcc ggaagaactg ggtcgcgcct
    13861 tcctcgctca gctgggccag gcccttctgc agctgcttga gcttgagcgg atcgcgcagg
    13921 cgcgcgcggc ggaacagttc cggcgcgaag ttggggatgc cggtgaagcc gagcgcctcg
    13981 ccttcggtga aggtgtcgcc gatcgagatg gtgccgtggt tgtggatgcc gatcacgtcg
    14041 ccggggtacg cgctctccgc cagttcgcgg tccgaggcca tgaaggtcag cgcgttggcc
    14101 agcttcagct ccttgccggt gcgcggctgc aggaccttca tgccgggcgc gaacttgccc
    14161 gagcacacgc gcatgaacgc cacccggtcg cggtgcatcg gatccatgtt ggcctggatc
    14221 ttgaacacga agccggtgag cctgggctcg gtcgcctcga ccaggcgggt ggtggtcgcg
    14281 cgcgcctgcg gcgagggcgc gtgctcgacg aagaaatcca gcagcggctg cacgccgaag
    14341 ttgttgacgc cggaaccgaa gaacaccggc gactgcatgc cctcgaggta ggcctcgcgg
    14401 ttgaacggat ggctggcgcc ctgcaccagc tccagctgct cgcgcagttc cgccagcatg
    14461 tccgcgccga tccgctccgc cacgcgcgga tcgtccagcg acgggaagat ggtggaatcc
    14521 tggcgggtga agttgcggcc gggttcgtac aggtgcactt cgccggtgac caggtggacc
    14581 acgcccttca ggcgctggcc catgccgatc ggccaggtga cgggcgcgca ctggatgccg
    14641 agcacggatt cgatctcgtc cagcagctcg atcgggtcct tgccctcgcg gtcgagcttg
    14701 ttgacgaagg tcatgatcgg ggtgtcgcgc aggcggcaca cctccatcag cttgatggtg
    14761 cgttcctcca cgcccttggc gacgtcgatc accatcagcg cggagtccac cgcggtgagc
    14821 acgcggtagg tgtcctcgcc gaagtcggcg tggccggggg tgtccagcag gttgacgatg
    14881 cggccctcgt acgggaactg catcaccgag ctggtcaccg agatgccgcg ctccttctcc
    14941 agcgccatcc agtcggaggt ggcgtggcgg gcggccttgc ggcccttgac cgaaccggcc
    15001 agctggatcg cgccgccgaa cagcagcagc ttttcggtga gcgtggtctt gccggcgtcg
    15061 gggtgggaaa tgatggcgaa ggtgcggcgg cgcgcggctt cgaagatgac ttctgacatt
    15121 gcggaattat agcggcgggg gcagcatggc cgctgccaag gggatcagca ccgacatgca
    15181 ggacgacgcc acccacgcgc tcgacgaaca ggcacaggtg gccgccgggt tgccggagac
    15241 gatcggtccc taccgcatcc tcggtttgct gggcgaaggc gggatgggcc gggtgtacct
    15301 ggcgcgcgaa tcgcatccgc cgcgcgaggt cgcgctgaag gtggtgcgcg gcgtctccag
    15361 cgccgcgctg gatcgcttcc gccgcgagat cgagatcctc ggccagctgg agcatcccgg
    15421 catcgtccgc ctgtacgccg ccggcgagga ccgcatcggc ggcttgccgt caccgtggtt
    15481 cgcgctggaa gtggtgcgcg ggcccgacct gcgcggctac ctggagcagg cgcatccgga
    15541 cctgcgcgcg cgcatcggcc tgctttcgca actggcgcgc gcggtggatt tcgcccacca
    15601 gcgcgggatc gtgcatcgcg acctgaagcc ctccaacgtg ctggtggacg cgcacgggca
    15661 gccgaagatc ctcgacttcg ggatcgcccg cctgcatggc gacgccggcg acggcatgac
    15721 ccaggccggg caggtgatgg gcaccctgcc gtacatgagc ccggagcaac tggccggcca
    15781 gggccacgag gccgacgccc gcagcgacgt gtacgcgctg ggcgcgatcg gctacgaact
    15841 gctgtccggc aagttgccgc acccgcggct gtcctcctcg accctgttcg aggcggtgga
    15901 catcgtccgc aacgaggacc cggcgccgct cgggcggctg aaccgcgagg cgcgcggcga
    15961 cctcagcctg gtggtgatga aggcgctggc cgccgagccc ggccgccgtt accccagcgc
    16021 cgccgcgttc gccgacgacc tcgatgcggt gctggcctcg cgcccggtgc gcgcgcacgc
    16081 acccaccctg gcctaccgca gcgcgcgctt cgtgcgccgc caccgcgcgc tcagcatcgc
    16141 cgccgggatc gtgttcgcgg cgctgctggc cgcgacaatc gtcagcacgc tggccgcgca
    16201 acgcgcgcgc gcggcgttgg cggaggcgca ggcccgcgcc aatgaactgg ccgcggtgaa
    16261 cggcttcgtc gaaaccatgc tgaccgaggc ggatccggaa catgcgcaag gccgggactt
    16321 gcgtgtcagg gacatcctgg acgtcgcctc ggctgaactg aagagttcga cgctggcgcc
    16381 gggtgcaacg gcacgcctgc atcaggtcct cggtgttacc tggctagggc tcggcgaggg
    16441 gcggcgttcc cgtgcggcct tcgaggacgc tttgaacttg gagaaggccc cgtctgccct
    16501 gcgggatgaa ctgctgctgg ggcgtgcgcg tagcgccatt gcctccggcg attacgacgc
    16561 cggggaaaag gggcttgcgg gtctgcgggc gcggcgtgcg caacttgaac cgacgctgcg
    16621 catcgcaatg gaggagtcgg cggcggagct cttgcgagaa accggcaagc agaaggaagc
    16681 ggtactggcg ttgcgcgagc tgcttcctca ggcacgcaag tcgctgggtc cggatgcgca
    16741 acggacgctc gggctgcagc tgcaactggc gtcggccttg caactcgatg gtgattacga
    16801 ggccgcgttg ttcgaaagcc gcgatgcggt cgaacgccat gcgcgggttt tcggtgagcg
    16861 gcatccgcag acgctgtacg cgtggaatca gcttggcgta gtcgagaaca agcttggcaa
    16921 cgccgagtcg gccgaacgcg cgttccgacg tgccgcagag gggcggctca cggtactggg
    16981 ggacaagcac ccggcgaccg tcatcagcca gttcaacctg ggatcattcc tcatcgagca
    17041 caagcagccc ggggaaggtg ttcccctggt gcgtcaagcc agtgcgtggc tggatgccaa
    17101 tcgcccggag ggagatgcca aggcgctagt tgcccgaagc gtgctggcgt atggactgga
    17161 ggaccaaggc gacctggttg gcgccgagcg cctgttgcgg agcatgctcg ccagccagga
    17221 gcgcttgggc ggtcctggcg cccctgatac gttcgcgccg cgcaacaacc tcgggatgct
    17281 cctgatgaag cgggggcggc tgcaggaggc gttgcaggaa ttcgagacac tggatgcgca
    17341 ggtacgcgca cgcctgggcg ctgatcatcc tttcgctgcg attttcgcca gcaatcgagg
    17401 cgaatgcctc ggcaagctcg gtcgcttcga tgatgcgcgg cgcgtcctgg aggacagtct
    17461 ggtgcgattg aaggcgaagt tcggcaacgc gcatgagcgg acactggtgg cggcgcgaag
    17521 gctggaattc gtctatcaaa accttaatat gagtcgcgag tcgttagcgt tgaaggcgtt
    17581 atacaagatt gagtagtgtc aggtcgcgac tcacgcaaat ccaatccgca aaaatccaac
    17641 ccatccgctt aacagttttt ggggaatatg aagaacaaaa tcattcttgc ttgtctgctc
    17701 gtcctgtctg cgccggacgc attcgctcag agtcaagtgt ctaccccgga ggggttttcg
    17761 attgactttt tcgagtgggt tgcttcgttg ttcgagagtc agtccgaaga ggaaaaccaa
    17821 gttgtcaagt cagacgggac tatttatgac acggctgtaa agtctgacgg cacagtttat
    17881 gacgccattg tcaagtcaga tggaatcatt tacgactaat tcttccttta aatagtgtcc
    17941 gcggactgaa attcggcctt ccgagttttg cgccatctgg ctgaattcca gcccgcattt
    18001 tcttgacaat actttgaatt ggccggtgtc gcacatgtgg agcgctgcgc tgacgacatt
    18061 ggtttttgtg gttccactcg tcttgataat tggtcgatgg cgcaagtgcc atcgggcgct
    18121 ggccacctgt gaggcccgcc tgcgggtggt cgttgccgag gcgcgcgaac agtccgccgc
    18181 gcaggaacgc gagcgcatct actccgacct ccacgacgac ctcggcgcga agctgctggc
    18241 gctggtgtac ggcgccgagt cgccggtgca ggccgacctt gcgcgggcga tcctgcagga
    18301 cctgcgcgac gtggtgacgc gctcgcgcgg gaccccgggg acgctcggcg acgtgcttgc
    18361 cgacatccat gccgaagcca gccagcggct ggcgacggtc ggcatcggcc tgtcgtggga
    18421 cgccgcggac gacctgccgg atccggtcct ggacagcggc cacgcgctgc acctgcaccg
    18481 gatcgtgcgc gaggcgatca gcaacaccat ccgccacgcc caggcgcgcc acctgcgggt
    18541 gcgggtgcgg gtgcgcggcg gcgagttgct gctggacctc accgacgatg gcagcggggt
    18601 gccggcggtg gtgtccgccg gcagcggcat gcgcaacatg cgcagccgcg ccggcgaact
    18661 cgacggccgc atcgactggg cgccgggcac cgagggcggg gccaaggtgc tgctgcggat
    18721 gccgctggag gcgggcgcgt gagcgtaccc ggcggttacg ccagcgcact ggtggtggac
    18781 gacctgccgg gcgcccgcgc gtggctggcg gccgcgctgc aggcagcatt cccgggaatc
    18841 gaggtcgcca ccgccggcac cctgggcgaa gcgctggcgc ggatcgatcc gccgccggcg
    18901 ctggcgctgg tcgaccttgg cctgccggac ggcagcggca tgcagctgat cgaggcgttg
    18961 cgcacgcgtg gcgcggcgac cctgtgcatc gtcgccaccg tgttcgatga cgacgcccac
    19021 ctgttcccgg cattgcgggc cggtgcgcag ggctacgtgc tgaaggaccg cgaccgcgac
    19081 agcctggccg gcatgttgcg cggcatcgcc gccggccagc cgccgttgtc gccgtcgatc
    19141 gcgcggcgcc tgctgcgcca tttccagccg ctgccggcgg cggatgcgga tgcgccgcca
    19201 ctgaccccgc gcgaaaccga ggtgctgcgg ctgaccgcca aggggctgac cctgaacgag
    19261 gtggccacgg cgctggggct ttcgcgccac accgtgtccg gctacctcaa ggacatctac
    19321 cgcaagctga gcgtgggcac gcgtgccgaa gccgcgctgg aggcagcacg gcgcggactg
    19381 gtcgcaccct gagccacgcc tggttcgcgg ccccgggtcg tgccagtact gctgccggcg
    19441 atccttgccg gcggtccgcc ctccttgcct ccctggcgaa cgtggccatc ctcgcctgcg
    19501 atccggcggc tgcctacccc gcgatcacgg ggtcatccgc accccgcgca tgcggaacgg
    19561 gatcgacacc agccgcatgc cgcggttcac ctgcaccgcg aaatgcaggt gcggaccggt
    19621 ggtgaagccg gtattgccgg acaggccgat ctgctggccg gcagtgaccc gctggccgac
    19681 ccgtaccagc gcaccatccg ccttcaggtg cgcgtaaatg gccatgctgc cgtcgtcgtg
    19741 gaggatccgc acgaagttgg cgcggccgcc gtagcgctcc tgcttgagcc cggccttgct
    19801 gaaatcggac tcgacctgca tcaccgtacc ggcgcgcgcg gccagcaccg tggtgccgat
    19861 gtcggcggcg aagtccagcg catgccggtt ctgttcgtcg gtgtggctga acctgccgcc
    19921 gaagccctgg tcgatgcggg cgtgcgattg tcgcagcggc agcaggtact cgacgtcctg
    19981 cggtcgcgcg ctggggtcgc ccggcacccc gcgcaggctg agttcgaagt tgccggtgcg
    20041 gccgggatcc tgcgcgcgca ggcgcgcgac cagggcgctg ccgcgggccg gcacggtggc
    20101 acgcgcgggc aacggcggat cgccgcgcac gttgctgctg cgggcgaagc cgagcagtac
    20161 ctcgatcggg ccggccaggg tgttgtcggc atggaccagg tattcgccgt tgcctggctc
    20221 cacccgcagc cttgcgatcg cggatggctc caccggcacc gggatctcgc gcaccttcat
    20281 cggcgtggca ttggccggcg ggcggtcgcc gtaatgcacg cggccgtcct tgtcgaccca
    20341 gcggtagacc ttgtcgcccg cgtgcgccgc cgacagcgcc accgccaggc cggccacgat
    20401 ccaccagtgg ctgcgcggcg tcatgcgcgg cccgcgccgg tggcaaccgc ctcggctgcg
    20461 gggccgggtt gcacggccag cgcgcggatg cgccgccgca tcgggaactc gtagacgtga
    20521 taggtggcgc tggccgcaag gatggtggcg gccaggaaca gggccagccc gccgccgccg
    20581 ctggcaagcc cggcaccccg catgaagagc aggatcggga agtgcagcag gtacagcgag
    20641 tacgagatct tgccgaggtg gtcgccgatg cggttctcca gcagcgggtt gccctcgggc
    20701 accaggaaca ccagcgccgc gaagatcgcc gacatcgcca gcaggatcgc cgggttgaac
    20761 cacatcacgt aggtccagcc ggtcagcctt tcgaggatcg ccgggtacag cagcaacagc
    20821 atcggcagcg ccagcaccca gccgtggtgg cggaggcggg cgggtgcttg ccagcgcttg
    20881 taggcgatgc cgatcagcat gccggcggtg aagtagggca gcccgcgcag cagcggcagg
    20941 tcgaccggga agccgaacac cggctgcttc gggcccagcg gccaatggcc ggatgcgaac
    21001 gcccccagca ccaccacgct gaacaacggg atcgcccacg ccagccgcgg ccgcagccac
    21061 cagagcgccg cgaagaccag gtagaagtgc agctccgccg ggatcgtcca cagcacctgt
    21121 accccgtaca gggtcagcag gtgcgaggcc agcgacttca ggtccgggat gtcgtaggcc
    21181 gcggccatca gcgcgggcca gccggattgc tgggccagcc acgacagcag cacgatcgcc
    21241 acgaacagcg gcaggatccg ggccgcgcgc gcgctggcgt aaccgcgcag cgccggcgcg
    21301 gtgggtgggc ggtccaggta cagcaccgtc atcaggaacg cgctgagcag gaagaacagc
    21361 atcaccccga gctgcggcgc gcgcgtgccc agcaccgcgt cccacaactg cgcccggttg
    21421 ctgtagtggc caaccagcac gaccagtgcg gcgatgccac gcaacgtgtt caagcgacgg
    21481 atctgcattc ccggcttcct gcaacggcgg ggcaagtatt cccgatatcg ccggctcatg
    21541 acgattggtt atatccatcg ctccatccgg ataaagcgat tgacatgtcc ggcagccgtg
    21601 cccagactgc agccatccat cgagaccggc tgagggacag gcccgaagaa gccggggcaa
    21661 cctgatggag cgtgctgcca gtgcagcctg ctcgatcgag gtgccaaatc ctgcggggac
    21721 ctgcgtgtcc accggaagat ggttgcgatg ccgcgactcg cgcggcccgc accgccgacc
    21781 cgtgtcggcc ttccggttcc cccgcgcgac tccgatggaa gccaccccgt cggagccatg
    21841 ccgtgaccct tgccgaacgc accgaacttc gccgcccgca cctcccccag ggcgcaacgt
    21901 cgatgcgtgc cgcgcaggcc gtgggcccgg acgtgggcaa cgtgcgcatc ggcagcatcg
    21961 atgcggtgct cgccacccgc catgccggca cccgcaagtt gcgcctggcc tatgagttgg
    22021 tcggccccgc gggtgcgccg gtggtggtgg tcgccggcgg catttcagcg caccggcacg
    22081 tggccgccaa tgcggtggat gccagcagcg gctgggccga aggcctcacc ggcgcgggcc
    22141 gggcgatcga tccgtcgcgc cagcagttgc tcgccttcga ctacgtcggc gcctgcggcg
    22201 gcatcgacgc accgatcgac accgccgacc aggccgatgc ggtcgcgctg ctgctggacg
    22261 cgctcggcat cgacgcgctg caggcgttcg tcggctattc gtatggcgcg ctggttggcc
    22321 tgcagctggc ggcgcggcac ccgcggcggc tgcacaagct ggtcgcggtc agcggcgcgc
    22381 accgcccgca tccgtatgcc tcggcgtggc gggcgctgca acgcaaggcg gtcgcgctgg
    22441 gccagctgca gtgcgccgac gagcaggggc tgtcgctggc gcgccagttc gcgatgctga
    22501 gctaccgcac gccggaggaa ttcggcgagc gcttcgatgc cgcgccaacc ctggagaacg
    22561 gccacgtccg ggttgccgcc gaggactacc tggaagcggc cggtgcgaag ttcgtcgccc
    22621 gcatcccggc caccgcctgg ctgcggttgt ccgaatcgat cgacctgcac caggtcgacc
    22681 cgaccctgat cgaggtgccg acgctcgtcg tcgcggtcga aggcgaccgc ctggtgccgc
    22741 tgtccgatgc cgtcgggctg gtcgaaggcc tcggcctcaa cggccggctg cgggtgctgc
    22801 gttcgcagta cgggcacgac gccttcctga aggaaaccga ccgcatcgat gcgctgctgg
    22861 ccgccgaact gggcgccagc ggcgtgcgcg acgcggttcc cgccattccc cacgatttca
    22921 tcggagcttc cgtatgagcc aggtccagtc cgtcagcggc gcgcgccgcg ccacccgcag
    22981 cgtgcgcgcc ggcatcgacc gggataccgc gttcggggcg gtcaccccgc cgctggtgct
    23041 gtcgtccaac ttcagcttcg ccggcttcgc cgagaagcgg cagtacgact acacgcgcag
    23101 cggcaacccc acccgcgacc tgctgggcga ggcgctggcc gaactcgaag gcggtgccgg
    23161 cggcgtggtg acgtccaccg ggatgtcggc gatcaccctg gtgctcaatg cgttgctgca
    23221 accgggcgac accctggtgg tcccgcacga ctgctacggc ggcagctggc ggctgttcaa
    23281 cgcattggcc aagaagaacg cgttcaagct ggtcacctgc gacctgaccg atccgcgcgc
    23341 gctggccgaa gcgctggaag cctcgccggc gctggtgtgg atcgagaccc cgtccaaccc
    23401 gctgctgcgg atcaccgacc tgcgcttcgt gatcgaggcc gcgcacaagg ccggtgcgct
    23461 ggcggtggtc gacaacacct tcctgtcgcc ggcgctgcag cggccgatcg cagacttcga
    23521 tgccgacgtg gtggtgcatt cgaccaccaa gtacatcaac ggccacagcg acgtggtcgg
    23581 tggcgcggtg atcgcccgcg agcaggcgca acacgagctg ctggcgtggt gggcgaactg
    23641 cctgggcctg accggcgcgc cgttcgacag cttcctgacc ctgcgcgggc tgcgcacgct
    23701 ggacgcgcgc ctgcgcgtgc accaggaaaa cacgcaggcg ctggtcgcgt tgctggacgg
    23761 gcatccggcg gtgcgcgcgc tgcactatcc ggggctggcc tcgcaccccg ggcatgcgtt
    23821 ggcggcgcgc cagcagcacg gcttcggggc gatgctcagc gtcgagctgg aaggcggcga
    23881 ggccgaggtg cgcgccttca tcgacggcct gcagtacttc accctggccg agtcgctggg
    23941 cggcgtggag agcctggtcg cgcatccggc gacgatgacc catgcggcga tgagcgcgga
    24001 ggcgcgcgcc gcggccggca tctccgatgg cctgctgcgg ttgtcggtgg ggatcgagca
    24061 tgtcgacgac ctggtcgccg acatccaggc cgcgctgcag cgcgcgcagg acgccgccgg
    24121 gtgtccacgc aaggtcgcgc gatgaatgca cgcgtcgccc agttgcgcgg ccatgccgcg
    24181 cagcctgcgc gggtggcact gcttggcacc ggcacggtcg gcagcgcggc atgggcgcgg
    24241 ctggcgtcgt gggcgggaac accgctgggc gaacggctgt cgctggtgca cgtggcgaac
    24301 tcgcgggtcg cgatcagcga ccgcaacggg atcaccgcgg atacccgtga actgctgctg
    24361 cacaatgccc cgcgcgaaag ttcgctggcg gcggtggccg atgcgctggc gggcaccggc
    24421 acccgcatcg tggtcgatgc gaccgcgtcc gaggcagtcg ccgaccagca cccacgctgg
    24481 ctggccagcg gcatccacgt ggtcaccgcc tgcaagctgg ggcagggcac ctcgctggcg
    24541 cgctggcgcg cgatccagtt cgcgcgcgcg cgcggcggca gcgactacgg cgacagcgcc
    24601 accgttggcg ccggcctgcc gctgctgcgc acgctccggg agttgcaggc cggcggcgac
    24661 cgcatccacg cgatcgccgg ggtgctgtcc ggatcgctgg cctggctgtt caaccactac
    24721 gacggcctgc gtccgttctc cggcttcgtg cgcgccgcgc gcgaggccgg ctacaccgaa
    24781 cccgacccgc gcgacgacct gtccggcgag gacgtgcgcc gcaagctcct gatcctgtcg
    24841 cgcgccgccg gccatgcgct ggaagccgac gcggtgcagg tcgactcgct ggtcccgccc
    24901 gtgctggccg cgcttgcggg tgcggatgtc gatgcggcgt tgcagtgcct ggacgcgccg
    24961 ctgcgcgaac gttacgccgc cgcctacaag aacggcgaga aactgcgttt catcgcgcgc
    25021 ctgcaggccg gcgggctggc cacggtcggg ctggaggcat tgccggcggc gcatccgctg
    25081 gcgggtggcg ccggcaacga caaccgtgtc gcgatctggt cggaccgtta ccgcgagcag
    25141 ccgctggtga tccagggccc gggcgcgggc gcggaggtga ccgccgcggc actgctggac
    25201 gatgccctgc ggatcgcgcg cggacgctag ccgaggtccc cggcgaagaa cgcggcgatc
    25261 ggccgcgggc gctggtacac gtagccctgc gcgtagtcga cgccgatctc gcgcaagcgc
    25321 tgcagctgca gttcggtctc ggcctgttcc gccaccgcgc gcttgccgag cacgtgcgcg
    25381 atctcggtga tcgagcgcac caccgcctcc gacaggctgc cgccttcgtc atggatgtca
    25441 tcgaggtcgc gcacgaagct gccgtcgatc ttcaggtagt cgacgtcaag gtcgcgcagg
    25501 tagccgaacg aacagaagcc ggtgccgaag tcgtccagcg cgaaccggca accgaggtcg
    25561 cgcatgcggc cgatgaagtg gcgggcgcgg gtcatgtcgc gcaccacgct ggtctcggtg
    25621 atctccaggc acagctgttc agcgcgcagc gggctgcggc gcaggcgcgc ggcgacgaag
    25681 tcgccgaagt cctcgtcgac cagggtggtg ccgccgatgt tgatgcagca ctgctcgacc
    25741 tgggcggtgg cctccgggtg ctgttccagc cagccgaggg tcgcgtccag cacgaagcgg
    25801 tccagccgcg gcccgagccg gtagcgttcg gcggcggcca ccagctcagc cggcgggcgc
    25861 aggttgccgt cggtgtccat ccagcgcacc agcacctcga aatgcgattg cggcggggcg
    25921 gggttgcgca ggtccacgat cggctggcac cacaggacga agcggcgctg gtccagcgcc
    25981 tcgcgcgcgg ccatggcgct gtgcatcgca cgggtgcgct ggcgcagttc gtcggggttc
    26041 gatgccgccg cgaacgagcg gttgccgccc agttccttgg ccgcgaagca ggcggcgtcg
    26101 gtctgcgaca agaggtcgtc gaaggcgagg tgcggtggct cgctggtggc cagcccgatg
    26161 ctggcggtgg tgcccaggtt ctggcccagc catgccaccc gcatgcgttc gatcgccgac
    26221 agcaggcggc gcgcgcgggt gcttgcggcg cgctcgtcga tcgtcgccag caccgcgaat
    26281 tcgtcgccgc cggtgcgcgc gatcagggtg tcttcgccga attcttcgcg gaacagcgcg
    26341 gccacgccgc ggatcatctc gtcgccggcg acatggctgg cggagtcgtt gaccagcttg
    26401 aagtggtcga ggtccgcgta cagcagggtc agggtgccgg ttcccgcggc cagttgcgcg
    26461 cgcgcctgtt cctcgaaggc gtcgcggttg agcagcccgg tcagggtgtc gcgggtggca
    26521 cgctggtgca gtgccgcggt cgcggccgcg tgttcatgcc tggatgccag cagcagcacc
    26581 gggatgatcg aatacatcac cagcatcgac atcatcagca cgctgtcgtg catggtctgc
    26641 gggcgctcga ggccacccag ccccagcccg gtcatcaggc tgacgcagac gatcacgatg
    26701 gtggtgccaa gcagggtcac cagcggcgtg tagcgcaccg ccgagaagat cagcaggccc
    26761 agcggcaggc tggccagcgc caacgggtac tgggtgccga ggttgccgac gaggaacacc
    26821 atcgccagca ccagcgcgac cgtcagcgac caggccagca actcgagccg ccggctttcc
    26881 ttgcgtgccg ggctgccctg cttgcggtag aacaggtaga gcagcagcgg ccccagggtg
    26941 ctgacgccca gcaggtcgcc cagcgtccac tgcaggaaca gccgcacgat gtcgccggcg
    27001 ggcgccatgc cggcgtgcag catgccgacg ctgccgatcc ccgcgctgac cacgctgagc
    27061 aacaggccgc cgcgcagcag caggaagccg tcgctggtgc gcaggtggat ccgccggttg
    27121 cggtcgtgcc cgcggcgcac gtaccagccc gcgagcaggg tcgccagcgt gttgctggcc
    27181 agcgagtagc ccaggaacac cggcggcacc ggcaccacga acaggtgcag gagcagcatc
    27241 gcgaccggga tcgtcagggt ctgccacagg ccgtagcgca ggaccgcggc caggccgatg
    27301 cccgcggctg gccagaacag ggtgacgtcg gccggggtgc gcaggtaaaa caccgcgatc
    27361 accgccccgg cgaagtacaa gccagtcagc agtgcccccc gtagcacgct cgccaatcgg
    27421 gcttgcatgg cctgagtcta tgtcgaaaac ggcggaaggg cgaccgcccc ggtgattgcc
    27481 gggttcagca ctcgatgacg ttcacggcca gcccgccgcg gctggtttcc ttgtacttgt
    27541 cctgcatgtc gcggccggtg tcgcgcatgg tccggatcac cttgtcgagg ctgaccttgt
    27601 gcttgccgtc gccgcgcagg gccatccggc tggcgttgat cgccttcacc gcgcccatcg
    27661 cgttgcgctc gatgca
//
LOCUS       DAORUK010000007        26551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_1389_length_26551_cov_20.011398, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000007 DAORUK010000000
VERSION     DAORUK010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 26551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26551
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1389_length_26551_cov_20.011398"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /gene="speA"
                     /locus_tag="PK818_01045"
     CDS             complement(<1..1252)
                     /gene="speA"
                     /locus_tag="PK818_01045"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HQY81353.1"
                     /translation="MSDWSLDLARKTYSIPHWSEGYFEVDSAGRIAVRPKGAAGPVAA
                     LPEVVDAAMAQGAKLPMLVRFPDVLGDRLGKLQAAFAQARQDWDYPGGYTAVYPIKVN
                     QHQGVAGTLASHHGEGFGLEAGSKPELMAVLALSRPGGLIVCNGYKDREYIRLALIGR
                     KLGLQTFIVIEKPSELKLVLEEAQALGVQPGLGVRMRLASLGAGKWQNSGGDKAKFGL
                     NPRQLLDLWKRLRDAGMGDCLQLLHFHMGSQISNVRDIAAGMREATRYFVELSKLGAK
                     ISHVDVGGGLGIDYEGTRSRSYNSVNYGIHHYASSIVQPLAEACAREGLAPPRILTEC
                     GRAMTAHHAVLVTNVSEVERAPEGRVPDLHADESHPVRQLRELHDEMRGRPAVEVFHE
                     AAQAHAEGLSLYALGQIDLVQRARI"
     gene            1408..2262
                     /gene="speE"
                     /locus_tag="PK818_01050"
     CDS             1408..2262
                     /gene="speE"
                     /locus_tag="PK818_01050"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HQY81354.1"
                     /translation="MTESTWLYENFDKAGSSIGFRVSRKLDEVQSPFQHIEIYESTDW
                     GNVMLIDGAMMLTTRDNFLYHEMMSHPALFTHADPKRVVIIGGGDCGTLREVLRHPGV
                     QSATQCDIDEQVTRMAEKYFPELCESNHDPRAELLFDDGIAYMANCEPGSVDIVIVDS
                     TDPVGPAEGLFNKAFFASCHRALKDDGILVQQSESPLVLLDLIREMRAEMGKAGFASF
                     QTLPFPQPCYPTGWWSCTMAKKAANAGFGFREADARGKAFATGYYSADLHRGAQALPP
                     FVAAALAG"
     gene            2372..4264
                     /locus_tag="PK818_01055"
     CDS             2372..4264
                     /locus_tag="PK818_01055"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQY81355.1"
                     /translation="MKAGLLAALLAWPLAAFATTAWHAGAVEPTSTRAAAFDATLQKV
                     VGPESLETSTAAYDADLERLRALLPAGDIAREVRFRSVYCGSTKWKDPKQGLAYSDEA
                     LKRARDARDAVSEARSMLCRATYIMLISGSQRGLPEVEKVIALLGDGQDQQLLAEALE
                     LRGDILSLLGEQAKAMLDFQRARAAYRGAGIDKEVEPLMLSIAVAYRRMGDWPQAQRH
                     FTAAAKRMQDKGDWESVATNLIQLGFLHGESGAPDKALAVFREAERVAIDHDDAFSVN
                     AARLGIAESQVALGQPDTAMATLQMARSGFAAAHDNASEDMLLMLTGQALARQGRHQE
                     ALLRYREALPLVQQDGNERYLAMLFKAQAASKEALGQAQAALADYKSYNDLQMKLQGK
                     MRLEQSRMLEYEYEIRRRDFENRQLRAEAEVKQQEVAALEAVRRWQWLAIALGLLLVA
                     LLASMAWRQWRRSRRLRDLTLLDPLTGAANRPGIEREASRALNAAQQSGTPLSLLMLD
                     LDHFKAINDRYGHAAGDKVLRATTAAWQAQLRGRDPLGRVGGEEFVVVCPDTSLEQAL
                     VVANRLREAANLLRFDDIDPALRVSVSIGAAQSRKAEESCDALLDRADAALYRAKQQG
                     RDRVES"
     gene            complement(4284..4622)
                     /locus_tag="PK818_01060"
     CDS             complement(4284..4622)
                     /locus_tag="PK818_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HQY81356.1"
                     /translation="MKMVMAIIKPFKLDDVREALAEAGVAGITATEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKIKLEVAVTDDQVDAVSEAIIKAAGTGKIGDGKIFVWDLERVVRI
                     RTGEMDADAL"
     gene            4836..5111
                     /locus_tag="PK818_01065"
     CDS             4836..5111
                     /locus_tag="PK818_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009595755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HQY81357.1"
                     /translation="MIDLAQLDELARRLSSLVPPGLREGREELQQNFKSVLQAGLGKL
                     DLVTREEFEVQRAVLARTREKLEALERDLARLSGDAPGAPAHNPPQH"
     gene            5123..6637
                     /locus_tag="PK818_01070"
     CDS             5123..6637
                     /locus_tag="PK818_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HQY81358.1"
                     /translation="MGLAIVHSRARVGVHAPEVRVEVHLGGGLPSMSIVGLPEAAVRE
                     AKDRVRAAIHCAQFEFPARRITINLAPADLPKHGGRFDLAIALGILAASGQVSGEALD
                     GWEFLGELALTGEIRAVDGVLAAALASGRAGRRLLVPPANGSEAALAAEVEVRTARTL
                     LEVCAALDARKSLPRAEAVACRAAAQPDLADVRGQAQARRALEIAAAGAHHLLFVGPP
                     GSGKTLLASRLCGILPDPDDGEALEAATIASVSGRGLDPARWRQRPFRAPHHTASAVA
                     LVGGGADPRPGEISLAHHGVLFLDELPEWGRHALEVLREPLESGCVTISRAARQAEFP
                     ARFQLVAAMNPCPCGWAGDPSGRCLCNDDMIRRYRARISGPLMERIDLHVEVPRLPPS
                     ALRHDAASGEPSARVRERVVRARAIQQDRCGRANARIDQAQTDAHCRLAARDSALLER
                     AVDSLQLSARSLHRILRVARTIADLADSADIQTPHLTEAIGYRRLERGRERAAA"
     gene            complement(6703..7950)
                     /locus_tag="PK818_01075"
     CDS             complement(6703..7950)
                     /locus_tag="PK818_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptide maturation dehydrogenase"
                     /protein_id="HQY81359.1"
                     /translation="MEARMRLRRCAAAWLEPREVAAFQLDDLMAGGTGVVSRLAWFAH
                     APHLSAAVEVQASQVPVLGAVGPLDWVERADLDARFGADAVDALLHAALLLPQPDGED
                     ASADARFRAQGWHPASAVAHIATRWEGVDGPKGMAEQDLDSNEGLLRKHGAPPPHAMP
                     SADPQADVLLPRVDRDAFDGLLDTRATCRNYDTEAAVPLATFSQLMARVFGARGIGHP
                     APGFDVYKRTSPSGGSLHPTDCFLIVQRVDGIAPGLYRYRIDTHALEPVAPLVAPPQP
                     GDAGTEPLGDAAPRAWNSDELRAFARIAVAGQDFFADAPVLCVLAPRFRRNFWKYRNH
                     AKAYRVCVLDAGHLSQTLQLCATQAGLGPFVTGAINEVDLERAFGMAACEQSPLAVCG
                     FGKRAATLVTSEFDPNRRLWPRE"
     gene            8011..8295
                     /locus_tag="PK818_01080"
     CDS             8011..8295
                     /locus_tag="PK818_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP-related RiPP peptide"
                     /protein_id="HQY81360.1"
                     /translation="MTQEDKIPPAGLHPRLVKKLLDNLEGNDAFRAQFQASPEQALRS
                     IGYTDPWACMQVTSGKLASPEQIKAQRNKLEDRMVGIQNQSCDLDAQEGF"
     gene            complement(8348..10855)
                     /locus_tag="PK818_01085"
     CDS             complement(8348..10855)
                     /locus_tag="PK818_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptide modification system cyclase"
                     /protein_id="HQY81361.1"
                     /translation="MGVNGDTPQVRTLLLTDLVDSTQLVEMLGDGPAAELFRAHDRIV
                     LALQQRWRGRLIDRSDGLLLLFERPIDGLGFALDYVRGLRDLGRERQLKQPLQARAGL
                     HVGEVLVWENSADAVRVGAKSLEVEGLAKPLAGRLMALARPGQILLSATAEPLAHRAA
                     RELGERGELLVWKSYGRWRFKGVPEAQEVFEVGEPGLAPLRMPAHTPKAWRDLPLWRR
                     PVALAAETLLLAGLVLGGWFLTRPQPAIAFGERDWVVVGDLRNLTGDARLDESLEQAF
                     RISLEQSRYVNVISDLKVRETLGRMQAKAGTRVDRAIGSEIAQRDGARALLLPTVAEV
                     GGRLRVSAEVVDPRTQTTVYAVSSDGKGIGSALASIDEVTDQLREKLGEAMQNVQKTS
                     VPLPNVATPSLEALKAFAVARHVANTSRDREQALGLYRRALQLDPQFALAHADMGRLH
                     AQLGEVAPSREAWRRALALPNRLSPQEKLGVELMLLQNGAPGPYLRKAREYVALYPDD
                     SRVIARLATNVWHHRNDFRAAEALGRQALKPQFERTPGARYSVGIYLLGQDKYDAALL
                     EFARARAEGFTGAGELYARAHDARDQHAQADKVFLASTGGRDGWHGEAAVVTWIDRGE
                     PLRALEAAREWAAKSDADADLLESLRARAAIASVAVLSGQGDVAAALRELLAAVDANG
                     EDADGMYPPTSTEIRLYVGLLAAHRGDAGAVAEMLRRTRDSRIVRDYPTVAQLQQVLL
                     AEQDRLAGKPQAAIDRLAPLAKQETSLVAVHWALERAARSAGDAALANEQHQWLRTHR
                     GRVYAESTTTDLLRFFNTAVSREARRQGQQADAAGTR"
     gene            complement(10863..11867)
                     /locus_tag="PK818_01090"
     CDS             complement(10863..11867)
                     /locus_tag="PK818_01090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQY81362.1"
                     /translation="MAAIERDAAGAFDQGDPQSPALTAAEYALFAQVARSRRVDAGER
                     LFARGDLGTLMYVIASGAVDLDFGDDLVCKRLGVREFFGELGLLIGDHARSAAAVMSE
                     AGVLLELGRDEFDALAARDPHLLAQFLRRAIMRVVFNEQALIGRLRRRNQELQSTLDT
                     LRATAHRLNQTEALTRTDELTGLSNRRGFGMHIEQRRRNSTLAGLTLVLIDCDRFKHV
                     NDAHGHLAGDRVLQSVASLLRAVAGPDDIACRLGGDEFCLLVQGHRREEMERLADYIL
                     GSARSLSRMHHNPPQMTTLSLGGCTVGVEGDWEHWYTQADTALYRAKRLGGDRVEWQD
                     "
     gene            complement(12037..13335)
                     /gene="aceA"
                     /locus_tag="PK818_01095"
     CDS             complement(12037..13335)
                     /gene="aceA"
                     /locus_tag="PK818_01095"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HQY81363.1"
                     /translation="MKHTMPTAEQLKLDWANNPRWAGVTRTYSAEDVVRLRGTVAIEH
                     SLARLGAEKLWKSLHKEDFVNALGALTGNQAMQQVKAGLKAIYLSGWQVAADANVAGE
                     MYPDQSLYPVNSVPQVVKRINNTLLRADQLHHAEGDDTIDFLQPIVADAEAGFGGVLN
                     AYELMKAMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTREAVEKLTAARLAADVMGV
                     PTLIVARTDAEAADLVTSDVDDNDKPFCTGERTVEGFYKTHKGLDQAISRGLAYAPYA
                     DLVWCETGKPDLEFARKFAEAIHAKFPGKLLAYNCSPSFNWKKNLDDATIARFQKELG
                     AMGYKFQFITLAGFHALNYGMFDLAHGYARRQMSAFVDLQEKEFAAAERGFTAVKHQR
                     EVGTGYFDTITQTIQGGQSSTVALKGSTEEEQFHGERAAA"
     gene            complement(13382..14995)
                     /gene="aceB"
                     /locus_tag="PK818_01100"
     CDS             complement(13382..14995)
                     /gene="aceB"
                     /locus_tag="PK818_01100"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HQY81364.1"
                     /translation="MQGGVEVTRAVAGQESLLTPAALAFLAGLHRRFDGERQARLSAR
                     AARQAAFDAGALPDFRADTAAIRAGAWTVAPIPAALQDRRVEITGPVDPKMVINALNS
                     GANCYMADFEDSTSPTWANVLEGQHALRKAVAGSLDWMAPDGSKHYTLKPAAERAVLI
                     VRPRGWHLDEKHVLVDGARMSASPISASIFDLGLFCFHNAVALAAQDRGPYFYLPKLQ
                     SMEEAALWDAVLGDIEDTLGLAHGQLKATVLIETLPAAFEMDEILHALKDRIVGLNCG
                     RWDYIFSYIKTFRRHRDRVLPERAQVGMTQPFLKAYSELLIRTCHRRGAHAMGGMAAQ
                     IPISGDAGANDAALARVRADKLREVTAGHDGTWVAHPALIPLAREIFDARMPEAHQRH
                     VLREDVQVSRDDLIRPSLGTITRAGFENNVEVCVRYLAAWLDGNGCVPIHWLMEDAAT
                     AEIARSQLWQWLHFGDGGGEPLHLDDGTPVDFALLERALIGLPSKFSDRLRMPGSSRI
                     NEAIGMLDRLTHADVLVDFLTLPAYERLD"
     gene            15144..16112
                     /locus_tag="PK818_01105"
     CDS             15144..16112
                     /locus_tag="PK818_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQY81365.1"
                     /translation="MAETGPSALRFAYKGSRLKPLRAFCQVARLGSVSRAAEALYLSQ
                     PAVTLQLQALERELGVRLLERSGRRLTPTREGELLYEMARPLVEGLDGLAASFREQVR
                     GLDAGELNVAAGSSTILYLLPGIVDAFRQRHADVRLSLHNVTGASGLDLLRNDAVDLA
                     VGSMLDVPADLSYAPVYRFEPMLIMPLDHPLADKRDLSLEDLSPYGLILPPKRLTTYR
                     LVDLVFQQNRVPYTVALEVGGWEVIKQYVAMGLGISIVTAICLTEADRQRLAARSLAR
                     WFPSRSYGIVMRKGKFLSAQARAFVELIKPELLASREYDQSGHSER"
     gene            complement(16169..18109)
                     /locus_tag="PK818_01110"
     CDS             complement(16169..18109)
                     /locus_tag="PK818_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HQY81366.1"
                     /translation="MKRCKGWAVVLAALLAACPAMPLLAADTGRYLLGDTTAATPGKT
                     APGLLLMGGGDRNFEALRWFMKKAGNGHIVVLRASQAGEIGEEFFNEVGGIRSVETFV
                     FNDREASTDPRILAALKRADGIFLAGGDQSRYVRFWRGTPVAEALDAHVRAGKPLGGT
                     SAGLAMLGEYLYGAMDGGSQTSPRALADPLGAENTIETGFLRIALLKGVVTDTHFSER
                     NRLGRLIAFVAKGEAMAGRPLIGLGVDEDAAVAVEGDGSARVYATSPMAGATVVRGGL
                     AKQVEDEAMQLERVETIGAGPNSVLHLPDGRVERPVFQRRYAVRDGVLAALDAPLLVI
                     HGGAGVEPGDLTREEEQAARAALEAALRAGHARLKAGGSSVDAVAATITVLEDAPQFN
                     AGRGAVFTHDGRNELDTSIMDGATGKAGAAAGLHRVKNPITLARAIMDRSRHVMMVGE
                     GAETFAQEQGVALVDPSYFRTEKRWQQLQKALQEESTAKEVGARLVLAGKAYFGTVGA
                     LALDAQGRLAAGTSTGGMTNKRYGRVGDAPILGAGTWADARCAVSGTGWGEFYIRAAA
                     AHEICARVRLAGQGIVRASEGVINGDIPKAGGDGGAIALDAQGVAAFPFNTGGMYRGW
                     IGADGVPHVAIYRDDALPLPAY"
     gene            18209..18784
                     /locus_tag="PK818_01115"
     CDS             18209..18784
                     /locus_tag="PK818_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQY81367.1"
                     /translation="MPTALDRWFIDAILVHEEALLRYLQRNWPHRDELHDLRQEVYAR
                     VYQAAGKGLPQQPKAFLFASARHLLADRARRARVVSIEPMGDFEPSHVLVDEVSPERW
                     CGGRQALKRLAEALDRLPDRCREVVWLRRVDELSQKDVALRLGISEKTVEKHIAKGMH
                     LLAASLFSGDLPSRDGAPAIPEHDDERRRAD"
     gene            18756..19850
                     /locus_tag="PK818_01120"
     CDS             18756..19850
                     /locus_tag="PK818_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HQY81368.1"
                     /translation="MTMNGDARTDAERVAADWLARQDGGALAPADAAALEAWLGADTR
                     HRVAFLRLLAAWDESRRLQALGAGWPHAGPPPRGHWATPAHGHGIASLGDDAPPLPAP
                     PDLRELRLAPRTHTARRPAARAVAALCLAACGLLATWGWQSYHRVESALYGTALGGIE
                     TVSLSDGSQAILASDSLIEVQLSRRQRRIELARGEAIFDVAKDSRRPFVVDGGDREVV
                     AIGTRFSVRRDARDLRVVVTEGTVRLQSPAGGKRGTRPTTLLPAGSMALVRADGVLVR
                     SIPIADAERLLDWRNGLLVFRDATLADVAAEFNRYNARKLVVADADAGALRIGGSFRW
                     DNAEGFARLLERGFPVRAAYAADSIVLASP"
     gene            19926..23015
                     /locus_tag="PK818_01125"
     CDS             19926..23015
                     /locus_tag="PK818_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY81369.1"
                     /translation="MKPSCPLRVLFLSLACSAALAAQAQGGRVDVPAGDLATALDTYA
                     RQSGTQLVYRADQLKGARSGGLKGQPASPQALNALLKGSGFHAQRDESGAVLIVPQAA
                     PRPTSPAPANRRPAAPQPQSNAAPPVTDIATVQVTGSRIPRAQVEGPAPITMITAEQI
                     QAAGLTSVPDVLRSLSQNSGSVQGQQNTTSAQSTPGAQAVDLRGLGPNHTLVLINGRR
                     IADFPLPLNSRSNFTDIGNIPLGMIDRVEVLTGSASAVYGSDAMAGVINFILKKSTDG
                     TIIDYRYGDTQRGGGESHRLSLTTGFERGEFSGIVGLELLDKRPLWGFDRDIQDSTLD
                     APTSRRRLPRLVAQLYDWDDDVNIGPADDCAAMAGLNDGTTVPSKDRWGEPLCGSERA
                     IAYRTIQNQREGANLYGALEYRFSSELSWFADFQMGRQTVKLLTGTNGNDVASDHMGW
                     EFHDPNSTDNNDKIFFNAGTGHYEIWSRQFAPEEVGGLQNRMNSTTQKTFAITTGFNG
                     TLGEDWTWEAAYNHSEYKAVVKMPRINAEAANRFFLGERLGYDDDGYAIFNPDPARLF
                     TPLTPTEFANIGAMSTWYPVAKNDNLSFTANTPSLFTMPAGDVGFAGAIEYGSQSYKI
                     NPDPLALTADAYYGPRYGDGRGSRDHWSAAGELRVPLLQSLQASVAGRYDMYSYGDKD
                     PGKFTYSMGLEWRPLDTLLVRGSYGTGFRAPDMHYLFADNDYYRTVSTDYYQCRTEEP
                     GYSDADCYDDGSWDVNTFDVYTGNMQLDVETSKSFTAGFVWSPTANFDLAVDYYRIRI
                     ANQVQAQSRERLRADEANCLLGVTDAGVAVDINSPTCVDALARVIRENPNDPYSTITS
                     VHFAPINIANEETSGIDVTANYRLQSASAGDFRFTGNYTWADRHSRQQYPGDPEEDML
                     DLSFSATTLPRTKGNLGVDWDRNAWGAGLFGNYVGRVANYNNDVWTPATWRFNGSARY
                     DISDHLRVSLSVNNLLDKMPPKDATWANYPYYDTSWFDSMGRSYYLQLTWKLGGAPL"
     gene            complement(23082..23705)
                     /locus_tag="PK818_01130"
     CDS             complement(23082..23705)
                     /locus_tag="PK818_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008572229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQY81370.1"
                     /translation="MSDARDNDVVLALPPGAPRMPRNRLMEGLGRGILRLFGWRMAGA
                     FPDLPRLVLIAAPHSSNWDGLLGFAVKFGMGVKLSILGKDSLMRVPLLRALLRWQGVI
                     PVDRSSPHGVVGQAVEAIRSADRMWYAMAPEGTRKRVERWRPGFWHIAHDAGVPVVPA
                     YFDYANKVIGIGPPFELTDDMHADIARIQRWYKPFKGRNHDVVQPAD"
     gene            complement(23702..24118)
                     /gene="arfB"
                     /locus_tag="PK818_01135"
     CDS             complement(23702..24118)
                     /gene="arfB"
                     /locus_tag="PK818_01135"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HQY81371.1"
                     /translation="MLQVGSIAIDDDELIERFVRASGPGGQNVNKVATAVELRFDIAR
                     SPSLPEPVRLRLLARRDRRVTDDGVLVLAAQRFRTQERNRQDARERLAHFIEGGLHAP
                     KPRVATKPTRGARERRLGEKRGRATIKKGRTGRDWD"
     gene            complement(24212..24976)
                     /locus_tag="PK818_01140"
     CDS             complement(24212..24976)
                     /locus_tag="PK818_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQY81372.1"
                     /translation="MESVPVRDNGGSLAKRCRPPMSDSSSLPVLYQDEALAVVDKPAG
                     LMAHDSALARGETDFAADRLREQFGKPIFLVHRLDRATSGCLLLAFDRETASALGKTL
                     MSGEVAKDYWAVCRGWPAEQRFVVDHPLDGGPGKPLKKPAQTAFEVLATCELPVPSAG
                     FETSRYALLQASPLTGRFRQIRRHLKHLSHHLIGDSSHGDGRHNRNFRMLGIHRMLLH
                     ARRLAFVHPHTGARLDVAAPVDAEFARALALFDRPL"
     gene            24975..>26551
                     /locus_tag="PK818_01145"
     CDS             24975..>26551
                     /locus_tag="PK818_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQY81373.1"
                     /translation="MTHHRLHALALACLLAMPASAFAAGVAPPTGISAGACVEGICEY
                     RLRNGLRVLLFPDASKPTVTVNLTYLVGSVHENYGETGMAHLLEHLLFKGTPKHADIP
                     GELKKRGVGFNASTSLDRTNYFSSFPANDATLDYVLGLEADRMLNSFVAKKDLDSEMT
                     VVRNELERNENNPGSVLNERLRSTAYLWHNYGNTTIGARSDVERVPIERLQAFYKTWY
                     QPDNAVLIVAGRFDPATLLPNIKVKFGGLGKPSRALPVAYTKEPAQDGEREVTVRRSG
                     DIRITSLAYHMPAATHPDTPALVVLGNLLGHAPGGRLHKALVESKLAAFAGAGAGAMR
                     DPGTFNFVVAVPKDGDAGKAEAELLKQAEAIASQPVTAQELADARQRIANGYELAFNN
                     VNAVGMALSEYVAAGDWRLWFVLRDATDKVTLDDVNRVAKAYLLPSNRTLARFVPTDN
                     AVRATIAEAPPAASLVEGYTGRAAIEAGESFDPTPANIAARTETYTIGNSLTVSLLPK
                     KTRGGTVVFNADFRFGDL"
BASE COUNT         4112 a         9523 c         8960 g         3956 t
ORIGIN      
        1 cgatgcgcgc gcgctggacc aggtcgatct ggccgagcgc atacaggctc aggccctcgg
       61 catgcgcctg cgcggcctcg tggaagacct ccaccgccgg gcgcccgcgc atttcgtcat
      121 gcagttcgcg cagctgccgc accgggtgcg attcgtccgc gtgcaggtcg ggcacgcgcc
      181 cctccggcgc acgctcgacc tcggacacgt tggtgaccag taccgcgtgg tgcgcggtca
      241 tcgcgcggcc gcactcggtg aggatccgcg gcggcgccag gccctcgcgc gcgcacgcct
      301 cggccagcgg ctgcacgatg ctcgatgcgt agtggtggat gccgtagttg accgagttgt
      361 agctgcgcga acgggtgcct tcatagtcga tgccgaggcc gccgccgacg tccacgtgcg
      421 agatcttcgc gccgagcttg ctgagttcga cgaagtagcg ggtggcctcg cgcatgccgg
      481 cggcgatgtc gcgcacgttg ctgatctggc tgcccatgtg gaaatgcagc agctgcaggc
      541 agtcgcccat gccggcgtcg cgcaggcgct tccacaggtc cagcagctgg cgcggattga
      601 gcccgaactt ggccttgtcg ccgccgctgt tctgccactt gccggcgcca aggctggcca
      661 gccgcatgcg cacgcccagc cccggctgca cccccagcgc ctgcgcctcc tccagcacca
      721 gcttcagttc ggagggcttc tcgatcacga tgaaggtctg caggcccagc ttgcgcccga
      781 tcagggccag ccggatgtac tcgcggtcct tgtagccgtt gcagacgatc agcccgccgg
      841 gccggctcaa tgccagcacc gccatcagct ccggcttgct gccggcttcc aggccaaagc
      901 cttcgccgtg gtgggatgcc agcgtcccgg ccacgccctg gtgctggttg accttgatcg
      961 ggtacaccgc ggtgtagccg cccgggtagt cccagtcctg cctggcctgc gcgaacgcgg
     1021 cctgcagctt gccgaggcga tcgcccagca cgtccgggaa gcgcaccagc atcggcagct
     1081 tggcgccctg cgccatcgcc gcgtccacca cctccggcag ggccgccacc gggccggcgg
     1141 cgcccttcgg ccggactgcg atgcggccgg cggaatcgac ctcgaaatag ccttccgacc
     1201 agtgcgggat cgaataggtc ttgcgggcga ggtcaagcga ccagtcggac attgcggggc
     1261 accggtggaa cgggggggac gcgcattgta gggcgggcct tggcccgttg ccggatccgc
     1321 gccggcacgc ccctgcccgc atcgccgtgg aagcgcggca ggccccgctc tacaatgcgc
     1381 gcccaccccg ctgcccggaa ccgcgccatg accgaatcga cctggctgta cgagaacttc
     1441 gacaaggccg gctcgtcgat cggcttccgc gtgagccgca agctggacga ggtgcagtcg
     1501 ccgttccagc acatcgagat ctacgagtcg accgactggg gcaacgtcat gctgatcgac
     1561 ggcgcgatga tgctgaccac ccgcgacaac ttcctctacc acgagatgat gtcgcacccg
     1621 gcgctgttca cccacgccga cccgaagcgc gtggtgatca tcggcggcgg cgactgcggc
     1681 accctgcgcg aagtgttgcg ccacccgggc gtgcagtccg ccacccagtg cgacatcgac
     1741 gagcaggtca cgcggatggc ggagaagtac ttccccgagc tgtgcgagtc caaccacgac
     1801 ccgcgcgccg agctgctgtt cgacgacggc atcgcctaca tggcgaactg cgagccgggc
     1861 agcgtggaca tcgtgatcgt ggactccacc gacccggtcg gcccggccga aggcctgttc
     1921 aacaaggcct tcttcgcaag ctgccaccgc gcgctgaagg acgacggcat cctggtgcag
     1981 cagtcggaat cgccgctggt gctgctcgac ctgatccgcg aaatgcgcgc ggagatgggc
     2041 aaggccggct tcgccagctt ccagaccctg ccgttcccgc agccctgcta cccgaccggc
     2101 tggtggagct gcacgatggc gaagaaggcc gcgaacgccg gcttcggctt ccgcgaagcg
     2161 gacgcgcgcg gcaaggcctt cgccaccggc tactacagcg ccgacctgca caggggcgcg
     2221 caggcgctgc cgccgttcgt ggccgcggcg ctggcgggct gaccggcagc acacggaaac
     2281 gcgacacccg gcacggaagg cgtatcgttg caccgatagc gtgtcgggtc gtccgacgca
     2341 cggggagcgc gtggcaacac agtcgcatcc gatgaaggct ggcctgctgg ccgcgctgct
     2401 ggcgtggcca ctggccgcgt tcgccaccac ggcgtggcat gcgggcgccg tcgagccaac
     2461 gtcgacccgt gccgcggcgt tcgatgcgac cctgcagaag gtggtcggtc cggaatccct
     2521 cgagaccagc accgccgcct acgacgccga cctcgaacgc ctgcgcgccc tgctgccggc
     2581 cggcgacatc gcgcgcgaag tgcgcttccg ttcggtgtat tgcggcagca ccaagtggaa
     2641 ggacccgaag cagggcctgg cgtattccga cgaggcgctg aagcgcgccc gcgacgcccg
     2701 cgacgccgta tccgaggcac gctcgatgct gtgccgcgca acctacatca tgctgatcag
     2761 cggctcgcag cgcggcctgc cggaagtgga gaaggtcatc gccctgcttg gcgacggcca
     2821 ggaccagcag ttgctggcgg aagcgctgga actgcgcggg gacatcctct ccctgctcgg
     2881 cgagcaggcc aaggcgatgc tggatttcca gcgcgcgcgc gcggcctacc gcggcgccgg
     2941 catcgacaag gaagtggaac cgctgatgct gagcatcgcg gtggcgtacc ggcgcatggg
     3001 cgactggccg caggcgcagc gccacttcac cgccgcggcc aagcgcatgc aggacaaggg
     3061 cgactgggaa agcgtcgcca ccaacctgat ccagctcggc ttcctgcatg gcgaatccgg
     3121 ggccccggac aaggcgctgg ccgtcttccg cgaagccgag cgcgtggcca tcgaccacga
     3181 cgatgccttc agcgtgaatg cggcccggct cgggatcgcg gaaagccagg tcgcgctcgg
     3241 ccaaccggac accgcgatgg ccaccctgca gatggcgcgc agcggattcg cggcggcgca
     3301 cgacaatgcc agcgaggaca tgctgctgat gctgaccggg caggcgctgg cccgccaggg
     3361 gcgccaccag gaggccctgc tgcgctaccg ggaagcgctg ccgctggtcc agcaggacgg
     3421 caacgagcgc tacctggcga tgctgttcaa ggcgcaggcg gccagcaagg aggccctcgg
     3481 ccaggcgcag gcggcgcttg cggactacaa gagctacaac gacctgcaga tgaagctgca
     3541 agggaagatg cggcttgagc agagccgcat gctcgagtac gaatacgaga tccgccgccg
     3601 cgatttcgag aaccggcaat tgcgcgccga ggcggaggtg aagcaacagg aagtcgccgc
     3661 gctggaggcg gtgcggcgct ggcaatggct ggcgatcgcg ctgggcctgc tgctggtcgc
     3721 cctgctcgca tcgatggcat ggcggcaatg gcggcgttcg cgcaggctgc gcgacctgac
     3781 cctgctcgac ccgctgaccg gggccgccaa ccgccccggc atcgagcgcg aggcgtcgcg
     3841 cgcgctcaac gccgcccagc aaagcggcac gccgctgtcg ctgctgatgc tcgacctcga
     3901 ccacttcaag gcgatcaacg accgctacgg ccatgccgcc ggcgacaagg tgctgcgcgc
     3961 caccaccgcg gcctggcagg cgcagctgcg cgggcgcgac ccgctcggcc gggttggcgg
     4021 cgaggagttc gtggtggtgt gcccggacac cagcctggag caggctctgg tggtcgccaa
     4081 ccgcctgcgc gaagccgcca acctgctgcg tttcgacgac atcgatccgg cgctgcgggt
     4141 cagcgtcagc atcggcgccg cgcagtcacg caaggccgag gaaagctgcg atgccctgct
     4201 cgaccgcgcc gacgccgcgc tgtaccgggc caagcagcag ggacgggacc gggtcgaaag
     4261 ctgaggccgg gtcgcaaccg cgcctacagc gcgtcggcgt ccatctcgcc ggtgcggatc
     4321 cgcaccaccc gctccaggtc ccagacgaag atcttgccgt cgccgatctt gccggtgccg
     4381 gccgccttga tgatcgcttc gctcaccgca tccacctggt cgtcggtgac cgcgacttcc
     4441 agcttgatct tgggcaggaa gtcgaccacg tactcggcgc cgcgatacag ctcggtgtgg
     4501 cccttctggc ggccgaagcc cttgacctcg gtggcggtga tgccggccac gccggcctcg
     4561 gccaatgcct cgcgcacgtc atcgagcttg aacggcttga tgatcgccat gaccatcttc
     4621 atcgggcggc ctccacgggc atctttttcg cagccggatg gctgtgcatg gcaccagcat
     4681 aacgcgggtt cggtgcatac tcggcaggca ccgccgcgcc gcgtttgcgg gattccccta
     4741 ccgcgcgacg tgccctgcgc cgacggcgcc agccaccgcc accgcggatg ctggcctcgt
     4801 ctccgcagga acctcccgcc atgaacaccg cccgcatgat cgacctcgcc caactcgacg
     4861 aactcgcccg ccgcctcagc agcctggtcc cgcccggcct gcgcgagggc cgcgaggaac
     4921 tgcagcagaa cttcaagtcg gtgctgcagg ccggcctcgg caagctggac ctggtcaccc
     4981 gcgaggaatt cgaggtgcag cgcgcggtgc tggcgcgcac ccgcgaaaag ctcgaagcgc
     5041 tggaacgcga cctcgccagg ctgtccggcg acgcgccggg cgctcctgcg cacaacccgc
     5101 cgcagcactg acggccgccg ccatgggcct tgcgatcgtg cacagccgtg cgcgggtggg
     5161 cgtgcacgcg cccgaagtcc gggtcgaggt ccacctcggc ggtggcttgc cgtcgatgag
     5221 catcgtcgga ctgccggaag cggcggtccg cgaagccaag gaccgggtac gcgccgccat
     5281 ccactgcgcg cagttcgagt tcccggcgcg ccggatcacc atcaacctgg ccccggccga
     5341 cctgcccaag cacggcggcc gcttcgacct ggcgatcgcc ctgggcatcc tcgccgccag
     5401 cggccaggtc tcgggcgagg cgctggatgg ctgggaattc ctcggcgaac tggcgttgac
     5461 cggggagatc cgtgcggtcg atggcgtgct cgccgcagcg ctggccagcg gccgtgccgg
     5521 gcgcaggctg ctggtgccgc ctgccaacgg cagcgaggcg gcgctggccg cggaggtgga
     5581 ggtccgcacc gcgcgcaccc tgctggaagt gtgcgccgcg ctggatgcgc gcaagtcgct
     5641 gccgcgcgcc gaagccgttg cctgccgcgc cgcggcgcag cccgatctcg ccgacgtgcg
     5701 cggccaggcg caggcgcggc gcgcgctgga aatcgcggcg gccggtgccc accatttgct
     5761 tttcgtcggc ccgcccggga gcggcaagac cctgctggcg tcgcggctgt gcgggatcct
     5821 gccggacccg gacgacggcg aggctcttga agccgcgacc atcgcctcgg tcagcggccg
     5881 cggcctcgac ccggcgcgct ggcggcaacg cccgttccgc gcgccgcacc acaccgccag
     5941 cgcggtcgcg ctggtcggcg gcggtgccga tccgcgccca ggcgagatct cgctggcgca
     6001 ccacggcgtg ctgttcctcg acgaattgcc ggaatggggc cggcacgcgc tggaagtgct
     6061 gcgcgagccg ctggagtcag gctgcgtcac catttcgcgc gcagcgcgcc aggccgaatt
     6121 ccccgcgcgc ttccagctgg tcgcggcgat gaatccctgc ccctgcggct gggccggcga
     6181 cccatcggga cgttgcctgt gcaatgacga catgatccgc cgctaccgcg cgcgcatctc
     6241 cgggccgctg atggaacgca tcgacctgca tgtcgaagtc ccgcggctgc cacccagcgc
     6301 actgcgccac gatgccgcca gcggcgagcc gagcgcacgc gtgcgcgaac gggtggtgcg
     6361 cgcgcgcgcg atccaacagg accgctgtgg cagggccaac gcgcggatcg accaggcgca
     6421 gaccgatgcg cattgccgtt tggcagcgcg cgacagcgcc ctgctcgagc gcgcggtgga
     6481 cagcctgcag ctgtcggcgc gctcgctgca ccggatcctg cgggtggcgc gcacgatcgc
     6541 cgacctggcg gactcggcgg acatccagac cccgcacctc accgaggcga tcggctatcg
     6601 caggctggag cgcggccgcg agcgcgctgc ggcctgagcc ggacgttggc gtcgcgtcac
     6661 gcgacaggca cgcgctgacg ccttgctggc cgggccacgc gctcactcgc gcggccagag
     6721 ccttcggttc gggtcgaatt ccgaagtcac cagcgtcgcc gcgcgcttgc cgaaaccgca
     6781 gacggccaac ggactctgtt cgcaagcagc catcccgaac gcgcgctcga ggtcgacctc
     6841 gttgatcgcg ccggtcacga acggccccag cccggcctgc gtcgcgcaca gctgcagggt
     6901 ctgcgacagg tgcccggcgt ccagcacgca gacgcggtag gccttcgcat ggttgcggta
     6961 cttccagaaa ttgcgacgga agcgcggcgc cagcacgcac agcaccggcg catcggcgaa
     7021 gaagtcctgc ccggccacgg cgatcctcgc gaatgcgcgc agctcgtccg agttccaggc
     7081 acgcggcgct gcatcgccca acggctccgt gccggcatcg cccggttgcg ggggcgcgac
     7141 gagcggtgcc accggctcca gtgcatgggt atcgatgcgg tagcggtaca ggcccggcgc
     7201 aatcccgtcc acccgctgca cgatcaggaa acaatcggtc ggatgcaatg agccgcccga
     7261 gggactggtg cgcttgtaca cgtcgaagcc cggcgcggga tggccgatgc cgcgcgcacc
     7321 gaacacccgc gccatcagct gcgagaaggt cgccagcggc acggccgctt cggtgtcgta
     7381 gttgcggcag gtcgcgcggg tatcgagcaa cccgtcgaaa gcatcgcggt cgacgcgcgg
     7441 cagcaggacg tccgcttgcg ggtccgcgga gggcatggca tgcggcggcg gcgcaccatg
     7501 cttgcgcagc aggccttcgt tgctgtccag gtcctgttcg gccatcccct tggggccgtc
     7561 cacgccttcc cagcgggtcg cgatgtgcgc gaccgccgac gccggatgcc agccttgcgc
     7621 gcggaagcgg gcatctgcgg aggcgtcttc gccatccggc tgcgggagca acagcgcggc
     7681 gtgcagcagg gcgtcaaccg catcggctcc gaagcgcgca tcgaggtcgg cgcgctcgac
     7741 ccagtcgagc ggacccaccg cgccaagcac gggcacctgg ctggcttgca cttccaccgc
     7801 cgcggaaaga tgcggcgcat gcgcgaacca cgccagccgc gacaccacgc cggtgccccc
     7861 ggccatcagg tcatccaact ggaaggcggc cacctcgcga ggttccagcc acgccgccgc
     7921 gcatcgcctg agtcgcatcc tcgcctccat ccggatatcc gcccgcggat ggtattgtcc
     7981 ggcttccatc cacccagggg aaaatccacg atgacacagg aagacaagat cccgccagcc
     8041 ggattgcatc cgcggctggt caagaaactg ctggacaacc tcgaaggcaa cgacgcattc
     8101 cgcgcccagt tccaggcatc gccggaacag gctttgcgtt cgatcggcta caccgacccc
     8161 tgggcgtgca tgcaggtgac gagcgggaaa ctggcgtcac cggagcagat caaggcgcag
     8221 cgcaacaagc tggaggaccg catggtcggc atccagaacc agagttgcga cctggacgcc
     8281 caggaaggct tctgacggca ctgagcatcc gcaagggccc ggcaggcaac tgccgggcct
     8341 ttccttttca gcgggtaccc gccgcgtccg cctgctgtcc ctgccgccgg gcctcgcgcg
     8401 ataccgcggt gttgaagaag cgaagcaggt cggtcgtcgt gctttcggcg taaacgcggc
     8461 cacggtgcgt gcgcagccac tggtgctgct cattcgccag cgcagcatcg ccggcagacc
     8521 tggcggcgcg ctccagcgcc caatgcaccg ccaccagcga ggtttcctgc ttcgccagcg
     8581 gcgcgagccg gtcgatcgcc gcctgcggct ttcctgcgag ccggtcctgt tccgcaagca
     8641 gcacttgctg caactgcgcc acggtcgggt agtcgcgcac gatgcggctg tcccgggttc
     8701 gccggagcat ctccgccacc gcacccgcat cgccccgatg ggcagccagc aggcccacgt
     8761 aaagacggat ctccgtggat gtcggcgggt acatgccgtc cgcgtcttcg ccattggcat
     8821 cgactgccgc gagaagttcg cgcaaggccg ccgccacgtc gccctggccc gacaacaccg
     8881 ccacgctggc gatcgccgcg cgggcgcgca gcgactccag caaatcagcg tccgcatcgg
     8941 acttcgccgc ccattcccgc gccgcctcca gcgcccgcaa tggctcgcca cgatcgatcc
     9001 aggtcaccac cgcagcctcg ccatgccagc cgtcgcgccc gcccgtgctc gcaaggaaaa
     9061 ccttgtccgc ctgcgcgtgc tggtcgcgcg cgtcgtgggc acgcgcatac agttcgcctg
     9121 cgccggtgaa cccttccgcg cgggcgcgcg cgaactccag caaggccgcg tcgtacttgt
     9181 cctggcccag caggtagatg cccacgctgt agcgggcgcc cggggtccgc tcgaactgcg
     9241 gcttcagggc ttggcgcccc agggcctcgg cggcgcggaa gtcgttgcgg tggtgccaga
     9301 cgttggttgc caggcgggcg atgacacggg aatcatccgg atacagcgcc acgtattccc
     9361 gtgccttgcg caaatacgga ccgggcgcgc cgttctgcag cagcatcagc tcgacgccca
     9421 gcttttcctg cggcgacagg cggttcggca gggcaagcgc ccggcgccac gcctcgcgcg
     9481 agggcgccac ctcgcccagt tgcgcgtgca gcctgcccat gtccgcatgc gccagcgcga
     9541 actgcggatc cagttgcagc gcacgccggt acaggccgag cgcctgttcc cggtcacggc
     9601 tcgtgttggc cacgtgccgc gccaccgcga acgccttcag cgcttccagg ctcggggtgg
     9661 ccacgttcgg cagcggcacc gaggtcttct gcacgttctg catcgcctcg ccgagcttct
     9721 cgcgcagctg gtcggtgacc tcgtcgatcg aggccagcgc cgatccgatg cccttgccgt
     9781 cggaggacac cgcatacacg gtggtctggg tgcgcgggtc gaccacctcc gcgctgaccc
     9841 gcaggcgacc accgacctcg gccacggtcg gcagcagcag cgcgcgcgcg ccatcgcgct
     9901 gcgcgatttc cgaaccgatg gcgcggtcca cccgcgtgcc cgccttcgcc tgcatgcggc
     9961 caagggtctc gcgcaccttc aggtcgctga tcacgttgac gtagcgggac tgctccaggc
    10021 tgatccggaa cgcctgctcc agcgactcgt ccaggcgcgc atcgccggtc aggttgcgca
    10081 ggtcgccgac caccacccag tcgcgttcgc cgaaggcgat cgccggctgc gggcgggtca
    10141 ggaaccagcc gccgaggacg aggcccgcca acaacagggt ttccgcggcc agcgccaccg
    10201 gccgccgcca cagcggcagg tcgcgccagg ccttgggcgt gtgcgcgggc atgcgcagcg
    10261 gtgccagccc gggctcgccg acttcgaaca cttcctgcgc ttccggcacg cccttgaagc
    10321 gccagcggcc ataggacttc cacaccagca attcgccgcg ctcgcccagt tcacgcgcgg
    10381 cgcggtgcgc cagcggttcg gcggtggcgg acagcaggat ctgccccggc cgcgcgaggg
    10441 ccatcaggcg accggccagc ggcttggcca ggccttcgac ctccagcgac ttggcgccga
    10501 cgcggaccgc gtcggcactg ttctcccaca ccagcacctc acccacgtgc aggccggcgc
    10561 gcgcctgcag cggctgcttg agctggcgtt cgcggccgag gtcgcgcagc ccgcgcacgt
    10621 agtcgagggc aaagccaagg ccgtcgatcg gacgttcgaa cagcagcagc aggccgtcgc
    10681 tgcggtcgat caggcggccg cgccagcgtt gttgcagcgc aagcacgatg cggtcgtgcg
    10741 cgcggaacag ttccgccgcc gggccgtcgc cgagcatctc gaccagctgg gtggagtcca
    10801 ccaggtcggt gagcagcagg gtgcggacct gcggggtatc gccattcacg cccatcggcg
    10861 ggtcagtcct gccactcgac gcgatcgccg ccgaggcgct tggcccggta cagcgcggta
    10921 tcggcctggg tgtaccagtg ctcccagtcc ccctccacgc ccacggtgca cccgccgagg
    10981 ctgagcgtgg tcatttgcgg cgggttgtgg tgcatgcgcg acaaggagcg cgcgctgccg
    11041 aggatgtagt cggccaggcg ctccatttct tcgcggcgat ggccctgcac cagcaggcag
    11101 aattcgtcgc cgccgaggcg gcaggcgatg tcgtccgggc cagccaccgc ccgcagcagg
    11161 ctggccacgc tctgcagcac gcgatcaccg gccaggtggc cgtgggcatc gttgacgtgc
    11221 ttgaagcggt cgcagtcgat cagcaccagg gtcagcccgg ccagcgtgct gttgcggcgg
    11281 cgctgctcga tgtgcatgcc gaatccacga cggttggaca gcccggtcag ttcgtcggtg
    11341 cgggtcagcg cctcggtctg gttgagccgg tgcgcggtcg cgcgcagggt atcgagggtg
    11401 gactgcagct cctggttgcg gcggcgcagg cgcccgatca gcgcctgttc gttgaacacc
    11461 acgcgcatga tcgcgcggcg caggaactgc gcgagcaggt gcgggtcgcg cgcggccagc
    11521 gcgtcgaatt cgtcgcgccc cagttccagc agcacgccgg cctcggacat gaccgcggcg
    11581 gcactgcggg cgtggtcgcc gatcagcagg ccgagttcgc cgaagaactc gcgcacaccc
    11641 aggcgcttgc acaccaggtc atcgccgaaa tcgaggtcga ccgcgccgct cgcgatcacg
    11701 tacatgaggg tgccgaggtc gccacgggcg aacaggcgtt cgccggcatc gacgcggcgg
    11761 ctgcgcgcca cctgcgcgaa cagcgcgtat tcggcggcgg tcaacgcagg cgactgcgga
    11821 tcgccctggt cgaaggcgcc ggctgcgtcc cgttcgattg cggccacgcc ttctcccacc
    11881 ccacgccccc cgacacgaat gaaggccgca ggatagcacc ggcccccatc gggattggtc
    11941 acgcagatcg gtggtggaat cggccgcgcc cggcgcggca cggcgcccag tcggtgcatc
    12001 cacccgccgg catcgcgccg gcgggtggtg ttcgcgtcac gcggcggcgc gttcgccgtg
    12061 gaactgctct tcctcggtgc tgcccttcaa cgccaccgtc gagctctgcc cgccctggat
    12121 ggtctgggtg atggtgtcga agtagccggt gccgacttcg cgctggtgct tgaccgcggt
    12181 gaagccgcgc tcggccgccg cgaattcctt ttcctgcagg tcgacgaacg cgctcatctg
    12241 ccggcgcgcg tagccatgcg cgaggtcgaa catgccgtag ttcagcgcgt ggaagccggc
    12301 cagggtgatg aactggaact tgtagcccat cgcgccgagt tccttctgga acctggcgat
    12361 ggtggcgtcg tccaggttct tcttccagtt gaagctgggc gagcagttgt aggccagcag
    12421 cttgcccggg aacttcgcgt ggatcgcctc ggcgaacttg cgcgcgaact ccaggtccgg
    12481 cttgccggtc tcgcaccaca ccaggtcggc gtacggcgca taggccaggc cgcggctgat
    12541 cgcctggtcc aggcccttgt gggtcttgta gaagccttcc acggtgcgct cgccggtgca
    12601 gaacggcttg tcgttgtcgt cgacgtcgga ggtgacgagg tccgcggctt cggcatcggt
    12661 gcgcgccacg atcagggtcg gcacgcccat cacgtcggcg gccaggcgcg cggcggtgag
    12721 cttctcgacc gcctcgcggg tcggcaccag caccttgccg cccatgtgcc cgcacttctt
    12781 caccgaggcc agctggtcct cgaagtgcac gccggcggcg ccggcctcga tcatcgcctt
    12841 catcagctcg taggcgttga gcacgccgcc gaagccggcc tcggcgtcgg ccacgatcgg
    12901 ctgcaggaaa tcgatggtgt cgtcgccttc ggcgtggtgc agctggtcgg cgcgcagcag
    12961 ggtgttgttg atgcgcttga ccacctgcgg caccgagttc accgggtaca gcgactggtc
    13021 ggggtacatc tcgccggcca cgttggcgtc cgccgcgacc tgccagccgc tgaggtagat
    13081 cgccttcagc ccggccttga cctgctgcat ggcctggttg ccggtgagcg cgccgagcgc
    13141 attcacgaaa tcctccttgt gcagggactt ccacagcttc tcggcgccca ggcgggccag
    13201 cgaatgctcg atcgcgacgg tgccgcgcag gcgcaccacg tcctcggcgg aataggtgcg
    13261 ggtgacgccg gcccaacgcg gattgttggc ccagtccagc ttcagttgtt cggcggtcgg
    13321 catggtgtgc ttcatggggc tctctcgtgg ggttggcggt gcggtgaagg ggtgtgatgg
    13381 ttcagtcgag gcgttcgtag gcgggaagcg tgaggaaatc gaccagcacg tcggcatggg
    13441 tgagtcggtc gagcatcccg atcgcctcgt tgatgcggct gctgccgggc atgcgcaggc
    13501 ggtcgctgaa cttgctgggc aggccgatca gggcgcgctc gagcagggcg aaatcgaccg
    13561 gggtaccgtc gtccaggtgc agcggctcgc cgccgccgtc gccgaagtgc agccactgcc
    13621 agagctggct gcgggcgatc tcggcggtgg cggcgtcttc catcagccag tggatcggca
    13681 cgcagccgtt gccatccagc cacgcagcga ggtagcgcac gcagacctcg acgttgttct
    13741 cgaagccggc gcgggtgatc gtgcccagcg acggccggat caggtcgtcg cgcgacacct
    13801 gcacgtcctc gcgcagcacg tggcgctggt gcgcttccgg catgcgcgca tcgaagatct
    13861 cgcgggccag cgggatcagc gccgggtgcg ccacccaggt gccgtcatgg ccggcggtca
    13921 cttcgcgcag cttgtcggcg cgcacgcgcg ccagtgccgc gtcgttcgcc ccggcatcgc
    13981 cgctgatcgg gatctgcgcg gccatcccgc ccatcgcatg cgcgccgcgg cggtggcagg
    14041 tgcggatcag caattccgag tacgccttca ggaacggctg ggtcatgccg acctgcgcgc
    14101 gctccggcag cacccggtcg cggtgccggc ggaaggtctt gatgtacgaa aagatgtagt
    14161 cccagcgccc gcagttgagg ccgacgatgc ggtccttcag cgcgtgcagg atctcgtcca
    14221 tctcgaacgc ggcgggcagg gtctcgatca gcacggtcgc cttgagctgg ccgtgggcca
    14281 ggcccagcgt gtcctcgatg tcgcccagca ccgcatccca cagcgccgcc tcttccatcg
    14341 actgcagctt gggcaggtag aaatacgggc cgcgatcctg cgccgccagc gcgaccgcgt
    14401 tgtggaagca gaacaagccg aggtcaaaaa tgcttgctga aatcggcgag gcggacatgc
    14461 gtgcgccgtc gaccagcacg tgcttctcgt ccaggtgcca gccgcgcggg cgcacgatca
    14521 gcaccgcgcg ctcggccgcc ggcttcagcg tgtagtgctt gctgccatcc ggggccatcc
    14581 agtccagcga gccggccacc gccttgcgca gcgcgtgctg gccttccagc acgttcgccc
    14641 aggtcgggct ggtgctgtcc tcgaagtcgg ccatgtagca gttcgcgccg gagttcagcg
    14701 cgttgatgac catcttcggg tcgaccgggc cggtgatctc cacgcggcgg tcctgcagcg
    14761 cggccgggat cggcgccacg gtccaggcgc cggcgcggat cgcggcggtg tcggcacgga
    14821 agtccggcag ggcgccggca tcgaatgcgg cctggcgcgc ggcgcgggcg gacaggcggg
    14881 cctggcgctc gccgtcgaag cggcgatgca ggcctgccag gaacgccagc gcggccgggg
    14941 tgagcagcga ttcctggccg gccacggcgc gggtgacttc gacgccaccc tgcatgcccg
    15001 gcttcggacc ctcttgcagc actgccgaca tcgccaactc caggacaacg gtggggatac
    15061 gaaccatccg cctgcgtctg gtatttgaca aagcagtttt atcaatgcgt actatttgaa
    15121 ttccaaatag tgtaatcaga tcaatggccg agaccggtcc cagcgccctg cgattcgcct
    15181 acaagggctc ccgcctgaag cccttgcggg cgttttgcca ggtggcgcgg ctgggctcgg
    15241 tgtcgcgcgc ggcggaagcg ctgtacctca gccagcccgc ggtgaccctg cagttgcagg
    15301 cgctggaacg cgaactgggg gtacgcctgc tggagcgcag cggccgccgc ctgaccccca
    15361 cccgcgaggg cgagctgctg tacgagatgg cgcgccccct ggtggagggg ctggacgggc
    15421 tggccgccag cttccgcgaa caggtgcgcg ggctggatgc gggcgagctg aacgtggccg
    15481 ccggcagctc gaccatcctg tacctgctgc ccgggatcgt cgatgccttc cgccagcgcc
    15541 atgcggacgt gcgcctgagc ctgcacaacg tcaccggcgc cagcggcctg gacctgctgc
    15601 gcaacgacgc ggtggacctg gcggtgggct cgatgctgga cgtgccggcc gacctcagct
    15661 acgcgccggt gtaccgcttc gagccgatgc tgatcatgcc gctggaccat ccgctggcgg
    15721 acaagcgcga cctctcgctg gaggacctgt cgccgtacgg cctgatcctg ccgcccaagc
    15781 gcctgaccac ctaccggctg gtcgacctgg tgttccagca gaaccgggtg ccgtacacgg
    15841 tggcgctgga agtcggcggc tgggaggtca tcaagcagta cgtggcgatg ggcctgggga
    15901 tcagcatcgt caccgcgatc tgcctgaccg aagccgaccg ccagcgcctg gccgcgcgct
    15961 cgctggcgcg ctggttcccc tcgcgcagct acgggatcgt gatgcgcaag ggcaagttcc
    16021 tgtcggcgca ggcgcgcgcc ttcgtcgaac tgatcaagcc ggagctgctg gccagccgcg
    16081 agtacgacca gagcgggcat tcggagcgat agacgagcga aggggcggag gacatccatc
    16141 ccctccgccc ctcctcccga gtacctggtc agtaggccgg cagcggcagt gcgtcgtccc
    16201 tgtagatcgc cacgtgcggc acgccatccg cgccgatcca gccgcggtac atgccgccgg
    16261 tattgaacgg gaatgcggcc acgccctgcg catccagcgc gatcgcgccg ccgtcgccgc
    16321 cggccttggg gatgtcgccg ttgatcacgc cctcgctggc gcggacgatg ccttgcccgg
    16381 ccaggcgcac gcgcgcgcag atctcgtgcg cggcggcggc acggatgtag aactcgcccc
    16441 agccggtgcc ggagaccgcg catcgtgcat cggcccaggt gccggcgccg aggatcggcg
    16501 catcgccgac gcggccgtag cgcttgttgg tcatgccgcc ggtcgaggtc cccgcggcca
    16561 gccgcccctg cgcatccagc gccaaggcac cgacggtgcc gaagtaggcc ttgcccgcaa
    16621 gcaccagccg ggcgccgacc tccttcgcgg tgctctcttc ctgcaacgcc ttctgcaact
    16681 gctgccagcg cttctcggtg cggaaatacg agggatcgac cagcgcgacg ccctgctcct
    16741 gcgcgaacgt ttccgcacct tcgccaacca tcatcacgtg ccgcgatcga tccatgatcg
    16801 cgcgggccag ggtaatcggg ttcttcaccc gatgcaggcc cgcggccgcg ccagccttgc
    16861 cggtggcgcc atccatgatc gaggtgtcca gttcattgcg gccgtcgtgg gtgaacaccg
    16921 cgccgcggcc ggcgttgaac tgcggcgcat cctccagcac ggtgatcgtc gccgccactg
    16981 catccacgga cgatccgccc gccttgagcc gcgcatgacc tgcacgcagc gccgcttcca
    17041 gcgccgcccg cgccgcctgt tcttcttccc tggtcaggtc gcccggctcc acgccggcac
    17101 cgccatggat caccagcaac ggcgcatcca gcgcggccag caccccatcg cgcaccgcgt
    17161 agcgacgctg gaacaccggt cgctcgaccc ggccatcggg caggtgcaac acggagttcg
    17221 gccccgcacc gatggtctcg acgcgttcga gttgcatcgc ttcgtcttcg acctgtttgg
    17281 cgaggccgcc gcgcaccacc gtcgcgcccg ccatcggcga agtcgcgtag acgcgcgcac
    17341 tgccatcgcc ttccaccgcg accgcggcgt cttcgtccac gcccaggccg atcaatggcc
    17401 tgcccgccat cgcttcgccc ttggcgacga aggcgatcag gcggccgagc cggttgcgct
    17461 cgctgaaatg ggtgtcggtg accacgccct tcaacagcgc gatgcgcagg aagccggttt
    17521 cgatggtgtt ctcggcgccc agcggatcgg ccagcgcacg cgggctggtc tggctgccgc
    17581 cgtccatcgc gccgtacagg tactcgccga gcatggccag gcctgcgctg gtgccgccaa
    17641 gcggcttgcc tgcgcgcaca tgcgcatcca gcgcctcggc caccggcgtg ccgcgccaga
    17701 agcgcacata gcgggactgg tcgccgccgg ccaggaagat gccgtccgcc cgcttcagcg
    17761 cggccaggat cctcgggtcg gtggatgcct cgcgatcgtt gaacacgaag gtctcgacgg
    17821 agcggatgcc gccgacctcg ttgaagaatt cctccccgat ctcacccgcc tgggaggcgc
    17881 gaagcaccac gatatggcca ttgccggcct tcttcatgaa ccagcgcagc gcctcgaagt
    17941 tgcggtcgcc gccccccatc agcaacaagc ccggcgcggt cttgccgggc gtcgccgcag
    18001 tggtgtcgcc aagcaggtaa cgaccggtgt ccgccgccag cagcggcatc gccggacatg
    18061 ccgccagcaa cgccgcaagc accaccgccc aacccttgca tcgcttcatc gtgatccgcg
    18121 tccttcatcc ggcagtcctg tcctagacga accggggcgc gatttccccc gtgctgcgac
    18181 gcgccgcggc atccgctatc ttcggccgat gccgactgcg ctggacaggt ggttcatcga
    18241 cgcgatcctc gtccacgagg aggccctgct tcgctacctg cagcgcaact ggccgcaccg
    18301 cgacgagttg catgacctgc gccaggaggt ttacgcacgg gtctaccagg ccgcaggcaa
    18361 ggggctgccg cagcaaccca aggcgttcct gttcgccagc gcgcgccacc tgctggccga
    18421 ccgcgcacgc cgtgcaaggg tggtgtcgat cgagcccatg ggtgatttcg agccttcgca
    18481 cgtcttggtg gatgaggtgt cgccggaacg ctggtgcggg ggcaggcaag cgctcaagcg
    18541 actggccgag gccctggacc ggttgcccga ccgctgccgg gaagtggtgt ggctgcgacg
    18601 cgtggacgag ctgtcgcaga aggacgtggc attgcgcctg ggcatcagcg agaagaccgt
    18661 ggaaaagcac atcgccaagg gcatgcacct gctcgccgcc tccctgttca gcggggactt
    18721 gccgtcgcgc gatggcgccc cggcaatccc ggaacatgac gatgaacggc gacgcgcgga
    18781 ctgacgcgga gcgcgtcgcg gccgattggc tggcgcgcca ggacggcggc gcactggcgc
    18841 ccgccgatgc cgccgcattg gaggcgtggc tgggtgcgga cacccggcac cgcgtcgcat
    18901 tcctgcgcct gctggcggca tgggacgaga gccggcgact gcaggcgctg ggcgcgggtt
    18961 ggccgcatgc cggtccgccg ccgcgcggcc attgggccac gcccgcgcat ggccatggca
    19021 tcgcctcgct cggcgacgat gccccgccgc tgcccgcgcc cccggacctg cgcgagctcc
    19081 ggctggcacc acgcacgcac accgcgcgac ggcccgccgc acgcgccgtc gccgcgctct
    19141 gcctggccgc ctgcgggttg cttgcgacct ggggatggca gtcctaccac cgcgtcgagt
    19201 ccgccctcta cggcaccgcc ctgggcggca tcgagacggt gtcgctcagc gacggctcgc
    19261 aggcgatcct ggccagcgac agcctgatcg aggtccagct gtcgcggcgc cagcggcgca
    19321 tcgagctggc ccgcggcgaa gcgatcttcg acgtggccaa ggattcccgc cgcccgttcg
    19381 tcgtcgacgg cggcgaccgc gaagtggtgg ctatcggcac ccgcttctcg gtgcgacgcg
    19441 acgcgcgcga cctgcgcgtg gtggtcaccg aggggacggt tcggctgcag tcaccggcag
    19501 gcggcaagcg gggcacgcgt cccaccacgc tgttgccggc cggcagcatg gccctggtcc
    19561 gcgccgatgg cgtgctggtg cgcagcatcc cgatcgccga cgccgagcgc ctgctggact
    19621 ggcgcaatgg cctgctggtg tttcgcgatg ccacgctggc cgacgtcgcc gcggagttca
    19681 accgctacaa cgcacgcaag ctcgtggtgg ccgacgccga cgccggtgcc ctgcggatcg
    19741 gcggcagctt ccgctgggac aatgcggaag gcttcgcccg gctgctcgaa cgcgggttcc
    19801 cggtgcgcgc cgcatacgcc gcggacagca tcgtgctggc gtcgccctga ccgcgcgggg
    19861 gatttcgcag gctcgttcgt catggagctg taacggcgcc gcgtcgagcg cctgggggaa
    19921 tctgcatgaa accatcgtgt ccgctgcgcg tcctgttcct gtcgctggcc tgttctgccg
    19981 cgctcgccgc gcaggcgcag ggtgggcgcg tcgacgttcc cgccggcgac ctcgccactg
    20041 cgctggatac ctatgcccgg cagtcgggca cccagctggt gtatcgcgcc gaccagttga
    20101 agggcgcgcg cagcggtggc ctgaaaggcc agcccgcatc cccgcaggca ctgaacgcgc
    20161 tgctgaaggg cagcggcttc cacgcacagc gcgatgaatc cggcgcggtc ctgatcgtcc
    20221 cgcaagccgc gcccaggccg acgtcgccgg cgcctgccaa ccgcaggccg gcggccccgc
    20281 agccgcagtc gaacgccgca ccgccggtga cggacatcgc gacagtgcag gtcaccggtt
    20341 cgcgcatccc acgtgcgcag gtagaagggc cggcgccgat caccatgatc accgccgagc
    20401 agatccaggc cgccggcctg acctcggtcc cggatgtgtt gcgctcgctc agccagaaca
    20461 gcggcagcgt gcagggccag cagaacacca ccagcgcgca gtccacgccc ggcgcacagg
    20521 cggtcgacct gcgcggcctt ggccccaacc acacgctggt gctgatcaac ggccgccgca
    20581 tcgccgactt cccgctgccg ctcaacagcc gcagcaactt caccgacatc ggcaacatcc
    20641 cgctgggcat gatcgaccgt gtcgaagtgc tgaccggcag cgcctcggcc gtgtacggct
    20701 cggacgcgat ggccggcgtg atcaacttca tcctcaagaa atccaccgac ggcaccatca
    20761 tcgattaccg ctatggcgac acccagcgcg gtggcggcga atcgcaccgg ttgtcgctga
    20821 ccaccggctt cgagcgcggc gagttcagcg gcatcgtcgg cctggaattg ctcgacaagc
    20881 gtccgctgtg gggctttgac cgcgatatcc aggactcgac cctcgatgcg cccacgtcgc
    20941 gtcgtcgcct gccgcgtctc gtcgcccagc tctacgactg ggacgacgac gtcaacatcg
    21001 gtccggccga cgactgcgcg gcgatggccg gtctcaacga cggcaccacc gtgccgtcca
    21061 aagaccgctg gggcgaaccg ctgtgcggca gcgagcgcgc catcgcgtat cgcacgatcc
    21121 agaaccagcg cgagggcgcg aacctctatg gcgcgctcga gtaccgcttt tcctccgagc
    21181 tgtcgtggtt cgcggacttc cagatgggcc ggcagaccgt caagttgctg accggcacca
    21241 acggcaatga cgtcgccagc gaccacatgg ggtgggaatt ccacgacccg aactcgaccg
    21301 acaacaacga caagatcttc ttcaacgccg gcaccggcca ctacgagatc tggtcgcgcc
    21361 agttcgcgcc ggaggaagtc ggcggcctgc agaaccgcat gaacagcacc acccagaaga
    21421 ccttcgcgat caccaccggc ttcaacggca cgctgggcga ggactggacc tgggaagccg
    21481 cctacaacca ttccgagtac aaggccgtgg tgaagatgcc gcgcatcaat gcggaggcgg
    21541 ccaaccggtt cttcctgggc gaacggctgg gctatgacga tgacggctat gccatcttca
    21601 acccggaccc ggcgcgcctg ttcaccccgc tgacgccgac cgaattcgcc aacatcggcg
    21661 ccatgtccac ctggtatccg gtcgccaaga acgacaacct cagttttacc gcgaacacgc
    21721 cgtcgctgtt caccatgccg gcgggcgatg tcggcttcgc cggcgcgatc gagtacggca
    21781 gccagtccta caagatcaac cccgatccgc tagcgctcac cgcggacgcc tactacggcc
    21841 cgcgctacgg cgatggccgc ggcagccgcg accactggag cgccgcgggc gagttgcgcg
    21901 tgccgctgtt gcagtcgctg caggccagcg tggccggccg ctacgacatg tacagctacg
    21961 gcgacaagga tccgggcaaa ttcacctaca gcatgggcct ggagtggcgt ccgctggata
    22021 cgttgctggt gcgcggctcc tatggcaccg gcttccgcgc gccggacatg cactacctgt
    22081 tcgccgacaa cgactactac cgcaccgtct ccaccgacta ctaccagtgc cgcaccgagg
    22141 agccgggcta cagcgacgcc gactgctacg acgacggcag ctgggacgtg aacacgttcg
    22201 acgtctacac cggcaacatg cagctggacg tggaaaccag caagtcgttc accgccggct
    22261 tcgtgtggtc gccgaccgcc aacttcgacc tggcggtgga ctactaccgc atccgcatcg
    22321 ccaaccaggt gcaggcgcag agccgggagc ggctgcgtgc cgacgaggcc aactgcctgc
    22381 tcggcgtgac cgatgccggc gtggccgtcg acatcaactc gccaacctgc gtggatgcgt
    22441 tggcccgcgt gatccgcgag aaccccaacg atccctacag caccatcacc agcgtccact
    22501 tcgccccgat caacatcgcc aacgaagaga cgtcgggcat cgacgtcacc gccaactacc
    22561 gcctgcagag cgccagcgcc ggcgatttcc gcttcaccgg caactacacc tgggcggatc
    22621 gccacagccg ccagcagtat ccaggcgatc cggaagaaga catgctggac ctgagcttca
    22681 gcgccaccac cctgccgcgc accaagggca acctcggcgt ggactgggac aggaatgcgt
    22741 ggggcgcggg cctgttcggc aactacgtgg ggcgcgtcgc caactacaac aacgacgtct
    22801 ggacgcccgc cacctggcgc ttcaacggca gcgcgcgata cgacatcagc gaccacctgc
    22861 gggtctcgct gtcggtcaac aacctgctcg acaagatgcc gccgaaggac gcgacctggg
    22921 cgaactaccc ctactacgac acctcgtggt tcgacagcat gggccgcagc tactacctgc
    22981 agctgacctg gaagctgggc ggcgccccgc tgtaagcggg acccggagcg cgcacgcaaa
    23041 gccccggcat gccggggctc cttgcttgcc gggcgggtcg ttcagtccgc gggctgcacc
    23101 acgtcgtggt tgcggccctt gaacggcttg taccagcgct ggatgcgggc gatgtcggca
    23161 tgcatgtcgt cggtcagctc gaacggcggg ccgatcccga tcaccttgtt cgcgtagtcg
    23221 aaataggccg gcaccaccgg caccccggcg tcgtgggcga tgtgccagaa ccccggccgc
    23281 cagcgctcca cccgcttgcg ggtaccttcc ggggccatcg cgtaccacat gcggtcggcg
    23341 ctgcggatcg cctcgacggc ctggccgacc accccgtgcg gcgagctgcg gtccaccggg
    23401 atgaccccct gccagcgcag cagcgcgcgc agcagcggga cccgcatcag gctgtccttg
    23461 ccgaggatcg acagcttcac gcccatcccg aacttgacgg caaagcccag caagccgtcc
    23521 cagttcgagg aatgcggggc ggcgatcagc accagccgcg gcaggtccgg gaacgcgcct
    23581 gccatgcgcc agccgaacag ccgcaggatc ccgcggccca gcccctccat caggcggttg
    23641 cgcggcatcc gtggcgcgcc cggcggcagc gccagcacca cgtcgttgtc tcgcgcgtcg
    23701 ctcaatccca gtcccgcccc gtgcgcccct tcttgatcgt cgcacgtccg cgtttctcac
    23761 ccaagcggcg ctccctggcc ccgcgcgtcg gcttggtcgc gaccctcggc ttcggcgcgt
    23821 gcaggccgcc ctcgatgaaa tgcgccaggc gttcgcgcgc gtcctggcgg ttgcgctcct
    23881 gggtgcggaa gcgttgcgcg gcgagcacca gcacgccgtc gtcggtgacg cggcggtcgc
    23941 gccgggccag caggcgcagc cgcaccggct ccggcagcga cggcgagcgc gcgatgtcga
    24001 agcgcagctc caccgcggtg gccaccttgt tgacgttctg cccacccggc cccgacgcgc
    24061 gcacgaagcg ttcgatcagt tcgtcgtcgt cgatggcgat ggatccgacc tgcagcatcg
    24121 cgcgattcta acgggctcgc ctcatgcggg agcggaccct gcccgcgacc gcatccttcc
    24181 gagaacgagc gggcgcggac atggttcgct cctacagggg tctgtcgaac agcgccagcg
    24241 ccctggcgaa ttcggcatcg accggcgcgg ccacgtcgag gcgcgcgccg gtgtgcgggt
    24301 gcacgaatgc cagccgccgc gcatgcagca acatccgatg gatgccgagc atccggaaat
    24361 tgcggttgtg gcggccatcg ccatggctgc tgtcgccgat gaggtggtgc gagaggtgct
    24421 tcaggtgcct gcggatctgg cggaagcggc cggtcagcgg cgatgcctgc agcagcgcat
    24481 agcgcgaggt ctcgaatccc gccgaaggca ccggcaactc gcacgtggca agcacctcga
    24541 acgcggtctg cgccggcttc ttcagcggct tgccggggcc gccgtcgagc ggatggtcga
    24601 ccacgaagcg ttgctccgcc ggccagccac ggcataccgc ccagtagtcc ttcgccacct
    24661 cacccgacat cagggtcttg ccgagcgcgg acgcggtctc gcggtcgaac gccagcagca
    24721 ggcagccgct ggtggcgcgg tccagccggt gcaccaggaa gatcggcttg ccgaactgct
    24781 cgcgcaggcg atccgcggcg aaatcggttt caccacgcgc cagcgcgctg tcgtgggcca
    24841 tcagcccggc tggtttgtcg acgacggcaa gtgcttcgtc ttggtacagc accggcaggg
    24901 atgacgaatc ggacatgggt ggcctgcatc gcttcgctag actgccgcca ttgtcccgca
    24961 ccggaacgga ttccatgacc catcaccgcc tgcacgccct cgccctcgcc tgcctgctcg
    25021 ccatgccggc ttccgccttt gccgcgggtg tcgcacctcc cacgggcatc agcgccggcg
    25081 cctgcgtgga aggtatctgc gaataccggc tgcggaacgg cctgcgcgtg ctgctgttcc
    25141 ccgatgccag caagcccacg gtcaccgtca acctgaccta cctggtcggc tcggtgcacg
    25201 aaaactacgg cgaaaccggc atggcccacc tgctggagca cctgctgttc aagggcacgc
    25261 cgaagcatgc ggacatcccc ggcgaactga agaagcgcgg cgtgggcttc aatgccagca
    25321 cctcgctgga tcgcacgaat tacttcagca gcttcccggc caacgacgcc acgctcgact
    25381 acgtgctcgg cctggaagcc gaccgcatgc tcaattcctt cgtcgccaag aaggacctgg
    25441 acagcgagat gaccgtggtc cgcaacgagc tggaacgcaa cgagaacaat cccggcagcg
    25501 tgctcaacga acgcctgcgt tcaacggcct atctgtggca caactacggc aacaccacca
    25561 tcggcgcgcg cagcgacgtc gaacgcgtgc cgatcgaacg cctgcaggcc ttctacaaga
    25621 cctggtacca gccggacaac gcggtgctga tcgtcgccgg ccggttcgat cccgccacgc
    25681 tgctgccaaa catcaaggtg aagttcggcg gcctggggaa gccgtcgcgc gcgctgccgg
    25741 tggcgtacac caaggagccc gcgcaggacg gcgagcgcga agtcaccgtg cgtcgcagcg
    25801 gcgacatccg catcacctcg ctggcctatc acatgcctgc cgccacccat ccggacacgc
    25861 cggcgctggt ggtgctcggc aacctgctgg gccatgcgcc cggcgggcgc ctgcacaagg
    25921 cgctggtgga atccaagctc gccgcgttcg ccggcgcggg cgcgggcgcg atgcgcgacc
    25981 ccggcacctt caacttcgtg gtcgcggtgc cgaaggatgg cgatgccggc aaggccgaag
    26041 cggagttgct gaagcaggcg gaagccatcg cgtcgcaacc agtcaccgcg caggaacttg
    26101 ccgatgccag gcagcgcatc gccaacggtt acgaactggc cttcaacaac gtcaatgcgg
    26161 tgggcatggc cctgtccgag tacgtggccg ccggcgactg gcgattgtgg ttcgtgctgc
    26221 gcgatgcgac ggacaaggtg accctggacg acgtgaaccg ggtggccaag gcctacctgc
    26281 tgcccagcaa ccgcacgctg gcgcgcttcg ttcccaccga caatgcggtg cgcgcgacca
    26341 tcgccgaagc accgcccgcg gccagcctgg tcgagggcta caccggccgc gccgcgatcg
    26401 aagccggcga gtctttcgat ccgaccccgg cgaacatcgc cgcacgcacc gagacctaca
    26461 ccatcggcaa cagcttgacg gtttcgctgc tgccgaagaa gacccgtggc ggtaccgtgg
    26521 tgttcaacgc cgacttccgc ttcggcgacc t
//
LOCUS       DAORUK010000008        25709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_1471_length_25709_cov_17.433110, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000008 DAORUK010000000
VERSION     DAORUK010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 25709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25709
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1471_length_25709_cov_17.433110"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="PK818_01150"
     CDS             <1..1188
                     /locus_tag="PK818_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HQY81374.1"
                     /translation="WLAGAALLHSQAVTEKRGSFGGWTLLLAIAAFSLSLLGTFLVRS
                     GVLTSVHAFAADPTRGLFVLVFLGVVIGGSLLLYALRAPDADANAKPFAASSRETLLL
                     LNNLLLTTACAMVLLGTLYPLLADALELGKISVGPPYFSLMFLLLMAPLVALLPLGPL
                     TRWQREEPASPLRMLLPWAVLAVGAGVVAFLLAPQGQWKAAAGITGALWVGLGTLRFL
                     WSRLGSGKQGSQGTRLTREMLGMVLAHLGVAVFLVGALLVEGLSQQRELAVKPGQTLE
                     LGRHAFRFDGVAEASGPNYTADRGTVQVLRDGRDVTTLHPEKRRYAAGGQVMTESGIA
                     SGVFGDVYVALGESLGGEAWALRVHTKPFVAWIWFGALLMALGAFVTAADRRFRPSRR
                     EVA"
     gene            1191..1730
                     /locus_tag="PK818_01155"
     CDS             1191..1730
                     /locus_tag="PK818_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HQY81375.1"
                     /translation="MSRWLPLALFALLAALLAAGVWMSRKPDRDALPSPLLGKPAPAF
                     ALPVLHEPQRTVTLEQLRGQPFLLNVWGSWCVECRVEHPVLTRFAETRRLRVVGYNWK
                     DEPADALRWLEQYGNPFWLVLADVEGRSAIDWGIYGAPETFLVDRNGVVLWKHVGALT
                     DEIIAGELMPALQKSEGAR"
     gene            1727..2167
                     /locus_tag="PK818_01160"
     CDS             1727..2167
                     /locus_tag="PK818_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HQY81376.1"
                     /translation="MMRWLLAVLLWVATVPAFAQANQQADAAPLQFRDAAEETRFHAL
                     ASELRCVMCQNQSLADSNALIALELRREVLDLMREGKRDGEIKDFLVQRYGEFVLYQP
                     RVAGRTWLLWLGPALLLLAGAGVVTAIVRKRTPPGLPADDGQEW"
     gene            2167..3171
                     /locus_tag="PK818_01165"
     CDS             2167..3171
                     /locus_tag="PK818_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY81377.1"
                     /translation="MNVFLALSALLALAVLLVLLRPLWRDARGVAVGVAVVVLASTGL
                     LYRMVGTPPALDPANLKAPETLGDAIAQLEAELQRNPGQAEGWQLLGQAYQRDNRPAK
                     ARDAFARAVKLSPQSADLATEAAQARALADDRRLFDAEAVALLQGALQVEPEHQRARW
                     FLGIAQRQAGDNAAAAATWEPLLARVDAATAASLRREIDNARIAAGMPPLPGAEPAAP
                     ASGLQVKVALAPGFAERVRLRGDTSVFVIARATDGPPMPVAVEKRRVSELPFTATLDD
                     GDSPMPAGKLSQQREVELVARLSESGEGNRQDGDIESKPVRVVLPAASPVELVIGNPR
                     "
     gene            3305..4417
                     /locus_tag="PK818_01170"
     CDS             3305..4417
                     /locus_tag="PK818_01170"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HQY81378.1"
                     /translation="MTEFIPPGTRWFDLPSPFPMKRGGALHGARIAYETWGALDATAS
                     NAILIVTGLSPDAHAASNAGDPEPGWWEAMLGPGKPIDTDRWFVLCVNSLGSCKGSTG
                     PASTNPDTGQTYRLDFPELSVEDGADAAAAVVRGLGIERLACVIGNSMGGMTALALLQ
                     RHPGIARSHVNISGAARALPFSIAIRSLQREAIRLDPNWNNGDYDDATYPESGMRMAR
                     KLGVITYRSALEWDGRFGRVRLESDRRDDDAFGLEFEVESYLEGHARRFVRRFDPNCY
                     LYLSRSMDWFDAGEHCHGDVLHGLASIRIDRALSIGVRTDILFPLQQQEEIAEGLRAG
                     GCDAAFLALESPQGHDAFLVDIERFGPAVRRFLQAL"
     gene            4555..4640
                     /locus_tag="PK818_01175"
     tRNA            4555..4640
                     /locus_tag="PK818_01175"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4588..4590,aa:Leu,seq:caa)
     gene            complement(4889..5362)
                     /locus_tag="PK818_01180"
     CDS             complement(4889..5362)
                     /locus_tag="PK818_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81379.1"
                     /translation="MQADIGSATARRWVLRVLQALGVAWALPYTALGLVAGAASLAAG
                     AHAHLSARGFAVVFHKVPWGPGGALTLGNVILHSGDSLESPCATYAHRAGHRVEPSIV
                     IGDHERAHVYQYMVLGPLFLPLYFLFGGIGVRNRFERAADRYALTGRGWWPWPRP"
     gene            complement(5365..6441)
                     /locus_tag="PK818_01185"
     CDS             complement(5365..6441)
                     /locus_tag="PK818_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HQY81380.1"
                     /translation="MDAFGPLNITAESLALEADTIRRKLEAGIGTLRAMDPVDFGVTP
                     REEVWRDGKTVLYRFRGDRAPTAKVPLLVCYALVNRPYMVDLQADRSLVKGLLERGED
                     VYIIDWGYPDRSDRFLTLEDYIERFIGGAVDHLRKAHALDAINLLGICQGGVFSLCYA
                     ALHPGKVRNLVTMVTPVDFHTPDNMLSNWNREIDVDLLVDTLGNIPADMMNLTYLMLK
                     PWRLFAQKYVGMVDILDDRKAMEDFLRMEKWIFDSPDQAGEAFREFDTQFFQGNGFVN
                     GGVRIGDEEVHLGLVEMPILNIFAEQDHLVPPDASRALRDLAGTRDYTELSFKGGHIG
                     IYVSGRAQKEVPQTIHEWLAKRSR"
     gene            complement(6443..7495)
                     /gene="phaE"
                     /locus_tag="PK818_01190"
     CDS             complement(6443..7495)
                     /gene="phaE"
                     /locus_tag="PK818_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaE"
                     /protein_id="HQY81381.1"
                     /translation="MAQGSNDFEALARQYWGMWGDAVRGAIPQAGADAGVQGFRDALG
                     AWTSMAGGGNGGMDAVLGHFNRQNSDWFAQMQQVAAQFAGREHSAQDVARAWQQMLGG
                     NPFQGLMHGMRGPGLQDLEQWSQSAAPWLHNLRSEAASTLGMPAFGFAREHQERLQAL
                     GQAQLRWQDALSEYNSLMAKTSQDAYARFESKLAEREEPGRQLGSVRALFDLWVDAAE
                     EAYAASALSLEYRKAYGELVNAQMQLRSRAQVIAEQASMQLGMPGRTELDSAHRKIAE
                     LERQLRRLQRKAEAAEPTAVVTAAGKPASKAKPAKKTAKGAAKKAAKPVATRAAGKAV
                     KKATARKPAKPAARKR"
     gene            complement(7563..7658)
                     /locus_tag="PK818_01195"
                     /pseudo
     CDS             complement(7563..7658)
                     /locus_tag="PK818_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017172041.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
     gene            complement(7683..9470)
                     /locus_tag="PK818_01200"
     CDS             complement(7683..9470)
                     /locus_tag="PK818_01200"
                     /inference="COORDINATES: protein motif:HMM:NF022073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HQY81382.1"
                     /translation="MNILRHGACAAAIALLSACAGVPASSGNPSMFSQQRTTEHRDGD
                     DLLTAGLGLEGLRAMLPPAFADADHPTPAELRRRALWSNWRGIADLAPGGGYGEVYGS
                     VASVPGREFSAFAQVPGATQPHRVLLQVPDAFDAKKRCIVVAASSGSRGIHGAIAVAG
                     AWGLPKGCAVAYTDKGAGTDYFDLDAGQGITADGTAAATDAKLAFKPGATSGTGVAYK
                     HAHSQDNPEADWGRHVKQAAQFALESLDEALPAQAPFTFGNTRVIAVGISNGGGAVLR
                     AAEDGDDWLDAVVAGEPNVLVGGHGARNLFDYSSDAALLMPCALPQLGIPAMPGLEAK
                     CAALAVRGVLAGDTFEARQQDALAKLRAGGWTDAALRAGSISVAFDLWRAVGVGYASA
                     YGRYPHDAHPCGYRYSAMNPDLSVRAATMAERAAWVADASGIPPGAGVGIIDPQAPAD
                     MGLTGLQCLRALWTADSADARRVRKGIEETRAGLPRKGLPVVVIHGTDDGLVPQAFSS
                     APYVAMAKSAGRDVRYWQVRNVQHFDAFLGFPQYGAFYLPLLPYVYNALDRVDAYLDG
                     NGTLPADAVISTIPRAGKPLAGDNLAMPR"
     gene            9592..10416
                     /locus_tag="PK818_01205"
     CDS             9592..10416
                     /locus_tag="PK818_01205"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HQY81383.1"
                     /translation="MNDPTHRDTFLQGRTALVTGSTSGIGLAIATALAAAGSKVAING
                     LGTPEQVASAIATVDAAGNGGTRHFGADLRDADAIAAMMADLDAWSNGGIDILVNNAG
                     IQHAVPLHEMPVSRWNDIIAVNLSSAFHTMRAAMPAMAARGYGRVVNIASVHGLVASK
                     DKAPYVASKFGIVGLSKVAALEYAAQGSRDSGGITVNCVCPGWVETPLIEPQIEARMA
                     GGSRDDGVRALLAEKQPSLRMSLPAEIAALAVFLCRREAHNITGASLPVDGGWTAQ"
     gene            10427..11092
                     /locus_tag="PK818_01210"
     CDS             10427..11092
                     /locus_tag="PK818_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012511122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQY81384.1"
                     /translation="MPTLVIADDHPLFRAALRQAARDALGEVELFEAGTLEAVLALLD
                     AQPQVDLVLLDLHMPGNHGLAGLAAVRAQYPGVAVIVISANDDPRVIRRALDHGAAGY
                     LPKSAGLDDLRDAIRCVLACDTWLPPALRASVTRAQSSPNDAALAARLASLSPQQFRV
                     LVLVAEGLLNKQIADRLDVQERTVKAHLTAIFERLGVRNRTQAGVVLRELELSDPARQ
                     IEG"
     gene            complement(11112..11609)
                     /locus_tag="PK818_01215"
     CDS             complement(11112..11609)
                     /locus_tag="PK818_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HQY81385.1"
                     /translation="MDALKYLAGYPPALQLQVRELLAQGRVGPMLAERYGEPHAVRND
                     GQLYAYVQALKDRHLRKSVPLGKVAYDGKLQVMKHALGTHAATSRVHGGRLKASREIR
                     IASVFRDAPAAFLKMIVVHELAHLKEFEHNKAFYQLCTHMVPDYHQLEFDLRLYLTHL
                     EAKPA"
     gene            11675..12229
                     /locus_tag="PK818_01220"
     CDS             11675..12229
                     /locus_tag="PK818_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012480957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HQY81386.1"
                     /translation="MAKPNPLQEQLLKAGLVKKSKLAEVAREQHKAKHARAASGPSEI
                     ALEAERARAGKAERDRALSLERKAQARTMELRAQARQIIEDKKVAGAGDSEYRFTADG
                     AIRAILVSDDQRRQLSSGLLVIVRHGERHELLPRAAGDKVRERDPGMVVLDHGLGADA
                     GPAPASSDDDAYYAQFQVPDDLVW"
     gene            complement(12246..15602)
                     /locus_tag="PK818_01225"
     CDS             complement(12246..15602)
                     /locus_tag="PK818_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HQY81387.1"
                     /translation="MLSVAAVVAAALAWLGLLFGTGLYAERHPGVFAKHWRHVYALSL
                     AVHCTSWTFYGTVTQAARYGWPLPPTFIGAILCYALAVFFMIRLVRLARESNATSLAD
                     LIATRLGKDAWLAATVTLVAVLGLIPYIALQLQAITMSFSTLTSGAGAAGQGAPPVLR
                     DGALYVALAMALFAILFGTRRASAAEHNRGLVLAMAFESLFKLLAMLVLGAFVWFGLD
                     TLPAVPHAQAAAPAGGFVPLVILGALAMFLMPHQFHVGVVECRDDRDVRTARWQFPLY
                     LLLIALPALPLARAGASLFGERVPSDMYALALPLAQGNQGVALLVFLGGLSAATGMVI
                     VSTLTLSLMIGNHWFAPGLLRGAWARGIGDDGSGDHRGDLLLLRRAGIIAIMLLGWAY
                     SRLVSGNEALADVGAVSFSALATLAPALAFAVWRPQTPANAASFGVLAGFAAWSWVML
                     VPLIAATIGADPAWLREGPFGMHWLAPEALFGLTGWSRLGRAVGVSLFVGSATTVLLA
                     SLRGTPRRREARGSDLLTLRNAGRRFLPAQRVDELLRAAPAKGPVPAVIESRLEHELA
                     AILGSASARLLLDAARRDAAAVDRSGADLDTVAAIVGEASEDLRFNQRVLEAALENMS
                     QGISVVDADLRLVAWNGRYAELFGFPPGMLRVGLPIAEASAWALREVAGIDRHGDALQ
                     RRLEHMRAGTTHLSERVFPDGSIIEIRGNPMPGGGFVATFTDVTAFRQAEAGLMRVNE
                     TLEQRVAERTVLLETAKREAEHANDAKSRFLAAIGHDLLQPLHAAHLFADTLQQRGDG
                     EQRALAVQIGGALDSTTDLLSTLLDMSRLEAGGLVPEPRPFPLADVLDPLVAQFGVMA
                     AERGLRLRFVATRAWVRSDPQLLRRVLQNFLANALRYTPSGSVLLGVRRHGDRLRIEV
                     HDTGPGIDADQREAIFEEFRRGDDAPGQGLGLGLAIAQRIAGLLGSAVLLRSAPGHGS
                     TFAIEVPRASAAHPSHAAPRGLAGLRVLVVDNERQALDALAGVLRGWGCDVVAVADGD
                     AAAKALAARACALWIFDYHLDAGDDGVSLHARLAGRFGNTPCLILSADQTGAVRVAAQ
                     EAGLPLLVKPLRPLALKSVLDRMLAARAVA"
     gene            complement(15720..17948)
                     /locus_tag="PK818_01230"
     CDS             complement(15720..17948)
                     /locus_tag="PK818_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012480328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY81388.1"
                     /translation="MKRKQLSLAIHGVLAVSLLATMPVMAQDAAAADAGGQKTTTLES
                     IKVTARKREETLQEVPVAVTAFTADTLDKLNVRDLSDLDAQVPNLTIYAARGSNSTLT
                     AYIRGVGQSDPLWGVDPGVGLYMDDVYIARPQGALLDVFDVERVEVLRGPQGTLYGKN
                     TIGGAIKYISRGLRSDFNGFGSLTIGNYGQRDIKAAVGGGLGGSEYLRGRISIADLHN
                     DGFGENIVTGDPVSDKDVTAYRLSLGAYVTDNFDLQFALDHMKDGSGVRGAQMLQANR
                     NVPNTPPLESRYDVRNGMPNVNETTMSGASVTANLRASDDWTFKYVLAKRESDTETNI
                     DFDVTQSKIADVKAFYDDEQVTHELQAMYDGGGRSRGVMGVYMFDGYAGGQVLNNFFN
                     FLFGDTQGNVYTESYAAYADWTFDVTEQFKVDVGARYTNEEKHAVVLNRGYSDATYTR
                     PISVAADFDKTISFNNFSPKISLDYQVTPDILVYGLASRGFKSGGYNIRAQATAVPRS
                     AEPFDDEQVDSYEAGTKMGLLDGSLFLNLSAFHNKYKDIQLSVFTAYDSNGDGTEDAF
                     FGDFTNAGSGTVNGFEVEYQWLPSQHWLVSGNLAWLDTEYEEFMFAGVNIADEQEFTN
                     APDFSGALNVEYRTPVGAGDISARIGYSYQASVVATTEINRAEVPPNGRPPISQDGYG
                     LLNAGVIWNINPAWTVSLQGTNLTDKQYLTTGYNLNRALGVYTGFYGPPRQYSLSVRY
                     NF"
     gene            18110..19513
                     /locus_tag="PK818_01235"
     CDS             18110..19513
                     /locus_tag="PK818_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HQY81389.1"
                     /translation="MAFLIVLAALCFLMFVAYRGYSVILFAPVAALGAVLLTDPQLVA
                     PMFTGLFMDKMVGFLKLYFPVFLLGALFGKVIELSGFSKSIVAATIRVVGAQRAMLSI
                     VLVCALLTYGGVSLFVVVFAVYPFAAELFRQSDIPKRLIPGTIALGAFTFTMDALPGT
                     PQIQNIIPTTFFGTDTWAAPVLGTLGGIFILILGMLYLESRRRAALEAGEGYGDAASL
                     FNEPVPFAGGKLANPAVAILPLLVVGIVNKLLTLWIPQHYGASHSFDPAVIGSAAPVV
                     QEVSKVAAIWAVEGALLAGIACVLLFAWKPVTTSFAEGSRSAISGALLAGMNTASEYG
                     FGAVIAALPGFLVVANALGSIPNPLVNEAVTVTALAGITGSASGGMSIALAAMAETFI
                     ANANAAGIPMEVLHRVAAMASGGMDTLPHNGAVITLLAVTGLTHRQAYKDIFAITCIK
                     TMAVFVVIGLYYAFGLT"
     gene            19557..20846
                     /locus_tag="PK818_01240"
     CDS             19557..20846
                     /locus_tag="PK818_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81390.1"
                     /translation="MDARRPPALSARTTPTWEVELLISGVAVFAMLQLPGWLDDRMFA
                     LEPRVGSDWRPVLLLFYIYSKSTAVVLAVTFAIHLLLRAQWIALVGMDSVYPQGVQLD
                     RLRMGPIQRAIEEARPESSADAIERADNRASVLFAIGVSIALIIASICIFFCGSLLLV
                     NLLAQSFDWPIDPLKVMLGLFGALMLPFMAASMIDQFRGDRLRPGGATHRVIAATLRF
                     YTRIGMGRRNSHVVALLTSNGGERRMMAVVVGIMVAALLGVGAAYTAMGSPGAIGSYA
                     LFPDSGHLQLRSEHYDDQRNPARDAALPFVQSAVITGPYVKLLVPYRPDRDQPAMRTH
                     CAHARLLQGDALATARLACLQALHPVSLDGKPLPGLRYEIASDPRTDRPALLAMIDVR
                     NLAPGRHDLRIARPPRGDRRPDNDNPDPGFDAIAFWR"
     gene            20878..21993
                     /gene="dinB"
                     /locus_tag="PK818_01245"
     CDS             20878..21993
                     /gene="dinB"
                     /locus_tag="PK818_01245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HQY81391.1"
                     /translation="MSEAPQRKIIHVDMDAFYASVEQRDDPSLRGKPVVVAWRGARSV
                     VCAASYEARVFGVRSAMPAVRAERLCPQAVFVPPDFLRYKAVSRQVRGIFERHTNLIE
                     PLSLDEAYLDVSAPKHDLGSATAIASSIRDAIREETRLTASAGIAPNKFLAKIASDWN
                     KPDGQFVLKPAMVDAFLAPLAVGKLPGVGKVMEAKLAELGIATCADLRAFGDDALERR
                     FGRWGRRLHELSLGIDERAVQPGRPTLQVSAEDTFEQDLPLTALELHIRRLAAKAWAG
                     YLRERQRHPERIARTVVLKLKTADFRILTRSLTANAPPACETAFADAACGLRERVGLP
                     AGTRFRLAGVGLAGFVDADLARAQDDLFAGARGSRDG"
     gene            22157..23245
                     /locus_tag="PK818_01250"
     CDS             22157..23245
                     /locus_tag="PK818_01250"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81392.1"
                     /translation="MTATTDLAEGVHAGILGRPGPAGGSGASTRGIPRIAYQGVRMTA
                     NLVGGALDASLARLTPWLGPSAGSSRRDALVAVLNGVLGDHLAASANPLALEARLRVG
                     GRALPLRGEDWPSLPLAPTPRLLVQVHGLCMNHLQWRRQGHDHGERLAAELGYTPVHL
                     HYNSGLSIESNGRTFATLLQQLIQDWPVPVERFAILGHSMGGLVARAAIASALQERQQ
                     WLERLDQLVFLGTPHHGAPLERAGHVLQSVLGLTPWTAPFMRLGEVRSAGIQDLRHGL
                     GEDLPLPPGVRAYAVAASLQGVRAGHDHRRLRGDGLVPVRSAFGDHRDPLRALGIPEA
                     HRLLVERTGHLDLLNSHAVYATLRHWLA"
     gene            23285..24565
                     /locus_tag="PK818_01255"
     CDS             23285..24565
                     /locus_tag="PK818_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017158056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY81393.1"
                     /translation="MTPHATPALSRAQLKTLALASLGAALEFYDFVIFVFFAATMGAL
                     FFPADMPEWLRLVQTFGIFAAGYLARPLGGIVMAHFGDLSGRKRMFMLSILMMAIPTL
                     LMGLLPTYAQAGVLAPILLLLLRIMQGAAIGGEAPGAWVFVSEHVPPRHRTLACGALS
                     AGLCSGILIGSLVARATDAMFDQAEVLAWGWRLPFIVGGVFGLLAMVLRRQLHETPVF
                     TELRARRALAAELPLKTVVRDHAGAVLLAMLLTWLLTAAVVVTLLMMPTLLQGLGVSR
                     ADALAGNTLAIFATVLANLAAGWLADRFGPGRVLVLWSASLGAAFWLFYGNALAGAYS
                     PALYVVAGASVGLTALVPALAVGGFPPQVRFSGLSFAYNLAYAIAGGLTPVLLSLAIR
                     DNPAAPMQYIAAMAVLGIALGLYLSVRPPRHGAR"
     gene            24582..24782
                     /locus_tag="PK818_01260"
     CDS             24582..24782
                     /locus_tag="PK818_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HQY81394.1"
                     /translation="MHSQRPGPDPDPRPQPPQRPLPTDCCDSGCPVCVHDLYADEVAA
                     YAKALADWNQRHPDPGGSPPAA"
     gene            complement(24874..>25709)
                     /locus_tag="PK818_01265"
     CDS             complement(24874..>25709)
                     /locus_tag="PK818_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011259733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HQY81395.1"
                     /translation="TIRVVSEITESNGSSSMASVCGSSLALMDAGVPVKAPVAGIAMG
                     LVKEGDDFVVLSDILGDEDHLGDMDFKVAGTSNGISALQMDIKIQGITEEIMKVALAQ
                     AKAGRLHILGEMASALTAPRAELSDFAPRLITIKIHPDKIREVIGKGGSVIQAITKET
                     GTQIDIQDDGTITIASVNAAAGQAAKARIEQITSDVEPGRIYEGKVAKLMDFGAFVTI
                     SPGKDGLVHVSQISNDRVEKVSDVLKEGDIVKVKVLEVDKQGRIRLSMKAVAEGEGTA
                     E"
BASE COUNT         3763 a         9006 c         8860 g         4080 t
ORIGIN      
        1 tggctggccg gcgcggcgct gctgcattcg caggcggtga ccgagaagcg cggcagcttc
       61 ggcggctgga ccctgctgct tgcgatcgcg gcgttctcgc tgtcgctgct tggcaccttc
      121 ctggtgcgct cgggggtgct gacctcggtg catgcgttcg ccgcagaccc gacccgcggc
      181 ctgttcgtgc tggtgttcct cggcgtcgtg atcggcggct cgctgctgct gtacgcactg
      241 cgtgcacccg atgccgatgc gaacgcgaag ccctttgccg ccagttcgcg cgaaaccctg
      301 ctgctgctca acaacctgct gctgaccacg gcctgcgcga tggtgctgct gggcacgctg
      361 tacccgctgc tggcggatgc gctggagctg ggcaagatct cggtcggccc gccctatttc
      421 tcgctgatgt tcctgctgct gatggcgccg ctggtcgcgt tgctgccgct cgggccgctg
      481 acccgctggc agcgcgagga gcccgcctcg ccactgcgca tgctgctgcc gtgggcggtg
      541 ctggcggtgg gtgccggcgt ggtcgcgttc ttgctggcgc cgcagggcca gtggaaggcc
      601 gccgccggca tcaccggcgc gctttgggtc ggcctcggca ccctgcgctt cctgtggtcc
      661 aggcttggca gcggcaagca gggttcgcaa ggcacgcgcc tcacgcgcga aatgctcggg
      721 atggtgctgg cgcacctggg cgtcgccgtt ttcctggtcg gcgcattgct ggtggaaggc
      781 ctgagccagc agcgcgagct ggcggtgaaa ccggggcaga cgctcgaact cggccggcat
      841 gcattccgtt tcgatggcgt cgccgaggcc agtggcccca attacaccgc cgatcgcggc
      901 acggtgcagg tcctgcgcga cggccgcgac gtcaccaccc tgcacccgga gaagcgtcgc
      961 tacgcggccg gtggccaggt gatgaccgaa tccgggatcg cctccggcgt gttcggtgac
     1021 gtctacgtcg cgcttggcga atcgcttggc ggcgaggcat gggcgctgcg cgtgcacacc
     1081 aagccgttcg tggcctggat ctggttcggc gcgttgttga tggcgctggg cgcgttcgtc
     1141 accgccgccg atcgtcgctt ccgcccctcg cgccgggagg ttgcatgaag atgtcgcgct
     1201 ggttgccgct tgcccttttt gcgctgctgg ccgcactgct cgcggccggg gtgtggatga
     1261 gccgcaagcc ggaccgcgat gcgctgccct cgccgctgct gggcaagccg gcgccggcgt
     1321 tcgcgttgcc ggtactgcac gaaccgcagc gcacggtgac cctggagcag ctgcgcggcc
     1381 agccgttcct gctcaacgtg tggggcagct ggtgcgtgga atgccgcgtc gagcacccgg
     1441 tattgacccg cttcgccgag acccgccgcc tgcgcgtggt cggctacaac tggaaggacg
     1501 agcctgcgga tgcactgcgc tggctggagc agtacggcaa tccgttctgg ctggtgctgg
     1561 ccgacgtcga gggccgcagc gcgatcgact ggggcatcta cggcgcgccg gagaccttcc
     1621 tggtggaccg caacggcgtg gtgctctgga agcacgtggg cgcactgacc gacgaaatca
     1681 tcgccggcga actgatgccg gcgctgcaga agagcgaggg cgcgcgctga tgcgctggct
     1741 gctggccgtg ctgctgtggg tcgccaccgt tcccgcgttc gcgcaggcga accagcaggc
     1801 cgacgccgcg cccctgcagt tccgcgatgc cgccgaagaa acccgcttcc atgccctggc
     1861 ctctgagctg cgctgcgtga tgtgccagaa ccagtcgctg gccgattcca acgcgctgat
     1921 cgcgctggaa ctgcgccgcg aagtgctgga cctgatgcgc gaaggcaagc gcgacggcga
     1981 gatcaaggac ttcctggtgc agcgttacgg cgaattcgtg ctgtaccagc cgcgggtcgc
     2041 gggccgcacc tggctgctgt ggctgggtcc ggcgctgctg ctgctggccg gtgcaggcgt
     2101 ggtcacggcg atcgtgcgca agcgaacgcc gccgggactg cccgccgacg atggacagga
     2161 gtggtgatga acgtcttcct tgcgctctcc gcgctgctgg cgctcgccgt gctgctggtg
     2221 ctgctgcggc cgttgtggcg cgatgcgcgt ggcgtcgcgg tcggggtcgc ggtggtcgtg
     2281 ctggcctcga ccggtctgct gtaccgcatg gtcggcaccc cgccggcgct ggatccggcg
     2341 aacctgaagg caccggaaac cctgggcgat gcgatcgcgc agctcgaagc cgagctgcag
     2401 cgcaatccag gccaggccga aggctggcag ttactgggcc aggcctacca gcgcgacaac
     2461 cgccccgcga aggcgcgcga cgcgttcgca cgggcggtca agctttcccc gcagtcggcc
     2521 gatctcgcca ccgaggcggc gcaggcgcgc gcgctggcag acgaccgtcg cctgttcgat
     2581 gccgaggcgg tcgcactgct gcaaggtgcg ctgcaggtgg aacccgagca ccagcgtgca
     2641 cgctggttcc tcggcatcgc gcaacgccag gccggcgaca acgccgccgc cgccgcgacc
     2701 tgggaaccgt tgctggcgcg cgtggacgcc gcgaccgccg ccagccttcg ccgggagatc
     2761 gacaatgccc ggatcgccgc cggcatgccg ccgctgccgg gggcggaacc cgccgcgcct
     2821 gcctccggct tgcaggtgaa ggtcgccctg gcccccggct ttgccgagcg cgtgcgcctg
     2881 cgtggcgaca ccagcgtgtt cgtgattgcg cgcgcaacgg acggaccgcc gatgccggtc
     2941 gcggtcgaaa agcgccgcgt gtccgagttg ccgttcaccg ccacgctcga tgatggcgac
     3001 agcccgatgc cggccggcaa gctgtcgcag cagcgcgagg tcgaactggt cgcgcgcctg
     3061 tccgaaagtg gcgaaggcaa ccgccaggac ggtgacatcg aatcgaagcc ggtgcgcgtc
     3121 gtgttgcccg cagcgtcgcc ggtggagttg gtgatcggca accctcgata agcaccgctg
     3181 ccctgccgtg ggtgtcgcga gcaggccatg gatggcctgc gcccgcgcat cggagcggga
     3241 tgcggagcct aagcggcgcg acacccatgg cagggcagcg gtgcgccccg gatacacttc
     3301 acccatgacc gaattcattc cccctggcac gcgctggttc gacctgccct cgccgtttcc
     3361 gatgaagcgc ggcggcgcgt tgcacggcgc gcgcatcgct tacgagacct ggggcgcgct
     3421 cgatgccacc gccagcaacg cgatcctgat cgtcaccggg ctgtcgccgg acgcgcatgc
     3481 agcctcgaat gccggcgatc cggaaccggg ttggtgggag gccatgctcg gccccggcaa
     3541 gccgatcgac accgaccgct ggttcgtgct gtgcgtgaat tcgctgggca gttgcaaggg
     3601 ctcgaccggg cctgcgtcca ccaatcccga caccgggcag acgtaccggc tcgacttccc
     3661 ggaactgtcg gtggaagacg gcgccgatgc ggcggccgcg gtcgtgcgcg ggctcggcat
     3721 cgagcgcctg gcgtgcgtga tcggcaactc gatgggcggc atgaccgcgc tggcgctgct
     3781 gcagcggcat ccgggcatcg cccgcagcca cgtcaacatc tccggggcgg cgcgcgcgct
     3841 gccgttctcg atcgcgatcc gctcgctgca gcgcgaggcg atccggctgg acccgaactg
     3901 gaacaacggc gactacgacg acgccaccta tccggaatcc ggcatgcgga tggcgcgcaa
     3961 gctgggcgtg atcacctatc gctcggcgct ggaatgggac ggccgcttcg gccgggtgcg
     4021 cctggagtcc gaccgccgcg acgatgatgc cttcggcctg gaattcgagg tcgaaagcta
     4081 cctcgaaggc cacgcccgcc gcttcgtgcg ccgcttcgac ccgaactgct acctctacct
     4141 gagccgctcg atggactggt tcgacgccgg tgagcattgc catggcgacg tgttgcacgg
     4201 gcttgcatcc atccggatcg accgcgcgct ttcgatcggc gtgcgcaccg acatcctgtt
     4261 ccccctgcag cagcaggagg agatcgccga aggcctgcgt gccggcggct gcgacgcggc
     4321 gttcctggcg ctggaatcgc cgcagggcca cgatgccttc ctggtcgaca tcgagcgatt
     4381 cggcccggcg gtgcgcaggt tcctccaagc gctgtgagga ccatgccgcg caagaaggaa
     4441 ccgcatcggc tggatgacca tccagccgcc ggacctgaag tccggtcaag gtggcctggc
     4501 tggccaccga ctggatcgca tcggcgcgcc gctcggctat acttcgcctc ccgcgccggt
     4561 gtggcggaat ggtagacgca gcggactcaa aatccgccgg cagcgatgtc gtggaggttc
     4621 gagtcctctc accggtacca gatgacgaaa gccacgcatc gcgtggcttt ctcgtttgcg
     4681 cgctcttcgc ggggcgcaaa agggaagacc cgatctcgcg gacagctgag tgtctggagg
     4741 ggatcgggcc ttctgtggcc ggagcgattg tccgaggtct ctttcgatcc cgcgaagttt
     4801 ttctccgacg aggggacgct acgcccgcgc ccgttaggaa aaagctaagc cgggcgcatt
     4861 catccgaccg ttcgtcggga ggtgggcgtc agggcctcgg ccacggccac caaccgcgcc
     4921 cggtcagtgc gtagcgatcc gcggcacgct cgaagcggtt gcgcacgccg atcccgccga
     4981 acaggaaata cagcggcagg aacagcgggc ccagcaccat gtactggtag acgtgcgcgc
     5041 gctcgtggtc gccgatcacg atgctgggct cgacgcgatg gccggcgcgg tgcgcatagg
     5101 tcgcgcaagg cgattccagg ctgtcgccgg agtgcaagat cacgttgccc agggtcagcg
     5161 ccccgcccgg cccccatggc accttgtgga acaccaccgc gaagccgcgt gcactgaggt
     5221 gcgcatgcgc gccggccgcg aggctggccg cgcccgcgac cagcccgagc gccgtgtatg
     5281 gcagggccca ggccacgccc agcgcctgca acacgcgcaa cacccagcgc ctggccgttg
     5341 cagacccgat gtccgcctgc atgttcagcg cgaacgcttg gccagccact cgtggatggt
     5401 ctgcggcact tccttctgcg cacgcccgga cacgtagatg ccgatgtggc cgcccttgaa
     5461 cgaaagctcg gtgtagtcgc gggtcccggc gaggtcgcgc aaggcgcgcg aggcgtccgg
     5521 cggcaccagg tggtcctgct cggcgaagat gttgaggatc ggcatttcca ccaggcccag
     5581 gtgcacctcc tcgtcgccga tgcgcacgcc gccgttgacg aagccgttgc cctggaagaa
     5641 ctgggtgtcg aattcgcgga aggcttcgcc agcctggtcg ggcgaatcga agatccactt
     5701 ctccatgcgc aggaaatcct ccatcgcctt gcggtcgtcg aggatgtcga ccatgcccac
     5761 gtacttctgc gcgaacagcc gccacggctt cagcatcagg taggtgaggt tcatcatgtc
     5821 cgcggggatg ttgcccagcg tgtcgaccag caggtcgacg tcgatctcgc ggttccagtt
     5881 cgacagcatg ttgtccgggg tatggaaatc caccggggtg accatggtga ccaggttgcg
     5941 caccttgccg gggtgcagcg ccgcatagca cagcgagaac acgccgccct ggcagatccc
     6001 gagcaggttg atcgcgtcca gcgcgtgggc cttgcgcagg tggtcgaccg caccgccgat
     6061 gaagcgttcg atgtagtcct ccagggtgag gaagcggtcg ctgcggtccg ggtagcccca
     6121 gtcgatgatg tagacgtcct cgccgcgttc cagcagcccc ttcaccagcg agcgatcggc
     6181 ctgcaggtcg accatgtacg gacggttgac cagcgcgtaa cagaccagca gcggcacctt
     6241 cgcggtgggt gcgcgatcgc cgcggaagcg gtacagcacg gtcttgccgt cgcgccagac
     6301 ttcctcgcgc ggggtgacgc cgaaatccac cggatccatt gcacgcaggg tgccgatccc
     6361 ggcctccagc ttgcgccgga tggtgtccgc ttccagcgcc agcgactcgg cggtgatgtt
     6421 gagtggcccg aatgcatcca tgtcagcgct tcctcgcggc gggcttggcc ggcttgcgcg
     6481 cggtcgcctt cttcactgcc tttcctgcag ccctcgtggc gaccggtttc gctgccttct
     6541 tcgccgctcc cttcgccgtt ttcttggcgg gcttcgcctt ggatgccggc ttacctgcag
     6601 cggtaaccac agcggtcggt tcggcagcct cagccttgcg ctggaggcgc cgcagctggc
     6661 gttccagttc cgcgatcttt cgatgcgcgc tgtcgagctc ggtgcggccc ggcatcccca
     6721 gttgcatcga cgcctgctcg gcgatcacct gcgcgcgcga acgcaactgc atctgcgcat
     6781 tgaccagttc gccgtaggcc ttgcggtact cgagggacag cgcgcttgcg gcataggctt
     6841 cttccgcggc atcgacccag aggtcgaaca gggcgcgcac gctgcccagc tggcgtcccg
     6901 gttcctcgcg ctcggccagc ttcgactcga agcgtgcata cgcgtcctgc gaggtcttcg
     6961 ccatcaggct gttgtactcg gacaacgcat cctgccagcg cagctgcgcc tgccccaggg
     7021 cctgcagccg ctcctggtgc tcgcgggcga acccgaaggc cggcattccg agcgtgctcg
     7081 ccgcttcgct acggaggttg tgcagccacg gcgcggcaga ctggctccac tgctccaggt
     7141 cctgcaggcc ggggccgcgc atgccgtgca tcaggccctg gaacgggttg ccgcccagca
     7201 tctgctgcca ggccctggcc acgtcctgcg cgctgtgctc gcggccggcg aattgcgcgg
     7261 caacctgctg catctgcgcg aaccagtcgc tgttctggcg gttgaaatgc ccgagcacgg
     7321 cgtccatccc gccgtttccg cctccggcca tgctggtcca cgcgccgagt gcatcgcgga
     7381 agccctgcac gcccgcatcg gcacctgcct gcgggatcgc accgcgcacg gcatcgcccc
     7441 acatgcccca gtattggcgc gccagcgcct cgaaatcgtt ggaaccctgt gccatgtgac
     7501 ctccacgcgt tgcagccatg ctagcaatgc ggtcatgacg atgccacgtc aacgcacgcg
     7561 ggtcagcgct ttgggatccg cagggtcttg ctgatcatca gcgaaccgga cagcgcgaac
     7621 accagcacca gcggatgcag ctgccacggc ccgagctggg ccacgcagga ctgcagcagg
     7681 gatcagcgcg gcatcgccag gttgtcgccc gccagtggtt tcccggcgcg cggaatggtg
     7741 ctgatcaccg cgtcggccgg cagcgtgccg ttgccgtcca ggtacgcgtc cacccggtcc
     7801 agtgcgttgt agacgtaggg cagcagcggc aggtagaacg cgccgtactg cgggaagccc
     7861 aggaaggcat cgaagtgctg cacgttgcgg acctgccagt agcgcacgtc gcggcccgcg
     7921 gacttcgcca tcgccacgta cggcgcgctg gagaacgcct gcggcaccag gccgtcgtcg
     7981 gtgccatgga tcaccacgac cggcaggccc ttgcgcggca ggccggcacg ggtctcctcg
     8041 atgcccttgc ggacgcgtcg cgcatccgcg ctgtcggccg tccagagggc acgcaggcac
     8101 tgcaggccgg tgaggcccat gtcggcgggc gcttgcggat cgatgatgcc cacgcctgcg
     8161 ccgggcggaa tcccgcttgc atccgcgacc catgccgcgc gttcggccat ggtggccgcg
     8221 cgcacgctga gatccgggtt catcgcggaa tagcggtaac cgcagggatg cgcgtcatgc
     8281 ggatagcggc cgtaagccga ggcatagccc acgccgaccg cgcgccacag gtcgaaggcc
     8341 acgctgatcg agcctgcgcg cagcgcggca tcggtccagc cgccggcgcg cagcttggcc
     8401 agcgcgtcct gttggcgggc ctcgaaggtg tcgcctgcga gcacgccacg caccgccagt
     8461 gcggcgcatt tggcctccag ccccggcatc gccgggatgc cgagttgcgg caacgcgcag
     8521 ggcatcagca atgcggcatc gctgctgtag tcgaacaggt tgcgcgcgcc gtggcctccc
     8581 accagcacgt tgggttcgcc agccacgacg gcatccagcc aatcatcgcc gtcctccgcc
     8641 gcacgcagca ccgcgccacc gccgttggaa atgccgaccg cgatcacccg cgtgttgccg
     8701 aaggtgaaag gcgcctgcgc cggcagggcc tcatccaagg actccagtgc gaactgcgcc
     8761 gcctgcttca cgtggcggcc ccagtcggct tccgggttgt cctgcgaatg cgcatgcttg
     8821 taggcaacgc cggtgccgct ggtcgcgccc ggcttgaacg ccagcttcgc gtccgtggcc
     8881 gccgccgtgc cgtccgcggt gatgccctgc cccgcatcca ggtcgaagta gtcggtgcct
     8941 gcgcccttgt cggtgtaggc caccgcgcag cccttcggca ggccccacgc acccgcgacg
     9001 gcgatggcgc catggatccc gcgcgatccc gaagaggccg ccaccacgat gcagcgcttc
     9061 ttcgcatcga acgcatccgg cacctgcagc agcacgcggt gcggctgggt cgcgcccggc
     9121 acctgcgcga acgcgctgaa ctcgcggccc gggacgctgg ccacgctgcc gtagacctcg
     9181 ccatagccgc cgcccggggc gaggtcggcg atcccgcgcc agttgctcca cagcgcgcgc
     9241 cggcgcaact cggccggggt tggatgatcg gcgtcggcga acgccggcgg gagcatcgcg
     9301 cgcagcccct ccagcccaag ccctgcggtc agcaggtcgt cgccgtcgcg atgctcggtg
     9361 gtgcgttgct ggctgaacat ggacgggttc ccggaggatg caggcacgcc cgcgcaggcg
     9421 gacaacaggg cgatggcggc ggcgcaggcg ccatgacgga ggatgttcat gcgcggacag
     9481 tacgacgccc gcggccgcgc cgcatcgtac cttcgtacca gtgccggcaa cggcatcctt
     9541 cgccgcggcc tgctagcgta tgccgctggc gcacggcgac atggaaccgc gatgaacgac
     9601 cccacccacc gagacacttt cctgcagggc cgcaccgcac tggtgaccgg cagcacctcc
     9661 ggcatcggcc tcgcgatcgc cactgcgttg gccgcggccg gcagcaaggt tgcgatcaac
     9721 gggctgggca cgccggagca ggtcgcgtcg gcgatcgcca ccgtcgatgc cgcgggcaac
     9781 ggcggcaccc gccactttgg cgccgacctg cgcgatgccg acgcgatcgc ggcgatgatg
     9841 gccgacctcg acgcctggtc gaacggtggc atcgatatcc tggtcaacaa cgccggcatc
     9901 cagcacgcgg tgccgctgca cgagatgccg gtctcgaggt ggaacgacat catcgcggtc
     9961 aacctgtcgt ccgcgttcca caccatgcgc gcggcgatgc cggcgatggc cgcgcgcggc
    10021 tacggccggg tggtcaacat cgcctcggtg cacgggctgg tggcatcgaa ggacaaggcg
    10081 ccgtacgtgg ccagcaagtt cggcatcgtc ggcctcagca aggtcgccgc gctggaatac
    10141 gccgcgcaag ggtcgcgcga ctccggcggg atcaccgtca actgcgtctg ccccggctgg
    10201 gtggagacgc cgctgatcga gccgcagatc gaggcgcgca tggccggcgg ctcgcgcgat
    10261 gacggcgtgc gtgcgctgct ggccgagaag cagcccagcc tgcgcatgag cctgcccgcc
    10321 gagatcgccg cgctggcggt gttcctgtgc cggcgcgagg cgcacaacat caccggcgcc
    10381 tcgctgccgg tggatggcgg ctggaccgcg caataggccc ggcgcgatgc ccaccctcgt
    10441 catcgccgac gaccatccgc tgttccgcgc cgccttgcgg caggccgcgc gcgatgcgct
    10501 gggcgaagtc gagctgttcg aggccggcac cctggaggcg gtgctggccc tgctcgatgc
    10561 gcaaccgcag gtggacctgg tgctgctcga cctgcacatg cccggcaacc acggcctggc
    10621 tggccttgcg gcggtgcgcg cgcagtatcc gggggtcgcg gtgatcgtga tctccgccaa
    10681 cgacgatccg cgcgtgatcc ggcgcgcgct cgaccacggc gccgccggct acctgccgaa
    10741 gagcgccggc ctcgacgacc tgcgcgatgc catccgctgc gtgctcgcct gcgacacctg
    10801 gctgccgccg gcgttgcgtg cctcggtcac gcgcgcgcag agctcgccca acgacgcggc
    10861 gctggccgcg cgcctggcca gcctgtcgcc gcagcagttc cgggtgctgg tgctggtcgc
    10921 cgagggtctg ctcaacaagc agatcgcgga ccgcctggac gtgcaggaac gcacggtcaa
    10981 ggcgcacctg accgcgatct tcgaacgcct gggcgtgcgc aaccgcaccc aggccggggt
    11041 ggtcctgcgc gaactggaac tgtccgaccc ggcgcgccag atcgagggct gagcgcggct
    11101 gccaaccggc ctcaagccgg tttggcctcg aggtgggtca ggtacaggcg caggtcgaac
    11161 tccaactggt ggtagtcggg caccatgtgc gtgcacaact ggtagaacgc cttgttgtgc
    11221 tcgaattcct tcaggtgcgc cagttcgtgc accacgatca tcttcaggaa tgcagcgggg
    11281 gcgtcgcgga acaccgaggc gatgcggatc tcgcggctgg ccttgagcct gccgccgtgc
    11341 acccgcgagg tggcggcgtg ggtgccgagc gcatgcttca tcacctgcag ctttccgtcg
    11401 taggcgacct tgcccagcgg caccgacttg cgcaggtggc gatccttcag cgcctgcacg
    11461 taggcgtaca gctggccgtc gttgcgcacg gcatgcggct cgccgtacct ctccgcaagc
    11521 atggggccaa cgcgtccttg cgcgagcagt tcgcgcacct gcagttgcag ggcgggcgga
    11581 tagccggcga ggtatttcag ggcgtccatg cgtcgaagtg ccggcggcgt tggccgctat
    11641 catggcgccg cgtccaccaa caggaaaccg ggtcatggcc aagccgaatc cactgcagga
    11701 acagttgctc aaggccggcc tggtcaagaa gtccaagctg gccgaggtgg cgcgcgagca
    11761 gcacaaggcc aagcatgcca gggcagcgtc cgggcccagc gagatcgcgc tggaagccga
    11821 gcgtgcacgc gccggcaagg ccgagcgcga ccgcgcgctc tcgctcgaac gcaaggcgca
    11881 ggcgcgcacc atggaactgc gtgcacaggc acggcagatc atcgaggaca agaaagtggc
    11941 cggggccggc gacagcgaat accgcttcac cgccgacggc gcgatccgcg cgatcctggt
    12001 cagcgacgac cagcgcaggc aactgtcctc cggcctgctg gtgatcgtcc gccatggcga
    12061 acgccacgag ctgctgccgc gcgcggccgg cgacaaggtg cgagaacgcg atcccggcat
    12121 ggtcgtgctc gaccacggcc ttggcgcgga cgccgggcct gctcccgcca gctccgacga
    12181 cgacgcctac tacgcgcagt tccaggtacc cgacgacctg gtctggtagc gcgccgggcc
    12241 ctgccctaag ccaccgcccg cgccgccagc atccggtcca gcaccgactt caacgccaag
    12301 ggccgcagcg gcttcaccag cagcggcagg cctgcctcct gcgccgcgac gcgcaccgcg
    12361 ccggtctggt cggcgctgag gatcaggcag ggcgtgttgc cgaagcgacc tgcgaggcgc
    12421 gcatgcaggc tgacgccatc gtcgccggca tcgaggtggt agtcgaagat ccacaacgcg
    12481 caggcgcgcg ccgccagcgc cttcgcagcc gcatcgccgt cggccacggc gaccacgtcg
    12541 cacccccagc cgcgcagcac gccggccagc gcgtccagcg cctgccgctc gttgtcgacc
    12601 accagcaccc gcagcccggc caggccgcgc ggcgcggcat gcgacggatg cgcggcgctg
    12661 gcgcgcggca cctcgatggc gaacgtgctg ccgtggcccg gcgcgctccg caacagcacc
    12721 gcggatccga gcaacccggc gatgcgctgg gcgatggcca ggcccaggcc caggccttgc
    12781 ccgggcgcat cgtcgccgcg gcggaactcc tcgaagatcg cctcgcgctg gtcggcatcg
    12841 atgccggggc cggtatcgtg cacctcgatg cgcagccggt cgccatgccg gcgcacgccc
    12901 agcaacacgc tgcctgatgg cgtgtagcgc aacgcgttcg caaggaagtt ctgcagcacc
    12961 cggcgcagca gttgcgggtc gctgcgaacc catgcccggg tggccacgaa ccgcagccgc
    13021 aggccgcgct ccgcggccat taccccgaac tgcgcgacca gcggatccag cacgtccgcc
    13081 agcgggaacg ggcgcggctc cggcaccagc ccgcccgcct ccagccgcga catgtccagc
    13141 aaggtgctca gcaggtcggt ggtggaatcc agcgcgccac cgatctggac cgccagcgcg
    13201 cgctgctcgc catcgccgcg ctgctgcagg gtgtcggcga acaggtgggc ggcgtgcagc
    13261 ggctgcagca ggtcgtggcc gatcgcggcg aggaagcggc tcttggcatc gttggcatgc
    13321 tccgcttcgc gcttcgcggt ctccagcaac acggtgcgct cggccacccg ctgctccagg
    13381 gtttcgttga cccgcatcag cccggcctcg gcctggcgga aggcggtgac gtcggtgaag
    13441 gtggcgacga aaccgccgcc cggcatcggg ttgccgcgga tctcgatgat gctgccgtcc
    13501 gggaacacgc gttcggacag gtgggtggtg ccggcgcgca tgtgttccag ccggcgctgc
    13561 agggcatcgc cgtggcggtc gatgccggcc acctcgcgca gcgcccacgc cgaggcctcg
    13621 gcaatcggca ggccgacccg cagcatcccc ggcgggaagc cgaacagctc cgcgtagcgt
    13681 ccgttccagg ccaccaggcg caggtcggca tcgaccacgc tgatgccctg gctcatgttc
    13741 tcgagcgcag cctccagcac ccgctggttg aagcgcaggt cctcgctggc ctcgccgacg
    13801 atcgcggcga cggtgtcgag gtcggcgccg ctgcggtcaa ccgccgccgc atcgcgccgc
    13861 gcggcatcca gcagcaggcg tgccgaggcg ctgcccagga tcgcagccag ttcgtgttca
    13921 aggcgcgatt cgatcacggc tggcaccggc cccttcgccg gtgccgcgcg caacagttcg
    13981 tccacccgct gcgccggcag gaagcgccgg ccggcgttgc gcagggtgag caggtcggag
    14041 ccacgcgcct cgcgacggcg cggcgtgccg cgcaggctgg ccagcagcac ggttgtcgcg
    14101 ctgccaacga acaggctgac cccgaccgcg cggccaagcc ggctccagcc ggtcaacccg
    14161 aacagcgcct cgggtgccag ccagtgcatg ccgaacggac cctcgcgcag ccacgcgggg
    14221 tcggcaccga tggtcgccgc gatcaacggc accagcatca cccacgacca cgccgcgaac
    14281 cccgccagca cgccgaagct ggccgcgttc gcaggcgtct gcgggcgcca caccgcgaac
    14341 gccagcgccg gcgccagcgt cgccaacgcg gagaacgaga ccgcgccgac gtcagccagc
    14401 gcctcgttgc cgctgaccag ccggctgtag gcccaaccga gcagcatgat cgcgatgatg
    14461 ccggcgcggc gcagcagcag caggtcgccg cggtggtcac cggaaccgtc gtcaccgatg
    14521 ccgcgcgccc acgcgccgcg cagcaggccg ggcgcgaacc agtggttgcc gatcatcagg
    14581 ctgagggtca gggtgctgac gatcaccatg ccggtggcag cgctgaggcc gccgaggaac
    14641 accagcagcg caaccccctg gttgccctgc gccagcggca gcgccagcgc atacatgtcg
    14701 gatggcaccc gctcgccgaa caggctggca ccggcgcgcg ccagaggcaa cgccggcaac
    14761 gcgatcagca gcaggtacag cgggaattgc cagcgcgcgg tgcgcacgtc gcgatcgtcg
    14821 cggcattcca ccacgccgac atggaactgg tgcggcatca ggaacatcgc cagcgcgccg
    14881 aggatcacca gcggcacgaa gcccccggcc ggcgcggcag cctgcgcgtg cggcaccgcc
    14941 ggcagcgtgt ccagcccgaa ccacacgaac gcgcccagca ccagcatcgc cagcagcttg
    15001 aacagcgact cgaacgccat cgccagcacc agcccgcggt tgtgctccgc ggcactggcg
    15061 cgacgggttc cgaacaggat cgcgaacagc gccatcgcca gcgccacgta cagcgcgccg
    15121 tcgcgcaata ccggtggcgc gccctggccc gcggcgccgg caccactggt gagcgtcgag
    15181 aaactcatcg tgatcgcctg caactgcagg gcgatgtagg gaatcagccc gagcaccgcg
    15241 accagggtga ccgtggccgc gagccacgca tccttgccga gccgggtcgc gatcaggtcg
    15301 gccagcgagg tggcgttgct ctcgcgcgcc agccgcacca gccggatcat gaagaacacc
    15361 gcaagcgcgt agcacaggat cgcgccgatg aacgtcggcg gcagcggcca gccgtagcgc
    15421 gcggcctggg tgacggtgcc gtagaaggtc caggaggtgc agtgcaccgc cagcgacagc
    15481 gcgtacacat gccgccagtg cttcgcgaac acgcccgggt ggcgctccgc gtacaggccg
    15541 gtgccgaaca gcaggccgag ccacgccaac gccgcggcga ccaccgcggc gacgctcagc
    15601 atcgcgccac catcaggcat gtcgggaggc gttggtcatc gatccgcatc tgccggcgag
    15661 cataacgcga cggcgggccg aagcccgccg ccgcctggtc cgcgtggtac cgcgaagggt
    15721 cagaagttgt agcgcacgct caggctgtac tggcgcggcg gaccgtagaa cccggtgtac
    15781 acgcccaatg cacggtttag gttgtagccg gtggtcaggt actgcttgtc ggtcaggttg
    15841 gtaccctgca gcgacaccgt ccaggccggg ttgatgttcc agatcacgcc ggcattgagc
    15901 aggccataac cgtcctgcga gatcggcggg cgcccgttcg gcgggacttc cgcgcggttg
    15961 atctcggtgg tggcaaccac cgaagcctgg tagctgtagc cgatccgcgc cgaaatgtcg
    16021 ccggcgccga ccggggtccg gtattccacg ttcaaggcgc ccgagaaatc cggggcgttg
    16081 gtgaattcct gctcgtcggc gatgttgacg cccgcgaaca tgaactcctc gtactcggtg
    16141 tccagccagg ccaggttgcc gctgaccagc cagtgctggc tcggcagcca ctggtactcg
    16201 acttcgaagc cgttgaccgt gccggaaccg gcattggtga agtcgccgaa gaaggcgtct
    16261 tcggtgccgt cgccgttgct gtcgtaggcg gtgaacaccg acagctggat gtccttgtac
    16321 ttgttgtgga aggcggacag gttgaggaac agcgagccgt cgagcaggcc catcttggtg
    16381 ccggcctcgt agctgtccac ctgctcgtcg tcgaacggct ccgccgaacg gggcacggcc
    16441 gtggcctggg cgcggatgtt gtagccaccg gacttgaagc cgcggctggc aaggccatag
    16501 acgaggatgt ccggcgtgac ctggtagtcc agcgagatct tgggcgagaa gttgttgaag
    16561 ctgatggtct tgtcgaagtc ggcggccacc gagatcggcc ttgtgtaggt ggcgtccgag
    16621 tagccgcggt tcagcaccac cgcatgcttt tcctcgttgg tgtagcgcgc gccgacgtcg
    16681 accttgaact gctcggtgac gtcgaaggtc cagtcggcgt aggcggcgta gctttcggtg
    16741 tagacattgc cctgggtgtc gccgaacagg aagttgaaga agttgttgag cacctgcccg
    16801 ccggcgtagc cgtcgaacat gtacacgccc atcaccccgc gcgagcgccc gccgccgtcg
    16861 tacatcgcct gcagctcgtg ggtgacctgc tcgtcgtcgt agaaggcctt gacgtcggcg
    16921 atcttggact gggtcacgtc gaagtcgatg ttggtctcgg tatccgactc gcgcttggcc
    16981 agcacgtact tgaaggtcca gtcgtcgctg gcgcgcaggt tcgcggtgac cgaggcgccg
    17041 ctcatggtgg tctcgttgac gttcggcatg ccgttgcgga cgtcgtagcg gctctccagc
    17101 ggcggcgtgt tgggcacgtt gcggttggcc tgaagcatct gcgcgccgcg cacgccggag
    17161 ccgtccttca tgtggtcgag cgcgaactgc aggtcgaagt tgtcggtgac gtaggcgccg
    17221 aggctgaggc ggtacgcggt gacgtccttg tcgctgaccg ggtcgccggt gacgatgttc
    17281 tcgccgaagc cgtcgttgtg caggtcggcg atcgagatcc ggccgcgcag gtattcgctg
    17341 ccgccgaggc cgccacccac cgccgccttg atgtcgcgct ggccgtagtt gccgatggtg
    17401 agggaaccga agccgttgaa gtcggagcgc aggcccctgg agatgtactt gatcgcgccg
    17461 ccgatggtgt tcttgccgta cagcgtgccc tgcggaccgc gcagcacttc aacgcgctcg
    17521 acatcgaaca cgtccagcag tgcgccctgc ggccgcgcga tgtacacgtc gtccatgtac
    17581 aggccgacgc cggggtcgac gccccacagc ggatccgact ggccgacgcc gcggatgtag
    17641 gcggtcagcg tgctgttgga gccgcgcgcc gcatagatgg tcaggttcgg cacctgtgcg
    17701 tcgagatcgg acaggtcgcg gacgttgagc ttgtccaggg tgtccgcggt gaacgcggtc
    17761 accgcgaccg gcacttcctg cagcgtttcc tcgcgcttgc gcgcggtcac cttgatcgac
    17821 tcgagggtgg tggtcttctg cccgccagca tcggctgcgg cggcgtcctg ggccatcacg
    17881 ggcatcgttg ccagcagcga cacggccagc acgccatgga tcgccagact caattgcttg
    17941 cgcttcatct ttcccctcct gagggcacat cgaacgctgc ggacgggcgc cgcggttgag
    18001 cgcatggtgc gtggatgggc ggcaccgcgg cattggacat tcggacaatg ggccgcgccc
    18061 ccctcggcaa ccacactcgc atccctcagt cggttgcccg gagcggcaga tggcattcct
    18121 gatcgtgctc gccgcgttgt gcttcctgat gttcgtggcc taccgcggct acagcgtgat
    18181 cctgttcgcg ccggtggcgg cgctgggcgc ggtgctgctg accgacccgc agctggtggc
    18241 accgatgttc accggcctgt tcatggacaa gatggtcggc ttcctgaagc tgtacttccc
    18301 ggtgttcctg ctgggcgcgc tgttcggcaa ggtgatcgag ctgtccggtt tctcgaagtc
    18361 gatcgtggcc gccaccatcc gggtggtcgg cgcgcagcgg gcgatgctgt cgatcgtgct
    18421 ggtgtgcgcg ctgctgacct acggcggggt gtcgctgttc gtggtggtgt tcgcggtgta
    18481 tccgttcgcc gccgaactgt tccgccagag cgacatcccc aagcggctga tccccggcac
    18541 cattgccctt ggcgcgttca ccttcaccat ggatgcgctg ccgggcaccc cgcagatcca
    18601 gaacatcatc cccaccacct tcttcggcac cgacacctgg gccgcgccgg tgctgggcac
    18661 gctgggcggc atcttcatcc tgatcctggg catgctgtac ctcgaatcgc gccgtcgcgc
    18721 cgcactggag gcgggcgaag gctacggcga tgccgccagc ctgttcaacg agcccgtgcc
    18781 gttcgccggc ggcaagcttg ccaatcccgc cgttgcgatc ctgccgctgc tggtggtcgg
    18841 catcgtcaac aagctgctga cgctgtggat cccgcaacac tacggcgcca gccacagctt
    18901 cgacccggcg gtgatcggca gcgccgcgcc ggtggtgcag gaagtctcga aggtggcagc
    18961 gatctgggcg gtggaaggcg cactgctggc cggcatcgcc tgcgtgctgc tgttcgcgtg
    19021 gaagccggta acgaccagct tcgcggaagg cagcaggtcg gccatttccg gcgccttgct
    19081 ggccggcatg aacaccgcct cggaatacgg cttcggcgcg gtgatcgctg ccctgcccgg
    19141 gttcctggtg gtcgccaacg cactgggatc gatcccgaac ccgctggtca acgaagcggt
    19201 caccgtcact gcgctggccg gcatcaccgg ttccgcgtcc ggcggcatga gcatcgcgct
    19261 ggcggcgatg gcggagacct tcatcgccaa cgcgaacgcc gccgggatcc cgatggaagt
    19321 cctgcaccgg gtcgccgcga tggcctccgg cgggatggac acgctgccgc acaacggcgc
    19381 ggtgatcacc ctgcttgcgg tgaccggcct gacccatcgg caggcctaca aggacatttt
    19441 cgcgatcacc tgcatcaaga ccatggcggt gttcgtggtg atcggcctgt attacgcctt
    19501 cgggctgacc tgaagtcctt gccagcgcaa tgccatggcc cgcaccatgc gcggccatgg
    19561 atgcccgccg cccacccgcc ctttccgccc gcacgacgcc cacctgggag gtcgaactgc
    19621 tgatctccgg cgtggccgtg ttcgccatgc tgcagttgcc gggctggctc gacgaccgca
    19681 tgttcgcact ggagccgcgc gtgggcagcg actggcggcc ggtgctgctg ctgttctaca
    19741 tctactcgaa gagcacggcg gtggtgctgg ctgtcacctt cgcgatccac ctgctgctgc
    19801 gtgcgcaatg gatcgcactg gtgggcatgg actcggtgta tccacaaggc gtgcagctcg
    19861 accggctgcg gatgggaccg atccagcgcg ccatcgagga agcacggccg gaaagcagcg
    19921 ccgacgccat cgaacgcgcg gacaaccggg ccagcgtgct gttcgcgatc ggcgtcagca
    19981 tcgccctgat catcgcatcg atctgcatct tcttctgcgg ctcgctgctg ctggtgaacc
    20041 tgcttgcgca gtcgttcgac tggccgatcg acccgctgaa agtcatgctg ggcctcttcg
    20101 gcgcgctcat gctcccgttc atggccgcca gcatgatcga ccagttccgc ggcgaccgcc
    20161 tgcggcccgg cggggccacg catcgcgtca tcgccgcaac gctgcggttc tacacgcgga
    20221 tcggcatggg ccgccggaac agccacgtcg tggcgctgtt gaccagcaat ggcggcgaac
    20281 gccggatgat ggcggtggtg gtcggcatca tggttgccgc actcctgggc gtcggtgccg
    20341 cctacacggc gatgggcagt cccggcgcga tcggaagcta cgccctgttc cccgattccg
    20401 gacacctgca gctgcgttcc gaacactacg acgaccagcg caatcccgcc cgtgacgcgg
    20461 cccttccctt cgtgcagtcc gccgtgatca ccggccctta cgtgaagctg ctggtgccgt
    20521 accggcccga ccgcgaccag cccgcgatgc gcacgcactg cgcgcacgcc cgcctcctgc
    20581 agggcgatgc cctggcgacc gccaggctcg cctgcctgca ggcgctgcac ccggtctcgc
    20641 tcgacggcaa gccattgccg ggcctgcgct acgagatcgc cagcgacccg cggaccgacc
    20701 gccccgcgct gttggcgatg atcgacgtgc gaaacctcgc gccgggtcgc cacgacctgc
    20761 gcatcgcgcg cccgcctcgt ggcgaccgca gaccggacaa cgacaacccg gatcccggct
    20821 tcgacgccat tgcgttctgg cgctgatcct gcgacgcgca cggcctagaa tcgcggcgtg
    20881 agcgaagcgc cgcaacgcaa gatcatccac gtcgacatgg acgcgttcta cgcgtcggtc
    20941 gaacagcgcg acgatccatc gctgcgtgga aagcccgttg tggtggcctg gcgcggcgcg
    21001 cgctcggtgg tgtgcgccgc cagttacgaa gcgcgcgtgt tcggcgtgcg ctcggcgatg
    21061 ccggcggtgc gcgcggaacg cctgtgcccg caggcggtgt tcgtgccgcc ggatttcctg
    21121 cgttacaagg cggtctcaag gcaggtacgc ggcatcttcg agcgccatac caacctgatc
    21181 gaaccgctgt cgctggacga ggcctacctc gacgtcagcg cgccgaagca tgacctcggc
    21241 agcgccaccg cgatcgcgtc gtcgatccgt gacgcgatcc gcgaggagac ccggctcacc
    21301 gcatcggccg gcatcgcacc caacaagttc ctggccaaga tcgccagcga ctggaacaag
    21361 ccggacggcc agttcgtgct gaagccggcg atggtcgatg cgttcctggc gccgctcgcg
    21421 gtgggcaagc tgccgggcgt gggcaaggtg atggaggcga agctggccga gcttggcatc
    21481 gccacctgcg ccgacctgcg cgcattcggc gatgacgcgc tggaacgccg gttcggccgc
    21541 tggggccggc gcctgcacga actgtcgctg ggcatcgacg aacgcgcggt gcaacccggg
    21601 cggcccaccc tgcaggtgtc cgcggaggac accttcgagc aggacctccc gctgacggca
    21661 cttgaactgc atatccgccg ccttgcggcc aaggcctggg ccggttacct gcgcgaacgg
    21721 caacggcacc cggagcgcat cgcgcgcacg gtggtgctca agctgaagac cgcggatttc
    21781 cgcatcctca cccgcagcct gaccgccaac gcaccgcccg cctgcgaaac cgcgttcgcc
    21841 gatgccgcct gcggcctgcg cgagcgggtc gggctgcccg ccggcacccg cttccggctc
    21901 gccggcgtgg gcctggccgg cttcgtcgat gccgacctgg cgcgggcgca ggacgacctg
    21961 ttcgccggcg cccggggcag tcgcgacggt tgatcgccgc acccacggcg cgcatctgaa
    22021 cattcgctcc aatcgcgcgc gctacggtgc aaggatgcag gccggcaccc caatcgacgg
    22081 cgctccagcg gacgccacca cgccccccgc gatggcggac gacctgcgtg gcgtcgcgcg
    22141 actgctggtg cggggcgtga ccgccaccac cgacctcgcc gagggcgtgc acgcgggcat
    22201 cctcgggcgt cccgggcctg ccggcggatc cggcgccagc acccgcggga tcccgcggat
    22261 cgcctaccag ggcgtgcgga tgaccgccaa cctggtcggt ggcgcgctgg atgcgtcgct
    22321 ggcccggctc accccgtggc ttggccccag cgccggctca agccgccgcg atgcgctggt
    22381 cgctgtgctg aatggcgtac tcggcgacca tctggccgcg agcgcgaacc cattggcgct
    22441 ggaagcgcgg ctgcgggtcg gcggccgcgc cctgcccctg cgcggcgaag actggccatc
    22501 gctgccgttg gcgccgacgc cgcggctgct ggtccaggtg cacggcctgt gcatgaacca
    22561 cctgcagtgg cgccgccagg gccatgacca tggcgaacgg ctggcggccg agctgggcta
    22621 caccccggtc cacctgcact acaacagcgg cctgtcgatc gagagcaacg ggcgcacgtt
    22681 cgcaaccctg ctgcagcagc tcatccagga ctggccggtg ccggtcgagc gcttcgcgat
    22741 cctcggccac agcatgggtg gcctggtggc gcgcgcggcg atcgcatccg ccctgcagga
    22801 gcgccagcaa tggctcgagc ggctggacca gctcgtgttc ctcggcaccc cccaccatgg
    22861 cgcgccactg gagcgtgccg gccacgtgct gcagtcggtg ctgggcctga ccccgtggac
    22921 ggcgccgttc atgcgcctgg gcgaggtccg cagcgccggc atccaggacc tgcggcatgg
    22981 cctgggcgaa gacctgccgc tgccgccggg cgtgcgtgcc tatgccgtcg ccgcctcgct
    23041 gcagggcgtc cgcgccggac acgaccaccg gcgcctgcgc ggcgacgggc tggtgccggt
    23101 gcgcagcgcc ttcggtgatc accgcgaccc gctgcgggcg ctgggcatcc cggaggcgca
    23161 tcgcctgctg gtggagcgca ccggccacct cgacctgctg aacagccacg ccgtgtacgc
    23221 cacgctgcgg cactggctgg cctgagcttc caccggggcc ggccgatgcc ctaggcttgc
    23281 gcggatgacc ccgcatgcca cccctgcgct ctcccgcgca caactcaaga ccctggcgct
    23341 ggcctcgctg ggtgccgcgc tcgagttcta cgacttcgtc atcttcgtgt tcttcgcggc
    23401 cacgatgggc gcgctgttct tccccgccga catgccggaa tggctgcggc tggtgcagac
    23461 cttcggcatc ttcgccgccg gctacctggc acggccgctc ggcgggatcg tgatggcgca
    23521 tttcggcgac ctgtccgggc gcaagcggat gttcatgctg agcatcctga tgatggcgat
    23581 cccgaccctg ctgatgggcc tgctgccgac ctacgcacag gccggcgtgc tcgcaccgat
    23641 cctgctgctg ttgctgcgga tcatgcaggg cgcggcgatc ggcggcgaag caccaggcgc
    23701 ctgggtgttc gtcagcgaac atgtcccgcc gcgccaccgc accctggcct gcggggcact
    23761 gtcggccggc ctgtgctccg ggatcctgat cggttcgctg gtcgcccgcg cgaccgatgc
    23821 gatgttcgac caggccgaag tgctggcctg gggctggcgc ctgccgttca tcgtcggcgg
    23881 ggtgttcggc ctgctggcga tggtgctgcg gcggcagctg cacgagaccc cggtgttcac
    23941 cgagctgcgc gcgcgtcgcg ccctggccgc ggagctgccg ctcaagaccg tggtccgcga
    24001 ccatgccggc gcggtgttgc tggcgatgtt gctgacctgg ctgctgaccg cggcggtggt
    24061 ggtcaccctg ctgatgatgc cgaccctgct gcagggcctc ggggtcagcc gcgcggatgc
    24121 gctggccggc aacaccctcg ccatcttcgc caccgtactc gccaacctgg ccgccggctg
    24181 gctggccgac cgcttcggcc ccgggcgggt gctggtgctg tggagcgcgt cgctgggcgc
    24241 ggcgttctgg ctgttctacg gcaatgcgct ggcgggcgcc tattcaccgg cactgtatgt
    24301 cgtggccggt gcctcggtcg gcctgaccgc tctggtgccg gcgctcgcgg tgggcgggtt
    24361 cccgccgcaa gtgcgctttt ccggcctgtc gttcgcctac aacctggcct atgcgatcgc
    24421 cggcgggctc accccggtgc tgttgagcct ggcgatccgc gacaacccgg ccgcaccgat
    24481 gcagtacatc gcggcgatgg cggtgctcgg gatcgcgctc gggctgtacc tgtcggtccg
    24541 gccgccgcgc catggcgcgc gctgagcggg tgcgggtgga aatgcacagc cagcggccag
    24601 gccccgatcc ggatccacgc ccgcaaccgc cgcagcggcc gttgccgact gactgctgcg
    24661 acagcggctg cccggtctgc gtccatgacc tgtatgccga cgaggtcgcc gcctacgcga
    24721 aagcactggc ggactggaac cagcggcacc cggatccggg cgggtcgcca ccagccgctt
    24781 agtcgaacgc cggacggacg cagccgccac ccgataaacg aaaagcgggc cgaagcccgc
    24841 tttcgttgac agcctgaggc ctgggccggc ggattactcg gccgtgccct cgccttcggc
    24901 cactgccttc atcgacaggc ggatgcggcc ctgcttgtcg acttccagca ccttgacctt
    24961 gacgatgtcg ccttccttga gcacgtcgga caccttttcg acgcggtcgt tggagatctg
    25021 cgagacgtgc accaggccgt ccttgccggg ggaaatggtg acgaacgcac cgaagtccat
    25081 cagcttggcg accttgcctt cgtagatccg gcccggctcg acgtcggagg tgatctgctc
    25141 gatgcgcgcc ttggcggcct ggccggcggc ggcgttcacc gaggcgatgg tgatggtgcc
    25201 gtcgtcctgg atgtcgatct gggtgccggt ttccttggtg atcgcctgga tcaccgaacc
    25261 gcccttgccg atcacttcgc ggatcttgtc cgggtggatc ttgatggtga tcaggcgcgg
    25321 cgcgaagtcc gacagttccg cgcgcggagc ggtcagcgca ctggccatct cgccgaggat
    25381 gtgcaggcgg ccggccttgg cctgcgccaa cgcgaccttc atgatttcct cggtgatgcc
    25441 ctggatcttg atgtccatct gcagggccga gatgccgttg ctggtgccgg cgaccttgaa
    25501 gtccatgtcg ccgaggtggt cctcgtcgcc aaggatgtcg gacagcacca cgaagtcgtc
    25561 gccttccttg accaggccca tcgcgatgcc ggcgaccggc gccttgaccg gcacgcccgc
    25621 gtccatcagc gccagcgagg agccgcagac cgaggccatc gacgaggagc cgttggattc
    25681 ggtgatctcc gaaaccacgc ggatggtgt
//
LOCUS       DAORUK010000009        25508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_1495_length_25508_cov_19.138451, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000009 DAORUK010000000
VERSION     DAORUK010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 25508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25508
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1495_length_25508_cov_19.138451"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..83)
                     /locus_tag="PK818_01270"
     CDS             complement(<1..83)
                     /locus_tag="PK818_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HQY81396.1"
                     /translation="MAIKVGINGFGRIGRCVFRAAVQNFGD"
     gene            171..1040
                     /locus_tag="PK818_01275"
     CDS             171..1040
                     /locus_tag="PK818_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006452857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HQY81397.1"
                     /translation="MRLARPRKFAVLLATALATVSVAGPAAAWGALGHRLVGELAERH
                     LDPRARAEVAQLLQGEADPSLAGVSNWADELRTQDPERFKATSRWHYINAEGGGCAFD
                     VARDCPADDCVVAALEAQRLILADRSQPLAARRDALKFIVHFVGDVHQPMHAGDRDDS
                     GGNRFQVSLRTPLEPEAYARKNYVDGVMGTNLHSVWDFYVLAGLGLGFRDYAARLDAL
                     PWPRYPQPSPNDFLPKAWAGESCRLIEARALYPAQPAIDHGYLDSMRPLAEQRLRQAA
                     WRLAQLLDQTLAR"
     gene            complement(1042..1398)
                     /locus_tag="PK818_01280"
     CDS             complement(1042..1398)
                     /locus_tag="PK818_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HQY81398.1"
                     /translation="MSQSFPDLIDDLNAAIGTLRAGAPEPMRAFSGLAREALKPGALD
                     VRTKELIAIGIAVATRCDGCVGFHAKAAIKAGATREEVLETLSMAIYMGAGPSMIYAA
                     EALRAFDELAAPPPAA"
     gene            complement(1415..1849)
                     /gene="trxC"
                     /locus_tag="PK818_01285"
     CDS             complement(1415..1849)
                     /gene="trxC"
                     /locus_tag="PK818_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010365869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HQY81399.1"
                     /translation="MNEAMIVACPLCNALNRVPQARLGEAPNCGQCHRALFAAAPLAL
                     DEAGFHAHVERAGLPVLVDFWAPWCGPCRAMAPQFEAAAAQLEPRVRLAKVDTQAQPT
                     LAQKFAIRSIPTLVLFQHGREIARQPGAMGAADIVRWTRQHL"
     gene            complement(1863..2504)
                     /locus_tag="PK818_01290"
     CDS             complement(1863..2504)
                     /locus_tag="PK818_01290"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HQY81400.1"
                     /translation="MIRTTLCLPPLLAVLLAACSSAPPPQPAAAVAVPAAPAPLEAPA
                     PTAPQVRAQAAAKAFSSSLKQALGAELAKGGTTGAITFCHAQAPKIAAQVAAEHGVRI
                     GRVPVPGRQRSPANAPDAWQADALRALIARHDAGTPVAELVQVVDSGLPDGVALRMMR
                     GIPVEPACLACHGKALADDTRAALERLYPGDTATGFDAGDLRGALWVEVPALD"
     gene            complement(2501..5740)
                     /locus_tag="PK818_01295"
     CDS             complement(2501..5740)
                     /locus_tag="PK818_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY81401.1"
                     /translation="MTEPRRFGLSGRLAAAFQANPLTPILALLGLLLGLVAVLVTPRE
                     EEPQIDVTMANVFVPFAGASARDVEQLVSTPLEQKLSEIEGVKHVYSVSRPGMAVLTV
                     EYTVGVERQPAIVRLYNQVYSNQDWLPQGVGVGQPLVKPKGIDDVPVMALTLWSDDPA
                     RGATALAEVAHTLETELKRIPGTRDVYTIGAPDRVVAVTLDPARLASHGLTVADLGQA
                     LRAANAVQQVGERVGGGVATPVTAGTFLASAEDVRDLVIGLDGGRPLRLSDVAQVHAG
                     GDIASRYAWHGAPAGKAGPAQGAAPAVTIAIAKKPGSNAADITSAVARRIGQLKGELI
                     PEGVEVTVTRDYGRTAADKAAKLMQKLAFATASVVLLVLFALGWREAIVVGSAVVLTL
                     ALTLFASWAMGFTLNRVSLFALIFSIGILVDDAIVVVENIHRHMARGGKSLREAIPPA
                     VDEVGGPTILATFTVIAALMPMAFVSGLMGPYMRPIPINASVGMLLSLAIALIVTPWL
                     SLKLLARHGHAGEADHAPDGEPQGRLHRLFRRVMSPFLLGAGAGRKRGLLFASMVALV
                     ALAASLAVFQLVVLKMLPFDNKSELQVVVDLPEGRTLEDTNALLVELAAVVERMPEVL
                     DYQGYAGTAAPINFNGLVRQYFLRSGSNVGDLQVNLVDKHHRDRQSHAIARELRPRLA
                     EIGRRHDASVKVVEVPPGPPVLSPLVAEVYGPDYAASRKLGKALEARFLATEGIVDVD
                     TSVESDAPREVIVVDRARATRLGVPQAAISDALAAAVAGLDATYVHDGASKYPRPVRL
                     RLPPAEQAAVDRLLALKVRGGQGQLVPLSELVAVRRAPWDGAIHHKDLLPVVYVMGDE
                     AGELDSPLYGMFDLVGQVSADAIGGHQVAQSFIAQPADTSGFAIKWDGEWQITYETFR
                     DMGIAYAAGMVLIYLLVVAQFRSYLVPLVIMAPIPLTVIGVMPGHALLGAQFTATSMI
                     GMIALAGIIVRNSILLVDFINQEVDRGVPLVDAVIDACAVRAKPIVLTGVAAMMGAFF
                     ILDDPIFNGLAVSLVFGILVSTVLTLVVIPLLYYVLLRRTGRRVQEVHA"
     gene            complement(5733..6815)
                     /locus_tag="PK818_01300"
     CDS             complement(5733..6815)
                     /locus_tag="PK818_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY81402.1"
                     /translation="MIDLRKTLLATGFLALAACGGEPAGTTTPALPALDTIEVTAASN
                     GAGSRWDGVVEAVRQAELTAQTAGRVTAVLVDVNTRVAQGQVLLRLSAVEQQAGANTA
                     RAQVRAAEAAAAEAEANYRRFSALAGGQFVSRAQVDQARAARDSAVAARDAARAQLAQ
                     AAQQADYTVVRAPYAGIVSARLVEPGESVAPGQPLLAVYAPGALRIQVQVPQSQADAI
                     RARPQARIELAGGEAFDAAQVVVFPAADPVTHSVGVRVLLADMPGAPAPGMTAKVVFP
                     IATNAAPSAVSLPASALMQRGEVSGVYVIAGNQVALRQLRLGQRSGDRVEVLAGLKPG
                     ERVAADPVAAVQALVAQRKAMERGDD"
     gene            complement(6895..7110)
                     /locus_tag="PK818_01305"
     CDS             complement(6895..7110)
                     /locus_tag="PK818_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HQY81403.1"
                     /translation="MTLDRAVQAFAGVMVVVSVLLTQFVHPNFFWLTAFIGFNLFQSA
                     FTGFCPAGMVMRKLGIGRNANDASCCR"
     gene            complement(7136..8425)
                     /locus_tag="PK818_01310"
     CDS             complement(7136..8425)
                     /locus_tag="PK818_01310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HQY81404.1"
                     /translation="MARIVVMGAGLGGMSAAYELREVLGKGHEVTLVGKGGQFGFTPS
                     NPWLAVGWRQQEQITLDVAGHVGKHGIAFNGDGVERIDAEGDAVITGGGERIPYDYLL
                     VCTGPKLAFEEVPGTGPDGGFTQSVCTTPHAAHAWEAYQAFLQDPGPVVIGAVAGASC
                     FGPAYEFAMIVDADLRKRKLRDKVPMTFVTSEPYIGHMGLGGVGDSKGLMESEMRQRH
                     IKWIVNSKVAEVRDGEMTVVEHDAKGQPGESHVLPFKFSMLLPAFKGVEPVAAVEGLC
                     NPRGFVIVDKHQRSPKYPKIFSAGVCVAIPPVEATPVPTGAPKTGFMIESMVTTIVRN
                     IQAELKGEAPDFEGTWNAVCLADMGDTGAAFVALPQIPPRNVTWTKIGKWVHLAKIAF
                     EKYFLYKMRNGTSEPIYEKYVLSALGIERLKDVKRSS"
     gene            8508..8900
                     /locus_tag="PK818_01315"
     CDS             8508..8900
                     /locus_tag="PK818_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQY81405.1"
                     /translation="MTKFLTAADLVAEAQARIREVPPQRFEGGFGDAVLIDVREPAEF
                     ETGHIPGAINIPRGVLEFQVDAHPAVANVSDPALSHRERPVVLACRTGGRAALAADNL
                     QRLGFADVRSIAGGVLGWAEAGLPLVVR"
     gene            8905..9255
                     /locus_tag="PK818_01320"
     CDS             8905..9255
                     /locus_tag="PK818_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQY81406.1"
                     /translation="MTQARRKPAPPVLDPESMRAHAADASRLLKALANDKRLMLLCLL
                     SEGERSVGELNARVDLSQSALSQHLAVLREDGLVTTRRDAQAILYSVAPGPAQRIIAT
                     LHDIYCGNDPPFCE"
     gene            9261..10139
                     /locus_tag="PK818_01325"
     CDS             9261..10139
                     /locus_tag="PK818_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008207510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY81407.1"
                     /translation="MGAPLQIEPFLHAGSGTWTHVVHRGDDAVVIDPVLDYDAASGRI
                     GVAGLQAVRDYLDTHGLQLRRILETHAHADHLSAAAVLRAATGAPVGIGDGIRQVQAH
                     FAQVFAIDGDDPALRDAFDATFRDGDAIEAGSLRFHVLALPGHTSDSLGYAIDGHVFV
                     GDSVFAPDIGTARCDFPGGNMQALHASIQRLHAMDEATVLHLCHDYPPAGRGPRSSVT
                     VGESRRDNRMLNAGTTLEAFVDARRARDAQLAAPALLYPALQVNIRGGRLPPAQANGR
                     HYLATPLQGELPAGLA"
     gene            10210..10959
                     /locus_tag="PK818_01330"
     CDS             10210..10959
                     /locus_tag="PK818_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQY81408.1"
                     /translation="MTSPDLLLAALFFAVAVLYSSVGHAGASGYIASMALLGFAPEQV
                     RPTALALNLLVGGIGLYRWWRGGHVRWRNVLPFVLASAPAAFFSAQIKLPKESYSLLL
                     GIVLLVAAIGVFRHASRAESEDAAAQGRNVPWLAGLLVGAAIGTLSGLTGTGGAIFLT
                     PLLLFAHWMPTREASGTSVAFVWINSLTGLAGLLHSGQSLPAMLPWWLGVVAIGALIG
                     TQMGLKWLPVRGLRYALCAVLLIASGKLLLA"
     gene            complement(11020..13005)
                     /gene="tkt"
                     /locus_tag="PK818_01335"
     CDS             complement(11020..13005)
                     /gene="tkt"
                     /locus_tag="PK818_01335"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011348449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQY81409.1"
                     /translation="MTSPSRRDLANAIRFLAIDAVQAANSGHPGMPMGMADIAEVLWN
                     DYLKHNPANPAWPDRDRFILSNGHGSMLQYALLHLAGYDLPLDELKAFRKLGSKTPGH
                     PENTHTAGIETTTGPLGQGLANAVGFALAEKLLAQRFNRDGHAIVDHRTWVFLGDGCL
                     MEGISHEAASLAGTWGLGKLVAFWDDNHISIDGNTDGWFTDDTPARFEAYGWNVVRGV
                     DGHDPVEIRTAIETALESSGKPTLICCRTTIGFGSPNKAGKESSHGSPLGKDEVQATR
                     DALGWTHGAFEIPQPLYAGWRARDLAAAESAWNARFAAYKAAHGDAAAEFERRMAAEL
                     PGGFGADADAFIARLQQDGPVIASRKASQNAIEAFAPLLPELVGGSADLAHSNLTLWK
                     GSKSVATDDPNANYIYSGVREFGMTAISNGLALHGGFIPYDATFLVFSDYARNAVRMS
                     ALMRVRAIHVYTHDSIGLGEDGPTHQPIEHLASLRYIPDNDVWRPCDAVESAVAWKAA
                     IERADGPSCLVFSRQNLPHQPRSDAQLAAIARGGYVLKDSVGAPEVILVATGSEVGLA
                     MQAAAQLGDGVRVVSMPSTDVFDRQDAAYREAVLPKACRKRVAIEAGVSDFWRKYVGL
                     DGAVVGIDSFGASAPIEALLPHFGFTVDRVVAAAQAL"
     gene            complement(13096..14430)
                     /locus_tag="PK818_01340"
     CDS             complement(13096..14430)
                     /locus_tag="PK818_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HQY81410.1"
                     /translation="MHSAASASPVASRGWPLHVKVLLGFVLGTLAGLAVHALAADAAW
                     VQGVIEYATKPFGQVFLNLLFMLVVPLMFSALVLGVAELGDIASLGRLGWKTLTYTAV
                     VTALAVGIGLACANLFQPGTHMDPALVEQAMSNARTGEIVSKGSELRLMDLLVNIVPH
                     NIVGAMGDDKQKLGIVFFALMVGIGLVMKPTPGTRAFKSALHGLFEICMHLIGMFIKL
                     APYAVAAFMFNLTSQLGWDVIRSLLWFVLTVLLALGLHGLVVLPLWVRFMGGMAPRTF
                     FRGTQEAVLTAFATASSTATLPVTLRVAEENLKLPRKVSRFVLTVGASANHHGTALFE
                     GITVLFLAQVYGLHLGLPQQLMVLALCILGGIGTAGIPSGSLPVIAMICGIIGIKPEG
                     IGIILGVNTFLDMCRTSLNVTGDLATAVVVAHRSGEKDLPDEALAQLDDIVR"
     gene            complement(14483..16141)
                     /locus_tag="PK818_01345"
     CDS             complement(14483..16141)
                     /locus_tag="PK818_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HQY81411.1"
                     /translation="MNRARAFGLLVALCALPGLVTGVSAQTRAWLDRAQVTYGETVTL
                     NIETSASVRQVDYRPLAAQFDIAGQTVRRSFGMVNGQSRAKSLFAVGIRPRGPGVITV
                     PALRVGNASTSPLRLTVLPPTVQQAGGDAEAFVETEFDATQPYVQQAVGMAVRLHVAV
                     PVLSGQLDQDQVEGASLEQVGADIQYQREIGGRRYSVVERRYLLVPERSGPLRVPGAR
                     FNGQAVGGFFDNIFDDGRKPLAAAAPDQRLQVRPVPADAPQPWLPLRDLRLRYLQSPR
                     DARVGEATMVELEAIADGARASQMPVPGLAETDQAQVFADPVQADEQFIEGRPRTTLR
                     RRIAIVPLKPGALSLPGPRIEWWDAGQGVARTAMLPPLQLQVSPALDAPVGSGEVPRA
                     PATAGETPDPRASAVAPVRDRRGWLLLAAVALAVIAAFTWWWSRARRASVRMAEVPGA
                     SPQPAAARSPSLAEALRQGELSAIARALSGEAGVRGDDLDGVRARLDDTAQREAIERL
                     QAARWGDGDAAVALVALRGAFAQGPRWRMRGRAPSSLLPPLYPR"
     gene            complement(16138..17844)
                     /locus_tag="PK818_01350"
     CDS             complement(16138..17844)
                     /locus_tag="PK818_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQY81412.1"
                     /translation="MSGLLAALPVHFLRPYWLLCLLALPLLAWWWRARAQPRSAWQDA
                     VDPHLLPHLLVAAEGSPRARLARGSGMLAAALAVIAMAGPSVGKAEHPLWQAKAPLVV
                     ALDLSSSILARDLPPSRLAQARAKIATLLRERAGGQVALVAFADDAYTVAPLTEDAAN
                     VALFLDALAPDVMPADGHRGDRAIAWSQRLLRNAGFAQGDVLLLTDRADGQAIAEAAR
                     ARAAGYRVSALGLGTAGGGAFDTPGGLGNARLDAASLQRLAASGGGRYQPLAAGNHDL
                     RALDVLDPQDAGAAAAGGGTSRRWRDDGAWLLPFALLFALPLFRRGGAAMAMLLLVLW
                     LPMPPAQAQDASGLWRRADQQAHARMLEGVAAYRRQQYEEAIAKFTGNASAEGQYNLG
                     NALAQAGRYDQAIAAYDRALQQRPGMPDAVANRAAVDAARKRKPPPGGQSQQGPQQGG
                     DQQRDQGQQRDAQQSRQPGRPQGDPSRQPGQGSQDKAADPRQQQQADAAQRQRMQDAL
                     RKQGQSQVPGQPRPQPAETPEQRERRIANQAQLQRVPDDPGALLRAKFQLEHQRRQGL
                     GQ"
     gene            complement(17841..18842)
                     /locus_tag="PK818_01355"
     CDS             complement(17841..18842)
                     /locus_tag="PK818_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQY81413.1"
                     /translation="MNALRELLGLDGFAWPWMLLALPLPWLVHLLVPARRSAQPALRV
                     PWNDRLLRIASGGSLAPTPRGFAWLAFLAWSLLCVAAARPQQLGPAIAPPQVGRDLML
                     AVDLSASMGEQDMELGGRIVDRLTAAKAVLADFLERRAGDRVGLVVFGERAFALTPLT
                     LDRASVQEQLGDSVVGLAGRATAIGDAIALATKRLQQQPDGQRVLVLLTDGVNTAGTL
                     DPAKAAQIARDNGVRIHAVAFGGDGGALSVFGFQLPMGGDEVDEAGLQRIAAVTGGRF
                     FRARDTDALAGIYAEIDALEPVQRQGQAVRPKIERYPLPLAWALGIGLLALVVGRRR"
     gene            complement(18839..19285)
                     /locus_tag="PK818_01360"
     CDS             complement(18839..19285)
                     /locus_tag="PK818_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HQY81414.1"
                     /translation="MTPLPLRDVHEGVAPAWWPPAPGWWLLLGAIVIVATLLGWRAAA
                     KRRRREAILRLFDDALAHAGTPAQQVAAMSELLRRAARRKHAGADRLLGDEWLRFLDE
                     GMPRPVFSTGAGRLLGDGGFRPQVAAQECAVLRDLARERYLRWMRA"
     gene            complement(19282..20085)
                     /locus_tag="PK818_01365"
     CDS             complement(19282..20085)
                     /locus_tag="PK818_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQY81415.1"
                     /translation="MQGHALSNLRGRGMEYAESREYAQGDDARHIDWRLTARSGRTHT
                     KLFQAERERLTLVVADTAPALYFGTRTRFKSAQAARAGALAAWIAVRDGDRIAALRSG
                     PGEAPVTPASGPRGALRVLDALVRWYARPPAANDGLAVALDHARRLLRPGSRLLVLAD
                     AASIVQVPLARWTALALHHEVIVLLLTDPLERTPPAARLAFASAAGDRVELGLDAAIT
                     RQRWRHEFGIPLEAALESLAACGIRAMELSSADPSDSWLPLLDRKPRPR"
     gene            complement(20214..21206)
                     /locus_tag="PK818_01370"
     CDS             complement(20214..21206)
                     /locus_tag="PK818_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQY81416.1"
                     /translation="MDTPTADTPRLHAAFSALREDLAREIVGQTGLIERLLIALLADG
                     HLLVEGAPGLAKTSAIRALAARLDAGFARVQFTPDLLPADLTGTEVWRPQDGRFEFLP
                     GPIFHPILLADEINRAPAKVQSALLEAMGERQVTVGRQTYPLPALFLVMATQNPIEQE
                     GTFPLPEAQLDRFLMYVRIGYPDGDAETEILRLARERARSAMQAGAPAPARIPIEDVF
                     AARRAVLDLHVAPALERYLVELVLASRDAARYDAALARRIAWGASPRGSIALERCARA
                     RAWLAGRDFVTPEDVRAVAPDVLRHRVLPSYEATAEGWDGERLVAELLQRVPLP"
     gene            complement(21350..23365)
                     /locus_tag="PK818_01375"
     CDS             complement(21350..23365)
                     /locus_tag="PK818_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ family protein"
                     /protein_id="HQY81417.1"
                     /translation="MTVTNVKQQRPLRRPSSLGACAVGLAVGLYAAGAGALPVVATAT
                     MAQSATGAMADPARRTPGAVSVTAIDFKRGDGGSGKLILRFSGDGAAPDLRNMGSSVV
                     VDIGNAQLPASLQRPINVTDFATPVQRIDARATSSGTQLVLGTQGTFESMAYQSGNEY
                     IVEVVPRATPVATAKTASPAMGATAMGATDSDSSIRYSGRPVTFNFQDVPVRTVLQLI
                     AEESNLNIVAADTVQGNVTLRLVNVPWDQALDIVLQAKSLDKRRSGSVVWVAPQKEIA
                     QFEQDKADARISLEERAEKVTEYMPINYGNAEDIAKLLTEGSKGNSSGQGGSGGQSAQ
                     SSQDRGFLSSRGSISFDKRTNTLLVIDIPQKVAEIRSLVQKLDKPVDQVVIEARIVVA
                     TESVARELGARFGISGRNENGYFSGDLETNTANVNADYQASQTNRALENSYNTCLGGA
                     NPSGCVPPTYVGSTTTRGLITNLAAGIFGSTNAPGSLALSILNAGYLLDVELSAIQEQ
                     GRGEVISNPRIVTSNQKESVIRQGQEIGYLTVTGGQSNNVPTVEFKEVLLELKVTPTI
                     TNDGRVFMNLAVKKDELEGFVSVGTFGDVPQIAKREVTTAVLVDDGQTVVIGGVYEFR
                     DRSDIAKVPFLGDLPFLGNLFRNKGRTKEKGELLVFVTPRVMRVAQR"
     gene            complement(23381..23851)
                     /locus_tag="PK818_01380"
     CDS             complement(23381..23851)
                     /locus_tag="PK818_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HQY81418.1"
                     /translation="MAVALAGCGSGRGDLEKWVAEVRAKPAPPLEPLPVMQQFETFEY
                     AAQNLRDPFSEAFTGVNGSGPRPDPGRRKQTLEEFPLDSLDMVGTLGAGSATAALVLA
                     PDKVTYRVRPGAYLGQSDGRVTAVYEDRIELVELVPDGAGGWLERPATMALEDN"
     gene            complement(23901..24593)
                     /gene="pilO"
                     /locus_tag="PK818_01385"
     CDS             complement(23901..24593)
                     /gene="pilO"
                     /locus_tag="PK818_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HQY81419.1"
                     /translation="MSTKKLSLRELDINNIGAWPRNAKIGFCVILALLIVGLAWWLFV
                     RDRRTELEGLQKQEVELRTEFETKQGRAANLEPLKQQLAQMEQQLQQMLRQLPSKTEM
                     PDLIVDISQTALATGIQNELFQPGPETPKEFYAEKPIALRMVGTYHQFGAFVSGVASL
                     PRVVIMTMHDISLKPKDAKTDPNARIGPNSALELSGTVKTYRYLDEDETAAQAAAATP
                     PAAGTPPATGGA"
     gene            complement(24590..25237)
                     /locus_tag="PK818_01390"
     CDS             complement(24590..25237)
                     /locus_tag="PK818_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HQY81420.1"
                     /translation="MARINLLPWRAERRKARQKEFVGMLGLAALCGVLVSFLIVSWYS
                     GRISSQTGRNEYLRGEITKVDGQIKEIEELDKKKGKLLARKEVIEQLQANRSQMVHLF
                     DSLVRTIPDGVTLTSIKQEGEILTLGGRSQSNARVSTYMRNLEGSGWMTGPDLNIIEA
                     KAGNAGLPYEFNLKVKLANPNAPKDEDGDGKPDAAPAATAPADVAAAPVAGGTAP"
     gene            complement(25237..>25508)
                     /gene="pilM"
                     /locus_tag="PK818_01395"
     CDS             complement(25237..>25508)
                     /gene="pilM"
                     /locus_tag="PK818_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HQY81421.1"
                     /translation="EAMVQQVSRLLQFFYAGSEFNRVDHVVLAGGCASIPGIAAMVEE
                     QLGVATTVANPLAQMTLGPRVQAHALAQDAPALLIACGLALRSFD"
BASE COUNT         3730 a         9174 c         8740 g         3864 t
ORIGIN      
        1 tcgtcgccga agttctgcac cgccgcgcgg aacacgcagc gcccgatccg gccgaagccg
       61 ttgatgccca ccttgatcgc catcgaaacc actcctgcag gggatgcgcc ggacatggcg
      121 cggtaatccg attattttag cgtctcccca ccgccccggc atcccgaccc atgcgactcg
      181 cccgccccag gaaattcgcc gtgctgctgg ccaccgccct cgcgaccgtt tcggtggccg
      241 gccccgccgc ggcctggggc gcgctgggac accgcctggt cggcgaactg gccgaacgcc
      301 acctcgaccc ccgggcccgg gccgaagtgg cgcagctgct gcagggcgag gccgatccca
      361 gcctggccgg ggtgtcgaac tgggcagacg agctgcgcac ccaggatccc gaacgcttca
      421 aggcgacctc acgctggcac tacatcaacg ccgagggtgg cggctgcgcc ttcgacgtcg
      481 cgcgcgactg cccggccgac gattgcgtgg tcgccgcgct cgaggcccag cgcctgatcc
      541 tggccgaccg cagccagccg ctggcggcgc gccgcgacgc gctcaagttc atcgtccact
      601 tcgtcggcga cgtgcaccag ccgatgcacg cgggcgatcg cgacgacagc ggcggcaacc
      661 gcttccaggt cagcctgcgc acgccgctgg agccggaagc gtacgcccgc aagaattacg
      721 tggacggggt gatgggcacc aacctgcatt cggtctggga cttctacgtg ctggccggcc
      781 tcgggctcgg tttccgcgac tatgccgcgc gcctggatgc gctgccgtgg ccgcggtacc
      841 cgcagccctc cccgaacgat ttcctgccga aggcgtgggc cggcgaatcc tgccgcctga
      901 tcgaggcgcg cgccctgtac ccggcgcagc ccgcgatcga ccatggctac ctggacagca
      961 tgcgtccgct ggccgaacag cgcctgcgcc aggccgcatg gcggctggcg cagctgctgg
     1021 accagaccct ggcccgctga atcaggcggc cggcggcggg gcggcgagct cgtcgaacgc
     1081 gcgcaacgcc tcggccgcgt agatcatcga cggcccggcg cccatgtaga tcgccatcga
     1141 cagcgtttcg aggacttcct cgcgggtcgc gccggccttg atcgcggcct tggcgtggaa
     1201 gcccacgcag ccgtcgcagc gggtggcgac ggcgatcccg atcgcgatca gctccttggt
     1261 cctgacgtcc agtgcgcccg gcttgagcgc ctcgcgcgcc agcccggaga aggcgcgcat
     1321 cggttccggc gcgcccgcgc gcagggtgcc gatggcggca ttgaggtcgt cgatcaggtc
     1381 ggggaaggat tggctcatgg gaaaggtccg tgggtcagag gtgttgtcgg gtccagcgca
     1441 cgatgtcggc cgcgcccatc gcaccgggtt ggcgcgcaat ctcgcgcccg tgctggaaca
     1501 ggaccagggt ggggatgctg cggatggcga atttctgcgc cagggtcggc tgcgcctggg
     1561 tatcgacctt ggccagccgc acccgcggct ccagttgcgc ggccgcggcc tcgaactgcg
     1621 gtgccatcgc gcggcacggg ccgcaccagg gcgcccagaa atccaccagt accggcaggc
     1681 cggcgcgttc gacatgcgcg tggaagccag cctcgtccaa cgccagcggg gccgcggcga
     1741 acagcgcgcg atggcactgg ccgcaattgg gagcctcgcc caagcgcgcc tgcgggaccc
     1801 ggttcagggc attgcagagg ggacaggcga cgatcatggc ttcgttcatg gcgtgattcc
     1861 gctcagtcca gtgcgggcac ttccacccac agcgcgccgc gcaggtcgcc ggcatcgaaa
     1921 ccggtggcgg tatcgccggg atacagccgc tccagcgcgg cgcgggtatc gtcggccagc
     1981 gccttgccgt ggcaggcaag gcacgccggc tccaccggga tgccgcgcat catccgcagc
     2041 gccacgccgt cgggcaggcc gctgtcgacc acctgcacca gctccgcgac cggcgtgccg
     2101 gcatcgtggc gtgcgatcaa cgcgcgcagc gcgtccgcct gccacgcgtc cggcgcattg
     2161 gccgggctgc gctggcggcc cggcaccggc acccggccga tgcgcacgcc atgttcggcg
     2221 gcaacctgcg cggcgatctt cggtgcctgc gcgtggcaga aggtgatggc accggtggtg
     2281 ccacccttgg ccagttccgc gccaagcgcc tgcttcagcg aactgctgaa ggccttggcg
     2341 gccgcttgcg cccgcacttg cggcgcggtt ggcgcgggtg cctccaacgg cgccggagcg
     2401 gcaggtacgg ccactgctgc ggcgggttgc ggcggcggcg cgctggagca ggcggccagc
     2461 aggaccgcca gcagtggcgg caggcacagg gtcgtgcgga tcatgcgtgg acctcctgca
     2521 cccggcgccc ggtgcggcgc aggagcacgt agtagagcaa cgggatcacc accagcgtca
     2581 gcaccgtgct gaccaggatg ccgaagacca gcgacaccgc cagcccgttg aagatcgggt
     2641 cgtcgaggat gaagaacgcg cccatcatcg cggccacgcc ggtcagcacg atgggcttgg
     2701 cgcgcaccgc gcaggcatcg atcaccgcgt ccaccagcgg caccccgcga tccacttcct
     2761 ggttgatgaa atccaccagc aggatcgagt tgcgcacgat gatcccggcc agcgcgatca
     2821 tgccgatcat gctggtggcg gtgaactgcg cgcccagcag cgcatggccc ggcatcaccc
     2881 cgatcacggt cagcgggatc ggcgccatga tcaccagcgg caccaggtag ctgcggaact
     2941 gggcgaccac cagcaggtag atcaggacca tgccggcggc ataggcgatg cccatgtcgc
     3001 ggaaggtttc gtaggtgatc tgccactcgc cgtcccactt gatcgcgaag ccgctggtgt
     3061 cggccggctg ggcgatgaag gactgcgcga cctggtggcc gccgatggcg tcggcggaca
     3121 cctgcccgac caggtcgaac atgccgtaca gcgggctgtc gagctcgccg gcctcgtcgc
     3181 ccatcacgta caccaccggc agcaggtcct tgtggtggat cgcgccgtcc cacggcgcgc
     3241 gccggaccgc gaccagctcc gacagcggca ccagctggcc ctggccaccg cgcaccttga
     3301 gtgccagcag gcggtcgacc gcggcctgtt cggcgggtgg cagccgcagc cgcaccgggc
     3361 gcggatactt ggaggcgccg tcgtggacgt aggttgcatc cagtccggcg accgcggcgg
     3421 ccagcgcatc gctgatcgcc gcctgcggca ccccgagccg ggtggcgcgc gcgcggtcga
     3481 ccacgatcac ctcgcgcggg gcgtcggatt ccacgctggt gtcgacgtcg acgatgcctt
     3541 cggtcgcgag gaagcgggct tccagcgcct tgccgagctt gcggctggcg gcgtagtcgg
     3601 ggccgtagac ctcggccacc agcggcgaca gcaccggcgg ccccggcggc acctcgacca
     3661 ccttcaccga ggcgtcgtgg cggcgcccga tctccgccag ccgcgggcgc agttcgcgcg
     3721 cgatggcatg gctctggcgg tcgcggtggt gcttgtcgac caggttgacc tgcaggtcgc
     3781 cgacgttgct gccgctgcgc aggaagtact gccgcaccag gccgttgaag ttgatcggcg
     3841 cggcggtgcc ggcatagccc tggtagtcca gcacctcggg catgcgctcg accaccgccg
     3901 ccagttcgac cagcagcgcg ttggtgtcct ccagggtgcg tccctcgggc aggtcgacca
     3961 ccacctgcag ttcggacttg ttgtcgaacg gcagcatctt cagcaccacc agctggaaca
     4021 cggccagcga tgccgccagc gccaccagcg ccaccatcga ggcgaacagc agcccgcgct
     4081 tgcggccggc accggcaccc agcaggaacg gcgacatcac cctgcggaac agccgatgca
     4141 ggcggccctg cggctccccg tcgggtgcgt gatccgcttc gccggcatgg ccgtggcggg
     4201 cgagcagctt gagcgacagc cacggcgtca cgatcagggc gatcgccagc gacagcagca
     4261 tgccgaccga ggcgttgatc gggatcgggc gcatgtacgg ccccatcagc ccggacacga
     4321 acgccatcgg catcagcgcc gcgatcacgg tgaaggtggc gaggatggtc ggcccgccga
     4381 cttcatcgac cgccggcggg atcgcttcgc gcagcgactt gccgccgcgc gccatgtgcc
     4441 ggtggatgtt ctccaccacc acgatggcgt cgtcgacgag gatgccgatc gagaagatca
     4501 gcgcgaacag cgacacccgg ttgagggtga accccatcgc ccacgaggcg aacagggtca
     4561 gcgccagggt cagcaccacc gcgctgccga ccacgatcgc ctcgcgccag cccagcgcga
     4621 acagcaccag cagcaccacc gaggcggtcg cgaacgccag cttctgcatc agcttggcgg
     4681 ccttgtcggc ggcggtccgg ccgtaatcgc gggtgaccgt gacctcgacg ccctcgggga
     4741 tcaattcgcc cttgagctgg ccgatacggc gtgcgactgc gctggtgatg tcggcggcgt
     4801 tgctgcccgg cttcttggcg atcgcgatgg tcaccgcggg ggcggcgccc tgcgcgggac
     4861 cggccttgcc ggcgggtgcg ccgtgccagg cgtagcggct ggcgatgtcg ccgccggcgt
     4921 gcacctgggc cacgtcggac agccgcagcg gcctgccgcc gtcgagcccg atcaccaggt
     4981 cgcgcacgtc ttcggcgctg gcgaggaagg tgccggcggt gaccggcgtg gccaccccgc
     5041 cacccacgcg ctcacccacc tgctgcaccg cattggcggc gcgcagcgcc tggccgaggt
     5101 cggccacggt gaggccgtgg ctggcgaggc gggcgggatc cagggtcacc gcgaccaccc
     5161 ggtccggggc accgatcgta tagacgtcgc gcgtgcccgg gatgcgcttg agttcggttt
     5221 ccagggtgtg tgcgacttcg gcaagcgcgg tggcgccgcg cgcgggatcg tcgctccaca
     5281 gggtcagtgc catcaccggc acgtcgtcga tgcccttcgg cttgaccagc ggctggccga
     5341 cgccgacgcc ctgtggcagc cagtcctggt tggaatagac ctggttgtac aggcgcacga
     5401 tcgccggctg gcgttcgacc ccgaccgtgt actcgacggt gagcaccgcc atgcccggcc
     5461 ggctgaccga atacacgtgc ttgacgcctt cgatctccga cagcttctgt tccagcggcg
     5521 tgctcaccag ttgctcgacg tcgcgcgcac tggcgccggc gaacggcacg aacacgttgg
     5581 ccatggtcac gtcgatctgc ggctcttcct cgcgcggggt gaccagcacc gcgaccagtc
     5641 ccagcagcag gccgagcagg gccaggatcg gcgtcagcgg gttggcctgg aacgcggcgg
     5701 ccaaccgacc ggacaggccg aagcggcgcg gttcagtcat cgccccgctc catcgccttg
     5761 cgctgcgcca ccagcgcctg cactgcggcc accggatcgg cggccacgcg ctcgccgggc
     5821 ttgagcccgg ccagcacctc gacgcggtcg ccgctgcgct ggcccagccg cagctggcgc
     5881 aaggcaacct ggttgccggc gatgacgtaa acgccactga cctcgccgcg ctgcatcagc
     5941 gcgctggccg gcaggctcac cgccgaaggg gccgcattcg tggcgatcgg gaacaccacc
     6001 ttcgcggtca tgcccggcgc gggtgcaccc ggcatgtccg ccagcagcac gcgaacgccg
     6061 acgctgtggg tgaccggatc ggcggcggga aacaccacca cctgcgcggc atcgaaggct
     6121 tcgcccccgg cgagttcgat gcgggcctgc ggcctggcgc ggatcgcatc ggcctgcgac
     6181 tgcggcacct gcacctggat ccgcaatgcg ccgggcgcgt acaccgccag caacggctgc
     6241 cccggggcga cgctctcgcc cggctccacc aggcgcgcgc tgacgattcc cgcatacggc
     6301 gcgcgcacca ccgtgtagtc agcctgctgc gcggcctgcg caagttgcgc gcgggcagcg
     6361 tcgcgggcgg caacggcact gtcgcgggcc gcgcgggcct ggtcgacctg cgcgcgcgac
     6421 acgaactggc cgccggccag cgcgctgaag cgacggtagt tggcctctgc ctccgccgcc
     6481 gcagcctcgg cggcacgcac ctgcgcgcgc gcggtgttgg caccggcctg ctgctccacc
     6541 gcggacagcc gcagcagcac ctggccctgc gccacccggg tgttgacgtc gaccagtacg
     6601 gcggtgaccc ggcccgcggt ctgcgcggtg agttcggcct ggcgcaccgc ctcgaccacg
     6661 ccatcccagc ggctgccggc accgttcgag gccgccgtga cttcgatggt gtcgagcgcg
     6721 ggcaatgcgg gcgtggtggt gcccgccggt tcgccgccgc aggccgcgag ggccagaaaa
     6781 ccggtggcga gcagggtctt gcgcaagtcg atcatcgtgt cggtccgtcc atcgtcattt
     6841 cgtttggcgc acgcacccgc cgcgcgccgt ggcacgcggc gggcctggcg gcgatcagcg
     6901 gcagcagctg gcgtcgttgg cgttgcggcc gatgccgagc ttgcgcatca ccattcccgc
     6961 cgggcagaag ccggtgaacg ccgattggaa cagattgaag ccgatgaacg cggtcagcca
     7021 gaagaaattg ggatggacga actgggtcag cagcacgctg accaccacca tcacgccggc
     7081 aaacgcctgt acggctcgat cgagggtcat gtcatttctc cttgggggtc tggattcagc
     7141 tggaacgctt gacgtccttc agccgctcga tgccgagcgc gctcaggacg tatttctcgt
     7201 agatgggttc ggaggtgccg ttgcgcatct tgtagaggaa gtatttctcg aacgcgatct
     7261 tggccaggtg cacccacttg ccgatcttgg tccacgtgac attgcgcggc gggatctgcg
     7321 gcagcgccac gaaggcggcg ccggtatcgc ccatgtcggc caggcagacc gcgttccagg
     7381 tgccctcgaa gtcgggagcc tcgcccttca gctcggcctg gatgttgcgc acgatggtgg
     7441 tgaccatcga ctcgatcatg aagccggtct tgggcgcgcc ggtcggcacc ggggtggcct
     7501 cgaccggggg tatcgccacg catacgccgg ccgagaagat cttcgggtac ttcgggctgc
     7561 gctggtgctt gtccacgatc acgaagccgc gcgggttgca caggccttcc accgccgcca
     7621 ccggctccac gcccttgaac gccggcagca gcatcgagaa cttgaacggc agcacgtgcg
     7681 attcgccggg ctggcccttg gcatcgtgct cgaccacggt catctcgccg tcgcgcacct
     7741 cggccacctt gctgttgacg atccacttga tgtggcgctg gcgcatctcc gattccatca
     7801 ggcccttgga atcgccgacg ccgcccagcc ccatgtggcc gatgtagggc tcgctggtga
     7861 cgaaggtcat cggcaccttg tcgcgcagct tgcgcttgcg caggtcggca tcgacgatca
     7921 tcgcgaactc gtaggccggg ccgaagcagc tggcaccggc caccgcgccg atcaccaccg
     7981 ggccgggatc ctgcaggaat gcctggtagg cctcccacgc gtgcgcggcg tgcggggtgg
     8041 tgcagaccga ctgggtgaag ccgccgtccg ggccggtgcc gggcacttcc tcgaaggcca
     8101 gcttcggacc ggtgcagacc agcaggtagt cgtaggggat gcgctcgccg ccgccggtga
     8161 tcaccgcgtc gccttcggca tcgatgcgtt cgacgccatc accgttgaac gcgatgccgt
     8221 gcttgccgac gtgtccggcc acgtccaggg tgatctgctc ctgctggcgc caacctactg
     8281 ccagccacgg gttggacggg gtgaagccga actggccgcc cttgcccacc agcgtgacct
     8341 cgtgcccctt gcccagcacc tcgcgcagct cgtatgccgc gctcatgccg cccagccctg
     8401 cgcccatcac cacgatcctg gccatttacc atctcctgcc ggtttgtgct ggaattaata
     8461 ttagataccc cttatattag tgtcaactga tatttcaagg agtcccgatg accaagttcc
     8521 tcaccgccgc cgacctcgtt gccgaagccc aggcgcgcat ccgcgaagtg ccaccgcagc
     8581 ggttcgaggg cgggttcggc gacgcggtgc tgatcgacgt gcgcgaacct gccgaattcg
     8641 aaaccggcca catccccggt gcgatcaaca tcccgcgcgg cgtgctggag ttccaggtgg
     8701 acgcgcatcc ggcggtggcc aatgtcagcg acccggcgct ctcgcacagg gagcgcccgg
     8761 tcgtgttggc gtgccgcacc ggcggccgcg ccgcgcttgc ggcagacaac ctgcagcggc
     8821 tcggtttcgc cgacgtgcgc tcgatcgccg gtggcgtgct gggctgggcc gaagccggcc
     8881 tgccgctggt ggtacgttaa gcggatgacg caggcacgcc gcaaacccgc gccgccggtg
     8941 ctggatccgg aatcgatgcg cgcgcacgcc gccgacgcca gccgcctgct gaaggcgctg
     9001 gcgaacgaca agcggctgat gctgctgtgc ctgctgtccg agggcgaacg ctcggtcggc
     9061 gaactgaacg cgcgcgtcga cctcagccag tcggcgttgt cgcaacacct cgcggtgctg
     9121 cgcgaggacg gcctggtgac cacgcgccgc gacgcccagg cgatcctcta ttcggtcgca
     9181 cccggccccg cgcaacgcat catcgcaacc ctgcacgaca tctactgcgg caacgaccct
     9241 cctttctgcg agtgaatgcg atgggcgcgc ccctgcagat cgagccattc ctgcatgccg
     9301 gcagcggcac ctggacccat gtggtccacc gcggcgatga cgcggtggtg atcgacccgg
     9361 tcctggacta cgacgccgct tccgggcgga tcggcgtcgc aggcctgcag gcggtgcggg
     9421 actacctcga cacgcacggc ctgcagctgc gccgcatcct cgagacccat gcccacgccg
     9481 accacctgag cgcagccgcg gtgctgcgcg cggccaccgg cgcgccggtc gggatcggcg
     9541 acggcatccg ccaggtgcag gcgcatttcg cgcaggtgtt cgccatcgat ggcgacgacc
     9601 cggccctgcg cgatgcgttc gacgcgacct tccgcgacgg cgacgcgatc gaggcgggct
     9661 ctctgcgctt ccatgtgctg gccctgcccg ggcacacctc ggacagcctg ggctatgcga
     9721 tcgacggcca cgtgttcgtg ggcgacagcg tgttcgcacc cgacatcggc accgcgcgct
     9781 gcgacttccc gggcggcaac atgcaggcac tgcatgcctc gatccagcgc ctgcatgcaa
     9841 tggacgaggc caccgtgctg cacctgtgcc acgactatcc gcccgccgga cgcggcccgc
     9901 gcagcagcgt gaccgttggc gagagccggc gcgacaaccg catgctgaat gccggcacca
     9961 cgctggaggc gttcgtcgat gcccggcgcg cgcgcgatgc gcagctggcc gcaccggcgc
    10021 tgctgtatcc ggcgctgcag gtgaatatcc gtggcggtcg gctgccgccg gcgcaggcga
    10081 acggacgcca ttacctggcc acgccactgc agggcgagct gcccgcgggg cttgcctgag
    10141 cgcgccgctt gcgctggcgc cggccgcgct ggcacgctcg cgctccgtcc gccaggccgc
    10201 accgcttcga tgacctcccc cgatctcctg ctggccgcgc tgttcttcgc ggtcgccgtg
    10261 ctgtactcct cggtcggcca cgccggcgcc agcggctaca tcgcctcgat ggccctgctc
    10321 ggcttcgcgc cggaacaggt gcggccgacc gcactcgccc tgaacctgct ggttggcggc
    10381 atcggcctgt accgctggtg gcgcggcggc cacgtgcgct ggcgcaacgt gctgccgttc
    10441 gtgctggcgt cggcgccggc ggccttcttc tccgcgcaga tcaagttgcc gaaggaaagc
    10501 tattcgctgc tgctgggcat cgtgctgctg gttgccgcga tcggcgtgtt ccgccatgca
    10561 tcgcgtgccg agagcgaaga cgccgccgcg cagggccgaa acgtgccgtg gctggccggc
    10621 ctgctggtcg gcgcggccat cggcacgctg tccggcctca ccggcaccgg cggcgcgatc
    10681 ttcctcaccc cgctgctgct gttcgcgcac tggatgccga cccgcgaagc cagcggcacc
    10741 tcggtcgcct tcgtctggat caactcgctg accgggttgg ccggcctgct gcactccggg
    10801 cagtcgctgc cggcgatgct gccgtggtgg ttgggcgtgg tcgcgatcgg cgcgctgatc
    10861 ggcacccaga tgggcttgaa gtggctgccg gtgcgcgggc tgcgttatgc gctttgcgcg
    10921 gtcctgttga tcgcgtccgg gaagctgctg ctggcctgat cgccaccaac gcaagcggcg
    10981 cccgcaggcg ccgcttgccg agaccctgga acgcagtgcc tacagcgcct gcgccgccgc
    11041 gaccaccctg tccacggtga agccgaaatg cggcagcagc gcctcgatcg gcgcggaggc
    11101 accgaagctg tcgatgccga ccaccgcgcc gtccaggccc acgtacttgc gccagaaatc
    11161 gctgacgccg gcttcgatcg ccacccgctt gcggcaggcc ttcggcaaca ccgcttcgcg
    11221 atatgcggcg tcctggcggt cgaacacgtc ggtcgacggc atcgagacca cgcgcacgcc
    11281 atcgcccaac tgcgcggccg cctgcatcgc cagtcccact tcggaaccgg tggcgaccag
    11341 gatgacctcc ggcgcgccca cgctgtcctt cagcacgtag ccgccgcgcg cgatggcggc
    11401 cagctgcgca tcgctgcgcg gctggtgcgg caggttctgg cggctgaaca ccaggcagct
    11461 ggggccgtcg gcgcgctcga tcgcggcctt ccacgccacc gccgattcca ccgcatcgca
    11521 cggacgccac acatcgttgt ccgggatgta gcgcagcgag gcgaggtgct cgatcggctg
    11581 gtgggtgggg ccgtcttcgc ccaggccgat ggagtcgtgg gtgtacacgt ggatcgcgcg
    11641 cacgcgcatc agcgcgctca tccgcaccgc gttgcgggcg tagtcgctga acaccaggaa
    11701 ggtggcgtcg tacgggatga agccgccgtg cagggccagg ccgttgctga tcgcggtcat
    11761 gccgaattcg cgcacgccgg aatagatgta gttcgcgttc gggtcgtcgg tcgcgaccga
    11821 cttgctgccc ttccacaggg tcaggttgga atgcgccagg tcggccgaac cgcccaccag
    11881 ttccggcagc agcggcgcga acgcctcgat ggcgttctgc gaggccttgc ggctggcgat
    11941 caccgggccg tcctgctgca gcctggcgat gaaggcatcg gcatcggcgc caaagccgcc
    12001 gggcagttcg gcggccatgc gccgctcgaa ctcggcggcg gcgtcgccat gcgcggcctt
    12061 gtaggcggcg aaacgcgcgt tccacgccga ttcggccgcg gcgaggtcac gcgcgcgcca
    12121 gccggcgtac agcggctgcg ggatctcgaa cgcgccatgc gtccagccca gcgcgtcgcg
    12181 ggtggcctgc acttcgtcct tgcccagcgg cgaaccgtgg ctggattcct tgccggcctt
    12241 gttcggcgaa ccgaaaccga tcgtcgtgcg gcagcagatc agggtgggct tgccggacga
    12301 ctccagcgcg gtctcgatcg cggtccggat ttccaccgga tcgtgcccgt cgacgccgcg
    12361 caccacgttc cagccgtagg cctcgaaacg cgccggggtg tcgtcggtga accagccgtc
    12421 ggtgttgccg tcgatgctga tgtggttgtc gtcccagaac gcgaccagct tgcccaggcc
    12481 ccaggtgccc gccagcgagg ccgcttcgtg gctgatgcct tccatcaggc agccgtcgcc
    12541 gaggaacacc caggtgcggt ggtcgacgat ggcgtggccg tcgcggttga agcgctgggc
    12601 cagcaatttt tccgccagcg cgaagccgac cgcgttcgcc agtccctgcc ccagcggtcc
    12661 ggtggtggtc tcgatgcccg cggtgtgggt gttctccggg tgccccggcg tcttcgagcc
    12721 aagcttccgg aacgccttca gctcatccag cggcaggtcg tagccggcca ggtgcagcag
    12781 cgcgtactgc agcatcgagc cgtggccgtt gctcaggatg aaacggtccc ggtccggcca
    12841 tgccgggttg gccgggttgt gcttgaggta gtcgttccac agtacttcgg cgatgtcggc
    12901 catgcccatc ggcatgccgg gatggccgga gttcgcggcc tggaccgcat cgatggcaag
    12961 gaaacggatc gcgttggcga gatcgcggcg gctgggcgag gtcatggggg cgtcagggaa
    13021 gcgcccggcg gcgtgcgggc ggcgcattgt cccatcccgg caccggccgc cgccagcacc
    13081 agccggcccg cgaggtcagc gcacgatgtc gtccagctgc gccagcgcct cgtccggcag
    13141 gtccttttcc ccgctccggt gcgcgaccac gacagcggtg gcgaggtcgc cggtgacgtt
    13201 cagcgaggtc cggcacatgt cgaggaaggt gttcaccccg aggatgatgc cgatgccttc
    13261 aggcttgatg ccgatgatcc cgcagatcat cgcgatcacc ggcagcgagc ccgaggggat
    13321 cccggcggtg ccgatgccgc ccaggatgca aagcgcaagc accatcagct gctgcggcag
    13381 gcccaggtgc aggccataga cctgggccag gaacagcacg gtgatgccct cgaacagcgc
    13441 ggtgccatgg tggttggcgg acgcgcccac ggtcagcacg aaccgcgaca ccttccgcgg
    13501 caacttcagg ttttcctcgg ccacgcgcag cgtgaccggc agggtcgcgg tggacgaggc
    13561 ggtggcgaat gcggtcagca ccgcttcctg ggtgccgcgg aagaaggtgc gtggcgccat
    13621 gccgcccatg aaccggaccc acagcggcaa caccacgagc ccatgcaggc ccagcgccag
    13681 cagcaccgtg agcacgaacc acagcaggct ccggatcaca tcccagccga gctgcgaggt
    13741 gaggttgaac atgaatgccg ccaccgcgta cggcgccagc ttgatgaaca tcccgatcag
    13801 gtgcatgcag atctcgaaca ggccgtgcaa cgccgacttg aacgcacgcg tgcccggggt
    13861 cggcttcatc accaggccga tgccgaccat cagcgcgaag aacacgatgc cgagcttctg
    13921 cttgtcgtca cccatcgcgc cgacgatgtt gtgcgggacg atgttgacca gcaggtccat
    13981 caaccgcagc tcactgccct tgctcacgat ttcaccggtg cgggcgttgc tcatcgcctg
    14041 ttcgaccagc gccgggtcca tgtgggtgcc cggctggaac aggttggcgc aggccaggcc
    14101 gatcccaacc gccaacgcgg tcacgaccgc ggtgtaggtc agcgtcttcc agcccagcct
    14161 gcccagcgat gcaatgtcgc ccagctcggc caccccgagc accagcgcgg agaacatcaa
    14221 gggcaccacc agcatgaaca gcaggttgag gaacacctgc ccgaacggct tggtcgcgta
    14281 ttcgatcacc ccctggaccc aggccgcatc cgccgccagc gcatgcaccg caagcccggc
    14341 cagggtgccc agcacgaagc cgagcaggac cttcacgtgc aggggccacc cacgggaggc
    14401 cacgggcgaa gcggaggcgg cggaatgcat gggatgcaac tcgttggaga cgaagcggcc
    14461 agcttagcaa acgcgccccg ggctaccgcg gatacaacgg cggcagcagc gacgatggcg
    14521 cgcgcccccg catgcgccag cgcggacctt gcgcgaatgc accgcgcagc gccaccagcg
    14581 cgaccgccgc atcgccatcg ccccaacgcg cggcctgcag cctttcgatc gcttcgcgct
    14641 gggcggtgtc gtcgagcctg gcgcggacgc cgtcgaggtc atcaccccgc acgccggctt
    14701 cgccggaaag tgcccgcgcg atggcggaaa gttcgccctg ccgcaaggcc tctgccagcg
    14761 acggtgacct cgcagccgct ggctgcggcg atgcgcccgg aacctcggcc atgcgcacgc
    14821 ttgccctgcg cgcgcgcgac caccaccagg tgaacgccgc gatcaccgcc agcgccaccg
    14881 cggccagcaa caaccagcca cggcggtcgc gcacgggcgc cacggccgaa gcgcgcggat
    14941 ccggcgtttc gccagccgtg gctggcgccc gcggaacttc gccggacccg acgggcgcgt
    15001 ccagcgccgg cgaaacctgc aactgcagcg gcggcagcat ggcggtgcgc gccaccccct
    15061 gccctgcgtc ccaccattcg atgcgcgggc ccggcaggga caacgcgccc ggcttcaacg
    15121 gcacgatcgc gatgcgccgg cgcagggtgg tgcgcgggcg gccctcgatg aactgctcgt
    15181 ccgcctgcac cgggtcggcg aacacctgcg cctggtccgt ctccgccaat cccggcaccg
    15241 gcatctggct ggcgcgtgca ccgtcggcga tcgcttccag ctcgaccatc gtcgcctcgc
    15301 ccacgcgcgc atcgcgcggt gactgcaggt aacgcagcct gaggtcgcgc aacggcagcc
    15361 acggctgcgg cgcgtcggcc gggaccggcc ggacctgcag gcgctggtcg ggggcggccg
    15421 ccgccagcgg cttgcggccg tcgtcgaaaa tgttgtcgaa gaagccgccc accgcctggc
    15481 cgttgaagcg cgcgcccggc acccgcagcg gcccgctgcg ttccggcacc agcaggtagc
    15541 gacgctcgac cacgctgtag cgacggccgc cgatctcgcg ctggtactgg atgtccgcgc
    15601 ccacctgctc caggctggcg ccttccacct ggtcctggtc gagctggccc gacagcaccg
    15661 gcaccgccac gtgcaggcgc acggccatgc ccaccgcctg ctgcacgtag ggctgggtcg
    15721 catcgaattc ggtctcgacg aaggcctcgg catcgccgcc ggcctgctgc accgttggtg
    15781 gcagcacggt gagccgcaac ggcgaggtgc tggcgttgcc gacgcgcagg gccggcacgg
    15841 tgatcacccc cggcccgcgc gggcggatgc cgaccgcgaa cagcgacttc gcccgtgact
    15901 gcccgttgac catcccgaag ctgcggcgca cggtctgtcc ggcgatgtcg aactgcgccg
    15961 ccagcggacg gtaatcgacc tgccgtaccg aggcactggt ctcgatgttg agggtcacgg
    16021 tctcgccata cgtgacctgc gcgcgatcca gccaggcgcg ggtctgcgcg gacacgccgg
    16081 tcaccagccc tggcaatgcg cacagcgcga ccagcaagcc gaaggcgcgc gcgcggttca
    16141 ttgcccgagt ccctgccggc gctggtgttc aagctggaac ttggcgcgca gcagcgcacc
    16201 ggggtcgtcc ggcacccgct gcagctgtgc ctggttggcg atccggcgct cgcgctgctc
    16261 cggggtttcc gccggctgcg ggcgcggctg gcccggcacc tgcgattggc cttgcttgcg
    16321 caaggcgtcc tgcatgcgct ggcgttgggc cgcatccgcc tgctgctgtt ggcgcggatc
    16381 ggcggccttg tcctggctgc cctgcccggg ctgccgcgat ggatcgccct gcggccggcc
    16441 cggctgccgg gactgctgcg catcccgttg ctggccctgg tctcgttgct ggtccccgcc
    16501 ttgttgcggg ccctgttgcg actgcccgcc cggcggcggc ttgcgcttgc gcgccgcatc
    16561 caccgcggcg cgattcgcga ccgcatccgg catccccggc cgctgctgca gggcgcggtc
    16621 gtaggcggcg atcgcctggt cgtagcggcc ggcctgcgcc agcgcattgc cgaggttgta
    16681 ctggccctcg gcactggcgt tcccggtgaa tttcgcgatc gcctcctcgt attgctgcct
    16741 gcgatacgcg gccacgcctt caagcatccg cgcatgcgcc tgctggtcgg cgcggcgcca
    16801 caggccactt gcgtcctgcg cctgcgccgg cggcatcggc aaccacaaca ccagcagcag
    16861 catcgccatc gccgcgccgc cgcgccggaa caacggcagc gcgaacagca gcgcgaatgg
    16921 cagcagccag gcgccatcat cgcgccagcg gcgactggtg ccgccgcccg ctgcagcggc
    16981 acccgcatcc tgcggatcga gcacgtccag cgcacgcagg tcgtggttac cggccgccag
    17041 cggctggtaa cggccgccac cgctggcggc gaggcgttgc aggctggcgg cgtcgagccg
    17101 cgcattgccc agcccgccgg gggtgtcgaa tgcgccgcca cccgcagtgc ccagacccag
    17161 cgcggacacc cggtagccgg cggcgcgcgc gcgcgcggcc tcggcgatgg cctggccatc
    17221 ggcgcgatcg gtgagcagca ggacgtcgcc ctgggcgaac ccggcattgc gcagcagccg
    17281 ctgcgaccag gcgatcgcac ggtcgccacg gtggccgtcc gcgggcatca cgtccggtgc
    17341 cagcgcatcc aggaacagcg ccacgttggc ggcatcctcg gtcagcggcg ccaccgtgta
    17401 ggcgtcgtcg gcgaaggcaa ccagcgccac ctgcccgccg gcgcgttcgc gcagcaaggt
    17461 ggcgatcttg gcccgcgcct gggccagccg cgagggcggc aggtcgcggg ccaggatgct
    17521 gctggacagg tccagcgcga ccaccagcgg cgccttcgcc tgccacagcg ggtgttcggc
    17581 cttgcccacg cttgggcccg ccatcgcgat caccgccagt gccgccgcca acatgcccga
    17641 gccacgcgcc agccgggcac gcggcgaccc ttccgcggcc accagcaggt gcggcaacag
    17701 gtgcggatcc actgcgtcct gccacgcgct gcgcggttgc gcgcgcgcgc gccaccacca
    17761 cgccagcagc ggcaatgcga gcaggcagag cagccaatac gggcgcagga aatgcacggg
    17821 cagcgctgca agcaggccgc tcaccggcgc ctccccacga ccagcgccag caggccaatc
    17881 cccagcgccc aggccaacgg cagcggatag cgctcgatct tcggccgcac cgcctggcct
    17941 tgccgctgca ccggttccag cgcgtcgatc tcggcgtaga tgccggccag cgcgtcggtg
    18001 tcgcgggcac ggaagaagcg cccgccggtc accgcggcga tccgctgcaa acccgcctcg
    18061 tccacctcgt cgccgcccat cggcagctgg aaaccgaaca ccgacagcgc gccgccatcg
    18121 ccgccaaacg caaccgcgtg gatgcgcacg ccgttgtcgc gcgcgatctg cgccgccttc
    18181 gccgggtcaa gggtaccggc ggtgttgacc ccgtcggtga gcaggaccag cacccgctgg
    18241 ccgtctggct gctgctgcag gcgcttggtt gccagcgcaa tcgcgtcgcc gatcgcggtg
    18301 gcgcggccgg ccaggccgac cacgctgtcg ccgagctgct cctgcacgct ggcgcgatcg
    18361 agggtcagcg gggtcaaggc gaaggcgcgc tcgccgaaca ccaccagccc gacccggtcg
    18421 ccggcgcggc gttcgaggaa atctgccagc accgccttgg ccgcggtcag gcggtcgacg
    18481 atgcgcccgc cgagttccat gtcctgctcg cccatgctcg ccgacaggtc gaccgccagc
    18541 atcaggtcgc gcccgacctg cggcggggcg atcgccgggc cgagctgttg cgggcgcgcg
    18601 gcggccacgc acagcaggct ccacgcgagg aacgccagcc acgcaaagcc gcgtggcgtc
    18661 ggcgcaagtg aaccaccgct ggcgatgcgc agcaggcggt cgttccacgg cacccgcaac
    18721 gccggctgcg cactgcggcg cgccgggacc agcaggtgca ccagccacgg cagcggcagc
    18781 gcgagcagca tccatggcca cgcgaaaccg tccaggccca gcagttcgcg cagcgcgttc
    18841 atgcccgcat ccagcgcagg tagcgctcgc gggcgaggtc gcgcagcacg gcgcattcct
    18901 gcgcggccac ctgcgggcgg aatccgccgt cgcccagcag gcgtccggca ccggtgctga
    18961 acaccggccg cggcatgccc tcgtcgagga agcgcagcca ctcgtccccc agcaggcggt
    19021 cggcacccgc gtgcttgcgc cgcgccgcgc gacgcagcag ttcggacatc gccgcgacct
    19081 gctgcgccgg agttccggca tgcgccagcg cgtcgtcgaa caggcgcagg atcgcctcgc
    19141 gccgtcgacg cttcgccgcc gcccgccaac caagcaatgt cgccacgatc acgatcgcac
    19201 ccagcagcag ccaccagccc ggcgcgggcg gccaccaggc cggcgctacc ccttcgtgca
    19261 catcgcgcag cggcagcggc gtcatcgtgg cctgggcttt cgatcgagca gtggtagcca
    19321 cgaatcgctg gggtccgcgc tggacagttc catcgcgcgg attccgcacg ccgccagtga
    19381 ttccagcgcg gcttccagcg gaatgccgaa ttcgtgccgc cagcgctggc gggtgatcgc
    19441 cgcgtccagc ccgagttcga cccggtcgcc ggcggcggat gcgaacgcca ggcgcgcggc
    19501 cggcggcgtc cgctccagcg gatcggtcag cagcagcacg atcacttcgt ggtgcagcgc
    19561 cagtgccgtc cagcgcgcga gcggcacctg gacgatgctg gccgcgtcgg ccagtaccag
    19621 cagccgggaa cccggccgca gcagccgccg ggcgtggtct agcgccaccg cgagtccgtc
    19681 gttggcagcc ggcggacggg cgtaccagcg caccagcgca tccagcaccc gcaaggcgcc
    19741 gcgcgggccc gacgccggcg tgaccggcgc ctcacccggt ccactgcgca gggcggcgat
    19801 gcggtcgccg tcgcgcaccg cgatccacgc cgccagcgcg ccggcacgcg ccgcctgtgc
    19861 cgacttgaag cgcgtgcggg tgccgaagta cagcgccggg gcggtatccg ccacgaccag
    19921 ggtgagccgc tcgcgctcgg cctggaacag cttggtgtgg gtgcgtccgc tgcgcgcggt
    19981 caaccgccag tcgatgtggc gggcatcgtc accctgcgcg tattcgcgcg actcggcgta
    20041 ttccatgccg cgcccgcgca ggttcgacag cgcgtgcccc tgcaggccat gccggccctc
    20101 gcgcgcgcca cggcgaccct gcaccagcgc gcgcaagccg atcagctccg gcagcgtggc
    20161 cgcggtcgcg gagccgcctg gcgcggcatg tccggtctcc gacaccgccg cgctcagggc
    20221 agcggaaccc gctgcagcag ctcggcgacc aggcgctcgc catcccagcc ttcggcggtg
    20281 gcctcgtagc tggggagcac ccggtggcgc aacacatcgg gcgccaccgc gcgtacgtcc
    20341 tcgggggtca cgaagtcgcg cccggccagc caggcacgcg cgcgcgcgca gcgttccagc
    20401 gcgatcgaac cgcgcgggct tgcaccccag gcgatccgcc gcgccaacgc agcgtcgtag
    20461 cgcgcggcat cgcgcgaggc cagcaccagt tcgaccaggt agcgttccag tgccggcgcc
    20521 acgtgcaggt cgagtaccgc ccggcgagcg gcgaacacgt cctcgatcgg gatccgcgcg
    20581 ggcgccggcg cgccagcctg cattgcgctg cgcgcgcgct cgcgcgccag ccgcaggatc
    20641 tcggtttccg catcgccatc gggatagccg atccgcacgt acatcaggaa ccggtcgagc
    20701 tgcgcttccg gcagcgggaa ggtgccttcc tgctcgatcg ggttctgggt cgccatcacc
    20761 aggaacagcg ccggcagcgg ataggtctgg cggccgactg tgacctggcg ctcgcccatc
    20821 gcctccagca gcgcggattg gaccttggcc ggcgcgcgat tgatctcgtc cgccagcagg
    20881 atcggatgga agatcggtcc cggcaggaac tcgaagcggc catcctgggg gcgccagacc
    20941 tcggtgccgg tcaggtcggc gggcaacagg tcgggggtga actggacacg ggcgaaaccg
    21001 gcatccagcc tggccgccag cgcgcggatc gcgctggtct tggccagccc gggcgcaccc
    21061 tcgaccagca ggtggccgtc ggcaagcaag gcgatcagca ggcgttcgat cagtcccgtc
    21121 tggccgacga tttcgcgcgc gaggtcctcg cgcagggcgc tgaaggccgc gtgcaggcgt
    21181 ggagtatccg cggtgggtgt atccatgggg tcctcgggtc gcggctggtt cgaccagcgc
    21241 tgcaggcgaa ggttcgcacg cccgcgcccg caggtgggtg aacgtcatgc cggtgccgca
    21301 aacaaagcgg gagcctttcg gctcccgtat tgcagtgcga tgcgtctgat cagcgctggg
    21361 cgacgcgcat caccctcggc gtcacgaaca ccagcagctc gcccttctcc ttggtgcgac
    21421 ccttgttgcg gaacaggttg ccgaggaatg gcaggtcacc gaggaacggc accttggcga
    21481 tgtcggaacg gtcccggaac tcgtagacac cgccaatcac cacggtctgg ccgtcgtcca
    21541 ccagcacggc ggtggtgacc tcgcgcttgg cgatctgcgg cacgtcgccg aacgtaccga
    21601 cgctcacgaa gccctccagt tcatccttct tgacggccag gttcatgaac acgcggccgt
    21661 cattggtgat ggtcggggtg accttgagct caagcaacac ctccttgaac tcaaccgtcg
    21721 gcacgttatt gctctggccg ccggtgaccg tcaggtagcc gatttcctgc ccctggcgga
    21781 tcaccgattc cttctggttg ctggtcacga tgcgcggatt ggagatcacc tcgccacgac
    21841 cctgctcctg gatcgccgac aattcgacat cgagcaggta cccggcattg aggatggaca
    21901 gcgccagcga accgggtgca ttggtggagc cgaagatccc ggccgccagg ttggtgatca
    21961 ggccgcgcgt ggtcgtactg ccgacgtagg tcggcggaac gcaaccgctc gggttcgcgc
    22021 cgccaaggca ggtgttgtag gaattctcca aggcgcggtt ggtctggctg gcctggtaat
    22081 ccgcgttcac gttggcggtg ttggtttcga gatcgccgct gaagtatccg ttctcgttgc
    22141 gaccgctgat gccgaaccgc gcacccagct cgcgtgccac cgattcggtg gcgaccacga
    22201 tgcgtgcctc gatcacgacc tgatcaaccg gcttgtccag tttctgcacc aggctgcgga
    22261 tttcggcgac cttctgcggg atgtcgatca ccagcagggt attggtgcgc ttgtcgaagc
    22321 tgatgctgcc gcgcgacgac aggaaaccac ggtcctggga gctctgcgcg ctctggccgc
    22381 cactgccgcc ctgcccgctg gaattgccct tgcttccctc ggtcagcagc ttggcgatgt
    22441 cctcggcgtt gccgtagttg atcggcatgt actccgtgac cttttcggca cgctcctcca
    22501 ggctgatgcg cgcatccgcc ttgtcctgct cgaactgggc gatctccttc tgcggcgcga
    22561 cccagacaac gctgccgctg cggcgcttgt cgagcgactt ggcctgcagg acgatgtcca
    22621 gcgcctggtc ccagggcacg tttaccaggc gcagggtcac gttgccctga acggtatccg
    22681 cggccacgat gttgaggttc gattcctcgg cgatcagctg caggacggtg cgcacgggca
    22741 cgtcctggaa gttgaaggtc accgggcgcc cgctgtagcg gatgctcgaa tcgctgtcgg
    22801 tcgcgcccat cgcggtggcg cccatcgccg gcgaggcggt ctttgccgtc gcgaccgggg
    22861 tggcgcgcgg cacgacctcg acgatgtatt cgttgccgct ctggtaagcc atcgactcga
    22921 acgtgccctg ggttcccagc accagttggg tcccgctgct ggtggcgcgc gcatcgatcc
    22981 gctgcaccgg ggtcgcgaaa tcggtgacgt tgatcgggcg ttgcagtgat gcgggcagct
    23041 gcgcgttgcc gatgtcgacg accacgctgg atcccatgtt gcgcaggtcg ggggccgcgc
    23101 cgtcgccgct gaagcgcagg atgagcttgc cggagccccc atcgccacgc ttgaagtcga
    23161 tcgcggtgac gctgaccgcg ccaggcgtgc gccgggccgg atctgccatt gcgccggtgg
    23221 ccgactgcgc catcgtcgcg gtggcgacta ccggcaatgc gccggcgccg gccgcgtaca
    23281 agcccactgc cagcccgact gcacaggctc cgagggacga ggggcgccgc aaaggccgct
    23341 gctgcttgac gttggtgacg gtcattggtc tatcccccaa tcaattgtct tcgagcgcca
    23401 tggtggccgg ccgttccagc catccacccg cgccatcagg aaccagttcg accagttcga
    23461 tccggtcttc gtagacggcg gtcacccgtc catcgctttg cccgaggtag gcaccgggac
    23521 gcacccggta ggtgaccttg tcgggcgcca gcaccagcgc cgcggtagcg gatccggctc
    23581 ccagtgtgcc gaccatgtcg aggctgtcca gcgggaattc ctcgagtgtc tgcttgcgcc
    23641 ggcccggatc cgggcgcggg ccgctgccgt tgaccccggt aaaagcctcg ctgaacgggt
    23701 cgcgcaggtt ctgcgcggcg tattcgaacg tctcgaactg ctgcatgacc ggcaacggct
    23761 caagcggcgg agccggcttg gccctgacct cggccaccca cttctcgagg tcgccccggc
    23821 cgctgccgca gcctgcaagc gccaccgcca tcgcgacaat gccgaccgcc cggaaggctt
    23881 ggatgccaat cattcgcatg tcaggcccct ccagtggcgg gcggggtgcc cgcggcgggc
    23941 ggcgtcgccg cggcggcctg cgccgcggtc tcgtcttcat ccaggtaccg gtaggtcttg
    24001 accgtgccgg aaagctcaag cgcgctgttc gggccgatcc tggcattcgg atcggtcttg
    24061 gcatccttcg gcttcagcga aatgtcgtgc atggtcatga tcaccacgcg cggcagcgag
    24121 gccacgccac tcacgaacgc accgaactgg tggtaggtcc ccaccatccg cagcgcgatc
    24181 ggcttctcgg cgtagaactc cttgggggtc tccggcccgg gctggaacag ctcgttctgg
    24241 atacccgtgg ccagcgcggt ctgggagatg tcgacgatca ggtccggcat ttcggtcttg
    24301 ctcggcagct ggcgcagcat ctgctgcagc tgctgctcca tctgcgcgag ctgctgcttc
    24361 agcggctcca ggttggccgc ccgcccctgc ttggtttcga actcggtgcg caattcgact
    24421 tcctgcttct gcaggccttc cagctcggtt cgccggtcgc gcacgaacag ccaccaggca
    24481 aggccgacga tcaacagggc gaggatcacg cagaacccga tcttggcatt ccgcggccag
    24541 gcaccgatgt tgttgatgtc cagttcacgc aggctgagct tcttggtgct cacggtgcag
    24601 tccctcctgc gacgggtgcc gccgcgacat cggcgggtgc ggtggcggct ggcgctgcgt
    24661 caggcttgcc atcgccgtcc tcgtccttgg gtgcgttcgg gttggccagc ttgaccttga
    24721 ggttgaactc gtagggcagg cccgcgttgc cggccttggc ctcgatgatg ttcaggtccg
    24781 gccctgtcat ccagccggaa ccttcgaggt tgcgcatgta ggtcgacacc cgcgcattcg
    24841 actgcgaacg cccgcccagg gtcaggatct cgccttcctg cttgatcgag gtcagggtca
    24901 ctccgtccgg gatggtacgc accagcgaat cgaacaggtg caccatctgc gatcggttcg
    24961 cttgcagctg ctcgatcacc tccttgcgcg caagcagctt gcccttcttc ttgtcgagtt
    25021 cctcgatctc cttgatctgg ccatcgacct tggtgatctc gccgcgcagg tattcgttgc
    25081 gcccggtctg gctgctgatc cgcccgctgt accagctgac gatcaggaac gacaccagca
    25141 ccccgcacag cgcggcgagg ccgagcatgc cgacgaattc cttctgccgg gccttgcgcc
    25201 gctcggcgcg ccacgggagg agattgatcc tggccatcag tcgaagctcc tcagtgcgag
    25261 cccgcaggcg atcagcaggg cgggcgcgtc ctgcgccagc gcatgcgcct gcacgcgtgg
    25321 cccgagggtc atctgcgcca gcgggttggc gaccgtggtc gccaccccca gctgctcctc
    25381 gaccatcgcc gcgatgcccg ggatggaggc gcagccgccg gccagcacca cgtggtcgac
    25441 gcggttgaat tcgctgcccg cgtagaagaa ctgcagcagg cggctgacct gctggaccat
    25501 cgcttcct
//
LOCUS       DAORUK010000010        25370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_1508_length_25370_cov_18.966068, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000010 DAORUK010000000
VERSION     DAORUK010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 25370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25370
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1508_length_25370_cov_18.966068"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /gene="murL"
                     /locus_tag="PK818_01400"
     CDS             complement(<1..1184)
                     /gene="murL"
                     /locus_tag="PK818_01400"
                     /EC_number="5.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011258235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-
                     glutamate epimerase"
                     /protein_id="HQY81422.1"
                     /translation="MSEWMFERDAIRTFRFVRCAFDAQTGIARLVYAFDDGPELVETV
                     TVPGAPFDLDDARAAAAGQALRLLHLVAGVSYYKAAVPTDIRIEAYEIDADTAAFLET
                     VYLNGLGEFAYRNGLDLRGRIRFPADAQAGVPAPALGLRGHALVAIGGGKDSLVSIEA
                     LRGAGVEQTVTWIGGSQLIAACAAHTGLPTLNIGRQLASQLFEYNRLGAWNGHIPVTA
                     VNSAILAFAAVLLGVDQVVFSNERSASYGSIIIADDGSVTGEVNHQWSKGWAFESALG
                     DWLQRHVAADLRYYSLLRPLSELAVARQFARVSRYDAHFSSCNRNFHILGERPTSRWC
                     GVCPKCHFVFLALAPFMPKPRLVGIIGRNLLDDPAQTAGFDALLEYQDHKPFECVGEG
                     RES"
     gene            complement(1181..3814)
                     /locus_tag="PK818_01405"
     CDS             complement(1181..3814)
                     /locus_tag="PK818_01405"
                     /EC_number="2.7.2.4"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/diaminopimelate
                     decarboxylase"
                     /protein_id="HQY81423.1"
                     /translation="MSIAAASDPTAPAQPWLVLKFGGTSVSKRERWDTIGQLAEGRAA
                     AGGARVLVVVSALSGVTNELQAISNGDDVKGRIAALVARHRAFCSDELGIDPDTAIGE
                     RLRALHALGDDPRAASRSLDWQAEVLAQGELLSSSIGAAYLRRQGLDFGWCDARDWLD
                     AVSLPNASDWAQRLSVNCRFDADAALRVRFAAQPAQLLLTQGFIARHPDGGTAILGRG
                     GSDTSAAYFGALLGARRVEIWTDVPGMFSANPRDVPDARLLTRLDYAEAQEIATTGAK
                     VLHPRSIKPCRDSGVPMRILDTSRPQLPGTRIEGSVAAVPGVKAISRRNGIVLVSMES
                     VGMWQQVGFLADVFERFKRHGLSVDLIGSSETNVTVSLDPSENLVSTDVLARLSEDLA
                     QVCRVKVIVPCAAVTLVGRGMRSLLHKLSAVWEMFGRERVHLISQSSNDLNLTFVVDE
                     AAADGLLHRLHAELIDSGAMPVYEAEVFGPRWREINGTVRPRPPAWWRAPRERERLLE
                     LAGHGTPRYVYHLPTVRARARQLQGIDAIDRRYYAIKANGHPALLRALAAEGFGLECV
                     SQGEVERVFEAVPGFDPARVLFTPSFAPIGEYEAALARGVTVTVDNVELLRRWPGVFR
                     GRALWLRIDLGHGDGHHAKVNTGGKDSKFGLSAQRVEEFVEAARALDVRITGVHAHLG
                     SGIETVDHWKGVVDELAGFARRIGSVETIDIGGGLPIAYSDDDEPFDLDAWAEGLAAI
                     KALHPAFRLAIEPGRFLVAEAGVLLAHATQVIEKDGVRRVGLDAGMNALLRPALYDAW
                     HDIVNLSRLDAGGDIDFDVVGPICESSDVFGQRVKLPAGTAPGDAMLVADAGAYGRSM
                     SNEYNLRALPAEDLLEEGMPE"
     gene            3903..4802
                     /locus_tag="PK818_01410"
     CDS             3903..4802
                     /locus_tag="PK818_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HQY81424.1"
                     /translation="MPRRYVQLDVFASRPGAGNPLAAVLDAEGMDTASMQALAAWLNL
                     SETIYFLPPDAGADYRIRIFTPGCELPFAGHPSVGAAWLAATHGLATPRDGQLVQQCA
                     AGLLPAQVHGDPLRPGILLRSPRGRLLEAATGALPRELAAIARPGQAVELWSNGPRWW
                     LLEAGSADALRGLKPDFGAIAGWTDATGSTGVAAFAFEDGGDHQLVVRAFCPGDAVGV
                     PEDPVTGSANAVIAARLHREGRLPGRDGRYIASQGRELGRDGRVTLQVDADGEVWIGG
                     EVQQVIAGSLEWPDAQATGPASP"
     gene            4799..5677
                     /locus_tag="PK818_01415"
     CDS             4799..5677
                     /locus_tag="PK818_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010369373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HQY81425.1"
                     /translation="MTRRRYVQLDVFASRAGDGNPLAVVLDAEGLDDARMQAIARWTR
                     LPETTFVLPPVATDATHRIRIFSPRREVPFAGHPSVGTAHALLDAGLAAPRDGLLVQD
                     GIAGKLPLMVTGAGRERTIAVRTPRARVQEIATPDDPRLRDALAGLPLGGLPPVLMDG
                     GRRWWLAELASEADLRNAQPNWEAIVALAHATDSMGLFVYARSQDPVHYFAVRAFVGA
                     PARFEDAASGAANATLAAWLAECGALPDGGFYRVSQGREVGHDAIIELRVDAGGDVWS
                     GGRAVAVVTGTLDWPD"
     gene            complement(5779..6474)
                     /locus_tag="PK818_01420"
     CDS             complement(5779..6474)
                     /locus_tag="PK818_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HQY81426.1"
                     /translation="MKRLAVALLVAVGLASGGTASAQSYPYPSGDGFNGQQQGEAYYD
                     YARVIRVDPVLDGRYGGGSSAVGQRCYETTSAGNDQRDGYDDVDGYRNDGYRNGGYYD
                     PYGRTQGGSMAGRNIATVVGGIAGAVLGSKVGGGSGTYAATAIGSMVGGMAGRGIYDQ
                     TQRDRYVRAGVVRVCDPEPAGGGYGSGYSGSGDGRASAYDVAYEYNGRRYTRRMDYHP
                     GDRVRVRVDVSPQ"
     gene            6807..8855
                     /gene="fusA"
                     /locus_tag="PK818_01425"
     CDS             6807..8855
                     /gene="fusA"
                     /locus_tag="PK818_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQY81427.1"
                     /translation="MSYSTQQIRNVALAGHPGAGKTTLFEALLHAGGAIQAAGTIERG
                     STVSDFDPIEKERGHSIDSAIASTDHAGIHLNLIDTPGYPDFRGPTLSALAAVETVAI
                     VVDADTGVSYGTQRMMEHAKARNLCRVIVVNKIDHPGAKLGALLDSLRETFGAEVLPL
                     NLPAEGGKSVVNCFWQSTGNSNLGAVADWHQKIIDQVVEINETVMDRYLDVGEGGLSG
                     QELHDAFEQCLREGHLVPVCFVSARSGAGVRELLDVAEKLFPHPGEANPPPFVKGTGD
                     DAQAITATPDPQAHVIADVFKIINDPFVGKLGIFRVYQGTVKKDTQLFVDDGKKPFKV
                     AHLFKLKGKDHVEVVQAIPGDIAAVAKIDEVHFDAVLHDSHDEDQIHLAPLAFPRPMF
                     GLAIEVARKGQEQKLANALHHLAEEDPCFHVEHELETNETVVRGLSDLHLRVNLSRLK
                     DRYGVEVGARPPRIAYRETVSGKADGHHRHKKQTGGAGQFGEVFLRIEPLPRGAGFEF
                     VDEVKGGTIPGQFLPAVEKGVRQVLSGGAVAGYPMQDVRVIVYDGKHHPVDSKEVAFV
                     AAGKKAFLDAIGKARPQVLEPIAELEVTAPEQHMGDISGGLSTKRARISGTDSARGGE
                     IVVKAQVPMSELEGYAAELKSITAGRGRYALDFSHYEPVPAQVQQKLVDAYKPHHEED
                     "
     gene            8874..9245
                     /locus_tag="PK818_01430"
     CDS             8874..9245
                     /locus_tag="PK818_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQY81428.1"
                     /translation="MANALQTSVVLYALLAGIAQDPPTPTGAGDPVAPLATIAWEATE
                     RPPMRGIIDALRRTRLDGLSTRIKLDFDADGKVVDAVLLESTGNQNLDAAILKWARDV
                     KLKPGSPGGPGVLPMTLLRPR"
     gene            9314..9865
                     /locus_tag="PK818_01435"
     CDS             9314..9865
                     /locus_tag="PK818_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81429.1"
                     /translation="MRRLATIIVLACAGLLAACSSLGSSVLLGRDISFTAAQLQEQLD
                     RKFPRDYRKLGGLVSISLLNPDLSLPGGGRLKLDFDLGIGAMGKPSRSPSGHFALESG
                     LRFDPGTRGLHLDNPEIVSVDVPALGGVMNDTARSLLNSWLLDYAREEPVYRLDDDTF
                     GRIAARRIDRVAIESGLVTLHLD"
     gene            9868..10248
                     /locus_tag="PK818_01440"
     CDS             9868..10248
                     /locus_tag="PK818_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81430.1"
                     /translation="MHAHRIGLLLCCTLLAACAGDKPDGAGAPGEDGLPKPAAASGSV
                     TGMPDPGVAGPRREARALPEPEPVELPQPIDPGESAPVDPAMASEGMPAMPSEPGQAP
                     EAAVPPVAPAADAAGTTDPASPRQ"
     gene            10245..10967
                     /locus_tag="PK818_01445"
     CDS             10245..10967
                     /locus_tag="PK818_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81431.1"
                     /translation="MKRLLPAACAVAIASACTPQPAPPAAPPGPAAAPAAIPPTAPIA
                     ADAAPARPQAAAESWVLPGSLGPLTTRLELDARFGKDNVREASFPGAEGIGTHPALVV
                     FATDPRKRLELVLDADNPDAPIRELRVSHPQSRWQLASGLRPGISLAELVALNGAPVS
                     FYGLGWDYGGTVQDWHGGKLANAVGNPLFHRVTLSARPDAGDATLPTGDTSVRSDDRR
                     WPSLGQDLVVAELGISWPDDGD"
     gene            complement(11050..11514)
                     /locus_tag="PK818_01450"
     CDS             complement(11050..11514)
                     /locus_tag="PK818_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HQY81432.1"
                     /translation="MPKKITTRRSAIHGNGMFAVAQIRKGERLIEYKGLRRTHEEVDA
                     GDSGDIESGHTFLFTLNEVFVIDANVGGNSARWINHSCAPNCEAVLVEHEDDPEKSQV
                     FIEAIRAIRPGEELTYDYGITLAERHTPRLKKIWACRCGAKDCTGTMLRPKR"
     gene            11707..12639
                     /locus_tag="PK818_01455"
     CDS             11707..12639
                     /locus_tag="PK818_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY81433.1"
                     /translation="MRLHPAALVALVAFVVLALLALPGNGQDRRNRMQQLREASRQER
                     AVAVPAGGVVLRNLAYGPDPAQRFDLYLPARATAAPVVFYVHGGGWANGDKTNRGLAN
                     KLAHWLPKGYAVISTNYRMLPVAMPLEQARDVARAVAQAQRRAGEWKLDPARVVLMGH
                     SAGAHLVALLGADPRLLAEAGASPPLGVVALDSGALDVPALMARPRVPQLYRDAFGAD
                     PAYWRSVSPQQQLGRSGLPMLLVCASERRFPTAPCDEARKFARKAGALHVPMSVQPEP
                     LDHGAINHDLGLDSGYTRKVTAWIDRLQAVSATR"
     gene            12710..13159
                     /locus_tag="PK818_01460"
     CDS             12710..13159
                     /locus_tag="PK818_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HQY81434.1"
                     /translation="MLRVTKLTDYASVVLTVLASDATRVLSASELAERAGLEAPTVAK
                     VLKPLAQAGLVEGFRGANGGYRLARDAAAISLVEIVEAMEGPLGMTECSVAASQCGIE
                     HSCGVRANWRRINDVVADALRGVSLAQMLQPAAAPPRKRIDMKLAKA"
     gene            13169..14620
                     /gene="sufB"
                     /locus_tag="PK818_01465"
     CDS             13169..14620
                     /gene="sufB"
                     /locus_tag="PK818_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HQY81435.1"
                     /translation="MNQSNEQNAQIHAQLGRKYEAGFVTDIESDSLPPGLSEDIIRAL
                     SAIKDEPEWMTEWRLAAYRHFLGMPVPDWAKLRIGPIDLQALSYYSAPKGPKYKSLDE
                     VPQELLDTYDKLGVPLHERAKLAGVAVDAVFDSVSVGTTYRKELAEKGVLFCSMSEAV
                     REHPDIVRKYLGSVVPTGDNYFAALNSAVFSDGSFVFIPKGVRCPMELSTYFRINAGH
                     TGQFERTLIVCEEGAYVSYLEGCTAPMRDENQLHAAVVELVVLDDGEIKYSTVQNWYP
                     GDENGVGGIYNFVTKRAECRGARSKVTWTQVETGSAITWKYPSCVLLGDDSVGEFHSV
                     ALTHHRQQADTGTKMIHVGKRTKSKIVSKGISAGHGQNSYRGLVKVASGADGARNHTQ
                     CDSLLIGKQCGAHTFPYIEVKNPTATVEHEATTSKISDDQLFYCRSRGIGQEDAVSLI
                     VDGFCKSVFRELPMEFAVEAKKLLDVSLEGSVG"
     gene            14678..15442
                     /gene="sufC"
                     /locus_tag="PK818_01470"
     CDS             14678..15442
                     /gene="sufC"
                     /locus_tag="PK818_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HQY81436.1"
                     /translation="MLKIDNLHASVAGKDILKGLSLQVNPGEVHAIMGPNGAGKSTLG
                     NILAGREGYEVTAGTVEFEGEPLLGLAPEERAAAGVFLAFQYPVEIPGVNNTYFLRAA
                     LNAQRKARGEPELDSMQFLKRVREKLAVLHLKDELLHRGVNEGFSGGEKKRNEIFQLA
                     LLEPKLAILDETDSGLDIDALKAVADGVNAQRSPGRAFLVITHYQRLLDYIRPDFVHV
                     LAEGRIVQSGGPELALELEAHGYAWLRDRVAPEAAA"
     gene            15444..16709
                     /gene="sufD"
                     /locus_tag="PK818_01475"
     CDS             15444..16709
                     /gene="sufD"
                     /locus_tag="PK818_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HQY81437.1"
                     /translation="MGALLDSLAAPFDGDAAQRAALDALLQDGLPHARVEAWKYTPLR
                     ALERRSFAAAGSATVTVDAELLAAIPAPRIVFVNGRFDPTLSALEGLPAGASLRPLSQ
                     LLAAADAHAAGLLQHRYQRRDEPFARLNEVLAREGAVLRIGDGVQVAMPLQLVFVGAP
                     AGGDQAWHLRQCIALGEGAGATVVEHHLGGGEHAHLGNAITHVEVAAGARLRHVRIQD
                     EASRATLFARTDAVLATAADYRRIDLELGAALTRHELNVRLEGEGANLQADGVLLADG
                     RRHVDTRLGIAHTARDTSCSLTWRGLAGQRGRAVFHGGISIAAGADGSEAALSNKNLL
                     LSAEAEIDSQPVLEIHADEVKAAHGATVGQLDPTALFYLRSRGLPEADARRLLTAGFC
                     REIVAGVGDTGLRAQLGAALDRALARVDA"
     gene            16760..17980
                     /locus_tag="PK818_01480"
     CDS             16760..17980
                     /locus_tag="PK818_01480"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006448899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HQY81438.1"
                     /translation="MRAVDWDAVRADFPLLARHVHGKPLVYLDSANTGQKPAAVIEAV
                     DGFYRRHNANVSRAVHALGAEATDAYEGARRKLAGFLNVRHDDLVLCSGTTFAINLVA
                     YAWALPRLQPGDAILLTRMEHHANIVPWQLVAQRTGATIKVAELREDGSLDLDALFAA
                     MTAEVKLLGFTHVSNVLGTINPVAAICREARRRGIVTVVDGSQAAPHMALDLPALGCD
                     FYAITGHKMCGPTGTGALWARREHLQAMPPFLGGGEMIREVRFEGTVYNDPPHRFEAG
                     TPNIAGHVGLGAAVDYLSALGMHNVAAREAELLAHATEELRKLDGLRIIGEASEKAAV
                     ISFLVDGAHAHDLATLLDLEGVAVRSGHHCAHPLMQSFGVPATCRASFAFYNTHAEVE
                     AFIAALRKVRGLLA"
     gene            17977..18525
                     /locus_tag="PK818_01485"
     CDS             17977..18525
                     /locus_tag="PK818_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY81439.1"
                     /translation="MSALAYRAATAADIDAVVALVESAYRGDASRQGWTTEADFLDGR
                     RTGADDVAACIERERSRILLAERDGVMLACAHVAEGQTEQERGAGYFGMFSVRPGLQG
                     GGIGKQVLAEAERIAREDWGLAVMRMTVIDIRAELIAFYERRGYHRTGIRKPFPYGDE
                     RFGLPLRDDLRFEVLEKELADG"
     gene            18518..18841
                     /locus_tag="PK818_01490"
     CDS             18518..18841
                     /locus_tag="PK818_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014508448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HQY81440.1"
                     /translation="MGDWVFVCTTAQLLPGEMTVAWDGDTAIVVFNHDGSFHALEDRC
                     SHEDYELSTGSFDPATGSIECVLHGARFDVHDGRALCAPAYEPATPFPVRVDEAGIWT
                     RDDRK"
     gene            18940..21096
                     /locus_tag="PK818_01495"
     CDS             18940..21096
                     /locus_tag="PK818_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HQY81441.1"
                     /translation="MPFQPASRRARLLSRSPLALALLTLVAPLAQAAGTDPATDPAEP
                     TDLDKVQVVGQRYLPDYAARATRSATRTDTPLLDVPQAITVVTDKLISDQAMTSLSDA
                     FRYMPGIGLAQGEGNRDTPVLRGNSSTADFFVDGIRDDVQYVRDTYNVERIEALKGPN
                     AMVFGRGGSGGVINRVTRQADGQSHRAGSLQVGSGNRRRGTVDVGDAIGEGSAGFRIT
                     AMYEDSDSFRDGYTLERHGINPTFGVDLGERTRLQLSYERFHDERVADRGVPSLYGRP
                     VETDPSTFFGNPDASPVEATVDALDAVIEHAFADNLSLRNHLRWADYDKFYQNVFPGA
                     ADAATQTVSISAYNNSTTRENLFNQTDLVWETATGGIRHTVLAGAEFGRQVTDNLRMT
                     GYFGAPGSTTTSIRVPLSSPTTSIVPLFRQSATDADNHGVARVAAVYLQDQVEFTPQW
                     QAIIGLRHDDFRVELRNNRTGAILRSDDGLLSPRAGLVYKPVERVSLYASYSMAYQPR
                     AGEQLASLSASNAALDPETFRNRELGAKWDIRPELQASIAVYRLDRGNVAVTDPADAT
                     RMILVDGQTTEGVEIGVAGRITENWQLMGGYAYQDGEITTTQSATAVAGNRLAQLPEH
                     SASLWNRYDFNQAFGIGLGIIHRGSIFANVDNTVTLPSFTRFDAALYWTLNPAMQLQL
                     NVENLGNKRYFASAHSNNNISPGTPRSAWVSLNFHF"
     gene            21111..21752
                     /locus_tag="PK818_01500"
     CDS             21111..21752
                     /locus_tag="PK818_01500"
                     /inference="COORDINATES: protein motif:HMM:NF027675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HQY81442.1"
                     /translation="MTMDANKPGRRGPAATYRWIRQLHLWVGAWGALAAILYGITGLV
                     MNHRFGDGAWPQGDSIEAGRTALAIPAQARTSPEQLSLWLRDARQLDAQVIRKGGPGG
                     RGGDARQPPKWNLSGGTARNAWSLEYAPGSETAELKQTSHTTLAALNRLHKGVGGGGW
                     WILLADSFAIGMLLLGLSGIWMWARGRSAREMLASVMGLSTLAFVAVVGLALL"
     gene            complement(21749..22369)
                     /locus_tag="PK818_01505"
     CDS             complement(21749..22369)
                     /locus_tag="PK818_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HQY81443.1"
                     /translation="MGSPSPQSSQVLRAGGYRRMRWKNDAGWTSEILKVHDGDERDTN
                     DWAWRLSIAEIESDAPFSAFPGVERVLVLLSGNGLRLRFDDGESHALRPPHDQLRFAG
                     ERAAFGELIDGPTRDFNLMWKRDLADAALWHRPLVGSMVVFVEPAETWAVHVLAGQAR
                     FADESGLGGVQMGDTAILVAADARTRYVLDGGGEVLLVRVRPVDPA"
     gene            complement(22401..22742)
                     /locus_tag="PK818_01510"
     CDS             complement(22401..22742)
                     /locus_tag="PK818_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006452365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQY81444.1"
                     /translation="MKSLTTLFSSLILSLALAGAAIAGETVNINTADAATIDRVLVNV
                     GPAKAESIVAYRKANGAFRSAEQLALVKGIGLKTVEKNRDRIALGAARPAAKAGTKVA
                     AKPMPATVARR"
     gene            22932..24425
                     /locus_tag="PK818_01515"
     CDS             22932..24425
                     /locus_tag="PK818_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011407897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HQY81445.1"
                     /translation="MDASRTKAFVDAMWDEQIVPQLVEYIRIPNKSPMFDADWVKNGH
                     MDRAVALMQAWAKAQPVPGMQVEVVHLQGRTPLIFLDIPATDGGGGDDCVLLYGHLDK
                     QPEMTGWDEGKGPWVPVLEGERLYGRGGADDGYAIYGSLTAIMALQAQGVAHARCVVL
                     IEACEESGSYDLPAYVDHLAERIGKPSLVVCLDSGCGNYDQLWCTTSLRGLAGGNLTV
                     KVLDEGVHSGDASGIVPSSFRIIRQLLSRIEDEASGRILVEGLHVDIPAERKQQAAKV
                     AEVLGTEVYDKFPFLPGMHPMAEDLTELVLNRTWRPALSITGADGIPPLASAGNVLRP
                     STAVKLSLRLPPTLDGKRGGELLQQILLRDPPSGAHVSLHLEKASTGWNAPALSPWLA
                     QAIDQASAEFFGKPAMYMGEGGTIPFMGMLGEKFPGAQFMITGVLGPHSNAHGPNEFL
                     DIPMGKGVTACVARVIAEHHAARLRGETSGVAAMAGNSYADTPGGCC"
     gene            24640..>25370
                     /locus_tag="PK818_01520"
     CDS             24640..>25370
                     /locus_tag="PK818_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HQY81446.1"
                     /translation="MSPAALPLPSPLSSEHGALLAQFAARAETASLLWASGYLAGLAR
                     ALDAPAQPQVPAHPDAGVRTTVLYGSQTGNARRAAEALHARLEAAGLPARLLRADAYP
                     LRELKDERLLYIAISTQGDGEPPDDALAFVEFLSGKRAPRLPQLKFAVLALGDSSYPQ
                     FCAIGQRLDARLSELGAVRVAACANADLDVETIAAPWTETVVGLAQELLKRAPPQAAA
                     AHATVVPLRPHAVPQAHGRERPFAA"
BASE COUNT         3980 a         9031 c         8752 g         3607 t
ORIGIN      
        1 gattcgcggc cctcgcccac gcattcgaac ggcttgtggt cctggtattc cagcagggcg
       61 tcgaagcccg cggtctgcgc ggggtcgtcc agcaggttgc ggccgatgat gcccaccagc
      121 cgcggcttcg gcatgaatgg cgccagtgcc aggaacacga agtggcactt cgggcagacc
      181 ccgcaccagc ggctggtcgg gcgctcgccg aggatgtgga agttgcggtt gcagctggag
      241 aaatgcgcgt cgtagcggct gacccgcgcg aactgccggg ccaccgccag ctcgctcagc
      301 ggccgcagca acgagtagta gcgcaggtcg gcggcgacgt gccgctgcag ccaatcgccg
      361 agcgcggatt cgaacgccca gcccttcgac cactggtggt tcacctcgcc ggtgacgctg
      421 ccgtcatcag caatgatgat cgacccgtag ctggccgaac gctcgttgga gaacaccacc
      481 tggtccacgc ccagcagcac cgcggcgaag gccaggatcg ccgagttcac cgcggtcacc
      541 gggatgtggc cgttccatgc gcccagccgg ttgtattcga acagctgcga agcaagctgg
      601 cggccgatgt tcagcgtggg caggccggta tgcgcggcgc aggccgcgat caattgcgag
      661 ccgccgatcc aggtcacggt ctgttcgacg ccggcaccac gcagcgcctc gatgctgacc
      721 agcgaatcct tgccgccgcc gatcgcgacc agcgcgtgtc cgcgcaggcc cagtgccggc
      781 gcaggcaccc ctgcctgcgc atctgccggg aagcggatcc ggccgcgcag gtccagcccg
      841 ttgcgatagg cgaactcacc gaggccgttg aggtagacgg tttcgaggaa cgccgcggta
      901 tcggcatcga tctcgtacgc ctcgatgcgg atatccgtcg gcaccgcggc cttgtagtag
      961 ctgacgccgg cgaccaggtg cagcagccgc agcgcctgcc ccgccgccgc cgcgcgcgca
     1021 tcgtcgaggt cgaacggcgc gcccggcacg gtgaccgtct ccaccagttc cgggccgtca
     1081 tcgaaggcat acaccagccg cgcgatgccg gtctgcgcat cgaaggcgca gcgcacgaag
     1141 cggaacgtgc ggatcgcgtc gcgctcgaac atccactcac tcattcaggc ataccttctt
     1201 caagcaggtc ctccgcgggc agcgcgcgca ggttgtattc gttggacatg ctgcggccgt
     1261 aggcgccggc atcggccacc agcatcgcgt ctccgggcgc ggtcccggca ggcagcttga
     1321 cccgctggcc gaacacgtcg ctggattcgc agatcgggcc gaccacgtcg aaatcgatgt
     1381 cgccaccggc atccagccgc gacaggttga cgatgtcgtg ccacgcgtcg tacagcgccg
     1441 gccgcaagag tgcgttcatg cctgcatcca ggccgacccg gcgcacgccg tccttctcga
     1501 tcacctgggt cgcgtgcgcc agcaacacgc cggcttcggc gaccaggaag cggccgggtt
     1561 cgatcgccag cctgaacgcc ggatgcaggg ccttgatcgc ggccaggcct tcggcccagg
     1621 catcgaggtc gaagggttcg tcgtcgtcgc tgtaggcgat cggcaggccg ccaccgatgt
     1681 cgatggtctc cacgctgccg atgcgccgcg cgaagcccgc cagttcatcg accacgccct
     1741 tccagtggtc gacggtttcg atgccgctgc ccaggtgcgc gtgcacgccg gtgatgcgca
     1801 cgtccagcgc gcgcgcggct tcgacgaatt cctcgacgcg ctgcgcggac aggccgaact
     1861 tggagtcctt gccgccggtg ttgaccttgg cgtggtggcc atcgccgtgg ccgaggtcga
     1921 tgcgcagcca cagcgcgcgg ccgcggaaca cccccggcca gcgccgcagc agctccacgt
     1981 tgtccacggt gacggtgacg ccacgcgcca gcgcggcttc gtattcgccg atcggggcga
     2041 agctgggggt gaacagcacg cgcgccggat cgaagccggg cacggcctcg aacacgcgtt
     2101 ccacctcgcc ttgcgacacg cattccaggc cgaagccttc cgcggcaagc gcgcgcagca
     2161 gcgccggatg cccattcgcc ttgatcgcgt agtagcggcg gtcgatcgca tcgatgccct
     2221 gcaactggcg cgcgcgcgcg cgcaccgtcg gcaggtggta gacatagcgc ggcgtgccgt
     2281 ggccggcgag ttccagcagg cgttcgcgtt cgcgcggcgc gcgccaccac gcgggcgggc
     2341 gcgggcgcac cgtgccattg atctcgcgcc agcgcgggcc gaacacctcg gcctcgtaga
     2401 ccggcatcgc gccgctgtcg atcagctccg catgcagccg gtgcagcagg ccgtcggccg
     2461 cggcctcgtc gaccacgaag gtcaggttga ggtcgttcga cgactgcgag atcaggtgca
     2521 cccgctcgcg cccgaacatc tcccacaccg cggacagctt gtgcagcagc gagcgcatgc
     2581 cgcgcccgac cagggtcacc gccgcgcacg gcacgatcac cttgacccgg cacacctgcg
     2641 ccaggtcctc cgacaaacgc gcaagcacgt cggtgctcac caggttctcc gagggatcca
     2701 gcgacacggt gacgttggtc tcggacgagc cgatcaggtc caccgacagg ccatggcgct
     2761 tgaagcgctc gaacacgtcg gccaggaagc cgacctgctg ccacatgccg acgctctcca
     2821 tcgacaccag cacgatgccg ttgcggcggc tgatcgcctt gaccccgggc accgcggcca
     2881 cgctgccctc gatccgggtg cccggcagtt gcgggcggct ggtgtcgagg atgcgcatcg
     2941 gcacgccgct gtcgcggcag ggcttgatcg agcgcgggtg caggaccttg gcgccggtgg
     3001 tggcgatttc ctgcgcctcg gcgtagtcca gccgggtcag caggcgcgca tccggcacgt
     3061 cgcgcgggtt ggcgctgaac atgccgggca cgtcggtcca gatctcgacg cggcgggcgc
     3121 cgagcagcgc accgaagtac gcggccgaag tatccgaacc gccgcggccg aggatcgcgg
     3181 tgccgccatc gggatggcgg gcgatgaagc cctgggtcag cagcagttgc gccggctgcg
     3241 cggcgaaacg cacgcgcagg gcggcatccg catcgaagcg gcagttgacc gacagccgct
     3301 gcgcccagtc gctggcattg ggcagcgaga ccgcatccag ccagtcgcgc gcgtcgcacc
     3361 agccgaaatc caggccctgc cggcgcaggt aggcggcacc gatcgaggac gacagcaact
     3421 cgccctgcgc cagcacctcg gcctgccagt ccagcgaccg cgatgccgcg cgcggatcgt
     3481 cgccgagggc gtgcaatgcg cgcaggcgtt cgccgatggc ggtgtcggga tcgatgccga
     3541 gttcgtcgct gcagaacgcg cgatggcggg cgaccagcgc ggcgatgcgg cccttgacgt
     3601 catcgccgtt gctgatcgcc tgcagttcgt tggtgacgcc ggacagcgcc gacaccacca
     3661 ccagcacgcg cgcacccccg gccgccgcgc gtccctcggc cagctggccg atggtgtccc
     3721 agcgctcgcg tttggacacc gaggtcccgc cgaacttcag caccagccag ggctgtgcag
     3781 gggcggtggg atcggacgcg gcggcgatgg acataggccc tttcggaatc agtcggggga
     3841 tgcccgcggc acgggcagtg gtgcattcta gcgggccttt ggatccgtgc cccttgcccg
     3901 ccatgccccg tcgttatgtg caactcgatg tgttcgcgtc gcgccccggc gccggcaacc
     3961 cgctcgccgc cgtgctcgat gcggagggca tggacaccgc ctcgatgcag gcactggcgg
     4021 cgtggctcaa cctgtcggaa acgatctact tcctgccacc cgatgccggc gccgattacc
     4081 ggatccgcat cttcaccccc ggctgcgaac tgccattcgc cggccacccc agcgtgggcg
     4141 cggcctggct ggcggccacc cacgggctgg ccacgccacg cgatggccag ctggtccagc
     4201 aatgcgcggc cggcctgctg ccggcgcagg tgcacggcga tccgctgcgg ccggggatcc
     4261 tgctgcgcag tccgcgcggg cgcctgctcg aggcggccac gggcgcgctg ccgcgggaac
     4321 tggccgcgat cgcacggccc gggcaagccg tcgaactgtg gagcaacggt ccgcgctggt
     4381 ggctgctgga agccgggtcg gccgacgcgt tgcgcgggct gaagccggat ttcggcgcga
     4441 tcgccggctg gaccgatgcc accgggtcga ccggggtcgc ggcattcgcc ttcgaagacg
     4501 gtggcgacca ccagctggtg gtgcgcgcgt tctgccccgg tgatgccgtt ggagtgcccg
     4561 aggacccggt caccggcagc gccaatgcgg tgatcgcggc gcgcttgcat cgcgaaggcc
     4621 ggctgcccgg ccgcgacggg cgctacatcg ccagccaggg ccgcgagctc ggccgcgacg
     4681 gcagggtcac gctgcaggtc gatgccgatg gcgaggtctg gatcggcggc gaggtgcaac
     4741 aggtcatagc cggcagcctg gaatggccgg atgcgcaagc caccgggcct gcaagcccat
     4801 gacccggcgc cgctacgtgc aactcgatgt gttcgcctcg cgcgccggcg acggcaatcc
     4861 gctggcggtg gtgctggacg ccgaaggcct ggacgacgcg cgcatgcagg cgatcgcccg
     4921 ctggacgcgg ctgccggaaa ccaccttcgt gctgccgccg gtggccaccg acgccaccca
     4981 ccgcatccgc atcttcagcc cgcgccggga agtcccgttc gccggccatc ccagcgtcgg
     5041 caccgcgcat gccttgctgg atgcgggcct ggccgcgccg cgcgacggac tgctggtgca
     5101 ggacgggatt gccggcaagc tgccgctgat ggtcaccggc gcgggccgcg aacgcacgat
     5161 cgccgtgcgc acgccacgcg cacgcgtgca ggaaatcgca acgcccgacg acccgcgcct
     5221 gcgcgatgca cttgccggat tgccgctggg cggactgccg ccggtgctga tggacggcgg
     5281 ccgccgctgg tggctggcgg agctggccag cgaggccgac cttcgcaacg cacagccgaa
     5341 ctgggaggcg atcgtcgcgc tcgcgcacgc gaccgacagc atgggcctgt tcgtctatgc
     5401 acgcagccag gatccggtcc actacttcgc ggtgcgcgca ttcgtcggtg caccggcacg
     5461 cttcgaggac gccgcctccg gtgccgccaa cgccaccctg gcggcgtggc tggccgaatg
     5521 cggtgccctg cccgatggcg gcttctaccg ggtcagccag ggccgcgaag tcggccacga
     5581 cgcgatcatc gaactccgcg tggatgccgg cggcgacgtc tggtcgggcg ggcgggccgt
     5641 cgccgtggtc accggcaccc tggattggcc cgactagcga tgcgccgggc gcggccgccg
     5701 cacccctggt cggcatcgag gcaacacaaa tgaagaaggg gccgcgcggc cccttcgcct
     5761 tgatcagggt gacgcctgtt actgcggcga cacatccacc cgcacgcgca cgcggtcgcc
     5821 cgggtggtag tccatccggc gggtgtagcg gcggccgttg tattcgtaag ccacgtcata
     5881 ggcgctggcg cgtccatcgc cggatccgct gtagccgctg ccgtaaccgc cgcccgccgg
     5941 ttccgggtcg cacacccgca ccacgccggc gcgcacgtag cggtcgcgct gggtctggtc
     6001 gtagatcccg cggcctgcca tgccacccac catcgagccg atcgcggtgg cggcataggt
     6061 gccgctgccg ccaccgacct tgctgcccag caccgcgccg gcgatgccgc cgaccacggt
     6121 ggcgatgttg cggcccgcca tgctgccgcc ctgggtgcgg ccgtacgggt cgtagtagcc
     6181 gccattgcga tagccatcat ttcggtagcc atcgacgtcg tcgtagccgt cgcgctggtc
     6241 gttgccggcg ctggtggttt cgtagcaacg ctggccgacg gcgctggacc caccgccgta
     6301 gcgaccatcg agcaccgggt cgacccggat cacgcgcgca tagtcgtagt aggcctcgcc
     6361 ctgctgctgg ccgttgaagc catcgccgga cggatacggg tacgactgcg cggacgcggt
     6421 gccgccggag gcaaggccga cggcaacaag caaggcaacg gcaaggcgct tcatggtgga
     6481 ctccggtcgc cgggatgggt ggagggccgg cgcgtcgcga acactgcggc cacgccgcta
     6541 acgcgaagct gaatccggtg gcttcgcccg aatccgttta gcgcggcgac agtttgcggg
     6601 cgcagcccgc ggccgccatc gcggttccgc caccggcagc gaagcgcgcc gcgcatgctg
     6661 caccgcaaga caagacccgg ttccgcgctg cgaggttccg cgctaaggtg gatgcccgcc
     6721 atgaccgccc gccgagcgcc cacgctccgg cgcgaagcgc ggacagccat gacgctcact
     6781 ccacgcactc gaggagaaac gggctcatgt cctacagcac acagcagatc cgcaacgtgg
     6841 ccctcgcagg gcaccctggc gccggcaaga caaccctgtt cgaagccctg ctgcatgccg
     6901 gtggcgcgat ccaggcggca ggcacgatcg aacgcggcag caccgtctcc gacttcgacc
     6961 cgatcgagaa ggaacgcggc cactccatcg attctgcgat cgccagcacc gaccacgcgg
     7021 gcatccacct caacctgatc gacacccccg gctatccgga tttccgcggc cccaccctgt
     7081 ccgcgctggc ggcggtggag acggtggcaa tcgtggtcga tgccgacacc ggcgtcagct
     7141 acggcaccca gcggatgatg gagcacgcca aggcgcgcaa cctgtgccgg gtgatcgtgg
     7201 tcaacaagat cgaccacccc ggcgcgaagc tgggggcatt gctcgacagc ctgcgcgaga
     7261 ccttcggcgc cgaggtgctg ccgctcaacc ttcccgcgga gggcggcaag tcggtggtca
     7321 actgcttctg gcaatccacc gggaacagca acctcggcgc ggttgccgac tggcaccaga
     7381 agatcatcga ccaggtggtg gagatcaacg aaacggtgat ggaccgctac ctcgacgtcg
     7441 gcgagggcgg cctgtccggg caggaactgc acgacgcctt cgagcaatgc ctgcgcgaag
     7501 gccacctggt gccggtgtgc ttcgtgtcgg cccgcagcgg cgccggcgtg cgcgaattgc
     7561 tggacgtcgc cgagaagctg ttcccgcacc ccggcgaagc caacccgccg ccgttcgtga
     7621 agggcaccgg cgacgacgcg caggcgatca ccgccacccc ggacccgcag gcgcacgtga
     7681 tcgccgacgt gttcaagatc atcaacgatc cgttcgtcgg caagctcggg atcttccggg
     7741 tctaccaggg cacggtgaag aaggacaccc agttgttcgt cgacgacggc aagaagccgt
     7801 tcaaggtcgc gcacctgttc aagctcaagg gcaaggacca cgtcgaggtc gtgcaggcga
     7861 tcccgggcga catcgccgcg gtggccaaga tcgacgaggt gcacttcgac gcggtgctgc
     7921 acgacagcca cgacgaggac cagatccacc tggcgccgct ggcgttcccg cggccgatgt
     7981 tcgggctggc gatcgaggtg gcgcgcaagg gccaggagca gaagctcgcc aacgccctgc
     8041 accacctcgc cgaggaagac ccctgcttcc acgtggagca cgagctggag accaacgaaa
     8101 ccgtggtccg tggcctctcc gacctgcacc tgcgggtgaa cctgagccgg ctcaaggatc
     8161 gctacggggt cgaggtcggc gcgcgtccgc cgcggatcgc ctatcgcgaa accgtcagcg
     8221 gcaaggccga cggccaccac cgccacaaga agcagaccgg cggcgccggc cagttcggcg
     8281 aggtgttcct gcggatcgaa ccactgccgc gcggcgccgg cttcgagttc gtcgacgagg
     8341 tcaagggcgg caccatcccc ggccagttcc tgccggcggt ggagaaaggc gtgcgccagg
     8401 tgctgtccgg cggcgcggtc gccggctatc cgatgcagga cgtgcgggtg atcgtgtacg
     8461 acggcaagca ccatccggtg gattccaagg aagtcgcgtt cgtggccgcc ggcaagaagg
     8521 cgttcctgga tgcgatcggc aaggccaggc cgcaggtgct ggagccgatc gccgagctcg
     8581 aggtcaccgc ccccgagcag cacatgggcg acatcagcgg cggcctgtcg accaagcgcg
     8641 cgcgcatcag cggcaccgat tccgcccgcg gcggcgagat cgtggtcaag gcgcaggtgc
     8701 cgatgtcgga gctggagggc tacgccgcgg aactgaagtc gatcaccgcc ggccgtggcc
     8761 gctacgcgct ggatttcagc cactacgagc cggtaccggc gcaagtgcag cagaagctgg
     8821 tcgacgcgta caagccgcac cacgaagaag actgagcccg ggcaggagcg gtcatggcca
     8881 atgcattgca gacgtcggtc gtgctgtatg cgctgctggc gggcatcgcg caggatccgc
     8941 ccacgccgac cggcgcgggc gatccggtcg cgccgctggc gaccatcgcc tgggaagcga
     9001 cggagcggcc gcccatgcgc ggcatcatcg acgccttgag gcggacccgg ctggatggcc
     9061 tgtccacgcg gatcaagctg gacttcgatg cggacggaaa ggtggtcgat gccgtgctgc
     9121 tggaaagcac gggcaaccag aacctggatg cggcgatcct gaaatgggcc agggatgtga
     9181 agctgaagcc ggggtcgccc ggcggacccg gcgtgctgcc gatgaccctg ctgcgccctc
     9241 gctgagggcg ggctggcgcc gccatccgtc gcaacccgtt aatgtgggcg cagctcccca
     9301 ccggacccct gccatgcgcc gccttgccac gatcatcgtc ctcgcctgcg caggcctgct
     9361 cgccgcctgc tccagcctcg gttcctcggt cctgctcggc cgcgacatca gcttcaccgc
     9421 agcgcaactg caggagcagc tggaccgcaa gttcccgcgc gactaccgca agctcggcgg
     9481 gctggtcagc atcagcctgc tgaatccgga cctgagcctg ccgggcggcg gccgcctgaa
     9541 actggatttc gacctcggca tcggcgcgat gggcaagccc agccgcagcc cgtcgggcca
     9601 cttcgcgctg gaaagcgggc tgcgcttcga tcccggcacc cgcggcctgc acctggacaa
     9661 tccggaaatc gtcagcgtcg acgtgcccgc gctcggcggc gtgatgaacg acaccgcgcg
     9721 ctcgctgctg aacagctggc tgctggacta cgcccgcgag gaaccggtct accgcctcga
     9781 cgacgacacc ttcggccgca tcgccgcgcg ccggatcgat cgcgtcgcga tcgagtccgg
     9841 gctggtcacc ctgcacctgg attgaacatg cacgcccatc gcatcggatt gctgctgtgc
     9901 tgcaccctgc tggccgcctg tgctggcgac aagcccgacg gcgcgggcgc gccgggcgag
     9961 gacggcctgc cgaaacccgc cgccgcaagc ggctcggtga ccggcatgcc ggaccccggg
    10021 gtcgccggcc cgcggcggga ggcccgcgcg ctgccggagc cggagccggt cgagttgccg
    10081 cagccgatcg atcccggcga atccgcgccg gtcgatccgg cgatggcgtc cgagggcatg
    10141 ccggccatgc cgtccgaacc cggccaggcg ccggaggcag ccgtgccgcc ggttgcgcct
    10201 gccgcggacg ccgcgggcac caccgacccg gccagcccgc ggcaatgaag cgcttgctgc
    10261 cggccgcctg cgcggtagcg atcgccagtg catgcacgcc gcaaccggcg ccacccgccg
    10321 ccccgcccgg gccagcggcg gcgccggccg cgataccgcc aaccgcgccc atcgccgcgg
    10381 acgcggcgcc ggcacgcccg caggccgccg ccgaatcctg ggtcctgccc ggcagcctgg
    10441 gaccactgac cacgcggctg gaactggacg cgcgcttcgg caaggacaac gtgcgcgagg
    10501 cgtctttccc gggcgcggag ggcatcggca cccatccggc gctggtggta ttcgccacag
    10561 acccgcgcaa gcgcctggag ctggtgctgg atgcggacaa cccggatgcg ccgatccgcg
    10621 agctgcgggt gtcgcatcca cagtcgcgct ggcagctcgc ctccggcctg cgcccgggca
    10681 tcagcctggc cgaactggtg gcgctgaatg gcgcgccggt cagcttctac ggactcggct
    10741 gggactacgg cggcaccgtg caggactggc acggcggcaa actggccaac gcggtcggca
    10801 acccgctgtt ccaccgggtc accctgtccg cgcgcccgga cgcgggcgat gccaccttgc
    10861 cgaccggcga caccagcgtc cgcagcgatg atcgcaggtg gccgtcgctg ggccaggacc
    10921 tggtggtcgc ggaactcggg atcagctggc cggacgacgg cgactgaaac gcgggcgcac
    10981 ggacttgcac gccgcgcacg ccgggtcgtg gatgcggaat catcgcgagc cggatgacgc
    11041 gctggtacct cagcgcttcg gccgcagcat cgtcccggtg cagtccttcg cgccgcagcg
    11101 gcaagcccag atcttcttca gccgcggggt gtggcgctcg gccagggtga tgccgtagtc
    11161 gtaggtcagt tcctcgcccg gcctgatcgc acggatcgcc tcgatgaaca cctgcgactt
    11221 ctcggggtcg tcttcatgct ccaccagcac cgcttcgcag ttcggcgcac agctgtggtt
    11281 gatccagcgc gcactgttgc cgcccacgtt ggcgtcgatc acgaacacct cgttgagcgt
    11341 gaacaggaag gtgtggccgc tctcgatgtc gccgctgtcg ccggcatcca cttcctcgtg
    11401 ggtgcggcgg agtcccttgt actcgatcag gcgctcgccc ttcctgatct gggcaacggc
    11461 gaacatgcca ttgccatgga tggcggaacg gcgggtggtg atcttcttcg gcatcggatc
    11521 ggccagggtg gaggagcgcc attgtggcgc aggcccgggg cgcgcgtcac cgcatgcccg
    11581 cgcaccgcca gtgactgtgc cggccgccac aacgcccgct gcaccggcat cgccggcgtg
    11641 cgcgcggtcg gtcaaccgcg cgcgggacgg cacgttggca gcagcagtcc ccgcggagat
    11701 tcccgcatgc gcctgcaccc cgcagcactc gtcgcactcg tcgccttcgt cgtactcgcc
    11761 ctgctggcgt tgcccggcaa cggccaggac cggcgcaacc gcatgcaaca gctgcgcgag
    11821 gccagccgcc aggagcgtgc ggtcgcggtg ccggccggcg gcgtggtgct gcgcaacctc
    11881 gcctacggac cggacccggc gcaacgcttc gacctgtacc tgccggcccg ggccacggcg
    11941 gcgccggtcg tgttctacgt gcacggcggc ggctgggcca acggcgacaa gaccaaccgg
    12001 ggcctggcca acaagctggc gcattggctg ccgaagggtt acgcggtgat ttccaccaac
    12061 taccggatgc tgccggtggc gatgccgctg gagcaggcgc gcgacgtcgc ccgcgcggtg
    12121 gcgcaggcgc agcggcgggc cggcgaatgg aagctcgatc cggcccgggt ggtgctgatg
    12181 ggccattccg cgggcgcgca cctggtggcc ctgctcggcg ccgacccgcg cctgctggcg
    12241 gaggccggcg caagcccgcc gctgggcgtg gtcgcgctgg acagtggcgc gctggacgtg
    12301 ccggcgctga tggcccggcc gcgggtgccg cagctgtacc gcgacgcctt cggcgccgat
    12361 cccgcgtatt ggcgttcggt ctcgccgcag cagcaactgg gcaggagcgg cctgccgatg
    12421 ctgctggtct gcgccagcga gcgccgcttc ccgaccgcgc cctgcgacga ggcccgcaag
    12481 ttcgcccgca aggccggcgc gctgcatgtg ccgatgagcg tgcagccgga gccgctggac
    12541 catggcgcca tcaaccatga cctcggcctg gactctggct acacccgcaa ggtcacggcc
    12601 tggatcgacc gcctgcaggc cgtttccgcc acccgctagg gcgccgcttg gctgcggccg
    12661 cccgaaatcg tacaattccg gtacgctttc aaggcacgcc ttcgcagcca tgctccgggt
    12721 caccaaactc accgattacg ccagcgtggt gctgaccgtg ctggccagcg atgccacccg
    12781 cgtgctcagc gccagcgagc tggcggagcg cgccgggctg gaagcgccga ccgtggccaa
    12841 ggtcctcaag ccgctggccc aggccgggct ggtggaaggc ttccgcggcg ccaacggcgg
    12901 ttaccggctc gcgcgcgacg ccgcggcgat ctcgctggtc gagatcgtgg aggcgatgga
    12961 aggcccgctg ggcatgaccg aatgcagcgt ggccgcaagc cagtgcggga tcgaacactc
    13021 ctgcggcgtg cgcgccaact ggcggcgcat caacgacgtc gtcgccgatg cgctgcgcgg
    13081 ggtctcgctg gcgcagatgc tgcaaccggc agccgccccg ccgcgtaagc gcatcgacat
    13141 gaaactggcg aaggcctgag aggtcgccat gaaccaatcc aacgaacaga acgcgcagat
    13201 ccacgcccaa ctgggccgca agtacgaggc cggcttcgtc accgacatcg aatccgattc
    13261 gttgccgccg ggactgtcgg aggacatcat ccgcgcgctg tcggcgatca aggacgagcc
    13321 ggagtggatg accgaatggc ggctggccgc ctaccggcac ttcctgggca tgcccgtgcc
    13381 cgactgggcg aagctgcgga tcggcccgat cgacctgcag gcgctgagct actacagcgc
    13441 gccgaagggc cccaagtaca agtcgctgga cgaagtgccg caggagctgc tggacaccta
    13501 cgacaagctg ggcgtgccgc tgcacgaacg cgccaagctg gccggggtgg cggtggacgc
    13561 ggtgttcgat tcggtcagcg tgggcaccac ctaccgcaag gaactggccg agaagggcgt
    13621 gctgttctgc tcgatgtcgg aagcggtgcg cgagcatccc gacatcgtgc gcaagtacct
    13681 cggcagcgtg gtgccgaccg gcgacaacta cttcgccgcg ctgaactcgg cggtgttctc
    13741 cgatggcagc ttcgtgttca tcccgaaggg cgtgcgttgc ccgatggaac tgagcaccta
    13801 cttccgcatc aacgccggcc acaccggcca gttcgagcgc accctgatcg tgtgcgagga
    13861 aggcgcctac gtgtcctacc tcgaaggctg caccgcgccg atgcgcgacg agaaccagct
    13921 gcacgcggcg gtggtggagc tggtggtgct ggacgatggc gagatcaagt acagcaccgt
    13981 gcagaactgg taccccggcg acgaaaacgg ggtcggcggc atctacaact tcgtcaccaa
    14041 gcgcgccgaa tgccgcggcg cgcgcagcaa ggtgacctgg acccaggtcg agaccggcag
    14101 cgcgatcacc tggaagtacc ccagctgcgt gctgctcggc gacgacagcg tgggcgagtt
    14161 ccacagcgtg gcgctcaccc accaccgcca gcaggccgac accggcacca agatgatcca
    14221 cgtcggcaag cgcaccaagt cgaagatcgt cagcaagggc atcagcgccg gccatggcca
    14281 gaacagctat cgcgggctgg tcaaggtcgc gtcgggcgcc gacggcgcgc gcaaccacac
    14341 ccagtgcgat tcgctgctga tcggcaagca gtgcggcgcg cacaccttcc cctacatcga
    14401 agtgaagaac ccgaccgcca ccgtcgaaca cgaggcgacc acctcgaaga tcagcgacga
    14461 ccagctgttc tattgccgtt cgcgcggcat cggccaggag gacgcggtga gcctgatcgt
    14521 ggacggcttc tgcaagtcgg tgttccgcga gctgccgatg gagttcgcgg tggaagcgaa
    14581 gaagctgctg gacgtgagcc tggaaggcag cgtcgggtaa ctctctcccg ccctttcccg
    14641 gcagcgggag gggaagaaaa cgaatacgga aaccaacatg ctgaagatcg acaacctcca
    14701 cgccagcgtc gccggcaagg acatcctgaa gggcctgtcg ctgcaggtga accccggcga
    14761 agtgcacgcg atcatgggcc cgaacggcgc ggggaaatcc accctgggca acatcctggc
    14821 cgggcgcgag ggctacgagg tcaccgccgg cacggtcgaa ttcgagggcg agccgctgct
    14881 tggcctggcc ccggaggaac gcgccgcagc cggcgtgttc ctggccttcc agtacccggt
    14941 cgagatcccc ggggtcaaca acacctactt cctgcgcgcc gcgctgaacg cccagcgcaa
    15001 ggcgcgcggc gagccggaac tggattcgat gcagttcctg aagcgcgtgc gcgagaagct
    15061 ggcggtgctg cacctgaagg acgagctgct gcatcgcggc gtcaacgaag gcttcagcgg
    15121 cggcgagaag aagcgcaacg agatcttcca gctggcgctg ctggagccga agctggcgat
    15181 cctcgacgaa accgattccg ggctcgacat cgatgcgctg aaggcggtgg cggacggcgt
    15241 caatgcgcaa cgttcgcccg ggcgcgcctt cctcgtcatc acccactacc agcgcctgct
    15301 cgattacatc cgcccggatt tcgtgcacgt gctggccgaa ggccgcatcg tccagagcgg
    15361 cgggccggaa ctggcgctgg agctggaagc gcatggctac gcctggctca gggatcgggt
    15421 cgcgcccgaa gcggcggcct gagatgggcg ccctgctcga ttcgctggcc gcgccgttcg
    15481 atggcgatgc cgcgcagcgc gcggcgctgg atgcgctgct gcaggacggc ctgccgcatg
    15541 cacgggtgga ggcgtggaaa tacacgccgc tgcgcgcgct ggagcgccgc agtttcgccg
    15601 cggccggcag cgccaccgtc accgtcgatg ccgagctgct ggccgcgatt cccgcgccgc
    15661 gcatcgtgtt cgtcaacggc cgcttcgatc ccaccctgag cgcgctggag ggcctgcctg
    15721 ccggcgcttc gctgcggccg ctctcgcagt tgctggccgc cgccgacgcg catgcagccg
    15781 gcctgctgca gcaccgctac cagcgccgcg acgaaccgtt cgcgcggctc aacgaggtgc
    15841 tggcacgcga gggcgcggtg ctgcggatcg gcgatggcgt gcaggtcgcg atgccgctgc
    15901 agctggtgtt cgtcggcgcc cccgccggcg gcgaccaggc ctggcacctg cgccagtgca
    15961 tcgcgcttgg cgagggcgcc ggcgccaccg tggtcgagca ccacctcggt ggcggcgagc
    16021 atgcccacct cggcaacgcc atcacccatg tcgaggtcgc cgctggtgcg cgccttcgcc
    16081 atgtgcgcat ccaggacgag gccagccgcg ccaccctgtt cgcgcgcacg gatgcggtgc
    16141 tggccaccgc cgccgactac cgccggatcg accttgaact cggcgcggca ctgacccggc
    16201 atgagctgaa cgtgcggctc gaaggcgaag gcgcgaacct gcaggccgac ggcgtgctgc
    16261 tggcggatgg tcgccgccac gtcgataccc gcctcggcat cgcgcacacc gcccgcgaca
    16321 ccagctgttc gctgacctgg cgcgggctcg ccggccagcg cggcagggcg gtgttccacg
    16381 gcggcatcag catcgccgcc ggtgccgacg gcagcgaggc cgcgctgtcg aacaagaacc
    16441 tgctgctgtc cgccgaggcc gagatcgatt cccagccggt gctggagatc cacgccgacg
    16501 aagtgaaggc ggcgcatggc gccaccgtcg gccagctcga tccgaccgcg ctgttctacc
    16561 tgcgctcgcg cgggctgccg gaagcggacg cgcggcgcct gctgaccgcc gggttctgcc
    16621 gcgagatcgt cgccggcgtc ggggacacgg gcttgcgcgc gcaactcggc gcggcactcg
    16681 accgggcgct ggcgcgggtg gacgcatgag catccggcag caccctcgcc ccaccccgcc
    16741 cccgccggcg gcaggcggca tgcgcgcggt cgactgggac gcggtgcgcg cggatttccc
    16801 gttgcttgcg cgccacgtcc atggcaagcc gctggtctac ctggattcgg ccaataccgg
    16861 gcagaagccg gcggcggtga tcgaggccgt cgacggcttc tatcgccgcc acaacgccaa
    16921 tgtcagccgt gcggtgcacg cgcttggcgc cgaggccacc gatgcctacg aaggcgcgcg
    16981 ccgcaagctg gccggcttcc tcaacgtgcg gcatgacgac ctggtgctgt gcagcggcac
    17041 caccttcgcg atcaacctgg tcgcctacgc gtgggcgctg ccgcgcctgc agccgggcga
    17101 cgcgatcctg ctgacgcgga tggagcacca cgcgaacatc gtgccgtggc agctggtggc
    17161 gcagcgcacc ggcgccacca tcaaggtcgc cgaactgcgc gaagacggca gcctggacct
    17221 cgacgcgctg ttcgcggcga tgaccgcgga ggtgaagctg ctcggcttca cccacgtcag
    17281 caacgtgctc ggcaccatca acccggtggc cgcgatctgc cgggaggcgc gcaggcgcgg
    17341 catcgtgacc gtggtcgacg gctcgcaggc ggcgccgcac atggcgctgg acctgcctgc
    17401 gctgggttgc gacttctacg cgatcaccgg ccacaagatg tgcggcccca ccggtaccgg
    17461 cgcgctgtgg gcgcggcgcg agcacctgca ggcgatgccg ccgttcctcg gcggcggcga
    17521 gatgatcagg gaggtgcgct tcgagggcac cgtgtacaac gatccgccgc accggttcga
    17581 ggccggcacc cccaacatcg ccggccacgt cggccttggc gcggcggtcg attacctgtc
    17641 ggcgctgggc atgcacaacg tcgccgcgcg cgaggccgaa ttgctggcgc atgccaccga
    17701 agaactgcgc aagctcgacg gcctgcgcat catcggcgaa gcaagcgaga aggcggcggt
    17761 catcagcttc ctggtggatg gcgcgcacgc gcacgacctc gccaccctgc tcgacctgga
    17821 aggcgtggcg gtgcgctccg gccaccattg cgcacacccg ctgatgcagt cgttcggggt
    17881 tccggcaacc tgccgcgcgt cattcgcgtt ctacaacacg cacgcggaag tggaggcgtt
    17941 catcgccgcg ttgcgcaagg ttcgcgggct gctggcatga gcgcgctggc ctatcgcgcc
    18001 gccaccgcgg ccgacatcga cgccgtcgtc gcgctggtgg aatcggccta ccgcggcgac
    18061 gccagccgcc agggctggac caccgaggcg gacttcctcg acggccgccg caccggcgcc
    18121 gacgacgtgg cagcctgcat cgaacgcgaa cgcagccgca tcctgctcgc ggagcgcgat
    18181 ggcgtcatgc tcgcctgcgc gcacgtggcg gaaggacaaa ccgaacaaga gcggggcgcc
    18241 ggctacttcg gcatgttctc ggtcaggccc gggctgcagg gcggcggcat cggcaagcag
    18301 gtgctggccg aggccgagcg catcgcccgc gaggactggg ggctggcggt catgcggatg
    18361 accgtgatcg acatccgcgc ggaactgatc gccttctacg aacgccgcgg ctaccaccgc
    18421 accggcatcc gcaaaccgtt cccctacggc gacgagcgct tcggcctgcc gctgcgcgac
    18481 gacctgcgct tcgaggtgct ggaaaaggaa ctggccgatg ggtgactggg tgttcgtgtg
    18541 caccaccgcg caactgctgc ccggcgagat gaccgtggcc tgggacggcg ataccgcgat
    18601 cgtggtgttc aaccacgacg gcagcttcca tgccctggaa gaccgctgca gccacgagga
    18661 ttacgagctg tccaccggca gcttcgaccc tgccaccggc tcgatcgaat gcgtcctgca
    18721 cggggcccgc ttcgatgtcc acgacggccg cgccctgtgc gcgcctgcct acgaaccggc
    18781 gaccccgttc ccggtgcggg tcgacgaggc ggggatctgg acccgcgacg atcgaaaatg
    18841 attcgcttgc gcgaatagtt tgcatttgcg aattgttctc attagcatgg ttgatgaagc
    18901 ggccagcaag tgccgcgccc cctgcccgat gagaccccga tgcctttcca gcccgcttcg
    18961 cgccgcgcgc gcctgctgtc gcgctcgccc ctcgcccttg ccctgctgac cctggttgcg
    19021 ccgctggcgc aggccgccgg gaccgacccc gccaccgatc cggccgaacc gaccgacctc
    19081 gacaaggtgc aggtggtcgg ccagcgctac ctgccggatt acgccgcgcg cgccacccgc
    19141 agcgccaccc gcaccgacac gcccctgctc gacgtccccc aggccatcac cgtggtcacc
    19201 gacaagctga tctccgacca ggccatgacc agcctgtccg atgccttccg ctacatgccg
    19261 gggatcggcc tggcgcaggg cgagggcaac cgcgacaccc cggtgctgcg cggcaacagc
    19321 tcgaccgccg atttcttcgt cgacggcatc cgcgacgacg tccagtacgt ccgcgacacc
    19381 tacaacgtgg agcgcatcga ggcgctgaag gggccgaatg cgatggtgtt cggccgcggc
    19441 ggcagtggcg gcgtgatcaa ccgcgtcacc cgccaggccg atggccaaag ccaccgcgca
    19501 ggcagcctgc aggtcggttc cggcaaccgc cgccgcggta ccgtggatgt cggcgatgcc
    19561 atcggcgaag gctcggccgg cttccgcatc accgccatgt atgaagactc ggacagtttc
    19621 cgcgacggct acacgctgga gcgccacggc atcaacccga ccttcggcgt cgatctcggc
    19681 gaacgcaccc gcctgcagct gtcctacgag cgcttccacg acgaacgcgt ggccgaccgc
    19741 ggggttccct cgctttacgg acgcccggtc gagaccgatc cctcgacgtt cttcggcaat
    19801 ccggatgcca gcccggtcga ggccaccgtg gatgcgctcg acgcggtgat cgagcacgcg
    19861 ttcgccgaca acctgagcct gcgcaaccac ctgcgctggg ccgactacga caagttctac
    19921 cagaacgtgt tcccgggcgc ggccgacgca gccacccaga ccgtgtcgat ctccgcctac
    19981 aacaactcga ccacccgcga aaacctgttc aaccagaccg acctggtgtg ggaaaccgcc
    20041 accggcggga ttcgccacac cgtgctggca ggcgccgaat tcggccgcca ggtcaccgac
    20101 aacctccgca tgaccggcta cttcggggca cccggcagca ccaccaccag catccgggtg
    20161 ccgctgtcgt cgcccacgac ttcgatcgtg ccgctgttcc ggcagagcgc gacggacgcg
    20221 gacaaccatg gcgtggcgcg ggtggccgcg gtgtacctgc aggaccaggt cgaattcacc
    20281 ccgcagtggc aggcgatcat cggcctgcgc catgacgatt tccgggtgga actgcgcaac
    20341 aaccgcaccg gcgccatcct gcgcagcgac gacggcctgc tgtcgccccg cgccggcctg
    20401 gtctacaagc cggtcgaacg ggtgtcgctg tacgccagct acagcatggc ctaccagccg
    20461 cgcgccggcg agcaactggc atcgctcagc gccagcaacg ccgcgctgga tccggagacc
    20521 ttccgcaacc gcgaactcgg tgccaagtgg gacatccgcc cggagctgca ggccagcatc
    20581 gccgtgtacc ggctggaccg cggcaacgtc gcggtcaccg atcccgccga cgccacccgc
    20641 atgatcctgg tcgacggcca gaccacggag ggcgtggaaa tcggcgtcgc cgggcgcatc
    20701 accgagaact ggcagctgat gggcggctac gcctaccagg acggcgagat caccaccacc
    20761 cagtcggcca ccgcggtcgc aggcaaccgc ctggcccagt tgccggagca ttccgcttcg
    20821 ctgtggaacc gctacgactt caaccaggcg ttcgggatcg gcctcggcat catccatcgc
    20881 gggtcgatct tcgccaacgt ggacaacacg gtcacgctgc cgtccttcac ccgcttcgac
    20941 gccgcgctgt actggaccct caacccggcc atgcaactgc agctcaacgt ggaaaacctg
    21001 ggcaacaagc gctatttcgc cagcgcccac agcaacaaca acatcagccc ggggacgccg
    21061 cgcagcgcct gggtcagcct gaacttccac ttctgaccgc gacgggcgag atgaccatgg
    21121 acgcgaacaa gcccggcagg cgcggcccgg ccgccaccta ccgctggatc cgccagttgc
    21181 acctgtgggt cggtgcctgg ggcgcgctcg ccgcgatcct gtacggcatc accggattgg
    21241 tgatgaacca ccgcttcggt gatggcgcgt ggccgcaggg cgacagcatc gaggccggca
    21301 ggaccgcgct ggcgatcccg gcgcaggcac gcaccagccc ggagcagctt tcgctgtggc
    21361 tgcgcgacgc ccggcagctg gatgcgcagg tgatccgcaa gggcggaccc ggcggccgtg
    21421 gcggcgatgc ccggcagccc ccgaaatgga acctcagcgg cggcaccgcc cgcaatgcgt
    21481 ggtcgctgga gtacgccccg ggcagcgaga cggccgagtt gaagcaaacc tcgcacacca
    21541 cccttgccgc actcaaccgc ctgcacaagg gcgtcggcgg tggcggctgg tggatcctgc
    21601 tggccgacag cttcgccatc ggcatgctcc tgctcgggct gtccgggatc tggatgtggg
    21661 cacgcgggcg cagcgcccgc gagatgctcg caagcgtgat gggactgtcg acgctcgcct
    21721 tcgtggcggt ggtcgggctg gccctgctct aggccggatc cacgggccgg acccggacca
    21781 gcaggacttc gccgccgcca tccagcacat accgcgtgcg cgcgtccgcg gcgaccagga
    21841 tggcggtgtc acccatctgc acgccgccca agccggactc gtcggcgaag cgcgcctgcc
    21901 ccgccagcac gtgcacggcc caggtttccg cgggctccac gaacaccacc atgctgccga
    21961 ccagcggccg gtgccatagc gcggcatcgg ccaggtcgcg cttccacatc aggttgaagt
    22021 cccgggtcgg gccgtcgatc agctcgccga aggccgcgcg ttcgccggcg aagcgcagct
    22081 ggtcgtgcgg tggccgcaac gcatgcgact cgccatcgtc gaagcgcagg cgcaggccgt
    22141 tgccgctgag cagcacaagc acccgttcga ccccggggaa agcggagaac ggtgcgtcgg
    22201 actcgatctc ggcgatcgac aaccgccacg cccagtcatt ggtgtcgcgt tcgtcaccgt
    22261 catggacctt gaggatttcc gaggtccagc cggcatcgtt cttccagcgc atgcggcggt
    22321 agccgcctgc acgcaggacc tgcgaagact gcggcgatgg ggaacccatc gccgcagtgt
    22381 atcgccacca cgtccgccga tcagcggcgc gcgaccgtgg ccggcatcgg cttggcggcg
    22441 accttggtgc ccgccttcgc agccgggcgg gcggcgccga gggcgatgcg gtcgcggttc
    22501 ttctcgaccg tcttcaggcc gatgcccttg accagggcca gctgctcggc gctgcggaag
    22561 gcgccgttgg ccttgcggta ggccacgatc gactcggcct tggcggggcc gacgttgacc
    22621 agcacgcggt cgatggtggc ggcgtcggcg gtgttgatgt tgacggtctc gccggcgatg
    22681 gcggcgccgg ccagcgccag cgacaggatc agcgaggaaa acagggtggt cagggacttc
    22741 atggcgggct cctcagggtg gtttggttcc gcgaccgcgg gctgcgatcc ggtgagcaca
    22801 ggttgccgat ggcgcctgcc ggcctgtatc ggcgagcacc gcacgcgcac gcccgccttc
    22861 gtcctgccgc cacgtagggg attcccgacc cgggcgctag aatgaccgtc cagccacagg
    22921 gggccgtaac gatggatgcc agcaggacca aagccttcgt cgatgccatg tgggacgagc
    22981 agatcgtccc ccagctcgtc gagtacatcc gcattcccaa caagtcgccg atgttcgacg
    23041 ccgattgggt gaagaacggc cacatggatc gcgcggtggc gttgatgcag gcgtgggcga
    23101 aggcgcagcc ggtcccgggg atgcaggtcg aggtggtgca cctgcaaggc cgcacgccgc
    23161 tgatcttcct cgacatcccg gccaccgatg gcggtggcgg cgacgactgc gtgctgctct
    23221 acggccacct cgacaagcag ccggagatga ccggctggga cgaaggcaag ggaccgtggg
    23281 tgccggtgct ggaaggcgag cgcctgtacg gccgcggcgg cgccgacgat ggctacgcga
    23341 tctacggctc gctcaccgcg atcatggcgc tgcaggcgca gggcgtggcg catgcgcgtt
    23401 gcgtggtgct gatcgaggcc tgcgaggaat ccggcagcta cgacctgccc gcctacgtcg
    23461 accacctggc cgagcgcatc ggcaagccat cactggtggt gtgcctggat tcgggctgcg
    23521 gcaactacga ccagctgtgg tgcaccacct cgctgcgcgg ccttgccggc ggcaacctga
    23581 cggtcaaggt gctggacgaa ggggtgcact ccggcgatgc ctcgggcatc gtgccgtcga
    23641 gtttccgcat catccgccaa ctgctgtcgc ggatcgagga cgaggccagc ggccgcatcc
    23701 tggtcgaagg cctgcacgtg gatatcccgg ccgaacgcaa gcagcaggcg gcgaaggtgg
    23761 cggaggtgct cggcaccgag gtgtacgaca agttcccgtt cctgccgggc atgcatccga
    23821 tggcggagga cctgaccgaa ctggtgctca accggacctg gcgaccggcg ctgtcgatca
    23881 ccggcgccga cggcatcccg ccgctggcgt cggccggcaa cgtgctgcgc ccatccaccg
    23941 cggtcaagct gagcctgcgc ctgccgccga cgctggacgg caagcgcggc ggcgaactgc
    24001 tgcagcagat cctgctgcgc gacccgccgt ccggcgccca tgtcagcctg cacctggaga
    24061 aggccagcac cggctggaac gcgccggcgc tgtcaccctg gctggcgcag gccatcgacc
    24121 aggccagcgc ggagttcttc ggcaagccgg cgatgtacat gggcgaaggc ggcaccatcc
    24181 cgttcatggg catgctcggc gagaagttcc cgggtgccca gttcatgatc accggcgtgc
    24241 ttggcccgca ctccaatgcg cacggcccca acgagttcct ggacatcccg atgggcaagg
    24301 gcgtgaccgc ctgcgtggcg cgggtgatcg ccgagcacca cgcggcgcgc ctgcgcggcg
    24361 agacctcggg cgtggcggcg atggccggga attcgtacgc ggatacgccg ggcggctgct
    24421 gctgacccgg gtggtggcga tcgccgccgc catcgtcctg ttgttcgggt acgaggcgat
    24481 gcgcacgagg aaggcctgat cccggccacg cggcatccga cgccccgccc cgtgcggggc
    24541 gtcggcgtcg tgggccctgc ataagcgcat aaccagcgct cctttcccgg tgcgcgcgca
    24601 cccgcctagc ctgcatgcag cctccttgcc tgcatcgcca tgtcgcccgc cgcgctcccc
    24661 ctgccaagcc cgctttccag cgaacacggc gccctgctgg cgcagttcgc cgcgcgcgcg
    24721 gagacagcct cgctgctgtg ggcatcgggc tacctggccg gacttgcgcg tgcgctcgat
    24781 gccccggcgc aaccgcaggt cccggcgcac ccggacgccg gcgtgcgcac gaccgtgctg
    24841 tacggcagcc agaccggcaa cgcgcggcgc gcggcggaag cgctgcatgc gcgtcttgag
    24901 gccgccggcc tgcccgcgcg cctgctgcgc gccgatgcct atccactacg cgaactgaag
    24961 gacgagcggc tgctgtacat cgcgatcagc acccagggcg acggcgaacc gccggacgat
    25021 gcgctggcct tcgtggagtt cctgtccggc aagcgcgcac cgcgtttgcc gcagctgaag
    25081 ttcgcggtgc tggcgctggg cgattcgagc tacccgcagt tctgcgccat cggccagcgc
    25141 ctcgacgcgc gcctgtccga actcggcgcg gtgcgcgttg ccgcgtgcgc caatgccgat
    25201 ctcgatgtcg agaccatcgc cgcgccatgg acggagacgg tggtcgggct tgcccaggaa
    25261 ctgctcaagc gcgcgccacc gcaggcggcc gcggcgcatg ccaccgtcgt cccgctgcgc
    25321 ccgcacgcgg tgccgcaggc gcatggccgc gagcgtccgt tcgccgcgga
//
LOCUS       DAORUK010000011        24749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_1574_length_24749_cov_17.903175, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000011 DAORUK010000000
VERSION     DAORUK010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 24749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24749
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1574_length_24749_cov_17.903175"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..818)
                     /locus_tag="PK818_01525"
     CDS             complement(147..818)
                     /locus_tag="PK818_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY81447.1"
                     /translation="MALFDFFKRKRQPAPAPKPAPAPVAAPAAAPADVPASASASDAN
                     PQEAHDFLPDPGPGVVPAHDGPERRERERRHARNGTRVLVIDDSPTIVALLKRMLQQN
                     HLQVLEAGDAETGIEVARREVPDLIFLDIVLPGMDGFSALRTLRRDPATRDVPIIMIS
                     GNAQATEQFYVQRIGADDFMKKPFSRAEVFNRIEALLDADGIPRHGSKPGTRAEAAAA
                     AAGSA"
     gene            complement(890..1780)
                     /locus_tag="PK818_01530"
     CDS             complement(890..1780)
                     /locus_tag="PK818_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81448.1"
                     /translation="MSTNTRPLPIAAALGRGLRGALQWRLLLLWALATLVCALIGALP
                     AWGWLGTLFGHSVHATAVAGGEAPALVLEALTQRDAPTSLLGASSMGAGLLMLVLSPL
                     LVGATVSAIRARTRLGFGDLLRGGIGEYGPMLRMLLWSAVPLGIALAAGSGILAANAA
                     AHKQAVVAAELDAGRYAAMAVGGVLFVLAHASLEAGRGWLAADGRLRSALKAWWRGVK
                     LLRRRPLAVLGVYLGTTLAGLLLVALLLLARPYLGDGGMPALLLGLLLSSAVASALAW
                     SRIARLSGMAALAEDAHARR"
     gene            complement(1788..3992)
                     /locus_tag="PK818_01535"
     CDS             complement(1788..3992)
                     /locus_tag="PK818_01535"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQY81449.1"
                     /translation="MHKRRAATLLLAALSLWAASTNAALPAASPAAAPPAPVACPPIA
                     LAAANASAVRVPSAADAWGGERADGAATLSNRVVAYSIDAELDPVKHTINGKQTLAWR
                     NRSAQPVCSVYLHLYLNGFEGPGSTFMTEKRASESGFRSNVATKDGEYGFIRLDKVRQ
                     DGASAKWTFVQPDGGPKTDRSVVRIDLPRPVAPGATTSFDIDFFNQLPRVVARTGYYG
                     SFHLVAQWFPKIAVLELPGERGATAPRWNAHEFHLHSEFYADFGSYDVRITVPKGYTV
                     GATGALAGPPVEKAGKVTHRYLQDDVHDFAWTADKRYAKPLVKTWNGPRGPVQVKVLY
                     PPDYASNAQPVLQATIDALQYFSDTLGPYPYKTVTAVIPPYRADEAGGMEYPTFFTAS
                     STDEDQPGTIGRFGLDFVTIHEFGHGYFYGILGSNEFEEPALDEGMNEYWDQRMMTAR
                     KQDIVLSTPWLKPYGIGTRITPFQMERLGANLDDPADPLGGNSWARLSSGSYGTVYSR
                     TATAMRQIESLVGTPAMERAMKLYYARWKFRHPSFADLREALAEGTGKPAIVNAAFDS
                     FVYGTGRVDDRVASIDSHELLPQPGYRMHEGKQVLVGSKELDKAVARLREQWEAKHAD
                     AKEWQGPFPYRSTVVVRRNGLAVPQTLRVKFADGSHRDMAVTTTGSWQRFSFVGPAKA
                     VSAQLDPDDRIHMDASELNDSRTAEPNAAPKRRWFADFTATLQSLFALLTFA"
     gene            4196..6226
                     /locus_tag="PK818_01540"
     CDS             4196..6226
                     /locus_tag="PK818_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQY81450.1"
                     /translation="MSDNQASASVETALAHALRLQASDPQLAAEQARQVLLAAPRDPV
                     ARLVLGMSHNALGEHAQALALLQPLAAEQPEAPRVQLELGIALAALARAREAVAALEH
                     ATRLQPTIPTGWLRLAEQLDACGDRARAAQAYLLHARTGMHDPRLMAAGEALSGNRLP
                     DAERLLRDRLRAFPNDVPAMRMLAELGARVGRNEQALELLERCLALAPGFAMARHNYA
                     LVLDRCNRHEDALVEVDSLLAADADNPGLQNLKAVVLGKLGSYDSAIRLYESILHKRP
                     KEPRVWMSLGHALKTEGATARAIIAYRRALELDPGFGGAWWSLANLKTVRFDAGDIAA
                     MRQQLQRSDLDDEQRLHFEFALGKALEDAGDYAGSFAHYASGNALRRTQLPYDAEQNA
                     QRRRRAQAVYTREFLAERAGAGDDDPAPVFILGMPRAGSTLVEQILSSHPQVEATMEL
                     PDIIAITRDLRARSSQPETTSYHDIVAGMDPAGFRALGERYLAGTRVQRKLGRPFFID
                     KMPNNFAHVGLIHLILPNATIIDARRHPLACCFSNFKQHFARGQAFSYDLADMGRYYV
                     DYVALMAHFDEVLPGRVHRVIYEDMVADTEVQVRALLDFCGLPFDQRCLRFFENERAV
                     RTASSEQVRRPIYREGLDQWRHFEPWLDPLKQALGPVLDHYPAVPPAGELPV"
     gene            6312..8786
                     /locus_tag="PK818_01545"
     CDS             6312..8786
                     /locus_tag="PK818_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY81451.1"
                     /translation="MRRLPLAAAVALVFSSPLAWAQSADAPASPQDKEARTLDAITVT
                     AQKRVENLQKVPISLQVLGNTQLEQQNVADFDDYAKMIPSLSYGTAGGGVFSGPGFVQ
                     VYMRGVASGGDGNHSGSQPSVGMYLDEQPITTIQGALDIHMYDIERVEALAGPQGTLY
                     GASSQAGTVRLITRKPDPSGFSAGYSVEANSISDGGLGHVLEGFVNIPVNDHAAVRLV
                     GWQKHDAGYVDNLSGTRTYPTSGIVDTNPDRAEKDYNSADTAGIRASARFDINDNWTI
                     TPQLIAQKQQAYGSAGVDPNAGNPAMGYDPGVTDLAVKHFYPETSDDRWYQAALTVEG
                     KVGNFDLTYVFSHMKRDVDSESDYSDYGFWYDTLAGYGAYFYDDNGVLINPAQYIQAT
                     DGYQKTSHELRIASPRENRVRFAAGLFWQQQQHDIHQRYRVDGLASDLSVSGYEDTIW
                     LTEQEREDRDKAVFGEVSFDVSDKLELTAGGRWFRAENALKGYFGFANGYSSQSSLDP
                     EDRYGEAGCLAQFGATWTPFNGAPCKVFDKGVSEDGWLGRLNATYQVSDSKMLYATWS
                     EGYRPGGINRRGTLPPYTSDFLTNYEFGWKTSWADNTLTFNGALFREDWKDFQFSYLG
                     QNGLTEIRNANEAQIDGLEIDLSWAATYNLQITGGFAWYDAKLTKNYCGWIKPNGEPE
                     TVCPAGTLDPNGNVVSGPQAASGTRLPVTPEFKGALNARYSFDFKGGEAYWQASLAHA
                     GERRVDMREAETALLGYLGAYTMTDLSAGWRRNNWSIDVFVKNVFDERAEMTRFAQCA
                     ALTCGNQPYTVVAQPRTIGIRFSQEF"
     gene            complement(8856..9779)
                     /locus_tag="PK818_01550"
     CDS             complement(8856..9779)
                     /locus_tag="PK818_01550"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY81452.1"
                     /translation="MRRGLLAVALALAVAAPALAQDAQQPPKRKSAKEIIDAAPASAW
                     RDLDPANTLYMELPAGRVIIELAPGFAPEHVANIKTMAKGGFWDGLTIYRSQDNFVVQ
                     FGDAEGEEAGKAKPYPTGTRTRLPAEFTRSTEGARFDKLPDIDGWAPQVGFIDGMPAA
                     RDPKAGKAWITHCYGVLGAGRNNAPDSSIGAELYVVIGQAPRALDHQLSVVGRVVKGM
                     DLLSTIPRGPEPMGFYQKPEQRAPIKAIRLASEVPAAERTPLQLLRTDSQAFIDATEA
                     RRNRVDDFYTVPAGHIDLCNVALPVRTPPAK"
     gene            9955..10314
                     /locus_tag="PK818_01555"
     CDS             9955..10314
                     /locus_tag="PK818_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HQY81453.1"
                     /translation="MDETLLRKFHKELSAGTVSLALLAVVAAGSEAMYGYQIAKRLEQ
                     LGDGVLAGKQSALYPVLRNLEGAGLLESFVEPSMSGPPRRYYRVTQAGREALTDWSAA
                     WRATRDSLDAVLKEASA"
     gene            10311..11216
                     /locus_tag="PK818_01560"
     CDS             10311..11216
                     /locus_tag="PK818_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HQY81454.1"
                     /translation="MNADLPTTIPAYLDRLRRALEGCDPALAQDALYDAEDYLRSELA
                     AHPGKTEAEVIAAVAGSYGAPDEVADIYRDTEVKVQTALRAPLPAPRESLLGRFFGVL
                     VDPHTYGSLFYMLLAMATGTFYFSWVVTGASLSLGLAILIIGIPFVILFIGATRILAL
                     VEGRIVEVMLGERMPRRPLYSDKGLPVLQRIGAMFTDPRTWGTMLYFLAMLPLGITYF
                     TVVVVALSLGAAMIALPFGHWFGFGEYIRLDGFSLFDPSSMWTWPLSILAGVLLLFAT
                     LHLARFIGRMHGKLAKAMLVSSRAY"
     gene            complement(11336..12709)
                     /gene="phaZ"
                     /locus_tag="PK818_01565"
     CDS             complement(11336..12709)
                     /gene="phaZ"
                     /locus_tag="PK818_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HQY81455.1"
                     /translation="MLLYQWHELWRAGLAPYTYFADAGAKMYSAADSWLTAVPGAQRT
                     AAAFELMYRLGKDYEKPEFGIHQIEVEGLAIPVVERVALEKPFCRLLRFKRYHDQADG
                     LARMKDDPTVLVVAPLSGHHATLLRDTVRTLLADHKVYITDWTDARMVPTAEGQFGLD
                     DYVAYIEEFIRHIGTDNLHVISVCQPTVPVLAAISLMAGRGESLPRSMVMMGGPIDPR
                     ESPTSVNNLATRQPLSWFENNVIHPVPANYPGSGRQVYPGFLQHLGFVAMNPERHATS
                     HWDFYQDLLRGDLEDAQSHRKFYDEYNAVLDMPARYYLDTIQKVFQEFLLPRGLWDVA
                     GERVDPSKIRDCALFTIEGELDDISGAGQTRAAHKLCTGIAAEDQKHLTAEGAGHYGI
                     FSGRRWRTQVYPQVKAFIAAHAGSNPPVVAATSGAMPARKPAARASVATKATVAKPQR
                     IAKKSTR"
     gene            complement(12826..13419)
                     /locus_tag="PK818_01570"
     CDS             complement(12826..13419)
                     /locus_tag="PK818_01570"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY81456.1"
                     /translation="MTKTYDRAYFDRWYRGSEVGGSARLARKVALAVATAEYHLERPL
                     RSVLDIGCGEGGWRAPLLKLRPRASYLGFDASEYATGRYGRARNLHLARFGDFEFLRP
                     CAPVDLLVCSDVLHYLPTRELDRGLPALADLCGGVAFIEAFAREDEAVGDDHGFIARP
                     AGFYRKRLRAVGLHPLGSHCWLSPARVAQAAALELPL"
     gene            13461..13880
                     /locus_tag="PK818_01575"
     CDS             13461..13880
                     /locus_tag="PK818_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011258679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HQY81457.1"
                     /translation="MAYLLTKTAHLVFVIAWMAGVFYLPRILVNIAEAGGAADVRARL
                     LLMGRRLYRFGHVMFALATLLGLVLWLRFGVTGGWLHAKLLLVTLVLLHYLAAGRWLR
                     GVANGRPLPSSTALRWFNEIPVLLLAAIVWLVLAKPF"
     gene            complement(14030..15727)
                     /locus_tag="PK818_01580"
     CDS             complement(14030..15727)
                     /locus_tag="PK818_01580"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HQY81458.1"
                     /translation="MRPTFLALAFATTLVAGGCAASGGHPRAGAHATGLQVEVPRIAR
                     PGGETAAWWFRDGAAQAAARGAMDGRAKNLIVFLGDGMSLATVTAARVFEGQRNGGSG
                     EENRLSWETFPATALSKTYNTNSQTPDSAGTMSAIATGVKTRAGVLSIGQTAPRGDCA
                     GALAAPMLSLWELASASGMATGVVTSTRVTHATPGATFAHSPERNWENDTELSDAARA
                     AGCVDIARQLVETPFAGGFDVLMGGGRQNFMPVSQRDPEYDDKVGLRLDERDLVAEWQ
                     QRHPGGAYVWNLRQLQDAPVDAPLLALFEPDHMQFEHDRTRDAAGEPSLAEMTRAAIT
                     RLSRHPGGYVLLVEGGRIDHAHHYGNAYRALADTVAMSDAVRAVLEATSAADTLVLVT
                     ADHAHTMTFAGYPVRGNPILGKVRGTGAEDAEVGLAADATGLPYTTLGYANGPGYTGA
                     SSAQPAGPKRFFHAGRDYLPAEGRPDLREVDTEHPDFMQEATVPLASESHGGDDVGIW
                     ASGPGSEAVRGSIEQNAIFHILLQATPRLRTALCAKDLCDANGVPVQLPQIAEFRPR"
     gene            15870..17189
                     /locus_tag="PK818_01585"
     CDS             15870..17189
                     /locus_tag="PK818_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HQY81459.1"
                     /translation="MFDWWFRIKFWKRVVAGFVLGALAGWAFGPQAETWFGPLGDLYV
                     TLIKMIAVPLVFFAVINAVASLGGQKSIAALAGRTFAWFALTAVLAVGVGLAFGWMLQ
                     PGAGVGVLQVASDYTPKSVPGVLDMLLNLVPSNPFQALSGAASAETADGMDVLVPRNG
                     TVLQVIFFAGLVGFAIVRLGGRVAGIRALAGEASEVMIQVTRFVLEFTPLGTFGLIAA
                     LVGGYGFEQLKPLFNFVVALYAACAFHIIVVYGGLLLAHGLNPLKFFRGAAPGMQVAF
                     VASSSFAALPASLRSATHNLGVDKDYAAFAVPLGATIKMDGCGAIYPALAAVFISQYA
                     GIELSVSQYVVIALASVLGSFGTAGVPGTAVIMATVVLSAAGLPLEVIGYLYAIDRVL
                     DMMRTMTNVTGQMLVPVLVAKQSGMLDRAVYDAAPTDVGLDERGQAG"
     gene            complement(17225..17644)
                     /locus_tag="PK818_01590"
     CDS             complement(17225..17644)
                     /locus_tag="PK818_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81460.1"
                     /translation="MGRTILGAVAGLLVAFFTIMVVEFAGHQVYPPPPGLDPMVPADM
                     AMIMQSMPLGAMLLVVVAWLTGAFDGGFVAALVARGNRPRVAAVVPALLVMAGVVGMV
                     VQLPGHPLWMSIAGLLLPIPVALAGAGLASRRKPAAR"
     gene            complement(17776..18225)
                     /locus_tag="PK818_01595"
                     /pseudo
     CDS             complement(17776..18225)
                     /locus_tag="PK818_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            complement(18602..19072)
                     /locus_tag="PK818_01600"
     CDS             complement(18602..19072)
                     /locus_tag="PK818_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HQY81461.1"
                     /translation="MALLAACGGGQALRPAAPAAQAWPAVAPADPARANAVLMRAISL
                     VGTPYRYGGNTPEGGFDCSGLVNYVYRDMLDLRLPRTSRELFAMPHAPIATDRLAGGD
                     LVFFGSDGNVSHVGIYVGEGRFVHAPSSGGTVRLDRLDGHWWRDHYRGARRLLN"
     gene            19202..19684
                     /locus_tag="PK818_01605"
     CDS             19202..19684
                     /locus_tag="PK818_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY81462.1"
                     /translation="MKIEKDRVVRFHYAVAEAGQEAVENSKDGGQPLAILFGHGNIIP
                     GLEKAMDGREAGDSFKASVAAADAYGERREGLGQRIPKKHFGAQKLAPGMQVVLQTNF
                     GPRAVTIEKVGMSVVDVDLNHPMAGKDLEFDIEIVEVREASAEELAHGHVHGDGGHHH
                     "
     gene            19844..21529
                     /locus_tag="PK818_01610"
     CDS             19844..21529
                     /locus_tag="PK818_01610"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HQY81463.1"
                     /translation="MAAAEALRPLQLHERIQTLDVIRGFALLGIFLMNVEWFNRPIAD
                     LGAGVPAGLAGPDLVVAWLIHTFVASKFWPMFSLLFGMGFAVMLQRARASGSAFVATY
                     ARRTAALAAFGLLHGVLLWPGDILFAYAVTAAALLLTLFGTPKHWLALLLPFAVLAAI
                     PATSSAGVAIVVLLFAALVALYLRDERSFRGWPRIALVVLAMALLMLVGGAVTLAKVG
                     ADKGAPLVVGALLLVVVAWLAGRHRGPPAQRALRAGVAIFLLPTLMMLAGGFYMMFGP
                     PAQEGARAGAERPVAATPATAGKQPRAAAPSPVEDAGAKARAAQRAEVAKRLAERKAQ
                     AAEETRIMSGGTYAEAVRFRAGAYLRDLGQSVGLSVIALGLFLLGGWFIQSGMMVQPQ
                     RHLPAFRKLAVTGMLAGGALSIAASLVSLRPLERAADAPPDALWQLANALHMMGGLPL
                     MLGYVACLVLALQAATGQRLLGWLAPAGRMALTNYLMQSLIASLFFYGYGLGHWGMPR
                     AQQAVFVVVVFGLQVLLSHWWLARFRYGPIEWLWRWMTYGTRPALRLAASVAA"
     gene            complement(21602..>22294)
                     /locus_tag="PK818_01615"
     CDS             complement(21602..>22294)
                     /locus_tag="PK818_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007967835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HQY81464.1"
                     /translation="DGKGVKSYVHVGTNWINAAWYANAMYYGDGGSGYLPLVSLDVAG
                     HEMSHGVNQAEANLAYSNDSGGLNEANSDIMGTMVEFYANNANDPGDYKIGEELSSSP
                     LRYMYDPNLDGNASYDCYPAGGLGGVDPHYSSGPANHFFYLLAEGTTSLTRTCAPSDS
                     KNATGNGSLAGIGRTAAAAIWYRAIRDYMTSSTTYPGARVATLQAATALYGAGSAQYN
                     AVAAAWSAINVN"
     assembly_gap    22295..22394
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22395..22966)
                     /locus_tag="PK818_01620"
     CDS             complement(<22395..22966)
                     /locus_tag="PK818_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007967835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HQY81465.1"
                     /translation="MRTSTRLLTAAVLAALSSGAFAAGPAERALGHLRANGAIAKAAD
                     GDAFIARSVNVDADGTEHVRFDRTYRGMPVIGGDFVVHSRNGQVKGTSMTLSSSGRPG
                     LSGRISADQATITAGAEFGTDFTGVQEARKVVFARNTSPRLAYEVVFTGTKRDQTPTE
                     MHYFVDASTGKILDRWDMVHTAKPGGGGTGG"
     gene            23222..24499
                     /locus_tag="PK818_01625"
     CDS             23222..24499
                     /locus_tag="PK818_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY81466.1"
                     /translation="MPAAPQQGIGDSDCLVVGAGVSGLATALALLADGRGVLVVDAGE
                     IGGGASHGNCGTLTPSHAPPLAAPGTIPRALRWMLTPDAPLYVPPRFDPALWRWLLGF
                     AMRCNHRDWRASTRAKSALLNDSRQRIADWVRDYDLQCEFVESGEDYVFHDARAMEHE
                     LRDLPLLREFGIEAEVIDGPAYERQEPALKPGVAGAIRFAGDAALRPDRYVAELARAV
                     RERGGTIIEHCALQSLEAGTDGVRAVTARGAVRARDAVIATGAWSPTLARAIGLPWLR
                     KAIQPGKGYSMTYSAPAVVPKRPLTLREVSVCVTAWGSGFRLGSTMEFSGYDSTLNER
                     RLGALERGARKYLHHPVGPALRERWFGWRPMSRDDIPLIGRAPGHSHLWLATGHGMMG
                     VGMSAGTGQLLADMVAQRRPAVDPSAFDPARFA"
     gene            24499..>24749
                     /locus_tag="PK818_01630"
     CDS             24499..>24749
                     /locus_tag="PK818_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HQY81467.1"
                     /translation="MEPVYTLHRGSAPLLVSLPHDGSAIPDALAARMTPEARRAPDTD
                     WHVSRLYAFARELGASILVPRHSRYVIDLNRGEDDTSLYP"
BASE COUNT         3633 a         8239 c         8829 g         3948 t
ORIGIN      
        1 gcctgaatgc cgatgccttc gtcaacttcg cgctggatgc ggacggcaag atccgcgaga
       61 tgcggatgca gccggtgtcg ccgctgaccg acttcagctt cgacttccag gacctgcggc
      121 tggcaccggt gccggtgaag taacgctcag gcgctgccgg ccgcggccgc ggccgcttcc
      181 gcgcgcgtgc ccggcttgct gccgtggcgc gggatgccgt cggcgtcgag cagcgcctcg
      241 atccggttga acacctcggc gcgcgagaac ggcttcttca tgaagtcgtc ggcgccgatg
      301 cgctgcacgt agaactgctc ggtggcctgc gcgttgccgc tgatcatgat gatcggcacg
      361 tcgcgggtgg ccgggtcgcg gcgcagggtg cgcagcgcgc tgaagccgtc catgccgggc
      421 agcacgatgt cgaggaagat caggtccggc acctcgcggc gcgcgacctc gatgccggtt
      481 tcggcgtcgc cggcttccag cacctgcagg tggttctgct gcagcatgcg cttgagcagg
      541 gcgacgatcg tcggcgaatc gtcgatcacc agcacgcggg tgccgttccg cgcatgccgg
      601 cgttcgcgtt cgcggcgctc ggggccatcg tgcgcaggga caacccccgg ccccggatcg
      661 gggaggaaat cgtgggcctc ctgcggattg gcgtcactgg cgctggccga ggccgggacg
      721 tcggctggcg cagcagcggg ggctgccacg ggtgccggtg ccggcttggg tgccggcgcg
      781 ggttgccgct tgcgcttgaa gaaatcgaac agggccacgt cgtctccggg gggcgttcgc
      841 cacgggcaat cccggatggt aatccgggcg cgcgggcttg tcgccctcgt caacggcggg
      901 cgtgggcgtc ctcggccagt gccgccatcc cggacaggcg ggcgatccgg ctccacgcca
      961 gcgctgaggc gacggcgctg gacagcagca ggcccagcag cagcgccggc atcccgccgt
     1021 cacccaggta cgggcgcgcc agcagcagca gtgcgaccag cagcaggccc gccagggtgg
     1081 tgcccaggta cacgccgagc accgccagcg ggcgacggcg cagcagtttc accccacgcc
     1141 accatgcctt cagcgccgag cgcaggcggc catccgcggc cagccagccg cggccggcct
     1201 caaggctggc atgcgccagc acgaacagca ccccgccgac cgccatcgcg gcgtagcggc
     1261 cggcatccag ttcggcggca accaccgcct gcttgtgcgc ggcggcgttg gccgcgagga
     1321 tcccgctgcc cgccgccagc gcgatcccca gcgggaccgc cgaccacagc agcatccgca
     1381 gcatcggccc gtattcgccg atcccgccgc gcagcaggtc gccgaagccc aggcgcgtgc
     1441 gtgcgcggat cgcggacacc gtggcgccga ccagcagcgg cgacagcacc agcatcagca
     1501 ggcccgcgcc catgctgctg gcgccgagca gggacgttgg cgcgtcgcgc tgggtcagcg
     1561 cttccagcac cagggccggg gcctcgccac cggcgactgc ggtggcgtgc acggagtgcc
     1621 cgaacagggt gccgagccag ccccaggccg gcagcgcgcc gatcagggcg cacaccagtg
     1681 tggccagcgc ccacagcagc agcagccgcc attgcagggc accgcgcagg ccacggccaa
     1741 gggcggcggc gatcggaagc gggcgggtgt tggtggacat gggcgcctca ggcgaaggtg
     1801 agcagcgcga acagcgactg cagggtggcg gtgaagtcgg cgaaccagcg ccgcttcggc
     1861 gcggcattgg gctcggcggt gcggctgtcg ttgagttcgg aggcatccat gtggatgcgg
     1921 tcgtccggat ccagctgcgc cgagaccgcc ttcgccgggc cgacgaacga gaagcgttgc
     1981 cagctgccgg tggtggtcac cgccatgtcg cgatggctgc catcggcgaa cttcacgcgc
     2041 agggtctgcg gcaccgccag gccattgcgg cggaccacca cggtgctgcg gtacgggaag
     2101 ggcccttgcc attccttcgc gtcggcatgc ttggcttccc actgctcgcg caaccgggcg
     2161 accgccttgt ccagttcctt gctgcccacc agcacctgct tgccttcgtg catccggtag
     2221 cccggttgcg gcagcagctc gtggctgtcg atgctggcca cgcggtcgtc gacccggccg
     2281 gtgccgtaga cgaatgaatc gaacgcggca ttgacgatcg cgggcttgcc ggtgccttcg
     2341 gccagcgcct cgcgcaggtc ggcgaacgag gggtggcgga acttccagcg cgcgtagtac
     2401 agcttcatcg cgcgttccat cgccggggtg ccgaccagcg attcgatctg gcgcatggcg
     2461 gtggcggtgc gcgaatacac ggtgccatag ctgccgctgg acaggcgcgc ccaggagttg
     2521 ccgcccagcg ggtcggccgg gtcgtcgagg ttggcgccca ggcgctccat ctggaacggg
     2581 gtgatccggg tgccgatccc gtagggcttc agccagggcg tgctcaacac gatgtcctgc
     2641 ttgcgcgcag tcatcatgcg ctggtcccag tactcgttca tgccctcgtc gagcgcgggt
     2701 tcctcgaatt cgttggagcc gaggatgccg tagaagtaac cgtggccgaa ttcgtggatg
     2761 gtcacgaagt cgaggccgaa acggccgatg gtgccgggct ggtcctcgtc ggtgctgctg
     2821 gcggtgaaga aggtcgggta ctccatgccg ccggcttcgt ccgcgcggta cggcgggatc
     2881 accgcggtca cggtcttgta cgggtacggg ccgagggtgt cggagaagta ctgcaacgcg
     2941 tcgatggtgg cctgcagcac cggttgcgca ttcgatgcgt agtccggcgg atacagcacc
     3001 ttgacctgca ccgggccgcg cggcccgttc caggtcttca ccagcggctt ggcgtagcgc
     3061 ttgtccgcgg tccacgcgaa gtcgtgcacg tcgtcctgca ggtagcggtg ggtgaccttg
     3121 ccggcctttt ccaccggggg gccggcgagg gcgccggtgg cgcccacggt gtagcccttg
     3181 ggcacggtga tgcgcacgtc gtagctgccg aagtccgcgt agaactcgct gtgcaggtgg
     3241 aattcgtggg cgttccagcg gggcgcggtg gcaccgcgtt cgcccggcag ctccagcacc
     3301 gcgatcttcg ggaaccactg cgcgaccagg tggaagctgc cgtagtagcc ggtacgcgcg
     3361 accacccgcg gcaactggtt gaagaagtcg atgtcgaagc tggtggtggc accgggcgcg
     3421 accgggcgcg gcaggtcgat gcggaccacg ctgcggtcgg tcttcgggcc gccgtcgggc
     3481 tgcacgaacg tccacttcgc gctggcgccg tcctgccgca ccttgtccag gcggatgaag
     3541 ccgtactcgc cgtccttggt ggcgacgttg ctgcggaagc cgctttcgct ggcccgcttc
     3601 tcggtcatga aggtgctgcc gggtccctcg aagccattga ggtagaggtg caggtacacg
     3661 ctgcacaccg gctgcgcgct gcggttgcgc cacgccaggg tctgcttgcc gttgatggtg
     3721 tgcttgaccg ggtcaagttc ggcgtcgatg gaatacgcca ccacccggtt ggacagggtg
     3781 gcggcgccgt cggcgcgctc gccgccccac gcgtcggccg cgcttggcac ccgcaccgcg
     3841 gaggcattcg cggcggccag tgcgatcggc gggcaggcaa ccggcgcggg tggtgccgcg
     3901 gcgggcgatg ccgccggcag tgcagcgttc gtcgatgccg cccagagcga cagggcggca
     3961 agcaggaggg tggcggcacg gcgcttgtgc atgggcaggt tccggcgggg aagccaaaca
     4021 gagcggcaag cgtgtgcgat ggcggcatcg gtggcaagat gccgtcggtc atgcatggcc
     4081 tggtgcgaaa tgcacggcgg cgcgcggcca ttgcgaccaa gtcgcatccg cacgcgcgct
     4141 tggattcggt ggccgtgtcc gcaggcgcgg cgtgcatccg ctgcgaaggg acacgatgag
     4201 cgacaaccaa gccagcgcca gcgtcgaaac cgccctggcg catgcgctgc gcctgcaggc
     4261 cagcgacccc cagctggcgg ccgaacaggc gcgccaggtc ctgctcgcag cgccacgcga
     4321 cccggtcgcg cggctggtcc ttggcatgtc gcacaacgcg ctcggcgaac atgcgcaagc
     4381 gctggcgctg ctgcagccgc tggccgcgga acagccggag gcaccacggg tccaactgga
     4441 actcgggatc gcgctcgccg cgctggcgcg tgccagggag gcggtcgccg cgctggaaca
     4501 cgcgacccgg ctgcaaccca cgatcccgac cggatggttg cgcctggccg agcagctgga
     4561 tgcctgcggc gaccgcgcgc gcgccgccca ggcctacctg ctgcatgcgc gcaccggcat
     4621 gcacgaccca cggctgatgg ccgcgggcga ggcgctgtca ggcaaccgcc tgcccgatgc
     4681 cgagcgcctg ctgcgcgatc gcctgcgcgc ctttcccaac gacgtcccgg cgatgcggat
     4741 gctggccgaa ctcggggccc gggtcgggcg caacgagcag gcgctggagc tgctcgagcg
     4801 ctgcctcgcg ctggcgcccg gctttgcgat ggcgcgccac aactatgcgc tggtgctgga
     4861 tcgctgcaac cgccacgagg atgcgctggt cgaggtggac agcctgctgg cggcggacgc
     4921 ggacaatccc ggcctgcaga acctgaaggc ggtggtactg ggcaagctcg gcagttacga
     4981 cagcgcgatc cgcttgtatg agtcgatcct gcacaagcgg cccaaggagc cgcgggtgtg
     5041 gatgagcctg ggccacgcac tcaagaccga gggcgcgacc gcgcgcgcga tcatcgccta
     5101 ccgccgtgcg ctcgaactcg accccgggtt cggcggcgcg tggtggagcc tggccaacct
     5161 caagaccgtg cgtttcgatg ccggcgacat cgctgcgatg cgccagcagc tgcaacgaag
     5221 cgacctcgac gacgagcagc gcctgcattt cgagttcgcg ctcggcaagg cgctggagga
     5281 tgccggcgac tacgccgggt cgttcgcgca ttacgccagc ggcaacgccc tgcggcgcac
     5341 ccagctgccg tacgacgcgg agcagaacgc ccagcgccgg cgccgcgcgc aggcggtgta
     5401 cacgcgcgag ttcctggccg aacgcgccgg tgccggcgac gacgacccgg cgccggtgtt
     5461 catcctcggc atgccgcgcg ccggctccac cctggtggag cagatcctgt ccagccatcc
     5521 gcaggtggaa gcgacgatgg agctgccgga catcatcgcc atcacccgcg acctgcgggc
     5581 ccgatcaagc cagccggaga ccacgtccta ccacgacatc gtggcaggca tggacccggc
     5641 cgggttcagg gcgctgggcg agcgctacct cgccggcacc cgggtgcagc gcaagctggg
     5701 gcgcccgttc ttcatcgaca agatgccgaa caatttcgcg cacgtcgggc tgatccacct
     5761 gatcctgccg aacgcgacga tcatcgacgc gcgccggcac ccgctggcgt gctgtttctc
     5821 aaacttcaag cagcacttcg cccgcggcca ggccttcagc tacgacctgg ccgacatggg
     5881 ccgctactac gtcgactacg tggcgctgat ggcgcacttc gacgaggtgt tgccgggccg
     5941 ggtccatcgc gtcatctacg aggacatggt ggccgacacc gaggtccagg tgcgcgcctt
     6001 gctggacttc tgcggcctgc cgttcgacca gcgctgcctg cgcttcttcg agaacgagcg
     6061 cgcggtgcgc accgccagtt cggagcaggt gcggcggccg atctaccgcg aaggactgga
     6121 ccagtggcgg catttcgagc catggctgga tccactgaag caggcgcttg gcccggtgct
     6181 cgaccactac ccggcggtac cgccggcggg ggagttgccg gtttgaggtg aaaattgcgt
     6241 aaagttggcg tgaatccttg ggaggggacc tcgcaatgca gactcgcacc cgtacgaccc
     6301 gccgttccgc catgcgccgc ctgccgctgg cagccgcggt tgcgctggtg ttttcctcgc
     6361 cgctggcatg ggcgcagtcc gccgatgcgc cggcgagccc gcaggacaag gaggcgcgca
     6421 ccctggatgc aatcacggtg accgcgcaga agcgcgtcga gaacctgcag aaggtgccta
     6481 tcagcctgca ggtgctgggc aatacccagc tcgaacagca gaacgtcgcc gatttcgacg
     6541 actacgcgaa gatgatcccc agcctgtcgt acggcaccgc cggtggcggc gtgttctccg
     6601 gcccgggctt cgtgcaggtg tacatgcgcg gcgtggccag cggtggcgat ggcaaccact
     6661 ccggctcgca gcccagcgtg ggcatgtacc tggacgagca gccgatcacc accatccagg
     6721 gcgcgctcga catccacatg tacgacatcg agcgcgtcga ggcgctggcc ggcccgcagg
     6781 gcaccctgta cggcgccagc tcgcaggccg gcacggtccg cctgatcacc cgcaagccgg
     6841 atccgtccgg attctcggcc ggctacagcg tcgaggccaa cagcatcagt gacggcggcc
     6901 tcggccacgt gctggagggc ttcgtcaaca tcccggtcaa cgaccacgcc gcggtgcgcc
     6961 tggtcggctg gcagaagcac gatgccggct acgtcgacaa cctcagcggc acccgcacct
     7021 atcccacctc gggcatcgtc gacaccaacc cggaccgcgc cgagaaggac tacaacagcg
     7081 ccgacaccgc cggcatccgt gcctccgcgc gcttcgacat caacgacaac tggaccatca
     7141 ccccgcagct gatcgcgcag aagcagcagg cctacggaag cgccggcgtc gacccgaacg
     7201 ccggcaaccc ggcgatgggc tacgaccccg gggtaaccga cctggctgtc aagcatttct
     7261 atccggaaac ctcggacgac cgctggtacc aggcggcgct gacggtggag ggcaaggtcg
     7321 gcaacttcga cctgacctac gtgttctcgc acatgaagcg cgacgtcgat tccgaatccg
     7381 actacagcga ctacggcttc tggtacgaca ccctggccgg ctacggcgcg tatttctacg
     7441 acgacaacgg agtcctgatc aatccggcgc agtacatcca ggcgactgat ggctaccaga
     7501 agaccagcca tgagctgcgc atcgcatcgc cgcgcgagaa ccgagtccgt tttgcggcag
     7561 gtctgttctg gcagcagcag cagcacgaca tccaccaacg ttaccgggtc gatggtctgg
     7621 cttcggacct gtcggtcagt ggctacgagg atacgatttg gttgactgaa caggaacgcg
     7681 aggatcgcga caaggcagtg ttcggtgagg tcagtttcga cgtctccgac aagctggagc
     7741 tgactgccgg cgggcgctgg ttccgcgccg aaaatgcgct gaagggatat ttcggttttg
     7801 ccaatggata ctcctcgcaa tccagcctgg acccggagga tcgttatggc gaagcgggtt
     7861 gcctggcgca attcggagcg acttggacgc cgttcaatgg ggcgccgtgc aaggtgttcg
     7921 acaagggcgt tagcgaggac ggttggcttg gtcggctcaa tgcgacctac caggttagcg
     7981 atagcaagat gctgtatgcg acgtggtcgg agggttaccg acccggcggc atcaaccgcc
     8041 gcggcacgtt gccgccttat acctccgact tcctgacaaa ttacgagttt ggctggaaga
     8101 ccagttgggc tgacaacacc ctgaccttca acggcgcgct gttccgcgag gactggaagg
     8161 acttccagtt ctcctacctt ggccagaacg ggttgaccga gatccgcaac gccaacgagg
     8221 cgcagatcga cggcctggag atcgacctca gctgggcggc cacctacaac ctgcagatca
     8281 ccggcggctt cgcctggtac gacgccaagc tgaccaagaa ctactgtggc tggatcaagc
     8341 cgaatggtga gccggaaacc gtgtgcccgg cgggcacgct ggatccgaat ggcaacgtgg
     8401 tcagcggccc gcaggcggca tccggcaccc gactgccggt cacgccggag ttcaagggcg
     8461 cgttgaacgc gcgttacagc ttcgacttca agggcggcga ggcctactgg caggcgtcgc
     8521 tggcgcatgc cggcgaacgc cgggtcgaca tgcgcgaggc ggaaaccgcg ttgctggggt
     8581 acctcggtgc ctacacgatg accgacctgt ccgccggatg gcgccgcaac aactggtcga
     8641 tcgacgtgtt cgtcaagaac gtgttcgacg aacgcgccga gatgacccgc ttcgcccagt
     8701 gcgcggcgct gacctgcggc aaccagccct acaccgtcgt cgcccagccg cgcaccatcg
     8761 gcatccgctt cagccaggag ttctgaagcg ggctggcaag ctgcgatgca acgaaaaagg
     8821 cggccactgg ccgccttttt cgtggggagg accggttact tcgccggcgg cgtgcgcacg
     8881 ggcagggcca cgttgcacag gtcgatgtgg ccggcgggga cggtgtagaa atcgtccacc
     8941 cggttgcggc gggcttcggt ggcgtcgatg aaggcctggc tgtcggtgcg cagcagctgc
     9001 agtggcgtgc gttccgcggc gggcacttcg ctggccaacc ggatcgcctt gattggcgcg
     9061 cgctgttcgg gcttctggta gaagcccatc ggctccgggc cgcgcgggat cgtgctcagc
     9121 aggtccatgc ccttgaccac ccggccgacc acgctgagct ggtggtccag cgcgcgcggg
     9181 gcctggccga tgacgacgta gagttcggca ccgatgctgc tgtccggcgc gttgttgcgg
     9241 ccggcgccga gcacgccgta gcaatgggtg atccaggcct tgccggcctt ggggtcgcgc
     9301 gcggcgggca tgccgtcgat gaagccgacc tgcggcgccc agccgtcgat gtcgggcagc
     9361 ttgtcgaagc gtgcgccctc ggtgctgcgg gtgaattcgg ccggcaggcg ggtgcgggtg
     9421 ccggtggggt agggcttggc cttgccggct tcctcgccct cggcatcgcc gaactggacc
     9481 acgaaattgt cctgcgagcg gtagatggtg agcccgtccc agaagccgcc tttggccatg
     9541 gtcttgatgt tggccacgtg ctcgggcgcg aagcccgggg cgagctcgat gatgacgcgg
     9601 cctgcgggca gctccatgta cagggtgttg gccgggtcga ggtcgcgcca ggcactggcc
     9661 ggggcggcat cgatgatctc tttcgccgac ttgcgcttcg gtggctgctg cgcgtcctgg
     9721 gcaagggcag gggcggcaac ggcgagggca agggcaaccg caagcaatcc acgacgcatc
     9781 tggtcgactc ctgagcgaaa accgaagcat agcgcccccg tgcagcaggc ccggatgagt
     9841 ttgggggcga ccggatccag cgtgagaggt accggcatcg ggcgcatgac tactggcata
     9901 ttcactatat cgaaatcaac agtagtatcg actccagatt agtggagcct gccaatggac
     9961 gagacgctgc tacgcaaatt ccacaaggag ctgagcgccg gaaccgtgtc cctggccttg
    10021 ctggcggtgg tcgccgccgg cagcgaggca atgtacggct accagatcgc caagcgcttg
    10081 gagcagctcg gcgacggcgt gctggccggc aagcagagcg cgctgtaccc ggtgctgcgc
    10141 aacctggagg gcgccggctt gctggaaagc ttcgtggaac cctcgatgag cggcccgccg
    10201 cgccgctact accgcgtcac ccaggccggg cgcgaagcgc tcaccgactg gagcgccgcc
    10261 tggcgtgcca cccgcgattc cctcgatgcc gtgctcaagg aagccagcgc atgaacgccg
    10321 acctgccgac caccatcccc gcctacctgg accgcctgcg gcgcgcgctg gagggctgcg
    10381 acccggcgct ggcgcaggac gcgctgtacg acgccgagga ctacctgcgc tcggaactgg
    10441 ccgcgcaccc gggcaagacc gaagccgagg tgatcgccgc ggtcgccggc agctacggtg
    10501 cgccggacga agtggcggac atctaccgcg acaccgaggt gaaggtgcag accgcgctgc
    10561 gcgcgccgct gccggcgccg cgcgagtcgc tgctcggccg cttcttcggc gtgctggtgg
    10621 atccgcatac ctacggttcg ctgttctaca tgctgctggc gatggccacc ggcaccttct
    10681 acttcagctg ggtggtcacc ggcgcatcgc tgtcgctggg cctggcgatc ctgatcatcg
    10741 gcatcccgtt cgtgatcctg ttcatcggcg cgacgcgcat cctggcgctg gtggaagggc
    10801 gcatcgtcga ggtgatgctg ggcgaacgga tgccgcgcag gccgctgtac agcgacaagg
    10861 gcctgccggt gctgcagcgg atcggcgcga tgttcaccga cccgcgcacc tggggcacga
    10921 tgctgtactt cctggcgatg ctgccgctgg gcatcaccta cttcaccgtg gtggtggtgg
    10981 cgctcagcct cggtgcggcg atgatcgcgc tgccgttcgg ccactggttc ggcttcggcg
    11041 aatacatccg gttggatggc ttcagcctgt tcgacccgag ctcgatgtgg acatggccgc
    11101 tgtcgatcct ggccggcgtg ctgctgctgt tcgccaccct gcacctggcc cgcttcatcg
    11161 gccgcatgca cggcaagctg gcgaaggcga tgctggtcag cagccgcgcc tactgaggcg
    11221 gcgccggcag ccacgaaagg ggacgcccgc ttctgcgggg tgcgtgggaa attcgagcgg
    11281 cgaatccgcg agtccacgaa cgccagccgg tctgcaagcc ggctggccgg ttacgtcagc
    11341 gcgtcgactt cttggcgatg cgttgcggct tcgccactgt cgccttcgtc gccaccgacg
    11401 ccctggccgc gggcttgcgt gcaggcatcg ctcctgacgt cgccgcaacc acaggcgggt
    11461 tcgagccggc atgcgcggcg atgaatgcct tcacctgcgg atacacctgg gtgcgccagc
    11521 ggcggccgct gaagatgccg tagtggccgg caccctcggc ggtcaggtgc ttctggtctt
    11581 ccgcggcgat cccggtgcac agcttgtgcg cggcgcgggt ctggcccgcg ccggaaatgt
    11641 catccagctc gccttcgatg gtgaacagcg cgcagtcgcg gatcttcgag ggatccacgc
    11701 gctcgccggc cacgtcccac agcccgcggg gcaacaggaa ttcctggaac accttctgga
    11761 tggtgtccag gtagtagcgc gcaggcatgt ccagcacggc gttgtattcg tcgtagaact
    11821 tgcggtgcga ctgcgcgtcc tccaggtcgc cgcgcagcag gtcctggtag aagtcccaat
    11881 gcgacgtcgc atgccgttcc gggttcatcg ccacgaaccc caggtgctgc aggaagccgg
    11941 ggtacacctg gcgcccgctg cccggatagt tggcgggcac cgggtggatc acgttgttct
    12001 cgaaccacga caacggttgg cgggtcgcga ggttgttgac cgaggtcggc gattcgcgcg
    12061 ggtcgatcgg tccacccatc atgaccatgg acctgggcaa cgattcgccg cggccggcca
    12121 tcagcgagat cgcggcaagc accggcaccg tcggttggca gacgctgatc acgtgcaggt
    12181 tgtcggtgcc gatatggcgg atgaactcct cgatgtaggc gacgtagtca tccagcccga
    12241 actggccctc cgcggtcggc accatgcgcg cgtcggtcca gtcggtgatg tagaccttgt
    12301 ggtcggccag cagggtgcgc acggtgtcgc gcagcagggt cgcgtggtgg ccggacagcg
    12361 gcgccaccac cagcacggtg gggtcgtcct tcatccgcgc caggccatcg gcctggtcgt
    12421 ggtagcgctt gaagcgcagc agccggcaga acggcttttc cagcgccacc cgctccacca
    12481 ccgggatcgc cagcccctcg acctcgatct ggtggatgcc gaactccggc ttttcgtaat
    12541 ccttgcccag ccggtacatc agctcgaacg ccgccgcggt gcgctgcgca ccgggcactg
    12601 cggtcagcca gctgtccgcc gcggagtaca tcttggcgcc ggcgtcggcg aagtaggtgt
    12661 acggcgcaag gcctgcgcgc cagagttcat gccattggta aagcagcatc ggtcgatgtc
    12721 cgtgcgattg actggcttat gccgcgctgc agcatagccg atcaccggcg accgcccgtg
    12781 acggaggcct cgaatccgga aaaggggccc gttttcccac ctcacctaca ggggcagttc
    12841 cagcgcggca gcctgcgcga cccgcgccgg cgacagccag cagtgcgagc ccagcgggtg
    12901 caggccgacc gcgcgcaggc gcttgcggta gaacccggca ggacgcgcga tgaagccgtg
    12961 gtcgtcgccc actgcctcgt cctcgcgggc gaaggcttcg atgaacgcga ccccgccgca
    13021 caggtcggcc agcgccggca ggccgcgatc gagttcgcgc gtgggcaggt aatgcagcac
    13081 gtcgctgcac accagcaggt cgaccggcgc gcacgggcgc aggaattcga aatcaccgaa
    13141 gcgggccagg tgcaggttgc gcgcgcggcc gtagcggccg gtcgcgtatt cgctggcatc
    13201 gaagccgagg taactcgccc tgggccgcag cttcagcaag ggcgcgcgcc acccgccttc
    13261 gccgcaaccg atgtcgagca cgctgcgcag cggccgttcc aggtggtatt cggcggtggc
    13321 gacggccagc gccaccttgc gcgccagccg cgcgctgcca ccgacttcgc tgccgcggta
    13381 ccagcggtcg aagtaggcgc ggtcgtaggt cttggtcatc gtggcatcct aatggcttcg
    13441 atgccgacgc gagcgagacc atggcctacc tgctgaccaa gaccgcgcac ctggtgttcg
    13501 tgatcgcgtg gatggccggc gtgttctacc tgccgcggat cctggtcaac atcgccgagg
    13561 ccggcggggc ggcggacgtg cgtgcgcggc tgctgctgat ggggcgcagg ctgtaccgct
    13621 tcgggcacgt catgttcgcg ctggccacgc tgctggggct ggtgctgtgg cttcggttcg
    13681 gcgtcaccgg cggctggctg cacgccaagc tgctgctggt cacgctggtg ctgctgcact
    13741 acctggcagc gggacgctgg ctgcgcggcg tcgccaacgg ccggccgctg ccatcgtcca
    13801 ccgccctgcg ctggttcaac gagatcccgg tgctgctgct ggccgcgatc gtctggctgg
    13861 tgctggcgaa gccgttctga tgcgcatggc gtagcgcccg cctgggccgg atgccgcgcg
    13921 accgtcggtc ggtacatcct gtaccgattc gggtcgctgg ccatccctgg cctgcccgcg
    13981 ccagcagccg gcccaggcgg gcgctacgcg cgatcagcat cagcggcctt cagcgcggcc
    14041 tgaactccgc gatctgcggc aactgcaccg gcacgccatt tgcgtcgcac aggtccttcg
    14101 cgcacagcgc agtgcgcagt cgcggggtgg cctgcagcag gatgtggaag atcgcgttct
    14161 gctcgatgct gccgcgcacc gcttcgctgc ccgggccgct ggcccagatg ccgacgtcat
    14221 cgcccccgtg gctttcgctg gccagcggca cggtggcctc ctgcatgaag tccgggtgtt
    14281 cggtgtcgac ctcgcgcagg tccgggcgcc cttccgcggg caggtagtcg cggccggcgt
    14341 ggaagaaccg cttcggcccg gcgggctgcg cgctgctggc gcctgtgtag ccggggccgt
    14401 tggcgtagcc gagggtggtg tagggcaggc cggtggcatc ggccgccagt ccgacttcgg
    14461 cgtcctctgc gccggtgccg cgcaccttgc ccaggatcgg gttgccgcgc accgggtaac
    14521 cggcgaaggt catcgtatgc gcatggtcgg cggtcaccag caccagggtg tcggcggccg
    14581 aggtcgcctc cagcacggca cgcaccgcgt cgctcatcgc caccgtgtcc gccagtgcgc
    14641 ggtaggcgtt gccgtagtga tgggcatggt cgatgcggcc gccctcgacc agcagcacgt
    14701 aaccgcccgg atggcgcgac agccgggtga tcgccgcgcg cgtcatctcc gccagcgagg
    14761 gctcgccggc ggcgtcccgc gtgcggtcgt gctcgaactg catgtggtcg ggctcgaaca
    14821 gcgcgagcag cggcgcgtcg accggcgcgt cctgcagctg acgcaggttc cacacgtagg
    14881 cgccgcccgg gtgccgctgc tgccactcgg cgaccaggtc gcgttcgtcc aggcgcaggc
    14941 cgaccttgtc gtcgtattcc gggtcgcgct gcgataccgg catgaagttc tggcggccgc
    15001 cgcccatcag cacgtcgaaa ccaccggcga acggggtctc caccagctgc cgggcgatgt
    15061 caacgcagcc ggcggcacgc gcggcatcgc tgagctcggt gtcgttttcc cagttgcgct
    15121 cgggcgaatg ggcgaacgtg gcgccggggg tggcatgggt gacgcgggtg ctggtgacca
    15181 cgccggtggc catgccgctg gccgatgcca gttcccacag gctgagcatc ggcgcggcca
    15241 gcgcgccggc gcaatcaccg cgcggggcgg tctggccgat gctcagcacg ccggcgcggg
    15301 tcttcacccc ggtggcgatc gcgctcatcg tgccggccga gtctggcgtt tgcgaattgg
    15361 tgttgtaggt cttgctgagc gcggtggccg ggaaggtctc ccacgacagc cggttctcct
    15421 cgccgctgcc gccgttgcgc tggccctcga acacgcgcgc ggcggtgacc gtggccaggc
    15481 tcatgccatc gccgaggaac acgatcaggt tcttcgcacg gccatccatc gccccgcgcg
    15541 cggcggcctg ggcggcgccg tcacggaacc accacgcggc ggtttccccg ccgggccgtg
    15601 cgatccgcgg gacttcgact tgcagccccg tggcgtgcgc gcccgcgcgc gggtggccgc
    15661 cgctggcggc acaaccgccg gcgacaagcg tggtggcgaa ggcaagggca agaaaggtgg
    15721 ggcgcatgct gggttccgtt ggcgagcgcg gattatgccg gctggacgcg ttggccgcgc
    15781 tgaagtgttc cggcacggcc actgccacgc gcctgcgagg tcgggtatgg tcgcggtttg
    15841 cctgcccggc cgacggggaa gacgcgcgca tgttcgactg gtggttccgc atcaagttct
    15901 ggaaacgcgt ggtcgccggt ttcgtgctcg gcgcgctcgc cggctgggcg ttcgggccgc
    15961 aggcggagac ctggttcggc ccgctcggcg acctctacgt gacactgatc aagatgatcg
    16021 cggtgccatt ggtgttcttc gcggtgatca acgcggtggc ctcgctgggc gggcagaagt
    16081 cgatcgccgc gctggccggg cgcaccttcg cctggttcgc gctgaccgcg gtgctggcgg
    16141 tgggcgtggg cctggccttc ggctggatgt tgcagccggg tgccggcgtc ggcgtgctgc
    16201 aggtggcctc ggactacacg cccaagagcg tccccggcgt gctcgacatg ctcctgaacc
    16261 tggtgccgtc gaacccgttc caggccctgt caggggcggc cagcgcggaa accgccgatg
    16321 gcatggacgt gctggtgccg cgcaacggca ccgtgctgca ggtgatcttc ttcgccggcc
    16381 tggtcggctt cgccatcgtg cgcctgggcg gcagggtcgc cggcatccgc gcgctggccg
    16441 gcgaggccag cgaggtcatg atccaggtca cccgcttcgt gcttgagttc accccgctcg
    16501 gtaccttcgg gctgatcgcg gcgctggtcg gcggctacgg cttcgagcag ctgaagccgc
    16561 tgttcaactt cgtggtcgcg ctgtacgcgg cctgcgcctt ccacatcatc gtggtctatg
    16621 gcggcctgct gctggcgcac gggctcaacc cgctgaagtt cttccgtggc gcggcgccgg
    16681 gcatgcaggt ggcgttcgtc gcttccagca gcttcgccgc gctgccggcg tctttgcgca
    16741 gcgccaccca caacctgggc gtggacaagg actacgcggc gttcgcggtg ccgctgggcg
    16801 ccaccatcaa gatggacggc tgcggcgcga tctacccggc gctggcggcg gtgttcatct
    16861 cgcagtacgc cgggatcgag ctgtcggttt cgcaatacgt ggtgatcgcg ctggcctcgg
    16921 tgctgggcag cttcggcacc gccggggtgc cgggaacggc ggtgatcatg gccacggtgg
    16981 tgctgagcgc cgccggcctg ccgctggagg tgatcggtta cctgtatgcc atcgaccggg
    17041 tgctggacat gatgcggacg atgaccaatg tcaccgggca gatgctggtg ccggtgctgg
    17101 tggcgaagca aagcggcatg ctcgaccgcg cggtctacga cgcggcaccg accgatgtcg
    17161 gactggacga gcgcgggcag gccggctagg cgcgcaatcc tgccccgcgc gcggtgcccc
    17221 cggctcagcg cgcggccggc ttccgcctgg aggccaaccc ggcgccagcc agagccaccg
    17281 ggatcggcag caacaggccc gcgatcgaca tccacagcgg atgcccgggc aattgcacga
    17341 ccatgccgac caccccggcc atcaccagca gcgcgggaac cacggcggcc acccgcggcc
    17401 ggttgccgcg cgcgaccagg gcggcgacga agccgccatc gaatgcaccg gtcagccagg
    17461 ccacgaccac cagcagcatc gcgcccagcg gcatcgactg catgatcatg gccatgtcgg
    17521 cgggcaccat cgggtccagt cccggcggtg gcggatagac ctggtggccg gcgaattcga
    17581 ccaccatgat ggtgaagaag gcgaccagca gcccggccac cgcccccagg atcgtgcgtc
    17641 ccatgtgcat cccctcgtgt gcccggcgca gcatcgcctg cggccgtggg cggcgcaagc
    17701 aacgcccggc atggcgtgcc gcgccgcaaa cgaaaacgcc gcccgcaggc ggcgtcgtgt
    17761 acgcgcgtgc ggggctcagg tgtcggcggc gagccggcgt gcctgcaggt acttctggct
    17821 ccagtagccg ctggcgaggc tggacacggt gacgtcgcgg ccggtgcgcg gcgcgtgcac
    17881 gaacttgcct tcgccgacgt agatgccgac atggtcgacc cggccgcgct tgccgaagaa
    17941 caccaggtcg ccctccacca gggcggcgcg gtcggtgatc gcggtgcctt cgttggccat
    18001 cgcgcgcgag acgcgaggca ggtcgatgcc gatggtgcgg aacacgtagc cgaccaggcc
    18061 gctgcagtcg aagcccgaat ccgggttggt gccgccccag cgatagggcg tgccgagcag
    18121 ggcgaacgcg cgctggagca cggtcctgat gcgggaagtg gtggtgggcg cggcctgcag
    18181 ggcggcgatc tgctcggcca gcggggcatt gtcggggctg gcgaggtcga tgctggcaac
    18241 cgaatccgcg ggtgccaggg tcgcgaccgt ggcctgcgcg gtggtgctgg ccggggcctg
    18301 ttcgggattg cttgcgatcg ccgggacggc gcaggcggtc agcgccatgg caagcaggaa
    18361 acgctgcagc aggcggggaa tttcccgacg ggcggtgcga gcgatggaat ggccgtgggg
    18421 cagttgtgct gtcgtcacgc ggtcatcacg atttggaacc agcggcgacc ttacccggca
    18481 atgcagggtg aagggttcat atttcgttag gcgcacgtaa atgaatgcgc gctgcggtca
    18541 caaaaggcga actcgcaccc attcaggttc gtgattgggc gttgccttgc aagtcattga
    18601 atcaattcag caaccggcgc gcaccgcggt aatggtcccg ccaccaatga ccatccagcc
    18661 ggtccaggcg gaccgtgccg ccgctgctgg gggcatgcac gaagcggcct tcgcccacgt
    18721 agatgccgac atggctgacg ttgccgtcgc tgccgaagaa caccaggtcc ccgccggcca
    18781 gccggtcggt ggcgatcggc gcgtgcggca tcgcaaacag ctcgcgcgag gtgcgcggca
    18841 ggcgcaggtc cagcatgtcc cggtagacgt agttcaccag tccactgcag tcgaacccac
    18901 cctcgggggt gttgccgccg tagcggtagg gcgtgccgac caggctgatc gcgcgcatca
    18961 gcaccgcatt ggcccgggcc ggatcggccg gcgccaccgc cggccacgcc tgcgccgccg
    19021 gtgcggcggg acgcagcgcc tggccgccac cacacgccgc cagcagtgcc atcgatagcg
    19081 ccagcagcgc gttgcgccga taatgcgcgc ccggtcgctg ctggcagtcg gctggttccg
    19141 tcacccgcgc agtgttgccg catcgcatcg agccggcaag cgccaccacc cgagaatccg
    19201 catgaagatc gagaaagacc gcgtcgtccg cttccactac gccgttgccg aggccggcca
    19261 ggaagcggtg gagaattcga aggatggcgg ccagccgctg gcgatcctgt tcggccacgg
    19321 caacatcatc ccgggcctgg aaaaggcgat ggacggccgc gaggccggcg acagcttcaa
    19381 ggccagcgtg gcggccgcgg acgcctacgg cgaacgccgc gagggcctgg gccaacgcat
    19441 cccgaagaag catttcggcg cgcagaagct ggcgccgggc atgcaggtgg tgctgcagac
    19501 caatttcggc ccgcgcgcgg tcaccatcga aaaagtgggg atgagcgtgg tcgacgtcga
    19561 cctcaaccac ccgatggccg gcaaggacct ggagttcgac atcgagatcg tcgaagtgcg
    19621 cgaggcctcc gcggaagagc tggcgcacgg ccatgtgcat ggcgacggcg gccaccacca
    19681 ctgagcgtac gcgccagcgg caatcgcccg gacggcccgc actgcgggcc gttcgcgttt
    19741 gtgtcggggg acggtgaacc agggctccag cccatgactt ccggcgcatg gagaattggt
    19801 gttgtagcga tctaatacag taaaccaagt gcaggaattg accatggccg ccgccgaagc
    19861 gttgcgcccg ctccagctcc atgaacgcat ccagacgctc gacgtcatcc gcggatttgc
    19921 cctgctgggc atcttcctga tgaacgtcga gtggttcaac cggccgatcg ccgacctcgg
    19981 ggccggcgtt cctgcgggat tggccggccc ggacctggtc gtcgcgtggc tgatccacac
    20041 tttcgtcgcc agcaagttct ggccgatgtt ttcgctgctg ttcgggatgg gtttcgcggt
    20101 gatgctgcag cgtgcgcggg ccagcggcag cgcgttcgtg gcgacctatg cgcgtcgcac
    20161 cgcggcgttg gcggcgttcg gccttctgca cggcgtgctg ctgtggccgg gcgacatcct
    20221 gttcgcctac gcggtcaccg cggcggcgct gttgttgacc ctgttcggca cgccgaaaca
    20281 ctggctggcg ctgctgctgc cgttcgcggt gctggccgcg atcccggcca ccagctcggc
    20341 gggggtcgcg atcgtggtgc tgctgttcgc cgcactggtg gcgctgtacc tgcgcgacga
    20401 acgcagcttc cgcggctggc cgcggatcgc gctggtggtc ctggcgatgg cgctgctgat
    20461 gctggtgggc ggcgcggtca cgctggcgaa ggtgggggcg gacaaggggg caccgctggt
    20521 ggtcggcgcg ctgctgctgg tggtggtggc atggctggcc gggcgccatc gcggtcctcc
    20581 ggcgcaacgg gcgctgcgcg cgggggttgc gatcttcctg ctgccgaccc tgatgatgct
    20641 ggcgggcggc ttctacatga tgttcgggcc accggcacag gagggcgcgc gcgccggtgc
    20701 cgagcggccg gttgcggcga caccggcgac tgcaggcaag cagccgcggg cggcggcgcc
    20761 ttcgcccgtc gaggacgccg gggcaaaggc cagggccgcg cagcgcgcgg aagtggcgaa
    20821 gcgcctggcc gagcgcaagg cgcaggcggc ggaagaaacc cggatcatgt ccggcggcac
    20881 ctatgccgag gcggtccgct tccgcgccgg cgcctacctg cgcgacctcg gccagagcgt
    20941 cgggttgtcg gtcatcgcgc tgggcttgtt cctgctcggc ggctggttca tccagtcggg
    21001 gatgatggtg cagccacagc ggcacctgcc ggcattccgc aagctggcgg tgaccgggat
    21061 gctggcgggc ggggcgttgt cgatcgccgc cagcctggtg tcgctgcggc cgctggagcg
    21121 cgccgcggat gcaccgccgg acgcgctgtg gcaactggcc aatgcgctgc acatgatggg
    21181 cgggttgccg ttgatgctgg gctacgtggc ctgcctggtg ctggcgctgc aggccgcgac
    21241 cggccagcgc ctgctgggct ggctggcacc cgcgggacgg atggcgctga ccaactacct
    21301 gatgcaatcg ctgattgcca gcctgttctt ctacggctat ggcctgggcc actggggcat
    21361 gccgcgcgcg cagcaagccg tgttcgtggt ggtggtgttc ggcctgcagg tgttgctgag
    21421 ccactggtgg ctggcgcggt tccgctacgg cccgatcgaa tggctgtggc gctggatgac
    21481 ctacggcacg cggccggcct tgcgattggc ggcgtccgtc gccgcctgag ggtggagacg
    21541 aacctgcaaa cgagaacggc ccgcttgtgc gggccgttcc gtgttgcggt gatgcaatcg
    21601 cttagttgac gttgatcgcc gaccacgccg cggccacggc gttgtactgc gccgagccgg
    21661 cgccatagag cgcggtggcg gcttgcaggg tggccacgcg tgcccccggg taggtggtgc
    21721 tggaggtcat gtagtcgcgg atcgcccggt accagatcgc cgccgccgcc gtgcggccga
    21781 tgccggcgag cgacccgttg ccggtggcgt tcttgctgtc gctgggggcg caggtcctgg
    21841 tcaggctggt ggtgccttcg gccagcaggt agaagaagtg gttggccggg cccgaggagt
    21901 agtgcgggtc cacgccaccc aggccaccgg ccggatagca gtcgtacgag gcgttgccgt
    21961 ccaggttcgg gtcgtacatg tagcgcagcg ggctggagga cagttcctcg ccgatcttgt
    22021 agtcgcccgg atcgttggcg ttgttggcgt agaactccac catcgtgccc atgatgtccg
    22081 agttggcctc gttcaggccg ccggaatcgt tggagtaggc gaggttggct tcggcctggt
    22141 tgacgccgtg gctcatctcg tggccggcca cgtccagcga caccagcggc aggtagccgc
    22201 tgccgccatc gccgtagtac atggcgttgg cgtaccacgc cgcattgatc cagttggtgc
    22261 cgacgtgcac gtagctcttc acgcccttgc cgtcnnnnnn nnnnnnnnnn nnnnnnnnnn
    22321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22381 nnnnnnnnnn nnnnccaccg gtgccgccgc cgcccggctt ggcggtgtgg accatgtccc
    22441 agcggtccag gatcttgccg gtggacgcat ccacgaagta atgcatctcg gtcggggtct
    22501 ggtcgcgctt ggtgccggtg aacaccactt cgtacgccag gcgcgggctg gtgttgcggg
    22561 cgaacaccac cttgcgggct tcctgcacgc cggtgaagtc ggtgccgaac tcggcgccgg
    22621 cggtgatggt ggcctggtcc gcgctgatgc ggccggacag tcccgggcgg ccgctgctgg
    22681 acagggtcat gctggtgccc ttgacctgtc cgttgcgcga atggaccacg aagtcgccgc
    22741 cgatgaccgg catgccgcgg taggtgcggt cgaagcgtac gtgttcggtg ccgtcggcat
    22801 cgacattgac gctgcgggcg atgaaggcat cgccgtcggc tgccttggcg atcgcgccat
    22861 ttgcgcgcag gtggccgagc gcgcgctcgg cggggccggc ggcgaaggcg ccggaggaaa
    22921 gcgcggcaag gacagccgcg gtcagcaagc gggtggaggt gcgcatcggt gttcgaatcc
    22981 ttggcaacgt ggtttgatgc gccctcggaa cagcgttggc gcggcggaca cttggcatac
    23041 tcgccacgcc cggccgcgta cgcggccgga cagcccgaag ctcgactccg cagcgggggc
    23101 tgctgaggcg aaggtgacgg gcgtcacaac gccaagtgtc atcttccatt catgtcatgc
    23161 tgcgacgcac caaatcgttg gaggccttgt cttgactgag ttgcaaccat cgccggccac
    23221 gattcctgct gcaccgcagc aaggaatagg cgattccgac tgcctcgtgg tcggcgccgg
    23281 ggtgtccggg ttggccactg cgcttgcgtt gcttgccgat ggccgcggcg tcctggtggt
    23341 cgatgccggt gagattggcg gcggtgcctc gcacggcaat tgcgggacgc tgacgcccag
    23401 ccacgcgccg ccattggcgg cacccggcac cattccccgc gcgctgcgct ggatgctgac
    23461 cccggacgcg ccgctgtacg ttccgccgcg cttcgacccg gcgctgtggc gctggctgct
    23521 cggtttcgcg atgcgctgca accatcgcga ctggcgcgcg agcacgcgcg ccaagtcggc
    23581 cctgctcaac gactcgcgcc agcgcatcgc cgactgggtg cgcgactacg acctgcagtg
    23641 cgagttcgtg gagagtggcg aggactacgt gttccatgat gcgcgcgcaa tggagcacga
    23701 gctgcgcgat ctccccctgt tgcgcgaatt cgggatcgag gccgaggtca tcgatggccc
    23761 cgcctacgaa cgccaggaac cggcgctgaa gccgggcgtg gcgggtgcga tccgcttcgc
    23821 cggcgatgcc gcgctgcgcc cggaccgcta cgtggcggag ctggcgcgcg cggtgcgcga
    23881 gcgcggcggc accatcatcg aacactgcgc gttgcagtcg ctggaggcgg gcacagatgg
    23941 cgtgcgtgcg gtcactgccc gcggcgcggt gcgcgcccgg gatgcggtga tcgcgaccgg
    24001 ggcgtggtcg ccgacgctcg cccgcgcgat cggcctgccg tggctgcgca aggcgatcca
    24061 gccgggcaag gggtactcga tgacctacag cgcgcccgcg gtggtgccga agcgtccgct
    24121 gacgctgcgc gaagtctcgg tgtgcgtcac cgcctggggc agcggcttcc gcctcggcag
    24181 caccatggaa ttctccggct acgacagcac cctcaacgag cgccggctgg gtgcgctgga
    24241 gcgcggtgcg cgcaagtacc tgcaccatcc ggtcggcccg gcgttgcgcg agcgctggtt
    24301 cggctggcgg ccgatgagcc gcgacgacat cccgctgatc ggtcgcgccc cgggccattc
    24361 gcacctgtgg ctggccaccg gccacggcat gatgggggtg ggcatgagcg ccggcaccgg
    24421 gcagttgctc gccgacatgg tcgcgcagcg ccgcccggcg gtggatccgt ccgcgttcga
    24481 ccccgcgcgc ttcgcctgat ggaacccgtc tacacgttgc atcgtggcag tgcgccgctg
    24541 ctggtcagcc tgccgcacga cggcagcgcg atccccgatg cactggccgc gcgaatgacg
    24601 cccgaggccc ggcgcgcgcc ggacaccgac tggcacgtct cgcggcttta cgccttcgcg
    24661 cgggagctgg gcgcatcgat cctcgtcccg cgccattcgc gctacgtgat cgacctcaac
    24721 cgcggcgagg acgacaccag cctgtaccc
//
LOCUS       DAORUK010000012        24152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_1662_length_24152_cov_19.102212, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000012 DAORUK010000000
VERSION     DAORUK010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 24152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24152
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1662_length_24152_cov_19.102212"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..796
                     /locus_tag="PK818_01635"
     CDS             149..796
                     /locus_tag="PK818_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81468.1"
                     /translation="MGRALLLLATLLAASANAGDRIRVANEGGIRDAWTLPAGAKLAV
                     PAYPAAFAASPAEACVAIGYLIHADGHTSDFALLKAWGAQEPGQRREAYWGEFAGAAA
                     SALAQWRFVPKPEIGSPRPVYTVATFVFASADARETRKRCIIPNLAMHLIELRQGGRA
                     RRRMTDNSLFERLDIDPALEARDRGGEQRRTQDAQPEPLPPPPPSPPSPPPPGEE"
     gene            853..1857
                     /locus_tag="PK818_01640"
     CDS             853..1857
                     /locus_tag="PK818_01640"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class I"
                     /protein_id="HQY81469.1"
                     /translation="MSIEQLAETARAMVAPGKGIIAIDESSATCKKRFDGVGIECTEE
                     TRRAYRELLLTAPNANRYLSGAILFDETIRQSTRDGVPFAKYMLDNGMIPGIKVDKGT
                     HALAGFPGEVITEGLDGLRERLAEYYRLGARFAKWRAVINIGEDIPSGTCIEANSHAL
                     ARYAALCQEQGLVPMVEPEVLMDGDHDIETCYEVTEVVLRSLFDALYNQNVMLEGTIL
                     KASMVVPGKGCEEQASVEEVAESTLMCLKSAVPAILPGIVFLSGGQSDDDATAHLDAM
                     NRMGPNPWPLSFSYGRAMQQAALKLWAQDMAGNYARAQQTVFERAKANGLAALGQWGD
                     "
     gene            2024..2425
                     /locus_tag="PK818_01645"
     CDS             2024..2425
                     /locus_tag="PK818_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81470.1"
                     /translation="MIIPPVPPGQRRKPDPRQLQDAAANAGLVLPDAAMLAFQQGRMI
                     EAIKLIRAANPGLDLARAKAAMERMQAQAHSAAGEAGKGPAGTGKHAMPSPSRRTPTV
                     EMGDRPGQLRWLLLVVALAAAATWIFYAGGS"
     gene            2556..3626
                     /locus_tag="PK818_01650"
     CDS             2556..3626
                     /locus_tag="PK818_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008571895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY81471.1"
                     /translation="MPHLPRALLMCLLLGALLTACGKDEAAKPGGPGGRGGGPVPVVV
                     ETVAAQEWTDALRALGTVHAREAVTITAKVSETVQQVHFESGQQVARGAPLVTLSGQQ
                     QQAALASAEAALGEAEQLFLRQQQLVEQQLIARAALDAQRATRDAARAQVAQVRANLS
                     DRVIRAPFAGVLGIRQVSPGALVTPGTPIATLDDVSRVFVDFPVPETELADVGPGQAL
                     VGRVATYGERSFDGTVATVSTRLDSASRAATVRGDFPNADGALKPGMLVEVSLSRGTR
                     QALVVPEIAVQQIGSETFVWRVKSDDTVEKANVEVGGRVPGKVMLKAGVEAGQRIVTA
                     GMGKLQAGATVAAAGAPPAAAK"
     gene            3630..6764
                     /locus_tag="PK818_01655"
     CDS             3630..6764
                     /locus_tag="PK818_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010370936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY81472.1"
                     /translation="MKLSDLSIKRPVLAVVMSLLLIVLGVMSFLRLTLRELPAIDPPV
                     VSVQVDYPGASASVVETRITQTLEDALAGIEGINTIDSSSQNGASRISIEFYATRDIE
                     AAANDVRNAVSRVADRMPDEADPPEISKVESDSDPIIWLNLRSEKMDLLQLADYAQRY
                     LVDRFSSLEGVAQVRLGGSQRYAMRIWLDGDKLAARGLTAGDVESALRTENVELGAGR
                     IESTDRDFILRVARDYTSAEAFARMPVGKGADGYVVRLGDVARVALESAERRAYYRSN
                     GEPAMGIGIVKTSTANSLDVAQEAKAEAERIKASLPAGTDIYTAFDSTTFISAAVDRV
                     YATLVEAVVLVLVVIWLFLGSARAALIPAVTVPVCVIASFIALHAFGFSINLLTLLAL
                     VLCIGLVVDDAIVVVENIQRRVDLGEPPLVAARRGTAQVAFAVIATTAVLVAVFLPVG
                     FLQGNTGRLFRELSVALAAAVAISAFVALTLTPMMASKLLRPHTGPHAVKENRVSAWL
                     NARFSRMAAGYQRVLDRHVHRTWLFGGLMAAALVALVLLFKLLPSELAPAEDRGSFQM
                     VMEGPEGAGYDYTVRQMQQLEAILAGEVGEGKPILRANPRVPGSFGASEDMHTGRAMI
                     FLQPWDQREQSTAEVAAALEKKFAGMTGVRVRTQVGGGLVRTRGQPFQMVLGGPDYAE
                     IAQWRDTMLAKMAQNPGFIGADSDYKETRPQMRVIVDKARAADLGVSSVAIGTALETM
                     MGGRRATTFVDNGEEYDVVLQAERSGRASTADLGALQVRAKDGSLVPLSNLVTLREIA
                     EPANFNRFNRLRSITLSARLAEGYPLGEAVAWARQAADESLPDHAQVSWKGESRELQE
                     SGGAVLITFALALLIVYLALAAQFESFLHPLVIMLTVPLGVLGALLGLWITGGTVNLF
                     SQIGIVMLVGLAAKNGILIVEFANQLRDAGRNVHEAIVEAAGVRLRPILMTSIATIMG
                     AVPLVVFGGPGSASRATIGIVVISGVAFSTLLSLFVVPAFYSLLARYTRSPEDLAHQL
                     ATLEATTPEAGGHA"
     gene            6757..7611
                     /locus_tag="PK818_01660"
     CDS             6757..7611
                     /locus_tag="PK818_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HQY81473.1"
                     /translation="MHDPGSPWKARQRGHAAPPSARSDRREPQAQADVQPGLQRERRD
                     AELRLHGLNAVRAVFARRPQAIRKLYLAEARIPQLQPLLKWCVANRVGYRVVDEADLQ
                     KLAASAHHEGVVAEVLREAPQSLNAWLRDLPAGPHCALWLDGVGNPHNLGAILRSAAH
                     FGVAAILLPKHSTLALSGAAARVAEGGAEAVPFVRLGRDDNSIAQLHGAGFALAATVV
                     RGGSDLFKADLPQRMVYVLGAEGEGMSPELAAACDMRVSISGSGAVESLNVASACAVL
                     LAAWRSKR"
     gene            complement(7641..9053)
                     /locus_tag="PK818_01665"
     CDS             complement(7641..9053)
                     /locus_tag="PK818_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HQY81474.1"
                     /translation="MRHTALALALTLLVALPLRAQQPTRIDDAAMARAGQLREVALQD
                     DTGWKVVESLTTEVGPRLAGSEADAHAVEWAKARFRALGYDKVWTEPVTFPKWERRAE
                     SARIVGAHAQRLHATALGGSPGGKVEAEVVRFETLDALQKAAPGSLAGKIAFVDYRMT
                     RAKDGSGYGPGSRVRSAGPSAAIRAGAIAYVMRSAGTDSHRNPHTGITRFDEGLQPVP
                     SAALSLPDADQLARLLALGPVRIALDLDCGWNGEYTSQNVIGEISGRAKPDEVVVIGG
                     HLDSWDLGTGAIDDGAGVAITMAAGHLIGRLPKAQRPARTIRVVAFANEEQGLYGGNA
                     YAQKYMGDLRKHQLGAESDFGAGRIHAFTAKVPDYAKDAIARIAQALAPLGIEHRVDG
                     EGCGPDLSPFNDAGMACAGLAQDGTDYFDLHHTPDDTLDKIDPAALAQNVAAYGVFAY
                     LAADADGDFGSAPKVSEPRK"
     gene            complement(9146..9550)
                     /gene="mscL"
                     /locus_tag="PK818_01670"
     CDS             complement(9146..9550)
                     /gene="mscL"
                     /locus_tag="PK818_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012511949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HQY81475.1"
                     /translation="MGMIGEFKDFIARGNVVDLAVGVVIGAAFGKIVTTLVDKVIMPP
                     IGMLVGGVDFSKLALTLKEASVDAAGKEVPAVAIAYGEFFNALIQFVIVAFAIFLVVK
                     AVNRMRKPVEAAPAGPSEDVLLLREIRDSLKR"
     gene            complement(9593..10330)
                     /locus_tag="PK818_01675"
     CDS             complement(9593..10330)
                     /locus_tag="PK818_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQY81476.1"
                     /translation="MGGGNDDLVPAAAAPRVPVHDRDGHRLGGFPVRRIYCVGRNFAD
                     HAREMGASAPASKAGRGTPVFFLKPADAIVIDGVVPYPPGTAELHHEVELVVALGRDA
                     PAGILAPEAAMPLVFAYGIGLDLTRRDLQAAAKAKGLPWDTGKAFDHSAPVSALLPAA
                     GLDGIDDLQLSLDVNGTARQSASLRDLIWPVADILHELSKLYALRAGDLVFMGTPAGV
                     GPLLPGDQYRAALGEVLQLQGRIVAAV"
     gene            complement(10331..10633)
                     /locus_tag="PK818_01680"
     CDS             complement(10331..10633)
                     /locus_tag="PK818_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81477.1"
                     /translation="MSASHRFAFSRNGRFDPFAAPRRPRHRILRVVLGLAGVGLLLAL
                     LFVSVFVGIAMLAAGMLWRLWARRGRPLARTTGATRANSIDGDFRVVGKAQLPMAR"
     gene            10775..11083
                     /locus_tag="PK818_01685"
     CDS             10775..11083
                     /locus_tag="PK818_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HQY81478.1"
                     /translation="MERGGLHEVEAVVDGEAESLILHCDAHGGVRAWLNVCPHAGRRL
                     DWAPGRFLRSKDGLLVCAVHGASFELQHGACVAGPCRGESLRAVAVEVRGGEVFLASP
                     "
     gene            complement(11164..13002)
                     /locus_tag="PK818_01690"
     CDS             complement(11164..13002)
                     /locus_tag="PK818_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQY81479.1"
                     /translation="MDTTLTLTTDMELVLGLVFFTMVMFLFERIRADVVALVVLVVLG
                     LTGLVPREDLFGGFAGNAVMSIIATMILGAGLDRTGALNRLAGWLLRRAHGVESRLLL
                     LTTGAAGLTSSIMQNPSVMSLFLPVASRLSSRTGLTLSRMLLPLAAAIVMGGALTMVG
                     NSPLILLNDLLVAANANMPSGAATLEPLNMFAPMPVGLALLAASLAYFHFFGSKLLRE
                     DGDEAVTPARTQSYFARAYGIDGDVYELTVTADSPLVGMSVGDTEALHGAPLLLALRS
                     GEESRLSPPADARIWVGSVLGMMGAKQQVTDFAQNNFLRLSARLRTFGDLFNPSRAGI
                     SEAVVPPTSAFIGKTSAQLRLRKQLGISLLAINRDKNVLREDVRSTPIRAGDMLVLHS
                     IWADLAQAARGKDLVVVTDYPKDEQRPHKFKLAMAIFAVSMLLALSSKIPVPIALMTG
                     VAGMLVTGVIHIDEAYAAISWKTVFLMACLIPLGWAMDSSGAAAWLAGHSLEQLPDGF
                     PLWLLQILIGLLTTAFSLAISHVGATILMVPIAINIALAAGGDPTAFALIVALSASNN
                     LMTASNPVMSMVTGPANYSSRDLWRVGGPLSLVYLGVIVVVINLLY"
     gene            complement(13027..13761)
                     /gene="trmB"
                     /locus_tag="PK818_01695"
     CDS             complement(13027..13761)
                     /gene="trmB"
                     /locus_tag="PK818_01695"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HQY81480.1"
                     /translation="MTDPFSSDGRKAPPKPFTITEGQRKVRSFVLRQGRFTDAQQRAF
                     DESWPRFGLDYTGQPRDFDAAFGRKAPRILEIGFGNGEALRFSAQRDPGRDHIGIEVH
                     APGVGRLLNALAEDGARNVRLYHHDAVEVLQHEVADGSLDEIRVYFPDPWHKKRHHKR
                     RLLNPEFATLLVRKLAPDGRLHLATDWQDYAGQMWDVLDATAGIANRAGPRGAVARPD
                     WRPQTHFETRGQKLGHGVWDLLYDRC"
     gene            complement(13763..14557)
                     /locus_tag="PK818_01700"
     CDS             complement(13763..14557)
                     /locus_tag="PK818_01700"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HQY81481.1"
                     /translation="MTAISPHDPLVIAGKPYASRLLTGTGKFQDLDETRRATEAAGAQ
                     IVTVAIRRTNIGQDPGEPNLLDVLPPDRYTILPNTAGCYTAEDAVRTCRLARELLDGH
                     RLVKLEVLGDARTLYPDVVATLAAAETLVGDGFDVMVYTSDDPILCKRLEDIGCVAVM
                     PLAAPIGSGLGIQNRYNLLEIVENAKVPIIVDAGVGTASDAAIAMELGCDGVLMNTAI
                     AGARDPVLMAHAMKLAVEAGRAAFKAGRIPRKRFASASSPVDGLIG"
     gene            complement(14660..14860)
                     /gene="thiS"
                     /locus_tag="PK818_01705"
     CDS             complement(14660..14860)
                     /gene="thiS"
                     /locus_tag="PK818_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HQY81482.1"
                     /translation="MRILINGEPRQLPAGSTLVDLLDAEGLGERRVAVEVNGGIVPRS
                     AHAGHQLHAGDRVEIVHALGGG"
     gene            15017..16858
                     /locus_tag="PK818_01710"
     CDS             15017..16858
                     /locus_tag="PK818_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005914545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HQY81483.1"
                     /translation="MAAALALAALPAAVQASDSPFTKTIFFGDSLTDGGYFRPLLIQM
                     VGPNGATIGQFTNNPNYVWSQYLADYYGSNAQAAWTGNMTANPTLASGNNWAVGGART
                     GTSYAGGLGYTPSLAAQYARYLSAGNTVDRNALYTVWGGANDLFAVQANPSQAQQIIG
                     AAVTAQVGLVGALTSAGAQYILVPTLPDLGLTPDSRAGGAAAMAQGTALTSAYNNALF
                     SSLASSNLRVIPLDTFHFLQEVVANPGAYGITNVSGKACAVQPPPAGGSSLFCSPLST
                     VPGGSSYLFADGVHPTAAAHEALADFAISVIEGPRQIALLPHSETVIGRARAQMVDGA
                     IASFGKEDGMRWWADVRGDQQRYEAPYRYDGIGPAASFGIGWTSGNLVYGAFAGYGRQ
                     NNDFGEDRGEFQQTDAGLGGFVGWRGDGLWANAQLAWTDVGFDVDRVVPLGPATRVHS
                     GSPDGDNLSIGASAGWEFEHGALRHGPVLSLLSQQISIDGYAETDPTLSTSLAYPEQD
                     FDSLIGSVGWQASYSINEHLQPYAQITWDREFEEAPEQAFAQAQSMPGSLPYAVPGLE
                     YDDTFGTLVFGLRTKVMGLDANAGASLSVGQKGADDTTFFLSVGSSF"
     gene            16932..17005
                     /locus_tag="PK818_01715"
     tRNA            16932..17005
                     /locus_tag="PK818_01715"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16964..16966,aa:Gly,seq:ccc)
     gene            17083..17541
                     /locus_tag="PK818_01720"
     CDS             17083..17541
                     /locus_tag="PK818_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HQY81484.1"
                     /translation="MAAIAVGWREWAALPELGLPRLRAKVDTGARSSALHVERQWRFV
                     DAGAPWVGFVLLPRAHAGEVEAAAAIVDERLVTDSGGHRTARVFVRTRLRLAGIEREI
                     EMNLSDRCGMRFPLLLGRTALADAFTVDPAGSFLHRRRRGHRPLPPAGDV"
     gene            17564..18481
                     /gene="rimK"
                     /locus_tag="PK818_01725"
     CDS             17564..18481
                     /gene="rimK"
                     /locus_tag="PK818_01725"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HQY81485.1"
                     /translation="MKLAILSRNSKLYSTRRLVEAARERGHSVRVLDPLRCYMRISTE
                     GFEMRYKGAQIAGYHAVVPRIGASVTRYGCAVLRQFELMGSYTPNSAAAIARARDKLR
                     CHQLLAAQGIGLPVTVFGDNPDDTGDLLSMLGPPPHVIKLNEGTQGAGVMLTEKLSAS
                     RGVIETLRGLYAHFLVQEFIAEAKGADLRCFVVGDRVVAAMRRQAPKGDFRSNLHRGG
                     SAKAVRATKAEIATAVRAAKVLGLGVAGVDLIRSKRGPLVLEVNSSPGLEGIEEATGV
                     DVAGCIIEHVAAPAEPPRKHPRNGDLTDS"
     gene            18716..19399
                     /locus_tag="PK818_01730"
     CDS             18716..19399
                     /locus_tag="PK818_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017169438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQY81486.1"
                     /translation="MRILVIEDNPDIAANLGDYLEDQGHVVDFAADGVTGLHLAVVHD
                     FDAIVLDLNLPGMDGLEVCRKLRGEGRKQTPVLMLTARDSLENKLAGFDSGADDYLIK
                     PFALQEVDVRLNALARRGRGPQARILNVGELEFNLDTLEVRREGKLLQLNPTALKILQ
                     SLMEASPAVVTRQDLETRVWGEELPDSDSLRVHIHGLRAMVDKPFATPMIQTRHGIGY
                     RIAPPDAEG"
     gene            19386..20735
                     /locus_tag="PK818_01735"
     CDS             19386..20735
                     /locus_tag="PK818_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQY81487.1"
                     /translation="MPKADASPEPRPAIRRAPRARRRLRTRIILSFALLGLSLTLLLA
                     FATNWVRGRVEEDMITDLMNRNINAYSQRYYNSGGRQIDLPVQQMYGRVVSREKFESL
                     KDDQPDWYRLGDGIHPVTGRNEDGSPFAYKLAVRKTPDTWFFLAYDMQESVRRTEKFE
                     RALYVVVPVFTLFSLLLGWWSASRVMAPVSELARRLRRSGVSSDPEALAPHFARDEVG
                     ELAKALDDYSERLTDVVQRDREFNADVSHELRTPLAVIRGAVELLLSKPDVDDRTRTR
                     LQRIQRAEQQCTDLISALLLLSRNERAVGACDVAKVAQQLLDSHRAQLGGKPLELLLD
                     GAPRLVVDAPESAVSVALGNLIGNAVKYTQSGAVIVRLHDNAVDVIDSGPGLSAEDAA
                     RLFERGYRGTHAGHSQGGGIGLSIVRRLCALYGWDVHVRPGEQRGVVATLRFAQPPA"
     gene            20811..21917
                     /locus_tag="PK818_01740"
     CDS             20811..21917
                     /locus_tag="PK818_01740"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HQY81488.1"
                     /translation="MTAPASAPLAYRVALSDHARSDAQRAAILEGDLGFGRHFTDHMV
                     AIQWDKAQGWHDAQVHAYGPLVLDPAAAVLHYGQEIFEGIKAYRHADGSVWTFRPDAN
                     GQRLQRSAARLTLPQLPVAEFTESLRQLIAVDHAWVPSAPESSLYFRPFMIATEAFLG
                     VRPAQAAAYFVIASPAGAYFARGVAPVSIWLSEDYARAAKGGTGAAKCGGNYAASLLP
                     QQEAHAQGCSQVLFLDPVEGRYLEELGGMNVFLVHRDGRIVTPELSGSILEGITRASV
                     LQLARDRGLVVEERKVSIDEWKQGVAAGDITEVFACGTAAVITPIGELKGRDFAVGDI
                     HAPAGEVTMAIRRELTDIQYGRVPDRHGWMARLR"
     gene            complement(21936..22508)
                     /locus_tag="PK818_01745"
     CDS             complement(21936..22508)
                     /locus_tag="PK818_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HQY81489.1"
                     /translation="MPRLALSFLLLLACALAPDAAVAQVRQCVGADGVLVYSDRKCED
                     IGARERPALPAAEGIGALRPARGGCSRSVQDLVHALGSALQAGDANQVAGVYDWAGMS
                     TANGYRVMERLEAIARRPLVDLQPVYAGGDEYGFDAVEFNADGEVVGTPPRKPRLVGL
                     RVEQTLADGSTPARTVFGLRRHLGCWWIRL"
     gene            complement(22609..23442)
                     /gene="metF"
                     /locus_tag="PK818_01750"
     CDS             complement(22609..23442)
                     /gene="metF"
                     /locus_tag="PK818_01750"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HQY81490.1"
                     /translation="MALRAPVSFEFYPPKTDEQRAQLDRSVQRLKAHAPQFMSVTFGA
                     GGSTLSHTPETVQRLRSEHGVAGVPHITCVGGSREEIRSLLKLYRALGCDRVVALRGD
                     TPSGMARSGDLRYASELVALIRAEHGDHFQVEVAAYPETHPQAEDAHVDLRNFKTKVD
                     AGANGAITQYFYNTDAYFRFVDDARALGIDIPIVPGIMPISNFSQLRRFSEACGAEIP
                     RWIGQRMRAYGDDADGIREFAADCVAGLCRRLLAGGAPSLHFYTLNLAKPTLNVLARL
                     D"
     gene            complement(23512..>24152)
                     /locus_tag="PK818_01755"
     CDS             complement(23512..>24152)
                     /locus_tag="PK818_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HQY81491.1"
                     /translation="YGDVGKGSAASLRAYGARVIVTEIDPICALQAAMEGYEVTTLED
                     TLGRADIYVTTTGNKDIITLAHMQAMKDQAIVCNIGHFDNEIQVDALYASGAVKTNIK
                     PQVDKFTFANGNAIFLLAEGRLVNLGCATGHPSFVMSNSFSNQTLAQIDLWANKDFYD
                     PRVYILPKKLDEEVARLHLEKIGVKLTTLTKQQADYLGVHVDGPFKPEHYRY"
BASE COUNT         3633 a         8251 c         8615 g         3653 t
ORIGIN      
        1 ccctgcgcct gctcgagatc ggcgaggacg gccgtgcgga ggggttgggc gagctctgac
       61 cccgccggct tgacagcgtc cgtgcctggg gattagctgg cgtgcaggca tcgaatgggg
      121 ccatccgatg gacgctcggg gcgggttcgt gggtcgtgct ctcttgctgc tcgccaccct
      181 cctcgcggca agcgcgaatg ccggcgacag gatccgggtc gccaacgagg gcggcatccg
      241 cgacgcctgg acgctgccgg cgggggcgaa actggcggtg ccggcctatc ccgccgcgtt
      301 cgcggccagc ccggccgagg cctgcgtggc gatcggatac ctgatccacg cggatggcca
      361 tacctccgat ttcgcgctgc tgaaggcctg gggcgcgcag gaaccgggcc agcggcgcga
      421 ggcctactgg ggcgaattcg cgggtgccgc ggcatccgcg ctggcgcaat ggcgtttcgt
      481 gccgaagccg gagatcgggt cgccgcgccc ggtctacacg gtggcgacct tcgtgttcgc
      541 ctccgcggat gcacgggaaa cgcgcaagcg ttgcatcatc ccgaaccttg ccatgcacct
      601 catcgaattg cgccagggcg gcagggcgcg gcgcaggatg accgacaaca gcctgttcga
      661 acggctcgac atcgatcccg ccctcgaagc ccgtgaccgg ggcggggagc agcggcgcac
      721 gcaagatgcg caaccagagc cgctgccgcc tccaccgcca tccccgcctt cgccaccccc
      781 gcccggcgag gaatgagtca gcccgggccg gggtctataa ttcgggtttc gccgccagcc
      841 agcaggaacc ccatgagcat cgaacaactc gcagaaaccg cccgtgccat ggtcgccccg
      901 ggcaagggca tcatcgccat cgatgagtcc agcgcgacct gcaagaagcg cttcgacggc
      961 gtcggcatcg agtgcaccga ggaaacccgc cgcgcctacc gcgagttgct gctgaccgcg
     1021 ccgaacgcga accggtacct gtcgggcgcg atcctgttcg acgaaaccat ccgccagtcc
     1081 acccgcgacg gcgtgccgtt cgcgaagtac atgctggaca acggcatgat cccggggatc
     1141 aaggtcgaca agggcaccca cgcactggcc ggcttcccgg gcgaggtgat caccgaaggc
     1201 ctggacggcc tgcgcgagcg gctggccgag tactaccggc tcggcgcgcg cttcgccaag
     1261 tggcgcgcgg tgatcaacat cggcgaggac atcccgtccg gcacctgcat cgaagccaac
     1321 agccacgcgc tggcgcgcta cgccgcgctg tgccaggagc agggcctggt gccgatggtg
     1381 gagccggagg tgctgatgga cggcgaccac gacatcgaga cctgctacga ggtcaccgag
     1441 gtggtgctgc gttcgctgtt cgatgcgctg tacaaccaga acgtgatgct ggaaggcacc
     1501 atcctgaagg cctcgatggt cgtccccggc aagggctgcg aggagcaggc cagcgtcgag
     1561 gaagtggccg agtccaccct gatgtgcctg aagtcggcgg tgccggcgat cctgccgggc
     1621 atcgtgttcc tgtccggcgg gcagagcgac gacgatgcca ccgcgcacct cgatgcgatg
     1681 aaccgcatgg gcccgaaccc gtggccgctg agcttcagct acggccgcgc gatgcagcag
     1741 gccgcgctca agctgtgggc gcaggacatg gccggcaact acgcccgcgc gcagcagacc
     1801 gtgttcgagc gcgccaaggc caatggcctg gcggcgctgg ggcagtgggg tgactgaccg
     1861 cgcttagccg ttgccatccg gcaacggctg cggtcacgaa cgcccgagcg ccacgcgcga
     1921 gggccgggcc aagtccgggg cgccattgat cgaacgaaac gccggcccgg tgccggcgtt
     1981 tcgcatttgt gcggcaggcg ggatccgggc gtaagctgcg gtcatgatca tcccgcccgt
     2041 gccgcccgga caacgccgca aaccggaccc acggcagttg caggacgccg cggcgaacgc
     2101 cgggctggtg ctgcccgacg ccgcgatgct ggcgttccag cagggacgca tgatcgaggc
     2161 aatcaagctg atccgcgccg ccaatcccgg cctggacctg gcgcgggcca aggcggcgat
     2221 ggagcggatg caggcgcagg cgcattccgc cgccggggaa gcggggaaag gtccggccgg
     2281 caccggcaag catgcgatgc cgtcgccatc gcggcggacg ccgacggtgg agatgggcga
     2341 caggcccggc cagttgcgct ggctgctgct ggtggtcgcg ctggccgcgg cggcaacctg
     2401 gatcttctac gccggcggat cctgagccgg cgcgcaacgc gcgggcgcgg catcggttga
     2461 cgccagcggc gcgcagctcc ggcccagccg atggcacacg ggccggcggc ccgcggcagc
     2521 tagaatcacg ggtccccgcc ttgggtttgc cgatgatgcc gcacttgccg cgtgccctcc
     2581 tgatgtgcct gctgcttggc gcgttgctca ccgcctgcgg caaggacgaa gcggcgaagc
     2641 cgggcggccc gggcggacgc ggcggcgggc cggtgccggt ggtggtcgag accgtggctg
     2701 cgcaggaatg gaccgatgca ctgcgagcgc tcggcaccgt gcatgcgcgc gaggcggtga
     2761 cgatcaccgc caaggtcagc gaaaccgtcc agcaggtgca tttcgaaagt ggccaacagg
     2821 tcgcgcgcgg ggcgccgctg gtcaccctca gcggccagca gcaacaggcc gcgttggcat
     2881 ccgccgaggc cgcgctgggg gaagcggagc agctgttcct tcgccagcag cagctggtgg
     2941 agcagcagct gatcgcacgt gccgcgctgg acgcgcaacg cgccacccgc gacgccgcgc
     3001 gcgcgcaggt ggcgcaggtg cgcgccaacc tgtcggaccg ggtgatccgc gcgccgttcg
     3061 ccggcgtgct cgggatccgc caggtcagcc cgggcgcgct ggttaccccg ggcacgccga
     3121 tcgccaccct cgatgacgta tcgcgcgtgt tcgtcgattt cccggtgccg gaaaccgaac
     3181 tggccgatgt cgggccggga caggcgctgg tcggccgggt cgcgacctac ggcgagcgca
     3241 gcttcgacgg caccgtcgcc accgtgtcca cccggctcga cagcgcgtcg cgggcggcca
     3301 ccgtgcgcgg tgacttcccg aatgccgacg gcgcgctcaa gcccggcatg ctggtcgaag
     3361 tcagcctgtc gcgaggcacc cggcaggcgc tggtggtgcc ggagatcgcg gtgcagcaga
     3421 tcggcagcga gaccttcgtg tggcgggtca agtccgacga cacggtggag aaggcgaacg
     3481 tcgaggtcgg tggccgcgtc cccgggaagg tcatgctgaa ggcgggcgtc gaagccggcc
     3541 agcgcatcgt caccgcgggc atgggcaagc tgcaggccgg ggccacggtt gccgcggcag
     3601 gcgccccgcc ggcggcggcg aagtaggcca tgaagctctc cgatctctcg atcaagcggc
     3661 cggtgctggc agtggtgatg agcctgctgc tgatcgtgct gggggtgatg tcgttcctgc
     3721 gcctgaccct gcgcgaattg ccggcgatcg atccgcccgt cgtgtccgtg caggtggact
     3781 acccgggcgc atcggcaagc gtggtcgaaa cccgcatcac ccagaccctg gaggatgcgc
     3841 tggccggcat cgagggcatc aacacgatcg attccagcag ccagaacggc gcctcgcgga
     3901 tcagcatcga gttctacgcc acccgcgaca tcgaggccgc ggccaatgac gtgcgcaacg
     3961 cggtcagccg cgtcgccgac cgcatgccgg acgaggccga cccgcccgag atcagcaagg
     4021 tcgaaagcga ttcagacccg atcatctggc tgaacctgcg ctccgaaaag atggacctgt
     4081 tgcagctggc cgactacgcg cagcgctacc tggtggatcg cttctccagc ctggaagggg
     4141 tggcgcaggt gcgcctcggc ggcagccagc gctatgcgat gcggatctgg ctggacggcg
     4201 acaagctggc ggcgcgcggg ctcaccgcag gcgacgtgga aagcgcgctg cgcacggaga
     4261 acgtggagct cggcgccggc cggatcgaat ccaccgaccg cgacttcatc ctgcgggtgg
     4321 cccgcgacta cacctccgcc gaggccttcg cgcggatgcc ggtcggcaag ggcgcggatg
     4381 gctacgtggt gcggctgggc gacgtggccc gggttgcgct ggagtcggcc gaacgccgcg
     4441 cgtactaccg cagcaacggc gagccggcga tgggcatcgg catcgtcaag acctccaccg
     4501 ccaacagcct ggacgtggcg caggaggcga aggcggaagc cgagcggatc aaggcctcgc
     4561 tgcccgccgg caccgatatc tacaccgcgt tcgacagcac caccttcatc tcggccgcgg
     4621 tcgaccgcgt gtacgcaacc ctggtcgagg cggtggtgct ggtgctggtg gtgatctggc
     4681 tgttcctggg cagcgcgcgc gccgcgctga tccctgcggt gacggtgccg gtgtgcgtga
     4741 tcgcgtcctt catcgcgctg catgcgttcg gattctcgat caacctgctg accctgctcg
     4801 cgctggtgct gtgcatcggg ctggtggtgg acgatgcgat cgtggtggtg gagaacatcc
     4861 agcgccgcgt ggacctgggc gagccgccgc tggtcgcggc caggcgcggc accgcgcagg
     4921 tggcgttcgc ggtgatcgcg accaccgcgg tgctggtggc ggtgttcctg ccggtcggtt
     4981 tcctgcaggg caataccggg cgcctgttcc gcgagctgtc ggtggcgctg gcggcggcgg
     5041 tggcgatctc cgccttcgtc gcgctgacac tgacgccgat gatggcctcc aagctgctgc
     5101 gcccgcacac cggtccgcat gcggtgaagg agaaccgggt cagcgcctgg ctcaacgcgc
     5161 gcttctcgcg catggccgcc ggataccagc gcgtgctgga ccgccacgtg caccggacct
     5221 ggctgttcgg cgggctgatg gcggccgcgc tggtggcgct ggtgctgctg ttcaagctgc
     5281 tgcccagcga actggcgccg gcggaggacc gtggttcgtt ccagatggtg atggaagggc
     5341 cggaaggcgc gggctatgac tacaccgtca ggcagatgca gcagctggag gcgatcctgg
     5401 ccggcgaggt gggcgagggc aagccgatcc tgcgcgccaa cccgcgcgtg cccggcagct
     5461 tcggcgccag cgaggacatg cacaccggcc gcgcgatgat cttcctgcag ccgtgggacc
     5521 agcgcgagca gagcaccgcc gaagtcgccg cggcgctgga gaagaaattc gccgggatga
     5581 ccggggtgcg cgtgcgaacc caggtcggcg gcggcctggt gcggacccgt gggcagccgt
     5641 tccagatggt gctgggcggg ccggactatg cggaaatcgc gcagtggcgg gacaccatgc
     5701 tggcgaagat ggcgcagaac cccggcttca tcggcgcgga ttccgattac aaggaaaccc
     5761 gcccgcagat gcgggtgatc gtggacaagg cgcgcgcggc cgaccttggc gtttcctcgg
     5821 tggcgatcgg caccgcgctg gaaaccatga tgggcgggcg ccgcgccacc accttcgtcg
     5881 acaacggcga ggaatacgac gtggtgctgc aagccgagcg cagcggacgc gcgtccaccg
     5941 ccgacctcgg tgcgctgcag gtgcgcgcga aggacggctc gctggtgccg ctgtcgaacc
     6001 tggtgacgct gagggaaatc gccgaaccgg ccaacttcaa ccgcttcaac cgcctgcgtt
     6061 cgatcaccct cagcgcgcgc ctcgccgagg gttacccgct gggcgaggcg gtggcgtggg
     6121 cgcggcaggc cgccgacgag agcctgccgg accacgcgca ggtcagctgg aagggcgagt
     6181 cgcgcgagct gcaggagtcc ggcggcgcgg tgctcatcac cttcgcgctg gcgctgctga
     6241 tcgtgtacct ggcgctggcc gcgcagttcg aaagcttcct gcatccgctg gtgatcatgc
     6301 tcaccgtgcc gctgggcgtg ctcggcgcgc tgctgggcct gtggatcacc ggcggcaccg
     6361 tcaacctgtt ctcgcagatc gggatcgtca tgctggtggg gctggcggcg aagaacggga
     6421 tcctgatcgt ggagttcgcc aaccagctgc gcgacgccgg gcgcaacgtg cacgaggcga
     6481 tcgtcgaagc cgccggcgtg cgcctgcgcc cgatcctgat gacctcgatc gccaccatca
     6541 tgggcgcggt gccgctggtg gtgttcggcg gccccggttc ggccagccgc gcgaccatcg
     6601 gcatcgtggt gatctccggg gtcgcgttct cgaccctgct gtcgctgttc gtggtgccgg
     6661 cgttctattc gctgctggcc cgttacacgc gctcgccgga ggacctggcg caccagctgg
     6721 ctacactgga ggccacgacg ccggaggcag gcggccatgc atgatccggg ttccccatgg
     6781 aaggcgcgcc agcgcggcca cgccgcgccc ccctccgcgc gcagcgatcg gcgcgaaccg
     6841 caggcacagg cggacgtcca gcccggcctg caacgcgagc gccgcgatgc cgagctgcgc
     6901 ctgcatggcc tgaacgcggt gcgtgcggtg ttcgcacggc gcccgcaggc gatccgcaag
     6961 ctgtacctgg ccgaggcgcg gatcccgcag ttgcagccgc tgctgaagtg gtgcgtggcc
     7021 aaccgggtcg gctaccgggt ggtggacgag gccgacctgc agaagctcgc cgccagcgcg
     7081 caccacgaag gcgtggtggc cgaggtcctg cgcgaggcac cgcagtcgtt gaacgcgtgg
     7141 ttgcgcgacc tgcccgccgg cccgcactgc gcgctgtggc tggacggtgt gggcaatccg
     7201 cacaacctcg gcgcgatcct gcgcagcgcc gcgcatttcg gggtggccgc gatcctgctg
     7261 cccaagcatt ccaccctcgc cctgtccggc gccgccgcgc gggtggccga aggcggcgcc
     7321 gaggcggtgc cgttcgtgcg acttggccgc gatgacaaca gcatcgcgca gctgcatggc
     7381 gcgggcttcg cgctggccgc caccgtggtg cgcggcggca gcgacctgtt caaggccgat
     7441 ctgccgcagc ggatggtcta cgtgctcggt gccgaaggcg agggcatgtc gcccgaactt
     7501 gccgcagcct gcgacatgcg cgtgtcgatc tccggcagcg gcgcggtgga gagcctcaac
     7561 gtggccagtg cctgcgccgt gttgctggcg gcttggcgca gcaagcgcta ggaactgcgc
     7621 ctcgcttcaa ccaaacgtga ctatttccgt ggctcggaca ccttcggcgc gctgccgaaa
     7681 tcgccgtcgg catccgcggc gaggtaggca aacacgccat aggcggccac gttctgcgcc
     7741 agcgcggccg ggtcgatctt gtccagggtg tcgtccggcg tgtggtgcag gtcgaagtag
     7801 tcggtgccgt cctgcgcaag cccggcgcag gccatgccgg cgtcattgaa cggcgacagg
     7861 tccggcccgc agccttcgcc atcgacccga tgttcgatgc ccagcggcgc cagcgcctgc
     7921 gcgatgcgcg cgatcgcgtc cttcgcgtag tccggcacct tcgccgtgaa cgcatggatg
     7981 cggccggcgc cgaaatcgct ttccgcgccc agctggtgct tgcgcaggtc gcccatgtac
     8041 ttctgcgcat aggcgttgcc gccgtacagg ccctgctcct cgttggcgaa cgcaaccacc
     8101 cggatcgtgc gcgccgggcg ctgcgccttc ggcagcctgc cgatcaggtg gccggccgcc
     8161 atcgtgatcg caacgccggc gccatcgtcg atcgcacccg tgcccaggtc ccacgaatcc
     8221 aggtggccgc cgatcaccac cacctcgtcg ggcttcgcac gcccgctgat ctcgccgatc
     8281 acgttctgcg aggtgtattc gccgttccag ccacagtcca ggtcgagcgc gatccggacc
     8341 ggacccagtg ccagcagccg cgccagctgg tcggcatcgg gcagcgacag cgccgccgag
     8401 gggaccggct gcaggccttc atcgaagcgg gtgatgccgg tgtgcgggtt gcggtgcgag
     8461 tcggtgccgg ccgagcgcat cacgtaggca atggcgccgg cgcggattgc cgccgaggga
     8521 ccggcgctgc gcacgcgcga gccggggccg tagccactgc cgtcctttgc gcgggtcatc
     8581 cggtagtcga cgaacgcgat cttgcccgcc agcgaccccg gcgcggcctt ctgcagcgcg
     8641 tccagggtct cgaaccgcac cacctctgcc tcgaccttgc cgcccgggct gccacccagc
     8701 gccgtggcat gcagccgctg cgcatgcgca ccgacgatgc gtgcggattc ggcgcggcgt
     8761 tcccacttcg ggaaggtcac cggctcggtc cagaccttgt cgtagcccag cgccctgaac
     8821 ctggccttcg cccattccac cgcgtgcgcg tcggcctcgc tgccggccag gcgcgggccg
     8881 acttcggtgg tcagcgactc caccaccttc cagccggtgt catcctgcag ggcgacctcg
     8941 cgcagctgcc cggcgcgcgc catcgcggca tcgtcgatgc gggtcggttg ttgcgcccgc
     9001 agcggcagcg ccaccagcag ggtcagtgcg agggcgagtg cggtatggcg catcgagcga
     9061 actccgggtg ccggaaacgg cgaagccgcg agcctagcag cccgcggctt cgtcgatcac
     9121 gtgccggaag tcgccggcgg gcgcgtcagc gcttgagcga atcgcggatc tcgcgcagca
     9181 gcagcacgtc ctcgctcggg ccggccggtg cggcttccac cggcttgcgc atgcggttga
     9241 ccgccttgac caccaggaag atggcgaatg cgacgatcac gaactggatg agcgcgttga
     9301 agaattcgcc gtaggcgatc gccaccgccg gcacctcctt gccggccgca tcgacgctgg
     9361 cttccttcag ggtcagggcg agcttcgaga aatcgacgcc accgaccagc atgccgatcg
     9421 gcggcatgat caccttgtcc accagggtcg tcacgatctt gccgaaggcg gcaccgatca
     9481 ccacgccgac cgcgaggtcg accacgttgc ccctggcgat gaaatccttg aactcgccga
     9541 tcattcccat gcgtcagctc ccgttggtcc gtaaattccc cgaggcgaca gcctacaccg
     9601 ccgccacgat cctgccctgc agttgcagca cctcgccgag cgcggcccgg tactggtccc
     9661 ccggcagcaa tgggccaacg ccggccggcg tgcccatgaa caccaggtcg cccgcacgca
     9721 gggcgtacag cttcgacagc tcgtgcagga tgtcggccac cggccagatc aggtcgcgca
     9781 ggctcgcgga ttggcgcgcg gttccgttga cgtccagcga cagctgcagg tcatcgatgc
     9841 cgtccagccc ggccgccggc agcagggcac tcaccggcgc cgagtgatcg aacgccttgc
     9901 cggtatccca tggcaggccc ttggccttgg ccgccgcctg caggtcgcgc cgggtcaggt
     9961 ccaggccgat gccgtaagcg aacaccagcg gcatcgccgc ttccggcgcc aggatcccgg
    10021 ccggcgcatc gcggccaagc gcgaccacca gttccacttc gtggtgcaac tcggcggtgc
    10081 cgggcggata cggcaccacg ccatcgatga cgatcgcatc ggcaggcttc aggaagaaca
    10141 ccggggtgcc gcgcccggcc ttcgacgcgg gcgcgctggc gcccatttcg cgcgcgtgat
    10201 cggcgaagtt gcggcccacg cagtagatgc ggcggaccgg gaacccgccg agccggtgac
    10261 cgtcgcggtc atgcaccgga acgcggggtg cggcggcggc cggcaccagg tcgtcgttgc
    10321 cgccgcccac tcagcgcgcc atcggcagct gcgccttgcc gaccacccgg aagtcgccgt
    10381 cgatgctgtt ggcgcgggtg gcgccggtgg tgcgcgcgag cggccggccg cgccgcgccc
    10441 acaggcgcca gagcatgccg gcggcaagca tcgcgatgcc gacgaacacg ctgacgaaca
    10501 gcagcgccag cagcaggccg acgccggcca ggcccagcac cacgcgcagg atccggtggc
    10561 gcggacggcg cggggccgcg aacggatcga agcggccgtt gcgcgagaaa gcgaagcgat
    10621 gggatgcgga catcggttgc ggattcctgc ggcgcgcatg cgccatgaca caattgcggc
    10681 aagtatccgg gtagcgcgaa tccgagttgt gagcagttcg ttaaatcgcg atggcgtgga
    10741 tcccgtggcc ggcgcgctgc tgccgctggc gtcgatcgag cgcggcggcc tgcatgaggt
    10801 cgaggcggtg gtggacggcg aggcggagtc gctgatcctg cattgcgatg cgcacggcgg
    10861 cgttcgcgca tggttgaacg tgtgcccgca tgccgggcgc cggctggact gggcgccggg
    10921 caggttcctg cgcagcaagg atggcctgct ggtgtgcgcg gtccacggcg ccagcttcga
    10981 actgcagcat ggcgcctgcg tggccgggcc gtgccgcggc gaatcgctgc gtgcggtcgc
    11041 ggtcgaggtg cgtggcggcg aggtgttcct cgcatcgccg taggagcgcg gcttgcgccg
    11101 cgggcgcgcg gtggcggttg cgcggggcaa ccccccgcac ctgcattgcc cggtgcaatc
    11161 cgctcagtac agcaggttga tcaccaccac gatcaccccg aggtagacca gcgacagcgg
    11221 tccgccgacc cgccacaggt cgcggctgga ataattggcc gggccggtga ccatcgacat
    11281 caccgggttc gatgcggtca tcaggttgtt ggaggccgac agcgcaacga tcagggcgaa
    11341 tgcagtggga tcgccgccgg cggccagcgc gatgttgatc gcgatcggga ccatcaggat
    11401 ggtcgcgccg acatggctga tcgccagcga gaacgcggtg gtcagcaggc cgatcagtat
    11461 ttgcagcagc cacagcggga agccgtccgg cagctgttcc aggctgtgcc cggcaagcca
    11521 tgccgcggcg ccgctggagt ccatcgccca gcccagcggg atcaggcagg ccatcaggaa
    11581 cacggtcttc cagctgatcg cggcgtacgc ctcgtcgatg tggatcacgc cggtcaccag
    11641 catgccggcg acgccggtca tcagcgcgat cggcaccggg atcttcgagc tcagcgccag
    11701 cagcatcgac accgcgaaga tcgccatcgc cagcttgaac ttgtgcgggc gctgctcgtc
    11761 cttcgggtag tcggtgacca ccaccaggtc cttgccgcgc gccgcctgcg caaggtcggc
    11821 ccagatgctg tgcagcacca gcatgtcgcc ggcgcggatc ggggtgctgc gcacgtcctc
    11881 gcgcagcacg ttcttgtcgc ggttgatcgc cagcaggctg atccccagct gcttgcgcag
    11941 gcgcagctgc gccgaggtct tgccgatgaa ggcggaggtc ggcggcacca ccgcctcgga
    12001 aatgccggcg cggctggggt tgaacaggtc gccgaaggtg cgcagccgcg ccgacagccg
    12061 caggaagttg ttctgcgcga aatcggtcac ctgctgcttc gcgcccatca tgccgagcac
    12121 gctgccgacc cagatccgcg catccgccgg cggcgacagc cgcgattcct cgccgctgcg
    12181 cagcgccagc agcaacggcg cgccgtgcag ggcctcggta tcgcccacgc tcatgccgac
    12241 cagcgggctg tcggcggtga ccgtgagttc gtagacgtcg ccgtcgatgc cgtaggcgcg
    12301 ggcgaagtag ctttgcgtgc gcgccggggt gaccgcttcg tcgccgtcct cgcgcagcag
    12361 cttgctgccg aagaagtgga agtacgccag cgaggcggcg agcagggcca ggccgaccgg
    12421 catcggcgcg aacatgttca gcggttccag ggtggccgcg ccggacggca tgttggcgtt
    12481 ggccgcgacc agcaggtcgt tgagcaggat cagcggcgaa ttgccgacca tggtcaacgc
    12541 gccgcccatc acgatcgccg ccgccagcgg cagcagcatc cgcgacaggg tgaggccggt
    12601 gcgcgacgac agccgcgagg ccaccggcag gaacagcgac atcaccgacg ggttctgcat
    12661 gatcgagctg gtcaggccgg cggcgccggt ggtcagcagc agcaggcgcg attcaacccc
    12721 gtgcgcgcgc cgcaacagcc agccggcgag gcggttcagg gcgccggtgc ggtccagtcc
    12781 cgcgcccagg atcatggtgg cgatgatgct catcaccgcg ttgccggcga agccgccgaa
    12841 caggtcctcg cgcggcacca gcccggtcag gcccagcacc accagcacca ccagcgcgac
    12901 cacgtcggcg cggatgcgct cgaacaggaa catcaccatc gtgaagaaca ccaggccgag
    12961 caccagctcc atgtcggtgg tgagcgtcag cgtggtgtcc atttcgcgcc gtgcgttcct
    13021 tcggtgtcag cagcggtcgt acagcaggtc ccacacgccg tggccgagct tctggccgcg
    13081 ggtttcgaaa tgcgtctgcg ggcgccagtc cggccgcgcc accgcgccgc gcgggcccgc
    13141 acgattggcg atgccggcgg tcgcatccag cacgtcccac atctgccctg catagtcctg
    13201 ccaatcggtg gcgaggtgca agcggccatc cggcgccagc ttgcgcacca gcagcgtggc
    13261 gaactccgga ttcagcagcc gccgcttgtg gtggcgcttc ttgtgccacg ggtccgggaa
    13321 gtagacgcgg atctcgtcca gcgaaccgtc ggcgacttcg tgctgcagca cttccaccgc
    13381 atcgtggtgg tagagccgca cgttgcgcgc gccgtcctcg gccagcgcgt tcagcaggcg
    13441 gccgacgccg ggcgcgtgca cctcgatgcc gatgtggtcg cggccgggat cgcgttgcgc
    13501 ggagaagcgc agggcctcgc cattgccgaa gccgatctcg aggatgcgcg gcgccttgcg
    13561 cccgaacgcg gcgtcgaaat cccgtggctg gcccgtgtag tcgaggccga agcgcggcca
    13621 cgattcgtcg aacgcgcgct gctgggcgtc ggtgaagcgg ccctggcgca gcacgaagct
    13681 gcgcaccttg cgctggcctt cggtgatcgt gaacggcttc ggcggggcct tgcgtccgtc
    13741 gctcgaaaag gggtcggtca tctcagccga tcagcccgtc gaccggcgag gacgcggacg
    13801 cgaagcgctt gcgcgggatg cgcccggcct tgaacgcggc gcgtccggct tccaccgcca
    13861 gtttcatcgc atgcgccatc agcaccgggt cgcgggcacc ggcgatcgcg gtgttcatca
    13921 gcaccccgtc gcagccgagt tccatcgcga tcgcggcgtc ggaggcggta ccgacgccgg
    13981 catcgacgat gatcggcacc ttcgcgttct cgacgatctc cagcaggttg tagcggttct
    14041 ggatgcccag cccggagccg atcggcgccg ccagcggcat caccgccacg cagccgatgt
    14101 cctccagccg cttgcacagg atcggatcgt cgctggtgta gaccatcacg tcgaagccgt
    14161 cgccgaccag ggtttccgcc gcggccaggg tcgcgaccac gtcggggtac agggtcctgg
    14221 catcgcccag cacctcgagc ttgaccaggc ggtggccatc cagcagttcg cgcgccagcc
    14281 ggcatgtgcg caccgcgtcc tcggcggtgt agcaaccggc ggtgttgggc aggatcgtgt
    14341 agcggtccgg cggcagcacg tcgagcaggt tcggctcacc cgggtcctgg ccgatgttcg
    14401 tgcggcggat cgcgacggtg acgatctgcg cgccggcggc ttcggtggcg cggcgggttt
    14461 cgtccaggtc ctggaacttg ccggtgccgg taagcaggcg cgaggcgtag ggcttgccgg
    14521 cgatcaccag cgggtcgtgc ggggagatgg cagtcatcgg cggattatcg ccgatgcaac
    14581 ggcgcatgtt cgcccgcccg atggccgtcg ccgggaccgg gccgccgcgg cgcgaaccag
    14641 cgccacgccc gccctcggct cagcctccgc ccagtgcgtg cacgatctcc acccggtcgc
    14701 ccgcgtgcag ctggtgcccg gcatgcgcgc tgcgcgggac gatgccgcca ttcacttcga
    14761 ccgccacccg gcgttcgccc aggccctccg cgtcgagcag gtcgacgagg gtgctgcctg
    14821 cggggagttg gcgcggctcg ccattgatga ggattcgcat gggcggattt tcgcctgttt
    14881 cggcgcgcgc cgctggctgc ggcgctgcga cttgtgaccg ttacgatttc gtgaaaccat
    14941 cggcaccatt cgctgtcgta ctgtcggcgc acacccagag gactccacga tgaaagccaa
    15001 gttccgtcat tccctgctgg ccgctgcgtt ggcactggcc gccctgccgg ccgccgtgca
    15061 ggcatccgac tcgccgttca ccaagaccat cttcttcggt gacagcctca ccgatggcgg
    15121 ctacttccgt ccgctgctga tccagatggt cgggccgaac ggcgccacca tcggccagtt
    15181 caccaacaac ccgaactacg tctggtcgca gtacctggcc gactactacg gcagcaatgc
    15241 ccaggctgcg tggaccggca acatgaccgc caacccgacc cttgccagtg gcaacaactg
    15301 ggccgttggc ggtgcgcgca ccggcaccag ctacgcgggc gggcttggct acacgccgtc
    15361 gctggccgcc cagtacgcgc gctacctgag tgccggcaat acggtggacc ggaacgcgct
    15421 gtacaccgtg tggggtggtg ccaacgacct gttcgcagtg caggcgaacc cgtcgcaggc
    15481 ccagcagatc atcggtgccg cggtcaccgc gcaggttggc ctggtgggtg cactgaccag
    15541 cgcgggcgcg cagtacatcc tggtgccaac cttgcccgac ctcggcctga cgcccgactc
    15601 gcgtgccggt ggggcggccg ccatggcgca gggcaccgcg ctgacgagcg cctacaacaa
    15661 cgcgctgttc agcagcctgg cttcgtcgaa cctgcgggtc attccactgg atacgttcca
    15721 tttcctgcag gaagtggtgg cgaacccggg cgcgtacggc atcaccaacg tcagcggcaa
    15781 ggcctgcgcc gtccagccgc cgccggccgg cgggtcctcg ctgttctgca gcccgttgtc
    15841 gaccgtgcct ggcggctcca gctacctgtt cgctgacggc gtgcatccca ccgccgccgc
    15901 gcacgaggcg ctggccgact tcgccatttc ggtgatcgag ggcccgcgcc agatcgccct
    15961 gctgccccat tccgaaaccg tcatcggccg tgcccgcgcg cagatggtgg atggcgcgat
    16021 cgccagcttc ggcaaggaag acggcatgcg ctggtgggcc gacgtgcgcg gcgaccagca
    16081 gcgctacgag gcgccgtacc gctatgacgg catcggcccg gccgcctcgt tcgggatcgg
    16141 ctggaccagc ggcaaccttg tctatggcgc gttcgccggc tatggccgcc agaacaacga
    16201 cttcggcgaa gatcgcggcg agttccagca gaccgatgcc ggccttggcg gcttcgtcgg
    16261 ctggcgcggc gacgggctgt gggcgaacgc ccagctggcc tggaccgatg tcggcttcga
    16321 tgtcgaccgc gtcgtcccgc tcggccccgc gacccgcgtg cacagtggct cgccggacgg
    16381 cgacaacctg agcatcggcg ccagcgccgg ctgggaattc gagcacggcg ccctgcgcca
    16441 cggcccggtg ctgtcgctgc tgtcgcagca gatctcgatc gacggctacg ccgagaccga
    16501 cccgaccctg tccacctcgc tggcgtatcc ggagcaggac ttcgactcgc tgatcggcag
    16561 cgtcggctgg caggccagct actcgatcaa tgagcacctg cagccgtatg cgcagatcac
    16621 ctgggaccgc gagttcgagg aggcgccgga gcaggccttc gcgcaggcgc agtcgatgcc
    16681 gggcagcctg ccgtacgcgg tgccgggcct ggaatacgac gacaccttcg gcacgctcgt
    16741 gttcggcttg cggaccaaag tgatgggcct ggacgccaat gccggcgcca gcctgagcgt
    16801 gggccagaag ggtgccgatg acacgacctt cttcctgagc gtgggcagca gcttctgatc
    16861 gatccggcgg tgcggcgcgc ccctgcgcgc gttgcatcgc ccgcgccacg cggactagac
    16921 ttggcgcgat cgcgggtgta gctcaatggc agagctgttg cttcccaagc aactgacgag
    16981 ggttcgattc ccttcacccg ctccaactcc ggattggtga ggcaagccaa cggcggtatt
    17041 ccaacggaat gccgccgttt cgttttgcag gcgagggcac ggatggcggc gatcgcggtc
    17101 ggttggcgtg aatgggcggc cctgcccgaa ctgggcctgc cgcgcctgcg cgccaaggtc
    17161 gacaccggtg cgcgcagctc ggccctgcac gtggagcggc aatggcggtt cgtcgatgcc
    17221 ggcgcgccgt gggtgggctt cgtgctgttg ccgagggcgc atgccggcga ggtggaggcc
    17281 gcggcggcga tcgtggacga acgcctggtg accgattccg gcgggcaccg gaccgcgcgc
    17341 gtgttcgtgc ggacccggct gcgcttggcc gggatcgaac gcgaaattga aatgaacctg
    17401 tcggatcgtt gcgggatgcg gttcccgctg ctgctcgggc gcaccgcgct ggcggacgcg
    17461 ttcacggtcg atcccgcggg ctcgttcctg caccgccgcc gtcgcgggca ccggccgctg
    17521 ccgcccgcgg gcgacgtttg aagcaccaca cgagaaggcc tgcatgaaac tggcgatcct
    17581 gtcccgcaac agcaagctgt attcgaccag gcgcctggtc gaagccgcgc gcgagcgtgg
    17641 ccacagcgtg cgcgtgctgg atccgctgcg ctgctacatg cggatcagca ccgaaggctt
    17701 cgagatgcgc tacaagggcg cgcagatcgc cggctaccac gcggtggtgc cgcgcatcgg
    17761 tgcctcggtc acccgctacg gctgcgcggt gctgcgccag ttcgaactga tgggcagcta
    17821 taccccgaac agcgcggcgg cgatcgcgcg cgcgcgcgac aagctgcgct gccaccagtt
    17881 gctggccgcg caggggatcg gcctgccggt gaccgtgttc ggcgacaacc cggacgacac
    17941 cggcgacctg ctgtcgatgc tcggcccgcc gccgcacgtg atcaagctca acgagggcac
    18001 ccagggcgcc ggggtgatgc tgaccgagaa actgtcggca tcgcgcgggg tgatcgagac
    18061 cctgcgcggc ctgtacgcgc atttcctggt gcaggaattc atcgccgagg cgaagggcgc
    18121 ggacctgcgc tgcttcgtgg tcggcgacag ggtggtcgcg gcgatgcgca ggcaggcgcc
    18181 gaagggcgat ttccgctcca acctgcatcg cggcggcagc gcgaaggcgg tgcgtgcgac
    18241 caaggccgag atcgcgaccg cggtgcgcgc ggccaaggtg ctcgggctgg gcgtggccgg
    18301 cgttgacctg atccgcagca agcgcggccc gctggtgctg gaggtgaact cctcgccggg
    18361 gctggagggc atcgaggagg cgaccggggt ggatgtcgcc ggctgcatca tcgaacacgt
    18421 ggcggcgccg gccgagccgc cgcggaaaca tccgcgcaac ggggatttga cggattctta
    18481 atcgatgcgg gtgtaacgtt cccccgcggt cccgcttcac ttcccccggg gtgggccgcg
    18541 cagggcagtt gtgaaccttg tcagtcgttt cgggcccagg cgccacgcgc ctgggccttc
    18601 gtttgtccgg cacccggggc ggccatcccg gcgccgctat gccagaatcc gggaaccccg
    18661 gcaacgggat ccggtcatag ccaagcccgt ggccctcgcc gcaaggaatg aacgcatgcg
    18721 catcctcgtc atcgaagaca atcccgacat cgccgccaac ctcggcgact acctcgagga
    18781 ccagggccat gtcgtggatt tcgccgccga tggcgtgacc ggcctgcacc tggcggtggt
    18841 gcacgatttc gacgcgatcg tgctcgacct caacctgccc gggatggacg ggctggaagt
    18901 ctgccgcaag ctgcgcggcg agggccgcaa gcagaccccg gtgctgatgc tgaccgcgcg
    18961 cgattcgctg gagaacaagc tggccgggtt cgactccggt gccgacgatt acctgatcaa
    19021 gccgttcgcg ctgcaggaag tggacgtgcg gttgaatgcg ctggcccgcc gcggccgcgg
    19081 cccgcaggcg cggatcctca acgtgggcga actcgaattc aacctcgaca ccctcgaggt
    19141 gcggcgcgaa ggcaagttgc tgcaactcaa cccgaccgcg ctgaagatcc tgcagtcgct
    19201 gatggaggcc tctcccgcgg tggtcacccg gcaggacctg gaaacccggg tgtggggcga
    19261 ggaattgccc gattcggatt cgctgcgggt gcacatccac ggcctgcgcg cgatggtgga
    19321 caagccgttc gccaccccga tgatccagac ccggcacggc atcggctacc ggatcgcacc
    19381 gcccgatgcc gaaggctgac gcctcgcccg agccgcgccc cgcgatccgc cgcgcgccgc
    19441 gtgcgcgaag gcggctgcgt acccgcatca tcctgtcgtt cgccctgctg ggcctgagcc
    19501 tgaccctgct gctcgcgttc gcgaccaact gggtccgcgg ccgggtcgag gaggacatga
    19561 tcaccgacct gatgaaccgc aacatcaatg cgtactcgca gcggtactac aacagcggtg
    19621 gccggcagat cgacctgccg gtgcagcaga tgtacgggcg cgtggtgtcg cgcgagaagt
    19681 tcgaatcgct gaaggacgac cagccggact ggtacaggct gggcgacggc atccatccgg
    19741 tgaccggccg caacgaggat ggctcgccgt tcgcctacaa actggcggtg cgcaagacgc
    19801 cggatacctg gttcttcctt gcctacgaca tgcaggaaag cgtgcgccgc accgagaaat
    19861 tcgagcgggc gctgtacgtc gtggtgccgg tgttcaccct gttctcgctg ttgctgggct
    19921 ggtggtccgc ctcgcgggtg atggcgccgg tttcggaact ggcgcgacgg ttgcgccgca
    19981 gcggcgtcag ttccgacccc gaagcgctgg cgccgcattt cgcgcgcgac gaggtgggcg
    20041 agctggcgaa ggcgctggac gattattcgg aacgcctgac cgacgtggtg cagcgcgacc
    20101 gcgagttcaa cgccgacgtc agccatgagc tgcgcacccc gctggcggtg atccgcggcg
    20161 cggtcgaact gctgctgtcg aagccggacg tggacgaccg cacgcggacc cggctgcagc
    20221 gcatccagcg cgccgaacag caatgcaccg acctgatcag cgccctgctg ctgctctcgc
    20281 gcaacgagcg cgcggtcggt gcctgcgacg tggcgaaggt cgcgcagcag ctgctggaca
    20341 gccatcgcgc ccagctcggc ggcaagccgc tggaattgct gctggacggg gcgccgaggc
    20401 tggtggtgga tgcgccggag tcggcggtgt cggtggcgct tggcaacctg atcggcaacg
    20461 cggtgaagta cacccagagc ggcgcggtga tcgtgcgcct gcacgacaac gcggtggacg
    20521 tgatcgattc cggtcccggt ctcagcgcgg aggatgccgc caggctgttc gagcgcggct
    20581 accgcggcac ccatgccggg cattcgcagg gcggcggcat cggcctgtcg atcgtgcgtc
    20641 gcctgtgcgc gctgtacggc tgggacgtgc atgtgcggcc gggcgagcag cgcggggtgg
    20701 tcgccaccct gcggttcgcg cagccgccgg cctagcggtc cgggcgtggc gcgatcctgc
    20761 cggcggcttc ggcggcataa tgcgcatgca tcgtcgccaa ggattcctgc atgactgccc
    20821 ctgcttccgc accgctggcc taccgggtcg cgctttccga ccatgcccgc agcgacgcgc
    20881 agcgggcggc gatcctcgaa ggcgatctcg gcttcggcag gcatttcacc gaccacatgg
    20941 tcgccatcca gtgggacaag gcgcagggct ggcacgatgc gcaggtccac gcctacggcc
    21001 cgctggtgct ggatccggcc gcggcggtgc tgcattacgg gcaggagatc ttcgaaggca
    21061 tcaaggccta tcgccacgcc gacggctcgg tctggacgtt ccgacccgac gccaacggcc
    21121 agcgcctgca acgctccgcc gcgcgcctga ccctgccgca gttgccggtg gccgagttca
    21181 ccgaatcgct gcgccagctg atcgccgtcg accacgcgtg ggtgccgtcg gcgccggaga
    21241 gcagcctcta cttccgtccc ttcatgatcg cgaccgaggc cttcctcggc gtgcgcccgg
    21301 cgcaggcggc ggcctacttc gtgatcgcca gccccgccgg cgcgtacttc gccaggggcg
    21361 tggcgccggt gtcgatctgg ttgtccgagg actacgcgcg cgcggccaag ggcggcaccg
    21421 gcgcggccaa gtgcggcggc aactatgccg cctcgctgtt gccgcagcag gaggcgcatg
    21481 cgcaaggctg ctcgcaggtg ctgttcctcg atccggtcga aggcaggtac ctggaggaac
    21541 tcggcggcat gaacgtgttc ctggtccacc gggatggccg gatcgtcacg ccggagctgt
    21601 ccggcagcat cctcgaaggc atcacccgcg ccagcgtgct gcaactggcc cgcgatcgcg
    21661 gcctggtggt ggaggagcgc aaggtgtcca tcgacgaatg gaagcagggc gtggcggcgg
    21721 gcgacatcac cgaggtgttc gcgtgcggga ccgcggcggt gatcaccccg atcggggagc
    21781 tgaagggcag ggatttcgcg gtgggcgaca tccacgcgcc ggccggcgag gtcaccatgg
    21841 cgatccgcag ggagctgacc gacatccagt acggccgggt tccggaccgc cacggctgga
    21901 tggccaggct gcgctagcag cagcggcgcg ctcgcctaca ggcggatcca ccagcagccg
    21961 aggtgtcggc gcaggccgaa caccgtgcgc gcgggcgtgc tgccatcggc cagggtctgt
    22021 tcgacgcgca ggccgaccag gcgcggcttg cgcggcggcg tgcccaccac ttcgccgtcc
    22081 gcgttgaact ccaccgcgtc gaagccgtat tcgtcgccgc ctgcgtacac cggctgcagg
    22141 tccaccagcg ggcgccgggc gatcgcctcg aggcgttcca tcacgcggta gccgttggcg
    22201 gtcgacatcc cggcccagtc gtagacccct gcgacctggt tggcatcgcc ggcctgcagc
    22261 gcgctgccca gcgcatgcac caggtcctgc acgctgcgcg aacagccgcc gcgcgcaggc
    22321 cgcagggcgc cgatgccttc ggccgccggc aaggccgggc gttcgcgggc gccgatgtcc
    22381 tcgcacttgc ggtcgctgta gaccagcacg ccgtcggcgc cgacgcactg gcgcacctgc
    22441 gccactgccg cgtccggcgc caaggcgcat gcaagcagca gcaggaagga caaggcgagg
    22501 cgcggcatgc gcgcagccta cgcgggcggc gcggatcgag gaagtgcgga acggccgcga
    22561 tggcgcggca tccggcaacc gcgatcgcgg cggccggcga ggccgtgctc aatccagccg
    22621 ggcgagcacg ttcagggtcg gcttggccag gttgagggta tagaagtgca gcgatggcgc
    22681 accgccggca agcagccgcc ggcacagccc ggccacgcag tccgccgcga actcgcggat
    22741 gccgtcggca tcgtcgccat aggcgcgcat gcgctggccg atccagcgcg ggatctccgc
    22801 gccgcaggct tcggaaaaac gccggagctg gctgaaattg gagatcggca tgatccccgg
    22861 cacgatcggg atgtcgatcc cgagcgcgcg cgcgtcgtcg acgaagcgga aatacgcgtc
    22921 ggtgttgtag aagtactggg tgatcgcgcc gttggcgccg gcatcgacct tggtcttgaa
    22981 gttgcgcagg tcaacgtgcg catcctcggc ctgcggatgg gtttccggat aggcggcgac
    23041 ctcgacctgg aaatggtcgc cgtgctcggc gcggatcagc gcgaccagtt cgctggcata
    23101 gcgcaggtcg ccgctgcggg ccatgccgga cggggtatcg ccgcgcagcg cgaccacgcg
    23161 gtcgcacccc agcgcgcggt acagcttgag cagggagcgg atctcctcgc ggctgccgcc
    23221 cacgcaggtg atgtgcggca ccccggcgac gccgtgctcg ctgcgcagcc gctgcacggt
    23281 ttccggggta tggctgaggg tcgagccgcc ggcgccgaag gtgaccgaca tgaactgcgg
    23341 cgcatgcgcc ttcagccgct gcacgctgcg gtcgagctgc gcgcgttgtt cgtcggtctt
    23401 gggcgggtag aactcgaagg aaaccggggc gcgcagggcc atgcaggggc tccgtggtgc
    23461 gtcgttgcgg ggcgcgatgc gggcggcccg ggggccgccc gcgcagggcg gtcagtagcg
    23521 gtagtgttcc ggcttgaacg ggccgtccac gtgcaccccg aggtagtcgg cctgctgctt
    23581 ggtcagcgtg gtcagcttca ccccgatctt ctccaggtgc aggcgcgcca cttcctcgtc
    23641 cagcttcttc ggcaggatgt agaccctggg gtcgtagaag tccttgttgg cccacaggtc
    23701 gatctgcgcc agggtctggt tggagaacga gttcgacatc acgaagctgg gatggccggt
    23761 ggcgcagccc aggttcacca gccggccttc ggccagcagg aagatcgcat tgccattggc
    23821 gaaggtgaac ttgtccacct gcggcttgat gttggtcttc accgcgccgc tggcatacag
    23881 cgcatcgacc tggatctcgt tgtcgaaatg gccgatgttg cagacgatgg cctggtcctt
    23941 catcgcctgc atgtgggcca gggtgatgat gtccttgttg ccggtggtgg tgacgtagat
    24001 gtcggcgcgg ccgagggtgt cctcgagggt cgtcacttcg tagccttcca tcgccgcctg
    24061 cagcgcgcag atcgggtcga tctcggtgac gatcacgcgg gcgccgtagg cgcgcagcga
    24121 cgccgccgaa cccttgccga cgtcgccgta gc
//
LOCUS       DAORUK010000013        24011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_1682_length_24011_cov_18.337035, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000013 DAORUK010000000
VERSION     DAORUK010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 24011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24011
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1682_length_24011_cov_18.337035"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /gene="tssK"
                     /locus_tag="PK818_01760"
     CDS             <1..926
                     /gene="tssK"
                     /locus_tag="PK818_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HQY81492.1"
                     /translation="DENAEVIVRSSDSYKFAELLPAGPGKFKLSPDYIPPATAIGAAH
                     NLARWTRALRDLLVSRGQDFASVKRQRGIRAASTSAQEVMRVVMMQTFARHITLFQEH
                     ARLNAVSAYAMYQDLRRLVAEFSVFSEDIGYFGELAGKPRETELPDYDHEDLRRCFRI
                     GFDRAEALIKALTVGAEVGITLAFDGRFYRADLPANLFENDKTRFYLAFESEQKGADL
                     FARLSRTGKIASMDEMPRLLSAALFGLKIDLLPVPPEELPQKSPNTTYFLVDTTHPFW
                     QMIKNGRNIAVFSDLPSDSTVIKLFPVATQD"
     gene            943..2274
                     /locus_tag="PK818_01765"
     CDS             943..2274
                     /locus_tag="PK818_01765"
                     /inference="COORDINATES: protein motif:HMM:NF021377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HQY81493.1"
                     /translation="MSRLLHHFLPVFSFGLALDEKVTANQASEAAAAVTARARELIDR
                     AKSAALADGKRPEQADGAAFAVVAWIDEIMARNPAYLTGSIPLQVTMFNTNNAGNEFF
                     QHLSALKQDQDEVREVYYHAMLCGFVGQYYYENGDTGELGKLKELHGRQLPVAPAPIH
                     TLREEKITPQPYGVADPGGPKYPRQWDRTLLRIGALVALLIPLAYLVYILLAAPKPTI
                     TVPVQQALAAFPCSDLAVSADEDKGTVKVDGYVSRADDIAAVKQRVDGVEGVKSSAVN
                     VQLRIWPHCEVVKILAQYQARNSDSGYGLSITPSTGHSDRFIENENVIVKLQQANYDG
                     YLYVDYYTVNGAVAHMYPNTGEPDSGRLIQSAEQFEVGATPSKTWTVSAPFGQELITV
                     IASPTPLYAEALPEIQTAEEYLPKLRQMLDANRGNARLAATYLFMQTEPAR"
     gene            2271..5867
                     /locus_tag="PK818_01770"
     CDS             2271..5867
                     /locus_tag="PK818_01770"
                     /inference="COORDINATES: protein motif:HMM:NF025689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein"
                     /protein_id="HQY81494.1"
                     /translation="MNPLDFVERLFSSIGSVFSRIGYLYQQGSDTARKPWLRRIVVTL
                     VLLVVLAGLVFLGLALVPLVPMRFWQVLAICLVALVVLWWFTSGQRKASLKGRTRKRI
                     GDLGPGNAEDEREPLQRMGAAIAEAKRTIARSPDIDKGRAPLYRIPWLLFVGDQAADV
                     DGLLRAGCEVSPFPPPDKEEMDPDDVWRWWFFKSMIAIEMHPRVVADTNARLDRGLWY
                     QSLMKLADEREKLPLNGIVVAVAAQSLLGPADRLKDTATRLRRLVDEAMEHLQVQMPV
                     YFVVSGLDRLPGYAQVRATLPAEAFAQALGHRFADSEVVSAASSGRVDEILKPIEDRL
                     HALRMTALRAQATATQRRAVFDFVESLRQAQQGLSLFVTLMLEDNPFQRTPRWRGLYF
                     AGSADAAHRGGAFVADLFTRFLPSDQPLAGTSFRGNAGRMAVAGVGVLAMLGLSASIA
                     YGLSSARRDDTALLAQTRSACAEVADRTSSGRIEWVAGCGRTIESLEAAAGDTLLSFG
                     IRRADSDIDRLKQRVIEDFNNLILAPEDQMLATDINQGRAGIEHVLAITQRLRLLEEC
                     RSNDDGCRQRELPNNVSFDARSRLFAPFRSHDNNTRRDRDNASALFATYLGYLRWQKK
                     NVLDAEHDRLQGLLSKLLAGYTPRAADLRKWADGREAGIDLAHFWLPEGAVVGTEAGQ
                     AVTVSQAYTRDAWEGIVQPMLATLHEQAPDRQARIDDLRSTYFRDYFASWAKFQARFG
                     NGIGLWRGHYGDLLARAASHQNPYDFFFRAAQRHLYELPLDWPLSSRWAATWLQMKSR
                     WLSSWRPFGRFVADSFRFGGESIAPPVWLLAMHDTQARVLAGEQATFARAYLRLQAEG
                     SGEDVYQIASDLFASKGKADKPPASDYTALIEAVDKPGEAFATAFKGDDFGAWSVVQG
                     PSKLLLFLTVHRAGEFVQERWRESVVKPLAALPEDQQIEALYGQQGKLGAFVNDWLKP
                     FITEKERLPVKVGGVSMPLTPGYQAMVASERKFLPVLDDGPPFLAGSFTLAAPSELGA
                     LDEGEAGTVFEVECRERSYRASSAGESLADAKAQVFWSPSSCLQASIRVSFALPPDEV
                     AEPTQEFDPATSEMQAVEAPVEEVSLTRIYAGPEGFARLIEEFSGGVHAFTAEDFRES
                     YSPAQWGELRQKLAAARFRSARVFLQVELSDEMKQYLGASTARAEVPNVILE"
     gene            5916..7001
                     /gene="tssA"
                     /locus_tag="PK818_01775"
     CDS             5916..7001
                     /gene="tssA"
                     /locus_tag="PK818_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HQY81495.1"
                     /translation="MAIDVNELLAPIPGGDPAGSDASFSDQFDRIREARRADDAGLAQ
                     GEWQTELKVADWREAQSLAEDILLRTSKDLQAAVWLAEATIARDGLAGARDGFELLNG
                     LLDQYWDGMYPRADDGDLEERASKLAWFATYGGRALLAMQLNDDPQAPLNLLGWQDSR
                     EVDNLGRQNADAYQAALDEGRITGEAYDSRMLATAEPVIRERIELAQAAREAFARLST
                     RSDERLGREAPSLAAIDDALKKIQQVYVKVAAAKGIGGIAAPAEDAAADAGGSQPAVA
                     AGAVGGGVPLALDAGSLASKDAALRALGEIAGFFRRTEPHSPVAYLLDRAVYWANMPL
                     EQFLAEIIRDEGTLSSIRERVGLPPSY"
     gene            7208..7720
                     /gene="tssB"
                     /locus_tag="PK818_01780"
     CDS             7208..7720
                     /gene="tssB"
                     /locus_tag="PK818_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HQY81496.1"
                     /translation="MAKKESTQHKLDRVRAPRVQLTYDVEVGDAIEKKELPFVAGVVG
                     NFSGQPAEPLGKLKDRKFVNVDKDNYDDVLKGMKPRVQMQVENKLAGDDSKLGVELTF
                     RSLEDFSPERVVNQIDPLRKLLEARQKLADLRNKAAGNDKFEDLLNEVLQNTDKIAQL
                     SREVGPKSEE"
     gene            7782..9266
                     /gene="tssC"
                     /locus_tag="PK818_01785"
     CDS             7782..9266
                     /gene="tssC"
                     /locus_tag="PK818_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HQY81497.1"
                     /translation="MANTETQAAGAAAAEAVEGGLLDQILTQSKIARSDTERTRARDL
                     IAELVAQVSDGALTVSNDAITAIDARIAEIDRVLSEQLSAVMHAPEFQQLEGSWRGLK
                     YLVDNSETSTNLKIKVLNTTKKELLKDFKSASDFDQSALFKKLYEEEYGTFGGAPFAT
                     LIGDFEFGRHPEDMYLVEEISHVCAAAHAPFLSAASPELLGFDDFTELSGPRDLAKIF
                     DTVEYAKWKSFRASEDSRYVGLAMPHVLGRLPYGPDTAPVEAFNFVEATTGTDHNKYL
                     WMNAAYALGTKVTDAFAKYGWCAAIRGVEGGGLVEGLPTHTFATDDGEVALKCPTEIA
                     ITDRREKELADLGLIPLVHCKGTDYAAFFSTQSAQKAKEYNTDAANANARLSSQLQYI
                     FAVSRIAHYLKSMMRDKIGSFASKSSVELFLNNWIAQYVLLDDNASQEAKAQYPLREA
                     RIEVAEVPGKPGAYKSVAFLRPHYQLDELSVSLRLVAELPKSAR"
     gene            9307..9795
                     /locus_tag="PK818_01790"
     CDS             9307..9795
                     /locus_tag="PK818_01790"
                     /inference="COORDINATES: protein motif:HMM:NF017454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HQY81498.1"
                     /translation="MAGVDAFLKLDGIKGESTDKSHKDEIEILSFSWGCHSSTSIGQG
                     GGGGVGKATFSDFTFQKKVDISSPKLALIAARGDHIRSAKISVRKAGGAAGQVDYQVY
                     DLEKIYVTGVQISGGDGGVFYETLTLSPTKFKWEYKQQGDDGSLTGNMAVTVDRAANT
                     AQ"
     gene            9842..10282
                     /gene="tssE"
                     /locus_tag="PK818_01795"
     CDS             9842..10282
                     /gene="tssE"
                     /locus_tag="PK818_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HQY81499.1"
                     /translation="MRGLIYRLVADALGEDSGRSLSISEMRAEVSRDLENLLNTRSEA
                     ARLIPDSFKECRASSLTYGILDFSAYSLLSPTDRDRIRRSLEQAISLHEGRLSRVRIA
                     LEPQRELDRALRFRIDAMLDLGDDNEKVQFDAVLQLNTQTYLVR"
     gene            complement(10279..11856)
                     /locus_tag="PK818_01800"
     CDS             complement(10279..11856)
                     /locus_tag="PK818_01800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY81500.1"
                     /translation="MNEPFAYDQVEYPSLIHPQMHPSRLAAIARLHGIRAAAPGSCRL
                     LEVGCGDGLQLITLAMAYPESRFVGVDLSAKAIARGERMRESLGLANLQLVAADLMEW
                     QPGPHPFDYVVAHGFYSWVPEPVRQRLLRLCDMHLGPRGIAYVSYNALPGSHLRRMLW
                     EILQFHAGTDRDPATSVPRARECLDLLLLGMPDNTRYRTTLAGEIADLKARLNPQVLF
                     HDDLAPINDPCTLDQFMRAAGSHGLAFVAEASYHEMSLYNAHPDARAMLEEVAQDDLV
                     TKEQYLDFFTGRRFRQTILCRSAANPCPRADPAAIPELDLVTELAPAAGQPGTDGGMH
                     FTRRSSGGLDTNDTVVQAMLIECGSRHPQALPVASLLEAARDSVASERTVAEDTQRAA
                     TFLLRAFQAGLVELHVDAPRFARHASERPAASRLARHQVEAGETQVISLRPSIVQLED
                     PVTCGLLLALDGTRDRDAILSFLHGHLARTGTSAPTGDGASLAEALEAGIDDALERVC
                     ALALVCEAPGDTAGPAG"
     gene            complement(11849..13498)
                     /locus_tag="PK818_01805"
     CDS             complement(11849..13498)
                     /locus_tag="PK818_01805"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HQY81501.1"
                     /translation="MAEPQQPTAGDPPPHPYHPPLLRRLALQAILPMALVALLLFVLL
                     GWEASRLLPAGSPALSATLLRHGLLLLAGLLVVAASGWLVAVRTLRPITELTEAIERI
                     GRGEQVRLQIDRGEILGRLQRGVNEASQALARSRNRMESELGRTAVELADKNARLEAA
                     SQSRARFLAAASHDLRQPLYALTLFSSALRAGETDPAKLTRVLHIEECVASLDHLFSE
                     LLDLSRLETGAVQAAIADVRLDEVFDEVSRNFRMLAESRGLRLIVRKTDAWVRADRTM
                     LTRILNNLVSNALRYTDHGGVLVGVRHQRSGQVRIDVWDTGHGIAAEHQPRVFEEFYQ
                     VSHEHAHPGERQRGLGLGLATVRKLAELLGCRISLASIPARGTLVSLVMAAGTPAAHS
                     HVPDFGDAPLDVSGLRVLVVDDEVSILEGLRALLAEWGCDIRAVEDEAAALEALAGWA
                     QPPDLVISDLRLREGRSGVEVLRAIARHYRQDPQQPGFARLLVTGETRSDRIAEIAAS
                     RIPVLFKPVSPQRLREAMLAAVFAVQATRDDSGQPGRQIDE"
     gene            complement(13626..13702)
                     /locus_tag="PK818_01810"
     tRNA            complement(13626..13702)
                     /locus_tag="PK818_01810"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13666..13668),aa:Pro,seq:cgg)
     gene            13836..15209
                     /gene="hisS"
                     /locus_tag="PK818_01815"
     CDS             13836..15209
                     /gene="hisS"
                     /locus_tag="PK818_01815"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HQY81502.1"
                     /translation="MIKPRTPPGVMELLPRDQVAFQRMLDTIRQTFERFGFLPVETPV
                     MELSEVLLTKSGGETERQVYFVQSTGALEKSGEGLPELALRFDLTVPLARYVAEHEHE
                     LAFPFRRYQMQRVYRGERAQRGRFREFYQCDIDVVGKDVLSTRFDAEIPAVIAAVFER
                     LAIGEFTIQLNHRKLLRGYFEGLGIEGEAQFAVLRELDKLDKRGEDAVRATLAGDGFG
                     LAGEAIDRLMAFARVRSHGHDDALAKLDALGSGTALFEEGRAELGAILRQLRALGVDE
                     ARYAINLSIARGLDYYTGVVYETTLDAYPQIGSICSGGRYDNLASHYSKSKLPGVGIS
                     IGLTRLFYQLREAGLVGTVDSSVDVLVALLDDAGLEAALVMSQQLRAAGLNVETQLEP
                     RKLARQMQYADKAGIRFVAMRGEDEAARGVVAVKDLRRGQQFEVAGHELAAALLQARE
                     LREGALA"
     gene            15206..17104
                     /locus_tag="PK818_01820"
     CDS             15206..17104
                     /locus_tag="PK818_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HQY81503.1"
                     /translation="MSTMITLDGNSLTRAQLVEVANGAAVDLDPAALRKVERAAQFLA
                     AQVAREEPIYGVSTGFGSNADKLLGAHPLRDELPGAVRSGRSLHVELQDNLIVTHAVC
                     VGEPFAAEVVRAMLCIRINTLLRGHSGIRVQTLQALAAMLNAGIVPVVPQLGSVGASG
                     DLAPLSHLAIVLLGGGEAFVDGERMPGAEALRRKRLQPVSLSYKEGLALNNGTAQMLA
                     TGVLALHRLDGLLDTADLAAAMTIDAFAGRLGAFAPDVHALRPHPGQVRTAANLRALL
                     AGSTLADIPYHLVPRFRQWLPASWETPESQALGFDIGWDWVPFGQRHGREKFYARFRP
                     FRGGKKHQPQDSYSLRCIPQVHGAVRDALEQAKRVFDVELNAVTDNPLVFPDKQAEFV
                     EEQVISAGHFHGMPLALAMSYVKAAIPVLASISERRINKLVDPATNDGLPAFLIGNED
                     GTESGHMIVQYTAAAIVNDLASRAMPASVYSVPTSANAEDHVSMGANEARHVLAMADD
                     LGKVLGLELYTAAQALDLRRDMINAARDLADRSDAEAFASKVQGGPLADTDDRTGFVA
                     EVDGLRAELAAADAFRPGHAVAAAHAAIRERIAFLDRDRAMDGEVAAAVRMVADGSVL
                     AAARAAIR"
     gene            complement(17153..18952)
                     /locus_tag="PK818_01825"
     CDS             complement(17153..18952)
                     /locus_tag="PK818_01825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQY81504.1"
                     /translation="MLFGLALLLAGLGSARAMDPYPLAPGQSAIPLGAHLQFRADAQA
                     ADGATEAFARARAGEFAPVPDGNPTFGFQDGAYWFYLPVLNQHPDELHWLLVQEYALS
                     DQVDLYVRYPDGRVEHQASGDHQPFTNRFIRYRHPNFRIDLPLGQTVEVLVRVQSESS
                     MQVPLVLYTPRALTEIMRDTQFSTGLYYGIVLALLFYNLVLWIMLRDPGYGWYLLHTG
                     AFGLVLFTLNGYGFEYFWPNWPWLADAAVPLSICLALIGMQQFSRSFLELRDRWPAGN
                     LASLALIGLFALLGIASTWLPYSTATPLASRMVLLGVIWIIIAAVVMLRRGYKPARLF
                     LLAWSFFLLGTAAFTLLAFGMLPSNFWTQNGVQIGSALEMLLLSLALGSRYAGLRNEN
                     IRIVQQTNEQLERSVIDRTKQLRTAMAQLGEANVQLREFSRRDPLTGVYNRRHFRETL
                     EQALDARSGDGRPLALLLLDLDHFKRINDRYGHLAGDDCLNFAAHGMEEVVVPRGGMV
                     ARFGGEEFVAVLPGADAQEALRIAEAVRARIEQSPVASCGHSIRLSASIGVHSVAGGQ
                     QATPEDVIRIADEALYRAKDDGRNCVRHSVSVA"
     gene            19083..20336
                     /locus_tag="PK818_01830"
     CDS             19083..20336
                     /locus_tag="PK818_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin-like phosphoesterase C-terminal
                     domain-containing protein"
                     /protein_id="HQY81505.1"
                     /translation="MRRIIALLLLGLWGWCGPAPAAAPGMRILLFGDPQVKSEQDLDY
                     FRRDIVEPLRGRHRAQLGISLGDIVDDVPALLPAVRRETERLGVPWLHVPGNHDVDPD
                     AQDDAGSLRSFRRGIGPDTMVRETALANVVVLDDVIALPGRKPAYIGGFRDDQFAQLE
                     ARLPALRKDRLLVVALHIPLFEEPGKDSFRDGDRERLFALLRDFPHVLVVSAHSHAQR
                     HVFHGAASGWQGPAPLHEYNVGTASGAYWSGVKDAAGIPDATMADGTPNGYAVLDVMA
                     GGTYALAWHNARDAADTSIGLHAPRVLRRGAYPGWGVFANVYMGMDDTRVEYRIDDGG
                     WLPMQKVLQPDPALLAENMRDDAAEALRGFDRSPEAEVSQHLWRGALPTTLDAGEHRI
                     EVRAFDRWRGEVRAGTRYRLQDRKP"
     gene            complement(20446..21174)
                     /gene="mtnC"
                     /locus_tag="PK818_01835"
     CDS             complement(20446..21174)
                     /gene="mtnC"
                     /locus_tag="PK818_01835"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HQY81506.1"
                     /translation="MQTTILTDIEGTTSSISFVKDVLFPYARRELPRFVRERGDEPEV
                     RRWLDMVAAEHGGICQDAVIVETLQGMIDQDRKHTALKALQGMIWLEGYRSRDFSAHV
                     YPDVAPRLRAWRDAGHQLAVYSSGSVAAQKLLFGNSDAGDLLPLFDAFFDTEAGHKRD
                     ADSYRLIADRLHRAVGDVLFLSDVVAELDAAREAGMRTVLLDRREDYPQPRIGEDATH
                     GHLRVESFDGIQPQAASIASLWRG"
     gene            complement(21183..21743)
                     /locus_tag="PK818_01840"
     CDS             complement(21183..21743)
                     /locus_tag="PK818_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone dioxygenase"
                     /protein_id="HQY81507.1"
                     /translation="MSRLRIFDEHDPATPLLETRDHAAIAAELARIGVEFRQWQAVHP
                     VQPGDPAEAIIAAYQADIDRLVQERGFRSVDVVSIAPDHPQREALRAKFLDEHFHKED
                     EVRFFVAGNGLFSLHVGQQVHEVLCEAGDLISVPDAATHWFDMGPEPSFVAIRFFTEP
                     DGWVGHFTGTDIARRFPRHEAGQANA"
     gene            complement(21769..22404)
                     /locus_tag="PK818_01845"
     CDS             complement(21769..22404)
                     /locus_tag="PK818_01845"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HQY81508.1"
                     /translation="MDTPAFDHNLFAGCAARIVSHTRELAAKGWTPATAGNFSIRMGP
                     GHAAITISGKDKGRLTEADIMVVDMANQPVATHHRPSAETALHTHLYRRFPDVGSVLH
                     THSSNQTVAGLLHADAGRVRLRGYELFKALRGHDTHEAELDLAVVPNSQDMDALIASV
                     DALLDTPNTWGYLIAGHGLYAWGHDIAEARRHLDAFEFMLGCELDLRRLRA"
     gene            complement(22480..23139)
                     /locus_tag="PK818_01850"
     CDS             complement(22480..23139)
                     /locus_tag="PK818_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HQY81509.1"
                     /translation="MHDLERAARQSLIDTCIAMNASGLSLGKSGNASLRWGDGLLISP
                     TGTAYAQLRPEHIVYLDADGNCPAGQLLPSSEWRIHCDILRAFPACEAVVHVHSTHAT
                     ALACLGQEIPPFHYMVAMAGGARIPCADYATFGTPELSANVLRALDGGLRACLMANHG
                     QVAAGKSLADALALAEEVENLARQYILARSLGPTRDLPAEEMDEVLRRFRHYGQQRRT
                     E"
     gene            complement(23217..>24011)
                     /locus_tag="PK818_01855"
     CDS             complement(23217..>24011)
                     /locus_tag="PK818_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003111532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HQY81510.1"
                     /translation="PRVAAIVASHEHPEFIVNVKETGQIQLVNYEDIGNLKITTIGAA
                     RFLHDGGWDSTRRYFMTAANQSDKIAVVDSRTQKLAAMVDVDKIPHPGRGANFVHPTC
                     GPVWATSALGNEKITLIGTDPDKHPSHAWKVCEVLLGQGGGSLFIKTHPKSRNLWVDT
                     PLNPDPKASQSIAVFDIGNLKSGFKVLPIAEWANLGEGPKRVVQPEYNAAGDEVWFSV
                     WNGKTQRSALVVVDDKTRKLKAVLNDKRLVTPTGKFNVYNTVKDVY"
BASE COUNT         3860 a         8147 c         8379 g         3625 t
ORIGIN      
        1 tggacgagaa cgccgaggtg atcgtgcgct cctccgacag ctacaagttc gccgaactgc
       61 tgcccgccgg ccccggcaag ttcaagcttt cgcccgacta catcccgccg gccaccgcga
      121 tcggcgccgc gcacaacctg gcgcgctgga cccgcgcgct gcgcgacctg ctggtctcgc
      181 gcgggcagga cttcgcctcg gtgaagcgcc agcgcggcat ccgcgcggcc tccaccagtg
      241 cgcaggaggt gatgcgggtg gtgatgatgc agaccttcgc ccgccatatc accctgttcc
      301 aggagcacgc gcggctgaac gcggtgtcgg cgtacgcgat gtaccaggac ctgcgccggc
      361 tggtggcgga gttctcggtg ttctcggagg acatcggcta cttcggcgag cttgccggca
      421 agccgcgcga gaccgagctg ccggactacg accacgagga cctccggcgc tgcttccgga
      481 tcggcttcga ccgcgcggag gcgctgatca aggcgctgac cgtcggtgcg gaggtcggca
      541 tcaccctcgc gttcgacggt cgtttctacc gcgccgacct gcccgccaac ctgttcgaga
      601 acgacaagac ccgcttctac ctggcgttcg agtcggagca gaagggcgcc gacctgttcg
      661 cgcggctgtc gcgcaccggc aagatcgcct cgatggacga gatgccgcgg ctgctgtccg
      721 cggcgctgtt cgggctgaag atcgacctgc tgccggtgcc gccggaagag ctgccgcaga
      781 agagcccgaa caccacctat ttcctggtcg ataccaccca tcccttctgg cagatgatca
      841 agaacggccg caacatcgcg gtgttctcgg acctgccgtc cgactcgacc gtgatcaagc
      901 tgttcccggt cgccacccaa gactgaaggc aggactgaac gcatgtcccg actcctgcat
      961 cacttcctcc cggtgttctc gttcggcctg gcgctggacg agaaagtcac cgccaaccag
     1021 gccagcgagg ccgcggcggc ggtgaccgcg cgggcgcgcg agctgatcga ccgcgccaag
     1081 tcggcggcgc tggccgatgg caagcgcccg gagcaggccg atggcgcggc gttcgcggtg
     1141 gtggcctgga tcgacgagat catggcgcgc aaccccgcct acctgaccgg cagcatcccg
     1201 ctgcaggtca ccatgttcaa caccaacaac gccggcaacg aattcttcca gcacctgtcg
     1261 gcgctgaagc aggaccagga cgaagtgcgc gaggtgtact accacgcgat gctgtgcgga
     1321 ttcgtcggcc agtactacta cgagaacggc gacaccggcg agctcggcaa gctgaaggaa
     1381 ctgcacgggc gccagttgcc ggtcgcgccg gcgccgatcc acaccctgcg cgaggagaag
     1441 atcaccccgc agccctacgg cgtggccgat cccggcggcc ccaagtatcc ccgccagtgg
     1501 gaccgcaccc tgctgcgcat cggtgcgctg gtcgcgctgc tgatcccgct ggcctatctc
     1561 gtgtacatcc tgctggcggc gccgaagccg acgatcaccg tgccggtgca gcaggcgctg
     1621 gccgcgttcc cgtgctcgga cctcgcggtc agcgcggacg aggacaaggg cacggtcaag
     1681 gtcgatggct acgtctcccg cgccgacgac atcgccgcgg tcaagcagcg cgtggacggg
     1741 gtggaaggcg tgaagtcctc cgccgtgaac gtgcagctgc ggatctggcc gcactgcgaa
     1801 gtggtgaaga tcctggccca gtaccaggcg cgcaacagcg acagcggcta cggcctgtcg
     1861 atcaccccga gcaccgggca ttcggatcgc ttcatcgaga acgaaaacgt gatcgtcaag
     1921 ctgcagcagg cgaactacga cggctacctg tacgtggact actacaccgt caacggcgcg
     1981 gtcgcgcaca tgtacccgaa caccggcgag cccgacagcg gccgcctgat ccagtcggcc
     2041 gagcaattcg aggtcggcgc caccccgtcc aagacctgga ccgtttcggc gccgttcggg
     2101 caggagctga tcaccgtgat cgcctcgccg acgccgctgt acgcggaggc gctgccggag
     2161 atccagaccg ccgaggaata cctgccgaag ctgcggcaga tgctggacgc caaccgcggc
     2221 aacgccaggc tcgccgcgac ctacctgttc atgcagacgg agcccgcacg atgaatccgc
     2281 tggacttcgt cgaacgcctg ttctcgagca tcggctcggt gttttcgcgg atcggctacc
     2341 tgtaccagca gggctccgac accgccagga agccatggct gcgccggatc gtggtcaccc
     2401 tggtgctgct ggtggtactc gccgggctgg tgttcctcgg gctggccctg gtcccgctgg
     2461 tgccgatgcg cttctggcag gtgctggcga tctgcctggt cgcgctggtg gtgctgtggt
     2521 ggttcacctc gggccagcgc aaggcctcgc tgaaggggcg cacgcgcaag cgcatcggcg
     2581 acctcggccc gggcaatgcc gaggacgagc gcgagccgct gcagaggatg ggcgcggcga
     2641 tcgccgaggc caagcgcacc atcgcgcgct cgccggacat cgacaagggc cgcgcgccgc
     2701 tgtaccgcat tccgtggctg ctgttcgtcg gcgaccaggc cgccgatgtc gacgggctgc
     2761 tgcgcgcagg ttgcgaggtc tcgccgttcc cgccgccgga caaggaggag atggatcccg
     2821 acgatgtctg gcgctggtgg ttcttcaagt cgatgatcgc gatcgagatg cacccgcggg
     2881 tggtcgccga caccaatgcg cggctcgacc gcggcctgtg gtaccagtcg ctgatgaagc
     2941 tcgccgacga gcgcgagaag ctgccgctga acggcatcgt ggtcgcggtc gcggcgcagt
     3001 cgctgctggg gccggccgac cggctgaagg acaccgcgac ccggctgcgc cggctggtcg
     3061 acgaggcgat ggagcacctg caggtgcaga tgccggtgta tttcgtggtc agcggcctcg
     3121 accgcctgcc gggctatgcg caggtccgcg cgaccctgcc ggccgaggcg ttcgcgcagg
     3181 cgctgggcca ccggttcgcc gacagcgaag tggtgagcgc cgccagcagc ggccgggtcg
     3241 acgagatcct gaagccgatc gaggatcgcc tgcacgcgtt gcggatgacc gccctgcgcg
     3301 cgcaggcgac cgccacccag cgccgcgcgg tgttcgactt cgtcgaatcg ctgcggcagg
     3361 cgcagcaggg cttgtcgctg ttcgtcaccc tgatgctgga agacaacccg ttccagcgca
     3421 ccccgcgctg gcgtggcctg tacttcgccg gcagcgcgga tgccgcgcac cgcggtggtg
     3481 cgttcgtcgc cgacctgttc acccgtttcc tgcccagcga ccagccactg gccggcacca
     3541 gtttccgcgg caacgccggg cggatggcgg tggccggggt cggggtgctg gcgatgctgg
     3601 ggctgtcggc gtcgatcgcc tatgggctgt cctcggcgcg gcgcgacgac accgcgctgc
     3661 tggcgcagac ccgcagcgcc tgcgcggagg tcgcggaccg cacgtccagc gggcgcatcg
     3721 agtgggtggc cggttgcggg cgcaccatcg agtcgctgga ggccgcggcc ggcgacaccc
     3781 tgctcagctt cggcatccgc cgtgcagaca gcgacatcga ccgcctcaag cagcgggtga
     3841 tcgaggactt caacaacctg atcctcgcgc ccgaggacca gatgctggcg accgacatca
     3901 accagggccg cgccggcatc gagcacgtgc tggcgatcac ccagcgcctg cgcctgctcg
     3961 aggaatgccg cagcaacgac gacggctgcc gccagcgcga gctgccgaac aacgtcagct
     4021 tcgacgcgcg ttcgcgcctg ttcgcgccgt tccgcagcca cgacaacaac accaggcggg
     4081 accgcgacaa cgcatcggcc ctgttcgcga cctacctcgg ctacctgcgc tggcaaaaaa
     4141 aaaacgtcct tgacgccgag cacgaccgcc tccagggcct gctgagcaag ctgctcgcgg
     4201 gctatacccc gcgcgccgcc gacctgcgga aatgggccga cggacgcgag gccggcatcg
     4261 acctcgcgca cttctggctg ccggaaggcg cggtcgtcgg caccgaggcg gggcaggcgg
     4321 tgacggtgtc gcaggcctac acccgcgacg cctgggaagg catcgtgcag ccgatgctgg
     4381 cgacgctgca cgagcaggcc ccggacaggc aggcgcgtat cgacgacctg cgcagcacct
     4441 atttccgcga ctacttcgcc agctgggcga agttccaggc ccgcttcggc aacggcatcg
     4501 gcctgtggcg cggccactac ggcgacctgc tggcgcgtgc cgccagccac cagaatccct
     4561 acgacttttt ttttcgcgcc gcccagcgcc acctgtatga gctgccgctg gattggccgc
     4621 tctcgtcgcg ctgggcagcg acctggttgc agatgaagtc gaggtggctg tcctcgtggc
     4681 ggccgttcgg tcggttcgtg gcggactcgt tccggttcgg cggcgaaagc atcgcgccac
     4741 cggtctggct gctggcgatg cacgacaccc aggcgcgggt gctggccggc gagcaggcca
     4801 ccttcgcccg cgcctacctg cggctgcagg cggagggctc cggcgaggac gtctaccaga
     4861 tcgcctccga cctgttcgcc agcaagggca aggccgacaa gccgccggcc agcgactaca
     4921 ccgcgctgat cgaggcggtg gacaagccgg gcgaggcgtt cgccaccgcg ttcaagggcg
     4981 acgacttcgg cgcctggtcg gtggtgcagg gaccctcgaa gctgctgctg ttcctgaccg
     5041 tccaccgcgc cggcgaattc gtgcaggagc gttggcgcga aagcgtggtc aagccgctgg
     5101 cggcgctgcc ggaggaccag cagatcgagg cgctgtacgg ccagcagggc aagctcggcg
     5161 cgttcgtcaa cgactggctc aagcccttca tcaccgagaa ggaacggctg ccggtgaagg
     5221 tcggcggggt ttcgatgccg ctgaccccgg ggtaccaggc gatggtcgca tccgagcgca
     5281 agttcctgcc ggtgctcgac gacggtccgc cgttcctcgc cggcagtttc accctggccg
     5341 cgccgagcga actcggggcg ctggacgagg gcgaggccgg caccgtgttc gaggtggagt
     5401 gccgcgaacg cagctatcgc gccagctccg ccggcgagtc actggcggat gccaaggcgc
     5461 aggtgttctg gtcgccatcc agctgcctgc aggcaagcat ccgcgtgagt ttcgcgttgc
     5521 cgccggacga ggttgccgaa ccgacccagg aattcgatcc cgcgaccagc gagatgcagg
     5581 cggtcgaggc cccggtcgag gaggtcagcc tgacccggat ctatgccggc ccggaaggct
     5641 tcgcgcgcct gatcgaggag ttctccggcg gcgtgcacgc gttcaccgcg gaggacttcc
     5701 gcgaaagcta cagcccggcg cagtggggcg aactgcggca gaagctggcg gccgcgaggt
     5761 tccgcagcgc acgggtgttc ctgcaggtcg aactgtccga cgaaatgaag cagtacctgg
     5821 gcgcaagcac cgcgcgcgcc gaggtgccga acgtgatcct cgaatgacga tcttccaccg
     5881 ttaaccacag cctacgaatc cagcggagcc gcgccatggc catcgacgtc aacgaactcc
     5941 tcgccccgat ccccggcggc gacccggccg gcagcgatgc cagcttctcc gaccagttcg
     6001 accgcatccg cgaggcgcgc cgcgcggacg atgccggcct cgcccagggc gagtggcaga
     6061 ccgagctgaa ggtcgccgac tggcgcgagg cgcaaagcct ggccgaggac atcctgttgc
     6121 gcaccagcaa ggacctgcag gcggcggtat ggctggccga ggcgaccatc gcgcgggacg
     6181 ggctggccgg tgcgcgcgat ggcttcgagc tgctgaacgg gctgctcgac cagtattggg
     6241 acggcatgta cccgcgcgcc gacgatggcg acctggagga acgcgcgagc aagctggcgt
     6301 ggttcgccac ctacggcggc cgcgccctgc tggcgatgca gctgaacgat gacccgcagg
     6361 ccccgttgaa cctgctcggc tggcaggact cgcgcgaggt cgacaacctc ggccggcaga
     6421 acgccgacgc gtaccaggcg gcgctggacg agggccggat caccggggaa gcctacgaca
     6481 gccgcatgct ggccaccgcg gaaccggtga tccgcgagcg gatcgagctc gcgcaggcgg
     6541 cgcgcgaggc cttcgcgcgg ctgtcgaccc ggtccgacga acgcctcgga cgtgaggcgc
     6601 ccagcctggc cgcgatcgac gatgcactga agaagatcca gcaggtctac gtgaaggtgg
     6661 ccgcggccaa gggcatcggc gggatcgcgg cgccggccga agacgcggcc gccgacgccg
     6721 gcggcagcca gcctgcggtg gccgcgggcg cggtgggcgg gggcgtcccg cttgcgctgg
     6781 acgcgggcag cctggcgtcc aaggatgccg cgctgcgcgc gttgggcgag atcgccggct
     6841 tcttccgccg caccgagccg cacagcccgg tcgcgtacct gctggaccgc gccgtgtact
     6901 gggccaacat gccgctggag cagttcctgg cggagatcat ccgcgacgag ggcaccctgt
     6961 cctcgatccg cgagcgggtc ggcctgccgc cgtcctactg atgcccggcg cacgcaatcg
     7021 ccaagggtcg gcggccacgt cgaacggcat atgcccaagt ctggccgtcc gacggatgtt
     7081 gcgcccacgg ctgcgttcct agactcgttc caccatcaag cggagtccgc gcccggcgcg
     7141 acgctccgtc caactccaca ggcaccgcca cgcggtaccg cccaacaact cgcgcacggg
     7201 gatcgtcatg gccaagaagg aaagcaccca gcacaagctc gaccgcgtac gcgccccgcg
     7261 cgtgcagctg acctacgacg tcgaggtcgg cgatgccatc gagaagaagg agctgccgtt
     7321 cgtcgccggc gtggtcggca acttcagcgg ccagcccgcg gaaccgctgg gcaagctcaa
     7381 ggaccgcaag ttcgtcaacg tcgacaagga caactacgac gacgtgctca agggcatgaa
     7441 gccgcgggtg cagatgcagg tcgagaacaa gctggccggc gacgattcca agctcggggt
     7501 cgaactgacc ttccgcagcc tggaggattt ctcgccggag cgcgtggtca accagatcga
     7561 cccgttgcgc aagctgctgg aggcgcggca gaagctggcc gacctgcgca acaaggccgc
     7621 cggcaacgac aagttcgagg acctgctgaa cgaagtcctg cagaacaccg acaagatcgc
     7681 ccagctctcc cgcgaagttg gcccgaagtc cgaggaatga gcggcagccg ccgcccgatc
     7741 gcccgtctcc cgacgcactg aaccaagcga gcacacacac catggccaat accgaaaccc
     7801 aggcggcggg cgccgccgct gccgaagccg tcgaaggcgg gctgcttgac cagatcctca
     7861 cccagagcaa gatcgcccgt tccgacaccg agcgcacccg cgcccgcgac ctgatcgcgg
     7921 agctggtggc gcaggtcagc gacggcgcgc tcaccgtgtc gaacgatgcg atcaccgcga
     7981 tcgatgcgcg catcgccgag atcgaccggg tgctgtccga gcagctgtcg gcggtgatgc
     8041 atgcgccgga attccagcag ctggagggca gctggcgcgg gctgaagtac ctggtcgaca
     8101 actccgaaac cagcacaaac ctcaagatca aggtgctcaa cacgaccaag aaggaactgc
     8161 tcaaggactt caagagcgcg tccgacttcg accagagcgc gctgttcaag aagctctacg
     8221 aagaggaata cggcaccttc ggcggcgccc cgttcgcaac cctgatcggt gacttcgaat
     8281 tcggccgcca ccccgaggac atgtacctgg tggaggagat ttcgcacgtc tgcgccgcgg
     8341 cgcacgcgcc gttcctgagc gcggcctcgc cggagctgct gggcttcgac gacttcaccg
     8401 aactctccgg cccgcgcgac ctcgccaaga tcttcgacac cgtggaatac gcgaagtgga
     8461 agagcttccg cgcctccgag gattcgcgct acgtcgggct ggccatgccg cacgtgctcg
     8521 gtcgcctgcc gtacggcccg gacaccgcgc cggtcgaggc gttcaacttc gtcgaggcca
     8581 ccaccggcac cgaccacaac aagtacctgt ggatgaatgc cgcctacgcg ctgggcacca
     8641 aggtcaccga tgcgttcgcc aagtacggct ggtgcgcggc gatccgcggc gtcgagggcg
     8701 gtggcctggt cgaaggcctg cctacccaca ccttcgccac cgatgacggc gaagtggcgc
     8761 tgaagtgccc gaccgagatc gcgatcaccg accgccgcga gaaggaactc gccgacctcg
     8821 gcctgatccc gctggtgcat tgcaagggca ccgactacgc cgcgttcttc tccacccagt
     8881 cggcgcagaa ggccaaggaa tacaacaccg acgcggcgaa cgccaacgcg cgcctgtcct
     8941 cgcagctgca gtacatcttc gcggtcagcc gcatcgcgca ttacctgaag tcgatgatgc
     9001 gcgacaagat cggcagcttc gcgtcgaagt ccagcgtcga gctgttcctc aacaactgga
     9061 tcgcgcagta cgtgctgctc gacgacaacg cctcgcagga ggccaaggcg cagtacccgc
     9121 tgcgcgaggc gcggatcgag gtggccgaag tccccggcaa gcccggcgcc tacaagtccg
     9181 tcgcattcct gcggccgcac taccagctgg acgaattgag cgtgtcgctg cgcttggtcg
     9241 ccgagttgcc gaagtcggcg cgctgacatc ccgcattctt tctttcagta accaggagga
     9301 tccaccatgg ctggcgtaga tgcattcttg aagctcgacg gaatcaaggg cgagtcgacc
     9361 gacaagtccc acaaggacga aatcgagatc ctcagcttct cgtggggatg ccacagcagc
     9421 accagcatcg gccagggcgg cggtggcggc gtcggcaagg cgaccttcag cgatttcacc
     9481 ttccagaaga aggtcgacat ctcgtcgccg aagctcgccc tgatcgctgc gcgtggcgac
     9541 cacatcaggt ccgcgaagat ctcggtccgc aaggcgggcg gcgccgccgg ccaggtcgac
     9601 taccaggtct acgacctgga gaagatctac gtgaccggcg tgcagatcag tggtggcgat
     9661 ggcggcgtgt tctacgagac cctcaccctg tcgcccacca agttcaagtg ggagtacaag
     9721 cagcaaggcg acgatggcag cctgaccggc aacatggcgg tcaccgtgga tcgcgccgcg
     9781 aataccgcgc agtgacccgc gtgcgccacc ggacgccggc ggcgcacgac ccggttgaac
     9841 gatgcgtggc ctgatctacc ggctggtggc ggacgccctg ggtgaagact cggggcgttc
     9901 gctttcgatc agcgaaatgc gcgcggaagt ctcgcgcgac ctcgaaaacc tgctcaacac
     9961 caggtccgaa gcggcgcggc tgatcccgga ctccttcaag gagtgccgcg cgtcgtcgct
    10021 gacctacggc atcctcgact tctccgcgta cagcttgctc agcccgaccg accgcgaccg
    10081 catccggcgc tcgctggaac aggcgatcag cctgcatgag gggcgcctga gccgggtcag
    10141 gatcgcgctg gagccgcagc gcgagctgga tcgcgcgctc cgcttccgga tcgacgcgat
    10201 gctcgacctc ggcgacgaca acgagaaggt gcagttcgac gccgtcctcc agctcaacac
    10261 ccagacctac ctggtgcgct agccggccgg acctgcggtg tcgcccggcg cttcgcacac
    10321 cagcgcaagc gcgcagacgc gctccagcgc atcatcgatt cccgcctcga gggcctccgc
    10381 aagcgacgct ccgtcgcccg tgggggcgct ggtgcccgtg cgcgccagat ggccatgcag
    10441 gaaggagagg atcgcgtcgc gatcgcgggt tccgtccagc gcgagcagca atccgcaggt
    10501 caccggatcc tcgagttgca cgatcgaggg gcgcaggctg atcacctggg tctcgcctgc
    10561 ctcgacctgg tgcctggcca ggcggctggc cgccgggcgt tcggatgcat gccgggcgaa
    10621 gcgtggcgcg tcgacgtgca attccaccaa tccggcctgg aaggcacgca gcaggaacgt
    10681 ggctgcgcgc tgggtatcct cggcaacggt gcgttcgctg gcgacgctgt cgcgcgccgc
    10741 ttcgagcagg gacgccaccg gcagggcctg cggatgccgg ctgccgcact cgatcaacat
    10801 cgcctggacg accgtgtcgt tggtgtccag tccaccgctg gagcgtcggg tgaagtgcat
    10861 gccgccatcg gtgcccggtt gcccggccgc gggcgccagc tcggtcacca gatccagctc
    10921 cgggatcgcc gcgggatcgg cgcgcgggca cggattggcg gcggatcggc acaggatggt
    10981 ctgcctgaag cggcggccgg tgaagaagtc caggtattgc tccttggtca cgaggtcgtc
    11041 ttgcgccacc tcttccagca tggcccgtgc atccgggtgc gcgttgtaca gggacatctc
    11101 gtggtaggac gcctcggcca cgaaggcaag tccgtggctg ccggctgcgc gcatgaactg
    11161 gtcgagcgtg cacgggtcgt tgatcggggc gaggtcgtcg tggaacagca cctgcgggtt
    11221 caggcgggcc ttgaggtccg cgatctctcc ggcgagggtc gtccggtagc gcgtgttgtc
    11281 cggcatcccg agcagcagca ggtcgaggca ttcgcgcgcg cgcggcacgc tggtggcggg
    11341 gtcgcggtcg gtgccggcgt ggaattgcag gatctcccac agcattcgcc tcaggtggct
    11401 gccgggcaat gcgttgtagc tgacataggc gatgccgcgc gggcccaggt gcatgtcgca
    11461 cagccgcagc aggcgctggc gcaccggttc gggtacccac gagtagaagc cgtgggcgac
    11521 cacgtaatcg aaggggtgtg gcccaggctg ccactccatc aggtcggcgg cgaccagttg
    11581 caggttcgca agtcccagcg attcgcgcat ccgctcgccg cgggcgatcg ccttcgccga
    11641 caggtcgacg ccgacgaagc ggctttcggg gtacgccatg gccagggtga tcagttgcag
    11701 gccatcgccg cagcccactt ccagcagccg gcagctgccg ggcgccgccg cgcggatgcc
    11761 gtgcaggcgt gcgatcgcgg ccagccgcga ggggtgcatc tgcggatgga tcagcgacgg
    11821 atactcgacc tggtcgtacg cgaacggctc attcatcgat ctgtcttcct ggttgcccgc
    11881 tgtcgtcacg ggtcgcctgc acggcgaata ccgcggccag catcgcttcg cgcaaccgtt
    11941 gcggcgatac cggcttgaac agcaccggga tgcggctggc ggcgatctcg gcgatgcggt
    12001 cgctgcgggt ctcgccggtg accagcaggc gcgcgaatcc tggctgctgc gggtcctgcc
    12061 ggtagtggcg tgcgatcgcc cgcagcactt ccaccccgct gcggccctcg cgcaggcgca
    12121 ggtcgctgat caccaggtcc ggtggctgcg cccagccagc cagcgcctcc agcgcggcgg
    12181 cttcgtcctc gaccgcgcgg atgtcgcagc cccattcggc gagcagggcg cgcaggcctt
    12241 cgaggatgct gacctcgtcg tccaccacca gcacccgcag gccgctcacg tccagcggcg
    12301 cgtcgccgaa atccggcacg tgcgaatgcg ccgccggggt gccggccgcc atcaccagcg
    12361 agacgagggt gccgcgcgcg gggatcgacg ccagcgagat gcggcagccc agcagttccg
    12421 cgagcttgcg cacggtggca aggccaagcc ctagcccgcg ctggcgttcg cccggatgcg
    12481 cgtgctcgtg cgagacctgg tagaactcct cgaacacgcg tggctggtgc tccgcggcga
    12541 tcccgtggcc ggtgtcccag acgtcgatgc gcacctgccc ggagcgctgg tggcggaccc
    12601 cgaccagcac gccgccgtgg tcggtgtagc gcagcgcgtt gctgaccagg ttgttgagga
    12661 tgcgggtcag catggtgcgg tcggcgcgta cccacgcatc ggtcttgcgc acgatcaggc
    12721 gcagcccgcg cgattcggcg agcatgcgga agttgcggct gacctcgtcg aacacctcgt
    12781 ccaggcgcac gtcggcgatc gccgcctgca cggcgccggt ctccagccgc gacaggtcca
    12841 gcagttccga gaacaggtgg tccagcgagg ccacgcattc ctcgatgtgc agtacccggg
    12901 tgagcttggc cgggtcggtt tcgccggcgc gcaacgctga ggagaacagc gtcagcgcgt
    12961 acagcggttg ccgcaggtcg tggctggcgg cggccaggaa gcgcgcccgc gactggctgg
    13021 cggcctcaag ccgggcgttc ttgtcggcca gctccaccgc ggttcgcccg agttcgcttt
    13081 ccatccggtt gcgcgaacgc gccagcgcct gcgaggcttc gttgacgccg cgctgcaggc
    13141 ggccgaggat ctcgccgcgg tcgatctgca ggcgcacctg ttcgccacgg ccgatgcgtt
    13201 cgatcgcctc ggtcagttcg gtgatcggtc gcagcgtgcg caccgccacc agccagccgc
    13261 ttgccgcgac caccagcagg ccggccagca acagcagccc gtgccgcagc agggtggcgg
    13321 agagggcggg gctgccggcc ggcagcagcc gcgatgcctc ccagccaagc agcacgaaca
    13381 gcaggagcgc caccagcgcc atcggcagga tcgcctgcag cgccagccgc cgcagcagcg
    13441 gcgggtggta ggggtgcggc ggcggatcgc ccgccgtggg ttgctgcggt tccgccacgc
    13501 ggcacctggc gcaagggacg taccgttgca aacgcaggat ggctccggat gtggacatgc
    13561 ccggcatccc gcaggcatga aaaaaccgcc acgcgggcgg ttttctcgtc acacaatgca
    13621 tcgattggtc ggggagacag gattcgaacc tgcgacctct acgtcccgaa cgtagcgctc
    13681 taccagactg agctacaccc cgacggtgta gccgcgcatt gtaggcaatc gaccctgccc
    13741 gaggcaagac cctgcgctgc atgaggtccc cggatgggcc ctggaatggc cgaaacgcta
    13801 cacttgcggg ctttcccgcg ccggaatcga ccaccttgat caagccacgc accccgcccg
    13861 gcgtcatgga gctgctgccc cgcgaccagg tcgcgttcca gcgcatgctg gacaccatcc
    13921 gccagacctt cgagcgcttc ggcttcctgc cggtggaaac cccggtgatg gagctgtccg
    13981 aggtcctgct gaccaagtcc ggcggcgaaa ccgaacggca ggtctatttc gtccagtcca
    14041 cgggtgcgct ggagaagtcg ggagagggcc tgccggagtt ggccttgcgc ttcgacctga
    14101 cggtgccgct ggcgcgttac gtggccgagc acgagcacga gctggcgttc ccgttccgcc
    14161 gctaccagat gcagcgcgtg taccgcggcg agcgcgccca gcgtggccgc ttccgcgagt
    14221 tctaccagtg cgacatcgac gtggtcggca aggacgtgct gtcgacgcga ttcgatgcgg
    14281 agatcccggc agtgatcgcg gcggtgttcg agcgcctggc gatcggcgag ttcaccatcc
    14341 agctcaacca ccgcaagctg ctgcgtggct atttcgaggg cttgggcatc gaaggcgagg
    14401 cccagttcgc ggtcctgcgc gaactcgaca agctggacaa gcgcggcgaa gacgcggtgc
    14461 gcgcaaccct ggccggggac ggcttcgggc tggcaggcga ggccatcgac cggctgatgg
    14521 cgttcgcgcg ggtgcgctcg cacgggcatg acgatgcgct ggccaagctc gatgcgcttg
    14581 gcagcggcac cgcgctgttc gaggaaggcc gcgccgaact gggcgcgatc ctgcggcaat
    14641 tgcgcgcgct cggggtggac gaggcgcgct acgcgatcaa cctctcgatc gcgcgcggcc
    14701 tcgactacta caccggcgtg gtctacgaga ccacgctgga cgcctacccg cagatcggct
    14761 cgatctgttc cggcgggcgc tacgacaacc tcgccagcca ttacagcaag tcgaagctgc
    14821 ccggcgtggg catctcgatc ggcctgacca ggctgttcta ccagctgcgc gaggcgggcc
    14881 tggtcgggac cgtggacagt tcggtcgacg tgctggttgc gctgctcgac gatgccgggc
    14941 tggaggctgc gctggtgatg tcgcagcagc tgcgcgccgc cggcctgaac gtggaaaccc
    15001 aactggagcc gcgcaagctg gcgcggcaga tgcagtacgc cgacaaggcc gggatccgct
    15061 tcgtggccat gcgtggcgag gacgaggccg cgcgcggcgt ggtggcggtc aaggacctgc
    15121 gccgaggcca gcagttcgag gtcgcggggc acgaactggc ggccgcgctg ctgcaggcgc
    15181 gcgaactgcg cgagggagca ctggcatgag cacgatgatc accctggacg gcaattcgct
    15241 gacccgggcg caactggtcg aggtcgcgaa cggtgccgcc gtcgacctcg atcccgccgc
    15301 gttgcgcaag gtcgagcgcg ccgcgcagtt cctggccgcg caggtcgcgc gcgaagaacc
    15361 catctacggc gtctccaccg gcttcggcag caacgcagac aagctgctgg gcgcgcatcc
    15421 gctgcgcgac gaattgccgg gcgcggtgcg ctccgggcgc tcgctgcacg tcgagctgca
    15481 ggacaacctg atcgtgaccc acgcggtctg cgtgggcgag ccgttcgcgg cggaggtggt
    15541 gcgggcgatg ctgtgcatcc gcatcaacac cctgctgcgc gggcattccg ggatccgcgt
    15601 gcagacgctc caagcgctgg cggcgatgct caatgccggc atcgtgccgg tggtgccgca
    15661 gctgggatcg gtgggggcgt ccggcgatct tgcgccgctg tcgcacctgg ccatcgtgtt
    15721 gctgggcggc ggcgaagcct tcgtcgacgg cgagcgcatg ccgggcgccg aagcgctccg
    15781 gcgcaagcgg ctgcagccgg tctcgctgtc gtacaaggaa gggctggcgc tcaacaacgg
    15841 caccgcgcag atgctggcca ccggcgtgct ggcgctgcac aggcttgacg gcctgctgga
    15901 caccgccgac ctcgccgcgg cgatgaccat cgacgcgttc gccggccgcc tcggcgcgtt
    15961 cgcgccggac gtgcacgcgc tgcgcccgca tccggggcag gtccgcaccg ccgccaacct
    16021 gcgcgcgctg ctggcgggtt ccaccctggc cgacatcccg taccacctgg tgccgcggtt
    16081 ccgccagtgg ttgcccgcga gttgggagac gccggaatcg caggcgctgg gcttcgacat
    16141 cggctgggac tgggtgccgt tcggccagcg ccacggccgc gagaagttct acgcgcgctt
    16201 ccggccgttc cgtggcggca agaagcacca gccacaggac agctactcgc tgcgctgcat
    16261 cccgcaggtg catggggcgg tgcgcgatgc gctggagcag gccaagcgcg tgttcgatgt
    16321 cgagctcaac gcggtcaccg acaacccgct ggtgttcccg gacaagcagg cggaattcgt
    16381 cgaggaacag gtgatctccg ccggccactt ccacggcatg ccgctggcat tggcgatgag
    16441 ctacgtgaag gcggcgatcc cggtgctggc ctcgatcagc gagcgccgga taaacaagct
    16501 ggtcgacccg gccaccaacg atggcctgcc ggcgttcctg atcggcaacg aggacggcac
    16561 cgagagcggg cacatgatcg tgcagtacac cgccgcggcg atcgtcaacg accttgccag
    16621 ccgggcgatg ccggcctcgg tgtattcggt cccgaccagc gccaacgccg aggaccatgt
    16681 gtcgatgggc gccaacgagg cgcgccacgt gctggcgatg gcggacgacc tgggcaaggt
    16741 gctgggcctg gagctgtaca ccgccgcgca ggcgctggac ctgcgccgcg acatgatcaa
    16801 tgccgcgcgt gacctcgcgg accgcagcga tgcggaagcg ttcgcgtcga aggtgcaggg
    16861 cggtccgctg gcggataccg acgatcgcac cggcttcgtc gccgaagtgg atggcctgcg
    16921 tgcggaactg gccgcggccg atgcgttccg ccccgggcat gcagtggcgg cggcgcatgc
    16981 cgcgatccgc gaacgcatcg cgttcctcga ccgcgaccgc gcgatggatg gggaagtggc
    17041 ggcggcggtg cggatggtcg cggatggcag cgtactggcc gcggcgcggg ctgcgatcag
    17101 gtagcaacgg cagcatcccg gggcggtggc gcggccccgg cgaggatcgg gctcaggcca
    17161 cgctgaccga gtgccgcacg cagttgcggc cgtcgtcctt ggcccggtac agggcctcgt
    17221 cggcgatccg gatcacgtct tccggggtcg cctgctggcc gccggcaacg ctgtgcacgc
    17281 cgatgctcgc actcaggcgg atgctatgtc cgcagctggc gaccggcgac tgttcgatgc
    17341 gcgcgcgcac cgcctcggcg atccgcaagg cctcctgtgc atccgcgcca ggcagcaccg
    17401 cgacgaattc ctcgccgccg aagcgcgcga ccatgccccc acgcggcacg accacttcct
    17461 ccatcccgtg ggcggcgaaa ttcaggcagt cgtcgcccgc aaggtggcca tagcggtcgt
    17521 tgatccgctt gaaatggtcg aggtccagca gcagcagtgc cagtggcctg ccatcgccgc
    17581 tgcgtgcatc cagtgcctgc tcgagggtct cgcggaaatg gcggcggttg tagacgccgg
    17641 tcagcgggtc gcgccggctg aattcacgca gctgcacgtt ggcttcgccc agctgcgcca
    17701 tcgccgtgcg caattgcttg gtccggtcga tcacgctgcg ttcaagctgc tcgttggtct
    17761 gctggacgat gcggatgttc tcgttgcgca ggccggcgta gcgcgagccc agcgccagcg
    17821 acagcagcag catctccagc gcggagccga tctgcacgcc gttctgggtc cagaaattgc
    17881 tgggcaacat gccgaaggcc agcagggtga acgccgcggt gcccaacagg aagaacgacc
    17941 acgccagcag gaacagccgg gcgggcttgt acccgcggcg cagcatcacc accgccgcga
    18001 tgatgatcca gatgacgccc agcagcacca tccgcgacgc cagcggcgtc gcggtgctgt
    18061 agggcagcca ggtcgaggcg atgccgagca gcgcgaagag gccgatcagg gccaggctgg
    18121 ccaggttgcc ggccggccag cgatccctca gttccaggaa gctgcgcgag aactgctgca
    18181 tgccgatcag cgccaggcag atcgacagcg gcaccgcggc gtccgccagc catggccagt
    18241 tcggccagaa atattcgaag ccgtatccgt tgagggtgaa cagcaccagc ccgaatgccc
    18301 cggtatgcag caggtaccag ccgtagccgg ggtcgcgcag catgatccac aacaccaggt
    18361 tgtagaacag cagcgccagc acgatcccgt agtacaggcc ggtggagaac tgggtgtcgc
    18421 gcatgatctc ggtcagcgcg cgcggcgtgt acagcaccag tggcacctgc atcgagcttt
    18481 cgctctgcac ccgcaccagc acctccaccg tctgccccag cggcaggtcg atccggaagt
    18541 tggggtgccg gtagcggatg aagcggttgg tgaacggctg gtggtcgccg ctggcctggt
    18601 gctcgaccct gccgtccggg tagcggacat agaggtcgac ctggtcgctg agcgcgtact
    18661 cctgcaccag cagccagtgc agttcgtcgg ggtgctggtt cagcaccggc aggtagaacc
    18721 agtaggcacc gtcctggaaa ccgaaagtcg ggttgccatc cggcaccggc gcgaactcgc
    18781 cggcccgcgc ccgcgcaaag gcctcggtgg cgccatccgc cgcctgtgcg tccgcgcgga
    18841 actggaggtg ggcgccgagc gggatcgcgg attggcccgg ggcaagcggg tacggatcca
    18901 tcgcgcgcgc gcttccaagc cccgccagca gcagggcaag tccgaacagc atcgaccgca
    18961 agccaatccc gatcccgagc aactgtcccg cctgccttcc ggcacccccg ccgacgattc
    19021 ccggattcta gtgcaaacag gtgtcgcaac ggcagcggca taatccccgg caaggagggg
    19081 acatgcgcag gatcatcgcg ttgttgctgc tgggcctttg gggctggtgc gggccggcgc
    19141 ccgcggccgc gcccgggatg cggatcctgc tgttcggcga cccgcaggtg aagtcggagc
    19201 aggacctcga ttatttccgc cgcgacatcg tcgagccgct gcgcggccgc catcgcgcgc
    19261 agctggggat cagcctcggc gacatcgtcg acgacgtgcc ggcgctgttg ccggcggtca
    19321 ggcgcgagac cgagcggctc ggggttccct ggctgcacgt gcccggcaac cacgacgtcg
    19381 atccggatgc gcaggatgat gccggttccc tgcgcagctt ccggcgcggg atcggccccg
    19441 acaccatggt ccgcgaaacc gcgctggcga acgtcgtggt gctggacgac gtgatcgcgc
    19501 tgcccggccg caagccggcc tacatcggcg gcttccgcga cgaccagttc gcacagcttg
    19561 aggcgcggct accggcgctg cgcaaggacc gcctgctggt ggtcgcgctg cacatcccgc
    19621 tgttcgagga gccgggcaag gacagcttcc gcgacggcga tcgcgagcgc ctgttcgcgc
    19681 tgttgcgcga tttcccgcac gtgctggtgg tgagcgcgca cagccacgcc cagcgccacg
    19741 tcttccacgg cgccgcgagc ggttggcagg ggccggcgcc actgcacgaa tacaacgtcg
    19801 gcaccgccag cggcgcgtac tggtcgggcg tgaaggatgc cgcgggcatc ccggacgcga
    19861 ccatggccga cggcaccccg aatggctacg cggtgctcga cgtgatggcg ggcggcacct
    19921 atgcgctggc ctggcacaac gcgcgcgacg ccgccgacac cagcatcggc ctgcatgcgc
    19981 ccagggtcct gcggcgcggc gcctacccgg ggtggggcgt gttcgccaac gtctacatgg
    20041 gcatggacga cacccgcgtc gaataccgca tcgacgacgg cgggtggctg ccgatgcaga
    20101 aggtcctgca gcccgatccc gcgctgctgg cggaaaacat gcgcgacgat gcggcggagg
    20161 cgttgcgcgg tttcgaccgt tcccctgaag cggaagtttc gcagcacctg tggcgcggcg
    20221 ccctgccgac cacgcttgat gccggcgaac accgcatcga agtgcgcgcg ttcgaccgct
    20281 ggcgcggtga agtgcgggcc gggacccgct atcgcctgca agaccgaaag ccatagtcgg
    20341 tcggggtgcg gcgctgtggc gctgtggcgg ggatagggtt cgagcgcgcc atggatggcg
    20401 cgcggccgcg catcggagcc ggatgcggag cctaagcggc gaaccctacc cccgccacag
    20461 cgacgcgatc gacgcagcct gaggctggat cccgtcgaac gattccaccc gcaggtggcc
    20521 atgggtggcg tcttcgccga tgcgcggctg cggatagtcc tcgcggcgat ccagcagcac
    20581 ggtgcgcatg ccggcctcgc gcgcggcatc gagttcggcg accacgtcgg acaggaacag
    20641 gacatcgccg accgcacggt gcagccggtc cgcgatcagc cggtagctgt ccgcatcgcg
    20701 cttgtggccg gcttcggtat cgaagaaggc atcgaacagc ggcagcaggt cgccggcatc
    20761 cgaattgccg aacagcagct tctgcgccgc caccgacccg gacgagtaca ccgcgagctg
    20821 gtggccggcg tcgcgccatg cgcgcagccg cggggcgacg tccggataca cgtgcgcgct
    20881 gaaatcgcgg ctgcgatagc cctccagcca gatcatgccc tgcagcgcct tcagcgcggt
    20941 gtgcttgcgg tcctggtcga tcatgccctg cagggtctcg acgatgaccg cgtcctggca
    21001 gatgccgcca tgctcggcgg ccaccatgtc cagccagcgg cggacctcgg gctcgtcacc
    21061 gcgctcgcgc acgaagcgcg gcagttcgcg gcgcgcgtac gggaacagca cgtccttgac
    21121 gaacgagatg ctggaggtgg tgccttcgat gtcggtgagg atcgtggtct gcatcggatc
    21181 gctcaggcgt tggcctggcc cgcctcatgg cgcgggaacc ggcgggcgat gtcggtaccg
    21241 gtgaaatggc cgacccagcc gtccggctcg gtgaagaagc ggatcgcgac gaagctaggc
    21301 tccgggccca tgtcgaacca gtgggtggcg gcatcgggca ccgagatcag gtcgccggcc
    21361 tcgcacagca cttcatgcac ctgctggccc acatgcagcg agaacaggcc gttgccggcc
    21421 acgaagaagc gcacttcgtc ttccttgtgg aagtgctcgt ccaggaactt ggcgcgcaat
    21481 gcttcgcgct gcgggtggtc cggcgcgatg ctcaccacgt ccacgctgcg gaagccgcgt
    21541 tcctgcacca ggcggtcgat gtcggcctgg taggcggcga tgatcgcctc cgccggatcc
    21601 cccggctgca ccggatgcac ggcctgccat tgccggaact cgaccccgat cctggccagt
    21661 tccgccgcga tcgcggcatg gtcgcgggtt tccagcagcg gcgtggcggg gtcgtgctcg
    21721 tcgaagatgc gcaggcggct caaggcggtg gtccgtgcag gggaggggtc aggcgcgcag
    21781 gcgtcgcagg tcgagctcgc agccgagcat gaactcgaac gcgtcgaggt ggcggcgggc
    21841 ttcggcgatg tcgtggcccc acgcatacag gccgtggccc gcgatcaggt aaccccaggt
    21901 gttgggggtg tccagcagcg cgtcgacgct ggcgatgagg gcgtccatgt cctgcgaatt
    21961 cggcaccacc gcaaggtcca gctcggcctc gtgggtgtca tgcccgcgca gcgccttgaa
    22021 cagctcgtag ccgcgcaggc gcacccggcc ggcgtcggcg tgcagcagcc cggcgacggt
    22081 ctggttgctc gaatgcgtgt gcagcaccga gccgacgtcc gggaagcgcc ggtagaggtg
    22141 ggtgtgcagg gcggtttccg cggagggacg gtggtgggtc gcgaccggct ggttcgccat
    22201 gtccaccacc atgatgtcgg cttcggtcag gcggcccttg tccttgccgg aaatggtgat
    22261 cgcggcgtgg ccgggcccca tccggatcga gaaattgccg gcggtggccg gggtccagcc
    22321 cttcgccgcc agctcgcggg tatgcgagac gatccgggcg gcgcagccgg caaacaggtt
    22381 gtggtcgaag gcgggggtgt ccattcggcg aagtgtagcg gctgcatgtg cccgcctaca
    22441 tgacgatcgc gcccgggttc atgcccggtt gcagccggat cattcggtgc ggcgctgttg
    22501 cccgtagtgg cggaagcggc gcaggacttc gtccatttcc tccgccggca ggtcgcgggt
    22561 gggtcccagg ctgcgcgcga ggatgtactg gcgggccagg ttctccactt cctccgccag
    22621 cgccagcgcg tcggccagcg acttgccggc cgccacctgg ccgtggttcg ccatcaggca
    22681 ggcgcggagc ccgccatcca atgcgcgcag gacgttggcg gacagctcgg gggtgccgaa
    22741 ggtcgcgtag tcggcgcagg ggatgcgggc accaccggcc atcgccacca tgtagtggaa
    22801 cggcgggatc tcctggccca ggcaggccag cgcggtggcg tgggtcgagt gcacgtggac
    22861 caccgcctcg caggccggga atgcacgcag gatgtcgcag tggatccgcc actcgctgga
    22921 cggcagcaat tgcccggccg ggcagttgcc gtcggcatcc aggtagacga tgtgctccgg
    22981 ccgcaactgc gcgtatgcgg tgccggtggg cgagatcagc aggccatcgc cccagcgcag
    23041 gctggcgttg cccgacttgc ccagcgacag gccgctggca ttcatcgcga tgcaggtgtc
    23101 gatcagggat tggcgcgctg cgcgttcgag gtcatgcatg cgggaattcc ggggggctgc
    23161 catgaaaaac cccgccagca tgcgctggcg gggtcgtggg gcaccgatgg ggcgcgtcag
    23221 tagacgtcct tcaccgtgtt gtagacgttg aacttgccgg tgggcgtgac caggcgcttg
    23281 tcattcagca ctgccttcag cttgcgggtc ttgtcgtcca ccaccaccag cgcggaacgc
    23341 tgggtcttgc cgttccacac cgagaaccag acctcgtcgc cggccgcgtt gtactccggc
    23401 tggaccaccc gcttcgggcc ttcgccgagg ttcgcccatt cggcgatcgg caggaccttg
    23461 aagccggact tgaggttgcc gatgtcgaac accgcgatcg actggctggc cttcggatcc
    23521 gggttaagcg gggtgtccac ccacaggttg cgggacttcg ggtgggtctt gatgaacagc
    23581 gaaccgccgc cctggccgag cagcacttcg cacaccttcc aggcatggga cgggtgcttg
    23641 tcggggtcgg tgccgatcag ggtgatcttc tcgttgccca gtgccgaggt cgcccacacc
    23701 ggcccgcagg tcgggtgcac gaagttggcg ccgcggcccg ggtgcgggat cttgtccacg
    23761 tcgaccatcg ccgccagctt ctgggtgcgc gaatccacca ccgcgatctt gtcgctctgg
    23821 ttggccgcgg tcatgaagta gcgacgggtg gaatcccagc cgccgtcgtg caggaagcgc
    23881 gcggcaccga tggtggtgat cttgaggttg ccgatgtcct cgtagttcac cagctggatc
    23941 tgcccggttt ccttgacgtt gacgatgaac tcggggtgct cgtgcgaggc cacgatcgcg
    24001 gccacgcgcg g
//
LOCUS       DAORUK010000014        23911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_1699_length_23911_cov_18.460262, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000014 DAORUK010000000
VERSION     DAORUK010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 23911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23911
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1699_length_23911_cov_18.460262"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /gene="guaB"
                     /locus_tag="PK818_01860"
     CDS             complement(<1..1178)
                     /gene="guaB"
                     /locus_tag="PK818_01860"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004657166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HQY81511.1"
                     /translation="MLRIQAEALTYDDVSLVPAHSTVLPKDVTLDTRLTRDLRIRLPI
                     VSAAMDTVSEARLAIAMAQLGGISIIHKNMSAEAQAAQVARVKNFEAGVIREPFTVGP
                     DTTIGEVLRLTRARNISGVPVVDGAGQLVGIVTGRDMRFEKKLDDPVRHIMTKKDRLV
                     TVREGASDDEVIGLLHKHRIEKVLVVNDDFALRGLITVKDIQKKSDNPNAAYDSSERL
                     LVGAAVGVGGDTEQRIEALVAAGVDVVIVDTAHGHSQGVIDRVAWTKKRFPSLQVVGG
                     NIVTGDAALALMDAGADAVKVGVGPGSICTTRIVAGVGVPQVTAVAMVAEALQDRIPL
                     IADGGIRYSGDIGKAIVAGASSVMIGGLFAGTEESPGETELFQGRSYKSYRGMGSLGA
                     "
     gene            complement(1332..2195)
                     /gene="folD"
                     /locus_tag="PK818_01865"
     CDS             complement(1332..2195)
                     /gene="folD"
                     /locus_tag="PK818_01865"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HQY81512.1"
                     /translation="MTATILDGKRIADDLLDGLKAQVDARVAAGLPRPGLAVVLVGQD
                     PASQSYVRNKRRAAHKVGIQALDFDLPAGTGEAELLALIDRLNADPTVHGILVQLPLP
                     GIPDASRLIHRIDPRKDVDGFHPENVGHLALRQFGLRPCTPRGITTLLAYTDRPVRGA
                     SATIVGVSNHVGRPMALELLIAGCTVTSCHKFTPPQVLQRHVGEADILVVAVGRPGLI
                     PGDWVKPGAVVIDVGINRLDDGRLVGDVGFTEAATRASWITPVPGGVGPMTVATLMQN
                     TLEAAELADAL"
     gene            2235..2459
                     /locus_tag="PK818_01870"
     CDS             2235..2459
                     /locus_tag="PK818_01870"
                     /inference="COORDINATES: protein motif:HMM:NF018545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HQY81513.1"
                     /translation="MAPRDGAREAVYGMPIAGPRARQQAEATPEPMAAPGACQRWTGT
                     SAFIVLDKSWQGATWQSRANVRNAASGSCT"
     gene            2354..2611
                     /locus_tag="PK818_01875"
     CDS             2354..2611
                     /locus_tag="PK818_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81514.1"
                     /translation="MDWDVGLYRARQELAGGNMAIQGECPKCGKRVLHVNVETIVGIV
                     DGSSKARCISHSCIHCNTVLSVEIDHRAKPRTRSSKPPATA"
     gene            complement(2616..3650)
                     /locus_tag="PK818_01880"
     CDS             complement(2616..3650)
                     /locus_tag="PK818_01880"
                     /inference="COORDINATES: protein motif:HMM:NF021541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2272 domain-containing protein"
                     /protein_id="HQY81515.1"
                     /translation="MRLQRWWTGACLLAFAGSAMAADQCPSLQGRQADPEVATRIAAV
                     ACAEHLRWRRPFIDPAGRLASSVVAEGESRGLDDGGAPWRRVATYWRDAGLLGRTVAA
                     GASDCVHAAGNASYPGLGCRGFVIDSPWSAAFVSWVMQRAGVPGFSHSGGHFEYVRAA
                     RLAPDDSPYQFLEPLSSPPAAGDLLCYVRTGRVHGHEGLARAIDGGATGLAMHCDIVV
                     AANAGGDAKAWLVGGNVQQAVTMRMFNLNATGRFWGLQRRSEGDPECSPDALSACDFN
                     RQDWAVLLKLKSQEQLARLGPVQPPVLLPSATPPAGCCVQCVLGSGVPRCPPTGSLPP
                     QPVPLPSDAD"
     gene            complement(3655..4782)
                     /gene="moeB"
                     /locus_tag="PK818_01885"
     CDS             complement(3655..4782)
                     /gene="moeB"
                     /locus_tag="PK818_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HQY81516.1"
                     /translation="MPIRLLSPSEALARQRAGALLVDVRGDGERALGMAAGAIGIDRE
                     QLQADPARHLADPAAEILLICQLGLRSQLAAQALAACGYRNLASVEGGLQAWQAAGLP
                     MAEPTVDADFGERYARHLRLPGVGVDGQAKLEVARVLLVGAGGLGSPAAFYLAAAGVG
                     HLRIADDDVVDRSNLQRQILHTEARIGLPKVASAAVALSALNPRTSVEAVRERVTAAN
                     VERLLDGVDVVIDGADNFPARYLLNDACVKLGKPLVYGAVHRFEGQASVFDAGRHRGS
                     APCYRCLFPEPPPPEAAPNCAEAGVLGVLPGVIGLLQATEAIKLLLGIGDTLRGRLLQ
                     FDALAMRFRETRLAADPQCPVCAPDRAFNGYADYAAFCAGS"
     gene            complement(4812..5390)
                     /locus_tag="PK818_01890"
     CDS             complement(4812..5390)
                     /locus_tag="PK818_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009602295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HQY81517.1"
                     /translation="MTARGEVTLGILAGGRGSRLGGRDKAWLMRDGVPQVVRIARRFE
                     GECTAVLVSANRDLPRHAAHGLRAVVDRIADRGPLGGIEALASACETPWLLTMPVDLV
                     DPGDGLLRTLASASCGDGAVATDDDGLQPLLALYRVEALRAALADALAAGDYAVQAMQ
                     ARLALPRAGFAGLRFGNLNTPDDLRRAGCDDA"
     gene            complement(5397..6797)
                     /gene="der"
                     /locus_tag="PK818_01895"
     CDS             complement(5397..6797)
                     /gene="der"
                     /locus_tag="PK818_01895"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQY81518.1"
                     /translation="MLPLVALVGRPNVGKSTLFNALTRTRDALVHDQPGVTRDRNYGV
                     CRLAEGRPFVLVDTGGIAGEDEGLAGATARQSRAAAEEADLILFIVDGREGASTVDDD
                     ILRWLRKLSKPTVLVVNKSDGIDLQAALSEFSRYGLGEALPVSSAHRTGIDELLARTL
                     GLLPEEGATETLDDDPKRVRIAFVGRPNVGKSTLVNRILGEERMIASEVPGTTRDSIA
                     VDLERDGRKYRLIDTAGLRRRARVEEAVEKFSVVKTLQAIEQCQVAVLMLDATEGVTD
                     QDATVLGAVLDAGKALVIAINKWDGRTDYERQQAETLVSRKLSFVEWAENVRISALHG
                     SGLRELFRAIHRAHASATKVFSTADVTRAIEAAYVANPPPVVRGHVPKMRFAHPGAES
                     PPTFIVHGTRLKSLSNTYQRYLENFFRKRFKLVGTPIRFIFREGENPYKDKKNVLTDR
                     QVAKKQRLIRRVKRAK"
     gene            complement(6936..8132)
                     /gene="bamB"
                     /locus_tag="PK818_01900"
     CDS             complement(6936..8132)
                     /gene="bamB"
                     /locus_tag="PK818_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HQY81519.1"
                     /translation="MTRAKQRTQFRQAARLLAATALAVALLSGCSSVKNLLDGRGKDH
                     SKDPTPLVELSPSANVSQLWSASLGKGEQQLGIAQRPAIADGKVYAAAVEGGVSAFDL
                     RSGQSLWRYASELQLTGGPGAGDGLVVVGSLEGDVIALDAATGEEKWKSKVANEVLVA
                     PAVGGGMVFVHSNDGRITALDAASGERRWFHSADVPALTVRGSGAITVGPGIIFVGND
                     NGNLTALAMADGAVAWTSPVAQPDGRSELERMADVDGPAVLDNTTLFATSYKNHTVAI
                     DGPSGQLMWDRENGGPRGLGVSNSAVVVTDRDGKVWALDKNTGSSLWQQEGLAYRGTS
                     APAVVGDYAVVGDFEGVLHWLRLNDGAFAARSSLGGAISGQPAVADGVLVVQTTEGQL
                     AAFALQ"
     gene            complement(8132..8776)
                     /locus_tag="PK818_01905"
     CDS             complement(8132..8776)
                     /locus_tag="PK818_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017913736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY81520.1"
                     /translation="MAIDDLLDEHEQSERVRSWIRSNALGLVGGVALGLGAIAGWQWW
                     QGQQLQRGMAVNARYAEVVRNYEAGRLPADKGRAALDTIAKGNQTLATLAALQLAKTQ
                     AEAGKRDDAIATLRGLHDLDPELRVVVRQRLARLLIDAGKGKDALSLLDDERNPAMLD
                     ARGDAQLALGDRAKAQSDYLKALALVDVADPQHRLLTLKLIEAGGMPPHTEDKT"
     gene            complement(8867..9676)
                     /locus_tag="PK818_01910"
     CDS             complement(8867..9676)
                     /locus_tag="PK818_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HQY81521.1"
                     /translation="MTTHRTDTTNDLLAGCGGRLRQAREAAGLSIDDVAARLRMPARI
                     VRSLEAEDWSRLGAPVFVRGQVRSYSRLMGLTTAPMMEALVDVGPVEPSRLVSRTHTP
                     AAQWWAEQLGRRLVYIVLTLFLVVPAWVATRQHLSATVADAAPLDLPVDPAGKAVQRQ
                     LPPAMPRTVVASMAAVPSARPPATEIVIRTRGESWVTVTAVDGSSLERALVPAGSERR
                     YAAAQVAHLTIGNASAVEVESQGRNVDMGAFARANVARFTVSSDGSLVAAD"
     gene            complement(9673..10458)
                     /gene="pilW"
                     /locus_tag="PK818_01915"
     CDS             complement(9673..10458)
                     /gene="pilW"
                     /locus_tag="PK818_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HQY81522.1"
                     /translation="MAVSLLAAGCNRLERLSIVKPTAERGDWTQVAPDYEVSDKGRTA
                     APMAAAQLLASAGAAYRAGKMEQAAQQAQAALKADPAMADAHTLLAAIAIENSDQEAA
                     GRHYQQALAIAPGKGAYANNYGTWLCSNGRAAESLSWFDKAVADPEYGTRAAALANAG
                     TCAHRTGQLGRAEAYWRQALALEPTEMQSLAGMANLEFARARYLEARAFAERWLGIAP
                     SDREALQLASQIELKLGDTDASTRYLQRLQALSPGAINAPRSQ"
     gene            complement(10475..11743)
                     /gene="rlmN"
                     /locus_tag="PK818_01920"
     CDS             complement(10475..11743)
                     /gene="rlmN"
                     /locus_tag="PK818_01920"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006361649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HQY81523.1"
                     /translation="MSDTRTPVIDIALVGDSGVRDPATAAPAAATGGRATADTGGRTN
                     IFDFDRASLERFFEQDLGEKKFRAQQVMKWIHHRYATDFGDMTDLGKALRAKLEQRAV
                     VQAPQVLLDKASEDGTHKWLLGMDPKNAIETVFIPDKGRGTLCVSSQVGCALNCTFCS
                     TATQGFNRNLSTAEIIGQVWVAARHLGNVPHQQRKLTNVVMMGMGEPLANFDNVVRAM
                     SIMRDDLGYGLANKRVTLSTAGMVPMIDKLGEVSDVSLAVSLHAPFDELRNELVPLNR
                     KYPIAELMDACVRYALRKRGTSVTFEYTLMKDVNDQPEHARGLVKLLREFDRRVQMKD
                     AAKVNLIPFNPFPGTRYARPADEAIRAFQKQLNNAGMIAPVRRTRGDDIDAACGQLKG
                     QVMDRTRRSAEHRKHLQDSGIAQGEVPDAA"
     gene            complement(11766..12191)
                     /gene="ndk"
                     /locus_tag="PK818_01925"
     CDS             complement(11766..12191)
                     /gene="ndk"
                     /locus_tag="PK818_01925"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HQY81524.1"
                     /translation="MALERTLSIVKPDAVAKNVIGEIYSRFEQAGLKVVAARMKHLSK
                     QEAEGFYAVHRERPFFGALVNFMISGPVMIQVLEGEGAVLKNRDLMGATNPKDAAAGT
                     IRADFADSIDANAVHGSDSLENAAIEIAYFFPATDVYAR"
     gene            12410..13036
                     /locus_tag="PK818_01930"
     CDS             12410..13036
                     /locus_tag="PK818_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY81525.1"
                     /translation="MSLQHFSTKDRILHAAEELFAQQGFASTSLRQVTSRADVNIAAV
                     NYHFGSKDNLVNEVFRRRMDDMSAERLKALRLAVDSHPGELEPILAAFVEPALAMAQD
                     RHGGGAFIRVIARAYAESNDSLRKFLSDQYGHVLREFAKALSGCLPGLGKEALYWRLD
                     FLSGALTYAMADFGLIKRPSGVSEATHRTRAAKALIRFAAAGFKADEP"
     gene            13058..15430
                     /locus_tag="PK818_01935"
     CDS             13058..15430
                     /locus_tag="PK818_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017158309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HQY81526.1"
                     /translation="MSEKLLVRRAAVLGAGVMGAQIAAQLTNAGVDTVLFDLAAKEGD
                     PNGIVTRAIANLGKLSPAPLASKALAEAITPANYETGLALLEGCDLIVEAIAERMDWK
                     QDLYRRIAPHVPAHAVLASNTSGLGINALADVLPEELRNRFCGVHFFNPPRYMHLAEL
                     IPARTTDPAVLAGLEGFLTSHLGKGVVYAKDTPNFIGNRIGVFSILATIHHTAALGLG
                     FDEVDAVTGPLIGRPKSATYRTSDVVGLDTMAHVIKTMADTLPDDPWHAYFKSPAWLD
                     ALVAKGALGQKTGAGIFRKLGKDIVVLDLEKQDYRIADRAAAPEVVEILKERDPARKF
                     AALRASEHPQARFLWACFRDLFHYSAYHLADIAETARDVDLAIRWGYGWSLGPFETWQ
                     AAGWQQVAGWIAEDIAAGKAMSSAPLPAWATDGRDGVHAAAGSFSPARDALLPRSTLP
                     VYQRQRFPDPVLGERPAPGETVFENDGVRAWHDGDGILVISFKTKLHTVGDTVLDGVP
                     QAIAIAERDFRGAVIWQPKEPFSAGADLSGALGLLQSGDLAGFDAMVANFQATSQRIK
                     YSLVPVVAAVRGLALGGGCEFQMHSARAVAHLESYIGLVEAGVGLLPAGGGLKELAVR
                     ASNAAGPGGDVFAELKKVFETIAMGKVSASAVEAKELGLLRKDDLVSFHAHELLHVAK
                     ATARGMAEAGYRPPLPARRVQVAGDVGIATFRMMLVNMLEGRFISPHDYEIATRIATV
                     LCGGEVERGSLVDEDWLLRLEREHFVALAQMPKTQERIAHMLKTGKPLRN"
     gene            15433..16638
                     /locus_tag="PK818_01940"
     CDS             15433..16638
                     /locus_tag="PK818_01940"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HQY81527.1"
                     /translation="MTKQIQDAYIVAATRTPVGKAPRGVFRNTRPDDMLAHVLRSVVA
                     QAPGIDVNRIDDAIIGCAMPEGEQGMNVARIGVLLAGLPDTVAAQTINRFCSSGLQAV
                     ALAADQIRLGNADLMLAGGTESMSMVPMMGNKVALAPSVFRDDHVAIAYGMGITAEKV
                     AEEWKVSREEQDAFAYASHQKAIAAIAAGEFRDEISPYDVVARMPDLAGNAVRLKQLR
                     VENDEGPRLDTTVEGLAKLRPVFRNGQFGGSVTAGNSSQMSDGAGAVLLASEQAIKDY
                     GLTPLARFVSFSVAGVRPEVMGIGPIAAIPKALKQAGLTRDQLDWIELNEAFAAQALA
                     VIKDCGLDPAKVNPLGGAIALGHPLGATGAVRTATIVHGLRRRQQKYGMVTMCIGTGM
                     GAAGIFEGL"
     gene            complement(16781..17707)
                     /gene="galU"
                     /locus_tag="PK818_01945"
     CDS             complement(16781..17707)
                     /gene="galU"
                     /locus_tag="PK818_01945"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HQY81528.1"
                     /translation="MQSPKPHRRVRIAVFPVAGLGTRFLPATKTVPKEMLPIVDKPLI
                     QYAVDEAVEAGCDTLVFITNRYKHAVADYFDKAYELEQKLEKAGKAEQLELIRNVLPP
                     GVRAVFVTQAEALGLGHAVLCAKPVVGDQPFAVLLPDDLIWTRQSVAGGAGALKQMAD
                     HAERTGGSVIAVQDVPPEQTKSYGIVATEGFDGQAGRITRIVEKPKPEDAPSNLAVVG
                     RYILNPAIFDLLETTQQGAGGEIQLTDAIAQLLPLEQVDAFRFHGTRFDCGTHLGLIE
                     ATVRYALDHELLSDAARKLMQNALDEMGVVET"
     gene            complement(17721..19631)
                     /locus_tag="PK818_01950"
     CDS             complement(17721..19631)
                     /locus_tag="PK818_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HQY81529.1"
                     /translation="MTSWRDRFKYGLPRLAVMLHDLAMVWVCWQGLHYIRYALQPTPL
                     PLEPLSSTVLIVMVAQGLVSWRVGLYRGLWRFASVPDLLNIFKASVLGLLAVVAGLFF
                     YSRLDLVSRAALLLYPFVLTMLLGAPRLIFRAWKDQRLLRSDETAERVLVLGAGQAGE
                     AVVRDLRRIGRYQPVGFVDDAAALRGTRLQGLPILGRVGDVARIARETGVQLLVIAMP
                     SLDAIAMRRVVGICEESGVPFRTVPRLSDVLDGRSLPGQLKEVAIEDLLGRQPLSPDW
                     KAIRNWLGARSVLVTGAGGSIGSELCRQCARHGAKRIALLEIDELALLTVEADLRRDF
                     PGIECLPVLGDAGDPAVIAHALAIAEPEAVFHAAAYKQVPLLQRQLREAVRNNVLATD
                     VVARASRDAGVGTFVLISTDKAVDPVNVLGATKRLAEMLCQALVDPRSTRFVTVRFGN
                     VLDSAGSVVPLFRDQIRRGGPVTVTDPEVTRYFMTIPEACQLILQASAIGAQQAVYTL
                     DMGEPVSIRMLAEQMIRLAGKQPGKDIAIEYTGLRPGEKLHETLFHVDERYSATIHPK
                     ILQALPRSVSVERVDPALQALREATASYDLERLAASLRAAVPEFNPASEADSARASTT
                     ILNFPARRARPN"
     gene            complement(19628..20650)
                     /locus_tag="PK818_01955"
     CDS             complement(19628..20650)
                     /locus_tag="PK818_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HQY81530.1"
                     /translation="MPWSEPAIPTPVVLLAAALAALLVSMLGSRWAILHARREGLVDQ
                     PGERRSHALPTPRGGGIGIVMACLAALLALAGTQPDASAWLWRACGLVLVAAIGWWDD
                     HRPLPAWPRLLAHAAGAACLAWALHLEGAAPATTIAAFLLALVLVNAWNFMDGINGLA
                     ASQALLCATGFAVVLGDGWRLLALVLAAACLGFLPFNFPRARVFLGDVGSGGLGYLVA
                     ALLAAGFAARPMAWWPLLLLPPLAMLADTGLTLLWRIRRRERWWQAHVQHAFQRWSRH
                     RGHAWVCAAYAVWTFAAVALMLAALHWPARIALVVALATLPASAWAWRWLHRRYQKNN
                     TEGFGT"
     gene            complement(20637..21815)
                     /gene="lapB"
                     /locus_tag="PK818_01960"
     CDS             complement(20637..21815)
                     /gene="lapB"
                     /locus_tag="PK818_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HQY81531.1"
                     /translation="MGNFDVWIWFVVLVPLAALAGWVTGRRGGERHRDSQVSKLSTTY
                     FRGLNYLLNEQPDKAIELFLHIAELDKDTFETQVALGHLFRRRGEVDRAIRLHQALVQ
                     RPDLNDAQKVQALLALGEDYMRSGLLDRAETVFSDLARIDQRAPQALRHLISIYQAER
                     DWEKAIENASRYEAATAEPMGKLVAQFECELADRQRSAGEIDAARAAIARAYAADSTS
                     VRAGISEGRIEAEAGNPAGAVRAFERAARHDPDYLPVILPALLESYGKVGERTGARAF
                     LAEMSEHYRGISPVLALTKMVEAEEGVGAARRYLGQQLKDRPSVRGEAALIDLTLAEN
                     ADAEATLHDLKHITDQLLVRNPSYRCNRCGFGARSHHWQCPSCKEWGTVKPLLNYAVV
                     "
     gene            complement(21817..22086)
                     /locus_tag="PK818_01965"
     CDS             complement(21817..22086)
                     /locus_tag="PK818_01965"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HQY81532.1"
                     /translation="MRLIRLIIAVTCILLGAIVGALNTQPVLLDIGFASVRASLGLSV
                     LVALLLGVVVGGGILAIGVVAPLRRRLRRAEQARPVAAGSARAET"
     gene            complement(22131..22433)
                     /locus_tag="PK818_01970"
     CDS             complement(22131..22433)
                     /locus_tag="PK818_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011408447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HQY81533.1"
                     /translation="MTKSELIELLARRQPHLKADDVDLSVKSLLQMMGAALAGGDRIE
                     VRGFGSFSLHFRPPRTGRNPKTGDAVALPGKHVPHFKPGKELRERVTTVVPLPMDD"
     gene            complement(22517..>23911)
                     /gene="rpsA"
                     /locus_tag="PK818_01975"
     CDS             complement(22517..>23911)
                     /gene="rpsA"
                     /locus_tag="PK818_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HQY81534.1"
                     /translation="LEEALEANATIVGRISGKVKGGFTVDIKDVRAFLPGSLVDVRPV
                     RDPVYLEGKELEFKLIKLDRKRNNVVVSRRAVVESEHSEEREQLLEKLVEGAVLKGVV
                     KNLTDYGAFVDLGGIDGLLHITDMAWKRVRHPSEVVEVGAELDVRVLKYDKERNRVSL
                     GLKQLGEDPWDNIARRYPANSRVFGKVSNVTDYGAFVEIEPGVEGLVHVSEMDWTNKN
                     VNPAKVVQVGDEVQVMVLDVDEERRRISLGIKQVTSNPWETFAAIHKKNDKVSGQIKS
                     ITDFGIFIGLDGGIDGLIHLSDISWNSTGEDIARNYKKGDTLEAVVLAVDPERERISL
                     GVKQMEQDPLGQFMATNPKGTKVTGTVKEVDAKGATIDLGDGVEGYVFARDVADERVE
                     DASHYLKVGQQVEAKFVGMDRKGRSLQLSIKGKDDAEAAEAMAEYQKASADAASGTTK
                     LGALLREQLNSKSE"
BASE COUNT         3648 a         8433 c         8145 g         3685 t
ORIGIN      
        1 atcgcgccca gcgagcccat gccgcggtag ctcttgtagc tgcggccctg gaacaattcg
       61 gtctcgcccg ggctttcctc ggtgccggcg aacaggccgc cgatcatcac gctggacgcg
      121 ccggcgacga tcgccttgcc gatatcgccc gagtagcgga taccgccgtc cgcgatcagc
      181 ggaatgcgat cctgcagcgc ctcggccacc atcgccaccg ccgtgacctg cggcacgccg
      241 acgccggcga cgatgcgcgt ggtgcagatc gagccggggc caacaccgac cttcaccgca
      301 tccgcgcccg cgtccatcag cgccagcgcg gcatcgccgg tgacgatgtt gccgcccacg
      361 acctgcagcg acgggaagcg cttcttggtc catgccacgc ggtcgatcac gccctgcgaa
      421 tggccgtgcg cggtatcgac gatcaccacg tccacgcctg ccgcgaccag tgcctcgatg
      481 cgctgctcgg tgtcgccgcc aacgccgacc gcggcgccga ccagcaggcg ttcgctgctg
      541 tcgtaggcgg cgttcgggtt gtcggacttc ttctggatgt ccttgaccgt gatcaggccg
      601 cgcaacgcga agtcgtcgtt caccaccagc accttctcga tccggtgctt gtgcagcagg
      661 ccgatcacct cgtcgtccga ggcgccttcc ctgaccgtga ccaggcggtc cttcttggtc
      721 atgatgtggc gcaccggatc gtcgagcttc ttctcgaagc gcatgtcgcg gccggtgacg
      781 atgccgacca gctggcctgc gccgtccacc accggcaccc cggagatgtt gcgggcgcgg
      841 gtcaggcgca ggacttcgcc gatggtggtg tcgggcccga ctgtgaacgg ctcgcggatg
      901 acgccggcct cgaagttctt gacccgcgcg acctgcgcgg cctgcgcttc tgccgacatg
      961 ttcttgtgga tgatcgagat gccgccgagc tgggccatgg cgatcgccag gcgggcttcg
     1021 ctgacggtgt ccatcgccgc ggagacgatc ggcaaccgga tacgcaggtc gcgggtcagg
     1081 cgggtgtcga gggtgacgtc cttgggcagg acggtcgaat gcgcgggaac gagggaaacg
     1141 tcgtcgtagg tgagcgcttc agcctggatg cggagcatgg ggtgtccggt ggcttgtgaa
     1201 gcgcgtcatt gtaaccggtt tggtggctcg gccgctcgcg cgaggcgaga gcggcgggca
     1261 ttcgctgcgc ggctcgaaat gccgctgcgc gaacgcccgc cgctcccgcc ttcgccccac
     1321 gacatcgccg gtcaaagtgc gtctgcgagt tccgcggctt cgagcgtgtt ctgcatcagg
     1381 gtggccacgg tcatcgggcc gacgccgccg ggcaccgggg tgatccagct ggcgcgggtg
     1441 gcggcttcgg tgaaaccgac atcgccgacc aggcggccat cgtctaggcg gttgatgccg
     1501 acgtcgatca ccaccgcgcc cggcttcacc cagtcgccgg gaatcagccc gggacgccca
     1561 accgcgacca ccaggatgtc ggcttcgcca acgtgccgct gcagcacctg cggcggggtg
     1621 aacttgtggc acgaggtcac cgtgcagccg gcgatcagca gttccagcgc catcggccgg
     1681 ccgacgtggt tggacacacc cacgatcgtg gcgctggcgc cgcgcaccgg gcggtcggtg
     1741 taggccagca gggtggtgat gccgcgcggg gtacacgggc gcaggccgaa ctggcgcagc
     1801 gcgaggtggc cgacattctc cggatggaag ccatcgacgt ccttgcgcgg gtcgatgcgg
     1861 tggatcagcc ggctggcgtc ggggatgccc ggcagcggca actgcaccag gatgccgtgc
     1921 accgtcgggt ccgcattgag cctgtcgatc agcgccagca gctcggcctc gccggtgccg
     1981 gcgggcaggt cgaaatcaag ggcctggatg ccgaccttgt gcgccgcgcg gcgcttgttg
     2041 cgcacgtagg actgcgaggc cgggtcctgc ccgaccagca ccaccgccag ccccggccgc
     2101 ggcagcccgg cggccacgcg ggcatcgacc tgggccttca ggccatccag caggtcgtcg
     2161 gcgatgcgct tgccatcgag aatcgttgcg gtcatgcgtg gcgatgcggt tgggtcggtc
     2221 gcgcattatc cccgatcgca ccccgcgacg gcgcccgcga agcggtgtac ggtatgccga
     2281 tcgcagggcc cagggcgcgg caacaggccg aagccacgcc ggagccaatg gccgcccccg
     2341 gggcttgcca gcgttggact gggacgtcgg cctttatcgt gctcgacaag agctggcagg
     2401 gggcaacatg gcaatccagg gcgaatgtcc gaaatgcggc aagcgggtcc tgcacgtgaa
     2461 cgtggaaacc atcgtcggca tcgtggatgg ctccagcaag gcacgctgca tcagccacag
     2521 ctgcatccac tgcaacaccg tgctgagcgt ggagatcgac caccgcgcca agccgcgcac
     2581 acgcagcagc aagccgccag cgacagcctg aggtgtcagt ccgcgtcgct cggtagcggc
     2641 accggttgcg gtggcaggct gcccgtgggc gggcaacgcg ggaccccgga cccgagcacg
     2701 cactgcacgc agcaccccgc tggcggggtt gcggacggca acagcacggg tggctgcacc
     2761 ggcccaagcc tggccagctg ttcctgcgac ttgagcttga gcagcaccgc ccagtcctgg
     2821 cggttgaaat cgcaggccga aagcgcgtcc ggcgaacatt cgggatcgcc ttcgctgcgc
     2881 cgctgcaggc cccagaagcg gccggtggca ttcaggttga acatgcgcat ggtgaccgcc
     2941 tgctgcacgt tgccgccgac cagccaggcc ttggcatcgc cgccggcgtt ggccgcgacc
     3001 acgatgtcgc aatgcatggc aagaccggtg gcaccgccgt cgatcgccct ggccaggcct
     3061 tcgtggccat gcacgcggcc ggtgcgcacg tagcacagca ggtcgccggc cgccggaggc
     3121 gaagacagcg gttccaggaa ctggtacggg ctgtcgtcgg gggccagccg ggcggcgcgg
     3181 acgtattcga aatggccacc ggaatggctg aacccgggga cgcctgcgcg ctgcatcacc
     3241 cacgacacga acgcggcaga ccacgggctg tcgatcacga agccgcggca gcccaggccg
     3301 ggataggacg cgttgcctgc cgcatgcacg cagtcgctgg caccggccgc gacggtccgt
     3361 ccgagcaggc cggcatcgcg ccagtacgtc gcgacccgcc gccacggcgc accgccatcg
     3421 tcgaggccgc ggctctcgcc ttccgccacc acgctgctgg cgaggcggcc cgcgggatcg
     3481 atgaagggac gccgccagcg caggtgttcg gcgcaggcga ccgcggcgat gcgggtggcg
     3541 acctcgggat ctgcctgccg gccttgcagc gacgggcact ggtcggcggc catggcgctg
     3601 cccgcgaacg ccagcaggca ggcgccggtc caccaccgtt gcagtcgcat cacctcaact
     3661 cccggcgcag aacgccgcgt aatccgcata tccattgaat gcgcgatccg gcgcgcaaac
     3721 cggacactgc gggtcggcgg ccagccgggt ctcgcggaat cgcatcgcca acgcatcgaa
     3781 ctgcagcagg cggccgcgca gggtatcgcc gatgccgagc agcaacttga tcgcctcggt
     3841 ggcctgcagc agcccgatca cccccggcag caccccgagc acgccggcct cggcgcaatt
     3901 gggcgcggcc tccggcggcg gcggctccgg gaacaggcag cggtagcacg gcgcgctgcc
     3961 gcgatggcgg ccggcatcga acacgctggc ctggccttcg aaccgatgca ccgctccgta
     4021 caccagcggc ttgccgagct tcacgcaggc atcgttgagc aggtagcgcg cggggaaatt
     4081 gtcggcgccg tcgatgacca cgtcgacgcc gtcgagcagg cgctcgacat tcgccgcggt
     4141 cacccgttcg cgcacggctt cgaccgacgt gcgcggattg agcgcggaca gcgcgaccgc
     4201 cgccgacgcg accttcggca agccgatgcg ggcctcggtg tgcaggatct gccgttgcag
     4261 gttgctgcgg tcgaccacgt cgtcgtcggc gatgcgcagg tggccgacgc ccgccgccgc
     4321 gagatagaac gccgccggcg atcccaggcc accggcgccc accagcagca cccgcgccac
     4381 ttccagtttc gcctgaccgt ccacgccgac cccgggcaag cgcaggtggc gcgcatagcg
     4441 ttcgccgaaa tcggcatcga cggtgggctc cgccatcggc aggccggccg cctgccacgc
     4501 ctgcagcccg ccctcgaccg aggccaggtt gcgatagccg caggccgcca gggcctgcgc
     4561 cgccagttgc gagcgcaggc ccagctggca gatcagcagg atctccgccg cgggatcggc
     4621 aaggtggcgc gcgggatctg cctgcaactg ctcgcggtcg atcccgatcg cgcccgcggc
     4681 catcccgagc gcacgctcgc cgtccccgcg cacgtcgacc agcaacgcac cggcacgctg
     4741 gcgggcaagg gcttcgctcg gactcaggag gcggatcggc atgcctcgat tatccccgat
     4801 cggcgggcgc ctcaggcgtc gtcgcaaccg gcgcggcgca ggtcgtcggg cgtgttgagg
     4861 ttgccgaagc gcagcccggc gaatccggcg cgcggcagcg cgagccgcgc ctgcatcgcc
     4921 tgcaccgcgt aatcgcccgc ggccagcgcg tcggccaatg ccgcacgcag ggcctcgacc
     4981 cggtacagcg cgagcagcgg ctgcaggccg tcgtcatccg tggccaccgc gccatcgccg
     5041 caactggcgc tcgccagcgt gcgcaacagg ccatcgccgg gatcgacgag atccaccggc
     5101 atggtcagca gccacggcgt ttcacaggcc gatgccagcg cctcgatgcc gccgagtgga
     5161 cccctgtcgg cgatgcggtc gacgaccgcg cgcagtccgt gcgcggcatg gcgcggcagg
     5221 tcccggttcg cgctgaccag caccgcggtg cactcgccct cgaagcgacg ggcgatgcgc
     5281 accacctgcg gcacgccatc acgcatcagc caggccttgt cgcgtccgcc cagccggctg
     5341 ccgcggccgc cggcaaggat gcccagggtc acctcgcctc gggccgtcat cgcctgctac
     5401 ttggcgcgct tgacgcggcg gatcagccgc tgcttcttcg cgacctggcg atcggtgagg
     5461 acgttcttct tgtccttgta cggattttcg ccttcccgga agatgaagcg gatcggcgtg
     5521 ccgaccagct tgaagcgctt gcggaagaag ttctcgaggt agcgctggta ggtgttcgac
     5581 agcgacttca gccgcgtgcc atgcacgatg aaggtcggtg ggctttccgc ccccgggtgc
     5641 gcgaagcgca tcttcggcac atgaccgcgc acgaccggcg gcggattcgc cacgtaggcc
     5701 gcttcgatcg cccgggtcac gtcggcggtg ctgaacacct tggtcgccga cgcgtgcgcg
     5761 cggtggatcg cgcggaacag ttcgcgcagg ccggaaccgt gcagtgccga aatgcgcacg
     5821 ttctccgccc attccacgaa gctgagcttg cgcgacacca gggtctcggc ctgctggcgt
     5881 tcgtagtccg tgcgcccgtc ccacttgttg atcgcgatga ccagcgcctt gccggcatcc
     5941 agcaccgcgc cgagcacggt cgcgtcctgg tcggtgacgc cttcggtggc gtccagcatc
     6001 agcaccgcga cctggcattg ctcgatcgcc tgcagggtct tcaccaccga gaatttctcg
     6061 accgcctcct ccaccctggc gcggcggcgc aggccggcgg tgtcgatcag gcggtacttg
     6121 cggccgtcgc gctcgaggtc gaccgcgatc gaatcgcggg tggtgcccgg cacctcggag
     6181 gcgatcatcc gttcctcgcc gaggatgcgg ttgaccaggg tcgacttgcc cacgttcggg
     6241 cggccgacga aggcgatgcg cacgcgcttc ggatcgtcgt ccagggtctc ggtcgcgccc
     6301 tcttccggca gcaggcccag ggtcctggcc agcagttcgt cgatgccggt gcggtgcgcg
     6361 gacgacaccg gcaacgcctc gccgaggccg tagcgggaaa attccgacag cgcggcctgc
     6421 aggtcgatcc cgtccgactt gttgaccacc agcacggtcg gcttcgacag cttgcgcagc
     6481 cagcgcagga tgtcgtcgtc caccgtggag gcgccctcgc ggccatcgac gatgaacagg
     6541 atcaggtcgg cctcttccgc ggcggcgcgc gactgccgcg cggtggcacc ggccaggccc
     6601 tcgtcctcgc cggcgatgcc gccggtgtcg accagcacga acggccgccc ttcggcgagg
     6661 cggcagacgc cgtagttgcg gtcgcgggtg acgcccggct ggtcgtgcac cagcgcatcg
     6721 cgggtgcggg tcaacgcatt gaacagcgtg gacttgccga cgttggggcg cccgaccagg
     6781 gcgacgaggg gcagcatgga tcaacctttg cgcggttcgc gcgtggaaaa caacgcggcc
     6841 aacgcgcgca ggcggcgcgt tgcccgaaaa gcagacagcc ccggcagact agccggggcc
     6901 gtgcatgtgc gccagcccgc gggccggcgc cggacttact gcagggcgaa cgccgcgagc
     6961 tggccttcgg tggtctgcac caccagcacg ccatcggcga ccgcgggctg gccgctgatc
     7021 gcaccaccca ggctggaacg cgcggcgaac gcgccgtcgt tcaggcgcag ccagtgcagc
     7081 acgccctcga agtcgccgac caccgcgtag tcgcccacga ccgccggtgc actggtgccg
     7141 cggtaggcca ggccttcctg ctgccacagg ctgctgccgg tgttcttgtc gagcgcccag
     7201 accttgccgt cgcggtcggt cacgaccacc gccgaattgc tgacgccaag cccgcgcggg
     7261 ccaccgttct cgcgatccca catcagctgg ccgctgggac cgtcgatcgc caccgtgtgg
     7321 ttcttgtaac tggtcgcgaa cagggtggtg ttgtccagca ccgccgggcc gtccacgtcg
     7381 gccatccgct cgagttcgct gcggccgtcc ggctgcgcca ccggcgacgt ccaggccacg
     7441 gcgccatcgg ccatcgccag cgcggtcagg ttgccgttgt cgttgccgac gaagatgatc
     7501 ccgggcccga cggtgatcgc gccgctgccg cgcacggtga gggccggcac gtcggcgctg
     7561 tggaaccagc ggcgttcgcc gcttgcggca tccagcgcgg tgatgcggcc gtcgttcgaa
     7621 tgcacgaaca ccatgccgcc gccaaccgca ggcgcgacca gcacttcgtt ggcgaccttc
     7681 gacttccact tctcctcgcc ggtggcggcg tcaagcgcga tcacgtcgcc ctcgaggctg
     7741 ccgaccacga ccaggccatc gcccgcgccg gggccaccgg tcaactgcaa ttcggaggca
     7801 tagcgccaca gggactgccc gctgcgcagg tcgaacgcgg acacgccgcc ctcgaccgcg
     7861 gcggcataca cctttccatc ggcgatcgcc ggacgctggg cgatgcccag ctgctgttcg
     7921 cccttgccga gcgatgcgct ccacaactgg ctcacgttgg cgctggggct gagctccacc
     7981 agcggggtcg gatccttgga gtggtccttg ccgcggccat ccagcaggtt cttcacgctc
     8041 gaacagcccg acagcagggc gacggcgagc gcggtggcgg caagcaggcg tgcggcctgg
     8101 cggaactggg tgcgttgctt ggcccgggtc atcaggtctt gtcctcggta tgcggcggca
     8161 tgccgccggc ctcgatcagc ttcagggtca gcaggcgatg ctggggatcg gcgacgtcga
     8221 ccagggcgag cgccttcagg tagtcggact gcgccttggc acggtcgccc agcgcgagct
     8281 gcgcatcccc acgcgcgtcg agcatggccg ggttgcgttc gtcgtcgagc agggacagcg
     8341 cgtccttgcc cttgccggcg tcgatcagca gccgcgccag gcgctggcgc acgaccacgc
     8401 gcaattcggg atcgaggtcg tgcagtccgc gcagggtggc gatggcgtca tcccgcttgc
     8461 cggcctcggc ctgggtcttg gccagttgca gtgccgccag cgtggccagg gtctggttgc
     8521 ccttggcgat cgtgtccagc gccgcgcggc ccttgtccgc gggcagccgg ccggcttcgt
     8581 agttcctcac gacctccgcg tagcgggcat tgaccgccat cccgcgctgc aattgctggc
     8641 cttgccacca ctgccagccg gcgatcgcgc cgaggccaag ggccacgccg ccgaccaggc
     8701 ccagtgcatt gctgcggatc caggaacgga ctcgttcgct ctgttcgtgc tcgtcgagca
     8761 ggtcatcgat cgccatgctg tctctcgccc gcgttcgcta cgcgggtctc catgtgtagg
     8821 gtgtggaatc gcccgccggg ttgagccggc gaccgcgacg gccgcttcaa tccgccgcga
     8881 cgagcgaacc gtcggaggat accgtaaagc gcgcgacgtt cgcgcgggcg aaggcgccca
     8941 tgtccacgtt gcgcccctgg ctctcgactt ccaccgcgga ggcgttgccg atggtcaggt
     9001 gcgcgacctg agcggcggca taacggcgct cgctgcctgc ggggaccagt gccctctcga
     9061 ggctgctgcc atcgaccgcg gtcaccgtga cccagctttc gccgcgggtg cggatgacga
     9121 tctcggttgc tggcggacgc gccgacggca ccgcggccat cgacgcaacc acggtgcgcg
     9181 gcatcgccgg tggcagctgg cgctgcaccg ccttgcccgc gggatccacc ggcaggtcca
     9241 gcggcgcggc atcggcaacg gtggcggaca ggtgctggcg ggtggcgacc caggccggca
     9301 ccaccaggaa cagggtcagc acgatgtaca ccagccggcg gccgagctgc tcggcccacc
     9361 actgcgcggc cggcgtgtgg gtccggctga ccaggcgcga gggttcgacc ggccccacgt
     9421 ccaccagcgc ttccatcatc ggcgcggtgg tgaggcccat cagccgcgag tagctgcgca
     9481 cctgcccgcg gacgaagacc ggcgccccca gccgagacca gtcctcggct tccagcgaac
     9541 gcacgatccg cgccggcatg cgcagcctgg cggcgacgtc gtcgatgctg aggcccgcgg
     9601 cttcgcgcgc ctgcctcagg cggccgccac aaccggccag caggtcgttc gtcgtatctg
     9661 tgcggtgggt ggtcattgcg aacggggtgc gttgatggcg ccgggggaca acgcctgcaa
     9721 tcgttgaagg tagcgtgtgg acgcgtcagt atcgccgagt ttgagctcga tctgtgacgc
     9781 cagttgcaaa gcctcgcgat ccgagggggc aatgccgagc cagcgctcgg cgaatgcccg
     9841 cgcctcgagg tagcgcgcgc gcgcgaattc caggttggcc atgccggcaa gcgactgcat
     9901 ttcggtgggt tccagcgcca gcgcctgccg ccagtaggcc tccgcgcggc ccagctgccc
     9961 ggtccggtgc gcgcaggtac cggcgttggc cagcgcggcg gcgcgggtgc cgtattccgg
    10021 atccgccacc gccttgtcga accaggacag ggattcggcg gcgcgcccgt tgctgcacag
    10081 ccaggtgccg tagttgttgg cgtaggcgcc ctttccaggg gcgatcgcca gcgcctgctg
    10141 gtagtgcctg ccggcggcct cctggtcgga attctcgatc gcgatggcgg caagcagggt
    10201 atgcgcgtcc gccatcgcgg ggtcggcctt cagcgccgcc tgtgcctgct gcgcggcctg
    10261 ctccatcttg cccgcacggt aggccgcgcc ggccgaggcc agcaattgcg cggcggccat
    10321 cggcgccgcc gtgcggccct tgtcgctgac ctcgtaatcc ggcgcgacct gggtccagtc
    10381 gccgcgttcg gcggtcggct tgacgatgct caaccgctcc agccggttgc agcccgcggc
    10441 gagcagggaa accgccatgc acagcaggag cgcctcacgc cgcatccggc acctcgcctt
    10501 gcgcgatccc gctgtcctgc aggtgcttgc ggtgctccgc cgaacggcgg gtgcgatcca
    10561 tcacctgccc cttcagctgg ccgcaggcgg catcgatgtc gtcgccgcgg gtgcgccgca
    10621 ccggcgcgat catgccggca ttgttgagct gcttctggaa cgcgcggatc gcctcgtcgg
    10681 ccggccgcgc gtagcgggtg ccggggaacg ggttgaacgg gatcaggttg accttggccg
    10741 catccttcat ctgcacccgg cggtcgaact cgcgcagcaa cttgaccagg ccgcgcgcat
    10801 gctccggctg gtcgttgacg tccttcatca gcgtgtattc gaaggtcacc gaggtgccac
    10861 gcttgcgcag cgcatagcgc acgcaggcat ccatcagctc ggcgatcggg tacttccggt
    10921 tgagcggcac cagctcattg cgcagttcgt cgaacggcgc atgcagggac accgccagcg
    10981 agacatcgct gacctcgccc agcttgtcga tcatcggcac catgccggcg gtggacaggg
    11041 tgacccgctt gttggccagt ccatagccga ggtcgtcgcg catgatgctc atcgcgcgca
    11101 cgacgttgtc gaaattggcc agcggctcgc ccatgcccat catcaccacg ttggtgagct
    11161 tgcgctgctg gtgcggcacg ttgccaaggt ggcgcgccgc cacccacacc tggccgatga
    11221 tctcggcggt ggagaggttg cggttgaagc cctgggtggc ggtggagcag aacgtgcaat
    11281 tcagggcgca gcccacctgc gagctgacgc agagggtgcc gcggcccttg tccgggatga
    11341 acacggtctc gatcgcgttc ttcggatcca tgccgagcag ccacttgtgg gtgccgtctt
    11401 ccgaagcctt gtccagcagc acctgcggtg cctgcaccac cgcccgttgc tcgagcttgg
    11461 cgcgcagcgc cttgccgagg tcggtcatgt cgccgaaatc ggtcgcgtag cggtggtgga
    11521 tccacttcat cacctgctgc gcgcggaact tcttttcgcc gagatcctgc tcgaagaagc
    11581 gctcgagcga agcgcggtcg aagtcgaaga tgttggtgcg cccgcccgtg tcggccgtcg
    11641 ccctgccgcc ggtggcggcg gccggagccg ccgtcgccgg gtcgcgcacg ccggaatcgc
    11701 ccacgagggc aatatcgatg accggggtgc gggtgtcgct catcggcagc gcgaccgatc
    11761 cgggctcagc gcgcgtagac gtcggtcgcc gggaagaagt aggcgatctc gatcgcggcg
    11821 ttctccagcg aatccgagcc gtgcaccgcg ttggcgtcga tgctgtcggc gaagtcggcg
    11881 cggatggtgc cggccgcggc atccttcggg ttggtggcgc ccatcaggtc gcggttcttc
    11941 agcaccgcgc cctcgccttc cagcacctgg atcatcaccg gtccggagat catgaagttg
    12001 accagcgcac cgaagaacgg gcgctcgcgg tgcacggcat agaagccttc cgcttcctgc
    12061 ttggacaggt gcttcatgcg cgcggcgacc accttcaggc cggcctgctc gaagcgggaa
    12121 tagatttcgc cgatcacgtt cttggcaacg gcatcgggct tgacgatcga aagagtgcgc
    12181 tccagcgcca tgggattact ccagtgaggc gggcggcaac gtggtcgcgg cccgaagata
    12241 acagaaaaac ccgcgtactg gcgcgggttt ggccgatatg gctgcggcgg attctaaccg
    12301 attgccaccc cgctccgcac ctgttttcga agttgatggt cgcctgccgg ttgacgcatt
    12361 ccccgattgc gccctaagat caatcaagcg tttgattcag ggaccgcgga tgagcctgca
    12421 gcacttttcc accaaggacc ggatcctgca cgcggcggaa gagcttttcg cccagcaggg
    12481 cttcgcctcg acctcgctgc ggcaggtcac cagccgcgcc gacgtcaaca tcgcggcggt
    12541 caactaccac ttcggcagca aggacaacct ggtcaacgag gtgttccgcc ggcgcatgga
    12601 cgacatgagc gccgaacgcc tgaaagccct gcgcctggcg gttgacagcc atcccggcga
    12661 gcttgagccg atccttgccg cgttcgtgga gccggcactg gcgatggccc aggaccgcca
    12721 cggcggaggc gccttcatcc gcgtgatcgc ccgcgcctac gccgaaagca acgacagcct
    12781 gcgcaagttc ctgtccgacc agtacggcca cgtcctgcgc gagttcgcca aggcgctttc
    12841 cgggtgcctg cccgggctcg gcaaggaagc gctgtactgg cggctggact tcctgtccgg
    12901 cgcactgacc tacgcgatgg ccgatttcgg cctgatcaag cgtccatccg gggtgtccga
    12961 ggcgacccat cgcacgcgcg ccgcaaaggc cctgattcgt tttgccgccg ccggcttcaa
    13021 ggcggacgaa ccctgattac tcaaggagtt gcatcctatg tctgagaaac tacttgtacg
    13081 ccgcgccgcg gtccttggcg ccggcgtgat gggcgcgcag atcgccgccc agctgaccaa
    13141 tgccggggtc gacaccgtgt tgttcgacct cgccgcgaag gaaggcgacc ccaacgggat
    13201 cgtgacccgc gcgatcgcca acctcggcaa gctgtcgccg gcgccactgg ccagcaaggc
    13261 gctggccgag gcgatcaccc cggcgaacta cgagaccggc ctggcgctgc tcgaaggttg
    13321 cgacctgatc gtggaggcaa tcgccgagcg gatggactgg aaacaggacc tgtaccgccg
    13381 gatcgccccc catgtacccg cccacgcagt gctggccagc aacacatccg gcttgggaat
    13441 caatgccttg gctgatgttc ttcctgaaga acttcgaaac cggttctgcg gcgtgcactt
    13501 cttcaacccg ccccgttaca tgcacctggc cgaactcatc ccggcccgca ccaccgaccc
    13561 cgcggtgctg gccggcctgg aaggcttcct gaccagccat ctcggcaagg gcgtggtgta
    13621 cgccaaggac accccgaact tcatcggcaa ccgcatcggc gtgttctcga tcctggccac
    13681 catccaccac actgcggcgc tcggcctggg cttcgatgag gtcgatgcgg tcaccggccc
    13741 gctgatcggg cgaccgaagt cggcgaccta ccgcacctcc gacgtggtcg gcctggacac
    13801 catggcgcac gtcatcaaga ccatggccga caccctgccc gacgatccgt ggcacgccta
    13861 cttcaagtcg ccggcatggc tggacgccct ggtcgccaag ggggcgctgg gccagaagac
    13921 cggggccggc atcttccgca agctcggcaa ggacatcgtg gtcctcgacc tggagaagca
    13981 ggattaccgc atcgccgacc gcgccgccgc gccggaggtg gtggagatcc tgaaggagcg
    14041 cgacccggcc aggaagttcg ccgcgctgcg cgccagcgag cacccgcagg cccgcttcct
    14101 gtgggcctgc ttccgcgacc tgttccacta cagcgcctac caccttgcgg acatcgccga
    14161 gaccgcgcgc gacgtcgacc tcgccatccg ctggggctac ggctggtcgc tgggcccgtt
    14221 cgaaacctgg caggcggccg gctggcagca ggtggccggc tggatcgccg aggacatcgc
    14281 cgccggcaag gccatgagtt cggcgccgct gccggcctgg gccacggatg gccgcgacgg
    14341 cgtgcatgcg gccgcgggca gcttcagccc ggcccgcgac gcgctgctgc cacgctccac
    14401 cctgccggtc taccagcgcc agcgcttccc ggacccggtg ctcggcgagc gccccgcccc
    14461 cggcgaaacc gtcttcgaaa acgacggcgt gcgcgcctgg cacgatggcg acgggatcct
    14521 ggtgatctcg ttcaagacca agctgcacac ggtcggcgac accgtgctgg atggcgtccc
    14581 gcaggcgatc gcgatcgccg agcgcgactt ccgcggcgcc gtgatctggc agccgaagga
    14641 accgttctcc gctggcgccg acctgtccgg cgcgcttggc ctgctgcagt ccggtgacct
    14701 tgcgggcttc gacgcgatgg tcgccaactt ccaggccacc agccagcgga tcaagtattc
    14761 gctggtgccg gtggtcgcgg cggtgcgcgg gctggcgctg ggcggtggct gcgaattcca
    14821 gatgcacagt gcgcgcgcgg tcgcccacct tgaaagctac atcggcctgg tcgaggccgg
    14881 ggtcggcctg ctgccggccg gtggcggcct gaaggaactg gcggtgcgtg catcaaacgc
    14941 cgccggtccc ggcggcgacg tgttcgccga gctcaagaag gtgttcgaga ccatcgcgat
    15001 gggcaaggtc tcggcctccg cggtcgaagc gaaggaactg ggcctgctgc gcaaggacga
    15061 cctggtgtcg ttccacgcgc acgagctgct gcacgtggcc aaggccaccg cgcgcggcat
    15121 ggccgaggcc ggctatcgcc cgccgctgcc cgcgcgccgc gtgcaggtcg ccggcgacgt
    15181 cggcatcgcc accttcagga tgatgctggt caacatgctg gaaggccgct tcatctcccc
    15241 gcatgactac gagatcgcca cccgcatcgc caccgtgctg tgcggcggcg aggtcgaacg
    15301 cggttcgctg gtggacgaag actggctgct gcggctggag cgcgagcatt tcgtggcact
    15361 ggcgcaaatg ccgaagaccc aggaacgcat cgcccacatg ctgaagaccg gcaagccgct
    15421 gaggaactga acatgaccaa gcaaatccag gacgcctaca tcgtcgccgc cacccgcacc
    15481 ccggtcggca aggccccgcg cggcgtcttc cgcaacaccc gtcccgacga catgctggcg
    15541 cacgtgctgc gcagcgtggt ggcgcaggcg ccggggatcg acgtcaaccg catcgacgac
    15601 gcgatcatcg gctgcgcgat gccggaaggc gagcaaggca tgaacgtggc gcgcatcggc
    15661 gtgctgctgg ccgggctgcc ggacacggtg gccgcgcaga ccatcaaccg cttctgctcc
    15721 tccggcctgc aggcggtcgc actggccgcg gaccagatcc ggctgggcaa cgccgacctg
    15781 atgctggccg gcggcaccga atcgatgtcg atggtgccga tgatgggcaa caaggtcgcg
    15841 ctggcgccgt cggtgttccg cgacgaccac gtggccatcg catacggcat gggcatcacc
    15901 gccgaaaagg tggccgagga atggaaggtc tcgcgcgagg agcaggacgc gttcgcctac
    15961 gcttcgcacc agaaggcgat cgccgcgatc gccgccggcg agttccgcga cgagatttcg
    16021 ccctacgacg tggtcgcccg catgcccgac cttgccggca atgccgtgcg cctgaagcag
    16081 ttgcgggtcg agaacgacga aggcccgcgc cttgatacca ccgtcgaggg cctggccaag
    16141 ctgcgcccgg tgttccgcaa cggccagttc ggcggcagcg tgaccgccgg caacagctcg
    16201 cagatgagcg atggcgccgg tgcggtgctg ctggcctccg agcaggcgat caaggactac
    16261 ggcctgaccc cgctggcgcg cttcgtcagc ttcagcgtgg ccggcgtgcg cccggaagtg
    16321 atgggcatcg gcccgatcgc ggcgatcccg aaggccctca agcaggccgg gttgacccgc
    16381 gaccagctgg actggatcga actcaatgaa gcgttcgccg cgcaggcgct ggcggtgatc
    16441 aaggactgcg ggctcgaccc ggccaaggtc aacccgcttg gtggcgcgat tgccctcggc
    16501 catccgctgg gcgccaccgg cgcggtccgc accgcgacca tcgtccacgg cctgcgccgc
    16561 cgccagcaga agtacggcat ggtgaccatg tgcatcggca ccggcatggg cgcagcgggg
    16621 atcttcgaag ggctgtgacc aagctgcgcc tgcgataggc ctacgtctgc ctggccgggc
    16681 gtttgccgcg aaatcaagga ggaggcatcg gcctttggcc gatgccgggg gacattcgcg
    16741 gcaaacgccc gctcaggcag acggagcaac tgccggaaaa tcaggtctcc acgacgccca
    16801 tttcgtccag cgcgttctgc atcagcttgc gtgcggcatc gctgagcaat tcgtggtcca
    16861 gcgcgtagcg cacggttgcc tcgatcagtc ccaggtgggt gccgcagtcg aaccgcgtgc
    16921 cgtggaagcg gaacgcgtcc acctgctcca gcggcaacag ttgcgcgatg gcatcggtca
    16981 gctggatctc cccgcctgcg ccctgctgcg tggtctccag caggtcgaag atcgccggat
    17041 tgaggatgta gcgcccgacc accgcgaggt tgctgggtgc atcttccggc ttcggcttct
    17101 cgacgatccg ggtgatccgc ccggcctgcc cgtcgaagcc ctcggtggcg acgatgccgt
    17161 agctcttggt ctgctcgggt ggcacgtcct gcaccgcgat cacgctgcca ccggtccgct
    17221 ccgcatggtc cgccatctgc ttcagcgcac cggcgccacc ggccaccgac tgccgggtcc
    17281 agatcaggtc gtccggcagc agcacggcga acggctggtc accgaccacc ggcttcgcgc
    17341 acagcaccgc atggcccagt cccaatgcct cggcctgggt cacgaacacc gccctcacgc
    17401 ccggcggcaa cacgttgcgg atcagctcca gctgctccgc cttgccggcc ttctccagct
    17461 tctgttccag ctcgtaggcc ttgtcgaaat agtcggccac cgcgtgcttg tagcggttgg
    17521 tgatgaacac cagggtgtcg caccccgctt ccaccgcctc gtccaccgcg tactggatca
    17581 gcggcttgtc gacgatcggc aacatctcct tcggcacggt cttggtggcc ggcaggaagc
    17641 gggtgccgag gccggcgacc gggaacaccg cgatccgcac gcggcgatgg ggcttggggc
    17701 tttgcatggt gcgcctgggg tcagttcggg cgcgcgcggc gcgccgggaa attcaggatg
    17761 gtcgtggatg cgcgggcgct gtcggcctcg ctggccgggt tgaattccgg caccgcggcg
    17821 cgcagcgacg ccgccagccg ctcgaggtcg tagctggcgg tggcctcgcg cagggcctgc
    17881 agggccgggt ccacccgctc cacgctgacg ctgcgcggca acgcctgcag gatcttcggg
    17941 tggatcgtcg cgctgtagcg ttcatcgacg tggaacaggg tttcgtgcag cttctcgccg
    18001 ggacgcaggc cggtgtattc gatcgcgatg tccttgcccg gttgcttgcc ggcaagccgg
    18061 atcatctgct cggccagcat ccggatcgac accggctcgc ccatgtcgag ggtgtacacc
    18121 gcctgttggg caccgatcgc cgaggcctgc aggatcagct ggcaggcttc cgggatggtc
    18181 atgaagtagc gggtgacctc cggatcggtg accgtgaccg ggccaccacg gcggatctgg
    18241 tcgcggaaca gcggcaccac gctgccggcg gaatccagca cgttgccgaa acgcacggtg
    18301 acgaaacggg tcgaacgcgg atccaccagc gcctggcaca gcatttccgc caggcgcttg
    18361 gtcgccccca gcacgttcac cgggtccacc gccttgtcgg tggagatcag cacgaaggtg
    18421 ccgaccccgg cgtcgcggct ggcgcgcgcg accacgtcgg tggccagcac gttgttgcgc
    18481 accgcttcgc gcagctgccg ctgcagcagc ggcacctgct tgtaggcggc ggcgtggaac
    18541 accgcttccg gctcggcgat cgccaacgca tgcgcgatca ccgccgggtc gccggcatcg
    18601 ccgagcaccg gcaggcattc gatcccgggg aagtcgcggc gcaggtcggc ttcgaccgtc
    18661 aacagcgcca gttcgtcgat ctcgagcagg gcgatccgct tggcgccgtg gcgcgcgcac
    18721 tgccggcaca gttccgaacc gatcgaaccg ccggcgccgg tgacaagcac cgaacgcgcg
    18781 cccagccagt tgcggatcgc cttccagtcc gggctgagcg gctgccggcc gagcaggtcc
    18841 tcgatcgcca cttccttgag ctggccgggc agcgagcggc cgtccagcac gtccgacagc
    18901 cgcggcacgg tgcggaacgg caccccgctc tcctcgcaga tgccgaccac gcgccgcatc
    18961 gcgatcgcat ccagcgacgg catcgcgatc accagcagct gcaccccggt ctcgcgcgcg
    19021 atgcgggcga cgtcgccgac ccggccgagg atcggcaggc cctgcaggcg ggtgccgcgc
    19081 agggccgcgg cgtcatcgac gaaaccgacg ggctggtagc gcccgatccg gcgcaggtcg
    19141 cgcaccaccg cttcgccagc ctgccccgcg cccagcacca gcacccgctc ggcggtctcg
    19201 tcggaacgca gcaggcgctg gtccttccat gcgcggaaga tcagccgtgg tgcgcccagc
    19261 agcatggtca gcacgaacgg atacagcagc agggccgcgc gcgacaccag gtcgagccgg
    19321 ctgtagaaga acagcccggc gaccaccgcc agcaggccca acacgctggc cttgaagatg
    19381 ttgagcaggt cggggacgct ggcgaatcgc cacagcccgc ggtacaggcc gacccgccac
    19441 gacaccaggc cctgcgccac catcacgatc aggaccgtgc tggacagcgg ttccagcggc
    19501 agcggcgtcg gctgcagggc ataacggatg tagtgcaggc cctgccagca gacccagacc
    19561 atggcgaggt cgtgcaacat caccgccagc cgcggcaagc cgtacttgaa ccggtcccgc
    19621 caactcgtca tgtcccgaat ccttcggtgt tgttcttctg gtaccgccgg tgcagccagc
    19681 gccaggccca ggcagacgcc ggcaaggtcg ccagcgccac caccagcgcg atccgcgcgg
    19741 gccaatgcag cgcggcaagc atgagcgcaa ccgccgcaaa cgtccagacc gcataggcgg
    19801 cgcataccca ggcgtgtccg cgatgccggc tccaacgctg gaaggcatgc tggacgtgcg
    19861 cctgccacca gcgctcgcgg cgccggatgc gccacagcag ggtcaggccg gtatcggcca
    19921 gcatcgccag cggcggcagc agcaacagcg gccaccaggc catcggccgc gccgcgaacc
    19981 cggcggccag cagggccgcg accaggtaac cgagcccgcc gctgccgacg tcgcccagga
    20041 acacccgcgc ccgcgggaaa ttgaacggga ggaacccgag gcaggcagcg gccaatacca
    20101 gcgccagcag ccgccagcca tcgcccagca ccaccgcaaa cccggtcgcg cacaacagcg
    20161 cctggctcgc cgccagcccg ttgatcccgt ccatgaagtt ccaggcattg accagcacca
    20221 gggccagcag gaatgcggcg atggtcgtgg ccggcgcggc cccctcgagg tgcagcgccc
    20281 acgccaggca ggcggcgccg gcagcatgcg ccagcaggcg cggccaggcc ggcagcgggc
    20341 ggtggtcgtc ccaccagccg atcgcggcga ccagcaccaa gccgcaggca cgccacagcc
    20401 aggcggacgc gtccggctgc gtccccgcca gtgcgagcaa cgccgccagg cacgccatca
    20461 cgatcccgat gccgcccccg cgcggcgtcg gcagcgcatg gctgcggcgt tcgccgggct
    20521 ggtcgaccag gccttcgcgg cgggcgtgga ggattgccca gcggctgccg agcatcgaca
    20581 ccagcagcgc ggccaacgcc gccgccagca gcaccaccgg cgtcgggatc gccggctcag
    20641 accacggcat agttcagcag cggcttcacc gttccccact ccttgcagct gggacattgc
    20701 cagtggtggc tgcgcgcgcc gaaaccgcag cggttgcagc gatagctggg gttgcgcacc
    20761 agcaactggt cggtgatgtg cttgaggtcg tgcagggtcg cctcggcatc cgcgttctcg
    20821 gcaagcgtga ggtcgatcaa cgccgcttcg ccgcgtaccg acggccggtc cttcagctgc
    20881 tggccgaggt agcgccgggc cgcacccacg ccctcttctg cctcgaccat cttggtcagc
    20941 gccagcaccg gcgagatccc ccggtagtgc tcgctcatct cggcgaggaa cgcgcgcgcg
    21001 ccggtgcgtt cgccgacttt cccgtagctt tccagcagcg ccggcaggat caccggcagg
    21061 tagtcgggat cgtggcgggc ggcgcgctcg aacgcacgca ccgcgccggc gggattgcca
    21121 gcctcggctt cgatccggcc ctcgctgatg ccggcgcgca ccgaggtcga atcggccgcg
    21181 taggcacgcg cgatcgccgc acgcgcggca tcgatctcgc ctgccgagcg ctggcggtcc
    21241 gccagttcgc attcgaactg ggcgaccagc ttgcccattg gctcggcggt cgcggcctcg
    21301 tagcggctgg cgttctcgat cgccttctcc cagtcgcgtt cggcctggta gatgctgatg
    21361 aggtggcgca gcgcctgcgg cgcgcgctgg tcgatccggg caaggtcgct gaacacggtt
    21421 tcggcgcgat ccagcaagcc cgagcgcatg tagtcctcgc ccagcgcaag cagtgcctgc
    21481 accttctggg cgtcgttcag gtcggggcgc tgcaccagcg cctggtgcag gcggatcgca
    21541 cggtccacct cgccgcggcg gcgaaacagg tgacccagcg ccacctgggt ctcgaaggtg
    21601 tccttgtcga gctcggcgat gtgcaggaac agctcgatcg ccttgtcggg ctgctcgttc
    21661 aacaggtagt tgaggccgcg gaaataggtg gtcgagagct tgctgacctg gctgtcgcga
    21721 tggcgttcgc caccgcggcg accggtcacc cagcccgcca gcgccgccag cggcaccagc
    21781 accacgaacc agatccagac gtcgaaattg cccatgtcag gtctccgcgc gcgcgctgcc
    21841 ggcggcaacc gggcgggcct gctcggcacg ccgcaggcgc cggcgcagcg gcgcgaccac
    21901 cccgatcgcg aggatgccac cgccaaccac cacgcccagc agcaacgcga ccagcacgct
    21961 cagccccagg ctggcgcgca cgctggcgaa gccgatatcg agcagcacgg gctgggtgtt
    22021 gagtgcgccc acgatggcgc ccagcaggat gcaggtgacg gcgatgatga ggcggatcag
    22081 gcgcatcggg aactccggga tcgcctgcta gcttaacgga cggccgcgga tcagtcgtcc
    22141 atcggcagcg gcacgaccgt ggtcacccgc tcgcgcaatt ccttgcccgg cttgaaatgg
    22201 ggaacatgct tgccgggcaa cgcgacggcg tcgccggtct tgggattgcg gccggtgcgt
    22261 ggcggccgga aatgcaggga gaagctgccg aacccgcgca cctcgatacg gtcgccgccg
    22321 gcgagggccg cgcccatcat ctgcagcagc gacttcaccg acaggtcgac gtcgtcggcc
    22381 ttcaggtgcg gctggcgccg ggccagcaat tcgatgagtt cggacttggt catgaaccgt
    22441 ggggcgggga atgcggccgc atgaaaatgg cgacccggcc gaagccgggc cgccacgtgc
    22501 tgctaggccg ggtggatcac tcggacttgc tgttcagctg ctcgcgcagc agcgcgccca
    22561 gcttggtggt gccgctggcg gcgtcggcgg aggccttctg gtactcggcc atcgcttcgg
    22621 cggcctcggc atcgtccttg cccttgatcg acagctgcag gctgcggccc ttgcggtcca
    22681 tgcccacgaa cttggcctcg acctgctggc ccaccttgag gtagtggctg gcgtcctcga
    22741 cgcgctcgtc ggcgacgtcg cgcgcgaaca cgtagccttc cacgccatcg cccaggtcga
    22801 tggtcgcgcc cttggcgtcc acttccttga cggtgccggt gaccttggtg cccttcgggt
    22861 tggtcgccat gaactggccg agcggatcct gctccatctg cttgacgccg aggctgatgc
    22921 gctcgcgctc cggatccacc gccaggacga cggcttccag ggtgtcgccc ttcttgtagt
    22981 tgcgggcgat gtcttcgccg gtggagttcc agctgatgtc cgacaggtgg atcaggccat
    23041 cgatgccgcc gtccaggccg atgaagatgc cgaagtcggt gatcgacttg atctggccgg
    23101 acaccttgtc gttcttcttg tggatcgcgg cgaaggtctc ccacggattg ctggtgacct
    23161 gcttgatgcc cagcgagatg cggcgacgct cttcatccac gtccagcacc atgacctgca
    23221 cttcgtcgcc gacctgcacg accttggccg ggttgacgtt cttgttggtc cagtccatct
    23281 cggacacgtg gaccaggcct tccacgcccg gctcgatctc gacgaacgcg ccgtaatcgg
    23341 tgacgttgct gaccttgccg aacacgcggc tgttggccgg gtagcggcgg gcgatgttgt
    23401 cccacggatc ctcgcccagc tgcttcaggc ccagcgaaac gcggttgcgc tccttgtcgt
    23461 acttgagcac gcgcacgtcg agctcggcgc ccacttccac cacttcggac ggatggcgca
    23521 cgcgcttcca ggccatgtcg gtgatgtgca gcaggccgtc gatgccgccg aggtccacga
    23581 acgcgccgta atcggtcagg ttcttgacca cgcccttcag caccgcgccc tcgaccagct
    23641 tctcgagcag ctgctcgcgc tcttccgaat gctcgctctc gaccaccgcg cggcgggaga
    23701 cgaccacgtt gttgcgcttg cggtccagct tgatcagctt gaactcgagt tccttgcctt
    23761 ccaggtacac cgggtcgcgc accgggcgca cgtcgaccag cgaacccggc aggaacgcac
    23821 ggacgtcctt gatgtcgacg gtgaaaccgc ccttcacctt gccgctgatg cggccgacga
    23881 tggtggcgtt ggcttccagc gcctcttcca g
//
LOCUS       DAORUK010000015        22942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_1823_length_22942_cov_17.994626, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000015 DAORUK010000000
VERSION     DAORUK010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 22942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22942
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1823_length_22942_cov_17.994626"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PK818_01980"
     CDS             <1..797
                     /locus_tag="PK818_01980"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HQY81535.1"
                     /translation="LGETVGGQILNVNADFAANELVQVLQPYKIVFLTGTGGLLDADG
                     RVIDSINLSTEYDHLMQQPWIDGGMRVKIEQIKDLLDGLPLASSVSITKPAELAKELF
                     THKGSGTLVRRGERVLQADHWGAFDLPRLRGLIESAFGRRLLPDYFETTKLHRAYVSE
                     NYRAAVILTSEAAGIYLDKFAVLDEAQGEGLGRAVWQVMRDANPRLFWRSRRGNPVNP
                     FYYAESDGCIKQEKWKVYWYGPAGFDLLERYVETCAARPASLEEPA"
     gene            794..1768
                     /gene="argC"
                     /locus_tag="PK818_01985"
     CDS             794..1768
                     /gene="argC"
                     /locus_tag="PK818_01985"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HQY81536.1"
                     /translation="MSGLRVGLVGARGHVGGELIRLLAGHPRFELAYVTSREREGQPL
                     AAHIAGYAGDLHYSSPAYEELPELGADAVVLALPNGKAGHIVQQFDDAAADVVLLDLS
                     ADFRFDPSWYYGLPELTRAQYVGQRRISNPGCYATAMQLAIAPMANALEGPVQCFGVS
                     GWSGAGTTPCDRNNIELLHDNLMPYALAGHVHEREVARQLGHPIEFMPHVAAHFRGLT
                     ITANMHLAIPFDLEEVLARYHHRYAGEPLVRVQDAAPWVSRIAGAHHVDLGGFTLGGD
                     GKRLVVVATEDNLLKGAATQALQNLNLAFGFDELAGIPLPADGEEVRA"
     gene            1765..3060
                     /locus_tag="PK818_01990"
     CDS             1765..3060
                     /locus_tag="PK818_01990"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HQY81537.1"
                     /translation="MSNLLWQKPGVAVDAGIQAFLAGDDVLLDRDFFLHDIAASAAHA
                     EGLQRIGILSADELDGLKRELATLADDFRSGGFVLDARFEDGHSAIEARLTERLGDAG
                     RKIHTGRSRNDQVLVATRLWLKDRLAQLALLSREIARVALDRAGAERDLPIPGYTHIQ
                     RAVVSSAGMWWAGWAEAFIDDAVRAADTLALVDCNPLGTAAGYGVNLALDREHTTEAL
                     GFARMQVSPVYAQLSRGKFELAALEALGSATLDLRRLAWDLSLFSSAEFGFVALPAQY
                     TTGSSIMPNKRNPDVIELMRATHASVAAARTEIEQLLSLPSGYQRDLQASKGAIVHGF
                     GRGLAALALLPDLLANIGWREDRLRAAIDAGMYATDVAVEAAIAGVPFREAYKAAAVS
                     GDSAGQGRTPEGSLAARTSPGAAADLRLDALHARLAALG"
     gene            3064..4206
                     /gene="proB"
                     /locus_tag="PK818_01995"
     CDS             3064..4206
                     /gene="proB"
                     /locus_tag="PK818_01995"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016944476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HQY81538.1"
                     /translation="MAGFTPQSLPHWRRAVLKVGSSLLADAGGGLADVHAAGLARFIA
                     QSREQGREVVLVSSGAVAAGRGRINAGGDGIAMKQALAALGQAALIGFWQALSAAPVA
                     QVLLSHDDLRNRRRYLNARAALQELLRLGALPVVNENDTVAVDELKLGDNDNLAAAVA
                     SLVDADLLLIATDIGGLYSAHPQRDPDARPIAQVDQVTPELLQAAGGGAGALGTGGMR
                     TKLEAAGKAASAGIPSVLFCGRDPAVVAALGAGVLHGTLVAALANRRSARKQWLGHAP
                     ASGVLRIDAGAGAALLRGASLLPGGVLAVAGDFRRGDVVEVRSEDGIVLARGQAQYNA
                     ADGARIAGRHSREIETILGFRYGEALVHRDDLVLLQHPEDGKEAMA"
     gene            4203..5468
                     /locus_tag="PK818_02000"
     CDS             4203..5468
                     /locus_tag="PK818_02000"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HQY81539.1"
                     /translation="MSGYVRQLALAARAAQAAVAACDAATRRALIVAMAAQLEARLDV
                     VLRANADDLRRAATAGAGKAVLDRLLLDAGRVRGMAAALREVAAQPDPLGAITRSERR
                     PNGIMVERQRIPLGLVAMIYEARPNVTAEAAALCLKAGNAVLLRGGSEAAASNAAIAA
                     CLHAALRDVGMPEAAVSLVADTAREHVLELLQLTDLVDLAIPRGGESLIRFVAEHARV
                     PVIKHYKGVCHLYVDRAADIGTAIGLLIDGKVSRPGVCNALETLLVHRDIAEEFLPPA
                     LAALRAHAVEVRGDARTRAIDAGVLPATEADYAAEFLDLVIAVRVVDSLDQAIAHIRQ
                     YGSDHTEVIATADPVAADAFVRAIRSSAVMVNASSRFNDGGQLGLGAEIGISTTRLHA
                     YGPMGAESLTIERFVVRGEGQLRQPPAAT"
     gene            5678..7915
                     /locus_tag="PK818_02005"
     CDS             5678..7915
                     /locus_tag="PK818_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HQY81540.1"
                     /translation="MPRVAPTKLRFVPFLLASILLSTLLPVSPVRAACAPDPGSGLQE
                     SPPAASAQEAAPASDLPAPQAGDADADSEARAALAAISRLDDADTALALAADGAALYD
                     REAVKLDGYAYCSQAVELAEKGEFRESARAASKALHVALQTGNKDLLGKAYRDLAIAF
                     NYAGQLERAEQFANLALKYPGVNPTQVNGPARKVIGDVRTRQGRYDEAIASYEEARQG
                     SSDRFRPLVEASLANALILSGDLVRARSTLDALAEPQEAKQRAQLQRTRGRLLLAENK
                     PAEALALYQALASAPADGDEDFYRAWALDGMSKSQAALGNDAEAAQSLDQAIDVFDQA
                     RSQFRSDEFKMGLFSDLQNVFERAIGMHSKLGQPARAFDISERSRSRALLDAVAGRAE
                     LKGDGGKALDAASLQKMLRADEVVVAYHALPDRLMAWVLSSEGVREAAFPAALPRNDL
                     ARLVDAYRDALIKLNPNAAAIGDKIGQLLLAPLGVEAGKRVVIVPHGPLHYLPFQALR
                     LDGQYLIERNPISIAPSISIAARLAERTPSVEAQLLAFGNPTIDPNVADPLPGAEREV
                     HELSRQFPGAKLFFKDEANKANFTANAPQSRLVHIAAHATVDTLDPLHSKVLLADENG
                     KPNYLEARDVIGMDLGNVAMIALSACESGLGRVENGDEVLGFTRSFLSAGTSTLLASL
                     WPVSDAATEKLMTTLYAELAGGEQVQDAMRDAQRAVMADPETTHPFYWAPFNLIGNWR
                     LKVAK"
     gene            7957..9606
                     /locus_tag="PK818_02010"
     CDS             7957..9606
                     /locus_tag="PK818_02010"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HQY81541.1"
                     /translation="MPPTNLLAAALLMACVPFAQAADGAAAQEATPAPGSAPQQAQAL
                     PEPQMAVQAQASFVLRGVQFVGATGVADSELQAAVADRIGTSVTFADLEQLAARAVAV
                     YQKRGFGLVQAYVPVQEVVDGVVRISISEGVLGNVSIELAEGVPVAQERVAGTLAILE
                     PGKPLNGRQYERAMLLLSDLPGIKPQSAISAGAATGTTDLVVQVGERDRLQYGLEVDN
                     HGTSDSGRHRITGSLRWASPFGRGDNLDLRLMLAEGMHTAFGRISYETPVGYRGLRIG
                     GGLARVQYELGGPFAPLEPTGTGNVADLSFSYPLIRQRSTNLFVRGVIDNKDLTDRFE
                     AVGFETHKRVRGAGIGLSFERRDGLLGGGYTSANALLYRGNLDIKDATSEAFDNPPFG
                     YDTEGSFGKATLQLARLQSLAPKFSLFLGTGMQRASKNLDAYEKLSLGGPKAVRAYAT
                     GEVLVDDGWLATVELRYAPRPDTTLFAFYDAAHGDFFHDPRPFDVFTSRSLRGHGLGL
                     NWSRPGKLSMNFSIAWRSTDPGVTDGGDRNPRLFWSIQKAF"
     gene            9617..14845
                     /locus_tag="PK818_02015"
     CDS             9617..14845
                     /locus_tag="PK818_02015"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HQY81542.1"
                     /translation="MRTKLTSKQLRCLRRRRLALALVGAMALPAAVAQNLPTGGNVVN
                     GTASINEAGNQMTITQTTRGAIINWGTFNIANGFGVAFNQPGNGVTLNRVLGTMPTAI
                     NGILTANGNVFIINTNGITFGANAQVNVGGLVASTLALSDGDFLTGLSTGRYLFAGNT
                     GTPVLSNAADITAGAGGVGFVGTNLRNSGAIVANGGNIGFGAGTSVTLDFVGDGLTQV
                     TINAAPAVDTQLFQDRFGSMTADGGRILLRNAATAAGTGGVIYADGLLRAQSIANVNG
                     RIELTSIGGPVFLGAPGVLSDASTPFTAGRIDVSGDVGETGGSVLIQGNGVAFLNNDP
                     TPLAPADPLSVGSFINASGGEGGGSVTIQSTADVTMLGLSGISADTQRGVAGQVLVQA
                     VGSIGMAAATVISADAMDAGDGGQVGLAAGGNVVVAGLLSAKGAKGGGNGGTVAMSSQ
                     GGAAVLQGGIDVQASQGNGGDVLIVGETASGLANGASVNASGGIDGGSVVVSANNGGA
                     VLGETASIDARGLGGNGGQVALAGTSVYAYGAIDAHGAVGGGLVGTIARGGDLDLRGL
                     DVDAGGGSTAGMWMIGGASLEVVNGNDQGSLTATQAGSSVQDADINAALDTGTSIQLV
                     TSDGGVNFDDARISTSSPLALTFTVNSESSITGGTFDIESTGGSLAMDFNADADGDDP
                     GEGFIAFLGANLSSNGGDIRFFGGSDPLAGAATGYGSGIRLNRVAIDTAGGDVLLRGA
                     STGADTGPDDAGVALEFSSIDTDGGDVVVEGTGAGDTGGVFLWSGRIDASGGGGTGAI
                     TITGASAAAFNAVDLDGFGLLADAGAISLQGDAGADGTGVALRASLLDSNGGAISVVG
                     SSVAGVGLSFAEGSALASAGGAVLLQGDGALAGVELLDGGIDGGGGDITVIGSASAAN
                     AIGVYSDGVDLVGGAGDVTVDGASPLGIGVLFANGAGVSTTSGAITLIGTGANFGLDI
                     ADGAIDTDSGDITLSGEALAATATAGVRVTGGGLATNGGSISVSGTSAGGVGVQLGDG
                     GAFAIGSGGGAISINGSGVTAGVLMQGNTVASGGGVIDVIGAASGGNGTGVDLANAQL
                     VAGAGDVRVFGNAAAGTGVNFAGQSGISTTTGAIAVTGIGATVGLALAGGELSTESGH
                     FDLRGRGTGAGSDGLVIGQGVSIATNGGGIELSGEGGSGAGVSLEAGSSVDAGNSLVV
                     VRAGNDGSSDALRIAGTIASGMAVNLRPGGVDANGGLTERTADEILIGGGSAGFALSG
                     AELALIDAPELIIGSNLHAGAIRVLGATSRSGDLTLQNDGGSGGIDIQAALDVGTGTL
                     ALSSGGDITQSSGGAITAHSLLARAGGSVLLADAGNNVAATTLAGSAGGDFEYEDVDA
                     LAIGSVSGLGMDASSNGLASFGATGISAGGDVFVRNLAGDMTLNAGVSGVNIDLVTAN
                     TLQNAAGASLVASGDWRVWANSWVGEDRGGLAGDGNLPNLYGCAYLGQCGVTVPAADN
                     HFIYVQQPTAVITFDDFMREYGLPNPTFTFTVSGAILGDSAANVASGVGVTTATIGSN
                     VGNYPITGQFTSAAGYRLQFVPGTLGITPATLLFTADSFIRYLGTENPLFTGSVTGFR
                     NGDTIESVFGSGVIWNSPAGLLSPIGFYPVNGGSSATNYVFVQAPGNATALQIIPLPQ
                     LSSTPIDLIRETVNTYVYDRNFGGAPMCALNASLEDQQLASSGDELSNEWAKVRSRPN
                     LTNCFETERKNSCGDF"
     gene            complement(14870..16234)
                     /locus_tag="PK818_02020"
     CDS             complement(14870..16234)
                     /locus_tag="PK818_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY81543.1"
                     /translation="MEAIQQFALASGIAIGGGLLGAAVSAILLRRAQARQAHRLAAEV
                     AAGNLPPAVAAPSLAPLLDALSQRIALMQQARDELDAQRRQLSADMVRQMQQTDGAMR
                     AKDRFLSAISHDLRQPLQTMDLALAQLRREPLPAQARELVQLDAGMRTLAEVLDGLAL
                     LSQLDAGALQAHASACELEQAFASALAAQADAARRAGVSLHAHAGGHAVFTDPRLLGS
                     LLERLLDNAIKASPGGARVLLAARRHGTQVRLEVRDGGVGIAPIHQPRVFDEFFQVGN
                     PERDHRKGFGLGLPIVSRLAALLGTRVELRSRLHAGTCFWLALPRASVLQRPPRAVLL
                     DRDPGHRDALAAMLRSWGYAVQAESAVDAACTHLAGGNGGLDAVLCAIDADDDPAWGL
                     LRAAAAQQAQAARIVLCASPGPGLLELAARHGAQALSRPPAPAKLRSLLAQRPLPQSM
                     RGAA"
     gene            16481..17053
                     /locus_tag="PK818_02025"
     CDS             16481..17053
                     /locus_tag="PK818_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQY81544.1"
                     /translation="MSTTTILRSALAIALLAACAGLHAQDAQDPQSAPSVDSIIESLK
                     ADAPAQQPGQTRALRPGAASMSTAPVAAPAKPKPASINMQINFEYNSAEIAGSSEKTM
                     ATLAKALASPQLEGRKFTVIGHTDASGSAAYNKALSDRRAAAVRRYLTENGVSAARLK
                     AMGKGESQLLNTEDPNAGENRRVEIQATGG"
     gene            17064..17453
                     /locus_tag="PK818_02030"
     CDS             17064..17453
                     /locus_tag="PK818_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4280 domain-containing protein"
                     /protein_id="HQY81545.1"
                     /translation="MPMQVVNGAVLMCSFGMAPSNLVVLPVNRVLSGNQPAANIMDHK
                     PMVNILPFGMCITPSNPQVAAATAAAQGVLTPQPCIPMTQTPWVPGAPTVLLANMPAV
                     DNTCTCMCLWGGVVSVTSPGQFTEMIP"
     gene            complement(17517..19073)
                     /locus_tag="PK818_02035"
     CDS             complement(17517..19073)
                     /locus_tag="PK818_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81546.1"
                     /translation="MFEGLTEGLSNIGAAAGEAVQQAQQQAAAAAGAAANAAQGAISE
                     AQTAVANASAAAAGAANGAVAQAQQAAQQAAAQAGAAAQGALGQANQAMDAARSAAND
                     MAQNALAQANNALTDASQQAGAAAQNALAQAQSAVNTAQNQAKAIADGALAAATGALE
                     NAMATAEQAAQSAIDNAMGMVDNAMGMLEDGVGNALAAANNAIDQAQEQLRDAATNAL
                     QQARSAVIDNARGALGQVAAALDALPTSFPNLPVDFPASDCPYVNRLRDELARRAAQA
                     NNALGNAVGALNDIAGPMLDQASNALDQANGLADQALGAMDDAMGALDQAQATANGAA
                     QQALGAARTAVQQAQQAVREQAGAAIAQVKDAVNGMKQQAQDAAQGAIGDAMAAVDSA
                     QQQAGDAAGQAIAGAQAAAAQAQQQVNQAAQQGLAQANQAAQQAQQALSGAAQGALNN
                     AQNAAQAAATQAGDAAQGAIASAQSSIGQASAAAGQAAQGAVGAAQSAVAGAAGAMGN
                     AAQSLGNLFG"
     gene            complement(19075..19431)
                     /locus_tag="PK818_02040"
     CDS             complement(19075..19431)
                     /locus_tag="PK818_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81547.1"
                     /translation="MLAVKRKQMAAIGEAQLRNNLADFLGRHVDGLSSLPLDRLDAEL
                     DAIIAYCRKAGLRSQRAVASYALACSLFGNDRVAGDPSIIGVLADRSSSQLDRALLIE
                     MWTAAAYGDYRRTQGG"
     gene            complement(19445..20014)
                     /locus_tag="PK818_02045"
     CDS             complement(19445..20014)
                     /locus_tag="PK818_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HQY81548.1"
                     /translation="MNARRRDQVIPQGQYESLHKLVFGQRARAVYAVMDGAMIEGLPG
                     RLAEIAPDAACLFEGALDPMLSAAAPWLVKLDPDSTATQMALRDGWNGHWGIVLQTDP
                     ALDLRAVRSHLRRVLRVRAPDGASMLFRFYDPRAFRTVIPVLDPPARREFFGPIHGVF
                     VEGRSPDSVLFFARDGRPEPQALPLASAA"
     gene            complement(20142..22268)
                     /gene="tssI"
                     /locus_tag="PK818_02050"
     CDS             complement(20142..22268)
                     /gene="tssI"
                     /locus_tag="PK818_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HQY81549.1"
                     /translation="MPSSLSTSNRMYFVTLHDYGEDDLLIEEMTGQEAMSQLFQFRLR
                     LLSERDDIDPRRIIAKPAILRTETRDIHHMGGERHWNGVVSRFQRTGRTASPDGDSVY
                     SYECDIVPWFWFLTQHEDCRIFQNKTVREIVEAIFEEFAYTDFRFDLTENHPSLEYCT
                     QYHETTFDFISRLLEREGIHYYFRHYEEGGETRHLLVFSDNNGSNPALDPDTVPFDEE
                     GHTEEFDAIQALALDQQMRTRKVTLGDWDFVRKRLLREDTPTVLSIGADRDLERYRYP
                     GGFVEPALGKYRSRVIMEAEESSHMRFRGNGKVRTLAPGHTFTLEDHPFDEFNIEYMV
                     LSVWHHGRNNLTGNGAPSDYANEFSLQPHEVTWRAPLSTPKGLVRGPQTALVTGPAGE
                     EIHTDQYGRIKIQFHWDRLGRYDDRSSCWVRVAQTWAGNGFGTMFIPRVGMEVVVDFL
                     EGDPDQPIVTGCVYNGVNRPPYAPPGPGPATKSGIKTLSSKGGGGTNELLFEDKKGSE
                     EVFLQAEKDLNVKVKANRTESVGGDSNHDLAGKQRIYVGGDRHLKVDGASTMVVKADL
                     MQGSKGDQELLSEQELRLLGKSSVRIGSRGGIDIHGGMKLVMEANTLISLKVGGSGIV
                     IDPSGVSVIGPIIKQNSGGAPSAASAPSGFSAEKAQGSHGTEDGQVTDPGQQLQAQAL
                     RNAALAGQPFCAECEAARAAYAALMA"
     gene            complement(22392..>22942)
                     /locus_tag="PK818_02055"
     CDS             complement(22392..>22942)
                     /locus_tag="PK818_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY81550.1"
                     /translation="PDVLELFFQVFDKGRMEDGEGREIDFRNTLIILTSNAGTDLVMQ
                     ACNESEELPTVAYLNELLKPELNRTFKPAFLGRTAVIPYYPLRDENMRKIVRLKLNKI
                     GKRIKANHGATLEYDEAVVDAIARRCTEVDSGARNIDNILTGTVLPEVSGHVLERMAE
                     GGTISEIRIAVGGDGGFAYAVT"
BASE COUNT         3388 a         7920 c         8185 g         3449 t
ORIGIN      
        1 cgctcggcga gaccgtcggc gggcagatcc tcaacgtcaa tgcggatttc gccgcgaacg
       61 aactggtgca ggtgctgcag ccgtacaaga tcgtgttcct gaccggtacc ggcggtttgc
      121 ttgatgcgga cggccgggtg atcgattcga tcaacctgtc caccgagtac gaccacctga
      181 tgcagcagcc gtggatcgat ggcggcatgc gggtgaagat cgagcagatc aaggacctgc
      241 tggatggcct gccgctggcc tcgtcggtgt cgatcaccaa gccggcggag ctggccaagg
      301 agctgttcac ccacaagggc tccggcaccc tggtgcggcg cggcgagcgc gtgctgcagg
      361 ccgaccactg gggcgccttc gacctgccgc gcctgcgcgg gctgatcgag tccgcgttcg
      421 gccgccgcct gttgccggac tatttcgaga cgacgaagct gcatcgcgcc tacgtcagcg
      481 agaactaccg cgcagcggtg atcctgacct cggaagcggc cgggatctac ctcgacaagt
      541 tcgcggtgct cgacgaggcg cagggcgagg gcctggggcg cgcggtctgg caggtgatgc
      601 gcgatgccaa cccgcggctg ttctggcgtt cgcggcgcgg caacccggtc aatccgttct
      661 actacgcgga atccgacggc tgcatcaagc aggagaagtg gaaggtgtac tggtacggtc
      721 ccgccggttt cgacctgctc gaacgctacg tggaaacctg cgcggcgcgc ccggcatcgc
      781 tggaggagcc ggcatgagcg ggttgcgcgt cggcctggtg ggtgcgcgcg gccatgtcgg
      841 cggcgaactg atccgcctgc tcgcggggca tccgcggttc gagctggcct acgtgacctc
      901 gcgcgaacgc gaaggccagc cgctggccgc gcacatcgcg ggctatgccg gcgacctgca
      961 ttacagctcg cccgcctacg aggaactgcc ggagctcggg gcggatgcgg tggtgctggc
     1021 gttgccgaac ggcaaggccg ggcacatcgt ccagcagttc gacgatgccg ccgccgacgt
     1081 ggtgctgctc gacctgtccg cggacttccg cttcgatccg tcctggtatt acggcctgcc
     1141 ggaactgacc cgcgcgcaat acgtcggcca gcgccggatc agcaatcccg gctgctatgc
     1201 caccgcgatg cagctggcga tcgcgccgat ggcgaatgcg ctggaaggtc cggtgcagtg
     1261 cttcggcgtg tccggctggt ccggcgcggg caccactccc tgcgaccgca acaacatcga
     1321 gctgctgcac gacaacctga tgccgtatgc actggccggc cacgtccacg agcgcgaagt
     1381 ggcgaggcag ctgggccatc ccatcgagtt catgccgcac gtggccgcgc atttccgcgg
     1441 gctgaccatc accgccaaca tgcacctggc gatcccgttc gacctcgagg aggtgctggc
     1501 gcgttaccac caccgctatg cgggcgagcc gctggtgcgg gtgcaggacg cggcgccgtg
     1561 ggtcagccgg attgcaggag cccatcacgt cgacctcggc ggcttcaccc tgggcggcga
     1621 tggcaagcgc ctggtggtgg ttgcaaccga ggacaacctg ctcaagggcg cggccacgca
     1681 ggccctgcag aacctcaacc tggcgttcgg tttcgacgaa ctggcgggca tcccgttgcc
     1741 cgcggacggc gaggaggtgc gcgcatgagc aacctgctgt ggcagaaacc cggcgtggcg
     1801 gtggatgccg gcatccaggc cttcctggcc ggcgacgatg tgttgctgga tcgggacttc
     1861 ttcctgcacg acatcgccgc cagcgccgcg catgcggaag gcctgcagcg catcggcatc
     1921 ctgagcgccg atgagctgga tggcctgaag cgcgaactgg ccacgctggc ggacgatttt
     1981 cgcagcggtg ggttcgtcct cgatgcgcgc ttcgaggacg gccattccgc catcgaggcg
     2041 cggctgaccg agcggttggg cgatgccggc cgaaagatcc acaccggccg cagccgcaac
     2101 gaccaggtgc tggtggcgac gcgtctgtgg ctgaaggaca ggctggcgca gctggcgctg
     2161 ctgtcgcgcg agatcgccag ggtcgcgctg gaccgcgccg gggcggagcg ggacctgccg
     2221 atccccggct atacccacat ccagcgcgcg gtggtgtcgt cggcgggcat gtggtgggcc
     2281 ggctgggcgg aagccttcat cgacgatgcg gtgcgcgccg ccgacaccct ggcgctggtg
     2341 gactgcaacc cgctgggcac cgccgccggc tacggcgtca accttgcgct ggatcgcgaa
     2401 cacaccaccg aagcgctcgg cttcgcgcgc atgcaggtgt cgccggtgta cgcccagctc
     2461 tcacgcggca agttcgagtt ggccgcgctg gaggcgctgg gcagcgcaac gctggacctg
     2521 cgccggctgg cctgggacct gtcgctgttc tccagcgccg aattcggctt cgtggcgctg
     2581 ccggcgcagt acaccaccgg cagttcgatc atgcccaaca agcgcaaccc cgacgtgatc
     2641 gagctgatgc gcgccaccca cgccagcgtg gccgccgcgc gcaccgagat cgagcaattg
     2701 ctgtcgctgc cgtccggcta ccagcgcgac ctgcaggcca gcaagggggc gatcgtgcat
     2761 gggttcggcc gtggcctggc cgcgctggcg ctgctgccgg acctgctggc caacatcggc
     2821 tggcgcgagg accggttgcg cgcggcgatc gacgcgggca tgtacgccac cgacgtggcg
     2881 gtggaagcgg cgatcgccgg ggtgccgttc cgcgaggctt acaaggcggc ggcggtgtcg
     2941 ggcgacagcg ccgggcaggg gcgcacgccg gaaggttcgc tggccgcgcg cacctcgccc
     3001 ggtgccgccg cggacctgcg cctggatgca ttgcacgcgc gcctggcggc gctgggctga
     3061 gccatggccg ggttcacgcc gcaatcgttg ccgcactggc gtcgcgcggt gctcaaggtc
     3121 ggcagcagcc tgctggcgga cgcgggcggc ggcctggccg acgtccacgc cgcgggcctg
     3181 gcgcgcttca tcgcgcagtc gcgcgaacag gggcgcgagg tggtgctggt gtcgtcgggc
     3241 gcggtcgccg ccgggcgcgg gcggatcaac gccggtggcg acggcatcgc gatgaagcag
     3301 gcactggcgg cgctggggca ggccgcgctg atcggcttct ggcaggcgct gtcggcggca
     3361 ccggtggcgc aggtgctgct cagccacgac gacctgcgca accgccgccg ctacctcaat
     3421 gcgcgcgccg ccctgcagga actgctgcgg cttggcgcgc tgccggtggt caacgagaac
     3481 gacaccgtcg ccgtcgacga actcaagctg ggcgacaacg acaacctcgc cgccgcggtc
     3541 gcctcgctgg tcgatgccga cctgctgctg atcgccaccg acatcggcgg gctgtacagc
     3601 gcgcacccgc agcgcgaccc ggatgcgcgg ccgatcgcgc aggtcgacca ggtcaccccg
     3661 gagctgctgc aggccgccgg cggcggcgcc ggcgcgctcg gcaccggcgg catgcgcacc
     3721 aagctggaag ccgcgggcaa ggcggcaagc gcgggcatcc cgagtgtcct gttctgtgga
     3781 cgcgatccgg ccgtggtcgc ggcgcttggc gccggcgtgc tgcacgggac cctggtcgcg
     3841 gccctcgcca accgccgcag tgcgcgcaag cagtggctgg ggcatgcgcc cgcgtcgggg
     3901 gtgctgcgga tcgatgccgg cgccggcgcg gcgctgctgc gcggggcctc gttgctgccg
     3961 ggaggcgtgc ttgcggtggc cggcgatttc cggcgcggcg acgtggtcga ggtgcgctcg
     4021 gaagacggca tcgtgctggc gcgcgggcag gcgcagtaca acgccgccga tggtgcgcgc
     4081 atcgccggcc gccacagccg cgagatcgaa accatcctgg ggtttcgtta tggcgaggcg
     4141 ctggtgcatc gcgatgacct ggtcctgttg cagcaccccg aagatggcaa ggaggcgatg
     4201 gcatgagcgg atacgtgcgg cagcttgcgc tggcggcgcg tgcggcgcag gcggcggtcg
     4261 cggcctgcga cgccgccacc cggcgcgccc tgatcgtggc gatggcggcg caactggagg
     4321 cccgcctgga cgtggtcctg cgcgcgaacg cggacgacct gcggcgcgcg gccaccgccg
     4381 gtgccggcaa ggccgtgctg gaccggttgc tgctggatgc ggggcgggtg cgcggcatgg
     4441 ccgctgccct gcgcgaggtc gccgcgcagc cggacccgct gggcgcgatc acgcgcagcg
     4501 agcggcgccc gaacgggatc atggtcgagc gccagcgcat cccgctggga ctggtggcga
     4561 tgatctacga agcgcgcccg aacgtgaccg ccgaagccgc ggcgctgtgc ctgaaggccg
     4621 gcaacgcggt gctgttgcgc ggcggctccg aggccgcggc cagcaatgcc gcgatcgccg
     4681 cctgcctgca cgcggccttg cgcgacgttg gcatgcctga ggccgcggtc tcgctggtcg
     4741 ccgacaccgc gcgcgagcac gtgctggaac tgctgcagct gaccgacctg gtcgaccttg
     4801 cgatcccgcg cggcggcgag agcctgatcc gcttcgtcgc cgaacatgcg cgggtaccgg
     4861 tgatcaagca ttacaagggg gtctgccacc tgtacgtgga ccgcgcggcc gacatcggca
     4921 cggcgatcgg cctgctcatc gatggcaagg tgtcgcgccc cggggtgtgc aacgcgctgg
     4981 aaaccctgct ggtgcatcgc gatatcgcag aggagttcct gccgccggcg ctcgccgcac
     5041 tgcgcgcgca cgcagtggag gtgcgcggcg atgcgcgcac ccgggccatc gatgccggcg
     5101 tgctgccggc gacggaagcg gactacgccg ccgaattcct cgacctggtg atcgcggtac
     5161 gggtggtgga ctcgctcgac caggccatcg cgcacatccg ccagtacggc tccgaccata
     5221 ccgaggtcat tgccaccgcg gatcccgtcg cggccgacgc cttcgtccgc gcgatccgca
     5281 gctcggcggt catggtcaat gccagctcgc gcttcaacga cggcggccag ctggggcttg
     5341 gcgcggagat cgggatctcc accacccgcc tgcacgccta cggcccgatg ggcgccgaat
     5401 cgctgaccat cgagcgcttc gtggtgcgcg gcgagggcca gctgcggcag ccgccagcgg
     5461 cgacatgaac ccgcgtgccc gccgtggggg ctggacgcgc ggatggtgcg gctcaatcgg
     5521 atagcacgac aaaccgggcg ggttccggac acgcttccct agacaaaacg ggctatgcct
     5581 tcccgcgaat aggccatggc ccggtcgctt cctagactcc gttggactcc acgcaggccc
     5641 ccgcctgcgt ccggtttcgt ctggggagta ccaccgcatg cctcgcgtcg cgccgaccaa
     5701 actccgtttc gtgccgttcc tgctggcatc gatcctgctc tccacgttgc tgcccgtgtc
     5761 tccggtacgg gccgcatgcg cgccggatcc cgggtcgggc ctgcaggaat ccccgcctgc
     5821 cgcatcggcg caggaggccg ccccggccag cgacctgccg gcgccgcagg caggcgacgc
     5881 cgacgccgac agcgaggcgc gtgccgcgct cgcggcgatc agccggttgg atgatgccga
     5941 caccgcgctg gcgctggccg ccgatggcgc ggcgctgtac gaccgtgaag cggtgaagct
     6001 ggacggctac gcctactgca gccaggcggt ggaactggcc gagaagggcg aattccgcga
     6061 aagcgcccgc gccgccagca aggcgctgca cgtggccctg cagaccggca acaaggacct
     6121 gctgggcaag gcctaccgcg accttgcgat cgccttcaat tacgccggcc agctggaacg
     6181 cgccgagcag ttcgccaacc tcgcgctgaa gtatcccggg gtgaacccga cccaggtcaa
     6241 cggtccggcg cgcaaggtga tcggcgacgt gcgcacccgc cagggcaggt acgacgaggc
     6301 cattgccagt tacgaagagg cgcggcaggg cagttccgac cgtttccgcc cgctggtgga
     6361 ggcatcgctt gcgaatgcgc tgatcctgtc cggcgacctg gtgcgcgcgc gcagcaccct
     6421 ggatgcgctt gccgaacccc aggaggcgaa gcagcgcgcg caattgcagc gcacgcgcgg
     6481 gcgcctgctg ctggccgaga acaagccggc cgaggcgctg gcgctgtacc aggcgctggc
     6541 aagcgcaccg gcggatggcg atgaggactt ctaccgcgcg tgggcgctgg acggcatgtc
     6601 caagagccag gcagcgttgg gcaatgacgc cgaggcggcg caatcgctgg accaggcgat
     6661 cgacgtgttc gaccaggcgc gctcgcagtt ccgcagcgac gaattcaaga tgggcctgtt
     6721 ctccgacctg cagaacgtgt tcgaacgcgc gatcggcatg cacagcaagc ttgggcagcc
     6781 ggcgcgggcc ttcgacatca gcgagcgcag ccgctcccgg gcattgctgg atgcggttgc
     6841 cgggcgcgcc gaactgaagg gcgatggcgg caaggcgctg gacgcagcca gcctgcagaa
     6901 gatgctgcgc gcggacgagg tggtggtggc ctaccacgca ttgccggacc ggctgatggc
     6961 gtgggtgctg tcgagcgagg gcgtgcgcga ggcggcgttc ccggccgcgt tgccgcgaaa
     7021 cgacctggcg cggctggtcg atgcctaccg cgatgcgctg atcaagctga acccgaatgc
     7081 cgcggcgatc ggcgacaaga tcgggcaact gctgctggca ccgctcgggg tcgaagccgg
     7141 caagcgcgtg gtcatcgtcc cgcacgggcc gctgcactac ctgccgttcc aggcgttgcg
     7201 cctcgatggc cagtacctga tcgagcgcaa cccgatctcg atcgcgccgt cgatcagcat
     7261 cgccgcgcgg ctggccgagc gcaccccgag cgtggaggcg cagttgctgg cgttcggcaa
     7321 cccgaccatc gatccgaacg tggcggatcc gctgcccggc gccgagcgcg aggtgcacga
     7381 actctcgcgg cagttcccgg gcgccaagct gttcttcaag gatgaggcga acaaggccaa
     7441 tttcaccgcg aacgccccgc agtcgcggct ggtgcacatc gccgcgcacg cgacggtcga
     7501 tacgctcgac ccgctgcact cgaaagtgtt gctggccgac gagaacggca agccgaacta
     7561 cctggaagcc cgcgacgtga tcggcatgga cctgggcaac gtggcgatga tcgcgttgtc
     7621 cgcctgcgaa tccggcctgg gccgggtcga gaacggcgat gaggtgctgg gcttcacccg
     7681 ctcgttcctg tccgcaggta cctcgaccct gctggcctcg ctgtggccgg tctccgacgc
     7741 cgccaccgag aaactgatga ccaccctgta cgccgaactc gccggcggcg agcaggtgca
     7801 ggacgcgatg cgcgacgcgc agcgcgcggt gatggccgat ccggaaacca cgcacccgtt
     7861 ctattgggcg ccgttcaacc tgatcggcaa ctggcgtttg aaggtggcga aatgaacctg
     7921 ggggctgacc ggatgcgcaa ccaccaacca cggcggatgc cgccgaccaa cctgcttgcg
     7981 gccgcgctgc tgatggcctg cgtgccattc gcgcaggccg cggacggcgc cgccgcgcag
     8041 gaggcgacgc ctgcgccggg gtccgccccg caacaggcgc aggcgctgcc ggagccgcag
     8101 atggcggtgc aggcgcaggc cagcttcgtg ctcaggggcg tgcagttcgt cggtgccacc
     8161 ggggtggcgg acagcgagtt gcaggccgcg gtggccgatc ggatcggcac cagcgtcacc
     8221 ttcgccgacc tcgagcagct cgccgcgcgc gcggtcgcgg tgtaccagaa gcgcggcttc
     8281 ggcctggtcc aggcgtacgt gccggtgcag gaagtggtcg atggcgtggt caggatcagc
     8341 atcagcgagg gcgtgctcgg caacgtcagc atcgagctcg ccgagggcgt gccggtcgcg
     8401 caggaacggg tggcggggac gctggcgatc ctggagcccg gcaagccgct caacggccgc
     8461 cagtacgaac gcgcgatgct gttgctgtcc gacctgccgg ggatcaagcc gcagtcggcg
     8521 atctccgccg gcgcggccac cggcaccacc gacctggtgg tgcaggtcgg cgaacgcgac
     8581 cgcctgcaat acgggctgga agtggacaac catggcacca gcgattccgg tcgccaccgc
     8641 atcaccggca gcctgcgctg ggccagtccg ttcggacgcg gcgacaacct cgacctgcgg
     8701 ttgatgctgg cggagggcat gcacaccgcg ttcgggcgca tctcctatga aaccccggtg
     8761 gggtaccgcg gcctgcgcat cggtggcggg ctggcgcgcg tgcaatacga gcttggcggc
     8821 ccgttcgcgc cgctggaacc gaccggcacc ggcaacgtcg ccgacctgtc cttcagttat
     8881 ccgctcatcc gccagcgctc gaccaacctg ttcgtgcgcg gggtgatcga caacaaggac
     8941 ctgaccgacc gcttcgaggc ggtgggtttc gaaacccaca agcgcgtccg cggcgccggc
     9001 atcggcctgt cgttcgaacg ccgcgatggc ttgcttggtg gcggctacac cagcgccaac
     9061 gcgctgctgt accgcggcaa cctcgacatc aaggacgcca ccagcgaggc cttcgacaat
     9121 cctccgttcg ggtacgacac cgagggcagt ttcggcaagg cgaccctgca gctggcgcgc
     9181 ctgcagtccc tggcaccgaa attcagcctg ttcctgggca ccggcatgca gcgcgccagc
     9241 aagaacctgg acgcgtacga gaagctctcg ctgggcgggc cgaaggcggt gcgcgcctat
     9301 gccaccggcg aggtgctggt cgacgacggc tggctggcga ccgtggaact gcgctacgcg
     9361 ccacgtcctg ataccaccct gttcgccttc tacgacgcgg cgcacggcga cttcttccac
     9421 gatccgcggc cgttcgacgt gttcaccagc cgcagcctgc gcggccatgg cctgggcctg
     9481 aactggagca ggccgggcaa gctcagcatg aatttcagca tcgcctggcg cagcaccgac
     9541 cccggtgtca ccgatggcgg cgaccgcaac ccgcgtttgt tctggtcgat ccagaaggcg
     9601 ttctgaggag tacgagatgc gcaccaagct gacttccaag caactccgct gcctgcgccg
     9661 ccggcgcctg gcgctggcgc tggtcggcgc gatggcgctg ccggccgcgg ttgcccagaa
     9721 cctgcccacc ggcggcaacg tggtcaacgg caccgccagc atcaacgaag ccgggaacca
     9781 gatgacgatc acccagacca cccggggcgc gatcatcaac tggggcacct tcaacatcgc
     9841 caacgggttc ggcgtggcgt tcaaccagcc gggcaacggg gtcaccctca accgcgtgct
     9901 gggcaccatg ccgaccgcga tcaacggcat cctgaccgcc aacggcaacg tgttcatcat
     9961 caacaccaac ggcatcacct ttggcgccaa cgcgcaggtc aatgtcggcg ggctggtggc
    10021 ctcgaccctt gccctcagcg atggcgattt cctcaccggc ctgtcgaccg ggcgctacct
    10081 gttcgccggc aataccggca cgccggtgct cagcaacgca gccgacatca ccgccggtgc
    10141 cggtggcgtg ggtttcgtcg gcaccaactt gcgcaacagc ggcgcgatcg tcgccaacgg
    10201 cggcaacatc ggcttcggcg cgggcacctc ggtcaccctg gatttcgtcg gcgatggcct
    10261 cacccaggtc accatcaacg cggcgcccgc ggtggacacc cagctgttcc aggaccgctt
    10321 cggctcgatg accgccgatg gtggccgcat cctcctgcgc aacgccgcca ctgccgccgg
    10381 caccggtggc gtgatctatg ccgacggcct gttgcgcgcg cagagcatcg ccaacgtcaa
    10441 tggacgcatc gagctgacct ccatcggtgg cccggtgttc ctgggtgcgc caggggtact
    10501 gtcggacgcg tcgaccccgt tcacggctgg ccgcatcgac gtcagcggtg acgttggcga
    10561 gaccggcggc tcggtgctga tccagggcaa cggcgttgcg ttcctcaaca acgacccgac
    10621 gccgttggcg cccgccgatc cgctttcggt cggcagcttc atcaatgcct ccggcggtga
    10681 aggcggcggc agcgtcacca tccagtccac cgccgacgtg accatgctcg ggctgtcggg
    10741 catcagcgcc gatacccagc gcggagtcgc cggccaggtg ctggtgcagg cggtcggaag
    10801 catcggcatg gccgcggcca cggtgatcag cgcggatgcc atggacgccg gcgacggcgg
    10861 ccaggtgggc ctggcggccg ggggcaatgt cgtggtggcg ggcctgttgt cggccaaggg
    10921 ggccaagggt ggcggcaacg gcggcacggt ggcgatgtcg tcgcagggtg gagccgcggt
    10981 gctgcagggt ggcatcgacg tgcaggcgtc gcagggcaat ggcggcgacg tcctgatcgt
    11041 gggcgaaacc gcgtccgggc tcgccaacgg cgcgtccgtc aatgccagcg gcggcatcga
    11101 tggcggcagc gtggtggtgt ccgccaacaa cggcggggcc gtgctgggcg agaccgcatc
    11161 gatcgatgcc cgcggcttgg gcggcaacgg cggccaggtg gccctggccg gcaccagcgt
    11221 ctacgcctat ggcgcgatcg acgcgcatgg cgcggtgggc ggcgggctgg tcggcaccat
    11281 cgcgcgcggc ggcgacctcg acctgcgtgg cctcgacgtg gatgccggtg gcggcagcac
    11341 ggcaggcatg tggatgatcg gcggcgcctc gctggaggtg gtcaacggca acgaccaggg
    11401 cagcctgacg gcgacccagg ccggcagcag cgtgcaggac gccgacatca acgcggcgct
    11461 ggacaccggt accagcatcc agctggtcac cagcgatggc ggggtcaatt tcgatgacgc
    11521 caggatcagc accagctcgc cgctggcgtt gaccttcacc gtgaattccg agtcttcgat
    11581 caccggtggc acgttcgaca tcgagtccac tggcggcagc ctggcgatgg acttcaatgc
    11641 ggatgccgat ggcgacgatc caggcgaagg cttcatcgcg ttcctcggtg ccaacctgtc
    11701 cagcaatggc ggcgacatcc gcttcttcgg cgggtccgac ccattggccg gggccgccac
    11761 cggctacggg tccgggatcc gcctcaaccg ggtcgcgatc gacaccgccg gcggcgacgt
    11821 gctgctgcgc ggtgccagca ccggcgcgga caccggcccg gatgatgccg gcgtcgccct
    11881 cgagttcagt tcgatcgaca ccgacggcgg cgacgtggtc gtcgagggca ccggcgccgg
    11941 cgataccggc ggcgtgttcc tgtggagcgg acgcatcgac gccagcggcg gcggtggaac
    12001 cggtgcgatc accatcaccg gcgccagcgc ggcggcgttc aatgcggtcg acctggacgg
    12061 cttcgggctg ctggccgatg ccggcgcgat cagcctgcag ggcgatgccg gtgccgatgg
    12121 caccggcgtg gcgctgcgcg cgagcctgct ggacagcaat ggcggcgcga tcagcgtggt
    12181 cggcagcagc gtggccgggg ttggcctgtc gttcgccgag ggttcggcgc tggccagcgc
    12241 gggcggcgcg gtcctgctgc agggcgatgg cgcgctggcg ggcgtggagc tgctggacgg
    12301 cggcatcgac ggcggcggtg gcgacatcac cgtcatcggc tcggcatcgg cggcgaacgc
    12361 gatcggcgtg tactcggacg gcgtcgacct cgtcggcggt gccggcgacg tgaccgttga
    12421 tggcgcgtcc ccgctcggga tcggcgtgct gttcgcgaac ggggcagggg tgagcacgac
    12481 cagtggcgcg atcaccctga tcggcaccgg cgcgaacttc ggcctggaca tcgccgatgg
    12541 cgcgatcgac accgacagcg gcgacatcac cctcagtggc gaggcgctgg ccgccaccgc
    12601 caccgcaggc gtccgcgtga ccggcggcgg cctcgccacc aacggcggca gcatcagcgt
    12661 gagcgggacc agcgccggcg gcgtcggcgt gcagctgggc gacggcggcg cgttcgcgat
    12721 cggcagcggt ggcggcgcga tcagcatcaa cggcagcggc gtgaccgcgg gcgtgctgat
    12781 gcagggcaac acggtcgcca gcggtggcgg cgtcatcgac gtgatcggcg cggcgtccgg
    12841 cggcaacggc accggcgtgg accttgccaa tgcgcagctg gtcgccgggg ccggtgacgt
    12901 gcgcgtgttc ggcaacgcgg ccgccgggac cggcgtgaac ttcgccggcc agtccgggat
    12961 cagcaccacc accggcgcga tcgcggtgac cggcatcggt gccacggtcg ggctggccct
    13021 ggccggtggc gaactgagca ccgaaagcgg ccacttcgac ctgcgcggcc gcgggaccgg
    13081 tgccggttcg gatggcctgg tgatcgggca gggcgtgtcg atcgccacca atggcggcgg
    13141 catcgaactc agcggcgaag gcggcagcgg cgcaggcgtc agcctggagg ccgggtccag
    13201 cgtcgatgcc ggcaacagcc tggtggtggt gcgcgccggc aacgatggca gcagcgatgc
    13261 gctgcgcatc gccggcacca tcgccagcgg gatggcggtc aacctgcgcc cgggcggggt
    13321 cgacgcgaac ggcggcctca ccgaacgcac ggccgacgaa atcctgattg gcggcggcag
    13381 tgccggcttc gccctcagcg gtgccgaact tgcgctgatc gatgcgccgg aactgatcat
    13441 cggcagcaac ctgcatgcgg gtgcgatccg ggtgctgggc gcgaccagcc gcagcggcga
    13501 cctgaccctg cagaacgacg gcggcagcgg cggcatcgac atccaggccg cactcgacgt
    13561 cggcaccggc accctggcgt tgtccagcgg cggcgacatc acccagtcca gcggcggcgc
    13621 gatcaccgcg cacagcctgc tggcgcgcgc cggcggcagc gttctgctgg cggacgccgg
    13681 caacaacgtc gcggcgacca ccctggccgg ctcggccggc ggcgacttcg aatacgagga
    13741 cgtcgatgcg ctggcgatcg gcagcgtctc cggattgggc atggacgcct cgtcgaacgg
    13801 actcgccagc ttcggggcca ccggcatctc cgccggcggc gacgtgttcg tgcgcaacct
    13861 cgccggcgac atgacgctga acgcgggcgt gtccggggtg aacatcgacc tggtgaccgc
    13921 gaacaccctg cagaacgcgg ccggcgccag cctggtcgcc agcggcgact ggcgggtgtg
    13981 ggcgaacagc tgggtcggcg aggaccgcgg cggcctggcc ggcgacggca acctgccgaa
    14041 cctgtacggc tgcgcctacc tcggccagtg cggggtgacc gtgccggccg cggacaacca
    14101 cttcatctac gtgcagcagc cgaccgcggt gatcaccttc gacgatttca tgcgcgaata
    14161 cgggctgccg aacccgacct tcaccttcac cgtcagcggc gcgatcctgg gcgacagcgc
    14221 ggccaacgtc gcctccggcg tgggcgtgac caccgcgacc atcggcagca acgtcggcaa
    14281 ctacccgatc accgggcagt tcacctccgc cgccggttac cgcctgcagt tcgtgcccgg
    14341 cacgctcggg atcaccccgg ccaccctgct gttcaccgcc gacagcttca tccgctacct
    14401 cggcaccgag aacccgctgt tcaccggcag cgtcaccggc ttccgcaatg gcgacaccat
    14461 cgagagcgtg ttcggcagcg gggtgatctg gaacagcccg gccggcctgc tttcgccgat
    14521 cggcttctat ccggtcaacg gcggcagcag cgcgaccaac tacgtgttcg tgcaggcacc
    14581 gggcaacgcc accgcgttgc agatcatccc gctgccgcaa ctgtcgtcga ccccgatcga
    14641 cctgatccgg gaaacggtca acacctacgt ctacgaccgc aacttcggcg gcgccccgat
    14701 gtgcgcgctc aacgcctcgc tggaagacca gcaactggcc tcgtccggcg acgagttgtc
    14761 gaacgaatgg gcgaaggtgc gttcgcgccc gaacctgacc aactgcttcg agaccgagcg
    14821 caagaacagc tgcggcgatt tctgagcccc ggtcgcggcg cctgcctgct caggcggcgc
    14881 cgcgcatgga ctgtggcagc ggacgctgcg ccagcagcga gcgcagcttg gcgggggcgg
    14941 gtgggcgcga cagtgcctgc gcgccgtgtc gcgccgccag ttccagcagg cccggccccg
    15001 gggatgcgca cagcacgatg cgcgcggcct gcgcctgctg ggccgcagcg gcgcggagca
    15061 ggccccaggc cgggtcgtcg tcggcatcga tcgcgcacaa caccgcatcc agcccgccat
    15121 tgccgccggc aaggtgcgtg catgcggcat cgaccgccga ttccgcctgc accgcgtagc
    15181 cccaggaccg cagcatcgcg gccagcgcat cgcggtgccc ggggtcgcga tccagcagca
    15241 ccgcgcgcgg cggacgctgc agcaccgaag cgcgcggcag cgccagccag aagcaggtcc
    15301 cggcatgcag ccgcgagcgc agttccaccc gggtgccgag cagggcggcg agccgggaca
    15361 cgatcggcag gccgaggccg aagcccttgc ggtggtcgcg ctcggggttg ccgacctgga
    15421 agaattcgtc gaacacgcgt ggctggtgga tcggcgcgat gccgacgccg ccatcgcgga
    15481 cctcaaggcg cacctgggta ccgtgccggc gcgccgccag cagcacccgg gcgccgcccg
    15541 gactggcctt gatcgcgttg tccagcaggc gttcaagcag gctgccgagc aggcgcgggt
    15601 cggtgaacac ggcgtggcca ccggcgtgcg catgcagcga cacgcccgcg cggcgggcgg
    15661 catccgcctg tgcagccagt gccgaggcga acgcctgctc cagctcgcac gcgctggcgt
    15721 gcgcctgcag cgcaccggca tcgagttgcg agagcagtgc aaggccatcc agcacctcgg
    15781 cgagggtgcg catgccggca tccaactgga cgagttcgcg cgcctgcgca ggcaggggtt
    15841 cccgccgcaa ctgcgccagc gccaggtcca tcgtctgcag cggctgccgc aggtcatggc
    15901 tgatcgcgga caggaaccgg tccttggcgc gcatcgcgcc atcggtctgc tgcatctgcc
    15961 ggaccatgtc cgccgacaac tgccggcgct gcgcatcgag ttcgtcccgc gcctgctgca
    16021 tcagcgcgat ccgctgcgac agcgcgtcga gcaagggggc cagcgatggc gccgcaaccg
    16081 caggcggcag gttgcccgcg gcgacctcgg cggccagccg gtgtgcctgg cgcgcctgcg
    16141 cacggcgcag caggatcgcg gacacggccg cgcccaacag cccgccaccg atggcgatgc
    16201 ccgacgccag cgcaaattgc tggatcgctt ccatgaatgc cccaggggac gcgcgttcag
    16261 gttaggcacc ggcgcacgcc ccggcgatat cccatcggcg ctagccgccc cgcctcggag
    16321 ccacgccacg cggcgtctgc actaggccga acgtggtatc gaaatcgacc ggatcgcggc
    16381 gcagactcaa ccgcgaggat cggcgaatgc cggccgccgc gcggcatgcc gccgccggaa
    16441 tgcatcctgg ccgcgaggcc gcaacgctac gaggacaacc atgagcacga ccacgattct
    16501 ccgctccgca ctggcgatcg ccctgctggc cgcgtgcgcc ggcctgcacg cgcaggatgc
    16561 gcaggatccg cagagcgcgc cctcggtgga ttcgatcatc gagagcctga aggccgacgc
    16621 ccccgcccag caaccgggcc agacccgcgc actgcgcccg ggcgcggcgt cgatgtcgac
    16681 cgcgccggtc gccgcgccgg ccaagccgaa gccggcatcg atcaacatgc agatcaactt
    16741 cgagtacaac tcggccgaaa tcgccggcag ctccgagaag accatggcca cgctggccaa
    16801 ggcgctggca tcgccgcagc tggagggccg caagttcacc gtgatcgggc ataccgacgc
    16861 cagcggctcg gcggcctaca acaaggccct gtccgaccgc cgcgccgccg cggtgcgccg
    16921 ctacctgacc gagaacggcg tgtccgccgc gcgcctgaag gcgatgggca agggcgagag
    16981 ccagctgctc aacaccgagg atccgaacgc gggtgaaaac cgccgcgtcg agatccaggc
    17041 caccggcggc tgagcattcg acgatgccga tgcaggtcgt caatggcgcc gtgctgatgt
    17101 gcagcttcgg catggcgcca tccaacctgg tggtgttgcc ggtcaatcgc gtgctgtccg
    17161 gcaaccagcc cgcggcgaac atcatggacc acaagccgat ggtgaacatc ctgccgttcg
    17221 ggatgtgcat caccccgtcg aacccgcagg tggcggccgc caccgcggcg gcgcagggcg
    17281 tgctgacccc gcagccctgc atcccgatga cccagacccc atgggtcccg ggcgcgccga
    17341 ccgtgctgct cgccaacatg cccgcggtgg acaacacctg cacctgcatg tgcctgtggg
    17401 gtggcgtggt cagcgtgacc agcccggggc agttcaccga aatgattcca tgacgccact
    17461 gcggtagcgg ttccgggaac aggcggcgat tgccgccttt ttcgtgtcgc ggcggttcag
    17521 ccgaacaggt tgccgagcga ttgcgcggcg ttgcccatcg cacccgcggc acccgcgacg
    17581 gcggactggg cggcaccgac tgcgccctgg gcggcctgtc cggccgcggc ggacgcctgg
    17641 ccaatggagg actgcgcgct ggcgatcgcg ccctgggcgg cgtcgccggc ctgggtggcc
    17701 gcggcctggg cggcgttctg ggcgttgttg agtgcgccct gggcggcgcc gctgagcgcc
    17761 tgctgtgcct gctgcgcggc ctggttggcc tgcgccaggc cttgctgggc ggcctggttg
    17821 acctgctgct gcgcctgcgc ggccgcggcc tgggcgcctg cgatcgcctg cccggcggca
    17881 tcgccggcct gctgttgcgc ggaatccacc gccgccatgg catcgccgat cgcaccctgc
    17941 gcggcatcct gtgcctgctg cttcatgcca ttgaccgcat ctttcacctg tgcgatcgct
    18001 gcgccggcct gttcgcggac ggcctgctgc gcctgctgca cggcggtcct ggcggcgcca
    18061 agtgcctgct gggcggcgcc gttggcggtt gcctgggcct ggtcgagcgc acccatcgca
    18121 tcatccatcg cgcccagcgc ctggtcggcc agcccgttgg cctggtccag cgcattgctg
    18181 gcctggtcca gcatcggccc ggcgatgtcg ttcagcgccc cgaccgcatt gccaagtgca
    18241 ttgttggcct gggccgcacg ccgcgcgagt tcgtcccgaa gcctgttcac gtacgggcaa
    18301 tcggaggccg ggaaatccac cggcaggttg gggaagctgg tcggcagcgc atccagcgcc
    18361 gccgcaacct ggcccagcgc gccgcgggcg ttgtcgatca ccgcgctgcg tgcctgctgc
    18421 agcgcgttgg tggcggcgtc gcggagctgc tcctgggcct ggtcgatcgc gttgttggcc
    18481 gccgccagcg cgttgccgac gccgtcctcg agcatgccca tggcgttgtc gaccatgccc
    18541 atggcgttgt cgatcgccga ttgcgccgcc tgctcggcgg tggccatcgc gttctccagc
    18601 gcacccgtgg ccgcggccaa cgcaccatcg gcgatcgcct tggcctggtt ctgcgcggta
    18661 ttgaccgcgg actgggcctg ggccaacgca ttctgggcgg cggcgccggc ctgctggctg
    18721 gcatcggtaa gcgcgttgtt ggcctgggcc agcgcgttct gggccatgtc gttggcggcg
    18781 ctgcgtgccg catccatggc ctggttggcc tggccaagtg cgccctgggc cgcggccccg
    18841 gcctgcgcgg cagcctgttg cgcggcctgc tgcgcctgcg cgaccgcgcc attggctgcg
    18901 ccggcggcag ccgccgacgc attcgcgacc gcggtctgcg cttcggagat cgcgccctgc
    18961 gcggcattgg cggccgcacc cgccgccgcg gcggcctgtt gctgcgcctg ctgcacggcc
    19021 tcgccggcgg cggcaccgat attcgacagg ccttcggtca gtccctcgaa catgtcagcc
    19081 tccctgggtc cgtcggtagt cgccataggc ggccgcggtc cacatctcga tcagcagcgc
    19141 gcggtcgagc tgcgaactgc tgcggtccgc cagcaccccg atgatcgagg ggtcgccggc
    19201 cacgcggtcg ttgccgaaca gcgagcaggc cagcgcatac gaggcgaccg cgcgctggct
    19261 gcgcagtccg gccttgcggc aataggcgat gatcgcgtcc agctccgcgt ccaggcggtc
    19321 gagcggcagc gacgacaacc cgtccacgtg gcggccgagg aaatccgcga ggttgttgcg
    19381 aagctgggct tcgccgatcg cggccatctg tttgcgcttg actgcgagca cctggttggc
    19441 tccttcaggc cgcggacgca agcggcaacg cctgcggctc cggcctgcca tcgcgggcga
    19501 agaacagcac ggagtcgggg ctgcgaccct ccacgaaaac gccgtggatc gggccgaaga
    19561 actcccggcg agcaggcggg tccagcaccg ggatgacggt ccggaacgcg cgcgggtcgt
    19621 agaagcggaa cagcatgctg gcgccatccg gcgcgcgcac gcgcaggacc cggcgcaggt
    19681 gcgaacgcac cgcgcgcagg tcgagcgcgg ggtcggtctg caacacgatg ccccagtggc
    19741 cgttccagcc gtcgcgcaat gccatctggg tggcggtgga gtccgggtcc agtttcacca
    19801 gccacggggc ggcggcgctg agcatgggat ccagcgcccc ttcgaacagg caggcggcat
    19861 cgggtgcgat ctccgccagc cgcccgggca ggccttcgat catggcgccg tccatgaccg
    19921 catagacggc ccgcgcacgc tgcccgaaga ccagtttgtg cagcgattcg tattggcctt
    19981 gcgggatcac ctggtctcgt ctccgagcgt tcatcggcac ggcgcgtggc gtcgcatgcg
    20041 gcccggggcg acatgcggcg gccgttgcgc accatggccc gggcgacgga cagcttgcgt
    20101 ccccatgcca tcagtcatac gtgacacggc ggtgaaacag gtcatgccat cagcgccgcg
    20161 taggcggcgc gggcggcttc gcattcggcg cagaacggct ggccggcgag tgcggcattg
    20221 cgcagcgcct gcgcctgcaa ctgctggccg gggtcggtga cctggccatc ctcggtgcca
    20281 tgggaaccct gcgccttctc ggcgctgaag ccggacggtg cactcgcggc actgggcgcg
    20341 ccgccggagt tctgcttgat gatcggcccg atcacgctga ccccgctggg gtcgatcacg
    20401 atgccgctgc cgccgacctt gaggctgatc agcgtgttcg cctccatcac cagcttcatc
    20461 ccgccatgga tgtcgatgcc gcccctgctg ccgatccgga ccgaggactt cccgagcagg
    20521 cgcaactcct gctccgaaag cagttcctgg tcgcccttgc tgccctgcat caagtcggcc
    20581 ttgacgacca tggtgctggc gccatccacc ttgaggtggc ggtcgccgcc gacgtatatc
    20641 cgctgcttgc cggcgaggtc gtggttggaa tcgccgccga ccgattcggt gcggttggcc
    20701 ttgaccttga cgttgaggtc cttctccgcc tgcaggaaga cttcctcgct gcccttcttg
    20761 tcctcgaaga gcagctcgtt ggtgccgcca ccacccttcg aggacaaggt cttgatgccc
    20821 gacttggtcg ccgggccggg gcctgggggc gcatacggtg gccggttgac gccgttgtag
    20881 acgcaaccgg tgacgatcgg ctggtccggg tccccctcca ggaagtcgac caccacctcc
    20941 atcccgaccc gcgggatgaa catcgtgccg aagccgttcc cggcccaggt ctgcgccacc
    21001 cgcacccagc acgagctgcg gtcgtcgtag cgtcccaggc ggtcccagtg gaactggatc
    21061 ttgatccggc cgtattggtc ggtgtggatc tcctcgcccg cggggccggt caccagcgcc
    21121 gtctgcgggc cgcgcaccag gcccttcggc gtggacagcg gggcgcgcca ggtgacctcg
    21181 tgcggttgca ggctgaattc gttggcgtag tccgatggcg cgccattgcc ggtcaggttg
    21241 ttgcggccgt ggtgccagac cgacagcacc atgtattcga tgttgaattc atcgaagggg
    21301 tggtcttcca gggtgaaggt gtggccgggc gccagcgtgc ggaccttgcc gttgccacgg
    21361 aagcgcatgt ggctggattc ctccgcctcc atgatcacgc gcgaacggta cttgccgagc
    21421 gccggctcga cgaagccgcc cgggtagcgg tagcgctcca ggtcgcggtc cgcgccgatc
    21481 gacagcacgg ttggcgtgtc ttcgcgcagc aaccgcttgc gcacgaagtc ccagtcgcca
    21541 agggtgacct tgcgggtgcg catctgctgg tccagggcca gcgcctggat cgcgtcgaat
    21601 tcctcggtat ggccttcctc gtcgaacggg accgtgtccg ggtccagcgc cgggttgctg
    21661 ccgttgttgt cgctgaacac cagcaggtgc cgggtttccc cgccttcctc gtagtggcgg
    21721 aagtagtagt ggatgccctc gcgttccagc agccgcgaga tgaagtcgaa ggtggtctcg
    21781 tggtactgcg tgcagtactc cagcgacggg tggttctcgg tcaggtcgaa gcggaaatcg
    21841 gtgtaggcga actcttcgaa gatggcctcg acgatctcgc ggaccgtctt gttctggaag
    21901 atgcggcaat cctcgtgctg ggtcaggaac cagaaccacg gcacgatgtc gcattcgtag
    21961 gaatacacgc tgtcgccatc cggcgaggcg gtgcggccgg tgcgctggaa gcgtgagacc
    22021 accccgttcc agtggcgctc gccacccatg tggtggatgt cgcgggtctc ggtgcgcagg
    22081 atcgccggct tggcgatgat cctgcgcggg tcgatgtcgt cgcgctcgga cagcaggcgc
    22141 aggcggaact ggaacagctg cgacatcgcc tcctgtccgg tcatttcctc gatcagcagg
    22201 tcgtcctccc cgtagtcgtg cagggtgacg aagtacatcc ggttgctggt ggaaaggctg
    22261 gagggcatgg tcgtggtcgc ttgtgctgcc tatcgtcgtc ggtcgcggcg ccgcgcgaca
    22321 ttcgtccgaa gtgctagccg cggcccgtgg gccaggccgt ggatagcgcg aagggcctag
    22381 tgctgttggc gtcaggtcac cgcgtacgcg aaaccgccgt ccccgccgac cgcgatccgg
    22441 atctcgctga tcgtgccgcc ttcggccatc cgctccagca cgtggccgga cacctccggc
    22501 agcaccgtcc cggtcaggat gttgtcgatg ttgcgcgcgc cggaatcgac ctcggtgcag
    22561 cgcctggcga tggcgtccac caccgcctcg tcgtattcca gggtggcgcc gtggttggcc
    22621 ttgatccgct tgccgatctt gttgagcttc aggcgcacga tcttgcgcat gttctcgtcg
    22681 cgcagcgggt agtacgggat caccgcggtg cggccgagga aggccggctt gaaggtgcgg
    22741 ttgagctccg gcttcagcaa ctcgttgagg taggccaccg tcggcagttc ctcggactcg
    22801 ttgcaggcct gcatcaccag gtcggtgccg gcgttggagg tgaggatgat gagcgtattg
    22861 cggaaatcga tctcgcggcc ttcgccgtct tccatgcggc ccttgtcgaa cacctggaag
    22921 aacagctcca gcacgtccgg gt
//
LOCUS       DAORUK010000016        22940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_1825_length_22940_cov_18.716976, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000016 DAORUK010000000
VERSION     DAORUK010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 22940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22940
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1825_length_22940_cov_18.716976"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PK818_02060"
     CDS             <1..740
                     /locus_tag="PK818_02060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQY81551.1"
                     /translation="VHGYVVKPWNYQPGRKYPVAFLIHGGPQGSFGDGWSYRWNPQTY
                     AGLGYAVVMIDFHGSTGYGQAFTDSISQHWGDRPLEDLQKGWAAAQAKYAFLDGDKAC
                     ALGASYGGFMVNWIAGNWPQPWKCLVSHDGVFDQRAMGYATEELWFTEWEQGGTPYDK
                     PANYERFNPVNHVKDWKVPMLVIHGQLDYRIPVEQGIAVFTALQRRGIESKFLYFPDE
                     NHWVLKPQNSVLWHDTVNGWLQQHIGQ"
     gene            760..2010
                     /locus_tag="PK818_02065"
     CDS             760..2010
                     /locus_tag="PK818_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HQY81552.1"
                     /translation="MNEASRTVLIGNDAAVLGLLAMILGFVFWGASRERGFWKKFYAV
                     VPAILPCYLLPAIANSTGLVDGAGSGLYPMARDYLLPASLVLFCVSMDLKGIVRLGPK
                     ALVMFLTGTVGVMLGALVAFEAMRFIHPATVAGETWRGMTTIAGSWIGGGANQAAMKE
                     VFAVDATVFGQFAAVDVIVAYLWMAVLLFMASRSAAIDRWNRADTSAIDDLRRRISDY
                     QAQHARVASTTDLMLIFALAIGITGLAHLLTDPLVAWVRAAPAAWRLQDYSLDARFFW
                     IVVFATTFGVLLSLTRARQLEGAGASSVGSVFLYILIATIGMQMDLKALADKPLLLLL
                     GLIWIGVHGGLMLLVARLIRAPLFFMAVGSQANIGGAASAPVVANAFHPALAPVGVLL
                     AVMGYALGTYCAYLTGLALRALAA"
     gene            complement(2024..3022)
                     /locus_tag="PK818_02070"
     CDS             complement(2024..3022)
                     /locus_tag="PK818_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016849505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HQY81553.1"
                     /translation="MNTPATALPACVVNCAAYDRDGHRRDISLDAVSDVLAVDDGSFV
                     WVGLYEPEEGLLEKMQEEFGLHDLAIEDAQHAHQRPKLEAFGNSLFIAVHTAQRIDAH
                     VRFGETHLFVGPRFLVTVRHGASLTFSIVRDRLERDPAALAHGPSVAVHAVLDFIVDN
                     YQPIVSDFEDELDMLEQDVFAETYKRGTIRRLYDLRKELTRMRMAVAPMQDILSQLIR
                     TPVVAIPDEVKPYFRDVLDHVARVGDSIDTLREMVSAAMNVNLSLVTVAQGEIVKKLA
                     GWAGLLAAPTLVTSWYGMNFRHMPELHGRYSYAALIGGVALSCVLLYRYLRKIRWL"
     gene            complement(3054..4250)
                     /locus_tag="PK818_02075"
     CDS             complement(3054..4250)
                     /locus_tag="PK818_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HQY81554.1"
                     /translation="MRITEALRFALLLALALSAGRVHAQAADTGQPPRIGVVTMGPGD
                     VFWERFGHDAIVVDDPARGEPISYNFGFFDLAEDGFIGRFVRGEMQYRLVALPLDDDM
                     QYYREVGRGASLQWLALEPAQARQLAADLAENAKPENARYRYDYFTDNCATRVRDALD
                     RALGGNLRRQMEPRSSGDSYRSESVRLASPEPWMWLLFDVGLGPYGDRPLSRWEQAFL
                     PRRLADDVRDAKLPDGRPLVVAEQQLLPQRQPAEPDERPRHAWPWLALGLALAASILA
                     CARRAPRLLGGVGLGFWLLCGAVGLVLLLGWGFTAHRALWANRNLLLFNPLCLLLLPG
                     GWALLRGHAGSGRFRSLLVAVAALAAVALLPLWLQAMPQRNGPWIALLLPVHAAFAWC
                     RGRRKP"
     gene            complement(4237..5118)
                     /locus_tag="PK818_02080"
     CDS             complement(4237..5118)
                     /locus_tag="PK818_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HQY81555.1"
                     /translation="MSEDDSRHETADSAERSADKPRRSWLDRISAALSGEPTSREDLV
                     ELLRDVQADGLIAADTLRMMEGAIAVSDMSVGDVMVPRAQMVALPADARFLDLMKEVV
                     ESGHSRFPVHGEDKDEILGILLAKDLLRGVVADNGPGTIRELLRPAVLIPESKKLNVL
                     LREFRQSRNHMAIVIDEYGGVAGLVTIEDVLEQIVGDIDDEHDEAEDGSTLIAAQADG
                     QFVVDALTPIADFNERFGADFDDDEYDTIGGLVTNAIGHLPEAGEELTLGRFVFRVAR
                     ADARRLHALHVGVHADH"
     gene            complement(5189..5671)
                     /gene="ybeY"
                     /locus_tag="PK818_02085"
     CDS             complement(5189..5671)
                     /gene="ybeY"
                     /locus_tag="PK818_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016901623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HQY81556.1"
                     /translation="MTKGPVRLDVAVGYAVPRAGIPAAVSFRKWVAAALAGRIREADL
                     AIRVVDEKEGRAFNHHYRGKDYATNVLSFPAELPEGLPEGVRLPLLGDLVLCAPVIAK
                     EAAAQGKPLAAHYAHLTVHGTLHLLGWDHEDPREAECMEQLERGILAGLGLPDPYIER
                     "
     gene            complement(5682..6671)
                     /locus_tag="PK818_02090"
     CDS             complement(5682..6671)
                     /locus_tag="PK818_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQY81557.1"
                     /translation="MQTQRDFTLEPDDLERLANLSGPFDAHLRMIELRLGVEIANRGS
                     VFRVSGRDGDAVARGERLLRELYQDAASETLDEHAIHLRLGAYARDDIPAVQAPDHVP
                     QEISVRVKRGNVRGRGPNQQNYLHAIASHDINFGIGPAGTGKTFLAVAMAVDALNQSR
                     VQRLILVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYEMLGVEKVARLLEKNVIE
                     IAPLAYMRGRTLNDAFVILDEAQNTSIEQMKMFLTRLGFGSTAVITGDMTQIDLPKHQ
                     KSGLKDAVDVLRGVDGVHFTFFESRDVVRHPLVARIVNAYEARDAKDGQAGSA"
     gene            6774..8447
                     /locus_tag="PK818_02095"
     CDS             6774..8447
                     /locus_tag="PK818_02095"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQY81558.1"
                     /translation="MRKLAWVVSAILLAAAGSPAAQDGVTLVTASRIHTMDTARPMAT
                     AMAYGADGRILAVGQADALAKQYPRARRVDVGNATVVPGLIDAHAHIGGLGLAMLSAD
                     LVGTRDKAGILQRLRVHEQTLKPGEWLVGGGWDQNDWPQQRFPNAADLDAAFPDRPVW
                     LSRVDGHAGWANSAAIRAVRRDLSGDWQPDGGRIDRDAAGKPTGLFIDNAMLLVEQAR
                     PAMSEATAEHALEIAMRAAAEQGLTGVHDAGITLAELGRYQRLADRGRMPLRISAMAD
                     GDSAALESLCRNGLYRHPSGRLQMRAVKLYADGALGSRGAAMLEDYSDDHGNRGLMVM
                     SPAQLAAATAKAKRCGVQVATHAIGDRGNREALDAYATALGTDAASDHRWRIEHAQIL
                     SAQDLPRLAGLRVIASMQPTHATSDMPWAEDRVGAQRIAGAYAWRQLRDSGARLALGS
                     DFPVERVDPRLGLQAATTRADGEGKPVGGWFPGEKLTAFEALRGFTLDAAHAGFAEAE
                     VGSLVAGKRADFVVLAADPLASPEARLKEIAVLATYVDGKPVFEAASGR"
     gene            complement(8463..9842)
                     /gene="miaB"
                     /locus_tag="PK818_02100"
     CDS             complement(8463..9842)
                     /gene="miaB"
                     /locus_tag="PK818_02100"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HQY81559.1"
                     /translation="MSAETTEAAPATGARGKLYIQTHGCQMNEYDSAKMADVLAAADG
                     MELTTIAEEADVILVNTCSIREKAQEKVFSQLGRWKSLKKDGRPVLIGVGGCVASQEG
                     AAIVKRAPYVDLVFGPQTLHRLPELIRDRRASGLPQVDISFPEIEKFDRLPEPRADGP
                     SAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREVNLLGQNVNA
                     YRGPYGEGEFADLGLLIRAIAEIEGIGRIRFTTSHPLEFSDALVEAYRDVPKLANYLH
                     LPVQSGSDRILAAMKRGYTALEFKQKIRKLRAVRPDIAISSDFIVGFPGESEADFDKT
                     MKLIEDVGFDQSFSFIYSRRPGTPAADLEDNVADADKHARLARLQAHINGHSLALSRA
                     MVGSIQQVLVTGVSRRDANELTGKTENMRQLNFAGDPRLIGQFVPVVVTEALPNSLRG
                     RLAGGATAG"
     gene            9975..10934
                     /locus_tag="PK818_02105"
     CDS             9975..10934
                     /locus_tag="PK818_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HQY81560.1"
                     /translation="MRAAAAFLMLMSVLAMPAAAGNVYRCDAGDGITSYSNKRVAGGN
                     CKLVGSYKSQRAVKPKARPAPAPVPALAAKEVQPADASRKAAMGGAVPPATVSGSPVA
                     ASLAAATPSPSAPRLIRGQVYAYVGKDGVRHYTSVRPKGVSVASMRTIRYSYMETCFA
                     CGAKPGVDFRSLRLNTAAFNDEIRNAARQHGVEEAIVRAIIHAESAFNPRALSRVGAQ
                     GLMQLMPATARRFGVTDAFDARQNIGGGVQYLAWLLKRFNGNLTLAAAGYNAGEGAVD
                     KYNGVPPYKETRHYVERVAILADRYRSALGPVAAASAGGLSAR"
     gene            11034..11630
                     /gene="petA"
                     /locus_tag="PK818_02110"
     CDS             11034..11630
                     /gene="petA"
                     /locus_tag="PK818_02110"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HQY81561.1"
                     /translation="MANEEVNTGRRRFLTATTAVVGAVGAGFAAVPFIKSWSPSARAR
                     FAGAPVTQDIGALAEGAQMVINWRGQPIFIARRSKAMLEILGGMDALVADPQSGASEQ
                     PKYAQNPTRSIKPDISVLVGVCTHLGCAPEFLPEVKPEPFDPDWKGGYFCPCHKSRYD
                     LAGRVYKSQPAPLNLPVPPYHFESDSVVVIGIDGQGAA"
     gene            11634..12905
                     /locus_tag="PK818_02115"
     CDS             11634..12905
                     /locus_tag="PK818_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007965674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HQY81562.1"
                     /translation="MANIINRSVDTVWNWVNERAPGMMPAYRKHMSEYYAPKNFNIWY
                     IFGVLSIVVLVNQILTGIFLTMHFKPSAAEAFASVEYIMRDVEWGWLIRYMHSTGASL
                     FFVVVYLHMFRGMMYGSYKKPRELVWLLGMVIYLVLMAEAFLGYVLPWGQMSYWGAKV
                     IISLFGAIPVIGVGLTEWIMGDYNPGDATLNRFFALHVIALPLVLLLLVVLHIFALHE
                     VGSNNPDGVEIKKGPKGNRWSPNAPADGIPFHPYYTVKDTFYVGFFLILAAFIIFFAP
                     AFGGWFLEHDNFTEANPLVTPAHIKPVWYFTPYYAMLRVVPSFFGIKLWGVLVMFGAI
                     VMLFLLPWLDRSQVKSYRYRGVLSWAMLMMFVVSFLWLAKIGAGPGTDPTEAIIGRVL
                     TFLYFAFFISMPVWTKLDRTKPVPERVTMHD"
     gene            12898..13641
                     /locus_tag="PK818_02120"
     CDS             12898..13641
                     /locus_tag="PK818_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HQY81563.1"
                     /translation="MTNNLVSRAAVFAAGLLLSASAFAAGGGNLQHAGTDVGDKASLQ
                     RGAALYMNYCAGCHSLKYVRYARIGEDLGLSEEEVQGSLNFTGGKIGDHVISPMSAEM
                     GVAAFGKAPPDLSVIARVRGSDWIFTYLKSFYLDESRPVGWNNTLFANASMPNPLWEL
                     QGLQQPVYGKRTAAGEMPVEGFTVSQPGTQDAAAFDQTVRDITAFLEYAGEPAATKRE
                     GLGVWVILFLAFFTMMAWLLKKEYWRDVH"
     gene            13750..14385
                     /locus_tag="PK818_02125"
     CDS             13750..14385
                     /locus_tag="PK818_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HQY81564.1"
                     /translation="MAVSPRMRNALTLFSSVDDVLCHRVRLVLAAKGVTYDLIPVDPQ
                     NPPEDLIDLNPFHSVPTLVERDLVLYAASVVGEYLDERYPHPPLMPVDPLSRARLRLA
                     MLRIEHEWVPHVQAIQLGNKQQADAGRKRLKELLTAAVPVFKASKFYLNHEMSLADCA
                     MAPIIWRLPSLDIPLPKDGKAIEDYGNRIFRNPGFVRSLTEKEKTLRDLPA"
     gene            14416..14880
                     /locus_tag="PK818_02130"
     CDS             14416..14880
                     /locus_tag="PK818_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016901612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HQY81565.1"
                     /translation="MGDDAAPMSSHRPYLLRALYEWIVDNGMTPHVLVDAGQPGVRVP
                     AHAVKDGRIVLNIAERAVGGLRMDNDALRFSARFGGVSQSVLVPLPAVIAVYARETGH
                     GMALPEDPRGVEAQLQAEPVSASVEDSGAPDDDGPGSPGEPPRRGGHLRIVK"
     gene            complement(15002..15340)
                     /locus_tag="PK818_02135"
     CDS             complement(15002..15340)
                     /locus_tag="PK818_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HQY81566.1"
                     /translation="MPTLLILMIVGVAAFFAFTAARAAAERAAEIGRDACHAAGVQWL
                     DQSVHAIGMRLRRQDSGWLGVERSFRFEYSEDGHDRHVGRVVLLGEKLVGFSGPSRAA
                     QAVVRSFPLQ"
     gene            15404..16165
                     /locus_tag="PK818_02140"
     CDS             15404..16165
                     /locus_tag="PK818_02140"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HQY81567.1"
                     /translation="MMMKLVFPAGDRPQMLLDQGAFRIGSAANADLVLASDGIEPLHC
                     ELQVGAQGVRMRVPEGIRVLVNERPVEGLIALRADDTVGLASTRFRLLDAAADAGRSA
                     SVARSDDAGETNATMVRPVVPKFALRGLSGELFGRSFPLSASLSVGRADDAGLRIPLE
                     SISRLHARLTPAGDEVLVEDMGSANGTWLNGKRITRAQAVHGDEIRFDTQRFQLLVPG
                     QAIHAQAQPARNGLRWQWLAAALVVVVAGAAWLLR"
     gene            complement(16170..17045)
                     /gene="nadC"
                     /locus_tag="PK818_02145"
     CDS             complement(16170..17045)
                     /gene="nadC"
                     /locus_tag="PK818_02145"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HQY81568.1"
                     /translation="MSAGRFAPPPAEVVAADVAAALAEDIGSGDATAGLLEDAPDSAY
                     LLCKEDCVLAGRAWFEACHRALDPGVRFDWCCEEGDHVARGSVIAILQGRNRALVSAE
                     RASLNFLQTLSGTATATARYVAAVRGTGATILDTRKTIPGLRQAQKYAVRVGGGSNHR
                     IGLFDAVMLKENHIRAAGSLTAAVERARALHPRLPLIVEVETLAQLREALDAGCTRIL
                     IDDFDPDTRREAVRIARSAPHDGRIPLEVSGGVDLASVRGIAEDGVDCISIGGLTKHL
                     HAIDFSLKLGPPPAA"
     gene            complement(17042..17311)
                     /locus_tag="PK818_02150"
     CDS             complement(17042..17311)
                     /locus_tag="PK818_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HQY81569.1"
                     /translation="MDRKLLDILVCPATRQPLSILESAGLDALNRAIAAGGVTRGDGS
                     DQSESLREALVTRDRKTVYRIDDGIPVLLADDALATAQVEGFPKA"
     gene            17369..17872
                     /gene="purE"
                     /locus_tag="PK818_02155"
     CDS             17369..17872
                     /gene="purE"
                     /locus_tag="PK818_02155"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HQY81570.1"
                     /translation="MPAAADAPLVGIVMGSRSDWETMQHASAKLEALGVPHEVRVVSA
                     HRTPDVLFEYAASAESRGLRAIIAGAGGAAHLPGMLAAKTVVPVLGVPVQSKALNGMD
                     SLLSIVQMPAGIPVATFAIGTAGAANAALFAAAMLAGAHPAIAGALAAFRARQTAEVA
                     GNDDPRR"
     gene            17886..19025
                     /locus_tag="PK818_02160"
     CDS             17886..19025
                     /locus_tag="PK818_02160"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HQY81571.1"
                     /translation="MTTVGILGGGQLARMMALAGAPLGLRFRVLDNVADACAGQFAPM
                     IVGDYTDQAALAQFAAQVDVATFDFENVPAESAQWLSERVPVFPNPRALAVAQDRLAE
                     KTLFRTLGIPVPGFADIGSRAQLEAAVAELGTPCILKTRRLGYDGKGQFRIKSAADID
                     AAWDALGAQAMTVGLILEGFVHFQRELSVVAVRGRGGEFRAWPLTENWHVDGVLSASL
                     APARVEDALQATALAHARKLAEALDYVGVFALELFCRDGELLANELAPRVHNSGHWTI
                     EGSETSQFQNHLRAVLGLPLGDTRMVGTACMLNWIGTMPDANAVLGEPGGHWHDYGKA
                     PRDGRKVGHATLRADSAEELAMALQRVGVALDREGQVAPVVAALR"
     gene            complement(19093..19671)
                     /locus_tag="PK818_02165"
     CDS             complement(19093..19671)
                     /locus_tag="PK818_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HQY81572.1"
                     /translation="MAIELPALPYDRTALEPHISGETIDFHYGKHHQAYVTNLNNLIA
                     GTEFADMSLEDIVRKSQGGMFNNAAQVWNHSFYWNCLKPNGGGEPAGKLAEAINAAFG
                     SFDRFKEEFGKTAIGTFGSGWAWLVQRADGSLALASTPNAATPLTGEDTPLLTCDVWE
                     HAYYVDYRNARPKYVEAFWNLVNWDFVAGNMK"
     gene            19805..20125
                     /gene="grxD"
                     /locus_tag="PK818_02170"
     CDS             19805..20125
                     /gene="grxD"
                     /locus_tag="PK818_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HQY81573.1"
                     /translation="MSSVEERIRAEIEGHPIVLFMKGTPQFPMCGFSSRSLQALMAAG
                     AQLHTVNVLEEPEIRANLPRYSDWPTFPQLFINGELIGGCDITVELSESGELARIVAD
                     AQKA"
     gene            20122..20850
                     /locus_tag="PK818_02175"
     CDS             20122..20850
                     /locus_tag="PK818_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY81574.1"
                     /translation="MSGASSLEGRVILVAGAAGGLGSAASVACAQAGATVVLLGRKVA
                     PLNRVYDAARAVGPEPVLYPLDLEGASPDDFDELGQRIEADLGRLDGLLHCAAEFKGL
                     TPLQHTDPADFARALHVDLTARWWLTQACLPLLAKSDAGAVVFAIDDPARNGSPAFWG
                     GYGLAQSAQVTLVPMLQAELGEHGPRVSGLRPGPMRTGLRGKAFQPNTDLKAKPASAY
                     AAACVELLSAAGAAQRGRIVDVLA"
     gene            20847..21440
                     /locus_tag="PK818_02180"
     CDS             20847..21440
                     /locus_tag="PK818_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhgN family NAAT transporter"
                     /protein_id="HQY81575.1"
                     /translation="MTIAAAALLLFLILDPLGNIPVFLSLLKGLPPARQRVVIVRELL
                     IALGVLMLFLWTGRFLLELMHLRQESVSIAGGIILFLIGLRMIFPSADGIMGEQPEGE
                     PFIVPMAIPMIAGPSGMAAVMLLGSQEPGRMGAWSLALAIAWAATAIILFSATYLQKW
                     LGTRVLTAIERLMGMVIVAISVQMLLDGIASYLRAGS"
     gene            21658..>22940
                     /locus_tag="PK818_02185"
     CDS             21658..>22940
                     /locus_tag="PK818_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY81576.1"
                     /translation="MTHPNRVRLSKLSLGLMVALAAAPVFAQSTSAGVGGQVVGADGQ
                     PVAGAEVTITHIDSGTVSRATTDAAGRYNARGLRVGGPYTVTINKAGAGSDTEENVYL
                     TLGTANTIDAQLNNDVATLGTVQAVATAGTSVFSATKMGAGTAVDQNTIKALPSINGN
                     IQDFMRLDPRVAFTDRASGSMSAGGLNPRFNKITIDGVSASDTFGLEGNNMPTQRQPV
                     ALEAIEALDINLSNYDVTMSGAAGANVNAVTKSGTNQFHGALYGNYRDGDWFGDYPAR
                     VAGSPLDVTGVPFDEFDEEKTWGFTLGGPIIKDKLFFFANYEKLTQTNITSAGGKSVA
                     SNPLIVTGADFGAAEVAEVQRIAEEVWGFDAGGLIGGNTELEEYAVKFDWNISENHRA
                     NLRYSKLEQVRVRPEASTAGSLSLSSNWFNHEKTV"
BASE COUNT         3435 a         7801 c         8062 g         3642 t
ORIGIN      
        1 ccgtgcatgg ctacgtggtc aagccgtgga actaccagcc gggcaggaag tacccagtgg
       61 cgttcctgat ccatggcggc ccgcagggca gcttcggcga tggctggagc taccgctgga
      121 acccgcagac ctatgcgggg ctgggctatg cggtggtgat gatcgacttc cacggttcca
      181 ccggctatgg ccaggcattc accgattcga tcagccagca ttggggcgac cgcccgctgg
      241 aagacctgca gaagggctgg gcggcggcgc aggcgaagta cgccttcctc gacggcgaca
      301 aggcctgcgc gctgggtgcc agctacggcg gcttcatggt caactggatc gccggcaact
      361 ggccgcagcc gtggaagtgc ctggtctcgc acgatggcgt gttcgaccag cgcgcgatgg
      421 gctacgccac cgaggaactg tggttcaccg aatgggagca gggcggcacc ccgtacgaca
      481 agcctgcgaa ctacgagcgg ttcaacccgg tcaaccacgt caaggactgg aaggtgccga
      541 tgctggtgat ccacggccag ctggactacc ggatcccggt ggaacagggc atcgcggtgt
      601 tcaccgcgct gcagcggcgc gggatcgagt cgaagttcct gtacttcccc gacgagaacc
      661 actgggtgct gaagccgcag aacagcgtgc tctggcacga caccgtcaac ggctggttgc
      721 agcagcacat cgggcagtga tgtcgttccg tccgcacgca tgaacgaagc aagccgcacg
      781 gtcttgatcg gcaacgacgc cgcggtcctc ggactgctgg cgatgatcct cggcttcgtg
      841 ttctggggcg cctcgcgcga gcgcggcttc tggaagaagt tctatgcggt ggtgccggcg
      901 atcctgccct gctacctgct gccggcgatc gccaacagca ccgggctggt cgatggcgcc
      961 gggtccgggc tgtacccgat ggcgcgcgac tacctgctgc cggcgtcgct ggtgctgttc
     1021 tgcgtgtcga tggacctcaa gggcatcgtg cggttggggc cgaaggcgct ggtgatgttc
     1081 ctgaccggta ccgtcggtgt catgctcggt gcgctggtgg cgttcgaggc gatgcggttc
     1141 atccatcccg ccaccgtcgc cggcgagacc tggcgcggca tgaccacgat cgccggcagc
     1201 tggatcggcg gcggcgccaa ccaggcggcg atgaaggaag tgttcgcggt ggatgccacc
     1261 gtgttcggcc agttcgccgc ggtcgacgtg atcgtggcct acctgtggat ggcggtgctg
     1321 ctgttcatgg cctcgcgcag cgcggcgatc gatcgctgga accgggccga cacctccgcg
     1381 atcgacgacc tgcgccggcg catcagcgac taccaggccc agcacgcgcg ggtggcgtcg
     1441 accaccgacc tgatgctgat cttcgcgctg gcgatcggga tcaccgggct ggcgcacctg
     1501 ctgaccgatc cgctggtggc ctgggtcagg gcggcaccgg ccgcatggcg cctgcaggac
     1561 tacagcctgg atgcgcgctt cttctggatc gtggtcttcg cgaccacctt cggcgtgctg
     1621 ctgagcctga cccgcgcgcg ccagctggaa ggcgcaggcg cttccagcgt cggctcggtg
     1681 ttcctgtaca tcctgatcgc caccatcggc atgcagatgg acctgaaggc gctggcggac
     1741 aagcccttgc tgctgctgct cggcctgatc tggatcggcg tgcatggcgg cctgatgctg
     1801 ctggtggcac gcctgatccg tgcgccgttg ttcttcatgg cggtcgggtc gcaggccaac
     1861 atcggcggcg ctgcgtcggc gccggtggtg gccaacgcgt tccacccggc gctggctccg
     1921 gtcggggtgc tgctggcggt gatgggatac gccctgggca cctattgcgc ctacctgacc
     1981 gggctggcct tgcgcgcgct ggcggcgtga ggcggcggcc gcgctacagc cagcggatct
     2041 tgcgcaggta gcggtacagc agcacgcagg acagggcaac gccgccgatc agggccgcgt
     2101 agctgtagcg cccgtgcagc tccggcatgt gccggaagtt catgccgtac cagctggtga
     2161 ccagggtggg cgcggccagc aggccggccc agccggccag cttcttgacg atctcgccct
     2221 gcgccacggt gaccagcgac aggttcacgt tcatcgccgc cgacaccatc tcgcgcaggg
     2281 tgtcgatgct gtcgccgacg cgggccacgt ggtcgagcac gtcgcggaaa tacggcttga
     2341 cctcgtccgg gatcgccacc accggggtgc ggatcagctg cgacaggatg tcctgcatcg
     2401 gcgccaccgc catccgcatc cgggtcagtt ccttgcgcag gtcgtacagg cgccggatcg
     2461 tgccgcgctt gtaggtttcg gcgaacacgt cctgctccag catgtcgagc tcgtcctcga
     2521 agtcggacac gatcggctgg tagttgtcga cgatgaagtc cagcaccgcg tgcacggcga
     2581 cgctgggccc gtgcgcgagc gccgcggggt cccgctccag gcggtcgcgc acgatcgaga
     2641 aggtgagcga tgcgccgtgg cgcacggtga ccaggaagcg cgggcccacg aacaggtggg
     2701 tctcgccgaa gcggacatgc gcgtcgatcc gctgcgcggt atgcacggcg atgaacaggc
     2761 tgttgccgaa cgcctccagc ttcggccgct ggtgcgcgtg ctgggcgtcc tcgatggcga
     2821 ggtcgtgcag cccgaactcc tcctgcatct tctccagcag gccttcctcc ggctcgtaca
     2881 ggccgaccca gacgaagctg ccgtcgtcca ccgccagcac gtcgctgacc gcgtccaggc
     2941 tgatgtcgcg gcggtggccg tcgcggtcgt aggccgcgca gttcaccacg caggcgggca
     3001 gggcggtggc gggcgtgttc atcgccgcat cctgcatcgc gggcgcgggt ccatcaaggc
     3061 ttgcgccggc cgcggcacca cgcgaacgcc gcgtgcaccg gcagcaacag cgcgatccag
     3121 gggccgttgc gttgcggcat tgcctgcagc cacagcggca gcagcgccac ggcggcgagc
     3181 gcggcaaccg caaccagcaa cgatcggaag cggccggaac cggcgtgccc gcgcagcaac
     3241 gcccatccac ccggcaacag cagcaggcat agcgggttga acagcagcag gttgcggttc
     3301 gcccacagcg cgcggtgcgc ggtgaatccc cagcccagca gcagcaccag cccgaccgcg
     3361 ccgcacagca gccagaagcc cagccccacg ccgccgagca ggcgcggcgc gcggcgcgcg
     3421 caggccagga tcgatgccgc cagcgccagc cccagggcca gccagggcca tgcgtgacgg
     3481 gggcgttcgt cgggttccgc cggttgccgt tgcggcagca gttgctgttc ggcgacgacc
     3541 agcgggcggc cgtcgggcag cttcgcgtcg cgcacgtcgt cggccagccg gcgcggcagg
     3601 aatgcctgct cccagcgcga cagcgggcgg tcgccgtagg gccccaggcc gacgtcgaac
     3661 agcagccaca tccacggttc gggcgaggcc aaccgcaccg attcgctgcg atagctgtcg
     3721 ccgctcgaac gcggttccat ctgccggcgc aggttgccgc cgagcgcgcg gtcgagcgca
     3781 tcgcgcacgc gggtggcgca attgtcggtg aagtagtcgt agcggtagcg cgcgttttcc
     3841 ggcttcgcgt tctcggccag gtcggcggcg agctggcgcg cctgcgcggg ctccagcgcc
     3901 agccactgca gcgacgcacc gcgcccgacc tcgcggtagt actgcatgtc gtcgtccagc
     3961 ggcaacgcga ccagcctgta ctgcatctcg ccacgcacga agcggccgat gaagccgtct
     4021 tccgccaggt cgaagaaacc gaagttgtac gagatcggtt cgccgcgcgc ggggtcgtcg
     4081 accacgatcg cgtcgtggcc gaagcgttcc cagaacacgt cgccgggccc catggtgacc
     4141 accccgatcc gcggtggctg cccagtgtcc gcggcttgcg catgcacccg gcccgcggac
     4201 aacgcgagcg ccagcaacag ggcgaagcgc agcgcctcag tgatccgcat gcacgcccac
     4261 gtgcagcgca tgcaggcggc gtgcgtcggc gcgggcgacg cggaacacga agcggcccag
     4321 ggtcagttcc tcgccggcct ccggcaggtg gccgatggca ttggtgacca ggccgccgat
     4381 ggtgtcgtac tcgtcgtcgt cgaagtcggc gccgaagcgt tcgttgaagt cggcgatcgg
     4441 ggtcagcgca tcgaccacga actggccgtc cgcctgcgcc gcgatcaggg tcgatccgtc
     4501 ctcggcctcg tcgtgctcgt cgtcgatgtc gccgacgatc tgctccagca cgtcctcgat
     4561 ggtgaccagg ccggcgacgc cgccgtattc gtcgatgacg atggccatgt ggttgcgcga
     4621 ctggcggaac tcgcgcagca gcacgttgag cttcttcgat tccgggatca gcaccgccgg
     4681 gcgcagcagt tcgcggatcg tgcccgggcc gttgtcggcg accacgccgc gcagcaggtc
     4741 cttggccagc aggatgccga ggatctcgtc cttgtcctcg ccgtggaccg ggaaccgcga
     4801 gtggccggat tccaccactt ccttcatcag gtccaggaag cgcgcgtcgg ccggcagcgc
     4861 caccatctgc gcgcgcggca ccatcacgtc gcccacgctc atgtcggaaa ccgcgatcgc
     4921 gccttccatc atgcgcaggg tgtcggcggc gatcaggccg tcggcctgca cgtcgcgcag
     4981 cagttcgacc aggtcctcgc ggctggtcgg ctcgccggac agggcggcgc tgatgcggtc
     5041 cagccaggag cggcgcggct tgtcggcgga acgctccgcg gagtcggcgg tttcgtgacg
     5101 actgtcgtcc tcggacatgg gctggcaggg gccttgggca aacgggaggg aagtctagcg
     5161 aactcgggtg gcgcggcggt gaacccggtc aacgctcgat atacgggtcc ggcaggccca
     5221 gtccggccag gatgccgcgc tccagttgct ccatgcattc ggcctcgcgc ggatcctcgt
     5281 ggtcccagcc gagcaggtgc agggtgccgt gcacggtcag gtgcgcgtag tgcgccgcca
     5341 gcggcttgcc ctgcgcggcg gcctccttcg cgatcaccgg cgcgcacagc accaggtcgc
     5401 ccagcagcgg cagcctgacg ccctccggca gcccctcggg caattccgcg gggaagctga
     5461 gcacgttggt ggcgtagtcc ttgccgcggt agtggtggtt gaacgcgcgg ccttccttct
     5521 cgtccaccac gcggatcgcg aggtcggcct cgcggatgcg gccggccagc gccgccgcga
     5581 cccacttgcg gaagctcacc gcggcaggga tcccggcgcg cggcaccgcg tagccgacgg
     5641 cgacatcgag gcggacggga cccttggtca tgcccgcgat cttacgccga accggcctgt
     5701 ccatccttcg cgtcgcgcgc ctcgtaggcg ttgacgatgc gcgccaccag cgggtggcgg
     5761 accacgtcgc gcgattcgaa gaaggtgaag tgcacgccgt cgacgccgcg cagcacgtcc
     5821 accgcgtcct tcagcccgga cttctggtgc ttgggcaggt cgatctgggt catgtcgccg
     5881 gtgatcaccg cggtgctgcc gaagccgagc cgggtcagga acatcttcat ctgctcgatg
     5941 ctggtgttct gcgcttcgtc caggatcacg aaggcgtcgt tgagggtgcg cccgcgcatg
     6001 taggcgagcg gggcgatctc gatgacgttc ttctccagca gccgcgccac cttttccacc
     6061 ccgagcatct cgtacagcgc gtcgtacagg gggcgcaggt acgggtccac cttctgggtc
     6121 aggtcgccgg gcaggaagcc gagcttctcg ccggcctcga ccgccgggcg caccaggatc
     6181 agccgctgca cgcgcgactg gttgagcgcg tccaccgcca tcgccaccgc gaggaaggtc
     6241 ttgccggtgc cggccgggcc gatgccgaag ttgatgtcgt ggctggcgat cgcgtgcagg
     6301 tagttctgct ggttgggccc gcgcccgcgc acgttgccgc gcttgacccg cacggagatc
     6361 tcctgcggca cgtggtccgg ggcttgcacc gcggggatgt catcgcgggc gtacgcgccc
     6421 agccgcaggt ggatggcgtg ctcgtccagg gtttccgacg cggcgtcctg gtacagctcg
     6481 cgcaacaggc gctcaccacg cgccaccgcg tcgccgtcgc ggccactgac gcggaacacg
     6541 ctgccgcggt tggcgatctc cacgcccagc cgcagttcga tcatccgcag gtgcgcatcg
     6601 aacgggccgg acaggttggc caggcgttcg aggtcatcgg gttcgagggt gaaatcgcgt
     6661 tgggtctgca tgccgggaag ggtagcgcgc cgtccggggg catgccattc gcggcttgcc
     6721 tctcgccacg gcttgcgcca atgtccgggt tccgcgacgc gaggggatcc gggatgcgca
     6781 agcttgcatg ggtggtgtcg gcgattttgc tggcggcggc ggggtcgccg gctgcgcagg
     6841 acggcgtgac cctggtgacc gcgtcgcgca tccacacgat ggataccgcg cggccgatgg
     6901 caacggcgat ggcgtacggc gccgatggcc ggatcctcgc ggtcggccag gccgatgccc
     6961 tggcgaagca gtacccgcgg gcgcggcgcg tggacgtggg caacgcgacg gtggtgccgg
     7021 ggctgatcga tgcgcatgcg cacatcggcg ggctggggct ggcgatgctc agcgcggacc
     7081 tggtcggcac ccgcgacaag gccgggatcc tgcagcgcct gcgcgtgcac gagcagaccc
     7141 tcaagcccgg cgagtggctg gtcggcggtg gctgggacca gaacgactgg ccgcaacaac
     7201 gcttcccgaa tgccgcagac ctggatgccg cgttccccga tcggccggtg tggctgtcgc
     7261 gggtggatgg ccatgcgggt tgggcgaaca gcgcggcgat ccgcgcggtg cgccgcgacc
     7321 tgtccggcga ctggcagccg gatggcgggc gcatcgaccg cgatgccgcc ggcaagccga
     7381 ccggcctgtt catcgacaac gcgatgttgc tggtcgaaca ggcgcgcccg gcgatgtcgg
     7441 aggcgaccgc cgagcacgcg ctcgagatcg cgatgcgtgc ggccgccgag cagggcctga
     7501 ccggcgtcca cgatgccggc atcaccctgg ccgagctggg ccgctaccag cgcctggccg
     7561 atcgcgggcg gatgccgctg cggatcagcg cgatggccga cggcgacagc gcggcgctgg
     7621 agtcgctatg ccgcaacggg ctgtaccggc atccgtcggg ccggctgcag atgcgcgcgg
     7681 tgaagctgta cgcggacggc gcgctcggca gccgtggcgc ggcgatgctg gaggactaca
     7741 gcgacgacca cggcaaccgt ggcctgatgg tcatgtcgcc ggcgcaactg gcggcggcca
     7801 ccgccaaggc caagcgctgc ggcgtgcagg tggcgaccca cgcgatcggc gaccgcggca
     7861 accgcgaggc gctggacgcg tacgccacgg cgctgggcac ggatgcggcc agcgaccatc
     7921 gctggcgcat cgagcacgcg cagatcctgt ccgcgcagga cctgccgcgg ctcgcggggt
     7981 tgcgggtgat cgcctcgatg cagcccaccc acgccaccag cgacatgccg tgggcggagg
     8041 accgggtcgg cgcgcagcgc atcgccggcg cctacgcctg gcggcaactg cgcgacagcg
     8101 gtgcacggct cgcgctgggg tcggatttcc cggtggagcg ggtcgacccg cggctgggcc
     8161 tgcaggccgc gaccacccgc gccgatggcg agggcaagcc ggtgggcggc tggttccccg
     8221 gcgaaaagct gaccgcgttc gaggcgctgc gcgggttcac cctggatgcc gcccacgccg
     8281 gcttcgccga agccgaggtg ggcagcctgg tcgccggcaa gcgcgccgat ttcgtggtgc
     8341 tggcggcgga tccgctggca tcgccggagg cgcggctgaa ggagatcgcg gtgctggcta
     8401 cctacgtcga cggcaagccg gtgttcgagg ccgcctccgg gcgttgagca ggtgggcggc
     8461 ggtcagcccg cggtcgcccc gccggccagc cgcccgcgca gcgagttcgg caacgcctcg
     8521 gtgaccacca ccgggacgaa ctggccgatc aggcgcggat cgcccgcgaa gttcagctgg
     8581 cgcatgttct cggtcttgcc ggtgagttcg ttcgcgtccc tgcgggaaac gccggtgacc
     8641 agcacctgct ggatgctgcc gaccatcgcc ctggagagcg caaggctgtg gccgttgatg
     8701 tgcgcctgca ggcgcgccag ccgcgcatgc ttgtcggcat cggcgacatt gtcctcgagg
     8761 tccgcggccg gggtccctgg gcggcgcgag tagatgaagc tgaagctctg gtcgaagccg
     8821 acgtcttcga tcagcttcat ggtcttgtcg aaatccgcct cggactcgcc cgggaagccg
     8881 acgatgaaat ccgaggaaat cgcgatgtcc ggccgcaccg cgcgcagctt gcggatcttc
     8941 tgcttgaact ccagcgcggt gtagccgcgc ttcatcgcgg ccaggatgcg gtcgctgccg
     9001 gactgcaccg gcaggtgcag gtagttggcc agcttcggta cgtcgcggta ggcctccacc
     9061 agcgcgtccg agaactccag cgggtgcgag gtggtgaacc ggatccggcc gatgccctcg
     9121 atctcggcga tcgcgcggat cagcaggccg aggtccgcga attcgccttc gccatacggc
     9181 ccgcgatagg cgttcacgtt ctggccgagc aggttgacct cgcgcacgcc ctgcgcggcc
     9241 agttgcgcca cttcgaccag cacgtcctcg aacgggcggc tgacttcctc gccgcgggtg
     9301 taggggacca cgcagaaact gcagtacttg ctgcaaccct ccatgatcga gacgaacgcg
     9361 ctcggaccgt ctgcgcgcgg ctccggcagg cggtcgaact tctcgatttc ggggaagctg
     9421 atgtcgacct gcggcagccc ggaggcgcgg cggtcccgga tcagttccgg aagccggtgc
     9481 agggtctgcg ggccgaacac caggtccacg tagggcgcgc gcttgacgat ggcggcgccc
     9541 tcctgcgagg ccacgcagcc gccgaccccg atcagcaccg gacgtccgtc cttcttcagc
     9601 gatttccagc ggcccagctg gctgaacacc ttttcctggg ctttttcgcg aatcgagcag
     9661 gtattgacca ggataacgtc ggcttcttcg gcgatcgtgg tcagttccat gccgtccgcg
     9721 gcggccagga cgtcggccat cttcgccgag tcgtactcgt tcatctggca gccgtgggtc
     9781 tggatgtaca gcttgccgcg ggcgccggtg gcgggcgcgg cttcggttgt ttcggcgctc
     9841 atgctctctc tccggggcgc ggcgggtaat ccgggccggt cgtgcagtct agcggcgatg
     9901 gcccatccac ggggcgtcct gcgttcacaa tcaatgactt gtaatgaccc ggtgaagagg
     9961 ggagactgcc ggcgatgagg gccgccgccg cattcctgat gttgatgtcc gtgctggcga
    10021 tgccggccgc ggccggcaac gtgtaccgct gcgacgccgg cgacggcatc acctcctatt
    10081 ccaacaagcg ggttgccggc ggcaactgca agctggtcgg cagctacaaa tcgcaacggg
    10141 cagtgaagcc caaggcgcgg ccggcaccgg cgcccgtacc ggcgctggcg gcgaaagagg
    10201 tgcagccggc ggacgcctcg cgcaaggcgg cgatgggtgg ggcagtcccg cccgcaaccg
    10261 tttccggctc accggtggcg gccagccttg ccgctgccac gccgagccca agcgccccgc
    10321 ggctgatccg cggccaggtc tacgcctacg tcggcaagga cggcgtgcgc cattacacca
    10381 gcgtgcgccc gaaaggcgtt tccgtggcgt cgatgcggac catccgctac agctacatgg
    10441 aaacctgctt cgcctgcggc gccaagcccg gtgtcgactt ccgcagcctg cgcctgaaca
    10501 ccgccgcctt caacgacgag atccgcaacg ccgcgcgcca gcacggggtc gaggaggcga
    10561 tcgtccgcgc catcatccac gccgagtcgg cgttcaatcc gcgggcgttg tcgcgggtcg
    10621 gggcgcaggg gctgatgcag ctgatgcccg ccaccgcccg ccggttcggg gtgaccgatg
    10681 cattcgatgc ccgccagaac atcggtggcg gggtccagta cctggcctgg ctgctcaagc
    10741 gcttcaacgg caacctgacc ctggccgcgg ccggttacaa cgccggcgag ggcgcggtgg
    10801 acaagtacaa cggcgtcccc ccgtacaagg agacccgcca ttacgtggag cgggtcgcga
    10861 tcctggccga ccgctaccgc agcgccctgg gtccggttgc ggccgcctcc gccggcggat
    10921 tgtcggctcg ttaagcgttc cgttacactt ccccgtcttt tgccgcaggc tggcgcgcgc
    10981 aagcgccagg cgagcctgcg caaccgttgt ttggattttt ttcggagtgc gggatggcca
    11041 acgaagaggt caacaccggc cggcgcaggt tcctcaccgc gaccacggca gtggtcggtg
    11101 cggtgggagc cgggttcgcc gcggtgcctt tcatcaagtc ctggagcccc agtgcgcggg
    11161 cccgtttcgc cggtgcgccg gtgacccagg acatcggcgc gctcgccgag ggtgcgcaga
    11221 tggtgatcaa ctggcgcggc cagccgatct tcatcgcccg ccgctccaag gcgatgctcg
    11281 agatcctggg gggcatggac gcgctggtcg cggacccgca gtcgggtgcc tccgagcagc
    11341 cgaagtacgc gcagaacccg acccgctcga tcaagcccga catctcggtg ctggtcggcg
    11401 tgtgcaccca cctcgggtgc gccccggaat tcctgcccga ggtcaagccc gagccgttcg
    11461 acccggactg gaagggcggc tacttctgcc cctgccacaa gtcgcgctac gacctggccg
    11521 ggcgcgtgta caagtcgcag ccggcgccgt tgaacctgcc ggtgccgccg taccacttcg
    11581 agagcgacag cgtggtcgtg atcggcatcg acggccaggg ggcggcataa gccatggcga
    11641 acatcatcaa ccgcagcgtc gacaccgtct ggaactgggt caacgagcgc gcgcccggca
    11701 tgatgccggc gtaccgcaag cacatgagcg agtactacgc gccgaagaac ttcaacatct
    11761 ggtacatctt cggggtgctg tcgatcgtgg tgctggtcaa ccagatcctc accgggatct
    11821 tcctgaccat gcacttcaag ccgagcgcgg cggaagcgtt cgcctcggtc gaatacatca
    11881 tgcgcgacgt cgagtggggc tggctgatcc gctacatgca cagcaccggc gcctcgctgt
    11941 tcttcgtcgt cgtctacctg cacatgttcc gcgggatgat gtacggctcc tacaagaagc
    12001 cgcgcgaact ggtgtggctg ctcggcatgg tcatctacct ggtgctgatg gccgaggcgt
    12061 tcctcggcta cgtgctgccg tggggccaga tgtcctactg gggcgcgaag gtgatcatct
    12121 cgctgttcgg cgcgatcccg gtgatcgggg ttggcctgac cgaatggatc atgggcgact
    12181 acaaccccgg cgacgccacc ctcaaccgct tcttcgcgct gcacgtgatc gcgctgccgc
    12241 tggtgctgct gctgctggtg gtgctgcaca tcttcgcgct gcacgaggtc ggctccaaca
    12301 acccggacgg cgtggagatc aagaaggggc cgaagggcaa ccgctggtcg ccgaacgcac
    12361 cggccgacgg catcccgttc catccttact acacggtgaa ggacacgttc tacgtcggct
    12421 tcttcctgat cctggccgcg ttcatcatct tcttcgcccc cgcgttcggc ggctggttcc
    12481 tcgagcacga caacttcacc gaggccaacc cgctggtgac ccccgcgcac atcaagccgg
    12541 tgtggtactt caccccgtac tacgcgatgt tgcgggtggt gccgtcgttc ttcgggatca
    12601 agctgtgggg cgtgctggtg atgttcggcg cgatcgtgat gctgttcctg ctgccgtggc
    12661 tggaccgttc gcaggtcaag tcctaccgct accgcggcgt gctcagctgg gcgatgctga
    12721 tgatgttcgt ggtcagcttc ctgtggctgg ccaagatcgg cgccggcccc ggcaccgacc
    12781 cgaccgaggc catcatcggc cgggtgctga ccttccttta cttcgccttc ttcatcagca
    12841 tgccggtgtg gaccaagctc gacaggacca agccggttcc ggaacgggtg acgatgcatg
    12901 actaacaacc ttgtctcccg cgccgcggtc ttcgccgccg gcctgctgct ctccgccagc
    12961 gcgttcgccg ccggcggtgg caacctccag catgccggca ccgacgtcgg cgacaaggcc
    13021 tcgctgcagc gcggcgctgc gctgtacatg aactactgcg ccggctgcca ctcgctgaag
    13081 tacgtgcgct atgcccgcat cggcgaggat ctcggcctca gcgaggaaga agtgcagggc
    13141 agcctgaact tcaccggcgg caagatcggc gaccacgtga tctcgccgat gagcgccgaa
    13201 atgggggtcg cggcgttcgg caaggcgccg ccggacctgt cggtgatcgc gcgcgtgcgc
    13261 ggcagcgact ggatcttcac ctacctgaag tccttctacc tggacgagtc gcgcccggtc
    13321 ggctggaaca acaccctgtt cgccaacgcg tcgatgccga atccgctgtg ggagctgcag
    13381 ggcctgcagc agccggtcta cggcaagcga accgcggccg gcgaaatgcc ggtggagggc
    13441 ttcaccgtca gccagcccgg cacccaggat gccgcggcgt tcgaccagac cgtgcgcgac
    13501 atcaccgcgt tcctggaata cgccggcgag ccggcggcga ccaagcgcga gggcctcggc
    13561 gtctgggtga tcctgttcct ggcgttcttc acgatgatgg cgtggttgct gaagaaggaa
    13621 tactggcggg acgttcactg aagccgcggc gttgcgcgca gcaccgggaa cgacgcttgg
    13681 cggccgtccg catcgtgcgg gcggcgcgtg tctggccggc atcgcccggc cgcgggggtg
    13741 gttctgatga tggctgtgag tccgcgcatg cgtaatgccc tgaccttgtt ctcctcggtc
    13801 gacgacgtcc tctgccaccg cgtccgcctg gtgctggcgg ccaagggcgt cacctacgac
    13861 ctgatcccgg tcgatccgca gaacccgccg gaagacctga tcgacctcaa cccgttccac
    13921 tccgtcccca ccctggtcga gcgcgacctg gtcctgtacg ccgccagcgt ggtcggcgaa
    13981 tacctggacg aacgctatcc gcacccgccg ctgatgccgg ttgatccgct ttcgcgcgcg
    14041 cgcctgcggc tggcgatgct gcgcatcgaa cacgaatggg tgccgcacgt gcaggcgatc
    14101 cagctcggca acaagcagca ggcggacgcc gggcgcaagc gcctgaagga actgctgacc
    14161 gccgcggtgc cggtgttcaa ggccagcaag ttctacctca accacgagat gagcctggcc
    14221 gattgcgcga tggcaccgat catctggcgg ctgccgtcgc tcgacatccc gttgccgaag
    14281 gacggcaagg cgatcgagga ctacggcaac cggatcttcc gcaatcccgg cttcgtgcgc
    14341 agcctcacgg agaaggaaaa gaccctgcgc gacctgccgg catgacccgg ctcgcgccga
    14401 tcacgggttg ctgagatggg cgacgatgcc gcgcccatga gcagccaccg cccgtacctg
    14461 ttgcgggcgc tgtacgagtg gatcgtcgac aacggcatga ccccgcacgt gctggtcgat
    14521 gccgggcaac ccggcgtgcg cgtgcccgcg catgcggtga aggacggccg catcgtgctc
    14581 aacatcgcgg aacgcgcggt cggcggcctg cgcatggaca acgatgcgct caggttctcc
    14641 gcgcgtttcg gcggcgtcag ccaatcggtg ctggtgccgc tgccggcggt gatcgcggtc
    14701 tacgcacgcg aaaccgggca cggcatggca ttgccggagg acccgcgtgg tgtcgaggcg
    14761 cagttgcagg ccgaacccgt cagcgcgtcg gtggaggata gcggcgcgcc tgacgacgac
    14821 ggtcccggca gcccgggcga gccgcccagg cgcggcggcc acctgcgcat cgtcaagtga
    14881 cgccagcggc ctgcgcagcg gcgcagccgc ggccccgatg gtccaccgca ggcacggatc
    14941 ccggcccagc gggatgcgac ggccgcgcgc accctcgtcc acatccgcgc gcgccgcggc
    15001 ctcactgcaa cgggaagctg cgcaccaccg cctgcgcggc ccggctcggc ccgctgaaac
    15061 cgaccagctt ctccccgagc agcacgaccc ggccgacgtg gcggtcgtgc ccgtcttcgg
    15121 aatattcgaa gcggaagctg cgttcgaccc cgagccagcc gctgtcctgc cggcgcaggc
    15181 gcatgccgat cgcatgcacc gactggtcca gccattgcac gccggccgcg tggcaggcat
    15241 cgcggccgat ctcggcggca cgctcggcgg cggcgcgcgc ggcggtgaag gcgaagaacg
    15301 ccgcgacacc gacgatcatt agaatgagca acgtgggcat gccgccaatg tggcgatgcc
    15361 gggcgtcgat cgcaagcacc atgactacgg agcaggactt cgcatgatga tgaaactggt
    15421 gttcccggcc ggcgaccggc cgcagatgct gctcgaccag ggcgccttcc ggatcggttc
    15481 ggccgcgaac gcggacctgg tgctggcaag cgacggcatc gaacccttgc actgcgaact
    15541 gcaggtcggc gcgcagggcg tgcgcatgcg ggtgccagag ggcatccggg tgctggtcaa
    15601 cgagcgcccg gtcgagggcc tgatcgcgtt gcgcgcggac gataccgtgg gcctggcctc
    15661 cacccgtttc cgcctgctcg atgccgccgc cgacgccggg cgttcggcct cggtcgcgcg
    15721 cagcgacgat gccggcgaga ccaacgccac catggtgcgc ccggtggtgc cgaaattcgc
    15781 gctgcgcggg ctttccggcg aactgttcgg ccgcagtttc ccgctgagcg ccagcctcag
    15841 cgtcggtcgc gccgacgacg cgggcctgcg gatcccgctc gaaagcatct cgcgcctgca
    15901 tgcgcggctg accccggccg gcgacgaggt gctggtcgag gacatgggct cggccaacgg
    15961 cacctggctc aacggcaagc gcatcacccg cgcgcaggcg gtgcatggcg acgagatccg
    16021 cttcgacacc cagcgcttcc agctgctggt gccgggccag gcgatccacg cacaggcgca
    16081 gcccgcacgc aatgggctgc gctggcagtg gctggcggcg gcgctggtgg tggtggtcgc
    16141 cggcgcggcc tggctgctgc gctagccgct caggccgccg gcggcgggcc cagcttcagc
    16201 gagaaatcga tcgcatgcag gtgcttggtc aggccgccga tcgagatgca gtccaccccg
    16261 tcctcggcga tgccgcgcac gctggcgagg tcgacgccgc cggagacttc cagcgggatg
    16321 cgcccgtcat gcggcgcgga gcgggcgatg cgcaccgcct cgcgccgcgt gtccgggtcg
    16381 aagtcgtcga tcaggatgcg ggtgcagccc gcgtccagcg cctcgcgcag ctgggccagg
    16441 gtttccactt caacgatcaa tggcagccgc ggatgcagcg cgcgcgcgcg ttcgaccgca
    16501 gcggtcagcg agccagccgc gcggatgtgg ttttccttga gcatcaccgc atcgaacagg
    16561 ccgatgcggt ggttgctgcc gccgcccacg cgcaccgcgt atttctgcgc ctggcgcagg
    16621 ccgggaatgg tcttgcgggt atccaggatg gtcgcgccgg tgccgcgcac ggcggcgacg
    16681 tagcgcgcgg tggcggtcgc ggtgccgctc agggtctgca ggaaattcag tgaagcgcgc
    16741 tccgcgctga ccagcgcgcg gttgcggccc tgcaggatgg cgatcacgct gccgcgggcg
    16801 acgtggtcgc cttcctcgca acaccagtcg aagcgcacgc ccgggtccag cgcgcgatgg
    16861 caggcctcga accacgcgcg cccggccagc acgcagtctt ccttgcacaa caggtaggcg
    16921 ctgtccggcg catcctcgag caacccggcg gtggcatcgc cgctgccgat gtcctcggcc
    16981 agcgcggcgg cgacgtcggc ggccaccacc tccgccggcg gcggcgcgaa ccggcctgcg
    17041 ctcatgcctt cgggaagcct tcgacctgcg cggtggcgag ggcgtcgtcg gccagcagca
    17101 ccgggatgcc gtcgtcgatc cggtacacgg tcttgcggtc gcgggtgacc agtgcttcgc
    17161 gcagggactc ggactggtcg ctgccgtcgc cgcgggtaac gccgccggcg gcgatcgcgc
    17221 ggttcagcgc gtccagcccg gcgctttcga ggatcgagag cggttgccgg gtggcggggc
    17281 agaccaggat gtccagcagt ttgcgatcca tgtcggtgct gcggaatggc gggatcgcta
    17341 gaatagcctt ttgacggcgg acaccgacat gccagcagcc gcggatgcac ccctggtcgg
    17401 gatcgtgatg ggctcgcgct ccgactggga gacgatgcag cacgccagcg caaaactgga
    17461 ggcgctcggg gtgccgcacg aagtgcgcgt ggtttccgcg catcgcaccc cggacgtgct
    17521 gttcgagtac gccgcgtctg ccgagtcgcg gggactgcgc gcgatcatcg ccggcgccgg
    17581 cggagccgcc cacctgccgg gcatgctcgc cgccaagacc gtggtgccgg tgctgggcgt
    17641 gccggtgcag agcaaggcgc tgaacgggat ggattcgctg ctgtcgatcg tgcagatgcc
    17701 ggccgggatc ccggtcgcga ccttcgcgat cggcacggcg ggcgccgcca acgcggcgct
    17761 gttcgcggcg gcgatgctgg ccggcgcgca tccggcgatc gccggcgcgc tggccgcgtt
    17821 ccgcgccagg cagaccgccg aggtcgccgg caacgacgat ccgcgccggt gaccggggcg
    17881 cggcgatgac caccgtcggc atccttggcg gcggccaact ggcccggatg atggcgctcg
    17941 ccggtgcccc gctgggcctg cgcttccggg tgctggacaa cgtcgccgac gcctgcgccg
    18001 gccagttcgc gccgatgatc gtgggcgact acacggacca ggccgcgctt gcgcaattcg
    18061 cggcgcaggt ggacgtggcg accttcgatt tcgagaacgt gccggccgaa tccgcgcagt
    18121 ggctgagcga gcgggtgccg gtgttcccga acccgcgtgc gctggcggtg gcgcaggacc
    18181 ggctggcgga gaagaccctg ttccgcacgc tcgggatccc ggtgcccggc ttcgccgaca
    18241 tcggctcgcg cgcgcagctg gaggccgcgg tcgcggagct cggcacgccg tgcatcctga
    18301 agacgcggcg gctgggctac gacggcaagg gccagttccg gatcaagtcg gcggcggaca
    18361 tcgacgccgc ctgggacgcg ctgggcgcgc aggcgatgac ggtgggcctg atcctggagg
    18421 gcttcgtgca tttccagcgg gaactgtcgg tggtcgcggt gcgcgggcgg ggcggcgagt
    18481 tccgcgcctg gccgctgacc gagaactggc atgtcgatgg cgtgctctcc gccagcctgg
    18541 cgccggcgcg cgtcgaagac gccttgcagg ccaccgcgct ggcgcatgcg cgcaagctgg
    18601 ccgaggcgct ggactacgtc ggcgtgttcg cgctggagct gttctgcagg gacggcgagc
    18661 tgctggccaa cgaactggcg ccgcgcgtgc acaactcggg gcactggacc atcgagggca
    18721 gcgagacctc gcagttccag aaccacctgc gtgcggtgct cgggctgccg ctgggcgata
    18781 cgcggatggt cggcacggcc tgcatgctca actggatcgg cacgatgccg gatgcgaatg
    18841 ccgtgctcgg cgaacccggc ggccactggc acgactacgg caaggcgccg cgggacggac
    18901 gcaaggtcgg gcatgccacg ctgcgcgcgg attcggcgga agagctcgcg atggcattgc
    18961 aacgggtcgg cgtggcgctg gatcgcgagg ggcaggtggc gccggtggtc gctgcgctgc
    19021 gctgatcgaa ccgatgcaac gaaaaacggc cggatcgctc cggccgtttc catgtccgcg
    19081 atgcgcggcg gcttacttca tgttgccggc gacgaaatcc cagttgacca ggttccagaa
    19141 cgcctcgaca tacttcgggc gggcattgcg gtagtccacg tagtaggcgt gttcccagac
    19201 gtcgcaggtc agcagcgggg tgtcctcgcc ggtcagcggg gtggcggcgt tcggggtgct
    19261 ggccagcgcc agcgaaccgt cggcgcgctg caccagccag gcccagccgg agccgaaggt
    19321 gccgatcgcg gtcttgccga actcttcctt gaacctgtcg aagctgccga acgcggcgtt
    19381 gatcgcctcg gccagcttgc cggcgggttc gccgccgcca ttgggcttca ggcagttcca
    19441 gtagaagctg tggttccaca cctgcgcggc gttgttgaac atgccgccct gcgacttgcg
    19501 gacgatgtct tccagcgaca tgtcggcgaa ctcggtgccg gcgatcaggt tgttgaggtt
    19561 ggtcacgtag gcctggtggt gcttgccata gtggaagtcg atggtttcgc cggagatgtg
    19621 cggctccagg gcggtgcggt cgtagggcag ggcgggcagt tcgatggcca cggcgggctc
    19681 ctggtcaggg cggggaaggg tgcggccgga gccgcgcgaa gacttacaat gcgcgattct
    19741 atccccacgc ccgttgccgc actgtcgccc gcggcgggtc gcaacgttgc aggagcaaga
    19801 cagcatgtcc tccgtcgaag agcgcatccg ggccgagatc gaaggccacc ccatcgtcct
    19861 gttcatgaag ggcaccccgc aattcccgat gtgcgggttc tccagccgtt cgctgcaggc
    19921 gctgatggcc gccggcgcgc agctgcacac cgtcaacgtg ctggaggagc cggagatccg
    19981 cgccaacctg ccgcgctact cggactggcc gaccttcccg cagctgttca tcaacggcga
    20041 gctgatcggt ggctgcgaca tcaccgtcga gctctccgaa tccggcgagc tggcgcggat
    20101 cgtcgcggac gcgcagaagg cgtgagcggc gcttcgtcgc ttgagggccg cgtcatcctg
    20161 gtcgccggcg ccgccggtgg cctgggctcg gccgcgtccg tggcctgcgc ccaggccggc
    20221 gcgacggtgg tgctgctcgg gcgcaaggtc gccccgctga accgggtgta cgacgcggcc
    20281 agggccgtcg gcccggaacc ggtcctgtac ccgctggacc tcgaaggcgc ctccccggac
    20341 gatttcgacg agctcggcca gcgcatcgaa gccgacctcg gccgcctcga tggactgctg
    20401 cactgcgccg ccgagttcaa gggcctgacc ccgttgcagc acaccgaccc ggccgatttc
    20461 gcccgcgccc tccacgtcga cctgaccgca cgctggtggc tcacccaggc ctgcctgcca
    20521 ctgcttgcga agtccgacgc cggcgcggtg gtgttcgcca tcgacgaccc agcccgcaac
    20581 ggcagccctg ccttctgggg cggctacggc ctggcccagt cggcgcaggt caccctggtg
    20641 ccgatgctgc aggcggaact cggcgagcac ggtccacgcg tcagtggcct gcgcccggga
    20701 ccgatgcgca ccggcctgcg cggcaaggcg ttccagccga ataccgacct caaggcgaag
    20761 cccgccagcg cctatgccgc cgcctgcgtc gaactgctgt ccgcggccgg cgccgcgcag
    20821 cgcgggcgga tcgtggacgt gctggcgtga ccatcgccgc cgccgccctg ctgctgttcc
    20881 tgatcctgga tccgctgggc aacatcccgg tgttcctgag cctgctcaag gggttgcctc
    20941 cagcacgcca gcgggtggtg atcgtccgcg agctgctgat cgcgctcggc gtgctgatgc
    21001 tgttcctgtg gaccggccgc ttcctgctgg aactgatgca cctgcgccag gaatcggtgt
    21061 cgatcgccgg cggcatcatc ctgttcctga tcggcctgcg gatgatcttc ccgtcggccg
    21121 acgggatcat gggcgaacag ccggaagggg agcccttcat cgtaccgatg gcgatcccga
    21181 tgatcgccgg cccctccggg atggcggcgg tgatgctgct gggcagccag gaaccgggcc
    21241 ggatgggtgc ctggagcctg gccctggcga tcgcctgggc cgccaccgcg atcatcctgt
    21301 tttccgcgac ctacctgcag aaatggctgg gaacgcgcgt gctgaccgcg atcgagcggc
    21361 tgatgggcat ggtcatcgtg gcgatctcgg tgcagatgct gctggacggc atcgccagct
    21421 acctccgcgc aggaagctga acaggggcaa ggacttacgc tgggtgaccg gcgtcgccgc
    21481 actgtcacga agccgtcatc cggtgtcgct accgtgttaa actttagtga acccatgcgg
    21541 cgtcgtatgg gcaggggttc cgaattcgcc gacaaccgtc gcccaggcgg acgcgtcggc
    21601 atgtgccgtc cttcgtccgt cgttcttttc tttatttgct cacttgaggt taccgatatg
    21661 actcacccga accgcgtccg gctttcgaag ctgtcgctgg gcctgatggt cgcgcttgcc
    21721 gcggctcctg ttttcgcgca gagcacctcc gccggcgttg gcggccaggt tgtcggtgcc
    21781 gacggccagc cggtcgccgg cgccgaagtc accatcaccc acatcgactc gggcacggtc
    21841 agccgcgcca ccaccgatgc cgccggtcgc tacaacgcac gcggcctgcg cgtgggcggt
    21901 ccgtacaccg tcaccatcaa caaggcgggc gcgggcagcg ataccgaaga gaacgtgtac
    21961 ctgaccctgg gtaccgcgaa cacgatcgac gcgcagctga acaacgacgt cgcgaccctg
    22021 ggcaccgtgc aggccgtggc caccgccggg acctcagtgt tctcggccac caagatgggt
    22081 gctggcaccg ccgtggacca gaacaccatc aaggcgctgc cgtcgatcaa cggcaacatc
    22141 caggacttca tgcgcctcga tccgcgcgtc gcctttaccg atcgcgcgtc gggcagcatg
    22201 agtgccggtg gcctgaaccc gcgcttcaac aagatcacca ttgacggcgt ctccgccagc
    22261 gatacgtttg gcctggaagg caacaacatg ccgacccagc gccagccggt cgccctggag
    22321 gccatcgagg cactcgacat caacctgtcg aactacgatg tgaccatgtc aggcgctgcg
    22381 ggcgcgaacg tcaacgcggt caccaagtcg ggcaccaacc agttccacgg cgcgctgtac
    22441 gggaattacc gggacggcga ctggtttggc gactatccgg cgcgcgtggc gggtagcccg
    22501 ctcgacgtga ccggcgtgcc gttcgacgag ttcgacgaag agaaaacctg gggcttcacc
    22561 ctgggcggtc ccatcatcaa ggacaagctg ttcttcttcg ccaactacga gaagttgacc
    22621 cagaccaaca tcacctccgc cggtggcaag agcgtggcca gcaacccgct gatcgtcacc
    22681 ggcgccgatt tcggtgccgc cgaagtagct gaagtccagc gcatcgcgga agaggtctgg
    22741 ggcttcgacg ccggcggcct gatcggcggc aacaccgaac tggaagaata cgcagtcaag
    22801 ttcgactgga acatcagcga gaaccaccgc gccaacctgc gttacagcaa gctggagcag
    22861 gtgcgcgtgc ggcctgaagc ctccaccgcc ggctcgctgt cactgagttc caactggttc
    22921 aaccacgaga agaccgtgga
//
LOCUS       DAORUK010000017        22768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_1852_length_22768_cov_18.298419, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000017 DAORUK010000000
VERSION     DAORUK010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 22768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22768
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1852_length_22768_cov_18.298419"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /gene="rimO"
                     /locus_tag="PK818_02190"
     CDS             <1..1305
                     /gene="rimO"
                     /locus_tag="PK818_02190"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HQY81577.1"
                     /translation="FVSLGCPKALVDSERILTQLRVEGYDLVQRYDDADVVVVNTCGF
                     IDSAVAESLDAIGEAMAENGKVIVTGCLGKRSEVIREAYPDVLSISGPQDYASVMDAV
                     HLALPPKHDPFIDLLPDYGLKLTPKHYAYLKISEGCNHRCSFCIIPSMRGDLVSRPVD
                     EVLREAEKLVRGGVKELLVVSQDTSAYGVDLKYATRLWRGNAYQTRMKALCEGLSGLG
                     AWTRLHYVYPYPHVDEIIPLMAEGKLLPYLDIPFQHASPRILKLMKRPGAVDKTLERI
                     QRWRAACPELTIRSTFIVGFPGETDAEFEELLEFLDEAQLDRVGAFAYSPVAGAQANA
                     LPDPVDEGVKQERLARFMERQAAISEARLAAKVGSVQQVIVDAIDGDLAIARSMADAP
                     EIDGLVQVQDGREAGLQPGEFAMVRIMGSDEHDLYGEVEYEG"
     gene            1370..2128
                     /locus_tag="PK818_02195"
     CDS             1370..2128
                     /locus_tag="PK818_02195"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HQY81578.1"
                     /translation="MGTDSKNTTRQLRRPGWARLLRRGNDHLMFDLFGGPRPWKLSWL
                     INLQKAGTFPFLGLLMWAYADRTPAATAPAAWIYLAMHGSYGLVWLLKDLAFPDPGWQ
                     RRSTIAGGLATFAGLLAYWSFGWLLVSGTATPRYPLPEPAWFALCVSACILGCALMIA
                     ADAQKYFTLRLQPGLITDGVHRYIRHPNYLGEMLVYGSFALMVWHWWPFAVLALIWGG
                     LFAPNMAMKEASLSRHPGWHAYRQRSWWLLPFLL"
     gene            complement(2165..2671)
                     /gene="greB"
                     /locus_tag="PK818_02200"
     CDS             complement(2165..2671)
                     /gene="greB"
                     /locus_tag="PK818_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007963950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HQY81579.1"
                     /translation="MGRWRPPAEHGTALITREGHDRLKAELDDLWRVQRPEVVRALAA
                     AAAEGDRSENAEYTYRKKQLGGIDRRVRYLSRRLQALRVVDAAPSDRDAVFFGAEVTL
                     EDIGSGELALYRIVGPDETDAQRGWISIDAPLARAMLKKRVDDEFDALLPTGIAHFAI
                     VGVAYPNG"
     gene            complement(2690..3193)
                     /locus_tag="PK818_02205"
     CDS             complement(2690..3193)
                     /locus_tag="PK818_02205"
                     /inference="COORDINATES: protein motif:HMM:NF016087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE"
                     /protein_id="HQY81580.1"
                     /translation="MNRHPLLIALAIAAAVSAIAASGCKRAPEAPAQVAAAPAEAIDT
                     AVVAEANHATPDSAAPDFDAKAFAGLYAATLPCADCPGIDTSISFAAEGTYSMSETYQ
                     DSADSSFVTKGTWSVREDGKTVVLDPEDKQERDRWFQVVSATELRALDLEGKPIESTL
                     DYSLRRK"
     gene            complement(3243..4769)
                     /locus_tag="PK818_02210"
     CDS             complement(3243..4769)
                     /locus_tag="PK818_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HQY81581.1"
                     /translation="MQQAPVPSILVGKAITTDSSGQVHLLPKYGNRHGLVAGATGTGK
                     TVTLMTLAEGFSRIGVPVFLADVKGDVAGLAVAGTTSEKLQARLAQIGISDYRNEPAP
                     VVFWDMWGKSGHPVRTTVSEMGPTLLGRILELNDTQGGVLDIVFKLADDRGLLLLDLE
                     DLRALLGLIADERKDISTTYGLVSTQSIGAIQRALLRLEAEGAELFFGEPALELADLM
                     RTTTDGRGVVNILAADQLILKPKLYSSFLLWLLSELFETLPEVGDMDKPKLVFVFDEA
                     HLLFDDAPPALRQRIEQVVRIIRSKGVGVYFCSQFPDDVPDEILGQLGNRFQHALRAY
                     TPRDQKAVRTAAETFVANPALDVAQAIGKLGTGEALVSTLQEKGVPMAVEHTLVAPPR
                     CRMGAITDAERMQVRAGSPVGIKYDTRIDRDSAAEMLAKKAGQKAEQAQAPQARTRED
                     DEGEQGGFGQAVKDAVFGTRRRQGMIETAAKQTARTVGSQVGRQILRGLLGGIFGGKR
                     "
     gene            4908..6434
                     /locus_tag="PK818_02215"
     CDS             4908..6434
                     /locus_tag="PK818_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005914309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQY81582.1"
                     /translation="MPPRLLPLAAALLLSFLAVLSSLPAQAQSKRDTAASAALQQRMD
                     AAEKHYRDAMLAEDEEAAARASSAALSEMKAVIEACSKQRGCSVPTMLATYERLLKAT
                     PQLGEEVFDDEGEDPIDEGDFASADVPESANAASLLSEDGQRFVRMVQFNPAVQAGIR
                     RWLTDMRPSLMDSYENYRYMQHLMSPSFKRSGLPEALLFGIMAKESNGKVHVGSRAGA
                     VGPLQFMPATGSRFGLGNDGTGFDTRYDPRASADAAAAFLNERMAGLGNSIELSLAGY
                     NGGEGRAARVYRESGGRSFWDADVYNQFPAETKDYVPMVIAAAWLFLHPKEYGIRWPR
                     VSTRQATLQLAQPASIYELTICLGNYGSRDGYMRALRNLNPRWEADTTLPAGTSLNAT
                     ARIAWLYRFNCRGGKRAELAKQLVASNVQSALVRVGPMTNPGDATASAPTQDAAPPPR
                     PVKPAKPKTHKVQRGETLSSIARKFDCDLAGIAKANKLKAPKYAIRPGQRLTLAGCGG
                     "
     gene            6520..6981
                     /locus_tag="PK818_02220"
     CDS             6520..6981
                     /locus_tag="PK818_02220"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HQY81583.1"
                     /translation="MSALDHRIPPPLVGLACGLLAWLLARASPGLGVDWLPRVPVAAA
                     LVALGLSLDVWGLVAFRRARTTLNPLSPERTSAVVQGGPYRFTRNPMYLGMACVLLGA
                     CAWLANPLALLAVFVFVGYITRFQIMPEERALLGKFGGAYADYLRRVRRWL"
     gene            complement(7022..7864)
                     /gene="asd"
                     /locus_tag="PK818_02225"
     CDS             complement(7022..7864)
                     /gene="asd"
                     /locus_tag="PK818_02225"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347956.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HQY81584.1"
                     /translation="MSPLTALTYVLPHRALSSLARALAYSTNPSLKQWLIDTVTRKFG
                     VDLSEAAEPDPRAYPTFNAFFTRALKPGARVADPDPRALLMPADGHVSQCGDIVDGRI
                     FQAKGQAYTAAELLGSEADAAPFADGVFATVYLSPRDYHRVHMPWAGTLRETVHVPGR
                     LFSVGTDAVASVPRVFARNERLVCHFDTEFGPMVQVMVGALLVSGVETVWSGVEIPAY
                     GDAITRKDYRGKGIALERFAEMARFNYGSTVIVLLPRGAAMLAAGLAAETPVRLGQRL
                     ATRA"
     gene            complement(7861..8499)
                     /locus_tag="PK818_02230"
     CDS             complement(7861..8499)
                     /locus_tag="PK818_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HQY81585.1"
                     /translation="MSKRALVLLVIATLAAGLGLWASQRHFAGRGSSVPAVRSMTLLP
                     EPRSLPAFALQQSDGTPLTADELKGHWTLVFLGFTHCPDVCPTTLAQLAGAQKQWASL
                     PAATRPRVLFVSADPERDSAELTGTYAHGFHPDTLAATAPVPQLEAFARSLSLVFMKA
                     PGPSGDPGDYSIDHSAGLVVLDPQARMAGVAQQPLDVTSIAADMASLTKAVR"
     gene            8596..9543
                     /gene="prmB"
                     /locus_tag="PK818_02235"
     CDS             8596..9543
                     /gene="prmB"
                     /locus_tag="PK818_02235"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQY81586.1"
                     /translation="MSEELQTIIDLIRYGASRFNAAGLTFGHSYDNALDEATQLTLHA
                     LHLPHDLSPVYGNARVTEAEKEDVLGLFLRRIEERIPAAYLTGEAWFAGLSFKSDPRA
                     LVPRSPIAEMIEAGFQPWLGDREVRRALDLCTGSGCIAIAMAHYNPEWHVDGVDISED
                     ALALSGENERRLQVENVRFLKSDLFSGLTGEHYDLIVTNPPYVTNDETDALPREYSHE
                     PELGLRAGDDGLDLALRIMRDAPLHLDRDGLLVCEVGEAERALVKLLPELPLAWVEFK
                     VGQMGIFVAECADLLAHHAHIARLADAREARRQPPGDLF"
     gene            9572..10675
                     /gene="aroC"
                     /locus_tag="PK818_02240"
     CDS             9572..10675
                     /gene="aroC"
                     /locus_tag="PK818_02240"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HQY81587.1"
                     /translation="MNSFGHLLRVTTFGESHGPAIGCVIDGCPPGIAIAPEDFVQDLA
                     RRATGTSRHTSQRHEADAVEILSGVYEGLTTGTPIALLVRNTDARSKDYSRIGEQFRP
                     GHADYTYWHKYGIRDPRGGGRSSARETTMRVAAAVIAKKWLADRYGVRVQGWLSQIGD
                     IVPAGFDAGVIETNPFFWPHAPQVGELESYMDALRKSGDSVGARVTVVADGVPPGWGE
                     PIYGKLDGELAAAMMSINAVKGVEIGAGFASVSQKGSEHRDEATRDGFASNHAGGILG
                     GISSGQQVLVSVAFKPTSSLRLPGRGMDVHGEETEIVTTGRHDPCVGIRATPICEAML
                     ALVLMDQALRHRAQCGDVGAVSPRIPAGMDPKR"
     gene            10839..11855
                     /locus_tag="PK818_02245"
     CDS             10839..11855
                     /locus_tag="PK818_02245"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HQY81588.1"
                     /translation="MSKQFKVAVVGATGAVGEVMLSILAERDFPASEVVALASERSAG
                     SRVAFGNGEIVVQDLATFDPAGIDIALFSAGGSTSREYAPKFAAAGAVVIDNSSAFRY
                     DDDVPLVVSEVNPGQVANRPRGIIANPNCSTMQMLVALKPLHDAAGIQRINVATYQSV
                     SGGGRSALEELGRQTGALLNFQDPDPQRFPVQIAFNLIPHIDDFLDNGYTKEEMKLVW
                     ETRKILGDESIQVNPTAVRVPVFFGHSEAVNIETRDKLTAAQARELLERAPGVEVVDE
                     RGPGGYPTPVTHASGTDPVYVGRIREDLSHPRGLNLWIVSDNIRKGAALNAVQIAELV
                     ASEG"
     gene            11916..13967
                     /locus_tag="PK818_02250"
     CDS             11916..13967
                     /locus_tag="PK818_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015463396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HQY81589.1"
                     /translation="MAVLTGLLLAAGNAAALGLGQIEVKSRLNQPLLAEIPIISTTPG
                     ELQALQARLASPETFRRIGLAAPTGAAANLQFSLGSDARGRPVIRVTTVAPVDQAVLN
                     FLIEVDWGQGRLVREYSALVDAPRTASAPVQPAISAPVVAAPNVVQRPDTPPVAALPV
                     PVPPPAPPPPPPPPPAPPAPPAQPAVEPEPVAVAVEPLPPPVAVVPAPPPARVLPPAP
                     ARSVAVAEPPPAPAPAAANGRYGPVKQGETLSKIAGALDVAAGYSLDQTMLALLRANP
                     DAFLGDDINRLKRGAVLRVPEPGELGSISPDEAALVVREQMQQWRQARRPVAQPDTAV
                     AVAEAPSRTAAAAAKPAEAKSTAAKPLAAKPAEARPAVAKPAAPAAQRRQQARLEIVP
                     PSAPGKATGTRSGTNAGGEGSMLQQQLRQKDEDIAAKAAEIGDLKEQVAELEKIRQQQ
                     EQLLSLKDSELAAAQQRLAAARAAAAATPAMPTAAATQATQASQSTPAAEPPPANSMP
                     WLWGGLALVGMALLAWLFVRRQPKRQPKAGARRGFDSEALAASMVVPTVEDVPRDTGI
                     DDAEDAATSVTTEVDLDQVPAAAGTRVETPNWHSGWVKADPPAPAPAPEAEPPTPRFV
                     PQESAPPPPQASPEQRMKLARAFLDIGDDHSARELLRELLDGPDPAARSEAARLLREL
                     G"
     gene            13987..14772
                     /gene="truA"
                     /locus_tag="PK818_02255"
     CDS             13987..14772
                     /gene="truA"
                     /locus_tag="PK818_02255"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HQY81590.1"
                     /translation="MRLALGVEYDGSGFSGWQRLSAAGSREPDGSLQTALEAALSRVA
                     DARIDTICAGRTDAGVHGQCQVVHFDSDVVRDARGWVLGTTANLPPTMAVRWCVPVAG
                     DFSARFSARARRYRYRILNRSTRPGLQRQYLGWERMPLDAQAMHAAAQALVGERDFSA
                     FRSVQCQATHARRDLQEISVHRRGAEVVVEVQANAFLHHMVRNIVGSLLVVGRGERPP
                     AWIAELLERRDRTVAGPTAPAAGLLFLGPRYPRGCGLPDEVSL"
     gene            14805..15407
                     /locus_tag="PK818_02260"
     CDS             14805..15407
                     /locus_tag="PK818_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HQY81591.1"
                     /translation="MTRAEDVELAVALGVDYIGLVFAGGSPRHLALAQAQPLRALVPA
                     SVGVVALVMDAPRAEVEAIASTLRPDCLQFHGSEEDRFCASFGMPYWKAIAMGDGADP
                     ALAMARFPSARGFVLDGHGAGEAGGSGRSFDWTRWPRDQHRPVLLAGGLSAQNVALAL
                     RTARPWGVDVSSGIEQAPGAKDAAHMRAFVAAVRAHDAAS"
     gene            15633..16871
                     /gene="trpB"
                     /locus_tag="PK818_02265"
     CDS             15633..16871
                     /gene="trpB"
                     /locus_tag="PK818_02265"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HQY81592.1"
                     /translation="MNTPAPSTAPAVAIDFNQYPDASGHFGRYGGRFVAETLMGPLDE
                     LAAAYDAAKVDPAFIAEFEADLAHYVGRPSPIYHAERLSREAGGARILLKREDLNHTG
                     AHKINNTIGQALLASRMGKTRIIAETGAGQHGVASATVAARLGLECVVYMGATDIQRQ
                     KINVYRMQLLGATVVPVTSGSATLKDALNEAMRDWVSNVRDTFYIIGTVAGPDPYPRM
                     VRDFNAVVGREARAQMLAEYGRLPDVVTACVGGGSNAIGIFHAFLNDAGVRIVGAEAA
                     GEGIASGHHAASLAAGRPGVLHGNRTYVICDDDGQIVETHSVSAGLDYPGVGPEHAFL
                     KDSRRADYVGVTDDEAMAAFHLLARTEGILPALESSHAIAQAIKLARELPKDGIVLCN
                     LSGRGDKDVHTIAGREGLAL"
     gene            16942..17388
                     /locus_tag="PK818_02270"
     CDS             16942..17388
                     /locus_tag="PK818_02270"
                     /inference="COORDINATES: protein motif:HMM:NF022786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HQY81593.1"
                     /translation="MADEQAQQEALAQPAAADNQLDPAFFTAVNEYLELTNKQSRQHG
                     LKRISLASLYAAARFNSHVFLSNVAPGQAAGDRAHFLDYMATMYRRMLNEHLDGLGHE
                     RGIDVGESELAAEYAAAGVQVGRMGQTPAGDYVAAAGATPPAASDE"
     gene            17385..18215
                     /gene="trpA"
                     /locus_tag="PK818_02275"
     CDS             17385..18215
                     /gene="trpA"
                     /locus_tag="PK818_02275"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HQY81594.1"
                     /translation="MSLPRHARLQARLDALESAGRKALVPFVTAGDPSLAATVPVMHA
                     LVEAGADVLELGVPFSDPMADGPVIQRSSERALARGAGIRYVLECVRGFRERDQATPV
                     VLMGYLNPVEIRGPASFAAAAADAGVDGVLLVDLPPEEADEYRAAFALNGLALISLAS
                     PTTPEARLQRLVTQADGYLYYVSFAGVTGADRLDVGDAGQRLQGLRTMAAIPVYAGFG
                     IRDAHSAAAMAAHADGVVVGSALVSALDGSLDEAEAAQRAVAFLRPLRQALDGLAVAA
                     "
     gene            18352..19239
                     /gene="accD"
                     /locus_tag="PK818_02280"
     CDS             18352..19239
                     /gene="accD"
                     /locus_tag="PK818_02280"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HQY81595.1"
                     /translation="MSWLKKLMPARIRTDTGVDRKRSVPEGLWEKCERCGAVLYRPEL
                     EEHLEVCPKCSFHMPIRARARLAALLDDGMGHELGAALGPTDVLKFRDQKKYADRIKA
                     AQKSTGERDALVAMEGRLKGRELVAAAFDFAYMGGSMGSVVGERFTLAAERALELRCP
                     FVCFSASGGARMQESLFSLMQMAKTSAALGRLRAAGLPYISVLTHPTTGGVSASFAML
                     GDLNIAEPEALIGFAGQRVIEQTVREKLPEGFQRSEFLLAHGTVDQIVDRREMRERLA
                     HVLAILMKQPAPAEDAEAA"
     gene            19236..20600
                     /gene="glmM"
                     /locus_tag="PK818_02285"
     CDS             19236..20600
                     /gene="glmM"
                     /locus_tag="PK818_02285"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQY81596.1"
                     /translation="MSARRYFGTDGIRGRVGDGPISADFVLRLGNAYGHALTAAAAGG
                     DRIWRKPMVVIGKDTRISNYMFEAALEAGLVAAGVDVQLMGPMPTPAVAHLTHSMRAD
                     GGIVISASHNPHHDNGIKFFSAQGEKLDDATELAIEAALDAPFRTVPSGQLGKAVRTR
                     DTIGRYVEACKNSVPRGFNLGGMRIVLDCANGATYQLGPLVLRELGARVDAMGVEPNG
                     VNINDGVGSTHPEGLAARVRETGADIGIAFDGDGDRVLFVDAAGNVRDGDDLLYVLAC
                     DWQESGRLRGPVVGTLMTNYGFEQALAARGIGFVRAKVGDRHVHQQLLANDAVLGGEA
                     SGHLLCLDRASTGDGIVSALQVLEVLRRRGVGLAEALQGLRKVPQKTINVRCEGGRKP
                     ADADSVKAALAAAQGSIEGRGRAFLRASGTEPVVRVTVEADDAALVDATLAVLADAVR
                     AAAA"
     gene            20681..21634
                     /locus_tag="PK818_02290"
     CDS             20681..21634
                     /locus_tag="PK818_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HQY81597.1"
                     /translation="MSPRIPTLDIRRFTDPASPQDRQAFIDELGAAYREWGFAGIRNH
                     GIPQARIDEAYDTFRRFFALPEATKRQYHVAGGGGARGYTPFGVETAKGAQYSDLKEF
                     WHIGREIPRDSKYANDMPANVWPQDMPEFQSVGYGLYQSLDALGSQVLRALALHIGLP
                     EDYFADKTDSGNSILRPIHYPPITVDDIPNVRAGAHEDINLVTLLVGASAEGLEVLSR
                     KGEWVPFTADADTIVVNIGDMLQRLTNHVYPSTTHRVVNPPGEKARQPRYSTPFFLHP
                     NPDFLIDVLPSCVSADNPSRYPQPITAQGYLEERLREIKLK"
     gene            21661..22413
                     /gene="tpiA"
                     /locus_tag="PK818_02295"
     CDS             21661..22413
                     /gene="tpiA"
                     /locus_tag="PK818_02295"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HQY81598.1"
                     /translation="MRRRIVIGNWKLHGDRAFAHRLVDAIAAVPAAAGVERVILPPLP
                     YLGELVAHCAGTDLRFGAQDVSANVQGAYTGEVSASMLVDIGAVYGLVGHSERRQHHR
                     EISELVARKFVAAKDAGLTPILCVGESLHEREAGQTEWRIEEQLQAVFDLAGPQAFAG
                     AIIAYEPVWAIGTGRTASPAQAQAVHAFIRGEVAARDATIAGSLPILYGGSVKPDNAA
                     SLFAQADVDGGLIGGASLVAADFLAIASAAAA"
     gene            22437..>22768
                     /gene="secG"
                     /locus_tag="PK818_02300"
     CDS             22437..>22768
                     /gene="secG"
                     /locus_tag="PK818_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HQY81599.1"
                     /translation="MLLQLINVLYVLVAIAMIGLILMQRGAGAQAGSGFGGGASATVF
                     GSRGASNFLTKSTKWLAIVFFTISMGMAVYASKSARQGGAEPAAADLGVMGQAAPAAP
                     ARQVPSAQV"
BASE COUNT         3404 a         7940 c         8099 g         3325 t
ORIGIN      
        1 ttcgtcagcc tcggctgccc caaggcgctg gtcgattccg agcgcatcct cacccagttg
       61 cgggtggagg gctacgacct ggtccagcgc tacgacgatg ccgacgtggt ggtggtcaac
      121 acctgcggct tcatcgacag cgcggtggcc gaatcgctgg acgcaatcgg cgaggcgatg
      181 gcggagaacg gcaaggtgat cgtcaccggt tgcctgggca agcgcagcga ggtgatccgc
      241 gaggcctacc cggacgtgct gtcgatcagt ggcccgcagg actacgcaag cgtgatggat
      301 gcggtgcacc tggcgctgcc gccgaagcac gatccgttca tcgacctgct gccggactac
      361 ggcctgaagc tgaccccgaa gcactacgcc tacctcaaga tttccgaggg ctgcaaccac
      421 cgctgcagct tctgcatcat cccgtcgatg cgcggcgacc tggtctcgcg cccggtggac
      481 gaggtgctgc gcgaggccga gaagctggtg cgcggcggcg tcaaggaact gctggtggtc
      541 tcgcaggaca ccagcgcgta tggcgtggac ctcaagtacg cgacccgcct gtggcgcggc
      601 aacgcctacc agacccggat gaaggcgctg tgcgagggcc tgtccgggct cggcgcgtgg
      661 acgcggctgc attacgtgta tccgtatccg cacgtggacg agatcatccc gctgatggcg
      721 gagggcaagc tgctgccgta cctggacatc ccgttccagc atgccagccc gcgcatcctc
      781 aagctgatga agcggcccgg cgcggtggac aagaccctgg aacggatcca gcgctggcgg
      841 gccgcatgcc cggagctgac catccgcagc actttcatcg tcggcttccc cggcgagacc
      901 gacgccgagt tcgaggaact gctggagttc ctcgacgaag cccagctcga ccgcgtcggc
      961 gcgttcgcct attcgccggt ggccggcgcg caggcgaacg cgctgccgga cccggtggac
     1021 gaaggcgtga agcaggaacg cctggcccgg ttcatggaac gccaggccgc gatcagcgaa
     1081 gcccgcctcg ccgcgaaggt ggggtcggtg cagcaggtga tcgtggatgc catcgacggc
     1141 gaccttgcga tcgcgcggtc gatggcggat gcgccggaga tcgacggact ggtgcaggtg
     1201 caggacggcc gcgaggccgg gctgcaaccc ggcgagttcg caatggtccg gatcatgggc
     1261 agcgacgaac acgacctcta cggcgaagtc gaatacgaag gctgagggtt cctgcgggac
     1321 gcccggcgag tcgcctgcag gtcgcgggca cgaacgagga gatggcggga tgggcaccga
     1381 ctcgaagaac acgacccgcc agctgcgccg acccggctgg gcgcggttgc tcaggcgtgg
     1441 caacgaccac ctgatgttcg acctgttcgg cggcccgcgc ccgtggaagt tgagttggct
     1501 gatcaacctg cagaaggcgg gcacgttccc gttccttggc ctgttgatgt gggcatacgc
     1561 cgaccgcacg ccggccgcga ccgcgccggc ggcctggatc tacctggcga tgcatggctc
     1621 gtacggactg gtctggctgc tcaaggacct cgccttcccc gaccccggct ggcagcgccg
     1681 cagcacgatc gctggcggcc tggcgacgtt cgccggcctg ctcgcctatt ggtcgttcgg
     1741 ctggctgctg gtttcgggta ccgccacgcc gcgctatccg ttgccggagc ccgcctggtt
     1801 cgcgttgtgc gtgtcggcct gcatcctcgg ttgcgcgctg atgatcgccg ccgacgcgca
     1861 gaagtacttc acgctgcggc tgcagccggg gctgatcacg gatggcgtgc atcgctacat
     1921 ccgccatccc aattacctgg gcgagatgct ggtgtacggg tccttcgccc tgatggtctg
     1981 gcactggtgg ccgttcgccg tgctcgcgct gatctggggc gggctgttcg cgcccaacat
     2041 ggcgatgaag gaagccagcc tgtcgcgcca tccgggttgg cacgcgtatc gccaacgcag
     2101 ctggtggctg ctgcccttcc tgctctgaac gcggcgcagg gatgcgggcc ggacccgcat
     2161 cgcctcagcc gttcggatac gccacgccga cgatcgcgaa atgggcgatg ccggtgggca
     2221 gcagcgcatc gaattcgtcg tcgacgcgct tcttcagcat cgcccgcgcc agcggcgcgt
     2281 cgatgctgat ccagccgcgc tgcgcatcgg tttcatcggg gccgacgatg cggtaaagcg
     2341 ccagttcgcc gctgccgatg tcctccagcg tcacttcggc gccgaagaac accgcgtcgc
     2401 ggtcggatgg cgcggcatcg accacccgca gcgcctgcag ccgcctggag agatagcgca
     2461 cgcggcggtc gatcccgccc agctgcttct tgcggtaggt gtattcggcg ttctcggagc
     2521 ggtcgccttc ggccgccgcc gccgccagcg cacgcaccac ctccggccgc tgcacccgcc
     2581 acaggtcgtc cagctccgcc ttgagccggt catggccttc gcgggtgatc agggcggtgc
     2641 cgtgctcggc gggcgggcgc cagcgaccca tggctgcggc gccgcggcct tacttgcggc
     2701 gcaggctgta gtcgagcgtg ctctcgatcg gcttgccctc caggtccagc gcgcgcagct
     2761 cggttgccga caccacctgg aaccagcggt cgcgctcttg cttgtcctcg gggtcgagca
     2821 ccacggtctt gccgtcctcg cgcacgctcc aggtgccctt ggtcacgaag ctgctgtcgg
     2881 cgctgtcctg gtaggtctcg ctcatcgaat aggtgccttc ggcggcgaag gaaatgctgg
     2941 tgtcgatgcc ggggcagtcc gcgcagggca gggtggccgc gtacaggccg gcgaaggcct
     3001 tggcgtcgaa gtccggcgcg gcgctgtccg gcgtggcgtg gttggcttcc gcgaccaccg
     3061 cggtgtcgat tgcttcggcg ggcgcggccg cgacctgcgc aggcgcttcc ggtgcgcgct
     3121 tgcagccgct tgcggcgatg gcggaaacgg cggcggcgat ggccagggcg atcagcaggg
     3181 ggtgtcggtt catggggtgg ctcgttggtg ggttcgtggg gtgtttcggg aacgaggggc
     3241 ggctagcgct tgccgccgaa aatgccgccc agcaggccac gcaggatctg ccggcccacc
     3301 tggctgccga cggtgcgcgc ggtctgcttg gcggcggtct cgatcatgcc ctggcggcgc
     3361 ctggtgccga acaccgcgtc cttcaccgcc tggccgaagc cgccttgctc gccctcgtcg
     3421 tcttcgcggg tcctggcttg cggtgcctgc gcctgttcgg ctttctgccc ggccttcttc
     3481 gccagcattt cggcggcgga atcgcggtcg atacgggtgt cgtacttgat gccgaccggg
     3541 ctgccggcac gtacctgcat gcgttcggca tcggtgatcg cacccatccg gcagcgcggc
     3601 ggggcgacca gggtgtgctc gaccgccatc ggcacgcctt tttcctgcag ggtggacacc
     3661 agcgcctcgc cggtacccag cttgccgatc gcctgcgcca cgtccagcgc cgggttggcg
     3721 acgaaggtct cggcggcggt gcgcaccgcc ttctggtcac gcggcgtgta ggcgcgcagc
     3781 gcgtgctgga agcggttgcc gagctggccc aggatctcgt ccgggacgtc gtccgggaac
     3841 tgcgagcaga agtacacgcc cacgcccttc gagcggatga tccgcaccac ctgttcgatc
     3901 cgctggcgca gcgccggcgg cgcgtcgtcg aacagcaggt gcgcctcgtc gaacacgaac
     3961 accagcttcg gcttgtccat gtcgcccact tccggcagcg tctcgaacag ctccgacagc
     4021 agccacagca ggaaactgga atacagcttc ggcttcagga tcagctggtc ggcggcgagg
     4081 atgttgacca cgccgcggcc gtcggtggtg gtgcgcatca ggtcggccag ttccagcgcc
     4141 ggttcgccga agaacagctc cgcgccctcg gcttccagcc gcagcagcgc gcgctggatc
     4201 gcgccgatcg actgcgtgct gaccagcccg tacgtggtgc tgatgtcctt gcgttcgtcc
     4261 gcgatcaggc ccagcagcgc gcgcaggtcc tccaggtcca gcaacagcag gccacggtcg
     4321 tcggccagct tgaacacgat gtccagcacc ccgccctggg tatcgttcag ttccaggatg
     4381 cggccgagca gggtcgggcc catttcgctg accgtggtgc gcaccgggtg cccggacttg
     4441 ccccacatgt cccagaacac caccggggca ggctcgttgc ggtaatcgct gatgccgatc
     4501 tgggccagcc gcgcctgcag cttctcgctg gtggtgccgg ccaccgccag cccggcgacg
     4561 tcgcccttga cgtcggccag gaacaccggc accccgatcc ggctgaagcc ttcggccagg
     4621 gtcatcaggg tcaccgtctt gccggtgccg gtggcgcccg ccaccagccc gtggcggttg
     4681 ccgtacttcg gcaacaggtg gacctggcca ctgctgtcgg tggtgatggc cttgccgacc
     4741 aggatcgacg gaacgggcgc ctgctgcatg ctgttgcctc ggctgcacat gaatgcgtgg
     4801 agcgccgatt ctatgcgatg cgcgcgggcc tgccgtgacg tgcggcggtt gccccgccgc
     4861 aggcgtggcg gctaccctgc ggcatcccct tccttgcccc tcgcctgatg cccccacgcc
     4921 tgttgccgct cgccgccgcg ctgttgctgt cgttccttgc agtcctgtcc agcctgcccg
     4981 cgcaggccca gtccaagcgc gacacggccg cgtcggccgc gctccagcaa cgcatggacg
     5041 ccgccgagaa gcactatcgc gatgccatgc tggccgaaga cgaggaagcc gccgcccgcg
     5101 ccagcagcgc ggcgttgtcc gagatgaagg cggtgatcga agcctgctcg aaacagcgcg
     5161 gttgttcggt gccgaccatg ctggccacct acgagcgcct gctgaaggcg accccgcaac
     5221 tgggcgagga agtgttcgac gacgagggcg aggatccgat cgacgaaggc gatttcgcct
     5281 cggccgacgt cccggagtcc gcgaacgccg cctcgctgct gagcgaggat ggccagcgct
     5341 tcgtgcgcat ggtccagttc aacccggcgg tgcaggccgg catccgccgc tggctgaccg
     5401 acatgcggcc gtcgctgatg gacagctacg agaactaccg ctacatgcag cacctgatgt
     5461 cgccgtcgtt caagcgcagc gggctgccgg aggcgctgct gttcgggatc atggccaagg
     5521 aatccaatgg caaggtgcac gtgggctcgc gtgccggcgc ggtcgggccg ctgcagttca
     5581 tgcccgccac cggcagccgc ttcgggctgg gcaacgacgg caccggcttc gacacccgct
     5641 acgacccgcg cgcctccgcc gacgccgcgg cggccttcct caacgagcgc atggcgggcc
     5701 tcggcaacag catcgagctg tcgctggccg gctacaacgg tggcgagggg cgggcggcgc
     5761 gcgtctaccg cgagagcggc gggcgcagct tctgggatgc ggacgtctac aaccagttcc
     5821 cggcggaaac caaggactac gtgccgatgg tgatcgccgc cgcgtggctg ttcctgcacc
     5881 cgaaggaata cggcatccgc tggccgcggg tcagcacccg ccaggccacc ctgcagctgg
     5941 cgcagccggc ctcgatctac gaactgacca tttgcctggg caactacggt tcgcgcgacg
     6001 gctacatgcg cgcgctgcgc aacctcaacc cgcgctggga agcggacacc accctcccgg
     6061 ccgggaccag cctcaacgcc actgcccgca tcgcctggct gtaccgcttc aactgccgtg
     6121 gcggcaagcg cgcggagctt gcgaagcagt tggtggccag caacgtgcag tcggcgctgg
     6181 tacgggtcgg cccgatgacc aatcccggcg atgccaccgc aagcgcgccg acgcaggacg
     6241 cagcgccgcc gcccaggccg gtgaaaccgg ccaagccgaa gacccacaag gtgcagcgcg
     6301 gcgagaccct gagcagcatc gcccgcaagt tcgactgcga cctggccggc attgcaaagg
     6361 cgaacaagtt gaaggcgccg aagtatgcga tccgccccgg gcagcgcctg accctggccg
     6421 gatgcggggg atgaaagcgg cctgcaggcg cgtcgtcgct tccggtgacg gttgcttcga
     6481 cgtgctcgct gccggcgcgt gcggccgggt ggcgtgcagg tgagcgcgct ggaccatcgc
     6541 atcccgcccc cgctggtcgg gctggcatgc gggctgctgg cgtggctgct ggcgcgcgcc
     6601 agccccggcc tcggcgtgga ctggctgccg cgggtcccgg tcgctgcggc gctggtggcg
     6661 ctggggctgt cgctggatgt ctggggcctg gtcgcattcc gccgcgcccg caccaccctc
     6721 aacccgctgt caccggagcg caccagcgcg gtggtacaag gcgggccgta ccgcttcacc
     6781 cgcaacccga tgtacctggg catggcgtgc gtgctgctgg gcgcgtgcgc gtggctggcg
     6841 aacccgctgg cgctgctggc ggtcttcgtg ttcgtcggct acatcacgcg cttccagatc
     6901 atgccggagg agcgggcgtt gctcggcaag ttcggcggcg cgtacgcgga ctacctgcgg
     6961 cgggtcaggc gctggctgtg agtgccgcct tggccgcaac ccgcggccgc cacgttggcg
     7021 cctaggcgcg tgtcgccagc cgctgtccca gccggaccgg cgtttccgcg gcgagtcccg
     7081 cggccagcat ggccgcgccc ctgggcaaca gcacgatcac cgtgctgccg tagttgaagc
     7141 gcgccatttc ggcgaagcgc tcaagcgcga tgcccttgcc gcggtaatcc ttgcgggtga
     7201 tcgcgtcacc gtaggcgggg atctcgacgc cgctccacac ggtttcgacg ccggacacca
     7261 gcagggcgcc gaccatcacc tgcaccatcg gcccgaactc ggtgtcgaag tggcagacca
     7321 ggcgctcgtt gcgggcgaac acgcgcggca cgctggccac cgcatcggtg ccgaccgaga
     7381 acagccgccc gggcacgtgc acggtttcgc gcagggtgcc tgcccatggc atgtgcacgc
     7441 ggtggtagtc gcgcggggac aggtacacgg tggcgaacac gccatcggcg aagggggcgg
     7501 catcggcctc gctgcccagc agttcggcgg cggtgtaggc ctggcccttg gcctggaaga
     7561 tgcggccatc gacgatgtca ccgcattggc tgacgtggcc atcggccggc atcagcagtg
     7621 cgcgcggatc gggatcggcc acgcgtgcgc cgggcttgag ggcgcgggtg aagaaggcat
     7681 tgaaggtcgg gtaggcgcgc ggatcgggtt cggccgcttc cgacaggtcg acgccgaact
     7741 tgcgggtcac ggtatcgatc agccactgct tgagcgaggg gttcgtcgaa tacgccagcg
     7801 cccgcgccag cgaagacagc gcgcggtgcg gcagcacgta ggtgagcgcg gtcagcgggc
     7861 tcatcgaacg gccttggtca ggctcgccat gtcggcagcg atcgacgtga cgtccagtgg
     7921 ttgctgggcc accccggcca tccgcgcctg cggatcgagc acgaccaggc cggcggagtg
     7981 gtcgatgctg tagtcgcccg gatcgccgct ggggccgggc gccttcatga acaccagcga
     8041 cagcgagcgt gcgaatgctt ccagttgcgg gaccggcgcg gtggcggcca gggtgtcggg
     8101 atggaaccca tgcgcgtacg tgccggtgag ctcggcgctg tcgcgttccg gatcggcgga
     8161 cacgaacagc acccgcggac gggtggccgc cggcagcgag gcccattgct tctgcgcgcc
     8221 cgcgagttgg gccagggtgg tcgggcacac gtcggggcaa tgggtgaagc ccaggaacac
     8281 cagggtccag tggcccttga gctcgtccgc ggtcagcggg gtgccgtccg actgctgcag
     8341 ggcgaacgcc ggcaggctgc gcggttccgg cagcagggtc atgctgcgca ccgccggcac
     8401 gctgctcccg cgcccggcga aatggcgctg cgaggcccac aggccaagcc cggcggcaag
     8461 ggtggcgatg accagcagga ccagggcgcg tttcgacatg agcgattgga atggcggcgg
     8521 gacggccatg atagcgggcc gccgccgcta tacttcacgg cctggactcc agcccggagt
     8581 gcgcttgcgg ccaccatgtc cgaagaactg cagacgatca tcgacctgat ccgctacggc
     8641 gccagccgtt tcaacgcggc cgggctgacc ttcggccaca gctacgacaa cgcgctcgac
     8701 gaggcgaccc agctcaccct gcacgcgctg cacctgccgc acgacctgtc gccggtgtac
     8761 ggcaacgccc gggtcaccga ggccgagaag gaagacgtgc tggggttgtt cctgcgccgg
     8821 atcgaggaac gcatcccggc cgcctacctc accggcgagg cctggttcgc cggcctcagc
     8881 ttcaagtccg acccgcgtgc gctggtgccg cgctcgccga tcgcggagat gatcgaagcc
     8941 ggcttccagc cgtggctggg cgaccgcgag gtgcgtcgcg cgctggacct gtgcaccggg
     9001 tcgggctgca tcgcgatcgc gatggcgcac tacaacccgg agtggcatgt cgatggcgtg
     9061 gacatcagcg aggatgcgct ggcgctgtcg ggcgagaacg aacggcggct gcaggtcgaa
     9121 aacgtccgct tcctgaagtc ggacctgttc tcgggcctga ccggcgagca ctacgacctg
     9181 atcgtgacca acccgccata cgtcaccaac gacgagaccg acgccctgcc gcgcgagtat
     9241 tcgcacgagc cggagctcgg cctgcgcgcc ggcgacgacg gcctcgacct ggccctgcgg
     9301 atcatgcgcg atgcgccgct gcacctggac cgcgacggcc tgctggtgtg cgaggtcggc
     9361 gaggccgaac gcgcgctggt caagctgctg ccggaactgc cgctggcgtg ggtggagttc
     9421 aaggtcgggc agatgggcat cttcgtcgcc gaatgcgcgg acctgcttgc ccaccatgcg
     9481 cacatcgcca ggctggcgga cgcgcgcgag gcgcggcggc agccgcccgg cgacctgttc
     9541 tgatgccccg cgccggcatg cccgcgcccg catgaacagc tttggccacc tgctgcgcgt
     9601 caccaccttc ggcgaatccc atgggccggc gatcggctgc gtgatcgacg gttgcccgcc
     9661 gggcatcgcg atcgcacccg aagacttcgt gcaggacctc gcgcggcgcg cgaccggcac
     9721 ctcgcggcac acctcgcagc ggcacgaggc ggacgcggtg gaaatcctca gcggcgtgta
     9781 cgaggggctg accaccggca ccccgatcgc gctgctggtc cgcaacacgg atgcgcgcag
     9841 caaggactac agccgcatcg gcgagcagtt ccgcccgggc cacgcggact acacctactg
     9901 gcacaagtac ggcatccgcg acccgcgcgg tggcggccgc agcagcgcgc gcgagaccac
     9961 catgcgcgtc gccgccgcag tgattgcgaa aaagtggctc gcggatcggt acggggtgcg
    10021 ggtgcagggc tggctgtcgc agatcggcga catcgtgccg gccgggttcg atgcgggcgt
    10081 catcgaaacc aatccgttct tctggccgca tgcgccgcaa gtcggcgaac tcgagagcta
    10141 catggatgcg ttgcgcaagt ccggcgattc ggtcggtgca cgcgtcaccg tggtcgccga
    10201 tggcgtgccg ccgggctggg gcgaaccgat ctacggcaag ctcgacggcg agctggcggc
    10261 ggcgatgatg tcgatcaacg cggtcaaggg cgtggagatc ggcgccggct tcgcctcggt
    10321 gtcgcagaag ggcagcgaac accgcgacga agccacccgc gacggcttcg cgtcgaacca
    10381 tgcgggcggc atcctcggcg gcatcagcag cggccagcag gtgctggtgt cggtggcgtt
    10441 caagccgacc tccagcctgc gtttgccggg tcgcgggatg gacgtgcatg gcgaggagac
    10501 cgagatcgtc accaccggtc gccacgatcc ctgcgtcggc atccgcgcca ccccgatctg
    10561 cgaggcgatg ctggcgctgg tgctgatgga ccaggcgctg cggcatcgcg cgcaatgcgg
    10621 cgacgtgggc gcagtttcgc cgcggattcc ggcgggcatg gacccaaaac ggtagcggcc
    10681 agtcgcccca tccgccccca cgggcaacgg ctgagccgca gcggcattgc aggcacaaac
    10741 aatcaaccca gcaggagcgg cgcaagccgc gaccggttcc cgcgacaggt ggtcgcggct
    10801 tgcgccgctc ctgcaacaac cagggcagga cccaggcaat gagcaagcaa ttcaaggtcg
    10861 ccgtggtcgg cgccaccggc gcggtgggcg aggtgatgct gtcgatcctg gccgagcgcg
    10921 atttcccggc cagcgaggtg gtggcactgg ccagcgaacg ttccgccggc agccgggtgg
    10981 cgttcggcaa cggtgagatc gtggtgcagg acctggccac gttcgatccg gccgggatcg
    11041 acatcgcgct gttttcggcc ggtggatcga cgtccaggga atacgcgccg aagttcgccg
    11101 ccgccggcgc ggtggtcatc gacaattcct cggccttccg ctacgacgac gacgtgccgc
    11161 tggtggtgtc ggaggtgaac cctgggcagg tggcgaaccg cccgcgcggg atcatcgcca
    11221 acccgaactg ctcgaccatg cagatgctgg tggcgctgaa gccgctgcac gatgcggccg
    11281 ggatccagcg catcaacgtg gccacctacc agtcggtgtc cggtggtggc cgttcggcgc
    11341 tggaggagct cggccgccag accggcgcgc tgctgaattt ccaggatccc gacccgcagc
    11401 gcttcccggt gcagatcgcg ttcaacctga tcccgcacat cgacgacttc ctcgacaacg
    11461 gctacaccaa ggaagagatg aagctggtgt gggagacccg caagatcctc ggcgacgaaa
    11521 gcatccaggt gaatcccacc gcggtgcggg tgccggtgtt cttcggccac tccgaggcgg
    11581 tcaacatcga gacccgcgac aagctgaccg ccgcgcaggc gcgcgagctg ctggaacgcg
    11641 cgccgggcgt ggaagtcgtg gatgagcgcg gccccggcgg ctacccgacc ccggtcaccc
    11701 atgcgtcggg caccgatccg gtgtacgtgg gccgcatccg cgaggatctg tcgcatccgc
    11761 gcgggctgaa cctgtggatc gtgtccgaca acatccgcaa gggcgccgcc ctgaacgcgg
    11821 tgcagattgc ggaactcgtt gccagcgaag gctgagcacg gctagagtcg ctgcggggaa
    11881 ggacgtcggg agcgcgcgtg atcggcaaat tgcgaatggc agtgttgacc gggctgctgc
    11941 tggcggcggg caatgccgcg gcgctgggcc tcgggcagat cgaggtcaag tcgcggctca
    12001 accagccgct gctggccgag atcccgatca tctccaccac cccgggcgag ctgcaggcgc
    12061 tgcaggcgcg cctggcctcg ccggagacgt tccgccggat tggcctggcg gcgcccaccg
    12121 gcgcggccgc caacctgcag ttcagcctcg gcagcgacgc gcgcggacga ccggtgatcc
    12181 gggtgaccac ggtcgcgccg gtcgaccagg cggtgctgaa cttcctgatc gaagtggact
    12241 ggggccaggg ccggctggtg cgcgaatatt cggcgctggt cgatgccccg cgcaccgcca
    12301 gcgcgcccgt gcagccggcg atcagcgccc cggtggtcgc ggcgccgaac gtggtgcagc
    12361 ggccggacac gccgccggtg gccgcactgc cggtgccggt gccgccgcca gcgccgccgc
    12421 cgccgccgcc gccgccgccg gcacctccgg cacctccggc acagccagcg gtcgagccgg
    12481 aaccggttgc ggtcgcggtg gaaccgttgc cgccgccggt ggcggtggtg cccgcgccgc
    12541 cgccagcacg ggtgctgcca cccgcgccgg cccgcagcgt ggccgtggca gagccgccgc
    12601 cggcacccgc gccggcagcg gccaacgggc gctacggacc ggtgaagcag ggcgagaccc
    12661 tttcgaagat cgcgggcgcg ctcgacgttg cggccggcta cagcctcgac cagaccatgc
    12721 tggcgctgct gcgggccaat cccgacgcgt tccttggcga cgacatcaac cggctgaagc
    12781 gcggcgcggt gctgcgcgtg cccgagcccg gcgagctcgg cagcatcagt cccgatgaag
    12841 ccgcgctggt ggtgcgcgag cagatgcagc aatggcgaca ggcacggcgg ccggtcgccc
    12901 agccggacac cgcggtggcg gtcgctgaag cgccgtcgcg aaccgcggcg gctgccgcga
    12961 agcccgcgga ggcaaagtcc acggctgcga aaccgctggc ggcgaaaccc gcggaagcaa
    13021 ggcccgccgt ggccaagccg gcggcacccg cagcgcagcg ccggcagcag gcacggctcg
    13081 aaatcgtgcc gccatcggca cccggcaagg ccacaggaac acgatccggc accaacgccg
    13141 ggggcgaggg cagcatgctc cagcaacaat tgcggcaaaa agacgaagac atcgccgcca
    13201 aggccgccga gatcggcgac ctcaaggaac aggtggccga actggagaag atccgccagc
    13261 agcaggagca gttgctgtcg ctgaaggaca gcgagctggc cgcggcgcag cagcgcctgg
    13321 ccgccgcgcg tgcggccgcc gccgcaaccc ccgcgatgcc cactgccgcg gcgacccagg
    13381 ccacccaggc ctcgcagtcc acgccagcgg cggaaccgcc gcccgcgaat tcgatgccat
    13441 ggctgtgggg cgggctcgcc ctggtcggca tggccctgct cgcgtggctg ttcgtgcgcc
    13501 gccagcccaa gcgccagccg aaggcaggcg cacgccgcgg gttcgacagc gaggcactgg
    13561 cggcgagcat ggtggtcccg accgtcgaag atgtgccgcg tgataccggc atcgatgatg
    13621 cggaggacgc ggccacgtcc gtgaccaccg aggtcgacct cgaccaggta ccggctgccg
    13681 ccggcacccg ggtagagacc ccgaactggc attccggctg ggtcaaggcc gaccccccag
    13741 caccggcgcc ggcgccggag gcggagccgc ccaccccgcg cttcgtgccg caggagtcgg
    13801 cgccgccgcc gccgcaggcc agcccggaac agcggatgaa gctggcccgc gccttcctcg
    13861 acatcggtga cgatcattcg gcccgggaac tgctgcgcga gctgctggac ggcccggatc
    13921 cggccgcgcg cagcgaggcg gcgcgcctgc tgcgcgagct cggctgaggc ctgcgcaagg
    13981 gcaggcatgc gtctcgcgct cggggtcgaa tacgacggca gcggcttttc cggctggcag
    14041 cgcctgagcg cggcgggttc gcgcgagcct gacggcagcc tgcagaccgc gctggaagcc
    14101 gcgctgtcac gggtcgccga tgcgcgcatc gacaccatct gcgccggccg caccgatgcc
    14161 ggcgtgcatg gccagtgcca ggtcgtgcat ttcgacagcg acgtcgtgcg cgacgcgcgg
    14221 ggctgggtgc tgggcaccac cgccaacctg ccgccgacca tggcggtgcg ctggtgcgtg
    14281 ccggtggccg gcgatttcag tgcgcgcttt tcggcgcggg cgcgccgcta ccgctaccgc
    14341 atcctcaatc gcagcacccg gcccgggctg cagcggcagt acctcggctg ggaacggatg
    14401 ccgctggacg cacaggcgat gcacgcggcc gcgcaggcgc tggtcggcga acgcgatttc
    14461 tccgccttcc gcagcgtgca gtgccaggcc acccatgcgc gccgcgacct gcaggagatc
    14521 agcgtgcacc gccgcggcgc ggaagtggtg gtggaagtcc aggcgaatgc cttcctgcac
    14581 cacatggtgc gcaacatcgt cggctcgttg ctggtggtgg ggcgcggcga acggccgccc
    14641 gcttggatcg ccgagttgct ggagcggcgc gaccgcaccg ttgccggccc caccgcgccg
    14701 gcggccgggc tgttgttcct cggtccgcgc tatccgcgcg ggtgcggctt gccggacgag
    14761 gtgtcgctgt gagcggggcg cacacccgga tcaagctgtg cggcatgacc cgcgccgagg
    14821 acgtggagct ggcggtcgcg ctgggggtgg actacatcgg cctggtcttc gccggtggca
    14881 gcccgcgcca tctcgcgctt gcgcaggcgc agcccttgcg cgcgctggtg ccggccagtg
    14941 ttggcgtggt ggcgctggtg atggatgcgc cgcgcgcgga ggtcgaggcg atcgcctcca
    15001 cgctgcggcc ggactgcctg caattccacg gtagcgagga agaccggttc tgcgccagct
    15061 tcggcatgcc gtactggaag gcgatcgcga tgggcgacgg cgccgacccg gcgctggcga
    15121 tggcacgttt cccgtccgcg cgcggcttcg tgctggacgg ccacggcgcc ggcgaggccg
    15181 gcggcagcgg tcgcagtttc gactggaccc gctggccgcg cgaccagcac cggccggtcc
    15241 tgcttgccgg cgggctgtcg gcgcagaacg tcgcactggc gctgcgcacc gcgcggccgt
    15301 ggggcgtcga tgtgtccagc ggcatcgaac aggcgccggg ggccaaggac gcggcgcaca
    15361 tgcgcgcgtt cgtggccgcg gtacgcgccc acgacgctgc ctcctgagcc gcggcaagcg
    15421 gcccggttcg ggtaccctat gcaggccatg aacgcctcca atcgcccgcc ttcgccgttc
    15481 ggtcgctcgc tgcgccggtg gcgccgggtg cccgcggccg cggttcgata acgccaccgg
    15541 tgtgtccagg ccggcaacgg cctgcaaccg ggttcgcgaa cgtgcatcca tgccgtcgcc
    15601 tgcccaggcc ccccgattcc ggagaccccc gcgtgaacac ccctgcaccc agcactgcgc
    15661 ccgccgtggc gatcgatttc aaccagtacc cggacgcctc cggccacttc ggccgctacg
    15721 gcgggcgctt cgtcgccgag accctgatgg gcccgctgga cgaactggcg gccgcctacg
    15781 acgcggcgaa ggtcgatccg gccttcatcg ccgagttcga ggccgacctc gcccattacg
    15841 tcggccgccc cagcccgatc taccacgccg aacgcctgag ccgggaagcc ggcggcgccc
    15901 gcatcctgct caagcgcgaa gacctgaacc acaccggcgc gcacaagatc aacaacacca
    15961 tcggccaggc gctgctggcg agcaggatgg gcaagacccg gatcatcgcc gagaccggcg
    16021 ccggccagca cggcgtggcc tcggccacgg tcgccgcgcg gctgggcctg gagtgcgtgg
    16081 tctacatggg cgccaccgac atccagcgtc agaagatcaa cgtctaccgg atgcagttgc
    16141 tgggggcgac ggtggtgccg gtgaccagcg gctcggcgac cctgaaggac gcgctgaacg
    16201 aggcgatgcg cgactgggtc agcaacgtgc gcgacacctt ctacatcatc ggcacggtgg
    16261 ccggcccgga cccgtatccg cggatggtgc gcgacttcaa cgcggtggtc gggcgcgagg
    16321 cgcgcgcgca gatgctggcc gaatacggcc gcctgccgga cgtggtcacc gcctgcgtcg
    16381 gcggcggcag caatgcgatc gggatcttcc atgcgttcct caacgacgcg ggcgtgcgca
    16441 tcgtcggcgc cgaggcggcg ggcgagggca tcgccagcgg ccatcacgcg gcatcgctgg
    16501 ccgccgggcg gcccggcgtg ctgcacggca accgcaccta cgtgatctgc gacgacgacg
    16561 gccagatcgt cgagacccat tcggtgtccg ccgggctcga ctaccccggc gtcggtccgg
    16621 agcatgcgtt cctgaaggac agccgccgcg ccgactacgt gggcgtgacc gacgacgagg
    16681 cgatggcggc cttccacctg ctggcgagga ccgagggcat cctgccggcg ctggagtcca
    16741 gccatgccat cgcccaggcg atcaagctgg cacgcgagct gccgaaagac gggatcgtgc
    16801 tctgcaacct ctccgggcgc ggcgacaagg acgtgcacac catcgccggc cgcgaaggcc
    16861 tggcgcttta gcctgcagtt ggcctgcggc ccggcttcgg cgacaatagc cggttcattc
    16921 aatgcgatca ggagtccggc catggccgat gaacaagccc agcaggaggc gctggcccag
    16981 cccgcggccg ccgacaacca gctcgacccg gcattcttca ccgcggtcaa cgaatacctc
    17041 gagttgacca acaagcagtc gcgccagcat ggcctgaagc gcatcagcct ggccagcctg
    17101 tatgcggccg cgcgcttcaa ctcgcacgtg ttcctgtcca acgtcgcccc gggccaggcc
    17161 gccggtgacc gcgcccactt cctcgactac atggccacca tgtaccgccg catgctcaac
    17221 gagcacctcg atggcctcgg ccatgagcgc ggcatcgatg tcggcgagtc cgagcttgcc
    17281 gccgaatacg ccgccgccgg cgtgcaggtg ggccgcatgg gccagacccc cgccggcgac
    17341 tatgttgccg ctgccggcgc gaccccgccg gccgcgtccg acgaatgagc ctgccgcggc
    17401 acgcgcgcct gcaggcgcgg ctggacgcac tggaatcggc cggccgcaag gcgctggtgc
    17461 cgttcgtcac tgccggcgat ccctcgctgg cggccacggt gccggtcatg cacgcgctgg
    17521 tcgaggccgg cgccgacgtg ctcgagctgg gggtgccgtt ctccgacccg atggcggacg
    17581 gaccggtcat ccagcgcagc tcggagcgtg cgctggcccg cggcgccggc atccgctacg
    17641 tgctggagtg cgtgcgcggt ttccgcgagc gcgaccaggc cacgccggtg gtgctgatgg
    17701 gctacctcaa cccggtcgag atccgtggcc cggcgagctt cgccgcggcc gccgccgatg
    17761 ccggcgtgga cggcgtgctg ctggtcgacc tgccgccgga agaggccgac gaataccgcg
    17821 ccgcgttcgc gctgaacgga ctggcgctga tctcgctggc atcgccgacg acgcccgagg
    17881 cgcgcctgca acgcctggtc acccaggccg acggttacct gtactacgtc agcttcgccg
    17941 gcgtgaccgg tgccgaccgg ctcgatgtcg gcgatgccgg ccagcgcctg cagggcctgc
    18001 gcacgatggc cgcgatcccg gtgtatgcgg gcttcggcat ccgcgatgcg cacagcgcgg
    18061 cggcgatggc tgcgcacgcc gacggcgtgg tggtcggcag tgcgctggtg tcggcactgg
    18121 acggcagcct cgacgaggcc gaggccgcgc agcgtgccgt cgccttcctg cgcccgctgc
    18181 ggcaggcgct ggacggcctg gccgtggctg cctgagctag acttccccgg ttgtcatccc
    18241 ggcgcgagcc ggggtccggc cctccggtcg tgtcgcaggg ccggatgcca ggttccggcg
    18301 cgtgccggca tgaccgacac ctgtcccgat caaccgcggt gatcgccccg aatgagctgg
    18361 cttaaaaagt tgatgcccgc ccgcatccgc accgataccg gcgtggaccg caagcgcagc
    18421 gtgccggaag gcctgtggga aaaatgcgag cgctgcggcg cggtgctgta ccggcccgaa
    18481 ctcgaggaac acctcgaggt ctgcccgaag tgcagcttcc acatgccgat ccgcgcgcgc
    18541 gccaggctcg cggcgctgct cgacgacggc atgggccacg agctcggcgc cgcgctcggc
    18601 ccgaccgacg tgctcaagtt ccgcgaccag aagaagtatg cggaccgcat caaggccgcg
    18661 cagaagtcca ccggcgagcg cgacgcgctg gtcgcgatgg agggccggct gaagggccgc
    18721 gagctggtcg cggccgcgtt cgacttcgcc tacatgggtg gctcgatggg gtcggtggtc
    18781 ggcgagcgct tcaccctggc cgcggagcgt gcgctggaat tgcgctgtcc gttcgtgtgc
    18841 ttctccgcca gcggcggcgc gcgcatgcag gagagcctgt tctcgctgat gcagatggcg
    18901 aagacctcgg ccgcgcttgg ccgcctgcgc gcggcggggc tgccgtacat ctcggtgctg
    18961 acccatccga ccaccggcgg cgtgtccgcc agtttcgcca tgcttggcga cctcaacatc
    19021 gccgagccgg aggcgctgat cggcttcgcc ggccagcgcg tgatcgagca gaccgtgcgc
    19081 gagaagctgc ccgagggctt ccagcgcagc gagttcctgc tcgcgcacgg caccgtcgac
    19141 cagatcgtcg accgccgcga gatgcgcgaa cggctggccc acgtgctggc gatcctgatg
    19201 aagcagccgg cacccgccga ggacgcggag gcggcatgag cgcacgccgg tatttcggca
    19261 ccgatggcat ccgcggccgc gtcggcgatg gcccgatctc ggccgacttc gtgctgcgcc
    19321 tgggcaatgc ctacggccac gcgctgaccg ccgccgcggc aggcggcgac cggatctggc
    19381 gcaagccgat ggtggtgatc ggcaaggaca cccggatctc caactacatg ttcgaggccg
    19441 cgctggaagc cgggctggtc gccgccgggg tggacgtgca gctgatgggg ccgatgccga
    19501 cgcccgcggt ggcgcacctc acccattcga tgcgcgccga tggcggcatc gtgatctcgg
    19561 cctcgcacaa cccgcaccac gacaacggca tcaagttctt ctcggcgcag ggtgaaaagc
    19621 tggacgacgc caccgagctg gcgatcgagg ccgcgctgga cgcgccgttc cgcacggtgc
    19681 cgtccgggca gctgggcaag gcggtgcgca cccgcgacac catcggccgc tacgtcgagg
    19741 cctgcaagaa ttcggtgccg cgcggcttca acctcggcgg catgcggatc gtgctggatt
    19801 gcgccaacgg cgcgacctac cagcttggcc cgctggtgct gcgcgaactc ggcgcgcggg
    19861 tcgacgcgat gggcgtcgag ccgaacgggg tcaacatcaa cgacggggtc ggttccaccc
    19921 atccggaagg cctggccgcg cgcgtgcgcg aaaccggcgc ggatatcggc atcgccttcg
    19981 acggcgacgg cgaccgcgtg ctgttcgtcg acgccgccgg caacgtgcgc gacggcgacg
    20041 acctgctgta cgtactggcc tgcgactggc aggaaagcgg ccgcctgcgc ggaccggtgg
    20101 tcggcaccct gatgaccaac tacggtttcg agcaggcgct ggccgcgcgc gggatcggct
    20161 tcgtccgtgc aaaggtcggc gaccgccacg tgcaccagca actgctggcg aacgacgcgg
    20221 tgctcggcgg cgaggcttcc ggccacctgc tgtgcctgga ccgcgccagc accggcgacg
    20281 gcatcgtcag cgcactgcag gtgctggaag tgctgcggcg ccgcggcgtc ggcctggccg
    20341 aggcattgca ggggttgcgc aaggtgccgc agaaaaccat caacgtacgc tgcgagggcg
    20401 gccgcaagcc ggccgatgca gacagcgtga aggccgcgct ggccgcggcg cagggttcga
    20461 tcgagggccg cggccgcgcc ttcctgcgcg cctccggcac cgagccggtg gtgcgtgtca
    20521 ccgtcgaggc ggacgatgcc gcgctggtcg atgccaccct cgccgtgctt gcggatgcgg
    20581 tgcgcgcggc ggcggcctga cccggaccga aaccattgcc atgcggcggg tgcacgcgcc
    20641 cgcccggcga accgaagaca ccgacatgag cagccagtcc atttcgccgc gaatcccgac
    20701 cctcgacatc cgccgtttca ccgatccggc gtcgccgcag gaccgccagg ccttcatcga
    20761 cgagctgggc gcggcctacc gcgagtgggg tttcgccggg atccgcaacc acggcatccc
    20821 gcaggcacgg atcgacgagg cctacgacac tttccgcagg ttcttcgcgc tgccggaggc
    20881 gaccaagcgc cagtaccacg tggcgggcgg cggcggcgcg cgcggctaca caccgttcgg
    20941 ggtcgagacc gcgaagggcg cgcagtactc cgacctcaag gagttctggc acatcggccg
    21001 cgagatcccg cgcgattcga agtacgcaaa cgacatgccg gcaaacgtgt ggccgcagga
    21061 catgccggag ttccaatcgg tcggctacgg cctgtaccag tcgctggatg cgctgggttc
    21121 gcaggtgctg cgcgcgctgg cgctgcacat cggcctgccg gaagactatt tcgccgacaa
    21181 gaccgattcg ggcaactcga tcctgcgccc gatccactac ccgccgatca ccgtcgacga
    21241 catccccaac gtgcgtgcag gcgcgcacga ggacatcaac ctggtcaccc tgctggtggg
    21301 cgcaagcgcg gaagggctgg aagtgctgtc gcgcaagggc gagtgggtgc cgttcaccgc
    21361 ggatgccgac accatcgtgg tcaacatcgg cgacatgctg cagcggctga ccaaccacgt
    21421 gtatccgtcc accacccatc gcgtggtcaa tccgccgggc gagaaggcgc ggcagccgcg
    21481 ctactccacc ccgttcttcc tgcacccgaa cccggatttc ctgatcgacg tgctgccgtc
    21541 ctgcgtcagc gcggacaatc cgagccgcta tccgcagccg atcaccgcgc agggctacct
    21601 ggaagagcgc ctgcgcgaaa tcaagctgaa gtgacccatc catccatcga ggaaccacgc
    21661 atgcgccgca ggatcgtcat cgggaactgg aagctgcacg gcgaccgcgc gttcgcgcac
    21721 cgcctggtcg acgcgatcgc cgcggtgccg gcggccgcgg gggtcgagcg ggtgatcctg
    21781 ccgccgctgc cgtacctcgg cgaacttgtc gcgcactgcg ccgggaccga cctgcgcttc
    21841 ggcgcccagg acgtgagcgc caacgtgcag ggcgcctaca ccggcgaggt ctcggcctcg
    21901 atgctggtcg acatcggcgc ggtgtacggg ttggtcgggc attccgagcg ccgccagcac
    21961 catcgcgaga tcagcgaact ggtggcgagg aagttcgtcg ccgcgaagga cgcgggcctg
    22021 accccgatcc tgtgcgtggg cgagtccctg cacgagcgcg aggccgggca gaccgaatgg
    22081 cggatcgagg agcagctgca ggcggtgttc gacctggccg gcccgcaggc cttcgccggc
    22141 gcgatcatcg cctacgagcc ggtgtgggcg atcggcaccg gccgcaccgc cagcccggcg
    22201 caggcacagg cggtgcacgc cttcatccgt ggcgaggtcg ccgcccgcga tgctacaatc
    22261 gccggctcgc tgccgatcct gtacggcggt agcgtgaagc ccgacaacgc ggccagcctg
    22321 ttcgcccagg ccgatgtgga cggcggactg atcggcgggg cctcgctggt cgccgcggat
    22381 ttcctggcca tcgcgtccgc cgcggcggcc tgacccaaga cggataccga accacgatgt
    22441 tgctgcaact catcaatgtg ctttacgtgc tggtcgccat cgcgatgatc ggcctgatcc
    22501 tgatgcagcg cggcgccggt gcgcaggcgg gctcgggctt cggcggcggg gcttccgcca
    22561 ccgtgttcgg ttcccgcggt gcctcgaact tcctgaccaa gagcaccaag tggctggcga
    22621 tcgtgttctt caccatcagc atgggcatgg cggtctacgc cagcaagagc gcccgccagg
    22681 gcggtgccga accggccgcc gcggaccttg gggtgatggg ccaggctgcc ccggcggcac
    22741 cagccaggca ggtgccgagc gcgcaggt
//
LOCUS       DAORUK010000018        21902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_1972_length_21902_cov_22.266032, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000018 DAORUK010000000
VERSION     DAORUK010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 21902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21902
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1972_length_21902_cov_22.266032"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /gene="rpsK"
                     /locus_tag="PK818_02305"
     CDS             <1..224
                     /gene="rpsK"
                     /locus_tag="PK818_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HQY81600.1"
                     /translation="KSTPFAAQVAAEKAGKAALDYGVKSLEVRIKGPGPGRESAVRSL
                     NNVGYKIINIIDVTPIPHNGCRPPKKRRV"
     gene            244..876
                     /gene="rpsD"
                     /locus_tag="PK818_02310"
     CDS             244..876
                     /gene="rpsD"
                     /locus_tag="PK818_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004145446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HQY81601.1"
                     /translation="MARYIGPTCKLARREGADLGLKSPARALDSKCKLEQKPGQHGAT
                     MTRKGKLSDYATQLREKQKVKRIYGLLERQFRNYYKKAATKKGNTGENLLQMLETRLD
                     NVVYRMGFAVTRPAARQLVSHRGITVNGKSVNLPSYAVKAGDAIALSEKAARQLRVKE
                     SLTVAQQMDLSPSWVDVDATKFAGVFKAVPARGDLPSDINEALIVELYSK"
     gene            929..1927
                     /gene="rpoA"
                     /locus_tag="PK818_02315"
     CDS             929..1927
                     /gene="rpoA"
                     /locus_tag="PK818_02315"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020852431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HQY81602.1"
                     /translation="MTVTATQVLRPRGPQIERIADNRAKVVIEPLERGYGHTLGNALR
                     RVLLSSIPGFAITEVEIDGVLHEYTTVEGLQEDVLEVLLNLKDVAIRMHSGDSDTLEL
                     KKQGPGIVTAGDIKTSHNVEVLNPDHVIASLTKDVALNMRLTISRGFGYQPAAARRRP
                     DEETRAIGRLMLDASFSPVRRVAYAVESARVEQRTDLDKLVLDIETNGTIDAEEAVRT
                     AADILSDQLSVFGDFTHRDRGAPKQAASGVDPVLLRPIDDLELTVRSANCLKAESIYY
                     IGDLIQKTEVELLKTPNLGKKSLTEIKEVLAQRGLSLGMKLENWPPAGISAHGMLG"
     gene            2211..2582
                     /gene="rplQ"
                     /locus_tag="PK818_02320"
     CDS             2211..2582
                     /gene="rplQ"
                     /locus_tag="PK818_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HQY81603.1"
                     /translation="MRHQKSGRKFNRTSAHRGAMFSNMAASLFKNELIRTTLPKAKEL
                     RRVAEPLITLAKVDGVANRRLAFARLRDKEAVGTLFTILGPRYAQRPGGYLRILKCGF
                     RAGDNAPMAYVELVDRPQATE"
     gene            2685..3206
                     /locus_tag="PK818_02325"
     CDS             2685..3206
                     /locus_tag="PK818_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HQY81604.1"
                     /translation="MNPFRSLSFRAQFLLGLAACVALLGYAFYVQFQLGIQPCPFCIF
                     QRLCFAALGMVFLLGALHDPRAPGARKAWSVLAVIAAGAGAGYAGRHSWVQLNPPELP
                     SCGPGLNFIVEQHSWLGAAREVLLATGDCSNIDWTFLGLSMPMWVLAWFVGLGLWALH
                     AGFRRRGKHRFHR"
     gene            3267..4655
                     /locus_tag="PK818_02330"
     CDS             3267..4655
                     /locus_tag="PK818_02330"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HQY81605.1"
                     /translation="MPHAQHAVPPADNDIDAWAPDSWRTRRALQLPQYPDLAALAAVQ
                     DELRALPPLVTSWEIQSLKQQLADAQEGSRFLLQGGDCAETFAECNSSVISNRLKVLL
                     QMSLVLVHGLKLPVVRVGRFAGQYAKPRSADTETIGDTTLPSYRGDMVNAPEFTAAAR
                     TPDPRRMVKAHARSAMTMNFVRALIDGGFADLHHPEYWDLGWVGQAPLAHEYQRMVGA
                     IGDAVRFMETLTGSQVHNINRVDFYTSHEALLLPYEEAQTRQVPRQHGWFNLGTHYPW
                     IGMRTAAMDGAHVEYFRGIRNPVAIKIGPSVAPDQLLRLVDVLNPDDEPGKLAFIHRM
                     GATAIADKLPPLLEAIRREGRRVLWVCDPMHGNTEATANGYKTRRFDNIRGELEQAFD
                     VHAAAGTRLGGVHLELTGENVTECLGGARELTELDLQRDYRSTVDPRLNYEQALEIAM
                     LIVRKRGEIAAD"
     gene            complement(4695..6305)
                     /locus_tag="PK818_02335"
     CDS             complement(4695..6305)
                     /locus_tag="PK818_02335"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017112221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HQY81606.1"
                     /translation="MTRLSLAIALLLLAACQQTDTPQPASAEAAATAAPAPFAFEEAS
                     IASLQEQMASGSLTSRALAQAYLDRIAAIDDAGPRLNAVIEANPDALKEADALDAERT
                     SGKLRGPLHGIPVLLKDNIDATPMVNSAGSLALAEHRPKSDAFLVARLRAAGAVILGK
                     TNLSEWANIRSTRSTSGWSARGGQTRNPYALDRNPCGSSAGTGTAIAANLAAVGIGTE
                     TDGSIICPSAVAGLVGIKPTVGLVSRSGIIPISHSQDTAGPMARSVADAAAVLTAIAA
                     ADAADAATSEHPARTAIDYTAHLKPDALKGARIGVLRKAMGYQPDVDAAFERAVAALK
                     AAGATVVDAEIPTAGQWGDAELEVLLYELKADLNAYLTSSGAPVKSLAGVIAFNKQNA
                     GTEMRYFAQELFEQAEAKGPLTDREYLDARDKARRLAGPEGIDAALKKDNLDALVAPS
                     MSPAWPTDPINGDHFTGAGYGAAAVARYPSISVPMGHSHGLPLGITFMGTAWSEARLV
                     ELAHAYEQASKARTPPKFLLTVDLDEAR"
     gene            complement(6394..8238)
                     /gene="typA"
                     /locus_tag="PK818_02340"
     CDS             complement(6394..8238)
                     /gene="typA"
                     /locus_tag="PK818_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HQY81607.1"
                     /translation="MSESNSSVERLRNIAIVAHVDHGKTTLVDCLLKQSGTLNERTVL
                     AERVMDSNDQEKERGITILAKNTAITWQGNRINIVDTPGHADFGGEVERVLSMVDSVL
                     ILVDAMDGPMPQTRFVTQKAFAMGFKPIVVVNKIDRPGARPDWVIDQVFDLFDKLGAT
                     NEQLDFPIVYASALHGYASLDDSAREGDMTPLYEAIMQHVAPPQVDLDGPFQMRISQL
                     DYSNFVGLIGIGRIQRGKVRRNMPVSVVDREGKKRQGKVLQVLGFMGLERIETEEAGA
                     GDIVAISGVADLSISDTVCALDAPEALPALTVDEPTISMTFQVNNSPFAGNKDMSGGK
                     FLTSRQLRERLERETLHNVALKVEEGSDPDKFLVSGRGELHLSVLIENMRREGFELAV
                     SRPEVIIKEIDGQQMEPVEQLVVDIEEVHQGGVMEKLGTRKAALKNMESDGKGRVRLD
                     YMIPARGLIGFQNEFRTLTQGSGLLFHVFDHYGPKETGAIAKRQNGVMIANAAGTTPA
                     YSLGPLQERGKLFAAEGDNVYEGQLVGIHSKDNDLTVNAIKPKPLTNMRASGKDDAIQ
                     LSPAIKYTLEQALDFIEDDELVEVTPKEIRLRKKFLTESDRKRASRSA"
     gene            8365..8859
                     /locus_tag="PK818_02345"
     CDS             8365..8859
                     /locus_tag="PK818_02345"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY81608.1"
                     /translation="MSLIATFDTDRGPIRVELAAEKAPLTVANFVNLARRGFYDGLNF
                     HRVIPDFMVQGGCPEGSGRGGPGYRFEDETNNGVKHERGVLSMANAGPNTNGSQFFIT
                     HIKTDWLDGKHTVFGKVIEGLDVVDAVKQGDLIKSVKIEGDAEAALAARADRVAEWNK
                     ILAA"
     gene            9045..10244
                     /locus_tag="PK818_02350"
     CDS             9045..10244
                     /locus_tag="PK818_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQY81609.1"
                     /translation="MPQLARRIGRAKPSAIMQVAEKAKRLKAEGRDIISFSIGVPNFL
                     PAEHVYAAARDALARDSGQYGSNRGSDALLDAFIAHMAKIGLAGYTRANCATGIGAKH
                     VIYNLAEALLDEGDTIVFPVPYWTSYMDIADIVGARVELLPCPASQDYKMTPAQLDAA
                     LASKPRVFLFNNPSNPTGMVYNRDEIVALAEVLVKHPDTWIITDDIYNRMLFDGLEYA
                     NFVQVRPELRDRVIFIDSLSKTYGMPGWRVGFMAGPEAVAQALTTMNSNHITNIPEIV
                     TAAAIAALNGPQDVPTQKNAEFQAKRDQVMAVMDAIPGVVCPRPQGAFYVFPDISCAF
                     GKSHNGTKIGNDVDFCNALLEAKGVACVPGSAFGEPRALRISYTCPTPQLAPGLARIQ
                     EFFAELT"
     gene            10307..11290
                     /locus_tag="PK818_02355"
     CDS             10307..11290
                     /locus_tag="PK818_02355"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HQY81610.1"
                     /translation="MKAPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQMLELP
                     MEKAQAALRGVMMELEDCAFPLLAGMVGTDDAEVAFKDADYALLVGARPRGPGMERKD
                     LLLENAKIFTAQGAALNKVASRNVKVLVVGNPANTNAYIAMKSAPDLKPGNFTAMLRL
                     DHNRALSQLAIKAGVAVADIEKLVVWGNHSPTMYPDYRFATVNGESLKDKVNDAEWNA
                     NTFIPQVGKRGAAIIEARGLSSAASAANAAIDHMRDWALGTNGKWATMGVPSDGSYGI
                     PEGVIYGVPVTCANGEYTRIEGLPVDDFSRAAMDKTLAELEEERAGVAHLL"
     gene            11571..12119
                     /locus_tag="PK818_02360"
     CDS             11571..12119
                     /locus_tag="PK818_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81611.1"
                     /translation="MILRRVIQHVRKQEWTAIAIDFAIVVIGVFVGIQVANWNQELAD
                     ERLGHAYALRLQADLKRDLVARRELVDYHAAVLRGVERTDALLADPRSDAKALVVNAY
                     RASELNYRATSRATWDEIVSSADTGLLPPGVARSAGEYFAIDSARLTLDGLTQSGYRH
                     RVRMIIPHRSDRPTHLPMQARR"
     gene            12116..14005
                     /locus_tag="PK818_02365"
     CDS             12116..14005
                     /locus_tag="PK818_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81612.1"
                     /translation="MKFISELKRRNVIRMAGLYLVGAWLVTQVAATLLPVFEAPAWVM
                     KAVVAVLAVGFVPALIVAWAFELTPEGLKRDGMVPPSESIAPQTARRMDRMLLVVSAL
                     ALAYFGFDKFVLAPQRDAALVAQTTRAVVANAGARAAKQGPDPRSIAVLPFANMSADP
                     ENEFFSDGLSEEVLNSLARIDRMQVVGRTSSFQFKGKNVDLRGIGQKLGVAHILEGSV
                     RRDGERARITAQLIRASDGVHLWSQTYDRTLQDTLAVQLDIAEQVAGALGVLLDDKQR
                     GTMRKAGVGNVDAFIHYQKAWKLYLAAHGDKSVGLLGGLQLANAEFDRAIALEPNYSM
                     AHYAKADLYEHTLIDDHTTHAERADAQRAVLQTLQRAAATGPDAQQREFALAERQMLG
                     DDWRGITDQLSKALAQPGCGAPNWMPVFASAFGYADALEPVLARAATCDPLNAINWNT
                     RTWVARADGKAERIQAIYAQWRTYAPALPQAPHERLALAMLGHEDKLPPKTKKLDNLG
                     AAEFGTAMQIARLRGLNRAQAQAWTAGRRNEDANIGRQWQIVELVEAALFGDRADANR
                     RAAVLDARPAGGLLLAVAVTYCGCGAPFDLSATPNFKARLAESGLRWPPPVAIRYPPI
                     PGTAP"
     gene            14002..14754
                     /locus_tag="PK818_02370"
     CDS             14002..14754
                     /locus_tag="PK818_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81613.1"
                     /translation="MSFLAELRRRMERMFVLLLALALGLFAFDRFALAPRREAALVAA
                     TTETAKAEAGAGKQAAISEKAIAVLPFVNMSADRDNEYFSDGIAEEILNALAQVKDLK
                     VAGRTSSFQFKGRNESLTSIGQALGVANVLEGSVRKQGDKVRITAQRLPPRRGGAHRT
                     CAGDRSDGAQRDALARRALPARWQHRRRRAIPQAGQGDRPAAGGSRTWRNRPLAWRHG
                     FGATHLERLLGPVARANGAGRAKGAGHRHVRR"
     gene            14777..15073
                     /locus_tag="PK818_02375"
     CDS             14777..15073
                     /locus_tag="PK818_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81614.1"
                     /translation="MSAVEAYVASGPEFLPGIVPMWLAQIGQEARALELDRTRSEVDN
                     SDFMVYLFSPDGKSLRALPEFPAYMRAKGFPALWDKYGAPDMCRKDAAGDYRCD"
     gene            complement(15080..15595)
                     /gene="msrA"
                     /locus_tag="PK818_02380"
     CDS             complement(15080..15595)
                     /gene="msrA"
                     /locus_tag="PK818_02380"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HQY81615.1"
                     /translation="MSTSTESAILAGGCFWGMQELVRKLPGVLATRVGYSGGDVANAT
                     YRNHGTHAEAIEIVFDPARTSYRDILEFFFQIHDPTTVNRQGNDRGSSYRSAIYYASD
                     EQHRIAVDTIADVEASGLWPGKVVTELAPAGPFWEAEPEHQDYLQRYPGGYTCHFVRP
                     GWKLPRRGGAG"
     gene            complement(15592..16047)
                     /gene="msrB"
                     /locus_tag="PK818_02385"
     CDS             complement(15592..16047)
                     /gene="msrB"
                     /locus_tag="PK818_02385"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HQY81616.1"
                     /translation="MAKTYSKTPEALARLTPEQRRVTQDAGTERPFDNAFHDSKAPGL
                     YVDIVSGEPLFTSLDKFDSGCGWPSFTRPVEPAHVLERSDSSHGMRRTEVRSAHGDSH
                     LGHVFTDGPRDAGGLRYCINSAALRFIPADVLEQEGYGDYRHLFAGDRA"
     gene            16185..17069
                     /gene="prpB"
                     /locus_tag="PK818_02390"
     CDS             16185..17069
                     /gene="prpB"
                     /locus_tag="PK818_02390"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HQY81617.1"
                     /translation="MDERQSAGAQFRAALATESPLQVMGAITAYAGLMARRTGYRALY
                     LSGGGVAANSLGVPDLGISTMEDVLTDARRIVDATGMPLLVDIDTGWGGAFNIGRTIR
                     AFERIGVAAVHMEDQVGQKRCGHRPGKEVVTKEEMIDRIRAAVDARTDRDFVIMARTD
                     AAAVEGLDSAIERAVAYVEAGADMVFPEAMTSLDDYRKVKAAVQVPILANLTEFGSTP
                     FYTTDELRDAGVDIALYCCGAYRAMNKAALNFYEAVRRDGTQKHVIDTLQTRAELYDF
                     LGYHAYEDKLDALFAQNR"
     gene            17085..18242
                     /gene="prpC"
                     /locus_tag="PK818_02395"
     CDS             17085..18242
                     /gene="prpC"
                     /locus_tag="PK818_02395"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HQY81618.1"
                     /translation="MSEQSAATPGFKPKKSVALSGTAAGNTALCTVGRSGNDLHYRGY
                     DILDFANASEFEEIAYLLVHGKLPNKAELAGYKARLKSLRGIPAAVKAALEELPPSAH
                     PMDVMRTGVSVLGCVSPEKDDHNHPGARDIADKLMASLGSMLLYWYHWSHNGRAIDVE
                     TDDDSIGGHFLHLLHGEAPRESWVRAMHTSLILYAEHEFNASTFTARVIAGTGSDMHS
                     CIAGAIGALRGPKHGGANEVAFEVQKRYENPDEAEADIKARVERKEVVIGFGHPVYTV
                     SDPRNKVIKDVARELSAEQGDMKMYDIAERLESVMWDIKKMFPNLDWFSAVSYHMMGV
                     PTAMFTPLFVIARTSGWSAHVIEQRIDGKIIRPSANYTGPEDRVFVPLAQR"
     gene            18257..18733
                     /locus_tag="PK818_02400"
     CDS             18257..18733
                     /locus_tag="PK818_02400"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HQY81619.1"
                     /translation="MDWLIPAGYFAEYLLVAVVAFAVTALVVATHYEGVHALARRFAS
                     RAPRRDHLAMLKIIFALLALHIVEIWYYGLAYWGLVMIPGTGFVHGEFGMDRLFDAVY
                     LSAITYSTVGFGDLAPVGAIRLVAGLEALTGLLMITWSASFTYLEMSRLWADERRR"
     gene            complement(18746..19213)
                     /locus_tag="PK818_02405"
     CDS             complement(18746..19213)
                     /locus_tag="PK818_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004154513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HQY81620.1"
                     /translation="MKLAWILWMAGNVLPQPAADSLCLSTTVYLEARDQSVRGQQAVA
                     EVALRRRDSGLWGRNLCQVVTARKQFAPTIVSPQTRLSNREAWAEAVTIALESERNWA
                     RPAGQRREIVPGASHFMAHAIASPSWRNAYQVATIGDHTFLQVQSLRPRRSRG"
     gene            complement(19504..21528)
                     /locus_tag="PK818_02410"
     CDS             complement(19504..21528)
                     /locus_tag="PK818_02410"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HQY81621.1"
                     /translation="MNESSPYPHLFRPLDLGFTTLRNRVLMGSMHTGLEDRASDFPRL
                     AAYFAERAAGGVGLIVTGGFAPNLVGWLKPFAGRMSWPWHVNRHRQVTAAVHAHDAKI
                     CLQLLHAGRYAYHPLSVSASKLKAPINPFTPRGLSARGVERQIRDFANAAKLAREAGY
                     DGVEIMGSEGYLLNQFTVGRTNKRSDAWGGDAGKRMRFAVEIVRRVREACGPDFIIVY
                     RLSMLDLVEGGNDWDAIVAQAQAVEAAGATIINTGIGWHEARVPTIATSVPRAAFVGV
                     TAKLKPHVRLPLVATNRINMPDVAESVLASGFADMVSMARPLLADPQWVNKARAGRSP
                     AINTCIACNQACLDHVFENKPASCLVNPRACAETELNYTRTTTPKSVAVVGAGPAGLA
                     CATIAAERGHRVTLFDAAGEIGGQFNLAKTIPGKEEFHETLRYFGHRITETGVELRLH
                     TRVDAAGLSGFDEVVVATGVTARAVDFPGADHPKVVGYLDVLQGRVEPGKTVAIIGAG
                     GIGFDVAEFLVQEGGSPTLDSGRWMAEWGVDAAFESPGGLVRPQPEPAARKVWLLQRS
                     QGKPGARLGKTTGWIHRSTLKAKGVKMLGGVEYLGVDDAGLHIRVDGGEQVLEVDHVV
                     VCAGQESRRELFDALLAQGRQPRLIGGADVASELDAKRAISQGSHVAASL"
BASE COUNT         3557 a         7370 c         7463 g         3512 t
ORIGIN      
        1 gcaagtcgac cccgttcgcc gcgcaggtcg ccgccgagaa ggccggcaag gccgccctcg
       61 attacggggt gaagtcgctg gaagtgcgca tcaagggccc gggcccgggg cgcgagtcgg
      121 ccgtgcgttc gttgaacaac gtcggctaca agatcatcaa catcatcgac gtgacgccca
      181 tcccgcacaa cggatgccgt ccgccgaaga agcgtcgcgt ctgacgccgg ggagcagaag
      241 aaaatggctc gttatatcgg tcccacctgt aagctcgccc gtcgcgaagg cgccgacctc
      301 ggcctgaagt cgccggcccg cgcgctcgat tccaagtgca agctggagca aaagcccggc
      361 cagcacggcg ccaccatgac ccgcaagggc aagctgtccg actacgccac ccagctgcgc
      421 gagaagcaga aggtcaagcg catctacggc ctgctggagc gccagttccg caattactac
      481 aagaaggccg cgaccaagaa gggcaacacc ggcgagaacc tcctgcagat gctggagacc
      541 cgcctcgaca acgtcgtgta tcgcatgggc ttcgcggtca cccgcccggc cgcgcgccag
      601 ctggtcagcc atcgcggcat caccgtcaac ggcaagtcgg tcaacctgcc gtcctacgcg
      661 gtcaaggccg gcgacgcgat cgccctgtcc gagaaggctg cccgccagct ccgggtcaag
      721 gaatcgctga ccgtggccca gcagatggac ctgtcgccgt cgtgggtcga tgtcgacgcc
      781 accaagttcg ccggcgtgtt caaggccgtg cctgcccgtg gcgacctgcc cagcgacatc
      841 aacgaagcgc tgatcgtcga gctgtactcg aagtaagcca ttgccaccgg cctgcgcccc
      901 ccgcgcaggc cagcaggaga catcacgaat gacggttacc gcaacccaag tgctgcgccc
      961 ccgcggtccg cagatcgagc gcatcgccga caaccgcgcc aaggtcgtga tcgaaccgct
     1021 ggaacgcggc tacggtcata ccctcggcaa cgcgctgcgc cgcgtgctgc tgtcctcgat
     1081 ccccggcttc gcgatcaccg aggtcgagat cgacggcgtg ctgcacgagt acaccacggt
     1141 cgaaggcctg caggaggacg tgctcgaagt cctgctgaac ctgaaggacg ttgccatccg
     1201 catgcacagc ggcgacagcg acaccctgga gctcaagaag cagggcccgg gcatcgtcac
     1261 cgccggcgac atcaagacca gccacaacgt cgaggtgctg aaccccgacc acgtgatcgc
     1321 cagcctgacc aaggacgtgg cgctcaacat gcgccttacc atcagccgcg gtttcggcta
     1381 ccagccggcc gctgcgcgcc gccgcccgga tgaagaaacc cgcgcgatcg gccgcctgat
     1441 gctggatgcc tcgttctcgc cggtccgccg cgtggcgtat gcggtcgagt ccgcgcgcgt
     1501 cgaacagcgc accgacctcg acaagctggt gctcgacatc gagaccaacg gcaccatcga
     1561 cgccgaggaa gccgtgcgca ccgccgccga catcctcagc gaccagctgt cggtgttcgg
     1621 cgacttcacc caccgcgacc gcggcgcgcc gaaacaggcc gcaagcggcg tggatccggt
     1681 gctgctgcgc ccgatcgacg acctcgagct gaccgtgcgt tcggccaact gcctcaaggc
     1741 cgagagcatc tactacatcg gcgacctgat ccagaagacc gaagtcgagc tgctcaagac
     1801 cccgaacctg ggcaagaagt cgctcaccga gatcaaggaa gtgctggccc agcgcggcct
     1861 gtcgcttggc atgaagctcg agaactggcc gccggccggg atctcggcgc acggcatgct
     1921 ggggtaagcg agcgcgctcg ggcgatgccg tccggcatcg cctgcgcgag cgaaggcccg
     1981 agcgctatgc gcgagggccg gaggtgcaaa cgcggcgcag ccgcggcctc cgtcttgttg
     2041 taccgcttca tcgcaccact tcgccgacga acgaaacgtt cgcggcatgg ccggcaaggg
     2101 acgccggcac gggccaaccg cagtccaccc gcccggaaca cggggcacaa cgccaggatg
     2161 gcgacagcga actccaatag cgccaatctt cagcccaagg aatccaagtc atgcgtcacc
     2221 agaaatccgg ccgcaagttc aaccgcacca gtgcccaccg tggcgcgatg ttctccaaca
     2281 tggcggcctc gctgttcaag aacgaactga tccgcaccac cctgccgaag gcgaaggaac
     2341 tgcgccgcgt cgccgagccg ctgatcaccc tggccaaggt cgatggcgtc gccaaccgcc
     2401 gcctcgcgtt cgcgcgcctg cgcgacaagg aagcggtcgg caccctgttc accatcctcg
     2461 gcccgcgcta cgcgcagcgt ccgggtggct acctgcgcat cctgaagtgc ggcttccgcg
     2521 ccggcgacaa cgcgccgatg gcctatgtcg agctggtgga tcgtccgcaa gccaccgagt
     2581 aagcggtagc gccagcgtcg catcaagaga ccccgggact tccggggttt tttgtttgcg
     2641 cgggtctgga ccataatcga ccgattcccc cgttggccgt gctcatgaat ccgttccgca
     2701 gcctgtcctt ccgcgcgcaa ttcctgcttg gactcgcggc ctgcgtcgcg ttgctcggct
     2761 atgcgttcta cgtgcagttc cagctgggca tccagccgtg cccgttctgc atcttccagc
     2821 ggctgtgctt cgccgcactc gggatggtgt tcctgctcgg ggcgctgcac gacccgcgtg
     2881 cgccaggtgc gcgcaaggcc tggtcggtgc ttgcggtgat cgccgccggc gcaggcgcgg
     2941 gctatgcggg ccgccattcc tgggtgcagc tcaacccgcc ggaattgccc agttgcgggc
     3001 cgggcctcaa cttcatcgtc gaacagcatt cctggctggg tgcggcgcgc gaagtgctgc
     3061 tcgcaaccgg cgactgcagc aacatcgact ggaccttcct gggcctgagc atgccgatgt
     3121 gggtactggc atggttcgtc gggctgggcc tgtgggccct gcatgccggg ttccggcgcc
     3181 gcgggaagca tcggttccac aggtaacagt tgcgatggac gggctgcaac gcgatgatgc
     3241 aatgcagcat cctcgcgatc acctccatgc cacacgccca gcacgccgtt cctcccgccg
     3301 acaacgacat cgacgcctgg gcgccggata gctggcgcac gcgccgcgcg ctgcagctgc
     3361 cgcagtaccc cgacctggcg gcgctggccg cggtgcagga cgagttgcgc gctttgccgc
     3421 cgctggtgac ctcgtgggaa atccagtcgc tcaagcagca actggcggat gcacaggaag
     3481 gcagccgctt cctgctgcag ggcggcgact gcgccgaaac cttcgccgaa tgcaattcgt
     3541 cggtgatctc gaaccgcctg aaggtgctgc tgcagatgag cctggtgctg gtgcacgggc
     3601 tgaaactgcc ggtcgtgcgg gtaggccgat tcgccggcca gtacgccaag ccacgttcgg
     3661 ccgataccga aacgatcggc gacaccaccc tgccgagcta tcgcggcgac atggtcaatg
     3721 cgccggagtt caccgcggcg gcgcgcaccc cggatccgcg gcggatggtg aaggcgcacg
     3781 cgcgctcggc gatgaccatg aacttcgtgc gcgcgctgat cgacggcgga ttcgccgacc
     3841 tgcaccaccc ggagtactgg gacctgggct gggtcgggca ggccccgctg gcgcacgaat
     3901 accagcgcat ggtcggtgcg atcggcgatg cggtgcgctt catggagacc ctgaccggca
     3961 gccaggtcca caacatcaac cgggtcgatt tctacacctc gcacgaggcc ttgctgctgc
     4021 catacgaaga ggcgcagacc cggcaggtgc cgcgccagca cggctggttc aacctcggta
     4081 cccattaccc gtggatcggc atgcgcaccg ccgccatgga cggcgcgcac gtcgagtatt
     4141 tccgcggcat ccgcaacccg gtcgcgatca agatcggccc gtcggtggcg ccggaccagt
     4201 tgctgcgcct ggtcgacgtg ctcaatcccg acgacgaacc cggcaagctg gccttcatcc
     4261 accgcatggg cgccaccgcc atcgcggaca agttgccgcc gttgctggag gcgatcaggc
     4321 gcgaagggcg gcgcgtgctg tgggtgtgcg acccgatgca tggcaacacc gaggcgaccg
     4381 ccaacggcta caagacgcgc cgcttcgaca acatccgcgg cgagctggag caggcgttcg
     4441 acgtgcatgc ggccgccggc acccggctgg gtggcgtgca ccttgagctg accggcgaaa
     4501 acgtcaccga gtgcctgggt ggcgcccgcg agctgaccga gctcgacctg cagcgcgact
     4561 accgcagcac cgtggacccg cggctcaatt acgagcaggc gctggagatc gcgatgctga
     4621 tcgtgcgcaa gcgcggcgag atcgcggcgg actgagcgca gatccggcgt acccgcggga
     4681 tcggcacgcc agccttacct ggcctcgtcc aggtcgacgg tcagcaggaa cttcggcggc
     4741 gtgcgcgcct tgctggcctg ctcgtaggcg tgggccagtt cgaccaggcg cgcttcgctc
     4801 cacgcggtgc ccatgaaggt gatgcccagc ggaaggccgt ggctgtggcc catcggtacg
     4861 ctgatgctgg ggtagcgggc caccgcggcc gcgccgtagc cggcaccggt gaagtggtcg
     4921 ccgttgatcg ggtcggtcgg ccacgccggc gacatcgacg gcgcgaccag ggcgtccagg
     4981 ttgtctttct tcagcgcggc gtcgatgccc tcggggccgg ccagtcgccg cgccttgtcg
     5041 cgggcgtcga ggtattccct gtcggtcagc ggtccctttg cctcggcctg ctcgaacagc
     5101 tcctgcgcga agtagcgcat ctccgttccc gcgttctgct tgttgaaggc gatcaccccg
     5161 gccagcgact tcaccggagc tccgctgctg gtgagatagg cattgaggtc ggccttgagt
     5221 tcgtacagca gcacctcgag ctcggcgtcg ccccattgcc cggcggtggg gatctcggca
     5281 tcgaccacgg tcgcgccggc ggccttgagc gccgccactg cgcgttcgaa cgcggcgtcg
     5341 acgtccggct ggtagcccat cgccttgcgc agcacgccga tgcgcgcgcc cttgagcgca
     5401 tcgggcttca ggtgcgcggt gtagtcgatc gcggtgcgcg cgggatgttc gctggtggcg
     5461 gcatcggcgg cgtccgcagc ggcgatcgcg gtcagcaccg cggcggcgtc ggccacgctg
     5521 cgcgccatcg gcccggcggt gtcctgcgaa tgcgagatcg ggatgatgcc gctgcggctg
     5581 accagcccga ccgtcggttt gatcccgacc aggccggcca ccgcggacgg gcagatgatg
     5641 ctgccgtcgg tttcggtgcc gatgccgacc gcggcgaggt tggcggcgat cgcggtgccg
     5701 gtgcccgcgc tggagccgca gggattgcgg tcgagcgcat acgggttcct ggtctgcccg
     5761 ccgcgcgcgc tccagcccga ggtggagcgg gtggagcgga tgttcgccca ttcgctgagg
     5821 ttggtcttgc ccaggatcac cgcaccggcg gcacgcagcc gggcaaccag gaacgcatcc
     5881 gacttcggcc ggtgctcggc cagcgcaagc gagccggcgg agttcaccat cggggtggcg
     5941 tcgatgttgt ccttcagcag caccgggatc ccgtgcagcg ggccgcgcag cttgccggac
     6001 gtgcgctcgg catccagcgc atcggcttcc ttcaatgcgt cgggattggc ctcgatcacg
     6061 gcattgagcc gcgggccggc gtcatcgatc gcggcgatgc ggtccaggta ggcctgggcc
     6121 agtgcgcggc tggtcagcga gcccgaggcc atctgctcct ggagcgaggc gatgctggct
     6181 tcctcgaacg caaaaggcgc gggtgccgcg gttgccgcgg cttccgccga ggcaggctgc
     6241 ggggtgtcgg tttgttggca ggcggccagc agcagcagcg cgatcgcgag cgagaggcgg
     6301 gtcatcggcg aggctccggg cgggaatgcg caagcatcgc ccgcgcaggc cgcgcgggca
     6361 acccggcggt gtgcctggac ccgcggctgg cagttacgcc gagcggctgg cccgcttgcg
     6421 gtcgctttcg gtcaggaact tcttgcgcag gcggatttcc ttcggggtga cttccaccag
     6481 ctcgtcgtcc tcgatgaagt ccagcgcctg ctccagcgtg tacttgatcg ccgggctcag
     6541 ctggatcgcg tcgtccttgc cggaggcgcg catgttggtc agcggcttcg gcttgatcgc
     6601 gttgaccgtc aggtcgttgt ccttggagtg gatgccgacc agctggcctt cgtacacgtt
     6661 gtcgccttcg gcggcgaaca gcttgccgcg ttcctgcagc ggcccgagcg aataggccgg
     6721 ggtggtgccg gcggcattgg cgatcatcac gccgttctgg cgcttggcga tcgcgccggt
     6781 ttccttcggg ccgtaatggt cgaacacgtg gaacagcagg cccgagccct gggtcagggt
     6841 gcggaactcg ttctggaagc cgatcaggcc gcgcgccggg atcatgtagt caaggcgcac
     6901 gcggcccttg ccgtccgatt ccatgttctt cagcgcggcc ttgcgggtgc ccagcttctc
     6961 catcacgccg ccctggtgga cttcctcgat gtcgaccacc agttgctcga ccggctccat
     7021 ctgctggccg tcgatttcct tgatgatcac ttccgggcgc gacaccgcca gctcgaagcc
     7081 ttcgcggcgc atgttctcga tcagcaccga caggtgcagc tcgccgcggc cggagaccag
     7141 gaacttgtcc ggatccgagc cttcctcgac cttcagcgcc acgttgtgca gggtctcgcg
     7201 ctccagccgc tcgcgcagct ggcggctggt caggaacttg ccgccgctca tgtccttgtt
     7261 gccggcgaac ggcgagttgt tgacctggaa ggtcatgctg atggtcggct cgtcgacggt
     7321 cagcgccggc agcgcttccg gcgcgtcgag cgcgcacacg gtgtcggaga tcgacaggtc
     7381 ggcgacgccg gaaatggcga cgatgtcgcc ggcgccggct tcctcggttt cgatgcgctc
     7441 caggcccatg aagcccagca cctgcagcac cttgccctgg cgcttcttgc cttcgcggtc
     7501 gaccacgctg accggcatgt tgcggcggac cttgccgcgc tggatgcggc cgatgccgat
     7561 caggccgacg aagttgctgt agtccagctg gctgatgcgc atctggaacg ggccgtccag
     7621 gtcgacctgc ggcggtgcca cgtgctgcat gatcgcttcg tacagcgggg tcatgtcgcc
     7681 ttcgcgcgcg ctgtcgtcga ggcttgcgta accgtgcagc gccgaggcgt agacgatcgg
     7741 gaagtcgagc tgctcgttgg tcgcgccgag cttgtcgaac aggtcgaaca cctggtcgat
     7801 cacccagtcc gggcgcgcgc ccgggcggtc gatcttgttg accaccacga tcggcttgaa
     7861 gcccatcgcg aacgccttct gggtcacgaa gcgggtctgc ggcatcgggc cgtccatcgc
     7921 gtcgaccagg atcagcaccg agtccaccat cgacagcacg cgctcgacct cgccgccgaa
     7981 gtcggcgtgg cccggggtgt cgacgatgtt gatgcggttg ccctgccagg tgatggcggt
     8041 gttcttggcc aggatggtga tgccgcgttc cttttcctgg tcgttgctgt ccatcacgcg
     8101 ctcggccagc accgtgcgtt cgttgagcgt gccggactgc ttgagcaggc agtcgaccag
     8161 ggtggtcttg ccatggtcga cgtgggcgac gatggcgatg ttgcgcaggc gttcgacgga
     8221 ggaattggat tcggacataa gcaatgggcg caccgcggtt caggcaggac gcagcaggtc
     8281 tgggaaggaa gccggctatt atagccggcc ccggggtcgg ccgtctgacc cggttcctgc
     8341 aaaatacttc ccgaaggaat ccacatgtcc ctgatcgcca ccttcgacac cgaccgcggc
     8401 ccgatccgcg tcgaactggc cgccgaaaag gcaccgctga ccgtcgccaa cttcgtcaac
     8461 ctggccaggc gcggctttta cgacggcctc aacttccacc gggtgatccc cgatttcatg
     8521 gtccagggcg gctgcccgga gggttccggc cgcggcggcc cgggctaccg gttcgaggac
     8581 gagaccaaca acggcgtgaa gcatgagcgt ggggtgctgt cgatggccaa cgccggcccc
     8641 aacaccaatg gcagccagtt cttcatcacc cacatcaaga ccgattggct ggacggcaag
     8701 cacaccgtgt tcggcaaggt catcgaaggc ctggacgtgg tggatgcggt gaagcagggc
     8761 gacctgatca agtccgtgaa gatcgaaggc gatgccgagg cggcgctggc cgcccgggcc
     8821 gaccgcgttg cggagtggaa caagatcctc gccgcctgag ccatcggcgc ggcgcatgga
     8881 gagggcggct ttggccgccc ttttcgttgg ctcgggtgcg gcggcgatgg cagcggcgcg
     8941 gcctgccggc agcggccgac cccgacgcgc tgcgtgctaa aatttcgcgg ctttacccca
     9001 cgtccatgcc gcggatccgc ggcccgcacc gaggttcccc cacgatgcct caactcgccc
     9061 ggcgcatcgg ccgcgccaag cccagcgcga tcatgcaggt cgccgagaag gccaagcgcc
     9121 tcaaggccga aggccgcgac atcatcagct tctcgatcgg cgtgccgaac ttcctgccgg
     9181 cggagcacgt gtatgccgcc gcgcgcgacg cgctcgccag ggacagcggc cagtacggca
     9241 gcaaccgtgg cagcgacgcg ctgctggacg ccttcatcgc gcacatggcg aagatcggcc
     9301 tggccggcta cacacgcgcc aactgcgcga ccgggatcgg cgccaagcac gtgatctaca
     9361 acctggccga ggcgctgctg gacgaaggcg acaccatcgt gttcccggtg ccgtactgga
     9421 ccagctacat ggacatcgcc gatatcgtcg gcgcccgggt cgagctgctg ccgtgcccgg
     9481 cctcgcagga ctacaagatg acgccggccc agctcgatgc cgcgctggcc tcgaagcccc
     9541 gggtgttcct gttcaacaac ccgtccaacc cgaccggcat ggtctacaac cgggatgaga
     9601 tcgtggcgct ggccgaggtg ctggtgaagc atccggacac gtggatcatc accgacgaca
     9661 tctacaaccg gatgctgttc gatggcctgg agtacgcgaa cttcgtgcag gtccgcccgg
     9721 aactgcgcga ccgcgtgatc ttcatcgact cgctgtccaa gacctacggc atgcccggtt
     9781 ggcgggtggg cttcatggcc ggcccggagg cggtggcgca ggccctgacc accatgaatt
     9841 ccaaccacat caccaacatc cccgagatcg tcaccgcggc cgcgatcgcc gcgctcaacg
     9901 gcccgcagga cgtgccgacc cagaagaacg ccgagttcca ggccaagcgc gaccaggtca
     9961 tggcggtgat ggatgccatc cccggcgtgg tctgcccgcg tccgcagggc gcgttctacg
    10021 tgttcccgga catcagctgt gcgttcggca agtcgcacaa cggcacgaag atcgggaacg
    10081 acgtggactt ctgcaatgcg ctgctggaag ccaagggcgt ggcctgcgtg ccgggctcgg
    10141 cgttcggcga gccgcgcgcg ctgcggatca gctacacctg cccgaccccg cagctggcgc
    10201 ccggcctggc gcgcatccag gaattcttcg ccgagttgac ctgatccttt ccgttcccgc
    10261 cggtgcggcg cttgcgccgc atcgtcattt ccaggagttg aacccaatga aagcccccgt
    10321 tcgagttgcc gtcaccggtg ccgccggcca gatcggttac gccctgctgt tccgcatcgc
    10381 ctccggcgaa atgctgggca aggaccagcc cgtcatcctg cagatgctgg aactgccgat
    10441 ggagaaggcc caggccgcgc tgcgcggcgt gatgatggag ctggaagact gcgcgttccc
    10501 gctgctggcc ggcatggtcg gcaccgatga cgccgaagtc gcgttcaagg atgccgacta
    10561 cgccctgctg gtcggcgcgc gtccgcgcgg cccgggcatg gagcgcaagg acctgctgct
    10621 ggagaacgcc aagatcttca ccgcgcaggg cgccgcgctg aacaaggtcg ccagccgcaa
    10681 cgtcaaggtg ctggtggtcg gcaacccggc caacaccaat gcctatatcg cgatgaagtc
    10741 ggcgccggac ctgaagccgg gcaatttcac cgcgatgctg cgcctggacc acaaccgcgc
    10801 gctgtcgcag ctggccatca aggccggcgt ggccgtggcc gacatcgaga agctggtcgt
    10861 gtggggcaac cacagcccga ccatgtaccc ggattaccgc ttcgccacgg tcaacggcga
    10921 gtcgttgaag gacaaggtca acgacgccga gtggaacgcc aacacgttca ttccgcaggt
    10981 cggcaagcgt ggcgccgcga tcatcgaggc acgcggcctg tcgtccgccg catcggccgc
    11041 caacgccgcc atcgaccaca tgcgcgactg ggcgctgggc accaacggca agtgggccac
    11101 catgggcgtg ccgtccgacg gcagctacgg cattccggaa ggcgtgatct acggcgtgcc
    11161 cgtcacctgc gccaacggcg agtacacccg catcgaaggc ctgccggtcg atgacttcag
    11221 ccgcgcggcg atggacaaga ccctggccga actggaagaa gagcgcgccg gcgtggcgca
    11281 cctgctgtaa gccaccgcca ccgctgcgag ccggaagggc tggccttgtg ccggcccttc
    11341 ttgcgttggc tggcatgagt gcgcagctgc ggtcactgcg gacccgggcg ccggcggatg
    11401 ccggggccaa ggcaatccgg ccttggacgc cagtgcccgg cggcctgcat cgggcagccg
    11461 cggcccatgc atgaggggga ggcgatgggg cagcagtggc gaatcgcagg gtcgcgcaat
    11521 gcgtgcggcc tgccgcgcgg ggcgggccgc ctccggcaac gtgatccgcc atgatcctgc
    11581 gccgcgtcat ccagcacgtc cgcaagcagg aatggaccgc catcgccatc gatttcgcca
    11641 tcgtggtgat cggcgtgttc gtcggcatcc aggtcgcgaa ctggaaccag gagctcgccg
    11701 atgaacgcct cggccacgcc tatgccctgc gcctgcaggc ggatctcaag cgtgacctgg
    11761 tcgcgcggcg cgagctggtg gattaccacg cggcggtcct gcggggcgtg gagcggaccg
    11821 acgcgctgct tgcggacccg cgttccgacg cgaaggcgct ggtggtcaat gcctaccgcg
    11881 ccagcgaact caactaccgg gcgacttcgc gcgcgacctg ggatgaaatc gtgtcgtccg
    11941 cggacaccgg cctgctgcct cccggcgtgg cgcgcagcgc cggcgagtat ttcgcgatcg
    12001 actccgcgcg cctgaccctc gacggcctca cccagtccgg ctaccggcat cgcgtgcgca
    12061 tgatcatccc gcatcgctcc gaccgcccga cccatctgcc gatgcaggcc aggcgatgaa
    12121 attcatcagc gaactcaagc gccgcaacgt catccgcatg gccgggctgt acctggtcgg
    12181 cgcatggctg gtgacgcaag tcgcggcgac cctgttgccg gtgttcgagg cgccggcctg
    12241 ggtgatgaag gcggtggtgg cggtactggc ggtcggcttc gtgcccgccc tgatcgtggc
    12301 gtgggcattc gagttgactc cggaaggcct gaagcgcgac ggcatggtgc cgccctccga
    12361 atccatcgcc ccgcagaccg cgcggcggat ggaccgcatg ctgttggtgg tctcggcgct
    12421 ggcgctcgcc tatttcggct tcgacaagtt cgtgctggcg ccgcaacggg acgcggccct
    12481 ggtggcgcaa acgacccgtg cggtggtcgc caacgcaggc gccagggcag cgaagcaagg
    12541 gccggatccg cgttcgatcg cggtgcttcc gttcgccaac atgtccgccg acccggaaaa
    12601 cgagttcttc tccgatggcc tgtcagagga agtcctgaac tcgctggcgc gcatcgaccg
    12661 catgcaggtg gtcgggcgca cctcgtcgtt ccagttcaag ggcaagaacg tggacctgcg
    12721 cggcatcggc cagaagctcg gcgtcgccca catcctcgaa ggcagcgtgc gccgcgatgg
    12781 cgagcgcgcc cgcatcaccg cccagctgat ccgcgccagc gacggtgtcc acctgtggtc
    12841 gcaaacctac gatcgcacgc tgcaggacac gctggcggtg caactggaca tcgccgaaca
    12901 ggtggccggt gcactcggcg tgctgctgga cgacaagcaa cgcggcacca tgcgcaaggc
    12961 cggcgtgggc aatgtggatg ccttcatcca ctaccagaag gcctggaagt tgtaccttgc
    13021 cgcgcacggc gacaagtcgg tcggcctgct cggaggcctg cagctggcga acgcggaatt
    13081 cgaccgcgcg atcgcgctgg agccgaatta ctcgatggcg cattacgcca aggccgatct
    13141 ctacgaacac acactgatcg acgaccacac cacccacgcc gagcgcgcgg atgcgcagcg
    13201 cgcagtcctg cagacgctgc agcgcgccgc cgccaccggc ccggatgcgc agcagcgcga
    13261 attcgccctg gccgagcggc agatgctcgg cgacgactgg cgcggcatca ccgatcaatt
    13321 gtcgaaagcg cttgcgcaac ccggctgcgg cgcgccgaac tggatgccgg tcttcgccag
    13381 cgccttcggc tatgccgatg cgctggaacc cgtgctggca cgtgcggcga cctgcgaccc
    13441 attgaatgcg atcaactgga acacgcgcac ctgggtcgcg cgcgcggatg gcaaggccga
    13501 acgcatccag gcgatctacg cgcagtggcg gacctacgcg ccggcattgc cgcaagcacc
    13561 gcatgaacgc ctcgcgttgg ccatgctcgg acacgaggac aagttgccac cgaagacgaa
    13621 gaagctggac aacctcggtg cggcagagtt cggtaccgcg atgcagatcg ccaggttgcg
    13681 cgggctgaat cgtgcgcagg cgcaggcatg gaccgccggc cgacgcaacg aagacgccaa
    13741 cataggtcgc cagtggcaga tcgtggaact ggtcgaagcc gcgctgttcg gcgaccgcgc
    13801 ggatgccaac cgccgcgccg ccgtgctcga cgcgcgcccg gccggcggct tgctgctcgc
    13861 cgtcgcggtg acctattgcg gctgcggcgc gccgttcgac ctgtccgcca caccgaattt
    13921 caaggcgcgc ctggccgaat ccgggttgcg atggccacct cccgtggcga tccgctatcc
    13981 gccgatcccg gggacggctc cttgagcttc ctcgccgaac tccgccgccg catggagcgc
    14041 atgttcgtgc tgctgctggc gctggcgttg ggcctcttcg ccttcgacag gttcgcgctg
    14101 gcgccacgcc gggaggctgc cctggtggcc gcgaccacgg agacggcgaa ggccgaagcg
    14161 ggcgcaggca agcaggcggc gatcagcgag aaagccatcg cagtactgcc gttcgtgaac
    14221 atgagcgcgg acagggacaa cgagtatttc tcggacggca tcgccgagga gatcctcaac
    14281 gcgctggcgc aggtgaagga cctcaaggtg gcagggcgca cctcctcgtt ccagttcaag
    14341 ggccgcaacg agagcctcac gtccatcggc caggccctgg gcgtggcgaa cgtgcttgaa
    14401 ggctcggtgc gcaagcaggg cgacaaggtc cgcatcaccg cgcagcggct accgccgcgc
    14461 cgcggtggag cgcatcgaac gtgcgctggc gatcgatccg atggtgccca acgtgatgcg
    14521 ttggcgcggc gtgctctacc tgcgcgatgg caacatcgac ggcgccgagc aatacctcaa
    14581 gcgggccaag gcgacaggcc tgcggctggc ggatcgcgaa cttggcgaaa tcgcccactt
    14641 gcgtggcgac acggcttcgg cgcgacgcat ctggaacgac tcctcggacc cgtcgctcgg
    14701 gcaaatggcg cgggacgcgc gaaaggcgct ggccatcgcc atgtccggcg gtgacgccgt
    14761 ggaacgcgcc cgcgcagtga gcgcggtcga ggcctacgtg gcctccgggc cggaattcct
    14821 gcccggcatc gtgccgatgt ggctggcgca gatcggccag gaggcgcgcg ccctggaact
    14881 cgaccgcacc cgttcggagg tcgacaacag cgacttcatg gtctacctgt tcagcccgga
    14941 cgggaagtcg ctgcgcgccc tgcccgagtt ccccgcctac atgcgcgcga agggtttccc
    15001 ggcgctgtgg gacaagtacg gggcgccgga catgtgccgc aaggatgccg ccggcgatta
    15061 ccgctgcgac tagcgccgtt cagccggcgc caccgcggcg cgggagcttc cagcccggcc
    15121 gcacgaagtg gcaggtgtaa ccgccggggt agcgctgcag gtaatcctgg tgctcgggct
    15181 cggcttccca gaacggcccg gccggcgcga gttcggtcac caccttgccg ggccacaggc
    15241 cggaggcttc aacgtcggcg atggtgtcca cggcgatccg gtgctgttcg tccgaggcgt
    15301 aatagatggc ggagcgatag ctgctgccgc gatcgttgcc ctgccggttc acggtggtgg
    15361 gatcgtggat ctggaagaag aactcgagga tgtcgcgata ggacgtgcgc gccggatcga
    15421 acacgatctc gatcgcctcc gcgtgcgtgc catggttgcg ataggtggcg ttggcgacat
    15481 cgccgccgga atagcccacg cgggtggcca gcacgccggg cagcttgcgc accagttcct
    15541 gcatgcccca gaagcagccg ccggcgagga tcgcgctttc ggtggaggtg ctcatgcgcg
    15601 gtctccggcg aacaggtggc ggtagtcgcc gtagccttcc tgttccagca catctgccgg
    15661 gatgaagcgc agcgcggccg agttgatgca gtagcgcagt ccgccggcgt cgcgcggacc
    15721 atcggtgaat acgtggccga ggtggctgtc gccgtgtgcc gaacggactt cggtgcggcg
    15781 catgccatgg ctgctgtcgc tgcgctccag cacatgcgcc ggttccaccg ggcgggtgaa
    15841 actgggccag ccgcagccgc tgtcgaactt gtcgagcgag gtgaacaggg gttccccgga
    15901 cacgatgtcg acgtacaggc caggtgcctt gctgtcgtgg aaggcgttgt cgaacggtcg
    15961 ctcggtgccg gcgtcctggg tgacccggcg ttgctccggg gtcagccggg cgagcgcttc
    16021 cggggtcttg ctgtaggtct tggccatggc ctggttccct gagcggtggg cttgattccg
    16081 tggtgatgat gtaggggcgg gtgagggcgc cggcaagtgc cggccggcat gcgcggcggc
    16141 cgggcagggc gatcggctaa aatcacggtt ttaccggagg ctccatggac gaacgccagt
    16201 ccgcaggcgc gcagttccgc gccgcgcttg ccaccgaatc cccgctgcag gtgatgggcg
    16261 cgatcaccgc ctacgccggc ctgatggcca ggcgcactgg ctacagggcg ctgtacctgt
    16321 ccggcggcgg cgtcgccgcg aactcgctgg gcgtgcccga cctcggcatc tccacgatgg
    16381 aagacgtgct gaccgacgcg cgccggatcg tggacgccac cggcatgccg ttgctggtcg
    16441 acatcgacac cggctggggc ggggcgttca acatcggccg caccatccgc gccttcgagc
    16501 gcatcggcgt ggccgccgtg cacatggagg accaggtcgg gcagaagcgc tgcggccatc
    16561 gtcccggcaa ggaagtggtg acgaaggagg aaatgatcga ccgcatcagg gccgcggtcg
    16621 acgcgcgcac ggatcgcgat ttcgtgatca tggcgcgcac cgacgccgcc gcggtggaag
    16681 gcctggacag cgcgatcgag cgcgccgtcg cgtatgtcga agccggcgcc gacatggtgt
    16741 tcccggaggc gatgacctcg ctcgacgact accgcaaggt caaggcggcg gtgcaggtgc
    16801 cgatcctggc caacctcacc gagttcggca gcacgccgtt ctacaccacc gacgagctgc
    16861 gcgatgccgg cgtcgatatc gcgctgtatt gttgcggtgc ctatcgcgcg atgaacaagg
    16921 ccgcgctcaa cttctacgag gccgtgcgcc gcgacggcac ccagaagcat gtcatcgaca
    16981 ccctgcagac gcgcgccgaa ctgtacgact tcctgggcta ccacgcctac gaagacaagc
    17041 tcgatgcgct gtttgcgcag aacaggtaat cgaaggaata aacgatgagc gaacagtccg
    17101 cagccacgcc cggcttcaag ccgaagaaat ccgtcgccct cagcggcacc gctgccggaa
    17161 ataccgcgct gtgcacggtc ggccgcagcg gcaacgacct gcattaccgc ggttacgaca
    17221 tcctggattt cgccaatgcc agcgagttcg aggagatcgc ctacctgctg gtgcacggca
    17281 agttgccgaa caaggcggag ttggcgggct acaaggccag gctgaagtcg ctgcgcggca
    17341 tcccggcggc ggtcaaggcc gcgctggagg aactgccgcc gtcggcgcac ccgatggacg
    17401 tgatgcgcac cggcgtgtcg gtactgggtt gcgtgtcgcc ggagaaggac gaccacaacc
    17461 atcccggcgc gcgcgacatc gccgacaagc tgatggcgtc gctgggttcg atgctgctgt
    17521 actggtacca ctggagccac aacggccgcg ccatcgacgt cgagaccgac gacgacagca
    17581 tcggcggcca cttcctgcac ctgctgcatg gcgaagcgcc gcgcgagtca tgggtgcgtg
    17641 cgatgcacac cagcctgatc ctgtacgccg agcatgagtt caatgcgagt acgttcaccg
    17701 cacgcgtcat cgccggcacc ggcagcgaca tgcattcgtg catcgcgggt gcgatcggcg
    17761 ccctgcgcgg accgaagcac ggcggcgcga atgaggtcgc gttcgaagtg caaaagcgct
    17821 acgagaaccc ggacgaagcg gaagccgaca tcaaggcgcg ggtcgagcgc aaggaagtgg
    17881 tgatcggctt cggccacccg gtgtacacgg tgtccgatcc gcgcaacaag gtgatcaagg
    17941 acgtcgcgcg cgagctgtcg gcggagcagg gcgacatgaa gatgtacgac atcgccgagc
    18001 ggctggagtc ggtgatgtgg gacatcaaga agatgttccc caacctcgac tggttcagcg
    18061 cggtcagcta ccacatgatg ggcgtgccca ccgcgatgtt caccccgctg ttcgtgatcg
    18121 cgcgcacgtc gggctggagc gcgcacgtga tcgagcagcg catcgacggc aagatcatcc
    18181 gcccgtcggc caactacacc gggccggaag accgcgtctt cgtcccgctc gcgcaacgct
    18241 gaagccggac gccgcggtgg actggctgat ccccgccggc tatttcgccg agtacctgct
    18301 ggtggcggtg gtcgccttcg cggtcaccgc gctggtcgtc gccacccact acgagggcgt
    18361 gcatgcgctg gcacggcgtt tcgcgtcgcg cgcgccgcgg cgcgaccacc tcgcgatgct
    18421 caagatcatc ttcgcgctgc tggccctgca catcgtcgaa atctggtact acgggctggc
    18481 ctactggggg ctggtgatga tcccgggtac cggtttcgtg catggcgaat tcggcatgga
    18541 ccgcctgttc gacgcggtct acctgtcggc catcacctac agcacggtcg gcttcggcga
    18601 cctggcaccg gtgggggcga tccgcctggt cgccgggctg gaagccctga ccgggctgct
    18661 gatgatcacc tggtcggcgt cgttcactta cctggagatg tcccgcctgt gggcggacga
    18721 gcgccggcgc tgagcgtgcg cgggctcagc cgcggctgcg gcgtggcctg agcgattgca
    18781 cctgcaggaa ggtgtggtcg ccgatggtcg ccacctggta ggcgttgcgc cagctcgggc
    18841 tggcgatcgc gtgcgccatg aagtggctgg caccgggcac gatctcgcgg cgctggcctg
    18901 ccggccgcgc ccagttgcgc tcggactcga gcgcgatggt gaccgcctcc gcccaggcct
    18961 cgcggttgga caggcgggtc tgcggcgaca cgatggtcgg cgcgaactgc ttgcgcgcgg
    19021 tcaccacctg gcacaggttg cggccccaca gcccgctgtc gcggcggcgc agggcgactt
    19081 cggcgaccgc ctgctggccg cgcacggact ggtcgcgtgc ctccaggtag acggtggtcg
    19141 acaggcaaag cgaatcggcg gccggctgcg gcagcacgtt gccggccatc cagaggatcc
    19201 aagccagttt catgtcgtac tccttgctcc gttgcggcgc aggccccggc gccggcagga
    19261 ccggcgagga agcaagcgtc atggcgtact ggggagggcg gcagctctgt ggcggccttt
    19321 gcccgggcgc gccggccctg ggagaggggt cggtcggcgt gccggtggcc tgtcgatgcc
    19381 gcgcagcagg cggtccggca gggtcaaaag tccgcgcacc atatgcgccg gatacctaac
    19441 ggttcctgaa cgaataaacc agaagtcgat gattcgattg aagtttttac tctgcaggct
    19501 tgctcagagc gatgccgcca cgtggctgcc ctggctgatt gcacgcttcg cgtccagttc
    19561 gctggcgaca tcggcgccac cgatcaggcg tggctggcgc ccctgcgcca gcagtgcgtc
    19621 gaagagttcg cggcgcgatt cctggccggc gcagaccacg acgtggtcga cctccaggac
    19681 ctgttcgcca ccgtcgacgc ggatgtgcag gccggcatcg tcgacgccca ggtattccac
    19741 cccgcccagc atcttcacgc ccttcgcctt cagcgtgctg cggtggatcc agccggtggt
    19801 cttgcccagc cgggcgccgg gcttgccctg gctgcgctga agcagccaga ccttgcgcgc
    19861 ggcgggttcc ggttgcggcc ggaccaggcc gccgggcgac tcgaacgctg cgtcaacgcc
    19921 ccattcggcc atccagcgac cgctgtcgag ggtgggcgac ccgccttcct ggaccaggaa
    19981 ttcggcgacg tcgaagccga tcccgccggc gccgatgatc gcgaccgtct tgcccggctc
    20041 cacgcgtccc tgcaggacgt cgaggtagcc gacgaccttc ggatgatccg cgccggggaa
    20101 atcgaccgcg cgcgcagtca ccccggtcgc caccacgact tcatcgaagc cggacaagcc
    20161 ggccgcatcc acccgcgtgt gcagccgcag ctcgaccccg gtctccgtga tgcggtggcc
    20221 gaagtagcgc agggtctcgt ggaattcctc cttgcccggg atcgtcttgg cgaggttgaa
    20281 ctggccgccg atttcgccgg cggcatcgaa cagggtcacc cggtgcccgc gctcggcggc
    20341 gatggtggcg caggccaggc cggcgggacc ggccccgacc acggcgaccg atttcggcgt
    20401 ggtcgtgcgc gtgtagttca gttcggtctc cgcgcaggcg cgcggattga ccaggcagct
    20461 ggccggcttg ttctcgaaca cgtggtcgag gcaggcctgg ttgcaggcga tgcaggtatt
    20521 gattgcgggg ctacggccgg cgcgcgcctt gttcacccat tgcggatccg ccagcagcgg
    20581 gcgcgccatc gacaccatgt cggcgaagcc ggacgcaagc accgattcgg ccacgtccgg
    20641 catgttgatg cggttggtcg cgaccagtgg cagccgcacg tgcggcttga gcttcgcggt
    20701 caccccgacg aacgccgcgc gcggcaccga ggtggcgatg gtcggcaccc gcgcctcgtg
    20761 ccagccgatg ccggtgttga tgatggtcgc gccggcggct tcgaccgcct gcgcctgcgc
    20821 gacgatggcg tcccagtcat tgccgccttc gaccaggtcg agcatcgaca accgatagac
    20881 gatgatgaag tccggtccgc aggcctcgcg cacgcggcgc acgatctcca ccgcgaagcg
    20941 catgcgcttg ccagcgtcgc caccccacgc gtcgctgcgc ttgttggtgc ggccgaccgt
    21001 gaactggttc agcaggtagc cttcggaacc catgatctcg acgccgtcgt agccggcttc
    21061 gcgcgcgagc ttggcggcgt tggcgaaatc gcggatctgg cgctcgaccc cgcgcgccga
    21121 caacccgcgg ggagtgaacg gattgatcgg cgccttcagc ttcgacgccg acaccgacag
    21181 cgggtggtag gcatagcgcc cggcgtgcag cagttgcagg cagatcttcg cgtcgtgcgc
    21241 atgcaccgcc gcggtgacct gccggtgccg gttcacgtgc cacggccagc tcatgcgccc
    21301 ggcgaacggc ttcagccagc cgaccaggtt cggcgcgaag ccaccggtca cgatcaggcc
    21361 caccccgccg gccgcgcgct cggcgaagta ggccgccaac ctggggaaat cggaggcgcg
    21421 atcctccagg ccggtatgca tcgaacccat cagcacccgg ttgcgcaggg tggtgaagcc
    21481 gagatcgagc ggccggaaca ggtgcgggta gggcgaggac tcgttcatcg cgcaagcata
    21541 ccggcgcgta tgggtgtctg catccacgcg ggcgggtgct gcgtccggat gcagggactg
    21601 gtttgcaacc cgggccagca ccagcggctg gctttcgcta tgatgcaggt caggcaggtg
    21661 aacggctttg catgcctgca actgttatgg tagcgttaac acggctttca cgcggccgac
    21721 gccaccctgt gcagcatggc gtgcggacat agccgtccat acattgaata tttcgcgtca
    21781 caggtttctt tactgatcaa ggagctgaac atgaataaga aactcctctg cgctgccctt
    21841 ctgggtggtc tgggtctggc gcaggctgcg tccgcccagg agtttgatga tcgctggtat
    21901 gt
//
LOCUS       DAORUK010000019        21366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_2041_length_21366_cov_18.476890, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000019 DAORUK010000000
VERSION     DAORUK010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 21366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21366
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2041_length_21366_cov_18.476890"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1941
                     /gene="metG"
                     /locus_tag="PK818_02415"
     CDS             <1..1941
                     /gene="metG"
                     /locus_tag="PK818_02415"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HQY81622.1"
                     /translation="HFVCADDTHGTPIMLAAEKAGTTPESFIAAVQAGHERDFAAFGV
                     AFDHYDSTNSAANRALTEQIYARLDAGGHIARRSVAQFFDPAKGMFLPDRYIKGTCPN
                     CKAADQYGDNCEVCGATYGPTDLIEPKSVVSGATPEMRDSEHYFFEVGRFEAFLREWL
                     ASDTLLPGVKAKLMEWLDADGGLRAWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYL
                     SSFRNLCAKTGIDFDAFLKPGSDAELHHFIGKDIVNFHGLFWPAVLHGAGMRTPTRLH
                     VNGYLTVDGAKMSKSRGTFVMARTYLDAGLDPEALRYYFACKTSGGVDDLDLNLADFV
                     ARVNSDVVGKFVNIASRCAGFIDKRFDGRLAAALPDPAQYQRFVEALGGIADAYARNE
                     AATALRLAMTLADEANKYIDEHKPWVLAKQDGADAQLHAVCTQALNLFRVLAVALKPV
                     LPRTTAQADAFLDAPVAGWDDITAPLLARRIQPYAPLFTRIDPKHIQTMIDASKDTLA
                     APAPTAAAAPAKAAPADATPAPKAGTDTAQIGIDDFAKLDLRIGKVLECGFVDGSDKL
                     LRFLLDAGDLGQRQIFSGIRASYAEPEKLVGRSVVFIANLAPRKMRFGTSEGMILSAG
                     FDGGSLSLLDADEGALPGMPVR"
     gene            2072..2491
                     /gene="rnfB"
                     /locus_tag="PK818_02420"
     CDS             2072..2491
                     /gene="rnfB"
                     /locus_tag="PK818_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf electron transport complex subunit RnfB"
                     /protein_id="HQY81623.1"
                     /translation="MTALPTTDLIERLDRILPQTQCGQCGYDGCRPYAEAMARGEAGI
                     DHCPPGGDAGARALAKLLGVDARPYDRSRGIRKPAQLALIVEADCIGCTKCIQACPVD
                     AIVGAPKFMHTVVDALCTGCELCVPACPVDCIVMLPP"
     gene            complement(2589..3545)
                     /locus_tag="PK818_02425"
     CDS             complement(2589..3545)
                     /locus_tag="PK818_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HQY81624.1"
                     /translation="MQSRLLCLALAAALAVPAAFAEQPQAVAAPTAQAARHALLWKVS
                     DADNSVYLLGSFHLLKPDDYPLPKEIDAAFEDAESLLFEVDPREMSAPETLATIQKYA
                     AYDSGKSLGTVLPKATHEKLATLLATSGGSVQAFEQTEPWMVSMSLVLGLTHAMGFRA
                     ELGMDRHLMARAAEAGKPAAGLETIEDQMRAMDSVSHAEQAQGLAEFLDDPRHAMQQM
                     QDMHGWWRSGDAAKLDSEMRAEMARKSPQSYRLLDIDRNNAWLPKVEARLAEATVDDT
                     LVVVGSLHLLGNDGLVSKLRAKGYVVERVCSGCEGTSLQQVH"
     gene            complement(3551..4510)
                     /locus_tag="PK818_02430"
     CDS             complement(3551..4510)
                     /locus_tag="PK818_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HQY81625.1"
                     /translation="MVPARTLTLLAAVIAAGVAACQPAQAPVASRQARAEEAAFQARQ
                     QAWRAGRVADLTRPDGWTSLTGLHWLDPGAHRVGSDTDNGIRLAVGPEHLGVFTVRGD
                     KVGFVPDTVVMVDGEPGLGASTLRIDTDPAGPSKLVFDGGKGLATVIERGGRLALRVK
                     HADAESRLQFTGLEYWPGGRDWQVAARFVPHAAGKTLPIANIIGTTDEVPNPGAVEFE
                     RGGKTYRLEALDQGEGTLFLVFADRTNGRGSYGAGRFIDAPKPDAQGRVLIDFNQSYN
                     PPCAFTAFATCPLPPPENRLDLAVEAGERDYRKRAAPKPPTEG"
     gene            complement(4489..6294)
                     /gene="mutL"
                     /locus_tag="PK818_02435"
     CDS             complement(4489..6294)
                     /gene="mutL"
                     /locus_tag="PK818_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HQY81626.1"
                     /translation="MSIRQLPDTLINQIAAGEVVERPASVVKELVENALDAGAQRIDI
                     DLEEGGVRLIRIRDDGGGIAPEELPLAVSRHATSKIASIDDLEAVATLGFRGEALPSV
                     ASVSRFRLASRRHDAQHGAELRIDGGKPGDVVPQAHPPGTTVEVRDLFYNVPARRKFL
                     RAERTELSHIEEWLRTLALARPDVEIRVSHNGKPSRRWKGEGHLLSDVRLHEALGEDF
                     ARNALRVDHAGAGLRLHGWIAQPAYNRASADQQYLYVNGRSVRDRNIAHAVRQAYSDV
                     LFHGRHPAYVLFLELDPRGVDVNVHPAKHEVRFRESRLVHDFVYRTLHAALAETRAGS
                     AGVPMQQHAPSPSWSMPPPQQASMGLRVGDAPASYAALYGTDHAAIHGEPWPASDSPA
                     QPSLAGRAQTLDAPAHPPLGFAIAQLHGIYILSENADGLIVVDMHAAHERIGYERLKA
                     AHDGAGLRTQPLLVPSAIAVSEREADVAEREADTLAQLGFEVQRSGPQSLLLRCVPAL
                     LAHGDVEALLRDVLTDLREHGSTRRVAETRDALLATMACHGAVRANRRLTLPEMNALL
                     REMEATERSGQCNHGRPTWTRFNLAEIDRWFLRGR"
     gene            complement(6318..7724)
                     /locus_tag="PK818_02440"
     CDS             complement(6318..7724)
                     /locus_tag="PK818_02440"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQY81627.1"
                     /translation="MLLAALCWNLANASEIKHLRVDTGATGTRAEVLLDREGAYKLIE
                     LRNPDRLVVDFPGSHLGRGLSLPGAAGVVKAVRSGQPEPGTARIVFDLSSGVAVLTPR
                     IEQGTEGPRLVLEWPGDGVADAVPPAKPEATAVAAASDPIAAIASASANVPVAPAATG
                     ARADTAAGPTPASVATGIPARIATGKPEPVRAAPQVVEPVAPPRRPIPAGMRPLVIAI
                     DAGHGGQDPGSRGPSGAREKDVVLAIARELARQIDATPGLKAFLTRDSDVFIPLPQRA
                     RRASAAKADIFLSIHADAWTSPSAKGAGIFVLSTRGASSQRARLLADKENAADIIGGE
                     QVHKGDRVLANVLLDLTQSGNLKASEEAASHILDHLDDFAHMHKHEVERANFAVLRTS
                     DRMPAMLVETGFISNPEEEGKLRDPAYQRRLAAAILDGVDRYFSNQPPPGTLYAARAQ
                     ARQQAAAGAGSVGAGGAP"
     gene            complement(7876..8328)
                     /gene="tsaE"
                     /locus_tag="PK818_02445"
     CDS             complement(7876..8328)
                     /gene="tsaE"
                     /locus_tag="PK818_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HQY81628.1"
                     /translation="MDPVLLADAEATGALGARLAACLPERAVVHLHGDLGAGKSTLAR
                     ALLRALGVTGTIRSPTYTLVEHYPLAAGGIALHLDLYRIGDVGELEFLGLDGDIRLWL
                     VEWPERGQGALPQADLDIELALEGEGRRCRLRANTAAGRAWLAALGSC"
     gene            complement(8333..9814)
                     /locus_tag="PK818_02450"
     CDS             complement(8333..9814)
                     /locus_tag="PK818_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HQY81629.1"
                     /translation="MPSPLPLHDIVALRALEARAIAASGGDESALMARAGQAGWRCVL
                     QHWPHARRIAVACGPGNNGGDGYVLARHALEAGREVHVLRLQAPGTAVAQRACDDFLA
                     AGGRIVEPVDGLRDAGLIVDALFGIGLSRAPDGAAAALIDAINAHPAPVLALDVPSGV
                     DAANGAAHPAAIVADRTLQFIARHRGLRTGAALDHAGALELATLELPAAVFEGIAPAA
                     LAYRADALPGLFPLRPRDSHKGGNGHVLCIGGDHGSGGAILLAVDAALHCGAGLASVA
                     TRAVHVPALLARRPEAMAHAVEDAAQLAPLLQRADVVAIGPGLGQGAWGQALLDAVLA
                     CDTPRVLDADALNLLAHGGQRLDPHDVITPHPGEAARLLGIDTADVQRDRFAAARELQ
                     ERMGTHVVLKGAGSIVASPGRVPAVVCAGNPGMATGGMGDLLTGCIAALVAQGFAAGD
                     AAVAGALLHAGAGDAAVRDEGERGLLPSDLLPWLRRLANPMRR"
     gene            9850..10911
                     /gene="queG"
                     /locus_tag="PK818_02455"
     CDS             9850..10911
                     /gene="queG"
                     /locus_tag="PK818_02455"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HQY81630.1"
                     /translation="MPPATPDHAALAERIRALAHAAGFQRVGISGVELADDETHLRDW
                     LQQGLHGSMEWMARHGDKRARPAELVPDTLRVVSVGMDYGQDADAAWANLADGERAYV
                     ARYALGRDYHKLMRNRLQALADDVAREIGPFGHRVFVDSAPVLERALARNAGLGWIGK
                     HTCLIDKDGGSWFFLGELFVDLPLPVDPPAGAHCGTCMRCIDVCPTRAITAPYRLDAR
                     RCISYLTIEHEGSIDEALRPLVGNRIFGCDDCQLVCPWNKFARRTDEPDFRTRNNLDQ
                     ATLAELFAWDEDEFLRRTEGSAIRRSGHERWLRNIAVALGNAPGTDAVIEALRSRREH
                     PSPVVREHVEWALSRHRSS"
     gene            complement(11056..12567)
                     /gene="pgi"
                     /locus_tag="PK818_02460"
     CDS             complement(11056..12567)
                     /gene="pgi"
                     /locus_tag="PK818_02460"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HQY81631.1"
                     /translation="MNRLAGLDAHAQRLAGTSLAALIEADPARARDFALRVGPLYANF
                     ARQRYDRIALDALFALAAEADLAGAMQRQLDGEIVNSTEGRAALHTALRGGLSLAAAG
                     PHAQARRARERMQALVASLQASAVTDVVSVGIGGSDLGPRLVVDALAPANARLRVHFI
                     SNVDGNAARRVLAPLDPARTAAIVISKTFGTQETLLNGRIVRDWLGEGAAYHGGRLYA
                     VSANVERAGDFGIPPERILPMWDWVGGRYSLWSAVGFPIALAIGMEAFEALLDGAAAM
                     DAHANEAPLRENLAVLHALTAVWNRNGLHAATQAVLPYDDRLRLLPNYLQQLVMESLG
                     KSVRMDGSDVGLHTVPVWWGGAGTDTQHSFFQALHQGTQAVPADFIGVVRAEHGHADN
                     HMALLANLLAQVEALANGQDADDPHRRYPGDRPSTLLLLDALTPRSLGMLVALYEHSV
                     YLQSVLWGINAFDQFGVELGKQVASRLLPALQGEAEADDAVTRELLRELNQRR"
     gene            complement(12564..12944)
                     /locus_tag="PK818_02465"
     CDS             complement(12564..12944)
                     /locus_tag="PK818_02465"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HQY81632.1"
                     /translation="MQLTLLKAKIHRASVTHAELHYEGSCAIDGRLLDISGIREYERI
                     EIYNVNNGERFATYAIRGEEGSGVISVNGAAAHKAQPGDLVIICAYGHCDEAEAAKFK
                     PTLVYVDRDNRLTHTNQSMPKQAA"
     gene            complement(13049..13909)
                     /gene="panC"
                     /locus_tag="PK818_02470"
     CDS             complement(13049..13909)
                     /gene="panC"
                     /locus_tag="PK818_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HQY81633.1"
                     /translation="MSGIVLLHDLDGLRAQVRGWKRQGLRVGFVPTMGNLHEGHFSLV
                     RLAREHADRVVASVFVNPTQFGPNEDFSRYPRTPGQDAAGLQAAGCDALWMPSVDAMY
                     PYGAGNAVRIHVPGITDTLDGAHRPGHFDGVATVVARLFNQVQPEVAVFGRKDYQQLA
                     VIRYLARDLAFPLEIVAGDTRREDDGLAMSSRNQYLSAEERAIAPAIHRTLLAMRDAI
                     RAGTPRHQVEADAEAALRAAGFAADYAVVRCPDFSTPDADGAGAERVALVAARLGRTR
                     LIDNLEFDAG"
     gene            complement(13906..14715)
                     /gene="panB"
                     /locus_tag="PK818_02475"
     CDS             complement(13906..14715)
                     /gene="panB"
                     /locus_tag="PK818_02475"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HQY81634.1"
                     /translation="MSELQKTWTVPMLARARADGRKLVMLTCYDASFARVLDDNGCDL
                     ILVGDSLGMVVQGHASTLPVRVEDIAYHTAAVARGARRALKIADFPFGSDGSPQEAHA
                     AAVRFIQAGASMVKLEGAGHKLETIRYLVEREIPVCSHLGLTPQSVHRFGGFKVQGRE
                     DAAAARLCEEALAVADAGAALLVLEGVPAPLATRITAASPIPTIGIGAGAGCDGQVLV
                     LHDLLGLDTGHRRPKFVKDFLADGGSVAGAIRAFAAAVRSGAFPAAEHSYE"
     gene            complement(14739..15224)
                     /gene="folK"
                     /locus_tag="PK818_02480"
     CDS             complement(14739..15224)
                     /gene="folK"
                     /locus_tag="PK818_02480"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017169088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HQY81635.1"
                     /translation="MTLAAVGLGANLGAAACTLEQAFAELGRLPRSRLLRASRLYRTR
                     AWGRTDQPDFINAVALVETELAAPDLLQALLAIERGFGRERPAGERWGPRTLDLDLLL
                     FGDAVIDQPGLRVPHPHLHERAFALLPLAEIAPELRIPGRGSVAAHAAGMAADGIEAL
                     P"
     gene            complement(15221..16606)
                     /gene="pcnB"
                     /locus_tag="PK818_02485"
     CDS             complement(15221..16606)
                     /gene="pcnB"
                     /locus_tag="PK818_02485"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HQY81636.1"
                     /translation="MTDTAASTSPTPAGLHVIPRDVHGISRKQMSPNALRVLYRLREA
                     GFGGYLVGGAVRDLLVGGAPKDYDVATDATPEQVRALFRNCRLIGRRFRLAHVVYGRE
                     IIEVATFRANGGDDEGDRLTDDGGRLLRDNIYGSIEDDAIRRDFTANALYYAIEDFSV
                     RDYTGGYADVQARLLRLIGEPEARYREDPVRMLRAARLAAKLGFTIEAATAEPLPRLA
                     PLLAEAAPARLFEETLKLFLSGHAVASFELLEATGLLPALLPETAQALASNKSGALRR
                     MLVQGLRNTDARVAAEESVSPAFLYAVLLWPAYCRALALLQAQGVHAVEAQRRAADRV
                     SLHQVARTALPRRFSLPMQEIWLLQPRFALRQRKRVFRLLAHPRFRAAFDFLELRLTA
                     SDSHAGDVAFWREAQLHPAQAVDASEHAAGDADDPHEADPADAGAADDGGAPRKRRRR
                     RRNGAAAQAPA"
     gene            16751..16826
                     /locus_tag="PK818_02490"
     tRNA            16751..16826
                     /locus_tag="PK818_02490"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16785..16787,aa:Glu,seq:ctc)
     gene            16904..17227
                     /locus_tag="PK818_02495"
     CDS             16904..17227
                     /locus_tag="PK818_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HQY81637.1"
                     /translation="MPFVVTENCIKCKHTDCVEVCPVDCFHEGPNFLVIDPDECIDCT
                     LCEPECPVGAIYPEEDVPAGQEVFVALNAELAKQWPVLATRKDALPDAAEWDGKPGKL
                     PLLER"
     gene            complement(17311..17829)
                     /locus_tag="PK818_02500"
     CDS             complement(17311..17829)
                     /locus_tag="PK818_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81638.1"
                     /translation="MPRSPQAFRFAAPLSAIVLVAAVSGCSMFGEKDELYTQSTESRP
                     LEVPPDLDRPSADRAMSLPATGGAVSASAMNNAGGGSAAPIGFNASGDRDAIFAKVGE
                     VLGATAGVTVVSRAPILGTYDLDYMDAKFLVRVTKVGDGAYVSAVDPRGLPPTGEAPV
                     KLVAALKAAIAP"
     gene            complement(17842..18744)
                     /gene="dapA"
                     /locus_tag="PK818_02505"
     CDS             complement(17842..18744)
                     /gene="dapA"
                     /locus_tag="PK818_02505"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HQY81639.1"
                     /translation="MQLSGSITALATPFTATGELDLPAWQRLLQGQLDAGIHGVVVAG
                     STGEASALFEPEFETLLRTAVEQAGGKLPVLAGTGHSNTAKTIQACRRAAALGADAVL
                     VVTPPYVRPTQSGLLAHFRAVADEGGLPVVLYNVPGRTGCDMAAETSAQLSLHPNIVG
                     IKEARADAERMEALLPLRRRGFAVLSGDDPTAVRAMLAGADGIVSVASNVVPNAFRRL
                     CGLARGGDAAGAEEFDARLHALYHFLAVEPNPIPLKALLALNGIGVGLRLPLQPLSSL
                     HAAEAERLHADILQLEQRCRPQAA"
     gene            18917..19477
                     /locus_tag="PK818_02510"
     CDS             18917..19477
                     /locus_tag="PK818_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein R"
                     /protein_id="HQY81640.1"
                     /translation="MTDTTARPAPNENHLLINAYSTHPQSPLLAVTRRIADSGCNLVD
                     TRLSTVGRDVSVTALATGSWDAVAKLETMMAKLEREEGLKLVWYRTGPKPVQSSLLPY
                     VVEVIASDKPGILFQLADFFDRQGITIESLHCSRYRAMQTGADMFSAQLTIGVPSDMH
                     IAALRDDFLEFCDHLNLDAIMDPMKY"
     gene            19477..19947
                     /locus_tag="PK818_02515"
     CDS             19477..19947
                     /locus_tag="PK818_02515"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQY81641.1"
                     /translation="MPETGDRVPDLPLALSSGKAATLGDYAGKWLVLYFYPKDSTPAC
                     TAEGQDFNALLPDFKRAGAQVLGVSRDSLKSHQNFCAKQGFKFDLASDADERICNAFG
                     VIQPKKLYGRDYIGIVRSTFLVDPEGVIRQSWQPVKVPGHAQAVLDALKAANRK"
     gene            20069..>21366
                     /locus_tag="PK818_02520"
     CDS             20069..>21366
                     /locus_tag="PK818_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010365742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQY81642.1"
                     /translation="MTRSKRIYVLDTNVLMHDPTALFKFEEHDVYLPMQVMEELDNGK
                     KGTSEASRNARQVSRFLNELIEAHGGTDVHHGVALARPKGLQLRGADSVGKLRFQTDN
                     FDAGKRFGAVVPDNAILGAILALKESDPGAPVVFVSKDINLRIKASIAGIASEDYEND
                     RALDDFSLLYTGATALPVDFWERHGKDLKSWTDKGRTYYEIDQGDGDDWYPNQFLYLP
                     GDEEAEMKVAKVTDARVTLQIVDDFRHSQHAVWGITARNREQNFALNALMDPEIDFVT
                     LLGTAGTGKTLLALAAGLAQTMDAQRYREIIMTRATVSVGEDIGFLPGTEEEKMTPWM
                     GALTDNLEVLTHNQEGGAWGRAATNDLLASRIKIRSMNFMRGRTFLSRYLILDEAQNL
                     TPKQMKTLVTRAGPGTKIVCLGNVEQIDTPYLTETTSGLT"
BASE COUNT         3223 a         7686 c         7346 g         3111 t
ORIGIN      
        1 cacttcgtgt gcgccgacga cacccacggc acgccgatca tgctggccgc cgagaaggcc
       61 gggaccacac cggaatcctt catcgcagcc gtccaggcag gccacgagcg cgacttcgcc
      121 gccttcggcg tggccttcga ccactacgac tccaccaact cggccgccaa ccgcgcgctg
      181 accgaacaga tctacgcgcg cctcgacgcc ggcggccaca tcgcccgccg cagcgtggcg
      241 cagttcttcg atccggccaa gggcatgttc ctgcccgatc gctacatcaa gggcacctgc
      301 cccaactgca aggccgccga ccagtacggc gacaactgcg aagtgtgcgg cgccacctat
      361 ggccccaccg acctgatcga gccgaagtcg gtggtgtccg gcgccacccc cgagatgcgc
      421 gattccgagc actacttctt cgaggttggc cgcttcgagg ccttcctgcg cgagtggctg
      481 gccagcgaca ccctgctgcc gggggtgaag gccaagctga tggaatggct ggacgccgac
      541 ggcggcctgc gcgcctggga catctcgcgc gacgcgccgt acttcggctt cgagatcccg
      601 aacgcgcccg gcaagtattt ctacgtctgg ctggacgcgc cgatcggcta cctgtccagc
      661 ttccggaacc tgtgcgcgaa gaccggcatc gacttcgacg ccttcctgaa gcccggcagc
      721 gacgccgagc tgcaccactt catcggcaag gacatcgtca acttccacgg cctgttctgg
      781 ccggcggtgc tgcacggcgc cggcatgcgc acgcccaccc gcctgcacgt caacggctac
      841 ctgaccgtgg acggcgccaa gatgtcgaag tcgcgcggta ccttcgtgat ggcgcgcact
      901 tacctcgatg ccgggctgga cccggaggcg ctgcgctatt acttcgcctg caagacctcg
      961 ggcggcgtgg acgacctgga cctcaacctg gccgatttcg tggcgcgggt gaacagcgac
     1021 gtggtcggca agttcgtcaa catcgccagc cgctgcgccg gcttcatcga caagcgcttc
     1081 gatggccggc tggccgctgc cctgccggat ccggcgcagt accagcgttt cgtggaggcg
     1141 ctgggcggga tcgccgatgc ctacgcccgc aacgaggccg ccaccgcgct gcgcctggca
     1201 atgaccctgg ccgatgaagc caacaagtac atcgacgaac acaagccgtg ggtgctggcc
     1261 aagcaggacg gtgccgacgc gcagctgcac gcggtctgca cgcaggcgct gaacctgttc
     1321 cgggtgctgg cggtcgcgct gaagccggta ctgccacgca ccaccgcgca ggccgacgcc
     1381 ttcctggacg cacccgttgc cggctgggac gacatcacgg cgccgctgct ggcgcgccgc
     1441 atccagccct acgccccgct gttcacccgc atcgacccca agcacatcca gaccatgatc
     1501 gacgccagca aggacaccct cgccgcccct gcaccaaccg cggccgctgc gccggccaag
     1561 gccgcacccg cggatgcgac gccagcgccg aaggccggga cggacaccgc gcagatcggc
     1621 atcgacgact tcgccaagct cgacctgcgc atcggcaagg tgctggaatg cggcttcgtg
     1681 gacggttccg acaagctgct gcgcttcctg ctggatgcag gcgaccttgg ccagcggcag
     1741 atcttctccg gcatccgcgc cagctacgcc gagcctgaaa aactggtcgg ccgcagcgtg
     1801 gtgttcatcg ccaacctggc cccgcgcaag atgcgcttcg gcaccagcga gggaatgatc
     1861 ctgtccgcgg ggttcgacgg tggcagcctg tcgctgctgg acgccgacga aggtgcgctg
     1921 cccggcatgc cggtgcgctg aagaccccgg gcgacgttcg cgtgttgccg cgtctgctgc
     1981 cgcgaaatca aggaggagat gccgaccgca ggccggcatc gggggacatt cgcggcagca
     2041 ggcgcggcaa cgcgcgagcg gcgcatgcga tatgaccgcg ttaccgacaa cggacctgat
     2101 cgaacgcctc gaccgcatac ttccgcagac ccagtgcggt caatgcggtt acgacggttg
     2161 ccgtccatac gcggaagcga tggcgcgcgg cgaggccggc atcgaccatt gcccgcccgg
     2221 cggcgatgcg ggcgcacgcg cgctggcgaa gctgctcggc gtggacgcca ggccctacga
     2281 ccgcagccgc ggcatccgca agccggcgca gttggccctg atcgtggaag ccgactgcat
     2341 cggctgcacc aagtgcatcc aggcctgccc ggtcgacgcc atcgtcggtg cgccgaagtt
     2401 catgcacacg gtggtcgacg cgctgtgcac cggctgcgaa ctgtgcgtgc cggcctgccc
     2461 ggtggactgc atcgtgatgc tgccgccgta gcgcgaaccg gcccgcttgg cggcgggatc
     2521 ggccggtgtt cgcgcagcgg catttcgagc cacgaagcga ctgcctgccg gccccgccgc
     2581 ccggccgatc aatgcacctg ctgcagtgac gtcccttcgc aaccgctgca cacgcgctcg
     2641 actacatagc ccttcgcgcg cagcttcgag accagcccgt cattgcccag caggtgcagg
     2701 ctgccgacca ccaccagggt gtcgtcgacc gtcgcctccg ccagccgcgc ctcgaccttg
     2761 ggcaaccacg cgttgttgcg gtcgatgtcc agcaaccggt aggactgcgg ggacttgcgc
     2821 gccatctccg cgcgcatctc gctgtccagc ttcgccgcgt cgccgctgcg ccaccagcca
     2881 tgcatgtcct gcatctgctg catcgcgtgc cgcgggtcgt cgaggaattc cgcgagtccc
     2941 tgcgcctgct ccgcgtgcga taccgaatcc atcgcccgca tctggtcctc gatggtctcc
     3001 agcccggctg caggcttgcc cgcctccgcc gcgcgcgcca tcaggtggcg atccatcccg
     3061 agttcggccc tgaagcccat cgcatgggtg agccccagca ccaggctcat gctcaccatc
     3121 cacggctcgg tttgctcgaa cgcctgcacc gagccaccgc ttgtcgccag cagggtcgcc
     3181 agcttttcgt gggtggcctt cggcagcacc gtgcccaggc tcttgccgga gtcgtaggcc
     3241 gcgtatttct ggatggtagc cagcgtttcc ggcgcggaca tctcgcgcgg gtcgacctcg
     3301 aacagcagcg attccgcgtc ctcgaacgcc gcgtcgattt ccttcggcaa cgggtagtcg
     3361 tcgggcttga gcaggtggaa ggaaccgagc aggtagacgc tgttgtcggc gtcggagacc
     3421 ttccacagca acgcatggcg tgccgcctgc gcggtcggtg cggcgaccgc ctgtggctgt
     3481 tcggcaaatg cggcgggcac ggccagcgcg gcagccaatg cgaggcaaag caggcgcgat
     3541 tgcaaggcgg tcatccttcg gtgggcggtt tcggggccgc gcgcttgcgg tagtcgcgct
     3601 cgcccgcctc gaccgccagg tccagccggt tttccggcgg cggcagcggg caggtggcga
     3661 acgcggtgaa cgcgcacggc gggttgtagg actggttgaa gtcgatcagg acccgcccct
     3721 gcgcgtccgg cttcggcgcg tcgatgaagc gcccggcgcc atagctgccg cggccattgg
     3781 tgcggtcggc gaataccagg aacagggtgc cctcgccctg gtccagcgcc tccagccgat
     3841 acgtcttgcc gccgcgctcg aattcgaccg cgccggggtt cgggacctcg tcggtggtgc
     3901 cgatgatgtt ggcgatcggc agggtcttgc ccgcggcatg cggcacgaac cgcgcagcca
     3961 cctgccagtc cctgccgccc ggccagtact cgagcccggt gaactgcagg cggctttcgg
     4021 catccgcgtg cttgacccgc aacgccagcc ggccgccgcg ttcgatcacc gtcgccagcc
     4081 ccttgccacc atcgaacacc agtttgctgg ggccggcggg gtccgtgtcg atgcgcagcg
     4141 tgctggcgcc aagcccgggt tcgccgtcga ccatgaccac cgtgtccggg acgaacccga
     4201 ccttgtcgcc gcgcacggtg aaaacgccca ggtgttccgg gccgaccgcc agccggatgc
     4261 cgttgtcggt gtcgctgccg acccgatgcg cgcccggatc cagccagtgc aggccggtca
     4321 ggctggtcca gccgtcgggc cgggtgaggt cggcgacgcg tccggcgcgc caggcctgct
     4381 gccgcgcctg gaacgcggct tcttccgcac gcgcctggcg ggacgcgacc ggcgcctgcg
     4441 ccggctggca cgcagccact ccagccgcga tcacggcagc aagcaaggtc agcgtccgcg
     4501 caggaaccat cgatcgatct ccgccaggtt gaaccgggtc caggtgggac gcccatgatt
     4561 gcattgcccg gaacgttcgg tcgcctccat ctcgcgcagc agcgcgttca tttcgggaag
     4621 ggtcagcctg cgattggcgc gcaccgcccc atggcaggcc atggtggcca gcagtgcgtc
     4681 gcgggtctcg gccacccggc gggtgctgcc gtgttcgcgc aggtcggtga gcacgtcgcg
     4741 cagcagcgcc tccacgtcgc cgtgcgcaag cagcgccggc acgcagcgca gcagcagcga
     4801 ctgcgggccg ctgcgctgca cctcgaaacc cagttgcgcc aacgtgtcgg cctctcgctc
     4861 ggccacgtcg gcctcgcgtt cggacaccgc aatcgcactc gggaccagca gcggctgggt
     4921 gcgcaggccg gcgccgtcgt gcgcggcctt cagcctttcg tagccgatgc gctcgtgggc
     4981 ggcatgcatg tcgaccacga ttagcccatc cgcgttctcg gagaggatgt agatgccgtg
     5041 caactgcgcg atcgcgaatc ccaagggtgg gtgagcaggt gcgtccagcg tctgcgcacg
     5101 cccagccagg gatggctggg cggggctatc cgaagccggc catggttcgc catggatggc
     5161 cgcgtgatcc gtgccgtaca gcgcggcata cgacgctggt gcgtcgccca cccgcaggcc
     5221 catcgacgcc tgctgcggtg gcggcatcga ccaggatggc gatggtgcgt gctgctgcat
     5281 cggcactccg gcgctgcccg cgcgcgtctc cgccagcgca gcgtgcaggg tgcgatagac
     5341 gaagtcgtgg accagccgcg attcgcggaa gcgcacctcg tgcttggccg gatgcacgtt
     5401 gacgtcgacg ccacgcgggt cgagttcgag gaacagcacg taggccggat gacggccgtg
     5461 gaacagcacg tcgctgtacg cctgccgcac cgcgtgggcg atgttgcggt cgcgcacgct
     5521 gcggccgttg acgtagaggt actgctggtc ggcgctggcg cggttgtagg ccggctgcgc
     5581 gatccagcca tgcaggcgca ggcctgcacc ggcgtggtcc acgcgcagcg cgttgcgcgc
     5641 gaagtcctcg cccagcgctt cgtgcaggcg cacgtcactg agcaggtggc cctcgccctt
     5701 ccagcgccgc gacggcttgc cgttgtggct gacccggatt tccacgtccg gccgcgccag
     5761 cgccagcgtg cgcagccatt cttcgatgtg cgacagctcg gtgcgctcgg cgcgcaggaa
     5821 cttgcgccgc gccggcacgt tgtagaacag gtcgcgcact tccaccgtgg tgccgggcgg
     5881 gtgcgcctgc ggcaccacgt cgcccggctt gccgccgtcg atgcgcagct ccgcgccgtg
     5941 ctgcgcatcg tgcctgcgcg atgcgaggcg gaagcggctg accgaggcca ccgacggcag
     6001 cgcctcgccg cggaagccga gcgtggccac cgcttccaga tcgtcgatgg aagcgatctt
     6061 gctggtggcg tggcggctga ccgccagcgg caattcctcc ggcgcgatgc cgccgccgtc
     6121 gtcgcggatc cggatcaggc gcacgccgcc ttcttccagg tcgatgtcga tgcgttgcgc
     6181 gccggcgtcc agcgcgtttt cgaccagttc cttgaccacc gaggcaggcc gctcgacgac
     6241 ttcgccggcg gcgatctggt tgatcagggt atcgggaagt tggcggatgc tcacccgcca
     6301 agtctagcct gcgagaccta cggagccccg cccgcgccga cactgccagc gccggccgcg
     6361 gcttgttgcc gtgcctgcgc acgcgccgcg tacagcgtgc ccggcggcgg ctggttggag
     6421 aaatagcgat ccacgccatc caggatggcg gcggcaaggc ggcgctggta ggccgggtcg
     6481 cgcagcttgc cttcctcttc cgggttggag atgaagccgg tttccaccag catcgccggc
     6541 atccggtccg aggtccgcag caccgcgaag ttggcgcgtt cgacctcgtg cttgtgcatg
     6601 tgcgcgaaat cgtcgaggtg gtcgaggatg tgcgatgcgg cttcctcgga agccttcagg
     6661 ttgccgctct gggtgaggtc cagcagcacg ttggcgagca cccggtcgcc cttgtgcacc
     6721 tgctcgccgc cgatgatgtc ggcggcgttt tccttgtcgg ccagcaggcg cgcgcgctgc
     6781 gaggacgcgc cgcgggtcga cagcacgaag atgccggcgc ccttcgcgct cgggctggtc
     6841 caggcgtcgg cgtggatcga caggaagatg tcggccttgg ccgcgctggc gcggcgcgcg
     6901 cgctgcggca acgggatgaa cacgtcgctg tcgcgggtca ggaacgcctt caggccgggg
     6961 gttgcatcaa tctggcgcgc gagttcgcgg gcgatcgcca gcaccacgtc cttctcgcgg
     7021 gcaccgctgg gcccgcgcga tcccggatcc tggccgccat ggccggcgtc gatcgcgatc
     7081 accagcgggc gcatgccggc cgggatcggg cgacgcggcg gcgcaaccgg ttcgaccacc
     7141 tgcggcgcag cccgcacggg ttccggcttg ccggtggcga tccgggccgg gatgccggtg
     7201 gcgactgatg cgggcgtcgg gccggcagcg gtgtcggcgc gtgcgccggt cgctgccggc
     7261 gcgaccggca cgttcgcgga tgcgctggcg atcgccgcga tcgggtcgct ggccgcggcc
     7321 accgccgttg cctcgggctt ggcagggggc acggcatcgg caacgccatc gccaggccat
     7381 tccagcacca gccgcggacc ctcggtgcct tgctcgatgc gcggcgtgag cacggccacg
     7441 ccgctcgaca ggtcgaacac gatgcgcgcg gtgccgggct ccggctgccc gctgcgcacc
     7501 gccttgacca ccccggccgc acccggaagg ctcaggccac gcccgaggtg gctgcccggg
     7561 aagtcgacga ccaggcggtc ggggttgcgc aactcgatca gcttgtaggc gccttcgcgg
     7621 tccagcagca cctcggcgcg cgttccggtg gccccggtat caacgcgcaa gtgcttgatt
     7681 tcagatgcgt ttgccagatt ccagcagagc gcggccagca gcatggcggc caacagcagt
     7741 tgctcgaagc tgatcccctt gacgcgcatg cccggagtta accacctgac ggcacgcttg
     7801 gcaagggtat ttcccttaat aatccgtaac ctgccgcaac ttcctgcgga acgcgtcaag
     7861 tgctggccgc aggcctcagc aggaccccaa cgcggccagc caggcgcgac cggctgccgt
     7921 gttcgcgcgc agccggcaac gacggccctc gccttccagc gccagttcga tatcaaggtc
     7981 ggcctgcggc aatgccccct gcccgcgttc cggccactcg accagccaca gccggatgtc
     8041 gccgtccagg cccaggaact cgagttcgcc cacatcgccg atccggtaca ggtccaggtg
     8101 cagggcgatg ccgccggccg ccagcgggta gtgctcgacc agggtgtagg tcgggctgcg
     8161 gatggtcccg gtgaccccga gcgcgcgcag cagggcgcgc gccagggtcg acttgccggc
     8221 gccgaggtcg ccgtgcaggt gcaccaccgc gcgctccggc aagcacgccg cgagccgcgc
     8281 gccaagcgcg ccggtcgctt cggcatccgc cagcaaaacc gggtccatgc cgctagcgcc
     8341 gcatcgggtt ggccagccgc cgcagccatg gcagcaggtc gctgggcagc agtccgcgct
     8401 cgccctcgtc gcggaccgcg gcatcgccgg cgccggcatg cagcaatgcc ccggcaaccg
     8461 cggcatcacc cgcggcaaag ccctgcgcga ccagcgcggc gatgcagccg gtcagcaggt
     8521 cgcccatgcc gccggtcgcc atgcccggat tgcccgcgca gaccaccgcc ggcacccggc
     8581 cgggcgaggc gacgatgctg ccggcgccct tcagcaccac gtgcgtcccc atccgttctt
     8641 gcaattcgcg ggcagcagcg aagcggtcgc gctgcacgtc ggcggtgtcg atgccgagca
     8701 gccgcgcggc ctcgcccgga tgcggggtga tcacgtcgtg cgggtccagg cgctggccgc
     8761 cgtgcgcgag caggttgagg gcatcggcat ccagcacgcg cggggtgtcg caggccagca
     8821 ccgcatccag cagggcctgg ccccacgccc cctgccccag tccgggaccg atcgcgacca
     8881 cgtccgcgcg ctgcagcagc ggggccagct gcgcggcgtc ctcgaccgcg tgcgccatcg
     8941 cctccggacg gcgcgccagc agtgccggca cgtgcaccgc gcgggttgcc acgctggcca
     9001 ggcctgcacc gcaatgcagc gcggcgtcga ccgccagcag gatcgcgcca ccgctgccat
     9061 ggtcgccacc aatgcacagc acatggccgt tgccgccctt gtgcgaatcg cgcgggcgca
     9121 gcgggaacag gccgggcaat gcatcggcgc gatacgccag cgctgccggc gcgatgccct
     9181 cgaacaccgc ggcggggagt tccagcgtcg ccagctccag cgcgccggca tgatcgagcg
     9241 ccgcgccggt gcgcaggccg cggtggcggg cgatgaactg cagggtgcgg tcggcaacga
     9301 tcgccgccgg gtgcgcggcg ccgttggccg catcgacgcc gctgggcacg tccagcgcca
     9361 gtaccggcgc cggatgcgcg ttgatcgcat cgatcagggc ggccgcggca ccgtccggtg
     9421 cgcgcgacag gccgatcccg aacagcgcat ccacgatcag tccggcatcc cgcaagccat
     9481 ccacgggctc gacgatgcgg ccaccggcag ccaggaagtc gtcgcaggcc cgttgcgcca
     9541 ccgccgtgcc cggcgcctgc agccgcagca cgtggacttc gcgaccggcc tccagcgcat
     9601 gccgcgccag cacgtagcca tcgccaccgt tgttgccggg cccgcacgcc accgcgatcc
     9661 gccgtgcgtg cggccagtgc tgcagcacgc agcgccagcc ggcctgcccg gcacgcgcca
     9721 tcaacgcgga ttcatcgccg ccggacgcgg cgatcgcgcg cgcctcgagc gcgcgcaggg
     9781 caacgatgtc gtgcagcggc agcggtgaag gcatccggcg attctaagcc gcgcgcctat
     9841 acttcacaca tgccacccgc aacacccgat cacgccgcgc tggccgagcg catccgcgcg
     9901 ctggcgcatg ccgcgggctt ccagcgggtc ggcatttccg gggtggaact ggcggacgac
     9961 gaaacccacc tgcgcgactg gctgcaacag ggcctgcacg gttcgatgga atggatggcg
    10021 cggcacggcg acaagcgcgc gcggccggcc gaactggtgc cggacacgct gcgggtggtc
    10081 tcggtcggga tggactacgg ccaggacgcg gacgccgcct gggcgaacct ggccgatggc
    10141 gagcgcgcct acgtggcgcg ctatgcgctg gggcgcgact accacaagct gatgcgcaac
    10201 cgcctgcagg cgctggcgga cgacgtggcg cgggaaatcg gcccgttcgg ccaccgcgtg
    10261 ttcgtggatt ccgcgccggt gctggaacgc gcgctggcgc gcaacgccgg gctcggctgg
    10321 atcggcaagc acacctgcct gatcgacaag gacggcggca gctggttctt cctcggcgag
    10381 ctgttcgtcg acctgccgct gccggtcgac ccgcccgccg gcgcccattg cggcacctgc
    10441 atgcgctgca tcgatgtctg cccgacccgg gcgatcaccg cgccgtatcg gctggatgcg
    10501 cggcgctgca tttcctatct caccatcgag cacgaaggca gcatcgacga ggcgctgcgc
    10561 ccgctggtcg gcaaccgcat cttcggttgc gacgactgcc agctggtgtg tccgtggaac
    10621 aagttcgcgc gccgcaccga cgagccggat ttccgcaccc gcaacaacct cgaccaggcg
    10681 accctggcgg agctgttcgc ctgggacgaa gacgaattcc tgcgccgcac cgagggttcg
    10741 gcgatccgcc gcagcgggca cgagcgctgg ctgcgcaaca tcgcggtggc gctgggcaat
    10801 gcgccgggga cggatgcggt gatcgaagcc ttgcggtcgc gccgcgagca tccctcgccg
    10861 gtggtgcgcg agcatgtgga gtgggcgctg agccggcacc gctcttcctg acaacggcgg
    10921 aaccacctcg caggacggat ggccccgggt gtcttcggga ccttcggcga catggatgtc
    10981 gccgatgagc ctacatggac gtacttgcgg cgtgtcccga agacacccgg ggccagccgg
    11041 ccaatcggaa tccagtcagc gtcgctggtt caattcgcgc aacagctcgc gcgtcaccgc
    11101 atcgtccgcc tcggcttcgc cctgcagcgc cggcagcagg cggcttgcga cctgcttgcc
    11161 gagttcgacc ccgaactggt cgaaggcatt gatcccccac agcaccgact gcaggtacac
    11221 gctgtgctcg taaagggcga ccagcatgcc cagcgatcgc ggggtcagcg catccagcag
    11281 cagcagggtg ctggggcggt cgccggggta acggcggtgc ggatcgtctg catcctggcc
    11341 gttcgccagt gcctccacct gcgccagcag gttggccagc agcgccatgt ggttgtcggc
    11401 gtggccatgc tcggcgcgga ccaccccgat gaaatcggcg ggcaccgcct gggtgccctg
    11461 gtgcagggcc tggaagaagc tgtgctgggt atcggtaccg gcaccgcccc accacaccgg
    11521 caccgtgtgc aggccgacgt cgctgccgtc catccgcacc gacttgccca ggctttccat
    11581 caccagctgc tgcaggtagt tcggcagcag ccgcaggcgg tcgtcgtacg gcagcaccgc
    11641 ctgggtggcc gcgtgcaggc cgttgcggtt ccagaccgcg gtcagcgcgt gcagcaccgc
    11701 caggttttcc cgcagcgggg cctcgttggc gtgcgcgtcc atcgccgccg cgccatccag
    11761 cagcgcttcg aaggcctcca tgccgatcgc cagcgcgatc gggaagccca ccgccgacca
    11821 caacgagtag cggccgccga cccagtccca catcggcagg atccgctccg gcgggatgcc
    11881 gaaatcgccg gcgcgctcca cgttcgcgct gaccgcgtac aggcggccac catggtaggc
    11941 ggcgccctcg cccagccagt cgcggacgat gcggccgttg agcagggtct cctgggtgcc
    12001 gaaggtcttg gagatcacga tcgccgcggt gcgcgccgga tccagcggcg ccagcacccg
    12061 ccgcgcggca ttgccgtcga cgttggaaat gaagtgcacg cgcaggcgcg cgttcgccgg
    12121 cgccagcgca tccaccacca gccgtggccc gaggtcggac ccgccgatgc cgacgctgac
    12181 cacgtcggtg accgccgacg cctgcaggct tgcgaccagc gcctgcatgc gctcgcgggc
    12241 ccgccgcgcc tgcgcatgcg ggccggcggc cgccagcgac agcccgccgc gcaacgccgt
    12301 gtgcagcgcg gcacggcctt cggtcgaatt gacgatctcg ccatccagct gccgctgcat
    12361 cgccccggcg aggtcggcct ccgccgcaag cgcgaacagc gcgtcgagcg cgatgcggtc
    12421 gtagcgctgg cgggcgaagt tggcgtacag cggccccacc cgcagggcga aatcgcgcgc
    12481 gcgcgccggg tcggcttcga tcagcgcggc gagcgaggtg ccggccagcc gctgcgcatg
    12541 cgcatcgagg ccggccaggc ggttcatgcg gcctgcttcg gcatcgactg gttggtgtgg
    12601 gtcaggcggt tgtcgcggtc cacgtacacc agcgtcggct tgaacttcgc cgcctcggcc
    12661 tcgtcgcaat ggccgtacgc gcagatgatc accaggtcgc cgggctgcgc cttgtgcgcg
    12721 gccgcgccgt tgaccgagat caccccgctg ccttcctcgc cgcggatcgc gtaggtcgcg
    12781 aagcgctcgc cgttgttgac gttgtagatc tcgatgcgct cgtattcgcg gatgccggag
    12841 atgtccagca ggcgcccgtc gatcgcgcac gagccttcgt aatgcagctc cgcgtgggtc
    12901 acgctggcgc ggtggatctt ggccttgagc agggtcagtt gcatggtttc ggctccggcg
    12961 gcatgccgcc aacgatcggc ccgggcagcg cccggcacgg cgcagtatag gggtctgttt
    13021 ccgcactgca acatcaccgc ggcgacgttc agccggcgtc gaactccagg ttgtcgatca
    13081 accgcgtccg gcccagccgg gccgcgacca gcgcgacccg ctccgcgccc gcgccatcgg
    13141 cgtccggcgt actgaaatcg gggcaccgga ccaccgcgta atccgccgcg aaccccgccg
    13201 cgcgcagggc cgcttcggcg tccgcctcga cctggtggcg cggcgtgccg gcacggatcg
    13261 cgtcgcgcat ggccagcagg gttcgatgga tggccggcgc gatcgcgcgc tcttccgccg
    13321 acaggtactg gttgcgcgaa ctcatcgcca ggccatcgtc ttcgcggcgg gtgtcgccgg
    13381 ccacgatctc cagcgggaac gcaaggtcgc gggccaggta acggatcacc gccagctgct
    13441 ggtaatcctt gcggccgaac accgccacct ccggctgcac ctggttgaac aggcgcgcga
    13501 ccacggtggc gacgccgtcg aaatggccgg ggcgatgcgc gccgtcgagg gtgtcggtga
    13561 tgccgggaac gtggatgcgc accgcgttgc ctgcgccgta cggatacatc gcgtcgaccg
    13621 atggcatcca cagcgcgtcg caacccgccg cctgcaggcc ggcggcatcc tgcccgggcg
    13681 tgcgtgggta gcgggagaaa tcctcgttcg ggccgaactg ggtcgggttc acgaacacgc
    13741 tggcgaccac ccggtcggcg tgctcgcgcg ccaggcggac cagcgagaag tggccttcat
    13801 gcaggttgcc catggtcggc acgaagccga cgcgcaggcc ctgccgcttc catccgcgga
    13861 cctgcgcgcg caggccgtcg aggtcgtgca ggaggacgat cccgctcatt cgtagctgtg
    13921 ctcggcggcc gggaacgcac cgctgcgcac ggcagcggcg aaggcgcgga tggcgccggc
    13981 cacggagccg ccgtcggcca ggaaatcctt gacgaacttg ggccggcggt ggccggtgtc
    14041 caggcccagc aggtcgtgca gcaccagcac ctggccgtcg cagccggcgc cggcgccgat
    14101 gccgatggtc gggatcgggc tggcggcggt gatccgggtc gccagcggcg ccggcacgcc
    14161 ttccagcacc agcagggcgg caccggcgtc ggccacggcc agggcctcct cgcacaagcg
    14221 tgcggcggcg gcgtcctcgc ggccctgcac cttgaaaccg ccgaaacggt gcaccgactg
    14281 cggggtcaac ccgaggtggc tgcagaccgg gatctcgcgc tcgaccaggt agcggatggt
    14341 ctcaagcttg tggccggcgc cttccagctt gaccatgctg gcgccggcct ggatgaagcg
    14401 caccgcggct gcatgcgctt cctgcgggga gccatcggaa ccgaacggga aatccgcgat
    14461 cttcagcgcg cgcctggcgc cgcgcgccac cgcggcggtg tggtaggcga tgtcctccac
    14521 ccgcaccggc aaggtggacg cgtgcccctg caccaccatg cccagcgaat cgccgaccag
    14581 gatcaggtcg cagccgttgt cgtcgagcac ccgcgcgaag ctggcgtcgt aacaggtcag
    14641 catcaccagc ttgcggccgt cggcgcgcgc gcgcgccagc atcggcaccg tccaggtctt
    14701 ctgcaattcg ctcatgcctg gctcctgccc gcgggcggct aaggtaacgc ttcgatgccg
    14761 tcggccgcca tgccggcggc atgcgcggcg acgctgccgc gtccgggaat ccgcagctcc
    14821 ggcgcgatct ccgccagtgg cagcagcgcg aacgcgcgtt cgtgcaggtg cggatgcggc
    14881 acccgcaggc ccggctggtc gatcaccgcg tcgccgaaca gcagcaggtc gaggtccagc
    14941 gtgcgcggcc cccaacgctc gccggccggg cgctcgcggc cgaagccgcg ttcgatcgcg
    15001 agcagggcct gcaacaggtc gggcgcggcc agttcggtct cgaccagcgc cactgcattg
    15061 atgaagtccg gctggtcggt gcggccccag gcgcgcgtgc gatacaggcg cgaggcgcgc
    15121 agcagccgcg agcgcggcag ccgccccaat tcggcgaacg cctgctccag cgtgcaggca
    15181 gcggcgccga ggttggcgcc cagcccgact gcggccaggg tcatgccggg gcttgcgcgg
    15241 ccgccccgtt gcgacggcgg cggcggcgct tgcgcggcgc acccccatcg tccgctgcac
    15301 cggcgtcggc tgggtccgcc tcgtgcgggt cgtccgcgtc gcccgccgcg tgttcgctgg
    15361 cgtccacggc ttgcgccgga tgcagttgcg cctcgcgcca gaacgcgacg tcacccgcat
    15421 ggctgtccga tgcggtcagg cgcaactcga ggaaatcgaa tgcggcgcgg aagcgcggat
    15481 gcgcaagcag gcggaacacc cgcttgcgct ggcgcaacgc gaagcgcggc tgcaacagcc
    15541 agatctcctg catcggcagc gagaaccggc gcggcagcgc ggtgcgcgcg acctggtgca
    15601 ggctgacccg gtcggcggcg cgccgctggg cctcgaccgc atgcacgcct tgcgcctgca
    15661 gcagcgccag cgcccggcaa tacgccggcc acagcagcac cgcatacagg aacgcgggtg
    15721 aaaccgattc ctccgccgcg acccgcgcgt cggtgttgcg caggccctgc accagcatcc
    15781 gccgcaatgc gccggacttg ttggaagcca atgcctgcgc ggtttccggc agcagcgccg
    15841 gcagcaaccc ggtcgcctcc agcaactcga agctggcgac cgcatggccg gacaggaaca
    15901 gcttcagggt ttcctcgaac agccgcgccg gcgcggcttc tgccagcagc ggcgccagcc
    15961 gcggcaacgg ttcggcggtg gcggcctcga tcgtgaaccc cagcttcgcg gccagccgcg
    16021 ccgcccgcag catccgcacc ggatcctcgc gatagcgcgc ttccggctcg ccgatcaggc
    16081 gcagcaggcg cgcctgcacg tcggcatagc cgccggtgta gtcgcgtacc gagaagtcct
    16141 cgatcgcgta gtacagcgcg ttcgcggtga agtcgcggcg gatcgcatca tcctcgatgc
    16201 tgccgtagat gttgtcgcgc agcaggcggc cgccatcgtc ggtcaggcgg tcgccctcgt
    16261 cgtcgccgcc gttggcgcgg aacgtcgcca cttcgatgat ctcgcgcccg tagaccacgt
    16321 gggccaggcg gaagcggcgc ccgatcaggc ggcaattgcg gaacagcgcg cggacctgtt
    16381 ccggggtggc gtcggtggcg acgtcgtaat ccttgggcgc gccgccgacc agcaggtcgc
    16441 gcaccgcgcc accgaccagg tagccaccga agccggcctc ccgcagtcgg taaagcacgc
    16501 gcagggcgtt cgggctcatc tgcttgcgcg agatgccatg cacgtcgcgc gggatgacgt
    16561 gcaggccggc gggggtcggg gatgtggatg cggcggtgtc ggtcatgcgt tgcggccgtg
    16621 ccgctgggtt cctgggactg gcgggcgcgt tgccaggggc cgacgcggcc ttctatacta
    16681 gcaagcccgg tgcacggctt cggctgtacc cgggccggcc gtcctgccgg aggggcatcg
    16741 ccccgaccct gctcccatcg tctagaggcc taggacacca ccctctcaag gtggacacac
    16801 gggttcgaat cccgttggga gtaccaatac cgaagcccgc gtagcgatgc gcgggctttt
    16861 tcatgggcgc tacactaccc gcatcgcatc acggatcccg gccatgccct tcgtcgtcac
    16921 cgagaactgc atcaagtgca agcacaccga ctgcgtggaa gtgtgcccgg ttgactgctt
    16981 ccacgaaggc ccgaacttcc tggtgatcga cccggacgag tgcatcgact gcaccctctg
    17041 cgaaccggag tgcccggtcg gcgcgatcta tccggaggag gacgtgcccg ccggccagga
    17101 ggtcttcgtc gcgctcaacg ccgagctagc gaagcagtgg ccggtactcg ccacccgcaa
    17161 ggatgcgctg ccggatgccg ccgaatggga cggcaagccc ggcaagctgc cgctgctgga
    17221 gcgctgatcc gcgcccggcc cgcaacggcc aaaccatgaa aaaaggcgcc acgggggcgc
    17281 ccttctccaa gcatggcggt ggacgcctgc ttacggggcg atggccgcct tcagcgccgc
    17341 gaccagcttg accggcgcct cgccggtcgg cggcaggccg cgcggatcga ccgccgacac
    17401 ataggcaccg tcgcctacct tggtcacccg caccaggaac ttggcgtcca tgtagtcgag
    17461 gtcataggta ccgaggatcg gcgcgcggct caccacggtc acgcccgcgg tggcgcccag
    17521 cacctcgccg accttcgcga agatcgcatc gcgatcgccg ctggcattga agccgatcgg
    17581 cgccgcgctg ccgccgccgg cgttgttcat ggccgacgcc gagaccgcgc caccggtggc
    17641 cggcagcgac atcgcgcgat ccgcgctggg gcggtcgagg tccggcggca cttccagcgg
    17701 ccggctctcg gtgctctggg tgtacagctc gtccttctcg ccgaacatgc tgcagccgga
    17761 caccgccgcg accagcacga ttgcggacag cggtgcggcg aagcggaagg cttgggggga
    17821 acggggcatc aggagctcct gtcaggccgc ctgcgggcgg cagcgttgtt cgagttggag
    17881 gatgtccgca tgcaggcgct cggcctcggc ggcatgcagg gatgatagcg gttgcagcgg
    17941 caggcgcagg ccgacgccga ttccgttcag cgccaggagg gctttcagcg ggatcggatt
    18001 gggttcgacg gcgaggaagt ggtagagcgc gtgcaggcgc gcatcgaatt cctccgcacc
    18061 cgccgcgtcg ccgccgcggg ccagcccgca caggcggcgg aaggcattcg ggaccacgtt
    18121 cgaggccacc gagacgatgc cgtcggcgcc ggccagcatc gcccgtaccg cggtcgggtc
    18181 gtcgccgctg agcacggcga acccgcgccg gcgcagcggc agcaacgcct ccatgcgttc
    18241 ggcatcggcg cgcgcttcct tgatgccgac gatgttggga tgcagcgaca gctgggcgct
    18301 ggtttccgcc gccatgtcgc agccggtgcg gcccggcacg ttgtacagga ccaccggcaa
    18361 cccgccctcg tcggcgaccg cgcggaaatg cgcgagcagc ccggactggg tggggcgcac
    18421 gtagggcggg gtcaccacca gcaccgcatc cgcccccagc gccgcggcgc ggcggcaggc
    18481 ctggatggtc ttggcggtat tggagtggcc ggtcccggcc agcaccggca gcttgccacc
    18541 ggcctgctcc actgcggtgc gcagcagggt ttcgaactcg ggctcgaaca gggccgacgc
    18601 ctcgccggtc gatccggcca cgaccacccc gtggatgccg gcatccagct ggccctgcag
    18661 cagtcgctgc caggcgggta ggtcgagctc gccggtcgcc gtgaacggcg tcgccagcgc
    18721 ggtgatgctg ccggaaagtt gcaagggagt gctcgcggct gggcgccagg cggcgtgcgt
    18781 ggcgcaggag gaaggggccg tacgcgtccg atcttacttg cggcgcgaaa gcgccggcaa
    18841 gtatgctggc cgcacgcacg ccggccgcac cgccgccgac ttccagcaat acgcccccat
    18901 cgcttcggac gcccccttga ccgacaccac cgccaggccc gcgccgaacg aaaaccacct
    18961 gctgatcaac gcgtacagca cgcatccgca gtcgcccttg ctcgccgtca cccgccgcat
    19021 cgccgactcc ggctgcaacc tggtcgacac ccgcctgtcc acggtcggcc gcgatgtctc
    19081 ggtgaccgcg ctcgccaccg gttcctggga cgcggtggcc aagctcgaga ccatgatggc
    19141 caagctggag cgcgaggaag gcctgaagct ggtctggtac cgcaccgggc caaagccggt
    19201 gcagtccagc ctgctgccgt acgtggtcga ggtgatcgcc tcggacaagc ccggcatcct
    19261 gttccagctg gcggacttct tcgaccgcca gggcatcacc atcgagagcc tgcactgctc
    19321 gcgctaccgg gcgatgcaga ccggcgccga catgttctcc gcgcagctga ccatcggggt
    19381 cccttcggac atgcacatcg ccgccctgcg cgacgatttc ctcgagttct gcgaccacct
    19441 gaacctcgac gccatcatgg atccgatgaa gtactgatgc ccgagaccgg cgaccgcgtc
    19501 cccgatctcc ccctggccct gtccagcggc aaggccgcga cgctcggcga ttacgccggc
    19561 aagtggctgg tcctgtattt ctacccgaag gattccaccc cggcctgcac cgccgagggc
    19621 caggacttca acgcgctgct gcccgatttc aagcgcgccg gcgcgcaggt gctgggcgtg
    19681 tccagggact cgctgaagtc gcaccagaac ttctgcgcca agcagggctt caagttcgac
    19741 ctcgccagcg atgcggacga acgcatctgc aatgcctttg gcgtgatcca gccgaagaag
    19801 ctctacggcc gcgactacat cggcatcgtc cgcagcacct tcctggtcga ccccgagggc
    19861 gtcatccgcc agtcatggca gccggtgaaa gtccccgggc atgcacaggc cgtgctcgac
    19921 gccctgaagg ccgcgaaccg caagtgaccg cccccgcttc cagccattcc cgcccgacgc
    19981 agcgcgccgg gcgtccctcg tcgcgcgcgc cggcggcgcg caggccgctg caccccacca
    20041 accgctgtcc gaccgcagga atccgagcat gacgcgaagc aagcgcatct acgtcctcga
    20101 taccaacgtg ctgatgcacg acccgaccgc gctgttcaag ttcgaggaac acgatgtgta
    20161 cctgccgatg caggtgatgg aggagctgga caacggcaag aagggcacct ccgaggccag
    20221 ccgcaacgcg cgccaggtca gccgtttcct gaacgagctg atcgaggccc acggcggcac
    20281 cgacgtccac catggggtgg cgctggcgcg gccgaagggg ctgcagctgc gcggtgcgga
    20341 cagcgtcggc aagctgcgct tccagaccga caatttcgat gccggcaagc gcttcggcgc
    20401 ggtggtcccg gacaacgcga tcctcggcgc gatcctggcg ctgaaggaat ccgatcccgg
    20461 cgcgccggtg gtgttcgtgt ccaaggacat caacctgcgg atcaaggcct cgatcgcggg
    20521 catcgccagc gaagactacg agaacgaccg cgcgctggac gatttcagcc tgctctacac
    20581 cggcgccacc gcgctgccgg tggacttctg ggaacgccac ggcaaggacc tgaagtcgtg
    20641 gaccgacaag ggccgcacct actacgagat cgaccaaggc gacggcgacg actggtatcc
    20701 gaaccagttc ctgtacctgc cgggcgacga ggaagcggag atgaaggtgg cgaaggtcac
    20761 cgatgccagg gtgaccctgc agatcgtcga cgatttccgc cacagccagc acgcggtgtg
    20821 gggcatcacc gcgcgcaacc gcgagcagaa cttcgcgctg aacgcgctga tggacccgga
    20881 gatcgacttc gtcaccctgc tcggcaccgc cggcaccggc aagaccctgc tggcgctggc
    20941 cgcgggcctc gcgcagacca tggatgcgca gcgctaccgc gagatcatca tgacccgcgc
    21001 caccgtcagc gtgggcgagg acatcggctt cctgcccggc accgaggaag agaaaatgac
    21061 gccctggatg ggcgccctga ccgacaacct ggaagtgctg acccacaacc aggagggcgg
    21121 cgcctggggc cgcgcggcga ccaacgacct gctggcctcg cggatcaaga tccgctcgat
    21181 gaacttcatg cgcgggcgca cgttcctgtc gcgctacctg atcctggacg aggcgcagaa
    21241 cctcaccccc aagcagatga agaccctggt cacccgcgcc ggccccggca ccaagatcgt
    21301 ctgcctgggc aacgtcgagc agatcgacac gccctacctc accgagacca cctctggcct
    21361 gaccta
//
LOCUS       DAORUK010000020        20770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_2170_length_20770_cov_21.701183, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000020 DAORUK010000000
VERSION     DAORUK010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 20770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20770
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2170_length_20770_cov_21.701183"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PK818_02525"
     CDS             complement(<1..367)
                     /locus_tag="PK818_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008575562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HQY81643.1"
                     /translation="MTAASTERPIDRWFAHYSGDHRNPVNQRIHVVAVPLILWSVVGL
                     LWCIPVVGSWFRPGLWAALAMFAAWMFYYRASRAIGFGMLATFVAMAWLTRWLHDSYG
                     TPGLARLALGVFVLAWIAQF"
     gene            complement(450..1346)
                     /locus_tag="PK818_02530"
     CDS             complement(450..1346)
                     /locus_tag="PK818_02530"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006448471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HQY81644.1"
                     /translation="MATTLLQSDLPGLNLIHRGKVRDVFDLGDGHLLMVATDRLSAFD
                     VVLPDPIPGKGEMLCQISNFWFDKTAHIIANHLTGIDVASVLPAGVDPALYAKRAVVT
                     KKLTPVPVECIARGYIIGSGWKDYQRTGEVSGIRLPAGLQQAQQLPAPIFTPSTKAAV
                     GDHDENIDFATAVDLVGRERAEQVRDATLALYAFARDYAAQRGILLADTKFEFGTDVD
                     GRLYVMDEMFTPDSSRYWPAEQYQVGTSPPSYDKQFVRDYLETLDWNKTAPGPRLPAD
                     VIDRTRAKYAEALQKLAGISID"
     gene            1430..1759
                     /locus_tag="PK818_02535"
     CDS             1430..1759
                     /locus_tag="PK818_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HQY81645.1"
                     /translation="MKRWYGKFFGLIAGWLLLRHPAGGLLGLLIGHAFDADWFKSTRD
                     NPYRVFGLTEEASDAEIDLAYRRLISQYHPDRVAGAAPELQRQAEAKAREINAAYDRI
                     KALRNRK"
     gene            1850..2521
                     /gene="rpe"
                     /locus_tag="PK818_02540"
     CDS             1850..2521
                     /gene="rpe"
                     /locus_tag="PK818_02540"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010379428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HQY81646.1"
                     /translation="MQPTVIAPSILSANFARLGEEVDNVLAAGADWVHFDVMDNHYVP
                     NLTIGPLVCEALRKHGVTAPIDVHLMVEPVDGLIPMFADAGASHISFHPEASRHVHRS
                     VQLIKSKGCQAGLVFNPASSLDVLEVMLDEIDYVLLMSVNPGFGGQSFIASALRQLRR
                     VRELIDASGRPVRLEIDGGVKPDNIADIAAAGADTFVAGSAIFGKPDYAAVIARMKQA
                     VAAVR"
     gene            2808..4298
                     /gene="trpE"
                     /locus_tag="PK818_02545"
     CDS             2808..4298
                     /gene="trpE"
                     /locus_tag="PK818_02545"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008575582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HQY81647.1"
                     /translation="MITHAQFDQHAADGHTLIPVVREVLSDLDTPLSVYLKLADGPHT
                     YLFESVEGGERFGRYSIIGLPARRVYEVRGHALQVREHGEVVETRELDDPLAEVERLR
                     AHYRVPKLDGLPGFSGGLVGWFGFECIEYIEPCLRANPLPDELGTPDILLMLSEELAV
                     FDNLKGRLYLIVHADPRQPQAWARAQRRLDALAHRLRQAGRGYPETLHGNALDEADFV
                     SGFTREGFIEAVHRSKQYIASGDAFQVVLSQRLSVPFRARPVDVYRALRATNPSPYMY
                     FLDTGATQVVGSSPEILVRLQHTVDGGGEVTVRPIAGTRPRGRTHEEDLALEAELLAD
                     PKERAEHLMLIDLGRNDAGRVSEPGTVVVGEQFVIERYSHVMHIVSEVTGRLKQGLSY
                     MDVLRATFPAGTVSGAPKIRALQIIRELEPVKRNIYSGAVGYIGWHGDADTAIAIRTA
                     VIQDGRLHVQAGAGIVYDSDPQAEWDETMNKGRALFRAVAEAAKGL"
     gene            4300..4878
                     /locus_tag="PK818_02550"
     CDS             4300..4878
                     /locus_tag="PK818_02550"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HQY81648.1"
                     /translation="MLLMIDNYDSFTWNLVQYLQSLGAEVRVERNDALTVDEIERLAP
                     ERIVISPGPCTPNEAGVSMAVVERLGATTPILGVCLGHQSIGQVYGGDVVRAGKIMHG
                     KTSRIRHQGKGVFAGLPDGFEATRYHSLVVDKATLPDALEITAWTENDDGSMEAIMGL
                     RHRGHPVEGVQFHPESILTQHGHALLKNFLER"
     gene            4883..5917
                     /gene="trpD"
                     /locus_tag="PK818_02555"
     CDS             4883..5917
                     /gene="trpD"
                     /locus_tag="PK818_02555"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HQY81649.1"
                     /translation="MPFTPQDALQRAIEHREIFFDEMVELMRQVMRGEVSPVMTAAIL
                     TGLRVKKETVGEIAAAATVLREFARPVDVADRTHLVDIVGTGGDGAHTFNISTASMFV
                     VAAAGGKVAKHGNRSVSSKSGSADVLEALGANIELQPGQVAACIERCGIGFMFAPVHH
                     PAMKVVAPVRREMGVRTLFNILGPLTNPANAPGILMGVFHPDLVGIQVRVLQELGAQR
                     ALVVWGRDGMDELSLGAGTLVGELRDGVVREYELHPEDFGIAMAHSRNLRVGNATESM
                     DLLRQALDNRECLPREIVAYNAGAALYAAGVASDIGDGILRARNAIASGAAAAKLDEF
                     VATTQALAGA"
     gene            5949..7121
                     /gene="iadA"
                     /locus_tag="PK818_02560"
     CDS             5949..7121
                     /gene="iadA"
                     /locus_tag="PK818_02560"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HQY81650.1"
                     /translation="MTAPLLLLRNADVYAPEPLGRRHLLLGGGKVLWMGGSGPGLPEA
                     LGVETIDLEGARLLPGFIDGHVHASGGGGEAGFASRVPAPLLSRYTSAGVTTVIGLLG
                     TDDVSRGTSELLSHVYALREQGLGAWAYCGGYHIPPTTLTGSVRGDIVFSDALIGVGE
                     VAISDHRSSQPTLDDVLRLASEAHVAGLMTGKAGIVHLHLGDGPRGLELVRQALDTSE
                     LPPRVFQPTHVNRRKALFEEALALAKRGCHVDITAFPVEEGEDAWPADEALVRYLDSG
                     APAERVSISSDAGGCLPCFDAHGRVCGMDVGHSGALLETLNALLARGLPLERVLPAFT
                     SNVADLLRLPGKGRIRAGMDADLLALDANGAAGHVWLGGIAHLRAGEVVRRGMFER"
     gene            7149..7991
                     /locus_tag="PK818_02565"
     CDS             7149..7991
                     /locus_tag="PK818_02565"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HQY81651.1"
                     /translation="MCPSKVPEGEQRGWIIPIGGAEDKESRRRILKRFVQLCGGRDAR
                     IAVIPTASRLADTGKRYEDLFERLEAGSATSIDFQTREDGERDDYLRQLEGANGVFFT
                     GGNQLRISTIIGGTSAAKLIRTGNARGVHVAGTSAGASILSEHMIAFGKEGSSPRAGS
                     VRLAPGLGLTNRFIIDQHFRQRDRLGRLTAALAYNPFAIGIGLDEDTAAFIGPDNTLE
                     VEGSGAITVVDAGGMTFSSMADVRQTEGVCLLGLTVHILTAGATFNLHTRQASPGALS
                     TEKS"
     gene            8046..10826
                     /gene="cphA"
                     /locus_tag="PK818_02570"
     CDS             8046..10826
                     /gene="cphA"
                     /locus_tag="PK818_02570"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HQY81652.1"
                     /translation="MRILNRNVYVGPSHYAKFPVIRLELDLGALEQWPTGKLGNGFID
                     GLLVALPGLSEHGCSYREPGGFIRRMREGEGTWLGHVLEHVAIELQNVAGEDVTFGKT
                     RSISDDRPGVYSVVYEYAQREEGIAAGELALKLLDSLLPADLRTATDDSWNWEEERDG
                     FIRYAQRRALGPSTMSLVRAAEERGIPWLRLNQQSLVQLGHGKYQQRIQATVTGRTPH
                     IAVELASDKEETNKILGSLGLPVPRQELVTSQTDALKAARRLGGPVVLKPYNGNHGRG
                     ITINVTGEDEIRAGFEAAREHSRSVIVETYLAGDDHRLLVVNGELIAATRRTPGHVVG
                     DGRRTVAELVEVVNSDPRRGVGHEKVLTKLKLDREAELMLERKGYTADSVPPAGETVP
                     LRSTANLSTGGTATDVTDIIHPDNRAMAERAVRAVGLDVGGVDFLSTNIAESYKSIGG
                     GICEVNAAPGFRMHIAPSEGTPRDAAGPVIDMLFPPGTPSRVPIAAVTGTNGKTTTAR
                     MLAHIAKMAGYTPGLTTTDGVYIDGARTVEGDMTGPVSARMVLADPQIDLAVLETARG
                     GLVRSGMGVGEVDVGAVINVASDHLGLKGIDTLEQLAEVKRIVVEVARECAVLNADDP
                     NVMKMSAYTDAKVICYVTMNPSHPLVREHIRAGGRACALEAGVNGHMITLYDKGSHIP
                     LLWTHLIPATLEGRAMHNVQNAMVAAAMAFSLGIKLDAIRHGLRTFDTTFFQAPGRMN
                     VFSEHPFKVIMDYAHNAHAVAVMADLAQRLDAGRRIVVVAGPGDRRDEDLREIANAVA
                     GKFDHYICRRDDSLRGRDGDEVPRIIATALRAAGVAEAAITAIPDEQQAVDAALRMGR
                     PGDLVLVFADALTRSWKQIIRFKPDGDMPAATARIEVPQLETTLDEQLVAAMEGVVRD
                     ERGLRFEREESD"
     gene            10837..12606
                     /locus_tag="PK818_02575"
     CDS             10837..12606
                     /locus_tag="PK818_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HQY81653.1"
                     /translation="MSEPFEDSRRLTGANLYFDGAGAALETSPGLAFDADARQRWRDN
                     IARARATLGWPAGAVVVREHASGASLAFAAPADQLYVATEVNEWALYSALGIRATDAP
                     VDDEAASPRPHVAHFDDADALRRLQALAATEAKPALRALVEAARAHGVPAHADDDLLS
                     IGEGEAAQAWALDALPAADAVPWSRLRAIPKAVVTGSNGKTTTVRLLAAMLRAHGLRA
                     GYSSTDGLFVDGERVEAGDYSGPVGARTVLRDPRVQAAVLETARGGLLRRGLVAHDAQ
                     VAVVTNISADHFGEYGIHGLDDIAAVKLVVAKSLAADGVLVLNADDPLLVRRAVATGV
                     ARIGWFALALDDALARGARACGARAGSMLLCIDGIEHDLGAIAGMPLTLGGSARYNIA
                     NIAAASLAAHALGIAPAKIASVLARFGGCNADNPGRLQRWTLGDVQVLLDYAHNPDGL
                     RGLLQVAAGLRGNGRLGLLLGHAGNRLEADFRALAAVAAEAKPDRVWLKDIGGDYLRG
                     RASGEVAAILFGALRAAGLPAGALPVCLDEAQAACEALQWARPGDLLVLPVHEPARRD
                     VVVDLLDRLQAAGWRAGQGLADA"
     gene            12665..13456
                     /gene="trpC"
                     /locus_tag="PK818_02580"
     CDS             12665..13456
                     /gene="trpC"
                     /locus_tag="PK818_02580"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HQY81654.1"
                     /translation="MHDVLKRILVRKQEEVAERRGQVSLFELKTRAASASPPRGFADA
                     IVATIARGDAAVIAEVKKASPSKGVIRADFDPAAIARSYQAGGAACLSVLTDVDFFQG
                     ADAYLLAARAACSLPVLRKDFVVDAYQLYEARVLGADCVLLIAAALDDAQLVEYAFIA
                     GELGMDVLVEVHDLDELERALPVPARLLGINNRNLRSFEVSLQATLGLKDAVPADRVL
                     VTESGILARADVALMRAAGVHAFLVGEAFMRQPDPGTALRGLFAE"
     gene            13453..14187
                     /locus_tag="PK818_02585"
     CDS             13453..14187
                     /locus_tag="PK818_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HQY81655.1"
                     /translation="MSGQHFPEPRLDAPLVVFDFDHTLYDGDSGSHLFKWLIDRAWWR
                     QLLALLAAPVAGPMVAWLRTRRAGISVFVWIGTVGLHRRRDLDALVDRYVEAHAGAIR
                     SRLLPVALQVLHRHREAGDRVVVATGAPPELARAILAFVAHEDVPVVGTLVGPKFGAV
                     GALRHCHHAMKMTMLREAGHTQPVAIAYSDSSADLPLLQAARRPVVVNPKAARVAMFR
                     RVLPPGTPILNWGCPQRAGDPVVPQS"
     gene            complement(14194..14661)
                     /locus_tag="PK818_02590"
     CDS             complement(14194..14661)
                     /locus_tag="PK818_02590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY81656.1"
                     /translation="MSRAFSTGHHPLGTDLRMAVVADADDVARLLSDLGYPCEIDEAA
                     ERIAVIADNDRQALVVARRDGAVVGLIALDYMYYLPLGTTTCRVTALVVTPTSQGLGI
                     GRALLREAERRARVGGAARIELTSGAQRTEAHAFYRACGYKDSSVRFVKLLGA"
     gene            complement(14735..15415)
                     /gene="crp"
                     /locus_tag="PK818_02595"
     CDS             complement(14735..15415)
                     /gene="crp"
                     /locus_tag="PK818_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HQY81657.1"
                     /translation="MNAIFAKPILPRIANPLGADPATIERFIAHCHRRRYPARTDVFR
                     PGDEAGTLYYIISGSVSIIAGEEDGRELVLGYIGPGEFVGELGLFFETEQRGVALRTR
                     TACELAEIGYDRLQLLLTTQANDAARLLYSIGAQISQRLMHTSRKAGRLAFLDVSDRI
                     YRTLFDLAKEPEAMSHPQGTQLRVSRQELARLVGCSREMAGRVLKKLQIDGKLHARGK
                     TVVVYGTR"
     gene            15542..16333
                     /gene="speD"
                     /locus_tag="PK818_02600"
     CDS             15542..16333
                     /gene="speD"
                     /locus_tag="PK818_02600"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HQY81658.1"
                     /translation="MVKPLPRLKLQGFNNLTKSLSFNIYDVCYAASEDERRRYIEYID
                     EEYNADRLTQILTDVAEIIGANILNIARQDYDPQGASVTILISEQPVVDKADAKGVIS
                     DAVVAHLDKSHITVHTYPETHPDNGIATFRADIDVSTCGVISPLKALNYLIESLESDI
                     VVMDYRVRGFTRDVKGRKHYIDHKINSIQDYLAKNVRSRYEMIDVNVYQENIFHTKMH
                     MKEFDLDTYLFEEKARNLSFKERMKIEARLKREIEELYHGRNLVD"
     gene            complement(16393..16926)
                     /locus_tag="PK818_02605"
     CDS             complement(16393..16926)
                     /locus_tag="PK818_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81659.1"
                     /translation="MASHEYDPDDNFGHAFHAQSGIDDDDALEAVAWQFLLLVNPDDE
                     DAALQQFAAFQQALAEGGDGGYPVPLLRDAIDWKAGFHVAEDDAQGLMEALDELAARW
                     RLRIDWGLDDDAREAPDTTELLQAAFAQLREHHLSLWTLDTGEATLAGWITRERDEEA
                     MHLVAGALGMPARPGVG"
     gene            17028..17588
                     /locus_tag="PK818_02610"
     CDS             17028..17588
                     /locus_tag="PK818_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HQY81660.1"
                     /translation="MAAKATILKAELQVTDLDRHYYASHALTLAQHPSETDTRLLVRL
                     LAFALHADERLEFGRGLSDEDEPALWRRDYTGAIEQWIELGQPDEARLRKASGRAQQV
                     VVVSYGGQAAEAWWKRNAAALTRLRNLSVIELDDAGVAEASALLERGMRVTAMIQDGE
                     LQLMDAERSVSLRPRMRMLEGVATPD"
     gene            complement(17609..18253)
                     /gene="coq7"
                     /locus_tag="PK818_02615"
     CDS             complement(17609..18253)
                     /gene="coq7"
                     /locus_tag="PK818_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014648661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HQY81661.1"
                     /translation="MSKPRTLSPLDRLLDSAQNAVSTVAGDPRAERANPGDATPDVVL
                     DATERRHAAGLMRINHVGEVCAQALYIGQAAVARDPATRQQLLAAAQEETDHLAWCAD
                     RLRELDSRPSLLNPLWYAGSYAIGALAGLRGDGWNLGFVVETERQVEAHLEEHLQDLP
                     EADARSRAILRTMKADEARHADDAEAAGARPLPPPIPRLMAAASKLMKSVAYRL"
     gene            18432..18860
                     /gene="rplM"
                     /locus_tag="PK818_02620"
     CDS             18432..18860
                     /gene="rplM"
                     /locus_tag="PK818_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HQY81662.1"
                     /translation="MKTFIAKNETVQRDWYVVDAEGKTLGRLAAALAYRLRGKHKPVF
                     TPHVDTGDYLVVINAEKIAVTGKKLPDKMYHRFTGYVGNLKTESLAQALERHPERVIE
                     IAVKGMLPKNTLGRAMYRKLKVYKGSEHPHAAQQPQTLDI"
     gene            18863..19255
                     /gene="rpsI"
                     /locus_tag="PK818_02625"
     CDS             18863..19255
                     /gene="rpsI"
                     /locus_tag="PK818_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HQY81663.1"
                     /translation="MAISQNYGTGRRKSSTARVFLRKGTGTITVNDRPLDEFFGRETA
                     RMIVRQPLELTKNTETFDIKVTTSGGGITGQAGAIRLGIARALVEYDETLKSDLRKAG
                     FMTRDAREVERKKVGLHKARRATQFSKR"
     gene            19304..19378
                     /locus_tag="PK818_02630"
     tRNA            19304..19378
                     /locus_tag="PK818_02630"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19336..19338,aa:Gln,seq:ctg)
     gene            19501..20244
                     /locus_tag="PK818_02635"
     CDS             19501..20244
                     /locus_tag="PK818_02635"
                     /inference="COORDINATES: protein motif:HMM:NF021527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HQY81664.1"
                     /translation="MVACFAAPQASVDGLEAVLRDRGFAVLAPAGVAALAGVALAELE
                     LLAASWDDLRPDAYLKDGGRYRKRRHSCFVADAAGLRQVPHRMHWQSLDYNALHGGMQ
                     RWFDPVDPAVVARPAWSKLLLALARVCSACRPEPRWSIEAHQFRIDTADGVGRPTPEG
                     AHRDGVDFVAVVLVARNGIKGGETRVFQADGPDGQRFTLAEPWSLLLLDDAKVIHEST
                     PIQPLDGYGHRDTLVLTYRAAGFQGDDAG"
     gene            20311..>20770
                     /locus_tag="PK818_02640"
     CDS             20311..>20770
                     /locus_tag="PK818_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009594189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAA hydrolase family protein"
                     /protein_id="HQY81665.1"
                     /translation="MKLGSLKEGGRDGTLVVVSRDLTRAVRATGIAATLQQALEDWSN
                     TAPRLNALSEALDDGSADAAFDLDMQALAAPLPRAYEFVDGSAYLPHVARVRRARGAE
                     VPDSFYTDPLMYQAVSAGFLGPRDPVRVVSEDYGIDLEAEIVIVTDDVPMA"
BASE COUNT         3200 a         7141 c         7351 g         3078 t
ORIGIN      
        1 tgaactgcgc gatccaggcg agcacgaaca cgcccagcgc cagcctggcc aggccgggcg
       61 tgccgtagct gtcgtgcagc cagcgggtca gccacgccat cgccacgaag gtcgccagca
      121 tgccgaagcc gatcgcgcgc gaggcccggt aatagaacat ccatgcggcg aacatcgcca
      181 gcgccgccca caggccgggc cggaaccagc tgccgacgac cgggatgcac cagagcaggc
      241 cgaccacgct ccacaggatc agcggcaccg cgaccacgtg gatgcgctgg ttgaccgggt
      301 tccggtggtc gccggagtaa tgggcgaacc agcggtcgat ggggcgttcg gtactggcgg
      361 cagtcacggc gcgcgctcgc aggcagggtg ggccgagctt gcgccgcagg tgcccgccgg
      421 gcaaggccgg cgcgcctgcc acgcaggcat cagtcgatcg aaatcccggc cagcttctgc
      481 agcgcctcgg cgtacttggc gcgggtcctg tcgatcacgt ccgcgggcag gcgcgggccg
      541 ggtgcggtct tgttccagtc cagtgtttcc aggtagtcgc gcacgaactg cttgtcgtag
      601 ctgggcgggc tggtgccaac ctggtactgc tccgccggcc agtagcggct ggagtccggg
      661 gtgaacatct cgtccatcac gtacagcctg ccgtcgacat cggtgccgaa ctcgaacttg
      721 gtgtcggcca gcaggatccc gcgctgcgcc gcgtagtcgc gggcgaaggc gtacagcgcc
      781 agcgtcgcat cgcgcacctg ttcggcgcgt tcgcggccga ccaggtcgac cgcggtggcg
      841 aaatcgatgt tctcgtcgtg gtcgcccacc gccgccttgg tcgacggggt gaagatcggc
      901 gccggcaact gctgcgcctg ctgcaggcct gccggcagcc ggatgccgct gacttcgccg
      961 gtgcgctggt agtccttcca gccgctgccg atgatgtagc cgcgggcgat gcattccacc
     1021 ggtaccgggg tcagcttctt cgtgaccacc gcacgttttg cgtacagcgc gggatccacg
     1081 ccggccggca gcaccgaggc gacatcgatg ccggtcaggt ggttggcgat gatgtgcgcg
     1141 gtcttgtcga accagaaatt gctgatctgg cagagcatct cgcccttgcc ggggatcggg
     1201 tcgggcagca ccacgtcgaa cgcgctcagg cggtcggtcg cgaccatcag caggtggccg
     1261 tcgccgaggt cgaacacgtc gcgcaccttg ccgcggtgga tcaggttcag gccggggagg
     1321 tcggactgga gcagggtggt ggccatgcgc gcgcgtgcaa tcgaggggcg cctagtctaa
     1381 ggccggcgga tgccccgcgc cctgttgccg gccttgtaag atggcgccca tgaaacgctg
     1441 gtacggcaag ttcttcgggc tgatcgcggg ttggctgctg ctgcggcatc cggcgggcgg
     1501 cctgctgggc ctgctgatcg gccatgcgtt cgatgccgac tggttcaagt ccacccgcga
     1561 caacccctac cgggtgttcg gcctgaccga ggaagcctcc gacgccgaga tcgacctggc
     1621 ctatcgccgc ctgatctcgc aataccaccc ggaccgcgtg gccggtgccg cgccggaact
     1681 gcagcgccag gccgaagcca aggcgcgcga gatcaacgcg gcctacgacc gcatcaaggc
     1741 gctgcggaac cggaagtgat gtcctccccg ccttcgcggg gagcccggga cgttagactt
     1801 ggcagcccaa agccaatgga ttcccgcctt cgcgggaatg acgagcatca tgcaaccgac
     1861 cgtcatcgca ccttcgatcc tttccgccaa cttcgccagg ctcggcgaag aggtggacaa
     1921 cgtgctggcg gccggtgccg actgggtgca tttcgacgtg atggacaacc attacgtgcc
     1981 caacctcacc atcggcccgc tggtgtgcga ggcgttgcgc aagcatggcg tcaccgcccc
     2041 gatcgacgtg cacctgatgg tggagccggt ggacggcctg atcccgatgt tcgccgacgc
     2101 cggcgcatcg cacatcagct tccatcccga ggccagcagg cacgtccatc gcagcgtgca
     2161 gctgatcaag tcgaagggct gccaggccgg gctggtgttc aacccggcca gttctctgga
     2221 cgtgctggag gtgatgctgg acgagatcga ctacgtgctg ctgatgtcgg tcaatcccgg
     2281 cttcggcggg cagtcgttca tcgcgtccgc gctccgccag ctgcgccgcg tgcgcgagct
     2341 gatcgacgcc agcggccggc cggtccggct ggagatcgac ggcggggtga agccggacaa
     2401 catcgcggac atcgccgcag cgggcgcgga taccttcgtc gccggcagcg cgatcttcgg
     2461 caagcccgac tacgccgcgg tcatcgcccg gatgaagcag gcggtggcgg cggtgcgctg
     2521 agcacgggcg gcatcgctgg aaaaagaaag ccgacgccgc gggaggggtg cggcaccggc
     2581 ttcggggaag ggtgggcgtc ctgcccgtca atcgtccctg caatggcctt cgatgatccg
     2641 cgcggcgcgt gacgccggcg tgacagccgg ggcgggtttg ccgaagcccg cccgcggcgg
     2701 ctacagttgg cgcatgaacc gggcgattcc gtctttcgaa cagggttggc gatggtggcg
     2761 agattccgcc gcccaggtcg ctcccatgcc catgaaggac accccgcttg atcacgcacg
     2821 cccagttcga ccagcacgcc gctgacggcc ataccctgat cccggtcgtg cgcgaggtcc
     2881 tgtccgacct cgacaccccg ctgtcggtct acctgaagct ggccgacggc ccgcacacct
     2941 acctgttcga atcggtggag ggcggcgagc gcttcggccg ctattcgatc atcggcctgc
     3001 cggcgcggcg ggtgtacgag gtgcgcggcc acgcgctgca ggtgcgcgag cacggcgaag
     3061 tcgtggagac ccgcgaactc gacgatccgc tggccgaggt cgaacgcctg cgcgcgcact
     3121 accgggtgcc gaagctggac ggcctgcccg gcttttcagg cgggctggtg ggctggttcg
     3181 gcttcgagtg catcgaatac atcgagccat gcctgcgcgc gaacccgctg ccggacgaac
     3241 tcggcacgcc cgacatcctg ctgatgctca gcgaggaact ggcggtgttc gacaacctca
     3301 agggccggct gtacctgatc gtgcacgccg acccgcgcca gccgcaggcg tgggcgcgcg
     3361 cgcagcggcg gctggatgcg ctggcgcacc gcctgcgcca ggccgggcgc ggctatccgg
     3421 aaaccctgca cggcaacgcc ctcgacgagg ccgatttcgt cagcggcttc acccgcgagg
     3481 gcttcatcga ggcggtccat cgcagcaagc agtacatcgc atccggcgat gccttccagg
     3541 tggtgctcag ccagcgcctg agcgtgccgt tccgcgcgcg cccggtggac gtgtaccgcg
     3601 cgctgcgggc gacgaatccg tcgccgtaca tgtatttcct ggataccggc gcgacccagg
     3661 tggtcggttc ctcgccggag atcctggtcc gcctgcaaca taccgttgac ggcgggggcg
     3721 aagtcaccgt gcgcccgatc gccggcaccc gcccgcgcgg ccggacccac gaggaagacc
     3781 tggcgctgga agccgagttg ctggccgacc cgaaggagcg cgccgagcac ctgatgctga
     3841 tcgaccttgg ccgcaacgat gccggccgcg tgtccgagcc gggcacggtg gtggtgggcg
     3901 agcagttcgt gatcgaacgc tacagccacg tcatgcacat cgtcagcgaa gtcaccggtc
     3961 gcctgaagca gggcctgtcc tacatggacg tgctgcgcgc caccttcccc gccggcaccg
     4021 tcagcggcgc gccgaagatc cgcgcgctgc agatcatccg cgagctggaa ccggtcaagc
     4081 gcaacatcta ctccggcgcg gtcggctaca tcggctggca cggcgacgcc gacaccgcca
     4141 tcgccatccg caccgcggtg atccaggacg gccgcctgca cgtgcaggcc ggtgccggca
     4201 tcgtctacga ctccgacccg caggccgagt gggacgagac catgaacaag ggccgcgcgc
     4261 tgttccgcgc ggtggccgaa gcggcgaagg ggttgtgacg tgctgctgat gatcgacaac
     4321 tacgactcct tcacctggaa cctcgtccag tacctgcagt cgctgggcgc ggaggtgcgg
     4381 gtggagcgca acgatgcgct gacggtggac gagatcgagc ggctggcgcc ggaacgcatc
     4441 gtgatctcgc ccgggccgtg cacgccgaac gaggcagggg tgtcgatggc ggtggtcgag
     4501 cgcctgggcg cgaccacgcc gatcctcggc gtgtgcctgg gccaccagag catcggccag
     4561 gtctacggcg gcgacgtggt gcgcgcgggc aagatcatgc atggcaagac ctcgcgcatc
     4621 cgccaccagg gcaagggcgt gttcgccggc ctgccggacg gctttgaggc cacccgctac
     4681 cactcgctgg tggtggacaa ggccacgctc ccggatgcgc tggaaatcac cgcgtggacc
     4741 gagaacgacg acggttcgat ggaggcgatc atgggcctgc gccatcgcgg gcatccggtc
     4801 gaaggcgtgc agttccatcc cgaatccatc ctgacccagc acggccacgc gctgctgaag
     4861 aacttcctgg agcgctgacc cgatgccctt caccccgcag gacgcgctgc agcgcgccat
     4921 cgagcaccgc gagatcttct tcgacgaaat ggtcgagctg atgcgccagg tcatgcgcgg
     4981 cgaggtttcg ccggtcatga ccgcggcgat cctcaccggg ctgcgggtga agaaggaaac
     5041 cgtcggcgag atcgccgccg cggcgaccgt gctgcgcgaa ttcgcgcgtc cggtcgacgt
     5101 cgccgaccgc acgcacctgg tcgacatcgt cggcaccggc ggcgatggcg cgcacacctt
     5161 caacatctcc accgcgagca tgttcgtggt ggcggcggcc ggtggcaagg tcgccaagca
     5221 cggcaaccgc agcgtgtcgt cgaagtcggg cagcgccgac gtgctggaag cgctgggcgc
     5281 caacatcgag ctgcagccgg ggcaggtcgc ggcctgcatc gagcgctgcg ggatcggctt
     5341 catgttcgcc cccgtccacc acccggcgat gaaggtggtg gcgccggtgc ggcgcgagat
     5401 gggcgtgcgc acgctgttca acatccttgg cccgctgacc aatccggcca atgcccccgg
     5461 catcctgatg ggcgtgttcc atccggacct ggtcggcatc caggtgcgcg tgctgcagga
     5521 actcggcgcg cagcgcgcgc tggtggtgtg gggccgcgac ggcatggacg agctctcgct
     5581 gggcgccggc accctggtcg gcgagctgcg cgatggcgtg gtccgcgaat acgaactgca
     5641 tcccgaggac ttcggcatcg cgatggcgca cagccgcaac ctgcgggtcg ggaacgccac
     5701 cgaatcgatg gacctgctgc gccaggcgct ggacaaccgc gagtgcctgc cgcgcgagat
     5761 cgtcgcctac aacgcaggcg cggcgctgta cgcggcgggc gtggccagcg acatcggcga
     5821 cggcatcctg cgcgcgcgca atgcgatcgc gtccggcgcg gcggccgcga agctggacga
     5881 attcgtcgcc accacgcagg cgctggcggg cgcttgacgc tgccatccac gcgccgcacc
     5941 ctgccgcgat gaccgcgccc ctgctgctgc tgcgcaatgc cgacgtgtac gcgccggaac
     6001 cgctgggccg tcgccacctg ttgctgggtg gcggcaaggt gttgtggatg ggcgggtccg
     6061 ggcccggatt gccggaagcg ctcggcgtcg aaaccatcga tctcgaaggc gcgcgcctgc
     6121 tccccggctt catcgacggc cacgtccacg ccagcggtgg tggcggcgaa gccggcttcg
     6181 ccagccgggt gccggcgccg ctgctgtcgc gctacaccag cgccggggtg accacggtca
     6241 ttggcctgct cggcaccgac gatgtctcgc gcggcaccag cgagctgctt tcgcatgtgt
     6301 acgcgctgcg cgagcagggc ctcggcgcgt gggcgtattg cggcggctac cacatcccgc
     6361 cgaccacgct gaccggcagc gtgcgcggcg acatcgtgtt cagcgatgcg ctgatcggcg
     6421 tgggcgaagt ggcgatcagc gaccaccgtt ccagccaacc aacgctggac gacgtgctgc
     6481 ggctggcatc cgaggcgcac gtggccgggc tgatgaccgg caaggccggc atcgtccacc
     6541 tgcacctcgg cgatggcccg cgcgggctgg aactggtgcg gcaggcgctg gacaccagcg
     6601 aactgccgcc gcgggtgttc cagccgacgc atgtgaaccg caggaaggcc ttgttcgagg
     6661 aagcgctcgc actggcgaag cgtggctgcc atgtcgacat caccgcgttc ccggtcgagg
     6721 agggcgaaga cgcctggccc gccgacgagg cgctggtgcg ctacctggac agcggcgcgc
     6781 cggcggagcg ggtcagcatc agctccgacg ccggcggctg cctgccctgc ttcgatgcgc
     6841 atggccgtgt ctgcggcatg gacgtgggcc actccggcgc cctgctggag accctgaatg
     6901 ctttgctggc ccgcggcctg ccgctggaac gcgtgctgcc cgccttcaca agcaacgtgg
     6961 cggacctgct gcggctgccg ggcaagggcc ggatccgggc aggcatggat gccgacctgc
     7021 tggcgctgga cgcgaacggt gcggccggtc acgtctggct gggcgggatc gcccacctgc
     7081 gggcgggcga ggtggtgcgc cgtggtatgt tcgagcgctg atcgcagcaa gcccaggaag
     7141 cgtcgcgcat gtgtccaagc aaggtccccg agggtgagca gcggggctgg atcattccca
     7201 tcggcggcgc cgaggacaag gaaagccggc ggcggatcct caagcggttc gtgcagctgt
     7261 gcggtggccg cgacgcgcgg atcgcggtga tcccgaccgc cagccggctg gccgataccg
     7321 gcaagcgcta cgaggacctg ttcgagcgcc tggaggcggg cagcgcgacc tcgatcgact
     7381 tccagacccg cgaggacggc gagcgcgacg actacctgcg ccagctggaa ggcgccaacg
     7441 gcgtgttctt caccggcggc aaccagctgc gcatctccac catcatcggc ggcaccagcg
     7501 cggccaagct gatccgcacc ggcaacgcgc gcggggtgca cgtggccggc accagcgccg
     7561 gcgccagcat cctgtccgag cacatgatcg cgttcggcaa ggaaggctcc tcgccgcgcg
     7621 ccggcagcgt gcggctggcg cccggactgg gcctgaccaa ccgcttcatc atcgaccagc
     7681 atttccgcca gcgcgaccgc cttggccgcc tgaccgcggc gctggcctac aacccgttcg
     7741 cgatcggcat cggcctggac gaggacaccg ccgccttcat cggcccggac aacaccctgg
     7801 aagtggaagg cagcggcgcg atcacggtgg tcgatgccgg cggcatgacc ttctcgtcga
     7861 tggccgacgt gcgccagacc gaaggcgtgt gcctgctcgg gctgaccgtg cacatcctga
     7921 ccgccggtgc caccttcaac ctgcacacgc gccaggcctc gccgggcgcg ctgtcgaccg
     7981 aaaagagctg attcccaggc aaaccacgcg aaacacccag gttcggattt gagcgggagg
     8041 gctcgatgcg catcctcaat cgcaatgtct atgtcgggcc ctcgcactac gcgaagttcc
     8101 cggtgatccg gctcgagctc gacctcggcg cgctcgagca gtggcccacc ggcaagctcg
     8161 gcaacggttt catcgatggc ctgctggtgg cgctgccggg cctgagcgag cacggctgct
     8221 cgtaccgcga gccgggcggc ttcatccgcc gcatgcgcga gggcgagggc acctggctcg
     8281 ggcatgtgct ggagcatgtc gcgatcgaac tgcagaacgt ggccggcgag gacgtgacct
     8341 tcggcaagac ccgcagcatt tccgacgacc gccccggcgt gtattcggtg gtgtacgagt
     8401 acgcccagcg cgaggagggc atcgctgccg gcgaactcgc cctgaagttg ctggattcgc
     8461 tgctgccggc ggatttgcgc acggccaccg acgattcctg gaactgggag gaggagcgcg
     8521 acggcttcat ccgctatgcg cagcggcgcg cgctggggcc atcgacgatg tcgctggtgc
     8581 gcgcggcgga agagcgcggg atcccgtggc tgcgcctcaa ccagcagtcg ctggtgcagc
     8641 tcggccacgg caagtaccag cagcggatcc aggccacggt caccgggcgc accccgcaca
     8701 tcgcggtgga gctggccagc gacaaggaag agaccaacaa gatcctcggc tcgctcggcc
     8761 tgccggtgcc gcgccaggag ctggtgacca gccagaccga cgcgctcaag gctgcgcgca
     8821 ggctcggcgg cccggtggtg ctcaagccat acaacggcaa ccacggccgc ggcatcacca
     8881 tcaacgtcac cggcgaggac gagatccgcg ccggcttcga ggccgcgcgc gagcattcgc
     8941 gctcggtgat cgtggaaacc tacctggccg gcgacgacca ccgcctgctg gtggtcaatg
     9001 gcgagctgat cgccgcgacc aggcgcaccc ccggccatgt ggtcggcgat ggccggcgca
     9061 cggtcgcgga gctggtggaa gtggtcaaca gcgatccgcg ccgcggcgtc ggccatgaaa
     9121 aggtgctgac caagctcaag ctcgaccgcg aggccgagct gatgctggaa cgcaagggct
     9181 acaccgcgga ctcggtgccg ccggccgggg aaaccgtgcc gctgcgttca accgccaacc
     9241 tgtccaccgg cggcaccgcc accgacgtca ccgacatcat ccatccggac aaccgggcga
     9301 tggccgaacg cgcggtgcgc gcggtcgggc tggacgtggg cggcgtcgat ttcctgtcca
     9361 ccaacatcgc cgagagctac aagtcgatcg gcggcggcat ctgcgaggtc aatgccgcgc
     9421 ccggcttccg catgcacatc gcgcccagcg aaggcacccc gcgcgatgcc gccggcccgg
     9481 tgatcgacat gctgttcccg ccgggcacgc cgtcgcgggt gccgatcgcc gcggtgaccg
     9541 gcaccaacgg caagaccacc accgcgcgca tgctggcgca catcgccaag atggccggct
     9601 acacgccggg cctgaccacc accgacggcg tctacatcga cggtgcgcga acggttgaag
     9661 gcgacatgac cggcccggtg tccgcgcgga tggtgctggc cgacccgcag atcgacctgg
     9721 ccgtgctgga gaccgcgcgt ggcggcctgg tgcgctcggg catgggcgtg ggtgaggtgg
     9781 acgtcggcgc ggtgatcaac gtcgcctccg accacctcgg gctgaagggc atcgataccc
     9841 tcgaacagct ggccgaggtc aagcgcatcg tggtggaagt cgcgcgcgag tgcgcggtgc
     9901 tcaacgccga tgatccgaac gtgatgaaga tgtccgcgta caccgacgcg aaggtcatct
     9961 gctacgtgac catgaacccc tcgcatccgc tggtgcgcga acacatccgc gccggcggcc
    10021 gcgcctgcgc gctggaagcc ggcgtcaacg gccacatgat caccctgtac gacaagggca
    10081 gccacatccc gctgctgtgg acgcacctga tcccggcgac cctggaaggc cgcgcgatgc
    10141 acaacgtgca gaacgcgatg gtcgccgcgg cgatggcgtt ctcgctgggg atcaagctgg
    10201 acgcgatccg ccacggcctg cgcaccttcg acaccacctt cttccaggcc ccggggcgga
    10261 tgaacgtgtt cagcgagcac ccgttcaagg tgatcatgga ctacgcccac aacgcccacg
    10321 cggtggcggt gatggcggac cttgcgcagc ggctggatgc cggccgcagg atcgtggtgg
    10381 tggccggccc cggcgaccgc cgcgacgagg acctgcgcga gatcgccaat gccgtcgccg
    10441 gcaagttcga ccactacatc tgccgccgcg acgattccct gcgcggccgc gacggcgacg
    10501 aggtgccgcg gatcatcgcc accgcgctgc gggccgccgg cgtcgccgag gccgcgatca
    10561 ccgccatccc cgacgaacag caggcggtcg acgccgcgct gcgcatgggc cggcccggcg
    10621 acctggtgct ggtgttcgcg gacgcgctga cccggtcgtg gaagcagatc atccgcttca
    10681 agccggatgg cgacatgccc gccgccaccg cgcgcatcga ggtgccgcag ctggagacca
    10741 ccctggacga gcaactggtc gcggcgatgg aaggcgtggt gcgcgacgag cgcgggctgc
    10801 gcttcgagcg cgaagagtcc gattgagccg cgcatcgtga gcgaaccgtt cgaggattcg
    10861 cgccggctga ccggcgccaa cctctacttc gatggcgccg gcgcggcgct ggaaacctcg
    10921 cccggactgg cattcgatgc cgacgcccgg caacgctggc gcgacaacat cgcgcgggcg
    10981 cgggcaacgc tcggttggcc cgcgggcgcg gtcgtcgtgc gcgaacatgc cagcggcgca
    11041 tcgcttgcct tcgcggcgcc ggccgaccag ctctacgtcg ccaccgaagt gaacgagtgg
    11101 gcgctgtatt cggcgctcgg tatccgcgca accgatgcgc cggtggacga cgaagccgcg
    11161 tcgccgcgcc cgcatgtcgc ccatttcgat gacgccgacg ccctgcgccg gttgcaggcg
    11221 ctcgccgcga cggaagcgaa gccggcgctg cgcgcgctgg tcgaggccgc gcgtgcgcat
    11281 ggcgtccccg cgcatgcgga cgacgacctg ctctcgatcg gcgagggcga agccgcgcag
    11341 gcatgggcgc tggatgcctt gccggctgcg gatgcggtgc cgtggtcgcg cctgcgcgcg
    11401 atcccgaaag ccgttgtcac cggttccaac ggcaagacca ccacggtccg cctgctggcg
    11461 gcgatgctgc gtgcgcatgg cctgcgcgcc ggctacagca gcaccgacgg cctgttcgtc
    11521 gatggcgaac gggtcgaggc cggcgactat tccggcccgg tcggcgcgcg caccgtgttg
    11581 cgcgacccgc gggtgcaggc ggcggtgctg gaaaccgcgc gcggcggcct gctccgacgc
    11641 ggcctggtgg cgcacgatgc gcaggtggcg gtggtcacca acatcagcgc cgaccacttc
    11701 ggcgagtacg gcatccacgg cctcgacgac atcgccgcgg tcaagctggt ggtcgcgaag
    11761 tcgctggcgg cggatggggt gctggtgctc aatgccgatg acccgttgct ggtgcggcgc
    11821 gcggtggcga ccggcgtggc ccgcatcggc tggttcgcgc tggcgctgga cgatgcgctg
    11881 gcgcgcggcg cgcgcgcatg cggggcgcgt gctggcagca tgctgctctg catcgatggc
    11941 atcgaacacg acctcggcgc gatcgcaggc atgccgctca ccctcggcgg cagcgcgcgc
    12001 tacaacatcg ccaacatcgc ggccgccagc ctcgccgcgc atgcgctggg catcgcgccg
    12061 gcgaagatcg cctcggtact ggcgcgcttc ggcggctgca acgcggacaa ccccggccgc
    12121 ctgcagcgct ggaccctggg cgacgtgcag gtgctgctgg attacgcgca caacccggac
    12181 gggctgcgcg ggctgctgca ggtcgccgcg ggcttgcgcg ggaacgggcg cctgggcttg
    12241 ctgctgggac acgccggcaa ccggctggag gcggatttcc gcgcgctggc ggcggttgcc
    12301 gccgaggcga agcccgaccg ggtctggttg aaggacatcg gcggcgacta cctgcgcggg
    12361 cgtgcgtcgg gcgaagtcgc ggcgatcctg ttcggcgcct tgcgcgcggc cggcctgccc
    12421 gcaggtgcct tgccggtctg cctggacgag gcgcaggccg cgtgcgaagc cctgcagtgg
    12481 gcgcgacccg gtgacctgct ggtgctgccg gtccacgaac ccgcgcggcg cgacgtggtg
    12541 gtggacctgc tagaccgctt gcaggccgcg ggctggcggg cggggcaagg gctcgcggac
    12601 gcctgagtgc acgcgaccgc ggcgggctcg attaaaatcg tgctcccccc gagcggcgaa
    12661 cgacatgcat gacgtgctca agcgcatcct ggtgcgcaag caggaagagg tggccgagcg
    12721 ccgcgggcag gtttcgctgt tcgagctgaa gacccgcgct gcatcggcgt cgccgccgcg
    12781 cgggttcgcc gatgcgatcg ttgcaaccat cgcgcgcggc gatgccgcgg tgatcgcgga
    12841 agtgaagaag gccagcccgt cgaagggcgt gatccgcgcc gacttcgatc cggccgcgat
    12901 cgcccgcagt taccaggctg gcggcgcggc ctgcctgtcg gtgctgaccg acgtcgactt
    12961 cttccagggc gccgatgcct acctgctggc cgcgcgcgcc gcgtgttcgc tgccggtact
    13021 gcgcaaggac ttcgtcgtcg atgcgtacca gctgtacgaa gcgcgcgtgc tgggcgccga
    13081 ctgcgtgctg ctgatcgccg cggcgctgga cgacgcgcaa ctggtcgagt acgcgttcat
    13141 cgccggcgag ctggggatgg acgtgctggt ggaagtgcac gacctcgacg aactggagcg
    13201 cgcgctgccg gtgccggcgc gcctgctggg gatcaacaac cgcaacctgc gcagcttcga
    13261 ggtgtcgctg caggccaccc tcggcctcaa ggacgcggtg cccgcggatc gggtgctggt
    13321 gaccgagagc gggatcctcg cgcgggccga cgtggccctg atgcgcgccg ccggggtgca
    13381 tgcgttcctg gtcggcgagg cgttcatgcg ccagcccgac ccgggcaccg cgctgcgcgg
    13441 gctgttcgcc gaatgagtgg acagcacttc cccgagccgc gcctggacgc gccgctggtg
    13501 gtgttcgact tcgaccacac cctctacgac ggcgattccg gcagccacct gttcaagtgg
    13561 ctgatcgatc gcgcctggtg gcgccagttg ctggcgttgc tggccgcgcc ggtcgccggg
    13621 ccgatggtgg cgtggctgcg cacgcgccgc gccgggatct cggtgttcgt gtggatcggg
    13681 accgtcggcc tgcatcgccg gcgtgatctc gatgcattgg tcgaccgcta cgtggaagcg
    13741 cacgccgggg cgatcaggtc gcgcctgctg ccggtcgcgc tgcaggtgct gcaccgccat
    13801 cgcgaggccg gcgaccgcgt ggtggtcgcc accggcgcgc cgccggagct ggcgcgcgcg
    13861 atcctcgcgt tcgtcgccca cgaagacgtg ccggtggtcg gtaccctggt cgggccgaag
    13921 ttcggcgcgg tcggcgcgct gcgccactgc caccacgcga tgaagatgac catgctgcgc
    13981 gaagccggcc acacccagcc ggtggcgatc gcctacagcg acagcagcgc cgacctgccg
    14041 ctgctgcagg ccgcccggcg gccggtggtg gtgaatccga aggcggcgcg ggtggcgatg
    14101 ttccgccggg tgctgccgcc cggcacgccg atcctcaact ggggctgccc gcaacgcgcg
    14161 ggcgatccgg tggtgccgca gtcctaggcc gggtcaggcg cccagcagct tgacgaagcg
    14221 caccgagctg tccttgtagc cgcaggcgcg atagaacgca tgcgcctcgg tgcgctgcgc
    14281 gccgctggtc agctcgatcc gcgcggcacc gccgacgcgg gcgcggcgct cggcttcgcg
    14341 cagcagggcg cggccgatgc ccagcccctg cgaggtcggg gtcaccacca gcgcggtcac
    14401 ccggcaggtg gtggtgccca gcggcaggta gtacatgtag tcgagcgcga tcaggccgac
    14461 caccgcgcca tcgcggcgcg ccaccaccag tgcctggcgg tcgttgtcgg cgatcaccgc
    14521 gatgcgttcc gccgcctcgt cgatctcgca gggatagccg aggtcgctga gcaggcgggc
    14581 gacgtcgtcg gcgtcggcaa ccaccgccat gcgcaggtcg gtgccaaggg gatggtggcc
    14641 ggtcgagaac gcgcggctca ttcgctcatg tcccccgcga tggcgtcatg cggcgggtcc
    14701 gtgtgcggcg gcgcgcaggt cggccggctc cacgtcagcg cgtgccgtac acgaccacgg
    14761 tcttgccgcg cgcgtgcagc ttgccgtcga tctgcagctt cttcagcacc cgcccggcca
    14821 tctcccgcga acagccgacc agccgcgcca gctcctgccg cgagacccgc aactgggtgc
    14881 cttgcggatg gctcatcgcc tccggctcct tggcaaggtc gaacagggtc cggtagatgc
    14941 ggtcgctgac gtcgaggaac gccaggcggc ccgccttgcg gctggtatgc atcaggcgct
    15001 ggctgatctg ggcaccgatc gagtacagca ggcgcgcggc gtcgttggcc tgggtggtca
    15061 gcagcagctg caggcggtcg tagccgatct cggcgagttc gcatgcggtg cgggtgcgca
    15121 gggcgacccc gcgctgctcg gtctcgaaga acagccccag ctcgccgacg aattcgccgg
    15181 ggccgatgta gcccagcacc agttcacgcc cgtcttcctc gccggcgatg atgctgaccg
    15241 agccggagat gatgtagtaa agcgtcccgg cctcgtcgcc ggggcggaac acgtcggtgc
    15301 gcgccgggta gcggcggcgg tggcagtgcg cgatgaagcg ctcgatggtc gccggatccg
    15361 cacccaacgg gttggcgatt cgcggcagga tcggtttggc gaaaatggcg ttcatgctgg
    15421 ctcgaacggg attcgcctgc actttaggcc gaaccgcggt ggggggcaaa cggcgcgcac
    15481 gcgcgatccg gcgctttgcc gcataatcgc ggccctgttc gtgccctccg gatgccccgc
    15541 cgtggtcaaa ccgctgccgc gcctgaagct gcaaggcttc aacaacctca ccaagtcgct
    15601 gagcttcaac atctacgacg tgtgctacgc cgcgtcggag gacgagcgca ggcgctacat
    15661 cgagtacatc gacgaggaat acaacgccga ccgcctgacc cagatcctga ccgacgtggc
    15721 ggagatcatc ggcgccaaca tcctcaacat cgcgcgccag gactacgacc cgcagggcgc
    15781 gtcggtgacg atcctgatct ccgagcagcc ggtggtcgac aaggccgatg ccaagggcgt
    15841 gatctccgac gcggtggtcg cccacctcga caagtcgcac atcaccgtgc acacctatcc
    15901 ggaaacccat ccggacaacg gcatcgccac cttccgcgcc gacatcgacg tgtccacctg
    15961 cggggtgatc tcgccgctga aggcgctgaa ctacctgatc gagtcgctgg aatccgacat
    16021 cgtggtgatg gactaccgcg tgcgcggctt cacccgcgac gtgaagggcc gcaagcacta
    16081 catcgaccac aagatcaact cgatccagga ctacctggcc aagaacgtgc gctcgcgcta
    16141 cgagatgatc gacgtcaacg tgtaccagga aaacatcttc cacaccaaga tgcacatgaa
    16201 ggaattcgac ctcgacacct acctgttcga ggagaaggcc aggaacctct cgttcaagga
    16261 gcggatgaag atcgaggcgc gcctcaagcg cgagatcgag gagctctacc acggccgcaa
    16321 cctggtcgac tgacggcggc gttcgctggc ggggaaacaa gccggcgcag gccggctttt
    16381 tcgtgggcgg ggtcagccca ccccgggcct tgccggcatg cccagcgcgc cggcgaccag
    16441 gtgcatcgcc tcctcgtcgc gttcgcgggt gatccagccg gccagggtcg cctcgccggt
    16501 gtcgagcgtc cacaggctca aatggtgttc gcgcagctgg gcgaacgcgg cctgcagcaa
    16561 ctcggtggtg tccggtgcct cgcgggcgtc gtcgtccagc ccccagtcga tccgcaggcg
    16621 ccagcgcgcg gccagttcgt ccagcgcctc catcaggccc tgcgcatcat cctcggcgac
    16681 gtggaagccg gccttccagt cgatcgcatc gcgcagcagc ggcaccgggt agccgccgtc
    16741 gccgccttcg gcaagcgcct gctggaacgc cgcgaactgc tgcagggcgg cgtcctcgtc
    16801 gtccgggttg accagcagca ggaattgcca ggccaccgcc tccagtgcgt cgtcgtcgtc
    16861 gatcccggat tgcgcgtgga aggcgtgccc gaagttgtcg tccggatcgt attcgtggct
    16921 ggccatcggg tgcctccagt gcaggcgcgc agcatacgtc ggcggctgcg gcggcgtgtc
    16981 cgtagaatgg tgcacccccg atccggcccc gtggcccagg acgcgccatg gccgccaagg
    17041 ccaccatcct caaggccgag ctgcaggtca ccgacctcga tcgccattac tacgccagcc
    17101 atgcgctgac cctggcccag catccgtcgg aaaccgacac ccgcctgctg gtgcggctgc
    17161 tggcgttcgc cttgcatgcc gacgagcgcc tggagttcgg ccgcggcctc agcgacgagg
    17221 acgagccggc gctgtggcgg cgcgactaca ccggcgccat cgagcagtgg atcgagctcg
    17281 ggcagccgga cgaggcgcgc ctgcgcaagg ccagcgggcg cgcccagcag gtggtggtcg
    17341 tcagctacgg cgggcaggcg gcggaggcat ggtggaagcg caatgcggcc gcgctcacac
    17401 gcctgcgcaa cctcagcgtg atcgaactgg acgacgccgg cgtggccgag gcaagcgcat
    17461 tgctggagcg cgggatgcgg gtcaccgcga tgatccagga cggcgagctg cagctcatgg
    17521 atgccgagcg cagcgtgagc ctgcggccgc ggatgcggat gctggagggc gtggcgaccc
    17581 cggactgagc gtcccgggcc gtcgtgcctc agagccggta ggcgaccgat ttcatcagtt
    17641 tcgatgccgc cgccatcagg cgcgggatcg gtggcggcaa cggcctggcg ccggcggcct
    17701 cggcgtcgtc ggcgtggcgg gcttcgtctg ccttcatcgt gcgcaggatc gcccggctgc
    17761 gggcgtcggc ctcgggcagg tcctgcaggt gctcttcgag gtgcgcctcg acctggcgtt
    17821 cggtctcgac cacgaagccc aggttccagc catcgccacg caggccagcg agcgcaccga
    17881 tcgcatagct gccggcgtac cacagcgggt tgagcaggct tggccggctg tccagttcgc
    17941 gcaggcggtc cgcgcaccag gccaggtggt cggtttcctc ctgcgccgcg gccagcagct
    18001 gctggcgggt ggcggggtcg cgcgcaaccg cggcctggcc gatgtacagc gcctgcgcgc
    18061 agacctcgcc gacgtggttg atccgcatca gcccggcggc gtggcggcgc tcggtggcgt
    18121 ccaggaccac gtcgggggta gcgtcgccgg ggttggcgcg ctcggcgcgg ggatcgccgg
    18181 ccacggtgga cacggcgttc tgggccgagt ccagcaggcg gtccagcggg ctcagggtgc
    18241 gcggtttcga catgcctgca ttctaggtcg ccaggcggcg gcttgcgcgg gccgggccgg
    18301 cgggctatca tcccgctcct ttcgcgccga aaacccgttt ccgggccgga acgaaggaac
    18361 aacgcgaggc gacgtttcgc agccgcaacg gcgagaaacc agcccgacca tgcacccctg
    18421 aagagttccc catgaagact ttcatcgcca agaacgagac cgtccagcgc gactggtacg
    18481 tcgtcgacgc cgagggcaag accctgggcc gcctggccgc agcactggcc taccgcctgc
    18541 gcggcaagca caagcccgtt ttcaccccgc acgtcgatac cggcgattac ctcgtcgtga
    18601 tcaacgccga gaagatcgcg gtgaccggca agaagctgcc cgacaagatg tatcaccggt
    18661 tcaccggtta cgtcggcaac ctgaagaccg aatccctggc acaggcgctg gagcgccacc
    18721 cggagcgcgt gatcgagatc gcggtcaagg gcatgctgcc gaagaacacc ctgggccgcg
    18781 cgatgtaccg caagctcaag gtctacaagg gctccgaaca cccgcacgct gctcagcagc
    18841 cgcagacgct ggacatctga ccatggcgat ttcgcagaat tacggcaccg gccgtcgcaa
    18901 gtcctccacc gcccgcgtgt tcctgcgcaa gggcaccggc accatcaccg tcaacgaccg
    18961 tccgctggac gagttcttcg gccgtgagac cgcgcgcatg atcgtgcgcc agccgctgga
    19021 gctgaccaag aacaccgaga ccttcgacat caaggtcacc accagcggtg gcgggatcac
    19081 cggccaggcc ggtgcgatcc gcctcggcat cgcccgcgcg ctggtcgagt acgacgagac
    19141 cctgaagagc gacctgcgca aggccgggtt catgacccgc gatgcgcgcg aggtcgagcg
    19201 caagaaggtc ggcctgcaca aggcccgccg cgccacccag ttctccaagc gttaagcgat
    19261 gcctgcgccg gtgttccggc gccggcgtta caatcagttc catagccccg tcgccaagcg
    19321 gtaaggcacc tgactctgac tcaggcattc ggtggttcga atccatccgg ggctgccagt
    19381 ttcaacacca cgcaagcacg accaggcccg cccgcaaggc gggcttcgtc gtttccgggg
    19441 catccgcctg cagggcgtgg cggggcggtg cgacaatgct cggcttcgcc ggaggtgccg
    19501 atggtcgcct gctttgccgc gccgcaagcg tccgtggatg gtctggaagc ggtcctgcgc
    19561 gaccgcggtt tcgcggtgct cgcgcccgcg ggcgtggccg cgctggccgg cgtggcgctg
    19621 gccgagctcg agctgctggc ggcgagctgg gatgacctgc ggcccgacgc ctacctgaag
    19681 gatggcgggc gctaccgcaa gcgccgccat tcctgcttcg tcgccgacgc cgccgggctg
    19741 cgccaggtgc cgcatcgcat gcactggcaa tcgctcgact acaacgcgct gcacggcggc
    19801 atgcagcgct ggttcgatcc ggtcgatccg gcggtggtgg cgcggccggc gtggtcgaag
    19861 ctgctgctgg cgctggcccg ggtctgctcc gcttgccggc cggagccgcg ctggtcgatc
    19921 gaagcgcacc agttccgcat cgacaccgcc gacggcgtgg gccggccgac gccggagggc
    19981 gcgcatcgcg acggcgtgga tttcgtcgcg gtcgtgctgg tggcgcgcaa tggcatcaag
    20041 ggtggcgaaa cgcgggtatt ccaggccgat ggcccggatg gccagcgctt caccctggcc
    20101 gagccgtggt cgttgctgct gctggacgat gcgaaggtga tccacgaatc cacgccgatc
    20161 cagccgctcg acgggtatgg gcaccgcgat acgctggtgc tgacctaccg ggccgcgggc
    20221 ttccagggcg atgacgcggg ttgatcgccc gacacccgac tcatcgttac aattgcaact
    20281 atcgcaagca acgcaaatcc cgaggtcgct atgaaactgg gttctctcaa ggaaggcggg
    20341 cgcgacggca ccctcgtcgt ggtctcgcgc gacctcaccc gcgcggtgcg cgcgaccggg
    20401 attgcagcga cgctgcagca ggcgctggag gactggtcaa acaccgcgcc gcgcctcaat
    20461 gcgctgtcgg aggcgttgga cgacggctcc gctgacgcgg cgttcgacct cgacatgcag
    20521 gcgctggcgg caccgttgcc gcgcgcctac gaattcgtcg atggcagcgc ctacctgccg
    20581 cacgtggcgc gcgtgcgccg cgcgcgtggc gccgaggtgc cggacagctt ctacaccgat
    20641 ccgctgatgt accaggcggt cagcgccggc ttccttggcc cgcgcgatcc ggtgagggtg
    20701 gtcagcgagg actatggcat cgacctcgag gccgagatcg tgatcgtcac cgacgacgtg
    20761 ccgatggcgg
//
LOCUS       DAORUK010000021        18867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_2551_length_18867_cov_19.944397, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000021 DAORUK010000000
VERSION     DAORUK010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 18867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18867
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2551_length_18867_cov_19.944397"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..837)
                     /locus_tag="PK818_02645"
     CDS             complement(166..837)
                     /locus_tag="PK818_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQY81666.1"
                     /translation="MTQDLDQTEKKDDREGGLSWPRIAGLSFAVALHAAALLLLLAPI
                     SPPAQDVEEDDVVSVTFIEPPPPPPPPPPPPPDQPKPLTPPKTLAPPRPSPLPPPPDD
                     PPVILDTPRAVDVQAPPPAPPAPPSSVPDIGSSVDASSRAMNPPKYPPEEQRRGIQGT
                     TVLIVSIDPSGGVLDVEVEKSSGNRNLDRAAVAAARRWRFNPEVRDGRKIASRVRVPV
                     DFTLR"
     gene            complement(987..2120)
                     /locus_tag="PK818_02650"
     CDS             complement(987..2120)
                     /locus_tag="PK818_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81667.1"
                     /translation="MNRILQGRALTLAAGAALGLLALAPIAEAQAQSSTPSMREQRAK
                     RMAELGKDKDQAKQAEQKPALYPNATRVSPDAKASGKTVKQLQALQELYEKSDWAGVI
                     AKAEQVAAMPIAGPYEKSFAYSMAGNASADLDDQARAADYFAKAVAADGLDNDSHYNT
                     MYNLAVIQFGNENYAGALATIDRFLAETKSDKPDRLAFRAGILASLGRNDEAGAIYKD
                     LVAKNPDDKRLLMNAVAAFQQADKFDQANLLLEDAYKRGMLSEARELRALYIGYMNAQ
                     RWVDAQKVIDDGLAKGILQSGPELARDYQVLAQNAYVDDKIALAIELYTKAAPMAADG
                     EAYLNLAKVLEYNGKKADAKAAAQKALDKGVKKPDEARNILSR"
     gene            complement(2307..2410)
                     /locus_tag="PK818_02655"
                     /pseudo
     CDS             complement(2307..2410)
                     /locus_tag="PK818_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016901838.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
     gene            complement(2522..5014)
                     /gene="gyrB"
                     /locus_tag="PK818_02660"
     CDS             complement(2522..5014)
                     /gene="gyrB"
                     /locus_tag="PK818_02660"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HQY81668.1"
                     /translation="MSQNESNPGQVPESPAPTSPSSSAPANNGTYDSSKITVLRGLEA
                     VRKRPGMYIGDVHDGTGLHHMVFEVVDNSVDEALAGHANDIFVTIHEDGSVSVSDNGR
                     GIPVDIHKEEGVSAAEVILTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLTLDI
                     WRDGGHHQQEYALGEPVHPLRRLADSDRRGTLLRFWPATEIFSDVEFHYDILAKRLRE
                     LSFLNSGVRITLVDERGEGKRDVFEYEGGIRSFVEHLAQLKTPLHPNVISVSGEQNGI
                     TVDVALQWTDSYQETMFCFTNNIPQKDGGTHLIGFRAALTRTLGNYIEQNGIARQAKI
                     TLSGDDMREGMIAVLSVKVPDPSFSSQTKEKLVSSDVRPVVEHTFGARLEEFLQEHPN
                     EARAIAGKIVDAARAREAARKARDLTRRKGALDIAGLPGKLADCQEKDPALSELFIVE
                     GDSAGGSAKQGRNRKTQAILPLKGKILNVERARFDRMLASAEVGTLITALGTGIGKDE
                     YNPDKLRYHRIILMTDADVDGSHIRTLLLTFFYRQMPELIERGHIYIGLPPLYKIKQG
                     KNELYLKDDAALDAYLAANAVEGAQLLPALGEPAIEGAALESLLLAFANARGAIARNA
                     HRFDPIVLESLIDFTPLDAASLAGHADEAHELEALAVKLNRTGLGRPRYALRLQPANE
                     TRNAALLITRRHMGEELVQVLPLSIFEHGELKPLREAAAVLHGLIREGAQIVRGNKAQ
                     PVASFAEAQAWLLEEAKKGRQIQRFKGLGEMNPEQLWDTTVNPETRRLLQVRIEDAVA
                     ADAIFSTLMGDVVEPRREFIEDNALKVANLDV"
     gene            complement(5125..6225)
                     /gene="recF"
                     /locus_tag="PK818_02665"
     CDS             complement(5125..6225)
                     /gene="recF"
                     /locus_tag="PK818_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019336834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HQY81669.1"
                     /translation="MEIERLRLQGFRRFGEVELQPRPGFNLITGDNGSGKSTLLEALH
                     LLAHGRSFRGRVRDGLIRHGDPALGVYAEWRLGSGSLHRAGLRHSGSEWEARLDGGAV
                     GHLGELCEALAVVSFEPGSHALINGPSEGRRRYLDWGLFHVERAERGGDFLMLWRRHA
                     RALKQRNALLRSRRVDAQLEAWEHELAQAGEALNTQREAHVARLQPHLDAMVAALLPA
                     AGTATLGLNPGWRRQDLSLADALLLARDRDLAQGHTSMGPHRADLRLEFRDLPGREGL
                     SRGQAKLAALALLLAQAAYLAAQDGHWPVLQLDDIASELDRHHQRRVLQALHASGAQV
                     FITGTETPAAVQELGLPTTRFHVEHGRVEALG"
     gene            complement(6311..7411)
                     /gene="dnaN"
                     /locus_tag="PK818_02670"
     CDS             complement(6311..7411)
                     /gene="dnaN"
                     /locus_tag="PK818_02670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HQY81670.1"
                     /translation="MRFSLQREVLLKPLAQVVNVVERRQTLPVLANLLARVRDGQLSL
                     TGTDLEVEMVARTAVEDAQDGEITIPARKLFEIVRALPDGSRVTVSQSGDKVSVQAGR
                     SRFSLASLPANDFPSVDEVEATESVSVPEAALKELIERTAFAMAQQDVRYYLNGLLFD
                     LRDGALRCVATDGHRLALCEAALEGGHGSKRQIIVPRKGVQELQRLLEGGDRTLELEL
                     GRSHIRVKRDDVTFTSKLIDGKFPDYEAVIPIGADREVKIDREVLRAALQRASILSNE
                     KYRGVRVEVSPGQLRISSNNPEQEEAQEEVEADTHVDALAIGFNVNYLLDALSALRDE
                     YVVLQLRDANSSALVRGADSQHARHVVMPLRL"
     gene            complement(7665..8993)
                     /gene="dnaA"
                     /locus_tag="PK818_02675"
     CDS             complement(7665..8993)
                     /gene="dnaA"
                     /locus_tag="PK818_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011050021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HQY81671.1"
                     /translation="MDVWPRCLQRLEAELPQEDVRTWLKPLQADRRDRQLVLYAPNAF
                     VMEEVRSHYLPRIRELAAHFGDAEVSLDIGAMRRAEPAVPATGTGVRAAVASEPFQGN
                     VDPHYTFDNFVEGRSNQLGRAAAWQAALKPGDRAHNPLLLYGGTGLGKTHLMFAAGNQ
                     MRAVNPAARVMYLRSEQFMNAFTKALYDKSSGGMDAFKRQFQGIDALLIDDIQFFAGK
                     DRTQEEFFHTFNALFDGRQQIVLTCDRYPREVDGLEPRLKSRLGWGLSVAIDPPDFET
                     RAQIVLAKARERGTEVPADVAQLIAKKMRSNVRELEGAINKLVAEAGFAGRALTLEFA
                     QETLRDLLRAQQQAISIANIQKVVADYYGLQLREMLGKKRTRSLARPRQMAMALAKEL
                     TEHSLPEIGDAFDGRDHTTVLHACRQIRHLIESDGKLHEDWEKLIRKLSE"
     gene            9220..9360
                     /gene="rpmH"
                     /locus_tag="PK818_02680"
     CDS             9220..9360
                     /gene="rpmH"
                     /locus_tag="PK818_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HQY81672.1"
                     /translation="MATKRTYQPSNLKRKRDHGFRARMKTADGRKILARRRAKGRSRL
                     TV"
     gene            9416..9841
                     /gene="rnpA"
                     /locus_tag="PK818_02685"
     CDS             9416..9841
                     /gene="rnpA"
                     /locus_tag="PK818_02685"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014505377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HQY81673.1"
                     /translation="MPAPATGAGIVVPASFPRTARVRAKVEFDRVFEAGRRTAEPLFA
                     LHRLDDGAPPRLGLAVSRKVDPHAVGRNRIKRVLRDAFRHARGQLGPGAYVLVARPPA
                     AKADNHALRAAFLRLLQRAGALPMPAVAGTMPPASSPSS"
     gene            9879..11519
                     /gene="yidC"
                     /locus_tag="PK818_02690"
     CDS             9879..11519
                     /gene="yidC"
                     /locus_tag="PK818_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HQY81674.1"
                     /translation="MNQTRTFLLFAWLMVAALLWMEWNKERPVPAGAAVAPSAQPAAA
                     MPAVPAAQVAGASNAAAPVVASAVPAPVLTLKNDVLALRLDGRGVIGAQLLQYPQARQ
                     PGSPAVVLFDPAPARFFAARSGWVALDGGAEPAFRPETGAREAQLADGADTVSVRFVA
                     TGGNGLQLRRSYSLARGSYALQVRDELVNSGAAPWSGMVYRQLLRNPPAAKGGFTNPE
                     AFSLSGAAWYSPEDKYEKRKYPDFVEDGPLDQQVTGGWVALSQHYFLTAWVPQKDQGA
                     LYSLGVEGGLHRIEARGPQVSLAPGQRFTSDATLWVGPKLAEQLDATAPGLGLALDYG
                     MFTVLSKPIHWLLTQLHKLTANWGWAIVLLVVLIKLALYPLSAAQYKSMAKMRKFQPR
                     IEQLKERYGDDKQKFQMAMMELYKKEKINPAGGCLPILVQMPVFLALYYVLLEAVELR
                     QAPWIMGWVGDLTAQDPFFILPILNGAIMWATQKMTPMVGMDPMQKKMMQTMPLIMAV
                     MFAFFPAGLVLYWVTNGALGMLQQWWNTKRYAEEPAKA"
     gene            11639..12976
                     /gene="mnmE"
                     /locus_tag="PK818_02695"
     CDS             11639..12976
                     /gene="mnmE"
                     /locus_tag="PK818_02695"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011349216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HQY81675.1"
                     /translation="MNTNTETIVAIASAPGAGGVGIVRLSGPQARLIAETVCTRPLAP
                     RIAHYARFRDADGTLLDDGIALYFAGPASFTGEDVAELQAHGSPVVLRQLVARCVALG
                     ARQARPGEFSERAFLNGKLDLAQAEAIADLIAAGDVQAARAARRSLDGVFSRRVAALM
                     EALLAIRVHVEAAIDFADEPLETLGGADLRARLDAAIAQLAQLQREAARGQKLRDGLH
                     VVLVGPPNAGKSSLLNALAGSERAIVTEHAGTTRDLLRETIRIDGVELTLVDTAGLRE
                     AGDAIEAEGIRRARSEAGRADLALAVLDARDPGAGRAAVADGIAGVPQVLWLHNKADL
                     LAPGPQPAADDVLQVSAKTGLGLDQLHARLLALSGAGDPGEGSFSARARHVQALALAH
                     EQLGRAGAELQVERLELAAEALARAHAALGEVGGRLSADGLLGHIFSSFCIGK"
     gene            13063..14109
                     /locus_tag="PK818_02700"
     CDS             13063..14109
                     /locus_tag="PK818_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQY81676.1"
                     /translation="MSAINKMRRSFAPVALAVAVACALGACKKDDAAADAGAAAAQQA
                     PAPTPESVVSATVTAMSPEQLRDEASKAYGENRLYAPAGNNAMEYYLALRDKQPADAG
                     ASSALTDLLPMTVIATEQGIAREDFADAKRLAALIEKADAKHPALSRLKAAIASSETA
                     AAARVESQKLTAEEEAKRQEELAKKREEDQRKLQEQQKQQAAAQAPAAATPAAPAAAA
                     AEAERQRAAAAEAERERAAAAAAEQQRQQAAAQQPAAQPARAASSELRAISNPGPRYP
                     QAAQRAGAGGSVQVEFTVNTDGSVGGVRAVSSDGPRQFQRDFEREALAAVKRWRFQPV
                     SEATTTRRTIAFQQ"
     gene            complement(14184..15374)
                     /locus_tag="PK818_02705"
     CDS             complement(14184..15374)
                     /locus_tag="PK818_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQY81677.1"
                     /translation="MNVHTRSMIVVMRKELRDFYRDRRTFLMTLLLTPLLFPAIMLGM
                     FKLMENRANTQLDKDMAIPVAGGEFAPNLLAHLASHGIAARKASVAEVEQAVRSQVDD
                     VGLVIDPGFAKDWHSGAPAKVELVTDSTRRNADVPVARVEATLKQYSDTLGALRMLAR
                     GINPMVAVPVGVGTRDLATPEAKRGIMLSAMLPILLLISAFLGGIHLAMDTTAGERER
                     QSLEPLLATPAARGAIVSGKMLASVLIGIASLLLILLALKGSAAMATGMGRMLDVSLP
                     AIAKLLLILLPLVVIGTGLITFVAAGAKSMKEAQSHVMWMMMLPMIPTYALMASPLKD
                     TDLWQYAVPFLSQNQLVQKVTRGESPDPQQWAVYLGCALALAAALWMAAVWRYRQEKL
                     AISA"
     gene            complement(15371..16126)
                     /locus_tag="PK818_02710"
     CDS             complement(15371..16126)
                     /locus_tag="PK818_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY81678.1"
                     /translation="MIVAEHLRKTFPGKGKKAAPVIAVDDVSFTAHDGQITGLLGPNG
                     AGKTTTMRMLYTLMQPEAGRVLVDGIDVGRDAEAVRRSLGVLPDARGVYKRLTARENI
                     AYFGELHGMGAAQVAERTRKLAEALQMEDVLDRECEGFSQGQRTKTAIARALVHDPRN
                     VILDEPTNGLDVMTTRGLREFLKQLRAEGRCVIFSSHIMQEVAALCDRIVIIANGRVM
                     AEGTPGELRAKAGIDNLEDAFVRLIGSDEGLHA"
     gene            complement(16191..17699)
                     /locus_tag="PK818_02715"
     CDS             complement(16191..17699)
                     /locus_tag="PK818_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQY81679.1"
                     /translation="MTTRTLMLALAISATLLAGCKQQAAGSPATGDAARLRSLGSLQF
                     KPCSLASPLPNGKPLDAQCTTLAVPEDRSRQGGRKIDLNIAWIPPRNTSDPAADPVFF
                     LAGGPGQAAVATFPAMAPVFKDVMKDRGVVLVDQRGTGKSHPLNCKSAGSEQNGSDPA
                     AARAWIEACIAELSPKADLRRYTTTDAVADLEAVRKAIGADRINLMGVSYGTRMAQQY
                     ALRHPQHVRTVTLDSPVPNTLGLGNIFAGNLDSALQAQFALCKESPTCKQRMGDPRAE
                     LQSVLARLRANPVPVTYRDGSTGEEVTETITADHVAGLVRMYAYMPAVGALLPQLIRE
                     ASQGRYANLMALAKMMQGDLEESMSMGMQMSVICTEDAASMVVRAEDADTVLGNRMVE
                     SMASMCQAWPKGDKPADFNTALEGDLPVLVLTGEFDPVTPPKYGEEIAKSLPKSRWLD
                     LKGQGHSVLGAGCMPKLFAQFIEKADARALDAKCLDKLAYVPPFTSYNGWEP"
     gene            complement(17726..17920)
                     /locus_tag="PK818_02720"
     CDS             complement(17726..17920)
                     /locus_tag="PK818_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQY81680.1"
                     /translation="MNNRMRELREAQGWSQGELAERLEVSRQTVNALETGKYDPSLPL
                     AFRIARLFGESIESLFIPED"
     gene            complement(17961..18557)
                     /locus_tag="PK818_02725"
     CDS             complement(17961..18557)
                     /locus_tag="PK818_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81681.1"
                     /translation="MTMRRFMWIFVAIGLGSLALAGAGRFLLAWPDRMVGMWTGIGAG
                     YLLGASLFLLMPRWWKSHCDEMYAQPAGRRYLRALWPALVAYSLALVASIWWIRLGIE
                     SLPLRGLVAVLPVLPIAWMMHAALRYLREIDELQRRIETEAIGAASLLVALVYFAGGM
                     LEKAEVVRMDAGVAMIWVFPMLCGLYGIAKMVLTRRYQ"
     gene            complement(18559..18726)
                     /locus_tag="PK818_02730"
     CDS             complement(18559..18726)
                     /locus_tag="PK818_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81682.1"
                     /translation="MEQRKNLKGAGAAFLGSGVAFLAASLLAEQPAFIGVACAFLALG
                     VVYLGKARQDR"
BASE COUNT         2823 a         6408 c         6563 g         3073 t
ORIGIN      
        1 ggtcaaagcc catctgctgc agcgcggcgg cgttgttgcc tccggcggcg gcaggggcgg
       61 cagcagtcga ttcctgcaac atacgcttac ctttgggtgt cgtggatgga gtttagaagc
      121 ggaacaaggt gatcgggtgg tacgggtgca gcaaaatctg gtcgcttagc gcaaggtgaa
      181 gtcgaccggg acgcgcacgc ggctggcgat cttcctgccg tcgcgtactt ccgggttgaa
      241 acgccagcgg cgcgccgcgg cgaccgccgc gcggtcgagg ttgcggttgc cgctggactt
      301 ctcgacctcg acatcaagca cgccgccgga cggatcgatg ctcacgatca gcacggtggt
      361 gccctggatg ccgcgacgct gttcttccgg cgggtacttc ggcgggttca tcgcccgcga
      421 ggacgcgtcg acgctggagc cgatatccgg caccgacgac ggcggggccg gcggagccgg
      481 aggcggggcc tgcacgtcga ccgcgcgcgg cgtgtcgagg atcaccggcg gatcgtccgg
      541 cggcggcggc agcgggcttg ggcgcggcgg tgccaaggtc ttcggcgggg tcagcggctt
      601 gggctggtcc ggcggcggcg gcggcggcgg cggcggcggc ggcggcggct cgatgaaggt
      661 gacgctgacc acgtcgtctt cctcgacatc ctgcgccggc ggtgaaatcg gcgccagcag
      721 cagcagcagg gccgcggcat gcagggcgac ggcgaagctg agaccggcga tgcgcggcca
      781 gctcaagccg ccttcgcggt cgtctttctt ctcggtttgg tcgagatctt gcgtcatgcg
      841 cacggctctg gtgtgggaag ccccggacgc agggcgccag ggcaacaaca tgcggcccgg
      901 gggccgcagg aacctcctag tctataccaa tcccctgcgc ttgttccaag cgggaaattc
      961 aggcagacga aaatgaggcg catgtctcag cgggaaagga tgttcctggc ttcgtccggc
     1021 ttcttgacgc ccttgtcgag cgccttctgc gccgcggcct tggcgtccgc cttcttgccg
     1081 ttgtattcca gcaccttggc caggttgagg taggcctcgc catcggcggc catcggcgcg
     1141 gccttggtgt acagctcgat cgccaacgcg atcttgtcgt cgacgtaggc gttctgcgcc
     1201 agcacctggt agtcgcgggc gagctccggc ccggactgca ggatgccctt ggcaagcccg
     1261 tcatcgatca ccttctgcgc atcgacccag cgctgcgcat tcatgtagcc gatgtacagc
     1321 gcgcgcagct cgcgggcctc gctgagcatg ccgcgcttgt aggcatcctc cagcagcagg
     1381 ttggcctggt cgaacttgtc cgcctgctgg aaggccgcca ccgcgttcat cagcagccgc
     1441 ttgtcgtccg ggttcttcgc gaccaggtcc ttgtagatcg cccctgcctc gtcgttgcga
     1501 ccgagactgg cgaggatgcc ggcacgaaac gccaggcggt cgggcttgtc gctcttggtc
     1561 tcggcgagga aacggtcgat cgtcgccagc gcgccggcgt agttctcgtt gccgaactgg
     1621 atcacggcga ggttgtacat ggtgttgtag tggctgtcgt tgtccagccc atcagcggcc
     1681 accgccttgg cgaaatagtc ggcggccctg gcctggtcgt cgagatctgc cgaagcattg
     1741 ccggccatcg agtaggcgaa cgacttctcg tagggaccgg cgatcggcat cgccgccacc
     1801 tgctccgcct tcgcgatcac cccggcccag tccgacttct cgtacagctc ctgcagcgcc
     1861 tgcaactgct tgacggtctt gccgctggcc ttggcatcgg gcgaaacccg ggtcgcattc
     1921 gggtacagcg ccggcttctg ctcggcctgc ttggcctggt ccttgtcctt gccgagttcg
     1981 gccatgcgct tggcgcgctg ctcgcgcatg ctcggggtcg aggactgggc ctgggcctcg
     2041 gcgatgggtg cgagcgccag caggccgagg gcggcacccg cggcgagggt gagggcgcgg
     2101 ccttggagga ttcgattcat cggatcacct ctggaacggg agacgggaca gccggcatcg
     2161 gtcccggcag gggtcgaacc ctagcagaaa gcgggtggtc gcggccatgc cgggtaccgc
     2221 cgcacttggg cggcaggcga ctggcaacgc ggtctgccgg cctgcccgag tgccgcctcc
     2281 actaccggaa ccagggcctg cgcgcgcgcc tgcagtcgcg caggcgcgac gccgggtccg
     2341 ggtgggtgga cagccattgt ggcggccgcg agccgccggc cggggccatg ttccgccaaa
     2401 gtgcgatcgc cgcgttgagc aacaggacac gggtcgtcat cacggagccg gtacagggag
     2461 ggatgcctcc ccgtacggcg ggcaacctga ggttggcgtc aagccgccct gtaccccggc
     2521 ctcacacgtc gaggttggcg accttcagtg cgttgtcctc gatgaactcc cggcgcggct
     2581 ccaccacgtc gcccatcagg gtgctgaaga tcgcgtcggc ggcgaccgcg tcctcgatcc
     2641 gcacctgcag caggcggcgc gtttccgggt tcaccgtggt gtcccacagc tgctccgggt
     2701 tcatttcgcc caagcccttg aagcgctgga tctggcggcc cttcttggct tcctccagca
     2761 accacgcctg cgcctcggcg aagctcgcga ccggctgcgc cttgttgccg cggacgatct
     2821 gggcaccctc gcggatcagc ccgtgcagca ccgccgcggc ttcacgcagc ggcttgagct
     2881 cgccgtgctc gaagatcgac agcggcagca cctgcaccag ttcctcgccc atgtgccggc
     2941 gggtgatcag cagcgccgcg ttgcgggttt cgttcgccgg ctgcagcctg agcgcatagc
     3001 gcggccggcc aagcccggtg cggttcaatt tcaccgccaa tgcctcgagt tcatgcgcct
     3061 cgtcggcgtg cccggccagc gaggccgcgt ccagcggggt gaagtcgatc agcgactcca
     3121 gcacgatcgg gtcgaagcgg tgcgcattgc gcgcgatcgc gccgcgtgcg ttggcgaatg
     3181 ccagcaacag cgactccagc gccgcgccct cgatcgccgg ctcgccgagc gccggcagca
     3241 gctgcgcgcc ctccaccgcg ttggcggcga ggtaggcatc aagcgccgcg tcgtccttca
     3301 ggtacagctc gttcttgccc tgcttgatct tgtacagcgg cggcaggccg atgtagatgt
     3361 ggccgcgctc gatcaactcc ggcatctgcc ggtagaagaa ggtcagcagc agggtgcgga
     3421 tgtgcgagcc gtcgacgtcg gcgtcggtca tcaggatgat gcggtggtag cgcagcttgt
     3481 ccgggttgta ctcgtccttg ccgatgccgg tgcccagcgc ggtgatcagg gtgccgacct
     3541 cggcgctggc cagcatgcgg tcgaagcggg cgcgttcgac attcaggatc ttgcccttca
     3601 acggcaggat cgcctgggtc ttgcggttgc gaccctgctt ggcggagccg ccggccgaat
     3661 cgccctcgac gatgaacagc tccgacagcg ccggatcctt ttcctggcag tcggccagct
     3721 tgcccggcaa gccggcgata tccagcgcgc ccttgcggcg ggtcaggtcg cgggccttgc
     3781 gcgcggcctc gcgcgcgcgc gcggcatcca cgatcttgcc ggcgatggcg cgcgcttcgt
     3841 tggggtgttc ctggaggaac tcctccaaac gtgcaccgaa cgtgtgctca acgaccggtc
     3901 tcacatcgga cgagaccagc ttttccttgg tctggctgga gaagctgggg tccggcacct
     3961 tgaccgacag caccgcgatc atgccttcgc gcatgtcatc gccggacagg gtgatcttgg
     4021 cctgcctggc aatcccgttc tgttcgatgt agttgcccag ggtgcgcgtc aatgccgcgc
     4081 gaaagccgat caggtgggtg ccgccgtcct tctgcgggat gttgttggtg aagcagaaca
     4141 tcgtttcctg gtagctgtcg gtccattgca gggcgacatc gacggtgatc ccgttctgct
     4201 cgcccgatac cgagatcacg ttcggatgca gcggggtctt cagctgggcc aggtgctcga
     4261 cgaagctgcg gatgccaccc tcgtactcga agacgtcgcg cttgccctcg ccgcgttcgt
     4321 cgaccagggt gatgcgcacc ccggaattga ggaacgacag ctcgcgcagg cgcttggcga
     4381 ggatgtcgta gtggaactcg acgtcgctga agatctcggt ggccggccag aagcgcagca
     4441 gggtgccgcg cctgtcggaa tcggccagcc gcctgagcgg atgcaccggc tcgcccagcg
     4501 cgtattcctg ctggtggtgg ccgccatcgc gccagatgtc cagggtgagc ttgctggaca
     4561 gcgcgttgac cacgctgacg cccacgccat gcaggccgcc ggacaccttg tagctgttgt
     4621 cgtcgaactt gccgccggca tgcagcacgg tgaggatcac ctccgccgcg gacaccccct
     4681 cttccttgtg gatgtcgacc ggaatgccgc gcccgttgtc ggacacgctg accgagccat
     4741 cctcgtggat ggtgacgaag atgtcgttgg cgtgcccggc cagcgcctcg tccaccgaat
     4801 tgtcgaccac ctcgaacacc atgtggtgca ggccggtgcc gtcgtggacg tcgccgatgt
     4861 acatgcccgg ccgcttgcga acggcttcca gcccgcgcag gacggtgatc ttgctggaat
     4921 cgtaggtccc gttgttggcg ggcgcgctgc tgctcgggga cgtgggggcg ggggactcgg
     4981 gcacctggcc cggattggac tcgttctgcg acatcgacgc gttcagctcc cgcggcgcgc
     5041 agagggactc gatgcgcgcc gagacacatt agctatagga ggccgcagta tagcaaccgg
     5101 cgcccggcga cgcctgccaa ggcctcagcc gagggcttcg acgcggccgt gttccacgtg
     5161 gaaccttgtg gtcggcagcc ccagttcctg cacggcggcc ggcgtctcgg taccggtgat
     5221 gaagacctgt gcgccgctgg cgtgtagcgc ctgcagcacg cgccgctggt gatggcggtc
     5281 gagttcagag gcgatgtcgt ccagctgcag caccggccag tgcccgtcct gggccgcgag
     5341 gtacgcggcc tgtgccaaca acagggccag cgcagccagc ttggcctgac cgcgcgacag
     5401 cccctcgcgg ccgggcaggt cccggaactc caggcgcagg tccgcccggt gcgggcccat
     5461 cgaggtatgg ccctgggcaa ggtcgcggtc gcgtgcgagc agcagcgcgt cggcaagcga
     5521 caggtcctgc cgacgccaac ccggattcag gccgagcgtg gcggttcccg cggctggcaa
     5581 cagcgccgcg accatcgcat cgaggtgcgg ctgcaggcgc gcgacgtggg cctcgcgctg
     5641 ggtgttgagc gcttccccgg cctgcgcgag ttcgtgctcc caggcctcga gttgcgcgtc
     5701 cacgcgccgc gaccgcaaca gcgcattgcg ttgcttcagc gcgcgtgcat ggcgccgcca
     5761 caacatcagg aagtcaccgc cacgctcggc acgttccacg tggaacaacc cccagtcgag
     5821 gtagcggcgg cgaccttcgc tgggtccatt gatcaaggca tggctgccgg gttcgaagct
     5881 gaccaccgcc agcgcctcgc acagttcgcc caggtgtccc accgcgccgc catccagccg
     5941 cgcctcccat tcgctgcccg aatgccgcaa cccggcacga tgcaagctgc cggacccgag
     6001 ccgccattcc gcgtacacgc ccagcgcggg atcgccgtgg cggatcaggc cgtcacggac
     6061 ccgcccccgg aagctgcgcc catgcgccag caggtgcagc gcttccagca gggtggactt
     6121 gccgctgccg ttgtcgccgg tgatcaggtt gaagccgggg cggggctgca gctcgacctc
     6181 gccgaaccgg cggaaaccct gcaaccgaag tcgctcgatc tccaccctca ggtcgcccca
     6241 cgcgcaaaac ggcaacgccc ggcgggaacc gggcgttgct ggcgacgttc cacgtggaac
     6301 acgggcgcaa tcacagacgc agtggcatca ccacatggcg cgcgtgctgg ctgtcggcgc
     6361 cgcgcaccag cgccgacgaa ttggcatcgc gcagctgcaa gaccacgtac tcgtcgcgca
     6421 gggcggacag cgcatccagc aggtaattga cgttgaagcc gatcgccagt gcgtccacgt
     6481 gggtgtccgc ctcgacctct tcctgcgcct cttcctgctc cggattgttc gagctgatcc
     6541 ggagctggcc gggggacacc tcgacccgga ccccgcgata cttctcgttg gacaggatcg
     6601 atgcacgctg cagagccgca cgcagcacct cgcggtcgat cttgacctca cggtcggcgc
     6661 cgatcgggat caccgcctcg tagtcgggga acttgccgtc gatcagcttg ctggtgaagg
     6721 taacgtcgtc gcgcttgacc cggatgtggc tgcggcccag ttccagctcc agcgtgcggt
     6781 cgccgccctc gagcaggcgc tgcagctcct gcacgccctt gcgcggcacg atgatctggc
     6841 gcttgctgcc gtggccgccc tccagcgcgg cctcgcacag ggcaaggcga tggccgtcgg
     6901 tggcaacgca gcgcagcgcg ccatcgcgca ggtcgaacag cagcccattc aggtagtagc
     6961 ggacgtcctg ctgggccatg gcgaacgcgg tgcgctcgat cagttccttc aacgccgctt
     7021 ccggcaccga cacgctctcg gtggcctcga cttcgtccac cgacgggaaa tcgttggccg
     7081 gcaggctggc caggctgaag cggctgcgcc cggcctgcac gctgaccttg tcgccgctct
     7141 ggctgacggt gacccggctg ccgtcgggca gggcccgcac gatctcgaac agcttgcggg
     7201 cgggaatcgt gatctcgccg tcctgggcgt cctcaaccgc ggtccgcgcc accatttcca
     7261 cttcaaggtc ggtaccggtc agcgagagct ggccatcgcg caccctggcc aggaggttgg
     7321 ccagcaccgg cagggtctgc cggcgttcga ccacgttgac gacttgcgcc agtggcttca
     7381 gcaggacttc gcgttgcagg gagaaccgca tgttgacccc tagattccga tgcgtgaagg
     7441 ccggcccgtg cgtgctgtcc cggctcttgg aagaatggat aaatcagaaa agctggtgga
     7501 gatagtgcgc ttcaaaaccc gggaaaacac attcaaccat ttgtttcaaa aggatatttc
     7561 ctgaacggaa gcgggtggat aagctttcgc ggggttgcgg acaagttgtg gacaactttc
     7621 gtgcttgccg gcaggcgcct acttatccac agcttgtccg gcattcattc actgagtttg
     7681 cggatcagtt tttcccagtc ctcgtgcagc ttgccgtcgc tctcaatcaa gtggcggatc
     7741 tgccggcagg cgtgcagcac ggtggtgtgg tcgcggccat cgaacgcatc gccgatctcc
     7801 ggcaggctgt gttcggtgag ctccttggcc agggccatcg ccatctgccg cggccgcgcc
     7861 agcgagcggg tccgcttctt ccccagcatc tccctcagtt gcaggccgta gtagtctgcc
     7921 acgaccttct ggatgttggc gatgctgatc gcctgctgct gcgcgcgcag caggtcgcgc
     7981 agggtctcct gggcgaattc gagggtcagc gcgcgcccgg cgaagccggc ttcggcgacc
     8041 agcttgttga tcgcgccctc cagttcgcgc acgttgctgc gcatcttctt ggcgatcagc
     8101 tgggccacgt cggccgggac ctcggtgccg cgctcgcggg ccttggccag cacgatctgg
     8161 gcgcgggtct cgaagtccgg cgggtcgatc gccaccgaca gcccccagcc cagccgcgat
     8221 ttcagcctcg gttccaggcc gtcgacctcg cgcgggtagc ggtcgcaggt cagcacgatc
     8281 tgctggcggc cgtcgaacag cgcgttgaag gtgtggaaga actcctcctg ggtgcggtcc
     8341 ttgccggcga agaactggat gtcgtcgatc agcagcgcat cgatgccctg gaactgccgc
     8401 ttgaacgcat ccatgccgcc actggacttg tcgtacaggg ccttggtgaa ggcgttcatg
     8461 aactgctcgg agcgcaggta catgacccgt gccgccggat tgaccgcacg catctggttg
     8521 ccggcggcga acatcaggtg ggtcttgccg aggccggtgc cgccgtacag cagcagcggg
     8581 ttgtgggcgc ggtcgccggg cttgagcgca gcctgccagg cggctgcacg gccgagctgg
     8641 ttgctgcggc cctcgacgaa gttgtcgaat gtgtagtgcg ggtcgacgtt gccctggaac
     8701 ggttcgctcg cgaccgccgc gcgcacgccg gttccggttg ccggcaccgc gggttcggcc
     8761 cggcgcatcg caccgatgtc caggctgacc tcggcatcgc cgaaatgcgc ggccagctcg
     8821 cggatccgcg gcaggtagtg gctgcgcact tcctccatca cgaatgcgtt cggcgcatac
     8881 agcaccagct gccggtcgcg gcgatctgcc tgcaggggct tcagccaggt gcgcacgtcc
     8941 tcctgtggca gttcggcttc caggcgttgc aggcagcggg gccagacatc catgacgggc
     9001 ggtactctcg gggttgcgag gtcgacggcg ccaccgacgc cggcacggcc gcctgtggat
     9061 aaatcgggga accggcagcg taccaccccc catcgtgggc ggccatcggc gccgggctgg
     9121 cacccatgtc atcaggtgcg ggcaacgcgt gggttgaccg ggaatgcgct cgccgttatc
     9181 cttccgaatc ttttccgccg aaaaatacgc aggcccgcca tggcgaccaa gcgcacttac
     9241 caacccagca atctgaagcg caagcgcgac cacggtttcc gtgcccgcat gaagaccgcc
     9301 gatggccgca agatcctcgc gcgtcgccgt gcgaagggcc gcagccgcct gacggtctga
     9361 cccgcgtccg gccccggtgc cggatccgca cgtgagttcc aggtcgcaca catcgatgcc
     9421 ggcgcccgcc acgggcgccg gcatcgtcgt gcctgcgtca ttcccgcgga cggcgcgggt
     9481 gcgcgcgaag gtcgagttcg accgcgtgtt cgaagccggc cgccgcaccg ccgagccgct
     9541 gttcgcgctg caccggctgg acgacggcgc cccaccgcgg ctgggactgg cggtgtcgcg
     9601 caaggtcgat ccgcatgcgg tcggccgcaa ccgcatcaag cgcgtgctgc gcgacgcctt
     9661 ccgccacgcg cgcggccagc ttggtcccgg cgcctacgtg ctggtggcgc gtccaccagc
     9721 ggcgaaggcc gacaaccacg ccctgcgcgc ggccttcctg cgcctgttgc agcgcgccgg
     9781 tgcgttgccg atgccagccg tggccggcac aatgccgccc gcatcctccc cctcctcctg
     9841 aagcgccgcg cactgcgccg gccgagcctg cctccctgat gaaccagacc cgtaccttcc
     9901 tgttgttcgc ctggttgatg gtggcggccc tgttgtggat ggaatggaac aaggagcggc
     9961 cggtaccggc aggcgcggcg gtggcgccca gcgcccagcc cgcggcagcg atgccggcgg
    10021 tgccggcggc gcaggtcgcc ggggccagca acgccgcggc gccagtcgtt gccagcgccg
    10081 tcccggcacc ggtgctgacc ctgaagaacg atgtgctggc gctgcgcctg gatggccgcg
    10141 gcgtgatcgg cgcgcaactg ctgcagtatc cgcaggcgcg ccagcccggc agcccggcgg
    10201 tggtcctgtt cgacccggcg ccggcccgtt tcttcgccgc gcgcagcggc tgggtcgcgc
    10261 tggacggtgg tgcggaaccg gcattccgcc ccgagaccgg cgcccgcgag gcgcagctgg
    10321 ccgacggtgc ggatacggtg agcgtgcgct tcgtcgccac cggcgggaac ggcctgcagt
    10381 tgcgccgcag ctacagcctc gcgcggggca gctatgcatt gcaggtgcgc gatgagctgg
    10441 tgaattcggg tgccgcgccg tggtcgggca tggtctaccg ccagctgctg cgcaacccgc
    10501 cagcggcgaa gggcggtttc accaatccgg aggccttcag cctcagcggc gcggcctggt
    10561 acagcccgga agacaagtac gaaaagcgca agtacccgga tttcgtcgag gacggcccgc
    10621 tcgaccagca ggtcaccggc ggctgggtgg cgttgtcgca gcactatttc ctgaccgcct
    10681 gggtgccgca gaaggaccag ggcgcgctgt actcgctggg ggtcgagggc ggcctgcacc
    10741 ggatcgaggc gcgcggcccg caggtgtccc tggcgccggg ccagcgcttc accagcgacg
    10801 ccaccctgtg ggtgggcccg aagctggcgg agcagcttga cgccaccgcg cccgggcttg
    10861 gcctggcgct ggactacggc atgttcacgg tgctgtccaa gccgatccac tggctgctga
    10921 cccagttgca caagctcacc gccaactggg gctgggcgat cgtgctgctg gtggtgctga
    10981 tcaagctggc gctgtatccg ctgtcggccg cgcagtacaa gtcgatggcc aagatgcgca
    11041 agttccagcc gcgcatcgaa cagctcaagg agcgctacgg cgacgacaag cagaagttcc
    11101 agatggcgat gatggagctg tacaagaagg agaagatcaa cccggccggc ggctgcctgc
    11161 cgatcctggt gcagatgccg gtgttcctgg ccctgtacta cgtgctgctg gaggcggtgg
    11221 aactgcgcca ggccccgtgg atcatgggct gggtcggcga cctgaccgcg caggatccgt
    11281 tcttcatcct gccgatcctc aacggcgcga tcatgtgggc gacgcagaag atgacgccga
    11341 tggtcggcat ggatccgatg cagaagaaga tgatgcagac catgccgctg atcatggcgg
    11401 tgatgttcgc cttcttccca gccgggctgg tgctgtactg ggtcaccaac ggcgcgctgg
    11461 gcatgctgca gcagtggtgg aacaccaagc gctatgccga ggagccggcg aaggcctagc
    11521 cggcgcgttc ggatgcgtca ggcccgccac gggatgcctt ggtggagtga acggcagctc
    11581 tggtggtcct gcgtaccgac ccgccacatg ccaccttgtg ggttgcgatg gtcctgcgat
    11641 gaatacgaac accgagacca tcgttgccat cgccagcgcg cccggggcgg gcggggtcgg
    11701 tatcgtgcgg ttgtccgggc cgcaggcgcg gttgatcgcc gaaaccgtct gcaccaggcc
    11761 cctggcgccg cgcatcgcgc attacgcacg tttccgcgat gccgacggca ccctgctgga
    11821 tgacgggatc gcgctgtatt tcgccggtcc cgccagcttc accggcgagg acgtggccga
    11881 actgcaggcg cacggcagcc cggtcgtgct gcgccagctg gtggcgcgct gcgtcgcgct
    11941 gggtgcccgc caggcccgcc ctggcgagtt cagcgaacgc gcgttcctca acggcaagct
    12001 cgacctcgcc caggccgagg cgatcgccga cctgatcgcc gccggcgacg tgcaggcggc
    12061 ccgcgcggcc cggcgttcgc tggacggcgt gttctcgcgc cgggttgcgg cgctgatgga
    12121 ggcgctgctg gcgatccggg tgcacgtgga agcggcgatc gacttcgccg acgagccgct
    12181 ggagaccctt ggtggcgccg acctgcgcgc gcggctggat gccgcgatcg cgcagcttgc
    12241 gcagttgcag cgcgaggccg cgcgcggcca gaaactgcgc gatggcctgc acgtggtgct
    12301 ggtcggcccg ccgaacgccg gcaagagttc gctgctcaat gcgctggctg gaagcgagcg
    12361 tgccatcgtc accgagcatg cgggcaccac ccgcgacctg ctgcgcgaga ccatccgcat
    12421 cgatggcgtg gagctgaccc tggtcgacac cgccggcctg cgcgaggccg gcgacgcgat
    12481 cgaggcagag ggcatccgcc gcgcgcgcag cgaagccggg cgcgcggacc tggcgctggc
    12541 ggtgctggat gcgcgcgacc ccggggcagg gcgcgccgcg gtcgcggacg ggatcgccgg
    12601 ggtgccgcag gtgctgtggc tgcacaacaa ggccgacctg cttgctcccg gcccgcagcc
    12661 ggcggcggac gacgtgctgc aggtctcggc gaagaccggg cttggcctgg accagttgca
    12721 tgcgcggctg ttggcgcttt ccggcgccgg cgacccgggc gagggcagct tcagcgcccg
    12781 tgcccggcac gtgcaggcgc tggcgctggc gcacgaacaa ctggggcggg cgggcgcgga
    12841 actgcaggtc gaacgcctgg aactggccgc cgaggccctg gcccgggccc atgcggcgct
    12901 cggcgaggtc ggcggccggc tcagcgccga tggcctactc ggtcacattt tcagcagttt
    12961 ctgcatcggc aaataggacg aattcgtcca cccacttgac aaatcggcgt cactgtcgcg
    13021 tgctatcgac gggtgcgttt gcatcaaacg gggaattcac gaatgtctgc gatcaacaag
    13081 atgcgccgaa gcttcgcgcc ggtggcgctt gcggtggcgg tggcgtgcgc gcttggcgcg
    13141 tgcaagaagg acgatgccgc ggccgatgca ggtgcggcgg ccgcgcaaca ggcaccggcg
    13201 ccgacgccgg agtcggtggt gtcggcaacg gtcacggcaa tgagcccgga gcagctgcgc
    13261 gacgaggcgt ccaaggccta tggcgagaac cgcctgtatg cgccggccgg caacaacgcg
    13321 atggagtact acctggcgct gcgcgacaag cagccggccg acgccggtgc atccagcgca
    13381 ttgaccgacc tgctgccgat gacggtgatc gccaccgagc agggcatcgc ccgcgaggac
    13441 ttcgcggatg ccaagcgcct ggccgcgctg atcgagaagg ccgacgccaa gcatccggcg
    13501 ctgtcgcgcc tgaaggcggc gatcgccagc agcgagaccg cggccgcggc gcgcgtggaa
    13561 agccagaagc tgaccgcgga agaggaagcc aagcggcagg aggaactggc gaagaagcgc
    13621 gaggaagacc agcgcaagct gcaggagcag cagaagcagc aggcggccgc gcaggcaccg
    13681 gcggcggcca ccccggcggc cccggcggcc gcggccgcgg aagccgagcg ccagcgcgcg
    13741 gcagcagcgg aggcggaacg cgagcgcgcc gccgccgccg ccgccgagca acagcgccag
    13801 caggccgcgg cacagcagcc ggcggcgcaa ccggcgcgtg cggcctccag cgagctgcgt
    13861 gcgatcagca acccgggtcc gcgttatccg caggccgcgc agcgcgccgg cgccggcggt
    13921 tcggtgcagg tggagttcac cgtcaacacc gatggcagcg tcggcggcgt gcgcgcggtc
    13981 agcagcgacg gcccgcgcca gttccagcgc gatttcgagc gcgaggcgct ggcggcggtg
    14041 aagcgctggc gcttccagcc ggtctccgag gccaccacca cccgccgcac gatcgccttc
    14101 cagcagtaac cgggcaggcg cgaaaacgaa aagcccggcg cgagccgggc tttttgttgc
    14161 ctgcaggcat gcgctggccg ggactaggcc gaaatcgcca gcttttcctg ccggtagcgc
    14221 cacaccgccg ccatccacag cgcggccgca agggcgagcg cgcagcccag gtagaccgcc
    14281 cactgctgcg ggtccggcga ctcgccgcgg gtgaccttct gcaccagctg gttctgcgac
    14341 aggaacggca ctgcgtactg ccagagatcc gtgtccttca gcggcgacgc catcagcgcg
    14401 taggtcggga tcatcggcag catcatcatc cacatcacgt ggctctgcgc ttccttcatg
    14461 ctcttggcgc cggccgccac gaaggtgatc aggccggtgc cgatgaccac cagcggcaac
    14521 aggatcaaca gcagcttggc gatcgccggc aggctcacgt cgagcatccg gcccatcccg
    14581 gtcgccatcg ccgcgctgcc cttcaacgcc agcaggatca acagcagcga ggcgatgccg
    14641 atcagcaccg atgccagcat cttgccgctg acgatggcgc cgcgtgcggc cggcgtggcc
    14701 agcaacggtt ccagcgattg ccgctcgcgc tcgccggcgg tggtgtccat cgccaggtgg
    14761 atgccgccca ggaacgcgga gatcagcagc aggatcggca gcatcgccga cagcatgatg
    14821 ccgcgcttgg cttccggcgt ggccaggtcg cgggtgccca cgcccaccgg caccgccacc
    14881 atcggattga tcccgcgcgc cagcatccgc agcgcaccga gggtatcgct gtactgcttg
    14941 agggtggcct cgacgcgcgc aaccggcacg tcggcgttgc gccgggtgga gtcggtgacc
    15001 agttccacct tggccggcgc gccggaatgc cagtccttgg cgaagccggg atcgatgacc
    15061 aggccgacgt cgtccacctg gctgcgcacg gcctgctcca cctccgccac gctggccttg
    15121 cgcgcggcga tgccgtggct ggccaggtgg gcgagcaggt tgggggcgaa ctcgccgccc
    15181 gccaccggga tcgccatgtc cttgtccagc tgggtgttgg cgcggttctc catcagcttg
    15241 aacatgccca gcatgatcgc cgggaacagc agcggggtga gcagcagggt catcaggaag
    15301 gtacggcggt cgcggtagaa atcgcgcagt tccttgcgca tcaccacgat catgctgcgg
    15361 gtatggacgt tcatgcgtgc aggccctcgt cgctgccgat gaggcgcacg aaggcgtcct
    15421 cgagattgtc gatgccggcc ttcgcgcgca gctcgccagg ggtgccttcg gccatcaccc
    15481 ggccgttggc gatgatcacg atgcggtcgc agagggcggc gacctcctgc atgatgtggc
    15541 tggagaagat cacgcagcgg ccctccgcgc gcagctgctt gaggaactcg cgcaggccgc
    15601 gggtggtcat cacgtccagc ccgttggtgg gctcgtccag gatcacgttg cgcgggtcgt
    15661 gcaccagcgc gcgggcgatc gcggtcttgg tgcgctggcc ctgcgagaag ccttcgcact
    15721 cgcggtccag cacgtcctcc atctgcagcg cctcggccag cttgcgggtg cgctcggcga
    15781 cctgcgccgc gcccatcccg tgcagttcgc cgaagtaggc gatgttctcg cgcgcggtca
    15841 ggcgcttgta cacgccgcgc gcgtcgggca acacgcccag gctgcggcgc accgcttcgg
    15901 catcgcggcc gacgtcgatg ccgtcgacca gcacgcggcc cgcctccggc tgcatcagtg
    15961 tgtagagcat gcgcatggtg gtggtcttgc cggcgccgtt ggggcccagc aggccggtga
    16021 tctggccgtc gtgggcggtg aagctgacgt catccacggc gatgacgggc gcggccttct
    16081 tgcccttgcc ggggaaggtc ttgcgcaggt gttctgcaac gatcatgggt gggtccttgt
    16141 ctgcgggggc gcgctctgaa ggggcgcggt catctggatt gccgcgcgca tcacggctcc
    16201 cagccgttgt acgaagtgaa cggcggcacg taggccagct tgtccaggca cttggcgtcc
    16261 agcgccctgg catcggcctt ttcgatgaac tgggcgaaca gcttcggcat gcagccggcg
    16321 cccagcacgc tgtggccctg gcccttcagg tccagccagc gcgatttcgg caagctcttc
    16381 gcgatctcct cgccgtactt cggcggggtc accgggtcga actcgccggt cagcaccagc
    16441 accggcaggt cgccctccag cgcggtattg aaatcggccg gcttgtcgcc cttcggccag
    16501 gcctggcaca tcgacgccat gctctcgacc atgcggttgc ccagcacggt gtccgcgtct
    16561 tcggcgcgca ccaccatgct ggcggcgtcc tcggtgcaga tcaccgacat ctgcatgccc
    16621 atcgacatcg attcctcgag gtcgccctgc atcatcttcg ccagtgccat caggttcgcg
    16681 tagcggccct ggctggcttc gcggatcagc tgcggcagca gcgcgccgac cgccggcatg
    16741 taggcgtaca tgcgcaccag tccggccacg tggtcggcgg tgatggtttc ggtgacttcc
    16801 tcgccggtgc tgccatcgcg gtaggtcacc ggcaccgggt tcgcgcgcag gcgggccagc
    16861 acggattgca gctcggcgcg cggatcgccc atccgctgct tgcaggtggg gctttccttg
    16921 cacagcgcga actgcgcctg cagggcgctg tccaggttgc cggcgaagat gttgccgagg
    16981 ccgagggtgt tcggcaccgg cgagtccagg gtgaccgtgc gcacgtgctg cggatgccgc
    17041 agcgcgtact gctgcgccat gcgggtgccg tagctgacgc ccatcaggtt gatcctgtcg
    17101 gcaccgatcg ccttgcgcac cgcctccagg tccgccactg cgtcggtggt ggtgtagcgg
    17161 cgcaggtccg ccttcggcga cagctcggcg atgcaggctt cgatccaggc gcgcgccgcc
    17221 gccgggtcgc tgccgttctg ctcgctgccc gcggacttgc agttgagcgg atgcgacttg
    17281 ccggtgccgc gctggtccac cagcaccacg ccgcggtcct tcatcacgtc cttgaacacc
    17341 ggcgccatcg ccgggaaggt ggccaccgcc gcctggccgg ggccgccggc caggaagaac
    17401 accgggtccg cggccgggtc gctggtgttg cgcggcggga tccaggcgat gttgaggtcg
    17461 atcttgcggc cgccctgcct gctgcggtcc tcgggcaccg cgagcgtggt gcactgcgcg
    17521 tccagcggct tgccgttcgg cagcggcgag gccagcgagc acggcttgaa ctgcaggctg
    17581 cccagcgagc gcagcctggc ggcatcgccg gtggccggcg aaccggcggc ttgctgtttg
    17641 cagccggcca gcagcgtggc cgagatggcc agcgccagca tgagggtacg ggtggtcatg
    17701 gtgaagctcc tgcgggatgc atggatcagt cttcggggat gaagagggat tcgatcgatt
    17761 cgccgaacaa ccgcgcgatg cggaaggcca gcggcagcga cgggtcgtac ttgccggttt
    17821 ccagtgcgtt gacggtctgc cgcgaaacct ccagccgttc ggccagctcg ccctgcgacc
    17881 agccctgcgc ctcgcgcagt tcgcgcatcc ggttgttcat gcgtgcccgc ggtgcagccg
    17941 cccacgcgac ccggcggcgc tcattggtag cgcctggtca ggaccatctt ggcgatcccg
    18001 tagagcccgc acagcatcgg gaacacccag atcatggcga cgccggcatc catgcggacc
    18061 acctccgcct tctccagcat gccgccggcg aaatacacca gcgcgaccag caggctggcg
    18121 gcgccgatcg cctcggtctc gatccgccgc tgcagttcgt cgatctcgcg caggtagcgc
    18181 aacgcggcgt gcatcatcca ggcgatcggc agcaccggca gcaccgcgac cagtccgcgc
    18241 agcggcagcg attcgatgcc cagccggatc caccagatcg aggcgaccag cgccagcgag
    18301 taggcgacca gcgccggcca cagcgcgcgc aggtagcggc ggccggcggg ctgcgcatac
    18361 atctcgtcgc aatgggactt ccaccagcgc ggcatcagca ggaacagcga ggcgccgagc
    18421 aggtagccgg cgccgatgcc ggtccacatg ccgaccatcc ggtccggcca cgccagcagg
    18481 aaacgacccg cgccggcgag ggcgagggag cccagcccga tcgccacgaa gatccacatg
    18541 aagcggcgca tggtcatttc agcggtcctg ccgggccttg cccaggtaca ccacgcccaa
    18601 tgcgaggaag gcgcaggcga cgccgatgaa ggcgggttgc tccgccagca gggaggccgc
    18661 gaggaaggcg acgccactgc cgaggaaagc ggcgccggcg cctttcaggt tcttgcgctg
    18721 ttccatgatt cgatcccaag tgtgtcaagg acacttgaca caacgatatc ggcatcacgc
    18781 acgccttgtc aagcgtcctt tacagctcgg cttacttgtt gctgacgtac ttctcgcggc
    18841 gcacctgagg caccggcagg ccggctc
//
LOCUS       DAORUK010000022        18731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_2594_length_18731_cov_19.247323, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000022 DAORUK010000000
VERSION     DAORUK010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 18731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18731
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2594_length_18731_cov_19.247323"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(478..1965)
                     /locus_tag="PK818_02735"
     CDS             complement(478..1965)
                     /locus_tag="PK818_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY81683.1"
                     /translation="MSELQDLLALIRAETPLLVIETPDESRVIALFRQSLTHAWRALH
                     RWSVTEGLRRLDIDREDESEGAPDLSATLQAIRSATQRGTYLLLDAHPYLGYASYQRA
                     LRDVIQRRDCQPHVLVMIGTRVELPPALEPFAVRFALRLPDANALLKLVQDEARAYAA
                     ENGGRRVEADAEAVKQIVRNLQGMSLTDARRIARHLIYRDGALGMGDLPELARLKFEL
                     LNKSGHLHYEYDTARFADVGGAARLKRWVEQRRLPFLQDPPPPGLDPPKGVLLLGVQG
                     CGKSLLAKAVAGGFGVPLVRLDFGTLYDKYHGESEKNLRAALASTEQLAPCVLWIDEI
                     EKGLAAGSGEGDGGLSRRVLGYLLTWMAERKSRVFLVATANQVHELPPELLRKGRFDE
                     IFFIDLPDAAARAEIFALHLARRNLPREGIDLPALAAAAEGYSGAEIEQVIVSALYAA
                     HAAQAGIDTDALLQAVRDTRPLSVLMAEQVQALRAWALPRTVPAD"
     gene            2011..4791
                     /gene="glnE"
                     /locus_tag="PK818_02740"
     CDS             2011..4791
                     /gene="glnE"
                     /locus_tag="PK818_02740"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HQY81684.1"
                     /translation="MADLPQLLERRLAQLPANAGWRADAAFMVKLRALAIASDFALAT
                     LVAQPALLERLHADDGLAPSPPPLLEAGNRADWPRLLRRHRHAESTRLVWRDVIAGDA
                     VDAILAGSTALAETCLQLALGALETEFAQRFGVLRDAGGQAQRLVVFGLGKLGGGELN
                     FSSDIDLVYAYEHDGESDGARGITASDYFARLGQQLAKLLDEVTADGFCHRVDLRLRP
                     FGNAGRVALSFAAMEQYFQREGRDWERYAWQKARPVAGDLAAGERFLAALRPFVYRRY
                     LDFGALDGLRTMKAAIAAEVARKELAGDIKRGPGGIREIEFLVQALQLIHGGREPALR
                     ERRLLPALQALVQAGHMAADAGAMLANAYRELRVLENRIQMLADAQTHALPAVPVQGE
                     RIARGLGHPGLAALDARLQAVRTRVAAEFAALLAPRGAVVGDSDLSLYWRSLPDDGDA
                     GALAAAGFGAAEELHRSLRDFARAPGVRELSDATRARLDRVLPALLQAASKSAQADPA
                     LRRLLPLLHTLLRRTSYLALLDEQPAALQRLVDALARSALLAERLGAHPLLLDELLDR
                     RVAGGLPDREAMQAQCAAALREGDAEASLHALNEVRQALSFRIALALLDARCDAQSCA
                     QRLAWLADAVVACVLRIAMGEMRAAHGVIPAGRFAVLGYGSLGGEELGFGSDLDLVFL
                     YDSADDAQSDGARPLDAPRWFARLGQKIVALLGVGTGAGRLFEVDVRLRPDGAKGLLV
                     SSLASFCDYQRERAWTWEHQALVRARAVAGDAELVADFDALRAQVLARPRDPASLAED
                     VSSMRRRMRAELDRSDAAVFDLKQGEGGLVDLEFLLQFLLLRDAAEHPALRVPRATPA
                     LLDAVLAAGSITPETHASLLAAHASLLDAGMRCTLDRRPRRVPPDAAIEAARAAIRAA
                     ISAQGLGETGSG"
     gene            complement(4842..5795)
                     /locus_tag="PK818_02745"
     CDS             complement(4842..5795)
                     /locus_tag="PK818_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HQY81685.1"
                     /translation="MSTTVAVHDGRAGNARQAVALARALDSDAGDCTLDPRAPWRWLA
                     PRVLPGADAAFGNAFKALAAQPPALAIGCGRQAALATRLLRDRGSQSVQILDPRIDPR
                     HWDLVIAPGHDALRGDNVIRTLGSLHPVDDLWLAQARRDFAHIATLPSPHTVLLVGGP
                     SRHVALQDAAFETLLRQLAGHARAVHGSFSVVASRRTPAHWRSMLAGMPAGLPGLHWR
                     DDRDGTNPYAGLLGHADRIVCTPDSVNMLSEAAATLAPVFMWCAGIANGRLRHFLDAL
                     RSSGRVRDLDDTLAPFAAEPLRETARVAGEVRLRLGEAGTA"
     gene            5912..6502
                     /locus_tag="PK818_02750"
     CDS             5912..6502
                     /locus_tag="PK818_02750"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HQY81686.1"
                     /translation="MTDALNDAALDQLFRSARTYNAFTGEVSDETLQQLYGLLKFGPT
                     EANTTPARIVFVKSAEAKARLAPALSEGNRAKTLAAPAVAIIGYDMRFFDKLPVLFPH
                     VDAKAWFEHRDEAGLAWVGLRNSSLQTAYLILAARSLGLDCGPMTGFDNAMVDAAFFA
                     GTAVRSNILVNLGQGDPASLYPRSPRLAFDEACQIA"
     gene            6543..7136
                     /locus_tag="PK818_02755"
     CDS             6543..7136
                     /locus_tag="PK818_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQY81687.1"
                     /translation="MSKRFLLTAALAAASANAFAAGVTYKLDPTHTNVIASWNHFGFS
                     NPSANFGGVEGTLVYDAADVARSSVQVTLPLSGMDSFVPKLDEHLRGPDFFDAARFPE
                     ARFSSTRVEAVAAGKLRITGDLTIRGVTRPLVLDATLNKAGEHPMLKVAAIGFDATAT
                     LKRSAFGLGGFAPAVSDEVTLRITTEASASAPAAAGK"
     gene            7144..7851
                     /locus_tag="PK818_02760"
     CDS             7144..7851
                     /locus_tag="PK818_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HQY81688.1"
                     /translation="MIVERPSAARGEVDLGWLRSRHTFSFGHYHDPAWMGFGALRVIN
                     DDTVAPGGGFAPHRHANMEIISVVLDGALAHKDSAGNEGVIRAGDVQWMSAGHGVEHS
                     EYNGSGTEPVHFLQVWIQPDRLNAQPAYAQQSFDPDARRARWATLFSADGADGSIAIR
                     QQASLRATRLHAGDVATAALDPARRYWLHVAAGGIEAGGRELQAGDALGFVDESGALP
                     LRGIGDRSDVLLFELPG"
     gene            7933..8127
                     /locus_tag="PK818_02765"
     CDS             7933..8127
                     /locus_tag="PK818_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HQY81689.1"
                     /translation="MTQPAPSPAKATQPHPVRRADLPLSCPLPSAALWNAHPRVFLPI
                     EADGGSSQCPYCGAAYVLVD"
     gene            8143..9261
                     /locus_tag="PK818_02770"
     CDS             8143..9261
                     /locus_tag="PK818_02770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY81690.1"
                     /translation="MRRLTVVQLLPALEAGGVERSTLEIAEALVRAGHRAIVVSAGGR
                     LVAPLEARGARHVTLDIGRKSPVTLRHVPALRRLLREAGADIVHARSRLPAWLGWLAL
                     RGMRAARPHFVTTAHGLNSPSRYSAIMARGERVVCVSETVRDYLQQHYPATDPRRLVV
                     IPRGIDPAAFPRAPLPDPAARARIAARHPQLGGRGPLLLLPGRGTRLKGHADAIALLA
                     DLRGSGLDARLWMPGAVQAGRAAYMAELAGLARVARVEAALAITEATADIAQAYAASD
                     LVLQLSRKPEAFGRTVIEAHAVGRPVLGWAHGGVGELLRALQPQGAVAPFDQALLAAT
                     ARRLLAQPQPPPAGTMQAFQLHAMQGATLALYQSLVGE"
     gene            9258..10550
                     /locus_tag="PK818_02775"
     CDS             9258..10550
                     /locus_tag="PK818_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQY81691.1"
                     /translation="MSATAAAPGWRWAPAWVVAFVALWPAPGVAEAVLALGAIAAVLH
                     LLLSRFRGSAALLTGPAWALTSVLFFAYWLPEAISAIDAADRARALREALVDLRYLPF
                     LWLVASAVTGAEGRRRTFGGLAVIVAAWTLDALLQAATGSSLLFAAIDGLKQAISGHG
                     MCTAAEVAALDRLSGVLGPCNLKLGIVLASLSPFALDAAGRRYGRIGWALAAVLVGLA
                     VLLAGSRAAWLSYCLVLAWTGWRLLGIKRLLAVFALGIALLLASALVSPPVAERINRS
                     TAVFVGNAEGVDHALSGRSRIWSAALCMARTHPINGVGARGFREAFPACDTEPGTRAA
                     WGDGPALHAHQVVLEVLSETGGIGLLLWLAGAAMAWRAWRYADAAARDQSRPAMLALA
                     VTVFPLNTHLAAYSTFWGGVLLLLAALYAGSLLGREPA"
     gene            10547..11305
                     /locus_tag="PK818_02780"
     CDS             10547..11305
                     /locus_tag="PK818_02780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY81692.1"
                     /translation="MRCRLSGVVTTLDNASTLEDCLASLAFCDEIVVLDSGSSDATLA
                     IAARHGARTASQAFAGYGPQKQAAIGLASGDWILLLDADEHLTAAGRSAIERELAAPR
                     ADGYRLPRREWLFWRWPHSATRANWQLRLFRKATGRMNDVPVHAAPEVAGRVLDLDAP
                     FRHYGEPRLADRVDKVNRYSSGLVEHKRGRRPRFIALRLLFYPGAAFLKLYLGKRYFL
                     NGWAGFFAAKTQSFYAFLKYAKVLEAARQDASRE"
     gene            complement(11284..12228)
                     /gene="lpxL"
                     /locus_tag="PK818_02785"
     CDS             complement(11284..12228)
                     /gene="lpxL"
                     /locus_tag="PK818_02785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HQY81693.1"
                     /translation="MSAAAPPDLPIPPRGLRHWPVWFGVAVCMGLARLPWPLQRAMGA
                     AVGWLALRLMPSRRRAARTNLALCMPELSTSERERLLRENFRDVGIGLFEFARAWWGD
                     AAPMRQTARIEGLDILGRLQAEGRGVLLVSGHFMTLEMCGRLLCDHVSLAGMYRRHRS
                     PVMEWAVLRGRLGYASAMFGNGDTRAAIRHLKRGGLLWYAPDQDMRGKDTVFAPFFGI
                     PAATITATHQFARLTGCAVVPFFHRRIPGKDGGDRYLLRIGEPLAPFPTDDAAADGSA
                     VNAAIEAMVRQAPSQYLWLHRRFKRQPDGVSRYSREAS"
     gene            12288..13592
                     /gene="waaA"
                     /locus_tag="PK818_02790"
     CDS             12288..13592
                     /gene="waaA"
                     /locus_tag="PK818_02790"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HQY81694.1"
                     /translation="MRADLTERVLRGLYSAALYILAPITLYHLIWRGFRQRAYFQRWN
                     ERYASYPAVTAATPLWLHAVSVGEVNAAAPLVTRLLQDHPGLRLVVTTITPTGSERVQ
                     ALWGDAVVHVYLPYDLPGAVARFLDHFRPALALIVETELWPNLLFACRDRGIPTHILN
                     ARLSARSLRGYRVLGPLIRRALRGVHLVAAQSDEDGKRFIRLGADPGRVVVTGNLKFD
                     TLPVDNTDFVREFRHHRGSGPTWIAASTHEDEEAAVLAAHHAVSQAHPDALLVWAPRH
                     PERFRAVAQRAAEAGFAVATRSEQGWPRNDARGRIQVFVVDTLGELTRFYACAQVAFV
                     GGSLQPVGGHNLLEPAATGTAILSGPQLHNFVDIARRLREAEAMRIVEDAPALASALL
                     ELFADADARERMAANAQQLLQAGRGALERTVALIEPGLPRRG"
     gene            complement(13600..14928)
                     /locus_tag="PK818_02795"
     CDS             complement(13600..14928)
                     /locus_tag="PK818_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014648347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HQY81695.1"
                     /translation="MLRRPLAFALALALMPAAAQADDLLQSYNSARNSDPQFAAAESS
                     RLVAAERPIQARAALLPQVGGSVGVSRVNQGGNSARTGSWGVDMSQSLFDYGNYTALR
                     AAKAQDRAGAFDLDAAGHNLITRTSAAYFNVLIQLESLAASEAAEAALKKQFDFASKR
                     LEVGLAPITDVHEARAQYDGARANTILARNAVQDAYQALVEITGTPVANLKGLPDDFK
                     PALPEARDVEGWVATALAENPSLKAQAADLEAAEANVETARSGHYPTLGLRASYGKAI
                     PGLYDPDYVGANKAATNVGLTLSVPIFSGLATQSGVRSALAQRDATQDAVEQTRRAIE
                     RSTRDAYQTLVAGISEVEARRLALFSAQSAYEASQVGLEVGTRTVLDVLDNQRTLFTA
                     QQVYARVKYNFLQNRLLLEQAAGTLGVSDVEDVNRLLTTDKAVGVATATP"
     gene            complement(14988..15641)
                     /locus_tag="PK818_02800"
     CDS             complement(14988..15641)
                     /locus_tag="PK818_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HQY81696.1"
                     /translation="MSIDYAKARETMVEQQVRPWDVLDPRVLETIGSVPREAFVPEAV
                     RALAYADTALPLGHGESMMKPVLEGRALQALRPGAEERVLEIGAGSGYLTACLARLAR
                     EVVAIELHADLADAARARLAAQGCGNARVETADALAWDNDQHFDAICVSGAVHALPPR
                     FVEWLKPGGRMFVVQGRSPAMQATLVHRDVNGLRTESLFETDLPYLAGAAPQPEFTL"
     gene            complement(15789..16454)
                     /locus_tag="PK818_02805"
     CDS             complement(15789..16454)
                     /locus_tag="PK818_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY81697.1"
                     /translation="MNQQVTSPPATAPAKPAGPGRPKDPGKRAAILDAAKRMFVSHGF
                     ERVSMDQIAAEAGVSKLTVYSHFGDKDRLFAEAVRAHCEQGMPTSLFVGEPGAPLRER
                     LTVIGSAFFSMIMTPEAIAGHRVLCSPQVATSAMPAMFWEAGPQRVQQAFAELLQRRV
                     ASGDLEIDDCARAASQFFTLLKGEPHAQAVFGYCCSGKADSPAQHVASVVDLFLRAYA
                     TRG"
     gene            16571..17641
                     /locus_tag="PK818_02810"
     CDS             16571..17641
                     /locus_tag="PK818_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY81698.1"
                     /translation="MALASLLAGCSEPDVPVEAARPVLVVHPSAGAQAGFSAYAGEIR
                     AREETALSFRIGGSLLRRLVDAGDHVRRGQLLAELDPGDAGLQVAAAQADLARLRGDL
                     ERYRTLVAQKLVSQSAFDAQQAAYRAARAQYDLTRNQSAYTRLLAPRDGVIANRQAEA
                     GQTVAAGQAVYTLAGDGGREVAIALPESRIREVSVGQPVLVELWSTPGQRLPGRVREI
                     SPAADPQARTYAARVALDGDAASAVELGQSARVYMQEHGEAAALRVPLSAVQRAADGR
                     PASLWVVDPKSRRVRLAAVKLGTYAEDVVPVLGGVDANAWVVAAGGHLLREGQLVAPV
                     DRDNRPVPGHSPTAMAPAAAVK"
     gene            17648..>18731
                     /locus_tag="PK818_02815"
     CDS             17648..>18731
                     /locus_tag="PK818_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY81699.1"
                     /translation="MHFNLSEWALRNRTLVLYALLMLGLLGAWSYRHLGQSEDPPFTF
                     KVMVVRTLWPGATAQEVSRQITDRIEKELMETGQYEYVRSYSRPGESQVIFMAKDSLR
                     SKDIPALWYQVRKKVGDIKGTLPRDAVGPFFNDEFGDTFGNIYALTGEGFDYAVLKDY
                     AERLELELQRVPDVGKVDLLGLQDEKIFIELSNTKLASLGIPLTTVQQALDEQNAVVP
                     ASYFETAGERVQMRVSGRFDSVQAIRDFPIRAGDRTFRLGEIATVTRGFSDPPAPRMR
                     FMGEDAIGLAVSMRAGGDILRLGKSLETEFARLQQTLPTGMQLRKVSDQPLAVTRSVD
                     EFIRVLAEAVAIVLLVSFLSLGLRTGT"
BASE COUNT         2561 a         6599 c         6736 g         2835 t
ORIGIN      
        1 cagatgttca gcgcgcgccc gctgctgggg cagggcaact atcgcggcgc gttcaggcag
       61 ctgtacctgt gcgggtccgg cacccacccg ggcggtggcg tcaccggcct gcccggccgc
      121 aatgccgcac gcgaaatcct caaggacctg ggcaaggggc cgcggccaga ctaacgccca
      181 gcgaaggtgc aggtggcggg caggggcggc gttcgcgtag cggcatttcg agccgcgcag
      241 cgaatgcctc ccctgcccac caccggtgat gcaccgacaa cggggcccag gtggcgccat
      301 caccaccggc aggcatgggt tggagccgcg cgggattcct gcactgagcg gtgctttttt
      361 gcactgcagc gtgcaaagcc cataacgatt ccttcacgat cctcgcgcgt cccgacgtgg
      421 gggagggagc aggcccgcag cgacttcggt cggtgcgggc ttttttgtgc ctgcgcctca
      481 gtccgccggc accgtgcgcg gcagcgccca tgcgcgcagg gcctgcacct gctcggccat
      541 cagcaccgac agcgggcggg tatcgcgcac cgcctgcagc agtgcatcgg tgtcgatgcc
      601 cgcctgcgcc gcatgcgcgg cgtacagcgc gctcacgatc acctgctcga tctcggcccc
      661 ggagtagccc tccgcggcgg ctgccagcgc gggcaggtcg atgccctcgc gcggcaggtt
      721 ccggcgcgca agatgcaggg cgaagatctc cgcgcgggcg gcggcatccg gcaggtcgat
      781 gaagaagatc tcgtcgaagc ggcccttgcg cagcagctcc ggcggcagtt catgcacctg
      841 gttggcggtg gcgaccagga acacccgcga cttgcgctcc gccatccagg tcagcaggta
      901 gcccagcacc cgccgcgaca gcccgccgtc gccctcgccg ctgccggcgg ccaggccctt
      961 ctcgatctcg tcgatccaca gcacgcacgg tgccagctgt tcggtggagg ccagcgcggc
     1021 acgcaggttc ttctccgact cgccgtggta cttgtcgtac agggtgccga aatccagccg
     1081 caccagcggc accccgaagc cgccggccac cgccttcgcc agcagcgact tgccgcagcc
     1141 ctgcacgccc agcaacagca cgcccttggg cgggtccagc ccgggcggtg gtggatcctg
     1201 caggaacggc agccggcgtt gttccaccca gcgcttgagc cgggccgcgc cgccaacatc
     1261 ggcgaagcgc gcggtgtcgt attcgtagtg caggtggccg ctcttgttga gcagttcgaa
     1321 cttcagccgc gccagttccg gcaggtcgcc catgcccagc gcaccatcgc gatagatcag
     1381 gtggcgggcg atccggcgcg catcggtcag gctcatgccc tgcaggttgc gcacgatctg
     1441 cttcactgct tcggcgtccg cttccacccg gcgcccgccg ttttcagcgg cataggcgcg
     1501 cgcctcgtcc tgcaccagtt tcagcagcgc attcgcatcc ggcaggcgca gcgcgaagcg
     1561 caccgcgaac ggctcaagcg cgggcggcag ttcgaccctg gtgccgatca tcaccagcac
     1621 gtgcggctgg cagtcgcggc gctggatcac gtcgcgcagc gcgcgctggt agctggcgta
     1681 gcccaggtag gggtgggcgt ccagcagcag gtaggtgccg cgctgggtgg cggaacggat
     1741 cgcctgcagg gtggcgctga ggtcgggcgc gccttcgctt tcgtcctcgc ggtcgatgtc
     1801 cagccggcgc aggccctcgg tcaccgacca gcggtgcagc gcgcgccagg cgtgcgtcag
     1861 cgactgccgg aacagcgcga tcacccgcga ttcgtccggg gtctcgatca ccagcaacgg
     1921 ggtttcggcg cggatcagcg ccagcaggtc ctgcagttcg ctcatggaga ggcacgatag
     1981 cggcgcggca ccgatgccac aatcgcggcc atggccgatc tcccgcagct cctcgaacgc
     2041 cgcctcgcgc aattgcccgc gaatgcgggc tggcgtgccg acgcagcatt catggtgaag
     2101 ctgcgcgcgc tcgccatcgc cagcgacttc gcgctggcca cgctggtcgc gcagccggcg
     2161 ctgctcgaac gcctgcacgc ggacgatggc ctcgcgcctt cgccgccgcc gctgctggag
     2221 gcgggcaacc gcgccgactg gccacgcctg ctgcggcgcc atcggcacgc cgaatccacc
     2281 cgcctggtgt ggcgcgacgt gattgccggg gatgcggtgg atgcgatcct ggccggcagc
     2341 accgcgctcg cggaaacctg cctgcaactg gcactgggtg cactggaaac cgaattcgcg
     2401 cagcgcttcg gcgtcctgcg cgatgccggg gggcaggcgc agcggctggt ggtgttcggg
     2461 cttggcaagc tcggtggcgg tgagctcaat ttcagttccg acatcgacct ggtctacgcc
     2521 tacgagcacg acggcgaatc ggatggcgcg cgcggcatca ccgcctccga ctatttcgcg
     2581 cggctcggcc agcaactggc gaagctgctg gacgaagtga ccgccgacgg cttctgccat
     2641 cgcgtcgacc tgcgcctgcg cccgttcggc aacgccggcc gggtggcgct gtcgttcgcg
     2701 gcgatggagc agtacttcca gcgcgagggc cgcgactggg agcgctacgc ctggcagaag
     2761 gcacggccgg tggccggcga cctcgccgcc ggcgagcgct tcctcgccgc gctgcgcccg
     2821 ttcgtgtacc gccgctacct ggacttcggc gcgctcgacg ggctgcgcac gatgaaggcg
     2881 gcgatcgccg ccgaggtggc gcgcaaggaa ctggccggcg acatcaagcg cgggcccggc
     2941 ggcatccgcg aaatcgagtt cctggtgcag gcgctgcaac tgatccatgg cggccgcgaa
     3001 cctgcactgc gcgaacgccg gctgttgccg gcgctgcagg cgctggtgca ggccgggcac
     3061 atggctgcgg atgcgggcgc gatgctggcg aacgcctatc gcgaactgcg cgtgctggag
     3121 aaccggatcc agatgctggc cgacgcgcaa acccatgcgc tgcccgccgt cccggtgcag
     3181 ggcgaacgca tcgcgcgtgg cctcggccac cccggcctgg cggcgctgga tgcccggttg
     3241 caggcggtgc gcacgcgcgt cgccgcggaa ttcgccgcgc tgctggcacc gcgcggggcg
     3301 gtggtcggcg acagcgacct gtcgctgtac tggcgcagcc tgcccgacga cggcgatgcc
     3361 ggtgcgctgg ccgccgccgg cttcggcgcc gccgaggagc tgcatcgcag cctgcgcgat
     3421 ttcgcgcggg cgcccggcgt gcgcgaactg tcggatgcca cccgtgcccg cctcgaccgg
     3481 gtgctgccgg ccctgctgca ggccgcgtcg aagtcggcgc aagcggatcc ggcgctgcgc
     3541 cggctgctgc cgctgctgca caccctgttg cgacgcacca gctacctggc cctgctggat
     3601 gagcaaccgg cggcgctgca gcgcctggtc gatgcgctgg cgcgcagcgc gctgctggcg
     3661 gaacggctgg gcgcgcatcc gctgctgctg gacgaactgc tcgaccgccg cgttgccggc
     3721 ggcctgccgg accgcgaggc gatgcaggca cagtgcgcgg cggcgttgcg cgagggcgat
     3781 gccgaagcct cgctgcatgc gctcaacgaa gtgcggcagg cgttgagctt ccggatcgcg
     3841 ctggcgttgc tggatgcgcg ctgcgatgcg caatcctgcg cgcaacggct ggcgtggctg
     3901 gcggatgcgg tggtcgcctg cgtgctgcgg atcgcgatgg gcgagatgcg cgcggcgcat
     3961 ggggtgattc ccgccgggcg tttcgcggtg ctcggctacg gcagcctggg cggcgaggaa
     4021 ctcggcttcg gttccgacct cgacctggtc ttcctgtacg actccgcgga cgacgcgcag
     4081 tccgatggtg cacggccact ggacgcgccg cgctggttcg cccggctcgg gcagaagatc
     4141 gtcgcgctgc tgggcgtggg caccggcgcc ggccgcctgt tcgaagtcga cgtgcgcctg
     4201 cgcccggatg gcgcgaaagg cttgctggtg tcgagcctcg ccagcttctg cgactaccag
     4261 cgcgaacgtg cctggacctg ggaacaccag gcgctggtgc gcgcgcgcgc ggtcgccggc
     4321 gatgccgaac tggtggcgga tttcgatgcg ctgcgcgcac aggtgctcgc gcgtccgcgc
     4381 gatccggcat cgctcgcgga ggatgtgtcg tcgatgcggc ggcggatgcg cgccgagctg
     4441 gatcgcagcg atgccgcggt gttcgacctc aagcagggcg agggcggcct ggtcgacctc
     4501 gaattcctgc tgcaattcct gctgctgcgg gatgccgccg agcacccggc cctgcgcgtg
     4561 ccgcgcgcga cgccggcctt gctggatgcg gttctggccg caggttcgat caccccggaa
     4621 acccacgcgt cactgctggc ggcgcacgcc agcctgctgg atgccggcat gcgctgcacc
     4681 ctggaccggc gcccgcggcg ggtaccgccg gatgcggcca tcgaggccgc ccgcgccgcg
     4741 atccgcgctg ccatttccgc gcaggggctg ggcgaaacgg ggtcagggtg attcttgcgc
     4801 cgatgcgagt cggcacggtc gcgatgttcg gtcgcgacac ttcacgctgt ccccgcttcg
     4861 cccagccgca gccgcacctc gcccgccacg cgcgcggtct cgcgcagcgg ttccgctgcg
     4921 aacggcgcca gcgtgtcgtc gaggtcgcgc acgcggccgc tgctgcgcaa tgcatcgagg
     4981 aaatgccgca ggcgtccgtt ggcgattcct gcgcaccaca tgaacaccgg cgccagcgtc
     5041 gccgcggctt cgctgagcat gttcaccgaa tccggcgtgc agacgatgcg gtcggcatgg
     5101 ccgagcagtc ctgcatacgg attcgtgcca tcgcggtcgt cgcgccagtg aaggccgggc
     5161 aatccggcgg gcatccccgc gagcatgctg cgccagtgcg ccggcgtgcg ccgcgaggcg
     5221 accacgctga aactcccatg caccgcgcgt gcatgcccgg cgagctgccg cagcagggtt
     5281 tcgaacgcag cgtcctgcag cgccacatgc cggctcgggc caccgaccag cagcacggta
     5341 tgcggtgaag gcagtgtggc gatgtgcgcg aagtcgcggc gcgcctgcgc cagccacagg
     5401 tcgtcgaccg ggtgcaggct gcccagtgtc cggatgacgt tgtcgccacg cagtgcgtcg
     5461 tgaccgggtg cgatcaccag gtcccagtgg cgcgggtcga tgcgcgggtc gaggatttgc
     5521 acggactggc tgccgcggtc gcgcagcagg cgggttgcca aagcggcctg gcggccacag
     5581 ccgatcgcga gcgccggcgg ctgcgcggcc agcgccttga aggcgttgcc gaacgcggca
     5641 tcggcgccgg gcagcacacg cggcgccagc catcgccacg gggcgcgcgg atcaagcgtg
     5701 caatcgccgg catccgaatc cagcgcgcgc gccagcgcca ccgcctggcg ggcgttgccg
     5761 gcgcggccat cgtgcacggc gaccgtcgtg ctcatgcgtt gcatgcccct ggctgttcca
     5821 ccggagtgat cgatccgttg cgattgcaat gctttggttt caccgatgga actctacact
     5881 tgtggcttgc ccgcaagctt ccgaggatcc catgaccgac gccctgaatg atgccgcgct
     5941 ggaccagctg ttccgcagcg cgcgcaccta caacgccttc accggcgaag tctccgacga
     6001 gaccctgcag cagctctacg gcctgctgaa gttcggcccg accgaggcca acaccacgcc
     6061 cgcgcggatc gtgttcgtca agtccgcgga agccaaggcc cggctggccc cggcactgag
     6121 cgagggcaac cgcgcgaaga cgctggcggc accggcggtg gcgatcatcg gctacgacat
     6181 gcgcttcttc gacaagctgc cggtgctgtt cccgcatgtc gacgccaagg cctggttcga
     6241 gcaccgcgac gaggccggcc tggcctgggt ggggctgcgc aacagcagcc tgcagaccgc
     6301 ctacctgatc ctggccgcgc gttcgctggg gctcgattgc gggccgatga ccggattcga
     6361 caacgcgatg gtcgacgccg cgttcttcgc cggcaccgcg gttcgctcca acatcctggt
     6421 caacctgggc cagggcgatc cggccagcct ctatccgcgt tcgccgcggc tggcgttcga
     6481 cgaagcctgc cagatcgcct gagcgcgcct gcccggtccc gattccacgc aaggagaacc
     6541 ccatgtccaa gcgtttcctg ctcaccgccg cgctggccgc ggccagtgcc aatgccttcg
     6601 cggccggcgt cacctacaag ctcgacccga cccacaccaa cgtcatcgcc agctggaacc
     6661 atttcggctt ttccaacccg agcgccaatt tcggcggcgt cgagggcacc ctggtctacg
     6721 acgccgccga cgtcgcgcgt tcaagcgtgc aggtgacctt gccgctgtcg ggcatggaca
     6781 gcttcgtgcc caagctcgac gagcacctgc gcggccccga tttcttcgac gccgcccgct
     6841 tcccggaagc ccgcttcagc agcacccggg tcgaggcggt ggccgccggc aagctgcgca
     6901 tcaccggcga cctgacgatc aggggcgtga cccggccgct ggtgctggac gccaccctga
     6961 acaaggccgg cgagcacccg atgctgaagg tggcggcgat cggcttcgat gccaccgcca
     7021 ccctcaagcg cagcgcgttc ggcctcggcg ggttcgcgcc cgcggtcagc gacgaagtca
     7081 ccttgcgcat caccaccgaa gcctcggcgt cggcgccggc cgccgcgggc aagtgaggcc
     7141 gcgatgatcg tcgagcgccc gtccgccgcg cgtggcgagg tcgacctggg ctggctgcgc
     7201 agccggcaca cgttctcctt cggccattac cacgacccgg cgtggatggg attcggcgcg
     7261 ctgcgggtga tcaacgacga caccgtcgcc cccggcggcg gcttcgcgcc gcaccgccac
     7321 gccaacatgg aaatcatcag cgtggtcctg gatggcgcgc tggcgcacaa ggacagcgcc
     7381 ggcaacgaag gcgtgatccg cgccggcgac gtgcagtgga tgagcgccgg ccatggcgtc
     7441 gagcacagcg agtacaacgg ctcgggcacc gaaccggtgc atttcctgca ggtctggatc
     7501 cagcccgacc ggctcaacgc gcagccggcc tacgcgcagc agtccttcga ccccgacgcg
     7561 cggcgtgcgc gctgggcgac gctgttttca gcggatggcg ccgacggatc gatcgcgatc
     7621 cgccagcagg ccagcctgcg cgccacccgc ctgcacgcgg gcgacgtggc gaccgcggca
     7681 ctggatccgg cgcgacgcta ctggctgcat gtcgcggccg gcgggatcga agcgggcgga
     7741 cgcgagctgc aggccggcga tgcgctgggc ttcgtcgatg aaagcggcgc actgccgctg
     7801 cgcgggatcg gcgatcgcag cgacgtgctg ctgttcgaac tgccgggctg agcgcatcga
     7861 cctgcgcagg cgtggggcaa gccgcgcgcg tgcagtaaac tggcgcggtt cccgcaaccg
     7921 cgccgcatcg ccatgaccca gcccgccccg tcgcctgcca aagccacgca gccccacccg
     7981 gtgcggcgcg cggacctgcc gctgagctgc ccgttgccgt cggcggcgtt gtggaatgcg
     8041 catccgcgcg tgttcctgcc gatcgaggcg gatggcggca gcagccagtg cccgtattgc
     8101 ggcgccgcct acgtgctggt ggactgacgc agcttcgcgt cgatgcgccg cctgaccgtg
     8161 gtgcaactgc tgcctgcgct tgaagcgggc ggcgtcgaac gctccaccct ggagatcgcc
     8221 gaagccctgg tgcgcgccgg ccatcgcgcc atcgtggtgt cggccggcgg ccggctggtg
     8281 gcgccgctgg aagcacgggg cgcccgccac gtcaccctcg acatcggccg caagtcgccc
     8341 gtcaccctgc gccacgtgcc ggccctgcgg cgattgttgc gcgaagccgg cgccgacatc
     8401 gtgcatgcgc gttcgcggct gccggcatgg cttggctggt tggcgctgcg cgggatgcgc
     8461 gcggcgcggc cgcatttcgt caccaccgcg cacggcctga attcgccctc gcgctacagc
     8521 gcgatcatgg cccgcggcga gcgcgtggtg tgcgtgtcgg aaacggtgcg cgactacctg
     8581 cagcagcact acccggcgac cgacccgcgg cggctggtgg tgatcccgcg cgggatcgac
     8641 cccgcggcgt tcccgcgcgc gccactgccg gatccggcgg cgcgcgcgcg gatcgccgcg
     8701 cggcacccgc agttgggcgg ccgtggcccg ctgctgctgc tgcccgggcg tggcacccgg
     8761 ctgaaggggc atgccgatgc gatcgcactg ctcgccgacc tgcgcggatc cgggcttgat
     8821 gcgcggctgt ggatgccggg cgcggtgcag gcggggcgcg cggcctatat ggccgaactg
     8881 gccgggctgg cgcgtgtcgc ccgcgtcgag gcggcgctgg cgatcaccga agcgaccgcc
     8941 gacatcgccc aggcctacgc cgccagcgac ctggtcctgc agctgtcgcg caagccggaa
     9001 gcgttcgggc gaaccgtgat cgaggcacac gcggtcggcc ggccggtgct gggctgggcc
     9061 catggcgggg tcggcgaact gttgcgcgcg ctgcaaccgc agggcgcggt ggcgccgttc
     9121 gaccaggccc tgctggcggc cactgcgcgc cgcctgctgg cgcagccgca accgccccca
     9181 gccggtacga tgcaggcctt ccagttgcac gcgatgcagg gggcgacctt ggcgctgtac
     9241 caatcgctgg tgggcgaatg agcgccacgg ccgcggcacc cggctggcgc tgggcaccgg
     9301 cgtgggtggt cgccttcgtc gccctgtggc ctgcgcccgg ggttgccgaa gcggtgctgg
     9361 cactgggtgc gatcgccgcc gtgctgcacc tgctgctgtc gcgcttccgc ggcagcgcgg
     9421 cactgctgac cggtccggcg tgggcactga cttcggtgct gttcttcgcc tactggctgc
     9481 cggaggcgat ttccgcgatc gacgccgccg accgcgcgcg cgcgctgcgc gaggccctgg
     9541 tcgacctgcg ctacctgccg ttcctgtggc tggtggcgtc ggcggtcacc ggcgcggagg
     9601 ggcggcggcg cacgttcggc gggctggcgg tgatcgttgc ggcatggacg ctggacgcac
     9661 tgctgcaggc cgccaccggc agcagcctgc tgttcgcggc catcgacggc ctcaagcagg
     9721 cgatcagcgg ccatggcatg tgcaccgcgg cggaggttgc agcacttgac cggctcagcg
     9781 gggtgctcgg gccgtgcaac ctcaagctcg gcatcgtgct ggcgagcctg tcgccgttcg
     9841 cgctggacgc ggcgggcagg cgctacggcc ggatcggctg ggcgctggcg gcggtgctgg
     9901 tcggactggc ggtgctgctg gcggggtcgc gcgcggcctg gctcagctat tgcctggtgc
     9961 tggcatggac cggctggcgc ctgctgggca tcaagcgcct gcttgcggtc ttcgcgcttg
    10021 gcatcgccct gctgctggcc tcggccctgg tctcgccgcc ggttgccgaa cgcatcaacc
    10081 gcagcaccgc ggtgttcgtc ggcaatgcag agggcgtgga ccatgcgctg tccggccgca
    10141 gccggatctg gtcggcggcg ctatgcatgg cgcgcacgca tccgatcaac ggggtcggtg
    10201 cgcgcggctt ccgcgaagcc ttcccggcct gcgacaccga acccggcacc cgcgcggcat
    10261 ggggtgacgg gccggccctg catgcacacc aggtcgtgct ggaggtgctc agcgaaaccg
    10321 gcggcatcgg cctgctgctg tggctggccg gggcggcgat ggcctggcgc gcgtggcgct
    10381 acgcggacgc cgcggcccgc gaccagtcgc gcccggcgat gctggcgctg gcggtcaccg
    10441 tgtttccgct gaacacgcac ctggcggcct attcgacatt ctggggcggc gtcctgctgc
    10501 tgctggcggc gctgtacgcc ggcagcctgc tggggcgcga accggcatga ggtgccggct
    10561 ctccggcgtg gtcaccacgc tggacaatgc gtccacgctg gaggactgcc tggccagcct
    10621 ggcgttctgc gacgagatcg tcgtgctgga ttcgggctcc agcgacgcca ccctcgcgat
    10681 cgccgcacgc cacggcgcgc gcaccgcaag ccaggccttc gccggctacg ggccgcagaa
    10741 acaggcggcg atcgggcttg cgtccggtga ctggatcctg ctgcttgacg ccgacgaaca
    10801 cctgaccgct gccggccgca gcgcgatcga acgcgaactt gccgcgccgc gcgcggacgg
    10861 ctaccggctg ccgcgccggg aatggctgtt ctggcgctgg ccgcactcgg ccacccgcgc
    10921 gaactggcaa ctgcgcctgt tccgcaaggc gacagggcgg atgaacgatg ttcccgtgca
    10981 tgccgcgccg gaggtggcgg gccgggtgct tgaccttgat gcgccgttcc gccattacgg
    11041 cgaaccacgc ctggccgacc gcgtcgacaa ggtcaaccgc tattcgtccg ggctggtcga
    11101 acacaagcgc ggccggcgcc cgcgtttcat cgccctgcgc ctgctgttct acccgggcgc
    11161 ggcgttcctc aagctctacc tcggcaagcg ctatttcctc aacggctggg ccggtttctt
    11221 cgcggcgaag acgcagtcgt tctatgcctt cctcaagtac gccaaggtgc tggaagcagc
    11281 gcgtcaggac gcttcgcgcg agtagcggga cacgccgtcc ggctgccgct tgaagcgccg
    11341 gtgcagccac aggtactggc tgggcgcctg gcgcaccatc gcctcgatcg cggcgttgac
    11401 cgcgctgccg tccgccgccg catcgtcggt ggggaacggc gccagcggtt cgccgatgcg
    11461 cagcaggtag cggtcgcctc catccttgcc ggggatgcgc cgatggaaga acggcaccac
    11521 cgcgcagccg gtgagccgcg cgaactggtg ggtggcggtg atggtcgccg ccgggatgcc
    11581 gaagaacggc gcgaacacgg tgtcctttcc gcgcatgtcc tggtcgggcg cgtaccacag
    11641 cagtccgccg cgcttgaggt ggcggatcgc ggcgcgggtg tcgccgttgc cgaacatcgc
    11701 gctggcgtag cccagccggc cgcgcagcac cgcccactcc atcaccgggc tgcgatggcg
    11761 gcggtacatg ccggccagcg acacgtggtc gcacagcagg cggccgcaca tctccagggt
    11821 catgaaatgc ccggacacaa gcagcacgcc gcggccttct gcctgcaggc ggccgaggat
    11881 gtcgaggccc tcgatccgcg cggtttgccg catcggcgcc gcgtcgcccc accaggcgcg
    11941 ggcgaactcg aacaggccga tgccgacgtc gcggaagttc tcccgcaata gccgttcgcg
    12001 ttcggacgtc gacagctccg gcatgcacag tgccaggttg gtgcgcgcgg cgcggcggcg
    12061 gctcggcatg agccgcaggg ccagccagcc cacggccgca cccatcgccc gctgcagcgg
    12121 ccacggcagg cgcgccaggc ccatgcaaac cgccacgccg aaccacaccg gccaatggcg
    12181 caggccgcgc ggcgggatcg gcaggtcggg cggggcggcg gcggacatgc cacggattct
    12241 aaccagcgcc tgcggatcgg gcgggccggc ttcgttatgc tgtgccgatg cgggccgacc
    12301 tcaccgagcg cgtgctgcgc ggcctgtatt ccgccgccct gtacatcctt gcgccaatca
    12361 ccctgtacca cctgatctgg cgcgggttcc gccagcgtgc ctacttccag cgctggaacg
    12421 agcgctacgc cagctacccg gcggtcaccg cggccacccc gctgtggctg catgcggtgt
    12481 cggtgggcga ggtcaatgcc gccgcaccgc tggtcacccg gctgctgcag gaccatcccg
    12541 gcctgcgcct ggtggtcacc accatcaccc cgaccggatc ggagcgggtg caggcgctgt
    12601 ggggcgatgc ggtggtccat gtctacctgc cctacgactt gccgggcgcg gtggcgcgct
    12661 tcctcgacca cttccggccg gcactggcgc tgatcgtgga aaccgagttg tggcccaacc
    12721 tgctgttcgc ctgccgcgat cgcggcatcc ccacccacat cctcaatgcg cggctgtcgg
    12781 cgcgctcgct gcgcggctat cgcgtgctcg gtccgctgat ccggcgcgcg ctgcgcggcg
    12841 tgcacctggt cgccgcgcag tccgacgagg acggcaagcg cttcatccgg cttggcgcgg
    12901 atcccgggcg ggtggtggtg accggcaacc tcaagttcga caccctgccg gtggacaaca
    12961 ccgatttcgt gcgtgaattc cgccaccatc gcggcagcgg cccgacctgg atcgccgcca
    13021 gcacccacga ggacgaggag gcggcggtgc tcgcggccca ccacgcggtg tcgcaagcgc
    13081 atccggatgc gttgctggtg tgggcgccac ggcacccgga gcgcttccgc gcggtcgccc
    13141 agcgcgcggc ggaggccggc ttcgcggtgg ccacccgcag cgagcagggc tggccgcgga
    13201 acgacgcgcg cggacgcatc caggtgttcg tggtcgatac cctgggcgaa ctgacgcgct
    13261 tctatgcctg cgcgcaggtg gcattcgtcg gcggcagcct gcagcccgtc ggcgggcaca
    13321 acctgctgga acccgcggcc accggcaccg cgatcctcag cggcccgcag ctgcacaact
    13381 tcgtcgatat cgccaggcgc ctgcgcgagg ccgaggcgat gcgcatcgtc gaggatgcgc
    13441 ccgcactggc gtcggcgttg ctggagctgt tcgccgacgc cgatgcgcgc gaacgcatgg
    13501 ccgcgaacgc gcagcagttg ctgcaggccg ggcgcggcgc gctggagcgc accgtggccc
    13561 tgatcgaacc cgggttgccg cggcgcggct agtccccggt cagggcgtcg cggtggcgac
    13621 acccaccgcc ttgtcggtgg tgagcaggcg gttcacgtcc tcgacgtcgg agacgcccag
    13681 ggtgccggcg gcctgttcca gcaacaggcg gttctgcagg aagttgtact tcacccgcgc
    13741 atagacctgc tgcgcggtga acagcgtgcg ctggttgtcc agcacgtcca gcacggtgcg
    13801 ggtgccgact tccaggccga cctgcgacgc ctcgtaggcg ctttgcgccg agaacagcgc
    13861 cagccgccgg gcctcgacct cgctgatgcc cgccaccagg gtctggtagg catcgcgggt
    13921 gctgcgttcg atcgcgcgcc gggtctgttc caccgcgtcc tgggtggcat cgcgctgggc
    13981 cagcgcggag cgcacgccgg actgggtggc gaggcccgag aagatcggca cgctcagggt
    14041 cagcccgacg ttggtcgcgg ccttgttggc gccgacgtag tccggatcgt acagccccgg
    14101 gatcgccttg ccgtaggagg cgcgcaggcc cagggtcggg tagtggccgg agcgggcggt
    14161 ttcgacattg gcttcggcgg cctcgaggtc ggcggcctgc gccttcagcg aggggttttc
    14221 ggccaacgcg gtggccaccc agccctcgac atcgcgcgct tccggcagtg ccggcttgaa
    14281 atcgtccggc aggcccttga ggttggcgac cggggtgccg gtgatctcga ccagcgcctg
    14341 gtaggcgtcc tgcaccgcat tgcgggcgag gatggtgttg gcgcgcgcgc cgtcgtactg
    14401 ggcgcgcgct tcatgcacgt cggtgatcgg cgccaggccg acctcgaggc gcttcgacgc
    14461 gaagtcgaac tgcttcttca gcgcggcctc ggcggcttcg gaggcagcca gcgattccag
    14521 ctggatcagc acgttgaagt aggccgccga ggtgcgggtg atcaggttat ggcccgctgc
    14581 atcgaggtcg aaggcgcccg ccctgtcctg cgccttggcg gcgcgcagcg cggtgtagtt
    14641 gccgtaatcg aacaacgact gcgacatgtc cacgccccag gagccggtgc gtgcgctgtt
    14701 gccgccctgg ttgacgcggc tcacgccaac cgaaccgccg acttgcggca gcaacgcggc
    14761 gcgagcctgg atcggccgtt ccgcggcgac caggcggctg gattcggcgg cggcgaactg
    14821 cggatcgctg ttgcgtgcgc tgttgtagct ctgcagcagg tcgtcggcct gagccgcggc
    14881 gggcatcagg gcgagggcaa gggcgaaggc gagcgggcga cgcagcatgg ggggtccttt
    14941 ggcgcgaatc attcgggggc gtgggcggtc tggaacgcgg caggtgccta cagcgtgaat
    15001 tccggttgcg gcgcggcacc ggccaggtac ggcaggtcgg tctcgaacaa cgattcggtg
    15061 cgcaggccgt tgacgtcgcg atgcaccagg gtggcctgca tcgccggcga acgcccctgc
    15121 accacgaaca tgcggccacc gggcttcagc cattcgacga agcgcggcgg cagcgcgtgc
    15181 accgcgccgc tgacgcagat cgcgtcgaag tgctggtcgt tgtcccaggc cagcgcatcg
    15241 gcggtctcga cccgggcatt gccgcaaccc tgcgccgcca gccgcgcgcg cgcggcgtcg
    15301 gcgaggtcgg cgtgcagttc gatcgccacc acttcgcggg cgaggcgggc aaggcaggcg
    15361 gtgaggtagc cgctgccggc gccgatctcg agcacccgtt cctcggcgcc ggggcgcagc
    15421 gcctgcaggg cgcggccttc gagtaccggt ttcatcatcg attcgccatg gcccagcggc
    15481 aacgctgtat cggcataggc cagcgcacgc accgcttccg ggacgaaggc ctcgcgcggg
    15541 acgctgccga tggtctccag cacccggggg tcgaggacgt cccacgggcg gacctgctgt
    15601 tccaccatgg tttcgcgggc cttggcgtag tcgatgctca tgcgcttgga atccgttgcg
    15661 gggaaccggt cattgtagcg gtcacgacct gcccgatgcg tggcagtttc gctgtgctag
    15721 cacagcggca cgcagtgccg gaagtcacca gtaccggtgc cacccgggcg gccgccgagg
    15781 cgcggcggtc agccgcgggt cgcatacgcg cgcaggaaca ggtcgaccac cgacgccacg
    15841 tgctgcgccg ggctgtcggc cttgccgctg cagcaatagc cgaacacggc ctgtgcgtgg
    15901 ggttcgccct tcagcagggt gaagaactgc gatgccgcgc gcgcgcaatc atcgatctcg
    15961 aggtcgccac tggcgacccg gcgctgcagc agttccgcga acgcctgctg cacgcgctgc
    16021 gggccggcct cccagaacat cgccggcatc gcgctggtcg cgacctgcgg ggagcacagg
    16081 acccgatgcc cggcgatcgc ctccggggtc atgatcatgc tgaagaaggc gctgccgatg
    16141 acggtcaggc gttcgcgcag cggtgcaccc ggctcgccca cgaacaggct ggtcggcatg
    16201 ccctgttcgc aatgcgcgcg caccgcctcc gcgaacaggc ggtccttgtc gccgaaatgg
    16261 ctgtagacgg tgagcttgga gaccccggcc tcggccgcga tctggtccat gctgacccgt
    16321 tcgaaaccgt ggctgacgaa catccgcttg gccgcgtcca ggatcgccgc gcgcttgccc
    16381 gggtccttcg ggcggccggg gccggccggc ttggcgggag cggtggcggg cggcgacgtg
    16441 acctgttgat tcatgcttga atactagact agttggttcg atatttatac tataccgcca
    16501 agtttattaa tcttggcgaa ccgccggcgg gggcgagatg gggcacacga cgaagattcc
    16561 gatgttggcg gtggcgctgg cgtccctgct ggcggggtgc agcgagccgg atgtcccggt
    16621 cgaggccgca cgcccggtgc tggtggtgca tccgtccgct ggcgcgcagg cgggattcag
    16681 cgcctacgcc ggcgaaatcc gcgcccgcga ggaaaccgcg ttgtcgttcc gcatcggcgg
    16741 cagcctgttg cgacggctgg tggatgccgg cgaccacgtg cgccgcggcc agttgctggc
    16801 cgaactcgac cccggcgatg ccggcctgca ggtcgccgcc gcgcaggcgg acctggcgcg
    16861 cctgcgcggc gacctcgaac gctaccgcac gctggtcgcg cagaagctgg tcagccagtc
    16921 ggcgttcgat gcgcagcagg cggcctaccg cgcggcccgc gcgcagtacg acctgacccg
    16981 caaccagtcc gcctacaccc gcctgctggc gccgcgtgat ggcgtgatcg ccaatcgcca
    17041 ggccgaggcc gggcagacgg tggcggcagg gcaggcggtg tacaccctcg ccggcgacgg
    17101 cgggcgcgaa gtcgcgatcg cgctgccgga gtcgcgcatc cgcgaggtca gcgtgggcca
    17161 gccggtgctg gtcgaactgt ggagcacgcc gggccagcgc ctgccgggcc gcgtgcgcga
    17221 gatttccccc gccgccgatc cgcaggcgcg cacctatgcc gcgcgcgtcg cgctggatgg
    17281 cgatgccgcc agcgcggtcg agctgggcca gagcgcgcgc gtctacatgc aggagcatgg
    17341 cgaagccgcg gcattgcgcg tgccgctgtc ggcggtgcag cgtgccgccg atggcaggcc
    17401 cgcctcgttg tgggtggtcg atccgaagtc gcggcgggtg cgcctcgctg cggtgaagct
    17461 gggcacctac gccgaagacg tggtgccggt gctgggcggc gtcgatgcca atgcgtgggt
    17521 cgtcgccgcc ggcggccacc tgctgcgcga gggccagctg gtcgcgccgg tggatcgcga
    17581 caaccgcccc gtgcccgggc attccccgac agcgatggcc cccgcggccg cggtgaagta
    17641 ggccgccatg catttcaacc tctccgaatg ggcgctgcgc aatcgcaccc tggtgctgta
    17701 cgcgctgctg atgctgggcc tgctcggcgc ctggtcctac cgccacctcg gccagtccga
    17761 ggatccgccg ttcacgttca aggtcatggt ggtgcgcacc ctgtggccgg gcgcgaccgc
    17821 gcaggaagtg tcgcggcaga tcaccgaccg catcgaaaag gagttgatgg agaccgggca
    17881 gtacgagtac gtgcgctcgt actcgcggcc aggagaatcg caggtcatct tcatggccaa
    17941 ggattcgctg cgcagcaagg acatcccggc gctgtggtac caggtgcgca agaaggtcgg
    18001 cgacatcaag ggaaccctgc cgcgcgatgc cgtcggcccg ttcttcaacg acgagttcgg
    18061 cgacaccttc ggcaacatct acgcgctgac cggcgagggc ttcgactacg ccgtgctcaa
    18121 ggactacgcc gagcggctgg agctggaact gcagcgcgtc cccgacgtcg gcaaggtcga
    18181 cctgctcggc ctgcaggacg agaagatctt catcgaattg tccaacacca agctggcctc
    18241 gctcggcatc ccgctgacca cggtgcagca ggcgctggac gaacagaacg cggtggtgcc
    18301 ggccagctat ttcgaaaccg ccggcgagcg cgtgcagatg cgcgtcagcg gccgtttcga
    18361 ttcggtgcag gcgatccgcg atttcccgat ccgcgccggc gaccgcacct tccgcctcgg
    18421 cgagatcgcc accgtcaccc gcggcttctc cgacccgccg gcgccgcgca tgcgcttcat
    18481 gggcgaggac gcgatcgggc tcgcggtgtc gatgcgcgcc ggcggcgaca tcctcaggct
    18541 cggcaagtcg ctggaaaccg agttcgcgcg cctgcagcag accctgccga ccggcatgca
    18601 gctgcgaaag gtctccgacc agccgctggc ggtgacccgc tcggtcgacg aattcatccg
    18661 ggtgctggcg gaggccgtcg ccatcgtgct gctggtcagc ttcctgtcgc tgggcctgcg
    18721 caccggcacc g
//
LOCUS       DAORUK010000023        18371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_2679_length_18371_cov_19.295152, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000023 DAORUK010000000
VERSION     DAORUK010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 18371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18371
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2679_length_18371_cov_19.295152"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /gene="leuB"
                     /locus_tag="PK818_02820"
     CDS             complement(<1..739)
                     /gene="leuB"
                     /locus_tag="PK818_02820"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009606966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HQY81700.1"
                     /translation="MHANILVLPGDGIGTEVTAAAVSVLRAVARRYGHEFHFEERLIG
                     GAAIDATGEPLPAATLDAARKADAVLLGAVGGPRWSDPKAKVRPEQGLLAIRKALGLY
                     ANLRPVKPHRATLGASPVKPHLLNGVDLVVVRELTGGIYFGDKTRDAGAASDLCSYSV
                     AEIERVVRHACTLARARRGKLTSVDKANVLETSRLWRETTERLAREEFPDVTLEHQLV
                     DSMAMHLLAKPREYDVIVTENMFGDILT"
     gene            complement(739..>1062)
                     /locus_tag="PK818_02825"
     CDS             complement(739..>1062)
                     /locus_tag="PK818_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HQY81701.1"
                     /translation="LLDLGLRAVISSQIADIFRSNALKNGLLPVVLDEAIVEELLAQP
                     GIELAIDIATRTLALPDGRRFGFPLDAFAQACLLEGVDQLGYLLTRHPAIQRFEAARD
                     AAGAA"
     assembly_gap    1063..1162
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1200..2612)
                     /gene="leuC"
                     /locus_tag="PK818_02830"
     CDS             complement(1200..2612)
                     /gene="leuC"
                     /locus_tag="PK818_02830"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HQY81702.1"
                     /translation="MPPRTLFDKLWDAHLVSPETDAAPAVLYVDLHLIHEVTSPQAFT
                     ELATRGLPVARPERTKGTLDHSTPTLPADAAGKLPYANPEAEAQVAKLRENAAAFGVE
                     LFDFGSAHRGIVHVIGPELGITQPGMTIVCGDSHTATHGAFGALAFGIGTSEVGHVLA
                     TQCLLQRKPKTLAITIDGALGDGVSAKDLILHVIGVIGVNGGTGHVIEYRGSTIRAMS
                     MDERMTVCNMSIEAGARAGLIAPDHTTFEFLRHTPRAPRGEAFERAVANWREFRSDDG
                     ASFDREVHVDAADIAPTITWGTHPGQVAAIAACIPADENSDDAQARDYMGWDPQSPLA
                     GRAVDVVFVGSCTNSRLSDLRAAANVLRGRKVHPRVRMLVVPGSEQVKKDAEAEGIDA
                     IVRAAGGEWREPGCSMCIAMNGDLVQPGQLAVSTSNRNFEGRQGKGARTVLASPLTAA
                     VCAVTGEITDPRLFLHREAA"
     gene            complement(2612..4171)
                     /locus_tag="PK818_02835"
     CDS             complement(2612..4171)
                     /locus_tag="PK818_02835"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HQY81703.1"
                     /translation="MNRSATTDRTQPHRIRIFDTTLRDGEQSPGFTMSAPQKIAFAHA
                     LEDLGVDVIEAGFPNSSPADFGAVQAIAREVRGASIAALARCHPADIEACARALDGAA
                     APRIHAFISTSPLHRQHKLNMSRGQVIEHAVMGVELARRHVDDVEFSAEDAFRTEREF
                     LVEIFDAAIQAGARTLNVPDTVGYATPAEIRALFEYLRANVKGADGVVFSAHCHDDLG
                     LAVANSLAAIEGGARQVECTINGIGERAGNAALEELAMAMKVRGGWYGADTGIDTRKL
                     LQTSRLLSRITGIQVQRNKAIVGLNAFAHESGIHQHGMLKNRDTYEIMRPQDVGWADS
                     QLVLGRHSGRAAVADRLRQLGYVLEDEQLDQVIAGAKALTQTQHVINDGDLQRLVEGE
                     QHGPGWRISALSLTDYGQRTRAHVDLSDPDGRHVMQSAEGEGPVAALFAALAQATGVD
                     FVLESYEVHSMGIGRDARGEANLGGRIDGEAMAGQGTSRDVLEASAIAWLDVANRVLR
                     MRLAPPRVEAA"
     gene            complement(4336..5313)
                     /locus_tag="PK818_02840"
                     /pseudo
     CDS             complement(4336..5313)
                     /locus_tag="PK818_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
     gene            complement(5318..6349)
                     /locus_tag="PK818_02845"
     CDS             complement(5318..6349)
                     /locus_tag="PK818_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HQY81704.1"
                     /translation="MQLNDGQAAESRGEGARSDQSTPPRPARPTGTADVRSPAREPMV
                     RERAASPPAAQGRESRLWFDGELHDATKLQATLGTHALHYGSGVFEGIRAYATRDGAA
                     VFRLPEHLERMRKGCELLGMDFDVAEATAAVLQTLRANRLGDAYIRPLAWYGDGGFGL
                     DVTGRSHHLMVATSATNLHLNGNRTRVSVSQWRRNPASSLPPLKLCGGYVNSILAKRE
                     VRLRGYDEAMFVDDDGYAVECTGANLFLVRDGGITAVEHRDALPGITRDTLVRITGAD
                     SRPVSMEELLDADEVFACGTAAEVAPIAEIDGRRFDANSPLTREIAAVYAATVRGESS
                     WSGRWLTKA"
     gene            complement(6340..6576)
                     /locus_tag="PK818_02850"
     CDS             complement(6340..6576)
                     /locus_tag="PK818_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HQY81705.1"
                     /translation="MRYRLDLTLRHAEGALARVLGTTERRGFRPVAVEGETLTDGDRW
                     HLQMTVEGERPAETLHAQLAKLYDCLHVQVQPCS"
     gene            complement(6578..8326)
                     /gene="ilvG"
                     /locus_tag="PK818_02855"
     CDS             complement(6578..8326)
                     /gene="ilvG"
                     /locus_tag="PK818_02855"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HQY81706.1"
                     /translation="MNGARWLAQALVAEGVDTLFGYPGGTIMPFYDALHGTPALKHVL
                     VRHEQGAAFAANGYARASGRVGVCVATSGPGASNLVTGIADAMLDSVPMVVITGQVPT
                     TLMGTDAFQELDVFAMTLPMVKHSFIARRVEDLPMMVAEAFRLARSGRPGPVLIDLPK
                     DVQMAAAAHLPAHAPLPVDAAAAPKDASLHEALALISQAQRPVLYGGGGIALGDAVQD
                     FRTFVDATQIPTVLTLKGLGALPTRHPLNLGMLGMHGSRAANLAVQESDLLVVVGARF
                     DDRATGKLAEFAPHARVVHMDLEACEIGKLRCADAGVRGDIRTTLARLTLPCAAHLHG
                     RNGQARKAWRAQCRQRAQQHAARYDAPGDSVFAPALLKRLSELAPDAVVACDVGQHQM
                     WVAQHWRLDHPRKHLTSGALGAMGFGLPAAIGAQLQDPAARVVCVSGDGSIQMNIQEL
                     ATLRRYQLPVKIVLLDNQALGMVRQWQELFFEKRYSEVDLSDNPDFVALARAFGIQAL
                     HLDRADGMDAALQALLAAEGPALLHVAIDTAANVWPLVPPNRSNSEMLEDAATEAAPA
                     IRASNPLPESTSVQEA"
     gene            complement(8326..9351)
                     /gene="ilvC"
                     /locus_tag="PK818_02860"
     CDS             complement(8326..9351)
                     /gene="ilvC"
                     /locus_tag="PK818_02860"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HQY81707.1"
                     /translation="MTSTTAKPKVTIVGYGSQGRAHALNLRDSGFDVTIGLRPGGPTE
                     IKARADGFTVKAPAEAVKDAGIVAILTPDMVQPQLYGEVIEPNIAAGACLLFAHGFNV
                     HYQQIAPRDDLDVVLVAPKGPGALVRREYEIGRGVPAVYAVHQDRSGNAEQLALAYCA
                     GIGGARQNAIRTTFKEETETDLFGEQAVLCGGATKLVQAGWETLVEAGYQPEVAYYEC
                     LHELKLIVDLFYEGGITRMHEFISETAQYGALTRGDYIVDAHTRAQMKKVLTEIQDGT
                     FARQWIAEYAAGNANYRAMKQADLEHPVEAIGKKLRANMKWLASSQPAAAKPATASET
                     PPQSEAA"
     gene            complement(9394..11139)
                     /locus_tag="PK818_02865"
     CDS             complement(9394..11139)
                     /locus_tag="PK818_02865"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQY81708.1"
                     /translation="MRSNAIKQGPTRSPARAMLRATGLDDAAIARPFVAVVHTWSDVS
                     PCNINLRDLAQHVRRGIDAAGGTPIEFNTIAVTDGIAMGSDGMRASLPSRETIADSIE
                     LAVGGHCLDAMVLLVGCDKTIPAAAMAAARLDIPTVILYGGTILPGHCPTTDGEAPKP
                     LTVQDVFEAVGAHAAGRIDDAGLHAIESHACPGAGACGGQFTANTMAMVLTFLGLSPL
                     GANDIPATDPAKADAGYACGELAMALLRDGGPTPRTLLTTRALRNAARAVSATAGSTN
                     AVLHLLAIAHEAGVAFDLEEFEAASASTPVIADLKPGGRYTAAEMHAQGGTALVAREL
                     QAAGLIEDIPTVAGRSFFAQADAAQTPPAQDVVRPVSDPIKPRGGYSILYGDLAPEGC
                     IVKLAGHGIERFEGRARVFDSEEAAFAAVQARAIVAGDVVVIRFEGPAGGPGMREMLA
                     VTAALVGQGLSNDVALVTDGRFSGATHGFMVGHVAPEAARGGPIAKLRDGDRVRIDVA
                     SRRIDVAADLSQRTPARMAPRVRCGALAKFARDVGSASRGAVTSPGPLDAHDDDARRI
                     ASSLLDAATDAAIPA"
     gene            complement(11250..11426)
                     /locus_tag="PK818_02870"
     CDS             complement(11250..11426)
                     /locus_tag="PK818_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81709.1"
                     /translation="MTQRNIHPQATRTPSQDAVAAGPRALPLSFAHVALAATLVLVLV
                     LIISGQSGAGVGFV"
     gene            11628..13487
                     /locus_tag="PK818_02875"
     CDS             11628..13487
                     /locus_tag="PK818_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY81710.1"
                     /translation="MPAARVAVKPTDIRNPDVFHKVVDCQWACPAHTPVPEYIRLIAA
                     GRHADAYMVNWRSNVFPGILGRTCDRPCEPACRRGRVEHNNGPGAMRDNPEPVAICRL
                     KRVAADHKGDVRARMPKPASQRNGKRVACVGAGPSSLTVARDLAPLGYQVTLFEADPK
                     AGGFMRTQVPRFRLPEQVIDEECGYILDLGVELVAGRRIESMRALLAEGYDAVFVGSG
                     APRGRDLRIPGRDEAAANIHLGLDWLANVYFGHVAAVGKRVLVLGGGNTAMDCCRSAR
                     RLGGEDVKVLVRSGFDEMKASPWEKEDAIHEGIPILDCRVPGAFVHEGGKLTGMTFQR
                     VRPEFDGNGRRMLVPTGEPDEFHACDEVLIAIGQENAFPWIERDCGIAFGEWDMPVVD
                     PLTHQSSLPHVLFGGDAAFGPKNIIWAVAHGHAAATSIDRLLHGEDPRERPDPLVRLT
                     SQKMGIHEWSYDNGVSADQRFAVPWSEEARKLDSIKVEVELGFDAATALKETQRCLNC
                     DVQTVFTRDKCIECDACVDICPTDCITFTGNGEEAALRTRLTAPALNAAQDLYVSEPL
                     PTMRVMVKDEDVCLHCGLCAERCPTGAWDMQKFLLLETHAGTRCGGSPAREAA"
     gene            13484..15331
                     /locus_tag="PK818_02880"
     CDS             13484..15331
                     /locus_tag="PK818_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HQY81711.1"
                     /translation="MSMAIDPPAVAALQAVNDFVVKFANVNGSGSASANELFAKSILR
                     MGVPVSPRNIFPSNIQGLPTWYEVRVSEAGWLGRRGGVDMLVAMNPQTWDDDVRELAP
                     GGYLFYDSSKPLPKSKFRDDITVIGVPLTELCNAAYQDPRQRQLFKNIMYVGALGALL
                     DIDMAAIEALVGEQYRTKPKLLAPNIDALHMGRGWVHEHLPHPIGLRVRKADAVGDRI
                     FVEGNAAAALGCVYGGATVCAWYPITPSSSVAEAFQRNCQKFRIDDAGRARFAVIQAE
                     DEIASIGMVVGAGWNGARAFTSTSGPGISLMNEFIGLAYFAEIPATIINVQRGGPSTG
                     MPTRTQQSDVLACAHASHGDTKHVLLFPEDPAECFGFAAEALDLADRLQTPVFVMSDL
                     DIGMNQRLCAPFRWDDARRYDRGKVMTAEELEAGREFARYKDVDGDGIPYRTLPGTHP
                     DKGAYFTRGTSRDPQAKYSERGADYLYNMQRLLHKFETAKALVPQPVVDQSVPPAPQA
                     AIFYGSTSPAMAEAVELLRDAGIALDSVRIRAFPFADSVRDFIAAHESVFVVEQNRDG
                     QLRSLLVDEFGIDPARLVAVLHYDGTPITARFIAGAIEARLRPARTEVA"
     gene            15335..16390
                     /locus_tag="PK818_02885"
     CDS             15335..16390
                     /locus_tag="PK818_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HQY81712.1"
                     /translation="MTYLAKPRLHHPSLRCNALGFTRRDYEGTVSTLCAGCGHDSISA
                     AIIQACWEMDIAPHRVAKLSGIGCSSKTPTYFLGQSHGFNTVHGRMPSVLTGANLANR
                     GLIYLGVSGDGDSASIGIGQFVHSIRRAVDMTYIVENNGVYGLTKGQFSATADQGSVS
                     KKGVANNDSPLDLVTMALQLGATYVARSFSGDKQQLVPLIKGALAHRGAAFIDVVSPC
                     IAFNNHPGSTKSYEYVREHNEAVNRLDVVLPRAEISAEYAEGELLEIDQHDGSVLRLR
                     KLDADYDPRDRIRAMNLLQQRQAQGEVVTGLLYVAADAEDLHRHLNTIETPLNALGAE
                     ELNPGAEALERINASLR"
     regulatory      16444..16547
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16620..>18371
                     /gene="thiC"
                     /locus_tag="PK818_02890"
     CDS             16620..>18371
                     /gene="thiC"
                     /locus_tag="PK818_02890"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HQY81713.1"
                     /translation="MNAVPSELIRQAEQLSEAVIRPIPGSRKVHVAGSRADLRVPMRE
                     IELARTPTLFGGEDNPPLAVYDCSGPYTDPAVRIDLAAGLPALRAQWIAERGDTDVLE
                     RISSEFGRAREVDPKLAAVRFPGRSLPRRARAGANVSQMHYARRGIVTPEMEFVAIRE
                     NQRIEAVRDAILLRQHPGEGFGASIQKFITPEFVRDEIARGRAILPCNINHPESEPMI
                     IGRNFLTKINANIGNSAVSSGIAEEVEKLVWAIRWGADNVMDLSTGKHIHETREWIVR
                     NSPVPIGTVPIYQALEKVDGRAEELTWEIFRDTLVEQAEQGVDYFTIHAGVLLRYVPL
                     TAKRVTGIVSRGGSILAKWCLAHHRENFLYTHFEDICEIMKAYDVAFSLGDGLRPGCI
                     ADANDAAQFGELETLGELTKVAWKHDVQTMIEGPGHVPMQLIKENMDKQLAECGEAPF
                     YTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNRQDVRDGIMAY
                     KIAAHAADLAKGHPGAQVRDNALSKARFEFRWQDQFNLGLDPEKAREFHDETLPKDAH
                     KVAHFCSMCGPHFCSMKI"
BASE COUNT         2668 a         6319 c         6476 g         2808 t
ORIGIN      
        1 cggtgaggat gtcgccgaac atgttctcgg tgacgatcac gtcgtactcg cgcggcttcg
       61 ccagcaggtg catcgccatc gaatcgacca gctggtgttc cagggtgacg tcggggaatt
      121 cctcccgcgc caggcgctcg gtggtttcgc gccacagccg cgaggtctcc agcacgttgg
      181 ccttgtcgac ggaggtgagc ttgccgcgcc gtgcgcgcgc cagggtgcag gcatgccgca
      241 ccacgcgttc gatttcggcc acgctgtagc tgcacaggtc gctggcagcg ccggcatcgc
      301 gggtcttgtc gccgaagtag atgccgccgg tgagttcgcg caccaccacc aggtccaccc
      361 cgttgagcag gtgcggcttg accggcgagg cgcccagcgt ggcccggtgc ggcttcaccg
      421 ggcgcaggtt ggcgtacagg cccagcgcct tgcggatcgc cagcaggccc tgctcggggc
      481 gcaccttcgc cttcgggtcg gaccacctcg gcccgccgac tgcgccgagc agcaccgcgt
      541 ccgccttgcg ggcggcgtcc agggtcgcgg ctggcaacgg ctcgccggtg gcatcgatcg
      601 cggcgccgcc gatcagccgt tcctcgaaat ggaattcatg gccatagcga cgggcgaccg
      661 cccgcaggac cgaaaccgcg gcggcggtga cttcggtgcc gatgccgtca ccgggcagga
      721 cgaggatgtt cgcgtgcatc aggcagctcc cgccgcgtcg cgcgcggctt cgaatcgttg
      781 gatggcaggg tgtcgggtga gcaggtagcc gagctggtcc acgccctcga gcaggcaggc
      841 ctgggcgaac gcgtccagcg ggaatccgaa gcggcggccg tccggcagtg ccagcgtgcg
      901 cgtggcgatg tcgatggcca gttcgatgcc gggttgcgcc agcagttcct cgacgatggc
      961 ctcgtccagc accaccggca gcaggccgtt cttcagcgcg ttgctgcgga agatgtcggc
     1021 gatctggctg ctgatcaccg cgcgcaggcc gaggtcgagc agnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnaccagcgt gcgcgactgc aggtgcctga acggctccat
     1201 cacgccgcct cccggtgcag gaacaaccgc gggtcggtga tctcgccggt caccgcgcat
     1261 accgccgcgg tcagtggcga cgccagcacg gtgcgcgcgc ccttgccctg gcggccttcg
     1321 aagttgcggt tgctggtcga aacggcgagc tgccccggtt gcaccaggtc gccgttcatg
     1381 gcgatgcaca tcgagcagcc gggctcgcgc cactcgccgc cggcggcgcg gacgatggcg
     1441 tcgatgccct ccgcttcagc gtccttcttc acctgctcgg agccgggcac caccagcatg
     1501 cgcacgcgcg gatgcacctt gcgcccgcgc agcacgttcg ccgcggcgcg caggtcgctc
     1561 aggcgcgaat tggtgcagct gccgacgaac accacgtcca ccgcgcgccc ggccaggggc
     1621 gactgcgggt cccagcccat gtagtcgcgg gcttgggcat cgtcgctgtt ctcgtccgcg
     1681 ggaatgcacg cggcgatcgc ggcgacctgg cccgggtgcg tgccccaggt gatggtcggc
     1741 gcgatgtcgg ccgcgtcgac atgcacctcg cggtcgaagc tggcgccgtc atcgctgcgg
     1801 aattcgcgcc agttcgccac ggcgcgctcg aaggcttcgc cccgcggtgc gcgcggggtg
     1861 tggcgcagga actcgaaggt ggtgtggtcc ggcgcgatca gccccgcgcg cgcgccggcc
     1921 tcgatcgaca tgttgcacac ggtcatgcgc tcgtccatcg acattgcgcg gatcgtgctg
     1981 ccgcggtact cgatgacatg gccggtgccg ccgttgaccc cgatcacgcc gatcacgtgc
     2041 aggatcaggt ccttggcgct gacgccgtcg cccagtgcgc cgtcgatggt gatcgccagc
     2101 gtcttcggct tgcgctgcag caggcattgc gtggccagca cgtggccgac ttccgaggtg
     2161 ccgatgccga acgccagcgc gccgaacgcg ccatgcgtcg cggtgtggct gtcgccgcag
     2221 acgatggtca tgcccggctg ggtgatgccg agttccgggc cgatcacgtg cacgatgccg
     2281 cggtgcgcgc tgccgaagtc gaacagttcg acgccgaagg cagccgcgtt ctcgcgcagc
     2341 ttcgccacct gcgcctccgc ttccgggttc gcgtagggca gcttgccggc cgcatccgcg
     2401 ggcagggtgg gcgtcgaatg gtcgagcgtg cccttggtgc gctcgggcct ggccaccggc
     2461 aggccgcgcg tggccagttc ggtgaacgcc tgcggcgagg tcacctcgtg gatcaggtgc
     2521 aggtccacgt acagcaccgc cggcgcggcg tcggtctcgg ggctgaccag gtgcgcgtcc
     2581 cacagcttgt cgaacagggt acggggcggc atcacgcagc ctccacgcgc ggtggcgcca
     2641 ggcgcatgcg cagcacccgg ttggccacgt ccagccaggc gatcgcgctg gcctccagca
     2701 cgtcgcggct ggtgccttgc ccggccatgg cctcgccatc gatgcggccg cccaggttgg
     2761 cctcgccgcg cgcgtcgcgg ccgatgccca tgctgtgcac ctcgtagctt tccagcacga
     2821 agtcgacccc ggtggcctgc gccagcgcgg cgaacagggc ggcaaccggg ccttcgccct
     2881 cggcgctctg catgacgtgg cggccgtcgg gatcggacag gtccacgtgc gcgcgggtgc
     2941 gctggccgta gtcggtcagg ctcaacgcgg agatgcgcca gccggggccg tgctgctcgc
     3001 cctcgaccag ccgctgcagg tcgccgtcgt tgatcacgtg ctgggtctgg gtcagcgcct
     3061 tggcgccggc aatcacctgg tccagctgtt cgtcctccag cacgtagccg agctggcgca
     3121 ggcggtcggc gaccgcggcg cgtccgctgt ggcggcccag caccagttgc gaatccgccc
     3181 agccgacgtc ctgcggacgc atgatttcgt aggtgtcgcg gttcttcagc atgccgtgct
     3241 ggtggatgcc ggattcgtgc gcgaatgcgt tcaacccaac gatcgccttg ttgcgctgga
     3301 cctggatgcc ggtgatccgc gacagcaggc gcgaggtctg cagcagcttg cgggtgtcga
     3361 tgccggtatc cgcgccatac cagccgccac gtaccttcat cgccatcgcc agttcctcca
     3421 gcgcggcatt gcctgcgcgc tcgccgatgc cgttgatggt gcattcgacc tggcgcgcgc
     3481 cgccctcgat cgccgccagc gaattggcca ccgccaggcc caggtcgtcg tggcagtgcg
     3541 cgctgaacac cacgccgtcg gcgcccttga cgttcgcacg caggtattcg aacagcgcgc
     3601 ggatctcggc gggggtggcg tagcccacgg tgtccggcac gttgagcgtg cgcgcaccgg
     3661 cctggatcgc ggcatcgaaa atttcgacca ggaactcgcg ctcggtgcgg aacgcatcct
     3721 cggcggagaa ctcgacatca tcgacgtgcc tgcgcgccag ctcgacgccc atcaccgcat
     3781 gctcgatcac ctgcccgcgg ctcatgttga gcttgtgctg gcgatgcagc gggctggtgg
     3841 agatgaaggc atggatgcgt ggcgcggcgg caccgtccag ggcgcgcgcg caggcttcga
     3901 tgtcggccgg gtggcagcgg gcgagggcgg cgatggaggc gccgcgcacc tcgcgggcga
     3961 tcgcctgcac cgcgccgaag tcagcagggg aactgttggg gaaaccggct tcgatcacgt
     4021 ccacgccgag gtcttccagc gcgtgggcga aggcgatctt ctgcggcgcg ctcatggtga
     4081 agccgggcga ctgttcgccg tcgcgcaggg tggtgtcgaa gatcctgatc cggtgcggct
     4141 gggtgcgatc cgtggtggcg ctgcgattca tgcggggacc atctcgagaa tgtgggtgcg
     4201 tgcttttgtc tgctccctcc cttttgcgaa gcgaagggga gggttgggga ggggtgcttt
     4261 cgccatgcgt gccaggagca ccccctcccg gcctccccct gcttcgcaag gggaggtggc
     4321 aagtgcaggc tcgcgatcgc gctgccgcgg cttgcgcggc gggcgcggcc gcacgtcgga
     4381 cagcaggcgg gccagcacgc cggcatccag gttgccgccg gaaaccaccg cgcacttgcg
     4441 cttgccggct acccgccggc ccgcggccag cgccagcgcg ccggcgccct cggcgatcac
     4501 gtgttcctcc agcgccagcc gcaccagggt ttcgcgcagc tcggcctcgc gcacgatcac
     4561 cacgtcgtcc agcaggtcgt gcaggagtgc gcgggtcagc aggccgggtt ccttcaccct
     4621 cacgccgtcg gccagggtgg cggccggatc gcgcaggctg gtttcgccgc gcagcgcgcg
     4681 tgccatggcg tccacgccct cgacctgggc gccgatgatg cggacgccct gcgacttgag
     4741 cgccagcgcg acgcccgacg ccaggccgcc gccgccgatg ggcaccagca ccacgtcggg
     4801 gctgaacgcc gccgcgatct ccagcccgac cgtgccttgc ccggcgatca cgtccgggtc
     4861 gtcgaaggcc gacaacaggc gatagccgtt gttcgcggcc agttcgccgg cgaacgcctt
     4921 ggcttcgtcg tagctgtcgc cgtgcaggcg cacggtggcg ccccagtgcg ccaccccggc
     4981 gatcttggtc tccggtgcgc agcgcggcat caccgtgatc accggcatgc gcaaccgata
     5041 accggcccag gccaggccct gcgcgtggtt gccggcggag gcggcgatca ccgtccgtgc
     5101 gtcgccgcgt tcgcgcgcgg cgagcagggc gttcaacgcg ccgcgtacct tgtaggagcc
     5161 tgtgcgctgc aggttctcca gcttcagcca gcagccgaag cgttccgcgt agtggaccgg
     5221 ggtggcgtcc aggtagcgcc gcagccgcgc atgcgccgcc agcacgtcgc cggcgccggg
     5281 catgcgtaca gtgctggcta cgtcgctctg catcggctca ggccttggtc agccagcggc
     5341 cggaccagct gctctcgccg cgcacggtgg ccgcgtacac cgcggcgatc tcgcgcgtga
     5401 gcgggctgtt ggcatcgaag cggcggccgt cgatctcggc gatcggcgcg acctcggcgg
     5461 cggtgccgca ggcgaacacc tcgtccgcgt ccagcagttc ctccatgctc accgggcgcg
     5521 agtccgcgcc ggtgatccgg accagggtgt cgcgggtgat gccgggcagt gcgtcgcggt
     5581 gctcgaccgc ggtgatcccg ccatcgcgga ccaggaacag gttggcgccg gtgcattcca
     5641 ccgcgtagcc gtcgtcgtcg acgaacatcg cttcgtcgta gccgcgcagc ctgacttcgc
     5701 gcttggccag gatcgaattg acgtagccgc cgcacagctt cagcggcggc agcgaactgg
     5761 ccgggttgcg ccgccactgc gaaaccgaca cccgcgtgcg gttgccgttg aggtgcaggt
     5821 tggtggccga agtggcgacc atcaggtggt gcgaacggcc ggtgacatcg aggccgaagc
     5881 cgccgtcgcc gtaccaggcc agcgggcgga tgtaggcatc gcccaggcgg ttggcgcgca
     5941 gcgtctgcag caccgctgca gtggcctcgg cgacgtcgaa gtccatgccc agcaactcgc
     6001 atcccttgcg catgcgctcc aggtgctccg gcaggcggaa caccgcggcg ccatcgcggg
     6061 tggcgtaggc gcggatgccc tcgaacacgc cgctgccgta gtgcagcgca tgcgtgccca
     6121 gcgtggcctg cagcttcgtc gcgtcgtgga gttcgccgtc gaaccagagc cgtgactccc
     6181 tcccttgcgc agcagggggt gaagcggcgc gttcgcgaac catcggttcg cgcgccggtg
     6241 aacgcacatc ggcggtgccg gtgggccgtg cgggccgggg aggggtgctc tggtcgctgc
     6301 gcgcgccctc gccacgcgat tccgcggcct gtccgtcgtt cagctgcatg gctgcacctg
     6361 cacgtgcagg cagtcgtaca gcttggccag ttgcgcgtgc agggtctcgg cggggcgttc
     6421 gccttccacc gtcatctgca ggtgccagcg gtcgccatcc gtgagggttt cgccttccac
     6481 cgcgaccggg cggaagccgc ggcgttcggt ggtgccaagc acgcgcgcga gcgcgccttc
     6541 ggcatggcgc agggtgaggt cgagccggta gcgcatgtca ggcctcctgg acggacgtgg
     6601 attcggggag cgggttgctt gcgcggatcg cgggtgcggc ttccgtggcg gcgtcttcca
     6661 gcatttcgct gttgctgcgg ttaggcggca ccagcggcca gacattcgcc gcggtgtcga
     6721 tggcgacgtg cagcagggcc gggccttccg ccgccagcag cgcctgcagc gccgcgtcca
     6781 tgccgtcggc gcgatccagg tgcagggcct ggatgccgaa cgcgcgggcg agggcgacga
     6841 aatccgggtt gtcggacagg tcgacttccg aatagcgctt ttcgaagaac agttcctgcc
     6901 actggcggac catgcccagc gcctggttgt cgagcagcac gatcttcacc ggcagctggt
     6961 agcggcgcag ggtggccagc tcctggatgt tcatctggat cgagccatcg ccgctgacgc
     7021 agaccacgcg cgcagcgggg tcctgcagct gcgcgccgat cgcggcgggc aggccgaagc
     7081 ccatcgcgcc cagcgcgccg gaggtcaggt gcttgcgcgg atggtccagc cgccagtgct
     7141 gggccaccca catctggtgc tggccgacgt cgcacgcgac cactgcatcc ggcgccagtt
     7201 ccgacagccg cttcagcagc gccggcgcga acacgctgtc gccgggggcg tcgtagcgcg
     7261 ccgcgtgttg ttgcgcgcgt tgccggcatt gcgcacgcca ggccttgcgc gcctggccat
     7321 tgcggccatg caggtgggcg gcgcagggca gggtcagccg ggccagggtg gtgcggatgt
     7381 cgccgcgcac gccggcgtcc gcgcagcgca gcttgccgat ctcgcacgcc tccaggtcca
     7441 tgtgcacgac ccgcgcgtgc ggcgcgaatt cggccagctt gccggtggcg cggtcgtcga
     7501 agcgggcgcc gaccaccacc agcaggtcgc tctcctgcac cgccaggttc gccgcgcgcg
     7561 agccgtgcat gcccagcatg cccaggttca acggatgcct ggtcggcaac gcgcccaggc
     7621 ccttcagggt cagcacggtc gggatctggg tggcatccac gaaggtgcgg aagtcctgca
     7681 ccgcatcgcc cagggcgatc ccgccgccgc cgtagagcac cggccgctgc gcctgcgaga
     7741 tcagcgccag cgcctcgtgc agcgatgcgt ccttcggtgc cgcggccgca tccaccggca
     7801 gcggcgcgtg cgccggcagg tgggcggcag cggccatctg cacgtccttc ggcaggtcga
     7861 tcaggaccgg gccgggacgg ccgctgcgcg ccagccggaa cgcctcggcc accatcatcg
     7921 gcaggtcttc aacccgccgg gcgatgaagc tgtgcttgac catcggcagg gtcatcgcga
     7981 acacgtccag ttcctggaac gcgtcggtgc ccatcagggt ggtcggcacc tgcccggtga
     8041 tcaccaccat cggcaccgaa tccagcatcg catccgcaat cccggtgacc aggttggagg
     8101 caccggggcc ggaggtggcc acgcacacgc ccacccgccc gctggcgcgc gcatagccgt
     8161 tggcggcgaa cgccgcgccc tgctcgtggc gcaccagcac gtgcttcagc gccggggtgc
     8221 catgcagggc gtcgtagaac ggcatgatgg tgccgccggg atacccgaac agcgtatcca
     8281 cgccctccgc gaccagtgcc tgcgccagcc agcgggcacc gttcatcagg cggcctcgct
     8341 ctgcggcggt gtttcgctgg cggtggcggg cttcgccgcc gccggctgcg acgacgccag
     8401 ccatttcatg ttggcgcgca gcttcttgcc gatggcctcg accggatgct cgaggtcggc
     8461 ctgcttcatc gccctgtagt tggcgttgcc ggcggcgtat tcggcgatcc actggcgggc
     8521 gaaggtgccg tcctggattt cggtcagcac cttcttcatc tgcgcgcggg tgtgggcgtc
     8581 gacgatgtag tcgccgcggg tcagcgcgcc gtactgcgcg gtctcgctga tgaactcgtg
     8641 catgcgggtg atgccgcctt cgtagaacag gtcgacgatc agcttcagtt cgtgcaggca
     8701 ctcgtagtac gcgacttccg gctggtaacc ggcttccacc agggtttccc agccggcctg
     8761 caccagcttg gtggcaccgc cgcacagcac cgcctgttcg ccgaacaggt cggtctcggt
     8821 ttcttccttg aaggtggtcc ggatcgcgtt ctggcgggcg ccgccgatcc cggcgcaata
     8881 ggccagcgcc agctgttcgg cgttgccgct gcggtcctgg tggactgcgt acaccgccgg
     8941 cacgccacgg ccgatctcgt attcgcggcg gaccagcgcg cccgggccct tcggcgccac
     9001 cagcaccacg tcgaggtcat cgcgcggggc gatctgctgg tagtgcacgt tgaagccgtg
     9061 cgcgaacagc aggcaggcgc ccgccgcgat gttcggctcg atcacctcgc cgtacagctg
     9121 cggctggacc atgtccgggg tgaggatggc gacgatcccg gcgtccttca ccgcctcggc
     9181 gggcgccttg acggtgaagc cgtcggccct ggccttgatc tcggtcgggc cgccggggcg
     9241 caggccgatg gtgacgtcga agccggagtc gcgcaggttc agcgcatgcg cgcggccctg
     9301 gctgccgtag ccgacgatgg tgactttggg tttggcggtg gtgctggtca tggcggttcc
     9361 ttgggcggaa gtggacgatg gctggaggag gggtcaggcg gggatcgctg cgtcggtggc
     9421 cgcatcgagc aagctgctgg cgatgcgtcg ggcgtcgtcg tcgtgggcat cgagcgggcc
     9481 gggcgaggtg accgcgccgc gcgaggcgga gccgacatca cgcgcgaact tggccagcgc
     9541 gccgcagcgc acgcgcggcg ccatccgcgc aggcgtgcgt tgcgacaggt cggcagccac
     9601 gtcgatccgg cgcgatgcca cgtcgatgcg cacgcggtcg ccatcgcgca gcttcgcgat
     9661 cggcccgccg cgcgcggctt ccggcgccac gtggccgacc atgaagccgt gggtggcgcc
     9721 gctgaagcgg ccatcggtga ccagcgcgac atcgttggac aggccctggc cgaccaatgc
     9781 cgcggtcacc gccagcatct cgcgcatgcc gggtccgccg gccgggccct cgaagcggat
     9841 caccaccacg tcgccggcga cgatggcgcg cgcctgcacc gcggcgaacg cggcctcctc
     9901 ggaatcgaat acccgcgccc ggccctcgaa gcgctcgatg ccgtggccgg ccagcttgac
     9961 gatgcagccc tccggcgcga ggtcgccgta caggatcgaa tagccgccgc gcggcttgat
    10021 cggatcggac accgggcgca ccacgtcctg cgccggcggg gtttgcgcgg catcggcctg
    10081 cgcgaaaaag ctgcggccgg cgaccgtggg gatgtcctcg atcaggccgg ccgcctgcag
    10141 ttcccgcgcc accagcgcgg ttccgccttg cgcgtgcatt tccgccgcgg tgtagcggcc
    10201 gcccggcttc aggtcggcga tcaccggcgt gctggcgctg gcggcctcga attcctccag
    10261 gtcgaaggcg acgccggctt cgtgcgcgat cgccagcagg tgcagcaccg cgttggtcga
    10321 gccggcggtg gccgagaccg cgcgcgccgc gttgcgcagc gcacgggtgg tcagcagggt
    10381 gcgcggggtc ggcccgccgt cgcgcagcag cgccatcgcc agctcgccgc aggcgtagcc
    10441 ggcatcggcc ttcgccgggt cggtggcggg gatgtcgttg gcgcccagcg gcgacagccc
    10501 gaggaaggtc agcaccatcg ccatcgtgtt ggcggtgaac tggccgccgc aggcaccggc
    10561 gccggggcag gcatgggatt cgatcgcgtg cagcccggca tcgtcgatgc gcccggccgc
    10621 gtgcgcgccg accgcctcga acacgtcctg cacggtcagc ggcttcggcg cctcgccatc
    10681 cgtcgtcggg cagtgcccgg gcaggatggt gccgccgtag aggatcacgg tggggatgtc
    10741 gagccgggcc gccgccatcg ccgccgccgg gatggtcttg tcgcagccga ccagcagcac
    10801 catcgcgtcc aggcagtggc cgccgaccgc cagctcgatc gaatcggcga tggtctcgcg
    10861 cgagggcagc gaggcgcgca tgccgtcgct gcccatcgcg atgccgtcgg tgaccgcgat
    10921 ggtgttgaac tcgatcgggg tgccgccggc ggcgtcgatg ccacggcgca cgtgctgcgc
    10981 caggtcgcgc aggttgatgt tgcaggggga gacgtcggac caggtgtgca ccaccgccac
    11041 gaacggcctg gcgatggcgg cgtcgtccag cccggtggcg cgcagcatgg cgcgcgccgg
    11101 cgagcgggtg ggaccttgct tgatggcatt gcttctcacg gatggcattc cttggtgttg
    11161 ccgcggaaac ccgtgccgca aaaacgaaaa ccccgcgcct tttcgggcgc ggggtcttgg
    11221 ttctttcgac ctgggtgcgt gggtgttgcc tacacgaacc cgactcctgc gcctgattgg
    11281 ccggaaataa tgagtacgag tacaagcacg agcgtcgcgg caagcgcgac gtgggcgaac
    11341 gacagcggca gcgcgcgcgg gcccgcggcg accgcgtcct gcgacggggt gcgtgtggcc
    11401 tgcggatgga tgttgcgctg cgtcatgact gcagggaaac acaggtccgc gatcgctgtc
    11461 aagcccctgc gcgtggccag ccgcaagcac tgcgcgggcc ggattcccgc ggacccggta
    11521 ccggatttcg tgggcgctgc ggcatgacgc cggcgcaggt tccggtctag actcgggccg
    11581 caagcgatgg ccggcgcatc cggctttccg acaggccctg aaccggcttg cctgcagcga
    11641 gggtcgcggt gaagccgacc gatattcgca atcccgacgt cttccacaag gtcgtcgact
    11701 gccagtgggc ctgtcccgcg cacaccccgg ttcccgaata catccgcctg atcgccgccg
    11761 gccgccacgc cgacgcgtac atggtcaact ggcggtcgaa cgtgttcccc gggatcctcg
    11821 gccgcacctg cgaccgcccg tgcgaaccgg cgtgccggcg cggacgggtc gagcacaaca
    11881 acggccccgg cgcgatgcgc gacaaccccg agccggtggc gatctgccgg ctcaagcggg
    11941 tcgccgccga ccacaagggc gacgtgcgcg cgcgcatgcc gaagccggcc agccagcgca
    12001 acggcaagcg ggtggcgtgt gtcggcgccg ggccgtcgtc gttgaccgtc gcccgcgacc
    12061 tggccccgct gggctaccag gtcaccctgt tcgaggccga tcccaaggcc ggcggcttca
    12121 tgcgcaccca ggtgccgcgt ttccgcctgc cggagcaggt gatcgacgag gaatgcggct
    12181 acatcctcga cctcggcgtg gagttggttg ccggtcgccg gatcgaatcg atgcgcgcgc
    12241 tgctggccga agggtacgac gcggtgttcg tcggcagcgg cgcgccgcgc gggcgcgacc
    12301 tgcgcatccc cggtcgcgac gaggccgcgg cgaacatcca ccttggcctg gactggctgg
    12361 cgaacgtgta tttcgggcac gtcgccgcgg tcggcaagcg cgtgctggtg ctgggcggtg
    12421 gcaacaccgc gatggactgc tgccgcagcg cgcgccggct gggtggcgag gacgtcaagg
    12481 tgctggtgcg ttccggcttc gacgagatga aggcgagtcc gtgggagaag gaggatgcga
    12541 tccacgaagg catcccgatc ctcgactgcc gggtgccggg tgccttcgtc cacgaaggcg
    12601 gcaagcttac cggcatgacc ttccagcggg tgcgccccga attcgacggc aacggccgcc
    12661 gcatgctggt gccgaccggc gagccggacg aattccatgc ctgcgacgag gtgctgatcg
    12721 cgatcgggca ggagaacgcg ttcccgtgga tcgagcgcga ttgcgggatc gcattcggcg
    12781 aatgggacat gccggtggtc gatccgctga cccaccagtc gtcgctgccg cacgtgctgt
    12841 tcggcggcga tgcggcgttc ggtccgaaga acatcatctg ggcggttgcg cacggacacg
    12901 ccgcggcgac cagcatcgac cgcctgctgc atggcgagga cccgcgcgaa cgcccggatc
    12961 cgctggtgcg gctgacctcg cagaagatgg gcatccacga gtggagctac gacaacgggg
    13021 tgtcggcgga ccagcgtttc gcggtgccgt ggtcggagga agccaggaaa ctggacagca
    13081 tcaaggtcga ggtggagctg ggcttcgatg ccgccaccgc gctcaaggag acccagcgct
    13141 gcctcaactg cgacgtgcag acggtgttca cccgcgacaa gtgcatcgag tgcgacgcct
    13201 gcgtcgacat ctgcccgacc gactgcatca ccttcaccgg caacggcgag gaggccgcgc
    13261 tgcgcacccg gctgaccgcg ccggcgctca acgcggcaca ggacctgtac gtgtccgagc
    13321 cgctgccgac gatgcgggtg atggtcaagg acgaggacgt ctgcctgcac tgcggcctgt
    13381 gcgcggagcg ctgcccgacc ggcgcctggg acatgcagaa attcctgttg ctggaaaccc
    13441 atgcgggcac gcgctgcggc ggttcgccgg cacgggaggc cgcatgagca tggcgatcga
    13501 tccacccgcg gtggcggcgt tgcaggcggt caacgatttc gtggtcaagt tcgccaacgt
    13561 caacggctcc ggctcggcct ccgccaacga gctgttcgcg aagtcgatcc tgcgcatggg
    13621 ggtgccggtc agcccgcgca acatcttccc ctccaacatc cagggcctgc cgacctggta
    13681 cgaggtacgc gtcagcgagg ccggctggct gggccgccgt ggtggcgtcg acatgctggt
    13741 ggcgatgaac ccgcagacct gggacgacga cgtcagggaa ctcgcgccgg gcggttatct
    13801 tttttacgac agcagcaagc cgctgccgaa gtcgaagttc cgcgacgaca tcaccgtcat
    13861 cggcgtcccc ctgaccgaac tgtgcaacgc cgcctaccag gacccgcgcc agcgccagct
    13921 gttcaagaac atcatgtacg tcggtgccct gggcgcgttg ctggacatcg acatggcggc
    13981 gatcgaagcg ctggtcggcg aacagtacag gaccaagccg aagctgctgg cgcccaacat
    14041 cgacgccctg cacatgggcc gcgggtgggt gcacgagcac ctgccgcacc cgatcggcct
    14101 gcgcgtgcgc aaggccgatg cggtcggcga ccgcatcttc gtcgagggca atgccgcggc
    14161 cgcgctcggc tgcgtctacg gcggggccac cgtgtgcgcg tggtacccga tcaccccgtc
    14221 gtcttcggtg gccgaggcct tccagaggaa ctgccagaaa ttccgcatcg acgacgcggg
    14281 ccgcgcgcgc ttcgcggtga tccaggccga ggacgagatc gcctcgatcg gcatggtggt
    14341 cggcgccggc tggaacggcg cgcgcgcgtt cacctcgacc tccggccccg gcatctcgct
    14401 gatgaacgaa ttcatcgggc tggcgtactt cgccgagatc ccggccacca tcatcaacgt
    14461 ccagcgcggc gggccctcga ccgggatgcc gacgcgcacc cagcagtcgg acgtgctggc
    14521 ctgcgcgcat gcatcgcatg gcgataccaa gcatgtgctg ctgttcccgg aggacccggc
    14581 cgagtgcttc gggttcgccg ccgaagcgct ggacctggcc gatcgcctgc agaccccggt
    14641 gttcgtgatg agcgacctcg acatcggcat gaaccagcgc ctgtgcgcgc cgttccgctg
    14701 ggacgatgcg cgccgctacg accgcggcaa ggtgatgacc gccgaggagc tggaggcggg
    14761 gcgcgaattc gcgcgctaca aggacgtcga tggcgacggc atcccgtacc gcaccctgcc
    14821 gggcacgcac ccggacaagg gcgcttactt cacccgcggc acctcgcgcg atccgcaggc
    14881 gaagtactcc gagcgcggcg ccgactacct ctacaacatg cagcggttgc tgcacaaatt
    14941 cgagaccgcc aaggcgctgg tgccgcagcc ggtggtggac cagtcggtgc caccggcgcc
    15001 gcaggccgcg atcttctacg gttcgaccag cccggcgatg gcggaggcgg tcgagttgtt
    15061 gcgggacgcc ggcatcgcgc tggacagcgt gcgcatccgc gcgttcccgt tcgcggattc
    15121 ggtgcgggac ttcatcgccg cgcacgaatc ggtgttcgtg gtcgagcaga accgcgacgg
    15181 ccagttgcgc agcctgctgg tggacgaatt cgggatcgac ccggcgcgcc tggtcgcggt
    15241 gctgcattac gacggcaccc cgatcaccgc gcgcttcatc gcaggtgcga tcgaggcgcg
    15301 gttgcgcccg gcccgcacgg aggtggcctg agccatgacc tatctcgcca agcccaggct
    15361 ccaccacccg agcctgcgtt gcaacgcgct cgggttcacc cgccgcgact acgagggcac
    15421 cgtctcgacc ctgtgcgcgg gttgcggcca tgactcgatc tcggcggcga tcatccaggc
    15481 ctgctgggag atggacatcg ccccgcaccg ggtggccaag ctgtcgggga tcggctgcag
    15541 ctcgaagacg ccgacctatt tcctcggcca gtcgcacggc ttcaacaccg tgcacgggcg
    15601 catgccgtcg gtgctgaccg gcgccaacct ggccaaccgc gggctgatct acctgggcgt
    15661 gtccggcgat ggcgactccg cctcgatcgg gatcggccag ttcgtgcatt cgatccggcg
    15721 cgcggtggac atgacctaca tcgtcgagaa caacggcgtg tacgggctga ccaagggcca
    15781 gttctcggca accgcggacc agggctcggt gtcgaagaag ggggtggcca acaacgacag
    15841 cccgctcgac ctcgtcacca tggccctgca gctgggcgcc acctatgtcg cgcgcagctt
    15901 ttccggcgac aagcagcaac tggtgccgct gatcaagggc gcgctggcgc atcgcggcgc
    15961 cgcgttcatc gacgtggtca gtccctgcat cgccttcaac aaccatccgg gcagcaccaa
    16021 gagctacgaa tacgtgcgcg agcacaacga ggcggtgaac cggctggacg tggtgctgcc
    16081 gcgcgcggag atctccgccg aatacgcgga aggcgaactg ctggaaatcg accagcacga
    16141 cggctccgtg ctgcgcctgc gcaagctcga cgcggactac gatccgcgcg accgcatccg
    16201 tgccatgaac ctgctgcagc agcggcaggc gcagggcgaa gtggtgaccg ggctgctgta
    16261 tgtcgccgcg gacgcggagg acctgcaccg ccacctcaat accatcgaga cgccgttgaa
    16321 cgcacttggc gcggaggaac tgaacccggg cgcggaggcg ctggagcgga tcaacgcctc
    16381 gctgcgctga gcggatgccc gccgcgggcg gttggcgcgc gcggatgccg cgtgttccaa
    16441 tagcacccgg agcgggggta ccgccggcat cccgtgccgc ggttgagaca gacccttcga
    16501 acctgatccg gttgagaccg gcgtagggaa gcttcgcgac ggcagcgccc ccggtggccg
    16561 ctgcacgcgc gccgccgctt cgtccccgct ccgcgagcga accgcacagg acgatgtcga
    16621 tgaacgcagt gccctccgaa ctcatccgcc aggccgaaca gctctcggaa gccgtcatcc
    16681 ggccgatccc gggctcgcgc aaggtccacg tcgccggatc gcgcgccgac ctgcgggtcc
    16741 cgatgcgcga gatcgagctg gcgcgcacgc cgaccctgtt cggcggcgag gacaacccgc
    16801 cgctcgcggt gtacgactgc tccgggccct acaccgatcc ggcggtgcgc atcgaccttg
    16861 ccgccggcct gccggcgctg cgcgcgcagt ggatcgccga gcgcggcgat accgacgtgc
    16921 tggagcggat cagctccgaa ttcgggcgcg cgcgcgaggt ggatccgaag ctggccgcgg
    16981 tgcgcttccc cggccgcagc ctgccgcgcc gcgccagggc cggcgccaac gtctcgcaga
    17041 tgcactacgc ccggcgcggc atcgtcaccc cggagatgga gttcgtggcg atccgcgaga
    17101 accagcggat cgaggcggtc cgcgacgcca tcctgctccg gcaacacccg ggggagggct
    17161 tcggcgccag catccagaaa ttcatcaccc cggaattcgt ccgtgacgag atcgcccgcg
    17221 gccgcgcgat cctgccgtgc aacatcaacc acccggaaag cgagccgatg atcatcggcc
    17281 gcaacttcct gaccaagatc aacgccaaca tcggcaactc ggcggtgagt tccgggatcg
    17341 ccgaggaggt ggagaagctg gtgtgggcga tccgctgggg cgcggacaac gtgatggacc
    17401 tgtccaccgg caagcacatc cacgagaccc gcgagtggat cgtgcgcaac tcgccggtgc
    17461 cgatcggcac ggtgccgatc taccaggcgc tggaaaaggt cgatggccgc gccgaggaac
    17521 tgacctggga gatcttccgc gacaccctgg tcgagcaggc cgagcagggc gtggactact
    17581 tcaccatcca cgccggggtg ctgctgcgct acgtgccgct gaccgcgaag cgcgtgaccg
    17641 gcatcgtctc gcgcggcggt tcgatcctgg ccaagtggtg cctggcgcac cacagggaga
    17701 acttcctcta cacgcatttc gaggacatct gcgaaatcat gaaggcctac gacgtggcct
    17761 tctcgctggg cgatggcctg cgtcccggct gcatcgcgga tgccaacgac gccgcccagt
    17821 tcggcgagct ggaaaccctc ggcgagctga ccaaggtcgc gtggaagcac gacgtccaga
    17881 ccatgatcga aggccccggc cacgtgccga tgcagctgat caaggagaac atggacaagc
    17941 agctggccga atgcggcgag gcgccgttct acaccctggg gccgctgacc accgacatcg
    18001 cacccggcta cgaccacatc accagcgcga tcggcgcggc gatgatcggc tggtacggca
    18061 ccgcgatgct ctgctacgtg acgccgaagg agcacctcgg cctgcccaac cggcaggacg
    18121 tgcgcgacgg gatcatggcc tacaagatcg cggcgcacgc cgccgacctg gccaagggcc
    18181 atccgggcgc gcaggtgcgc gacaacgcac tgtccaaggc gcgcttcgaa ttccgctggc
    18241 aggaccagtt caacctcggc ctggatccgg agaaggccag ggaattccac gacgagacgt
    18301 tgccgaagga cgcgcacaag gtcgcccact tctgctcgat gtgcggcccg catttctgct
    18361 cgatgaagat c
//
LOCUS       DAORUK010000024        17886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_2794_length_17886_cov_19.268297, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000024 DAORUK010000000
VERSION     DAORUK010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 17886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17886
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2794_length_17886_cov_19.268297"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PK818_02895"
     CDS             <1..1235
                     /locus_tag="PK818_02895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY81714.1"
                     /translation="VNLTLAFAAWFMVSALVVRLPQVGYTFSPSQLFWLTAMPGLAGG
                     TLRLVHMFLTPMVGTRHVVSLSTLSLLVPLVGWFFAVQDPNVPYWVLLLLSFFAGLGG
                     GNFSSFMPSTSLFFPKRLLGTALAIQAGIGNLGVSIVQFVTPWIIGVALLGGAAFMGD
                     SQTLVKNGVESQVHLQNAAAVFIPFVVVFGITAWLLLKSVPVKANFGEQFDIFKSRHT
                     WSMTSLYMMTFGGFSGLAATFPLLIKQGFGGFEGAPDPLKWAFWGPLVGSIARVIAGP
                     LSDKLGGARVTQWAGIGMVACTVMVAMNVSPDSLDEFPTFVGWMLALFFFAGIGNAST
                     FKQMPMLFAPRQAAGVIGFTAAIAAYGPFLFGMLFAWAFGAFKAATPVFYGLAVFFAF
                     NVVLNWWMYARRGAANPC"
     gene            1255..2568
                     /locus_tag="PK818_02900"
     CDS             1255..2568
                     /locus_tag="PK818_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HQY81715.1"
                     /translation="MSAVPALPSRGQQNLALWGSTFAFTVCFAVWTIFSIIGIQIKKD
                     LGLNDTQFGLLVATPILTGSLIRLFLGVWAEQYGGRIVFSLVMLAGASATWMLTYAQT
                     YPQFLLAALAVGIAGGSFAVGVAYVSKWFPQEKQGTALGIFGAGNIGSAVTKLLAPMV
                     MVAYGWHGVARFWAIGLAITAVLFFLFTKDDPGLARRKAEGIKPVPFAEQMKPLRNLQ
                     VWRFALYYFFVFGAFVALALWLPRYLTGAYGLDVRTAGLIAACYSIPASLFRIVGGWL
                     SDRIGARRVMYWTFGVSALCTFLLSYPSTTYVVDGINGPVEFRVAMGLLPFVVLVFVL
                     GFFMSLGKAAVYKHIPVYYPNNVGAVGGVVGMIGGLGGFILPITFGAMNDALGIWTSC
                     FMLLFVLVSTALVWMHFAIRRMDVSRHPQITSDTDLPEIMPQAGR"
     gene            2576..3223
                     /locus_tag="PK818_02905"
     CDS             2576..3223
                     /locus_tag="PK818_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004903844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HQY81716.1"
                     /translation="MQGPQLDAEQALARLREGNRRFVDHVISLEALLSHARREDHARA
                     QRPFAVVLGCSDSRAPAEFVFDQGLGDLFVIRVAGNIAAPSQVGSIEFAVEQFQVRLV
                     VVLGHSNCGAVRATLAHLQAPGDTSPALRAIVDRIAPGIQPVLSGCGGESDACMPQAI
                     RANVEATIRNLRAQSDYLRYAELQGGLRIVGADYDLSSGAVDFFASQAPAWTNDA"
     gene            complement(3245..3649)
                     /locus_tag="PK818_02910"
     CDS             complement(3245..3649)
                     /locus_tag="PK818_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase subunit alpha"
                     /protein_id="HQY81717.1"
                     /translation="MQHFDDFLAAAGGQPEPQRLLLVFARAECPPDATAAERAAFERG
                     EGGALAPAVCVDKLPEEIASFAALLEESKAAIPDWRILFVAAMDGRGGHPPNSDEAVQ
                     PLQMMVEQVKGGRIARFLAVDRDGRLVELARG"
     gene            3782..4027
                     /locus_tag="PK818_02915"
     CDS             3782..4027
                     /locus_tag="PK818_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81718.1"
                     /translation="MTMPATHSHAPDPIQALRELIAGLDPECEVLVDEISGDVLVRGS
                     IDPAQLDAAIKASGLGMRIADSAGESGGGCCGGCGCA"
     gene            4079..5431
                     /locus_tag="PK818_02920"
     CDS             4079..5431
                     /locus_tag="PK818_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81719.1"
                     /translation="MTTAASDGSLARLRRNLRLGGGWYALYPPPRAFQAFGDDRFIAA
                     IRESNGDPIPPQLALHFDIPAAYRSSFCHPDSRPRGADGSRAEAYRDRLVREVGLVGS
                     LFDRDRDVVQVSLAPGMTRWMSVAQVAELLASLRRHFHADRAADIDLAMTLDPDDLAS
                     APLRDWAALGFNRVGINIAALAGAGAAPSRQREDVARLVAQAREAGFANLRVEVPYGL
                     AGQTDAAFDALVAAVAAARPERVGLRHCPSQDAPDAGATPAGHDAHARMLLAGADALE
                     HAGYLHVGVDLFAQADDPLVRAQRQGRIHRDALGFGAHAATHLVGFGVGAISQLGGCH
                     AQNPLDQPSWEARIDRGHRACNRGVELSEDDHLRAAVLQDILCYGRIGVAQLEAHHRI
                     PFRAYFGDALARLQPLFEDGSLCWDGDAILLDRIARLAAQAIASQFDPLTGATAGGTA
                     "
     gene            5428..6900
                     /locus_tag="PK818_02925"
     CDS             5428..6900
                     /locus_tag="PK818_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HQY81720.1"
                     /translation="MSFHDLRGFLAALERNGRLRRVREPVDPELETTSLSLRALKAGG
                     PALLMEQPLGARHPYLGNLFGHRDRIEAALAGRPLASLRELGELLASIKEPKWPSSLR
                     EALGTWPELAQLAHVAPQRVREAAFQHEVLEGDEVDLSRLPIQRCWPEDAAKLITLGL
                     VVTRGTRQKRQNVAIYRQQVIARNRIILRWLPHRGGALDYASWQHSHPDQPFPLLVAI
                     GADPATLLAAVAPVPDTLSEYEFAGLLRGERTRVWHSAATGLDAPAGCEILLEGHIAP
                     GDTALEGPFGDHTGYYNAQDHFPVMTITRMHLRRDAIYQGSYMGRAPHDEPSVMAMAL
                     NDVFVPILRKVFPEIVDFYLPPEACSYRIAVVSIRKQYPGHARRIMMGIWSYLRQFTY
                     TKFVIVTDDDIDVRDWSQVIWALSTRVDPARDSVIIDKTPIDYLDFSSPSPNLGSKLG
                     LDATNKWPAETTRTWSKPIALDPAVEKRVDALWSQLLQAP"
     gene            complement(6916..7491)
                     /locus_tag="PK818_02930"
     CDS             complement(6916..7491)
                     /locus_tag="PK818_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnrO protein"
                     /protein_id="HQY81721.1"
                     /translation="MLQVPTIPPRARWRPAMAALVLLAVATGCSPRPGAEPAAAAPAA
                     QAPPAAADAGHDAHAAHAAHADAPVDAAPLPPAGQRWATDAPLRAGMARIRSAVEALQ
                     HGLMGHLTEAQQKDAAAQVDAAVADMIANCKLEPEADASLHGLLAPLVAGAGAVREGR
                     FGQPELAAMQDALAQYPQRFDDPGWDQPAQE"
     gene            7636..8634
                     /locus_tag="PK818_02935"
     CDS             7636..8634
                     /locus_tag="PK818_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HQY81722.1"
                     /translation="MQLVCPAGNLPALQAAVDAGANAVYAGFRDQTNARAFPGLNFSD
                     DELRAGVRYAHAKGAKVYLALNTYPDSARLKDWHAAVDKAAAIGCDAIIAAEMAVLDY
                     AARTHPGLPRHLSVQGSATTAPALKYAYERFGISRAVLPRVLSLQQVERLCERSPVAL
                     EVFAFGSLCIMVEGRCQLSSYVTGASPNRHGVCSPAKFVRWDEHTDGSRSVRLNDVLI
                     DQFQPQEPAGYPTVCKGRFEVGNEIFHALEEPTSLNTLELLPRLQQAGVVALKIEGRQ
                     RGVAYVSSVTRTWRKALDRLAADPAHWQLQPDWQAELSRHAEGHQTTLGPYHRAWH"
     gene            8645..9523
                     /locus_tag="PK818_02940"
     CDS             8645..9523
                     /locus_tag="PK818_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008217287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HQY81723.1"
                     /translation="MKLSLGPLQYFWPRERTLAFYREAAAWPLDIVYLGETVCSKRRE
                     LRTAEWIELAQELAAAGKEVVLSSLSLIEAESELGVVDRLAAHGGFKVEANDLSAVQV
                     CRERGTPFVAGPSLNLYNHRALALLVEDGLVRWVPGVEQGETLVRELKQAFEADGGAM
                     PELEVIGWGRLPLAYSARCFTARALDVAKDQCGFRCIDYPDALPLATREGRPFLRING
                     IQVLGEELTDLGPELPALRALGVDILRLYPQAEGMADLVRHFDQARAGDAAAAPLGQR
                     NGYWHGEPGMQVAAAG"
     gene            complement(9530..10993)
                     /locus_tag="PK818_02945"
     CDS             complement(9530..10993)
                     /locus_tag="PK818_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY81724.1"
                     /translation="MSGDAPIIVVGGGIAGLSAALAAAPAPVLLLTRRPGAAGAASTL
                     AQGGIAAALGAGDTPAAHARDTCVAGSHHNDVAMVELLTASAPAAIAWLQAIGVDFDR
                     DADGRLQLGREGGHDRARIAHAGGDGTGAAVMRALVAAARAQPRIRRIAGCDVDALAV
                     RDGQVVGVSATLDDGSRQRFDGRAVVLATGGIGGLFAATSNPADADGGGLALALAAGA
                     AARDLEFVQFHPTALAVPGLHSLPLVTEALRGAGARLLDRDGQPLMAGVHPLGDLAPR
                     DIVARRVAAAVHAGGAWLDARHIGVAWPTAFPTVLAACLAHAIDPRLQPIPVTPAAHF
                     HMGGLATDADGRTSLPGLFAAGEVACNGVHGANRLASNSLLEGVVFGRRLGQWLAAVS
                     GAENRGGEAAWVGLGPALPPPALQRLRALMGAAMGPVRNHAGLQAALDECEGLAAEGW
                     QAGLARALLAAALQRRRSLGAHYRDDDASMAGPVAAA"
     gene            complement(10990..12024)
                     /gene="nadA"
                     /locus_tag="PK818_02950"
     CDS             complement(10990..12024)
                     /gene="nadA"
                     /locus_tag="PK818_02950"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HQY81725.1"
                     /translation="MTRQDAFRPRWTPEIAERHAALGARLEQLVPCLEVPLHLAWIDA
                     IQQLKRERGAVIMAHSYQSPEIFHGVADITGDSLALAQAAATCEADTIVLCGVHFMAE
                     TAKILAPDRTVLIPDLEAGCSLAASITADDVRALRARHPGLPVVSYVNTTAAVKAESD
                     ACCTSANASEVVEAVAAEFGVDTVIFLPDRFLGAHVASQTKIRLVLWDGSCEVHETFT
                     AQQVRHTREQFHARVVAHPECPPEVLAEADFVGSTTAMGSWLEREKPERVALITECSM
                     ADNLKSRFPGIEFVKPCNLCPYMQRITLPNIHASLLERKYVVEVPAGIAAGARRALER
                     MLAVGRRDRV"
     gene            complement(12083..12934)
                     /locus_tag="PK818_02955"
     CDS             complement(12083..12934)
                     /locus_tag="PK818_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HQY81726.1"
                     /translation="MTRPVPGLTPVRTAIDGIDDGLVLLLAARARLAQLAGRLKLHAG
                     ARGRDPGRERQVRERAAALAVRVGVAPEVASGVVGLAIGEACRAQALASDLDQGPPAA
                     AGRMMAATMDAQPESPLPAANPLLRLIPPPRRLAPLLRAVPPSLRRRVIERAMAQALA
                     QPLAEGAIEFMDGRRLGIEVSDLGIGWVVELQGNRLVVVDAPAEATVRGTATDLLLLA
                     GRREDADTLFFQRRLVLTGDTELGLTARNVLDRLPWEQVPLGLRIALNRGARLADAAR
                     AAYHARA"
     gene            complement(13068..14936)
                     /gene="cysN"
                     /locus_tag="PK818_02960"
     CDS             complement(13068..14936)
                     /gene="cysN"
                     /locus_tag="PK818_02960"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HQY81727.1"
                     /translation="MAPDIAAYLQQHESKGLLRFITCGSVDDGKSTLIGRLLHDTKLL
                     LDDQVAALEADSRRHGTQGGELDFALLVDGLAAEREQGITIDVAYRFFGTERRKFIVA
                     DCPGHEQYTRNMATGASTADLAVVLVDARKGLLTQTRRHSYIASLLGIRHVLLAVNKM
                     DLVDYRQDVFDSIVAGYRDLATQLGIANVQAIPLSALRGDNMVDGSAAMPWYRGPSLL
                     QHLETVQVEPATQATGFRLPVQWVNRPNQDFRGFAGTVAGGRVAVGDEVVVLPSARRS
                     TVARIVTAGGDLAHAGDGQAVTLTLADEVDASRGDVIAAAIDPPEVADQFAAHLLWMG
                     DERLLPGRPYLLKLGTRTVGASVTEIKHKVDVNTQEHLAAKHLELNEVACCNLHLDQP
                     LAFEPYARNRALGAFILIDRQSNATVAAGTLDFALRRAGNIHWQHLDVDKAARARIKH
                     QSPRCLWFTGLSGAGKSTIANLVEKRLLAMGQHTYLLDGDNVRHGLNKDLGFTDEDRV
                     ENIRRVAEVAKLMTDAGLIVLVSFISPFRAEREMARALFEPGEFLEVFVDTPLAVAEQ
                     RDVKGLYAKARAGQLRNFTGIDSPYEAPEHPELHLHADRASPVELAGEVIRALLPD"
     gene            complement(14936..15841)
                     /gene="cysD"
                     /locus_tag="PK818_02965"
     CDS             complement(14936..15841)
                     /gene="cysD"
                     /locus_tag="PK818_02965"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HQY81728.1"
                     /translation="MDAAPSHLDRLEAESIHILREVAAGFRNPVMLYSVGKDSSVLLH
                     LLAKAFHPARPPIPLLHVDTGWKFREMIEFRDRRAAETGIELRVHTNPDGVARGIGPI
                     SHGATVHTDVMKTQGLKQALDKWGFDAAIGGARRDEEKSRAKERIFSFRNDQHRWDPK
                     NQRPELWNLYNTRIHAGESVRVFPISNWTELDVWLYIYRENIPVPSLYLARERPVVER
                     DGALILVDDERLPLRADEVPALKRVRFRTLGCYPLTGAIESGADTLEKVIAEMLVATS
                     SERQGRVIDHDPAASMEKKKQEGYF"
     gene            complement(15878..17503)
                     /locus_tag="PK818_02970"
     CDS             complement(15878..17503)
                     /locus_tag="PK818_02970"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HQY81729.1"
                     /translation="MSRYAVYAASLLLTLASLLLALRMPGWWAGVVLFGGLAALGTID
                     LLQRKSTLRRLYPLLAHFRYGLESIGPEMRQYFIESDLAEVPFSRQQRALVYQRAKRL
                     NDVRPFGTQQDVYGIDYEWINHSLAPAAIASPDFRIVIGAECAQPYSASVFNISAMSF
                     GSLSAAAIRALNAGAKRGGFYHDTGEGSISPYHREAGGDLVWEIGSGYFGCRNEDSSF
                     SEERFTANARDAQVKMIEVKLSQGAKPGHGGVLPAAKVSAEISVTRGVPMGRDCVSPA
                     RHSAFSTPLELLHFIARLRQLSGGKPVGFKLAIGHPWEWFGIAKAMHESGILPDFIVV
                     DGAEGGTGAAPAEFIDHVGVPMHEALMLVHNTLVGLELRDRIRIGAAGKIVGAFDIAR
                     TLAMGADWCNAARGYMFALGCIQSMACHTDRCPTGIATQDPGRWKRLDVPDKASRVAE
                     FHANTLHALRDLLCAAGLEHPDQLGPEHILRRVSPTHVRSLGALYRFLEPGDLLGGRI
                     PGHAVFQDFWQAARSDAFMPPENVLRLRSGKAY"
     gene            complement(17523..17609)
                     /locus_tag="PK818_02975"
     tRNA            complement(17523..17609)
                     /locus_tag="PK818_02975"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17573..17575),aa:Leu,seq:taa)
BASE COUNT         2517 a         6392 c         6297 g         2680 t
ORIGIN      
        1 cggtcaacct gaccctcgcc ttcgccgcct ggttcatggt ctcggcgctg gtggtacggc
       61 tgccgcaggt cggctacacc ttcagcccca gccagctgtt ctggctgacc gcgatgcccg
      121 gcctggccgg cggcaccctg cgcctggtgc acatgttcct gaccccgatg gtcggcaccc
      181 gccacgtggt gagcctgtcg acgctgtcgc tgctggtgcc gctggtcggc tggttcttcg
      241 cggtgcagga cccgaacgtg ccgtactggg tgctgctgct gctgtcgttc ttcgccggcc
      301 tcggcggcgg caacttctcc tcgttcatgc cgtccaccag cctgttcttc ccgaagcggc
      361 tgctgggcac cgcgctggcg atccaggccg gcatcggcaa cctcggcgtt tcgatcgtgc
      421 agttcgtgac cccgtggatc atcggcgtgg cgttgctggg cggcgccgcg ttcatgggcg
      481 acagccagac cctggtcaag aacggcgtgg agagccaggt ccacctgcag aacgcggcgg
      541 cggtgttcat cccgttcgtg gtggtgttcg gcatcaccgc ctggctgctg ctgaagtcgg
      601 tgccggtcaa ggccaacttc ggcgagcagt tcgacatctt caagtcgcgc cacacgtggt
      661 cgatgacctc gctgtacatg atgacgttcg gcggcttctc cggcctcgcc gcgaccttcc
      721 cgctgctgat caagcagggc ttcggcggct tcgaaggcgc gccggacccg ctcaagtggg
      781 cgttctgggg accgctggtg gggtcgatcg cgcgggtcat cgccggtccg ctgtcggaca
      841 agctgggcgg cgcgcgggtg acccaatggg ccggcatcgg catggtcgcg tgcacggtga
      901 tggtggcgat gaacgtctcg ccggactcgc tggacgaatt cccgaccttc gtcggctgga
      961 tgctggcgct gttcttcttc gccggcatcg gcaacgcgtc caccttcaag cagatgccga
     1021 tgctgttcgc gccgcgccag gccgccgggg tgatcggctt caccgccgcg atcgcggcct
     1081 acggcccgtt cctgttcggc atgctgttcg cgtgggcgtt cggcgcgttc aaggccgcca
     1141 ccccggtgtt ctacggcctg gccgtgttct tcgccttcaa cgtggtgctg aactggtgga
     1201 tgtatgcccg ccgcggcgcc gccaacccct gctgagaccc aggagaccgc ccccatgagc
     1261 gcagttcccg cccttccgtc ccgcggccag cagaacctgg ccctgtgggg cagcacgttc
     1321 gccttcaccg tgtgcttcgc ggtgtggacg atcttttcga tcatcggcat ccagatcaag
     1381 aaggacctgg ggctgaacga cacccagttc ggcctgctgg tggcgacccc gatcctcacc
     1441 ggctcgctga tccggctgtt cctgggcgtg tgggcggaac agtacggcgg ccgcatcgtg
     1501 ttcagcctgg tgatgctggc cggcgccagc gccacctgga tgctgaccta cgcgcagacc
     1561 tacccgcagt tcctgctggc ggcgctggcg gtgggcatcg ccggaggctc gttcgcggtc
     1621 ggcgtcgcct acgtctccaa gtggttcccg caggagaagc agggcaccgc gctgggcatc
     1681 ttcggcgccg gcaacatcgg ctcggcggtg accaagctgc tggcgccgat ggtgatggtg
     1741 gcgtacggct ggcacggtgt tgcgcgcttc tgggcgatcg ggcttgcgat caccgcggtg
     1801 ctgttcttcc tgttcaccaa ggacgacccg ggactggccc ggcgcaaggc cgaaggcatc
     1861 aagccggtgc cgttcgcgga gcagatgaag ccgctgcgca acctgcaggt gtggcgcttc
     1921 gcgctgtact acttcttcgt gttcggcgcg ttcgtggcgc tggcgctgtg gctgccgcgc
     1981 tacctgaccg gcgcctacgg cctggacgtg cgcaccgccg gcctgatcgc ggcctgctac
     2041 tcgatcccgg ccagcctgtt ccggatcgtc ggtggctggc tgtcggaccg gatcggcgcg
     2101 cgccgggtga tgtactggac cttcggcgtg tccgcgctgt gcacgttcct gctttcgtat
     2161 ccctccacca cctacgtggt cgacggcatc aacgggccgg tggaattccg ggtggcgatg
     2221 ggcctgctgc cgttcgtggt gctggtgttc gtgctcggct tcttcatgtc gctgggcaag
     2281 gccgcggtgt acaagcacat cccggtctac tacccgaaca acgtcggcgc ggtcggcggc
     2341 gtggtcggca tgatcggcgg ccttggcggc ttcatcctgc cgatcacctt cggcgcgatg
     2401 aacgatgcgc tggggatctg gaccagctgc ttcatgctgc tgttcgtgct ggtcagcacc
     2461 gcgctggtgt ggatgcactt cgcgatccgg cgcatggacg tgtcgcgcca cccgcagatc
     2521 accagcgaca ccgacctgcc cgagatcatg ccgcaggcgg gtcgctgagg ccgcgatgca
     2581 gggcccgcag ctcgacgcgg aacaggccct cgcgcggttg cgcgagggca accgccgctt
     2641 cgtcgaccat gtgatcagcc tggaggcgct gctcagccac gcgcggcgcg aggaccatgc
     2701 gcgcgcgcaa cgcccgttcg cggtggtgct gggctgctcc gactcgcgcg cgccggccga
     2761 attcgtgttc gaccagggcc tgggcgacct gttcgtgatc cgcgtcgccg gcaacatcgc
     2821 cgcgccgtcg caggtcggca gcatcgagtt cgcggtggaa cagttccagg tgcggctggt
     2881 ggtggtgctt ggccattcca actgcggcgc ggtgcgggcg accctggccc acctgcaggc
     2941 accgggcgac accagcccgg cgctgcgcgc gatcgtcgac cgcatcgcac ccgggatcca
     3001 gccggtgctg tccggctgcg gcggcgaatc ggacgcgtgc atgccgcagg cgatccgcgc
     3061 caacgtcgag gcgaccatcc gcaacctgcg cgcgcaatcg gactacctgc gctacgcgga
     3121 gttgcagggc ggcctgcgca tcgtcggcgc cgactacgac ctcagcagtg gcgcggtgga
     3181 cttcttcgcc agccaagcgc cggcctggac gaacgacgcc tgaccgcgcc cgcggggggc
     3241 gcgctcagcc gcgcgccagt tcgaccaggc ggccgtcgcg atcgaccgcc aggaagcgcg
     3301 cgatccgccc gcccttgacc tgctccacca tcatctgcag cggctgcacc gcttcgtcgg
     3361 aattgggggg atggccaccg cggccgtcca tcgccgcgac gaacaggatc cgccagtccg
     3421 ggatcgccgc cttcgattcc tcgagcaggg ccgcgaacga ggcgatctcc tccggcagct
     3481 tgtccacgca caccgccggc gccagcgccc cgccctcgcc gcgctcgaac gccgcgcgct
     3541 ccgccgcggt ggcgtcgggc gggcactcgg cgcgggcaaa caccagcagc agccgttgcg
     3601 gttcgggttg gccgcccgcg gcggcgagga aatcgtcgaa atgttgcatg cgtgggcagg
     3661 gttccgtggg gcgcccagtc taagccttcg ccatgcctgc aggcttgacc tgaatcagtg
     3721 caggcgccgc ggcgcatgcc gacactggcg tcccgatccg catccccgcc gagcccgcgc
     3781 catgaccatg cccgccaccc attcgcacgc ccccgatccc atccaggcac tgcgcgaact
     3841 gatcgcgggc ctggatcccg aatgcgaggt gctggtggac gaaatcagcg gcgatgtgct
     3901 ggtccgcggc agcatcgatc cggcccagct ggatgccgcg atcaaggcct cgggcctggg
     3961 gatgcgcatc gccgacagcg cgggcgaaag cggtggcggt tgctgcggcg gctgcggttg
     4021 cgcctgaccc ggcgaggctg cccatggccg cgcatgccct gaccgcgaag ccgtcgccat
     4081 gaccaccgcg gccagcgacg ggtcgctcgc gcgcctgcgc cgcaacctgc ggctgggtgg
     4141 cggctggtac gcgctgtacc cgccgccgcg cgccttccag gccttcggcg acgaccgctt
     4201 catcgccgcg atccgcgaga gcaacggcga tccgatcccg ccgcagctgg cgctgcattt
     4261 cgacatcccg gccgcctacc gcagcagttt ctgccacccc gacagccgcc cgcgcggcgc
     4321 ggacggcagc cgcgcggaag cgtaccggga ccggctggtg cgcgaggtcg ggctggtcgg
     4381 cagcctgttc gaccgcgacc gcgacgtggt ccaggtcagc ctggcgccgg ggatgacgcg
     4441 ctggatgagc gtggcccagg tcgccgaatt gctggcgagc ctgcgccgcc acttccacgc
     4501 ggaccgcgcc gccgacatcg acctggcgat gaccctggac ccggacgacc tggcctcggc
     4561 gccgctgcgc gactgggccg cgctgggctt caaccgggtc gggatcaaca tcgcggcgct
     4621 tgccggcgcc ggcgcggcgc cgtcgcggca acgcgaagac gtcgcccggc tggtcgcgca
     4681 ggcgcgcgag gccgggttcg ccaacctgcg ggtggaagtg ccgtacgggc tggccgggca
     4741 aaccgacgcc gcgttcgacg cgctggtggc ggcggtggcg gcggcccggc ccgaacgcgt
     4801 cggcctgcgc cactgtcctt cgcaggacgc gcccgatgcc ggcgcgaccc cggccgggca
     4861 cgacgcccac gcgcgcatgc tgctggcggg cgccgatgcg ctggagcacg cgggctacct
     4921 gcacgtcggc gtcgacctgt tcgcgcaggc ggacgatccg ctggtgcggg cccagcgcca
     4981 gggccgcatc catcgcgatg cgctcgggtt cggcgcgcac gccgccaccc acctggtcgg
     5041 cttcggggtg ggcgcgatca gccagctcgg cggttgccat gcgcagaacc cgctggacca
     5101 gccgagctgg gaggcacgca tcgaccgcgg ccaccgcgcc tgcaatcgcg gggtcgagct
     5161 cagcgaggac gaccacctgc gcgccgcggt cctgcaggac atcctgtgct acggacggat
     5221 cggggtggcc cagctcgagg cgcaccaccg gatcccgttc cgcgcgtact ttggcgacgc
     5281 ccttgcgcgc ctgcagccgc tgttcgagga cggctcgctg tgctgggacg gcgacgcgat
     5341 cctgctcgac cgcatcgccc ggcttgccgc gcaggcgatc gccagccagt tcgacccgct
     5401 gaccggggcc accgcaggcg gcaccgcgtg agcttccacg acctgcgcgg attcctggcc
     5461 gcgctggaac gcaatggccg cctgcgccgc gtgcgcgagc cggtggaccc ggaactggaa
     5521 accacctcgc tcagcctgcg cgcgttgaag gctggcgggc cggcgctgct gatggagcaa
     5581 ccgctgggcg cgcgccaccc atacctgggc aacctgttcg gccaccgcga ccgcatcgag
     5641 gcggcgctgg cggggcggcc gctggcatcc ctgcgcgaac tcggcgaact gctggcctcg
     5701 atcaaggagc ccaaatggcc gtccagcctg cgcgaggcgc tgggcacctg gccggagctg
     5761 gcgcagcttg cgcacgtggc accgcagcgg gtgcgcgaag ccgcgttcca gcacgaggtg
     5821 ctggaaggcg acgaggtcga cctgtcgcgc ctgccgatcc agcgctgctg gccggaggat
     5881 gcggcgaagc tgatcaccct tggcctggtg gtcacccgcg gcacccgcca gaagcgccag
     5941 aacgtggcga tctaccggca gcaggtgatc gcgcgcaacc gcatcatcct gcgctggctg
     6001 ccgcaccgcg gcggcgcgct cgactacgcc agctggcagc acagccatcc cgaccagccg
     6061 ttcccgctgc tggtggcgat cggcgcggac ccggcaaccc tgcttgcagc ggtggcaccg
     6121 gttccggaca ccctttccga atacgaattc gccggcctgc tgcgcggcga gcgcacccgc
     6181 gtctggcaca gcgcggccac cggactggat gcaccggccg gctgcgagat cctgctggag
     6241 ggccatatcg cccccggcga caccgcgctg gaaggcccgt tcggcgacca caccggctac
     6301 tacaacgcgc aggaccactt cccggtgatg accatcaccc gcatgcacct gcgccgcgat
     6361 gcgatctacc agggcagcta catgggccgc gcgccgcacg acgaaccgtc ggtgatggcg
     6421 atggcactga acgacgtgtt cgtgccgatc ctgcgcaagg tgttcccgga gatcgtggac
     6481 ttctacctgc cgccggaagc gtgctcctac cggatcgcgg tggtcagcat ccgcaagcaa
     6541 taccccggcc acgcgcgccg gatcatgatg ggcatctggt cgtacctgcg ccagttcacc
     6601 tacaccaagt tcgtgatcgt caccgacgac gacatcgacg tgcgcgactg gtcgcaggtg
     6661 atctgggcgc tctcgacccg ggtcgacccg gcgcgcgaca gcgtgatcat cgacaagacc
     6721 ccgatcgact acctcgattt ctccagcccc tccccgaacc tcggttcgaa gctggggctg
     6781 gacgccacca acaagtggcc ggccgagacc acccgcacct ggagcaagcc gatcgcgctg
     6841 gacccggcgg tggagaagcg ggtcgacgcg ctctggtcgc agctgctgca ggcaccctga
     6901 gccgcgaccc tgcgcttact cctgcgcggg ctggtcccag cccgggtcgt cgaagcgctg
     6961 cggatactgc gccagcgcgt cctgcatcgc cgcgagttcg ggctggccga agcgtccttc
     7021 gcggaccgca ccggcaccgg cgaccaacgg cgccagcagg ccgtgcaggg aggcgtcggc
     7081 ctccggctcc agcttgcagt tggcgatcat gtcggcgacc gcggcatcca cctgcgccgc
     7141 cgcatccttc tgctgggcct cggtgaggtg gcccatcagg ccatgctgca gcgcttcgac
     7201 cgcactccgg atccgcgcca tgccggcgcg caggggcgca tcggtcgccc agcgctggcc
     7261 cgctggcggc agcggtgcgg catcgacggg cgcatctgca tgcgcggcat gggcggcatg
     7321 ggcatcgtgg ccggcgtcgg ccgccgccgg cggcgcctgc gcggccggtg ccgccgccgc
     7381 gggttccgcg ccgggcctgg gcgaacagcc ggtagccacc gccagcagga ccagcgcggc
     7441 catggcgggg cgccagcggg cgcgcggcgg aatcgtgggg acctgcaaca tgcaatcgct
     7501 cctcggggat gcggccgggt ggccggcgat ggccgccacc atcgcatgcg cgcaaccgcg
     7561 gcgacatgac cggggtcaat gacgccacgg ttggccggcc ctagcatggc cgccttgtcc
     7621 ttccgggaag cgaccatgca actggtgtgt cccgccggca acctgccggc cctgcaggcg
     7681 gcggtggatg ccggcgccaa cgcggtttac gcaggcttcc gcgaccagac caacgcgcgc
     7741 gccttccccg gcctcaactt ctccgacgac gagttgcgcg ccggcgtccg ctatgcgcat
     7801 gccaagggcg cgaaggtgta cctggcgctc aacacctacc ccgacagcgc gcgcctgaag
     7861 gactggcatg cggcggtgga caaggccgcc gcgatcggct gcgacgccat catcgccgcc
     7921 gagatggcgg tgctggacta cgccgcgcgc acccacccgg gcctgccgcg gcacctgtcg
     7981 gtgcagggca gtgccaccac tgcgccggcg ctgaaatacg cgtacgaacg cttcggcatc
     8041 agccgcgcgg tgctgccgcg ggtgctctcg ctgcagcagg tggagcggct gtgcgagcgc
     8101 tcgccggtgg cgctggaggt cttcgccttc ggcagcctgt gcatcatggt cgaaggccgc
     8161 tgccagctgt ccagctacgt caccggggcc tcgccgaacc gccacggcgt gtgctcgccg
     8221 gcgaagttcg tgcgctggga cgaacacacc gatggcagcc gcagcgtgcg cctcaacgac
     8281 gtgctgatcg accagttcca gccgcaggag ccggccggtt acccgaccgt gtgcaagggc
     8341 cgcttcgagg tcggcaacga gatcttccac gcgctggagg agccgaccag cctcaacacc
     8401 ctggaactgc tgccgcggct ccagcaggcg ggggtggtgg cgctcaagat cgagggccgg
     8461 caacgtggcg tggcctacgt cagctcggtc acccgcacct ggcgcaaggc gctggaccgg
     8521 ctggccgcgg acccggcgca ctggcagctg cagccggact ggcaggcgga actgtcgcgc
     8581 cacgccgagg gccaccagac cacgctgggc ccgtaccacc gggcctggca ttgaggacgc
     8641 acgcatgaaa ctgagcctgg gcccgctgca gtacttctgg ccgcgcgaac gcaccctcgc
     8701 cttctaccgc gaggcggcgg cgtggccgct cgacatcgtc tacctcggcg aaaccgtgtg
     8761 cagcaagcgc cgcgaactgc gcaccgcgga gtggatcgaa ttggctcagg aactggccgc
     8821 cgccggcaag gaggtggtgc tgtcctcgct gtcgctgatc gaggccgagt ccgaactcgg
     8881 ggtggtcgat cgcctcgccg cgcacggcgg cttcaaggtc gaggccaacg acctctctgc
     8941 ggtgcaggtc tgccgcgagc gcggcacccc gttcgtggcc ggcccgtcgc tgaacctgta
     9001 caaccaccgc gcgctggcgc tgctggtcga agacggcctg gtgcgctggg tgcccggggt
     9061 ggaacagggc gaaaccctgg tgcgcgagct caagcaggca ttcgaggccg atggcggcgc
     9121 gatgccggaa ctggaggtga tcggctgggg ccggctgccg cttgcgtatt cggcgcgctg
     9181 cttcaccgcg cgcgcgctgg acgtggccaa ggaccagtgc ggcttccgct gcatcgacta
     9241 cccggacgcg ttgccgctgg ccacccgcga aggccgcccg ttcctgcgca tcaacggcat
     9301 ccaggtgctg ggcgaggaac tcaccgacct cggcccggaa ctgccggcgc tgcgcgcgtt
     9361 gggggtggac atcctgcgcc tgtacccgca ggcggaaggc atggcggacc tggtgcgcca
     9421 cttcgaccag gcccgcgcag gcgacgccgc cgcggcccca ctcggccagc gcaacggtta
     9481 ctggcatggc gaacccggca tgcaggtcgc cgccgccggc tgagcccgct caggccgccg
     9541 cgaccggccc cgccatcgaa gcgtcgtcat cgcggtagtg cgcacccagg ctgcggcggc
     9601 gctgcagtgc cgccgccagc agcgcccgcg ccagcccggc ctgccagcct tcggccgcca
     9661 gtccttcgca ttcgtccagc gccgcctgca ggccggcatg gttgcgcacc gggcccatcg
     9721 ccgcccccat caacgcgcgc aaccgttgca gcgcgggcgg cggcaacgcc gggccaaggc
     9781 ccacccacgc cgcctcgcca ccacggttct ccgccccgga aacggcggcc agccattgcc
     9841 cgagccggcg accgaacacc acgccttcca gcagcgaatt gctggccagc cggttggcac
     9901 cgtggacgcc gttgcaggcc acttcgccgg cggcgaacag ccccggcagc gaggtgcggc
     9961 cgtcggcgtc ggtggccagc ccgcccatgt ggaaatgcgc ggccggggtc accgggatcg
    10021 gctgcaggcg cggatcgatc gcatgcgcca ggcaggccgc gagcacggtc gggaacgcgg
    10081 ttggccaggc cacgccgatg tggcgggcat ccagccacgc gccacccgcg tgcaccgccg
    10141 ccgcgacccg tcgcgccacg atgtcgcgcg gggcaaggtc gcccagcgga tgcacgccgg
    10201 ccatcagcgg ctggccgtcg cggtccagca ggcgcgcgcc ggcaccgcgc agggcctcgg
    10261 tgaccagcgg caggctgtgc aggccgggca ccgccaacgc ggtgggatgg aactgcacga
    10321 attcgaggtc gcgggccgcc gcgcccgccg ccaatgccag tgcgaggccg ccgccatcgg
    10381 catccgccgg gttgctggtg gccgcgaaca ggccgccgat gccgcccgtg gccagcacca
    10441 ccgcgcgtcc gtcgaagcgc tgcctgctgc catcgtcgag ggtcgcggac accccgacca
    10501 cctggccatc gcgcacggcc agcgcatcca cgtcgcagcc ggcgatgcgg cggatccgcg
    10561 gctgcgcgcg cgcggcggcc accagcgcgc gcatcaccgc ggcgccggtg ccgtcgccgc
    10621 ccgcgtgcgc gatccgcgcg cggtcgtggc cgccctcgcg gccgagttgc aggcggccgt
    10681 cggcatcgcg gtcgaagtcc acgccgatcg cctgcagcca cgcgatcgcg gccggcgcgg
    10741 acgcagtcag cagttccacc atggcgacgt cgttgtggtg gctgccggcc acgcaggtat
    10801 cgcgcgcgtg cgccgcgggg gtgtcgcccg cgcccagcgc ggcggcgatc ccgccctgcg
    10861 cgagggtgct ggccgcgccc gcggcgcccg gccgccgggt cagcagcagc accggcgccg
    10921 gtgcggcggc cagcgccgcg gacaggccgg cgatcccgcc gccgaccacg atgatcggcg
    10981 cgtcgccgct cacacccggt cgcggcggcc gaccgccagc atgcgttcca gcgcgcgccg
    11041 cgcgccggcg gcgatcccgg ccggcacctc caccacgtac ttgcgttcca gcaggctggc
    11101 gtggatgttc ggcagggtga tccgctgcat gtacgggcac aggttgcagg gcttgacgaa
    11161 ctcgatgccc gggaaccgcg acttcaggtt gtcggccatc gagcattcgg taatcagcgc
    11221 cacccgctcc ggcttttccc tttccagcca gctgcccatg gcggtggtgg aaccgacgaa
    11281 atcggcttcg gccagcacct ccggcgggca ctccggatgc gccaccacgc gcgcgtggaa
    11341 ctgctcgcgg gtgtgccgga cctgctgcgc ggtgaaggtc tcgtggacct cgcagctgcc
    11401 gtcccacagc accagccgga tcttcgtctg cgaggcgacg tgcgcgccga ggaagcggtc
    11461 cggcaggaag atcacggtgt ccaccccgaa ttcggcggcg accgcttcca ccacctcgga
    11521 ggcattggcg gaggtgcagc aggcgtcgga ctcggccttc accgcggcgg tggtgttgac
    11581 gtagctcacc accggcagcc cgggatggcg cgcgcgcagg gcacgcacgt cgtcggcggt
    11641 gatcgacgcg gccagcgagc agccggcctc caggtccggg atcagcacgg tccggtccgg
    11701 ggccaggatc ttggcggttt cggccatgaa gtggacgccg cacaggacga tggtgtcggc
    11761 ctcgcaggtg gccgcggcct gcgccagcgc cagcgagtcg ccggtgatgt cggccacccc
    11821 gtggaagatc tccggcgact ggtagctgtg cgccatgatc accgcgccgc gctcgcgctt
    11881 gagttgctgg atggcgtcga tccaggccag gtgcagcggc acctccaggc acggcaccag
    11941 ttgttcaagg cgggcaccca gcgcggcatg gcgctcggcg atctcggggg tccatcgcgg
    12001 acggaaagcg tcttggcggg tcatcggcgg gtacggatcg ggcgcggcgg tgccgcgcgg
    12061 gagttgagca ccgcgcgcgg ggtcaggcgc gggcgtggta ggcggcgcgg gccgcgtcgg
    12121 cgaggcgggc accgcggttg agggcgatgc gcagccccag cggcacctgt tcccacggca
    12181 ggcggtcaag cacgttgcgc gcggtcaggc cgagttcggt gtcgccggtc agcaccagcc
    12241 ggcgctggaa gaacagggta tcggcgtcct cgcggcgccc ggccagcagc agcaggtcgg
    12301 tggcagtgcc gcgcacggtg gcctcggcgg gtgcatccac caccaccagc cggttgccct
    12361 gcagttccac cacccagccg atgccgaggt cgctgacctc gatcccgagc cggcgcccgt
    12421 ccatgaactc gatcgcgccc tcggcgagtg gctgcgccag cgcctgcgcc atcgcgcgtt
    12481 cgatcacccg ccgccgcagc gacggcggca ccgcccgcag caacggggcc agccggcgcg
    12541 gtggcgggat caggcgcagc agcggattgg cggccggcag gggggattcg ggttgggcat
    12601 ccatggtggc cgccatcatg cgccccgccg ccgcgggcgg gccttgatcc aggtcagatg
    12661 ccagtgcctg cgcgcggcag gcctcgccga tggcgaggcc gacgacgccg cttgcgacct
    12721 caggggccac cccgacccgc accgccagcg cggccgcgcg ttcgcggacc tggcgctcgc
    12781 gcccgggatc gcgcccgcgg gcaccggcgt gcagcttcag ccgtcccgcc agctgcgcca
    12841 gccgcgcgcg cgccgccagc agcaggacca ggccatcgtc gatgccatcg atcgcagtgc
    12901 gtaccggcgt cagcccggga acaggtctgg tcacgtccac ctccactggt cgccgcaggc
    12961 gcggcgacgc ccgccatcat tctgcagcag cgcccacgcg cgcgctgatg caggtcaagc
    13021 cgggatcccg caccggcgtc gccgcggcct cgcgcttgcc ggccgggtca gtccggcagc
    13081 agcgcgcgga tcacctcgcc ggccagctcg accgggctgg cacggtccgc gtgcaggtgc
    13141 agttcgggat gctcgggggc ttcgtacggc gaatcgatgc cggtgaagtt gcgcaactgg
    13201 ccggcgcgcg ccttcgcata cagccccttc acgtcgcgtt gctcggccac cgccagcggg
    13261 gtgtcgacga acacctccag gaactcgccg ggttcgaaca gcgcgcgcgc catctcgcgt
    13321 tcggcgcgga acgggctgat gaagctgacc agcacgatca ggccggcgtc ggtcatcagc
    13381 ttggccactt cggccacccg gcggatgttc tcgacccggt cctcgtcggt gaagccgagg
    13441 tccttgttca gaccgtggcg gacgttgtcg ccatccagca ggtaggtgtg ctggcccatc
    13501 gccagcagcc gcttctcgac caggttggcg atggtcgact tgccggcgcc ggacaggccg
    13561 gtgaaccaca ggcagcgcgg cgactggtgc ttgatccgcg cgcgcgccgc cttgtccacg
    13621 tccaggtgct gccagtggat gttgccggcg cgccgcaacg cgaagtccag cgttccggcg
    13681 gcgacggtgg cgttgctctg gcggtcgatc aggatgaagg cgccgagcgc acggttgcgc
    13741 gcgtacggct cgaacgcgag cggctggtcg aggtgcaggt tgcagcaggc gacctcgttg
    13801 agctccaggt gcttggcggc caggtgctcc tgggtgttga cgtcgacctt gtgcttgatc
    13861 tcggtgaccg aggcgccgac cgtgcgcgtg cccagcttca gcaggtaggg ccggcccggc
    13921 agcaggcgct cgtcgcccat ccacagcagg tgcgcggcga actggtcggc gacctccggc
    13981 ggatcgatcg cggcggcgat cacgtcgccg cggctggcat cgacctcgtc ggccagggtc
    14041 agggtgaccg cctggccgtc gccggcgtgc gcaaggtcgc cgccggcagt gacgatccgc
    14101 gccaccgtcg agcgccgtgc cgaaggcagc accaccactt cgtcgccgac cgcgacccgg
    14161 ccaccggcaa cggtgccggc gaagccgcgg aaatcctggt tgggacgatt gacccactgc
    14221 accggcaggc ggaatccggt ggcctgggtg gccggctcca cctgcacggt ttccaggtgc
    14281 tgcaacaggc tgggaccgcg ataccacggc atcgccgcgg acccgtcgac catgttgtcg
    14341 ccgcgcagcg ccgacagcgg gatcgcctgc acgttggcga tgccgagctg cgtggcgagg
    14401 tcgcggtagc cggcgacgat ggaatcgaac acgtcctgcc ggtaatccac caggtccatc
    14461 ttgttcaccg ccagcagaac gtggcggatg ccgagcagcg aggcgatgta gctgtgccgg
    14521 cgggtctggg tcagcaggcc cttgcgcgca tccaccagca ccaccgccag gtccgcggtg
    14581 gatgcgccgg tggccatgtt gcgggtgtac tgctcgtggc cggggcagtc ggcgacgatg
    14641 aacttgcgcc gttcggtgcc gaagaagcga tacgcgacgt cgatggtgat gccctgctcc
    14701 cgctccgcgg ccaggccatc gaccagcagc gcgaaatcga gctcgccgcc ctgggtgccg
    14761 tgcctgcggc tgtccgcttc cagcgcggcg acctggtcgt ccagcagcag tttggtgtcg
    14821 tgcagcaggc gcccgatcag ggtgctcttg ccgtcgtcca cgctgccgca ggtgatgaac
    14881 cgcagcaggc ccttggattc gtgctgttgc aggtaggcgg cgatgtccgg ggccatcaga
    14941 agtacccctc ctgcttcttc ttttccatcg atgcggcggg atcgtggtcg atcacccggc
    15001 cctgccgttc cgacgacgtg gccaccagca tctcggcgat gaccttttcc agcgtgtccg
    15061 ccccggattc gatcgcgccg gtcagcgggt agcagcccag ggtgcggaag cgcacgcgct
    15121 tcaacgccgg cacttcgtcc gcgcgcagcg gcaggcgctc gtcgtcgacc aggatcagcg
    15181 cgccgtcgcg ttccaccacc gggcgctcgc gcgccaggta cagcgagggc accgggatgt
    15241 tttcgcggta gatgtacagc cagacgtcga gttcggtcca gttgctgatc gggaacaccc
    15301 gcaccgactc gccggcatgg atccgggtgt tgtacaggtt ccacaactcg gggcgctggt
    15361 tcttcgggtc ccagcggtgc tggtcgttgc ggaagctgaa gatgcgttcc ttcgcgcgcg
    15421 acttctcctc gtcgcggcgc gcgccaccaa tcgccgcatc gaagccccac ttgtcgagcg
    15481 cctgcttcag cccctgggtc ttcatcacgt cggtgtgcac ggttgcaccg tggctgatcg
    15541 ggccgatgcc gcgggccacg ccgtccgggt tggtgtgcac gcgcagttcg atgccggtct
    15601 cggcggcgcg gcggtcgcgg aattcgatca tctcgcggaa cttccagccg gtatccacgt
    15661 gcagcagcgg gatcggcggg cgcgcgggat gaaacgcctt cgccaacagg tgcagcagca
    15721 ccgagctgtc cttgcccacg gaatacagca tcaccgggtt gcggaatccg gccgcgacct
    15781 cacggaggat atggatgctt tcggcttcca gccggtcgag gtgggagggt gctgcgtcca
    15841 aggcgaaagg tgcgttcttc gaagatcgcg caggatatca gtaggccttc ccggaccgca
    15901 gtcgaagcac attctccggc ggcatgaagg catcactgcg cgcggcttgc cagaagtcct
    15961 ggaacaccgc gtgcccgggg atgcggccgc ccagcaggtc gcccggctcg aggaagcggt
    16021 acagcgcccc cagcgagcgc acgtgggtcg gcgacacccg ccgcaggatg tgctccggcc
    16081 ccagctggtc cggatgctcc agcccggccg cgcacagcag gtcgcgcaat gcatgcaggg
    16141 tgttggcgtg gaactcggcg acccggctgg ccttgtccgg cacgtccagc cgcttccagc
    16201 gccccgggtc ctgggtggcg atcccggtcg ggcaacggtc ggtgtggcag gccatcgact
    16261 ggatgcagcc cagcgcgaac atgtagccgc gcgcggcgtt gcaccagtcc gcgcccatcg
    16321 ccagggtgcg cgcgatgtcg aatgcgccga cgatcttgcc ggcggcgccg atcctgatcc
    16381 ggtcgcgcag ttccagcccg accagggtgt tgtggaccag catcagcgcc tcgtgcatcg
    16441 gcaccccgac atggtcgatg aattccgccg gcgccgcgcc ggtgccgcct tcggcgccat
    16501 cgacgacgat gaagtcgggg aggatcccgc tctcgtgcat cgccttggcg atgccgaacc
    16561 attcccacgg atggccgatg gcgagcttga agccgaccgg cttgccgccg gacagctggc
    16621 gcaggcgggc gatgaagtgc agcaattcga gcggcgtgga aaacgcggaa tggcgggccg
    16681 gcgagacgca gtcacggccc atcggaacgc cgcgggtcac cgagatttcc gcgctgacct
    16741 tggccgccgg caacacgccg ccgtggccgg gcttcgcgcc ctgcgacagc ttcacttcga
    16801 tcatcttcac ctgcgcatcg cgcgcgttgg cggtgaagcg ctcctcgctg aagctggaat
    16861 cctcattgcg gcaaccgaag taacccgagc cgatctccca caccaggtcg ccgccggcct
    16921 cgcggtggta cggcgagatc gagccctcgc cggtgtcgtg gtagaagccg ccgcgcttcg
    16981 ctcccgcgtt cagcgcccgg atcgccgccg ccgacaacga gccgaagctc atcgccgaaa
    17041 tgttgaacac gctggccgaa tagggttgcg cgcactcagc gccgatcacg atgcggaaat
    17101 ccggggacgc gatcgccgcg ggcgccagcg aatggttgat ccattcgtag tcgatgccgt
    17161 agacgtcctg ctgggtgccg aacgggcgca cgtcgttgag gcgcttggcg cgctggtaga
    17221 ccagcgcccg ctgctggcgc gagaacggca cctcggcaag gtcggattcg atgaagtact
    17281 gccgcatctc cggcccgatc gattccagcc cgtagcggaa atgcgccagc agcgggtaaa
    17341 ggcggcgcag ggtgctcttg cgctgcaaca ggtcgatggt cccaagcgcg gccaatccgc
    17401 cgaacagcac cacccccgcc caccaacccg gcatgcgcag ggccagcagc agggatgcaa
    17461 gcgtcagcaa caggctggct gcgtaaacgg cgtagcggga cacggcggct ccatcgttgc
    17521 gctggtgccc ggagtcggga tcgaaccgac ataccctcgc gggtgaggga ttttaagtcc
    17581 cttgcgtcta ccagtttcgc catccgggct tgcgcggaag cttacgcgat gtatggctgc
    17641 ccggcccgcg gcgcgcggct gtgccggggt cacgccgcga gtacgtccat gcaggctaat
    17701 cggcggcgtc catgccgccg attgtccccg gcacagccgc gcgccacgac ccgggcaccg
    17761 acctccatcg cctcgcagca atatccggat cccgcaaaac aagaaacccc gccgaagcgg
    17821 ggtttcgtgt ctggaggccg aggtcggaat tgaaccggcg tacgcggatt tgcagtccgc
    17881 tgcata
//
LOCUS       DAORUK010000025        17742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_2837_length_17742_cov_16.688868, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000025 DAORUK010000000
VERSION     DAORUK010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 17742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17742
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2837_length_17742_cov_16.688868"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..821)
                     /gene="hisF"
                     /locus_tag="PK818_02980"
     CDS             complement(48..821)
                     /gene="hisF"
                     /locus_tag="PK818_02980"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HQY81730.1"
                     /translation="MLSRRIIPCLDVRDGRVVKGVRFRDHVDMGAIEDLALRYRDEGA
                     DELVFYDIGASPEARGVDVSWVRRVSDLLDIPFCVAGGIDSVERARAVLHAGADKVSV
                     NTPALLRPALIGELAAAFGSQCVVVGVDSLRDADGEWRVRSHSGDPSKMQAHARRTLD
                     WVVEAQQRGAGEIVLNCMGSDGVRAGYDIEQLQAVRETCAVPLVASGGAGSPDHFVAA
                     FRDADVDAALAASVFHSGAIAIGALKTLLHENGIEVRLG"
     gene            complement(815..1573)
                     /gene="hisA"
                     /locus_tag="PK818_02985"
     CDS             complement(815..1573)
                     /gene="hisA"
                     /locus_tag="PK818_02985"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HQY81731.1"
                     /translation="MTAPKAIRLYPAIDVREGRVVRLAQGDYARETRYPADPLAQAMA
                     YADAGVDALHLVDLDAAREGGYTLLPLLRAIAEDGRLQVQTGGGVRGDDDVLRILDAG
                     AARVVVGSLAVKETARVAGWLQQFGAERIVVALDTRCDDDGVWRLPVHGWTQGSGIEL
                     DALLDRYAAAGARHLLCTDIARDGMLGGPNFALYQRITQGWPAFAVQASGGVREAADV
                     AAARNAGCEGIVLGRALLEGRFTLADALREVAAC"
     gene            complement(1570..2172)
                     /gene="hisH"
                     /locus_tag="PK818_02990"
     CDS             complement(1570..2172)
                     /gene="hisH"
                     /locus_tag="PK818_02990"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020851325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HQY81732.1"
                     /translation="MTDLVIIDAGGANLGSVRAAFARLGVAPELSRDPARIATAGRLV
                     LPGVGAAAPVMDTLRASGIDVLLRGLKTPLLGVCIGMQVLFERSEEGDVAGLGLLGGQ
                     VRKLPASPGIRMPHMGWNRLARRVASPLLDGIDEGAQAYFVHSYGVVGSADAIAESEH
                     GSTFCAAVARGNIAGAQFHPERSGAVGARFLRNFLSWNPA"
     gene            complement(2183..3265)
                     /gene="hisB"
                     /locus_tag="PK818_02995"
     CDS             complement(2183..3265)
                     /gene="hisB"
                     /locus_tag="PK818_02995"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HQY81733.1"
                     /translation="MSAPVPILFVDRDGTLITEPDDFQIDAFEKLRFVDGVIPAMLRL
                     RDAGWQFVMVTNQDGLGSDAFPQAAFEGPHALMLQVFASQGITFREVLVDTSFERDNS
                     PNRKPNTGLVTGLLRDRGVDWTRSAMVGDRETDIRFAENLGIRGFKLRSPQFGDGTDW
                     AAIAHALVAAPRVANVVRKTNETAIEVAVDLDDAAKPDIATGLGFFDHMLEQLGTHGG
                     FALRLRCAGDLHIDEHHTVEDCALALGQALREALGNKRGIGRYGFVLPMDETQASATL
                     DLSGRPYFLFEGSFARERVGDLPTELVPHFFQSLCQSAGITLHLAVRGDNAHHQVEAC
                     FKVVARALRIALRREGDALPSSKGVL"
     gene            complement(3262..4356)
                     /gene="hisC"
                     /locus_tag="PK818_03000"
     CDS             complement(3262..4356)
                     /gene="hisC"
                     /locus_tag="PK818_03000"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQY81734.1"
                     /translation="MNGVSNTARILLRDDLRDFAGYRSARSEALQGSVWLNANESPSA
                     SPGDADGRLRRYPDPQPPALRDALAALYGLAPAQVLATRGSDEGIDLLLRAFCVPGRG
                     AIVIAPPVFGMYAVCARLHGARVVEVPARDAGDGWRCDLAAMADAALRQHANLVFVCN
                     PGNPTGEAIADADIALLAERLRGRALVVVDAAYAEFAGHDASAALLAAHENIVVLRTL
                     SKAHALAGARIGCVLAASELVGMLQRCQAPYPLAQPAVQAALAALAPDAVARTRTSVA
                     RVRGERERLLGELQSAPGVRRAYPSQGNFLLLRFDDAQAAFDALLAAGVVVRDMRATP
                     GLADALRISIGTPEQNQRVLASLAVARQAA"
     gene            complement(4353..5651)
                     /gene="hisD"
                     /locus_tag="PK818_03005"
     CDS             complement(4353..5651)
                     /gene="hisD"
                     /locus_tag="PK818_03005"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HQY81735.1"
                     /translation="MRRLDWNTLDADTQAQLLTRPTRALAAGVRGRVEEVMADVAARG
                     DVALRVLTEHYDGVRLEAFEVGPEEVRAAEASLAPGLRAAIDEAASRIDAFHRAGMPQ
                     PYGLETAPGVLCERILRPISPVGLYVPAGSAPLPSTALMLAIPAQLAGCPEIVLCTPP
                     RRDGSADPAVLVAAQYAPNARIFKLGGAQAIAAMSLGSESVPRCAKLFGPGNAYVDEA
                     KRQAAERPDGPAIDMPAGPSEVLVIADAGANPVFVAADLLSQAEHGPDSQVILLSDDD
                     ALLAAVAVEVERQLATLPRAEIARRALQHSRLVRVADIAQAIALSNDYAPEHLILGVR
                     EPRAWLDAVRDAGTVFMGDWTPESLGDYCSGSNHVLPTGGAARAWSGLSVSSFMKSLT
                     VQSASRAGIGAIGPCAVTLAEAEGLHAHARAVQLRLAVPR"
     gene            complement(5663..6577)
                     /gene="hisG"
                     /locus_tag="PK818_03010"
     CDS             complement(5663..6577)
                     /gene="hisG"
                     /locus_tag="PK818_03010"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HQY81736.1"
                     /translation="MSPKPAPAGRDRLRIAIQKNGRLSEPARALLGSCGLSWRESPDK
                     LFCYGDNAPVDLLLVRDDDIPGLIDSGLCDLGIVGRNVLVEQASERDETGKPANFREW
                     RALGFGGCRLSLAVPNEWDWRGPAQLQDRRIATSYPALTRAWLREQGVQAEVLVLSGS
                     VEIAPRLGQADVVCDLVSSGATLSANQLKPVCDLLQSEAVLAGPVADLDPLLAGTAQM
                     LLRRIDGVMRIRDSKLLMFQAPRGSVADVLRLLPDAEPATSYAVDGGDLLAMQALCHG
                     VVTWQRLEELKRAGARGLMVLPVERMLA"
     gene            complement(6588..6902)
                     /locus_tag="PK818_03015"
     CDS             complement(6588..6902)
                     /locus_tag="PK818_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HQY81737.1"
                     /translation="MKSRPYNPPKREADADFRQLARALAALDDPRQVAAFLRDLCTPA
                     ELEALCDRWKVVPLIVAGEPYRGIHERTGVSVTTIGRVARTLEHGAGGYAAALAAPTP
                     SH"
     gene            complement(7071..7982)
                     /locus_tag="PK818_03020"
     CDS             complement(7071..7982)
                     /locus_tag="PK818_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQY81738.1"
                     /translation="MNQDPFGNRNPQSRRRGAGGLRLWVLVLFAGYAAYYWYSNRTVD
                     PYTGEEVMIDKGLSAEDESAMGLQAYREILGQERAVDPDAQVARQIRAIAERLISRVD
                     EVEADLAAEKGVQPRNFAGNFEWQVSVIESGQANAFALPGGKMAVYTGLVPIARNADG
                     MAVVMGHEIAHALMRHGAQRMARGKLEQIGQMAGAASGMDAGTLQTVMSAYGVTSALP
                     YARGHESQADEVGLMLAAAACFDPAEAMPLWERMAQLGGGQSPPEFASTHPSAESRIA
                     HLQSLQQKAMGFRQKYCGQGGGAAAAG"
     gene            complement(8036..9868)
                     /locus_tag="PK818_03025"
     CDS             complement(8036..9868)
                     /locus_tag="PK818_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019336851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HQY81739.1"
                     /translation="MRAGWPLCFFVTIALAGCGPQAPVPAAQPAAPERTGSVRISGGD
                     ELAQVLSWSLPQVQLQASDAVQARARARRALAAGDLFESADSAIPLLLALQRLQPDDG
                     GIAQALDAAREALVAQGDAALEAGDEDLDGLRAAQRRGAVLRRLWPQEAVVVAYLQRV
                     DRSERAWDRNAEGEALLASGSLGEGGGGALAAFRAALQLRPGQARALQGLAAVESALI
                     RRAEAAALQADFDAAHAWLAHAERVRRHPGTISTARHRIEVVRAERVRRLRDEGLIAL
                     GNDNGLRLARERLAEMLRIAMPGDPASLELRQRIDLATHYGLFRPGQVFTDGLRDGSR
                     GPVLVVVPHGAFRMGAPEGEHDATAAERPQHVVRLERGFAMSRNEVSVGEFGRFVVAT
                     GYQPRATQRGHSMAYDVRSGNFVRGSGIDWRSGHDGRPASADMPVIHVSARDAEAYTA
                     WLSEQAGAHYRLPSEAEFEYALRAGSSTRFPWGEGRPPAGSENLTGALDASPQGRRWS
                     NAFAGYGDGWWGPAPVGTLAANGFGLHDIAGNVSEWVADCWHAGYRRAPANGSAWVNP
                     GCRDRMYRGGSWASAPAQVRSAWRASGGTDVTNARVGFRVVRQL"
     gene            9942..11066
                     /gene="rnd"
                     /locus_tag="PK818_03030"
     CDS             9942..11066
                     /gene="rnd"
                     /locus_tag="PK818_03030"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009591039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HQY81740.1"
                     /translation="MTHWIDTPDALRAHLAQPPASVGLDTEFIRERTYWPQLALVQVA
                     LGEADPAAPDAHILLVDPLVPGMCEALVPLLADPAVLKIMHSASEDLVAFRHACGVLP
                     APLFDTQVAAALAGVGGGMGYQKLVQASTGVTLAKGETRSDWLRRPLSPAQLDYAADD
                     VRHLHALHRQLDAALQAQGRSGWLQEDVARLLATVADEDGDRWPHLSLRSAQFLDGDG
                     QRRLLRLLRWREAYARRSDRPRSWILDNELAVAIARQPALGRGGLQQLLDATPKAPRN
                     LGDALFEALSSPLADEADAPPARGDDRDKKQLRALQDAVAAASAELGLPEGLLASRRL
                     LESLQDGGGWTGALAGWRRAVLEPRLSPLLGAGGAAAASV"
     gene            11086..11161
                     /locus_tag="PK818_03035"
     tRNA            11086..11161
                     /locus_tag="PK818_03035"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11119..11121,aa:Ala,seq:cgc)
     gene            11298..12515
                     /locus_tag="PK818_03040"
     CDS             11298..12515
                     /locus_tag="PK818_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY81741.1"
                     /translation="MKRPLILVVVAGLAVWAGWTVLQSLRGPQLAGYQVAARPLVQTV
                     VASGRVVAVSRAQVGSPVTGVVLERRVREGDAVQPGDVLAVLRADDLQAALREAEAAV
                     AQLQQSTRPQAQAALREAEARLQQASREATRRRDLFKRQLIAREALEQALQAETIARS
                     AAEQARLSLNSLSAGNPNEAAARARAAAARAQLAKTIIRAEVAGTVLTRNAEPGDLVQ
                     PGRVLFEIARAGATEVLVPLDEKNLEVLAIGQAAQCVADAYPTRPFPAQVSFIAPSVD
                     PQRGSVDIRLTVSPVPAFLRQDMTVSVNVETGRRERAVVVPNDALGPVDGNRASLWRV
                     EDGRAVRRQVTLGLRGLTMAEVTDGLAAGDWILADAAAPLQEGDRVRVVAGALPADAT
                     DAATRKELPAKLD"
     gene            12515..13723
                     /locus_tag="PK818_03045"
     CDS             12515..13723
                     /locus_tag="PK818_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY81742.1"
                     /translation="MPMWIEWTIATRFLREGKSQSTLILVGIAVGVAVIVFLTALITG
                     LQGNIVNRTLGTQAHIKVQPTEEANRVLAPADGSVHLVLESKRAQRLRSINNWQQVRD
                     VLDALPRVTAVSPVISGPAFARRGEALESVALVGIDAARYQRIIPIADDIVAGRFRIG
                     AGEAVVGSQLARDLGLRVGDKLRLDGGQGRDSVVNVAGIFELGVRELDARYVYLDMKQ
                     AQSLLDLPGAATIIDVTVDDIFAAREVAARIARLTGLKAESWMETNAQLMNALRSQSL
                     STRMISVFVALSVALGIASVLSVSVVQRTREIGILRAMGTTRPQMLRVFLVQGALFGL
                     AGSLVGGVAGYGMVMAFNTFGPKLFYIPVDPWLLVAASVLATVTGIVSAAIPARRAAG
                     LDPVEAIRHV"
     gene            13716..14459
                     /locus_tag="PK818_03050"
     CDS             13716..14459
                     /locus_tag="PK818_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY81743.1"
                     /translation="MSDRDAGPDVLRLQGLRKSYNVGKPTEVEVLHGLDLRLQRHDFA
                     ALVGASGSGKSTLLNLVGLLDTPTAGELFLLGEATRDMDDARRTALRGGAIGFVFQFH
                     HLIQAFTALENVLMPLMVATGKPGREHIDRARALLAEVGLQGLEQRKPDQLSGGQQQR
                     VAVARALVTRPALLLADEPTGNLDSHSAAEVFELFRKFNREFGCAVLLVTHDPRLSEQ
                     CDRTVALVDGRIASDTAKAGKTEPRPTSA"
     gene            complement(14560..15684)
                     /locus_tag="PK818_03055"
     CDS             complement(14560..15684)
                     /locus_tag="PK818_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002802676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HQY81744.1"
                     /translation="MGTPRLFEPLAVGRLQLANRIVIAPMCQYSAVDGCMTDWHLIHL
                     GQLSHSGAALLTIEATAVLPEGRITDGDVGLWDDATEAAMAVVLQGVRRWSAMPVAIQ
                     LAHAGRKASCALPWHGGRQVAPGQARGWQTLAPSALPFNDHDHAPVAMTRKDIDGVTD
                     AFAAAARRAARLGIDAVQVHAAHGYLLHQFLSPLSNRRSDEYGGSLENRMRFPLQVFD
                     AVRDAFPAGRAVSVRVSATDWMPDGWDIEQAIAFAQALEARGCSAIHVSSGGLHPAQA
                     IAAGPGYQVPLARAVKQAVAMPVVAVGMITGFDHAEAIVANGDADLIAIARGILYDPH
                     WPWHAAAHLGASVAVPPQYLRSQPPQFPGLLAQGGGSAIA"
     gene            complement(15777..16100)
                     /locus_tag="PK818_03060"
     CDS             complement(15777..16100)
                     /locus_tag="PK818_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HQY81745.1"
                     /translation="MSENHDDGRRRFLGYAALVALAPLAANQAVAQTKALPKLPLDNA
                     QAKSLGYVEKAATSKHATFKPGRECSNCMLYKGTAGQAYGPCQLFPKNSVAAKGWCSA
                     WVKKA"
     gene            complement(16314..16973)
                     /locus_tag="PK818_03065"
     CDS             complement(16314..16973)
                     /locus_tag="PK818_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HQY81746.1"
                     /translation="MREAGMVAIRTVVPDIDQDIRYFGSDNFVGTRVDGYLAPHCWLK
                     REAAEALARVERRLRESHQRLRIFDCYRPTRAVAHFMRWANDPADLATKARHYPDFDK
                     PQLLDGYIAPVSGHSRGATVDLTLLQCDADDAACRAVDMGTDFDFFGTRANTDSAEVT
                     ALQRRNRDRLRAAMAAEGFRNYPMEWWHFTLQLEPTPGLLYDIPVDAPEPTKPDAATG
                     P"
     gene            complement(17068..>17742)
                     /locus_tag="PK818_03070"
     CDS             complement(17068..>17742)
                     /locus_tag="PK818_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQY81747.1"
                     /translation="RTPMPISAQRRRWDNTHFHAYAYGFRLADADGAWTVSHTGTLGG
                     MYSMMMLVPDRRSGFVLMINGDGDRARGVLGQVLLKLFTAPERSLGVAGYADELAKPA
                     TAAAGATPALPDIGARKPVAASALRDWLGIWRDPWFGDVRVCAVGDGVEWRSLKSPKM
                     HGKIGTLDGRHVLRWDDIAVDEHAWLDFSGSGDARQLRMAKLNPDGDFSSDYEHLAFA
                     WVGRCD"
BASE COUNT         2557 a         6511 c         6208 g         2466 t
ORIGIN      
        1 caccctgctt gtcccagtcc agcgtgtcga tgtcgaacag tgtggaatca gccaagacgc
       61 acctcgatgc cgttctcgtg caatagggtt ttgagcgcgc cgatcgcgat cgcgccggag
      121 tggaacacgc tggccgcgag cgcggcgtcg acgtcggcat cacggaaggc ggcgacgaag
      181 tggtcggggc tgccggcccc gccggaggcc accagcggca ccgcgcaggt ctcgcgcacc
      241 gcctgcagct gttcgatgtc gtagccggcg cgcacgccat cgctgcccat gcagttgagc
      301 acgatctcgc cggcgccgcg ctgctgcgcc tcgaccaccc aatcgagggt acggcgggcg
      361 tgcgcctgca tcttcgaagg atcgccgctg tggctgcgca cccgccattc gccatcggcg
      421 tcacgcaggg aatcgacccc gaccaccacg cactggctgc cgaacgccgc cgccagctcg
      481 ccgatcagcg ccggccgcag caatgccggg gtattgaccg agaccttgtc ggcaccggca
      541 tgcagcaccg cgcgcgcacg ttcgacgctg tcgatgccgc cggcaacgca gaacgggatg
      601 tccagcaggt cggacacgcg cctgacccat gacacgtcga cgccacgcgc ttccgggctg
      661 gcgccgatgt cgtagaacac cagctcgtcg gcgccctcgt cgcggtagcg cagggccagg
      721 tcctcgatcg cgcccatgtc gacgtggtcg cggaagcgca cgcccttgac cacgcgcccg
      781 tcgcgcacgt ccaggcatgg aatgatgcgg cggctcagca tgcggccacc tcgcgcagcg
      841 catcggccag ggtgaagcgg ccctccagca gggcgcgacc gaggacgatg ccttcgcaac
      901 cggcattgcg cgccgccgcg acatccgccg cctcgcgcac gccaccggag gcctgcacgg
      961 cgaacgccgg ccagccctgg gtgatgcgct ggtacagcgc gaaattgggc ccaccgagca
     1021 tgccgtcgcg ggcgatgtcg gtgcacagca ggtggcgcgc gccagccgcg gcatagcgat
     1081 ccagcaaggc atcgagttcg atgccgctgc cctgggtcca gccatgcacc ggcaggcgcc
     1141 acacaccatc gtcgtcgcaa cgcgtgtcca gcgcgaccac gatgcgctcc gcgccgaact
     1201 gctgcagcca gccggccacg cgcgcggtct ccttcaccgc cagcgaaccg accaccaccc
     1261 gcgccgcgcc ggcatccagg atgcgcagga catcgtcgtc gccacgcacg ccgccgccgg
     1321 tctgcacctg caggcgtccg tcttccgcga tcgcacgcag caggggcaac agcgtgtacc
     1381 cgccttcgcg cgcggcatcc aggtcgacca ggtgcagcgc atccacgccg gcatcggcat
     1441 aggccatcgc ctgggcgagc ggatccgccg ggtaacgggt ttcgcgtgcg tagtcgcctt
     1501 gcgccagccg caccacgcgg ccctcgcgca cgtcgatcgc cgggtacagg cgaatggcct
     1561 ttggcgcggt catgccggat tccaggacag gaagttgcgc aggaagcgcg cgccgaccgc
     1621 gccggagcgc tcgggatgga actgcgcgcc agcgatgttg ccgcgcgcga ccgcggcgca
     1681 gaaggtgctg ccgtgttcgc tctcggcgat cgcatccgcg ctgccgacca cgccatagct
     1741 gtgcacgaag taggcttgcg cgccctcgtc gatgccgtcc agcagcggcg aggcaacgcg
     1801 cctggcgagg cggttccagc ccatgtgcgg catgcggatg cccggcgatg ccggcaactt
     1861 gcggacctgc ccgccaagca ggccaaggcc ggccacgtcg ccctcctcgc tgcgttcgaa
     1921 caagacctgc atgccgatgc agacgccgag caaaggcgtc ttcaagccgc gcagcaggac
     1981 gtcgatgccg ctggcgcgca gggtgtccat caccggcgcg gcagccccca cgccgggcag
     2041 caccagccgc ccggcagtgg cgatgcgcgc gggatcgcga ctcagttcgg gcgccacgcc
     2101 cagccgcgcg aatgcggcgc gcacggagcc gaggttggcg ccgccggcat cgatgatcac
     2161 caggtccgtc acgacatcac catcagagca cgcccttgct gctcggcaag gcatcgccct
     2221 cgcggcgcaa cgcgatccgc agcgcacgcg cgaccacctt gaagcaggct tcgacctggt
     2281 ggtgcgcgtt gtcgccgcgc accgccaggt gcagggtgat gccggcggac tggcacagcg
     2341 attggaagaa gtgcggcacc agttcggtcg gcaggtcgcc cacgcgttcg cgcgcgaagc
     2401 tgccttcgaa caggaagtac ggccgcccgg acaggtccag ggtggcgctg gcctgcgtct
     2461 cgtccatcgg cagcacgaaa ccgtagcggc cgatcccgcg cttgttgccc agcgcttcgc
     2521 gcagcgcctg tcccagcgca agcgcgcaat cctccaccgt gtggtgctcg tcgatgtgca
     2581 ggtctccggc gcagcgcagg cgcagggcga agccaccatg ggtgcccagc tgctccagca
     2641 tgtggtcgaa gaaacccagc ccggtggcga tgtccggctt tgcggcatcg tccaggtcga
     2701 cggccacctc gatggcggtc tcgttggtct tgcgcaccac gttcgcgacc cgcggcgccg
     2761 cgaccagcgc gtgcgcgatc gcggcccagt cggtgccatc gccgaactgc ggactgcgca
     2821 gcttgaagcc gcggatgccg aggttctccg cgaagcggat atcggtctcg cggtcgccga
     2881 ccatcgcgct gcgcgtccag tcgacgccgc ggtccctgag caggccggtc accaggccgg
     2941 tgttcggctt gcggttcggc gagttgtcgc gttcgaaact ggtatcgaca agcacctcgc
     3001 ggaaggtgat gccctggctc gcgaacacct gcagcatcag cgcgtgcggg ccttcgaagg
     3061 cggcctgcgg gaaggcgtcg ctgcccaagc cgtcctggtt ggtcaccatc acgaactgcc
     3121 agccggcgtc gcgcaggcgc agcatcgcgg ggatcacgcc gtccacgaag cgcagcttct
     3181 cgaaggcatc gatctggaaa tcgtccggct cggtgatcag ggtgccgtcg cggtccacga
     3241 acaggatggg cacgggcgcg ctcatgccgc ctgcctcgcg accgccagcg acgccagcac
     3301 ccgctggttc tgctccgggg tgccgatgct gatccgcaac gcgtccgcaa ggccgggcgt
     3361 cgcgcgcatg tcgcgcacca ccaccccggc agccagcagc gcgtcgaacg ccgcctgcgc
     3421 atcgtcgaag cgcagcagca ggaaattgcc ctgcgacgga tacgcccggc gcacgcccgg
     3481 cgcggattgc agctcgccca gcaagcgctc gcgctcgcca cgcacccgcg ccaccgacgt
     3541 ccgcgtgcgc gccaccgcgt ccggggcgag cgccgccagc gccgcctgca ccgccggttg
     3601 cgccagcgga tacggcgcct ggcagcgctg cagcatgccg accagctcgc ttgccgcgag
     3661 cacgcaaccg atccgcgcgc ccgccagcgc atgcgccttg gacagggtcc gcagcaccac
     3721 gatgttctcg tgcgcggcca gcagcgccgc acttgcatcg tgcccggcga actcggcata
     3781 ggccgcgtcc accaccacca gcgcacgtcc gcgcaggcgc tcggcgagca gtgcgatgtc
     3841 ggcatcggcg atcgcctcgc cggtgggatt gccggggttg cacacgaaca ccaggttcgc
     3901 gtgctggcgc agggcggcgt ccgccatcgc cgccaggtcg cagcgccagc catcgcctgc
     3961 atcccgcgcc ggcacttcga ccacgcgcgc gccatgcagc ctcgcgcata ccgcgtacat
     4021 cccgaacacc ggcggcgcga tcacgatcgc gccgcgcccg ggtacgcaga acgcgcgcag
     4081 caacaggtcg atgccttcgt cgctgccgcg ggtggccagc acttgcgcgg gcgccagccc
     4141 gtacagcgcc gccagcgcat cccgcagcgc cggtggctgc gggtccggat agcggcgcag
     4201 gcgaccgtcg gcatcaccgg gcgaggcact cggcgactca ttggcattca gccagaccga
     4261 gccctgcaac gcctcgctgc gcgccgaacg gtaaccggcg aagtcgcgca ggtcgtcgcg
     4321 cagcaggatc cgcgcggtat tcgacacgcc gttcatcgcg gaaccgccag ccgcagctgc
     4381 accgcccgcg cgtgcgcatg caggccttcg gcctcggcca gggtcaccgc gcacgggccg
     4441 atcgcgccga tgccggcgcg gctcgcgctc tgcacggtca gcgacttcat gaaactggac
     4501 accgacaacc cgctccacgc gcgcgccgcg ccgccggtgg gcagcacgtg gttgctgccg
     4561 gagcagtagt cgcccagcga ttccggggtc cagtcgccca tgaacacggt gccggcatcg
     4621 cgcaccgcgt ccagccatgc gcgcggctcg cgcacgccca ggatcaggtg ctcgggcgcg
     4681 tagtcgttgc tgagcgcgat cgcctgggcg atgtcggcga cccgcaccag gcgcgagtgc
     4741 tgcagcgcgc ggcgggcgat ctcggcgcgc ggcagggtcg cgagctggcg ttcgacctcg
     4801 accgccaccg ccgccagcag ggcgtcgtcg tcgctgagca ggatcacctg cgaatccggg
     4861 ccgtgttcgg cctgcgacaa caggtcggcg gcgacgaata ccgggttggc cccggcgtcg
     4921 gcgatcacca gcacttccga ggggccggcg ggcatgtcga tggccggacc gtccggccgt
     4981 tccgcagcct ggcgcttggc ttcgtcgacg taggcattcc ccgggccgaa cagcttggcg
     5041 cagcgcggca cgctctcgct gcccaggctc atcgcggcga tcgcctgggc gccaccgagc
     5101 ttgaagatgc gtgcattcgg ggcgtattgc gcggccacca gcaccgccgg gtcggcgctg
     5161 ccgtcgcggc gcggcggggt gcacagcacg atctccgggc aacccgcgag ctgggccggg
     5221 atcgccagca tcagcgcggt cgacggcagt ggcgcgctgc ccgccggcac gtacagcccg
     5281 accgggctga tcggccgcag gatgcgctcg cagagcacgc cgggcgcggt ttccagcccg
     5341 tatggctgcg gcatgccggc gcggtggaag gcatcgatcc gcgacgccgc ctcgtcgatc
     5401 gccgcgcgca agccgggcgc cagcgacgcc tccgcggcgc ggacctcttc ggggccgacc
     5461 tcgaacgcct ccaggcgcac gccgtcgtaa tgttcggtga gcacgcgcag ggccacgtcg
     5521 ccgcgtgcgg ccacgtcggc catcacttcc tcgacccgac cgcgcacgcc cgcggccagc
     5581 gcgcgcgtgg gccgggtcag caactgcgcc tgggtgtcgg catccagggt gttccagtcg
     5641 aggcgccgca tgaaatccgc gctcatgcca gcatcctctc gaccggcagc accatcaggc
     5701 cgcgcgcgcc ggcgcgcttg agttcctcca gccgctgcca ggtgaccacg ccgtggcaga
     5761 gcgcctgcat cgccagcagg tcgccgccat cgacggcata ggacgttgcc ggttccgcgt
     5821 ccggcagcag gcgcaggaca tccgcgaccg aaccacgcgg cgcctggaac atcagcagct
     5881 tgctgtcgcg gatgcgcatc acgccgtcga tccgccgcag cagcatctgc gcggtgcccg
     5941 ccagcaaggg atcgaggtcg gccacggggc cggccagcac cgcctcgctc tgcagcaggt
     6001 cgcacaccgg cttcaactgg ttggcggaca gggtggcgcc gctggagacc aggtcgcaga
     6061 ccacgtccgc ctgccccagc cgcggcgcga tttccaccga gccggacagc accagcactt
     6121 cggcctgcac gccctgctcc cgcaaccacg cgcgggtcag cgccgggtag gacgtggcga
     6181 tccgcctgtc ctgcaactgc gccggacccc gccagtccca ctcgttcggc accgccagcg
     6241 acagccggca gccgccgaag ccgagcgcgc gccattcgcg gaaattcgcc ggcttgccgg
     6301 tctcgtcgcg ctcgctggcc tgctcgacca gcacgttgcg gccgacgatg ccgagatcgc
     6361 acaggccact gtcgatcagg ccggggatgt cgtcgtcgcg caccagcagc aggtcgaccg
     6421 gggcgttgtc gccgtagcag aacagcttgt ccgggctttc gcgccaggac aggccgcaac
     6481 tgcccagcag cgcgcgcgca ggttcgctga ggcggccgtt cttctggatc gcgatgcgca
     6541 gtcgatcgcg cccggcgggg gcgggtttgg ggctcatggg ggcggtctca gtgcgagggg
     6601 gtgggtgcgg caagcgccgc ggcgtagccg ccggcgccgt gttcgagggt gcgggcgacc
     6661 cggccgatgg tggtgacgct gaccccggtg cgctcgtgga tgccgcggta gggctcgccg
     6721 gcgacgatca gcggcaccac cttccagcgg tcgcacagcg cctccagctc ggccggggtg
     6781 cacaggtcgc gcaggaacgc ggcgacctgg cgcgggtcgt cgagcgccgc cagcgcgcgc
     6841 gccagctgcc ggaaatcggc atcggcttcg cgcttgggcg ggttgtaggg ccggctcttc
     6901 atcgttgcca gattcgatca atgtattaac gtgctaatac attaaccgcc gctgccgggc
     6961 cgcgtcaagc accctgcatg cgcgccgccg ccgcccgcga cggaccggtg cctgcaaacg
     7021 gggaacgggc cctgcggccc gtccactggt gcctccgcgg gcgggaagcc tcagccggcc
     7081 gcggctgcgc cgccaccctg cccgcagtac ttctggcgga accccatcgc cttctgctgc
     7141 agcgactgca ggtgcgcgat gcggctttcc gcgctcgggt gggtggacgc gaattcgggc
     7201 gggctctggc cgccgcccag ctgcgccatg cgttcccaca gcggcatcgc ctcggccggg
     7261 tcgaagcagg ccgccgcggc cagcatcagc ccgacctcgt ccgcctgcga ctcgtggccg
     7321 cgcgcatacg gcagcgccga ggtcacgccg tacgcggaca tcaccgtctg cagggtgccg
     7381 gcatccatcc cgctggcggc accggccatc tgtccgatct gctccagctt gccgcgcgcc
     7441 atccgttgcg cgccatgccg catcagcgca tgcgcgatct cgtggcccat caccaccgcc
     7501 atgccgtcgg cgttgcgcgc gatcggcacc aatccggtgt agaccgccat cttgccgccc
     7561 ggcagcgcga atgcgttggc ctgcccggac tcgatcacgc tgacctgcca ttcgaaattg
     7621 ccggcgaagt tccgcggctg cacgcccttt tcggcggcga ggtcggcctc gacctcgtcg
     7681 acgcgcgaga tcaggcgctc ggcgatggca cggatctgcc gcgcgacctg cgcatccgga
     7741 tcgaccgcgc gctcctggcc gaggatctcg cggtaggcct gcaggcccat cgcgctttcg
     7801 tcctcggcgc tcaggccctt gtcgatcatc acctcctcgc cggtgtacgg gtcgacggtg
     7861 cggttggagt accagtagta cgcggcgtag ccggcgaaca gcaccagcac ccacagccgc
     7921 aggccaccgg cgccgcgacg acgcgattgc ggatttcgat ttccgaacgg atcctggttc
     7981 atcgcatgct cccgtctgct tgcgcggtac cccgcgcgcg tccttcgatc gtttgctaca
     8041 gctgccgcac cacccggaac ccgaccctgg cattggtgac gtcggttccg cccgatgccc
     8101 gccatgccga gcgcacctgc gccggcgcac ttgcccagga gccgccgcga tacatgcggt
     8161 cgcggcaacc cgggttgacc catgcgctgc cgttcgccgg cgcgcgccgg tacccggcgt
     8221 gccagcaatc ggccacccat tcgctgacat tgcccgcgat gtcgtgaagg ccaaacccgt
     8281 tcgccgccag cgtgccgacc ggcgccggcc cccaccagcc gtcgccgtaa cccgcgaacg
     8341 cgttgctcca gcgccgtccc tgcggcgacg cgtccagcgc gccggtgagg ttctcgctgc
     8401 ccgcgggcgg cctgccctcg ccccacggga agcgggtgct gctgccggcg cgcagcgcgt
     8461 actcgaattc cgcctcgctg ggcagccggt agtgggcccc ggcctgctcc gaaagccacg
     8521 ccgtgtatgc ctcggcgtcg cgcgcggaca cgtggatcac cggcatgtcc gcggaggccg
     8581 gccgcccgtc atgcccggag cgccagtcga tcccgctgcc gcgcacgaag ttgccgctgc
     8641 gcacgtcgta ggccatcgag tggccgcgct gggtggcacg cggctggtag ccggtggcca
     8701 cgacgaagcg gccgaattcg ccgacgctga cctcgttgcg cgacatcgcg aaaccgcgct
     8761 ccagccgcac cacgtgctgc ggccgctcgg cggcggtggc gtcgtgctcg ccctccgggg
     8821 cgcccatccg gaacgcgccg tgcggcacca ccaccagcac cggcccgcgg ctgccatcgc
     8881 gcaggccgtc ggtgaacacc tggccgggcc ggaacaggcc gtagtgggtc gcaaggtcga
     8941 tgcgctggcg cagctccagg ctggccggat cgccgggcat ggcgatgcgc agcatttcgg
     9001 ccaggcgctc gcgcgccagc cgcaggccgt tgtcgttgcc gagcgcgatc aggccctcgt
     9061 cgcgcagccg gcgcacgcgc tcggcgcgaa ccacctcgat ccggtggcgc gcggtggaga
     9121 tggtgccggg gtgccggcgc acgcgctccg catgcgcaag ccacgcatgc gcggcgtcga
     9181 aatcggcctg cagcgccgcc gcttccgcgc gccggatcag cgcgctctcg accgcggcaa
     9241 ggccctgcag cgcacgcgcc tgcccggggc gcagctgcag cgccgcacgg aacgccgcca
     9301 gtgcgccgcc gccaccttcg cccaggctgc cgctggccag cagggcctcg ccctcggcat
     9361 tgcgatccca cgcccgttcg ctgcgatcca cccgctgcag gtaggccacg accactgcct
     9421 cctgcggcca cagccggcgc aacaccgcac cacgccgctg cgccgcgcgc aagccgtcga
     9481 ggtcttcgtc gcccgcctcc agcgcggcgt cgccctgcgc gaccagcgcc tcccgcgccg
     9541 cgtccagcgc ctgcgcgatc ccgccatcgt ccggctgcag gcgttgcagc gccagcaaaa
     9601 gggggatcgc cgaatccgcg ctttcgaaca gatcgccggc ggccagtgcg cgcctggccc
     9661 gcgcgcgcgc ctgcacggca tcggacgcct gcaactggac ctgcggcagc gaccagctca
     9721 gcacctgcgc cagttcgtcg ccaccgctga tgcgcacgct gccggtgcgc tcgggcgcgg
     9781 ccggttgcgc ggcgggtacc ggcgcctgcg gcccgcagcc cgcaagcgcg atggtgacga
     9841 agaagcagag gggccagcct gcgcgcaaac cgcactccac gaatggcgaa tcgggcgcgg
     9901 ctaggttaag ccatcaccgc cgcgcaacgc ataatgcgcg gatgacgcat tggatcgaca
     9961 ccccggacgc gctgcgcgcg cacctcgccc agccgcccgc cagcgtcgga ctggataccg
    10021 aattcatccg cgagcgcacc tactggccgc agctcgcgct ggtgcaggtc gcgcttggcg
    10081 aggccgaccc ggcggcgccg gatgcgcaca tcctgctggt cgatccgctg gtgccgggca
    10141 tgtgcgaggc gctggtgccg ctgctggccg accccgcggt gctgaagatc atgcacagcg
    10201 ccagcgagga cctggtggcg ttccggcatg cctgcggggt gctgccagcg ccgttgttcg
    10261 atacccaggt cgcggcggcg ctggccgggg tcggcggcgg catgggctac cagaaactgg
    10321 tgcaggcgag caccggggtt acgctggcca agggcgagac ccgttccgac tggctgcgtc
    10381 ggccgctttc gccggcgcag ctcgactacg cggccgacga cgtgcgccac ctgcatgcgc
    10441 tgcatcgcca gctggacgcc gcgttgcagg cgcagggccg cagcggctgg ttgcaggaag
    10501 acgtggcgcg gctgctggcc accgtggccg acgaggacgg cgatcgctgg ccgcacctgt
    10561 cgcttcgcag cgcgcagttc ctcgacggcg acggccagcg ccgcctgctg cgcctgctgc
    10621 gctggcgcga ggcttacgcg cgccgcagcg accgcccgcg cagctggatc ctggacaacg
    10681 aactggcggt ggcgatcgca cgccagccgg cgctgggccg tggtggcctg cagcagttgc
    10741 tggacgccac cccgaaggca ccgcgcaacc tcggcgacgc gctgttcgag gcgttgtcct
    10801 cgccgctggc ggacgaggcc gacgccccgc ccgcgcgcgg cgacgaccgc gacaagaaac
    10861 agttgcgcgc gttgcaggac gcagtggcgg cggcatcggc ggaactcggg ctgcccgagg
    10921 gcctgctggc ttcacgacgc ctgctcgaga gcctgcagga cggcggcggc tggaccggcg
    10981 ccctggccgg ctggcggcgc gccgtgctgg aaccgcggct atcgcccctg ctcggtgctg
    11041 gcggcgcggc ggcggcatcc gtataatcgc cgtcgcagcc tgcatggggc ggtagctcag
    11101 ctgggagagc gccacgttcg catcgtggag gtcaggggtt cgatccccct ccgctccacc
    11161 aattccattg gtggatgcaa gatcaggaag ccggccccgt gccggctttt ttgttggccg
    11221 ccaccgcggt cgcgcgggcg ccacggcgac gtgcaagtgg ttatcgtagg ccgccgcgca
    11281 ggtcccggag tgacgccatg aaacgccccc tgatccttgt cgtggtcgcc ggactggccg
    11341 tgtgggccgg ctggacggtg ctgcagtcgc tgcgcgggcc gcagctcgcc ggctaccagg
    11401 tggcggcgcg gccgctggtg cagacggtgg tcgccagcgg ccgggtggtg gcggtgtcgc
    11461 gcgcgcaggt cggcagcccg gtgaccgggg tggtgctcga gcgccgcgtc cgcgagggcg
    11521 atgccgtgca gccgggcgac gtgctggcgg tgttgcgcgc ggacgacctg caggcggcgc
    11581 tgcgcgaggc cgaggccgcc gtcgcccagt tgcagcagtc cacccgcccg caggcgcagg
    11641 ccgcgctgcg cgaggccgag gcacggctgc agcaggccag ccgcgaagcc acccgcaggc
    11701 gcgacctgtt caagcgccag ctgatcgcgc gcgaggcgct ggagcaggcg ctgcaggccg
    11761 agacgatcgc ccgttccgcc gccgagcagg cgcggctgtc gctgaactcg ctttcagccg
    11821 gcaaccccaa cgaagccgcc gcgcgtgcac gcgcagccgc ggcacgcgcg cagctggcca
    11881 agaccatcat ccgcgccgaa gtggccggca ccgtgctgac ccgcaacgcc gagccgggcg
    11941 acctggtgca accgggccgg gtgctgttcg agatcgcccg cgccggcgcc accgaagtgc
    12001 tggttccgct ggacgagaag aacctggaag tgctggcgat cggccaggcc gcgcaatgcg
    12061 tggctgacgc ctatccgacg cggccgttcc cggcccaggt cagcttcatc gcgcccagcg
    12121 tggatccgca gcgcggttcg gtggacatcc gcctcacggt cagcccggtg cccgcattcc
    12181 tgcgccagga catgacggtg tcggtcaacg tcgaaaccgg ccgccgcgaa cgcgcggtgg
    12241 tcgtccccaa tgacgcgctg ggcccggtcg atggcaaccg tgcatcgctg tggcgggtcg
    12301 aggatggccg cgcggtccgc cgccaggtca cgctggggct gcgcggcctg accatggccg
    12361 aggtcaccga tggcctggcc gcgggcgact ggatcctggc cgacgccgcg gcgccgttgc
    12421 aggaaggcga ccgcgtgcgg gtcgtggccg gcgcgctgcc ggccgacgcc acggacgccg
    12481 ccacccgcaa ggaattgccg gccaagctgg actgatgccg atgtggatcg agtggaccat
    12541 cgccaccagg ttcctgcgcg agggcaagtc gcagagcacg ctgatcctgg tcggcatcgc
    12601 cgtcggggtg gcggtgatcg tgttcctgac cgcgctgatc accggcctgc agggcaacat
    12661 cgtcaaccgc acgctgggca cccaggcgca catcaaggtg cagccgaccg aggaagccaa
    12721 ccgcgtcctg gcgccggccg atggcagcgt gcacctggtg ctggaaagca agcgcgccca
    12781 gcgcctgcgc tcgatcaaca actggcaaca ggtgcgcgac gtgctggatg cgctgccacg
    12841 ggtcaccgcg gtgtcgccgg tgatctccgg gccggcgttc gcgcgccgtg gcgaagcgct
    12901 ggaatcggtg gcgctggtcg gcatcgacgc ggcgcgctac cagcgcatca tcccgatcgc
    12961 cgacgacatc gtcgccggcc gcttccggat cggcgccggc gaggcggtcg tcggcagcca
    13021 gctggcgcgc gacctcggcc tgcgggtcgg cgacaagctc cggctcgacg gcggccaggg
    13081 ccgcgacagc gtggtcaacg tggccgggat cttcgagctt ggcgtgcgcg aactcgacgc
    13141 gcgctacgtc tacctggaca tgaagcaggc gcaatcgctg ctcgacctgc cgggcgcggc
    13201 gaccatcatc gacgtcaccg tggacgacat cttcgccgcc agggaagtgg ccgcgcgcat
    13261 cgcccgcctc accgggctga aggccgagag ctggatggag accaacgccc agctgatgaa
    13321 cgcgctgcgc tcgcagagcc tgtcgacacg gatgatcagc gtgttcgtgg cattgagcgt
    13381 ggcgctgggg atcgccagcg tgctgtcggt gagcgtggtc cagcggaccc gcgagatcgg
    13441 catcctgcgc gcgatgggca ccacccggcc gcagatgctg cgggtgttcc tggtgcaggg
    13501 cgcgctgttc ggcctggccg ggtcgctggt cggcggagtg gccggctacg gcatggtgat
    13561 ggcgttcaac accttcggcc cgaagctgtt ctacatcccg gtggatccgt ggctgctggt
    13621 ggccgcctcg gtactggcca cggtcaccgg gatcgtctcc gcggcgatcc cggcgcgccg
    13681 cgccgccggc ctcgacccgg tggaggcgat ccgccatgtc tgaccgcgac gccggcccgg
    13741 acgtgctgcg cctgcagggc ctgcgcaaga gctacaacgt cggcaagccg accgaggtgg
    13801 aagtgctgca cggcctcgac ctgcgcctgc aacgccacga tttcgcggcg ctggtgggtg
    13861 cgtcgggctc cggcaagagc accctgctca acctggtcgg cctgctcgac acccccaccg
    13921 ccggcgaact gttcctgctc ggcgaagcca cccgcgacat ggacgatgcc cgccgcaccg
    13981 ccctgcgcgg tggtgcgatc ggcttcgtgt tccagttcca ccacttgatc caggccttca
    14041 ccgcgctgga aaacgtgctg atgccgctga tggtggccac cggcaagccc ggccgcgagc
    14101 acatcgaccg cgcccgcgcg ctgctggcgg aagtcggcct gcagggcctg gagcagcgca
    14161 agcccgacca gctctccggc ggccagcagc aacgggtggc agtggcgcgc gcgctggtga
    14221 cccgccccgc cctgctgctg gccgatgagc ccaccggcaa cctcgacagc cattcggcgg
    14281 cggaggtgtt cgaactgttc cgcaagttca accgcgaatt cggctgcgca gtgctgctgg
    14341 tcacccacga cccgcgcctg tccgaacagt gcgatcgcac ggtggcgctg gtggatggcc
    14401 gcatcgcaag cgacaccgcc aaggccggga aaacggagcc acggccaacc agcgcttgaa
    14461 tccgaaccgc gcacgattca acgccatccg cgacacgccc cggatgcaac ttccggtccg
    14521 caagcagacg ccggagcgcg gcctggacgc gcgcttgcct caggcgatcg ctgagccgcc
    14581 gccctgcgcc agcagtccgg ggaactgcgg cggttgcgag cgcaggtact gcggcggcac
    14641 cgcgaccgac gcgcccaggt gggcggcggc atgccagggc cagtgcgggt catagaggat
    14701 gccgcgggcg atggcgatga ggtcggcatc accgttggcg acgatcgcct cggcgtggtc
    14761 gaagccggtg atcatcccca ccgccaccac cggcatcgcc accgcctgct tcaccgcgcg
    14821 cgccagcggc acctggtagc cggggccggc cgcgatcgcc tgtgccgggt gcagcccgcc
    14881 gctggacacg tggatggcgc tgcagccgcg cgcttccagc gcctgcgcga acgcgatggc
    14941 ctgctcgatg tcccagccat ccggcatcca gtcggtggcg gacacgcgca cgctgaccgc
    15001 gcgcccggcc gggaatgcgt cgcgcacggc atcgaagacc tgcagcggga agcgcatgcg
    15061 gttttccagc gagccgccgt attcgtcgct gcgccggttc gacagcggcg acaggaactg
    15121 gtgcagcagg tagccgtgcg cggcgtggac ctgcaccgcg tcgatgccca gccgcgccgc
    15181 acggcgcgcg gcggcggcga acgcatcggt cacgccgtcg atgtccttgc gggtcatggc
    15241 gacgggggcg tggtcgtggt cgttgaatgg cagcgccgag ggcgcgagcg tctgccagcc
    15301 gcgggcctgc cccggcgcga cctggcggcc gccgtgccac ggcagcgcgc aggatgcctt
    15361 gcggccggca tgggccagct ggatcgcgac cggcatcgcc gaccagcggc gcacgccctg
    15421 cagcaccacc gccatcgccg cttcggtggc gtcgtcccac aggcccacat cgccatcggt
    15481 gatgcggcct tcgggcagga ccgcggttgc ttcgatggtg agcagcgccg cgcccgaatg
    15541 cgacagctgg cccagatgga tcaggtgcca gtcggtcatg cagccatcca ccgccgagta
    15601 ctggcacatc ggcgcgatca cgatgcggtt ggccagctgc agccggccca ccgccagcgg
    15661 ttcgaacagt cgtggcgtgc ccatgcgatc ccctcaaacg aagacggcgc ccgttgcggg
    15721 gcgccgtccg attgccaagc ctgccgcgcg gtcgacccgc cggcatgccg cctggatcag
    15781 gccttcttca cccaggcgct gcaccagccc ttggcggcaa ccgaattctt cgggaacaac
    15841 tggcacggcc cgtaggcctg ccccgcggtg cccttgtaga gcatgcagtt gctgcactcg
    15901 cggccgggct tgaaggtggc gtgcttcgag gtcgcggcct tttcgacata gccgagcgac
    15961 ttcgcctgcg cattgtccag cggcagcttc ggcagcgcct tggtctgcgc aacggcctgg
    16021 ttggcggcga gcggagccag cgccaccagc gcggcataac ccaggaaacg gcgacgaccg
    16081 tcgtcgtggt tttcactcat ggcggaactc ctctgtgtgg acgggaagcg gtgtgtgcgg
    16141 caggtgccgc gcgaaaccgg atgcggaacc gatgacccgc atggccgaag tttacgccca
    16201 cagcatgaat gcacgcacgc gttcgcgagc aactaggaat catttgcaat cagcaacttg
    16261 catcacgccg cgccactgcg caatcgccgg cacgccgccc tcgtgtggct acctcagggg
    16321 ccggtcgcgg cgtccggctt cgtcggctcc ggcgcatcca ccggtatgtc gtacagcagg
    16381 ccgggggtgg gttctagctg cagggtgaaa tgccaccatt ccatcgggta gttgcggaag
    16441 ccttccgcgg ccatcgccgc gcgcaggcgg tcgcggttgc ggcgctgcag cgcggtcact
    16501 tcggctgaat cggtattggc gcgggtgccg aagaaatcga agtccgtgcc catgtccacg
    16561 gcccggcaag cggcatcgtc ggcatcgcat tgcagcaggg tgaggtcgac cgtggccccg
    16621 cggctgtgcc cggaaaccgg tgcgatgtag ccgtccagca actgcggctt gtcgaagtcc
    16681 ggatagtgcc tggccttggt cgcgaggtcc gcggggtcgt tcgcccagcg catgaaatgc
    16741 gcgaccgcgc gcgtcgggcg gtagcagtcg aagatgcgca gccgctggtg gctttcgcgc
    16801 aggcggcgct cgacccgcgc cagcgcctcg gcggcctcgc gcttgagcca gcagtgcggc
    16861 gccaggtagc catcgacgcg ggtgccgacg aagttgtcgc tgccgaagta gcggatgtcc
    16921 tggtcgatgt ccggcaccac cgtgcggatg gcgaccatgc ccgcctcccg catggtcgcg
    16981 gccggggaca gcaccgggcc cgcgggcctg gccacgctgg cgcagccgat cgccgccagc
    17041 agcaacgtcg ccgccagcgc cagccgccta gtcacagcga ccgacccagg cgaacgcgag
    17101 gtgttcgtag tccgaactga aatcgccgtc cgggttgagc ttggccatcc gcagctggcg
    17161 cgcgtcgccg ctgccgctga agtcgagcca ggcgtgctcg tccaccgcga tgtcgtccca
    17221 gcgaagcacg tggcgcccat ccagcgtgcc gatcttgccg tgcatcttcg gcgacttcag
    17281 cgaacgccat tcgacgccgt cgccgaccgc gcacacgcgc acgtcgccga accacgggtc
    17341 gcgccagatg ccgagccagt cgcgcagcgc cgacgccgcc accggcttgc gagcgccgat
    17401 gtcgggcagt gcgggcgtgg cccccgctgc ggcggtggcg ggcttcgcca gttcgtcggc
    17461 ataacccgcg acccccaacg acctttccgg cgcggtgaac agcttgagca ggacctggcc
    17521 gagcacgccg cgcgcgcggt cgccatcgcc gttgatcatc agcacgaagc cggacctgcg
    17581 gtccgggacc agcatcatca tcgagtacat cccgccgagg gtgccggtgt gcgaaaccgt
    17641 ccacgcgccg tcggcgtcgg ccagccggaa gccgtaggca taggcatgga agtgggtgtt
    17701 gtcccagcgc ctgcgctgcg cggagatcgg catcggcgtg cg
//
LOCUS       DAORUK010000026        17324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_2971_length_17324_cov_18.120273, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000026 DAORUK010000000
VERSION     DAORUK010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 17324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17324
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2971_length_17324_cov_18.120273"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..555
                     /gene="cdd"
                     /locus_tag="PK818_03075"
     CDS             145..555
                     /gene="cdd"
                     /locus_tag="PK818_03075"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HQY81748.1"
                     /translation="MPVDVALLELAEQAGQNAYAPYSRLQVGAAVLTEAGGRYSGCNV
                     ENAAYPSGGCAEQHAIAAAVRAEGPGMRLAAVAISARDGRGESIAIPPCGACRQRIAE
                     FGEVVEIAFAGGDGDVVRSSLAVLLPQGFRLQAG"
     gene            complement(552..1970)
                     /locus_tag="PK818_03080"
     CDS             complement(552..1970)
                     /locus_tag="PK818_03080"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HQY81749.1"
                     /translation="MSTTDAIAAGGAPEPAHATAELPQELLRQLRDGLPLPAGALRGF
                     VQGIADGRVGDAQVGAFAMAVRLRGLPPADTAVLTLAMRDSGTCLDWGDAGLDGPLLD
                     KHSTGGIGDLASLTLGPMVAACGGYLPMLSGRGLGHTGGTLDKLEAIAGYRSQVDLPT
                     LRAVVREAGIAIVAAGGDLAPADRRLYAIRDVTATVDSIALITASILSKKLAAGTRSL
                     VLDVKCGSGATMDSREQSQALARSLVDVSCAAGLPCMALLTDMQQPLTDSVGNALELR
                     EALAYLRGEETPPRLHALCLALGSEMLCMGGLAGSDADARRQLLAARDSGRAFERFAK
                     MVHALGGRAGALEAGGGVAGAAPLVEPVFAAMPGRVAAIDARALGMAVVALGGGRSDP
                     GDAIDPRVGLTEVAEIGAGVAAGQPLALVHAASREALAAAQQRVRAAFRLVDPGMQVA
                     APELLLGRVTAGSEPAPADPAG"
     gene            2106..3299
                     /locus_tag="PK818_03085"
     CDS             2106..3299
                     /locus_tag="PK818_03085"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF006719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HQY81750.1"
                     /translation="MFETLQAAAADPILGLMARFREDQRAHKVDLGVGVYRDPSGRTP
                     VMRAVHAAERLRLEEEDSKSYQGMLGDVAFDAAIQSLCFGPSEAGIADRLATVHTPGG
                     TAALRTAADLVRAARPDARVWLADPSWDNHAPIFRAAGLEVRDYPYLDPARSGLRSAE
                     LLETLETVPAGDAVLFQASCHNPSGVDIDAATWERIAEIAGRRGFLPILDMAYQGLGD
                     GLDADAAGLRLLAAQLPEMLLTSSCSKNFGLYRERIGAVSVLAASAEHTRVAMSVLLR
                     GIRANYSMPPAHGAAVVARVLARPDLRADWEQELAGMRVHVAGMRDRLATTLAGLGCP
                     REALRRQRGMFSFVNLPADGARRLREEHAIYVLESGRINLAGLNDDSAVRFAHALQAV
                     GFTGD"
     gene            3436..5067
                     /gene="ipdC"
                     /locus_tag="PK818_03090"
     CDS             3436..5067
                     /gene="ipdC"
                     /locus_tag="PK818_03090"
                     /EC_number="4.1.1.43"
                     /EC_number="4.1.1.74"
                     /inference="COORDINATES: protein motif:HMM:TIGR03394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate/phenylpyruvate decarboxylase"
                     /protein_id="HQY81751.1"
                     /translation="MNLALQLLHALKAHGAREIFGIPGDFILPFFRHVEASGILPLVA
                     LSHEPSLGFAADAAARMNMGLGVVAVTYGAGALNTVNAIACAYAERSPVVVLAGCPGA
                     VEAHSGLALHHQVRHVDSQLRVFAEITCDQVRLDDPATAPALIARALRSCREFSQPVL
                     VEIPRDLAEREMGAVPMLPPSPFVQAAVDECAREWLARIQAAERPVLMPGVEVRRFGI
                     EAEVAELARRLALPVLTTFMGRGLLADAGLPCHATYLGVAGDPAISDLLDKSDLPVML
                     GLIQSDSNFGIAGERIDFRRALVASHREARIGHAVYHDIPLPALVRALLDQLPASDRT
                     RLADLPRPEYPTGLQDDDAALTAGDLARGLNDRIHAHGAFPVASDIGDCLFAAMELLP
                     TPLVAPGYYASMGFGVPAGIGMQVTTGERTVILVGDGAFQMTGWELGHCRRYGIDPIV
                     LLLNNQSWEMIRAFQPESKCADLGDWRYAEIADTLGGHGHRVRTRGEFKRALDAAFAE
                     RGRFQLIEMMVPAGDSTPTLKRFSEGIRALRARAA"
     gene            5300..6361
                     /gene="had"
                     /locus_tag="PK818_03095"
     CDS             5300..6361
                     /gene="had"
                     /locus_tag="PK818_03095"
                     /EC_number="1.1.1.368"
                     /inference="COORDINATES: protein motif:HMM:TIGR03201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxycyclohex-1-ene-1-carbonyl-CoA
                     dehydrogenase"
                     /protein_id="HQY81752.1"
                     /translation="MPAIDAWGMFLLEPRQPLQRRAFRIEPPAAGEVVVQVAGCGLCH
                     TDLGFIDGGVKTRHDLPLVLGHEISGTVIACGEGCEDWQGRQVVVPAVLPCGECELCA
                     AGRDNICQRQRMPGNDFHGGFASHLAVPARFLCALPGDLGGFPLAELSVVADAITTPY
                     QSVLRSGLAAGEVAIVIGVGGVGLYMVQHARNRGAQVIAIDVDPSRLQAAAAQGAAFT
                     VNAREFDERELRKHLRAACKARGWPDTRWRVFETSGTAAGQRSAYSLLSFAGTLGVVG
                     FTMDKTELRLSNIMAFDADVFGNWGCRPAHYPAVVADVLAGRIEVRRNVRQFPLSGIN
                     AVIADAQAHRLDARAVLVP"
     gene            6384..7463
                     /gene="oah"
                     /locus_tag="PK818_03100"
     CDS             6384..7463
                     /gene="oah"
                     /locus_tag="PK818_03100"
                     /EC_number="3.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase"
                     /protein_id="HQY81753.1"
                     /translation="MKNHHLIDRCDYRDILVEKRPLRDAHGAEVPGLYNAWILLNNEK
                     QLNSYTTQAVKELILAFREASCDRSVVAVVFSAVGDKAFCTGGNTKEYAEYYAGNPQE
                     YKQYMRLFNDMVTGILLCDKPVINRVNGMRIGGGQEIGMACDFSIAADTARFGQAGPK
                     HGSAPDGGSTDFLHLFVGITRAMASCTLCEHWSAHAAVDIGLVNKIVPVLKAPDGRFI
                     GNPMVSLERFDAWGNPAYGAFKSGTEREAAKAAMAGCTTDLSLLDAEVEAMAYKLSLT
                     MPDCLAKTLESVRKKKLEHWQRNGESNRSWLALNMMTEARAGFRAFNEGPRHQREVDF
                     LELRRALAEAHPWDDALLARIMPKA"
     gene            7468..8238
                     /locus_tag="PK818_03105"
     CDS             7468..8238
                     /locus_tag="PK818_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQY81754.1"
                     /translation="MDFAKLALRETHDGQVLEVALDAPKANILDAAMMAELRAVLAAE
                     RGVAGRKALVLRGSGAHFCFGASVPEHQADQVGAMLPQFHALIDDLLSHPVPTIAQVS
                     GFCLGGGFELALACALLFADDSARFAVPEIQLGVFPPVAAVLLPALGAAMLGNRLVLS
                     GETVDADALAGCGLLAGRGDSAEAAASAWIGQHLLPRSASSLRIAQRAARAAVLAQFR
                     AHIGAAERLYLDQLMATHDANEGIAAFIGKRPPAWTDA"
     gene            8240..8899
                     /locus_tag="PK818_03110"
     CDS             8240..8899
                     /locus_tag="PK818_03110"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY81755.1"
                     /translation="MDAQAIPASSQPADDILAECAALMAREGYHGTSMRDLAAATGRS
                     LAGLYHHFSSKEELLFLINERGFASLLANAERLLAAYRGGRGSAQRPSRKASAALLCD
                     LIGAHLGYFGSHLNEMRILMFGTQPMSSAHGEAIRALKQRYTDCVQLAVGDYIAARRE
                     PLAAGDVERRTYLLFGMMNWIFGWYSFDQHGSVDALAEEIFLTFTRGCLPRAQATHGE
                     G"
     gene            8905..10065
                     /gene="bcrC"
                     /locus_tag="PK818_03115"
     CDS             8905..10065
                     /gene="bcrC"
                     /locus_tag="PK818_03115"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit C"
                     /protein_id="HQY81756.1"
                     /translation="MGIKAIIDRCERLYRDVDLGCVQEWKARTGGKAVGHMPVYVPRE
                     LIHAAGLLPVGVMGGGDDVEIIRGDAYFQSYICHIPRSTVELGLAGKLDCLDGMLFPA
                     ICDVIRNLSGMWQLMFPGKYVKYIDFPQTFNGAGREFYRFELASLLLDMEQLSGVRAT
                     RERLNQSIALYNDNRRALAALYDLRAAEPSKVPTSELYLLLRAGNVLEVSEHTAILRE
                     YLQLARERGRPRHDNVRVVVTGLFCEQPPLSMVRAMENSGCYIVDDDWVLGARYQLGD
                     TAIGERPLDAIVDAYMDGTVATATRYVEGDDKGAFLVEQVRQRRAEGVIFAAASFCDP
                     ALLDRPMGQKALADAGIPYTSFTYSENTGQVQVIKEQAGTFADSIKLWGVAA"
     gene            10062..11333
                     /gene="bcrB"
                     /locus_tag="PK818_03120"
     CDS             10062..11333
                     /gene="bcrB"
                     /locus_tag="PK818_03120"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit B"
                     /protein_id="HQY81757.1"
                     /translation="MNASVQKEESMVMQKKMIGEHFETLARAPESGRKVVYTFVPGNL
                     NELILAFDLLPVHPEVNALQSGMRKLSGDYIQEAEKQGHSEDVCTYVKCDIGMLKKGN
                     IGPTGEKLPPPDLLLLSYTGCFTFMKWFELLKQEYGCPVVMLQVPYQADGTITASQRE
                     FVARQLREVVVPALEEVSGKKLDEDRLKELLASSARAEDDLVYVWESAKHRPSPIDAY
                     FGGVYYIGPIFTAFRGLPDCEAYYRSLRSEVDYRIAHGMGPTTPEGELREQKYRIVVE
                     GPPNWTSFRDFWKIFHDEGVVMVASTYTKVGGLYEQGFRHDPAKPFESLAEYCLGCYT
                     NRNLPARVDIIAKYVEEYEADGILFNSIKSCNSFSAGQLMMLRELEKRTGKSGAFIES
                     DLVDPRYFSAANIKNRLESYFQMLEAKRQAA"
     gene            11344..12732
                     /locus_tag="PK818_03125"
     CDS             11344..12732
                     /locus_tag="PK818_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HQY81758.1"
                     /translation="MDCCYIGIDLGSTTTKAVFLDEQERIMGRGITNSRSNYELACQV
                     AREEAEINARFGLLRRTLEASERIGELAEPLMAVLACNHALAQHLEQLQALDAECRAC
                     AQAHDLPATEQAGIEQALARIFAALRTQAELDFFPGSAAASDFFRDLATSHYLQQAEQ
                     VTRELPVSFDRLVGLFDAAIIRVENHSFAGLYREYLAFATRRALADARFAPFADEIAA
                     AVGETAERRFNVLNTVGTGYGRLTLPFAREQIRSEILCHGLGAHYMFPDTRTVLDIGG
                     QDTKAIQVDGNGIVTGFQMNDRCAAGCGRYLGYIADEMSIGVHELGPLALQSTKPVKI
                     NSTCTVFAGAELRELMALGEKREDILAGLHRAIVLRAMSLLARSGGVESEFTFTGGVA
                     RNQAVTGALEHLVFENYGKMTINISADSIFTGAVGAALFAKRDPKGVMSDLEVSKLPG
                     SKACGLAAACPA"
     gene            12745..13596
                     /gene="bcrD"
                     /locus_tag="PK818_03130"
     CDS             12745..13596
                     /gene="bcrD"
                     /locus_tag="PK818_03130"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit D"
                     /protein_id="HQY81759.1"
                     /translation="MFHTVGIDVGCDSVKTSLVAFDDDGGNPRLLFKDLDKIRKRNVK
                     DVVRSAFERALLREGLSEAGIAYIATTGEGELVDFRTGHFFSMTAHARGAIFLDPGVR
                     AVVDAGALHAKAMVVDERSKVIAYRMTSQCASGSGQFLENIARYLGVAIDDIGALSLQ
                     GDSPEQVSGICAVLAETDVINMVSRGISTANILRGIHLSMAKRLIGLMRMIKTSGDVL
                     VTGGLARNAGFVAAMQELAAQDKRLKANIVVHPDSVHAGAIGAALWAGYRHLRLEAPA
                     LAQPEAA"
     gene            13596..15353
                     /locus_tag="PK818_03135"
     CDS             13596..15353
                     /locus_tag="PK818_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQY81760.1"
                     /translation="MSEHAAHAMPILRNPQALAAVAPNIGSLVLDNAARHDGAPAFAE
                     RVDGRYASHSWRQFGADLLRVAAWLGQRERVAFVAGNSYLRHVAELATMAAGGASVPV
                     FAGYPAALMGELLRFSDVELLVTDQPDKVLALDPACLPRHLLVLAEPRPYDPSAYLAG
                     GGRIDRIGAVLAREPDAASLARVERELRAVAAGQTCMIMYTSGTSGFPKGVQLTHHNL
                     LSQQQALAQCWQPQPGLRMLCYLPWHHSFGGLFERFFAVAFGGCLALDDGGGRDVDRL
                     LENFAEIRPHLFFSVPKIYQEIAARVQASAGVERAFFHPELRFVFTAAAPLPLSASDV
                     FRAHAVPVVEGWGLTETSPCCTLTPFSLDRTPGVVGLPIPGVEVALAADGEILVRGAN
                     VMQGYFRNPEATAAAFTDDGWFRTGDIGEFGAEGVRIRSRKERMFKLSNGEKVFPAQI
                     EARIHARCQFVKYAYVFGSGQRTPHLLVFPNQQAFEGCGSSNGCAHPNDAGQLAHCLS
                     NCMAEINARPAAGFETIRRALVVGRELSIDENELTPSFKLIPKRIEERYADCIEAMEQ
                     GRYEHLPADVFVLDLEERK"
     gene            15350..16273
                     /locus_tag="PK818_03140"
     CDS             15350..16273
                     /locus_tag="PK818_03140"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HQY81761.1"
                     /translation="MSIHYGVIGTGPVGRVFAGLLRQAGHRVSVLAHRAQTQQILQRR
                     PVVVKGKLEAYAQLQDVVLETGDFVRAAPDVVLLCTKSVDSEHVLRTLREHAPKPGML
                     FVSCQNGIGTERVIEAVFGRERALRIVLNMGCDVSCGNEVIVTFCTTHVMGRSPHVDP
                     ARIAAIAADLKDSGLPLELRDDWREDVFRKALLNASLGSMCAVTRHCMGCVMGQPEAR
                     AMVEDMLREGMAICSAEGIELGPDFLGFAMHYLDQGGEHRPSILVDVEKGKVTENEFH
                     CGELARLARKHGVAAPTIHFVDKVLRCAENV"
     gene            16273..>17324
                     /locus_tag="PK818_03145"
     CDS             16273..>17324
                     /locus_tag="PK818_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HQY81762.1"
                     /translation="MRGFRNVAVVGAGTMGAAIAQHFLMKGLQVHLADANQAGLARGQ
                     GLVAASLDEAVARRLLDADGRAATLARLHPTTDLADLAGVDLVVEAVFEDLDVKRALF
                     AQLEGIVRTDCVLATNTSSFRVGDVAAGLAHPGRVLGTHYFYHAAKNKLVELIACEHT
                     APDLLAAVEAFYAGLGKTPIRTADAPGFAVNRFFVPWLNEAVRLHAEGLGSIPGIDRV
                     AREVFGVGMGPFALMNATGVPIAQHAAEGLAATLGDFYAPAPRLVEQVAARADWNLDD
                     CTVPHGGAEQPQVVRERLLAAALGCAAALASEGVCSATDTDLGARAGLRWPRGPFELM
                     AAIGQDAIAGMVGALFA"
BASE COUNT         2583 a         6234 c         6025 g         2482 t
ORIGIN      
        1 agccaacccg caggcacgaa ggccccgcaa cgcggggcct tcgcgttgca cccggacgcg
       61 gcaccacgtg gcccgggatc gaccgccgct ttaccgcgac ccgccggcgg ccgatgatgg
      121 cggccccgtc catggatcga tcgcatgccc gttgatgttg ccctgctgga attggccgaa
      181 caggccgggc agaacgccta cgcaccgtat tcgcggctgc aggtcggggc cgcggtcctg
      241 accgaggcgg gcggccgtta cagcggctgc aacgtcgaga acgccgccta tcccagcggc
      301 ggctgcgcag agcagcatgc gatcgccgcg gcggtgcgcg cggaaggccc gggcatgcgc
      361 ctggcggcgg tggcgatcag cgcccgcgat ggccgcggcg agtcgatcgc gatcccgccg
      421 tgcggcgcct gccggcagcg catcgccgag ttcggcgagg tggtcgagat cgcgttcgcc
      481 ggcggcgacg gcgatgtcgt gcgcagcagc cttgccgtgt tgttgccgca gggcttccgc
      541 ctgcaggcag gttagccggc cggatccgcc ggcgccggct cgctgccggc ggtcacgcgc
      601 ccgagcagca gttcgggtgc ggcaacctgc atccccggat cgaccagccg gaaggccgcg
      661 cgcacgcgtt gctgtgcggc cgccagcgcc tcccggctgg cggcgtggac cagcgccagc
      721 ggctgcccgg cggcgacccc tgcgccgatt tcggcgacct cggtcagtcc cacgcgcggg
      781 tcgatggcat cgcccggatc gctgcggccg ccgcccagcg ccaccaccgc catgcccagc
      841 gcacgcgcgt cgatcgcggc cacccggccc ggcatcgccg cgaacaccgg ttccaccagc
      901 ggtgccgcgc cagccacgcc gccaccggct tccagcgcac cggcgcgccc gcccagggca
      961 tgcaccatct tcgcgaatcg ttcgaacgcg cggccgctgt cgcgcgcggc cagcaattgc
     1021 cggcgtgcgt cggcgtcgct gccggcgagg ccgcccatgc acagcatttc gctgcccagc
     1081 gccaggcaca gcgcatgcag gcgcggcggg gtttcctcgc cacgcaggta ggcgagcgcc
     1141 tcccgcagct ccagcgcgtt gccgaccgaa tcggtcagcg gctgctgcat gtcggtcagc
     1201 agcgccatgc acggcaatcc ggccgcgcag gaaacatcca ccagcgaacg cgccagcgcc
     1261 tggctctgtt cccggctgtc catggtggcg ccgctgccgc acttcacgtc cagcaccagc
     1321 gatcgggtgc cggccgccag cttcttggac aggatcgagg cggtgatcag cgcgatcgaa
     1381 tcgaccgtgg cggtgacgtc gcggatcgcg tacaggcggc gatcggccgg ggccaggtcg
     1441 ccgccggcgg cgacgatggc gatcccggcc tcgcgcacca ccgcgcgcag ggtcggcagg
     1501 tccacctgcg agcggtaacc ggcgatcgcc tcgagcttgt cgagggtgcc gccggtgtgg
     1561 ccgaggccgc gcccggagag catcggcagg taaccaccgc aggccgccac catcgggccc
     1621 agggtgaggc tggcgaggtc gccgatgccg ccggtcgaat gcttgtccag caacgggcca
     1681 tcgagtccgg catcgcccca gtccaggcag gtgccactgt cgcgcatggc cagggtgagc
     1741 accgcggtgt cggccggcgg caacccgcgc aggcgcacgg ccatcgcgaa cgccccgacc
     1801 tgggcgtcgc cgacccggcc atcggcgatc ccctgcacga agccgcgcag cgcgccggcc
     1861 ggcaacggca ggccgtcgcg tagctgccgc agcagctcct gcggcagttc tgcggtggcg
     1921 tgggcgggtt cgggtgcgcc gccggcggcg attgcatcgg tggtcgacat cgttcccgga
     1981 gtgtgacgcc ggaccgccga ggttgccagc atcgcggcct gccagtggcg gcgcccgtcc
     2041 acgcgcatgg cggcaggcgc ccgcatcgtg gacacttgca ctccccctcc gcacggcgca
     2101 ccccgatgtt cgagaccctg caagcggccg cggccgaccc gatcctgggc ctgatggcgc
     2161 ggttccggga agaccagcgc gcgcacaagg tcgaccttgg cgtgggcgtg taccgcgatc
     2221 cctccggccg caccccggtg atgcgcgcgg tgcacgccgc cgaacgcctg cgcctggagg
     2281 aggaagacag caagagctac cagggcatgc tcggtgacgt ggcattcgac gccgcgatcc
     2341 agtcgctgtg cttcggcccg tcggaggccg gcatcgccga ccgcctggcc accgtgcaca
     2401 cgcccggcgg caccgccgcg ctgcgcaccg ccgccgacct ggtgcgcgcc gcgcgcccgg
     2461 atgcgcgggt gtggctggcc gatccgagct gggacaacca tgcgccgatc ttccgcgccg
     2521 caggcctgga ggtgcgcgac tacccctacc tggacccggc gcgcagcggc ctgcgcagcg
     2581 cggaactgct ggagacgctg gagaccgtcc cggccggcga tgcggtgctg ttccaggcca
     2641 gttgccacaa ccccagcggg gtcgacatcg acgccgccac ctgggagcgg atcgccgaga
     2701 tcgccggccg gcgcggcttc ctgccgatcc tcgacatggc ctaccagggc ctgggcgacg
     2761 gcctggacgc ggacgccgcc ggcctgcgcc tgctggccgc gcaacttccg gaaatgttgc
     2821 tgacctcctc ctgctccaag aacttcgggc tgtaccgcga gcgcatcggt gcggtcagcg
     2881 tgctggccgc gtccgccgaa cacacccggg tggcgatgtc ggtgctgttg cgcgggatcc
     2941 gcgccaacta ctcgatgccg ccggcgcacg gcgcggccgt ggtggcgcgg gtgcttgcgc
     3001 ggccggacct gcgcgcggac tgggagcagg aactggcggg gatgcgggtc cacgtcgccg
     3061 gcatgcgcga ccgccttgcg accaccctgg ccggtctcgg ctgcccgcgc gaggcgctgc
     3121 ggcggcaacg cgggatgttc tcgttcgtca acctgccggc cgacggtgcc cgccggctgc
     3181 gcgaagagca cgccatctac gtgctggaat cgggccggat caacctcgcc gggctgaacg
     3241 acgacagcgc cgtgcgcttc gcccacgcat tgcaggcggt gggcttcacc ggcgattgag
     3301 cgcaacgccc gtaccttcgc cgcagcgttg gcgcaggcat acgcaagcgc acggcgcggg
     3361 cgttctgacg caggtcaatg acagccgcgc gcggttgctg tctgatgccg cgctaccctc
     3421 cgtccccgag ccgccatgaa ccttgccctg caattgctgc acgccctcaa ggcgcacggc
     3481 gcccgcgaga tcttcggcat cccgggtgat ttcatcctgc cgttcttcag gcacgtggag
     3541 gccagcggga tcctgccgct ggtcgcgctc agccacgaac cgtcgctggg cttcgccgcg
     3601 gatgccgccg cgcgcatgaa catggggctg ggcgtggtcg cggtgaccta cggcgcgggc
     3661 gcgctcaata ccgtcaacgc gatcgcctgc gcctacgccg agcgttcgcc ggtggtggtg
     3721 ctggccggtt gcccgggcgc ggtcgaggcg cactccgggc tggccctgca ccaccaggtg
     3781 cggcacgtgg attcgcaact gcgggtgttc gccgagatca cctgcgacca ggtgcggctg
     3841 gacgacccgg ccaccgcgcc ggcgctgatc gcgcgcgcgc tgcgcagttg ccgcgagttc
     3901 tcgcagccgg tgctggtcga gatcccgcgc gacctcgccg agcgcgagat gggcgcggtg
     3961 ccgatgctgc cgccaagccc gttcgtgcag gcggcggtgg acgagtgcgc gcgcgaatgg
     4021 ctggcgcgca tccaggccgc ggaacggccg gtgctgatgc cgggagtgga ggtgcgccgt
     4081 ttcgggatcg aggccgaggt ggccgaactg gcgcggcggc tggcgctgcc ggtgctgacc
     4141 acgttcatgg gccgcggcct gctggcggat gccggcctgc cttgccatgc cacctacctc
     4201 ggggtcgccg gcgaccccgc gatcagcgac ctgctggaca agtccgacct gccggtgatg
     4261 ctgggcctga tccagtccga cagcaatttc ggcatcgccg gcgagcgcat cgacttccgc
     4321 cgcgcgctgg tggcctcgca ccgcgaggcg cggatcggcc acgccgtcta ccacgacatc
     4381 ccgctgccgg cgctggtgcg cgccctgctc gaccagctgc cggccagcga ccgcacgcgg
     4441 ctggcggact tgccgcgtcc cgaatacccc accggcctgc aggacgacga cgccgcgctg
     4501 accgccggcg acctcgcgcg cgggctgaac gaccgcatcc acgcgcacgg cgcgtttccc
     4561 gtggcctccg acatcggcga ctgcctgttc gcggcgatgg agctgctgcc cacgccgctg
     4621 gtcgcgcccg gctattacgc ctcgatgggc ttcggggtgc cggccgggat cggcatgcag
     4681 gtcaccaccg gcgagcgcac cgtgatcctg gtcggcgacg gcgcgttcca gatgaccggc
     4741 tgggaactgg gccactgccg ccgttacggc atcgacccga tcgtgctgct gctcaacaac
     4801 cagagctggg aaatgatccg cgcgttccag ccggaatcga agtgcgccga ccttggcgac
     4861 tggcgctatg ccgagatcgc cgacaccctt ggcggccacg gccaccgcgt gcgcacccgc
     4921 ggcgagttca agcgcgcgct ggatgccgcg ttcgccgagc gcggccgctt ccagctgatc
     4981 gagatgatgg tgcccgcggg cgacagcacg ccgaccctga agcgcttctc cgagggcatc
     5041 cgcgccctgc gcgcgcgcgc cgcctgaccc cgcggcgggg ccacccgcac ccgcccaggg
     5101 ccgtggccgc gagcccgttc caaccagctg gccgcggccc ccgacgccgc gtttccggcc
     5161 gcgcgcgaac accccaccaa ccaccgcgtc cgcctgcccg cgccggcggt tgaccgcgcc
     5221 cgcccgaccc cgtagatttc acccaagagc cgaacgaacg ttcggctggt caccgaacac
     5281 cgacctgggg aggttgcgca tgccggccat cgacgcctgg gggatgttcc tgctggaacc
     5341 ccggcagccg ctgcaacggc gcgcgttccg gatcgagccg ccggctgcgg gcgaagtggt
     5401 ggtgcaggtc gccggctgcg ggctgtgcca caccgacctt ggcttcatcg acggcggggt
     5461 caagacccgc cacgacctgc cgctggtgct ggggcacgag atctccggca ccgtgatcgc
     5521 ctgcggcgag ggttgcgaag actggcaggg ccgccaggtg gtggtgccgg cggtgctgcc
     5581 ctgcggcgaa tgcgaactgt gcgcggccgg ccgcgacaac atctgccagc gccagcgcat
     5641 gcccggcaac gacttccacg gtggcttcgc cagccacctg gcggtaccgg cgcgcttcct
     5701 gtgcgcactg ccaggcgacc tcggtggctt cccgctggcc gaactcagcg tggtcgccga
     5761 tgcgatcacc acgccctacc agtcggtact gcgcagcggc ctggccgccg gcgaagtggc
     5821 gatcgtgatc ggcgttggcg gggtcggcct gtacatggtc cagcacgcac gcaaccgagg
     5881 cgcgcaggtg atcgcgatcg acgtcgatcc atcccgtttg caagcggccg cggcgcaggg
     5941 ggcggcattc accgtcaatg cccgcgaatt cgacgagcgc gagctgcgca agcacctgcg
     6001 cgcggcctgc aaggcccgcg gctggccgga tacccgctgg cgcgtgttcg agacctccgg
     6061 caccgccgcc ggccagcgca gcgcgtattc gctgctgagc ttcgccggca ccctgggcgt
     6121 ggtcggtttc accatggaca agaccgagct gcgcctgtcc aacatcatgg ccttcgatgc
     6181 cgacgtcttc ggcaactggg gctgccggcc cgcgcactac ccagcggtgg tcgccgacgt
     6241 gctggccggg cgcatcgagg tgcgccgcaa cgtccgccag ttcccgcttt ccgggatcaa
     6301 cgcggtgatc gccgacgccc aagcgcaccg cctcgacgcg cgcgccgtgc tggtgccctg
     6361 acccccttgc cttcgagccg acgatgaaga accaccacct gatcgaccgc tgcgactacc
     6421 gcgacatcct ggtcgagaag cgcccgctgc gcgatgcgca cggcgccgaa gtgcccggcc
     6481 tgtacaacgc ctggatcctg ctcaataacg agaagcagct gaactcctac accacccagg
     6541 cggtgaagga gctgatcctg gccttccgcg aggcctcctg cgaccgcagc gtggtcgcgg
     6601 tggtgttcag cgcagtcggc gacaaggcct tctgcaccgg cggcaacacc aaggagtacg
     6661 ccgagtacta cgccggcaac ccgcaggaat acaagcagta catgcggctg ttcaacgaca
     6721 tggtcaccgg catcctgctg tgcgacaagc cggtgatcaa ccgcgtcaac ggcatgcgca
     6781 tcggcggtgg ccaggagatc ggcatggcct gcgatttcag catcgccgcc gacaccgcgc
     6841 gcttcggcca ggccggtccc aagcacggct cggcgccgga cggcggctcc accgatttcc
     6901 tgcacctgtt cgtcggcatc acccgcgcga tggcgtcctg caccctgtgc gaacactggt
     6961 cggcgcatgc cgcggtcgac atcggcctgg tcaacaagat cgtgccggtg ctgaaggcgc
     7021 ccgacggccg cttcatcggc aacccgatgg tctcgctgga acgcttcgac gcctggggca
     7081 acccggccta cggtgcgttc aagtccggca ccgaacgcga ggcggcgaag gcggcgatgg
     7141 ccggatgcac gaccgacctc tcgctgctcg atgccgaggt cgaggcgatg gcgtacaagc
     7201 tttcgctgac catgcccgac tgcctggcca agaccctcga gtcggtgcgc aagaagaagc
     7261 tcgagcactg gcagcgcaac ggcgagagca accgctcgtg gctggcgctg aacatgatga
     7321 ccgaggcccg cgccggcttc cgcgccttca acgaaggccc caggcaccag cgcgaggtcg
     7381 acttcctgga actgcgccgc gcgctcgccg aggcgcatcc gtgggacgac gcgctgctgg
     7441 cgcggatcat gccgaaggcc tgacgccatg gacttcgcca agctcgcact gcgcgaaacc
     7501 cacgacggcc aggtgctgga ggtggcgctg gacgcgccca aggccaacat cctcgatgcc
     7561 gcgatgatgg ccgagctgcg cgcggtgctg gccgccgagc gtggcgtggc cgggcgcaag
     7621 gcgctggtgc tgcgcgggtc cggcgcgcat ttctgcttcg gcgccagcgt gcccgagcac
     7681 caggccgacc aggtcggggc gatgctgccg cagttccacg cgctgatcga cgacctgctt
     7741 tcgcatccgg tgccgaccat cgcccaggtt tcgggcttct gcctcggcgg cggcttcgaa
     7801 ctggcgctgg cctgcgcgct gcttttcgcc gacgacagcg cgcgcttcgc ggtgccggag
     7861 atccagctcg gggtgttccc gccggtggcg gcggtgctgc tgccggcgct gggcgcggcg
     7921 atgctcggca accgcctggt gctgtcgggc gaaaccgtcg atgccgatgc gctggccggc
     7981 tgcggcctgc tcgccggtcg cggcgacagt gccgaggccg cggcatcggc gtggatcggg
     8041 cagcacctgt tgccgcgcag cgccagcagc ctgcgcatcg cccagcgtgc cgcgcgcgcc
     8101 gcagtgctgg cgcagttccg cgcccacatc ggcgccgccg agcgcctgta cctcgatcaa
     8161 ctgatggcca cccacgatgc caacgagggc atcgccgcct tcatcggcaa gcgcccgccg
     8221 gcgtggacgg acgcctgaga tggacgccca ggccatcccc gccagcagcc agccggcaga
     8281 cgacatcctc gccgagtgcg cggcgctgat ggcgcgcgag ggttaccacg gcacctcgat
     8341 gcgcgacctc gccgccgcca ccgggcgctc gctggccggg ctctaccacc acttctccag
     8401 caaggaggag ctgctgttcc tgatcaacga gcgcggcttc gcctcgctgc tggcgaacgc
     8461 ggagcgcctg ctggccgcct accgcggcgg ccgcggcagc gcgcaacggc cgtcgcgcaa
     8521 ggccagcgcg gcgctgctgt gcgacctgat cggcgcccac ctgggctatt tcggcagcca
     8581 cctcaacgag atgcggatcc tgatgttcgg cacccagccg atgagcagcg cgcacggcga
     8641 ggcgatccgc gcgctgaagc agcgctacac cgactgcgtg cagctggcgg tcggcgacta
     8701 catcgcggcg cgccgcgagc cgctcgcggc cggcgacgtc gaacgcagga cctacctgct
     8761 gttcggaatg atgaactgga tcttcggctg gtattcgttc gaccagcacg gctcggtgga
     8821 cgcgctggcc gaggagatct tcctgacatt cacccgaggc tgcctgccgc gcgcgcaggc
     8881 gactcacggc gagggctagc gcacatgggc atcaaggcga tcatcgaccg ctgcgaacgg
     8941 ctgtaccggg acgtcgacct cggttgcgtg caggagtgga aggcgcgcac cggcggcaag
     9001 gcggtcgggc acatgccggt gtacgtgccg cgcgagctca tccacgccgc cgggctgctg
     9061 ccggtcgggg tgatgggcgg cggcgacgac gtcgagatca tccgcggcga cgcctacttc
     9121 cagtcctaca tctgccatat cccgcgctcg acggtggaac tgggcctggc cggcaagctg
     9181 gattgcctgg acggcatgct gttcccggcg atctgcgacg tgatccgcaa cctctcgggg
     9241 atgtggcagc tgatgttccc cggcaagtac gtcaagtaca tcgacttccc gcagaccttc
     9301 aacggcgccg gccgcgagtt ctaccgcttc gagctggcca gcctgctgct cgacatggag
     9361 cagctgtccg gcgtccgcgc cacccgcgag cgcctgaacc agtcgatcgc gctgtacaac
     9421 gacaatcgcc gcgcgctggc cgcgctgtac gacctgcgcg ccgccgagcc cagcaaggtc
     9481 ccgaccagcg agctgtacct gctgctgcgc gccggcaacg tgctggaggt atccgagcac
     9541 accgcgatcc tgcgcgaata cctgcagctg gcgcgcgaac gcgggcggcc gcgccacgac
     9601 aacgtgcggg tggtggtgac cgggctgttc tgcgagcagc cgccgctgtc gatggtgcgc
     9661 gcgatggaga acagcggctg ctacatcgtc gacgacgact gggtgctggg cgcgcgctac
     9721 cagctgggcg acaccgcgat cggcgagcgg ccgctggacg cgatcgtgga tgcctacatg
     9781 gacggcaccg tggccaccgc cacccgctac gtcgaaggcg acgacaaggg cgcgttcctg
     9841 gtcgagcagg tgcgccagcg tcgcgccgag ggcgtgatct tcgccgcggc gagcttctgc
     9901 gacccggccc tgctggaccg cccgatgggg cagaaggcgc tggccgacgc cggcatcccc
     9961 tacacctcct tcacctactc cgagaacacc ggccaggtcc aggtgatcaa ggaacaggcc
    10021 ggcaccttcg ccgattcgat caagctctgg ggagtcgcgg catgaacgcc agcgtacaga
    10081 aagaagaaag catggtgatg cagaagaaga tgatcggcga gcacttcgag acgctcgcca
    10141 gggcgccgga gagcgggcgc aaggtcgtct acaccttcgt gcccggcaac ctcaacgagc
    10201 tgatcctggc attcgacctg ctgccggtgc acccggaggt caacgccctg cagtcgggca
    10261 tgcgcaagct gtccggcgac tacatccagg aagccgagaa gcagggccat tccgaggatg
    10321 tctgcaccta cgtcaagtgc gacatcggca tgctcaagaa gggcaacatc ggccccaccg
    10381 gcgagaagct gccgccgccg gacctgctgc tgctcagcta caccggctgc ttcaccttca
    10441 tgaagtggtt cgagctgctc aagcaggaat acggctgccc ggtggtgatg ctgcaggtgc
    10501 cctaccaggc cgacggcacc atcaccgcca gccagcgcga gttcgtcgcc cgccagctgc
    10561 gcgaagtggt ggtgccggcg ctggaggaag tgtccggcaa gaagctcgac gaggaccggc
    10621 tcaaggaact gctggcgagc tcggcgcgcg ccgaggacga cctcgtgtac gtgtgggagt
    10681 cggccaagca ccggccgtcg ccgatcgacg cctacttcgg cggcgtgtac tacatcggcc
    10741 cgatcttcac cgccttccgc ggcctgcccg actgcgaggc ctactaccgc tcgctgcgca
    10801 gcgaggtgga ctaccgcatc gcccacggca tgggcccgac caccccggaa ggcgagctgc
    10861 gcgagcagaa ataccggatc gtggtcgagg gcccgccgaa ctggaccagc ttccgcgact
    10921 tctggaagat cttccacgac gagggcgtgg tgatggtggc ctcgacctac accaaggtcg
    10981 gcggcctcta cgagcagggc ttccgccacg acccggcgaa gccgttcgag agcctggccg
    11041 agtactgcct gggctgctac accaaccgca acctgccggc gcgggtggac atcatcgcca
    11101 agtacgtcga ggagtacgag gcggacggca tcctgttcaa ctcgatcaag agctgcaact
    11161 cgttctccgc cgggcagctg atgatgctgc gcgagctgga gaagcgcacc ggcaagtccg
    11221 gcgcgttcat cgaatcggac ctggtcgacc cgcgctattt cagcgcggcc aacatcaaga
    11281 accgcctcga atcctacttc cagatgctgg aagccaagcg ccaggccgcc tgaggagaca
    11341 cccatggact gctgctacat cggcatcgac ctcggctcga ccaccaccaa ggcggtgttc
    11401 ctggacgagc aggaacgcat catgggccgc ggcatcacca actcgcgcag caactacgag
    11461 ctggcctgcc aggtcgcgcg cgaggaggcc gagatcaacg cccgcttcgg cctgctgcgg
    11521 cgcaccctgg aggccagcga gcgcatcggc gaactggcgg agccgctgat ggcggtgctg
    11581 gcctgcaacc acgcgctggc gcagcacctg gagcagctgc aggcgctgga tgccgaatgc
    11641 cgggcatgcg cgcaggcgca cgacctgccg gccaccgagc aggccggcat cgagcaggcg
    11701 ctggcgcgca tcttcgcggc gctgcgcacg caggccgagc tcgacttctt ccccggcagc
    11761 gccgccgcca gcgacttctt ccgcgacctc gccaccagcc actacctgca gcaggccgaa
    11821 caggtcaccc gcgaattgcc ggtgtcgttc gaccgcctgg tcgggctgtt cgacgccgcc
    11881 atcatccggg tcgagaacca cagcttcgcc ggcctctacc gcgagtacct cgccttcgcc
    11941 acccggcgcg cactggccga cgcgcgcttc gcgccgttcg ccgacgagat cgccgccgcc
    12001 gtcggcgaga ccgccgaacg ccgcttcaac gtgctcaaca cggtcggcac cggctacggc
    12061 cgcctgaccc tgccgttcgc gcgcgagcag atccgctcgg agatcctgtg ccacggcctc
    12121 ggcgcgcact acatgttccc ggacacccgc accgtgctcg acatcggcgg ccaggacacc
    12181 aaggcgatcc aggtcgacgg caacggcatc gtcaccggct tccagatgaa cgaccgctgc
    12241 gccgccggct gcggccgcta cctcggctac atcgccgacg agatgagcat cggcgtgcac
    12301 gaactcgggc cgctggcgct gcagtcgacc aagccggtca agatcaactc gacctgcacc
    12361 gtgttcgccg gcgccgagct gcgcgagctg atggcgctgg gcgagaagcg cgaggacatc
    12421 ctcgccggcc tgcaccgcgc gatcgtgctg cgcgcgatgt cgctgctggc gcgttccggc
    12481 ggggtggagt cggaattcac cttcaccggc ggcgtcgccc gcaaccaggc ggtgaccggc
    12541 gcgctcgagc acctggtgtt cgagaactac gggaagatga cgatcaacat cagcgccgac
    12601 agcatcttca ccggcgcggt cggcgccgcc ctgttcgcca agcgcgaccc caagggcgtg
    12661 atgtcggacc tcgaagtcag caagctgccg ggcagcaagg cctgtggcct cgccgccgcc
    12721 tgccccgcct gaaggagacg ccccatgttc cataccgtcg gtatcgatgt cggttgcgat
    12781 tcggtcaaga ccagcctggt ggccttcgac gacgatggcg gcaacccgcg cctgctgttc
    12841 aaggacctcg acaagatccg caagcgcaac gtcaaggacg tggtgcgctc ggcgttcgag
    12901 cgcgcgctgc tgcgcgaggg cctgtccgaa gccgggatcg cctacatcgc caccaccggc
    12961 gagggcgagc tggtcgactt ccgcaccggg cacttcttct cgatgaccgc gcacgcgcgc
    13021 ggcgcgatct tcctggaccc cggggtacgc gcggtggtcg acgccggcgc cctgcacgcc
    13081 aaggcgatgg tggtcgacga acgctcgaag gtgatcgcct accggatgac ctcgcagtgc
    13141 gcgtccggct ccgggcagtt cctggagaac atcgcgcgct acctcggggt ggcgatcgac
    13201 gacatcggcg cgctgtcgct gcagggcgac tcgcccgagc aggtctccgg catctgcgcg
    13261 gtgctggcgg aaaccgacgt catcaacatg gtcagccgcg gcatcagcac cgccaacatc
    13321 ctgcgcggca tccacctgtc gatggccaag cgcctgatcg ggctgatgcg catgatcaag
    13381 acctccggcg acgtgctggt gaccggcggg ctggcgcgca acgccggctt cgtcgccgcg
    13441 atgcaggaac tggcggcgca ggacaagcgg ctgaaggcca acatcgtggt gcacccggac
    13501 tcggtgcacg cgggcgcgat cggcgccgcg ctgtgggcgg gctaccgcca cctgcggctg
    13561 gaggcaccgg cgctggcgca gcccgaggcc gcctgatgag cgagcacgcc gcccacgcca
    13621 tgccgatcct gcgcaacccg caggcgctgg ctgcggtcgc ccccaacatc ggcagcctgg
    13681 tgctcgacaa cgccgcccgc catgacggcg caccggcatt cgccgagcgc gtcgatgggc
    13741 gctacgccag ccacagctgg cggcagttcg gggccgacct gctgcgcgtc gccgcctggc
    13801 tgggccagcg cgagcgggtc gcgttcgtgg ccggcaacag ctacctgcgg catgtcgcgg
    13861 aactggcgac gatggccgcc ggcggcgcca gcgtgccggt gttcgccggc tacccggcgg
    13921 cgctgatggg cgagttgctg cggttctccg atgtcgagct gctggtcacc gaccagccgg
    13981 acaaggtgct ggcgctggat ccggcgtgcc tgccgcggca cctgctggtg ctcgccgagc
    14041 cgcgcccgta cgacccgtcc gcctacctcg ccggcggtgg ccggatcgac cgcatcggcg
    14101 cggtgctggc gcgcgaaccg gatgcggcaa gcctggcccg ggtcgaacgc gagctgcgcg
    14161 cggtcgccgc cgggcagacc tgcatgatca tgtacacctc gggcaccagc ggcttcccga
    14221 agggcgtgca gctgacccac cacaacctgc tcagccagca gcaggcgctg gcgcagtgct
    14281 ggcagccgca gcccggcctg cgcatgctct gctacctgcc gtggcaccac agcttcggcg
    14341 ggctgttcga gcgcttcttc gcggtcgcgt tcggcggttg cctggcgctc gacgacggcg
    14401 gcggcaggga cgtcgaccgc ctgctggaga acttcgccga aatccgcccg cacctgttct
    14461 tcagcgtgcc caagatctac caggagatcg ccgcgcgggt gcaggccagc gccggggtcg
    14521 aacgcgcgtt cttccatccc gaactcaggt tcgtgttcac cgcggcggcg ccgctgccgc
    14581 tcagtgcgtc cgacgtgttc cgcgcgcacg cggtgccggt ggtcgaaggc tggggcctga
    14641 ccgagacctc gccgtgctgc acgctgacgc cgttcagcct ggaccgcacc ccgggcgtgg
    14701 tcgggctgcc gatccccggt gtcgaggtgg cgcttgccgc cgacggcgag atcctggtgc
    14761 gcggtgccaa cgtcatgcag ggctacttcc gcaacccgga ggcgaccgcg gccgccttca
    14821 ccgacgatgg ctggttcagg accggcgaca tcggcgagtt cggcgccgag ggcgtgcgca
    14881 tccggtcgcg caaggaacgc atgttcaagc tcagcaacgg cgagaaggtg ttcccggcgc
    14941 agatcgaggc gcgcatccac gcccgctgcc agttcgtgaa gtacgcctac gtgttcggct
    15001 ccggccagcg caccccgcac ctgctggtgt tcccgaacca gcaggcgttc gagggctgcg
    15061 gcagcagcaa cgggtgcgcc cacccgaacg acgccggcca gctcgcgcat tgcctgtcga
    15121 actgcatggc cgagatcaac gcgcgcccgg ccgccggctt cgagaccatc cgccgcgcgc
    15181 tggtggtcgg ccgcgagctg agcatcgacg agaacgaact gaccccgtcg ttcaagctga
    15241 tcccgaagcg gatcgaggaa cgctacgccg actgcatcga ggcgatggaa cagggccgct
    15301 acgaacacct gccggcggac gtgttcgtgc tggacctgga ggaacgcaag tgagcatcca
    15361 ttacggcgtc atcggcaccg gcccggtcgg gcgcgtgttc gccgggctgc tgcgccaggc
    15421 cgggcatcgg gtcagcgtgc tcgcccatcg cgcgcagacc cagcagatcc tgcagcgccg
    15481 gccggtggtg gtgaagggca agctcgaggc ctatgcgcag ctgcaggacg tggtgctgga
    15541 aaccggcgac ttcgtgcgcg ccgcgcccga cgtggtcctg ctctgcacca agagcgtgga
    15601 cagcgagcac gtgctgcgca ccctgcgcga gcacgcgccg aagccaggca tgctgttcgt
    15661 gtcctgccag aacggcatcg gcaccgaacg cgtgatcgaa gccgtgttcg gccgcgagcg
    15721 cgcgctgcgg atcgtgctca acatgggctg cgacgtgtcc tgcggcaacg aggtgatcgt
    15781 caccttctgc accacccacg tgatgggccg ctcgccgcac gtggacccgg cgcggatcgc
    15841 ggcgatcgcc gccgacctca aggactccgg gctgccgctg gaactgcgcg acgactggcg
    15901 cgaggacgtg ttccgcaagg cgctgctgaa cgcctcgctc ggcagcatgt gcgcggtgac
    15961 gcgccactgc atgggctgcg tgatgggcca gccggaggcg cgcgcgatgg tcgaggacat
    16021 gctccgcgaa ggcatggcga tctgcagcgc cgagggcatc gagctcgggc ccgacttcct
    16081 cggcttcgcg atgcactacc tcgaccaggg tggcgagcac cggccctcga tcctggtcga
    16141 cgtcgagaag ggcaaggtca ccgagaacga attccactgc ggcgagctgg cccggctcgc
    16201 gcgcaagcac ggcgtggcgg caccgacgat ccacttcgtc gacaaggtgc tgcgttgcgc
    16261 ggagaacgtc tgatgcgcgg cttccgcaac gttgccgtgg tcggcgccgg caccatgggc
    16321 gcggcgatcg cgcagcattt cctgatgaag ggcctgcagg tgcacctggc cgatgccaac
    16381 caggccgggc tggcgcgtgg ccagggcctg gtcgcggcct cgctggacga ggcggtggcg
    16441 cgccgcctgc tcgatgccga cggccgcgcg gcgacgctgg cgcggctgca tccgaccacc
    16501 gacctggccg accttgccgg tgtcgacctg gtggtcgagg cggtgttcga ggatctcgac
    16561 gtcaagcgcg cgttgttcgc gcagttggaa ggcatcgtgc gcacggattg cgtgctggcg
    16621 accaacacct cctcgttccg ggtcggcgat gtcgctgccg gtctcgcgca tcccggccgc
    16681 gtgctcggca cgcactactt ctatcacgcc gccaagaaca agctggtcga gctgatcgcg
    16741 tgcgaacaca ccgcgccgga cctgctggcc gcggtcgagg cgttctatgc cggcctcggc
    16801 aagaccccga tccgcaccgc cgatgcaccc ggcttcgcgg tcaaccgctt cttcgtgccg
    16861 tggctcaacg aagcggtgcg gctgcatgcg gagggcctcg gcagcatccc cggcatcgac
    16921 cgggtcgcgc gcgaggtgtt cggggtcggc atgggcccgt tcgcgctgat gaatgccacc
    16981 ggcgtgccga tcgcgcagca cgcggccgag ggactggcgg ccacgctcgg cgacttctac
    17041 gcgccggcac cgcgcctggt cgaacaggtc gccgcgcgcg cggactggaa cctcgacgat
    17101 tgcacggtgc cgcacggtgg cgcggagcag ccgcaggtgg tccgcgaacg cctgctcgct
    17161 gccgcgctcg gttgcgccgc ggcgctggcc agcgaaggcg tgtgcagcgc caccgacacc
    17221 gacctcggcg cgcgtgccgg cctgcgctgg ccgcgcggac cgttcgagct gatggcggcg
    17281 atcggccagg acgccatcgc gggcatggtc ggcgcgctgt tcgc
//
LOCUS       DAORUK010000027        17311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_2978_length_17311_cov_18.168521, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000027 DAORUK010000000
VERSION     DAORUK010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 17311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17311
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2978_length_17311_cov_18.168521"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..589)
                     /locus_tag="PK818_03150"
     CDS             complement(167..589)
                     /locus_tag="PK818_03150"
                     /inference="COORDINATES: protein motif:HMM:NF016153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HQY81763.1"
                     /translation="MNSASILPHLNAVLNGISIVLLVVARVMIARGDIEAHRRAMLSA
                     LGVSALFLLSYIAYHVTSPIFVFRGHGVVRPVYYTLLVSHVILAAVALPLILVTARLG
                     LKRAVPRHRRWARWTWPIWMYVSVSGLAVYLMLYGIES"
     gene            857..1417
                     /locus_tag="PK818_03155"
     CDS             857..1417
                     /locus_tag="PK818_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HQY81764.1"
                     /translation="MAASPELQAVLWPVARPVAPFRMRTDRGEAFDEASFRGQWSFVF
                     FGFLQCPDVCPTTLQALRGFRRELLERDPAAARHRFLFVTVDPENDTAAGIRPYLEYF
                     DPEFVGLVGEGAELATLAGSMAAHYAKRRDGNGRTSFEHTASVMVVDPAGRVVGALPS
                     PHEPAMMARRFLALQRHVEAGNDPRR"
     gene            1677..1850
                     /locus_tag="PK818_03160"
     CDS             1677..1850
                     /locus_tag="PK818_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81765.1"
                     /translation="MSDEQRDQNAGGDDSKVPLTQQLLDNPFLLLFLGVMIPMVVYSL
                     WGVIDILTIPLAK"
     gene            1884..2423
                     /locus_tag="PK818_03165"
     CDS             1884..2423
                     /locus_tag="PK818_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81766.1"
                     /translation="MTAILPPAERLWWKHPIDRVEGTWIAIAFVWCMIMFFMMVGWHI
                     YGNQNLSTETYKTTPDAFAAKAQVMVDKYTVRSETDAETPVVAPPAGSDVYLIARLWN
                     FWPILELEKDKTYRLHLTSMDYNHGFSLQPANINIQIVPGYEHVVTVTPNQSGTFSVV
                     CNEYCGIGHHTMVGRIYVK"
     gene            2434..4134
                     /locus_tag="PK818_03170"
     CDS             2434..4134
                     /locus_tag="PK818_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQY81767.1"
                     /translation="MSHATTYRTCPRSGLQFEAQAEKLMLANAVAAVVFLLLGGILAI
                     GVVLTRWPAVHWLEADTFYQVLTAHGIDMLIFWIIFFEMAVLYFCSSTLLRCRLATPR
                     LAWAAFALMLIGAIMNNYAVYDGASSVMMTSYVPMMAAPTFYLGLILFAVGALVGCFI
                     FLGTLVVAKRDRTYEGSVPLVTFGAITACIIAVFTIVSGAIILVPTFLLSVGLIDAVD
                     PLIYRTIWWAFGHSSQQINVAAHISIWYAVAAIVFGAKPMSERVSRGAFLLYILFLQL
                     ASAHHLLADPGMSTEWKVVNTSYFMYFAVLASMIHGLTIPGAIEVAQRKKGYNKGLFE
                     WLRKAPWGNPAFSGVFISIIGFGFLGGISGVMMGTEQLNMIIHNTIYVPGHFHATVVV
                     GTTLSFMALTYFLIPVLFKREMISPVLARWQPYLFGLSMYFFCLVMMGAGTLGVSRRH
                     WDMAFSGAALAYEWPGTAYLLMGLVGIAGLAAIVGGGIYIYVTVGSLLWGKKLDGGAT
                     SSAPTPIPPTAPTHDVQTYGSMGFAAPGTFVLAMVFLVSFVLYYFVNWKYLSTLWGLS
                     "
     gene            4226..5122
                     /gene="cyoE"
                     /locus_tag="PK818_03175"
     CDS             4226..5122
                     /gene="cyoE"
                     /locus_tag="PK818_03175"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HQY81768.1"
                     /translation="MTTTSSIAGTSFQIRARTIAGLFKLRIGSLIMITALAGMVVTPG
                     GDLGWTERMVLALSVLVASASAGAFNQYYERDSDRLMVRTRERAFASGAVAAGPGWLW
                     LMAAMLAGSVAAAAWVLNPVSALFVFLGAFFYACVYTVWLKRRSAWNIVIGGLAGSFA
                     VLAGAAAVDPAIGPQGLILALVLLLWTPPHFWSLAIAYQREYGAAGIPMLPVVVGIPR
                     AARIVLLSTIALVLASLLPMFHGAGPAYLAGAAIGGVYFLIKARALDRKPDRGTAIGS
                     FLASLLQLSLLLAGACVDALLK"
     gene            5128..6051
                     /locus_tag="PK818_03180"
     CDS             5128..6051
                     /locus_tag="PK818_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HQY81769.1"
                     /translation="MAGAPGHRVCRPRSRVLRAGLFALLALFAAVPGSPTRAGPAPGQ
                     SQVVQSGASAAAAPAAFALDQEAAFALSRSVIGTRPVARVMRDQDGREVSLAEFRGKP
                     LLVNFIYTGCFQVCPTTSRALNGAVKAMRERFGSDQFNIVSIGFDQPTDSPASLRIYA
                     GQQRIDVSNWRFLSPRGEDVPALARDFGFSYTRSPIGFDHTLQVSILDAEGRIHRQVY
                     GDAFGADSLGEPLKQLIAGTMLADTQGLDDLIGRVRILCSVYDPLTGKYRTDYTLYVE
                     LAGGITFVLAMLWFALSEWRNRRRSRRMAMG"
     gene            6069..6587
                     /locus_tag="PK818_03185"
     CDS             6069..6587
                     /locus_tag="PK818_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81770.1"
                     /translation="MKMPSTRQAATWGAQGVLYAAFAGFLALFSSWPDFRSLQPDEGL
                     ITISIAHHGQRLQPCREQSLEELARLPPNMRVASRCPRERAPLLVEVDIDGTTVLRQR
                     AEASGLSRDGAGKLYRRMPMPVGVHQVRVRLRDSNRSEGFDYVREEQVELRPAEILVV
                     DFDSEQREFTFK"
     gene            6584..8020
                     /locus_tag="PK818_03190"
     CDS             6584..8020
                     /locus_tag="PK818_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQY81771.1"
                     /translation="MSRPQDIDPRTVAPPLAAVASGHDYVLEPDAGVDRTLARSWLGL
                     GLFALVGSGVFSILLVLSRSPYINQWLPVPSFFRVALVAHVDLSVLVWFVSVAGLLWT
                     VAGGRRASALGWSALALCGAGTAIMALAPFVGRGEPIMANYVPVLDGAMFLAGLSVFA
                     IGSAALVLWSLWAGPRPGLVADGVAALRFGLSAAAVAAAVALLAFAWSLVLVPMDLDS
                     KAYYEILFWGGGHALQFVWTLLMLVAWLWLASATGARVPMSPRLVLAMFGLALVGVFA
                     TPYAYLAHDIASVEHRHLHTWAMRLGGGLAVLPIGLATVLALVRRPTPLAAAAQPLQA
                     ALVASIVLFAVGGVIGIFISGSNVKIPAHYHGSIVGVTLALMGLVYLLLPKLGYGTPS
                     GRMARWQPTLYGAGQLMHIIGLVWSGGYGVQRKVAGAEQVLRTPPEIAGMALMGLGGA
                     VAIVGGLLFVVVVAKAVRQPSGNGVHGS"
     gene            8017..9954
                     /locus_tag="PK818_03195"
     CDS             8017..9954
                     /locus_tag="PK818_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQY81772.1"
                     /translation="MIKAIQRLLRWCFMRVEALFNRAFGDRINPFYQLGAIGFFLFWL
                     VAGSGLYLYAFFDTSVTGAYTSVEALTHGQWYFGGILRSVHRYASDALVLVMFIHMLR
                     HFAFDHLRGYRWFAWVTGVGLIWFMYICGINGYMLPWDRLAQFVTVASFEWLDWLPGM
                     GGSLVRNFMYADSVDDRFFSLLAFVHIGAPLLTLLLMWVHIQRVPKASTTPPRAIAIG
                     ICLMLVVLSLLKPALSQGGPADLGTAVAEIGLDWFYLGLLPLIYRMPMWQVWALAAAV
                     TVLLAALPWVPLRRKDARGELRIEMHPGGHQVSARPGETLLDAGLRAGLVLPYDCRNG
                     GCGVCVCTVLQGQVDQGAYQPAVLTAAMRARGKALMCCATALDEVALEVDVHSLEPGM
                     ATPVLSAEARVAGLQLLSEDVMQVLLTLPGGERLDFIAGQYLNIVLEDGQRRAFSFAS
                     PPHRGMPIELHIKLVPDGRFTPRVFNSLKVGDTLRIEGPFGRFTLSESDKPIILIATT
                     VGFAPVKSILEDAFHRGIKRPMQLYWGARHRRDLYQLELAEGWARDYPNFRLEAVLSG
                     APDGSWAGRSGRASAAILEDHPDLSGHEVYACGSVRMVEDVVPTFLARGLSENACFSD
                     AFSSGYVGEPTCCDAAGPAAT"
     gene            complement(9978..12815)
                     /locus_tag="PK818_03200"
     CDS             complement(9978..12815)
                     /locus_tag="PK818_03200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQY81773.1"
                     /translation="MRAVLRVAWMVVALCLAQAASALDPRLAISQYQLSQWHSADGLP
                     HSSISALFEDRDGFIWVGTYRGAARFDGKRFLPLAELIGEPLDSDLVETIGQTADGVL
                     WFGGTYRSLVLLAPDGRVRRYGAGQGLPDRQITAVFTDAGGQRWLGTRSGLLRVDGPW
                     DRLRFSADLPQIVWQLVQWQGDLYAASERGVWRRRGNTWEHLFADTALANVHVWALEP
                     DGGRLWIGYRGGLASFDGRALQLLAEANGLPHPVVRALARDRDGNLWIGTSGAGIARL
                     AAGRFDTLGDRDGGVGGVTWGMVLGRDGTLWVASASGLSRLKNASVRNYGRPEGLRSA
                     MVWGTLDDRRGGRYVAFNGEGLAHVDVTGQARHFGAPNGPPGAGIVISMAHDGDDLLL
                     ATFSGLYRFHDGRFTREPAVPADRVHAIHVLAQDDVLLGMHGGLWRLRGQRLARIPSS
                     LREPVLRMRTEADGSLLVAAGRDGAWRLQGDRMSALASRPGTEVRDVFRASDGRIYAA
                     GLGLFVIDRGRLLPLLPINRTQVGQMHAITESADRKLWINSNLGVIRVALDQVDAWLG
                     HRRQGLPFQVVDETHGMRSSEGNGGQNSADLDRDGKLWLATTAGVAVIDTRADLEPPL
                     PPLQVHFERRLEDGQGRFAGATQAVTAGVRRLQVEYTVASPAWADALGFEYRLLPLDR
                     DWVDNGSLRSIAMGRPPPGDYRLDVRARRGGTTGETASLEFSVAAFWWQHRWVQRSAL
                     LATVLLTGLLVWWRVLALRRQRDVLERQVQVRTTELAAANAQLADAARRDFLTGIGNR
                     RCFTERLNALWALTPEIALAVVDVDHFKAYNDRLGHLQGDQCLRAIGATLRTLEGADI
                     EVFRVGGEEFALLAAHAAADRIGAVLGDLRRALATAALAHPGSDVSPWVTLSAGHALR
                     SDADQRPEDLYARADAALYRAKAEGRDRIVS"
     gene            12959..14005
                     /locus_tag="PK818_03205"
     CDS             12959..14005
                     /locus_tag="PK818_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQY81774.1"
                     /translation="MQEPARPKRKTKATSLDIAHRAGVSQATVSRVLRGSPLVNAETR
                     KRVEDAVRELNYKVDRHASSLRTQRAGTLALLLFEDPTPDDSHINPFFLSMLGSITRA
                     CARHGHDLLVSFQQLSDDWHADYEDSMKADGLILLGYGDYLAYESKLSHLVAQGTHFV
                     RWGAVLPGQPGLSIGCDNAEGGALAGRHLIASGRKKIAFLGDASTHYPEFFARYCGCD
                     AALREAGLRMNPALQVDATSTEQSGHDAAKVLIARGLAFDAVFAASDLIAIGAMHALK
                     EHGLRVPEDVAVVGFDGTPMARFSNPPLTTVVQDTSKAGELLVDTLLQLVRDEPAHSI
                     TLAPTLQVRQSSGG"
     gene            complement(14027..15748)
                     /locus_tag="PK818_03210"
     CDS             complement(14027..15748)
                     /locus_tag="PK818_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019337082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQY81775.1"
                     /translation="MRIATTPLAAAFALAFAATGCASTPAPAPAPAAGAAPAYYGTLE
                     PFASDAVYFLLTDRFVNGDASNDHRDQGKARGEAFFTFDRPVPGAPAGRHANVGYLGG
                     DFKGVADHAAYIRDMGFGAVWITPIVDNPDEAFTGGEPVKWEGFWTDGGKTGYHGYWG
                     VDFYQLDEHLPSPGLDFRGLANVLRAHGLKTVLDIVANHGSPAYTMPVDQPKFGEIRD
                     RDGTLLADHMNLAPEKLDPQNPLHAFYNSKPGIAQLSDLNENDPRVLPYLLGAYEQWI
                     EQGADAFRIDTIGWLPHSFWHEFSRRIRDRHPGFFMFGEAFDYEAGKIAEHTLPGNAN
                     VSVLDFPLKQALANLFEKPGGDFASLAAPLHLTGGPYSNPYELMTFYDNHDMPRMKAS
                     DNGFIDAHNWLFTARGIPVIYYGSEIGFERGTAEHAGNRNYFGSERIEAAKGHPIQQA
                     LTRIARVRAASPALQRGLQVNIELQGDRAAFYRAYQHAGRQQLALVLLNKGDAPQRFE
                     IASMLQAGRWRSALDGSVVEVAAHGALAADVAAHGVQVFLLDAEISEPSLEAALAAAM
                     DDARRPR"
     gene            complement(15762..17252)
                     /locus_tag="PK818_03215"
     CDS             complement(15762..17252)
                     /locus_tag="PK818_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY81776.1"
                     /translation="MASNPSARKPQLSFWQIWNMCFGFLGIQFGFALQNANVSRIFQT
                     LGAEMDSLPGLWIAAPLTGLVVQPIIGYLSDRTWTGLGRRRPYFLVGAVLSSIALLFM
                     PDSPTLWIAAGLLWVLDASINISMEPFRAFVGDQLPVSQRAKGYAMQSFFIGIGSVIA
                     SMLPWMLEKWGVANTADAGQVPDTVRYAFYAGAGVLLGAITWTVLRTREYPPDALHAY
                     DEAAPLDRAPLQLARATRFGLLWLGIGALGLALVALQGLPRELYLLGALLAAYGIALL
                     ALNVVGPDNGFAQVMTDIYAMPTAMRRLAIVQFFSWFALFAMWIYTTAAVTSVYYGST
                     DTASAAYNTGANWVGVLFAAYNGFAALAAIAIPAMASRLGLRMSHLVNVSLGGLGLAS
                     LPFMPNEDWLLLSMAGVGFAWASILSLPYALLSDSVPARKMGVYMGIFNFFIVIPQLV
                     AVATLGYLLKHLFADDPSKILVLGGASMVVAGLCVLRVPEPAAHAA"
BASE COUNT         2589 a         5830 c         6012 g         2880 t
ORIGIN      
        1 ctccgggcgc accaaatgcg aaaggccgat gggaaaccat cggccttttt catttgccgg
       61 gattgcatcg agaacgttcg cggcaatccc gttccgttgc gatgcaagcc aggtcgcacg
      121 gccgctggaa cctacctgca ccatgcaggc aggcagccat ctccgatcag ctctcgattc
      181 cgtacagcat caggtacacc gcgagtccgg acaccgagac atacatccag atcggccagg
      241 tccagcgcgc ccagcgacgg tggcggggca cggcccgctt caggcccagc cgcgcggtca
      301 ccaggatcag cggcagggca accgcggcca ggatcacgtg gctgaccagc agcgtgtagt
      361 agacgggccg gaccacgcca tgtccccgga aaacgaagat gggcgaggtg acgtggtagg
      421 cgatgtacga caacaggaac agggcggaca cgcccagcgc cgacagcatc gcgcgccggt
      481 gcgcctcgat gtcgcccctg gcgatcatca cccgcgccac caccagcagg acgatggaaa
      541 tcccgttcag gacggcattc aggtgcggca ggatgcttgc ggaattcatc gggatggcgt
      601 ggctggcgct ggctgcggag ggcatcttcc ggcatggcca acccccgcgc aatccgttgg
      661 cgcgactgac ccaggtcaat gcggcgcacc cccgtcgcga tgcatcctgc ggcggacagg
      721 tgcaacctgc gccgctgccg agcacactcc gatgcggaca actccattgc gctggatcgc
      781 gccgctggtc gctaccgttg cactcgccgc cgtgctttgg gcatggatgc aggccccgcc
      841 gagggcggcg gatgccgtgg cggcctcacc cgaactgcag gccgtgctgt ggccggtcgc
      901 gcgcccggtg gcaccgttcc ggatgcgcac cgatcgtggc gaagccttcg atgaagcgtc
      961 cttcaggggg caatggagtt tcgtgttctt cgggttcctg cagtgcccgg acgtgtgccc
     1021 gaccaccctg caggccctgc gggggttccg ccgcgaattg ctcgagcgcg atcccgccgc
     1081 cgccaggcac cgcttcctgt tcgtgaccgt ggatccggag aacgacaccg ccgcgggcat
     1141 caggccttac ctggagtact tcgacccgga gttcgtcggc cttgtaggcg agggcgcgga
     1201 gcttgccacg ctggccggct cgatggcggc gcactacgcc aagcggcgcg acgggaacgg
     1261 ccgcaccagc ttcgagcaca ccgcctcggt gatggtggtg gacccggccg gccgggtggt
     1321 gggggcgctg ccctcgccgc acgagccggc gatgatggcg cggcggttcc tcgcgctgca
     1381 gcggcatgtc gaagccggca acgacccgcg ccggtgattg cgcgcggcct gcacgccgcc
     1441 ggcgcggggc tgtatattca ggggcggttg atccagatca aggacggcca ccggcaccgc
     1501 cggtaaacat ggtgcggctc gggatttcgc catggatccg agccgatgac ctggggggcc
     1561 cgcccccgtt ccggcttgct gccggggccg gggcaggaac tggtcgattg cccaatggcg
     1621 ggatccggcg tcggcaaggg gacattgcat gcgctcgcac gatcaggaga gattccatga
     1681 gtgacgaaca gcgcgaccag aacgcagggg gcgacgattc caaggtcccc ctgacgcagc
     1741 agttgctgga caacccgttc ctgctgctgt tcctcggggt gatgatcccg atggtcgtct
     1801 acagcctgtg gggtgtcatc gacatcctca ccatcccgct tgcgaagtaa ttccgcagcc
     1861 gcgccaactc ccgggggaac accatgaccg caattttacc gccggccgaa cgactgtggt
     1921 ggaagcaccc gatcgatcgc gtcgaaggca cctggatcgc gatcgcgttc gtgtggtgca
     1981 tgatcatgtt cttcatgatg gtgggctggc acatctacgg taaccagaac ctctccaccg
     2041 agacctacaa gacgaccccc gacgccttcg ccgcgaaggc gcaggtgatg gtcgacaagt
     2101 acaccgtgcg cagcgagacc gacgccgaga cgccggtggt ggcgcccccc gcgggcagcg
     2161 acgtctacct gatcgcgcgg ttgtggaatt tctggccgat cctggagctg gagaaggaca
     2221 agacctatcg cctgcacctg acgtcgatgg actacaacca cgggttttcg ctgcagccgg
     2281 cgaacatcaa catccagatc gtccccggat acgagcacgt ggtgacggtc acgcccaacc
     2341 agagcggaac gttctcggtg gtctgcaacg aatactgcgg gattggccat cacacgatgg
     2401 tcggccgcat ctacgtcaag taaggggcgc ccaatgtcgc acgcaaccac ctatcggacc
     2461 tgtccgcggt ccggcctgca gttcgaggcg caagcggaaa agctgatgct cgcgaatgcg
     2521 gtcgcggcgg tcgtgttcct gctgctgggc ggcatcctcg cgatcggcgt ggtgctgacc
     2581 cgctggccgg cggtgcactg gctcgaggcc gacaccttct accaggtgct gaccgcgcac
     2641 ggcatcgaca tgctgatctt ctggatcatc ttcttcgaga tggcggtgct gtacttctgt
     2701 tcgtcgacct tgctgcgttg ccgactggcc acgccaaggc tggcatgggc cgcgttcgcg
     2761 ctgatgctga tcggcgcgat catgaacaac tacgccgtgt acgacggcgc atccagcgtg
     2821 atgatgacgt cgtacgtgcc gatgatggcc gcgcccacgt tctacctcgg cctgatcctg
     2881 ttcgcggtcg gcgccctggt cggatgcttc atcttcctgg gaaccctggt cgtcgccaag
     2941 cgcgaccgca cctacgaggg ctcggtcccg ctggtgacct tcggcgcgat caccgcctgc
     3001 atcatcgcgg tattcaccat cgtgtccggt gcgatcatcc tggtgccgac cttcctgctg
     3061 tcggtcgggc tgatcgacgc ggtcgacccg ctgatctacc gcaccatctg gtgggcgttc
     3121 ggccactcct cgcagcagat caacgtcgcc gcccacatct cgatctggta cgcggtcgcg
     3181 gcgatcgtgt tcggcgccaa gccgatgtcc gagcgggtca gccgcggcgc gttcctgctc
     3241 tacatcctgt tcctgcagct agcgagcgcg caccacctgc tggccgaccc gggcatgagc
     3301 accgagtgga aggtcgtcaa caccagctac ttcatgtact tcgcggtgct ggcctcgatg
     3361 atccatggcc tcaccatccc cggtgcgatc gaagtcgcgc agcgcaagaa gggctacaac
     3421 aagggcctgt tcgaatggct gcggaaggcg ccgtggggca acccggcctt ctccggcgtg
     3481 ttcatctcga tcatcgggtt cggcttcctc ggcggcatct ccggcgtgat gatgggcacc
     3541 gaacagctca acatgatcat ccacaacacc atctacgtcc ccgggcattt ccatgccacc
     3601 gtggtcgtcg gtaccaccct gtcgttcatg gcgttgacct acttcctgat cccggtcctg
     3661 ttcaagcgcg aaatgatcag cccggtgctg gcgcgctggc agccgtacct gttcggcctg
     3721 tcgatgtact tcttctgcct ggtgatgatg ggcgcgggta ccctcggcgt gtcgcgccgg
     3781 cattgggaca tggccttcag tggcgcggcg ctggcctacg agtggccggg caccgcctac
     3841 ctgctgatgg gcctggtcgg gatcgcgggc ctggccgcga tcgtcggcgg cggcatctac
     3901 atctacgtca ccgtcggttc gctgctgtgg ggcaagaagc tggacggcgg cgccaccagc
     3961 agcgcgccga cgccgatccc gccgaccgcg cccacccatg acgtgcagac ctatgggtcg
     4021 atgggcttcg cggcgccggg gaccttcgtg ctggcgatgg tgttcctggt ctcgttcgtc
     4081 ctgtactact tcgtgaactg gaagtacctg tcgacgctgt ggggcttgag ctgagtccag
     4141 acgcgtcgat cgcccccgcg ccgcgccggc aacaggcgtc cgcgggggtt ccgttccggc
     4201 agcgtcggca accggggtgc ctgcaatgac aacgacgtcc tcgatcgccg gcaccagttt
     4261 ccagatccgc gcacgcacca tcgccggcct gttcaagctg cgcatcggca gcctgatcat
     4321 gatcaccgcg ctggcgggga tggtggtcac ccccggcggg gacctggggt ggaccgagcg
     4381 gatggtgctg gcgctgtcgg tactggtggc gtcggccagt gccggtgcat tcaaccagta
     4441 ttacgagcgc gactcggatc ggctgatggt ccgcacccgc gagcgcgcct tcgccagtgg
     4501 cgcggtcgcg gccggcccgg gctggctgtg gctgatggcg gcgatgctgg cgggatcggt
     4561 ggcggcggcg gcgtgggtgc tgaacccggt gtcggccctg ttcgtgttcc tcggcgcgtt
     4621 tttctatgcg tgcgtctata cggtctggct gaagcggcgc tcggcctgga acatcgtcat
     4681 cggtggcctg gccggcagct tcgccgtgct ggctggcgcc gcggcggtgg acccggcgat
     4741 cggcccgcag ggcctgatcc tggcgttggt gttgctgctg tggacgccgc cgcatttctg
     4801 gagcctggcg atcgcctacc agcgcgagta cggcgcggcc gggataccga tgttgccggt
     4861 cgtggtcggg attccgcgcg cggcgcggat cgtgctgttg tcgaccatcg cattggtgct
     4921 ggcatcgctg ctgccgatgt tccacggcgc cgggccggcc tatttggcgg gtgccgcgat
     4981 cggtggcgtc tacttcctca tcaaggcccg cgcgctggac cgcaagcccg accggggcac
     5041 ggcgatcggc agcttcctgg cgtcgctgct gcagctgtcc ctgctgctgg cgggtgcctg
     5101 cgtcgacgcg ctgttgaagt gacggcaatg gctggtgcgc cgggccatcg tgtctgccgg
     5161 ccgcgttcgc gcgtgttgcg ggcagggctg ttcgcgctgc ttgcgctctt cgccgcggtg
     5221 cccggcagtc ccacccgtgc aggcccggcc cccggccagt cgcaagtcgt gcagtccggc
     5281 gcgagcgcgg ccgccgcgcc ggctgcgttc gcgctggacc aggaagccgc gttcgcgctc
     5341 agtcgttcgg tcatcgggac ccggccggtt gcccgcgtca tgcgcgacca ggacggacgc
     5401 gaggtctcgc tggcggagtt ccgcggcaag ccgttgctgg tcaacttcat ctacaccggc
     5461 tgcttccagg tctgcccgac cacctcgcgc gcgctcaacg gcgcggtcaa ggcgatgcgc
     5521 gagcgcttcg gcagcgacca gttcaacatc gtcagcatcg gcttcgacca acccaccgac
     5581 tcgccggcct cgctgcggat ctatgccggg cagcagcgga tcgatgtctc caactggcgc
     5641 ttcctgagcc cacgtggcga agacgtgccg gcgctggcgc gtgacttcgg cttcagctac
     5701 acgcgctcgc cgatcggctt cgaccacacc ctgcaggtca gcatcctcga tgcggaaggg
     5761 cgcatccacc ggcaggtgta cggcgacgcc ttcggcgccg attcgctcgg cgagccgctg
     5821 aagcagctga tcgcgggaac catgctggcg gacacccagg gcctggatga cctgatcgga
     5881 cgggtccgca tcctgtgttc ggtctacgac cccctcacgg ggaagtaccg gaccgactac
     5941 acgctctacg tcgaactggc gggcggcatc accttcgtgt tggcgatgct atggttcgcg
     6001 ctctcggaat ggcgcaaccg ccgacgttcg cggcggatgg cgatgggctg accgatggag
     6061 acatggacgt gaagatgcca agcacccgcc aagccgccac ctggggcgcc cagggcgtgc
     6121 tctacgccgc gttcgcgggg ttcctcgcgc tgttttcctc gtggccggat ttccgttcgc
     6181 tgcagccgga cgagggactg atcaccatca gcatcgccca ccacgggcag cggttgcagc
     6241 cctgccgcga acagtcgctc gaggaactgg cgcggctgcc gcccaacatg cgggtggcgt
     6301 cgcgctgtcc gcgcgagcgc gcgccgctgc tggtcgaagt ggatatcgac ggcaccaccg
     6361 tgctgcgcca gcgtgccgag gcgtccggcc tgtcgcgcga cggcgcgggc aagctttacc
     6421 ggcggatgcc gatgccggtc ggcgtgcacc aggtccgcgt gcgcctgcgc gacagcaacc
     6481 gcagcgaggg cttcgattac gtccgcgagg agcaggtcga gctgcgtccc gcggaaatcc
     6541 tggtggtgga cttcgattcc gagcagcggg aattcacctt caaatgagcc gaccgcagga
     6601 catcgacccg cgtaccgtgg cgccgccact ggctgcagta gccagtggcc atgactatgt
     6661 gctggaaccg gacgccggcg tggaccggac cctggcccgc agctggctcg ggctggggct
     6721 gttcgcgctg gtggggtcgg gggtgttctc gatcctgctg gtgttgtcgc gttcgcccta
     6781 catcaaccag tggttgccgg tcccgagttt cttccgggtg gcgctggtgg cgcatgtcga
     6841 cctgtcggtg ctggtctggt tcgtctccgt cgccggcttg ctgtggaccg tggccggtgg
     6901 ccgccgggcg tcggcgctgg gctggtccgc gctcgccctg tgcggcgccg ggaccgcgat
     6961 catggcgctg gcgccgttcg ttggccgcgg cgagccgatc atggcgaact acgtgccggt
     7021 gctcgatggc gcgatgttcc tggccggatt gtcggtgttc gcgatcggca gtgcggcgct
     7081 ggtgctgtgg agcctgtggg ccgggccgcg gcccggcctg gtggcggatg gcgtcgccgc
     7141 gttgcgcttc gggctgtcgg cagcggcggt ggccgcggcg gtcgccctgc tggcgttcgc
     7201 ctggtcgctg gtgctggtgc cgatggacct cgacagcaag gcctactacg agatcttgtt
     7261 ctggggcggc ggccatgccc tgcagttcgt ctggaccctg ctgatgctgg ttgcctggct
     7321 gtggctggcg agcgccaccg gcgcgcgggt cccgatgagc ccgcggctgg tgctggcgat
     7381 gttcgggctg gcgctggtgg gcgtgttcgc cacgccgtat gcctacctgg cccacgacat
     7441 cgcctcggtc gagcatcgcc atctccacac ctgggcgatg cgcctgggcg gcggcctggc
     7501 ggtcctgccg atcggcctgg ccacggtgct tgcgctggtg cggcggccaa ccccgctggc
     7561 cgccgcggcg cagccgttgc aggcggcact ggtcgcctcg atcgtgctgt tcgcggtcgg
     7621 cggcgtgatc ggcatcttca tcagcggcag caacgtcaag attcccgcgc actaccacgg
     7681 gtcgatcgtc ggggtgaccc tggccctgat ggggctggtt tacctgctgc tgccgaagct
     7741 cggttatggc acgccgtccg ggcggatggc gcgctggcaa ccgacgctgt acggcgccgg
     7801 acagctgatg cacatcatcg ggctggtgtg gtcgggcggc tacggggtcc agcgcaaggt
     7861 ggccggtgcc gagcaggtcc tgcgcacgcc cccggagatc gccgggatgg cgctgatggg
     7921 gcttggcggc gcggtcgcga tcgtcggcgg cctgctgttc gtggtggtgg tggcgaaggc
     7981 ggtgcggcag ccgtccggaa atggagttca cggatcatga tcaaggccat ccaacgcctg
     8041 ctgcgctggt gcttcatgcg cgtcgaggcg ctgttcaacc gcgcgttcgg cgaccgcatc
     8101 aacccgttct accaactcgg cgcgatcggc ttcttcctgt tctggctggt cgccggcagc
     8161 gggctgtacc tgtacgcctt cttcgatacc agcgtgaccg gggcgtacac ctcggtggag
     8221 gcgttgaccc acggccagtg gtacttcggc ggcatcctgc gcagcgtgca ccgctacgcg
     8281 tcggacgcgc tggtgctggt catgttcatc cacatgctgc ggcatttcgc cttcgaccac
     8341 ctgcgcggct accgctggtt cgcatgggtc accggggtcg ggctgatctg gttcatgtac
     8401 atctgcggca tcaacggcta catgctgccg tgggaccggc tggcgcagtt cgtcaccgtc
     8461 gccagcttcg agtggctgga ctggctgccc ggcatgggtg gcagcctggt gcgcaacttc
     8521 atgtacgcgg acagcgtcga cgaccgcttc ttctcgctgc tggcgttcgt gcacatcggc
     8581 gcgccgctgc tgaccctgct gttgatgtgg gtgcacatcc agcgggttcc caaggccagc
     8641 accacgccgc cgcgcgcgat cgcgatcggc atctgcctga tgctggtggt gctgtccctg
     8701 ctgaagccgg cgctgagcca gggcgggcct gctgacctcg ggaccgcggt cgcggagatc
     8761 gggctggact ggttctacct cggcttgctg ccgctgatct accggatgcc gatgtggcag
     8821 gtgtgggcgc tggcggcggc ggtcaccgtg ctgctggcgg cgctgccgtg ggtgccgctg
     8881 cggcgcaagg atgcacgcgg cgagctgcgc atcgaaatgc atcccggcgg ccaccaggtg
     8941 tccgcgcggc cgggcgaaac cctgctggac gcgggcctgc gcgcgggcct ggtgctgcct
     9001 tacgactgcc gcaatggcgg ctgcggcgtg tgcgtgtgca cggtgctgca gggccaggtc
     9061 gaccagggtg cgtaccagcc ggcggtgttg accgcggcga tgcgcgcgcg cggcaaggca
     9121 ttgatgtgct gcgccaccgc gctggacgaa gtggcgctgg aagtcgacgt gcattcgctg
     9181 gaacccggca tggcgacgcc ggtgctgagc gcggaggcaa gggtcgccgg cctgcagttg
     9241 ctctccgagg acgtgatgca ggtgctgctg accctgccgg gcggcgagcg gctcgacttc
     9301 atcgccgggc agtacctcaa catcgtgctg gaggacgggc agcgccgcgc gttctcgttc
     9361 gccagcccgc cgcaccgtgg catgccgatc gagctgcaca tcaagctggt gccggatggc
     9421 cgcttcacgc cgcgggtgtt caactcgctg aaggtcgggg acaccctgcg catcgagggg
     9481 ccgttcggac gcttcaccct cagcgagagc gacaagccga tcatcctgat cgccaccacc
     9541 gtcggtttcg ccccggtcaa gagcatcctc gaggatgcct tccatcgcgg catcaagcgg
     9601 ccgatgcagc tgtactgggg cgcgcgccat cggcgcgacc tctaccagct ggaactggcc
     9661 gagggctggg cccgcgacta cccgaacttc cggctcgagg cggtgctgtc cggtgcgccc
     9721 gatggcagct gggccggccg cagcggccgc gccagcgcgg cgatcctgga agaccatccc
     9781 gacctcagcg gccatgaggt gtatgcgtgc ggctcggtgc ggatggtgga ggacgtggtc
     9841 ccgacgttcc tggcgcgcgg cctgagcgaa aacgcctgct tctcggatgc gttctccagc
     9901 ggctacgtcg gcgaaccgac ctgctgcgat gctgccggtc ccgctgccac ctgacacggg
     9961 tcagcgggcg tcgatgttca actgacgatg cggtcgcggc cttcggcctt ggcccggtac
    10021 aaggcggcat cggcgcgcgc atacaggtct tccgggcgct ggtcggcgtc gctgcgcagc
    10081 gcatgcccgg cactcagcgt gacccacggc gagacatcgc tgcctggatg cgccaacgcg
    10141 gcggtggcaa gtgcccgccg caggtcgccc aacaccgcgc cgatacggtc agcggcggca
    10201 tgggcggcga gcaacgcgaa ctcttcgcca ccgacgcgga agacctcgat gtcggcaccc
    10261 tcgagggtcc gcaacgtggc gccgatggcg cgcaggcact ggtcgccctg caggtgacca
    10321 aggcggtcgt tgtaggcctt gaagtggtcg acatcgacca ccgccagcgc gatttcgggc
    10381 gtcagcgccc atagcgcatt cagccgctcc gtgaagcagc gacggtttcc gatgccggtc
    10441 aggaaatcgc ggcgtgcggc atccgccagt tgtgcgttcg ccgccgccag ctcggtcgtg
    10501 cgcacctgca cctgtcgctc aagcacgtcg cgttgccgac gcaaggcaag cacccgccac
    10561 cagaccagca gtccggtcag cagcacggtg gccaagaggg cgctgcgctg cacccagcga
    10621 tgctgccacc agaacgccgc caccgagaac tccaggctcg cagtctcccc ggtagtgcca
    10681 ccacgccgcg cgcgcacgtc cagtcgatag tcgcctggcg gtggtcggcc catggcgatg
    10741 ctgcgcaggc tgccgttatc aacccagtcg cgatcgagtg gaagcagccg gtactcgaag
    10801 ccgagtgcgt cggcccacgc cgggctggcg accgtgtact ccacctgcag gcggcgcacg
    10861 cccgccgtca ccgcttgcgt ggctcccgcg aagcgcccct ggccatcttc cagccgccgc
    10921 tcgaaatgca cctgcagggg cggcagcggg ggctccaggt cggcccgggt gtcgatgacg
    10981 gccaccccgg cggtggtcgc caaccagagt ttcccgtcgc gatcgaggtc ggcggagttc
    11041 tggccgccgt tgccctcgct gctgcgcatg ccgtgggtct cgtcgaccac ctggaagggc
    11101 aggccttgcc ggcgatggcc aagccaggcg tcgacctggt ccagtgcgac ccggatcacc
    11161 cccaggttgg agttgatcca caacttccgg tccgcgcttt cggtgatcgc gtgcatctgc
    11221 ccaacctggg tccggttgat cggcagcagc ggcagcaacc tcccgcgatc gatcacgaac
    11281 agccccaggc cagcggcata gatccggcca tcgctggcgc gaaagacgtc gcgaacctcc
    11341 gtcccgggcc tgcttgcgag agccgacatc cgatcgccct gcaggcgcca ggcgccgtcg
    11401 cggcccgcgg cgaccagcag gctgccgtcg gcctcggtgc gcatgcgcag gaccggttcc
    11461 cgcaagctcg acggaatccg ggcgaggcgc tgcccacgca gccgccagag cccgccatgc
    11521 atgcccagca gcacatcgtc ctgcgccagg acatggatgg catggacacg atccgccggc
    11581 accgccggct cgcgggtgaa gcgcccatca tggaaccggt agagtccgga aaacgttgca
    11641 agcaacagat cgtcgccgtc gtgggccatg gatatcacga tcccggcgcc cggcggtccg
    11701 ttcggcgcgc cgaagtgcct tgcctgtccg gtgacatcga cgtgggccag gccctcgcca
    11761 ttgaaggcga catacctgcc gccgcgacgg tcgtcgagcg tgccccagac catggccgaa
    11821 cgcagccctt cgggccgccc gtagttgcgc acgctggcat tcttcaggcg gctcaggccg
    11881 ctggcgctgg ccacccacag ggtgccgtcg cgccccagca ccatgcccca ggtcacgccg
    11941 ccgacaccgc catcgcggtc acccagcgtg tcgaagcggc ctgcagccag gcgcgcgatg
    12001 ccggcccccg aggtgccgat ccagagattg ccatcgcggt ccctggccag cgcgcgcacc
    12061 accggatgcg gcaggccgtt ggcctcggca agcagctgca gggcgcgccc gtcaaaactc
    12121 gccagtccgc cgcgatagcc gatccagaga cgcccgccgt ccggctccag cgcccacaca
    12181 tgcacgttgg ccagggccgt gtcggcgaac aggtgttccc aggtgttgcc gcgccgacgc
    12241 cagacgccgc gctcgctggc ggcgtacaga tcgccctgcc actgcaccag ttgccagacg
    12301 atctgtggca ggtcggcgct gaaacgcagg cgatcccagg ggccgtcgac gcgcagcaat
    12361 ccggatcgag tgcccagcca gcgttgcccc ccggcatcgg tgaagacggc ggtgatctgc
    12421 cggtccggca atccctgccc ggcgccgtaa cggcgcaccc gtccatccgg tgcgagcagg
    12481 accaggctgc ggtaggtgcc accgaaccag agcacgccat ctgcggtctg gccgatggtc
    12541 tcgaccaggt ccgaatcgag cggctcgccg atcaactccg ccagtggcag gaagcgcttg
    12601 ccgtcgaacc gggccgcgcc gcgataggtg ccgacccaga tgaagccgtc gcggtcctcg
    12661 aacaacgcgc tgatcgaact gtgcggcaag ccatccgcgg aatgccattg gctgagctgg
    12721 tactgcgaga tcgccagccg tggatccagc gcgctggcag cttgcgccag gcacagggcg
    12781 accaccatcc atgccacgcg cagtaccgcc cgcatcgtgt tgccccgatg gactactgca
    12841 acccagtctc tgcgcaagca aatcgttgtg caatacgccc gcggatcggt cctgtggttc
    12901 gggcattgcc gttggattcg gctgaaacgc agctgttcgg cattgctaca ctgcggccat
    12961 gcaggaaccc gcgcgcccga aaaggaagac caaggccacc tcgctggaca tcgcccatcg
    13021 cgccggggtg tcgcaggcca cggtgtcgcg ggtgctgcgc ggcagcccgc tggtcaatgc
    13081 cgaaacccgc aagcgggtcg aggacgcggt gcgcgagctc aactacaagg tcgatcgcca
    13141 tgcctccagc ctgcgcaccc agcgcgccgg caccctggcg ctgctgctgt tcgaggaccc
    13201 gaccccggac gattcccaca tcaacccgtt cttcctgtcg atgctgggct cgatcacccg
    13261 cgcctgcgcg cgccacggcc acgacctgct ggtctcgttc cagcagctct ccgacgactg
    13321 gcatgccgac tacgaggaca gcatgaaggc cgacggcctg atcctgctcg gctacggcga
    13381 ctacctggcc tatgaaagca agctttcgca cctggtggcg cagggcaccc atttcgtgcg
    13441 ctggggcgcg gtgctgccgg ggcagccggg gctctcgatc ggctgcgaca acgccgaggg
    13501 cggcgcgctg gccgggcgcc acctgatcgc atccgggcgc aagaagatcg ccttcctcgg
    13561 cgatgcgtcc acccattacc cggagttctt cgcccgctat tgcgggtgcg acgccgccct
    13621 gcgcgaggca ggcctgcgca tgaatccggc gctgcaggtg gatgccacca gcaccgagca
    13681 gtccggccac gatgcggcga aggtgctgat cgcgcgcggg ctggcgttcg acgcggtgtt
    13741 cgccgccagc gacctgatcg cgatcggggc gatgcacgcg ttgaaggaac acgggctgcg
    13801 ggtgccggag gacgtcgcgg ttgtcggctt cgatggcacc ccgatggcgc gcttctccaa
    13861 tccgccgctg accacggtgg tgcaggacac ctcgaaggcc ggggaactgc tggtggacac
    13921 cctgctgcag ctggtgcgcg acgagccggc gcacagcatc acgcttgccc cgaccctgca
    13981 ggtccgccag tccagcggcg gctgagccag ccgccgcccc gctgcctcag cgcgggcgcc
    14041 tggcgtcgtc catcgccgcc gccagcgcgg cctccagcga aggctcgctg atttcggcat
    14101 ccagcaggaa cacctgcacg ccatgcgcgg ccacgtccgc ggccagcgca ccgtgggcgg
    14161 cgacctcgac cacgctgccg tccagcgcgg aacgccagcg cccggcctgc agcatcgacg
    14221 cgatctcgaa tcgctgcggc gcatcgccct tgttcagcag caccagcgcc agctgctgcc
    14281 ggccggcgtg ctggtaggcg cggtagaacg ccgcgcggtc gccctgcaac tcgatgttca
    14341 cctgcaggcc gcgttgcagc gcaggtgagg ccgcgcgcac cctggcgatg cgggtgagcg
    14401 cctgctggat cggatggccc ttcgctgctt cgatccgttc gctgccgaag tagttgcggt
    14461 tgccggcgtg ctcggcggtg ccgcgttcga agccgatctc cgagccgtag tagatgacgg
    14521 ggatgccgcg cgcggtgaac agccagttgt gcgcgtcgat gaagccgttg tcgctggcct
    14581 tcatccgcgg catgtcgtgg ttgtcgtaga acgtcatcag ttcgtacggg ttcgagtaag
    14641 gcccgccggt caggtgcagc ggcgcggcca gcgacgcgaa gtcgccgccg ggcttttcga
    14701 acaggttcgc cagcgcctgc ttcagcggga aatcgagcac gctgacgttc gcgttcccgg
    14761 gcagggtgtg ttccgcgatc ttgccggcct cgtagtcgaa ggcctcgccg aacatgaaga
    14821 agccgggatg cctgtcgcgg atcctgcgcg agaactcgtg ccagaaggaa tgcggcagcc
    14881 agccgatggt gtcgatccgg aacgcgtccg cgccttgctc gatccactgc tcgtaagcgc
    14941 ccagcaggta gggcagcacg cgcgggtcgt tctcgttgag gtccgacagc tgggcgatgc
    15001 ccggcttgct gttgtagaac gcgtgcagcg ggttctgcgg atcgagcttc tccggcgcga
    15061 gattcatgtg gtcggcgagc agggtgccgt cgcggtcgcg gatctcgccg aacttcggct
    15121 ggtcgaccgg catggtgtag gcgggcgaac catggttggc cacgatgtcc agcacggtct
    15181 tcagcccgtg cgcgcgcagg acgttggcca gtccgcggaa atcgaggccg ggcgagggca
    15241 ggtgctcgtc gagctggtag aagtcgacgc cccagtagcc gtggtagccg gtcttgccgc
    15301 catcggtcca gaagccttcc cacttgaccg gctcgccgcc ggtgaaggcc tcgtccgggt
    15361 tgtccacgat cggggtgatc cacaccgcgc cgaagcccat gtcgcggatg tacgcggcgt
    15421 ggtcggcgac gcccttgaag tcgccgccga ggtaaccgac gttggcgtgc cggccggcgg
    15481 gcgcgccggg taccgggcgg tcgaaggtga agaaggcctc gcccctggcc ttgccctggt
    15541 cgcggtggtc attggacgca tcgccgttga cgaagcggtc ggtgagcagg aagtacaccg
    15601 cgtcgctggc gaacggttcc agggtgccgt agtaggcggg tgcggcaccg gcggcgggcg
    15661 cgggtgcagg tgcgggggtg ctggcgcagc cggtggcggc gaaggccagg gcgaaggcag
    15721 ccgcaagcgg ggtggtggcg atgcgcatgt ggtcgtcccg gtcaggccgc atgcgcggcg
    15781 ggttcgggca cccgcagcac gcatagcccg gccacgacca tgctggcgcc gccaagcacc
    15841 aggatcttcg atgggtcgtc cgcgaacagg tgcttgagca ggtagcccag ggtggccacc
    15901 gcgaccagct gcgggatcac gatgaagaaa ttgaagatgc ccatgtacac gcccatcttg
    15961 cgcgccggca cgctgtcgga cagcagcgcg tacggcagcg acaggatcga ggcccaggcg
    16021 aagccgacgc cggccatcga cagcagcagc cagtcctcgt tcggcatgaa cggcagcgat
    16081 gccagcccga gcccgcccag cgaaacgttg accaggtggc tcatgcgcag ccccagtcgg
    16141 ctggccatgg ccgggatcgc gatcgccgcc agcgccgcga acccgttgta ggcggcgaac
    16201 agcacgccaa cccagttcgc gcctgtgttg tacgcggcgg aggcggtatc ggtgctgccg
    16261 tagtagaccg aggtgacggc ggcggtggtg tagatccaca tcgcgaacag cgcgaaccag
    16321 gagaagaact gcacgatggc gagccggcgc atcgcggtgg gcatcgcgta gatgtcggtc
    16381 atcacctgcg cgaagccgtt gtcggggccg accacgttca acgccagcag ggcgatgccg
    16441 taggcggcga gcagggcgcc gagcaggtac agctcgcgcg gcaggccctg caacgcgacc
    16501 agcgccagcc ccagcgcgcc gatgccgagc cacagcaggc cgaagcgggt ggcgcgcgcg
    16561 agctgcagcg gggcgcgatc gagcggtgcg gcttcgtcgt aggcgtgcag ggcgtcgggc
    16621 gggtattcgc gggtgcgcag caccgtccac gtgatcgcac cgagcagcac gccggcgccg
    16681 gcgtagaacg catagcgcac ggtatccggc acctgccccg catccgcggt gttggcgacg
    16741 ccccatttct ccagcatcca cggcagcatg ctggcgatca ccgagccgat gccgatgaag
    16801 aagctctgca tcgcgtagcc cttggcccgc tgcgagaccg gcagctggtc gccgacgaag
    16861 gcgcggaacg gctccatcga gatgttgatc gaggcgtcca gcacccacag caggcccgct
    16921 gcgatccaca gggtgggcga gtcgggcatg aacagcagcg cgatcgagga cagcaccgcg
    16981 ccgaccagga aatacgggcg gcggcggccg agcccggtcc aggtgcgatc ggacaggtag
    17041 ccgatgatcg gctgcacgac caggccggtg agcggcgccg cgatccacag gcccggcagc
    17101 gaatccatct ccgcgccaag ggtctggaag atgcggctga cgttggcgtt ctgcagcgcg
    17161 aaaccgaact ggatgccgag gaagccgaag cacatgttcc agatttgcca gaacgaaagt
    17221 tgcggcttgc gcgcgctcgg gttcgaagcc atcggagatc catcgctgca cgtgggagtt
    17281 gcgcgaagtg tgctgcagcg aaacgcctgc g
//
LOCUS       DAORUK010000028        17065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_3063_length_17065_cov_18.673604, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000028 DAORUK010000000
VERSION     DAORUK010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 17065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17065
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3063_length_17065_cov_18.673604"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PK818_03220"
     CDS             complement(<1..808)
                     /locus_tag="PK818_03220"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HQY81777.1"
                     /translation="MRTRPDAIHWCDGSDSENDALVARMQADGTLLKLNEQTHPDSWL
                     HRSDPDDVARVEHLTYVCTTHHEDAGPNNHWMAPDEAHAEMDALFDGCMQGRTMYVIP
                     YCMGPIDSPLSRCGVEITDSPYVVANMRIMTRMGAAALARIEREGAFVKGLHSIGELD
                     PNRRFIMHFPEELTIKSYGSGYGGNALLGKKCHALRIASHQARQEGWLAEHMLILGVE
                     NPQGETHYIAAAFPSACGKTNLAMLIPPAGYREAGWKVWTIGDDICWMRPG"
     gene            complement(967..1545)
                     /locus_tag="PK818_03225"
     CDS             complement(967..1545)
                     /locus_tag="PK818_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY81778.1"
                     /translation="MTTEALPRSGRLSADDWAREALEQIAEAGVGSVAVEPLARRLGV
                     TKGSFYWHFPSRDALLQAALERWEHDEQAAYGALESISDPTARLRSLFQLIAHHNKTH
                     VIYSELLKASDHAAVQAVLAHVLDRRIGFLFASFRQAGLSQKDALNRARMTYAVYVGF
                     LQLELPKFQARQPIEGLDGYIEHMMATLIPKS"
     gene            1730..4204
                     /locus_tag="PK818_03230"
     CDS             1730..4204
                     /locus_tag="PK818_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQY81779.1"
                     /translation="MSILLPFLILLAATAFAAYHRLRLAVWAAATATLLVACWLLGAD
                     KTATSVAAVLVALVALPLLLPFLRMPLITRPLLGFYTKILPPLTETERVALEAGTVGW
                     EGQLFSGKPDWNVLADQPRPTLRPDEQAFLDGPVEELCRMTNDWQITHVDADLSPELW
                     DYIKKNRFFGMIVPKEYGGLGFTALGNHKVIQKLASISAVVSSTVGVPNSLGPAELLM
                     HYGTDEQKQHYLPRLADGREVPCFALTGPFAGSDATSIPDYGIVCMGEWNGARVVGVR
                     LTFDKRYITLAPVATLVGLAFRMYDPDALLGDKRDIGISLALVPRDTAGLEIGRRAMP
                     LNSTFQNGPVRGKDVFIPLSQLIGGEAMAGKGWQMLVECLSIGRSITLPSTASGGAKI
                     GAVATGAYARIRKQFGLSIGRFEGVEEALARIGGNAYTISALAEASAAAVARGELPAV
                     PSTISKYHCTEMGRDVSRDVMDIIGGKGIILGPRNFAGRAYQAAPIAITVEGANIMTR
                     SLMIFGQGAILCHPWVLKEMKAATNPDAGARLRDFDHALFGHIGFAISNAVRSLWFGL
                     TGARIGAAPGDTYTRRYYRKLNRYSAALALMADTSMLMLGGKLKFKESLSGRLGDVLS
                     NLYIASALLKRYEDEGSPAADQPLLAWSIHNAVFKIENAFSGALRNFPIRPVGWLLWA
                     LVFPWGRRAQYPSDRLCHKVASLLMSPNEARDRLAVGVFTTPCENNPAGRIISYLPKV
                     VLAEPVERKLIKALKNSDIEALDFRAQLEEGVREGWITAEERVQLEELRVMTIDAISV
                     DDFDPAELRSACSRLEQDATEARAAA"
     gene            4305..4772
                     /locus_tag="PK818_03235"
     CDS             4305..4772
                     /locus_tag="PK818_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HQY81780.1"
                     /translation="MSVLATYRRLARWPAGRWLFSRLVCLKAPYFASIDPRMELLEPG
                     RGVATLRHRRAVSNHLGSVHAIALCNLAEFVGGLACDASIPPSMRWIPKGMTVAYLKK
                     AIGSMRATATPAFAPRESAQGYELPFEVVVANAQGEPVFTATIAMWVSPKTPR"
     gene            4905..6671
                     /locus_tag="PK818_03240"
     CDS             4905..6671
                     /locus_tag="PK818_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81781.1"
                     /translation="MQARNTGDATARIRLNIGLTAHRDVIDSEVPRLRREVRSFFQRL
                     QAEFPDLPLRLISALAEGGDQLVAEEALALGIELMVPLPMPQADYEQDFDRAGARERF
                     HALMARAQVRTLPLAPGNTAESLHQRGPARNRQYAQLGMFVSSHCQVLLALWDGKPSD
                     ATGGTAQVVDYHLRNAMPGFRAGDVAPNLLADDESDLVYHLHCPRRLPHPDAGEREAD
                     GLAGVPRWLTVEASVAGEAPMPAQYRHVFAQMQAFNHDAARHAAAIARDARSLLAADT
                     PAPAPPAVREVDALFGAADWLAVHFRQRVRRGMLLTHGLAAAMGLAFILYSDVDSDRR
                     YVAAFLAMFALGLLVKWIGERREWHRKYLDYRGLAEGLRVQVYWRLAGVETPPNSSLG
                     YDSFLQKQDVDLSWIRHAMRSTGLLRSEDFRTDADWLRWVIARWVGRRASATAVASGQ
                     LAYFSEGSRRREDAYRTTAVLGGVALAGGVLGALALLVAGGRLDPGLHGKLLLAMGLL
                     PLLAGIREAFSYKNADKELIKQFRFMSRLFDSCRARLDRAADDAESRQLLRALGCACL
                     EEHAEWILLHRERPLDVGGMSA"
     gene            6742..8838
                     /locus_tag="PK818_03245"
     CDS             6742..8838
                     /locus_tag="PK818_03245"
                     /inference="COORDINATES: protein motif:HMM:NF020509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HQY81782.1"
                     /translation="MRYRAFISYSHADARQATWLHRQLEGYRLPSRLRGGSGEHGPLP
                     ERLVPIFRDRDDLSSAGQLGPQIEAALADSEALVVVCSPSAARSPFVDSEILAYKRLG
                     RGDRIYAFIIDGEPNAGDQRECFPAALRFELEPDGSIGSTPANPIAADARAGKDGKSL
                     ARLKLLAGLFGLPLDTLRQREAQRRHRRMAAVTALAVLVMLVTSVLAVQAVVAQKAAE
                     RRQKQAEALVDFMLGDLNDKLSEVGRLDILSAVNDKAMAYFKSLPNTDVTAAVLQQRA
                     KALVKIGVVRSDQGELSKAMESFEAASKLSGALARAAPANVATQLAHAEVLAYVGLSH
                     WYQGDLARAEQGFDAAYDVLAGARRLAPDDPQLLFQLSTVENNNGHVLEARGQIEQAT
                     GHYRRMLEAAQRLTTLAPDEDDWQNQLALAHNNLAKMALLGGNLSMAIEGYRRQVAIQ
                     RALAARDPRNNAQRRGLLIARFTLGRTLALMGQAEEGRRLMQQALDEARALHAMEPSS
                     TDFQSDVGLYALQLARLERQLGNSESALQLAQESAGLFDALVRASKDQPTWQSRQAEA
                     ITERAHLAIAAGTREGDLDTQLRKALQTLEAQLAQSPQDRSSVLATVDARLRLAAISS
                     DPERTRLATTSLATIEAQASARNDPRLRVLEADALRQLGRRADASAVDTALRASGYRA
                     PAVARVARAAAASLNP"
     gene            8911..9306
                     /locus_tag="PK818_03250"
     CDS             8911..9306
                     /locus_tag="PK818_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81783.1"
                     /translation="MTNNINVTLDTSVTPHELKVHDHGHVRIDKKPGAQLISWNLTGN
                     LAQGSFLPICGNSPGFEWVSDPPPPGGIFGEPQVFAKSLTLVDIHPDGGSDGTWIYRL
                     RVCLDGTVYSTSSSAGVSDVRGNPIIIND"
     gene            9403..9567
                     /locus_tag="PK818_03255"
     CDS             9403..9567
                     /locus_tag="PK818_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81784.1"
                     /translation="MGNSKKPHQDDQDNDGIADTGQSQSQAHAGQDQDGDGVPDGDTS
                     VQSNPIIINH"
     gene            complement(9605..10969)
                     /locus_tag="PK818_03260"
     CDS             complement(9605..10969)
                     /locus_tag="PK818_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY81785.1"
                     /translation="MTDTTASTARIGGRMGNFRAALAESPPLLWSFLYFFCLLSGYYV
                     LRPVREAMGASSDVAAVFPPAMIEFFAVRGIELKELVLQVLFTCTFLIMLVLQPVYGW
                     LVSRFPRRVFLPVVYGFFIATLLVFYAMFHGGVPGRGMAFFLWITVFNLFAVAVFWSF
                     MADVYDDAEARRYYGYIGAAGTLGAIFGPMLTRTLVERIGIANLMLVSAGFLLLCIVC
                     LLRLRRHAVQRELQRNLASGELPIGGQVLAGLKLVVREPLLRWMAVLTLFGVGVGTLL
                     YNEQAAIVRQFHPDPKLATAYYANIDLAVNALTLFVQLFVTRALLSRFGIGPALLVPG
                     GAIILGYAALAASPLPLLVATVQVVTRASEFSLAKPARETIYTRVGREWRYKAGAAID
                     TVVYRGGDLTFVWLHKLLSAFGSQVVFGAGLLVAMGMTLGAWKVVGEARKLPAARPPA
                     DPPA"
     gene            11095..12690
                     /locus_tag="PK818_03265"
     CDS             11095..12690
                     /locus_tag="PK818_03265"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HQY81786.1"
                     /translation="MIHDGRFDYIIIGAGSAGCVLANRLSEDPDVKVLLLEAGPRDWH
                     PFIHMPAGLAKLVNRKGVNWNYDTAPEPHLHKRVLWWPRGKVLGGSSSINAMCYIRGV
                     ARDYDEWGQVAQGWDWNGVLPYFRRSEGNSRGGDALHGADGPLSVSDLRHSNPLSQVF
                     LDAAGQVGIPANGDFNGPSQEGVGLYQVTQRDGARCSAAVAYLNPAKSRRNLTVHTGA
                     LVNRITFDGARRANGVTYSMRGKAFHQPAAREVILCGGTINSPQALMLSGIGPADALR
                     SLGIDVVHDSPGVGGNLQDHLDICTLQHSTRRITYDRINEPRMAFDYYLRGHRGAGSS
                     NIAEAGGFWRSPLATDERCDVQFHFVPAMLDDHGRRRLPGDGYTLHACFLRPRSRGRL
                     TLASNRASDQPRIEANYLGDAEGFDLKVMLECAKVSRSIFAQRAFDPYRGAPIFPARN
                     DLSDAELLEFIRAKAESVYHPVGTCRMGDDDDAVVDASLRVRGVEGLRVVDASVMPNL
                     PGGNTNAPTIMIAERAADLIRAG"
     gene            complement(12751..14637)
                     /locus_tag="PK818_03270"
     CDS             complement(12751..14637)
                     /locus_tag="PK818_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQY81787.1"
                     /translation="MNFFEHQAAARRMSSRLLLLFALAVAGIVAAVDAVAWLLTGSVQ
                     VVAFTSVATLAVIGFGSMYRIASLRGGGDAVALQMGGVPVPEDATDLNLRRLRNVVEE
                     IAIASGVPVPRVYVLEHEAAINAFAAGYSSSDAVVAVTRGALDRLNRDELQGVVAHEF
                     SHILNGDMRLNIRLVGVLFGILMIGLIGRKILAPGRFGGRGKGAGGVLGVALVAIVVG
                     YIGLFFGRMIKAGVSRSREVLADASAVQFTRQAQGLAGALKKIGGLHEGARLNDRGDA
                     EEVSHMLFGDGVGFSGLFATHPPLLKRIQALEPGFDGGALQALQAKWAASPPSGLDED
                     RHMGLGGDSGTRLPLAAQQLEVTPRTVAAQVATPAADDVRRADGIVAAIPEALRDLAA
                     GRETVMPLLLGLLLADDDALAARQQAEIGARLGESHADHALRIHQQLVADLHPMLRLP
                     LAALAFPVLRLRPRPELDAFLATVAAVVAADGEVSLFEYCLGRLLAVQVRESLDPSRY
                     ARFGRRKPGNVRQEFATLLAVVAQAGHADAASARRAYLAGMQRVLPRDHVPYAPPANG
                     VQALDAVWEPLDALDPLAKQVMVEAVTDAVSHDGRVSVAEAELLRTICGVLHCPLPPM
                     LESS"
     gene            complement(14692..15288)
                     /locus_tag="PK818_03275"
     CDS             complement(14692..15288)
                     /locus_tag="PK818_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQY81788.1"
                     /translation="MLTLLILLVIVAAVAFWGVGIYNGLVTARNAFKNAFAQIDVQLQ
                     RRFDLIPNLVEVARKYMSHERETLEAVIAARAAAQSGLSAAKASPGDPEAMAQLVAAQ
                     GQLNAGLGRLLAVAEAYPDLKANQNMMQLTEELTSTENKVAFARQAFNDAVMAYNNRR
                     EMFPNSIFAGMFGFTAATLLEIPAERKADVRAAPKVQF"
     gene            15371..16633
                     /locus_tag="PK818_03280"
                     /pseudo
     CDS             15371..16633
                     /locus_tag="PK818_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437736.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
     gene            complement(16815..>17065)
                     /locus_tag="PK818_03285"
     CDS             complement(16815..>17065)
                     /locus_tag="PK818_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81789.1"
                     /translation="KAAAKKPAAKKAVKKAAAKKPAAKKAVKKAAAKKPAAKKAVKKA
                     AAKKPAAKKAAAKKPAAKKKAAKKAKPVAMPAMPAPMM"
BASE COUNT         2674 a         6044 c         5851 g         2496 t
ORIGIN      
        1 cgccggggcg catccagcag atgtcgtcgc cgatcgtcca caccttccag ccggcctcgc
       61 ggtagcccgc cggcgggatc agcatggcca ggttggtctt gccgcaggcg ctggggaacg
      121 ccgcggcgat gtagtgggtc tcgccctgcg ggttctccac gcccaggatc agcatgtgct
      181 ccgccagcca gccttcctgg cgcgcctggt ggctggcgat gcgcagggca tggcacttct
      241 tgccgagcag ggcgttgccg ccgtagccgg agccgtacga cttgatggtc agttcttcgg
      301 ggaaatgcat gatgaagcgg cggttggggt ccagctcgcc gatcgaatgc aggcccttga
      361 cgaatgcgcc ttcgcgctcg atccgcgcca gcgcggccgc gcccatgcgg gtcatgatcc
      421 gcatgttggc gaccacgtag ggcgagtcgg tgatctccac cccgcagcgc gacagcggcg
      481 agtcgatcgg ccccatgcag tacgggatga catacatcgt gcggccctgc atgcagccgt
      541 cgaacagggc gtccatctcg gcgtgggcct cgtccggcgc catccagtgg ttgttcgggc
      601 cggcgtcctc gtggtgggtg gtgcacacgt aggtcaggtg ctcgacgcgg gccacgtcgt
      661 ccggatccga gcggtgcagc cagctgtccg gatgggtctg ttcgttgagc ttgagcaggg
      721 tgccgtcggc ctgcatgcgc gcgaccaggg cgtcgttctc gctgtcgctg ccgtcgcacc
      781 agtggatcgc gtccgggcgg gtgcgcatgg cgacgctggc cacccagtcg ttcagcgcag
      841 cgagggcgct gccggtggaa gtggcgtcgg agctgtgttt ggagagtgcg ttcatggagt
      901 ggcctcggcc caggcgggca ccggtgggac gcgggcgacg ccgcgtccgg ggtcaggatt
      961 cggggttcag gacttcggga tcagggtcgc catcatgtgt tcgatgtagc cgtcgagccc
     1021 ctcgatcggc tggcgcgcct ggaacttcgg caattcgagc tgcaggaagc cgacgtagac
     1081 ggcataggtc atgcgcgccc ggttcagcgc gtccttctgg ctcaggccgg cctggcggaa
     1141 ggatgcgaac aggaagccga tgcggcggtc cagcacgtgc gccagcaccg cctgcacggc
     1201 ggcatggtcg ctcgccttca gcagctcgga atagatgacg tgggtcttgt tgtggtgcgc
     1261 gatcagctgg aacagcgaac gcagccgcgc agtcgggtcc gagatcgact ccagcgcccc
     1321 gtaggcggcc tgttcgtcgt gctcccagcg ctccagtgcc gcctgcagga gggcgtcgcg
     1381 tgacgggaaa tgccagtaga agctgccctt ggtgacccca agccggcgtg ccagcggttc
     1441 caccgccacc gaaccgacgc cggcctcggc gatctgctcg agtgcctcgc gggcccagtc
     1501 gtcggcgctc aggcgcccgc tgcgtggtaa ggcttcggtg gtcatcctgg tctttgtgcg
     1561 tccatacgcc ggggcttgga ggggtatgct cgggaagtat gctgagtttt tgctcggatt
     1621 cgccaattga cagcggcgac cgacacgcca tacttgaccg tatggtacga aacgctgcaa
     1681 ccgcatgcaa cgttgccatg cccagatacc cggcccccgg agtccccaca tgagcatcct
     1741 gcttcccttc ctgatcctgc tggccgcgac cgcgttcgcg gcctaccacc gcctgcgcct
     1801 cgcagtctgg gccgcggcga cggccaccct gctggtcgcc tgctggctgt tgggcgccga
     1861 caagaccgcc accagcgtgg ctgccgtgct ggtggcgctg gtcgcgctgc cgctgctgct
     1921 gccgttcctg cggatgccgc tgatcacccg gccgctgctc ggcttctaca ccaagatcct
     1981 gccgccgctg acggaaaccg agcgggtggc gctggaagcc ggcaccgtgg gctgggaagg
     2041 ccagctgttc tccggcaagc cggactggaa cgtgctggcc gaccagccca ggcccaccct
     2101 gcgcccagac gagcaggcgt tcctggacgg cccggtcgaa gaactgtgcc ggatgaccaa
     2161 cgactggcag atcacccacg tcgacgccga cctctcgccg gagctgtggg actacatcaa
     2221 gaagaaccgc ttcttcggga tgatcgtgcc gaaggaatac ggcggcctgg ggttcaccgc
     2281 gctgggcaac cacaaggtga tccagaagct ggcatcgatc agcgcggtgg tcagttccac
     2341 cgtcggcgtg ccgaactcgc tgggtccggc cgagctgctg atgcactacg gcaccgacga
     2401 acagaagcaa cactacctgc cgcgcctggc cgatggtcgc gaggtgccct gcttcgccct
     2461 gaccggcccg ttcgccgggt cggatgcgac ctcgatcccc gactacggca tcgtctgcat
     2521 gggcgaatgg aacggcgcgc gggtggtcgg cgtgcgcctg accttcgaca agcgctacat
     2581 caccctggca ccggtcgcca ccctggtcgg cctggccttc cgcatgtacg acccggatgc
     2641 cctgctgggc gacaagcgcg acatcggcat ttcgctggcg ctggtcccgc gcgacaccgc
     2701 cggcctggag atcggccgcc gcgcgatgcc gctcaacagc acgttccaga acggcccggt
     2761 gcgcggcaag gacgtgttca tcccgctcag ccagctgatc ggcggcgagg cgatggccgg
     2821 caagggctgg cagatgctgg tcgagtgcct gtcgatcggg cgctcgatca ccctgccctc
     2881 caccgccagc ggcggcgcca agatcggcgc ggtggcgact ggcgcgtacg cacgcatccg
     2941 caagcagttc gggctgtcga tcggccgctt cgagggcgtc gaggaagcgc tggcgcggat
     3001 cggcggcaac gcctacacca tcagcgcgct ggccgaggcc tcggcggcgg cggtggcgcg
     3061 tggcgaattg ccggcggtgc cgtccaccat ctccaagtac cactgcaccg agatgggccg
     3121 cgacgtttcc cgcgacgtga tggacatcat cggcggcaag ggcatcatcc tcggcccgcg
     3181 caacttcgcc ggccgcgcct accaggcggc gccgatcgcg atcacggtgg aaggcgcgaa
     3241 catcatgacg cgctcgctga tgatcttcgg ccagggcgcg atcctgtgcc acccgtgggt
     3301 gctgaaggag atgaaggcgg cgaccaaccc ggacgccggc gcgcgcctgc gcgacttcga
     3361 ccacgcgctg ttcggccaca tcggcttcgc gatctcgaac gcggtgcgct cgctgtggtt
     3421 cggcctcacc ggcgcgcgca tcggcgcggc gccgggcgac acctacaccc gccgctacta
     3481 ccgcaagctc aaccgctact cggcggcgct ggcgctgatg gcggacacct cgatgctgat
     3541 gctgggcggc aagctgaagt tcaaggaatc gctgtcgggc cgcctcggcg acgtgctcag
     3601 caacctgtac atcgccagcg ccctgctcaa gcgctacgag gacgaaggca gcccggccgc
     3661 cgaccagccg ctgctggcgt ggtcgatcca caacgcggtg ttcaagatcg agaacgcgtt
     3721 ctccggcgcg ctgcgcaact tcccgatccg cccggtcggc tggctgctgt gggcgctggt
     3781 gttcccgtgg ggccgccgcg cgcagtaccc gagcgaccgc ctgtgccaca aggtcgcctc
     3841 gctgctgatg tcgccgaacg aggcgcgcga ccggctggcg gtgggcgtgt tcaccacccc
     3901 ctgcgagaac aatccggccg gccgcatcat cagctacctg ccgaaggtgg tgctggccga
     3961 accggtcgag cgcaagctga tcaaggccct gaagaacagc gacatcgagg cgctcgattt
     4021 ccgggcgcag ctggaagagg gcgtgcgcga aggctggatc accgccgagg agcgggtgca
     4081 gctggaggaa ctgcgggtga tgacgatcga cgcgatcagc gtcgacgact tcgacccggc
     4141 ggaactgcgc tctgcctgct cgcggctgga gcaggacgcc acggaagcgc gcgcggctgc
     4201 atgagccccc gccgcgatcc cggcaacccg ccgggatcgc ggcgtcggca ccatcccacc
     4261 acttgcctgt gacgcccggc ccggccgggc aagcgagccc cgagatgtcc gtgcttgcca
     4321 cctaccgccg cctggcccgc tggcccgcgg gccgctggct gttttcccgg ctggtgtgcc
     4381 tgaaggcgcc ctacttcgcc agcatcgacc cgcgcatgga actgctggag ccggggcgtg
     4441 gcgtcgccac cctccgccat cggcgcgcgg tcagcaacca cctcggcagc gtgcacgcga
     4501 tcgcgctgtg caacctggcc gagttcgtcg gcggcctcgc ctgcgatgcc agcatcccgc
     4561 cgtcgatgcg ctggatcccc aagggcatga ccgtggccta cctgaagaag gcgatcggca
     4621 gcatgcgggc gaccgcaacg cccgcgttcg cgccgcggga atccgcgcaa ggctacgaac
     4681 tgccgttcga ggtggtggtc gcgaacgcgc agggcgagcc ggtgttcacc gccaccatcg
     4741 cgatgtgggt gtcgccgaaa accccgcgct gacccggcgc caaggccact ggccacgcga
     4801 ccgcttgcga tgccatcctg cgtttgcgcg gggatgaggt tcgcctgcgc gcggacgtgg
     4861 tggcgccgga cccggcgtgc gccggcaggc aaggggacgc ttggatgcag gcacgcaaca
     4921 ccggcgacgc caccgccagg atccgcctca acatcgggct gaccgcgcat cgcgacgtca
     4981 tcgacagcga ggtcccgcgc ctgcgccgcg aggtgcgcag tttcttccag cgcctgcagg
     5041 ccgaattccc ggacctgccg ctgcggctga tttccgcgct ggccgagggc ggcgaccagt
     5101 tggtggccga ggaggcgctg gcgctcggga tcgagctgat ggtgccgctg ccgatgccgc
     5161 aggcggacta cgaacaggac ttcgatcgcg ccggtgcgcg cgagcgcttc cacgcgctga
     5221 tggcccgcgc gcaggtgcgc acgctgccgc tggcgcccgg caacaccgcg gaatccctcc
     5281 accagcgcgg accggcgcgc aaccgccagt acgcgcagct ggggatgttc gtgtcctcgc
     5341 actgccaggt actgctggcg ctgtgggatg gcaagcccag cgacgccacc ggcggcaccg
     5401 cgcaggtggt ggactaccac ctgcgcaatg cgatgcccgg gttccgcgcc ggcgacgtcg
     5461 ccccgaacct gctggccgac gacgagagcg acctggtcta ccacctgcac tgcccgcgcc
     5521 ggctgccgca tcccgacgcc ggcgagcgcg aggcggacgg cctggccggg gtcccgcgct
     5581 ggctcaccgt ggaagccagc gtggccggcg aggcgccgat gccggcgcaa taccgccacg
     5641 tgttcgcgca gatgcaggcg ttcaaccacg atgccgcgcg gcatgcggcg gcgatcgcgc
     5701 gcgacgcgcg ctcgctgctg gcggccgaca ccccggcgcc ggcgccaccg gcggtgcgcg
     5761 aggtggatgc gctgttcggc gccgccgact ggctggccgt gcatttccgc cagcgcgtgc
     5821 gccgcgggat gctgctgacc catgggctgg cggcagcgat gggcctggcc ttcatcctgt
     5881 attccgacgt cgacagcgac cgccgctacg tcgccgcgtt cctggcgatg ttcgcgctgg
     5941 gcctgctcgt gaagtggatc ggcgaacgcc gcgaatggca ccgcaagtac ctcgactacc
     6001 gcggcctcgc cgaagggctg cgggtgcagg tgtactggcg gctggccggg gtggagacgc
     6061 cgccgaacag cagcctgggc tacgacagct tcctgcagaa gcaggacgtc gacctgagct
     6121 ggatccgcca cgccatgcgc agcaccgggc tgctgcgcag cgaggacttc cgcaccgacg
     6181 ccgactggct gcgatgggtg atcgcgcgct gggtggggcg gcgcgccagc gcgaccgccg
     6241 tcgccagcgg gcagctggcc tatttcagtg aaggcagccg caggcgcgag gatgcctatc
     6301 gcaccaccgc cgtgcttggc ggcgtcgccc tggccggcgg cgtgctcggc gcgctggcgc
     6361 tgctggtcgc cggcggacgc ctggacccgg gcctgcacgg caagctgctg ctggcgatgg
     6421 gcctgctgcc gctgctggcg ggtatccgcg aagcgttttc gtacaagaac gcggacaagg
     6481 agctgatcaa gcagttccgc ttcatgagcc gcctgttcga cagttgccgc gcgcgcctgg
     6541 accgcgcggc cgacgacgcc gagtcgcgcc agttgctgcg tgcgctgggc tgcgcctgcc
     6601 tggaggaaca cgcggagtgg atcctgctgc atcgcgagcg gccgctggac gtgggcggga
     6661 tgtccgcatg agccgcggct tcgcgttgcc cactgggttg gcccgcgtgc ctggccacgg
     6721 ctgaaacccg ggaggtgtgg cttgcgttat cgcgccttca tcagctacag ccacgccgac
     6781 gcgcgccagg ccacctggct gcaccggcag ctggagggct accgcttgcc gtcgcggctg
     6841 cgcggcggca gcggcgaaca cggcccgctg ccggagcggc tggtgccgat cttccgcgac
     6901 cgcgacgacc tctccagcgc cggccagctg ggcccgcaga tcgaggcagc gctggccgac
     6961 tcggaggcgc tggtggtggt gtgttcgccg agcgcggcgc gctcgccgtt cgtggattcg
     7021 gaaatcctcg cctacaagcg gcttggccgc ggcgaccgca tctatgcctt catcatcgac
     7081 ggcgaaccca atgccggcga ccagcgcgag tgcttcccgg cggcgttgcg tttcgagctt
     7141 gagcccgacg gcagcatcgg cagcaccccg gccaacccga tcgccgccga cgcccgcgcg
     7201 ggcaaggacg gcaagtcgct ggcgcggctg aagctgctgg ccggcctgtt cggcctgccg
     7261 ctggacaccc tgcgccagcg cgaagcgcaa cgccggcacc ggcgcatggc cgcggtgacc
     7321 gcgctggcag tgctggtgat gctggtcacc agcgtgctgg cggtgcaggc ggtggtggcg
     7381 caaaaggccg ccgagcgccg gcagaagcag gccgaggcgc tggtggactt catgctgggc
     7441 gacctcaacg acaagctctc ggaagtgggc cggctggaca tcctgtccgc ggtcaacgac
     7501 aaggcgatgg cctatttcaa gtcgttgccg aacaccgacg tcaccgcggc ggtgctgcag
     7561 caacgcgcca aggcgctggt caagatcggt gtagtccgct ccgatcaggg cgagttgtcg
     7621 aaggcgatgg agagtttcga ggcggcgtcg aagctgtcgg gcgcgttggc acgggctgcc
     7681 ccggccaacg tggcgacgca actggcacat gccgaagtgc tggcctacgt cggactcagc
     7741 cactggtacc agggcgacct tgcgcgcgcg gagcagggct tcgatgcggc gtatgacgtc
     7801 ctcgccggtg cgcgccgcct ggcgcccgac gatccgcagc tcctcttcca gttgtcgacc
     7861 gtcgagaaca acaacggcca cgtgctcgag gcccgcgggc agatcgaaca ggcgaccggc
     7921 cactaccgac gcatgctgga ggcggcacag cggctgacca cgcttgcgcc ggacgaggac
     7981 gactggcaga accagctggc gctggcgcac aacaacctgg cgaaaatggc gttgctgggc
     8041 ggaaacctgt cgatggcgat cgaggggtat cgccgccagg tcgcgatcca gcgcgcgctg
     8101 gccgcgcgcg atcccaggaa caatgcgcaa cgccgcggcc tgctgatcgc ccgcttcacc
     8161 ctgggccgca cgcttgcact gatggggcag gcggaagaag ggcgccgact gatgcagcag
     8221 gcgctggatg aagcacgcgc actccacgcg atggagccca gcagcacgga cttccagagc
     8281 gacgttggcc tgtacgcgct gcaactcgcc cggctggaac gccagctcgg caattccgaa
     8341 tcggcgttgc aactggcaca ggagtccgcc gggctgttcg atgcattggt cagggccagc
     8401 aaggaccagc ccacctggca gagccggcaa gcggaggcca tcaccgagcg tgcgcacctg
     8461 gcgatcgccg ccggcacgcg cgaaggcgat ctggacaccc agcttcgaaa ggccctgcaa
     8521 accctggaag cgcaactggc ccagagtccg caggaccgca gcagcgtgct tgccacggtc
     8581 gacgcccgcc tgcgcctggc cgcgatttcc agtgatccgg aacggactcg gctcgccacc
     8641 acgtcgcttg ccaccatcga agcgcaggca tccgcgcgca acgacccgcg cctgcgcgtg
     8701 cttgaagccg atgcgttgcg ccagcttggg cgcagggccg acgccagcgc cgtcgacacg
     8761 gcactgcggg cctctggcta tcgggcaccc gccgttgcca gggtggcgcg cgccgccgcg
     8821 gcttccctca acccctgaac cagtccgcgt ccccgccagc cgcggagcgg cggcgccact
     8881 gcacggatgc gccaccgcac ggagctcctg atgacaaaca acatcaacgt gactctggat
     8941 acgtcggtta ccccccacga gctgaaggtc cacgaccacg ggcatgtccg catcgacaag
     9001 aagcccggcg cccagctcat cagctggaac ctgaccggaa atcttgcgca aggcagcttc
     9061 ctcccgatct gcggcaattc cccgggtttc gagtgggtga gcgacccgcc accgccagga
     9121 ggcatcttcg gcgagccgca ggtcttcgcg aagtcgctca ccctcgtcga tatccatccc
     9181 gacggtggca gcgacggtac ctggatctac cgcctgaggg tgtgcctgga tggaaccgtg
     9241 tactcgacca gctcgagcgc tggcgtctcc gacgtccgcg gcaatccgat catcatcaac
     9301 gactgagctc gccaaccgac gaacggcacg cttttccacc agccccggcg tagcctgccg
     9361 aggctggcca ttccgccaac gcacttccgc aggagcatcc caatgggcaa ttcgaagaag
     9421 ccgcaccagg acgaccagga caacgacggc atcgccgaca ccggccaatc gcagtcgcaa
     9481 gcgcatgccg gccaggacca ggatggcgac ggcgtccccg acggcgacac cagcgtccag
     9541 tccaacccga tcatcatcaa ccactgaccg cttcgcgccc gccgcgcgtg cggcgggcgc
     9601 gggctcaggc tggcgggtcg gccggcggcc gcgccgcggg cagtttcctg gcctcgccca
     9661 ccaccttcca cgcgcccagg gtcatgccca tcgcgaccag caggcccgcg ccgaacacca
     9721 cctgcgagcc gaacgcggac agcagcttgt gcagccacac gaaggtgagg tcgccaccgc
     9781 ggtagaccac ggtgtcgatc gcggcgccgg ccttgtagcg ccattcgcgg ccgacccggg
     9841 tgtagatcgt ctcgcgcgcg ggcttcgcca gcgagaattc gctggcgcgg gtcaccacct
     9901 gcaccgtcgc caccagcagc ggcaacggtg atgcggccag cgcggcatag cccaggatga
     9961 tcgcgccgcc cgggaccagc agcgccgggc cgatgccgaa gcgcgacagc aacgcgcggg
    10021 tgacgaacag ctgcacgaac agggtcagcg cattcaccgc caggtcgatg ttcgcgtagt
    10081 acgccgttgc cagcttcggg tcgggatgga actgccgcac gatcgcggcc tgttcgttgt
    10141 acagcagggt gccgacgccg acgccgaaca gggtcagcac cgccatccag cgcagcagcg
    10201 gttcgcgcac caccagcttc agcccggcca gcacctgccc gccgatcggc agctcgccgc
    10261 tggcgaggtt gcgctgcagc tcgcgctgca ccgcatgccg gcgcaggcgc agcaggcaga
    10321 cgatgcacag cagcaggaac ccggccgaga ccagcatcag gttggcgatg ccgatgcgtt
    10381 cgaccagcgt gcgcgtcagc atcggcccga agatcgcgcc cagggtgccg gccgcgccga
    10441 tgtaaccgta gtagcggcgt gcctcggcgt cgtcgtagac gtcggccatg aagctccaga
    10501 acaccgccac cgcgaacagg ttgaacaccg tgatccacag gaagaacgcc attccccggc
    10561 cgggcacgcc gccgtggaac atggcgtaga acaccagcag ggtggcgatg aagaagccgt
    10621 acaccaccgg caggaacacc cggcgcggga aacggctgac cagccagcca tacaccggct
    10681 gcaacaccag catgatcagg aacgtgcagg tgaacagcac ctgcagcacc agttccttca
    10741 gttcgatgcc gcgcaccgcg aagaattcga tcatcgccgg cgggaacacc gcggcgacgt
    10801 cgctggaggc gcccatcgcc tcgcgcaccg ggcgcagcac gtagtagccg ctgagcaggc
    10861 agaagaagta caggaacgac cacagcagcg gcggcgattc ggccagcgcg gcgcggaaat
    10921 tgcccatcct gccgccgatg cgcgcggtgc tggcggtggt gtccgtcatc tgcggggctg
    10981 ggacctgcgc gcggcgatgc gcaacggtaa ccgcagtcga tagcggacac cagcctgagc
    11041 atttgctcta gcgccggggc atagcgtcgg ggggtcatcg cagcgggacg gcacgtgatc
    11101 cacgacggca ggttcgacta catcatcatc ggcgccggct cggccggctg cgtgctcgcc
    11161 aaccgcctga gcgaagaccc ggacgtcaag gtgctgctgc tggaagcggg cccgcgcgac
    11221 tggcacccct tcatccacat gcccgccggg ctggccaagc tggtcaaccg caagggcgtg
    11281 aactggaact acgacaccgc accggagcca cacctgcaca agcgcgtgct gtggtggcca
    11341 cgcggcaagg tgctgggtgg ctccagctcg atcaacgcga tgtgctacat ccgcggcgtg
    11401 gcgcgcgact acgacgaatg gggccaggtt gcgcaaggct gggactggaa cggcgtgctg
    11461 ccgtatttcc ggcgttccga gggcaacagc cgcggtggcg acgcgctgca cggcgccgac
    11521 ggtccgctca gcgtcagcga cctgcgccac agcaacccgc tgtcgcaggt cttcctcgac
    11581 gccgccggcc aggtgggcat ccccgccaat ggcgatttca acgggcccag ccaggaaggc
    11641 gtcggcctgt accaggtcac ccagcgcgat ggcgcgcgct gctctgccgc ggtcgcctac
    11701 ctcaacccgg cgaagtcacg gcgcaacctc accgtgcata ccggcgcgct ggtcaaccgc
    11761 atcaccttcg atggcgcgcg ccgcgccaac ggcgtgacct actcgatgcg gggcaaggcc
    11821 ttccatcagc ccgctgcgcg cgaagtcatc ctctgcggcg gcaccatcaa ttccccgcag
    11881 gcgctgatgc tgtccgggat cggcccggcc gacgccttgc gcagcctcgg catcgacgtg
    11941 gtccacgatt cccccggcgt cggcggcaac ctgcaggacc acctggatat ctgcaccctg
    12001 cagcattcga cccggcgcat cacctacgac cgcatcaacg aaccgaggat ggcgttcgac
    12061 tattacctgc gcggccatcg cggcgccggc agcagcaaca tcgccgaggc cggcggcttc
    12121 tggcgctcgc cgctggcgac cgacgagcga tgcgacgtgc agttccactt cgtgccggcg
    12181 atgctggacg accacggccg ccgccgcctt ccgggcgacg gctacaccct gcacgcctgc
    12241 ttcctgcgcc cgcgcagccg cggccggctg accctggcca gcaaccgtgc cagcgaccag
    12301 ccccggatcg aagcgaacta cctcggcgat gccgagggct tcgacctgaa ggtgatgctc
    12361 gagtgcgcga aggtctcccg ttcgatcttc gcccagcgcg cgttcgatcc ctatcgcggg
    12421 gcgcccatct tcccggcccg caacgacctg tccgatgccg aactgctgga gttcatccgg
    12481 gccaaggcgg agtcggtgta ccacccggtc gggacctgcc ggatgggcga cgacgacgac
    12541 gccgtggtcg atgcctcgct gcgcgtgcgc ggcgtcgaag gcctgcgcgt ggtggatgcc
    12601 tcggtgatgc cgaacctgcc gggcggcaac accaatgcgc cgacgatcat gatcgcggag
    12661 cgcgcggcgg acctgatccg ggcgggttga gcgcccgggc atcgcgccgg cggctgcgcc
    12721 gggatccgcg gacccgggtg ggcgcctgcc tcagctggat tcgagcatcg gcggcagcgg
    12781 gcagtgcagc accccgcaga tggtgcgcag cagttccgcc tcggccacgc tgacccggcc
    12841 gtcgtggctg accgcatcgg tcaccgcctc caccatcacc tgcttggcca gcggatccag
    12901 cgcatccagc ggctcccaca ccgcatccag cgcctgcacg ccgttcgccg gcggcgcata
    12961 cggcacatgg tcgcgcggca gcacccgctg catgccggcg aggtaggcgc ggcgggcgga
    13021 ggcggcatcg gcatgcccgg cctgcgccac caccgccagc agggtcgcga attcctggcg
    13081 cacgttgccg ggcttgcgcc ggccgaagcg cgcgtagcgg gaaggatcca gcgactcgcg
    13141 cacctgcacc gccagcagcc ggcccaggca gtactcgaac agcgagacct cgccgtcggc
    13201 ggcgaccacc gccgcaaccg tggccaggaa cgcatccagc tccgggcgcg ggcgcagccg
    13261 cagcaccggg aacgccagcg ccgccaacgg cagccgcagc atcgggtgca ggtcggcgac
    13321 cagctgctgg tggatgcgca gcgcgtggtc ggcgtgggat tcgccaaggc gcgcgccgat
    13381 ttccgcctgc tggcgcgcgg caagggcgtc gtcgtcggcc agcaacaggc cgagcagcag
    13441 cggcatcacc gtctcgcgcc ccgcggcgag gtcgcgcagc gcctccggga tcgcggcgac
    13501 gatgccgtcg gcgcggcgga cgtcgtcggc cgcgggggtc gccacctgcg ccgccaccgt
    13561 gcgcggggtc acctcgagct gctgcgccgc caacggcagg cgcgtgccgc tgtcgccacc
    13621 caggcccatg tgccggtctt cgtcgagccc gctcggcggc gaagccgccc acttcgcctg
    13681 cagggcctgc agggcaccgc catcgaaccc gggctccagc gcctggatcc gcttcagcaa
    13741 cggcgggtgg gtggcgaaca ggccggagaa cccgacccca tcgccgaaca gcatgtggct
    13801 gacttcctcg gcatcgccgc ggtcgttgag cctggcgcct tcgtgcaggc cgccgatctt
    13861 cttcagcgcg ccggccaggc cctgcgcctg gcgggtgaac tggaccgcgc tggcgtcggc
    13921 caggacctcg cgcgagcggc tcacgccggc cttgatcatg cgtccgaaga acagcccgat
    13981 gtagccgacc acgatcgcca ccagtgccac cccgagcacc ccgccggcgc ccttgccgcg
    14041 cccgccgaaa cggccggggg cgaggatctt gcggccgatc aggccgatca tcaggatccc
    14101 gaacaggacc ccgaccaggc ggatgttgag gcgcatgtcg ccgttgagga tgtggctgaa
    14161 ctcgtgcgcg accacgccct gcagttcgtc gcggttgagc cggtcaagcg cgccgcgggt
    14221 gaccgcgacc accgcgtccg agctcgagta accggcggca aaggcgttga tcgccgcttc
    14281 gtgctccagc acgtagaccc tgggtaccgg caccccggac gcgatcgcga tttcctcgac
    14341 cacgttgcgc agccggcgca ggttgaggtc ggtggcatcc tccggcaccg gcaccccgcc
    14401 catctgcagg gcgaccgcgt cgccaccgcc gcgcaggctg gcgatccggt acatcgagcc
    14461 gaacccgatc accgccaggg tggcgacgct ggtgaacgcc accacctgca ccgaaccggt
    14521 cagcaaccag gcaaccgcat ccaccgcggc gacgatcccg gccaccgcca gcgcgaacag
    14581 caacaacagc cgcgaggaca tgcgccgcgc cgccgcctgg tgctcgaaga aattcatgca
    14641 ggtgcgtcct cgccgcaacc cctccgccgg cgggaggggc gtgtcaatcc gtcagaactg
    14701 caccttcggc gcggcgcgca cgtcggcctt ccgctccgcc gggatttcca gcagggtggc
    14761 ggcggtgaag ccgaacatgc cggcgaagat gctgttcggg aacatctcgc gccggttgtt
    14821 gtaggccatc accgcgtcat tgaacgcctg ccgcgcgaac gcgaccttgt tctcggtgct
    14881 ggtcagctcc tcggtcaact gcatcatgtt ctggttggcc ttgaggtccg gataggcctc
    14941 ggcgaccgcg agcaggcggc cgagcccggc attcaactgc ccttgcgcgg cgaccagctg
    15001 cgccatcgcc tccggatcgc ccgggctggc cttggccgcg gacaggccgg actgcgcggc
    15061 ggcgcgcgcg gcgatcaccg cctccagcgt ttcccgctca tggctcatgt acttgcgggc
    15121 cacttcgacc aggttcggga tcaggtcgaa gcggcgctgc agctgcacgt cgatctgcgc
    15181 gaaggcgttc ttgaacgcgt tgcgcgcggt caccaggccg ttgtagatgc ccacgcccca
    15241 gaatgcgacg gcggccacaa tcaccagcaa tatcagcagc gtaagcatca aagctcccct
    15301 cattcatgca atcgtggaaa caggcgctag aatcgggcgc aggtcggagc atacgcaggg
    15361 ggcaaggccg atgaaagaca ccagcactcg gcaggcttgg cgtatcgggc tggtggcggc
    15421 gctgctgccg ctggcccttg gttccgccgc gcaaagcgga ttgcagtgga aggcccagcc
    15481 ggcaccgtcg acggcggtgc aagcggctgc gaccggcgcg cctcctgcag gcaactcgca
    15541 agtggcagcg cgcccattcg acgtgcgcag cttcgagtcc atggcccagc aactggttgc
    15601 cggcgggcgc attcccgggc tggcggtcgc gatcgtgcga gacggccgcg tgctcagcgc
    15661 gcgcggctac ggggtcaccg acgcccgcaa cggcgagccg gtggacgcgc atacggtgtt
    15721 tcgcctcgcc tcgctgtcga agtcgttcgc cggcaccgtc accggcatgc tggtcaacga
    15781 cgggctgctg cgctgggaca gccggctgac cgactacctg ccggacttca ggctgtcgct
    15841 gccgaacgct gccgagcagg tcaccgtggc cgacctcctc agccaccgcg tgggcctcac
    15901 ccacaacgcg tacgaccgcg acctcgaggc aaacgcggac ttccatgcgc tgaccatgaa
    15961 gatggccaac gcgcccatga cctgcgcgcc cggccagtgc tatgcctacc agaacgtggc
    16021 attcagcctg gtcggcgacg tggtgttcgc cgccaccggc cagttctaca gcgaggaagt
    16081 ggcgcgccgc atcttcaagc cgctgggcat gcacgacgcc agctacggac tggaaggcat
    16141 cgaggccagt gcgcgctggg caaagccgca cgtgcgtggc ggcggcggct gggtgccgct
    16201 gaagccgaag cccaactact atcgcgtcgc gcccgcggcc ggcgtcaacg ccagcatcag
    16261 cgacatggcg cagtggctga tcgcgcagac cgggcaccgc ccggacgtcc tgccggcgcc
    16321 gctgctggcg accctgcatt caccgctggt gtccaccccc ggcgaattgc gcggcgcggc
    16381 ctggcgccgc acccgcctgg gtgccgccgg ttacgcactg ggctggcggg tgttcaacta
    16441 cgccggccac gacgtggtgt tccatggtgg cgcggtgcaa ggctatcgcg gcgcgatggc
    16501 gctgcttccg gatagcgacc tcggcgtcgc gatcctgtgg aacagcgaga gcgcgctgcc
    16561 gtccggcctg ttgccgacca tcctcgacag tgcgctgggc ctgaccggcg gccagtggct
    16621 ggacgacgag ctggcggcgc cggaaccggc gatgtacgcc aaccagccgg gtacgcaggc
    16681 gggcgtggaa gcctcggccg cgaacgcctc gccggaataa gccgcgggca aaaagaaacc
    16741 cctcccgcct gggcaacggg aagggccttc agtgtgcagc gactgcccgg ccgggtgctc
    16801 ccggccggtg cgtcttacat catcggtgca ggcatcgccg gcatcgcaac cggcttggcc
    16861 ttcttggcgg ccttcttctt ggcagccggc ttcttcgcag cagccttctt cgcagccggc
    16921 ttcttggcag cagccttctt taccgccttc ttggcggccg gcttcttcgc agcagccttc
    16981 ttcaccgcct tcttggcggc cggcttcttc gcagcagcct tctttaccgc cttcttggcg
    17041 gccggcttct tcgcagcagc cttct
//
LOCUS       DAORUK010000029        16911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_3117_length_16911_cov_19.464464, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000029 DAORUK010000000
VERSION     DAORUK010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 16911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16911
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3117_length_16911_cov_19.464464"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PK818_03290"
     CDS             complement(<1..620)
                     /locus_tag="PK818_03290"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HQY81790.1"
                     /translation="MTQSAKKPAVAVLGAGSWGTALAALIARHGHPTVLWGRDAATIA
                     AIEGEGENPRYLPGIPLPGGLRATTSLHEAMASAGLVLVVVPSHAFAETLRALAPLRP
                     PPAGVAWATKGFEPGSGRFLHEVAGEVLGDDVPLAVVTGPSFAKEVALGLPTALTVHS
                     DDPAFAQQTADVLHGPAFRAYTGDDMRGAELGGAMKNVLAVATGVA"
     gene            complement(660..1190)
                     /gene="secB"
                     /locus_tag="PK818_03295"
     CDS             complement(660..1190)
                     /gene="secB"
                     /locus_tag="PK818_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HQY81791.1"
                     /translation="MSDEILNGAAAESQAQPQGPSFTVEKLYVKDLSFEAPNAPQVFN
                     EQGQPDLQMKLSQKVNRLAEAAFEVSLGITITCTVNGKTAYLAEVEQAGVFGLAGFDE
                     QLLDAMLGTQCPNILYPYATAAIGQLITQGGFPPFPMQPINFDALYGESLRQRAAQIA
                     QQGGDGLANAETAGNA"
     gene            complement(1323..1760)
                     /locus_tag="PK818_03300"
     CDS             complement(1323..1760)
                     /locus_tag="PK818_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014645505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQY81792.1"
                     /translation="MTLAEFIAFAGRHPILSLALAGITLALLYNEIAGRLSGIRRIAP
                     AQLTALINRDNALVVDLRIAGDFEKGHIAGARNVQMSQFDPENKHLAPARALPVVLVC
                     KVGQTADGAAKRLRKAGFTNVNVLEGGIQAWQAADLPLVKGRG"
     gene            complement(1839..2258)
                     /locus_tag="PK818_03305"
     CDS             complement(1839..2258)
                     /locus_tag="PK818_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81793.1"
                     /translation="MTRLLLRCVLICCLALLAGCPKSASKGIALEEIQYAYSAAIRWG
                     DFEGAWNLVDPKARAEHPLTDIDFSRYKQVQISGYRDLGGTVTPEGVVMREIEIGVIN
                     RNTLTERSVRYRERWRYDEAAQTWWLVSGLPDLWADD"
     gene            complement(2341..2826)
                     /locus_tag="PK818_03310"
     CDS             complement(2341..2826)
                     /locus_tag="PK818_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HQY81794.1"
                     /translation="MDVPAKPGTALAALAAHFAAMPPVAALQPTVDAAGDGRLRLRAP
                     LAPNINDKGCAFGGSLVSLMTIAAWGLVFLELAQAGIEADIFVADSRVRYLKPVFEDI
                     VVEAGFDDPSERATLVGTLRRQGRASIRLKSESMLADGGVAASFVGRYVAIVRTTGQH
                     G"
     gene            2872..3651
                     /locus_tag="PK818_03315"
     CDS             2872..3651
                     /locus_tag="PK818_03315"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HQY81795.1"
                     /translation="MPRIANQALPLQDWCVLSLRPRNQHGGLRAAAARSGARTLALSP
                     FAIETRADAATRAALAQALGAEVVLYTSPNAVAAAAAIRAPKASRGQQVLAVGSGTRR
                     ALHRLGVAADAPGRMDSEGLLAMPQLAAVASRRIGLVTGAGGRDRLAPALRLRGAVVL
                     RADVYARVPLPLPPKRLDALAAVLADPRQVLLPVSSGEALQHVLVGLPAPLRKPFARI
                     AVVAASARLADVARAAGFRRIAIATDARPASLLRAAVDAFA"
     gene            3675..4646
                     /locus_tag="PK818_03320"
     CDS             3675..4646
                     /locus_tag="PK818_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003488502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81796.1"
                     /translation="MDPSPSPAPPTPPKRSLAGGLLLFLLFALVLAAAGWYGWRHWQQ
                     EQARIESLALQQRQALDARIDALRATQQAQAQRLQQAEATNRVLRDELLGIGQRAALL
                     EDSVSKLADPDRRGAQSLRLDEIELLLGIGQQRLRLAEDVDGARRALALAAPLVDGID
                     DPAYLNLRQTLAQEQAAMEALGADPRVRAQALLDALMGGLRAAPAATAGSTRPEPWYQ
                     RLLARVIRVQPTAGAGLQQRPDREAALAALQVEATLARAAIERRDGSALAAALARIDD
                     GLQRLLAGSAGLARKRRIVAELRALPLRADSPLSGTTLQQLRALRGQ"
     gene            4712..5962
                     /locus_tag="PK818_03325"
     CDS             4712..5962
                     /locus_tag="PK818_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HQY81797.1"
                     /translation="MNLFRSVLTWLVLAVLGALLAQLLLQDPGHVLVRFRGTDYSTTV
                     AVGVGILLAALFSLVLAWNLLRLPFRAWKRHRQRQARAKLSEGLSAYQRGEYARAEQL
                     LVQAADAGDAEAVARAHAARAAHARGDETAAAAHAEALGARHPAARAVLLAQQALARN
                     DAPAALAALDAPAAQPLPPRGLRLRGEALAATGRSFDAYGLLGALRQQQALPAAQLDA
                     LQAQWAAAALQQAGDANALADRWDALPQTLRNTADVVAAYARRAAALRWDEAATRAIE
                     QALDARWSDTLADLYGRLPVSRLDQRRAQVERWLSAHPSSPALLLAAARIAQAQGQWP
                     QADRWLHRAIAQGAGAPAWEALGDGALNAGDETRARLAYANALRSQRGDALLDLPGRD
                     LRQQIADTAAIEERDAHGLPRLRE"
     gene            6003..6917
                     /gene="hemF"
                     /locus_tag="PK818_03330"
     CDS             6003..6917
                     /gene="hemF"
                     /locus_tag="PK818_03330"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007967076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HQY81798.1"
                     /translation="MPSIDTVRDYLTGLQDRICTAIELADGGARFVEDAWTRAEGGGG
                     RTRILREGAVFEQAGIGFSDVSGTQLPPSATAARPELAGASWRAVGVSLVFHPRNPYL
                     PTTHANVRHFRAMRDGETVAWWFGGGFDLTPFYPFDEDVLHWHRVARSLCEPFGGQAR
                     YEAHKRWCDEYFFLRHRNETRGVGGLFFDDLHGDFETDFGYLRAVGDGFLAAYLPIVQ
                     RRKDTPWGERERQFQLYRRGRYVEFNLVLDRGTLFGLQSGGRTESILMSLPPLVRWEY
                     GYAPDAGSDEARLAEYLRPRDWVDAPQE"
     gene            complement(7136..9901)
                     /gene="polA"
                     /locus_tag="PK818_03335"
     CDS             complement(7136..9901)
                     /gene="polA"
                     /locus_tag="PK818_03335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HQY81799.1"
                     /translation="MPKLVLIDGSSYLYRAFHALPPLTNAAGEPTGALFGVVNMLRAH
                     LKEKPEFAAFVIDAPGRTFRDDLDPQYKANRPPMPDELRAQVEPMLQIVQALGFPILR
                     VDGVEADDVIGTLAVQGARAGIDVTISTSDKDFAQLVRPGVALVNTMSGGKLDSDAAV
                     VEKFGVRADQIIDFLALMGDSVDNIPGVPKCGPKTAAKWLAEYDTLDGVIANAGNIGG
                     KIGESLRETLPRLPLNRELTTIKTDVALEQAPDNLPLRERDAGALRALYMRYGFNQAL
                     KELDGGAAAAPAADKEPGVARGNGFVAASAPAAPADIDPALSAPGDYECVLTTAQLEA
                     WVAKLQAAEAFAFDTETDSLDPLRANLVGISLCVEPGKACYIPVAHEAPGVAAQLPRD
                     AVLDALRPLLADPAKKKLGQHGKYDLHVLRRHGVVVAGYADDTLLESFVLNAGIARHD
                     MDSLAQRHLGYTTIKYEDVAGKGSKAIAFSQVALDDATRYAAEDADITLRLHRVLLPK
                     LQAEPGLLSVYRDIEMPLVPVLARIEANGVMIDADELRRQSADLSRRMLAAQQKATEL
                     AGRSFNLDSPKQLGALLFDELKLPALVKTPGGAPSTNEEALEAIADQHELPRVILEYR
                     GLAKLRSTYTDKLPEMVNPDTGRVHTSYHQAGAATGRLSSSDPNLQNIPIRSEEGRRI
                     RTAFVAPTGRKLVACDYSQIELRIMAHLSGDAALVRAFESGADVHRATAAEVFGKPLD
                     EVSGNERRAAKAINFGLMYGMGAFGLARQLGIGRGEAQDYIALYFSRYPGVRDFMERT
                     RQQARERGYVETVFGRRLALDYIHSRNAAQRAGAERAAINAPMQGTAADIIKRAMVDI
                     DAWLAGQGGRALMLMQVHDELVFEVDEAFLPTLLAQVPARMAAAATLRVPLVVDTGVG
                     DNWDQAH"
     gene            9983..10264
                     /locus_tag="PK818_03340"
     CDS             9983..10264
                     /locus_tag="PK818_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HQY81800.1"
                     /translation="MRIALPSVLAVVLAVAACSTVPPEDMLANAEPTTRTEANGDTVT
                     EYRVAGALKMVKVVPFRGPTYYIHDRDGDGKLDWGKGEAPLTYYKLYSW"
     gene            complement(10269..10925)
                     /locus_tag="PK818_03345"
     CDS             complement(10269..10925)
                     /locus_tag="PK818_03345"
                     /inference="COORDINATES: protein motif:HMM:NF018264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HQY81801.1"
                     /translation="MSRDTDRLIEQLALEVVPVRPLASPMQRTVGWLAASMAVVAVLA
                     VLLGWRGGMPADMAVPGGGLAWWGSLLTGIVAAHAVFQVSVPGRSPGWAWLALPPALA
                     WFAGMGWGCLAEARAGTLAFGLESWHCALMITVVSTPMLLVMLLLVRHAGVLRPVPTA
                     ALAVLSAAGLSSAAVSLVHGQESAALTLLWHGGAVLLLCALAALSGRPLLGWIGYARR
                     "
     gene            complement(10915..11511)
                     /locus_tag="PK818_03350"
     CDS             complement(10915..11511)
                     /locus_tag="PK818_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQY81802.1"
                     /translation="MPTDADAAPQADPSPDDWGTWMALAQAGDRQAYQALLQAIGPYL
                     RAIARRLLGHEDVEDAVQEILLVVHGIRHTYQPGRPFKPWLATIASRRCIDLLRRRQR
                     EGLRLGEALDEEAHMDAEPGPAERLERAQQDARMRAAVGRLSPGQRDAVRLLHLQELS
                     LREAEASGGQSAGALKVASHRALKALRRALQGEHDHVP"
     gene            complement(11544..12032)
                     /locus_tag="PK818_03355"
     CDS             complement(11544..12032)
                     /locus_tag="PK818_03355"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQY81803.1"
                     /translation="MSTTFARHAAPVASSWVAEAIGWLARIGLAFTFWLSGQTKIEGL
                     VLDPLALQVQWGWPQVSASALELFRSEYALPLLPPEWAALLAAGAEHVLPLLLLLGLG
                     TRLAALGILGMTLVIQVFVYPLAFPTHALWAAAALYLLAHGGGRVALDRLLAGRLRQQ
                     GA"
     gene            complement(12029..12820)
                     /locus_tag="PK818_03360"
     CDS             complement(12029..12820)
                     /locus_tag="PK818_03360"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HQY81804.1"
                     /translation="MNGHADFAAGLLRPERPVPQALHAADPQAREHRYAVYRNNTTSA
                     LIAAFAAAYPVLRALLGVDGFAHLARECARATPPRTPVLAEYVQVLPGFLATTPLPAT
                     LPYLVDVARLEAACLRVFHAADADALPAAAWHALQADPQRLGQARVLLHPACAWLACT
                     HAAADLWLAHMRAAHPELAELDQVDAYAAQDALAWRDPTGRVQVDALPSGCALALDAL
                     QRGAPLLQAVAALSPQACAALLAHLAGDGLAVAVQPDPDGLAEVS"
     gene            complement(12817..13704)
                     /locus_tag="PK818_03365"
     CDS             complement(12817..13704)
                     /locus_tag="PK818_03365"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HQY81805.1"
                     /translation="MRASATLPRAGAPGPGLPMQAGIGFKPEHFDALMADPAPPAFVE
                     VHAENYFGDGGLPHRQLAALCGRTALSVHGVGLSIGGHDPLDRTHLQRLRRLLERHPA
                     AQFSEHLAWSSHDGVHYPDLLPLRYDDAGLRRVCSHVGEVQDLLGRRMLLENPSTYLE
                     FEAADHAEAGFLAEVVRRSGCGLLLDVNNAYVSCRNHGRDVRTYLAGLPLHAVGEIHL
                     AGHATVADHDGGHLLVDDHGAPVADAVWSLYREVLAQVGLVPTLVEWDTDVPDYPVLR
                     AQAALADACLRDAATAVAA"
     gene            complement(13713..14039)
                     /locus_tag="PK818_03370"
     CDS             complement(13713..14039)
                     /locus_tag="PK818_03370"
                     /inference="COORDINATES: protein motif:HMM:NF021559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HQY81806.1"
                     /translation="MSDLRTPALTAAALAAVLGSALLGAVTPAQAGDKAMAKEKCYGV
                     AMAGKNDCAAGPGTSCAGTSKSDHQANAWKYVAAGTCTKTASKTSPTGKGQLAAFEAT
                     MPAKAM"
     gene            complement(14145..16349)
                     /gene="uvrD"
                     /locus_tag="PK818_03375"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(14145..16349)
                     /gene="uvrD"
                     /locus_tag="PK818_03375"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HQY81807.1"
                     /translation="MDVSHLLDGLNPAQREAVSAAPGHYLVLAGAGSGKTRVLTHRIA
                     WLHEVFGVPMHGILAVTFTNKAAGEMRARIAQQLGGEPRGMWVGTFHGIAHRLLRLHW
                     QDAKLPESFQVLDSDDQQRLVKRVAQALEIDEARFPPRQIAWWINAQKDEGRRPQHIQ
                     PEADEWGSVMLRAYTEYQLRCERAGLVDFAELLLRAHEVLRDTPALLAHYRHRFGQLL
                     VDEFQDTNAIQYGFVRLLAGETGQAASGHVFVVGDDDQAIYGWRGAKVENVQRFLRDY
                     PGAVTLKLEQNYRSSANILNAANAVIAHNDDRLGKQLWTDAGEGEPIDVYAAYNEVDE
                     ARYAIERIAQWVRDGGSHGEAAILYRSNAQSRAFEEALLAAQIPYRVYGGMRFFERAE
                     IKDTLAYLRLVANRDDDAAFERAVNTPTRGIGGRTLDEVRLCAREHGVSLWGAVQKLC
                     TQSVLAARARNALAAFERLIDALAVETASMPLQEKIDHVLARSGLRLHYENESRGQLD
                     SRTDNLDELVSVASRFRRGDDEDAAQMSELVAFLSYASLEAGEGQAQAGADGVQLMTL
                     HSAKGLEFPLVFLAGLEEGLFPNMRSAEEPGRMAEERRLAYVGITRARQQLVLSHAET
                     RRIHGSDMYGLPSRFLREIPKALLREIRPKPQVARGGYAMQANRFTPRDRGHAALEAS
                     PVRLGANVRHPTFGTGFVTDVEGSGAHARVQVNFEDAGSKWLVLAYANLTPA"
     gene            complement(16475..>16911)
                     /locus_tag="PK818_03380"
     CDS             complement(16475..>16911)
                     /locus_tag="PK818_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HQY81808.1"
                     /translation="LGLIDVVMISNLLMMVIVGGYETFVSRMRLERHPDQPEWLSHVN
                     ASVLKVKLAMAIIGISSIHLLKTFIEAGTLDGMPLCGTPVAFAAAKDALAAGYAGVTC
                     NKVTATGVMWQTIIHTIFILSALGIAWTDKVMSATPGKRAAH"
BASE COUNT         2421 a         6043 c         5986 g         2461 t
ORIGIN      
        1 tcggccacgc cggtggccac cgccagcacg ttcttcatcg ccccgcccag ctcggcgccg
       61 cgcatgtcgt cgccggtgta ggcgcggaac gcggggccgt gcagcacgtc cgccgtctgc
      121 tgggcgaagg ccggatcgtc cgaatgcacg gtcagcgcgg tgggcaggcc cagcgcgact
      181 tccttcgcga acgacgggcc ggtcaccacc gccagcggca cgtcgtcgcc aagcacctcg
      241 ccggcgactt cgtgcaggaa gcgcccgctg ccgggctcga agcccttggt cgcccacgcc
      301 accccggccg gtggcgggcg cagcggcgcc agcgcgcgca gggtctcggc gaaggcatgc
      361 gaagggacca ccaccagcac caggccggcg ctggccatcg cttcatgcag ggaggtggtg
      421 gcacgcaggc caccgggcaa cgggatgccc ggcaggtagc gcgggttctc gccctcgccc
      481 tcgatcgcgg cgatggtggc cgcatcgcgg ccccacaaca cggtggggtg gccgtggcgg
      541 gcgatcaacg cggcgagcgc ggtgccccag gagcccgcgc ccagtaccgc gaccgccggt
      601 tttttggctg actgcgtcat cgtgctgttt ggcatgactt gcgccatgcg gccggcagct
      661 tacgcgttgc cggcggtttc cgcgttggca aggccgtcac cgccctgctg cgcgatctgc
      721 gcggcacgct ggcgcaggct ttcgccatac agcgcgtcga aattgatcgg ctgcatcggg
      781 aacggcggga agccgccctg ggtgatcagc tggccgatcg cggcggtggc gtacggatac
      841 aggatgttcg ggcactgggt gccgagcatc gcgtccagca gttgctcgtc gaaaccggcc
      901 aggccgaaca cgcccgcctg ctcgacttcc gccaggtacg cggtcttgcc attcacggtg
      961 caggtgatgg tgatgcccag gctgacctcg aacgccgctt cggccaggcg attgaccttc
     1021 tggctcagct tcatctgcag gtccggctgg ccctgttcgt tgaacacctg cggcgcgttc
     1081 ggggcctcga acgacaggtc cttcacgtac agcttctcga cggtgaagct cgggccctgc
     1141 ggctgcgctt gggattcggc ggcggcgccg ttcaggattt catcggacat gggtggctcc
     1201 agcaaatgcg ttgcgtgcgg aaaaaggggc gggaaagcaa gccgcggatt atcgcaccga
     1261 atgcaacggc aggtcggtgc gtcggttcag acgtggggtg cgcggacggc gcttgcaagg
     1321 gctcagccgc gtcccttgac cagcggcagg tcggcggcct gccaggcctg gatgccgccc
     1381 tcgagcacgt tgacgttggt gaagccggcc ttgcgcaggc gcttggcggc gccgtccgcg
     1441 gtctgcccga ccttgcatac cagcaccacc ggcagcgccc tggccggggc caggtgcttg
     1501 ttctcggggt cgaactggct catctgcacg ttgcgggcgc cggcgatgtg gcccttctcg
     1561 aagtcgccgg cgatccgcag gtccaccacc agcgcgttgt cgcggttgat cagcgcggtc
     1621 agctgggccg gcgcgatccg gcggatgccg gacaggcggc cggcgatctc gttgtagagc
     1681 agggccaggg tgatgccggc cagcgccagc gacaggatcg ggtggcggcc ggcgaaggcg
     1741 atgaattcgg cgagggtcac ggggtcgacc gggtcatgca gggccggcga ttgtagccaa
     1801 tgccggcgtc gccaacggtg gcgacgccga agcctgcgtc agtcgtcggc ccacaggtcc
     1861 ggcaggccgc tgaccagcca ccaggtctgg gcggcctcgt cgtagcgcca gcgctcgcgg
     1921 tagcggacgc tgcgttcggt cagggtattg cggttgatca ccccgatctc gatctcgcgc
     1981 atcaccacgc cctccggcgt caccgtgccg ccgagatcgc ggtaaccgga gatctgcacc
     2041 tgcttgtagc gcgagaaatc gatgtccgtc agcggatgtt ccgcgcgcgc cttcgggtcg
     2101 accaggttcc acgcaccctc gaaatcgccc cagcggatcg cagccgagta ggcgtactgg
     2161 atttcctcca gcgcgatgcc cttgctcgcg gatttcgggc agccggccag cagcgccagg
     2221 cagcagatca ggacgcatcg cagcagcagt cgggtcatcg cacgtgctcg gccgggggac
     2281 gccgcatcct accgcgagcg gccgcggggc cgctgcaccc gcgccggtcc gccgccagcg
     2341 ctagccgtgc tgcccggtgg tgcggacgat cgccacgtag cggccgacga aactggcggc
     2401 caccccgcca tcggcaagca tcgactccga cttcaggcgg atgctggcgc ggccctgcct
     2461 gcgcagggtc ccgaccaggg tcgcgcgctc ggacgggtcg tcgaagccgg cctcgaccac
     2521 gatgtcctcg aacaccggct tcaggtaccg cacccggctg tcggcgacga agatgtcggc
     2581 ttcgatgccg gcctgcgcca gctccaggaa caccagcccc caggcggcga tggtcatcag
     2641 cgacaccagg ctgccgccga acgcgcagcc cttgtcgttg atgttgggcg ccagcggcgc
     2701 gcgcagccgc agccggccgt cgcccgccgc gtccacggtg ggctgcagcg ccgcgaccgg
     2761 cggcatcgcg gcgaagtgcg cggcgagggc ggcgagggcg gtgcctggtt tcgcgggtac
     2821 gtccatgccc gaagcctgac gcaaaccccg aggccatgcc aagctggcgg catgccgcgc
     2881 attgcgaatc aagcacttcc acttcaagac tggtgcgtac tgtcgctgcg tccgcgcaac
     2941 cagcatggcg gtctgcgcgc ggcggcggcc cgatccggcg cgcgcacgct ggcgctgtcg
     3001 ccgttcgcga tcgaaacccg cgccgatgcc gcgacccgcg ccgcgctggc gcaggcactg
     3061 ggcgccgaag tggtgctcta caccagcccg aatgcagtcg ccgcggcggc ggcgatccgg
     3121 gcacccaagg caagccgcgg acagcaggta ctggcggtcg gcagcggcac ccgccgtgcg
     3181 ctgcaccggc tcggcgttgc cgccgacgcg cctggccgga tggacagcga aggcctgctg
     3241 gcgatgccgc aactcgccgc tgtcgccagc cgccggattg gcctggtgac cggtgccggc
     3301 ggacgcgatc gcctggcgcc cgcgcttcgc ctgcgcggcg ccgtggtgtt gcgggcggac
     3361 gtgtatgcgc gcgtgccgct gccgttgccg cccaagcggc tggacgcgct cgccgcggtg
     3421 ctcgccgatc cgcggcaggt gttgctgccg gtgagcagcg gcgaggccct gcagcacgtc
     3481 ctggtcggcc tgcccgcgcc gctgcgcaag ccgttcgcgc ggatcgccgt ggtcgcggcc
     3541 agtgcgcggc tcgcggacgt cgcgcgcgcg gccggtttcc gccgcatcgc catcgccacc
     3601 gatgcgcgcc cggcgtcgtt gctgcgcgcc gcggtcgacg ccttcgcctg acggccatcg
     3661 tttaggattg gccggtggat ccatcgcctt cccccgcccc gcccacgcca ccgaaacgct
     3721 cgctcgccgg cgggctgctg ctgttcctgc tgttcgcact ggtgctggcg gcggcgggct
     3781 ggtacggctg gcggcattgg cagcaggagc aggcacgcat cgagtccctg gctttgcagc
     3841 agcggcaggc gctggatgcc cgcatcgatg ccctgcgcgc aacccagcag gcgcaggcgc
     3901 agcgactgca gcaggcggag gcaaccaacc gggtgctgcg cgacgaactg ctcggcatcg
     3961 gccagcgcgc ggcgctgctg gaagacagcg tgtcgaagct ggccgacccc gaccgccgcg
     4021 gcgcgcaatc gctgcggctg gacgagatcg aactgctgct cggcatcggc cagcaacgcc
     4081 tgcggctggc cgaagacgtg gatggtgcgc gccgcgcgct ggcgctggcg gcgccgctgg
     4141 tggacggcat cgacgacccg gcctacctca acctgcgcca gaccctggcc caggaacagg
     4201 cggcgatgga ggcgttgggc gcggatccgc gggtgcgcgc acaggcgttg ctggacgcgc
     4261 tgatgggcgg cctgcgcgct gcgcctgcgg cgaccgccgg cagcacccgc ccggagccgt
     4321 ggtaccagcg cctgctggcg cgggtgatcc gggtgcagcc caccgccggt gccggcctcc
     4381 agcaacgccc cgaccgcgaa gccgcgctgg cggcactgca ggtcgaggcc acgctggcgc
     4441 gcgcggcgat cgagcgccgc gacggcagcg cgctggccgc cgcgctggca cgcatcgacg
     4501 acggcctgca acggctgctt gcggggtcgg cggggctcgc gcggaagcgc aggatcgtcg
     4561 ccgaattgcg ggcgctgccg ctgcgcgcgg attcgccgct gtcgggcacc acgttgcagc
     4621 agctgcgcgc attgcgcggg caatgaccca cccacgccgc gatggcgtcg cgcccgcgat
     4681 gctgcgcggg taacccacca ccggaggcga catgaacctg ttccgcagcg tgctgacctg
     4741 gctggtgctg gcggtgctgg gggcgctgct ggcgcagctg ctgctgcagg atccggggca
     4801 cgtgctggtg cgcttccgcg gcaccgacta cagcaccacc gtcgcggtcg gcgtcggcat
     4861 cctgctggcc gcgctgttct ccctggtgct ggcctggaac ctgctgcggc tgccgttccg
     4921 cgcgtggaag cggcaccggc aaaggcaggc acgggcgaag ctcagcgaag gcctgtccgc
     4981 ctaccagcgc ggcgagtacg cgcgcgccga gcagttgctg gtgcaggccg cggatgcggg
     5041 cgatgccgaa gcggtggcgc gcgcgcatgc ggcacgcgcc gcacacgcgc gcggcgacga
     5101 gaccgcggcc gccgcgcatg ccgaagcgct gggcgcacgc caccccgccg cccgggcggt
     5161 gctgctcgcc cagcaggcac tggcgcgcaa cgatgccccc gccgcgctgg cggcactgga
     5221 tgcccccgcg gcgcaaccgc tgccaccgcg cggcctgcgc ctgcgcgggg aggcactggc
     5281 ggcaaccggc cgcagcttcg acgcctatgg cctgctcggc gcgctgcgcc agcagcaggc
     5341 gctgccggcg gcgcaactcg acgcactgca ggcgcagtgg gccgcggcgg cgctgcagca
     5401 ggccggcgat gcgaacgcgc tggcggaccg ctgggacgca ctgccgcaaa cgctgcgcaa
     5461 caccgccgac gtggtcgccg cctatgcccg gcgcgccgcc gcgctgcgct gggacgaagc
     5521 ggcgacgcgc gcgatcgaac aggcgctgga cgcacgctgg agcgacaccc tggccgacct
     5581 gtacggacgg ctgccggtct cccgcctcga ccagcgccgc gcgcaggtcg aacgctggct
     5641 gtccgcgcat ccgtccagcc ccgcgctact gctggccgcc gcgcgcatcg cccaggccca
     5701 gggccaatgg ccgcaggccg accgctggct gcaccgcgcc atcgcccagg gcgccggcgc
     5761 accggcctgg gaagcgctgg gcgacggcgc gctcaacgcg ggcgacgaaa cccgcgcccg
     5821 ccttgcctac gcgaatgccc tgcgcagcca acgcggcgac gccttgctgg acctgccagg
     5881 ccgcgacctg cgccagcaga tcgccgatac cgcggcgatc gaggagcgcg acgcgcacgg
     5941 gttgccgcga ttgcgcgaat gagcgcttgg atgcgcgcac atacaatgcg cgcatgacgc
     6001 ccatcccctc catcgacacc gtccgcgact acctcaccgg cctgcaggac cgcatctgca
     6061 cggcgattga gttggcggac ggcggcgcgc gcttcgtcga ggatgcatgg acgcgcgccg
     6121 aaggcggcgg cgggcgcacc cgcatcctgc gcgagggggc ggtgttcgag caggccggca
     6181 tcggcttctc cgatgtctcc ggcacgcagt tgccgccgtc ggcgaccgcc gcgcgcccgg
     6241 agctcgcggg cgcctcgtgg cgcgcggtcg gcgtgtcgct ggtgttccac ccgcgcaatc
     6301 cctacctgcc gaccacgcat gccaacgtgc gccatttccg tgcgatgcgc gatggcgaaa
     6361 ccgtcgcgtg gtggttcggt ggcggcttcg acctgacccc gttctacccg ttcgatgagg
     6421 acgtgctgca ttggcatcgg gtcgcgcgct cgctgtgcga accgttcggc ggccaggcgc
     6481 gctacgaagc ccacaagcgc tggtgcgacg agtatttctt cctcaggcac cgcaacgaga
     6541 cgcgcggcgt cggcggcctg ttcttcgacg acctgcacgg cgatttcgaa accgacttcg
     6601 gctacctgcg cgcggtcggc gacggtttcc tggccgccta cctgccgatc gtccagcgcc
     6661 gcaaggacac cccgtggggc gagcgcgaac gccagttcca gctgtaccgg cgcggccgct
     6721 acgtcgagtt caacctggtg ctggaccgcg gcaccctgtt cggcctgcag agtggcggcc
     6781 gcaccgaatc gatcctgatg agcctgcccc cgctggtgcg ctgggaatac ggctacgcgc
     6841 cggacgccgg cagcgacgag gcgcggctgg cggagtacct gcggccgcgc gactgggtcg
     6901 acgcgccgca ggaatgaaaa agccccgcaa cctggggggg gctgcggggc cggggttgga
     6961 acggacggtt ggggagaacc tggccgttcc tggatcacac caggggaggg gtagtgatcg
     7021 gcgacagacg ttgcatctgt cgcgagtgat atgaccaccc tgcgccttga cggttcgtga
     7081 agattcgcgt taaagaactg ttcggttcag gaattattca tccgcgcgcg accggtcagt
     7141 gcgcctggtc ccagttgtcg ccgacgccgg tatccaccac cagcggcacc cgcagcgtgg
     7201 cggcggccgc catccgggcc ggcacctggg ccagcagggt cggcaggaag gcctcgtcga
     7261 cctcgaacac cagttcgtcg tgcacctgca tcagcatcag cgcgcgcccg ccctgcccgg
     7321 ccagccaggc atcgatgtcg accatcgcgc gcttgatgat gtccgccgcg gtgccctgca
     7381 tcggcgcgtt gatcgcggcg cgttcggcgc ctgcacgctg cgcggcgttg cgcgaatgga
     7441 tgtagtccag cgccagccgg cggccgaaca cggtttccac gtagccacgt tcgcgcgcct
     7501 gctggcgggt gcgttccatg aagtcgcgca cgccgggata gcggctgaag tacagcgcga
     7561 tgtagtcctg cgcctcgccg cggccgatgc ccagctggcg cgccagcccg aatgcaccca
     7621 tgccgtacat cagcccgaag ttgatcgcct tggccgcgcg acgctcgttg ccgctgacct
     7681 catccagcgg cttgccgaac acttccgccg cggtcgcgcg gtgcacgtcg gcgccgcttt
     7741 cgaacgcgcg caccagcgct gcgtcgccgg acaggtgggc catgatccgc aactcgatct
     7801 gcgagtagtc gcaggccacc agcttgcggc cggtgggggc gacgaaggcg gtgcggatgc
     7861 gccggccttc ctcgctgcgg atcgggatgt tctgcaggtt cggatcggac gaagacagcc
     7921 gcccggtggc cgctcccgcc tggtggtaac tggtgtgcac gcggccggtg tccgggttca
     7981 ccatctccgg cagcttgtcg gtgtaggtgc tgcgcagttt cgccaggccg cggtattcca
     8041 ggatcacccg cggcagttcg tgctggtcgg cgatcgcttc cagcgcttcc tcgttggtcg
     8101 agggcgcgcc gcccggggtc ttcaccagcg ccggcaactt gagttcgtcg aacagcagcg
     8161 cgccgagctg cttgggcgaa tccaggttga agctgcgccc ggccagttcg gtggctttct
     8221 gctgcgcggc cagcatgcgc cgcgacaggt cggcggactg ccggcgcagt tcgtcggcgt
     8281 cgatcatcac gccgttggcc tcgatccgcg ccagcaccgg aaccagcggc atctcgatgt
     8341 cgcgatagac cgacagcagg cccggttccg cctgcagctt cggcagcagc acgcgatgca
     8401 ggcgcagggt gatgtcggcg tcttccgccg cgtagcgggt ggcatcgtcg agcgcgacct
     8461 gcgagaacgc gatcgccttg ctgcccttgc cggccacgtc ctcgtacttg atcgtggtgt
     8521 agccgaggtg gcgctgcgcc agcgaatcca tgtcgtgccg cgcgatgccg gcgttgagca
     8581 cgaagctctc cagcagggtg tcgtcggcat agcccgcaac cacgacgcca tgccggcgca
     8641 gcacgtgcag gtcgtacttg ccgtgttggc cgagcttctt cttcgccgga tccgccagca
     8701 gtgggcgcag ggcatccagc accgcatcgc gcggcaattg cgcggccacg ccgggcgctt
     8761 cgtgggcgac cgggatgtag caggccttgc ccggctccac gcacaggctg atgccgacca
     8821 ggttcgcccg caacgggtcc agcgaatcgg tttcggtgtc gaaggcgaac gcttccgccg
     8881 cctgcagctt cgccacccag gcttccagct gcgccgtggt cagcacgcat tcgtagtcac
     8941 cgggcgcgga cagcgcggga tcgatgtccg ccggcgcggc aggcgcgctg gcggcgacga
     9001 aaccgttgcc gcgcgccacg ccgggctcct tgtccgccgc cggcgcggcg gccgcgccgc
     9061 catcgagctc cttcaacgcc tggttgaagc cgtagcgcat gtacagcgca cgcagggcac
     9121 cggcatcgcg ctcgcgcagc ggcaagttat ccggcgcctg ctccagcgcg acatcggtct
     9181 tgatggtggt cagttcgcgg ttcagcggca gccgcggcag ggtctcgcgc aggctctcgc
     9241 cgatcttgcc gccgatgttg ccggcattgg cgatcacgcc gtccagggtg tcgtattcgg
     9301 caagccattt cgccgcggtc ttcggcccgc acttgggcac gccggggatg ttgtcgacgc
     9361 tgtcgcccat cagcgccagg aaatcgatga tctggtcggc gcgcacgccg aatttctcga
     9421 ccaccgccgc gtccgaatcg agcttgccgc cgctcatggt gttgaccagc gccaccccgg
     9481 gccgcaccag ctgggcgaag tccttgtcgc tggtggaaat ggtgacgtcg atgccggcgc
     9541 gcgcgccctg caccgccagc gtgccgatca cgtcatcggc ttccacgcca tcgacgcgca
     9601 ggatcgggaa gcccaatgcc tgcacgatct gcagcatcgg ttcgacctgc gcgcgcagtt
     9661 cgtccggcat cggcgggcgg ttcgccttgt actgcgggtc gaggtcgtcg cggaaggtcc
     9721 tgccgggcgc gtcgatcacg aacgcggcga actccggctt ctccttcagg tgcgcgcgca
     9781 gcatgttgac cacgccgaac agcgcgccgg tcggttcgcc ggccgcgttg gtgagcggcg
     9841 gcaacgcgtg gaaggcgcga tacaggtagg aggaaccgtc gatcagcacg agtttcggca
     9901 tgccgcgatt gtacggggcg ctgccggacc cgcggccgcg tgcttcccgc atactgggcg
     9961 catcccgcac cggagccgcg ccatgcgcat cgccttgccg tccgtcctcg ccgtggtcct
    10021 cgccgtcgca gcctgcagca cggtgccgcc ggaagacatg ctggcgaacg cggagccgac
    10081 cacccgcacc gaagccaatg gcgacaccgt caccgaatac cgggtggccg gcgcgctgaa
    10141 gatggtcaag gtggtgccgt tccgtggccc gacctactac atccacgacc gcgacggcga
    10201 cggcaagctg gactggggca agggcgaagc cccgctgacc tactacaagc tgtacagctg
    10261 gtagccgctc agcggcgcgc gtagccgatc cagcccagca gcgggcgccc ggacaacgcg
    10321 gccagcgcgc acagcagcag gaccgcgccg ccgtgccaga gcagggtcag cgcggcgctc
    10381 tcctggccgt gcaccaggct gaccgccgcc gacgacaggc cggccgcgct cagcaccgcc
    10441 agcgccgcgg tcggcaccgg ccgcagcacg ccggcgtggc ggaccagcaa cagcatcacc
    10501 agcagcatcg gcgtactgac aacggtgatc atcagcgcgc agtgccaact ctccaggccg
    10561 aacgccaggg tgccggcacg cgcctccgca aggcagcccc aacccatgcc ggcgaaccag
    10621 gccagtgccg gcggcagcgc cagccaggcc cagcccggcg agcgccccgg cacgctgacc
    10681 tggaacaccg catgcgcggc gacgatcccg gtcagcaggc tgccccacca cgccagcccg
    10741 ccgccgggca ccgccatgtc cgcgggcatg ccgccgcgcc agcccagcaa caccgcgagc
    10801 accgcgacca ccgccatgct ggcggccagc cagcccacgg tgcgctgcat gggcgacgcc
    10861 agcggccgca ccggcactac ctccagcgcc aactgttcga tcaggcgatc ggtgtcacgg
    10921 gacatggtcg tgttctcctt gcagtgcgcg gcgcaacgcc ttcaacgcac gatggcttgc
    10981 gactttcagg gcgccggcgg attgcccgcc gcttgcttcg gcttcgcgca gcgacagttc
    11041 ctgcaggtgc agcaggcgca ccgcgtcgcg ctgcccgggg ctgaggcgtc ccaccgcagc
    11101 gcgcatccgc gcatcctgct gcgcgcgttc gagccgttcg gcaggcccgg gctctgcgtc
    11161 catgtgggcc tcctcgtcca gcgcttcgcc caaccgcagg ccctcgcgct ggcgacgccg
    11221 caacaggtcg atgcaacggc ggctggcgat ggtggccagc cacggcttga acgggcgccc
    11281 cggttggtag gtgtggcgga taccgtggac caccagcagg atctcctgca ccgcgtcctc
    11341 gacatcctcg tggccgagca ggcgccgcgc gatcgcgcgc aggtacgggc cgatcgcctg
    11401 cagcaaggcc tggtaggcct ggcggtcgcc ggcctgcgcc agcgccatcc acgtccccca
    11461 gtcatcgggc gatggatcgg cttgcggggc ggcgtcagcg tcggtcggca cgggacacgc
    11521 cgggtggcgg aggacgacgc ccatcatgca ccctgctgcc ggagccgccc ggccagcagg
    11581 cgatcgaggg ccacccggcc accgccgtgg gcgagcaggt agagcgcggc ggcggcccac
    11641 agcgcgtggg tcgggaacgc cagcggatac acgaacacct ggatcaccag ggtcatgccg
    11701 aggatgccca gtgcggccag ccgcgtgccc agtcccagca gcaacagcag cggcagcaca
    11761 tgctccgcgc cggcggcgag caacgccgcc cactccggcg gcaacagcgg caacgcgtat
    11821 tcgctgcgga acagttccag cgccgacgcg ctgacctgcg gccagcccca ctgcacctgc
    11881 agcgccagcg gatccagcac caggccctcg atcttggtct gcccggacag ccagaacgtg
    11941 aacgccaggc cgatgcgcgc gagccagccg atggcttccg ctacccagct cgaagcgacg
    12001 ggcgcggcat gccgtgcaaa tgtcgtactc atgacacctc cgcgaggccg tctggatcgg
    12061 gttgcacggc aaccgccagg ccatcgccgg ccaggtgcgc cagcaacgcg gcgcaagcct
    12121 gcggggacaa cgccgccact gcctgcagca gcggtgcgcc gcgctgcagc gcatccagcg
    12181 ccagcgcgca accgctcggc agcgcgtcga cctgcacccg ccccgtcggg tcgcgccagg
    12241 ccagcgcgtc ctgcgcagcg taggcatcga cctggtccag ttcggccagt tccgggtgcg
    12301 ccgcgcgcat gtgcgcaagc cacaggtcgg ccgccgcatg ggtgcaggcc agccatgcgc
    12361 aggccgggtg cagcaacacg cgcgcctgcc caagccgctg cggatccgcc tgcagcgcgt
    12421 gccaggctgc ggccggcaag gcgtcggcat cggcggcgtg gaacacgcgc aggcaggccg
    12481 cttccagccg ggcgacatca accaggtacg gcagtgtcgc cggcagcggg gtggtggcga
    12541 ggaagccggg caagacctgc acgtattcgg ccagcaccgg cgtgcgcggc ggggttgcgc
    12601 gcgcgcattc gcgcgcgagg tgggcgaagc cgtccacgcc cagcagcgcg cgcagcaccg
    12661 gataggccgc ggcgaacgcg gcaatcaggg cactggtcgt gttgttgcga tagaccgcgt
    12721 agcgatgctc gcgcgcctgc ggatccgcgg catgcagcgc ctgcgggacc ggccgctcgg
    12781 gtcgtagcag gccggcggcg aaatcggcgt gtccgttcat gccgccaccg ccgtcgctgc
    12841 gtcgcgcagg caggcatcgg caagcgccgc ctgtgcgcgc agcaccgggt aatcgggaac
    12901 gtcggtgtcc cattcgacca gggtcggcac caggccgacc tgcgccagca cctcgcggta
    12961 cagggaccac accgcatcgg caaccggcgc accgtgatcg tccaccagca ggtgcccgcc
    13021 gtcgtggtcc gccaccgttg cgtgcccggc caggtggatc tcgcccaccg cgtgcagcgg
    13081 caatccggcg aggtaggtac gcacgtcgcg gccatggttg cggcagctga cataggcatt
    13141 gttgacgtcc agcagcaggc cgcagccgct gcggcgcacg acctcggcca ggaagccggc
    13201 ctcggcgtgg tcggcggctt cgaattcgag ataggtgctc gggttttcca gcagcatccg
    13261 ccggcccagc aggtcctgca cctcgccgac gtggctgcac acgcggcgca ggccggcgtc
    13321 gtcgtagcga agcggcaaca ggtccgggta gtggacgccg tcgtggctgg accacgccag
    13381 gtgctcggag aactgcgcgg ccggatgccg ctcgagcaac ctgcgcaggc gctgcaggtg
    13441 ggtgcgatcc agcgggtcgt ggccgccgat cgacagcccc acgccatgca ccgacagcgc
    13501 cgtgcgaccg cacagcgccg ccagttggcg atgcggcagg ccgccatcgc cgaaataatt
    13561 ctccgcatgc acctcgacga aggccggcgg tgcgggatcg gccatcagcg catcgaaatg
    13621 ctccggcttg aaaccgatcc cggcctgcat gggcaggccg gggccgggcg cgccggcgcg
    13681 ggggagggtg gcactggcgc gcatcagcgg gctcacatcg ccttggcggg catcgtggct
    13741 tcgaacgccg ccagctggcc cttgccggtc ggcgaggtct tggaggcggt cttggtgcag
    13801 gtgccggcgg caacgtactt ccaggcattc gcctggtggt cgctcttcga ggtgccggcg
    13861 caactggtgc cggggccggc ggcgcagtcg ttcttgccag ccatcgccac gccgtagcac
    13921 ttctccttgg ccatcgcctt gtcgccggcc tgggccgggg tgacggcgcc cagcaaggca
    13981 gaacccagga ccgcggcaag cgcggcggcg gtcagggcgg gggtacgcag atcggacatc
    14041 gggattctcc tgtgggcgca acccgccact cgggttgcac aggaggattc gtcggtgggg
    14101 cgcgtttggt tacacccgcg tgcgaccgcg tgcgcgctgc cggcctacgc gggggtcagg
    14161 ttggcgtagg ccaacaccag ccacttgctg ccggcgtcct cgaagttcac ctgcacccgc
    14221 gcgtgcgcgc cgctgccctc gacatcggtg acgaaaccgg tgccgaaggt gggatggcgc
    14281 acgttcgcgc ccagccgcac tggcgatgcc tcgagcgctg catgcccgcg atcgcgcggg
    14341 gtgaagcgat tcgcctgcat tgcgtaaccc ccgcgcgcga cctgcggctt cgggcggatc
    14401 tcgcgcagca gggccttcgg gatctcgcgc aggaaccgcg acggcaggcc gtacatgtcg
    14461 ctgccgtgga tgcgccgggt ctcggcgtgg ctgagcacca gctgctgccg cgcgcgggtg
    14521 atgccgacgt aggccagccg gcgttcctcg gccatccggc cgggctcttc ggcggagcgc
    14581 atgttcggga acaggccttc ctccaggccg gccaggaaca ccagcgggaa ttccaggccc
    14641 ttggcgctgt gcagggtcat cagctgcacg ccatccgcgc ctgcctgcgc ctgcccttcg
    14701 ccggcttcca gcgaggcata gctgaggaac gccaccagtt cgctcatctg cgcggcgtct
    14761 tcgtcgtcgc cgcggcggaa gcgcgaggcg accgagacca gttcgtccag gttgtcggtg
    14821 cgcgaatcca gctgcccgcg cgactcgttc tcgtaatgca ggcgcaggcc gctgcgcgcg
    14881 agcacgtggt cgatcttttc ctgcagcggc atcgaagcgg tttccaccgc cagcgcatcg
    14941 atcaggcgct cgaacgccgc cagcgcattg cgcgcgcgcg cggcgagcac gctttgcgtg
    15001 cacagcttct gcaccgcgcc ccacagcgag acgccgtgtt cgcgcgcgca caggcgcacc
    15061 tcgtcgaggg tgcgtccgcc gatgccgcgg gtcggcgtgt tgaccgcgcg ctcgaacgcc
    15121 gcgtcgtcgt cgcggttggc gaccagccgc aggtaggcca gggtgtcctt gatctcggcg
    15181 cgctcgaaga agcgcatgcc gccatacacg cgatacggga tctgcgccgc cagcaacgcc
    15241 tcttcgaacg cacgcgactg cgcgttgctg cggtacagga tcgcggcctc gccgtggctg
    15301 ccgccgtcgc gcacccactg ggcgatgcgc tcgatcgcgt aacgcgcttc gtcgacctcg
    15361 ttgtaggcgg cgtacacgtc gatcggctcg ccttcgccgg catcggtcca cagctgcttg
    15421 ccgaggcgat cgtcgttgtg cgcgatcacc gcgttggcgg cgttgaggat gttcgcgctg
    15481 gagcggtagt tctgctcgag cttcagggtg accgcacccg ggtagtcgcg caggaagcgc
    15541 tgcacgttct ccaccttcgc cccgcgccag ccgtagatgg cctggtcgtc gtcgccgacc
    15601 acgaagacgt gcccagaagc cgcctgcccg gtctcgccgg caagcaggcg cacgaagccg
    15661 tactggatcg cgttggtgtc ctggaattcg tcgaccagca actggccgaa gcggtgccgg
    15721 taatgcgcca gcaacgccgg cgtgtcgcgc agcacttcat gcgcgcgcag cagcagttcg
    15781 gcgaaatcga ccagcccggc gcgttcgcag cgcagctggt attcggtgta ggcgcgcagc
    15841 atcacgctgc cccactcgtc cgcttccggc tggatatgct gcggcctgcg cccttcgtcc
    15901 ttctgcgcgt tgatccacca cgcgatctgc cgcggcggga agcgcgcctc gtcgatctcc
    15961 agcgcctgcg ccacgcgctt gaccagccgc tgctggtcgt cggaatccag cacctggaag
    16021 ctttccggca acttcgcgtc ctgccagtgc aggcgcagca gccgatgggc gatgccgtgg
    16081 aaggtgccca cccacatccc gcgcggttcg ccacccagct gctgggcgat gcgcgcgcgc
    16141 atctcgccgg ccgccttgtt ggtgaaggtg accgcgagga tgccgtgcat cggcacgccg
    16201 aacacctcgt gcagccaggc gatgcggtgg gtgagcacgc gggtcttgcc ggaaccggcg
    16261 ccggcgagga cgagatagtg gcccggcgcg gcggacacgg cctcgcgctg ggccgggttc
    16321 agcccgtcga ggaggtggga gacatccatg ccgccattct aacggcgcgc accacccatg
    16381 acctcgacaa gcgagggcgg cgatggccgg gcgttcgcgc agcggcattt cgagccgctt
    16441 ggcgaatgcc cggcgatcgt cgcccgtccg ggcatcagtg cgcggcgcgc ttgcccggcg
    16501 tggccgacat caccttgtcg gtccaggcga tgcccagcgc ggacaggatg aagatggtgt
    16561 ggatgatggt ctgccacatc acgccggtgg cggtgacctt gttgcaggtg accccggcgt
    16621 agccggcggc cagcgcgtcc ttcgcggcag cgaaggccac cggcgtgccg cacagcggca
    16681 tcccatccag ggtgccggcc tcgatgaagg tcttcagcag gtggatcgag gagatgccga
    16741 tgatcgccat cgccagcttg accttgagca cgctggcgtt gacgtggctc agccattccg
    16801 gctggtccgg gtggcgctcc aggcgcatgc gcgagacaaa ggtctcgtag ccacccacga
    16861 tcaccatcat cagcaggttg ctgatcatca ccacgtcgat caggcccagc a
//
LOCUS       DAORUK010000030        16620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_3186_length_16620_cov_19.357501, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000030 DAORUK010000000
VERSION     DAORUK010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 16620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16620
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3186_length_16620_cov_19.357501"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="PK818_03385"
     CDS             complement(<1..877)
                     /locus_tag="PK818_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HQY81809.1"
                     /translation="MSTVRLEAVATEAKREIPMQPASLDIWDKKYRLKTKQGVALDAD
                     IDGTYQRVAKALAEAEPTVELQQYWMERFTWALRRGAIPAGRITSNAGALEHKPATST
                     INCTVSGTIEDSMDGILDKVHEAGLTLKAGCGIGYEFSTLRPRGAFVAGAGAYTSGPM
                     SFMDIYDKMCFTVSSAGGRRGAQMGTFDVSHPDVKDFIRAKREDGRLRQFNLSLLITD
                     GFMDAVNNDQDWPLVFPINIKEQGDINLDDASQVVWREWPTHKNYMTRDDGLVACRIY
                     GHIRARHLWDMIMVST"
     gene            1435..2868
                     /locus_tag="PK818_03390"
     CDS             1435..2868
                     /locus_tag="PK818_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQY81810.1"
                     /translation="MKTATRTLLALTAAAAFGGFAATGMQLMLQRPAAAEPAPAAAAA
                     PAIAALPAAVNGTPMPSLAPMLKRVLPAVVSVNTKQRVRVNTPFGDDPVFRRMFGIPQ
                     ERIAQSLGSGVIVDAARGLVLTNNHVVEDADEVSVTLADGRTLKAEFVGNDADTDIAL
                     MRIPAQELTAIPVANSDRLQIGDFVVAVGNPFGVGQTVTSGIVSAIGRNNLPGAGFQN
                     FIQTDASINPGNSGGALVNLNGELVGINTASFNPRGSMAGNIGLGFAIPSNLASNIKD
                     QLLVGGVVRRGTLGIDTQDVDARIAAALGLAQPRGAVVTRVYPGSAGAAAGLKPGDVI
                     LAANGEPIADAEALRNFQGLQPANARVALDVRRDGQPLLLTTGLREVPKALAGAQLDA
                     RLAGASFADLPESLRRQGLAGVLVEQVERDSRAARSGLRKGDVVLAASSGDFTDLGGF
                     RAGFAAPPPQLVLRVLRGRSSGQLQMR"
     gene            2980..3336
                     /locus_tag="PK818_03395"
     CDS             2980..3336
                     /locus_tag="PK818_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81811.1"
                     /translation="MSTNTDALKSNLGEAGSHLKQAAVAAGDAIKGVAAAAGDEARLG
                     KAQMKSELADGALATLNAAGALGGATGEQVDALMDKGRDLLDSAAELIRERPLAAFGV
                     AFAAGWIISKLGRSDK"
     gene            3346..3855
                     /locus_tag="PK818_03400"
     CDS             3346..3855
                     /locus_tag="PK818_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HQY81812.1"
                     /translation="MQQPHGPAEPVGDAAADAHASPELQEALRALGASGRATLGAASD
                     ATKALRILVAADISLARSAFGRALAFTGVAIAFGASAWLLLMAALIAWLSLGLGLAWS
                     LSLLLSAGLSLAVTAIAAHLAMRYFGHTGLQATRRQLARLGNGELAGLMADAGAPVNT
                     AAGVDVTPP"
     gene            3852..4202
                     /locus_tag="PK818_03405"
     CDS             3852..4202
                     /locus_tag="PK818_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81813.1"
                     /translation="MSFDALIEKVRQAEQALEASERRTLEDWQRFRQAWRSGWTPARI
                     VVAGVVAGFAVGRVQPLRAATGGGVLQVLTALSGLVASGSAQAAAAQAGQAAEAAGTA
                     SGDAAAPFPQPPSG"
     gene            4379..5095
                     /locus_tag="PK818_03410"
     CDS             4379..5095
                     /locus_tag="PK818_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HQY81814.1"
                     /translation="MNFPVRAISLDLDDTLWPFAPIGDRIERTLHDWFVAHSPVTAEH
                     FPIEEMRALRERVFAEFPQHAHDLGLLRRLTIERALRESGSDPTLASAAHAIFFRERN
                     RVDFYPDAPDALRRIAARVPVAALSNGNADLVEIGIAPLFRFQLGAREYGSAKPDPGI
                     FLEACRRLAVAPHEVLHVGDHPEMDVAGAIGAGLRSCWIDRGDHQWPEALPPADLHFT
                     TLTALADWLDDGHPHQEQAA"
     gene            5092..6471
                     /locus_tag="PK818_03415"
     CDS             5092..6471
                     /locus_tag="PK818_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004137823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HQY81815.1"
                     /translation="MSLPTGFATPAQAVAPQPLHVVARDSFAAWRDAQPPAVQAWLAV
                     HRFDGAAGTALAWADAQGQLAGAVIGIGDALDPHAYAHAPTALPGADWQLAGSPGAGA
                     RCALQLGWGLGCYRFDRYKASTRAPARLVADDFDAETFDLLAACASVRDLVNTPTEHM
                     GPDELEAAARALAQAHGAAVEVVAGDDLLAQNYPAIHAVGRASHRAPRIIELAWGDAA
                     HPHVAICGKGVCFDTGGLDIKAATGMRNMKKDMGGAAHALALAGLVMARKLPLRITLL
                     VAAVENAIGPNAFRPGEVIATRKGVSVEIDNTDAEGRIVLCDALARAGELKPDLLLDF
                     ATLTGAARVALGPDLPALYANDEALAADWLTAGQQVRDPLWRMPLWRPYLRYLTSSVA
                     DLANGSATTMAGSVTAALFLERFVAEGQPWGHVDVYAWNDSDRPGKPAGGEAQGLRAA
                     YALLKSRTT"
     gene            6566..7153
                     /locus_tag="PK818_03420"
     CDS             6566..7153
                     /locus_tag="PK818_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81816.1"
                     /translation="MTKTEQLQQDLDYITATVRRREQPVGIPALYLFWATAVLVGFAS
                     ADFAPRITGLFWFVVGIGGGLLSWWMGQRDDRRRGIRDEALGRRHGLHWVLGGVAYFL
                     TALPMFVHGASNDRAASFLLTTGIVYGLAGVHLERPLLWCGLLAFGGYAALVALSLPY
                     AWTLSGVLIAACLVLAAVFAARVRAAEQIGREQAQ"
     gene            7150..7449
                     /locus_tag="PK818_03425"
     CDS             7150..7449
                     /locus_tag="PK818_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQY81817.1"
                     /translation="MKALDGLDPAFEHRARLAIAVLLARHGEISFARFKQELALTDGN
                     LGAQLRRLEDDGYLALRRDFVERRPVTWYALSKAGRKALDTHLSALRAVIAASAG"
     gene            7535..8785
                     /locus_tag="PK818_03430"
     CDS             7535..8785
                     /locus_tag="PK818_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY81818.1"
                     /translation="MSSNADLLNALKIDRNAPPPSSRKGLWIALAAVAAVLLAVLGWF
                     VFGRDTGIAVRTAEAMAIGNGAGGSASVLDATGYVVARRMATVSAKIPGKVREVLIEE
                     GQRVEEGQVIATLDPIDAEQQRMLSASQLAAAQSQAAGVQAQLKEAEANAARLAPLAA
                     QKLVSRAQLEQAIAQRDSLRAQVATAQRNAQVAGDALRISAQGVDNTIVRAPFAGVVI
                     AKAAQPGEIISPMAASGGFTRSGIGTIVDMESLEVEVEVGEAFIGRVKPGMPTEAVLN
                     AYQDWKIPGRVIAIIPAADRGKATVKVRVGLDARDPRIVPDMGVRVGFLEDAKPADAA
                     AKPGVLVPAAAITQRDGNDVAFVLAGDAVQQRTLQLGRTLGEDREVLAGLAGGDAVVL
                     DPPEQLADGARVRLADATAGADGR"
     gene            8850..9542
                     /locus_tag="PK818_03435"
     CDS             8850..9542
                     /locus_tag="PK818_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY81819.1"
                     /translation="MPALVSIRNLNKTYQRGPEKVDVLHGIDLDIEQGDFVALMGPSG
                     SGKTTLLNLIGGLDSPTAGQIEVAGQRIDTLKSGQLSQWRSNHVGFVFQFYNLMPTLT
                     AQRNVELPLLLTPLSSAQRKRNAEIALQLVGLADRGKHRPAELSGGQQQRVAIARAIV
                     SDPTLLICDEPTGDLDRASAEEILRLLQTLNREHGKTIVMVTHDPKAAEYATHTVHLD
                     KGNLVEQAGHAA"
     gene            9555..10712
                     /locus_tag="PK818_03440"
     CDS             9555..10712
                     /locus_tag="PK818_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010374852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY81820.1"
                     /translation="MKYLHLVWAALFRSKTRTLLTLLSVVAAFLLFGMLDSVRVAFNS
                     GGSVAGANRMVTMSRLSITQMLPYSLDAQIRALPGVKRAAYAAWFGGIYRDPKNFFAN
                     FSVSPDYLDLYPEFKLPPAQKQAWLADRRGAIVGESLATRFGWKIGDTIPLQATIFPT
                     KGSNDWPFTLRGIYVVEDQKMKGQENVMFFHWTYFDEANDYVKGRVGWWIVEPASADA
                     ADRVARAVDTLSENSDHETRTQSEAAFNQSFAKQFADIGLIVTAIMGAVFFTLLLLTG
                     NTMAQAVRERIPELAVLKTIGFSNRSVLWLVLGESVLLVVLGGAIGLGFAALLVPVVS
                     AGSGGMIQLPGLLPQTWAMGLGLAALIGVVVGLLPALRGMRLNIVDALAGR"
     gene            10740..12056
                     /locus_tag="PK818_03445"
     CDS             10740..12056
                     /locus_tag="PK818_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY81821.1"
                     /translation="MTRFREFLAGLLTLVLLAVGLGIWIRLPWPAVLAITLGLALWLF
                     LTRSGRLAREATRIGIAGLPQRWGASLVIVVGIAGVVGVLVAMLAMGEGFKATLASTG
                     STGSAILLRAGSQAETNSVIRRDQVPLLATLPGIARGTDGRPLASPELSQVVNLPTMA
                     EGTDANVQFRGVGPAGWMIRPQLEIVQGRRFTPGMREIVVGTGTHQQFRGLEVGNQLK
                     LANQLWTVVGRFKSGDSHDSELWADADVLGPAYQRSAFQSVTVKLDGKDGLARLKAAL
                     AADPRLKLDIETTRDYYGRQSEGLTRLIRILGTVIGGIMAIGAVFGALNTMYAAVAGR
                     AREIATLRALGFRGLPVVAAVMLETMLLALLGGLLGAALAWLLFNGYTVSTLGQNFSQ
                     VVFQFRVSPELLWTGLKWALGIGLVGGLFPALRAARVPVSEALRAA"
     gene            complement(12064..12975)
                     /gene="rarD"
                     /locus_tag="PK818_03450"
     CDS             complement(12064..12975)
                     /gene="rarD"
                     /locus_tag="PK818_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007966951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HQY81822.1"
                     /translation="MSDAARREAIRGTWIAAATFVLWGVMPLYWHLLKAVPSLQIVLH
                     RIAWSALFVGAFLLWRDGHGWLRAALAQPRLRAMLALSGLLIAFNWGLYIWAVNAGHV
                     VESALGYFINPLLNVLIGVLFLRERLRPLQWLSVAIAAVGVLWLTFRFGRLPWIALAL
                     AASFALYGLIRKLLAVDSITGLGVESAYLLLPAVALLAWSEAPASGGFPAGYGAAASS
                     LLVLSGIVTALPLVGFAYAVRRIPLASVGLMQYIAPTLQFLIGVLVLREDFDQARLFG
                     FVLIWIALAVFAGEGLLRARRGRPATA"
     gene            complement(12972..13865)
                     /gene="yedA"
                     /locus_tag="PK818_03455"
     CDS             complement(12972..13865)
                     /gene="yedA"
                     /locus_tag="PK818_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HQY81823.1"
                     /translation="MDKAHTVAGTRVALALAAVYIIWGSTYLAIRYALEGGFPPFLLG
                     GVRFLIAGSLMYGFLRWRGVPAPTAPQWRNAAVMGVLLLLLGNGMVNLAEQTVSSGMT
                     AVAVASAPLWMGIFAAMRGQRPNRMEWVGLGIGFLGVLWLNLDSSLSASMVGLVALLV
                     ASLAWSFGSIWSRGRDLPPPFMAAAAQMLCGGLAMCVVGGALGERFAGIPGPQGLAAF
                     AYLIVFGSIIGFSAYIWLLHHVRPALAGSYAYVNPVIAVALGAWLARERFGGQELLAM
                     GVILLGVVAITLAKAAKARPA"
     gene            complement(13959..16151)
                     /locus_tag="PK818_03460"
     CDS             complement(13959..16151)
                     /locus_tag="PK818_03460"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HQY81824.1"
                     /translation="MKSSHTLLALAVGLALAAPLALLARAEGSRDALPAAPTSNQATT
                     ARLVHGLLSDSRYAYRPRVLDDALSQDVLERYLDTLDPGKVFLTAQDVAGFSKFATRL
                     DDAIKSGEVDPAWAMFAVYRQRVGERIAHARGLLKGNFDFAGGERYEYDREDAPWAAS
                     GAELDALWKQSVKNDWLRLKLAGKPPEEIRKTLDKRYANLLDSIAELKGEDVFQSFLN
                     AYAGAIDPHTDYMTPRSAANFNVQISNSLEGIGAVLFKQDDIVVVREMVPGGPAARGG
                     KLRSGDRIVGVGQGGSGEMKDVVGWPTDDVVDLIRGAANTQVRLDVVSAEAPLDSKPT
                     RILITRAKVRLEDARAKAEEVLLPAAGGQPARRIGVIKLPGFYQDVEARRRRDGDYAS
                     ATRDVARMLEQFRAQKVDGVVLDLRGNGGGSLSEAIELTGLFIDQGPVVQVRESGGRV
                     SVENDRNPGVAWDGPLAVLVNRGSASASEIVAGAIQDYGRGLVIGETTFGKGTVQSMV
                     DLDRWPANEELRFGQVKLTIAQFFRPGGSSTQNRGVVPDVAFPTSVDASEYGESTYDN
                     ALPWTRIAAAPHVQYGNFGSLLGKLEARHAARVANDVEFQWWVEDVARFREEQAKKSV
                     SLNETERRAERDRFDRLRKQRAAVRANLGIAQDPLLEQQAADDGLQANERNVAADVAR
                     EEAAKKSRDPLLRESAAILGDAIGLLGADAGLSAQVLPQARGGTRWVD"
     gene            complement(16369..>16620)
                     /locus_tag="PK818_03465"
     CDS             complement(16369..>16620)
                     /locus_tag="PK818_03465"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004090539.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HQY81825.1"
                     /translation="GLGETMEQVQATLRDLRAHDVEMVTIGQYLQPSPAHHPLLRYWT
                     PDEFKALEDYGYSIGFSHVASGPMVRSSYHADRQAAGLA"
BASE COUNT         2444 a         6015 c         5798 g         2363 t
ORIGIN      
        1 acgtcgagac catgatcatg tcccacaggt ggcgggcgcg gatgtggccg tagatgcggc
       61 aggcgaccag gccatcgtcg cgggtcatgt agttcttgtg ggtgggccac tcgcgccaga
      121 ccacctggga ggcgtcgtcg aggttgatgt cgccctgttc cttgatgttg atcgggaaca
      181 ccagcggcca gtcctggtcg ttgttcaccg cgtccatgaa gccgtcggtg atcagcagcg
      241 acaggttgaa ctggcgcagg cggccgtcct cgcgcttggc gcggatgaag tccttcacgt
      301 ccgggtggct gacgtcgaag gtgcccatct gcgcgccgcg gcggccaccg gccgaactca
      361 cggtgaagca catcttgtcg tagatatcca tgaacgacat cgggccgctg gtgtacgcgc
      421 cggcgccggc gacgaacgcc ccgcgcgggc gcagggtgga aaactcgtag ccgatcccgc
      481 agcccgcctt cagggtcagg ccggcttcgt ggaccttgtc gaggatgccg tccatcgaat
      541 cctcgatggt gccggacacg gtgcagttga tggtgctggt ggcgggcttg tgttccagcg
      601 cgccggcgtt gctggtgatg cggccggcgg gaatcgcccc gcggcgcagc gcccaggtga
      661 agcgctccat ccagtactgc tgcagctcga cggtgggctc ggcctcggcc agcgccttgg
      721 cgacgcgctg gtaggtgccg tcgatgtcgg catccagggc cacgccctgc ttggtcttca
      781 ggcggtactt cttgtcccag atgtccagtg aagccggctg catcgggatc tcccgcttcg
      841 cttcagtcgc caccgcttcc agcctcaccg tgctcatgcc cgccatagct catcctgctc
      901 ctgtccgtgt cgacccgcgg cgcgccgcgt ccgtgttgtt cgccatgccg tcgcgggggc
      961 gacgacgaag cctcgcgccg ttcgcgcgcg acccgttcca ttcgatttcc gctgcatcgt
     1021 ccatctgcgc gccgctcccc aacggacgcc gatccctgca ccccgtgaaa cacaagaatt
     1081 gggcttgttc cacggtgcca accacaagat gtagtgggcg cggaaacgga ccttatccag
     1141 ccgtcgcggc gaagtcaacg ccagaaatcg gcgaaatccg cttgcaatcc ggacgcgatc
     1201 tggcacacgc gcatcggcac tgggtttcgc cgcgacggct gcggttgccg ccaattatgt
     1261 gaatgcgctc acacccgcat ccggggatcg cggcttgcgc accgcaacat ggcggcgccg
     1321 gaattgccgc acccgcgcat ggccccgcgg ccggccacgc ttgccaccct tcagcggaga
     1381 ttgcacgatc gcgatcgaaa ctgaatggac ttgcgttcca gccatgcgaa gccgatgaag
     1441 accgccacca ggaccctgct cgccctcacc gccgccgccg cgttcggtgg cttcgccgcc
     1501 accggcatgc agctgatgct gcaacggccg gccgccgccg aaccggcgcc cgcggcggcc
     1561 gccgcgcccg cgatcgcggc gctgccggcg gcggtcaacg gcaccccgat gccttcgctg
     1621 gcaccgatgc tcaagcgggt gctgccggcg gtggtcagcg tcaacaccaa gcagcgggtg
     1681 cgggtgaaca ccccgttcgg ggacgacccg gtgttccgcc gcatgttcgg catcccgcag
     1741 gaacggatcg cgcagtcgct gggctcgggg gtgatcgtgg acgcggcgcg cgggctggtg
     1801 ctgaccaaca accacgtggt cgaggatgcc gacgaagtct cggtgacgct ggccgatggc
     1861 cgcaccctca aggccgagtt cgtcggcaac gatgccgaca ccgacatcgc cctgatgcgg
     1921 atcccggcgc aggagctgac cgcgatcccg gtggccaaca gcgaccgcct gcagatcggc
     1981 gacttcgtgg tcgcggtcgg caacccgttc ggggtcgggc agacggtgac ctccggcatc
     2041 gtttccgcga tcggccgcaa caacctgccg ggtgcgggct tccagaactt catccagacc
     2101 gatgcctcga tcaaccccgg caactcgggc ggcgcgctgg tcaacctcaa tggcgagctg
     2161 gtcgggatca acaccgccag tttcaacccg cgcggctcga tggccggcaa catcggcctg
     2221 ggcttcgcga tcccgtccaa cctggccagc aacatcaagg accagctgct ggtcggcggc
     2281 gtggtccggc gcggcaccct cggcatcgac acccaggacg tggacgcgcg catcgccgcc
     2341 gcgctgggac tggcgcagcc gcgcggcgcg gtggtgaccc gggtgtatcc gggctccgcc
     2401 ggcgcagccg ccgggctcaa gccgggcgac gtgatcctgg ccgccaacgg cgagccgatc
     2461 gccgacgccg aagccctgcg caacttccag ggcctgcagc cggcgaacgc gcgggtcgcg
     2521 ctggacgtgc gccgcgatgg ccagccgctg ctgctcacca ccggcctgcg cgaagtgccc
     2581 aaggcgctgg ccggcgccca gctggacgcg cggctggcgg gcgcgagctt cgccgacctg
     2641 ccggagtcgc tgcgccggca gggcctggcc ggggtgctgg tcgagcaggt cgagcgcgac
     2701 agccgcgcgg cccgcagcgg cctgcgcaag ggcgacgtgg tgctggccgc gagcagcggc
     2761 gacttcaccg acctcggcgg cttccgcgcc gggttcgccg cgccgccgcc gcaactggtc
     2821 ctgcgggtgc tgcgcggccg cagcagtggc cagttgcaga tgcgttgaac gttgcttgca
     2881 ggacagccct cgccgccacg ccatgacggg cgcgtcacgc gcgggctggc acactcccgg
     2941 cgtgcgtccc accccaccca ccgttcccga ggatccgcga tgagcaccaa taccgatgcg
     3001 ttgaagagca acctgggcga agccggctcc cacctcaagc aggccgcggt tgccgccggc
     3061 gacgcgatca agggcgtggc ggcagccgcc ggcgacgagg cgcggctggg caaggcgcag
     3121 atgaagtcgg aactggcgga tggcgcgctc gccaccctga atgccgccgg cgcactcggt
     3181 ggcgccaccg gcgagcaggt cgacgcgctg atggacaagg gccgcgacct gctcgacagc
     3241 gccgccgagc tgatccgcga gcgcccgctg gccgccttcg gggttgcctt cgccgccggc
     3301 tggatcattt ccaagctcgg ccgcagcgac aagtaagccg cgcacatgca gcagccgcat
     3361 ggcccggcgg aacccgtcgg cgacgccgcc gcggacgcgc acgccagccc ggagttgcag
     3421 gaggcgctgc gcgcgctggg cgcgagcggc cgcgccacac tcggcgccgc cagtgatgcc
     3481 accaaggcgc tgcggatcct ggtcgccgcc gacatctcgc tggcgcgcag cgcgttcggg
     3541 cgcgccctgg ccttcaccgg ggtcgcgatc gccttcggcg cgtccgcgtg gttgctgctg
     3601 atggcggcgc tgatcgcgtg gctgagcctg ggcctgggcc tggcgtggtc gttgtcgctg
     3661 ctgctcagcg ccgggctgag cctggcggtg accgccatcg cggcccacct ggcgatgcgc
     3721 tatttcggac acaccgggct gcaggcgacc cgccggcagc tggcgcggct ggggaacggc
     3781 gaactcgccg gtttgatggc ggacgccggc gccccggtca acacggctgc gggcgtggac
     3841 gtgaccccgc catgagtttc gatgcactga tcgagaaggt ccgccaggcc gagcaggcgc
     3901 tggaagccag cgagcgccgc accctcgagg actggcagcg cttccgccag gcctggcggt
     3961 ccggctggac cccggcgcgc atcgtggtcg ccggggtggt cgccggtttc gcggttggcc
     4021 gcgtgcagcc gctgcgcgcg gccaccggcg gcggcgtgct gcaagtgctg accgcgctgt
     4081 ccgggctggt ggcaagcggc agcgcgcagg ccgccgccgc gcaggcaggg caggcggcgg
     4141 aagccgccgg aaccgcctcc ggcgacgcgg ccgccccctt cccgcaaccg ccgtccggat
     4201 gaacgcgcgc gcagcgccgg cgccagcccc gacctgacct ggcccgcacg ccccggcgca
     4261 ggtcgccgcc gcgggtctgc gccagcatcg tgctggcccg gatcgaccgg ttcgagggcc
     4321 gggcgacgct gctcgaatga caggcgcagc gggacaagcc ccgctctaca atgggccggt
     4381 gaacttcccc gtccgcgcca tcagcctcga cctcgacgac accctttggc cgttcgcgcc
     4441 gatcggcgac cgcatcgagc gcaccctgca cgactggttc gtggcgcaca gcccggtgac
     4501 cgccgagcac ttcccgatcg aagagatgcg cgccctgcgc gaacgcgtgt tcgccgagtt
     4561 cccgcagcac gcgcacgacc tcggcctgct gcggcggctg accatcgaac gcgcgctgcg
     4621 cgagagcggc agcgacccga ccctggccag tgccgcgcat gcgatcttct tccgcgaacg
     4681 caaccgcgtg gacttctacc cggatgcgcc ggacgcgctg cgccggatcg ccgcgcgggt
     4741 gccggtggca gcactgtcca acggcaatgc cgacctggtg gagatcggca tcgcgccgct
     4801 gttccggttc cagctcggcg cgcgcgaata cggttccgcc aagcccgatc ccgggatctt
     4861 cctggaagcc tgccgccggc tggctgtcgc cccgcacgaa gtcctgcacg tcggcgacca
     4921 tccggagatg gacgtggccg gcgcgatcgg cgccggcctg cgcagctgct ggatcgaccg
     4981 cggcgaccac caatggcccg aggcgttgcc gccggccgac ctgcacttca ccaccctgac
     5041 cgcgctggcc gactggctgg acgacggcca cccccaccag gaacaggccg catgagcttg
     5101 ccgaccggtt tcgcgacgcc cgcacaggcc gtcgcgccgc agcccctgca cgtggtcgcg
     5161 cgcgacagct tcgccgcttg gcgcgatgcg cagccacccg cagtgcaggc atggctggcg
     5221 gtgcaccgct tcgacggcgc cgccggcacc gcgctggcgt gggcggatgc gcagggccag
     5281 cttgccggcg cggtgatcgg gatcggcgac gcgctggatc cccatgccta tgcgcatgca
     5341 ccgaccgcct tgccgggcgc cgactggcag ctcgccggca gccctggcgc cggcgcgcgc
     5401 tgcgcgctgc aactcggctg gggcctgggc tgctaccgct tcgaccgcta caaggccagc
     5461 acccgcgcgc cggcgcggct ggtggcggac gacttcgacg ccgagacctt cgacctgctg
     5521 gccgcctgcg cgagcgtgcg cgacctggtc aacacgccca ccgagcacat gggcccggac
     5581 gagctggaag cggccgcgcg cgcactggcg caggcgcacg gcgcggcggt cgaggtggtc
     5641 gccggcgacg acctgctggc gcagaactac ccggcgatcc acgcggtcgg ccgcgcctcg
     5701 caccgggcgc cgcggatcat cgaactggcg tggggcgacg ccgcgcatcc gcatgtcgcc
     5761 atctgcggca agggcgtgtg cttcgacacc ggcgggctgg acatcaaggc cgccaccggc
     5821 atgcgcaaca tgaagaagga catgggcggc gccgcgcatg cgctggcgct ggccgggctg
     5881 gtgatggcac gcaaactgcc gttgcggatc accctgctgg tcgccgcggt ggagaacgcg
     5941 atcgggccga acgcgttccg gcccggcgag gtgatcgcca cccgcaaggg cgtcagcgtg
     6001 gagatcgaca acaccgatgc cgaaggccgc atcgtgctgt gcgacgcact ggcgcgtgcg
     6061 ggcgaactca agcccgacct gctgctggac ttcgccaccc tcaccggcgc ggcgcgggtt
     6121 gcgctgggcc cggacctgcc ggcgctgtac gcgaatgacg aggcactggc ggccgactgg
     6181 ctcaccgccg gccagcaggt gcgcgacccg ctgtggcgga tgccgctgtg gcggccatac
     6241 ctgcgctacc tcaccagcag cgtggccgac ctcgccaacg gcagcgccac caccatggcc
     6301 ggcagcgtca ccgccgccct gttcctcgaa cgcttcgtcg ccgaaggcca gccctggggc
     6361 cacgtcgacg tctacgcctg gaacgacagc gaccgccccg gcaagcccgc cggcggcgag
     6421 gcgcagggcc tgcgcgcggc ttacgccctg ctgaaatccc gcaccacctg aagcgaccgt
     6481 tcgtcggctg ggctacttgc gcacttgcgt actctgtttt ttagagtact ctgcatatca
     6541 ccaatcccaa ccaatgggca ccaccgtgac caagaccgaa cagttgcagc aggacctgga
     6601 ctacatcacc gccaccgtgc gccggcgcga acagccggta ggcattcccg ccctctacct
     6661 gttctgggcc acggcggtgc tggtgggctt cgcctccgcc gacttcgccc cgcgcatcac
     6721 cggcctgttc tggttcgtgg tcggcatcgg cggcggcctg ctcagctggt ggatgggcca
     6781 gcgcgacgac cgccggcgcg gcatccgcga cgaagccctg ggccggcgcc atggcctgca
     6841 ctgggtgctc ggcggcgtgg cctacttcct caccgcactg ccgatgttcg tgcacggcgc
     6901 cagcaacgat cgcgcggcga gcttcctgct caccaccggc atcgtgtacg gcctggccgg
     6961 cgtgcacctg gagcgcccgc tgctgtggtg cggcctgctc gccttcggcg gctatgccgc
     7021 gctggtggcg ttgtcgctgc cgtatgcctg gaccctctcc ggggtgctga tcgcagcctg
     7081 cctggtgctg gccgccgtgt tcgccgcgcg cgttcgcgcc gccgaacaga tcgggcggga
     7141 gcaggcgcaa tgaaagcgct cgacggcctc gaccctgcgt tcgagcaccg cgcgcggctg
     7201 gcgatcgcgg tgctgctggc ccgccacggc gagatcagct tcgcccgctt caagcaggaa
     7261 cttgcgctca ccgatggcaa cctcggcgcg cagctgcggc ggctggagga cgacggctac
     7321 ctcgccctgc gccgcgactt cgtcgaacgc aggccggtga cctggtatgc gttgagcaag
     7381 gccgggcgca aggcgctgga cacccacctt tccgcgctgc gggcggtgat cgcggcgtcc
     7441 gccgggtgac ccaagaaact gacttccggc aggttggcgc cagcttgcgc actcggtcac
     7501 acttgcgcgc cgctcgccgt ccccggattg ctcgatgagt tccaatgccg acctgttgaa
     7561 cgcgttgaag atcgatcgca acgcgccgcc gcccagctcg cgcaagggac tgtggatcgc
     7621 gctggccgcg gtcgccgcgg tgctgctggc ggtgcttggc tggttcgtgt tcggccgcga
     7681 caccggcatc gcggtgcgca ccgcggaagc gatggcgatc ggcaatggcg ccggcggcag
     7741 tgcctcggtg ctggacgcca ccggctacgt ggtcgcccgg cgcatggcca cggtctcggc
     7801 gaagatcccc ggcaaggtcc gcgaggtact gatcgaggaa ggccagcggg tggaggaagg
     7861 ccaggtgatc gccaccctcg acccgatcga cgccgagcag cagcgcatgc tctcggcctc
     7921 gcaactggcc gccgcgcaga gccaggccgc cggcgtgcag gcgcaactga aggaggcaga
     7981 ggccaatgcc gcgcgccttg ccccgctggc cgcgcagaag ctggtgtcgc gcgcgcaact
     8041 cgagcaggcg attgcccagc gcgattcgct gcgcgcgcaa gtggccaccg cgcagcgcaa
     8101 cgcgcaggtc gccggcgacg cgctgcgcat ttccgcgcaa ggagtggaca acaccatcgt
     8161 gcgcgcgccg ttcgccgggg tggtgatcgc caaggcggcg cagccgggcg agatcatctc
     8221 gccgatggcg gccagtggcg gcttcacccg ctccggcatc ggcaccatcg tcgacatgga
     8281 gtcgctggag gtcgaagtgg aggtcggcga ggccttcatc ggccgggtca agcccggcat
     8341 gccgaccgaa gcggtgctca atgcctacca ggactggaag atccccggcc gggtgatcgc
     8401 gatcatcccc gccgccgacc gcggcaaggc cacggtcaag gtgcgggtgg ggctcgacgc
     8461 cagggacccg cggatcgtgc ccgacatggg cgtgcgggtg ggcttcctgg aggacgccaa
     8521 gccggcggac gccgccgcga agccgggcgt gctggtgccg gccgcggcga tcacgcagcg
     8581 tgatggcaac gatgtggcct tcgtgctcgc cggcgacgcc gtgcagcagc gcacgctgca
     8641 actgggccgc accctcggcg aagaccgcga ggtgctcgcg ggcctggccg gcggcgacgc
     8701 agtggtgctg gacccgcccg aacaactcgc cgacggcgcg cgcgtgcgcc ttgccgacgc
     8761 gaccgcaggc gccgacgggc gctgacgtac cccatccccc gcccgcccgc ggccaccgcc
     8821 gcggccccgg caccaccaca ggatccgcca tgcctgcgct cgtcagcatc cgcaacctca
     8881 acaagaccta ccagcgcggc ccggaaaagg tcgacgtgct gcatggcatc gacctcgaca
     8941 tcgaacaggg cgatttcgtc gcgctgatgg gtccctccgg ctccggcaag accaccctgc
     9001 tcaacctgat cggcgggctg gactcgccga ccgcggggca gatcgaggtg gccggccagc
     9061 gcatcgacac cctgaagtcc ggccagctct cgcagtggcg cagcaaccac gtcggcttcg
     9121 tgttccagtt ctacaacctg atgccgaccc tcaccgcgca gcgcaacgtc gagctgccgt
     9181 tgctgctgac cccgctgtcg tcggcgcaac gcaagcgcaa tgcggagatc gcgctgcaac
     9241 tcgtgggcct ggccgaccgc ggcaagcacc gcccggcgga actgtccggc ggccagcagc
     9301 agcgcgtggc gatcgcccgc gcgatcgtct ccgacccgac cctgctgatc tgcgacgaac
     9361 ccaccggcga cctcgaccgc gcctccgccg aggagatcct gcgcctgctg cagaccctca
     9421 accgcgagca cggcaagacc atcgtgatgg tcacccacga ccccaaggcc gcggagtacg
     9481 ccacccacac cgtgcacctg gacaagggca acctggtcga gcaggccggg catgcggcct
     9541 gacggagcac gccgatgaaa tacctccacc tggtctgggc cgcgctgttc cgcagcaaga
     9601 cccgcaccct gctgaccctg ctgtcggtgg tcgccgcgtt cctgctgttc gggatgctcg
     9661 attcggtgcg ggtcgccttc aattccggcg gcagcgtggc cggcgccaac cggatggtga
     9721 cgatgtcgcg gctgtcgatc acgcagatgc tgccgtacag cctggatgcg cagatccgcg
     9781 cgctgccggg ggtgaagcgc gcagcctacg cggcgtggtt cggcggcatc taccgcgacc
     9841 cgaagaactt cttcgccaat ttcagcgtca gccccgacta cctggacctg taccccgagt
     9901 tcaagttgcc gccggcgcag aagcaggcgt ggctggcgga ccgccgcggc gcgatcgtcg
     9961 gcgagtcgct ggcaacgcgc ttcggctgga agatcggcga caccatcccg ctgcaggcca
    10021 cgatcttccc gaccaagggc agcaacgact ggccgttcac cctgcgcggc atctacgtgg
    10081 tcgaggacca gaagatgaag ggccaggaga acgtgatgtt cttccactgg acgtacttcg
    10141 acgaagccaa cgactacgtc aagggtcggg tcggctggtg gatcgtggag ccggccagcg
    10201 ccgatgccgc cgaccgcgtg gccagggccg tcgacacgct cagcgagaac tccgaccacg
    10261 aaacccggac ccagagcgag gccgcgttca accagagctt cgccaagcag ttcgccgaca
    10321 tcggcctgat cgtcaccgcg atcatgggcg cggtgttctt caccctgctg ctgctgaccg
    10381 gcaacaccat ggcgcaggcg gtgcgcgagc gcatccccga actggcggtg ctgaagacca
    10441 tcggcttctc caaccgcagc gtgctgtggc tggtgctggg cgagtcggtg ctgctggtgg
    10501 tgctgggcgg cgcgatcggg ctgggcttcg ccgcgctgct ggtgccggtg gtcagtgccg
    10561 gcagcggcgg catgatccag ctgcccggcc tgctgccgca gacctgggcg atgggcctgg
    10621 gcctggccgc gctgatcggg gtggtggtgg gcctgctgcc ggcgttgcgc ggcatgcggc
    10681 tcaacatcgt cgacgcactg gccggtcgtt gagcaagcgt tgagcaagga caccaacaca
    10741 tgacgcgttt cagggaattc ctggccgggc tgctgaccct ggtcctgctg gccgtgggac
    10801 tgggtatctg gatccggctg ccgtggcccg cggtgctcgc catcaccctt gggcttgcgc
    10861 tgtggctgtt cctcacccgc agcggccggc tggcgcgcga agccacccgg atcggcatcg
    10921 ccggcctgcc gcagcgctgg ggggcgtcgc tggtgatcgt ggtcggcatc gccggggtgg
    10981 tcggggtgct ggtggcgatg ctggcgatgg gcgaaggctt caaggccacc ctggccagca
    11041 ccggcagcac cggcagcgcg atcctgctgc gcgccggttc gcaggcggaa accaattcgg
    11101 tgatccgccg cgaccaggtg ccgctgctgg cgaccctgcc cggcatcgcc cgcggcaccg
    11161 acggcaggcc gctggcgtcg ccggagttgt cgcaggtggt caacctgccg accatggccg
    11221 aaggcaccga cgccaatgtc cagttccgcg gcgtcggccc cgccggctgg atgatccgcc
    11281 cgcagctgga gatcgtgcag ggccgcaggt tcacgcccgg catgcgcgag atcgtggtgg
    11341 gtaccggcac ccaccagcag ttccgcgggc tggaggtcgg caaccagctg aaactggcca
    11401 accagctgtg gacggtggtc gggaggttca agtccggcga ctcccacgat tccgaactgt
    11461 gggccgacgc cgacgtgctg ggcccggcct accagcgcag cgcattccag tcggtgacgg
    11521 tgaagctgga tggcaaggac ggccttgcgc ggctgaaggc ggcgctggcc gccgatccgc
    11581 ggctgaagct cgacatcgag accacccgcg actactacgg ccggcaatcc gagggcctga
    11641 cccggctgat ccggatcctg ggcacggtga tcggcggcat catggcgatc ggcgcggtgt
    11701 tcggcgcgct caacaccatg tacgccgccg tcgccggccg cgcccgcgag atcgcgaccc
    11761 tgcgcgcgct cggcttccgc gggctgccgg tggtcgccgc ggtgatgctg gaaaccatgc
    11821 tgctggccct gctcggcggg ctgctcggtg ccgcgctggc gtggctgctg ttcaacggct
    11881 acacggtcag cacgctgggc cagaacttca gccaggtggt gttccagttc cgggtctcgc
    11941 cggagctgct gtggaccggc ctgaagtggg cactcggcat cggcctggtc ggtggcctgt
    12001 tcccggccct gcgcgccgcg cgggtgccgg tgagcgaggc cctgcgcgcc gcctgatcga
    12061 ggctcaggcg gtggcgggcc ggccgcgccg cgcgcgcagc aggccttcgc cggcgaacac
    12121 cgccagcgcg atccagatca gcacgaaacc gaacagccgg gcctggtcga agtcctcgcg
    12181 caggaccagc acgccgatca ggaactgcag ggtcggcgcg atgtactgca tcaggccaac
    12241 actggccagc gggatgcgcc gcaccgcgta ggcgaagccg accagcggca acgcggtgac
    12301 gatcccggac aacaccagca ggctgcttgc cgcggcgccg tagccggccg ggaagccgcc
    12361 gctggccggc gcctcgctcc acgccagcag ggccaccgcc ggcagcagca ggtaggcgct
    12421 ctccaccccc agcccggtga tggaatccac cgccagcagc ttgcggatca acccgtacag
    12481 cgcgaacgag gcggccagcg ccagcgcgat ccacggcagc cggccgaacc ggaaggtcag
    12541 ccacaacacg ccaaccgccg cgatcgcaac cgacagccac tgcagcgggc gcagccgctc
    12601 gcgcaggaac aacaccccga tcagcacgtt gagcagcggg ttgatgaagt acccgagcgc
    12661 gctctcgacc acgtggccgg cgttcaccgc ccagatgtac aggccccagt tgaacgcgat
    12721 cagcagcccg ctcagggcca gcatcgcgcg cagccgcggt tgcgccagcg ccgcgcgcag
    12781 ccagccgtgg ccgtcgcgcc acagcaggaa ggcgccgacg aacagcgcgc tccaggcgat
    12841 ccggtgcagc acgatctgca gcgacggcac cgccttcagc aggtgccagt acagcggcat
    12901 caccccccac agcacgaagg tcgccgcggc gatccacgtg ccgcggatcg cctcgcgccg
    12961 cgcggcgtcg ctcatgccgg ccgggccttc gcggccttgg ccagggtgat cgcgaccacc
    13021 cccagcagga tcacgcccat cgccagcagc tcctgcccgc cgaagcgctc gcgcgccagc
    13081 cacgcgccca gcgccaccgc gatcaccggg ttgacgtagg catagctgcc ggccagcgcc
    13141 gggcgcacgt ggtgcagcag ccagatgtag gcggagaagc cgatgatcga accgaacacg
    13201 atcaggtagg cgaacgcggc caggccctgg gggcccggga ttccggcgaa gcgctcgccg
    13261 agcgcgccgc cgaccacgca catcgccagc ccgccgcaca gcatctgcgc ggcggcggcc
    13321 atgaacggcg gcggcaggtc gcggccacgg ctccagatcg aaccgaacga ccacgccagc
    13381 gaggcaacca gcaacgcgac caggcccacc atcgacgccg acaggctgct gtcgaggttc
    13441 agccacagca cgccaaggaa gccgatgccg aggcccaccc actccatccg gttcggccgc
    13501 tgcccgcgca tcgccgcgaa gatgcccatc cacaacggcg ccgacgcgac cgccaccgcg
    13561 gtcatcccgg aggagacggt ctgctccgcc aggttgacca tgccgttgcc cagcaacagc
    13621 agcagcaccc ccatcaccgc cgcgttgcgc cattgcggag cggtcggcgc gggcaccccg
    13681 cgccagcgca ggaacccgta catcaggctg ccggcgatca ggaagcgcac gcccccgagc
    13741 aggaacggcg ggaagccgcc ttccagcgcg tagcggatgg cgaggtaggt ggacccccag
    13801 atgatgtaca ccgccgccag cgcgagggcg acgcgggtgc ctgccacggt atgcgccttg
    13861 tccatctgtg cctgggtccc ggtgccgctg cctgaaacag gacgggcgcc gcagtggcgc
    13921 ccgtcggtgc ggccatcatc ggccggtcgt gtccgtgctc aatccaccca gcgggtgccg
    13981 ccgcgcgcct gcggcaacac ctgcgcggac agcccggcgt cggcgccgag caggccgatc
    14041 gcgtcgccga ggatcgccgc ggattcgcgc agcagcggat cgcgcgactt cttcgccgct
    14101 tcctcgcgcg ccacgtccgc ggcgacgttg cgttcgttcg cctgcaggcc gtcgtcggcg
    14161 gcttgctgtt ccagcagcgg atcctgggcg atgccgaggt tggcgcggac cgctgcgcgc
    14221 tgcttgcgca ggcggtcgaa gcggtcgcgc tcggcgcggc gctcggtctc gttcaacgac
    14281 accgacttct tcgcctgctc ctcgcggaac cgcgcgacgt cctccaccca ccactggaac
    14341 tcgacgtcgt tcgccacccg cgcggcatgg cgcgcctcca gcttgcccag cagcgaaccg
    14401 aagttgccgt actgcacgtg cggtgcggcg gcgatgcggg tccacggcag cgcgttgtcg
    14461 taggtgctct cgccgtattc gctggcatcc acgctggtcg ggaaggcaac gtccggcacc
    14521 acgcccctgt tctgggtgct gctgccgccg ggccggaaga actgggcgat ggtcagcttg
    14581 acctggccga agcgcagttc ctcgttggcc ggccagcggt cgaggtcgac catgctctgc
    14641 acggtgccct tgccgaaggt ggtctcgccg atcaccaggc cacggccgta atcctggatc
    14701 gcgcccgcca cgatttcgga agccgatgcg gagccacggt tgaccagcac cgccagcggg
    14761 ccgtcccagg cgacgcccgg gttgcggtcg ttctccacgc tgacccggcc gccggattcg
    14821 cgcacctgca ccaccgggcc ctggtcgatg aacaggccgg tcagctcgat cgcctcgctc
    14881 agcgagccgc cgccgttgcc gcgcaggtcc agcaccacgc cgtccacctt ctgcgcgcgg
    14941 aactgctcca gcatgcgggc gacgtcgcgg gtcgcggagg cgtagtcgcc gtcacggcgg
    15001 cggcgcgcct ccacgtcctg gtagaacccg ggcagcttga tcacgccgat ccggcgcgcg
    15061 ggctggccac cggcggccgg cagcagcacc tcctcggcct tggcgcgcgc gtcctccagc
    15121 cgcaccttgg cgcgggtgat caggatccgg gtcggcttgc tgtccagcgg cgcctcggcg
    15181 gagaccacgt ccagccgcac ctgggtgttg gcggcgccgc ggatcaggtc gaccacgtcg
    15241 tcggtgggcc agccgaccac gtccttcatc tcgccggagc cgccctggcc gacgccgacg
    15301 atgcggtcgc cggagcgcaa cttgccgccg cgcgcggccg gcccgcccgg caccatctcg
    15361 cgcaccacca cgatgtcgtc ctgcttgaac agcaccgcgc cgatcccctc cagcgagttg
    15421 gagatctgca cgttgaagtt ggccgcgctg cgcggggtca tgtagtcggt atgcggatcg
    15481 atcgccccgg cataggcgtt gaggaagctc tggaacacgt cctcgccctt gagctcggcg
    15541 atgctgtcca gcaggttcgc gtagcgcttg tcgagggtct tgcggatttc ctccggcggc
    15601 ttgccggcca gcttcaggcg cagccagtcg ttcttgaccg actgcttcca cagcgcatcc
    15661 agctcggcgc cgctggccgc ccacggggcg tcctcgcggt cgtattcgta acgctcgccg
    15721 ccggcgaagt cgaagttgcc cttcagcagc ccgcgcgcat gggcgatgcg ctcaccgacg
    15781 cgctgccggt acaccgcgaa catcgcccac gccggatcga cctcgccgct cttgatcgca
    15841 tcgtcgaggc gggtggcgaa cttcgagaaa ccggcgacgt cctgcgcggt caggaacacc
    15901 ttgcccgggt cgagggtgtc gaggtagcgc tcgagcacgt cctgcgacaa cgcgtcgtcg
    15961 agcacgcgcg gccgataggc gtagcgactg tcggacagca ggccatgcac cagccgcgcg
    16021 gtggtcgcct ggttgctggt cggggccgcg ggcagggcat cgcggctgcc ctccgcgcgc
    16081 gccagcaggg cgagcggcgc ggccagggcc aggccaacgg caagtgcaag cagggtgtgg
    16141 ctggacttca tggtgacttc cgcggcgtgg gggccggcat ggctgcagga tgggcccgga
    16201 tgggacttcg gcacagtgcc ggaagttcgg aaacagcggg cgtgccgcca ctgtagcggg
    16261 cttgcggcgg gcagtgtgaa cggcgcgatg aatgccatcg ggcccgcctg cgggcctggc
    16321 ggtggctgcc gctggcctgt gggtaccgtc cggcgcgacc ccgccggttc aggccagccc
    16381 ggcagcctgg cgatccgcgt gatagctgga acgcaccatg ggcccggatg caacgtgcga
    16441 gaacccgatc gaatacccgt agtcctcgag cgccttgaac tcgtccgggg tccagtagcg
    16501 cagcagcggg tggtgggcgg gcgacggctg caggtactgg ccgatggtga ccatctccac
    16561 gtcatgcgcg cgcaggtcgc gcagggtcgc ctgcacctgc tccatggtct cgcccaggcc
//
LOCUS       DAORUK010000031        16444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_3240_length_16444_cov_18.751053, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000031 DAORUK010000000
VERSION     DAORUK010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 16444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16444
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3240_length_16444_cov_18.751053"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PK818_03470"
     CDS             complement(<1..241)
                     /locus_tag="PK818_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HQY81826.1"
                     /translation="MIVRARAPLRLGLAGGGTDVAPYCDLHGGFVMNAAIDKHAHATV
                     DASPDGMLVLEALDRGVSASCLPGDLRADAGPLQLL"
     gene            complement(238..3936)
                     /locus_tag="PK818_03475"
     CDS             complement(238..3936)
                     /locus_tag="PK818_03475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY81827.1"
                     /translation="MSLARAPGAKGLLVLGMHRSGTSALTRLLNLHGAVLGDDLLPAG
                     HDNPSGFWELREAVAIHERLLAGLGMAWDDPRALPPDWQDGDAARRAGDAIGALIDRT
                     FAGEALWAVKDPRLCRFAPLWSRAMRARGIEPHAILVARHPAEVAHSLQTRDALPAAL
                     GNLLWARHFVDAVNGSGDMPRTLLRYPDLLQDWRGAVAGIEHALAVELRHDPAIEAAA
                     DAFLVPQMRHHVVDAGQSADGLVAPLQAALDQASDNTLPSAWPTAVDRFSQVLAPVEG
                     VVDGLAGMLSTSRAQLAAARDETAGLHGELDARGSWAASLDAELNGLRATHGALVAEH
                     AGAVAWAQSLQGELAALGEIHRKVDADRVEKLAWAISLRHQLEALGDNCQVLQADLDE
                     KISWANALQVQLDELGRTHRALQADRDEKLEWAQATRDELEQLRQAYQRVDSDRLEKL
                     AWARSLDAELVTSREATRQLNERLGVSEAEAMQLREQAADLVLDLRKTQDALADSRAR
                     ATALDTYATALENAARALLASTSWKLTRPLRALRARLRGTGETLQLPQRPALPAYTPV
                     PTASVSPQVDMPALLEGVHFPDVPAPRVTIVVPTYGKLDYTARCLRALQASGDAAMFE
                     VLVLEDASGDAGMTALRDVPGLRYHENPQNLGFLRSCNQALQLARGEYVCFLNNDTEP
                     MPGWLDALLDVFAQHADAGMAGSMLLYPDGRLQEAGGILWRDASAWNYGRLGDPTATE
                     FNYVRRADYCSGAALLLPSALFRELGGFDERYVPAYCEDSDLAFRVREAGREVYFTPF
                     SKVVHFEGISHGTDTGSGIKACQVANQARFRERWAKELAAHYPNGEQVARARDRAWDR
                     PVVLVVDHYIPQPDRDAGSRTMFAFLQRLLEAGCVVKFWPDNLHYDPQYAPRLQAMGI
                     EVLHGPRWLGGIGALVEACGDIFDAVLLSRPDVAEHHLAATRAHSRARVVYYGHDLHF
                     RRMQDQAALLDGGADRDALQREAASMEARERAIWRGVDAVLYPSADEADAVRALEPAV
                     DARAVTPYAYAAFGSETPLAQRGDILFVAGFAHPPNVDAARWLVEAIMPRVWATHPQA
                     RLALVGANPTADVLALACDRVEVTGFVSDDALARRYRHARVAVVPLRYGAGVKSKVVE
                     ALQQGLPLVTTTVGAQGLPGVEAACSVADDIDGIAGGIVRLLDDDAAWLRCSRDGSAY
                     ASAHFSADAMRRDLLSALAIDARTEHGA"
     gene            complement(4000..6150)
                     /locus_tag="PK818_03480"
     CDS             complement(4000..6150)
                     /locus_tag="PK818_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HQY81828.1"
                     /translation="MNNLRPLLIAAATALLAGACATPPVVLPAPLPLPDQAPGTEAFS
                     QQQGGVQRQDLQRSGTPEIPGSGNNTLVAGISGAAMPPLKGGAVNVNIEGMPVPAFIN
                     EFFGSILGVGFQMDPQVARMQDLVTLRTQGPQSPQDFYRLAVQVLRTYGVSTEFSSGL
                     VFFNRARDSSTGATPLVVSGRTLPEVPISHRPVFQLMEMHSVRASDVVNLLNVAFKDQ
                     GLTIQADSGRNAVILMGKPELVRQAGEAIRVLDRPYMRGRVSARLEPAFVTAADLAKR
                     LVEVMSAEGYGAALYSAGAGGTAILVLPLDAANAVLVFAADRGALEHAVEWARSIDKP
                     NPTAGSDGLFYYMVKNTQAAEIVKTITGVRTGTGGTRDPARNAIDGSQVGAAPPAAGT
                     ATNAAAGGGDLAKGKLSLDEPRNAIIFQGSATDWGRVLQLIQQMDRAPRQVMIEVTVA
                     EVTLTNDQELGIAWLARNADLGKYNGTISSGTLVTGTGGAGLTYLLDIGGQTRASLRA
                     LASQDRISVLSTPRLMVKSGEEASFDVGTEVPTISSQATSGITTDGNSGIIQTVQYRK
                     TGILLNVKPVVYSDDRVDIELRQEVSEALPVGADSSVNSPAIFNRSYTTSLSLKDGSA
                     ILIAGLMAQRKTVGDSGVPYLKDIPLLGNLFKTEKRGDNKTELIMMIVPYILETDDQV
                     EELTRSLGQRFEMLELPLTGQPPVQVTPSVPVQP"
     gene            complement(6175..6750)
                     /locus_tag="PK818_03485"
     CDS             complement(6175..6750)
                     /locus_tag="PK818_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81829.1"
                     /translation="MALPELVRRRGLAAAALAGVLVGAVIGLFLPIRAAAPPKPDDTP
                     WSLPNAQETRRFRFDQFQSVQSARYWGDLRQAGSRAAPATQWTLAAIVTQPRLRVAVN
                     ESGKAQKSVWVGIGETMPDGATLVAANRDSIWFEKDGCRRLRKLYQKPTAESDACIGA
                     PGKPAATPSSGKPAVVPAKPSPAAPVARGPN"
     gene            complement(6737..7345)
                     /locus_tag="PK818_03490"
     CDS             complement(6737..7345)
                     /locus_tag="PK818_03490"
                     /inference="COORDINATES: protein motif:HMM:NF022201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HQY81830.1"
                     /translation="MSARIPSQLRAMREEWKGNPRLRWGTVAAAAIAFIYLCLVLVDW
                     RRDLHSHYQERSLHLYKMTALAGQEYWVARAQSAQAVEKALQAEIPAAATIGLAQAEV
                     QSMVRKLMNAYGRKLTTEARPPTELAGQRGLWRIPVTMRGVTSQAQMLEILRLLESSD
                     RLVTIEQFSFTFAQGVPNFSLTLVAYYRVGKLGVQGAANGAA"
     gene            complement(7342..8313)
                     /locus_tag="PK818_03495"
     CDS             complement(7342..8313)
                     /locus_tag="PK818_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81831.1"
                     /translation="MLARGFCTYTVLDGAAVPVRKRRGFVEMAVARWSPFADVQSHVE
                     WVGDRAMVWAWSKSQVEAVDGVESRPSPRRARPESLFRGEPRASGDELVTLEEGFEGR
                     VWRDGVMTASCWWPQVPSLGEWNEFRRGAGLPPEAAAPVAVASPLADRAWTTPKAIGV
                     GEAFGRYGGMLALAAVGIGTAVVCALLVGVLALKVSIWQLDRDIAEREQSLERIIDAR
                     DGAMKARAAIDARIAMRPPAGQVELLALVSGLISGNWQLLEWKVPDAQTLEMTARMAN
                     PDPRAIVSAWEGSGRFSAVTAEIGRQPDSVVVKARILRAPLRKGTGK"
     gene            complement(8357..9433)
                     /locus_tag="PK818_03500"
     CDS             complement(8357..9433)
                     /locus_tag="PK818_03500"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HQY81832.1"
                     /translation="MRPVRREGGFVLVLVLAMLVVLSLLAGSIAATTSRLLEQATQRE
                     RGMQDQLDMASTRATVLYLIGTQRLTVGGLTVDNLVSFGEDGVRPSQSPADIEGNGLL
                     LPVGTEIAMDGRAYRGIGNARFAIQDENGLFAVNWNSPARLERLLAQGGQQRPVAAPA
                     LFNRIMDYQDRDNLYRLDSLEADGYRSAGMLPPTNLPLATPMELFRVAGWEQALSFLT
                     PVDVSDTLTVESVSVLNVNTAPARVLGTLPGVDQEMAERAIAYRKLQPFMTDTAFFRF
                     LGLQPTLDAPVAVYPARSGTLKLWPSQGGQVRLLHWRSTPSEDRGRPWREDYELIQSR
                     ASSRNDAAYPVRSRLFREPVAARK"
     gene            complement(9430..10035)
                     /locus_tag="PK818_03505"
     CDS             complement(9430..10035)
                     /locus_tag="PK818_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HQY81833.1"
                     /translation="MPSRPGRTAAGFTLMEMLVTLMLVSFATMLMFQMLGSYRIANDR
                     VREQAGSIDRRALFQAWFRDSVQGLYAAPNLTFVGAPDRFAATTLNPLYAPAGRPTPI
                     EWRLRTSTDGLPEVVYVEAGLERWSLPLKGARAATFAYYAEDWRQLDTWPPNRGKVQP
                     GALPALVALVREAADGERPVLAAVLGPLEPPRRLYGEEEYE"
     gene            complement(10053..10559)
                     /locus_tag="PK818_03510"
     CDS             complement(10053..10559)
                     /locus_tag="PK818_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HQY81834.1"
                     /translation="MRGQVTRNRESGFNPALGHSPRAFSRPGGSAANAAGFTLLEAIV
                     ALVVFSMGAMALYGWLSTNIITLNRIQERQQVEVAQLSALDMIRRTNPMEIPSAQRQA
                     GSLQVAWTSVPVEPPRPNAHRDGSPGIFLVGLYDAHVRVLRDGRQLQAFRVRQVGWKQ
                     VQTLGDDQ"
     gene            complement(10559..10945)
                     /locus_tag="PK818_03515"
     CDS             complement(10559..10945)
                     /locus_tag="PK818_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HQY81835.1"
                     /translation="MRRDGFTLLEMIVVLAILGLATAMVAPAAIRSIDSWRRQAELDS
                     LLDQIRALPGDARASGRVIVLDGEALKGKDAPLRVAGDWTLRVPEPWKVNANGVCEGG
                     SVEIGNQHGKRVIAVASPFCDPRVKP"
     gene            complement(10956..11420)
                     /gene="gspG"
                     /locus_tag="PK818_03520"
     CDS             complement(10956..11420)
                     /gene="gspG"
                     /locus_tag="PK818_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HQY81836.1"
                     /translation="MHRRRIPSIQRQAGFTLLEMIVVLVIIGLIMGLVGPRLFGQADK
                     AKVQTAQTQIKMLRGALQTMRLDISRWPTEQEGLALLTTRPASAEAVPGWAGPYLDEA
                     VPQDPWGQPYQYAPQATGDNPFVLYSWGADAKAGGEGQDADLGYLPDQAGTP"
     gene            complement(11451..12647)
                     /locus_tag="PK818_03525"
     CDS             complement(11451..12647)
                     /locus_tag="PK818_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQY81837.1"
                     /translation="MPRYHYQAMNAEGISLEGMLRANNEREAARALERRGLSVIEVRS
                     GEAGGGNERRGRLRHGDVILALQELATMLTSGVSIADAVGSQALGAHHPRIVAAFAGM
                     SRDLQRGQAFSATLAKCGLPLPEYVVQLARAGEMTGELGKALRDAGAQMEYEQQLRNE
                     MRNALIYPAVLVVAGIAAVAIMFIFVVPKFASLLEQANDLPLLAWVVLAFGTWTRENF
                     WLLLLLLAAAGAGAAWALRKPELRARTMDALSRWPVVGPWLVEADTARWAKVLGALLG
                     NKVPLMRGLELAQSGLQLPQRHARMGEVTRAVRGGASLADALEDHDALTATGYNLIRV
                     GERSGKLASMLDSLARLYEEAGRNRMKRVLILLEPIAILVIGSMIGTIILGVILAITS
                     ANDLAV"
     gene            complement(12652..14355)
                     /locus_tag="PK818_03530"
     CDS             complement(12652..14355)
                     /locus_tag="PK818_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HQY81838.1"
                     /translation="MSSDAALRVAPRDRLLGELLLDAGLVGPADLERGLALQLKLGGR
                     IGSVLMRIGAVSEDNLLQVLSRQLGLPVMGADVPAPDEDMVRLTAAQAPTGIDWLLDQ
                     QLLVWPGEGSDLFCAARDPLLPALREAMRHAYPGRKVLWCLCRAQDLERMLDALAHHA
                     RSDEWGGGDEQQLRAMAEEAPVVELVSNLMAQAVEQRASDIHLEPEERIFAVRFRIDG
                     VLHTRLQLPRDRFDAVASRLKLISGMDIAERRLPQDGRLSTRVGGQEMDIRVSALPGV
                     HGESIVMRLLPKERKELGLERLGFESDHLAMMQAWTAEANGIVLVTGPTGSGKSTTLY
                     AALAAANDGLKKIITVEDPVEFQLPAITQIQAHPDIGLTFANALRSILRQDPDVIMIG
                     EIRDLETAEIAVQAALTGHLVLSTLHTNDAISAFTRLIDMGVEPFLVATPIRAVQAQR
                     LVRRLCTQCARPHAVPHNIEAEAAVFAHKVLPGMAPRWMEAVGCPNCMNTGYRGRLGI
                     YEMVPVSEAMQHLIVSGASVNEMKKLARDEGHRFLRDDGLLKAWQGLTTVEEVLRVAG
                     T"
     gene            complement(14345..15145)
                     /locus_tag="PK818_03535"
     CDS             complement(14345..15145)
                     /locus_tag="PK818_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY81839.1"
                     /translation="MQASLSHPWRHRALIRILAWREISGRYRGSLLGSLWSLLTPLLM
                     LGVFTLVFGVIAPTRWPGAEAQGIGMFALRLLAGMVVHGLLAEVLSRAPTLVTGQPNY
                     VTKVVFPLETLGWVSLLAGLFHAAMALLVLVALNGLLGTGLSWSLLALPLVLLPFAVL
                     LLGLAWLVAALGVYLRDLAQLIGPLVMVTMFLGPVFYPREAMPAAAQPWLAVNPITIP
                     VEQLRRVLFAAQWPQWDVVAQYSLVAVAVYLFGLWAFTKLKKGFADVL"
     gene            15618..>16444
                     /locus_tag="PK818_03540"
     CDS             15618..>16444
                     /locus_tag="PK818_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81840.1"
                     /translation="MKKNVLAVALVAGLGLAGGAAAYNYGTSLADDSAAAAFSDAALQ
                     DAENVSYEMLVASAYKWTMKEGLVFDIETADNGAAVTEGFTVRVALNQRCSPHDTATN
                     PASPPTNVGVCAPTGDGAQFDASIPIVAGDLVLDPTLALEWKVTFDGYTSGNTVATFR
                     IEPLVTNPTGPGTNGTTITFTWNNAHLTSLNEFAATAAAGGNAKVDAEFWMVNVSNDA
                     RFVNSTRSRTILESRNGVVACATPNPAEIDKYIDVADNFNEDQIPKTRFSWDGKLGSA
                     "
BASE COUNT         2584 a         5979 c         5418 g         2463 t
ORIGIN      
        1 cgagcagttg cagcggcccg gcatcggcgc gcaggtcgcc cggcaggcag gacgccgaca
       61 cgccgcgatc cagcgcctcc agcaccagca tgccgtccgg cgacgcatcg accgtcgcgt
      121 gcgcgtgctt gtcgatcgcc gcgttcatca cgaagccgcc atgcaggtcg caatagggcg
      181 ccacatcggt gccgccaccg gccagcccca ggcgcagcgg cgcgcgcgcg cggacgatca
      241 tgcgccgtgc tccgtgcggg cgtcgatggc cagcgcagac agcaggtcgc ggcgcatggc
      301 atcggcggag aaatgggcgg aggcatacgc gctgccatcg cgcgagcaac gcagccaggc
      361 cgcgtcgtcg tcaagcaggc gcacgatgcc gccggcgatg ccgtcgatat cgtcggccac
      421 cgaacaggcc gcctcgacgc cgggcaaccc ctgcgcgccg accgtggtgg tgaccagggg
      481 cagcccttgc tgcaacgctt cgacgacctt gctcttcacc ccggcaccat agcgcagcgg
      541 caccactgcg acccgcgcat gccggtagcg ccgcgccagt gcatcgtcgc tgacgaaacc
      601 ggtgacctcg acccggtcac aggccagggc aagcacgtcg gcggtcggat tcgcgcccac
      661 cagcgcgagg cgcgcctgcg gatgggtggc ccacacccgc ggcatgatcg cttcgaccag
      721 ccagcgcgcg gcgtccacgt tcggtggatg cgcgaagccg gcgacgaaca ggatgtcgcc
      781 gcgttgcgcg agcggggttt cggagccgaa cgcggcgtag gcgtaaggcg tcaccgcgcg
      841 cgcatcgacg gcaggctcca gcgcacggac cgcatcggct tcgtcggccg atggatacag
      901 caccgcatcc acgccgcgcc agatcgcgcg ctcgcgcgct tccatcgatg ccgcctcgcg
      961 ctgcaacgca tcgcggtccg cgccgccatc cagcaacgcg gcctggtcct gcatgcggcg
     1021 gaaatgcagg tcgtgcccgt aatagacgac acgcgcgcgg ctgtgggcgc gggtcgcggc
     1081 caggtggtgc tcggccacgt cgggccgcga caacaacacc gcgtcgaaga tgtcgccgca
     1141 ggcctcgacc agcgcgccga tcccgcccag ccagcgcggg ccgtgcagca cctcgatgcc
     1201 catcgcctgc agccgcggcg cgtactgcgg gtcgtaatgc aggttgtccg gccagaattt
     1261 caccacgcag cccgcctcca gcaaccgctg caggaaggcg aacatggtgc gcgaaccggc
     1321 atcgcgatcc ggctgcggga tgtagtggtc gaccaccagc accaccggcc ggtcccaggc
     1381 gcggtcgcgc gcgcgcgcga cctgctcgcc gttcggatag tgcgcggcca gctccttcgc
     1441 ccagcgttcg cggaacctgg cctggttggc gacctggcag gccttgatcc cgctgccggt
     1501 gtcggtgccg tgcgagatgc cctcaaagtg caccaccttc gagaacgggg tgaagtacac
     1561 ctcccggccc gcttcgcgta cccggaacgc gaggtccgaa tcctcgcagt aggcggggac
     1621 gtagcgctcg tcgaaaccgc cgagttcgcg gaacagtgca gacggcagca acagcgccgc
     1681 gcccgagcag taatccgcgc ggcgcacgta gttgaattcg gtcgcggtgg ggtcgcccag
     1741 ccggccatag ttccacgccg aggcatcgcg ccacaggatg ccgccggctt cctgcaggcg
     1801 tccgtccgga tacagcagca tcgagccggc catgccggcg tcggcgtgct gcgcgaacac
     1861 gtcgagcaac gcgtccagcc agcccggcat cggctcggtg tcgttgttga ggaagcagac
     1921 gtactcgcca cgcgcgagct gcagggcctg gttgcaggaa cgcaggaagc cgaggttctg
     1981 cggattctcg tggtagcgca gcccggggac gtcgcgcagc gcggtcatcc cggcatcgcc
     2041 ggaggcgtcc tccagcacca gtacttcgaa catcgcggca tcgccactgg cctgcagcgc
     2101 gcgcaggcaa cgcgcggtgt aatcgagctt gccgtaggtc ggcaccacga tggtcacgcg
     2161 cggcgcgggc acgtccggga aatgcacgcc ttccagcagg gccggcatgt ccacctgcgg
     2221 cgaaacggac gctgtgggta ccggcgtgta cgccggcagc gcgggacgct gcggcagttg
     2281 cagggtttcg ccggtgccgc gcaggcgcgc gcgcaacgcg cgcagcggac gcgtgagttt
     2341 ccaactggtg gatgccagca atgcgcgcgc tgcgttctct agcgcggtgg cgtacgtgtc
     2401 cagtgcagtg gcgcgcgcgc ggctatcggc caacgcgtcc tgcgtcttgc gcaggtccag
     2461 caccaagtcc gcggcctgct cgcgtagctg catcgcctcg gcctcgctca cacctagccg
     2521 ctcattgagc tggcgcgtcg cttcacggga tgtcacaagc tccgcatcca gcgaacgcgc
     2581 ccatgccagt ttctccagcc ggtccgaatc cacccgctgg tacgcctggc gcagctgctc
     2641 cagctcgtcg cgcgtggcct gtgcccactc gagcttctca tcacgatccg cttgcagtgc
     2701 gcggtgggtt cgccccagtt cgtccagctg cacttggagg gcgttcgccc aactgatctt
     2761 ctcgtcgaga tccgcctgca ggacctggca gttgtcgccc aacgcctcta gctggtgccg
     2821 cagcgatatc gcccaagcga gtttctcgac gcgatccgca tccaccttgc gatggatctc
     2881 gcccagcgcg gccagttcgc cctgcagcga ctgcgcccag gccaccgcac ccgcgtgttc
     2941 ggcgaccagc gccccatggg tggcgcgcaa cccattgagt tccgcatcca gcgacgcggc
     3001 ccagctgccg cgtgcgtcca gttcgccgtg caggccggcg gtctcgtcgc gtgccgctgc
     3061 aagttgggcg cggctcgtgg acagcatgcc ggcaaggccg tccaccacgc cttcgaccgg
     3121 cgccaggacc tgcgagaaac gatcgacggc cgtcggccag gccgatggca gcgtgttgtc
     3181 gctggcctgg tcgagcgccg cctgcagggg cgcgaccagc ccatccgcgg attgcccggc
     3241 atcgaccacg tggtggcgca tctgcggcac caggaaggca tcggcggcgg cttcgatcgc
     3301 cgggtcgtgg cggagctcga cggcaagcgc gtgttcgatg ccggcgaccg cgccgcgcca
     3361 gtcctgcagc aggtccggat aacgcagcag ggtgcgcggc atgtcgccgc tgccgttgac
     3421 cgcatccacg aaatggcgcg cccacagcag gttgccaagc gcggccggca gcgcatcgcg
     3481 cgtttgcagc gaatgcgcca cttccgccgg atgccgggcg accaggatcg cgtgcggctc
     3541 gatcccgcgc gcgcgcatcg cccgcgacca cagcggcgcg aagcggcaca ggcgtgggtc
     3601 cttgaccgcc cacagcgcct cgccggcgaa ggtgcggtcg atcagcgcgc cgatcgcgtc
     3661 gcccgcgcgc ctggccgcgt cgccgtcctg ccagtccggc ggcagcgccc gcgggtcgtc
     3721 ccaggccatg ccaaggccgg ccagcagccg ttcgtggatc gcgaccgctt cgcgcagttc
     3781 ccagaagcca ctggggttgt cgtgccccgc cggcagcagg tcatcgccca gcaccgcgcc
     3841 gtgcaggttg agcagccggg tcagcgccga ggtaccgctg cggtgcatcc ccagcaccag
     3901 caggcccttc gcgcccggcg cccgcgcaag ggacacccgc ggttgtccat gctgtgctgg
     3961 catgcggtcc atccgctggt cgggaagctg ccgccgcgat cagggctgta ccgggaccga
     4021 gggcgtgacc tgcaccggcg gttgcccggt cagcggcagt tccagcattt cgaagcgctg
     4081 gccaagcgaa cgggtcaatt cctcgacctg gtcatcggtc tcgaggatgt acggcacgat
     4141 catcatgatg agttcggtct tgttgtcgcc gcgcttctcg gtcttgaaca ggttgcccag
     4201 caacgggatg tccttgaggt agggcacgcc gctgtcaccg acggtcttgc gctgcgccat
     4261 caggccggcg atcaggatcg cgctgccgtc cttcaggctg aggctggtgg tataggaacg
     4321 gttgaagatc gccggcgagt tgaccgagga gtccgccccc accggcagcg cttcgctgac
     4381 ctcctggcgc aactcgatgt cgacccggtc gtcggagtac accactggct tgacgttgag
     4441 caggatgccg gtcttgcggt actgcacggt ctggatgatg ccgctgttgc catccgtcgt
     4501 gatgccggag gtggcttgcg atgaaatcgt cggaacttcg gtgccgacgt cgaaactggc
     4561 ttcctcgcca ctctttacca tcagtcgcgg ggtggacagc acgctgatgc ggtcctggct
     4621 ggccagcgcc ctcaaggacg cgcgggtctg cccaccgatg tcgagcaggt aggtcaaccc
     4681 ggcaccgccg gtaccggtga ccagggtgcc ggagctgatg gtgccattgt acttgccgag
     4741 gtcggcgttc ctcgccagcc acgcgatccc cagttcctgg tcgttggtca aggtcacttc
     4801 ggccacggtg acctcgatca tcacctgacg cggtgcgcga tccatctgct ggatcaactg
     4861 cagcacgcga ccccagtcgg tcgccgagcc ctggaagatg atcgcgttgc gcggctcgtc
     4921 cagcgacagc ttgcccttgg ccaggtcgcc accgccggcc gcagcgttgg ttgcggttcc
     4981 ggccgcaggc ggcgccgcgc cgacctggct gccgtcgatc gcgttgcgcg ccgggtcgcg
     5041 ggtgccgcca gtacccgtgc gcaccccggt gatcgtcttc acgatctcgg cggcttgggt
     5101 gttcttgacc atgtaataga aaaggccgtc gctgcccgcg gtgggattgg gcttgtcgat
     5161 gctgcgcgcc cattccaccg catgctccag cgcgccgcgg tcggcggcga acaccagcac
     5221 cgcattcgcc gcatccagcg gcagcaccag gatcgcggtg ccgcccgcac cggcgctgta
     5281 aagcgccgca ccgtagcctt ccgcgctcat gacttcgacc agccgcttgg caaggtcggc
     5341 ggcagtgacg aacgcgggct ccagcctggc gctgacgcgg ccgcgcatgt aaggccggtc
     5401 gagcacgcgg atcgcttcac cagcctgccg caccagctcc ggcttgccca tcaggatcac
     5461 cgcattgcgg ccgctgtccg cctggatggt caggccctgg tccttgaatg ccacgttcag
     5521 caggttcacc acgtcgctgg cccgcaccga gtgcatttcc atcaactgga acaccgggcg
     5581 gtggctgatg ggcacctcgg gcagggtacg tccgctgacg accagcggcg tggccccggt
     5641 cgacgaatcg cgcgcgcggt tgaagaacac caggccggag ctgaactctg tggacacgcc
     5701 atacgtgcgc agcacctgca ccgcgagccg gtagaaatcc tgcggcgact gcggtccttg
     5761 cgtgcgcagg gtgaccaggt cctgcatgcg cgcgacctgc ggatccatct ggaagccgac
     5821 gcccaggatc gaaccgaaga attcgttgat gaacgccggt accggcatgc cctcgatgtt
     5881 gacgttcacc gcaccgccct tcagcggcgg catcgccgcg ccgctgatgc ctgcgaccag
     5941 cgtgttgttg ccgctgccgg ggatttccgg ggtgccgctg cgctgcaggt cctggcgctg
     6001 aacgcctccc tgctgctggc tgaacgcctc ggtgccaggc gcctggtccg gaagcggcag
     6061 cggtgcgggc agcacgaccg gcggcgtggc gcaggcgccg gccaacagcg cggtggcggc
     6121 ggcgatcaac aggggacgaa ggttattcat tcggatggcg tttcttcacg gaattcagtt
     6181 ggggccgcgc gccaccggcg ccgcgggcga aggcttggcc gggacgacag cgggcttgcc
     6241 cgatgatgga gtggccgcag gcttcccagg cgcgccgata caggcatcgg attcggcggt
     6301 tggcttctgg tacagcttgc gcaggcggcg gcaaccgtcc ttttcgaacc agatgctgtc
     6361 gcgattggcg gcgaccaggg tcgcaccgtc gggcatcgtc tcgccgatgc cgacccagac
     6421 cgacttctgt gccttgcccg actcgttgac cgccaccctg agccgcggct gggtgacgat
     6481 ggcggccagg gtccattgcg tcgcaggcgc ggcgcggctc ccagcctgcc gcaggtcacc
     6541 ccagtagcgg gcactctgga cggactggaa ctggtcgaag cggaaacgcc gggtttcctg
     6601 cgcgttgggc aacgaccacg gcgtatcgtc aggcttcggc ggcgcggccg cgcggatcgg
     6661 caggaacaag ccgatcaccg cgcccaccag cacgccagcg agcgccgcag cggccagtcc
     6721 acgccggcgc acgagctcag gcagcgccat tggccgcccc ctgcacgcca agcttgccca
     6781 cgcggtaata cgccaccagc gtcagcgaga aattcggcac gccctgcgcg aacgtaaacg
     6841 agaattgctc gatcgtgacc aggcggtcgg agctttccag caaccgcagg atctccagca
     6901 tctgcgcctg gctggtcacc ccccgcatcg tcaccggaat gcgccagagg ccacgttggc
     6961 cggccaactc cgtcggcgga cgggcttcgg tggtcagctt gcggccgtag gcgttcatca
     7021 gcttgcggac catcgactgc acttcggcct gggcaaggcc aatcgtcgcg gctgcgggaa
     7081 tctccgcctg cagcgccttc tccaccgcct gcgccgattg cgcgcgcgcc acccagtact
     7141 cctggccggc gagcgcggtc atcttgtaca ggtgcagcga ccgctcctgg taatgcgaat
     7201 gcaggtcgcg gcgccagtcc accagcacca ggcacaggta gatgaaggcg atggcagcag
     7261 cggcaacggt cccccagcgc agccgggggt tgcccttcca ctcttcgcgc atcgcccgca
     7321 gctgcgacgg gatccgggca ctcacttgcc ggtccccttc cgaagcgggg cacgcaggat
     7381 gcgcgccttg acgaccacgc tgtcgggctg ccggccgatc tccgccgtga ctgccgagaa
     7441 ccgcccggat ccttcccacg cggacacgat cgcgcgcggg tccgggttgg ccatgcgcgc
     7501 ggtcatttcc agcgtctgcg catcgggcac cttccactcc agcaactgcc aattgccgct
     7561 gatcaagccg gacaccagcg ccagcagctc gacctggccc gccggcggcc gcatcgcgat
     7621 gcgggcatcg atcgccgcgc gtgccttcat cgcaccgtcg cgcgcgtcga tgatccgctc
     7681 cagcgactgc tcgcgctccg cgatgtcccg gtccagctgc cagatcgaca ccttgagcgc
     7741 caggaccccg accagcagcg cgcacaccac cgcggtcccg atccccaccg ccgccagcgc
     7801 cagcatcccg ccgtaacgac cgaacgcctc gccgacgccg atcgccttcg gcgttgtcca
     7861 tgcgcggtcg gccaacgggc ttgctaccgc caccggtgcc gccgcctccg gtggcaaccc
     7921 ggcgccacgg cggaactcgt tccattcccc gagcgaaggc acctgcggcc accagcagga
     7981 tgcggtcatc accccatcgc gccagacccg gccctcgaag ccctcctcca gggtgaccag
     8041 ctcgtcaccg ctcgcccgcg gttcgccgcg gaacagcgac tccggccgcg cccgtcgcgg
     8101 cgagggccga gattcgacgc catcgaccgc ctccacctgc gacttcgacc acgcccacac
     8161 catggcgcga tcgcccaccc attccacgtg cgactgcacg tccgcgaacg gcgaccaccg
     8221 cgcgaccgcc atctccacga atccgcgccg cttgcgcacc ggcaccgccg cgccgtccag
     8281 caccgtgtag gtgcagaagc cgcgcgcgag cacccactcc tggccgtttc caccctcggc
     8341 cagctggcgc aggccgctac ttccgcgcag ccaccggctc gcggaaaagc cgcgagcgaa
     8401 ccgggtacgc agcgtcgttt cgcgacgagg ctcgggattg gatgagctca tagtcctcac
     8461 gccaggggcg accccggtct tcactggggg tcgatcgcca atgcaacagt ctcacctgcc
     8521 ccccctggga aggccacagc ttcaacgtgc ctgacctcgc aggatacacg gcgaccggcg
     8581 catccagcgt gggctgcagg cccaggaacc ggaaaaacgc cgtgtcagtc atgaacggtt
     8641 gcaacttgcg gtaggcgatc gcgcgctcgg ccatttcctg gtcgacgccg ggcagcgtcc
     8701 ccaggacgcg ggccggtgcg gtgttgacgt tcagtaccga caccgactcg acggtcaggg
     8761 tatcgctcac atcgaccggg gtcaggaacg acagcgcctg ctcccagccc gccacccgga
     8821 acagctccat cggcgtggcc agcggcaggt tggtcggcgg cagcatcccg gcgctgcggt
     8881 agccgtccgc ctccaggctg tccaggcgat agaggttgtc gcgatcctgg taatccatga
     8941 tccggttgaa cagcgccggc gccgccaccg ggcgctgctg gccgccctgc gccagcagcc
     9001 gctccagccg cgccggcgaa ttccagttca ccgcgaacag gccgttctcg tcctggatgg
     9061 cgaagcgggc attgccgatc ccgcggtagg cgcggccatc catcgcgatc tcggtgccca
     9121 ccggcaacag caggccgttg ccctcgatat ccgcgggcga ctgcgacggg cgcacgccgt
     9181 cctcgccgaa cgacaccagg ttgtccacgg tcaggccgcc aacggtcagc cgctgggtgc
     9241 cgatcaggta cagcacggtg gcgcgcgtgc tggccatgtc caactggtcc tgcatcccgc
     9301 gttcgcgctg ggtggcctgc tccagcaggc gcgacgtcgt cgcggcgatc gaccccgcca
     9361 gcagcgacag caccaccagc atggccagca ccagcaccag cacgaaaccg ccctcgcgcc
     9421 gcaccggcct cattcgtatt cctcctcgcc atacaggcgg cgcggcggct ccagcggacc
     9481 cagcaccgcc gccagcaccg ggcgctcgcc gtcggcggcc tcgcgcacca gcgccaccag
     9541 cgccggcaac gccccgggtt gcaccttgcc gcgattcggc ggccaggtat cgagttgccg
     9601 ccagtcctcg gcgtaatagg cgaacgtcgc cgcgcgtgcg cccttcagcg gcaggctcca
     9661 gcgctcgagc ccggcttcca cgtacaccac ctccggcaag ccgtcggtgc tggtgcgcag
     9721 ccgccattcg atcggggtcg ggcgccccgc cggcgcatac agcggattga gggtggttgc
     9781 ggcgaagcgg tccggcgctc cgacgaaggt caggttcggg gccgcataca gtccctgcac
     9841 gctgtcgcgg aaccaggcct ggaacagcgc ccggcggtcg atcgaaccgg cctgctcgcg
     9901 cacgcgatcg ttcgcgatgc ggtagctgcc cagcatctgg aacatcagca tggtcgcgaa
     9961 cgagaccagc atcagcgtga ccagcatttc catcagggtg aagccggcgg cggtgcggcc
    10021 gggccggctg ggcacccacg atccggggcc gctcactggt cgtcgcccag tgtctgcacc
    10081 tgcttccatc cgacctggcg cacccggaac gcctgcagct gcctgccatc gcgcagtacc
    10141 cgcacgtggg cgtcatacag cccgaccagg aagatgccgg gggacccgtc gcggtgcgcg
    10201 ttcggccgcg gcggctccac cgggacgctg gtccacgcca cctgcaggct gcccgcctgg
    10261 cgctgggcgc tggggatttc catcgggttg gtgcgccgga tcatgtcgag cgccgacaac
    10321 tgcgcgactt ccacctgctg gcgttcctgg atccggttga gggtgatgat gttggtcgac
    10381 agccacccgt acagcgccat cgcgcccatg ctgaacacga ccagcgcaac gatcgcttcc
    10441 agcagggtga agccggccgc gttggcggca ctgccgcctg gccggctgaa cgcccgaggg
    10501 ctatgcccga gggccggatt gaagccgctt tcgcgattcc tcgtaacctg gccgcgcatc
    10561 acggcttcac ccgcgggtcg cagaacggcg acgccactgc gatcacgcgc ttgccgtgct
    10621 ggttgccgat ttccacgctg ccgccttcgc agacgccgtt ggcgttgacc ttccacggct
    10681 ccggcacccg cagcgtccag tcgccggcga cccgcaacgg cgcgtccttg cccttcagtg
    10741 cttcgccatc gaggacgatc acccggccgc tggcgcgcgc gtcgccgggc agcgcgcgga
    10801 tctggtccag cagcgaatcc agttccgcct gccggcgcca gctgtcgatg ctgcggatgg
    10861 ccgccggcgc gaccatcgcg gtggccaggc caaggatggc cagcaccacg atcatctcca
    10921 gcaaggtgaa gccatcccgg cgcatgcgcg cggcgttacg gggtgccggc ctggtccggc
    10981 aggtagccga ggtcggcatc ctggccctcg ccgccggcct tggcgtcggc gccccaggaa
    11041 tacagcacga acgggttgtc gccggtggcc tgcggggcgt actgataggg ctggccccac
    11101 ggatcctgcg gcaccgcctc gtcgaggtag ggaccggccc agccgggcac cgcttccgcg
    11161 ctggcgggac gtgtggtcaa cagcgccagg ccttcctgct cggtcggcca gcggctgatg
    11221 tcaaggcgca tggtctgcag ggcgccgcgc agcatcttga tctgggtctg cgcggtctgc
    11281 accttggcct tgtcggcctg tccgaacagg cgcggaccga ccaggcccat gatcaggccg
    11341 atgatcacca gcacgacgat catctccagc agcgtgaagc cggcctggcg ctggatggaa
    11401 ggaatgcggc gacgatgcat cggtaatgcc cctgagtggt tgcgttgcgg tcagaccgcg
    11461 aggtcgttgg cgctggtgat ggccaggatc acgccgagga tgatggtgcc gatcatgctg
    11521 ccgatcacca ggatcgcgat cggctccagc aggatcagca cgcgcttcat ccggttgcgg
    11581 cccgcctctt catacagcct ggccaatgaa tcgagcatgg acgccagctt gccggagcgc
    11641 tcgccgaccc ggatcaggtt gtagccggtg gcggtcagcg cgtcatggtc ctccagcgca
    11701 tcggccagcg aggcgccgcc gcgcaccgcg cgggtgacct cgcccatccg cgcgtgccgc
    11761 tgcggcaact gcaggccgga ctgggccagc tccagcccgc gcatcagcgg caccttgttg
    11821 cccagcaggg cacccagcac cttcgcccag cgcgcggtgt cggcctcgac cagccacggc
    11881 ccgaccaccg gccagcgcga cagcgcgtcc atcgtccgcg cacgcagttc cggcttgcgc
    11941 agcgcccacg cggcgccggc gccggcagcg gcgagcagca gcagcaacag ccagaagttc
    12001 tcgcgggtcc aggtgccgaa cgccagcacc acccacgcca gcaacggcag gtcgttggcc
    12061 tgttccagca gcgaggcgaa cttcggcacc acgaagatga acatgatcgc gaccgcggcg
    12121 atcccggcca ccaccagcac cgccgggtag atcagcgcat tgcgcatctc gttgcgcagc
    12181 tgctgctcgt attccatctg cgcaccggca tcgcgcagcg ccttgccgag ctcgccggtc
    12241 atctcgcccg cgcgcgccag ctgcaccacg tattcgggca acggcaggcc gcacttggcc
    12301 agggtcgccg agaacgcctg cccgcgctgc aggtcgcgcg acatcccggc gaacgccgcc
    12361 acgatccgcg ggtggtgcgc acccagcgcc tggctgccga ccgcatcggc gatgctgacc
    12421 ccggaggtca gcatggtcgc cagttcctgc agggccagga tcacgtcgcc gtggcgcagc
    12481 cgcccgcggc gctcgttgcc gccgccggcc tcgccggagc gcacctcgat caccgacagc
    12541 ccgcgccgct ccagcgcgcg cgcggcctcg cgctcgttgt tggcgcgcag catgccttcc
    12601 aggctgatgc cctcggcgtt catcgcctgg tagtggtagc gcggcatcgg ctcaggtccc
    12661 cgccacgcgc agcacttcct ccaccgtggt caggccctgc cacgccttca gcaggccgtc
    12721 gtcgcgcagg aagcggtggc cttcgtcgcg cgccagcttc ttcatctcgt tgacgctggc
    12781 gccgctgacg atcaggtgct gcatcgcctc cgacaccggc accatctcgt agatgcccag
    12841 gcgcccgcgg tagccggtgt tcatgcaatt ggggcagccc accgcctcca tccagcgcgg
    12901 cgccatgccc ggcagcacct tgtgcgcgaa caccgcggcc tcggcttcga tgttgtgcgg
    12961 caccgcatgc gggcgcgcgc actgcgtgca caggcgccgc accagccgct gcgcctgcac
    13021 cgcgcggatc ggggtggcga ccaggaacgg ctccacgccc atgtcgatca ggcgggtgaa
    13081 cgcgctgatc gcatcgttgg tgtgcagcgt gctcaataca aggtgtccgg tgagcgccgc
    13141 ctgcaccgcg atctcggcgg tctcgaggtc gcggatctcg ccgatcatga tcacgtccgg
    13201 gtcctggcgc aggatcgaac gcaacgcatt ggcgaaggtc aggccgatgt ccgggtgcgc
    13261 ctggatctgg gtgatcgcgg gcagctggaa ttcgaccgga tcctcgaccg tgatgatctt
    13321 cttgaggccg tcgttggccg cggccagcgc cgcgtacagc gtggtcgact tgcccgaacc
    13381 ggtgggaccg gtgaccagca cgatgccgtt cgcctccgcg gtccacgcct gcatcatcgc
    13441 caggtggtcg gattcgaagc cgagccgctc cagccccagt tccttgcgct ccttcggcag
    13501 caggcgcatc acgatcgatt cgccatgcac gccgggcagc gccgacacgc ggatgtccat
    13561 ctcctggccg ccgacgcggg tggacaggcg cccatcctgc ggcaggcggc gttcggcgat
    13621 gtccatcccg gaaatcagct tgaggcgcga ggcgaccgcg tcgaagcggt cgcgcggcag
    13681 ctgcaggcgg gtatgcagga cgccgtcgat gcggaagcgc accgcgaaga tgcgctcctc
    13741 cggctccagg tggatgtccg aggcgcgctg ctcgaccgcc tgcgccatca ggttgctgac
    13801 cagctcgacg accggcgctt cctccgccat cgcgcgcagc tgctgttcgt cgccgccgcc
    13861 ccactcgtcg gaacgcgcgt ggtgggccag cgcgtccagc atccgctcca ggtcttgcgc
    13921 gcggcacagg caccagagca ccttgcgccc cgggtaggcg tggcgcatgg cttcgcgcag
    13981 cgccggcagc aacgggtcgc gggcggcgca gaacaggtcg gagccctcgc ccggccacac
    14041 cagcagttgc tggtcgagca gccagtcgat ccccgttggc gcctgcgccg cggtgaggcg
    14101 gaccatgtcc tcgtcgggcg ccggcacgtc ggcccccatc accggcaggc ccagctggcg
    14161 gctgagcacc tgcagcaggt tgtcctcgct gaccgcgccg atccgcatca gcacgctgcc
    14221 gatccgcccg ccaagcttca gctgcagggc cagcccgcgt tcgaggtcgg cggggccgac
    14281 cagcccggca tcgagcaaca gctcgcccag caggcggtcg cgcggggcga cccgcaatgc
    14341 ggcgtcagag gacatcggcg aatcccttct tgagcttggt gaacgcccac aggccgaaca
    14401 ggtacacggc cacggcgacc agcgaatact gcgccacaac gtcccattgc ggccactgcg
    14461 cggcgaacag cacccgccgc agctgctcca ccgggatcgt gatcgggttg accgccagcc
    14521 acggctgcgc cgccgccggc atcgcctcgc gcgggtagaa caccgggccc aggaacatgg
    14581 tgaccatcac cagcgggccg atcagctgcg ccaggtcgcg caggtacacg ccaagcgcgg
    14641 ccaccagcca ggccaggccc agcagcagca ccgcaaacgg cagcagcacc agcggcaacg
    14701 ccagcagcga ccatgacagc ccggtgccca gcaggccatt cagcgccacc agcaccagca
    14761 gcgccatcgc cgcatggaac agcccggcca gcaggctgac ccagcccagc gtctccagcg
    14821 ggaacaccac cttggtgacg tagttcggct gcccggtgac cagggtcggc gcgcgcgaca
    14881 gcacctccgc cagcagccca tgcaccacca tcccggccag caggcgcagc gcgaacatcc
    14941 cgatcccctg cgcttccgcg cccggccagc gggtcggcgc gatcaccccg aacaccaggg
    15001 tgaacacccc cagcatcagc agcggcgtca gcagcgacca cagcgacccc agcaacgagc
    15061 cgcggtaacg cccgctgatc tcgcgccagg ccaggatgcg gatcagcgcc cgatggcgcc
    15121 agggatggga caggctggct tgcacgcgga agggtcgatt ctctggggcc aacggccgga
    15181 tcggatggac aagcggttcc ccgggtggcc aggcggcacc gaagcgggcg gcgcaagtct
    15241 acctgcgcac ccgcctgccc ggccgattca ccctgaacag cgcctgacat gccccggacc
    15301 gtcgggtttc gcccgtaacg tcatgatgtg acgcaacttc cgctcaggac gccaacctgt
    15361 tcagaatact ggggattgac gattccttaa ccctgcgtta gcgtccgcat tgcaacttga
    15421 tcgaccaggg gagtaggggg agctgcgtct ggcctgtggc cagcgcggaa tccgtatcag
    15481 atacgtgaga atgcagtacc tggaagacac cggttgcggg gggccctgcc tcctggtacc
    15541 gggctagggc cagcaatcgg aacaagttgc gtcattccgc tttaccaact gacaggagtt
    15601 agttagacat gtctagcatg aagaagaacg tcctcgccgt tgccctcgtg gccggtctgg
    15661 gcctggccgg cggcgctgcg gcctacaact acggcaccag cctggccgat gattcggcag
    15721 ctgctgcttt ctcggatgcc gccctgcaag atgctgaaaa cgtctcttac gagatgctcg
    15781 tggcctccgc ctacaagtgg accatgaagg aaggcctggt tttcgacatc gaaaccgccg
    15841 acaacggcgc tgccgtcacc gaaggtttca ccgtccgcgt tgccctgaac cagcgctgca
    15901 gcccgcacga cacggcgacc aacccggcca gcccgcccac caacgtcggc gtttgcgctc
    15961 cgaccggcga tggcgcccag ttcgacgcca gcattccgat cgtcgcgggc gacctggtgc
    16021 tcgacccgac cttggccttg gagtggaagg ttacgttcga tggctacacc agcggtaaca
    16081 ccgttgcgac gttccgcatc gagccgctcg tcaccaaccc gactggcccg ggcaccaacg
    16141 gcaccaccat caccttcacc tggaacaacg cgcacctgac cagcctgaac gagttcgcgg
    16201 ccaccgccgc tgccggcggg aatgccaagg tcgatgccga gttctggatg gtgaacgtct
    16261 cgaacgacgc ccgcttcgtc aactcgaccc gttcgcgcac catcctcgaa agccgcaacg
    16321 gcgtggttgc ctgcgcaacc ccgaatccgg cggaaatcga caagtacatc gatgtcgccg
    16381 acaacttcaa cgaagaccag atcccgaaga cccgcttcag ctgggacggc aagctgggtt
    16441 cggc
//
LOCUS       DAORUK010000032        15972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_3400_length_15972_cov_18.974493, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000032 DAORUK010000000
VERSION     DAORUK010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15972
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3400_length_15972_cov_18.974493"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1996)
                     /locus_tag="PK818_03545"
     CDS             complement(5..1996)
                     /locus_tag="PK818_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HQY81841.1"
                     /translation="MKLRPALLAIAIVATLGLSACKREDAATPGASAVPAGETADQFI
                     ARVNTEFRKSYSELTSAQWLSSTYINSDTEALAAKANERWLTQLNGWIEQSRRFEGQQ
                     MSPGTARAIKLLKLMTAMPAPKDPQKLEELTKLATRMEGMYGAGSYCTGEGDAKTCRD
                     IGQLSEVLATSRDYDAQLDAWQGWHTVSQPMRKDYVRFAELVNEGARDMGFADAGEMW
                     RSGYDMSPAELAAETDRLWDQVKPLYEQLHCYARGKLEATYGSDRGQVAGGMLPAHLM
                     GNLWQQDWGNLWDMMQPYPGAGSLDVNAALRAIAQADLTSERARPGQPGASAAQREVE
                     AERAAALATAKRMTERAQDFYVSLGMPKLPQGYWEKSQFIKPRDRDVVCHASAWDMDM
                     AGDVRTKMCIVPSEEELTTIYHELGHIYYDLAYNKQPPLFQGGAHDGFHEAIGDTIVL
                     SMTPKYLASVGLVGAQQQSDEALINAQMRMALAKVAFLPFGLMIDRWRWGVFDGSIKP
                     DGYNQAWWELKAKYQGVAPASARDETHFDPGAKYHVPGNTPYTRYFLSHVLQFQFYKS
                     LCDAAGHTGPLYECSFYGNQEAGKRYWAMLGKGASQPWQQTMKELTGGEKMDASAVLE
                     YFAPLQSWLKERNAGSNCGWNAAGAAAAAPAPAASAKAG"
     gene            complement(2090..2584)
                     /locus_tag="PK818_03550"
     CDS             complement(2090..2584)
                     /locus_tag="PK818_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81842.1"
                     /translation="MAGSVLRLADIGFGDAATLLHRYGLELQHVADGEPIPGSYWGEC
                     EAGIIGHVVHARGDTPVHSLLHEACHLIVLPPERRAGVHTDATESIDEENAVCVLQAL
                     LADALPGVGRGRMLADMDAWGYTFRLGSAAAYVERDADDAWAWLQARGFVDDARAPRI
                     PAGD"
     gene            complement(2586..2852)
                     /gene="minE"
                     /locus_tag="PK818_03555"
     CDS             complement(2586..2852)
                     /gene="minE"
                     /locus_tag="PK818_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HQY81843.1"
                     /translation="MSIFDFLKPKKTTAQTAKNRLQIIIAQERRSEGAPDYLPLMRRE
                     ILEVIRKYVNVDVDAVKVDVVKDGEHDVLDISVSLPERGSPAGA"
     gene            complement(2856..3668)
                     /gene="minD"
                     /locus_tag="PK818_03560"
     CDS             complement(2856..3668)
                     /gene="minD"
                     /locus_tag="PK818_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HQY81844.1"
                     /translation="MAEIIVVTSGKGGVGKTTSSASIATGLARRGKKVAVIDFDVGLR
                     NLDLIMGCERRVVYDFVNVVNDEANLKQALIKDKRFENLFVLAASQTRDKDALTTEGV
                     EKVLKDLAEDGFDYIVCDSPAGIEKGAFLAMYFADKAVVVVNPEVSSVRDSDRILGLL
                     ASKTRRAERGDGDVTAYLLLTRYSPQRVENGEMLSITDVEEVLGLKTIGVIPESGDVL
                     NASNKGEPVIMDAESNAGQAYDDAVARLMGEERPMRFTQLERKGFFSKLFGG"
     gene            complement(3699..4478)
                     /gene="minC"
                     /locus_tag="PK818_03565"
     CDS             complement(3699..4478)
                     /gene="minC"
                     /locus_tag="PK818_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017115518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HQY81845.1"
                     /translation="MSAAVDYAQAGELKFGQVGIANLRVRTLDVAQLVAEMRDRVQRA
                     PNLFARAAVVLDFGGLGKAPSFEEARTLLDGLREAGVLPVALAYGTREIEALSQQLGL
                     PLLAKFRAQYERAEADAAPAPRAAAAEPARARAEPPAAAAAKPVSPTPAPGQVQRSPV
                     RSGQQVYADNRDLTVLGAVGAGAEVIADGSIHIYGALRGRALAGAQGNEDARIFCREF
                     HAELVAVAGHYKVLEDIPQALRGKAVQAWLENGQIKLAALD"
     gene            complement(4475..5089)
                     /locus_tag="PK818_03570"
     CDS             complement(4475..5089)
                     /locus_tag="PK818_03570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY81846.1"
                     /translation="MSIAIRDVREHELDSVLALNNAAGPAILPLDAARLRRLFDSAEY
                     FRVAERDGAIAGFLIGFGAHAHHDSSNFAWFAQRHPDFFYIDRVAVASRRRGGGVGRA
                     LYADVQSYAELRYPQLACEVFLQPGTDHALLFHGSFGFREVGQHVMPNRGAGGQEMRA
                     AMLMKSMCSYPWVRETYGDALPEQPWLLHPRPVHAAPRATGTGT"
     gene            5222..6418
                     /locus_tag="PK818_03575"
     CDS             5222..6418
                     /locus_tag="PK818_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017171659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQY81847.1"
                     /translation="MLARLSHTRLLRYAGLFTWAMVGLPLLYSWVGQWRNPLPDENMA
                     HAMPWQGWACYLAFGAGYWWLTRGLGQRRQAMDYVLLAVTTLAAIGLGFFSETGLGSV
                     LLMVAACVLPWLLPLRLGVAWLLGAQLAVVPMFMLGLEFPLVEALMQSMLYAGFSGFV
                     FITSLVALQQSEAREEQRRLNAELRATRALLAESARVNERTRISRELHDLLGHHLTAL
                     SLNLEVAGHLSEGRVKEHVQQAHTLARLLLTDVREAVSQLRESGAIDLGAALRPLAEN
                     VPKLDIHMDIQNPLTLEDPERAHVLLRCAQEAITNAVRHAGARNLWLSAHGDGTQVVM
                     QVRDDGDGAEHLVPGNGLQGLRERLRQCGGQLEVATARGEGFRLTMILPVAPSADAGF
                     PQGVAA"
     gene            6415..7071
                     /locus_tag="PK818_03580"
     CDS             6415..7071
                     /locus_tag="PK818_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002807809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQY81848.1"
                     /translation="MIRPDPIRVFLVDDQTLVRQGIRSLLALAEGIEVVAEAADGRQA
                     VELIPQVKPDVVLMDMRMPAMSGLEALQAMGRAGTLPPTIILTTFDDDQLVLAGIKAG
                     AKGYLLKDVSLEQLVNAIQAVAAGGSLVQPAVTQRLLSGLEHMRNEFVSLDRPDPLTD
                     RETEILRLMASGFSNKEIANSLGVAEGTIKNHVSNILSKLGVRDRTRAVLKAFELQLV
                     "
     gene            7229..8290
                     /locus_tag="PK818_03585"
     CDS             7229..8290
                     /locus_tag="PK818_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQY81849.1"
                     /translation="MTRLLEILISLAIVLGLYLAVALVLPSKRHLVEKIETNRKLTIV
                     FDSLNSLRRFKDWNPLVLRDPRVQLSYSGPDSGVGARMDYVSMEDGLGKGAWEITESV
                     PRKKISYKIDNPERGSNKRTSFIFKPTGRNNRNVEITQTYDVDYGWDLLGRYSGMYVS
                     RHVGDDMKMGLGRIVSMLAAVPNVDYAVSGSKLTGFKVVDRAAEDLLVVTAGSVERGN
                     SQIQASITANSEWIRRVMDANGLEAVGPVRIVTTELGRETYNFDVVQPVRKQGGGALG
                     NVALQGPVKLEQVKAGKVATASYTGYMAELENARNALRAWTLTSGHEVTGRAYESYKS
                     GVANAFTENGEFDVYWPLK"
     gene            8621..8800
                     /locus_tag="PK818_03590"
     CDS             8621..8800
                     /locus_tag="PK818_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81850.1"
                     /translation="MKANVQKALSVVVAAIGLALLVMMVVVEGEPGAIPLALILAGVV
                     GYASGRRRGRSGNPQ"
     gene            complement(8829..9209)
                     /locus_tag="PK818_03595"
     CDS             complement(8829..9209)
                     /locus_tag="PK818_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81851.1"
                     /translation="MIRILATAIAVLFGIATLFAGGRVLLGSDPGYVVFRPLLVYNVL
                     MGLAYVAAGIIFWRSARAGRNAAAAIFLLNLAVLLAILVLYRTGGDVAVDSLRAMTLR
                     TVVWLLLCVAMSWVGRSRAAPTSG"
     gene            complement(9353..9604)
                     /locus_tag="PK818_03600"
     CDS             complement(9353..9604)
                     /locus_tag="PK818_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81852.1"
                     /translation="MHVVFLHFIAATIACLLVFVAGNRIAGTQQFSAPFGVVFIGVAC
                     AALAHFLSPWATPAIVLLYALSVLGEAIRDRRDLRGRER"
     gene            complement(9664..10020)
                     /locus_tag="PK818_03605"
     CDS             complement(9664..10020)
                     /locus_tag="PK818_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012277143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HQY81853.1"
                     /translation="MREIGLTILMLTLSNVFMTFAWYAHLKELSAKPWLVAALASWGI
                     ALFEYMLQVPANRIGYGTLSLGQLKILQEVITLSVFVPFAVYYMKEPLKLDYLWAGLC
                     ILGAVYFIFRADMAVA"
     gene            complement(10039..10491)
                     /locus_tag="PK818_03610"
     CDS             complement(10039..10491)
                     /locus_tag="PK818_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81854.1"
                     /translation="MPGMTVAEKVELTLIPVVGAAVWSLAAAAGASIGTGSLLLGSSV
                     LLLLQGLVRDLWLISRRNRDAHAGAGREALCMCVESTIGVTGVVTGLAVLGSALDATL
                     ALGPEAMGAIAVVVLAIGFAIKDLVFELRPFRIRRDKDHLNIVFRWKP"
     gene            complement(10610..10786)
                     /locus_tag="PK818_03615"
     CDS             complement(10610..10786)
                     /locus_tag="PK818_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81855.1"
                     /translation="MAEIVVQRQPGAYNARDLAAFVATCSDDVALYRAKSGTPLALGK
                     RALAEFHEDSRFGR"
     gene            complement(10853..11269)
                     /locus_tag="PK818_03620"
     CDS             complement(10853..11269)
                     /locus_tag="PK818_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HQY81856.1"
                     /translation="MREAPMQAIADLETLLARMQPVLNPGRYAFVALPSGCMLDPALV
                     VASIREPEGLSVICTEHVARDMDLPIAFIASWITLAVHSDLAAVGLTAAFSSALARAG
                     VSCNVVAGVHHDHLFVPVEQTLQAMEVLHGLSRPPG"
     gene            complement(11319..11609)
                     /locus_tag="PK818_03625"
     CDS             complement(11319..11609)
                     /locus_tag="PK818_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81857.1"
                     /translation="MPDAINRGYKLALATLAAELLVSLALVSNTLGIFAILAMPLIGI
                     AWLPASVLVLSDLRREPRPGLPGYLVGVASGLAGLACLGICLKWTVLSAFAR"
     gene            complement(11712..12908)
                     /gene="chrA"
                     /locus_tag="PK818_03630"
     CDS             complement(11712..12908)
                     /gene="chrA"
                     /locus_tag="PK818_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HQY81858.1"
                     /translation="MSLPGVFLRFLRFGALAWGGPVAQIGMIRQQLVDEEGWIDRDRF
                     NRVLAVYQVLPGPEAHELCVYFGTLARGRLGGILAGLGFMLPGFVLMLALSWLYVRYG
                     LRLEGWAGAMVAAQAAVAALIVRAIFRIGSHVLENRWLWMVATVAVLSQLANVHFAVT
                     LAAGGLVYLLAHENRNLIAAWLLAMSIALVAVKVWSEGGLTGLDALTSASEPGSAARA
                     GSMGAAALFWSGLKAGLLTFGGAYTVIPFLQRDAVSQGAWMSNGQFLDGLALSGLLPA
                     PLVIFATFVGYVGGGLAGSLAMTAGVFLPAFLFTLLGHDAMERAVQRPRVAEFLAGIT
                     AAVVGLITGTTLAILSGSIRDAHSLLVFSVALAALFLSKARLLVPAVVVVAALYGLVA
                     STVGTG"
     gene            complement(13119..13523)
                     /locus_tag="PK818_03635"
     CDS             complement(13119..13523)
                     /locus_tag="PK818_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81859.1"
                     /translation="MRLVMCMAILLAPGLVTAREPSPVVGAWDWNPTQGSCPETHTYD
                     ANGSASVRSGDEVLEKSYTVTSVAGGMYLVKTVVVATNGGRDCLGKTSTIGATSSVYI
                     QPINGGGYFTCASEDGMSCYGTARPRHSPVGS"
     gene            complement(13613..14017)
                     /locus_tag="PK818_03640"
     CDS             complement(13613..14017)
                     /locus_tag="PK818_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81860.1"
                     /translation="MSRIRRLAASGVIGVFLAFALLWCWVHLAANNPLPGLLLRSGFG
                     ADGTWLALTASDFLMNIIMCLPAAWALNSLGKDLRLNTLVSVVAFAASSSFLVGLPLH
                     EMSLRIGIQYSLLLASLPVAVWVFSRLRRRRA"
     gene            complement(14001..14300)
                     /locus_tag="PK818_03645"
     CDS             complement(14001..14300)
                     /locus_tag="PK818_03645"
                     /inference="COORDINATES: protein motif:HMM:NF022969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HQY81861.1"
                     /translation="MTGAWLTTITESPQEGFELAIVLSRRGVKYTQPDVDVLKALRPG
                     YASSADALTAASHVIAVNFQTIAAANGYWRKDAASRSGRTQVSGTLQDGSHVAHP"
     gene            complement(14428..15234)
                     /locus_tag="PK818_03650"
     CDS             complement(14428..15234)
                     /locus_tag="PK818_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81862.1"
                     /translation="MSFQESLDSLPIGAFFVAYTVVAATISESGYRLGNWWQERTPEE
                     REGPTGMIVGSLLALMAFLLATATGMAADRFDTRRGLVLAEANSVGTTYLRAGYLPEP
                     ASSEVRSLLREYVPLRIIGTADLADVRVRMARSAELQTKLWSIAEDLARTTPESEVLA
                     LFIASLNETIDLHETRVVAGLYARVPETIILLLFSGTILTLGMVGYNAGLTRRRSPVT
                     AFAMIVVLGAVITLVVDLDRPRDGFLKVSQQPLIDLQEQIGALPPANPPR"
     gene            complement(15241..15540)
                     /locus_tag="PK818_03655"
     CDS             complement(15241..15540)
                     /locus_tag="PK818_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQY81863.1"
                     /translation="MADLDAVADYIALENPVAASELVKRIFDHVEHLTDHPESGSRLK
                     ELTRSRYRQIVEPPCRVFYRYDGHKVFVLHVMRSERLLRKGRLTARAKQAKTYQG"
     gene            complement(15558..15815)
                     /locus_tag="PK818_03660"
     CDS             complement(15558..15815)
                     /locus_tag="PK818_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HQY81864.1"
                     /translation="MRTELVTTLKRQATELLSDIERDKEPILITQHGLPSAYLLDVET
                     YQLMQQRMAILEGIARGEQAVAEGRVATHAQAKKRLARWLK"
BASE COUNT         2687 a         5283 c         5449 g         2553 t
ORIGIN      
        1 cggctcagcc ggctttcgcc gacgccgccg gggcaggtgc cgcggcggcg gcgccggcag
       61 cattccagcc gcagttgctg cccgcgttgc gttccttcag ccaggactgc agcggtgcga
      121 agtattccag caccgccgag gcgtccatct tctcgccgcc ggtgagttcc ttcatggtct
      181 gctgccacgg ctggctggcg cccttgccga gcatcgccca gtagcgcttg ccggcttcct
      241 ggttgccgta gaagctgcat tcgtacagcg ggccggtgtg tccggcggcg tcgcacagcg
      301 acttgtagaa ctggaactgc agcacgtgcg acaggaagta acgggtgtac ggcgtgttgc
      361 ccggcacgtg gtacttcgcg cccgggtcga aatgggtttc atcgcgcgcg gaagccggcg
      421 ccacgccctg gtatttcgcc ttcagttccc accacgcctg gttgtagcca tccggcttga
      481 tcgagccgtc gaacacgccc cagcgccagc ggtcgatcat cagaccgaac ggcaggaacg
      541 cgaccttggc cagcgccatc cgcatctgcg cgttgatcag tgcctcgtcg ctctgctgct
      601 gcgcgccgac caggccgact gaagcgaggt acttcggcgt catcgacagc acgatggtgt
      661 cgccgatcgc ttcatggaag ccgtcgtggg cgccgccctg gaacagcggc ggctgcttgt
      721 tgtaggcgag gtcgtagtag atgtgcccga gctcgtggta gatcgtggtc agctcttcct
      781 cgctgggcac gatgcacatc ttcgtgcgca cgtcgccggc catgtccatg tcccaggcgc
      841 tggcgtggca gaccacgtcg cggtcacgcg gcttgatgaa ctggctcttc tcccagtagc
      901 cctgcggcag cttgggcatg cccagcgaga cgtagaagtc ctgcgcgcgc tcggtcatcc
      961 gcttcgcggt ggccagcgcc gcggcgcgtt cggcctcgac ctcgcgctgg gcggcggagg
     1021 cacccggctg gccgggcctg gcgcgttcgc tggtgaggtc ggcctgggcg atcgcgcgca
     1081 gggcggcatt cacgtcgagg ctgccggcgc ccgggtaggg ctgcatcatg tcccacaggt
     1141 tgccccagtc ctgctgccac aggttgccca tcaggtgcgc gggcagcatg ccgccggcga
     1201 cctggcccct gtcgctgccg taggtcgctt cgagcttgcc gcgcgcgtag cagtgcaact
     1261 gctcgtacag cggcttgacc tggtcccaca ggcgatcggt ttcggcggcg agttcggccg
     1321 ggctcatgtc gtagcctgac cgccacattt cgccggcatc ggcgaagccc atgtccctgg
     1381 cgccttcgtt gaccagttcg gcgaagcgga cgtagtcctt gcgcatcggc tggctgacgg
     1441 tgtgccaacc ctgccaggca tcgagctggg cgtcgtagtc gcggctggtg gcgagcactt
     1501 cgctgagctg gccgatgtcg cggcaggtct tggcatcgcc ctcgccggtg cagtagctgc
     1561 cggcgccgta catgccttcc atgcgggtgg cgagcttggt cagctcctcc agcttctgcg
     1621 gatccttcgg cgccggcatc gcggtcatca gcttcagcag cttgatcgcg cgcgcggtcc
     1681 cgggcgacat ctgctggcct tcgaagcgcc tggactgctc gatccagccg ttgagctggg
     1741 tcagccagcg ctcgttggcc ttggccgcca gcgcctcggt atcgctgttg atgtaggtgc
     1801 tcgacagcca ctgcgcagag gtgagctcgg agtagctctt gcggaattcg gtgttgacgc
     1861 gggcgatgaa ctggtcggcg gtctcccctg cagggaccgc ggacgcgccg ggggtggcgg
     1921 cgtcctcgcg cttgcaggcg gacaggccaa gggtggcgac gatggcgatg gcgagcaggg
     1981 cgggacgtag cttcacgtgg catacctcgg cggcgcaggg cgcaggaggc gcaaggctac
     2041 cgcgtccagc gccggccgca agtgggcgcg ggctcgggcg cgggcggcgc tagtcgccgg
     2101 cgggaatccg cggcgcgcgc gcgtcgtcga cgaatccgcg cgcctgcaac cacgcccagg
     2161 cgtcgtcggc gtcgcgttcg acgtaggcgg cggcggaacc gaggcggaac gtgtagcccc
     2221 aggcatccat gtcggcgagc atgcgcccgc ggccaacacc gggcagggca tcggccagca
     2281 gcgcctgcag cacgcagacc gcgttctctt catcgatgga ctcggtggcg tcggtgtgga
     2341 cgccggcgcg ccgttccggc ggcagcacga tcaggtggca ggcttcgtgc agcagcgaat
     2401 gcaccggcgt gtcaccgcgc gcatgcacga cgtggccgat gatgccggcc tcgcattcgc
     2461 cccagtagct gccggggatg ggctcgccat cggcaacgtg ctgcaactcc agcccgtagc
     2521 ggtggagcag ggtggcggca tcgccgaagc cgatgtccgc cagccgcagg acgctgccgg
     2581 ccaggtcagg cgcccgccgg gctgccgcgc tccggcagcg acaccgagat gtcgagcacg
     2641 tcgtgctcgc cgtccttgac cacgtccacc ttcaccgcat cgacgtcgac gttcacgtac
     2701 ttgcggatca cctcgaggat ttcccggcgc atcagcggca ggtagtcggg cgcgccctcc
     2761 gacctgcgtt cctgcgcgat gatgatctgc aggcggttct tcgcggtctg cgcggtggtc
     2821 ttcttcggct tgaggaaatc gaagatcgac atgcctcagc ccccgaacag cttgctgaag
     2881 aaacccttcc tctccagctg ggtgaagcgc atcggccgct cctcgcccat cagccgcgca
     2941 accgcgtcgt cgtaggcctg gccggcgttg gactcggcat ccatgatcac cggctcgccc
     3001 ttgttggagg cgttcagcac gtcgcccgat tcggggatca cgccgatcgt cttcagcccc
     3061 agcacttcct cgacgtcggt gatcgacagc atctcgccgt tctcgacccg ctgcgggctg
     3121 tagcgggtca gcagcaggta ggcggtgacg tcgccgtcgc cgcgctcggc gcgccgggtc
     3181 ttgctggcga gcaggccgag gatgcggtcg gagtcgcgca cgctggacac ttccgggttg
     3241 accaccacca ccgccttgtc ggcgaagtac atcgccagga acgcgccctt ctcgatgccg
     3301 gcgggggagt cgcagacgat gtagtcgaag ccgtcctcgg ccaggtcctt gagcaccttc
     3361 tcgacgccct cggtggtcag cgcgtccttg tcgcgggtct gcgacgcggc cagcacgaac
     3421 aggttctcga agcgcttgtc cttgatcagg gcctgcttga ggttggcctc gtcgttgacc
     3481 acgttcacga agtcgtaaac gacccggcgc tcgcagccca tgatcaggtc gaggttgcgc
     3541 aggccgacgt cgaagtcgat gaccgcgacc ttcttgccgc ggcgcgcaag cccggtggcg
     3601 atgctggcgc tggaggtggt cttgccgacg ccgcccttgc ccgatgtgac tacgatgatt
     3661 tccgccaagt tggcttctcc gttggtacgc aatgcgcgct agtcgagcgc ggcgagtttg
     3721 atttgcccgt tttccagcca ggcctgcacg gccttgccgc gcagcgcctg cgggatgtcc
     3781 tcgagcacct tgtagtgccc ggcgaccgcc accagctccg cgtggaactc gcggcagaag
     3841 atgcgggcgt cctcgttgcc ctgcgcgccg gccagcgcgc ggccgcgcag ggcgccgtag
     3901 atgtggatgc tgccgtcggc gatcacctcg gcgccggcgc cgaccgcgcc cagcacggtc
     3961 aggtcgcggt tgtccgcgta cacctgctgg ccggaacgca ccgggctgcg ctggacctgg
     4021 cccggcgctg gcgtcggtga caccggcttc gccgctgcgg ctgcgggcgg ttccgccctg
     4081 gcgcgcgcgg gttcggcggc ggcggcgcgc ggggccggcg cggcatcggc ttcggcgcgt
     4141 tcgtactgcg cgcggaactt ggccagcagc ggcaacccga gttgctgcga cagcgcttcg
     4201 atttcccggg tgccgtacgc cagcgccacc ggcagcacgc cggcctcgcg caggccgtcc
     4261 agcagggtgc gggcctcttc gaacgaaggc gccttgccca gcccgccgaa atcgagcacc
     4321 accgccgcgc gcgcgaacag gttgggcgcg cgctgcacgc ggtcgcgcat ctcggcgacg
     4381 agctgcgcca cgtccagcgt gcgcacgcgc aggttggcga tgccgacctg gccgaatttc
     4441 aactcgcctg cctgggcgta gtcgaccgcg gcgctcacgt gccggtgccc gtggcgcgcg
     4501 gcgcggcgtg caccgggcgc ggatgcagca gccacggttg ctccggcagc gcgtcgccgt
     4561 aggtctcgcg cacccacggg tagctgcaca tcgacttcat cagcatcgcc gcgcgcatct
     4621 cctgcccgcc ggcgccgcgg ttgggcatca cgtgctggcc gacctcgcgg aagccgaagc
     4681 tgccgtggaa cagcagggcg tggtcggtgc ccggctgcag gaacacttcg caggccagct
     4741 gcgggtagcg cagttcggcg tagctctgca cgtccgcgta cagggcgcgg ccgacgccgc
     4801 cgccacggcg gcggctggcc accgcgacgc ggtcgatgta gaagaaatcc ggatgccgct
     4861 gcgcgaacca ggcgaagttg ctgctgtcgt ggtgcgcgtg cgcgccgaag ccgatcagga
     4921 agccggcgat ggcgccatcg cgctcggcga cccggaagta ttccgcgctg tcgaacagcc
     4981 ggcgtagcct ggccgcgtcg aggggaagga tcgcaggtcc tgcggcgttg ttcagggcga
     5041 ggacggaatc cagctcgtgc tcgcgcacgt cgcggatggc gatcgacatt ccttggctcc
     5101 gtatggcttg ctgcaggctg ccgcgccagc gcgcgacctc ggcggattat cgcatgggcc
     5161 ggcaatcgcg gccatgacct tcggcgcggg cagggccggc gcggaggggt tataagaagg
     5221 catgctcgcc cgcctgtccc acacccgcct gctgcgctac gccggcctgt tcacctgggc
     5281 gatggtcggg ctgccgctgc tgtactcgtg ggtcggccag tggcgcaacc cgttgccgga
     5341 cgagaacatg gcgcacgcca tgccgtggca gggctgggcc tgctacctgg cgttcggagc
     5401 cggctactgg tggctgaccc gcggcctggg ccagcgccgg caggcgatgg actacgtgtt
     5461 gctggcggtg accacgctgg ccgcgatcgg cctgggcttc ttcagcgaaa ccggccttgg
     5521 cagcgtgctg ctgatggtgg cggcctgcgt attgccctgg ctgctgccgc tgcggctggg
     5581 cgtggcctgg ctgctgggcg cgcagctggc ggtggtgccg atgttcatgc tgggcctgga
     5641 gttcccgctg gtcgaggcgc tgatgcagtc catgctgtac gcgggcttct ccggcttcgt
     5701 cttcatcacc agccttgttg cattgcagca gtcggaagcg cgcgaagagc agcgccggct
     5761 gaatgcggag ttgcgcgcca cccgcgcact gctcgcggaa agcgcgcggg tcaatgagcg
     5821 cacccgcatc tcgcgcgagc tgcacgacct gctgggccac cacctgaccg cgctcagcct
     5881 gaacctggag gtcgccggcc acctgtccga agggcgggtc aaggagcacg tgcagcaggc
     5941 gcacacgctg gcgcgcctgc tgttgaccga cgtgcgcgag gcggtcagcc agctgcgcga
     6001 aagcggggcg atcgacctcg gcgccgcgtt gcgcccgctg gccgagaacg tccccaagct
     6061 ggacatccac atggacatcc agaatccgct gacgctcgag gacccggagc gcgcgcacgt
     6121 cctgctgcgc tgcgcgcagg aggcgatcac caacgcggtg cgccacgccg gtgcccgcaa
     6181 cctgtggctg agcgcgcatg gcgatggcac ccaggtggtg atgcaggtgc gcgatgacgg
     6241 cgacggcgcc gagcacttgg tgcccggcaa cggcctgcag gggctgcgtg agcgcctgcg
     6301 gcagtgcggt ggccagctgg aagtggcgac cgcacgcggc gagggcttcc gcctgacgat
     6361 gatcctgccg gtggcgccca gcgccgatgc cggctttccc caaggagtgg ctgcatgatt
     6421 cgacccgatc cgatcagggt gttcctggtg gacgaccaga ccctggttcg ccagggcatc
     6481 cgttcgttgc tggccctcgc cgaagggatc gaggtggtgg cggaggccgc ggacggccgc
     6541 caggccgtcg agctgatccc gcaggtgaag cccgacgtgg tcctgatgga catgcggatg
     6601 ccggcgatgt ccgggctcga ggcgctgcag gcgatgggcc gcgccggcac cctgccgccg
     6661 accatcatcc tgactacctt cgacgacgac caactggtgc tggccgggat caaggcaggc
     6721 gccaagggct acctgctcaa ggatgtctcc ctggaacagc tggtcaacgc catccaggcg
     6781 gtggcggcgg gcggctcgct ggtgcagccg gcggtgaccc agcgcctgct gtccgggctg
     6841 gaacacatgc gcaacgagtt cgtcagcctg gaccgcccgg atccgctgac cgaccgcgag
     6901 accgagatcc tgcgcctgat ggcgtccggc ttctccaaca aggagatcgc caattcgctg
     6961 ggggtggccg agggcacgat caagaaccac gtctccaaca tcctgtcgaa actcggggtg
     7021 cgggaccgca cccgggcggt gctcaaggcg ttcgagctgc aactggtcta gacggcccgc
     7081 ggcaggccgc ggggtgcgtt cgtgaccggg cgcaccaatt ggcgtctggg ggcgctgccg
     7141 accccctccc cgagcccccg gtcggtttgg tacgatggcg ggtcttcgcg gccgcatgcc
     7201 gcgatgcggc cttcccggag attcctgaat gactcgtttg ctcgagatcc tgatttccct
     7261 cgcgatcgtg ctgggcctgt acctggccgt ggccctggtg ctcccgtcca agcgccacct
     7321 ggtcgagaag atcgagacca accgcaagct gaccatcgtg ttcgatagtc tcaacagcct
     7381 gcgccggttc aaggactgga acccgctggt gctgcgcgac ccgcgcgtgc agctgagcta
     7441 ctccggcccg gattccggcg tcggtgcgcg catggactac gtgtccatgg aagacggcct
     7501 gggcaagggc gcctgggaga tcaccgaaag cgtgccgcgc aagaagatca gctacaagat
     7561 cgacaacccg gagcgcggca gcaacaagcg caccagcttc atcttcaagc cgaccggccg
     7621 caacaaccgt aacgtcgaga tcacccagac ctacgacgtc gactacggct gggacctgct
     7681 gggccgctac tccggcatgt acgtctcgcg ccacgtcggc gacgacatga agatgggcct
     7741 cggccgcatc gtcagcatgc tggcggcggt gccgaacgtc gactacgcgg tctccggcag
     7801 caagctgacc ggcttcaagg tcgtcgaccg cgcggccgag gacctgctgg tcgtgaccgc
     7861 cggcagcgtc gagcgcggca attcgcagat ccaggcctcg atcaccgcca acagcgagtg
     7921 gatccgccgg gtgatggacg ccaatggcct ggaggcggtt ggcccggtcc gcatcgtcac
     7981 caccgaactc ggtcgtgaaa cctacaactt cgacgtcgtc cagccggtgc gcaagcaggg
     8041 tggcggcgcg ctcggcaacg tggcgctgca gggtccggtg aagctggagc aggtcaaggc
     8101 cggcaaggtc gccaccgcgt cctacaccgg ctacatggcc gaactggaga atgcccgcaa
     8161 cgcgctgcgc gcatggaccc tgacctccgg ccatgaagtg accggccgcg cctacgagtc
     8221 ctacaagtcg ggcgtcgcca atgccttcac cgaaaacggc gagttcgacg tctactggcc
     8281 gctgaagtga tcgcaccggc ctaggttcgc cgatgccaac gcagtaaacg aacgccgcgc
     8341 ccgtcgcggc gttcgttttt gcggggcggg ttggggtcgc gcagccgcgc tgcggccccg
     8401 ggtcctggcg tccatgccgc gcgtgtgcac ctgggccggc gcgagcgcac gctggaggcg
     8461 cagccgcggc ggccgcgccc gatcgagacc ggccacgcac gcacatgtgc gcgactgatc
     8521 ggcccggccc cgcgcgcacg ggacttcatg ccggcctcac gccgcggtgc cagattgtcg
     8581 tcatcgaggc gggtcggtcc gcgatcgaca ggggcacgca atgaaggcga acgtgcagaa
     8641 ggcgctgtca gtggtggtgg ccgcgatcgg gctggcgttg ctggtgatga tggtggtcgt
     8701 ggaaggcgaa ccgggtgcga tcccgctggc actgatcctg gccggagtgg tcggctacgc
     8761 cagtggccgc cgcaggggca ggtccggcaa cccgcagtga gcccgcgcag ggcgggcagc
     8821 ggcgtgcttc agccggacgt gggtgccgcg cgtgaccgtc ccacccagga catggccacg
     8881 cacagcagca gccacaccac cgtgcgcagg gtcatcgcgc gcaggctgtc caccgccacg
     8941 tcgccgccgg tgcggtacag caccaggatt gccagcagca ccgccaggtt gagcaggaag
     9001 atcgcggccg ccgcgttccg gcccgcgcgc gcgctgcgcc agaagatgat gccggcagcc
     9061 acgtaggcca ggcccatcag cacgttgtag accagcagcg gccggaacac gacgtagccc
     9121 ggatccgacc ccagcagcac gcggccaccc gcgaacaggg tcgcgatgcc gaacaggacc
     9181 gcgatggcgg ttgcgaggat ccggatcatg cgaactcctt gatgggcgcc gatgcggggc
     9241 gtggcggccg ccacgtgtgt tccgggtagc cgccagaccc gatcgtagcg gggtcctgcc
     9301 ggcgctgcat gcaactgcag gttgcagcgg ctggcgcgcg gcatcttccc tgctagcgct
     9361 cgcggccgcg caggtcccgg cgatcgcgga tcgcctcgcc cagcaccgaa agcgcataca
     9421 gcagcacgat ggccggggtt gcccatggcg acaggaagtg ggcgagggcg gcgcacgcca
     9481 ccccgatgaa caccacgccg aatggcgccg agaactgctg cgtgcccgcg atgcggtttc
     9541 cggccacgaa caccagcagg cacgcgatcg ttgcggcgat gaagtgcagg aagacaacgt
     9601 gcatgcgcac ctcgcgttgc ggccccgggc gccggggcct ggcggacaga tcccttgggc
     9661 aaatcaggca accgccatgt ccgcacggaa gatgaagtag accgcaccca ggatgcacag
     9721 gcccgcccac aggtagtcca gcttgagcgg ttccttcatg tagtaaacgg cgaaaggcac
     9781 gaacacggac agcgtgatca cctcctgcag gatcttgagc tgcccgagcg agagcgtgcc
     9841 gtagccgatg cggttcgccg ggacctgcaa catgtactcg aagagcgcga tgccccagct
     9901 ggccagcgcc gcgacgagcc acggctttgc actcagttcc ttgagatggg cgtaccacgc
     9961 gaacgtcatg aagacgttgc tcagcgtcag catcaggatg gtcaggccga tttcgcgcat
    10021 cagtgcatcc ggtgccggtc agggtttcca gcggaaaacg atgttcaggt ggtccttgtc
    10081 acggcggatg cggaagggcc ggagctcgaa caccagatcc ttgatcgcga acccgatggc
    10141 caggaccacg accgcgatcg cgcccatcgc ctccggcccc agggccagcg tggcgtccag
    10201 tgcgctgccg agcaccgcca gcccggtgac gaccccggtc acgccgatcg tggattccac
    10261 gcacatgcac agtgcttcgc ggccagcacc cgcatgcgca tcccggttac gcctggaaat
    10321 gagccagagg tcgcggacca gcccctggag cagcagcagg acggaggatc caagcaacag
    10381 cgagccggta ccgatgctgg ccccggccgc cgctgcaagc gaccacaccg ccgcgccgac
    10441 caccgggatc agcgtgagtt ccactttttc cgcgacggtc atgccgggca tgggcctggc
    10501 gcctccattc ttgttgcttc ggacgcctgc attctagtcg gcatgaaaca gccaaacgcg
    10561 ctggatgagg ccatcctcga gcacgcgggc gacgaccgct tcgacacgct catcgcccaa
    10621 accgcgagtc ctcgtggaac tcggcgagcg cccgcttgcc caatgccagc ggcgtgcctg
    10681 atttcgcccg gtacagcgcc acgtcgtcgc tgcatgtcgc cacgaacgcc gccaggtcgc
    10741 gcgcgttgta ggcaccaggc tggcgctgga ccacgatttc ggccacgggg aacggcgggc
    10801 aatcccggcg tcgatgccgg ggccgccagc acgagacggc caggcagaag ggtcaccctg
    10861 gaggtcgcga caacccatga aggacctcca tcgcctgcag tgtctgctcc accggcacga
    10921 acaggtggtc gtggtggacg ccggcaacca cgttgcaact gaccccggcc cgtgcaagcg
    10981 cactggagaa ggcggcggtc aggccaaccg ccgcaagatc cgaatgcacg gccagcgtga
    11041 tccaggacgc aatgaaggcg atcgggagat ccatgtcgcg ggccacatgc tccgtgcaga
    11101 tcaccgagag tccttccggc tcccggatcg acgccaccac cagcgcgggg tcaagcatgc
    11161 agccactggg caaggcgacg aacgcatagc ggccgggatt gagcaccggc tgcatgcgcg
    11221 ccagcaatgt ttcgaggtcg gcgatcgcct gcattggtgc ttctcgcatg ggggaagggt
    11281 ggcgcggggt ggagtcggct agaacgaacc gccgggcgtc acctcgcgaa cgccgacagg
    11341 accgtccatt tgaggcagat gcccaggcag gcaaggcccg caaggccgct tgcaacgcca
    11401 accaggtagc caggcaggcc aggacgaggc tcccggcgca ggtcggacaa gaccagcacc
    11461 gaagcaggca accaggcgat gccgatcaag ggcatggcga ggatggcgaa gattccaagc
    11521 gtgttcgaaa ccagggcaag cgagaccagg agctcggcgg cgagcgtcgc cagggcgagc
    11581 ttgtaacctc gattgatggc gtcgggcatg ggaggcttgt cacccgggtt gggccggacc
    11641 gaacgaactg tgggcgtcgc cgccctttat gccggcggcg cgtgaatcca gcaactcgcg
    11701 cgcttgcagg atcaaccggt cccgactgtg ctggcgacca gcccgtacag tgctgccacc
    11761 acgaccactg ccggtaccag caagcgtgcc ttcgacagga acagggccgc aagtgccacc
    11821 gagaacacca gcaggctgtg cgcatcccgg atgctgccgg agaggatggc aagggtcgtg
    11881 cccgtgatga ggccgacgac agctgcggtg atccccgcca ggaactctgc gacccggggc
    11941 ctttgcaccg cgcgctccat cgcgtcgtgg ccgagaagcg tgaacaggaa cgcaggaagg
    12001 aatacgccgg ccgtcattgc cagggaaccc gcgagtccgc cacccacata accgacgaag
    12061 gtggcgaaga tgaccagggg cgcgggcagc aggccggaca gtgccaggcc atccaggaac
    12121 tggccattgc tcatccacgc gccctggctc acggcatcgc gttgcaggaa tgggatgacg
    12181 gtataggccc cgccgaacgt cagcaggcct gccttgaggc ccgaccagaa gagtgcggca
    12241 gcgcccatgc tcccggcccg tgccgcactg cctggctccg aggcgctggt gagggcatcc
    12301 aggccagtga ggccgccctc gctccacacc ttgaccgcga ccagggcgat ggacatggcc
    12361 agcaaccatg cggcgatcag atttcgattc tcgtgcgcaa gcaggtagac caggccccct
    12421 gcggcgagcg tcacggcgaa gtgcacgttt gccagttgcg acagcaccgc gaccgtggcg
    12481 accatccata accagcgatt ttcgaggacg tgggaaccga tgcgaaaaat ggcgcgaacg
    12541 atcagtgccg cgaccgctgc ttgtgctgca accatcgcac cggcccagcc ctcgagcctc
    12601 aatccgtaac gcacgtacag ccacgacagc gccagcatca gcacgaatcc cggaagcatg
    12661 aagcccagtc ccgccaggat tccgcccagg cgtccgcgag cgagcgtccc gaaatagacg
    12721 cagagttcat gcgcttccgg ccccggcagg acctggtaga ccgcaagtac gcgattgaac
    12781 ctgtcccggt cgatccagcc ctcttcatcg accagttgct gccggatcat gccgatctgc
    12841 gccacaggac cgccccaggc cagggcgccg aagcgcagga agcgcaggaa tacaccgggc
    12901 agggacaggt gcggagttcc gcggcctgga tggactcctg gctgcttgtc cgctggcgag
    12961 tcagggtgca cggcctttct ccaatcgcgc aggactgcgc attcgttcgg aatcaggccg
    13021 ctcgggcaaa tccccggcaa gcctggcgac gcggtcgggc cggaccgccc gcggaggccc
    13081 ggcgaacgcc gcgaagcggg attggcgtgg acaaagggtc aggagccaac cggggagtgc
    13141 cgtgggcggg ccgtcccgta gcaggacatg ccatcttccg aagcgcaggt gaagtagcca
    13201 ccgccgttga tgggttgtat gtagaccgac gaggttgctc caatggtgct ggtcttgccc
    13261 aggcagtcgc ggccgccgtt ggtcgcgaca accaccgtct tgaccaggta catgccgcca
    13321 gcgaccgagg tgaccgtgta gctcttttcc aggacctcgt cgccactgcg gacgctcgca
    13381 cttccattgg cgtcgtaggt atgggtttca gggcagctgc cctgcgtcgg gttccagtcc
    13441 cacgcgccga caaccgggct gggttcccgg gccgtcacca ggcccggagc gagaagaatg
    13501 gccatgcaca tcacgagacg cataagtggt tccttcagcg tggggcagtc cggaccgcag
    13561 ccggcgagca aggcacgcgc gaccgcgaaa cggcttcgga ccggacgcct cgtcaggcgc
    13621 gccggcgacg aagccttgaa aagacccaga cggcgacggg cagtgatgcg agcagcagcg
    13681 agtactggat gccgattctc aagctcatct catgcaacgg caagcccacc aggaacgagc
    13741 tgcttgctgc gaaggcgacg acggaaacca gcgtgttgag ccgcaggtcc ttgcccaatg
    13801 aattcagcgc ccaggccgcg ggcaggcaca tgatgatgtt catcaggaaa tcgcttgccg
    13861 tcagggcaag ccaggtgcca tccgcgccaa agccactccg aagcagcagc cccggcagcg
    13921 gattatttgc agcaagatgg acccagcacc aaagcagggc aaacgcgaga aacacgccga
    13981 tcacgccgga tgctgcgagc ctacggatgc gcgacatgcg aaccgtcctg gagagtgcct
    14041 gacacctggg tccggccgga ccgcgaagcg gcgtccttgc gccaataacc gtttgccgca
    14101 gcgatggtct gaaagttgac agcgatgaca tgcgacgctg cggtgagcgc atccgcagag
    14161 ctcgcgtaac cgggacgaag cgccttcagc acgtccacat ctggttgtgt gtacttgacg
    14221 ccacgccggc tgaggacaat ggccagctcg aatccttctt gcggactctc agtgatggtc
    14281 gtgagccaag cacctgtcat gactggattc tttggtgagc atctgaagca aaagactaca
    14341 gcgccgcctg acgaccaagc tcagccgcgg acagccctcg aagcgggttg gccatcgacc
    14401 ggagcgattt gtcgggcggc acgccggcta ccttggtgga ttcgctggtg gaagtgctcc
    14461 gatttgctct tgcagatcga taagcggttg ctggctcact ttgaggaatc cgtcgcgtgg
    14521 tctgtcgaga tcgaccacga gtgtgataac cgccccaagc acaacaatca tcgcgaatgc
    14581 tgtgacgggg ctgcgccgac gagtcaggcc cgcgttgtat cccaccatcc ccaacgtcag
    14641 gatcgttccc gaaaagagga ggaggattat cgtctcgggt acgcgcgcgt acaggcctgc
    14701 cacgaccctc gtctcatgca ggtcgatcgt ctcgttcagt gaagcgatga aaagggccag
    14761 gacctccgat tcgggcgtgg tgcgggccaa gtcctcggcg attgaccaga gcttcgtctg
    14821 gagttcggcc gaacgggcca ttctcacccg gacatctgcc agatcggcgg tcccgatgat
    14881 gcgcaggggg acatactcgc ggagcaggct ccggacctcg ctcgaggccg gctctggcag
    14941 gtagccggcc cggagatacg tggtgcctac cgagttcgcc tcggccagca cgagaccgcg
    15001 gcgcgtgtcg aagcgatctg ccgccatccc cgtcgcggtc gcgagaagaa acgccatcag
    15061 ggctaggagt gagccgacga tcatgccggt gggaccctcc ctttcttcgg gcgtgcgttc
    15121 ttgccaccag ttcccaagtc ggtaaccaga ctccgagata gttgccgcga cgaccgtata
    15181 cgccacaaaa aaggcgccga tcggcagtga atcaagtgat tcttggaagc tcatcgtgcc
    15241 tcatccttgg taggtctttg cttgcttggc acgcgcggtg agtcgtcctt tgcgcagcag
    15301 gcgctctgaa cgcatgacgt gcaggacaaa gaccttgtgt ccgtcgtaac gatagaaaac
    15361 acggcagggc ggctcaacta tctggcgata gcgggagcgc gtgagttctt taaggcgact
    15421 gccactctcc gggtggtcgg tgaggtgctc aacgtgatcg aagatccgtt tgacaagctc
    15481 ggaggcagca actggattct ccagagcgat gtaatcagcg actgcatcca ggtcagccaa
    15541 ggccggttct gaccatgcta tttcagccat ctggcgagac gcttcttggc ttgcgcatgg
    15601 gtagctacac gaccctccgc cacagcctgc tcgccacggg cgatgccttc aaggatggcc
    15661 atgcgttgct gcatgagctg ataggtctca acgtccagga ggtaggcgct tgggaggcca
    15721 tgctgggtaa taagaattgg ctccttgtcc cgctcaatgt ccgagaggag ttcagtggcc
    15781 tgacgcttga gggtagtgac gagttcagta cgcatagagt gacactacag tatcactttg
    15841 gcgcgcgcaa ctggtggtgc aaagacctaa cgcctgagtt gagccgggcc gcagcgcgga
    15901 acggcggagt ggtacacgtc accatgcagc cgagccgcga agcgggctcg gcttgaacga
    15961 attgttaggt gc
//
LOCUS       DAORUK010000033        15869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_3429_length_15869_cov_17.429746, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000033 DAORUK010000000
VERSION     DAORUK010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15869
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3429_length_15869_cov_17.429746"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PK818_03665"
     CDS             complement(<1..358)
                     /locus_tag="PK818_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY81865.1"
                     /translation="MRKSNVVIIGASSGLGAGMAREFARRGHDLALCARRLDRLQALK
                     EELEAAHPGVRVLVEVLDVTDHARVFEVVRGFRAAFGELDRVVVNAGIGQGMPVGKGG
                     FERNKAIIETNLIAALA"
     gene            complement(406..1098)
                     /locus_tag="PK818_03670"
     CDS             complement(406..1098)
                     /locus_tag="PK818_03670"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQY81866.1"
                     /translation="MSAVYLVRHGQASFGAADYDVLSERGQQQAQVLGQALARAQAGF
                     AVVASGDLQRHRQTAQACLEAMGLAPAWRSDAGWNEFDHVRVITALRPDFADPERARA
                     EMMAQPDPQRAFQAMFEQAVARWVGGAHDADYSESWAAFRARCMQALGSVLDALPGGS
                     DALVFTSGGPIAAVAQHLLRIPDSDGFRLSWALVNCGVTKLVRGREGVSLSTLNGHAH
                     FEGRHAGLVTYR"
     gene            complement(1095..2171)
                     /locus_tag="PK818_03675"
     CDS             complement(1095..2171)
                     /locus_tag="PK818_03675"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HQY81867.1"
                     /translation="MSVAPADEARAVRSGEELDVAAVDAWLKGALPALQGTPTVTQYP
                     GGASNWTYRLQYANDDLILRRPPAGTKAKSAHDMGREYRIQQLLKPVFPLVPEMRALC
                     TDPAVIGTDFYVMQRLAGVIPRKDLPRGVELSPAQVKQLCLNVLDTLVALHQVDHRAA
                     GLEGIGKGAGYAQRQIEGWSRRYRDARTWNVPRGKRIMAWLAANLPAQERICVTHNDF
                     RFDNAVLDPDDPTRVVGVLDWELATLGDPLMDLGNTLAYWVEAGDDVMAQGMRRQPTH
                     LPGMLTRREVIDHYCGRMGFDPGDVTFHEVYGLFRLSAIAQQIYFRYHHGQTRNPAFK
                     RFWLFVHYLHWRCRRAIARQRVAA"
     gene            complement(2168..3046)
                     /locus_tag="PK818_03680"
     CDS             complement(2168..3046)
                     /locus_tag="PK818_03680"
                     /inference="COORDINATES: protein motif:HMM:NF004196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY81868.1"
                     /translation="MADPAMDQASIADNGKRIFITGGASGLGRALAERHARAGWRVCI
                     GDLDMERCAGALAAIRVHSPSSHALACDVTREDALQAAADWLQREWGGVDVVVNNAGV
                     AQMGGIAETSLEDWRWAVDINLLGVVRGCKVFVPLLRARDAGGRGGQLVNIASMAGLV
                     HMPQASAYNATKAAVVALSETLQLELEADGIRVSVVCPAFFRTGLAGNMRAANPALEG
                     MTKRLVERARIGADEIADRIFDGMARGDPHILTHPEARRFWRMKRLLPYRLYLALMRR
                     QLSRVAARMRRKAASR"
     gene            complement(3039..4265)
                     /locus_tag="PK818_03685"
     CDS             complement(3039..4265)
                     /locus_tag="PK818_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY81869.1"
                     /translation="MDFAPSAKTRDYLQRMQAFIAEHVAPREQEYRRANAANAGADWN
                     AWQVPAPMDEWKSRARAAGLWNLFLPDAALGAGLSTLEYAPLAEVMGRNEFLAEIFNC
                     SAPDTGNMEVLYHYGSEAQKARWLTPLLAGEIRSVFCMTEPDVASSDATNMQATIRED
                     GDELVLDGRKWWSTGIGHPRCRLAIFMGVSDPDAEPHARHAMVLVPMDTPGVRIQRML
                     PVHGEYDAPHGHGEVLFDGVRVPRDNLLLGRGKGFAIAQGRLGPGRIHHCMRAIGAAE
                     HALELTIRRGLERVAFGKPILRLGGNLERVADARIAIDQARLLTLYAAWKIDAVGVKQ
                     AMTEISAIKVVAPGMLQAVVDQAIQVHGGAGVSNDLPLAGLFAIARALRFADGPDEVH
                     RAMVARMELAKYKPRG"
     gene            complement(4265..5272)
                     /locus_tag="PK818_03690"
     CDS             complement(4265..5272)
                     /locus_tag="PK818_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HQY81870.1"
                     /translation="MNATSNRQFRLAARPVGLPRASDWSLVEEPVREPGEGEFLVRIE
                     YVSLDPAMRGWMNEGKSYVPPVAIGEVMRAYSAGEVIASNNPRFPVGSRVAGVFGVQQ
                     YVVSDGKGARLVDTSLAPLPTYLSVLGMTGMTAYFGLLEVGAAKQGDTVVVSGAAGAV
                     GMVVGQVARIKGCRVVGIAGGADKCRYLVDELGFDAAIDYKREDVRAALRQHCPKGID
                     VYFDNVGGEILDIALGQLAMHARVVICGAISQYNTTEGVKGPANYLSLLVNRARMQGM
                     VVFDYASRYGEAAQDMAGWLADGRLKSREDIVEGFDTFPDTLLRLFRGENTGKLLLKV
                     G"
     gene            5353..6282
                     /locus_tag="PK818_03695"
     CDS             5353..6282
                     /locus_tag="PK818_03695"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQY81871.1"
                     /translation="MNIIHSNAGQLDLNLLKVFEAAYREQHLGRAAQSLHLTPSAVSH
                     ALRRLRAQLGDPLFVRDGRRMLPTPACQRLAPPLLETLAQLRQWLQQLQHFDPAQARQ
                     AFHVGMPYAIEAMLLPAIYRLLRERAPRATLASVGYERRDLARALAAGQLDLAIDVAL
                     PASEPVRHAVLLQQGFCIAVRSRHALKRRPTLPQYLQAPHVAVSSRASGAVVEDIALL
                     NLGLQRRIALRCQDYQAACRVVAGSDHLLTLPTGLARQMAGRSLRLLPLPFELPPIQP
                     HLYWHANLAVDPANQWLRQLVVDACRTLDGGTG"
     gene            complement(6279..7490)
                     /gene="fabB"
                     /locus_tag="PK818_03700"
     CDS             complement(6279..7490)
                     /gene="fabB"
                     /locus_tag="PK818_03700"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015472729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HQY81872.1"
                     /translation="MRRVVVTGMGIVSSLGNGLESVSAALRAGRSGIRQIPEYAGLGL
                     RSQVAGVPEIDLDASIDRKQKRFMGDAAAYAMVAMRDAIADAGLLPSQVSQPRTGVIA
                     GSGGGSPQWQVETADLLRQKGVRKVGPYMVPRTMCSTVSAALATVFAIKGVSYSLSAA
                     CATSAHCIGAAADMIRHGMQDIMFAGGGEEAHWSMTCQFDAMGALSSHFNDTPEIASR
                     PYDADRDGFVIAAGGGMLVLEEYEHAVARGARIHGELLGYGVTSDGADMVAPSGEGAV
                     RCMQMAMEGLDRPIDYLNTHGTSTPLGDVTELQAVRAAFGAGNEPPLSSTKALSGHSL
                     GAASVHEAIYSLLMLRDRFIAGSANIARLDPACEGFPIVRESRAADLLTVMSNSFGFG
                     GTNATLVFGRT"
     gene            complement(7496..8011)
                     /gene="fabA"
                     /locus_tag="PK818_03705"
     CDS             complement(7496..8011)
                     /gene="fabA"
                     /locus_tag="PK818_03705"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017914959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HQY81873.1"
                     /translation="MTRPSSLDREQLLACARGEMFGPGNARLPAPPMLMFDRITSITE
                     DGGAHGKGFIRAELDINPDLWFFGCHFLGDPVMPGCLGVDAMWQLTGFFLPWLGEPGR
                     GRALGAGEIKFTDQVLPDAKVVSYEIDIRRVLRGRLKLVIADGRTYVDGREIYTAADM
                     RVGLFQSTEAK"
     gene            8181..9650
                     /gene="zwf"
                     /locus_tag="PK818_03710"
     CDS             8181..9650
                     /gene="zwf"
                     /locus_tag="PK818_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HQY81874.1"
                     /translation="MTRPAPASLLTIFGATGDLAQRMLLPSLYSLQAENLLPPGLRIL
                     GSARSDLDREAFANLVADAINERIPAVERDDTALRGLLERLDYAAASVDDAASMQALA
                     ARVSDLRNGDVLYHLSTAPRFYVPACHALGAHGAADARTRVMLEKPIGKDLASAAAIN
                     DGVAEVFDEDRVYRVDHYLGKEGVQNLIALRFGNALFEPLWNARHIEQVQITAGETVG
                     VEGRGDYYDDSGAMRDMLQNHLLQLLCLVAMEPPARFEPTAVRNEKIKVLRSLRAIGH
                     GQVATESVAGQYTAGAIDGVAVPGYVQELGRDSGTETFVALRAHVDNWRWSGVPFYLR
                     TGKRLPSRCTEIYIQFRKVPYSIFGGHAAADMRSNGLLIRLQPEERIELDLMAKTPGL
                     DRSGLRLSQVALDLDFHEEFANQRRRISYERLYLDAIEGNGTLFVRRDETETAWHWVD
                     AILDGWRTAGVAPKPYPAGTWGPGAAVSLIERHGHGWRE"
     gene            9650..10348
                     /gene="pgl"
                     /locus_tag="PK818_03715"
     CDS             9650..10348
                     /gene="pgl"
                     /locus_tag="PK818_03715"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HQY81875.1"
                     /translation="MGWIEHDHPSTDALVEQVAQQLQHACREAIHARGAAWLALAGGR
                     TPLPAYARLAASEFEGSITAVPTDERCVPHDHPACNLRALREAFAANRGITTAGITRE
                     DGDEGASLAQARALLALNAQPFDLVLLGMGTDGHFASLFPGAANLAEGLAMESGIDAI
                     ATLPEPLPAEAPFARISLTLPRLLRARTILLVATGEDKRRVLRQARQQDRGGHPVAAL
                     LHARDHRVQVHWSP"
     gene            10348..12180
                     /gene="edd"
                     /locus_tag="PK818_03720"
     CDS             10348..12180
                     /gene="edd"
                     /locus_tag="PK818_03720"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HQY81876.1"
                     /translation="MPHLHPVVERVTARIAERSRRLRGDYLARMDAARLDGPARRELG
                     CGNIAHAFAAAGADKAQLRNGSGGNLGIVTAYNDMLSAHQPMEQYPTLIRMAARNAGG
                     SAQVAGGVPAMCDGVTQGRAGMELSLFSRDAIAMATAISLSHAMFDGALMLGVCDKIV
                     PGLLIGALGFGHLPAIFVPAGPMPSGIPNRQKAEVRQRYALGKASREELLDAEAASYH
                     APGTCTFYGTANSNQMLMEVMGLHMPGSAFVAPGTPLRDALTVAATEQALRISALGSD
                     YRPLARTVDEKAIVNAMVGLAATGGSTNHALHLVAIARAAGLVIDWDDLDELSKATPL
                     LARVYPNGAADVNHFHAAGGLGFVIRELIDAGLLHPDIACVHGGDLRQQALEPFLDGI
                     TLRWREPPARSLDASVLRGVAAPFDSEGGLRRLQGNLGRATVKISAVAPEFRRIEAPA
                     RIFESQDALLDAFKADQLDGDFVAVVRGQGPRANGMPELHKLTPTLAALQDRGQRVAL
                     LTDGRMSGASGKVLAAIHVTPEAACSGPLAKLRDGDPVRIDAEAGTMEALVEAGAWAA
                     RACAKPALAGNHLGHGRELFGLMRRQVGAAEQGACSLFVDEDGE"
     gene            12177..13184
                     /gene="glk"
                     /locus_tag="PK818_03725"
     CDS             12177..13184
                     /gene="glk"
                     /locus_tag="PK818_03725"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HQY81877.1"
                     /translation="MTTLALVGDIGGTNARFALTDLAAPAIELQQAQSLRNADFASVQ
                     HAVEHYLQAIDARPKHAALAVACPVGSDEIRLTNRAWSFSRRELQRTLQLEQLRMIND
                     FGAVAWAVPSLERDNLVTLHGDPAAPLRGPVSVLGPGTGLGVALLVGSREHGWHAVET
                     EGGHTSFAPIGDEERAISAWLTAQHGRTSTERLLCGKGLSEIDAVLRGASAVLPSGLP
                     VPGDTSLLKPALRDPAEIVAAALEGHDLAARQTLARFCAVLGSVAGDCALIQGARTVV
                     IAGGIVPRFIPFLRSSAFRERFLAKGRMATLLESVPIHVITHPQPGLLGAATALRSSE
                     L"
     gene            13192..13839
                     /locus_tag="PK818_03730"
     CDS             13192..13839
                     /locus_tag="PK818_03730"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HQY81878.1"
                     /translation="MTDTASHASQVDAVFARAPVIPVLTIERIEDAIPLARALSDNGL
                     PVLEVTLRSACAVDAIALIARELADACVGAGTVLNARDLDAVAQAGARFAISPGATDS
                     LYRAAAECPVPLIPGIATASELMRGLEHGWQRFKFFPAESSGGIGALKGFAGPFAQVR
                     FCPTGGIDAAKAPAYLGLPNVACVGGSWMLPADALAARDWARIGGLARAAAALTR"
     gene            complement(13891..15309)
                     /locus_tag="PK818_03735"
     CDS             complement(13891..15309)
                     /locus_tag="PK818_03735"
                     /inference="COORDINATES: protein motif:HMM:NF007790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HQY81879.1"
                     /translation="MDTNMVQLAVFLGLTALVALATWWRYGKEAKAGDSAQDFFLAGK
                     HLSWPFIAGSLLLTNISAEQIVGMNGAQNMLQVWWEFGAAAGLLVLAKVLVPMYYKYD
                     CTTTTELLEKRLPNGASLRRTVSVLFLLGYMFILLPMVLYTGSRFMKSMFEPDMSILT
                     ISILFALAGLAYAALGGLRAIAISDTYMGVILLVMGGLVTWFALDRIGWDFSGIPAER
                     LTVWGGPDSDIPWPTLMTGMVFAHIFYWGTNMLIAQRALAAKDIREAQKGIYAAAALK
                     FLTPFIVILPGIIAFKLYGDIDDNAYGRLVGDVLPPWLSGAFAAAITGAVLSSYNAGL
                     NSAAALYTVDLHLPMVNPKADGKRIGQVVQLVFMLVSLAMVPLYQNATSIIGTLQQLN
                     GLYSMPVLASFIVAIFVRGANATAIRIALVFGVALYAMFTFAWTPLHYLHLMFITLAA
                     TILVAVVLGRVMGAPEPPSPAV"
     gene            complement(15414..>15869)
                     /locus_tag="PK818_03740"
     CDS             complement(15414..>15869)
                     /locus_tag="PK818_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012479380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY81880.1"
                     /translation="SDALSGFGFVASASFTDSSIELQYQDDDIIKSLGNGPTPLPGLS
                     KQVYNFTAYYERNGFEFRVNNRRRSDFIGEIGNFNGARTLRYVVGENITDAQIGYAFG
                     EGSFKGLGVQLQVSNLTNESYRTYAGTKDRPLENIEWGRTVLLGVNYKF"
BASE COUNT         2436 a         5791 c         5287 g         2355 t
ORIGIN      
        1 gcgccagcgc ggcgatcagg ttggtctcga tgatcgcctt gttgcgctcg aagccgccct
       61 tgccgaccgg catgccctgg ccgatgccgg cgttgaccac cacccggtcg agctcgccga
      121 aggcagcgcg gaacccgcgc acgacctcga atacccgcgc gtggtcggtg acgtcgagga
      181 cctcgaccag cacccgcacc cccgggtgcg cggcttcaag ctcctccttg agcgcctgca
      241 gccggtccaa ccggcgcgcg cacagcgcca ggtcatggcc gcggcgggcg aattcgcggg
      301 ccatccccgc gccaaggccc gaactggcgc cgatgatgac cacgttcgac ttgcgcatgc
      361 ctgccttcat ccccaatgcc ccgatccgcg atggcgccag cggcgctaac gatacgtcac
      421 cagtcccgca tgcctgcctt cgaagtgcgc atggccgttg agggtggaca ggctcacgcc
      481 ctcgcgcccc cgcaccagct tggtcacccc gcaattcacc agcgcccagc tcaggcggaa
      541 gccatcgctg tccgggatcc gcagcaggtg ctgggccacc gccgcgatcg gtccgccgga
      601 ggtgaacacc agcgcatcgg acccgcccgg caacgcatcg agcaccgacc ccagcgcctg
      661 catgcaccgc gcacggaaag cggcccagct ctcgctgtag tcggcgtcgt gcgcgccacc
      721 cacccaacgc gccaccgcct gttcgaacat ggcctggaac gcccgttgcg gatccggttg
      781 cgccatcatc tcggcgcgtg cgcgctcggg gtcggcgaaa tcgggccgca aggcggtgat
      841 cacccgcaca tggtcgaatt cgttccagcc ggcatcgctg cgccaggcgg gggcgaggcc
      901 catggcctcg aggcaggcct gcgcggtctg ccgatgccgc tgcaggtcgc cgctggccac
      961 caccgcgaag cccgcctgcg cgcgcgcaag cgcttgcccg agcacctgcg cctgctgctg
     1021 gccgcgttcg ctgagcacgt cgtagtccgc ggcaccgaac gaggcctgcc cgtggcgcac
     1081 caggtagacc gcgctcatgc cgccacccgc tggcgtgcga tcgcgcggcg gcagcgccag
     1141 tgcaggtagt gcacgaacag ccagaaccgc ttgaacgcgg ggttgcgggt ctgcccgtgg
     1201 tggtagcgga agtagatctg ctgggcgatc gccgacaacc ggaacagccc gtatacctcg
     1261 tggaaggtca cgtcgccggg atcgaacccc atcctgccgc agtagtggtc gatcacctcg
     1321 cggcgggtca gcatgcccgg caggtgggtg ggctggcggc gcatgccttg cgccatgaca
     1381 tcgtcgccag cctccaccca gtaagccagg gtattgccga ggtccatcag cggatcgccg
     1441 agggtggcca gctcccagtc gagcaccccg accacgcggg tgggatcgtc cggatccagc
     1501 accgcgttgt cgaagcggaa gtcgttgtgg gtgacgcaga tgcgctcctg cgcgggcagg
     1561 ttcgcggcca gccacgccat gatccgcttg ccgcgcggca cgttccaggt gcgcgcgtcg
     1621 cggtagcgcc tgctccagcc ctcgatctgc cgctgcgcgt aacccgcgcc cttgccgatg
     1681 ccctcgagcc cggccgcgcg atggtcgacc tggtgcagcg ccaccagggt gtccagcacg
     1741 ttcaggcaca gctgcttcac ctgcgctggc gagagctcga cgccgcgcgg caggtccttg
     1801 cgcggaatga ccccggccag ccgctgcatg acatagaaat cggtgccgat caccgcggga
     1861 tccgtgcaca gcgcgcgcat ctccggcacc agcgggaaca ccggcttcag caattgctgg
     1921 atgcggtact cgcggcccat gtcatgcgcg gatttcgcct tggtgccggc aggcgggcgc
     1981 cgcaggatca ggtcatcgtt ggcgtattgc agccggtagg tccagttcga cgcgccgccc
     2041 gggtattggg tcaccgtcgg ggtgccctgc agcgcgggca gcgcgccctt cagccatgcg
     2101 tccaccgcag caacgtcgag ctcctcgccg ctgcgcaccg cgcgcgcctc gtccgccggc
     2161 gccacgctca tcggctcgca gccttgcgcc gcatgcgcgc ggccacccgc gacagttgcc
     2221 ggcgcatcag cgccaggtac aggcgatacg gcagcaaccg cttcatccgc cagaaacgcc
     2281 tggcctccgg atgggtgagg atgtgcggat cgccgcgtgc catgccatcg aagatgcggt
     2341 cggcgatctc gtcggcaccg atccgcgcac gctccaccag gcgcttggtc atcccttcca
     2401 gcgcggggtt ggcggcgcgc atgttgccgg caagtccggt ccggaagaac gccgggcaca
     2461 ccacgctgac gcggatgccg tccgcttcca gctccagttg cagggtctcc gacagggcca
     2521 ccaccgccgc cttggtcgcg ttgtaggccg aggcctgcgg catgtgcacc agccccgcca
     2581 tcgaggcgat gttgaccagc tgcccgccgc ggccacctgc atccctcgcg cgcagcagcg
     2641 gcacgaacac cttgcagcca cgcaccaccc cgagcaggtt gatgtccacc gcccagcgcc
     2701 agtcttcgag cgaggtttcg gcgatgccgc ccatctgcgc gacgccggcg ttgttgacca
     2761 ccacgtccac cccgccccat tcgcgctgca accagtcggc ggccgcctgc agcgcgtcct
     2821 cgcgggtgac gtcgcaggcc agcgcgtggc tgcttggcga atgcacgcgg atcgcggcca
     2881 gcgccccggc gcagcgttcc atgtcgaggt cgccgatgca cacccgccag ccggcgcgcg
     2941 catggcgctc ggcgagcgcg cgaccaagcc cggacgcgcc gccggtgatg aagatgcgct
     3001 tgccgttgtc tgcaatgctt gcctggtcca tggccgggtc agccacgcgg cttgtatttc
     3061 gcgagttcca tccgcgccac catcgcccgg tgcacctcgt ccgggccatc ggcgaagcgc
     3121 agcgcccgcg cgatcgcgaa cagcccggcc aacggcaggt cgttgctgac gccggcgccg
     3181 ccatggacct ggatcgcctg gtcgaccacg gcctgcagca tgcccggcgc caccaccttg
     3241 atcgcggaga tctcggtcat cgcctgcttc acgccgaccg catcgatctt ccacgcggca
     3301 tacagggtga gcaggcgcgc ctggtcgatc gcgatccgcg catcggcgac gcgctccagg
     3361 ttgccgccca ggcgcaggat cggcttgccg aacgcgaccc gctccagccc gcgccggatg
     3421 gtgagttcca gcgcgtgctc ggccgcgccg atcgcgcgca tgcagtggtg gatgcggccc
     3481 ggcccgagcc gcccctgcgc gatcgcgaag cccttgccgc ggccaagcag caggttgtcc
     3541 ctgggcacgc ggacgccgtc gaacagcacc tcgccatgcc cgtgcggcgc atcgtattcg
     3601 ccgtgcacgg gcaacatgcg ctggatgcgc acgccgggcg tgtccatcgg caccagcacc
     3661 atcgcgtggc gcgcatgcgg ctccgcgtcg gggtccgata cccccatgaa gatggccagc
     3721 cggcagcgcg gatggccgat cccggtcgac caccacttgc ggccatcgag caccagttcg
     3781 tcgccgtcct cgcggatcgt cgcctgcatg ttggtggcgt cggacgaggc cacgtccggc
     3841 tcggtcatgc agaacaccga gcggatctcg ccggccagca acggggtgag ccagcgcgcc
     3901 ttctgcgcct cgctgccgta gtggtacagc acttccatgt tgccggtgtc cggcgcgctg
     3961 cagttgaaga tttcggcgag gaactcgttg cggcccatca cttcggccag cggcgcgtac
     4021 tccagcgtgc tcagccccgc gcccagcgcg gcatccggca ggaacaggtt ccacaacccg
     4081 gcggcgcgcg cgcgggactt ccattcgtcc atcggcgccg gcacctgcca ggcgttccag
     4141 tcggcgccgg cgttggcggc gttcgcgcgg cggtattcct gctcgcgcgg ggccacgtgt
     4201 tcggcgatga acgcctgcat gcgctgcagg tagtcccgcg tcttcgcgct cggggcgaag
     4261 tccatcagcc gaccttcagc agcagcttgc cggtgttctc gccgcggaac aggcgcagca
     4321 gggtgtccgg gaaggtatcg aagccctcca cgatgtcctc gcgcgacttc aggcggccgt
     4381 ccgccagcca gcccgccatg tcctgcgcgg cctcgccgta gcgcgatgcg tagtcgaaca
     4441 ccaccatccc ctgcatgcgc gcgcggttga ccagcagcga caggtagttg gccgggccct
     4501 tcacgccttc ggtcgtgttg tattgcgaga tcgccccgca gatcaccacc cgtgcatgca
     4561 tggcgagctg gcccagcgcg atgtcgagga tctcgccgcc gacgttgtcg aaatagacgt
     4621 cgatgccctt cgggcaatgc tggcgcaatg ccgcgcgcac gtcctcgcgc ttgtagtcga
     4681 tcgccgcatc gaagccgagt tcgtcgacca ggtagcggca cttgtccgcc ccgccggcga
     4741 tgccgaccac ccggcagccc ttgatccggg cgacctgccc gaccaccatg cccaccgcac
     4801 cggccgcgcc ggacaccacc acggtgtcgc cctgcttcgc ggcgccgacc tccagcaggc
     4861 cgaagtacgc ggtcatgccg gtcatcccca gcacgctgag gtaggtcgga agtggtgcca
     4921 gcgacgtgtc gaccaggcgc gcgcccttgc cgtcggaaac cacgtactgc tgcacgccga
     4981 acacgccggc gacgcggctg ccgaccggga atctcggatt gttggaggcg atgacctcgc
     5041 ccgccgaata cgcgcgcatc acctcgccga tggcgaccgg cggcacgtaa gacttgcctt
     5101 cgttcatcca gccgcgcatc gccggatcca gcgacacgta ctcgatgcgg accaggaatt
     5161 cgccttcgcc gggttcgcgc acgggctcct cgacgaggct ccagtcgctg gcccttggca
     5221 ggccgaccgg acgtgcggcg aggcggaact gacggttgct ggttgcgttc atgcgatccc
     5281 gttgctggat ggtttcccgc atcctagggg ccgcttgcca ttgaatacag tgatattttg
     5341 tactcaccag caatgaacat cattcattca aatgcggggc aactcgatct caacctgctc
     5401 aaggtgttcg aggcggccta tcgcgagcag cacctgggcc gcgccgcgca gagcctgcac
     5461 ctgaccccgt ccgcggtcag ccacgcgctg cgccgcctgc gcgcccagct cggcgacccg
     5521 ctgttcgtcc gcgacggccg ccgcatgctg cccaccccgg cctgccagcg gctggccccg
     5581 ccgttgctgg agacgctggc gcaactgcgg cagtggctgc agcagttgca gcatttcgat
     5641 ccggcgcagg cccggcaggc gttccatgtc ggcatgccgt atgccatcga ggcgatgctg
     5701 ctgccggcga tctaccgcct gctgcgcgaa cgcgcgccac gggcgaccct ggccagcgtg
     5761 ggttacgaac ggcgcgacct tgcgcgcgcg ctcgccgccg ggcaactgga cctcgcgatc
     5821 gacgttgccc tgcccgccag cgaaccggtg cggcatgcgg tgctgctgca gcagggcttc
     5881 tgcatcgcgg tgcggtcgcg gcatgcactg aagcggcggc cgaccctgcc gcaatacctg
     5941 caggccccgc acgtggccgt ctccagccgc gccagcggcg cggtggtgga agacatcgcc
     6001 ctgctcaacc tcggcctgca acggcggatc gcgttgcgtt gccaggatta ccaggcggcg
     6061 tgccgggtgg tcgccggatc cgaccacctg ctgaccttgc ccaccgggct ggcgcggcag
     6121 atggccgggc gttcgctgcg cctgctgccg ctgcctttcg agctaccgcc gatccagccg
     6181 catctttact ggcatgccaa cctggccgtg gacccggcca accagtggct gcggcaactt
     6241 gttgtcgatg cctgcaggac gctcgacggc ggcaccggtt aggtgcggcc gaacaccagc
     6301 gtggcgttgg tgccgccgaa gccgaagctg ttcgacatca ccgtgagcag gtcggcggcg
     6361 cggctctcgc gcacgatcgg gaaaccctcg caggccgggt ccagcctggc gatgttggcg
     6421 gagccggcga tgaagcggtc gcgcagcatg agcaggctgt agatcgcctc gtgcacgctg
     6481 gcggcgccca gcgaatgccc gctcagcgcc ttggtcgacg acagcggcgg ctcgttgccg
     6541 gcgccgaacg cagcgcgcac cgcctgcaac tcggtgacgt cgccgagcgg ggtggaggtg
     6601 ccgtgggtat tgaggtaatc gatcgggcga tccagccctt ccatcgccat ctgcatgcag
     6661 cgcaccgcgc cctcgccgct cggcgccacc atgtcggcgc cgtcggaggt caccccgtag
     6721 cccagcaatt cgccgtggat gcgggcgccg cgcgcgactg catgctcgta ttcctccagc
     6781 accagcatgc cgccgccggc cgcgatcacg aagccgtcgc gatccgcgtc atagggccgc
     6841 gaggcgattt ccggggtgtc gttgaagtgg ctggacaacg cgcccatcgc atcgaactgg
     6901 caggtcatgc tccagtgcgc ttcctcgccg ccaccggcga acatgatgtc ctgcatgcca
     6961 tgccggatca tgtcggcggc ggcgccgatg cagtgcgcgg aggtcgcgca ggcggccgac
     7021 agcgagtagc tgacgccctt gatcgcgaat accgtggcca gcgccgccga caccgtcgaa
     7081 cacatcgtgc gcggcaccat gtacgggccg accttgcgca cgcccttctg ccgcagcagg
     7141 tcggcggtct cgacctgcca ctgcggcgag ccgccgccgg aaccggcgat caccccggtg
     7201 cgcggctggc tgacctgcga cggcagcagc ccggcgtccg cgatcgcgtc gcgcatcgcc
     7261 accattgcat acgccgcggc atcgcccatg aagcgcttct gcttgcggtc gatgctggca
     7321 tcgaggtcga tctccggcac ccccgccacc tgcgaacgca ggcccagccc ggcgtattcc
     7381 gggatctggc gaatgccgct gcgacccgcg cgcagcgccg ccgacacgct ctccaggcca
     7441 ttgcccaggc tggacacgat gcccatgccc gtcaccacca cgcgccgcaa catgctcact
     7501 tcgcctccgt tgactggaac agacccaccc gcatgtcggc ggcggtatag atttcacggc
     7561 catcgacata ggtgcggccg tcggcgatca ccagcttcag gcggccgcgc aggacgcggc
     7621 ggatgtcgat ctcgtagctg accaccttcg cgtccggcag cacctggtcg gtgaacttga
     7681 tctcgccggc gcccagcgcg cggccgcggc ccggttcgcc cagccatggc aggaagaagc
     7741 cggtgagctg ccacatcgca tccaccccca ggcaacctgg catcaccggg tcgcccagga
     7801 aatggcagcc gaagaaccac aggtccgggt tgatgtcgag ttcggcgcgg atgaagccct
     7861 tgccgtgcgc gccgccgtcc tcggtgatcg aagtgatgcg gtcgaacatc agcattggcg
     7921 gcgcgggcag gcgggcattg cccgggccga acatctcgcc gcgcgcgcag gccagcagtt
     7981 gttcgcgatc cagcgaggac ggacgggtca tggagcagcc agcgggggaa aacccgattc
     8041 tacggccgcc gcacccgaca tgcctatgcg tatgcgcggc gtgcgggcgc cgtgctcgcc
     8101 gcggcggtga aaacgaatgc accggcgccg caccgcccgc cgtattaaag tccggacatc
     8161 cccacgtccg gaccacccgc gtgacgcgac ccgcgcccgc ttccctgttg accatcttcg
     8221 gcgccaccgg cgacctcgcg cagcgcatgc tgctgccctc gctgtacagc ctgcaggcgg
     8281 agaacctgtt gccgcccggc ctgcgcatcc tcggcagcgc gcgcagcgac ctcgaccgcg
     8341 aggccttcgc caacctggtc gccgatgcga tcaacgaacg catcccggcc gtcgaacgcg
     8401 acgacaccgc ccttcgcggc ctgctggaac ggctggacta cgccgccgcc agcgtcgacg
     8461 acgcggcctc catgcaggcg ctcgcggcca gggtgtccga cctgcgcaac ggcgacgtgc
     8521 tctaccacct gagcaccgcg ccgcgcttct acgtgccggc gtgccacgca ctcggtgcac
     8581 acggcgccgc cgacgcacgc acccgggtga tgctggaaaa gccgatcggc aaggacctgg
     8641 ccagcgcggc ggcgatcaac gacggcgtgg ccgaggtgtt cgacgaggac cgcgtctatc
     8701 gcgtggacca ctacctcggc aaggaaggcg tgcagaacct gatcgcgctg cgcttcggca
     8761 atgcgctgtt cgagccgttg tggaacgcgc gccacatcga gcaggtgcag atcaccgccg
     8821 gcgagacggt cggcgtcgag ggtcgcggcg actattacga cgattccggg gcgatgcgcg
     8881 acatgctgca gaaccacctg ctgcagttgc tgtgcctggt ggcgatggaa ccgccggcgc
     8941 gcttcgagcc gaccgcggtg cgcaacgaga agatcaaggt gctgcgctcg ctgcgcgcga
     9001 tcgggcacgg gcaggtcgcc accgaatcgg tcgccggcca gtacaccgcg ggcgcgatcg
     9061 acggcgtcgc ggtgcccggc tacgtgcagg aactcgggcg cgacagcggc accgagacct
     9121 tcgtcgccct gcgcgcgcac gtggacaact ggcgctggtc cggggtgccg ttctacctgc
     9181 gtaccggcaa gcgcctgccg tcgcgctgca ccgagatcta catccagttc cgcaaggtgc
     9241 cgtattcgat cttcggcgga cacgcggccg cggacatgcg gtcgaacggc ctgctgatcc
     9301 gcctgcagcc ggaagagcgg atcgaactgg acctgatggc caagaccccg gggctggacc
     9361 gcagcggcct gcgcctgtcg caggtcgcgc tggacctcga tttccacgag gagttcgcca
     9421 accagcgtcg ccgcatatcc tacgagcgcc tgtacctcga cgcgatcgag ggcaatggca
     9481 ccctgttcgt gcgccgcgac gaaaccgaaa ccgcctggca ttgggtggat gcgatcctgg
     9541 atggctggcg cacggccggg gtggcgccga agccgtaccc cgcgggcacc tgggggccgg
     9601 gcgcggcggt atcgctgatc gagcgccacg gccacggctg gcgcgagtga tgggctggat
     9661 cgaacacgac catcccagta ccgacgcgct ggtcgaacag gtcgcgcagc aactgcaaca
     9721 tgcctgccgc gaggcgatcc acgcacgcgg cgccgcctgg ctggcgctcg ccggcggacg
     9781 cacgccattg ccggcctatg cacggctggc cgcatcggag ttcgaaggca gcatcaccgc
     9841 ggtcccgacc gacgagcgct gcgtcccgca cgaccatccg gcctgcaacc tgcgcgcact
     9901 gcgcgaggcg ttcgcggcga atcgcggcat caccaccgcc ggcatcaccc gcgaggacgg
     9961 cgatgaaggc gcatcgctgg cgcaggcgcg cgcgctgctt gcgctgaatg cgcagccatt
    10021 cgacctcgtg ctgctgggga tgggcacgga cggccacttc gcctcgctgt tccccggcgc
    10081 ggccaacctc gccgaggggc tggcgatgga aagcgggatc gacgccatcg ccaccctgcc
    10141 cgagccgttg ccggcggaag cgccgttcgc gcggatcagc ctgaccctgc cgcgcctgct
    10201 gcgcgcgcgg acgatcctgc tggtcgccac cggcgaggac aagcgccggg tgctgcgcca
    10261 ggcccggcag caggaccgcg gcggtcatcc ggttgccgcg ctgctgcatg cgcgcgacca
    10321 tcgcgtacag gtccactgga gtccctgatg ccgcacttgc accccgtggt cgaacgcgtc
    10381 accgcccgca tcgcggagcg cagcaggcgc ctgcgcggcg actacctggc gcggatggac
    10441 gccgcccgcc tggatggccc ggcgcgccgc gagctcggct gcggcaacat cgcccacgcc
    10501 ttcgccgccg ccggcgcgga caaggcccag ctgcgcaacg gcagtggcgg caacctcggc
    10561 atcgtcaccg cctacaacga catgctcagc gcgcaccagc cgatggagca gtaccccacg
    10621 ctgatccgca tggccgcgcg caacgccggc ggcagcgcgc aggtcgccgg cggcgtgccg
    10681 gcgatgtgcg acggcgtcac ccagggccgc gccggcatgg aactgtcgct gttctcgcgc
    10741 gatgcgatcg cgatggccac cgcgatctcg ctctcgcacg cgatgttcga cggcgccctg
    10801 atgctcggcg tgtgcgacaa gatcgtgccc ggcctgctga tcggcgccct gggcttcggc
    10861 cacctgccag ccatcttcgt gccggcgggt ccgatgccat ccggcatccc caaccggcag
    10921 aaggccgaag tccgccagcg ttacgcgctg ggcaaggcca gccgcgagga actgctggac
    10981 gccgaggccg cgtcctacca cgcgcccggt acctgcacct tctacggcac cgccaactcc
    11041 aaccagatgc tgatggaagt gatgggcctg cacatgccgg gcagcgcgtt cgtcgccccc
    11101 gggacgccac tgcgggatgc cctgaccgtg gccgccaccg agcaggcact gcgcatctcc
    11161 gcgctgggca gcgattaccg cccgctggcg cgcaccgtgg acgagaaagc catcgtcaac
    11221 gcgatggtcg gcctggccgc caccggcggt tccaccaacc acgcgctgca cctggtcgcg
    11281 atcgcccgcg ccgccggcct cgtgatcgac tgggacgacc tcgacgaact gtcgaaggcg
    11341 accccgctgc tggcccgcgt gtatccgaac ggcgccgccg acgtgaacca cttccacgcc
    11401 gccggcggcc tcgggttcgt catccgcgaa ctgatcgacg ccggcctgct gcatccggac
    11461 atcgcctgcg tgcatggcgg cgacctgcgc cagcaggcgc tggaaccgtt cctcgacggc
    11521 atcaccctgc gctggcgcga gccaccggcg cgttcgctcg acgccagcgt gctgcgcggc
    11581 gtggccgcgc cgttcgacag cgagggcggc ctgcgccggc tgcagggcaa cctcggccgt
    11641 gcgacggtga agatttccgc ggtcgcgccg gaattccgcc ggatcgaggc acccgcgcgc
    11701 atcttcgaat cgcaggacgc gctgctggac gccttcaagg ccgaccagct cgatggcgat
    11761 ttcgtcgcgg tggtgcgcgg ccagggcccg cgcgccaacg gcatgccgga actgcacaag
    11821 ctgacgccga ccctggccgc cctgcaggac cgcggccagc gcgtcgccct gctcaccgac
    11881 ggccgcatgt cgggcgcgtc cggcaaggtc ctggcggcga tccacgtcac cccggaagcc
    11941 gcctgcagcg gtccgctggc caagctgcgc gacggcgatc cggtccgcat cgacgccgaa
    12001 gccggcacca tggaggcgct ggtggaggcc ggcgcatggg ctgcgcgcgc atgcgcgaag
    12061 cctgccctcg ccggcaacca ccttggccat ggccgcgagc tgttcgggtt gatgcgccgg
    12121 caggtcggcg ccgccgaaca gggcgcgtgt tcgttgttcg tggacgagga cggcgagtga
    12181 ccacgctggc gctggtcggc gacatcggcg gcaccaatgc gcgcttcgcg ctgaccgacc
    12241 tggccgcgcc cgcgatcgaa ctgcagcagg cgcaatcgct gcgcaacgcg gatttcgcca
    12301 gcgtccagca cgcggtcgag cattacctgc aggcgatcga cgcgcggcct aagcatgccg
    12361 cgctggcggt ggcctgcccg gtcggcagcg acgagatccg cctgaccaac cgcgcgtggt
    12421 cgttcagccg gcgcgaactc cagcgcacgc tgcagctgga gcagctgcgc atgatcaacg
    12481 acttcggcgc ggtggcatgg gcggtgccct cgctcgaacg cgacaacctg gtcaccctgc
    12541 atggcgatcc cgcggcgccg ctgcgcggcc cggtcagcgt gctcggcccc ggcaccgggc
    12601 ttggcgtcgc cctgctggtc ggctcgcgcg agcacggctg gcacgcggtg gaaaccgagg
    12661 gcgggcatac cagcttcgcc ccgatcggcg acgaggaacg cgcgatctcc gcatggctga
    12721 ccgcgcagca cggacgcacc tcgaccgaac gcctgctgtg cggcaagggc ctgtcggaga
    12781 tcgatgcagt cctgcgcggc gccagtgcgg tgctgccatc cggactgccg gtcccgggcg
    12841 acacctcgtt gctcaagccg gcgctgcgcg accccgcgga gatcgtggcg gcggcgctgg
    12901 agggccacga cctggccgct cggcagaccc tggcgcgctt ctgcgcggtg ctcggcagcg
    12961 tcgccggcga ttgcgctttg atccagggcg cgcgtaccgt ggtcatcgcc ggcggcatcg
    13021 tgccgcgctt catcccgttc ctgcgcagca gcgccttccg cgaacgcttc ctggccaagg
    13081 ggcggatggc aaccctgctg gaatcggtgc cgatccacgt catcacccac ccgcaaccgg
    13141 ggctgctcgg cgccgccacc gcattgcgct catcggagct ttgaacatcg catgaccgac
    13201 accgccagcc acgccagcca ggtcgacgcc gtgttcgcgc gcgcgccggt gatcccggtg
    13261 ctcaccatcg agcgcatcga agacgccatc ccgctggcgc gcgccctctc ggacaacggc
    13321 ctgccggtgc tggaagtcac cctgcgcagc gcctgcgcgg tcgacgccat cgccctgatc
    13381 gcgcgcgaac tggcggatgc ctgcgtcggc gccggcaccg tgttgaatgc gcgcgacctc
    13441 gacgcggtgg cgcaagccgg cgcacgtttc gcgatttccc ccggcgccac cgacagcctg
    13501 tatcgcgccg cggccgaatg cccggtcccg ctgatccccg gcatcgccac cgccagcgaa
    13561 ctgatgcgcg ggctcgaaca cggctggcaa cgcttcaagt tcttccccgc cgaatccagt
    13621 ggcggcatcg gcgcgctcaa gggcttcgcc ggcccgttcg cgcaggtgcg gttctgcccg
    13681 accggcggga tcgacgcggc gaaggccccg gcctacctcg gcctgcccaa tgtcgcctgc
    13741 gtgggcgggt cgtggatgct gcccgcggac gcactggcgg cacgcgactg ggcgcgcatc
    13801 ggcggccttg cgcgcgcggc ggccgcgctc acgcgctgac cgcaggcgtg gggcttgccc
    13861 cacgcgcgcg gcagcggggc aagccccgct ctacacggcg ggggacggtg gttccggcgc
    13921 gcccatcacc cggcccagca cgactgcgac caggatggtc gcggccaggg tgatgaacat
    13981 caggtgcagg tagtgcagcg gcgtccacgc gaaggtgaac atcgcgtaca gcgccacccc
    14041 gaacaccagc gcgatgcgga tcgcggtcgc gttcgcgcca cgcacgaaga tcgcgacgat
    14101 gaaggacgcc agcaccggca tcgagtacag gccgttcaac tgctgcagcg tgccgatgat
    14161 gctggtggcg ttctggtaca gcggcaccat cgccagcgac accagcatga acaccagttg
    14221 cacgacctgg ccgatgcgct tgccgtccgc cttcgggttg accatcggca ggtgcaggtc
    14281 gaccgtgtac agcgccgccg ccgagttgag tccggcgttg tagctggaca gcaccgcgcc
    14341 ggtgatcgcc gccgcgaatg cgccggacag ccacggcggc aacacgtcgc cgaccagccg
    14401 gccgtaggcg ttgtcgtcga tgtcgccgta cagcttgaac gcgatgatgc cgggcaggat
    14461 cacgatgaac ggggtcagga acttcaacgc cgccgccgca tagatgccct tctgcgcctc
    14521 gcggatgtcc ttcgccgcca gcgcacgctg cgcgatcagc atgttggtgc cccagtagaa
    14581 gatgtgggcg aacaccatcc cggtcatcaa cgtcggccac gggatgtccg aatccggacc
    14641 gccccacacc gtcagtcgct ccgccgggat gccggagaaa tcccagccga tcctgtccag
    14701 cgcgaaccag gtgaccagcc cgcccatcac cagcaggatc acgcccatgt aggtgtcgga
    14761 gatcgcgatc gcgcgcaggc cgcccagcgc agcgtaggcc aggccggcca gcgcgaacag
    14821 gatcgagatg gtcaggatcg acatgtccgg ctcgaacatc gacttcatga agcgcgaacc
    14881 ggtgtacagc accatcggca acaggatgaa catgtagccg agcaggaaca gcaccgacac
    14941 cgtgcgccgc agcgatgccc cgttcggcaa ccgcttctcc agcagttcgg tggtcgtggt
    15001 gcagtcgtac ttgtagtaca tcggcaccag caccttggcc agcaccagca ggccggccgc
    15061 ggcgccgaac tcccaccaca cctgcagcat gttctgcgcg ccattcatgc cgacgatctg
    15121 ctcggcgctg atgttggtca gcagcaggct gccggcgatg aacggccagc tcaggtgctt
    15181 gccggccagg aagaaatcct gtgcgctgtc gccggccttg gcctccttgc cataacgcca
    15241 ccacgtcgcc agcgcgacca gcgcggtcaa ccccaggaat accgccagtt gcaccatgtt
    15301 ggtgtccatc gattccaacc ctccgcggcc tgccggcgca cgctgcgcaa aaaagacccc
    15361 tgcctgtacg ggacaggcag gggtttggtc acatcattgc atcgggcgcg gaatcagaac
    15421 ttgtagttca cgcccagcag cacggtacgg ccccactcga tgttctccag cggacgatcc
    15481 ttggtgccgg cataggtgcg gtaggactcg ttggtgaggt tgctgacctg caactgcacg
    15541 cccagcccct tgaacgaacc ctcgccgaac gcgtagccga tctgcgcatc ggtgatgttc
    15601 tcgccgacca catagcgcag ggtgcgcgca ccgttgaagt tgccgatctc gccgatgaag
    15661 tcggagcggc ggcggttgtt gacgcggaac tcgaagccat tgcgctcgta gtaggcggtg
    15721 aagttgtaaa cctgcttgga cagacccggc agaggggtcg gcccgttacc caagctcttg
    15781 atgatgtcgt catcctgata ctgaagctcg atgctgctgt cggtgaagct ggcgctggcg
    15841 acgaagccga acccgctcaa cgcgtcgct
//
LOCUS       DAORUK010000034        15860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_3435_length_15860_cov_18.826574, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000034 DAORUK010000000
VERSION     DAORUK010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15860
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3435_length_15860_cov_18.826574"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="PK818_03745"
     CDS             <1..137
                     /locus_tag="PK818_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HQY81881.1"
                     /translation="MKKRWQETQLCLDLFAQQADKSLTIRAKQLYHDREEITVFATSR
                     "
     gene            complement(148..1350)
                     /locus_tag="PK818_03750"
     CDS             complement(148..1350)
                     /locus_tag="PK818_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY81882.1"
                     /translation="MNRRHRLDAVVAGGGVVGACTALMLAREGLQVALVEPKRPPAWQ
                     RGSRDLRVYAFAPDNACLLDDLGVWQPVLAARAQPYRGMRVWDAAGGDALAFDADALG
                     QRQLGWIVENTLLVDALWNALPAAGVRLHCPARIEAVEQSEAGVRLQLDDGVALDAKV
                     AIAADGANSALRGMAGIGTRKRDYAQKGLVAFVASGVPHRDTCWQRFLPTGPVAFLPF
                     ADDGDASVQGRLGSIVWTLPDAEAERLGGIDEAGFARELGDAFGGELGTMRLLSARAA
                     FPLRRQLAETYFSGRVLLVGDAAHAVHPLAGQGVNLGLRDVAALRRLVGDAAARGADF
                     ASPQRLARWARTRKSENAVNALAFEAINHAYSNDAPWPTLLRGHALGFAGKLPPLSRA
                     LWRHAAGV"
     gene            complement(1347..2585)
                     /gene="ubiH"
                     /locus_tag="PK818_03755"
                     /gene_synonym="visB"
     CDS             complement(1347..2585)
                     /gene="ubiH"
                     /locus_tag="PK818_03755"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HQY81883.1"
                     /translation="MTATPNDTMPLHDVLIVGGGLVGASLAIALDAQGLDVGMLEATP
                     AGALPAVFDQRNLSFAEATVNALAALGVMPLLAAPTGAIRRIHVSRAGDFGRIRLDAR
                     AHGRDRFGQVVVARDFGEALETRLSQLSRLTRYRPARFVGLADADADPAQRVVRIVDA
                     GGERELRTRLLVAADGSRSSVRDALGISAREHDYAQTLLVARLRTQRPPDGTAWERLT
                     DSGPTALLPRGDGHYGLVHGVARDHADAVAALDDATFLARVQDAFGWRAGRFLACGPR
                     SAYPIVGCVAAQLVAPRAVLVGNAAQSLHPLGAQGFNLGLRDALTLAECIADGGDPGA
                     PALLARHAQRRAQDRERTLAFSDGLARLTSNAAPLLRPLRSAGLLAGDAMGWLQGWLV
                     GGAMGYRGDVPALCRQGGAA"
     gene            2628..3212
                     /locus_tag="PK818_03760"
     CDS             2628..3212
                     /locus_tag="PK818_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81884.1"
                     /translation="MNRPLLSSGPDLPAAGPLALAALIFIAALSRVLPHPPNFSPIEA
                     VALFGGAYFASRRWALLVPLVAMFASDLVLGLANGGIYWDYFRSAGYLLVYACIALST
                     VLGFGLRGRVSGSRVLGYSLAGSMLFFVVTNFGAWLSDPAYPKTIAGLVAAYLAGVPF
                     FQWTVLGTLAYAALLFGGFELLRKHVPALRAQTA"
     gene            3222..3773
                     /locus_tag="PK818_03765"
     CDS             3222..3773
                     /locus_tag="PK818_03765"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014502064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HQY81885.1"
                     /translation="MGNRLSKIYTRTGDDGTTGLGDGTRVAKDSARVNAYGTVDEANS
                     CIGVLLAGEVPDDVRALLTRIQHQLFDLGGELCIPGHAAIFDADIDALEARLDHYNEG
                     LPVLKEFILPGGGEAAARCHVARTVVRRAERDTVTLSRHDAVRPQAIRYLNRLSDLLF
                     VLSRVLARAGGHGEVLWEHDRRR"
     gene            3787..4698
                     /locus_tag="PK818_03770"
     CDS             3787..4698
                     /locus_tag="PK818_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004144562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HQY81886.1"
                     /translation="MRIYTHPACLWHDTGPEHAERPERLRSVIDALIAAFPELDWREA
                     PRASRGQLLRAHAPDLLQAVLETNPRERMLLDPDTVLSPASAEAALRAAGAGVAAVDA
                     VLGDTTTRAFCAVRPPGHHATHDVAMGFCLFNSIAVAALHALDAHGLERVAVVDFDVH
                     HGNGTQAIFERDPRVLYASSHQWPLYPDSGARSETGVGNIFNAPLPPETDGARFRAAW
                     SGSLLPAIDAFRPQLLLVSAGFDAHRRDPLAELRLEAGDFGWIARELVQLANRHCRGR
                     IVSMLEGGYDLEALAESSVAHVRELLA"
     gene            5003..7300
                     /gene="lptD"
                     /locus_tag="PK818_03775"
     CDS             5003..7300
                     /gene="lptD"
                     /locus_tag="PK818_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HQY81887.1"
                     /translation="MRPALRLLPLSLCIAVSLNARAGDDMPQNWSLCPVQDVVKPFAE
                     AQAAPEGIGIDNTEQATDIEGDALTGTEENPVFNGNVTLRRGDQFMGADQLTYDKGQE
                     HYTAEGSIRYQGSGLRMTAARAEGDQAKDVHTIHDLDYQLLANRGNGGAERVTLTGDI
                     GSLHGATYSTCAPDARHWELRANRIDIDTEKGMAVARGATLRVGQVPIIYLPWFMFPT
                     DERRRTGLLFPSVSNSSRNGLDWKQPIYLNLAPNYDATLNPRLMTNRGVQLGAQFRYL
                     VEGGAGVVDGTWMPDDDLRDSDRSMIRYGAFQNVNRNWQARANLNWISDSRYYEDFSS
                     SLDGLSQSTSFSEIGVYGRGHGWEAGITSDYWQLADFTLTEADLPYNRVPRAYVHWER
                     GLGLHFVGGVDAEMVRFRHPLFDAGSRLDVKPWISLPLEGDSWFVKPTAAFRHTSYEL
                     DESLTASRSRSRSQPIFSLDSGLFFDRDTRVKGKDYLQTIEPRLYYLHVPYAYQDDMP
                     LFDTQPLTFSWGQLFRDNRYSGADRQADANQLTLALSTRMIRQSDGRERFSASLGQIR
                     YFDESRVSIPGEPVTEKGRSAWVADANYAPTDRWTFGASYQWDPKFNRTDLASIRARY
                     LLPDDGVFNLGYRYRRSLLEQADVSFLYPINPTWSVVGRYYYSMYDSKALETIAGLQW
                     DSCCVAVRLVARRYVHNRAGDLSNSVLLEIELKGLGSAGQDTRRALRRAILGYNRDDL
                     YLVPPQTATGQPTPPAIDTVNDASP"
     gene            7297..8643
                     /locus_tag="PK818_03780"
     CDS             7297..8643
                     /locus_tag="PK818_03780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY81888.1"
                     /translation="MTLAPTRSLLAIALAGLLFAAGAHAQSYAGTAPVDRIAAVVNED
                     VILRSELDRAVANIRAQYAGKEAQLPPQDVLERQVLERLVLMRLQLARATDSGITASD
                     EDLERAVQGVAEQNGMSVDQLRARISQDGMSFTEFRNNLRDEIVTQKLRQSFAQGRIN
                     VSEAEVDSAMTAAAASISQQFRLAHILVGTPDGASAEQLATAQKKIEGVKGLIDKGEM
                     SFAAAAVRYSDSPNALEGGDLGWRGLNEIPPAFAQTIQQMQPGQVIGPIRGASGFQLL
                     QLVETRSQAAGGGEQVTQFSARQILVKVDDTTDDAAAKAEAETLAARIAGGADFAKLA
                     SESSDDDATKRRGGDLGWFGADTYGNAFGMQVAALSDGQTSAPFKTDAGWVLVQRTSS
                     RQVMAGSDSLRAQVRETIGRRKLEDEWNRWTREMRGEAFVDVRGLDGAAANPATGG"
     gene            8658..9632
                     /gene="pdxA"
                     /locus_tag="PK818_03785"
     CDS             8658..9632
                     /gene="pdxA"
                     /locus_tag="PK818_03785"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HQY81889.1"
                     /translation="MRPRLALVPGEPAGIGPELVVRALRQEFDAELVVFGDAAALQRA
                     ARALSLPLELHRAGEAPASPRSTGIVDIPHPMAAAFGTPEPANAASVVQALLAAAQAC
                     VEGTCHGIVTGPVHKAAINDGGIAFTGTTGLLAAHAGREVVMMLANDIVRVALLTVHL
                     PLRAVADAVTPAALATTLRIVDAALRGDFGIAEPTIAVLGLNPHAGEAGHLGREELDI
                     IEPTLQRLRGEGLRLAGPLPADTAFLPGKLRGFDAVLAMYHDQGLPVLKYSDFAHAVN
                     ISLGLPYPRVAVDHGTALELAGTGMADPSSLLAAINACARFARNRMHA"
     gene            9629..10468
                     /gene="rsmA"
                     /locus_tag="PK818_03790"
     CDS             9629..10468
                     /gene="rsmA"
                     /locus_tag="PK818_03790"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HQY81890.1"
                     /translation="MSTRFTEPAKTSPSGFSAEAKKHLGQNFLHDTTVIAKIVQALDP
                     KPGDVIVEIGPGQGALTFPLLRRHGELTAIEFDRDLHAPLQAAAREHGTLHLVQGDVL
                     GVDFSALAAELACTGGQIRLVGNLPYNLSSPILFHALDHAAAVRDMHFMLQKEVVERM
                     AAGPGSKVYGRLSVMLQAYCTVTPLFIVPPGAFRPAPKVESAVVRLVPRPAAEVEVGD
                     RRDFANVVRAAFGQRRKTLRNALHGVADSALMEAAGVRPDARAEQVEVAAFVRLANLL
                     GGG"
     gene            10515..10895
                     /gene="apaG"
                     /locus_tag="PK818_03795"
     CDS             10515..10895
                     /gene="apaG"
                     /locus_tag="PK818_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HQY81891.1"
                     /translation="MGNTDYIIDIAVDTCFLDDQSAPEEGRFVFAYTIHIRNHGKVPA
                     RVLGRHWLITDSNGKVREVVGEGVVGEQPWLRPGEGFEYTSGAVLETDIGTMRGSYDV
                     LADDGTRFAAPIPAFTLSVPRTLH"
     gene            10912..11811
                     /locus_tag="PK818_03800"
     CDS             10912..11811
                     /locus_tag="PK818_03800"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HQY81892.1"
                     /translation="MSTWAIGDLQGCHDVTQRLLEKIRFDPAVDRLWFCGDLVNRGGQ
                     SIETLRLVHSLRAVSHVVLGNHDLSLLAIGERSPDEQRKVNPDLQGVLFAPDARELLD
                     WLRSQELAHVDRALGWMMVHAGLAPKWTTQLAEKHAREVEAKLHGDNYRKLLKNMYGD
                     GPEWSPRLNGAERDRAIINIFTRMRYCSPRGRIAFEHKGEPGSQPPGLYPWYSVPGHA
                     PRDLKVVCGHWSTLGLFVGHGVHAIDTGAVWSGKLTALQLDSEDLRVVQVPGRDVPAP
                     APRVRPPKPKQPSPQHRHDGQRR"
     gene            complement(11812..12330)
                     /locus_tag="PK818_03805"
     CDS             complement(11812..12330)
                     /locus_tag="PK818_03805"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HQY81893.1"
                     /translation="MRIALIAALDRNLAIGKGNALPWHLPDDLKRFKALTLGQPVLMG
                     RKTAQSLGRALPKRRNLVLTRGGHVPFDGMEAVASIADAKALAAADGAEALCVIGGGE
                     VYALALPLATHMHLTHVDAAVAGADAFFPRFDPGEWTVSVRQPHPADAAHAFAFAFVD
                     YTRNQPQACAAR"
     gene            complement(12338..13132)
                     /locus_tag="PK818_03810"
     CDS             complement(12338..13132)
                     /locus_tag="PK818_03810"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011260172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HQY81894.1"
                     /translation="MKQYLDLLGHVLEHGTEKPDRTGTGTRSVFGWQMRFDLAAGFPL
                     VTTKKLHLRSIIHELLWFLQGATNTAYLKDNKVSIWDEWADENGELGPVYGKQWRSWE
                     GADGQAIDQVRWVVDEIRRNPDSRRLIVSAWNVADLPKMALMPCHTLFQFYVANGKLS
                     CQLYQRSGDIFLGVPFNIASYALLTHMVAQVCGLGVGDFVHTLGDAHLYSNHFQQARE
                     QLARTPRALPTLKLNPGVKDIFGFRFEDVAIEGYDPLPAIKAAVAV"
     gene            complement(13155..14081)
                     /gene="lgt"
                     /locus_tag="PK818_03815"
     CDS             complement(13155..14081)
                     /gene="lgt"
                     /locus_tag="PK818_03815"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQY81895.1"
                     /translation="MPYLHQIDPIAIDFGTWNLPLLGSVHPQVHWYGVMYLLGFLVAW
                     WLGRRRIRAGRLPGVDDNGFGDLLFYAMLGVVLGGRIGYILFYGFPDFIANPLMILKV
                     WEGGMSFHGGLLGVLLAVGWWSRRQRLHFLDTVDFVAPLVPAGLGFGRIGNWIGGELW
                     GKPTQGTAFDGWGVVFPRSLPEPFASMDAATLKAQFDSGIVDSFARHPSQLYQATLEG
                     LAMFCVLVWYSRRPRPRHAVSGLFALLYGCFRFLVEFVREPDAQLGYLAFGWLTMGQV
                     LSTPLILLGLFWLWKSHSSPTLVPQPATEAAK"
     gene            complement(14134..14625)
                     /locus_tag="PK818_03820"
     CDS             complement(14134..14625)
                     /locus_tag="PK818_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQY81896.1"
                     /translation="MRRLFAHLFTGDAQGRFPEADMQRIAAAIADGEARHRGEVCFAV
                     EGALDWRDVLRGVQARTRALDAFAQLRVWDTAANNGVLVYLLLADHRIEIVADRGLRG
                     RVGDEQWRGVCLLMEERLRAGEYTDAVVDAIAAASALLAAHFPQAPGGMDEDELPNAP
                     VFL"
     gene            complement(14637..15557)
                     /locus_tag="PK818_03825"
     CDS             complement(14637..15557)
                     /locus_tag="PK818_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005910710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQY81897.1"
                     /translation="MHALRTCGWRLALASLLAWLPLACLAQQPAPVPPFDSPVVDTTG
                     TLDPATTRQLQQQALDLQQRKGSQLQVLVIPSTAPETIEQYAVRAFESFRLGRRGVDD
                     GLLLVVARDDRKVRIEVGYGLEGAIPDITAGRVIQEYLVPKFRQGDYAGGIVDATAQL
                     VKLVDGEPLPEPVASHPTGPDGGDNWMLALFAAFIVAQLVRGILARTPAGVRALFGGA
                     ASGGIAWLLSSAVLVGGIGVAIGFLVGLLPGAGNRRYARSHDWGGFGGGSGWGGGGSG
                     GGGWGGGSSGGGGWSGGGGTSGGGGASGGW"
     gene            complement(15598..>15860)
                     /locus_tag="PK818_03830"
     CDS             complement(15598..>15860)
                     /locus_tag="PK818_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010481850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQY81898.1"
                     /translation="QDYNTYIRSFPQNLVAMVFGYKQKPNFTVEDETRIADAPSVDFG
                     AAPAEPAPVPPVVEPAASVVNPPQPALPPVQGGNAPQPAPTE"
BASE COUNT         2462 a         5813 c         5376 g         2209 t
ORIGIN      
        1 cgatgaagaa gcgctggcag gaaacccagc tgtgcctgga cctgttcgcg cagcaggcgg
       61 acaagtcgct cacgatccgc gccaagcagc tctaccacga ccgcgaagag atcacggtgt
      121 tcgcgacctc gcgctgaggc ccgcgatcta gaccccggcg gcgtgcctcc acaacgcccg
      181 cgacagcggc ggcagctttc cggcgaagcc cagcgcatgc ccgcgcagca gcgttggcca
      241 tggcgcatcg ttgctgtagg cgtggttgat cgcctcgaac gcgagcgcgt tcaccgcgtt
      301 ctcgctcttg cgcgtgcgcg cccagcgcgc gagccgctgc ggcgatgcga aatcggcgcc
      361 acgtgccgcc gcatcgccaa ccaggcgacg caacgcagcg acatcgcgca ggcccaggtt
      421 gacgccctgc ccggcgagcg gatgcaccgc gtgcgcagcg tctccgacca gcagcacccg
      481 gccgctgaag taggtttccg ccagttgccg ccgcagtgga aacgccgcgc gcgcggacag
      541 caggcgcatg gttccgagct cgccaccaaa tgcatcgccc agttcgcgcg cgaagccggc
      601 ttcgtcgatg ccgccgaggc gctccgcctc cgcatccggc aaggtccaga cgatggagcc
      661 caggcggcct tgcacggacg cgtcgccgtc gtcggcgaat ggcaggaagg ccactggccc
      721 ggtcggcagg aagcgttgcc agcaggtgtc gcggtgcggc acgccgcttg ccacgaacgc
      781 gaccagcccc ttctgcgcgt agtcgcgctt gcgggtgccg atcccggcca tcccgcgcag
      841 cgcggagttg gcgccgtcgg cggcgatcgc gaccttcgcg tccagcgcca cgccgtcatc
      901 cagctgcagg cgcacgccgg cctccgactg ctcgaccgcc tcgatccgcg ccgggcaatg
      961 caggcgcacc ccggccgccg gcagcgcgtt ccacaacgca tcgaccagca gcgtgttctc
     1021 cacgatccat cccagttgcc gctggccaag cgcatccgca tcgaaggcca gcgcatcgcc
     1081 gccggccgcg tcccacacgc gcatgccgcg gtacggctgc gcccgcgccg ccagcaccgg
     1141 ttgccacacc ccgagatcat cgagcaggca cgcattgtcc ggggcgaagg catacacgcg
     1201 caggtcgcgc gacccgcgct gccaggcggg tgggcgcttc ggctccacca gcgccacctg
     1261 caggccttcg cgcgccagca tcagcgcggt gcacgcaccg accacgccgc caccggcgac
     1321 caccgcatcc agccggtggc ggcggttcat gccgcgccgc cttgccggca cagcgccggc
     1381 acgtcgccgc ggtagcccat cgcgccaccg accagccaac cctgcaacca gcccatcgcg
     1441 tcaccggcga gcaatccggc gctgcgcagc gggcgcagca ggggtgccgc attggaggtc
     1501 agcctggcca ggccatcgga aaacgcgagg gtgcgctcgc ggtcctgcgc ccggcgctgc
     1561 gcatggcggg caagcaatgc cggggcgccg ggatcgccgc catcagcgat gcattcggcc
     1621 agggtcaggg catcgcgcag gcccaggttg aatccctgcg cgccgagcgg gtgcagcgat
     1681 tgcgcggcat tgccgaccag caccgcgcgc ggcgcgacca gttgcgccgc cacgcaaccg
     1741 acgatcggat aggcgctgcg cgggccgcag gcgaggaagc gcccggcgcg ccagccgaag
     1801 gcgtcctgca cgcgcgcgag gaaggtggca tcgtcgagcg cggccaccgc gtcggcgtgg
     1861 tcgcgcgcaa ccccgtgcac caacccgtag tggccatcgc cgcgcggcag cagcgcggtc
     1921 ggcccactgt cggtcaggcg ctcccaggcg gtgccatcgg gcggccgctg ggtgcgcagg
     1981 cgcgccacca acagggtctg tgcatagtcg tgctcgcgcg ccgagatgcc gagtgcgtcg
     2041 cgcaccgagc tgcggctgcc atcggcggcg accagcaggc gcgtgcgcag ttcgcgttcg
     2101 ccgccggcat cgacgatccg caccacccgc tgcgccgggt cggcatcggc atcggccagg
     2161 ccgacgaagc gcgccggacg gtagcgggtc aaccgcgaca gctgcgacag gcgcgtttcc
     2221 agtgcctcgc cgaaatcgcg cgcaaccacg acctggccga atcggtcgcg gccatgcgcg
     2281 cgtgcatcca gccggatgcg gccgaagtcg ccggcgcggc tgacgtggat gcgccggatc
     2341 gcgccggtcg gcgcggccag caacggcatc accccgagcg cagccagcgc attcacggtg
     2401 gcctcggcga agctcaggtt gcgctggtcg aacaccgccg gcaacgcgcc cgcgggggtg
     2461 gcctcgagca tgccgacatc caggccctgg gcatccagcg cgatcgccag gctggcgccg
     2521 accaggccgc cgccgacgat cagcacgtcg tgcaggggca tggtgtcgtt gggggttgcg
     2581 gtcattgcgt tatgctaacg gctccgctgt ccgccgatcg aaacgccatg aatcgccccc
     2641 tgctttcctc cggccccgac ctgcccgccg cgggcccgct ggccctcgcc gcgttgatct
     2701 tcatcgccgc cctgagccgg gtgctgccgc acccgccgaa tttctcgccg atcgaggcgg
     2761 tggcactgtt cggcggcgcc tatttcgcca gccgccgctg ggcgctgctg gtgccactgg
     2821 tggcgatgtt cgcctccgac ctggtgctgg gcctggccaa tggcggcatc tactgggact
     2881 acttccgcag cgccggctac ctgctggtct atgcctgtat cgcgctgtcc acggtgctcg
     2941 gcttcggcct gcgcggcagg gtcagcggca gccgcgtgct gggctattcg ctggccggct
     3001 ccatgctgtt cttcgtggtc accaacttcg gggcctggct gtccgacccg gcctacccga
     3061 agaccatcgc cggcctggtc gcggcctacc tggccggggt gccgttcttc cagtggacgg
     3121 tgctgggcac gctggcctat gccgccctgc tgttcggcgg cttcgaactg ctgcgcaagc
     3181 acgtgcccgc gctgcgggcg cagacggcct gacccgcggc gatgggcaac cgcctgtcga
     3241 agatctacac caggaccggc gacgacggca ccaccggcct cggcgacggc acccgcgtgg
     3301 ccaaggattc ggcgcgggtc aacgcctatg gcacggttga cgaggccaat tcctgcatcg
     3361 gcgtgctgct cgccggcgaa gtgccggatg acgtgcgcgc gctgctgacc cggatccagc
     3421 accagctgtt cgaccttggc ggcgagttgt gcattcccgg gcatgcggcg atcttcgatg
     3481 ccgacatcga tgcgctggag gcaaggctcg accactacaa cgaagggctg ccggtgctga
     3541 aggagttcat cctgcccggc ggcggcgagg cggcggcgcg ttgccacgtc gcccgcaccg
     3601 tggtgcgccg cgcagagcgc gataccgtca ccctctcccg ccacgacgcg gtgcggccgc
     3661 aggcgatccg ctacctcaac cggttgtcgg acctgctgtt cgtgctgtcg cgggtgcttg
     3721 cacgcgccgg cgggcacggc gaggtgctgt gggaacacga ccgccgccga tgagccgcgc
     3781 ttgagcctgc gcatctacac ccatcccgcc tgcctgtggc acgacaccgg ccccgagcac
     3841 gcggagcgac ctgagcgcct gcgcagcgtg atcgatgcgc tgatcgccgc cttccccgaa
     3901 ctcgactggc gcgaggcgcc gcgggccagc cgtggccagt tgctgcgcgc gcacgcgccg
     3961 gacctgctgc aggcggtgct ggagaccaat ccgcgcgagc gcatgctgct ggatccggac
     4021 acggtgctgt cgccggcctc ggcggaagcg gcgctgcgcg ccgccggcgc cggcgtggcg
     4081 gcagtggatg cggtgctggg cgacaccacc acccgtgcct tctgcgcggt ccgcccgccc
     4141 gggcaccacg ccacccacga tgtcgcgatg gggttctgcc tgttcaacag catcgcggtg
     4201 gcggcgttgc atgcgctgga tgcgcacggg ctggagcggg tggcggtggt cgacttcgac
     4261 gtccaccacg gcaacggcac ccaggcgatc ttcgagcgcg atccgcgggt gctgtacgcc
     4321 agctcgcacc agtggccgct gtatccggac agtggcgcgc gctcggaaac cggcgtcggc
     4381 aacatcttca atgcgccgct gccgccggaa acggatggcg cgcggttccg cgccgcgtgg
     4441 tcgggcagcc tgctgccggc gatcgatgcg ttcaggccgc aactgctgct ggtgtcggcc
     4501 gggttcgacg cccaccgccg cgatccgctc gcggaattgc ggctggaggc gggcgacttc
     4561 ggctggatcg cccgcgagct ggtgcaactg gcgaaccgcc actgccgcgg gcgcatcgtg
     4621 tcgatgctgg agggcggcta cgacctcgag gcactggccg agtccagcgt cgcccacgtg
     4681 cgcgagttgc tggcctgatc aggcgccggc agcgtccacc aagaacacca acgaagccga
     4741 gttgacgcaa tagcgccgcc cggtcggtgc cggcccgtcg gcggaccgcg gcaggccgcg
     4801 aactgctgcg gatccaggcg ctggcgccat tcgccttcgg actgcggcag ggcgctggcg
     4861 ggcgggtcct ggccgggctt cgcgggttcc ccgggattcg gccgggccag ccttgacccg
     4921 ggcgggccag ggcgcttcgc ggtttgcccg caccgcgccg ttccggcaag ctagccgttg
     4981 gatcctgtca tctggattgt ccgtgcgccc agccctgcgc ctgctgccgt tgtccctgtg
     5041 catcgccgtc tccctcaacg cccgtgccgg cgacgacatg ccgcagaact ggagcctgtg
     5101 cccggtgcag gacgtggtga agccgttcgc ggaggcgcag gccgccccgg aaggcatcgg
     5161 catcgacaac accgagcagg ccaccgacat cgagggcgat gcgctgaccg gcaccgagga
     5221 aaacccggtg ttcaacggca acgtgaccct gcgccgcggc gaccagttca tgggcgcgga
     5281 ccagctgacc tacgacaagg gccaggagca ctacaccgcc gagggcagca tccgctacca
     5341 gggctccggc ctgcgcatga ccgcagcgcg cgccgagggc gaccaggcca aggacgtcca
     5401 cacaatccat gacctcgact accagttgct ggcgaatcgc ggcaacggcg gcgccgagcg
     5461 cgtgaccctg accggcgaca tcggctcgct gcatggcgca acctattcca cctgcgcgcc
     5521 ggacgcccgc cattgggaac tgcgcgcgaa ccggatcgac atcgataccg agaagggcat
     5581 ggcggtcgcc cgcggcgcca ccctgcgggt cggccaggtg ccgatcatct acctgccctg
     5641 gttcatgttc ccgaccgacg agcgccgccg caccgggctg ttgttcccgt cggtctccaa
     5701 ttccagccgc aacggccttg actggaagca gccgatctac ctcaacctgg ccccgaacta
     5761 cgatgccacc ctcaacccgc ggctgatgac caaccgcggc gtgcagctgg gcgcgcagtt
     5821 ccgctacctg gtcgagggcg gcgccggcgt cgtcgatggc acctggatgc cggacgacga
     5881 cctgcgcgac agcgaccgca gcatgatcag gtacggcgcg ttccagaacg tcaaccgcaa
     5941 ctggcaggcg cgcgccaacc tgaactggat cagcgactcc cgctattacg aagacttcag
     6001 cagcagcctc gacggcctct cgcagtccac ttcgttcagc gagatcggcg tgtacggccg
     6061 cggccacggc tgggaggccg gcatcacctc cgactattgg caactggccg acttcaccct
     6121 gaccgaggcg gacctgccgt acaaccgggt gccgcgggcc tacgtccatt gggaacgcgg
     6181 cctcggcctg cacttcgtcg gcggcgtcga tgccgagatg gtgcggttcc ggcacccgct
     6241 gttcgacgcc ggttcgcgcc tggacgtgaa gccctggatc agcctgccgc tggagggcga
     6301 cagctggttc gtcaagccaa ccgcggcgtt ccgccacacc tcctacgagc tcgatgagag
     6361 cctgaccgcg agccgttcgc gctcgcgcag ccagccgatc ttcagcctcg acagcggcct
     6421 cttcttcgac cgcgacaccc gggtcaaggg caaggactac ctgcagacga tcgaaccgcg
     6481 cctgtactac ctgcacgtgc cctacgccta ccaggacgac atgccgctgt tcgacaccca
     6541 gccgctgacc ttcagctggg gccagctgtt ccgcgacaac cgctattccg gcgccgaccg
     6601 ccaggccgac gccaaccagc tcacgctggc gctgagcacc cgcatgatcc gccagtcgga
     6661 tggccgcgag cgtttctccg ccagcctcgg ccagatccgc tacttcgacg aatcgcgggt
     6721 gagcatcccg ggcgagccgg tcaccgaaaa gggccgttcg gcgtgggtgg cggacgccaa
     6781 ctacgcaccg accgaccgct ggaccttcgg cgcctcctac cagtgggacc cgaaattcaa
     6841 ccgcaccgac ctggccagca tccgcgcccg ctacctgctg ccggacgatg gcgtgttcaa
     6901 cctcggctac cgctatcgcc gctcgctgct ggaacaggcg gatgtttcct tcctgtaccc
     6961 gatcaacccg acctggagcg tcgtcggccg ctactactac tcgatgtacg acagcaaggc
     7021 actggaaacg attgccgggc tgcaatggga cagctgttgt gtcgccgttc ggcttgtggc
     7081 gcgccgctac gtgcacaatc gcgccggcga cctcagcaat tcggtcctgc ttgaaatcga
     7141 actgaagggg ctcggctcgg ccggccagga cacccgtcgc gccttgcgcc gggccatcct
     7201 cggctacaat cgggacgacc tctacctggt gccgccgcag accgccacgg gccagcccac
     7261 cccgcccgcc atcgacacgg ttaacgacgc cagcccatga cactagcccc cacccgctcc
     7321 ctgctcgcca tcgccctcgc cggcctgctg ttcgccgcag gcgcgcatgc gcagagttac
     7381 gccggcaccg cgccggtcga ccgcatcgcc gcggtggtga acgaggacgt gatcctgcgc
     7441 agcgagctgg accgcgcggt cgccaacatc cgcgcacagt acgccggcaa ggaagcccag
     7501 ttgccgccgc aggacgtgct ggaacggcag gtgctggagc gcctggtgct gatgcgcctg
     7561 cagctcgcgc gcgccaccga ttccggcatc accgccagtg acgaggacct cgagcgcgcg
     7621 gtgcagggcg tcgccgaaca gaacggcatg agcgtggacc agttgcgtgc gcggatcagc
     7681 caggacggca tgtccttcac cgagttccgc aacaacctgc gcgatgaaat cgtcacccag
     7741 aagctccgcc agagcttcgc ccagggccgg atcaacgtca gcgaggccga agtggattcg
     7801 gcgatgaccg ccgcggcggc ctccatcagc cagcagttcc gcctggcgca catcctggtc
     7861 ggcaccccgg acggcgcgtc cgccgaacag cttgccaccg cgcagaagaa gatcgaaggg
     7921 gtcaagggcc tgatcgacaa gggcgagatg tccttcgccg ccgccgccgt gcgctattcg
     7981 gacagcccga atgcgctgga gggcggcgac ctcggctggc gcggcctcaa cgagatcccg
     8041 ccggccttcg cgcagaccat ccagcagatg cagcccggcc aggtgatcgg cccgatccgc
     8101 ggcgccagcg gattccagct gctgcagctg gtcgagaccc gcagccaggc cgcgggcggc
     8161 ggcgagcagg tcacccagtt ctccgcccgc cagatcctgg tcaaggtcga cgacacgacc
     8221 gacgacgcgg ccgccaaggc ggaagccgag accctggccg cacgcatcgc tggtggcgca
     8281 gatttcgcca agctggcatc cgaatcctcc gacgacgatg cgaccaagcg ccgcggcggc
     8341 gatctcggct ggttcggcgc agacacctac ggcaatgcgt tcggcatgca ggtcgccgcg
     8401 ctctccgacg gccagacctc ggccccgttc aagaccgatg ccggctgggt gctggtgcaa
     8461 cgcacctcca gccgccaggt catggccggc agcgacagcc tgcgcgcgca ggtgcgggag
     8521 accatcggcc gccgcaagct ggaggacgag tggaaccgct ggacgcgcga gatgcgcggc
     8581 gaagccttcg tcgacgtgcg cggactcgac ggcgcggccg ccaatcccgc taccggcggc
     8641 tgagcgcgcc cccgcagatg cgcccgcggc tcgcgctggt gccgggcgaa ccggcgggga
     8701 tcggcccgga gctggtggtc cgcgcgcttc ggcaggagtt cgacgccgaa ctggtggtct
     8761 tcggtgatgc cgccgcgctc cagcgtgccg cacgcgcact gtcgctgccg ctggaactgc
     8821 atcgcgccgg tgaagcgccg gcgtcgccgc gcagcaccgg gatcgtcgac atcccgcacc
     8881 cgatggcggc cgcgttcggc actcccgaac ccgcgaacgc agcctcggtc gtgcaggcgc
     8941 tgctggcggc ggcgcaggcc tgcgtcgagg gcacttgcca cggcatcgtc accggtccgg
     9001 tgcacaaggc cgcgatcaac gacggcggca tcgccttcac cggcaccacc ggcctgctgg
     9061 ccgcgcacgc cggccgcgag gtggtgatga tgctggccaa cgacatcgtc cgggtcgccc
     9121 tgctgaccgt gcacctgccg ctgcgcgcgg tcgccgacgc ggtcacgccg gcggcgctgg
     9181 cgaccacctt gcgcatcgtc gatgcagcgc tgcgtggcga cttcggcatc gccgagccca
     9241 ccatcgccgt gctcggcctg aacccgcacg ccggcgaagc cgggcacctc gggcgcgagg
     9301 aactggacat catcgagccc acgctgcagc gcctgcgcgg cgagggcttg cgcctggccg
     9361 gcccgctgcc ggcggacacc gcgttcctgc ccgggaagct gcgcggcttc gacgcggtgc
     9421 tggcgatgta ccacgaccag ggcctgccgg tgctgaagta cagcgatttc gcccacgcag
     9481 tgaacatcag cctgggcctg ccctacccgc gggtggcggt cgaccacggc accgcgctgg
     9541 aactggccgg caccggcatg gccgacccgt ccagcctgct cgccgcgatc aacgcctgtg
     9601 cgcgcttcgc acgcaaccgg atgcacgcat gagcacccgc ttcaccgagc cggccaagac
     9661 aagcccctcc ggtttttccg ccgaggcgaa aaagcacctc ggccagaatt tcctgcacga
     9721 caccaccgtc atcgccaaga tcgtgcaggc actggatccc aagccaggcg atgtgatcgt
     9781 ggagatcggc cccggccagg gcgcgctgac cttccccttg ctgcgcaggc acggcgaact
     9841 caccgcgatc gagttcgacc gcgacctgca cgcgccgctg caggccgccg cgcgcgaaca
     9901 cgggaccctg cacctggtcc agggcgacgt gctcggcgtg gacttcagcg cgctggccgc
     9961 ggaacttgcc tgcaccggcg gacagatccg gctggtcggc aacctgccct acaacctcag
    10021 ctcgccgatc ctgttccatg cgctggacca tgccgccgcg gtgcgcgaca tgcacttcat
    10081 gctgcagaag gaggtggtgg agcggatggc cgccggcccc ggcagcaagg tctatgggcg
    10141 cctgagcgtc atgctgcagg cctattgcac ggtcaccccg ctgttcatcg tgccgcccgg
    10201 cgcgttccgg ccggcgccga aggtggagtc ggcggtggtg cggctggtgc cgcggcccgc
    10261 ggccgaggtc gaggtcggcg atcgccgcga cttcgccaac gtggtccgcg ccgccttcgg
    10321 acagcgccgc aagaccctgc gcaacgccct gcacggggtc gccgacagcg ccctgatgga
    10381 agccgccggc gtgcgcccgg atgcgcgcgc cgagcaggtc gaggtcgccg ccttcgttcg
    10441 cctcgccaac ctgctcggcg gcggatgaac gcgcggcggt gcccggccgc gtgcgtcacc
    10501 gctagaatgc acgcatgggc aacaccgact acatcatcga catcgcggtc gacacctgct
    10561 tcctcgacga ccagtccgcg ccggaggaag gccgcttcgt cttcgcctac accatccaca
    10621 tccgcaacca cggcaaggtg cctgcgcgcg tgctcggccg ccattggctg atcaccgaca
    10681 gcaacggcaa ggtgcgcgaa gtggtcggcg agggcgtggt cggcgagcag ccctggctgc
    10741 gtccgggcga gggcttcgag tacacctcgg gcgcggtgct ggagaccgac atcggcacca
    10801 tgcgcggcag ctacgacgtg ctcgccgacg acggcacccg cttcgccgcg ccgatcccgg
    10861 cgttcaccct gtccgtgccg cgcacgctgc actgacgccg ggacccgcgc cgtgagcaca
    10921 tgggccatcg gcgacctgca gggttgccac gacgtcaccc agcgcctgct ggagaagatc
    10981 cgcttcgatc ccgcggtgga ccggctgtgg ttctgcggcg acctggtcaa ccgcggcggc
    11041 cagtcgatcg agaccctgcg cctggtccat tcgctgcgcg cggtgtcgca cgtggtgctg
    11101 ggcaaccacg acctgtcgct gctggcgatc ggcgaacgca gcccggacga acagcgcaag
    11161 gtcaacccgg acctgcaggg cgtgctgttc gccccggacg cgcgcgaact gctcgactgg
    11221 ctgcgcagcc aggaactggc gcatgtcgac cgcgcgctcg gctggatgat ggtgcacgcc
    11281 gggctggcgc cgaagtggac cacccagctt gccgagaagc atgcgcgcga agtcgaggcc
    11341 aagctgcacg gcgacaacta ccgcaagctg ctgaagaaca tgtacggcga cggcccggaa
    11401 tggtcgccgc ggctgaacgg cgccgagcgc gaccgcgcga tcatcaacat cttcacccgc
    11461 atgcgctatt gcagcccgcg cgggcgcatc gccttcgaac acaagggcga acccggcagc
    11521 cagccgcccg gcctgtaccc gtggtattcg gtcccgggcc acgccccgcg cgacctcaag
    11581 gtcgtgtgcg ggcactggag cacgctgggc ctgttcgtcg gccacggcgt gcatgcgatc
    11641 gacaccggcg cggtgtggag cggcaagctc accgcgctgc agctggacag cgaggacctt
    11701 cgcgtggtgc aggtgcccgg ccgcgacgtg ccggcaccgg cgccacgggt gcggccgccg
    11761 aagccgaagc aaccgtcgcc gcagcaccgc cacgacgggc aacgccgcta gctagcgcgc
    11821 ggcgcacgcc tgcggttggt tgcgcgtgta gtcgacgaac gcgaacgcga atgcatgcgc
    11881 tgcatccgcg ggatgcggct ggcgcacgct gaccgtccat tcgcccggat cgaaacgcgg
    11941 gaagaacgcg tcggcgccgg cgaccgcggc atcgacgtgg gtcaggtgca tgtgcgtcgc
    12001 cagcggcagc gccagcgcgt agacctcgcc gccgccgatc acgcacagcg cctccgcgcc
    12061 atcggcggcc gccagcgcct tggcatcggc gatcgaggcc accgcctcca tcccgtcgaa
    12121 cggcacgtgg ccaccgcggg tcagcaccag gttgcgccgc ttcggcagcg cgcgccccag
    12181 cgattgcgcg gtcttgcgcc ccatcagcac cggctggccc agggtcagcg ccttgaaccg
    12241 cttgagatca tccggcaggt gccagggcaa ggcgttgccc ttgccgatgg cgagattgcg
    12301 gtccagcgcg gcgatcaggg cgatgcgcac cgccgcctca gaccgccacc gctgccttga
    12361 tcgccggcag cgggtcgtag ccttcgatcg cgacatcctc gaagcggaag ccgaagatgt
    12421 ccttcacgcc ggggttgagc ttcaacgtcg gcagcgcgcg cggggtgcgc gccagctgct
    12481 cgcgcgcctg ctggaaatga ttgctgtaca ggtgcgcgtc gcccagcgtg tgcacgaaat
    12541 cgcccacgcc cagcccgcag acctgcgcca ccatgtgggt cagcagcgcg tagctggcga
    12601 tgttgaacgg caccccgagg aagatgtcgc cgctgcgctg gtacagctgg cagctgagct
    12661 tgccgttggc cacgtagaac tggaacagcg tgtggcaggg catcagcgcc atcttcggca
    12721 ggtcggccac gttccacgcc gacacgatca gccggcgcga atccgggttg cggcggatct
    12781 cgtccaccac ccagcggacc tggtcgatgg cctgcccgtc cgcgccttcc cagctgcgcc
    12841 actgcttgcc gtagaccggg ccgagttcgc cgttttcgtc ggcccattcg tcccagatcg
    12901 agaccttgtt gtccttcagg taggcggtgt tggtcgcgcc ctgcaggaac cacagcagct
    12961 catggatgat cgagcgcagg tgcagcttct tggtggtgac cagcgggaag cctgcggcca
    13021 ggtcgaagcg catctgccag ccgaacacgc tgcgggtgcc ggtgccggtg cggtccggtt
    13081 tctccgtgcc gtgttccagc acatggccca gcaagtcgag gtattgcttc attgcttgcc
    13141 tccggcaaga cgcctcactt cgcggcctcg gtggcgggct gcggcaccag ggtcggcgag
    13201 gaatgcgatt tccacagcca gaacaaccca agcaggatca gcggcgtgct cagcacctgg
    13261 cccatggtca gccagccgaa ggccaggtaa cccagctgcg catcgggttc gcgcacgaat
    13321 tcgaccagga agcggaagca gccgtacagc aacgcgaaca agcccgacac ggcatggcgc
    13381 ggccgcggcc ggcgcgagta ccagaccagc acgcagaaca tcgccagccc ttccagggtg
    13441 gcctggtaca gctgcgacgg atgccgcgcg aagctgtcga cgatgccgct gtcgaactgc
    13501 gccttcaggg tggcggcatc catcgaggcg aacggctccg gcagcgaacg cgggaacacc
    13561 acgccccagc cgtcgaacgc ggtgccctgg gtcggcttgc cccagagttc gccgccgatc
    13621 cagttgccga tgcggccgaa gccaagtccc gccggcacca gcggggcgac gaaatcgacg
    13681 gtgtcgagga aatgcaggcg ctgccggcgc gaccaccagc cgaccgcgag cagcaccccg
    13741 agcaggccgc catggaagct catcccgccc tcccacacct tcaggatcat cagcgggttg
    13801 gcgatgaagt cagggaagcc gtagaacagg atgtagccga tgcgcccgcc caggaccacg
    13861 ccgagcatcg cgtagaacag caggtcgccg aagccgttgt cgtccacgcc cggcaggcgg
    13921 ccggcgcgga tgcggcggcg gcccagccac caggcgacca ggaacccgag caggtacatc
    13981 accccgtacc agtgcacctg cggatgcacg ctgcccagca gcggcaggtt ccaggtcccg
    14041 aagtcgatgg cgatggggtc gatctggtgg aggtaaggca tgtgcgtatt gtgccgcagt
    14101 ggggcgcgat gcaatccccg tcgcccgcga ccgtcaaagg aacacgggcg cgttcggcag
    14161 ttcgtcctcg tccatgccac ccggtgcctg cgggaaatgc gccgccagca gcgcgctggc
    14221 ggcggcgatg gcgtcgacca ccgcatcggt gtactcgccg gcacgcaggc gctcctccat
    14281 cagcaggcag acgccgcgcc attgttcgtc gcccacccgt ccgcgcaggc cgcggtcggc
    14341 gacgatctcg atgcggtggt cggccagcag caggtacacc agcacgccgt tgttggcggc
    14401 ggtatcccaa acccgcaact gcgcgaaggc gtcgagcgcg cgggtgcgcg cctgcacgcc
    14461 gcgcaggaca tcgcgccagt ccagtgcgcc ctcgaccgcg aagcagacct cgccgcggtg
    14521 gcgggcctcg ccgtcggcga tcgcggcggc gatgcgctgc atgtccgcct caggaaaacg
    14581 gccctgcgca tcgccggtga acaggtgcgc gaacaatcgc ctcatggtgc cggccatcac
    14641 cagccccccg aggcgccacc gcccccggac gtgccgccac cacccgacca gccgcctccg
    14701 ccggaactgc cgccgcccca gccgccaccg ccggaaccgc cgccacccca gccgctgccg
    14761 ccaccgaaac cgccccagtc gtggctgcgg gcgtagcggc gattgccggc gccgggcagc
    14821 aggccgacca ggaacccgat cgccaccccg atcccgccaa ccagcacggc cgacgacagc
    14881 aaccaggcga tgccgccgct ggccgcgccg ccgaacagcg cgcgtacgcc cgccggcgtg
    14941 cgggcgagga ttccccgcac gagctgggcc acgatgaacg cggcgaacag cgcaagcatc
    15001 cagttgtcgc cgccatccgg accggtcgga tgactggcca ccggctccgg cagcggctcg
    15061 ccatcgacca gcttcaccag ctgcgcggtg gcatcgacga tgccgccggc gtagtcgccc
    15121 tggcggaact tcggcaccag gtattcctgg atcacgcgac cggcggtgat gtccgggatc
    15181 gcaccctcca ggccgtagcc gacctcgatc cggaccttgc ggtcgtccct ggccacgacc
    15241 agcagcaggc cgtcatccac gcccctgcgg cccagccgga acgattcgaa tgcacgcacc
    15301 gcgtattgct cgatggtctc cggcgcggtg ctgggaatga ccagcacctg cagctggctg
    15361 cccttgcgct gctgcaggtc cagcgcctgc tgctgcagct ggcgggtggt ggccgggtcc
    15421 agggtgccgg tggtgtcgac caccggcgag tcgaaaggcg ggaccggcgc cggctgctgc
    15481 gccaggcagg caagcggcag ccacgccagc aacgacgcca gtgccagccg ccaaccgcag
    15541 gtgcgcagtg cgtgcatcgg atcgggcact gccggcaggg tcagcgggag gccaggctca
    15601 ctcggtgggc gcgggttgcg gtgcgttgcc gccctgcacc gggggcagtg ccggttgcgg
    15661 cggattgacc accgacgcgg ccggttccac caccggcggc accggcgcgg gctccgccgg
    15721 cgcggcgccg aagtccacgc tcggcgcgtc cgcgatccgc gtctcgtcct cgaccgtgaa
    15781 gttcggcttc tgcttgtagc cgaacaccat cgccaccagg ttctgcggga acgagcggat
    15841 gtaggtgttg tagtcctgca
//
LOCUS       DAORUK010000035        15673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_3513_length_15673_cov_17.942630, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000035 DAORUK010000000
VERSION     DAORUK010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15673
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3513_length_15673_cov_17.942630"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PK818_03835"
     CDS             complement(<1..313)
                     /locus_tag="PK818_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009392033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY81899.1"
                     /translation="MSQFIYTMQGVSKTVPPKRQIIKDISLSFFPGAKIGLLGLNGAG
                     KSTVLRIMAGVDTDFTGEARPQPGIKVGYLEQEPRLNPEQTVREAVEEGVGEVLQAQA
                     RL"
     gene            474..1724
                     /gene="glyA"
                     /locus_tag="PK818_03840"
     CDS             474..1724
                     /gene="glyA"
                     /locus_tag="PK818_03840"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011260263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HQY81900.1"
                     /translation="MFSRADLIAGFDPELAKAIADETRRQEDHVELIASENYASPRVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDVAERLAIERCKQLFGADYANVQPHSGSQAN
                     QAVYFALLQPGDTILGMSLAHGGHLTHGAKVNLSGKLFNAVQYGVNDAGLIDYDEVER
                     LALEHRPKMVVAGFSAYSQVVDWARFRAIADKVGAYLFVDMAHVAGLVAAGVYPNPVP
                     FADVVTSTTHKTLRGPRGGIIVARANEEIEKKLQSIVFPGIQGGPLMHVIAAKAVAFK
                     EALEPEFKAYQEQVVKNAQAMAKTIIARGYKIVSGGTQNHLMLVDMIGKDVSGKQAEE
                     ALGRAHITVNKNSVPNDPRKPFVTSGLRIGTPAVTTRGYKEADCVALAEWICDVLDNP
                     NDDNVIAGVRGNVERQCRQFPVYG"
     gene            1735..2235
                     /locus_tag="PK818_03845"
     CDS             1735..2235
                     /locus_tag="PK818_03845"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQY81901.1"
                     /translation="MRVLHVAAVLASCGMPMPAIAQDEATWASRLDYTLNESRDKPIV
                     CKQGDIERHAGQTLAQVFAEKWPVQPEPLPGSERVQAQATRAIRPRGAMRGMPVQPGI
                     VVFAVLVDENGEPVQAEVLCASTEGYDKYAMRIALQSQYKPAFVNGKPVTSVVVHVQK
                     FAGGAG"
     gene            2239..2757
                     /gene="nrdR"
                     /locus_tag="PK818_03850"
     CDS             2239..2757
                     /gene="nrdR"
                     /locus_tag="PK818_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HQY81902.1"
                     /translation="MHCPFCQHTDTRVIDSRVSEDGATIRRRRGCEACGERFSTLETI
                     ELKLPAIIKSDGRREAFDARKLRAGFDRALQKRPVAEEQIESAVRAVVHAVRMAGERE
                     LASRRVGEFVMAELRKLDHVAYVRFASVYRSFEDVADFREEIERLERELPAGEGQLPL
                     LGAPPAEKGRRR"
     gene            2765..3862
                     /gene="ribD"
                     /locus_tag="PK818_03855"
     CDS             2765..3862
                     /gene="ribD"
                     /locus_tag="PK818_03855"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014506641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HQY81903.1"
                     /translation="MSFSATDHAMMARALRLAERAAYTTRPNPMVGCVLAHGDEVVGE
                     GWHQRKGGPHAEVFAVQAAGDRARGATAYVTLEPCAHSGSTGPCAEALAAAGIARVVG
                     AMRDPFPQVDGAGFEALRAAGIAVECGLLEAQAREMNRGFLSRIERGRPWLRVKLAMS
                     LDGRTALASGDSKWISGEPARRDVHQWRARSGAILTGAGTLLADDPQLTVRLQDDIDV
                     VPPLRVVLDPGLATVARARVREGDAPTLYLHAPDAKPPRGMAVDSAVVPVQDGRFDLL
                     AVLRLLAARQVNEVQVEAGATLAGAFLAAGLVDELLLYIAPVLLGAHARPLFDGLEID
                     AMAQRLRLQTVDSRRIGEDLRLLLRPLAEAA"
     gene            3862..4464
                     /locus_tag="PK818_03860"
     CDS             3862..4464
                     /locus_tag="PK818_03860"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HQY81904.1"
                     /translation="MFTGLIAGVGRLAAREMRGGDARILVEAGTLPFDDVQLGESIAV
                     NGCCLTVVAFDATGFAVDASNETLALTTLGRLAIGAPVNLERAMLPTDRLGGHLVSGH
                     VDGLGIAVKRWDDARAERWRFEAPMALLRYVAHKGSVCVDGVSLTVNAVDDTGFEVAL
                     IPHTVAHTAFHALREGDAVNLEVDLLARYVERLLASKDKA"
     gene            4461..5567
                     /gene="ribB"
                     /locus_tag="PK818_03865"
     CDS             4461..5567
                     /gene="ribB"
                     /locus_tag="PK818_03865"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008576784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HQY81905.1"
                     /translation="MSFASIPELLDELRAGRMVVIVDDEDRENEGDLIMPAELVRPAD
                     INFMVTHARGLVCLSLTRERCAQLGLNPMVRDNTSQHHTNFTVSIEAAEGVTTGISAY
                     DRAHTVRTAVRPDAKAHDLSQPGHIFPLMAQPGGVLTRAGHTEAASDLALLAGFEPAG
                     VLVEILNGDGSMARRPELEVFAREHGLKIGSIEDLIRHRLATEHTVERVDSRDIGTDH
                     GGFVLHCYRDRLTRGLHYALVRGEVAGDAPVLVRVQPQNPLADALHWRRDDFGPLVGD
                     VLAMIAAEGRGVLVLLGDAHDAEATLARIREQPVAPASRAGTLAQWRRTGAGSQILSD
                     LGLHRLRVLGTPRRQVGLAGFGLEVVEYAALPPR"
     gene            5597..6076
                     /gene="ribH"
                     /locus_tag="PK818_03870"
     CDS             5597..6076
                     /gene="ribH"
                     /locus_tag="PK818_03870"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HQY81906.1"
                     /translation="MPYFEGDLRAPEGARFAIIASRWNPRITDALVAAARQAFVGNGV
                     DEAALDVVRVPGAWELPVAARQLAVAGRHAAIVALGCVVRGDTRHYEQVADGTSEGLM
                     RVALDTGVPVANGVLAVERFEDASARAGGSHGNKGEEAALVAIEMADLCRKLQESAR"
     gene            6073..6528
                     /gene="nusB"
                     /locus_tag="PK818_03875"
     CDS             6073..6528
                     /gene="nusB"
                     /locus_tag="PK818_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQY81907.1"
                     /translation="MNHRRRADGIDPVARSRSRRRALQAIYAWQINHADERGLIAQFA
                     HEQAHEAADLEYFEDLVRGVLRHVGELDAALAGHLDRGVDEVDAIERAALRIAAYELI
                     HRPDVPYRVVINEAIDSTKRFGSEHGHTYVNGVLDKAAAALRSTEAHAR"
     gene            6542..7531
                     /gene="thiL"
                     /locus_tag="PK818_03880"
     CDS             6542..7531
                     /gene="thiL"
                     /locus_tag="PK818_03880"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HQY81908.1"
                     /translation="MGEFDLIARIRARAPTRADVLLGIGDDAALLLPPAGLQLVVAMD
                     TLNAGVHFPPATAPADIGWKALAVNLSDLAAMGAQPAWCTLSLSMPEAQAGFVDGFLD
                     GFLALAAQYDVALVGGDTTRGPLSVCVAVHGLVEPGMALRRDGARAGDEVWVSGSVGD
                     AAAALVQWKEGCKGGQLGCPAEAQPDPLSLALRARLDRPTPRVALGRALRGVASACID
                     VSDGLLADLGHVCAASGVAATVDVDALPASEALRASFSSERRRHLQATGGDDYELCFS
                     AAADAHAVVLRAAATAAVAVTRIGRILPGAGVAAHTADTDPWSAPATGWVHFA"
     gene            complement(7496..8347)
                     /locus_tag="PK818_03885"
     CDS             complement(7496..8347)
                     /locus_tag="PK818_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQY81909.1"
                     /translation="MRMLLLAAWLVGTSLMPVHAQTDATPAREYRVLLVGNSLTYTNN
                     LPALLRAVGASQGTPIATESFAAPGGTLIERWNDGHVAEALRSRGFDAVVLQEQGGNL
                     AACMASVQQQRKAPCAASLRAYTEFAQLATVKGAKVLVFATWGPDERWQGKLARSARM
                     IAESASATVFDAAGALQALHKAQPGINLFPDGNHPSTQASLMLALALYRDITGSAPVA
                     KDLRVTAPLLPVNAAVSPASAMESQPGLAGDGKAVVVPASLVEPLVQALPGPGSSSEM
                     DPAGRRR"
     gene            complement(8450..8839)
                     /locus_tag="PK818_03890"
     CDS             complement(8450..8839)
                     /locus_tag="PK818_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007968683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HQY81910.1"
                     /translation="MAGADRPLDRRARGDAVEAAALGFLQAQGLRLLARNARARGGEI
                     DLVMQDGDAVVFVEVRFRAGGGFGGGAASVDAGKRRKLIRAAQVFLLRHPRHADAPCR
                     FDVVAACGDPDAPRLDWLRDAFRADDT"
     gene            complement(8826..10163)
                     /locus_tag="PK818_03895"
     CDS             complement(8826..10163)
                     /locus_tag="PK818_03895"
                     /inference="COORDINATES: protein motif:HMM:NF016251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HQY81911.1"
                     /translation="MARKHLVGRFTRSLCCALFLLALAACASVEVDRTPVATMHPATA
                     EAQALARAHAGLPGPARAGNAARIEALLAQLDDASLARDAGALAVGDPLYNFAGRALL
                     NRGLSLPRPFDRGEWNFDAAGRPPADRDGYRPPMRVALLLPLTGGQAAAATAVRDGFL
                     TGYYGEGRRRPEVRFYDSASGANAAYARAVADGNDFVVGPLAREEVDAVFANDTPPVP
                     LLALNRGSRMPPAGNASFSLSPEDEGISLAQYLVDRGARRVLIASGGEDAQRRSAGAA
                     KAQLERRGVQATEEMTGAQGVDAVLLAMKAPQARALLARLAAAGVVVATRVATAQITS
                     GTGKAGEDAALDGIVFPTEAWAARNVAGLPAQARAAERLGTAKGPAARLFAFGFDAWL
                     LTAYLERLALSANADIAGATGQLSLDGFGNVLRRPSWSRFAGGVPVPLDDGGR"
     gene            10224..11054
                     /gene="rsmI"
                     /locus_tag="PK818_03900"
     CDS             10224..11054
                     /gene="rsmI"
                     /locus_tag="PK818_03900"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HQY81912.1"
                     /translation="MQRPGTLFVVATPIGNLGDLSPRALDTLKKVSAVCAEDTRHTRQ
                     LLSHFGVDRPLVALHEHNEGDAAGPLVARLLAGESLALVSDAGTPLVSDPGFRLVRAA
                     REAGIAVSPVPGASALVAALSVAGLPSDRFIFEGFLPAKANARRERLAMLAAEPRTLI
                     FYESAHRIEDMLADAAAAFGAERRAVVARELTKLFETVLDGSLADLAQRVRADPNQRK
                     GEFVLLVHGAGEAADAKVTEGRRLYAKLSEHLPPSTAAKVAAEWSGAPRKALYGGGNE
                     "
     gene            11077..12192
                     /locus_tag="PK818_03905"
     CDS             11077..12192
                     /locus_tag="PK818_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HQY81913.1"
                     /translation="MSKTISRRGFIQAGAGLAAAGVLPAASAQAPAVRGGKPKPVVIA
                     SGNGHQFRNGGRLTCVETAFEQVVAGKDVLDAVIAGVNIVELDPADTSVGYGGLPNAD
                     GVVQLDSCCMHGPLRRAGGVAALEGVRTPSMVARAVLRETDHHLLVGAGAQTFARQLG
                     FAIEDDLNTDTSRRLWLEWKRRVDPTHFLDPASRAQASLRAGQSMVADGLVDPEHFYG
                     TINCSAINAAGDICGVTTTSGLAWKIPGRVGDSPILGAGLYLDNDVGAAGSTGRGEAN
                     LYNLTSHLIVENLRRGMHPKDAAIDGLRRIVANTVEKRLRNARGLPNFGISFYVLDKQ
                     GRHAGVSMYAGGKYAVCDDNGGRQVVMDALLDGKPGA"
     gene            12237..12608
                     /gene="rnpB"
                     /locus_tag="PK818_03910"
     ncRNA           12237..12608
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK818_03910"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(12643..13401)
                     /locus_tag="PK818_03915"
     CDS             complement(12643..13401)
                     /locus_tag="PK818_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HQY81914.1"
                     /translation="MCVVAFACNAHPRWRLLLAGNRDEFHARPTAAVARWPDSGLLAG
                     RDLQSGGTWVGLDTRGRVAVVTNVRDGFAKPHDGPSRGALPHAFLAGSADAAGTTDAL
                     LAGAHAYAPFNLMLADAGGCWHLGNHPRQRDVLAAGVHGISNGRLDAPWPKTRRLVAA
                     LQGWIDLGSDDLQAVWDALADEHVAADADLPATGVGIDLERRLSPTFIRGETYGTRAS
                     TVIAVARDGRGFIHERRFGPHGVFEGESVLRNYA"
     gene            13733..14179
                     /gene="mraZ"
                     /locus_tag="PK818_03920"
     CDS             13733..14179
                     /gene="mraZ"
                     /locus_tag="PK818_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HQY81915.1"
                     /translation="MFQGETAISIDDKGRLAIPTAYRDLVARECGNQLVLTYSPFEEG
                     CLYLYPLPVWEKVRDQVNALPRTKKNSRLLQLKLVGAAAFAEPDGSGRISIPASQRSA
                     VGIDRKAVLLGMGDKFELWSEQAHHAQIRQTLGDADMGDDLQDLVL"
     gene            14176..15123
                     /gene="rsmH"
                     /locus_tag="PK818_03925"
                     /pseudo
     CDS             14176..15123
                     /gene="rsmH"
                     /locus_tag="PK818_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
     gene            15150..15416
                     /gene="ftsL"
                     /locus_tag="PK818_03930"
     CDS             15150..15416
                     /gene="ftsL"
                     /locus_tag="PK818_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HQY81916.1"
                     /translation="MNRYLVLAVLVAANMATALLVVRDRHEHRLAFVALSKLEKARDD
                     LNIEFGRLQLEQATWAESNRVDQVARSRLGMVFPRTEDIVVLRR"
     gene            15413..>15673
                     /locus_tag="PK818_03935"
     CDS             15413..>15673
                     /locus_tag="PK818_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011260235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81917.1"
                     /translation="MKLPGLRLPNLQKRDPARRDGVRAPAGNRPRGRTQFNLRGRLLL
                     VVGTLALCSVALLARALDLQVVDKAFYQQQGAARFLREIPIAT"
BASE COUNT         2353 a         5353 c         5739 g         2228 t
ORIGIN      
        1 ccagccgggc ctgcgcctgc agcacttcgc ccacgccttc ctcgaccgct tcgcggacgg
       61 tctgttcggg gttcaggcgc ggttcctgtt ccaggtagcc aaccttgatc ccgggctgcg
      121 ggcgggcttc gccggtgaaa tcggtgtcga cgccggccat gatccgcagc acggtggact
      181 tgccggcacc gttcaggccg agcaggccga tcttggcgcc cgggaagaac gacaacgaga
      241 tgtccttgat gatctggcgc ttcggcggga cggtcttgct gacgccctgc atggtgtaga
      301 tgaattgcga catcggaaaa actccgcagg cgggccgcgg cgctgcgcgt ggcagggccg
      361 cgaaaggggg tctggaatgg cgaaattata gctggaacgc cttgttccat cgaagccgga
      421 ccggcaaagc cgctaaaatc gggatcccca cctgcccgcc ccggagctgc cgaatgttct
      481 cccgtgccga cctgattgcc ggtttcgacc ccgaactcgc caaggccatc gccgacgaga
      541 cccgccgcca ggaggaccac gtcgagctga tcgccagcga gaactacgcc agcccgcggg
      601 tgatggaagc gcagggcagc cagctgacca acaagtacgc cgagggctac ccgggcaagc
      661 gctattacgg cggttgcgaa tacgtcgacg tggccgagcg gctggcgatc gaacgctgca
      721 agcagctgtt cggtgccgac tacgcgaacg tgcagccgca ttccggctcg caggccaacc
      781 aggcggtgta tttcgcgctg ttgcagccgg gcgacaccat cctcgggatg tcgctggcgc
      841 acggtggcca cctgacccat ggcgccaagg tcaacctcag tggcaagctg ttcaacgccg
      901 tgcagtacgg cgtcaacgat gccggcctga tcgattacga cgaagtcgag cggctggcgc
      961 tggagcacag gccgaagatg gtggtggccg gtttctccgc gtactcgcaa gtggtcgact
     1021 gggcgcgctt ccgcgccatc gccgacaagg tcggtgcgta cctgttcgtg gacatggcgc
     1081 atgtcgccgg cctggtcgcc gccggcgtgt atccgaaccc ggtgccgttc gccgacgtgg
     1141 tcacctccac cacccacaag accctgcgcg gcccgcgcgg cggcatcatc gtggccaggg
     1201 ccaacgagga gatcgagaag aagctgcaga gcatcgtctt cccgggcatc cagggcggcc
     1261 cgctgatgca cgtgatcgcg gccaaggcgg tggcgttcaa ggaggcgctg gagccggagt
     1321 tcaaggccta ccaggagcag gtggtgaaga acgcgcaggc gatggcgaag acgatcatcg
     1381 cgcgcggcta caagatcgtc tccggcggca cccagaacca cctgatgctg gtcgacatga
     1441 tcggcaagga cgtgagcggc aagcaggccg aggaagcact gggccgcgcg cacatcaccg
     1501 tcaacaagaa ctcggtgccg aacgacccgc gcaagccctt cgtcacctcc ggcctgcgca
     1561 tcggcacccc ggcggtcacc acccgcggct acaaggaagc cgattgcgtg gcgctggcgg
     1621 agtggatctg cgacgtgctc gacaacccga acgacgacaa cgtgatcgcc ggcgtgcgcg
     1681 gcaacgtgga gcggcagtgc cggcagttcc cggtgtacgg atgaggatca agtgatgcgt
     1741 gttctgcatg ttgccgcggt gctggcgagc tgtggcatgc cgatgccggc cattgcgcag
     1801 gacgaggcca cgtgggcgtc caggctcgac tacaccctca acgagtcgcg tgataagccg
     1861 atcgtatgca agcagggcga tatcgagcgc cacgccgggc agacgctggc gcaggtgttc
     1921 gcggagaagt ggccggttca gccggagccc ttgccgggta gcgagcgggt gcaagcgcaa
     1981 gcgaccaggg cgatacgccc ccggggtgcg atgcggggca tgcccgtgca gcccggcatt
     2041 gtcgttttcg cggtgctggt ggatgaaaat ggcgagccgg tgcaggcgga agtcttgtgc
     2101 gcgagcaccg agggttacga caaatacgcg atgcgaatcg cattgcagtc ccagtacaag
     2161 ccagcctttg tcaatggaaa gccagtgacc agcgttgtcg tgcacgtgca gaaattcgcc
     2221 ggtggtgcgg gctgagccat gcattgcccc ttctgccaac acaccgacac gcgcgtgatc
     2281 gattcgcgcg tcagcgaaga cggcgccacg atccgccggc gccgcggctg cgaagcctgc
     2341 ggtgagcgtt tcagcacgct ggaaaccatc gagctgaagc tgccggcgat catcaagtcc
     2401 gatggtcggc gggaggcgtt cgatgcccgc aagctgcgcg ccggtttcga ccgcgcgctg
     2461 cagaagcgtc cggtggccga ggagcagatc gaatcggcgg tgcgcgcggt ggtgcacgcg
     2521 gtgcgcatgg ccggcgagcg cgaactggcc tcgcgccggg tcggcgaatt cgtgatggcg
     2581 gagctgcgca agctggacca cgtggcctac gtgcgcttcg cctcggtgta ccgcagcttc
     2641 gaggacgtcg ccgatttccg cgaggagatc gagcggctcg agcgcgagct gccggcgggc
     2701 gaagggcagc tgccgctgct gggcgcgcca ccggccgaga agggccggcg caggtgaccg
     2761 cggcatgagt ttctccgcca ccgaccacgc gatgatggcg cgcgcgctgc gcctggccga
     2821 acgcgcggcc tacaccaccc ggccgaaccc gatggtcggt tgcgtgcttg cgcacggcga
     2881 cgaggtcgtc ggcgagggct ggcaccagcg caagggcggg ccgcacgcgg aggtgttcgc
     2941 ggtgcaggcg gcgggcgatc gcgcccgcgg cgccaccgcc tacgtcaccc tcgagccctg
     3001 cgcgcacagc ggcagcaccg ggccgtgcgc ggaggcgctg gcggcggcgg gcatcgcccg
     3061 cgtggtcggc gcgatgcgcg atccgttccc gcaggtcgat ggtgccgggt tcgaggcgct
     3121 gcgcgcggcc gggatcgcgg tcgagtgcgg cctgctggaa gcgcaggcgc gcgagatgaa
     3181 ccgcgggttc ctgtcgcgca tcgagcgcgg ccggccgtgg ctgcgggtca agctggcgat
     3241 gagcctggac ggccgcaccg cgctcgcgtc gggcgattcc aagtggatca gcggcgaacc
     3301 ggcgcgccgc gacgtgcacc aatggcgcgc gcgcagcggc gcgatcctca ccggcgccgg
     3361 caccctgttg gccgatgatc cgcagctcac cgtgcgcctg caggacgata tcgacgtggt
     3421 gccgccgctg cgggtggtgc tggaccccgg gcttgccacc gtcgcccgcg cgcgggtgcg
     3481 cgaaggcgat gcgcccaccc tgtacctgca tgcccccgat gccaagccgc cgcgcgggat
     3541 ggcggtggac agcgcggtgg tgccggtgca ggacgggcgc ttcgacctgc tggcggtgct
     3601 gcgcctgctg gccgcgcgcc aggtcaacga ggtccaggtc gaggcgggtg cgacccttgc
     3661 cggggcgttc ctggccgcgg gcctggtcga cgaactgctg ctgtacatcg cgccggtgct
     3721 gctgggcgcg cacgcgcggc cgctgttcga cgggctggag atcgatgcga tggcgcagcg
     3781 cctgcgcctg cagacggtgg acagccggcg gatcggcgag gacctgcggc tgttgctgcg
     3841 cccactggcg gaggcggcct gatgttcacc gggctgatcg ccggcgtcgg ccgcctggcc
     3901 gcacgcgaaa tgcgcggcgg cgacgcgcgg atcctggtcg aggccggcac cctgccgttc
     3961 gacgacgtgc agctcggcga gagcatcgcc gtcaacggct gctgcctgac cgtggtcgcg
     4021 ttcgacgcca ccggcttcgc ggtggacgcc tccaacgaga cgctggcgct gaccaccctc
     4081 ggccggctcg cgatcggcgc gccggtcaac ctggaacgcg ccatgctgcc caccgaccgc
     4141 ctcggcgggc acctggtcag cggccatgtc gatggactcg gcattgccgt gaagcgctgg
     4201 gacgatgcgc gcgccgagcg ctggcgattc gaggcgccga tggcgctgct gcgctacgtc
     4261 gcgcacaagg gatcggtgtg cgtggacggg gtcagcctga ccgtcaatgc cgtcgacgac
     4321 accggcttcg aagtcgcgct gatcccgcac accgtcgccc acaccgcgtt ccacgcattg
     4381 cgcgagggcg acgcggtcaa cctggaagtg gacctgctgg cgcgctacgt cgagcggctg
     4441 ctggcaagca aggacaaggc atgagtttcg caagcatccc ggaattgctg gacgaactgc
     4501 gcgccgggcg catggtggtg atcgtcgacg acgaggaccg cgagaacgag ggcgacctga
     4561 tcatgccggc cgaactggtg cggccggccg acatcaactt catggtgacc cacgcgcgcg
     4621 ggctggtgtg cctgtcgctg acccgcgaac gctgcgcgca gctcggcctg aacccgatgg
     4681 tgcgcgacaa cacctcgcag caccacacca acttcacggt cagcatcgag gccgccgagg
     4741 gcgtgaccac cggcatcagc gcctacgacc gcgcgcatac cgtgcgcacc gcggtgcggc
     4801 cggatgcgaa ggcgcacgac ctgtcccagc ccggccacat cttcccgctg atggcccagc
     4861 ccggcggcgt gctgacccgc gccggccata ccgaggccgc cagcgacctg gccctgctgg
     4921 ccgggttcga accggccggg gtgctggtcg agatcctcaa cggcgacggc tcgatggcgc
     4981 gccgccccga gctcgaggtg ttcgcgcgcg agcacgggct gaagatcggc tcgatcgagg
     5041 acctgatccg ccaccggctg gcgaccgaac acacggtgga gcgggtggac agccgcgaca
     5101 tcgggaccga ccacggcggt ttcgtgctgc actgctaccg cgaccgcctg acccgcggcc
     5161 tgcactacgc gctggtgcgc ggcgaggtcg ccggcgacgc cccggtgctg gtgcgggtgc
     5221 agccgcagaa cccgctggcc gacgccctgc actggcggcg cgacgatttc ggcccgctgg
     5281 tcggcgacgt gctggcgatg atcgccgccg aaggccgcgg cgtgctggtg ctgctgggcg
     5341 atgcccacga tgccgaggcg accctggcgc ggatccgcga gcagccggtg gcgccggcct
     5401 cgcgggcggg gacgctggcg cagtggcggc gcaccggtgc cggctcgcag atcctgtccg
     5461 acctcggcct gcaccggttg cgcgtgctcg gcaccccgcg ccggcaggtc gggctggccg
     5521 ggttcgggct ggaagtcgtc gaatacgcgg cgctgccgcc gcgctaaact gcacggcccc
     5581 gcagcgaacc gtgcccatgc cgtatttcga aggcgacctg cgtgcccccg agggcgcgcg
     5641 cttcgcgatc atcgccagcc gctggaaccc gcgcatcacc gatgcgctgg tggccgcggc
     5701 gcgccaggcc ttcgtcggca acggcgtgga cgaggcggcg ctggacgtgg tccgggtgcc
     5761 cggcgcctgg gaattgccgg tggccgcgcg ccagctggcg gtggccggcc ggcatgccgc
     5821 gatcgttgcc ctgggctgcg tggtgcgtgg cgacacccgc cattacgagc aggtcgccga
     5881 cggcacctcc gaggggctga tgcgggtcgc gctcgacacc ggcgtaccgg tggcgaacgg
     5941 ggtgctggcg gtcgagcgct tcgaggacgc cagtgcgcgc gccggcggca gccacggcaa
     6001 caagggcgag gaggccgcgc tggtcgccat cgaaatggcc gacctgtgcc gcaagttgca
     6061 ggagagtgcg cgatgaacca ccgccgccgt gccgatggca tcgaccccgt ggcccgctcg
     6121 cgttcgcgcc ggcgcgccct gcaggcgatc tacgcctggc agatcaacca cgccgacgaa
     6181 cgcgggctga tcgcccagtt cgcccacgaa caggcgcacg aagccgccga cctcgagtac
     6241 ttcgaggacc tggtgcgcgg cgtgctgcgc cacgtcggcg aactcgacgc ggccctggcc
     6301 gggcacctgg accgcggggt ggacgaggtc gacgcgatcg agcgcgcggc gctgcggatc
     6361 gccgcctacg aactgatcca ccggccggac gtgccgtacc gggtggtgat caacgaggcg
     6421 atcgattcga ccaagcgctt cggctccgag cacggccaca cctacgtcaa cggcgtgctc
     6481 gacaaggccg ccgccgcgct gcgcagcacc gaggcgcacg cgcgctgacc tgcgcgcagg
     6541 catgggcgaa ttcgacctga tcgcgcgcat ccgcgcccgc gcgccgaccc gcgccgacgt
     6601 gctgctcggg atcggcgacg acgccgcgct gctgctgcca cccgccggcc tgcagctggt
     6661 ggtggcgatg gacacgctca acgcgggcgt gcacttcccg ccagcaaccg cgccggccga
     6721 catcggctgg aaggcgctgg cggtgaacct gtccgacctt gccgcgatgg gcgcgcagcc
     6781 ggcgtggtgc acgctgtcgc tgtcgatgcc ggaggcgcag gccgggttcg tcgacgggtt
     6841 cctggacggc ttcctggcac tggcggcgca atacgatgtc gcgctggtgg gcggcgatac
     6901 cacccgcggc ccgctgtcgg tgtgcgtggc ggtgcacggg ctggtcgaac ccggcatggc
     6961 cctgcgccgc gatggcgcgc gcgccggcga cgaggtctgg gtcagtggca gcgtaggcga
     7021 tgcggcggcg gcgcttgtgc aatggaaaga gggttgcaaa ggaggtcagc ttgggtgtcc
     7081 tgcggaagcc caacccgacc ccctttcact cgccttgcgc gcgcggctgg accggcccac
     7141 gccgcgggtg gcgcttgggc gcgccctgcg cggggtggca agtgcgtgca tcgacgtttc
     7201 cgacggactg ctcgccgacc ttggccacgt ctgcgctgcc agcggtgttg ccgcgacggt
     7261 cgatgtcgat gcgttgccag cctcggaggc cttgcgcgcg agcttttcga gcgagcgccg
     7321 gcggcacctg caggccacgg gcggcgacga ctacgagctg tgcttcagcg ccgccgcgga
     7381 tgcgcacgcg gtggtgctgc gggcggcggc aaccgccgcg gtggcggtga cgcggatcgg
     7441 acggatcctg cccggtgccg gcgttgcggc gcataccgcc gataccgacc cgtggtcagc
     7501 gccggcgacc ggctgggtcc atttcgcttg aggacccggg ccccggcagg gcctgcacca
     7561 gcggttcgac caggctggcg gggaccacca cggccttgcc gtcgccggca agccccggct
     7621 gcgactccat cgccgatgcc ggcgagaccg ccgcgttcac cggtagcagc ggcgcggtga
     7681 cgcgcagatc cttcgccacc ggcgcgctgc cggtgatgtc gcggtagagc gccagcgcca
     7741 gcatcaagga cgcctgcgtg ctggggtggt tgccgtccgg gaacaggttg atgcccggct
     7801 gcgccttgtg cagcgcctgc agggcgcccg cggcatcgaa cacggttgcg gacgcgctct
     7861 ccgcgatcat gcgtgcgctc cgggccagct tgccctgcca gcgctcgtcc ggtccccagg
     7921 tggcgaacac cagcaccttc gcccccttga ccgtggccag ttgcgcgaat tccgtgtacg
     7981 cccgaaggct ggcggcgcag ggcgccttgc gctgttgctg cacgctggcc atgcaggcgg
     8041 ccaggttccc gccctgttcc tgcaggacca ccgcgtcgaa gccgcggctg cgcagggctt
     8101 cggcgacatg gccgtcgttc cagcgttcga tcagcgtgcc gcccggcgcg gcgaaactct
     8161 cggtggcgat cggggtgccc tgcgacgcac cgaccgcgcg cagcagcgcg ggcaggttgt
     8221 tggtgtaggt caggctgttg ccgaccagca gcacccggta ttcgcgggcg ggggttgcgt
     8281 ccgtctgcgc gtgcaccggc atcaggctgg tgcccaccag ccatgccgcc agcaggagca
     8341 tccgcatcgc catcatgggc ttggtccggg aatcgggttt acggaagctt aagcgtctag
     8401 cggcctggcc gcccatgccg ttggttggcc tgcacccgcg gagacccgct caggtgtcgt
     8461 cggcacggaa cgcatcgcgc agccagtcca accgcggcgc gtccgggtcg ccgcaggcgg
     8521 cgaccacgtc gaagcggcag ggcgcatccg catgccgcgg atggcgcagc aggaagacct
     8581 gggcggcgcg tatcagcttg cggcgcttgc cggcatccac cgaggccgcg ccgccaccga
     8641 aaccgccgcc agcgcggaag cgcacttcga cgaagaccac ggcatcgccg tcctgcatca
     8701 ccaggtcgat ctcgccgccg cgcgcgcgtg cattgcgcgc gagcaggcgc aggccctgcg
     8761 cctgcaggaa tccaagcgca gcggcttcca ccgcatcgcc gcgggcgcgg cggtccagtg
     8821 ggcggtcagc gcccgccatc gtccagcggc accgggacgc cgccggcaaa gcgcgaccag
     8881 ctcggccgcc gcaacacgtt gccgaagccg tccaggctca gctggccggt ggcgccggcg
     8941 atgtcggcat tggcggacag cgccagccgc tcgaggtagg cggtcagcag ccacgcgtcg
     9001 aagccgaacg cgaacagccg tgccgccggg cccttcgcgg tgccaagccg ttccgcggcg
     9061 cgcgcctgcg cgggcaggcc ggcgacattg cgcgcggccc aggcctcggt cgggaacacg
     9121 atgccatcca gcgccgcgtc ctcgccggcc ttgccggttc cggaggtgat ctgcgcggtg
     9181 gccacgcggg tggccaccac caccccggct gccgccagcc gcgccagcag tgcgcgcgcc
     9241 tgcggggcct tcatcgcgag cagcactgca tccacgccct gcgcgccggt catctcttcg
     9301 gtcgcctgta cgccacgccg ttcaagctgc gccttcgccg cgccggcgct gcgccgctgc
     9361 gcatcctcgc cgccgctggc gatcagcacg cgcctggccc cgcgatcgac caggtactgg
     9421 gccaggctga tgccctcgtc ctcgggcgac agcgagaagc tcgcattgcc ggccggcggc
     9481 atccggctgc cgcggttgag cgccagcagc gggaccggcg gggtgtcatt ggcgaacacc
     9541 gcgtcgactt cctcgcgcgc cagcgggccg accacgaagt cgttgccgtc ggcaacggcg
     9601 cgcgcgtagg ccgcattcgc gccactggcg ctgtcgtaga agcgcacctc gggccggcgc
     9661 cggccttcgc cgtaatagcc ggtcaggaag ccgtcgcgga ccgcagtcgc ggcggcggcc
     9721 tggcccccgg tcaggggcag cagcagcgcc accctcatcg gcggccggta gccgtcgcgg
     9781 tcggccgggg gacgcccggc ggcgtcgaaa ttccactcgc cgcggtcgaa cgggcgcggc
     9841 agcgacagcc cgcggttgag cagcgcgcgg ccggcgaagt tgtagagcgg gtcgccgacc
     9901 gccagtgcgc cggcgtcgcg ggccaggctg gcatcgtcga gttgtgccag cagtgcctcg
     9961 atccgcgcgg cgttgccggc gcgcgccggc ccgggcagtc cggcatgcgc gcgcgccagc
    10021 gcctgcgctt cggcggtggc agggtgcatc gtggccaccg gcgtgcggtc cacctccacg
    10081 ctggcgcatg cggccagcgc cagcaggaac agggcgcagc acaggctgcg ggtgaatcgg
    10141 ccaaccaggt gtttgcgggc cacggatcgg cgctcgttcg ggaaccggct aggattctac
    10201 ctgcgcccaa ggtaaaggca gtgatgcagc gtcccggaac attgttcgtg gtggccacgc
    10261 cgatcggcaa cctgggcgac ctcagcccgc gtgccctcga cacgttgaag aaggtgtccg
    10321 cggtatgtgc ggaagacacc cgccataccc gccagctgct gtcccatttc ggcgtggaca
    10381 ggccgctggt cgcgctgcat gagcacaacg agggtgatgc cgccggcccg ctggtcgcgc
    10441 ggctgctggc cggcgaatcg ctggcgctgg tgtccgatgc cggcaccccg ctggtcagtg
    10501 acccgggctt ccggctggtg cgcgccgcgc gcgaggccgg gatcgcggtc agcccggtgc
    10561 cgggggcgag tgcgctggtc gcggccctca gcgtggccgg gctgcccagc gatcgcttca
    10621 tcttcgaggg cttcctgccg gcgaaggcga acgcccgccg cgagcgcctg gccatgctgg
    10681 ccgccgaacc tcgcacgctg atcttctacg agtccgcgca tcggatcgaa gacatgctgg
    10741 ccgatgccgc ggctgcattt ggcgcggaac gccgcgcggt ggtggcacgc gagctgacca
    10801 agctgttcga aaccgtgctc gatggcagcc tggccgacct cgcgcaacgc gttcgtgcgg
    10861 atccgaacca gcgcaagggc gagttcgtgc tgctggtgca tggcgcaggc gaagccgcgg
    10921 atgccaaggt caccgagggc cgccgcctgt acgcgaaact gagcgaacac ctgccgccct
    10981 ccacggcggc gaaagtggca gcggaatggt cgggcgcgcc gcgcaaggcc ttgtacgggg
    11041 gcggcaacga atgacacagc caacggggat gcaaccatga gcaagacgat ttcaaggcgc
    11101 ggtttcatcc aggcaggcgc agggcttgcc gcggcagggg tgctgccggc ggcatcggcg
    11161 caggcgccgg cggtccgcgg cggcaagccg aagccggtgg tgatcgcctc cggcaatggc
    11221 caccagttcc gcaatggcgg caggctgacc tgcgtcgaga ccgcgttcga gcaggtcgtg
    11281 gccggcaagg acgtgctgga tgcggtgatc gccggggtca acatcgtcga actcgatccg
    11341 gccgatacca gcgtcggcta cggtggcctg ccgaatgcgg atggcgtggt gcagctggat
    11401 tcctgctgca tgcacgggcc attgcggcgc gccggcgggg tggccgcgct ggaaggcgtg
    11461 cgcacgccgt cgatggtcgc gcgcgcggtg ctccgggaaa ccgaccacca cctgctggtt
    11521 ggcgccggcg cgcaaacctt cgcgcggcag ctgggcttcg ccatcgagga cgacctcaat
    11581 accgatacct cgcgcaggct gtggctggaa tggaagcggc gggtcgatcc cacccatttc
    11641 ctggacccgg cctcgcgcgc gcaggcgtcg ttgcgcgccg gccagtcgat ggtggccgat
    11701 ggcctggtgg atcccgaaca cttctacggc accatcaatt gctccgccat caacgcggcg
    11761 ggcgacatct gcggggtcac caccaccagc gggcttgcgt ggaagatccc gggccgcgtc
    11821 ggcgattcgc cgatcctcgg cgccggcctc tacctggaca acgatgtcgg cgccgccggc
    11881 agcaccgggc gcggcgaggc caacctgtac aacctcacct cgcacctgat cgtcgaaaac
    11941 ctgcgccgcg gcatgcaccc gaaggacgcg gcgatcgatg ggctgcgccg gatcgtggcg
    12001 aacacggtgg agaagcgcct gcgcaacgcg cgcggcttgc cgaacttcgg gatcagcttc
    12061 tacgtgctgg acaagcaggg ccggcatgcc ggggtgtcga tgtacgccgg cggcaagtac
    12121 gcggtctgcg acgacaacgg cggccgccag gtcgtgatgg acgcgctact ggacggcaag
    12181 ccaggcgcgt gatcgctgga gcgcacgcgt tacccgcgtg cgacaatgcc cgcggcggag
    12241 tcggccaggc agtcgcgtca tcacgtcgca aggcggggtg ccgaggaaag tccgggctcc
    12301 acagggcacg gtgccaggta acgcctgggc ggcgcgagtc gacggaaagt gcaacagaaa
    12361 gataccgcct atgtcggagc aatctggcag gcaagggtga aatggtgcgg taagagcgca
    12421 ccgcgagcct ggcaacaggc cggcatggca aaccccaccg ggagcaaggc caaataggga
    12481 cctcatgacg ctgcccgcgt cgggtccggg taggctgctt gagcgtacgg gtgaccgtgc
    12541 gcctagagga atgactgtcg cggcgcgcga gcgtcgaaca ggacccggct tatcggccga
    12601 ctccgccacc ttccatgctg cgcatggatg caagcctgcg cctcaggcgt aattgcggag
    12661 cacgctttcg ccctcgaaca cgccgtgtgg cccgaaccgg cgttcgtgga tgaagccgcg
    12721 gccgtcgcgg gcgacggcga tcacggtgct ggcccgcgtt ccgtaggttt cgccacggat
    12781 gaacgtgggg gacagccggc gctccaggtc gatgccaacc ccggtggccg gcaggtccgc
    12841 gtcggccgcg acatgctcgt cggcaagcgc gtcccagacc gcctgcaggt cgtcgctgcc
    12901 aagatcgatc cacccctgca gtgcggcgac caggcgacgg gtcttcggcc agggtgcgtc
    12961 caggcggccg ttggagatgc cgtgcacgcc ggccgccagc acgtcccgct ggcgcggatg
    13021 gttgcccagg tgccagcaac ctcccgcatc ggccagcatc aggttgaacg gcgcgtaggc
    13081 gtgggcgccg gccagcaagg cgtcggtggt gccggcggca tccgccgatc cggccaggaa
    13141 ggcgtgcggc agtgccccgc gcgacgggcc gtcgtgcggc ttggcgaagc cgtcgcggac
    13201 attggtgacc accgcgaccc ggccgcgggt gtcgagcccg acccaggtgc cgccggattg
    13261 caggtcgcgc ccggcgagca ggccgctgtc cggccagcgg gccacggccg cggtcgggcg
    13321 ggcgtggaat tcgtcccggt tgccggccag cagcagccgc cagcgggggt gggcattgca
    13381 ggcgaaggcg acgacgcaca tggggcaatt gtggcgcgtc cgggggcgcc cgcgtgcatt
    13441 catgttttgc accgcaacat gccccggcac gcgggccggg ccaccggcgc cgaagtgtgt
    13501 aggaagtcac gaattctcaa aaaatgagac gaaacatggg cttgtggata aagcttgaac
    13561 aagtctctca agattgctgc aagtcgttga cctgcctagt gaaattcccg gaggaatgtt
    13621 gttgacaagg caaaaacgcg tgcctaaggt ggcgtcttgt ggggaaaccg ggaatttcgt
    13681 ggtttttccg tcatgaaccc cgcttgagac gggggcgaca cagggcaggg gcatgttcca
    13741 gggcgaaacc gccatcagca tcgacgacaa gggccgcctg gcgattccca ccgcctaccg
    13801 ggaccttgtc gcgcgcgaat gcggcaacca gctggtgctg acctacagcc cgttcgagga
    13861 gggctgcctc tacctgtacc cgctgccggt ctgggaaaag gttcgcgacc aggtcaatgc
    13921 cctgccgcgg accaagaaga acagccgcct gctgcagctc aagctggtcg gcgcggcggc
    13981 attcgccgag ccggatggca gcggccggat ctcgatcccg gccagccagc gcagcgcggt
    14041 ggggatcgac cgcaaggccg tgctgctggg catgggcgac aagttcgagt tgtggagcga
    14101 gcaggcgcac cacgcgcaga tccggcagac gctgggtgat gccgacatgg gcgacgacct
    14161 gcaggacctg gtgttgtgag caccggtggc gcgggcgcca cgcctgccca ccagccagtg
    14221 cgtcacttgc cggtgatgtt ctccgaggtg atggagggct tggcggtgaa ggcgggtgga
    14281 acctacctgg atggcacctt cggccgcggc ggccacgcac gcggggtgct ggcgcagctg
    14341 ggccccgccg gccgcctgct ggtgatggac aaggatcccg aggcgatcgc cgaggccggg
    14401 cgactggcgc atgacgacgc acgcgtggcc tggcgccgcg gcagcttcgc cgacctcgcc
    14461 cgctggccgc tggccgatcc gggcctcgac ggggtgctgc tggacctcgg cgtgtcgtcg
    14521 ccgcagctgg acgtcgccga gcgcgggttc tcgttcggca aggacggccc gctggacatg
    14581 cggatggatc cggacagcgg cgaaagcgcc gcgcagtggc tggcgcatgc gtccgaaaag
    14641 gacatcgccg acgtgctgtg gacctatggc gaggagcgcc agagccgtcg catcgcgcgg
    14701 gccatcgttg cccgccgcgg cgacgcgccg ttgctgcgca ccgcgcagct ggccgagctg
    14761 atcgcctcgg tgatgccgcg cggcgacagc aagatccatc ccgcgacccg cagcttccag
    14821 gccatccgga tcttcatcaa ccgcgaactg gtggacctcg aggccgggct cgacgccgcg
    14881 ctcgagcggc tcaagccggg tggccggctg gcggtgatca gcttccattc gctggaagac
    14941 cgcatcgtca agcggttcat ggccagccac gcgaaggcgc cgccggcgaa ccggcggatg
    15001 ccggtggaac tcccgttcac cccggtcctg cggctgctcg atggtgccca gaaggcgggt
    15061 ccggcggaac tggccgccaa tccgcgcgcg cgcagcgcag tgctgcgtgt cgccgagaag
    15121 ctggcggccg ccggcggggc ggtggcggca tgaaccgcta cctggtcctc gccgtgctgg
    15181 tggccgcgaa catggcgacc gcgctgctgg tggtgcgcga ccgccacgag caccggctgg
    15241 cgttcgtcgc cctcagcaag ctggagaagg cgcgcgacga cctcaacatc gagttcggcc
    15301 gcctgcagct ggaacaggcg acctgggcgg aaagcaaccg cgtcgaccag gtcgcgcgga
    15361 gccggctggg catggtgttc ccgcgcaccg aggacatcgt ggtgctgcgg cgatgaaatt
    15421 gcccggcttg cggctcccga acctgcaaaa gcgcgacccg gcgcgtcgcg acggcgtgcg
    15481 cgcgcccgcc ggcaaccgtc cgcgcggccg cacccagttc aacctgcgcg ggcggctgct
    15541 gctggtggtc ggaacgctgg cgctgtgctc ggtggcgctg ctggcgcgtg cgctggacct
    15601 gcaggtggtc gacaaggcgt tctaccagca gcagggcgcg gcgcgcttcc tgcgcgagat
    15661 cccgatcgcc acc
//
LOCUS       DAORUK010000036        15575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_3552_length_15575_cov_22.170941, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000036 DAORUK010000000
VERSION     DAORUK010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15575
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3552_length_15575_cov_22.170941"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PK818_03940"
     CDS             complement(<1..1012)
                     /locus_tag="PK818_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQY81918.1"
                     /translation="MSAETTHDIKFTDLGLPETLLQALRTVGYESPSPIQAATIPPLL
                     AGRDMIGQAQTGTGKTAAFALPALARLDATAGKPQVLVLAPTRELAIQVAEAFQKYAN
                     HIPGFHVLPIYGGQSYQPQLSALKRGVHVVVGTPGRVIDHLERGSLDLSALRMLVLDE
                     ADEMLRMGFIDDVEAVLKKTPETRQVALFSATMPAQIKRIAQTYLKDPVEIAIKSLTS
                     TAENIRQRFWMVSGINKLDALTRIMEAEPFEAMIVFARTKLGTDELAEKLAARGFSAA
                     AINGDVDQKMRERTIQRLKDGQIDILVATDVAARGLDVDRISHVLNYDIPYDTESYVH
                     RIG"
     gene            1124..2683
                     /locus_tag="PK818_03945"
     CDS             1124..2683
                     /locus_tag="PK818_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HQY81919.1"
                     /translation="MHLPSFDNRFLRSLPGDPEPRNFIRQVRAALWSAVAPAPVAAPR
                     LLAHSQEMAAELGFSEADVSSPEFARVFAGNALLPGMQPFASNYGGHQFGHWAGQLGD
                     GRAISLGEAVLADGQRRELQLKGAGPTPYSRSADGRAVLRSSIREFLCSEAMHHLGIP
                     TTRALSLVGTGEGVVRDMFYDGHPEVEPGAIVCRVAPSFLRFGHFELPSQRGDTSLLQ
                     ALADFCIARDFPHLRGRGEPLYADWFAEVCERTARLVAGWMRVGFVHGVLNTDNMSIL
                     GLAIDYGPYGWIDAYDPDWTPNTTDATGRRYRFGWQPRVANWNLGQLARALAPLFTDA
                     APLQAGLDRFGAAFAAADRDDTARKLGLAECGDADVALLRDLHGLLHAHEVDMTLWFR
                     ALADIDPRAPSLAPLEHAFYDAGKRTAGEAGLNDWLARYAARVREDALSVDGRRARMH
                     AANPKFVLRNWLAQEAIDRAHAGDMAGIGELLAVMRRPYEEQPGNERFAGKRPDWAKD
                     RAGCSMLSCSS"
     gene            2885..4771
                     /locus_tag="PK818_03950"
     CDS             2885..4771
                     /locus_tag="PK818_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010372732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY81920.1"
                     /translation="MPLITLQSVDFSVGGPLLLENVDLAIDPGERIALIGRNGAGKST
                     LLKLLSGDLRPDDGEVRVEGGRRIARLEQEVPQDAGGSVFDVVAGGLGELGTLLADYH
                     HLIHAEHIDTDALAAVQTRIEAAHGWALDRRVLETLERLQLDGDADFGALSGGMKRRV
                     LLARALVSQPDLLLLDEPTNHLDIAAIDWLEGFLKQWSGALVFITHDRRFLRALATRI
                     VEIDRGQVTSWPGDWDNYLRRKEERLNAEAQENARFDKLLAQEEVWIRQGIKARRTRD
                     EGRVRRLQAMRSERAGRRGQAGNVRMATAQAGESGKKVIEARGVSFGHGDGDLVHEFS
                     TTILRGDRIGLVGPNGSGKTTLLKLLLGELQPRSGEMKRGTQLQVAYFDQYRATLRED
                     WNAIENVAEGRESVTVGGRPKHVIGYLQDFLFTPERARAPITRLSGGERNRLLLAKLF
                     AQPSNLLVMDEPTNDLDVETLELLEELLADYPGTLLLVSHDRDFLDNVVTSTLAMEGG
                     GRVGEYVGGYSDWLRQRPSPAATVTAAAPKPQAAQATPSAGAPTPPAKAKLSYKDARE
                     LEQLPARIDALEAQVAAFTARMAEAGYYQRDVAAITADNAALAAAQAELDAAYSRWGE
                     LEAG"
     gene            4914..5270
                     /locus_tag="PK818_03955"
     CDS             4914..5270
                     /locus_tag="PK818_03955"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HQY81921.1"
                     /translation="MLAEYLPTLLFLIVATGIGIALIIVGNVLGPKRPTAEKLSPYEC
                     GFGAFENARAQFDVRYYLVAILFIVFDLEIAFVFPWALVFRELGVFGLIEMGVFLGLL
                     VIGFIYVWKKGALEWE"
     gene            5261..5875
                     /locus_tag="PK818_03960"
     CDS             5261..5875
                     /locus_tag="PK818_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HQY81922.1"
                     /translation="MGVSETISGLMHNPLPEGRLDDILRPEGDNPLLQNGFVTTSMDV
                     LWNWARTGSMWPMTFGLACCAVEMMHAGAARLDLDRYGVVFRPSPRQSDVMIVAGTLC
                     NKMAPALRKVYDQMPDPKWVISMGSCANGGGYYHYSYSVVRGCDRVVPVDVYVPGCPP
                     TAEALVYGILQLQKKIRRSAQNPFAPGGGGNEAGHATMNKGIVA"
     gene            5872..6633
                     /locus_tag="PK818_03965"
     CDS             5872..6633
                     /locus_tag="PK818_03965"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HQY81923.1"
                     /translation="MSASLANELAARFGAAAVEVALPRGETTLEVPAGEWVAACTALR
                     DAFGFDTFIDLCGVDYLGYGNDEWDTDGVSAQGFSRGVEGRGPGRFKWGERPSHQVGE
                     PAAVAADTLEGKRFAVVLHLLSVRRNLRLRVRCFAADNSLPVVASLVGVWPGVNWFER
                     EAFDLFGIIFEGHPDLRRILTDYGFVGHPFRKDFPLIGNVEVRYDAGLKRVVYEPVTS
                     VEPRVGVPRVIRDDARFATAEGERGGKILDREARR"
     gene            6630..7934
                     /locus_tag="PK818_03970"
     CDS             6630..7934
                     /locus_tag="PK818_03970"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005417935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HQY81924.1"
                     /translation="MSAVPTSGLFASNAGEALQEIRNYTLNFGPQHPAAHGVLRLILE
                     MDGEVIRRADPHVGLLHRGTEKLAESKPFNQSIGYMDRLDYVSMMCNEHAYVLAIERL
                     MGIEAPERAQWIRTMFDEITRILNHLMWVGSNALDLGAMAVFLYAFREREELMDCYEA
                     VSGARMHATYYRPGGVYRDLPAKMPRYKESPWRKGAKLKRFNQWREGSLLDYLEHFCK
                     DFPARVDEYETLLTDNRIWKQRTVGIGVIPPEQAMAWGMSGPMLRGSGVAWDLRKKQP
                     YAKYAEVDFDIPVGVKGDCYDRYLVRMAEMRQSTRIIEQCVRWLKANPGPVMLDNYKV
                     SPPSREAMKDDMEALIHHFKLFSEGYCVPAGETYAAVEAPKGEFGCYLVSDGANKPFR
                     VHLRAPGFAHLSSMDEIVKGHMLADVVAMIGTYDIVFGEVDR"
     gene            7931..8458
                     /gene="nuoE"
                     /locus_tag="PK818_03975"
     CDS             7931..8458
                     /gene="nuoE"
                     /locus_tag="PK818_03975"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HQY81925.1"
                     /translation="MKATGNFENARSVDPMVALSDATRAHIDHWLAKFPADRKRSAVL
                     QGLFAAQEQNGGWLSDEMIAAVARYLDLPPVWAYEVATFYSMFETKPVGRNNVAFCTN
                     ISCWLNGAEDLVAHAEQKLGCKLGGSTADGRIYLKREEECVAACCGAPVVVINGHYHE
                     KLDAAKVDELLDGLK"
     gene            8458..9828
                     /gene="nuoF"
                     /locus_tag="PK818_03980"
     CDS             8458..9828
                     /gene="nuoF"
                     /locus_tag="PK818_03980"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HQY81926.1"
                     /translation="MGGHHSHYSEGYGPVGPAPTEHSVVYTTLHFDKPWSYENYLKTG
                     GYSALRRILEERIPPADVVEMVKQSGLRGRGGAGFPTGLKWSFMPKGPGQKYILCNSD
                     ESEPGTAKDRDILRYNPHAVIEGMAIACYATGATVAYNYLRGEFHHEPFEHLEEATRE
                     AYANGWLGKDVLGSGIDIDIHNALGAGAYICGEETALMESLEGKKGQPRYKPPFPANF
                     GLYGKPTTINNTETYASVPAIVRNGAEWFAGLGKPNNGGPKVFSVSGHVARPGNHEIR
                     LGTPFADLLQLCGGIRAGHRIKAVIPGGSSMPVLPGEVMMGLTMDYDSIQKAGSGLGS
                     GAVIVMDETTCMVRACQRISRFYYAESCGQCTPCREGTGWMYRMLTRIANLEATMDDL
                     HMLRASAGQIEGHTICAFGEAAAWPVQGFLRHFWHEFEYAIVNKRFYVDDERAGTLAK
                     TAQVAA"
     gene            9825..12047
                     /gene="nuoG"
                     /locus_tag="PK818_03985"
     CDS             9825..12047
                     /gene="nuoG"
                     /locus_tag="PK818_03985"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012511707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HQY81927.1"
                     /translation="MSAQPVNPNLPPDHVTVFIDGTELAAPRGSMIIHAADKAGIPIP
                     RFCYHDKLPIAANCRMCLVEVEKMPKPAPACATPVMDGMKISTRSDKALKSQRNVMEF
                     LLINHPLDCPVCDQGGECELQDLSLGYGRSVSRFVERKRAVADEDLGPLVATEMTRCI
                     HCTRCVRFTAEIAGTYELGGMQRGENLQIGTYDGKPLTTELSGNVIDVCPVGALTNKV
                     FRFKARPWELTAKASLGYHDALGSNLFHHVRRGEILRSVPRDNEAVNECWLSDRDRYA
                     HEGLYAEDRATVPLVRDGDGFREASWDEALAAAARILRDNAGDNLGVLVHPATSNEEG
                     ALLARLAGALGTGNLDHRIGQLDLSDGAQAQAFAMPVAEIEQADAIVILGSNLRHEVP
                     LLHQRVRKAAKRGAKIHVVNPVDFDFAFTVASSRIVPPAQLAQALAQVDLGDASNVAL
                     IVGGVAENGAHAAAIRAAANEFANAKNARLCRIPQGANALGLSRHGVLPAARDAQSML
                     RDPRAAYLVYGLEPGLDFADQHAALKALGSARVVAFSQFACQSTRRVADVILPIGALP
                     EIEATLGNLDGIAQATSAAGKLPGQARPGWRVLRALATELALPGFEFTDLAGLRAGIA
                     PVAMQAASGQPVATGGGGLEVAVSQAIYRGDGVLRRSQALQAHPLTVGARVALNPADA
                     AAAGLAAGAMAKLRNSVGAATLQVVIDDRVAPGCAWIESGYGATAPLAASATVEVVGA
                     "
     gene            12065..13135
                     /gene="nuoH"
                     /locus_tag="PK818_03990"
     CDS             12065..13135
                     /gene="nuoH"
                     /locus_tag="PK818_03990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009590711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HQY81928.1"
                     /translation="MLVEPLRDGLLSFGTIGMLVWIVLKILAIAMPVIVTVAFYVVWE
                     RKLIGWMHVRHGPMYIGKGILQAFADVFKLLFKEVVQPTVAHPFLFRLAPLIALVPAF
                     AAWAVVPFDAQLVLSNANAGLLYLLAMTSMGVYGIILAGWASNSKYAFLGAMRAAAQV
                     VSYEIAMGFALVGVLIAAGSLNLSEIVMAQSGDAGFLEWFWVPLLPLFVVYFISGVAE
                     TNRAPFDVVEGESEIVAGHMVEYSGSQFALFFLAEYANMILVSFLTALFFLGGWLSPI
                     PASWIPDVPLLGTVLGDGWWWLLAKVFFFASCFIWFRASFPRYRYDQIMRLGWKVFIP
                     ITIAWICATALMAYFNVFTAGQ"
     gene            13142..13630
                     /gene="nuoI"
                     /locus_tag="PK818_03995"
     CDS             13142..13630
                     /gene="nuoI"
                     /locus_tag="PK818_03995"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HQY81929.1"
                     /translation="MTRIASYLKSLLLLELAGGLWLTFKYLFRPKYTLMYPAEKTPQS
                     PRFRGVHALRRYPNGEERCIACKLCEAVCPALAITIDSEQRADDTRRTTRYEIDLFKC
                     IYCGFCEESCPVDSIVETHVHEYHFDKRGQNIVTKPQLLAIGDRLEAEIAERRAADAP
                     FR"
     gene            13644..14291
                     /locus_tag="PK818_04000"
     CDS             13644..14291
                     /locus_tag="PK818_04000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HQY81930.1"
                     /translation="MTPDFPTLAFLGFAATAAIAALATITARNPVHAALWLVLTFFSV
                     ACTWIIGGAEFLGVALVLVYVGAVMVLFLFVVMMLDVDMAPLREGYVRYLPVGLVVAL
                     VMLAQILALIGVKAAAEVPFADNAAVAADVSNTTWLARALFTEFMLPFEVAAVILTVA
                     VVAAVMLTLRRRGGTKHQDPSAQSRVRASDRLRMVKMDAERPALPAAAPESEAAP"
     gene            14297..14620
                     /gene="nuoK"
                     /locus_tag="PK818_04005"
     CDS             14297..14620
                     /gene="nuoK"
                     /locus_tag="PK818_04005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006450557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HQY81931.1"
                     /translation="MPNIFAALTLGHYLALGAVLFCISVAGIFLNRKNVIMLLMSLEL
                     MLLAVNINFVAFSRSLGDAAGQVFVFFILTVAAAESAIGLAILVTLFRNRRTIDVADI
                     DTLKG"
     gene            14624..>15575
                     /locus_tag="PK818_04010"
     CDS             14624..>15575
                     /locus_tag="PK818_04010"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HQY81932.1"
                     /translation="MVISKTHLLLVVLLPLLGAVLAGLFGRKIGRVGAHSATILGVAA
                     SCVLSCWVLWQLLQGAAPFNENVYTWFQVGGIQANVGFMVDKLTAMMMVVVTFVSLLV
                     HIYTIGYMAEDDGYQRFFSYISLFTFSMLMLVMSNNFLQLFFGWEAVGLVSYLLIGFW
                     FKKPSAIFANLKAFLVNRVGDFGFLLGIAGVLYWIGTLDYATAFANAPTVRDATVSLW
                     AGSEWSVPTVICICLFIGAMGKSAQVPLHVWLPDSMEGPTPISALIHAATMVTAGIFM
                     VARMSPLFEMSPAALNFILFIGATTAFWTGLIGMVQNDIKR"
BASE COUNT         2381 a         5226 c         5395 g         2573 t
ORIGIN      
        1 ggccgatgcg gtgcacgtag ctctcggtgt cgtacgggat gtcgtagttc agcacgtggc
       61 tgatgcggtc cacgtccagc ccgcgcgcgg ccacgtcggt ggccaccagg atgtcgatct
      121 ggccgtcctt gaggcgctgg atggtgcgct cgcgcatctt ctggtcgacg tcgccgttga
      181 tcgccgccgc ggaaaagccc ctcgcggcaa gcttctcggc cagctcgtcg gtgcccagct
      241 tggtgcgcgc gaacacgatc atcgcctcga acggctcggc ctccatgatc cgggtcagcg
      301 cgtccagctt gttgatgccg ctgaccatcc agaagcgctg gcggatgttc tcggcggtgg
      361 aggtcagcga cttgatcgcg atctcgaccg gatccttcag gtaggtctgg gcgatgcgct
      421 tgatctgggc cggcatggtg gccgagaaca gcgcgacctg gcgcgtctcc ggggtcttct
      481 tgaggaccgc ctcgacgtcg tcgatgaagc ccatccgcag catttcgtcg gcctcgtcca
      541 gcaccagcat gcgcagcgcg gacaggtcca gcgagccgcg ctccaggtgg tcgatgaccc
      601 ggccgggggt gccgaccacg acgtgcacgc cgcgcttcag ggcgctgagc tgcggctggt
      661 agctctggcc gccgtagatc ggcagcacat ggaacccggg gatgtggttg gcgtacttct
      721 ggaacgcctc ggcgacctgg atcgccagtt cgcgggtcgg tgccagcacc agcacctgcg
      781 gcttgccggc ggtcgcgtcc agccgcgcca gcgcgggcag ggcgaatgcc gcggtcttgc
      841 cggtgccggt ctgggcctgg ccgatcatgt cgcggccggc cagcagtggc gggatcgtcg
      901 ccgcctggat cgggcttggc gattcgtagc cgaccgtgcg cagggcctgc agcagggtct
      961 cgggcaggcc gaggtcggtg aacttgatgt cgtgggtggt ttcggcgctc atatgcgtcc
     1021 catgtgaatg attcagtggg cgtagccgcc gcgggtttga ccgcacagtg tacgcccgcc
     1081 tgcctgagcg aaggctccaa cggcgggcgg gctacgatgc ggcatgcacc tgccgagctt
     1141 cgacaaccgc ttcctgcgct cgcttcccgg ggacccggag ccgcgcaact tcatccgcca
     1201 ggtgcgcgcc gcgctgtggt cggcggtcgc tccggcgccg gtcgccgcgc cgcggctgct
     1261 ggcgcattcg caagaaatgg ccgccgaact gggcttcagc gaggccgacg tcagctcccc
     1321 ggagttcgcc cgggtgttcg ccgggaatgc gctgttgccg gggatgcagc cgttcgcgag
     1381 caactacggc ggccaccagt tcgggcattg ggcgggccag ctcggcgacg ggcgcgcgat
     1441 ttcgctgggc gaagcggtgc tcgccgatgg ccagcgccgc gagctgcagc tgaagggtgc
     1501 gggtccgacc ccctattcgc gctccgccga cggtcgtgcg gtgctgcgct cctcgatccg
     1561 cgaattcctg tgcagcgagg cgatgcacca cctgggtatc ccgaccaccc gtgcgctgag
     1621 cctggtcggc accggcgagg gcgtggtccg cgacatgttc tacgacggcc atcccgaagt
     1681 ggagccgggc gccatcgtct gccgggtggc gccgtccttc ctgcgcttcg gccatttcga
     1741 gctgccgtcg cagcgcggcg acaccagcct gctgcaggcg cttgccgact tctgcatcgc
     1801 acgcgatttc ccgcacctgc gggggcgggg cgagccgctt tacgccgact ggttcgcgga
     1861 agtctgcgag cgtaccgctc ggctggtggc cgggtggatg cgggtcggct tcgtgcacgg
     1921 ggtgctcaac accgacaaca tgtcgatcct cggcctggcc atcgactacg gtccgtacgg
     1981 ctggatcgac gcctacgatc ccgactggac cccgaacacc accgacgcca cgggccggcg
     2041 ctaccggttc ggctggcaac ccagggtggc gaactggaac ctgggccagc tggcgcgtgc
     2101 gctggcgccg ctgttcacgg atgccgcgcc gctccaggcc ggcctggacc gctttggcgc
     2161 ggccttcgcc gccgctgacc gcgacgacac cgcgcgcaag ctggggctgg cggaatgcgg
     2221 cgatgccgat gtcgcgctgc tccgcgacct gcacggcctg ctgcacgcac acgaggtcga
     2281 catgaccctg tggttccggg ccctggccga tatcgatccg cgcgcgccct cgctggcacc
     2341 gctggagcat gcgttctacg acgccggcaa gcgcaccgcg ggcgaggctg ggttgaacga
     2401 ctggctggcg cgctacgccg cgcgcgtgcg cgaggacgcg ctgtccgtcg atggcaggcg
     2461 tgcgcggatg cacgccgcca acccgaagtt cgtgttgcgc aactggctgg cgcaggaggc
     2521 catcgatcgc gcgcatgccg gggacatggc cggcatcggc gaattgctgg cggtcatgcg
     2581 ccggccatac gaggaacagc cgggcaatga acgattcgcc ggcaagcgcc ccgactgggc
     2641 gaaggaccgg gccggctgtt cgatgctgtc ctgcagctcc tgacaaccgg tccggtggcc
     2701 gggcttactt gcccttcttt tccttcttcg ccgcgcgctt ttccttcagc gacttctccg
     2761 gcttcttctt ctcgctcttc ttctggtcca tgcccttgct catcgcactg tccttttcga
     2821 ggtggattgg gtggcacgcg ccccggccgc ccggttccgc agggggcgcg gactacaatg
     2881 gtgcatgccc ctgatcacct tgcaaagcgt cgacttcagc gtcggcggcc cgctgctgct
     2941 ggaaaacgtc gaccttgcca tcgatcccgg cgagcgcatc gccctgatcg gccgcaacgg
     3001 cgccggcaag tcgaccctgc tgaaactgct gtccggcgac ctcaggcccg atgacggcga
     3061 ggtgcgggtc gaaggcggcc gccggatcgc gcggctggag caggaagtac cgcaggacgc
     3121 gggcggcagc gtgttcgacg tggtcgccgg cgggctgggc gagctcggca ccctgctggc
     3181 cgactaccac cacctgatcc acgccgagca catcgatacc gacgccctgg ccgcggtgca
     3241 gacccggatc gaggccgcgc atggctgggc gctggaccgg cgggtgctgg aaacgctgga
     3301 acgcctgcaa ctggacggcg atgccgactt cggggcgctg tccggcggca tgaagcgtcg
     3361 ggtgctgctg gcgcgggcac tggtgtcgca gccggacctg ctgctgctgg acgagccgac
     3421 caaccacctc gacatcgccg cgatcgactg gctggaaggt ttcctcaagc agtggagcgg
     3481 ggcgctggtg ttcatcaccc acgaccgccg cttcctgcgc gcgctggcca cccgcatcgt
     3541 cgagatcgac cgcggccagg tgaccagctg gccgggcgac tgggacaact acctgcgccg
     3601 caaggaggag cggctgaacg ccgaggcgca ggagaacgcg cgcttcgaca agctgctggc
     3661 gcaggaggaa gtgtggatcc ggcagggcat caaggctcgc cgcacccgcg acgagggccg
     3721 cgtgcgccgg ctgcaggcga tgcgcagcga acgcgccggg cgccgcgggc aggccggcaa
     3781 cgtacggatg gccaccgccc aggccggcga atccggcaag aaggtgatcg aggcgcgcgg
     3841 ggtcagcttc ggccatggcg atggcgacct ggtccacgag ttctccacca cgatcctgcg
     3901 cggcgaccgc atcggcctgg tcgggcccaa cggcagcggc aagaccacct tgctgaagct
     3961 gttgctgggc gagctgcagc cgcggtccgg cgagatgaag cgcggcacgc agttgcaggt
     4021 cgcctatttc gaccagtacc gcgccaccct gcgcgaggac tggaacgcga tcgagaacgt
     4081 ggccgagggc cgcgagagcg tgaccgtggg cggcaggccg aagcacgtga tcggctacct
     4141 gcaggacttc ctgttcaccc ccgagcgcgc ccgcgcgccg atcacccggc tgtccggcgg
     4201 cgagcgcaac cgcctgctgc tggcgaagct gttcgcgcag ccgtccaacc tgctggtgat
     4261 ggacgaaccc accaacgacc tcgacgtcga gaccctggaa ctgctggagg aactgctggc
     4321 ggactacccg gggaccctgc tgctggtcag ccacgaccgc gatttcctcg acaacgtggt
     4381 gacctcgacg ctggcgatgg aaggcggcgg ccgggtcggc gaatacgtgg gtggctacag
     4441 cgactggctg cggcagcggc cgtcgcctgc cgcgaccgtc accgcggccg cgccgaagcc
     4501 gcaggccgcg caggcaaccc cgtccgcagg tgcacccacg ccaccggcga aggcgaagct
     4561 gagctacaag gacgcgcgcg aactggaaca gttgccggcg cgcatcgacg cgctggaggc
     4621 gcaggtcgcg gcgttcaccg cgcggatggc ggaagccggc tactaccagc gcgacgtggc
     4681 ggcgatcacc gcggacaatg cggcgctggc ggcggcccag gccgagctgg acgcggccta
     4741 ttcgcgctgg ggcgaactcg aggcgggctg acgcgatccg ggcccgtgcg ggcggccaac
     4801 ccacgccggt gattgcatcc gggtctggcc gatacagttc cggggcggct acaatggcgg
     4861 ggataagcgg gcgcaactgc agcctgcttg cccatgacta gtccgaggcg cgcgtgctgg
     4921 ccgaatacct gccaaccctg ctgttcctga tcgttgccac cggcatcggc atcgccctga
     4981 tcatcgtcgg caacgtgctg ggcccgaagc gcccgaccgc cgagaagctg tcgccgtacg
     5041 aatgcggctt cggcgcgttc gagaacgcac gcgcgcagtt cgacgtgcgc tactacctgg
     5101 tcgcgatcct gttcatcgtg ttcgacctcg aaatcgcctt cgtgttcccg tgggcgctgg
     5161 tgttccgcga actgggcgtg ttcggcctga tcgagatggg cgtgttcctg ggcctgctgg
     5221 tgatcggctt catctacgta tggaagaagg gcgccctcga atgggagtga gcgaaaccat
     5281 ctccggcctg atgcacaacc cgctgcccga agggcggctg gacgacatcc tgcgcccgga
     5341 aggcgacaac ccgctgctgc agaacggctt cgtcaccacc agcatggacg tgctgtggaa
     5401 ctgggcgcgc accggctcga tgtggccgat gaccttcggc ctggcctgct gcgcggtgga
     5461 gatgatgcac gccggcgccg cgcgcctgga cctggaccgc tacggcgtgg tgttccgccc
     5521 gtcgccacgg cagtccgacg tgatgatcgt ggccggcacc ctgtgcaaca agatggcccc
     5581 ggccttgcgc aaggtctacg accagatgcc cgaccccaag tgggtgatct cgatgggcag
     5641 ctgcgccaac ggcggcggct actaccacta ttcctattcc gtggtccgcg gctgcgaccg
     5701 ggtggtgccg gtcgacgtgt acgtgcccgg ttgcccgccc accgcggaag cgctggtgta
     5761 cggcatcctg cagttgcaga agaagatccg ccgcagcgcg cagaacccgt tcgcgccggg
     5821 cggcggcggc aacgaagccg gccacgccac catgaacaag ggcatcgtgg catgagcgcg
     5881 tcgctggcga acgaacttgc ggcgcgcttc ggcgcggccg cggtcgaggt cgcgctgccg
     5941 cgcggcgaga ccacgctcga ggtccccgcg ggcgaatggg tggcggcctg caccgcgctg
     6001 cgcgatgcat tcggcttcga taccttcatc gacctgtgcg gggtcgacta cctgggctac
     6061 ggcaacgacg agtgggatac cgacggcgtt tccgcccagg gattcagccg cggggtcgag
     6121 ggccggggac cgggtcgctt caagtggggc gagcggccga gccaccaggt cggcgaaccc
     6181 gccgcggtgg ccgcggacac gctcgaaggc aagcgcttcg ccgttgtcct gcacctgttg
     6241 tcggtgcgtc gcaacctgcg cctgcgggtg cgttgcttcg ccgccgacaa cagcctgccg
     6301 gtggtggctt cgctggtcgg ggtgtggccc ggcgtcaact ggttcgagcg cgaggcattc
     6361 gacctgttcg ggatcatctt cgaggggcat ccggacctgc gccggatcct caccgactac
     6421 ggcttcgtcg gccatccgtt ccgcaaggac ttcccgctga tcggcaacgt cgaggtccgc
     6481 tatgacgcag gcctcaagcg cgtggtctac gagccggtga cctcggtcga gccgcgcgtg
     6541 ggcgtgccgc gggtgatccg cgacgacgcc cgcttcgcca ccgccgaagg cgagcgcggc
     6601 ggcaagatcc tcgaccggga ggcccgtcga tgagcgccgt gccgacgtcg ggcctgttcg
     6661 ccagcaatgc cggcgaagcc ctgcaggaga tccgcaacta caccctcaac ttcggcccgc
     6721 agcacccggc cgcgcatggc gtgctgcgcc tgatcctgga gatggacggc gaggtgatcc
     6781 gccgcgcgga tccgcacgtg ggcctgctgc accggggcac cgagaagctg gccgagtcca
     6841 agccgttcaa ccagtcgatc ggctacatgg accgcctgga ctacgtgtcg atgatgtgca
     6901 acgagcacgc ctacgtgctc gcgatcgaac ggctgatggg gatcgaggcg ccggagcggg
     6961 cgcagtggat ccgcaccatg ttcgacgaga tcacccggat cctgaaccac ctgatgtggg
     7021 tgggttcgaa cgcgctcgac ctcggcgcga tggcggtgtt cctgtacgcc ttccgcgagc
     7081 gcgaggagct gatggactgc tacgaggcgg tcagcggcgc gcgcatgcac gcgacctact
     7141 accgcccggg cggcgtctac cgcgacctgc ccgcgaagat gccgcgttac aaggaatcgc
     7201 cgtggcgcaa gggcgccaag ctcaagcgct tcaaccagtg gcgcgaaggc tcgctgctcg
     7261 attacctcga gcacttctgc aaggacttcc cggcccgcgt cgacgagtac gagaccctgc
     7321 tcaccgacaa ccgcatctgg aagcagcgca ccgtcggcat cggcgtgatc ccgccggaac
     7381 aggcgatggc ctggggcatg agcggcccga tgctgcgcgg ctccggcgtg gcctgggacc
     7441 tgcgcaagaa gcagccctac gcgaagtacg cggaggtcga tttcgacatc ccggtcgggg
     7501 tcaagggcga ctgctacgac cgttacctgg ttcgcatggc cgaaatgcgc cagtccaccc
     7561 gcatcatcga gcagtgcgtg cgctggctga aggccaaccc gggcccggtg atgctggaca
     7621 actacaaggt gtcgccgccg tcgcgtgaag cgatgaagga cgacatggaa gccctcatcc
     7681 accacttcaa gctgttctcc gaaggctatt gcgtccccgc cggcgagacc tacgcggcgg
     7741 tcgaggcacc gaagggcgag ttcggctgct acctggtgag cgacggcgcc aacaagccgt
     7801 tccgcgtgca cctgcgcgcg cccggcttcg cccacctgtc gtcgatggac gagatcgtga
     7861 agggccacat gctggccgac gtggtggcga tgatcggcac ctacgacatc gtgtttggag
     7921 aagtcgaccg atgaaggcga cgggcaattt cgaaaacgcg cgcagcgtgg acccgatggt
     7981 ggcgctgtcg gatgcgacgc gtgcgcacat cgaccactgg ctggccaagt tccccgccga
     8041 ccgcaagcgt tccgcggtgc tgcagggcct gttcgcggcg caggaacaga acggcggctg
     8101 gctcagcgac gagatgatcg ccgcggtcgc ccggtacctg gacctgccgc cggtatgggc
     8161 ctacgaggtc gcgaccttct actcgatgtt cgagaccaag ccggtcggcc gcaacaacgt
     8221 ggccttctgc accaacatca gctgctggtt gaacggcgcc gaggacctgg tcgcgcacgc
     8281 cgagcagaag cttggctgca agctgggcgg ttccaccgcg gatggccgca tctacctcaa
     8341 gcgcgaggaa gaatgcgtgg ccgcgtgctg cggcgcgccg gtggtggtga tcaacgggca
     8401 ttaccacgag aagctcgacg ccgcgaaggt ggacgaactg ctggacgggc tgaagtaatg
     8461 ggcggacacc acagccacta ttccgagggc tacggcccgg ttggcccggc gccgacggag
     8521 cacagcgtgg tctacaccac cctgcatttc gacaagccgt ggtcgtacga gaactacctg
     8581 aagaccggcg gctacagcgc gctgcgcagg atcctggaag aaaggatccc gccggcggac
     8641 gtggtcgaga tggtcaagca gtccggcctg cgcggccgcg gcggcgcggg cttcccgacc
     8701 ggcctgaagt ggagcttcat gccgaagggc ccggggcaga agtacatcct gtgcaattcg
     8761 gacgagtcgg agccgggcac cgccaaggac cgcgacatcc tgcgttacaa cccgcacgcg
     8821 gtgatcgagg gcatggcgat cgcctgctac gccaccggcg ccaccgtggc ctacaactac
     8881 ctgcgcggcg agttccacca cgagcccttc gagcacctgg aagaggccac ccgcgaggcc
     8941 tacgccaacg gctggctggg caaggacgtg ctcggcagcg gcatcgacat cgacatccac
     9001 aacgcgctgg gcgccggtgc ctacatctgc ggcgaagaga ccgcgctgat ggagtcgctg
     9061 gaaggcaaga agggccagcc gcgctacaag ccgccgttcc cggccaactt cggcctgtac
     9121 ggcaagccga ccacgatcaa caacaccgag acctatgcct cggtgccggc gatcgtgcgc
     9181 aacggcgcgg aatggttcgc ggggctcggc aagcccaaca acggcggccc caaggtgttc
     9241 tcggtgtccg gccacgtggc gcgtcccggc aaccacgaga tccgcctcgg caccccgttc
     9301 gccgacctgc tgcagctgtg cggcgggatc cgcgccggcc accggatcaa ggcggtgatc
     9361 ccgggcggtt cgtcgatgcc ggtgctgccg ggcgaggtga tgatgggcct cacgatggac
     9421 tacgacagca tccagaaggc cgggtccggg ctgggttcgg gcgcggtgat cgtgatggac
     9481 gagaccacct gcatggtccg cgcctgccag cgcatctcgc ggttctacta cgcggaatcc
     9541 tgcggccagt gcaccccgtg ccgcgaaggc accggctgga tgtaccggat gctgacccgg
     9601 atcgccaacc tcgaggcgac catggacgac ctgcacatgc tgcgcgcctc cgccgggcag
     9661 atcgagggcc acaccatctg cgcgttcggc gaagccgcgg cgtggccggt gcagggcttc
     9721 ctgcgccatt tctggcatga attcgagtac gcgatcgtga acaagcgctt ctacgtcgac
     9781 gacgagcgcg cgggtaccct ggccaagacc gcgcaggtgg ccgcatgagt gcgcagccgg
     9841 tcaatccgaa cctgccgccg gaccacgtca ccgtcttcat cgacggcact gagctggctg
     9901 cgccaagggg atcgatgatc atccatgccg ccgacaaggc cggcatcccg atcccgcgct
     9961 tctgctacca cgacaagctg ccgatcgcgg ccaattgccg gatgtgcctg gtcgaggtcg
    10021 agaagatgcc gaagccggca ccggcctgcg ccacgccggt gatggacggc atgaagatca
    10081 gcacccgcag cgacaaggcg ttgaagtcgc agcgcaacgt gatggagttc ctgctgatca
    10141 accacccgct ggactgcccg gtctgcgacc agggcggcga atgcgaactg caggacctgt
    10201 cgctgggcta cgggcgctcg gtcagccgtt tcgtcgagcg caagcgcgcg gtggcggacg
    10261 aggacctcgg tccgctggtc gccaccgaga tgacccgctg catccattgc acccgttgcg
    10321 tgcgcttcac cgcggagatc gccggcacct acgagctcgg cggcatgcag cgcggcgaga
    10381 acctgcagat cggcacctat gacggcaagc cgctgaccac cgagctgtcc ggcaacgtga
    10441 tcgacgtgtg cccggtcggt gcgctgacca acaaggtgtt ccgcttcaag gcgcgcccgt
    10501 gggagctcac cgcgaaggcc tcgctgggct accacgacgc gctcggcagc aacctgttcc
    10561 accacgtgcg ccgcggcgag atcctgcgca gcgtgccgcg cgacaacgaa gcggtgaacg
    10621 agtgctggct gtcggaccgc gaccgctacg cgcatgaagg cctgtatgcc gaagatcgcg
    10681 cgacggtgcc gctggtccgc gatggcgacg gtttccgcga ggcatcctgg gacgaggcgc
    10741 tggccgccgc ggcacgcatc ctgcgcgaca acgccggcga caacctcggc gtgctggtgc
    10801 acccggccac ctcgaacgaa gagggcgccc tgctggcgcg cctcgccggg gcgcttggca
    10861 ccggcaacct cgaccaccgc atcggccagc tcgacctctc ggacggcgcc caggcgcagg
    10921 cgttcgcgat gccggtcgcc gagatcgagc aggccgatgc catcgtcatc ctcggcagca
    10981 acctgcgcca cgaagtgccg ttgctgcacc agcgcgtgcg caaggccgcc aagcgcgggg
    11041 cgaagatcca cgtcgtcaac ccggtcgatt tcgatttcgc gttcaccgtc gcgtccagcc
    11101 ggatcgtgcc gcccgcgcag cttgcgcagg cgctcgcgca ggtcgacctg ggcgatgcat
    11161 cgaacgtggc attgatcgtt ggcggggttg ccgagaacgg cgcgcacgcg gccgcgatcc
    11221 gcgccgccgc gaacgaattc gcgaatgcga agaacgccag gttgtgccgg atcccgcagg
    11281 gcgcgaacgc gcttggcctg tcccgccacg gcgtgctgcc agccgcgcgc gatgcgcagt
    11341 cgatgctgcg cgatccgcgc gctgcgtacc tggtctacgg actggagccg gggctggact
    11401 tcgccgacca gcatgctgcg ttgaaggcac tgggcagtgc cagggtcgtt gcattcagcc
    11461 agttcgcctg ccagagcacg cgccgggtgg ccgacgtgat cctgccgatc ggcgcgctgc
    11521 cggaaatcga ggcgacgctg ggcaacctcg atggcatcgc acaggccacc tccgcggccg
    11581 gaaagctgcc gggccaggca cgtcccggct ggcgcgtgct gcgtgcactt gccaccgaat
    11641 tggcgttgcc gggcttcgag ttcaccgacc ttgccgggct gcgcgccggg atcgccccgg
    11701 tggccatgca ggccgcctcg gggcagccgg tcgcgactgg cggcggtggc ctggaggtcg
    11761 ccgtttcgca ggcgatctat cgcggcgacg gggtgttgcg tcgctcgcag gcgttgcagg
    11821 cgcacccgct gacggtcggc gcgcgcgtcg ccctgaaccc ggccgatgcc gccgccgcgg
    11881 gcctcgccgc gggcgccatg gcgaagctgc gcaacagcgt cggtgccgcg accctgcagg
    11941 tcgtgatcga cgatcgcgtc gcgccgggct gcgcatggat cgaatccggc tacggcgcaa
    12001 ccgcgccgct ggccgcctcg gccacggtgg aggtcgtcgg cgcatgagca atccgttgat
    12061 gaccatgctg gtggagccgc tgcgcgacgg gctgctgtcg ttcggcacga tcggcatgct
    12121 ggtgtggatc gtgctgaaga tcctcgccat cgcgatgccg gtgatcgtga ccgtggcgtt
    12181 ctacgtggtc tgggagcgca agctgatcgg ctggatgcac gtgcgccacg ggccgatgta
    12241 catcggcaag ggcatcctcc aggccttcgc cgatgtcttc aagctgctgt tcaaggaagt
    12301 ggtgcagccg accgtggcgc atccgttcct gttccggctt gcgccgctga tcgcgctggt
    12361 tccggcgttc gcggcgtggg cagtggtgcc gttcgacgcg caactggtgc tgtcgaacgc
    12421 gaatgcgggc ctgctgtacc tgctggcgat gacctcgatg ggcgtctacg ggatcatcct
    12481 cgccggctgg gcgtccaact cgaagtacgc cttcctcggc gccatgcgtg ccgccgcgca
    12541 ggtggtcagc tacgagatcg cgatggggtt cgccctggtc ggggtgctga tcgccgccgg
    12601 cagcctgaac ctgagcgaga tcgtgatggc gcagtccggc gatgccggct tcctggagtg
    12661 gttctgggtg ccgctgctgc cgctgttcgt ggtctatttc atctccggcg tggccgagac
    12721 caaccgcgcg ccgttcgacg tggtcgaggg cgaatcggag atcgtcgccg ggcacatggt
    12781 ggagtactcg ggctcgcagt tcgcgctgtt cttccttgcc gaatacgcca acatgatcct
    12841 ggtcagcttc ctgaccgcgc tgttcttcct cggtggctgg ctgtcgccga tcccggcgtc
    12901 ctggatcccc gacgtgccgc tgctgggaac cgtgctgggc gacggctggt ggtggctgct
    12961 ggcgaaggtg ttcttcttcg cgagctgctt catctggttc cgcgccagct tcccgcgcta
    13021 tcgctacgac cagatcatgc ggctgggctg gaaggtgttc atcccgatca ccatcgcctg
    13081 gatatgcgcg accgcgctga tggcctactt caacgtgttc acggcggggc agtgacctga
    13141 aatgacgcgc atcgcctcgt acctcaagag cctcctgctg ctggaactcg ccggcggcct
    13201 ctggctgacc ttcaagtacc tgttccgtcc caagtacacg ctgatgtacc cggcggagaa
    13261 gaccccgcag tcgccgcgct tccgtggcgt gcatgcattg cgccgctatc cgaacggcga
    13321 ggagcgctgc atcgcgtgca agctctgcga ggcggtttgc ccggcgctgg cgatcaccat
    13381 cgattccgaa cagcgcgcgg acgacacccg ccgcaccacc cgctacgaga tcgacctgtt
    13441 caagtgcatc tactgcgggt tctgcgagga atcctgcccg gtggactcga tcgtcgagac
    13501 ccatgtccac gagtaccact tcgacaagcg cggccagaac atcgtgacca agccgcagtt
    13561 gctcgccatc ggcgaccgcc tggaggcgga gatcgccgag cgccgcgcgg ccgacgcgcc
    13621 gttccgttga ggttgacaga cgaatgaccc ccgatttccc gaccctggcc ttcctcggct
    13681 tcgccgccac cgcggcgatc gcggcactgg cgacgatcac cgcgcgcaac ccggtgcacg
    13741 ccgcgctgtg gctggtgctg accttcttct ccgtggcctg cacctggatc atcggcggcg
    13801 ccgagttcct cggcgtggcg ttggtgctgg tctatgtcgg tgcggtgatg gtgctgttcc
    13861 tgttcgtggt gatgatgctc gacgtcgaca tggcgccgct gcgcgagggc tacgtgcgct
    13921 acctgccggt cgggctggtg gtggcgctgg tgatgctggc gcagatcctg gcactgatcg
    13981 gggtgaaggc cgcggcggag gtgccgttcg cggacaacgc cgctgtcgcc gccgacgtct
    14041 ccaacaccac ctggctggcg cgcgcgctgt tcaccgaatt catgcttccg ttcgaggtgg
    14101 ccgcggtgat cctgacggtg gcagtggtgg ccgcggtgat gctgaccctg cgccgccgcg
    14161 gcggcaccaa gcaccaggat ccgtccgcgc aatcgcgggt cagggcaagc gaccgcctgc
    14221 gaatggtcaa gatggacgcg gagcggccgg cattgcccgc ggctgcgccg gaatcggagg
    14281 ccgcgccgtg agcgacatgc ccaatatctt cgcggcactg accctgggcc actacctggc
    14341 gctcggcgcg gtgctgttct gcatcagcgt cgccggcatc ttcctcaacc gcaagaacgt
    14401 gatcatgctg ctgatgtcgc tggagctgat gctgctggcg gtgaacatca acttcgtcgc
    14461 gttctcgcgt tcgctgggcg atgccgccgg ccaggtcttc gtgttcttca tcctgaccgt
    14521 ggccgccgcc gaatccgcga tcggcctggc gatcctggtc accctgttcc gcaaccgtcg
    14581 cacgatcgac gtggcggaca tcgacacctt gaagggctga ggcatggtga tttcgaagac
    14641 ccacctgctg ctggtcgtgc tgctgccgtt gctgggcgcg gtgctggccg gcctgttcgg
    14701 caggaagatc ggccgcgtcg gtgcgcacag cgcgaccatc ctcggggtgg cggcgagctg
    14761 cgtgctgtcg tgctgggtgc tatggcagct gttgcagggc gcggcgccgt tcaacgagaa
    14821 cgtctacacc tggttccagg tcggcggcat ccaggccaac gtcggcttca tggtcgacaa
    14881 gctgaccgcg atgatgatgg tggtggtcac cttcgtctcg ctgctggtgc acatctacac
    14941 catcggctac atggccgagg acgacggcta ccagcgcttc ttcagctaca tctcgctgtt
    15001 caccttcagc atgctgatgc tggtgatgag caacaacttc ctgcagctgt tcttcggctg
    15061 ggaagcggtg ggcctggtct cctacctgct gatcggcttc tggttcaaga agccgagcgc
    15121 gatcttcgcc aacctcaagg cgttcctggt caaccgcgtt ggcgacttcg ggttcctgct
    15181 ggggatcgcg ggcgtgctgt actggatcgg gaccctggac tacgccaccg cgttcgccaa
    15241 cgcgccgacc gtccgtgacg ccacggtgtc gctctgggcc ggcagcgagt ggtcggtgcc
    15301 caccgtcatc tgcatctgcc tgttcatcgg cgcgatgggc aagtccgcgc aggtgccgct
    15361 gcacgtgtgg ctgccggatt cgatggaagg cccgaccccg atctcggcgc tgatccacgc
    15421 cgcgaccatg gtcaccgccg gcatcttcat ggtggcgcgg atgtcgccgc tgttcgagat
    15481 gtcgccggcg gcgctcaact tcatcctgtt catcggcgcc accaccgcgt tctggaccgg
    15541 cctgatcggc atggtccaga acgacatcaa gcggg
//
LOCUS       DAORUK010000037        15436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_3591_length_15436_cov_17.914895, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000037 DAORUK010000000
VERSION     DAORUK010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15436
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3591_length_15436_cov_17.914895"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2392)
                     /gene="bamA"
                     /locus_tag="PK818_04015"
     CDS             complement(<1..2392)
                     /gene="bamA"
                     /locus_tag="PK818_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HQY81933.1"
                     /translation="MTRPLSRRLLTLALASALALPAWAQAAFAPFTVSDIRIEGLQRI
                     GAGTVFTYLPVERGDTLDQARAGEAMRALYKTGFFEDVRLDHQGSILVITVVERPAIN
                     KLTLVGNKDLKSEDLLKGLKEIGLAEGETFNKLNLDRVTQELNRQYNNRGKYSVEIEP
                     NVERLDRNRINLTLNIKEGKAAKIRHINLVGNESYSDEDITKAWESKTSNWMSWYKRD
                     DQYSREKLSGDIEKLNAWYLNRGHVDFSLDTTQVAISPDKKDMFLTAGITEGEVYTIS
                     GVTVSGDTVLPKEEIEQIVAFIKPGSTFSRGLLELATDAIAARLSNIGYAFAQVNPVP
                     AIDRDKRTVAIDMQVQPGPRVNVRRIVFKGNTRTADIVLRREMRQFEGSWYSQAAIDR
                     SKVRLSRLGYFEEVEVESEPVAGSDDQVDVVYTVKETTSGSIAAGVGYSQLSGVNLSL
                     QLSENNFLGTGNRVSMAVTSSTYQKRYDFSFMNPYFSDEGLSLGYNLWWREFDYSSYN
                     VAQYSTNSGAAQAFLGLPLSEADSVSLMFGIDSNEILAVPGSTPPPLIDYINAVGNRT
                     FKSWRTQLGWSRDTRNDFFVPVVGSTQSVSAEIALPGSTVEYYKVSFDFAKYWPLNPA
                     LVLRTGLNFGYGDSYGKDFVRNLCYTAPTLPTTGNPNPPPPPAPTDPCLITSPDYDKT
                     VTATGLPFFENFYAGGIAAQGKVRGFVDNTLGPAYTSPLGYRQPLGGSFLLAGSVEAI
                     FPKLFDSPAARISAFLDFGNVYDGWDNFSASELRASTGVALQWRSPMGPLSISYAFP"
     gene            complement(2463..3821)
                     /gene="rseP"
                     /locus_tag="PK818_04020"
     CDS             complement(2463..3821)
                     /gene="rseP"
                     /locus_tag="PK818_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HQY81934.1"
                     /translation="MTTFLGSLWWLLVALGVLVTFHEFGHFWVARRCGVKVLRFSVGF
                     GRALWSRRGKDGTEYVIAAIPLGGYVKMSDEREGEVPAAELDQAFNRQGVWKRIAIVA
                     AGPLANLLLAVALLWAMLVVGRPDYAPVVGKAEGIAAQAGLRAGDSIRAVDGRDTPTW
                     SEALMALTVAALDRRPVQVQVAHANGAESTHALALDRLPAGFDESRAVQAVGLTARHD
                     LLPAVVGKVSEGLPAWGVLAEGDRITAVDAHPVGSFDDIAPLVKALGERGGPGMVEVE
                     RDGERLALELTPARRGNPDGSTSWLLGVANAGVARPAPDAVLRLNPIAAIPAAIDETG
                     YQVQQLFAMIGRAFSGRVSVQNTVSGPIAIAQAANAYASGGVAWFLNFLALLSVSLAI
                     LNLLPIPVLDGGHLLYYLIELATGRPVGDRAMAIGQYLGLALIAGLTGLAFYNDILRL
                     VS"
     gene            complement(3818..5047)
                     /gene="dxr"
                     /locus_tag="PK818_04025"
     CDS             complement(3818..5047)
                     /gene="dxr"
                     /locus_tag="PK818_04025"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HQY81935.1"
                     /translation="MQRVAILGATGSIGASALDVVARHPGQMRASVLAAGSKVGELVA
                     LCRVHQPEHAVVADPARFADLRAGLVEAGLDTQPHAGGAALDALAASDACDSVVAAIV
                     GAAGLSSTLAAARAGKRLLLANKESLVLAGELLMQAAHAGGATIVPIDSEHNAVFQCW
                     SAAHGRADGGRHGSLEDPAAAVSRITLTASGGPFRGRPRATLAEVTPAQAVAHPKWSM
                     GPKISVDSATLMNKGLEVIEAHHLFGLDADRIRVLVHPQSLVHAIVDFVDGSSLAQLG
                     LPDMRTALAVGLGWPRRIASGVAPLDLLAQGGKLEFEPPDLDAFPCLALAWRALAAGG
                     TAPAALNAANEEAVSAFLQGRIGFLSIPDTVAATLDAMPTQPADSLDALLHADARARA
                     VALARIDALANRNHAQP"
     gene            complement(5064..5912)
                     /locus_tag="PK818_04030"
     CDS             complement(5064..5912)
                     /locus_tag="PK818_04030"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011258695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HQY81936.1"
                     /translation="MTKTRLLAALIMAPIAILSVLFVPTPVLAALAALLFLTALWEWL
                     KLAEVDDTLARTILLLCNVAVMAALVWGSRSAQGGSFALLQLVVVVGVAWWLLAMLWM
                     KHYHFASDHDSNARAFKLLAGTLAVVPAWCALGLIHASQPNGHQWLLLALLLVWSADT
                     GAYFAGRHFGGKFFARKLAPRISPNKTIEGLLGGLLLALAVAIAGALLIGARVEQLPA
                     IGVVALATVLFSVVGDLFESLLKRHVGAKDSGDLIPGHGGVLDRVDSVLAALPVFALG
                     KAWLGF"
     gene            complement(5909..6661)
                     /gene="uppS"
                     /locus_tag="PK818_04035"
     CDS             complement(5909..6661)
                     /gene="uppS"
                     /locus_tag="PK818_04035"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HQY81937.1"
                     /translation="MTVPATRVPRHVAIIMDGNGRWAQKRHRPRIIGHRAGARAVKAC
                     VEFCLASGVGALTLFAFSSENWNRPADEVGGLMKLFLGALEREVEELHRLGARLRFIG
                     DRSRFEPAILARMQAAEALTAGNASLQLSIAASYGGRQDIARAARALAEDVAAGRLRP
                     EQVDEDAISARVALAGLPPPDLFIRTGGELRISNFLLWQLAYTELWFTDTLWPDVDAA
                     TLRRALDDYAGRERRFGLTGAQVAGHATGGTT"
     gene            complement(6685..7242)
                     /gene="frr"
                     /locus_tag="PK818_04040"
     CDS             complement(6685..7242)
                     /gene="frr"
                     /locus_tag="PK818_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HQY81938.1"
                     /translation="MLNEIKKDAQARMGKSIDALRHTLIKLRTGRASAAIVDHLKVNY
                     YGSEVPLSQVASVAVTDARSITITPWEKQMVGPVEKAILASDLGLTPNTAGTVIRLNI
                     PALTEERRRDLSKVVHSEGEDAKVAIRNIRRDGNHQVKELLKDKQITEDEASRTEAEI
                     QKITDGAIRDVDEVVRAKEAELMAV"
     gene            complement(7334..8266)
                     /locus_tag="PK818_04045"
     CDS             complement(7334..8266)
                     /locus_tag="PK818_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQY81939.1"
                     /translation="MHGHPHADGTRAFAWVTLINLAYTLLEAGYGFATNSLALLSDAV
                     HNLGDVLGLALAWGAAVLARRPPTARHTYGWRRATLLSPLANALLLVAFSGALGWEAI
                     RRFSAPPEVPGLPVMVVAALGIAVNLGAAWLVRDGHAHDLNRRGAFLHLVADAAVSLA
                     AVLAGAGMWWLGWTWLDPAMALLIAVVVAAGSFGLLRDAFGAAMDAVPRGIDQAKVRE
                     WLLAQPGVSALHHLHIWSLGAGEIAMTAHLVRAGEADHDAFIDRVNDGLDAHFGINHP
                     TLQIERGTGCEHDCNDRAPHGHAHGHDDHDGHRH"
     gene            complement(8323..9045)
                     /gene="pyrH"
                     /locus_tag="PK818_04050"
     CDS             complement(8323..9045)
                     /gene="pyrH"
                     /locus_tag="PK818_04050"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010366166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HQY81940.1"
                     /translation="MSQLAYRRVLLKLSGEALMGDEDYGIDPKVIGRLAREVIEAREA
                     GAEIALVIGGGNIFRGAGLAAGGMDRVTGDQMGMLATVINALAMQDALEKLGAKCRVM
                     SALKINDVCEDYIRRRAVRHLEKGRIAIFAAGTGNPFFTTDSGAALRAIEIGADLLLK
                     ATKVDGVYDKDPKKHADAVKLDTLTYDDVLARNLQVMDTAAFALCRDAELPLRIFDMA
                     QPGVLLRILRGEQVGTLVHGRS"
     gene            complement(9170..10048)
                     /gene="tsf"
                     /locus_tag="PK818_04055"
     CDS             complement(9170..10048)
                     /gene="tsf"
                     /locus_tag="PK818_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HQY81941.1"
                     /translation="MAEITASLVKELRERTGAGMMECKKALTESNGDIDAAAEALRKS
                     GLAKADKKASRVAAEGRIAVAQAGGKAVLVEINSETDFVAKDANFVAFTDAVAQAALA
                     AGSTDVEALKAATLPGGETVEEARAAAIAKLGENILVRRIATIDTGNNVAAYSHGGKI
                     GVLVEVAGGDAEFARGVAMHVAAMNPPYNKAADVPAEFVAKEKEIELAKMSEKDKAKP
                     AEILEKIIGGKIAKIVNEVSLYGQPYVLNTDQSVEQAAKAAGADVVSFTRLAVGEGIE
                     KVVEDYAAEVAKAMQV"
     gene            complement(10186..11025)
                     /gene="rpsB"
                     /locus_tag="PK818_04060"
     CDS             complement(10186..11025)
                     /gene="rpsB"
                     /locus_tag="PK818_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011269915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HQY81942.1"
                     /translation="MPQITMRQMLEAGVHFGHQTRYWNPKMAPYIFGARGKIHIINLE
                     KTVPLFNDAMNFISGVAQKRGVILFVGTKRSARDPIKEEATRCGMPFMTQRWLGGSLT
                     NFATVKKSVARLKELEAAETDGTFQKLVKHEVMGLRRERDKLDASLGGIKDMNRLPDA
                     LFVIDIGHEDIAIKEAKKLGIPVIAVVDTNYDPSLVDYPIPGNDDAIRAVQLYAGAAA
                     DAVLEGKAAAPSAASVREEDFAGADGDDKKQGRRAPARKPAAPKADAAAPAAAEAAAP
                     KAE"
     gene            11278..12054
                     /gene="map"
                     /locus_tag="PK818_04065"
     CDS             11278..12054
                     /gene="map"
                     /locus_tag="PK818_04065"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HQY81943.1"
                     /translation="MSINLKTAADIEKMRQAGRLAAEVLQVVAPFVKPGVTTEELDRI
                     CHDHIVNVQGAIPANVGYRGYPKTVCTSVNNVICHGIPSAAKVLKDGDIVNIDVTVIK
                     DGWHGDTSRMYFVGTPSVMARRLVDVTREAMFRGIRVVRPGATLGDIGHAIQEFVERE
                     RFSVVREYCGHGIGKVYHDDPQVLHYGRPGEGVVLKEGMTFTIEPMINEGARHTRLLP
                     DGWTVVTKDRKLSAQWEHTVAVTADGVEILTRLPGDDNDL"
     gene            12051..14681
                     /gene="glnD"
                     /locus_tag="PK818_04070"
     CDS             12051..14681
                     /gene="glnD"
                     /locus_tag="PK818_04070"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HQY81944.1"
                     /translation="MSAPAAADHAPAPAEVPAGPAGDAAWAAATRALLAQVDARLAKR
                     FDGGEIVDRLVALRARAVDEMILDGWRRCIPADAGLALFAVGGYGRGELFPHSDIDLL
                     VLAESDAQQACAQSLARFLALLWDAGLQASQAVRSPAQCTEAAADQTVLTALIEARAL
                     VADADAQQRLRDAVSPECVWPARAFFEAKVREQAQRHARFGDTSDNLEPNLKDGPGGL
                     RDLHTLGWMALRSFGVRELDPLVGLDHLGADEAVALDRERRALGRLRFGLHLVAGRRE
                     ERLRFDYQKLLAERLGYLDDADSLAVEKMMQGFYRSAAIVRRINDRLLQRFEEHFDGA
                     AEPQPLDDDLELRRGYLASRRPGWLLEDTGHVFALFAAWAADRGVRGLHSQTARELAR
                     ALPGISDYTRASPRLREAFMQLLRGPQPVKTLERMTRLGVLGRWIPAFAQVSGRMQFD
                     LFHVYTVDQHTLAVLRNLAAFASGDADERFAVAHTVWPALRKPELLLLAGLFHDIGKG
                     RGGDHSELGAVDARAFCEAMGLSEADTALVEWLVRRHLLMSVTAQKQDITDPEVIHRF
                     ATVVADRQRLDYLYLLTCADIAGTSPKLWNAWKDRLLADLHTATRLALRRGLEHPVAA
                     DQRIGENRDAARALLVAEGHGDPAIDAMFARMPRDNFLRSRPEQIAWQANALRAAGAG
                     EVVARARQLPGGGYEVLVHAPDRDGLFAAIVIALDRAGLAILQARALDGPDATIFDSF
                     HVLPNDLRHAPAPAQIEAGLQRVLAGDLRRVAPARRSQPRHLRHFRIVPQVGFADADA
                     ERTLVSIVCNDRPGLLADIARVLREQRLRVHDARIATFGERAEDMFRISDEHDRPLHD
                     DHQRQALRDALLAALDGDPR"
     gene            14678..>15436
                     /locus_tag="PK818_04075"
     CDS             14678..>15436
                     /locus_tag="PK818_04075"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HQY81945.1"
                     /translation="MSPAKPKKTAAEAPARPSPAKARATARAAELETLRFEIESAWER
                     RAMLTPEELEGATRPAVERAIEGLEKGRLRVAEPDGNGGWRVNEWLKKAVLLYFRTQQ
                     MELVEAYPAPFWDKVPARFAGFDEADFRKLGVRVVPGAVVRAGSHVGKDVVLMPSFVN
                     IGAFVGEGTMVDTWATVGSCAQVGKHCHLSGGAGIGGVLEPLQASPTIIEDHCFIGAR
                     SEVVEGFVVGHHSVVGMGVFLGQSTRVYNRATGEV"
BASE COUNT         2257 a         5304 c         5510 g         2365 t
ORIGIN      
        1 gcgggaacgc gtagctgatc gacagcggac ccatcggcga gcgccactgc agggcaacgc
       61 cggtggaggc gcgcaattcg ctggcgctga agttgtccca gccgtcgtag acgttgccga
      121 agtccaggaa cgcggaaatc cgtgccgccg ggctgtcgaa cagcttcggg aagatcgcct
      181 ccaccgagcc ggccagcagg aacgacccac ccagcggctg gcgatagccc aggggtgacg
      241 tataggcagg ccccagcgtg ttgtcgacga agccgcggac cttgccctgg gcggcgatgc
      301 cgcctgcgta gaagttctcg aagaacggca acccggtggc ggtcaccgtc ttgtcgtagt
      361 ccggcgacgt gatcaggcag gggtcggtgg gcgccggcgg cggtggcggg ttggggttcc
      421 cggtggtcgg cagggtcggc gcggtgtagc agaggttgcg cacgaaatcc ttgccgtagg
      481 aatcgccgta gccgaagttg agcccggtgc gcaggaccag cgccgggttg agcggccagt
      541 acttggcgaa atcgaaggac accttgtaat actcgacggt ggatcccggc aacgcgattt
      601 ccgccgacac gctctgggtg ctgccgacca ccgggacgaa gaaatcgttg cgggtgtcgc
      661 gcgaccagcc cagctgggtc cgccaggact tgaaggtgcg gttgcccacc gcgttgatgt
      721 agtcgatcag tggcggcggg gtggaaccgg gaaccgcgag gatctcgttg gaatcgatcc
      781 cgaacatcag cgagaccgaa tcggcttcgc tcagcggcag gccgaggaag gcctgcgccg
      841 cgccgctgtt ggtcgagtac tgggcgacgt tgtagctgga atagtcgaat tcgcgccacc
      901 agaggttgta cccgagcgac aggccctcgt cggagaagta cgggttcatg aacgagaagt
      961 cgtagcgctt ctggtaggtg ctgctggtca ccgccatcga cacccggttg ccggtgccga
     1021 ggaaattgtt ctccgacagc tgcagcgaca ggttgacgcc cgacagctgc gaatagccga
     1081 cgccggccgc gatgctgccc gaggtggttt ccttcaccgt gtacaccacg tcgacctggt
     1141 cgtcgctgcc ggcgaccggt tcgctctcga cttccacttc ctcgaagtac ccgagccgcg
     1201 acaggcggac cttgctgcgg tcgatcgcgg cctgcgaata ccagctgccc tcgaactggc
     1261 gcatttcgcg gcgcagcacg atgtcggcgg tgcgggtgtt gcccttgaac acgatccggc
     1321 gcacgttgac gcgcggcccg ggttgcacct gcatgtcgat ggcgacggtg cgcttgtcgc
     1381 ggtcgatcgc cggtaccggg ttcacctggg cgaaggcata gccgatgttg gacaggcgcg
     1441 cggcgatcgc gtcggtcgcc agctccagca ggccgcgcga gaaggtgctg ccgggcttga
     1501 tgaaggcgac gatctgctcg atctcttcct tcggcagcac ggtgtcgccg gaaacggtga
     1561 cgccggagat cgtgtagacc tcgccttcgg tgatcccggc ggtgaggaac atgtccttct
     1621 tgtcggggct gatcgcgacc tgggtggtgt ccaggctgaa atcgacgtgg ccgcggttga
     1681 ggtaccacgc gttcagcttc tcgatgtcgc cggacagctt ctcgcgcgag tactggtcgt
     1741 cgcgcttgta ccaggacatc cagttgctgg tcttggactc ccacgccttg gtgatgtcct
     1801 cgtcgctgta gctctcgttg ccgaccaggt tgatgtggcg gatcttggcg gccttgcctt
     1861 ccttgatgtt cagggtcagg ttgatccggt tgcggtccag ccgctccacg ttcggttcga
     1921 tctcgacgct gtacttgccg cggttgttgt actggcggtt cagctcctgg gtgacccggt
     1981 ccaggttgag cttgttgaag gtctcgcctt cggccagtcc gatttccttc aggcccttga
     2041 gcaggtcctc cgacttcagg tccttgttgc cgaccagggt cagcttgttg atcgccgggc
     2101 gctcgaccac ggtgatcacc aggatgctgc cctggtggtc caggcgcacg tcctcgaaga
     2161 agccggtctt gtacagcgcg cgcatggcct cgccggcgcg ggcctggtcg agggtgtcgc
     2221 cgcgctcgac cggcaggtag gtgaagaccg tgccggcgcc gatgcgctgc aggccttcga
     2281 tgcggatgtc gctgaccgtg aacggtgcga acgccgcctg tgcccaggct ggcagcgcaa
     2341 gcgccgaagc cagggcaagg gtaagcaggc ggcgggacaa gggtcgggtc attcggaaca
     2401 tccggttgcg gaacgtggtg ctgggcgggc gcgggtggcg ccagcggcgg gggagcgacg
     2461 cgtcacgaca ccaggcgcag gatgtcgttg tagaacgcca gccccgtcag ccccgcgatc
     2521 aacgcgaggc cgaggtactg gccgatggcc atcgcgcgat cgcccacggg gcggccggtg
     2581 gccagctcga taaggtaata cagcaggtga ccaccgtcca agacgggaat cggcagcagg
     2641 ttgaggatcg caaggctgac cgacagcagg gccaggaagt tcaggaacca cgccacgccg
     2701 ccgctggcat aggcattcgc ggcctgggcg atggcgatcg gcccggagac cgtgttctgc
     2761 accgaaaccc ggccgctgaa cgcgcgccca atcatcgcga acaactgctg cacctggtag
     2821 ccggtttcgt cgatcgccgc cgggatcgcg gcgatcgggt tgagccgcag gactgcgtcg
     2881 ggcgccggcc gggcgacgcc cgcattggcc accccgagca gccagctggt gctgccgtcg
     2941 gggttgccgc gccgcgccgg ggtcagctcc agcgccagcc gctcaccgtc gcgttcgacc
     3001 tcgaccatgc ccgggccgcc gcgttcgccg agcgccttca ccagcggggc gatgtcgtcg
     3061 aagctgccga ccggatgggc gtcgaccgcg gtgatgcggt cgccttcggc cagcacgccc
     3121 caggccggca ggccctcgct gaccttgccg accaccgccg gcagcaggtc gtggcgggcg
     3181 gtcaggccaa ccgcctgcac cgcgcgcgat tcgtcgaagc ccgcaggcag gcggtccagc
     3241 gccagcgcgt gggtggattc ggcgccgttg gcgtgggcga cctgcacctg caccggcctg
     3301 cggtcgagcg cggccaccgt cagcgccatc agcgcctcgc tccaggtcgg ggtgtcgcgg
     3361 ccgtcgaccg cacggatcga atcgcccgcg cgcagcccgg cctgcgcggc gatgccctcg
     3421 gccttgccga ccaccggggc gtagtccggg cggccgacca ccagcatcgc ccacagcaac
     3481 gcgaccgcca gcagcaggtt ggccagcggg ccggcagcca cgatcgcgat ccgcttccag
     3541 acgccctggc ggttgaaggc ctggtcgagc tcagccgcgg ggacctcgcc ctcgcgttcg
     3601 tccgacatct tcacgtagcc gccaagcggg atcgcggcga tcacgtactc ggtgccgtcc
     3661 ttgccgcgcc gcgaccacag cgcgcggcca aagccgaccg agaagcgcag caccttgacg
     3721 ccgcagcggc gggcgaccca gaaatggccg aactcgtgga aggtcaccag cacccccagc
     3781 gccaccagca gccaccacag cgatccgagg aaggtggtca tggctgcgcg tggttccggt
     3841 tggcaagggc gtcgatgcgc gccagcgcca cggcgcgcgc gcgcgcgtcc gcgtgcagca
     3901 gcgcgtccag cgaatcggcg ggctgggtgg gcatggcgtc cagggtcgcg gcgacggtgt
     3961 cgggaatcga caggaaaccg atccggccct gaagaaaggc tgaaacggcc tcctcgttgg
     4021 cggcgttgag cgccgccggg gcggtgccgc cggccgccag cgcgcgccag gccagggcca
     4081 ggcaggggaa ggcgtcgagg tcgggcggct cgaactccag cttgccgccc tgcgccagca
     4141 ggtcgagcgg ggcgaccccg gaggcgatcc gccgcggcca gcccaggccc accgccagcg
     4201 cggtgcgcat gtccggcagc cccagctgcg ccagcgaact gccgtccacg aaatcgacga
     4261 tggcgtgcac cagcgactgc ggatgcacca gcacccggat ccggtccgca tccagcccga
     4321 acaggtggtg ggcctcgatg acctccaggc ccttgttcat cagggtggcc gaatccaccg
     4381 agatcttcgg ccccatcgac catttcgggt gcgccactgc ctgcgcgggg gtgacctcgg
     4441 caagcgttgc ccgtggcctg ccgcggaacg ggccgccgga cgcggtcagg gtgatccgcg
     4501 ataccgccgc cgccggatcc tcgagcgaac cgtggcgccc gccatcggcg cgcccgtgcg
     4561 cggccgacca gcactggaac accgcattgt gctcgctgtc gatcggcacg atggttgcgc
     4621 cgcctgcgtg cgcggcctgc atcagcagct cgcccgccag caccagcgat tccttgttgg
     4681 ccagcagcag gcgcttgccg gcgcgcgccg ctgccagtgt ggaggacagc ccggcggcgc
     4741 cgacgatcgc cgcgaccacg ctgtcgcagg cgtcgctggc ggccagtgcg tcgagggcgg
     4801 cgccgcctgc gtgcggctgg gtgtccagcc ctgcctccac caagcccgcg cgcaggtccg
     4861 cgaagcgcgc cggatcggcg accaccgcgt gttccggttg gtgcacgcgg cacagcgcga
     4921 ccagttcgcc gaccttgctg ccggcggcca gcacgctggc gcgcatctgc ccgggatggc
     4981 gcgcgaccac gtccagcgcc gaggcgccga tcgaaccggt ggcgccgaga atcgcgaccc
     5041 gctgcatggc ctggcgcttc cccctagaac ccgagccagg ccttgcccag cgcgaacacc
     5101 ggcagcgcgg ccagcacgct gtccacgcgg tccagcacgc cgccgtggcc ggggatcagg
     5161 tcgccggaat ccttcgcccc gacgtggcgc ttgagcaggc tctcgaacag gtcgccgacc
     5221 accgagaaca gcaccgtcgc cagcgccacc accccgatcg ccggcaactg ctcgacgcgc
     5281 gcgccgatca gcaacgcacc ggcgatcgcc accgccagcg ccagcaacag gccgccaagc
     5341 aggccttcga tggtcttgtt ggggctgatc cgtggcgcca gcttgcgggc gaagaatttc
     5401 ccgccgaagt ggcggccggc gaaatacgca ccggtatccg cggaccagac cagcaacagc
     5461 gccagcagca gccactggtg cccgttcggc tggctggcat ggatcaggcc cagcgcgcac
     5521 caggccggga ccacggccag ggtgccggcg agcagcttga aggcgcgcgc gttcgaatcg
     5581 tggtcggagg cgaagtggta gtgcttcatc cacagcatcg ccagcagcca ccaggccacg
     5641 ccgaccacca ccaccagctg cagcagcgcg aacgagccgc cctgcgccga acgcgagccc
     5701 cacaccagcg ccgccatcac cgcgacgttg cacagcagca ggatggtgcg ggcgagggtg
     5761 tcgtcgacct cggccagctt cagccattcc catagcgcgg tcaggaacag cagcgcagcc
     5821 agcgccgcaa gcaccggggt gggcacgaac agcacgctga ggatggcgat cggcgccatg
     5881 atcagcgcgg ccagcaggcg cgtcttggtc acgtggttcc cccggttgcg tggcccgcca
     5941 cctgcgcgcc ggtcagcccg aagcggcgtt cgcgaccggc gtaatcgtcc agtgcgcggc
     6001 gcagtgtggc ggcatccacg tccggccaca acgtgtcggt gaaccacagc tcggtatagg
     6061 cgagctgcca cagcaggaag ttgctgatcc gcagctcgcc gccggtgcgg atgaacaggt
     6121 cgggcggcgg cagcccggcc agcgccaccc gcgcggagat cgcgtcctcg tccacctgct
     6181 ccggacgcag ccggccggcg gccacgtcct cggccagcgc ccgcgcggca cgggcgatgt
     6241 cctggcggcc gccgtagctg gcggcgatgc tcagctgcag gctcgcgttg ccggcggtca
     6301 gtgcttccgc ggcctgcatg cgggcgagga tggccggctc gaagcggctg cgatcaccga
     6361 tgaagcgcag ccgcgccccg agccggtgca gttcctcgac ctcgcgctcc agcgcgccga
     6421 ggaacagctt catcagcccg ccgacctcgt ccgccgggcg gttccagttc tcgctggaaa
     6481 atgcgaacag ggtcagtgcc ccgaccccgc tggccaggca gaactccacg caggccttga
     6541 ccgcgcgcgc accggcgcgg tggccgatga tgcgcgggcg atggcgcttc tgcgcccagc
     6601 ggccgttgcc atccatgatg atggcgacat ggcgcggaac gcgcgttgcg ggaaccgtca
     6661 cggcagggac gtcgcgggcg cggttcaaac cgccatcagt tccgcttcct tggccctcac
     6721 cacttcatcc acgtcccgga tcgcgccgtc ggtgatcttc tggatctccg cctcggtgcg
     6781 gctggcttcg tcctcggtga tctgcttgtc cttcagcagt tccttgacct ggtggttgcc
     6841 atcgcggcgg atgttgcgga tcgcgacctt ggcgtcctcg ccctcgctgt gcacgacctt
     6901 ggacaggtcg cggcgccgtt cctcggtcag cgccgggatg ttgaggcgga tcacggtgcc
     6961 cgcggtgttc ggggtcaggc cgaggtcgga ggccaggatc gccttttcga ccgggcccac
     7021 catctgcttt tcccacgggg tgatggtgat cgagcgggcg tcggtgaccg ccacgctggc
     7081 gacctgcgac agcggcacct cggagccgta gtaattgacc ttcaggtggt cgacgatcgc
     7141 cgcgctggcg cgcccggtgc gcagcttgat cagcgtgtgg cgcagggcgt cgatgctctt
     7201 gcccatgcgg gcctgggcat ccttcttgat ttcgttcagc atcgtgtcgc tccgggttgc
     7261 tgggtaaccg cgggattata tgcgaggccg catcgcggcc agcgccgcgg gccggcgacc
     7321 gtggatgcag gcgctagtgg cggtgaccgt cgtggtcgtc gtgaccgtgc gcatgcccgt
     7381 gcggcgcgcg gtcgttgcag tcgtgctcgc agccggtgcc ccgctcgatc tgcagggtgg
     7441 ggtggttgat cccgaagtgg gcgtcgaggc cgtcgttgac gcggtcgatg aaggcgtcgt
     7501 ggtcggcttc gccggcgcgc accaggtgcg cggtcatcgc gatctcgccc gcgcccagcg
     7561 accagatgtg caggtggtgc agggcggaca cgccgggttg cgcgagcagc cattcgcgca
     7621 ccttcgcctg gtcgatgccg cgcggcaccg catccatcgc cgcgccgaag gcatcgcgca
     7681 gcagcccgaa cgacccggcc gcgaccacca ccgcgatcag cagcgccatc gccggatcca
     7741 gccaggtcca gcccagccac cacatgccgg cgccggccag caccgccgcc agcgagaccg
     7801 cggcatcggc gaccaggtgc aggaacgccc cgcgccggtt gaggtcgtgg gcatggccgt
     7861 cgcgcaccag ccaggccgcg ccgaggttga ccgcgatgcc gagggcggcg accaccatca
     7921 ccggcagccc ggggacctcg ggcggggcgc tgaagcggcg gatcgcctcc cagccgagcg
     7981 cgccggagaa cgcgaccagc agcagcgcat tggccagcgg cgagagcagg gtggcccggc
     8041 gccagccgta ggtatggcgc gctgtcggcg ggcgccgggc gaggaccgcg gccccccacg
     8101 ccagcgccag cccgagcacg tcgccgaggt tgtgcacggc gtccgacagc agcgccagcg
     8161 aattggtggc gaagccgtag ccggcctcca gcaacgtgta ggcgaggttg atcagggtca
     8221 cccacgcgaa cgcgcgggtg ccatctgcgt gcgggtggcc atgcatggcg ctagggtgcc
     8281 cggcgcgcgg cgcggacaca atcacggtcc gcggtggcgc gctcagctgc ggccgtggac
     8341 cagggttccg acctgctcgc cgcgcaggat ccgcagcagc acgcccggtt gcgccatgtc
     8401 gaagatccgc agcggcagtt cggcatcgcg gcacagcgcg aacgcggcgg tgtccatcac
     8461 ctgcaggttg cgggcgagca cgtcgtcgta ggtcagcgtg tccagcttga ccgcgtcggc
     8521 gtgcttcttc gggtccttgt cgtacacgcc atcgaccttg gtcgccttca gcagcaggtc
     8581 ggcgccgatc tcgatcgcac gcagcgcggc gccggaatcg gtggtgaaga acgggttgcc
     8641 ggtgccggcg gcgaagatcg cgatgcggcc cttctccagg tggcgcacgg cgcggcggcg
     8701 gatgtagtcc tcgcacacgt cgttgatctt gagcgcgctc atgacccggc acttggcgcc
     8761 cagtttttcc agcgcgtcct gcatcgccag cgcgttgatc accgtggcca gcatgcccat
     8821 ctggtcgccg gtgacgcggt ccatgccgcc ggcggccagc ccggcgccgc ggaagatgtt
     8881 gccgccgccg atcaccagcg cgatctcggc gccggcctcg cgcgcctcga tcacctcgcg
     8941 cgccagccgg ccgatcacct tcgggtcgat cccgtagtcc tcgtccccca tcagggcttc
     9001 gccggagagc ttgagcagga cgcggcggta ggcgagttgc gacatgcgaa gggctccagg
     9061 cgggcgggca aacagcggaa ttctagccga tcgccgggcg ggacccgggc cggccaacaa
     9121 aaaacccgcc gtcgccggcg ggtttttcgc gtcgcgatgg tgcggtggat cagacctgca
     9181 tcgccttcgc cacttccgcg gcgtagtcct cgaccacctt ctcgatgcct tcgcccaccg
     9241 ccaggcgggt gaaggacacc acgtcggcgc cggcggcctt ggcggcctgc tcgacgctct
     9301 ggtcggtgtt cagcacgtac ggctggccgt acaggctgac ctcgttgacg atcttggcga
     9361 tcttgccgcc gatgatcttc tccaggatct cggccggctt ggccttgtcc ttctcggaca
     9421 tcttggccag ctcgatttcc ttttccttcg ccacgaactc ggccgggacg tccgcggcct
     9481 tgttgtaggg cgggttcatc gcggccacgt gcatcgccac gccgcgggcg aactcggcgt
     9541 cgccgccggc cacttcgacc agcacgccga tcttgccgcc gtgggagtag gcggccacgt
     9601 tgttgccggt gtcgatggtg gcgatgcggc ggaccaggat gttctcgccg agcttggcga
     9661 tggcggcggc gcgcgcttcc tcgacggtct cgccgcccgg cagggtcgcg gccttgaggg
     9721 cttcgacgtc ggtgctgccc gcggccagcg cggcctgggc gacggcgtcg gtgaaggcga
     9781 cgaagttggc gtccttggcg acgaagtcgg tctccgagtt gatctcgacc aggaccgcct
     9841 tgccacccgc ctgggcgacc gcgatgcggc cttcggcggc gacgcggctg gccttcttgt
     9901 cggccttggc gaggccggac ttgcgcaggg cctccgcggc ggcgtcgatg tcgccgttgc
     9961 tttcggtgag cgccttcttg cactccatca tgccggcgcc ggtgcgctcg cgcagttcct
    10021 tgaccaggga agcggtgatt tcagccatgg gaattacctc gatgttcgat gtaggagcgc
    10081 ccgatgggcg cgaacggcag gcgccggcgc cagcgcacgc ccatcgggcg ctcctacgga
    10141 aggggaagcc gccgcgcagc gtggcgcggc ggcgtgacgc gggtgttact cggccttcgg
    10201 cgcggcggcc tcggcggcgg caggtgccgc tgcatcggcc ttcggcgcgg ccggcttgcg
    10261 ggccggggcg cggcggccct gcttcttgtc gtcgccgtcg gcgccggcga agtcttcctc
    10321 gcgcacgctg gcggcggacg gcgcggcggc cttgccttcc agcacggcgt cggcggcggc
    10381 gcctgcatac agctgcacgg cgcggatggc gtcgtcgttg ccggggatgg ggtagtcgac
    10441 caggctgggg tcgtagttgg tgtcgaccac cgcgatcacc gggatgccga gcttcttggc
    10501 ttccttgatc gcgatgtcct catggccgat gtcgatcacg aacagggcgt cgggcaggcg
    10561 gttcatgtcc ttgatgccgc ccagcgaggc gtccagcttg tcgcgctcgc ggcgcaggcc
    10621 catcacttcg tgcttgacca gcttctggaa ggtgccgtcg gtttccgcgg cttccagttc
    10681 cttcaggcgg gcgaccgact tcttcacggt ggcgaagttg gtcagcgagc cgcccagcca
    10741 gcgctgggtc atgaacggca tgccgcaacg ggtggcttct tccttgatcg ggtcgcgcgc
    10801 gctgcgcttg gtgccgacga acaggatcac gccgcgcttc tgggccacgc cgctgatgaa
    10861 gttcatcgcg tcgttgaaca gcgggacggt cttctcgagg ttgatgatgt ggatcttgcc
    10921 gcgggcaccg aagatgtacg gcgccatctt ggggttccag tagcgggtct ggtggccgaa
    10981 atggacgccg gcttccagca tctggcgcat ggtgatctgg ggcattgcaa aactccgatg
    11041 ggtggaatcc gcgccggctt ggatgaaggc gctgcgccgc atgccgcgtg gcatggagcg
    11101 ctggatcccg gggttgggcc tccctgtcgt cactgcgtcc gaactccttg cggagcaccc
    11161 cggcgcaggg gttgtcgaca ggtgtggatt tgtcggtagc gcccgacatg gcggcggtaa
    11221 agccgcggaa gtatagcccg cggcgcggat tccgggcaaa tcggcgataa tccgggcatg
    11281 agcatcaacc tgaaaaccgc cgccgacata gagaagatgc gccaggccgg ccgcctggcc
    11341 gccgaagtgc tgcaggtggt cgcgccgttc gtgaagcccg gcgtgaccac cgaggaactg
    11401 gaccgcatct gccacgacca tatcgtcaac gtgcagggcg cgatccccgc caacgtcggc
    11461 taccgcggct atccgaagac cgtgtgcacc tcggtcaaca acgtgatctg ccacggcatc
    11521 ccgagcgccg ccaaggtcct gaaggacggc gacatcgtca acatcgacgt caccgtcatc
    11581 aaggacggct ggcacggcga taccagccgc atgtacttcg tcggtacccc gtcggtgatg
    11641 gccaggcggc tggtggacgt cacccgcgag gccatgttcc gcggcatccg ggtggtcagg
    11701 ccgggcgcga ccctcggcga catcggccac gcgatccagg aattcgtcga gcgcgagcgc
    11761 ttcagcgtgg tccgcgagta ctgcgggcac ggcatcggca aggtctacca cgacgacccg
    11821 caggtgctcc actacggccg acccggcgag ggcgtggtgc tgaaggaagg catgaccttc
    11881 accatcgagc cgatgatcaa cgagggcgcc cgccacaccc ggctgctgcc ggacggctgg
    11941 accgtggtca ccaaggaccg caagctgtcc gcgcagtggg agcacacggt ggcggtgacc
    12001 gccgacggcg tggagatcct gacccgcctg cccggcgacg acaacgacct gtgagcgcgc
    12061 ctgcggcagc cgaccacgcc ccggctcccg ctgaagtccc cgcgggtccg gcgggcgatg
    12121 ccgcctgggc cgccgccacc cgcgcgctgc tggcccaggt cgacgccagg ctggcgaaac
    12181 gcttcgatgg cggcgagatc gtcgacaggc tggtcgcgct gcgcgcccgc gccgtcgatg
    12241 aaatgatcct cgacggctgg cggcgctgca ttcccgcgga tgccggtctt gccctgttcg
    12301 cggtcggcgg ctacggccgc ggcgaattgt tcccgcattc cgacatcgac ctgctggtgc
    12361 tggccgaaag cgacgcgcag caggcctgcg cgcagtcgct ggcgcgcttc ctcgcgctgt
    12421 tgtgggatgc cggcctgcag gccagccagg cggtgcgctc gcccgcgcag tgcaccgagg
    12481 ccgccgccga ccagaccgtg ctcacggcgc tgatcgaggc gcgcgcgctg gtcgccgacg
    12541 cggacgcgca acagcgcctg cgcgacgcgg tctcgcccga atgcgtgtgg cccgcgcgtg
    12601 ccttcttcga ggccaaggtg cgcgaacagg cgcagcggca cgcgcgcttc ggcgatacca
    12661 gcgacaacct cgagccgaac ctcaaggacg gccccggcgg cctgcgcgac ctgcacaccc
    12721 tgggctggat ggcgctgcgc agcttcggcg tgcgcgaact ggatccgctg gtcgggctgg
    12781 accaccttgg cgccgacgag gcggtggcgc tggatcgcga acgccgcgcg ctgggccggc
    12841 tgcgtttcgg cctgcacctg gtcgccggtc ggcgcgagga gcggctgcgc ttcgattacc
    12901 agaagctgct ggccgaacgc ctgggctacc tcgacgatgc cgattcgctg gcggtcgaga
    12961 agatgatgca gggcttctac cgcagcgcgg cgatcgtgcg gcggatcaac gaccgcctgc
    13021 tgcagcgctt cgaggaacac ttcgacggtg ccgccgaacc gcaaccgctg gacgacgatc
    13081 tcgaattgcg gcgcggctac ctcgcctcgc gcaggccggg ctggctgctg gaggacaccg
    13141 gccacgtgtt cgcgctgttc gcggcgtggg ccgcggaccg cggcgtccgc ggcctgcatt
    13201 cgcagaccgc gcgcgagctg gcgcgtgcgt tgccggggat cagcgactac accagggcat
    13261 cgccacgctt gcgcgaagcc ttcatgcaac tgctgcgcgg cccgcagccg gtgaagacgc
    13321 tggaacggat gacccggctc ggcgtgctcg ggcgctggat ccccgcgttc gcgcaggtct
    13381 ccgggcgcat gcagttcgac ctgttccacg tgtacacggt ggaccagcac acgctggcgg
    13441 tgctgcgcaa cctggcggcg ttcgcctcgg gcgacgcgga cgaacgcttc gcggttgcgc
    13501 acacggtgtg gccggccttg cgcaagccgg aactgttgct gctggccggg ctgttccacg
    13561 acatcggcaa gggccgcggc ggcgaccatt ccgaactcgg cgcggtggat gcgcgcgcgt
    13621 tctgcgaggc gatggggctg tcggaggccg acaccgcgct ggtcgaatgg ctggtgcgca
    13681 ggcacctgct gatgtcggtg accgcgcaga aacaggacat caccgacccg gaggtgatcc
    13741 accgcttcgc cacggtggtc gccgaccgcc agcgcctgga ctacctgtac ctgctgacct
    13801 gcgcggacat cgccggcacc tcgcccaagc tctggaacgc gtggaaggac cggctgctgg
    13861 ccgacctgca caccgccacc cggctggcgt tgcggcgcgg cctggagcac ccggtcgcgg
    13921 ccgaccagcg gatcggggag aaccgtgatg cggcgcgcgc gctgctggtc gcggaagggc
    13981 atggcgatcc ggcgatcgac gcgatgttcg cgcggatgcc gcgcgacaat ttcctgcgca
    14041 gccggccgga gcagatcgcc tggcaggcga acgcactgcg cgcggccggc gccggcgagg
    14101 tggtcgcgcg cgcgcgccag ttgcccggcg gcggctacga agtgctggtg catgcgccgg
    14161 accgcgacgg cctgttcgcg gccatcgtga tcgcgctgga ccgcgccggc ctggcgatcc
    14221 tgcaggcgcg cgcgctggac ggtcccgacg ccaccatctt cgacagcttc cacgtgctgc
    14281 ccaatgacct gcggcatgcg ccggcgccgg cgcagatcga ggccgggttg cagcgcgtgc
    14341 tcgccggcga cctccgccgg gtggcgccgg cgcggcgcag ccagccgcgg cacctgcgcc
    14401 atttccggat cgtgccgcag gtgggcttcg ccgacgccga tgccgaacgc accctggtca
    14461 gcatcgtctg caacgatcgc ccgggcctgc tcgccgacat cgcccgggtg ctgcgcgagc
    14521 agcgcctgcg cgtccacgac gcgcgcatcg ccaccttcgg cgagcgcgcc gaggacatgt
    14581 tccggatcag cgacgagcac gaccgcccgc tgcatgacga ccaccaacgc caggcgttgc
    14641 gcgatgcgct gctggccgca ctcgatggag acccgcgatg agccccgcca agccgaagaa
    14701 gaccgccgcc gaggcccccg ccaggccgtc acccgcgaag gcgcgcgcca ccgcgcgcgc
    14761 cgcggaactg gagaccctgc gcttcgagat cgagagcgcg tgggaacgcc gcgccatgct
    14821 gaccccggaa gagcttgagg gcgccacccg gccggcggtc gagcgcgcca tcgaaggcct
    14881 ggagaagggc cgcctgcggg tggcggagcc ggacggcaac ggcggctggc gggtcaacga
    14941 atggctgaag aaggcggtgc tgctgtattt ccgcacccag cagatggagc tggtcgaagc
    15001 ctacccggcg ccgttctggg acaaggtgcc ggcgcgtttc gcgggcttcg acgaggcgga
    15061 tttccgcaag ctcggcgtgc gcgtggtgcc gggtgcggtg gtgcgcgcgg gcagccacgt
    15121 cggcaaggac gtggtgctga tgccgagctt cgtcaacatc ggcgcgttcg tgggcgaagg
    15181 caccatggtc gatacctggg ccacggtcgg cagctgcgcg caggtcggca agcactgcca
    15241 cctgtccggc ggcgccggca tcggcggggt gctggagccg ctgcaggcca gcccgacgat
    15301 catcgaggac cattgcttca tcggtgcgcg ctccgaggtg gtggaggggt tcgtggtcgg
    15361 ccaccacagc gtggtcggca tgggcgtgtt cctgggccag agcacgcgcg tctacaaccg
    15421 cgccaccggc gaggtc
//
LOCUS       DAORUK010000038        15329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_3629_length_15329_cov_18.232421, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000038 DAORUK010000000
VERSION     DAORUK010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15329
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3629_length_15329_cov_18.232421"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PK818_04080"
     CDS             <1..302
                     /locus_tag="PK818_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HQY81946.1"
                     /translation="MLAALLCEVVLTAMFLVVILGATHKNAAPGFAPIAIGLGLTLIH
                     LISIPVTNTSVNPARSTGVALMVGGTPLSQLWLFWVAPIVGGLLGGVVYKWLGRD"
     gene            complement(374..1129)
                     /locus_tag="PK818_04085"
     CDS             complement(374..1129)
                     /locus_tag="PK818_04085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HQY81947.1"
                     /translation="MRLLPSLLALALASLLPTTVLAQQAPADAEPAETWDVAGSVVAT
                     SDYVWRGVSQTQGNPALQLDVSVTHASGFYAGAWASNVDFTAAGEDDDGIDIELDPYI
                     GWSGALGENAELDVSLTYVSYPGHLDGYDYAYTELEGVLTFADHFHVGLAYSPDIFNL
                     GGHGIYGNAGVELPLGESGFDLKAQVGHYDLNDAVGDSYNDFQVGLGRSFGPLRADLL
                     YTDTSSYGEALSEGLDDASQADGRVSLQLTWEF"
     gene            1241..2395
                     /locus_tag="PK818_04090"
     CDS             1241..2395
                     /locus_tag="PK818_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HQY81948.1"
                     /translation="MTLRLAPLACAIALLAACGGKPAAEPATAGTAAQAPEEKVLNVY
                     NWSDYVAEDTIANFEKATGIKVNYDVYSENETLETKLTAGSSGYDVVFPSARPFAQRQ
                     VKGGLYLALDKARLPNLSHLDPAIMQGLQDVDPANAHVVPYMWGTTGLGINVDKVKAA
                     LGDGAPIDSWNLLFDPANAARLAKCGISVLDDDQEAFGAALIWKGKDPNAGAADEIET
                     VKQVYAAIRPHIRTFNNAEYKDALANGDACVVMGYSGDILAAADAASEAAEAAGKPAP
                     DIRYVIPKEGALRWVDVIAIPKDARHPGNALAFIDYLLQPAVTAAITDAVGYANPNSD
                     ARALVDPGIAGNPGIYPAAEVQARLVDPKSLPEAVQRQRVRAWTAIKSGR"
     gene            2399..3550
                     /locus_tag="PK818_04095"
     CDS             2399..3550
                     /locus_tag="PK818_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY81949.1"
                     /translation="MAQPARLAPATEPWKDPAARPYVRIEGVTKTFGKVHACDDISLD
                     VYRGEFFALLGGSGSGKSTLLRILAGFEAPDSGRVLIDGVDVTALPPYRRPVNMMFQS
                     YALFPHMSVAQNIGFGLKQDGMPAGAIRDRVQQMLELVQMPQLGNRKPDQLSGGQRQR
                     VALARALAKQPKLLLLDEPLGALDKRLRERTQFELVNIQERVGTTFVMVTHDQEEAMT
                     MSSRIAVMDAGRIVQVSTPAALYEYPSTRFVAEFIGGINLFEGRVLGHDAAAGIVRIA
                     CDALEGGVLQARHPDPLPEGTAVSVAVRPEKVDVHEAAPPRADNVAAGKVRDIAYLGD
                     VSIYHVETASGAEVRVQETHVERSSEPHYDWGDTLWLTWPATAAVVLTA"
     gene            3673..4539
                     /locus_tag="PK818_04100"
     CDS             3673..4539
                     /locus_tag="PK818_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQY81950.1"
                     /translation="MLPMLWLLLFFAVPFLIVLKISFAEAVFGQSPPYSAMIERSADG
                     AASLRLHAGSYALLLQDPLYVAAYLKSLLFAGVSTLCCLLLGYPIAYGIARARPLWRM
                     FLLVLVILPFWTSSLLRTYAMIGMLKANGMLSTTLAAIGLLEPGAAILHTDLAVYIGI
                     VYNYLPFMVLPLTATLMRLDFRLLEAAADLGARPWQGFLRVTLPLSMPGIMAGALLVF
                     IPAVGEFVIPDILGGPDALTIGRVLWTEFFTNRDWPLAAAVAVAMLLLIVIPTLVFEY
                     VENRRERREAHA"
     gene            4536..5342
                     /locus_tag="PK818_04105"
     CDS             4536..5342
                     /locus_tag="PK818_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQY81951.1"
                     /translation="MNRRNWSLWAVMAAGYAFLYVPILSLAVYSFSASRMATVWGGFS
                     TRWYAALFANEQIMQALGRSLAIAALSATLATVLGTASGLALSRFGRFPGRGLLSLMN
                     SAPMVMPDVMLGLSSLLLFVTLQHALGWPERGMGTIMIAHVTLTACYVTVVVRSRMTS
                     LDDSLEEAAMDLGARPWRVFFLITLPLVAPALLAGWLLAFTLSLDDLVIASFTSGPGA
                     STLPMLIYSKVKLGVTPEINALATLIVTVVAAGVAIAGLLMHRRERSLAG"
     gene            complement(5352..7481)
                     /gene="rlmKL"
                     /locus_tag="PK818_04110"
     CDS             complement(5352..7481)
                     /gene="rlmKL"
                     /locus_tag="PK818_04110"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HQY81952.1"
                     /translation="MKFFASCGKGLEYLLADELLALGCTRATAAIAGANAEGELPDAQ
                     RAVLWSRLASRVLWPIAEFECADEHALYAGAAAIDWTRHLDPSHTIAVDAHVSGDAIT
                     HARYAAQRVKDAVVDTLRAQTGSRPDVDVENPDVRLNLVVRKGQATLSIDLGGGSLHR
                     RGWRRAQGEAPLKENLAAAVLLRGGWPQAYAAGGDLLDPMCGSGTLLIEGALIAADVA
                     PGLLRLGDNLPTRWAGFDIGAWKALHIDAIEREARGRASLRPCFTGCDLDPHAIRAAR
                     ENARAAGVDDAIAFAVRDVADLPVQDNPRGVVACNPPYDARLAADPALYRALGDALKR
                     ATPAWSASLLCGDFELARATGLRAQKRYQLFNGPIECSLITCDPIALPAREGEGKREL
                     GEGAQMVANRLRKNLKALRPWREREGVACFRAYDADIPEYACAVDVYTTTGDETWLHV
                     QEYAAPADIPEATTRKRLNELLAAVREVFAVPKQRVAVKTRSTGKGGSKYGNLDHRGE
                     FLTVEEGAAKLQVNLFDYLDTGLFLDHRPLRARMALEARGKRFLNLFCYTGVASVQAA
                     VQGAAQTTSVDLSATYLEWLADNLRGNGVGGTRHRIAQADALKWLEADTGEYDVVFCD
                     PPTFSNSKRADDFDVQRDHVRLLRAALARLAPGGVLYFSNNFRRFRLDEAALAGFAQV
                     EDISASTIPPDYARNPRIHRAWRITPR"
     gene            complement(7478..8008)
                     /locus_tag="PK818_04115"
     CDS             complement(7478..8008)
                     /locus_tag="PK818_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007966252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81953.1"
                     /translation="MKGHCILSHGFESGPDATKVTALADAAERLGWTHERPDYTDLDA
                     KREVSKFGDVLARLQRLHQRAHDASGRGPVVLAGSSLGAWISARVSLEVPVRGLFLMA
                     PPIHMGPAPTLDAAAVPISIIHGWHDELIPAAQVVDWAAPRSARLLLVDDSHRLSGHV
                     QASAEAFAALLADLAA"
     gene            complement(8005..8499)
                     /locus_tag="PK818_04120"
     CDS             complement(8005..8499)
                     /locus_tag="PK818_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014089837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HQY81954.1"
                     /translation="MRQPPQDSELGRLFGQFPRAGRVQWIGLRPARDVPMQPVDVVLA
                     EAGRGLAGDRYAGGSGKRGVTLLQAEHLPAIASLSGHADLAPATLRRNLLVSGIPLAA
                     LQGRRFRIGEVLLEGTGECDPCARMEAALGAGGYNAMRGMGGLCARIIEGGVIRTGDA
                     VVVE"
     gene            8522..9079
                     /locus_tag="PK818_04125"
     CDS             8522..9079
                     /locus_tag="PK818_04125"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQY81955.1"
                     /translation="MPRPPIQRNPLPYAEALAARDLAAIDLVVIHCTELPDLAMARRY
                     GERVLYESGTGNSGHYYIDRDGSIHEYVDPDRIAHHTRGYNPRSIGIELVNSGRYPHW
                     LDSRQQAMDEAYTGAQVEALIALLGDLRARIPSLTSIAGHEDLDLDEVEASDDPALKV
                     RRKRDPGTQFPWQQILDAVPLLRLS"
     gene            9143..10048
                     /locus_tag="PK818_04130"
     CDS             9143..10048
                     /locus_tag="PK818_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010365789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HQY81956.1"
                     /translation="MNSLASELIELLSLERLEDNLFRGQSRDIGTKYVFGGQVLGQAL
                     SAAQATMDSTGAQRDAHSLHGYFLRAGDIEAPIVYSVDRTRDGGSFSVRRVTAIQHGQ
                     PIFFLAASFQQHEDGAEHQLSMPEVPMPEDLVEAPALPPEQLAKLPGKVQRWLARRGP
                     FEFRHVYPRDELNPPKRPPYQQVWFRLSEAVGDAPELHRALLAYASDFHLLGTTTFPH
                     GISYYQPNVLMASLDHALWFHRAFRADDWLLYSIDSPSAQGARGLARGQVFDRDGRLV
                     ASTAQEGLIRVVPAAKADTPLHPKG"
     gene            10050..10865
                     /locus_tag="PK818_04135"
     CDS             10050..10865
                     /locus_tag="PK818_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81957.1"
                     /translation="MRKVFTSPRLENVEAVAALLQDAGIQVKVSEARSYRGSRRSSFS
                     YRESEDAGPQPAVWIIHAEDQPRGRQLLREAGLLDSSRAGESSYVPLSVLERDKDAQA
                     AGKRSRAMRIKLGLLAAILVVIGLMVLTTRKPDAPATVAAAAPPVENPMPTIVPQSTE
                     DLDSYRVDVPSALAKLLIEEKLAARAPEQACIAIDGKDPSPQFMQALQPVAGTAVFVA
                     SRCPGEGAWDIDVHDYMTDGSGSGSVHLMLDLDDAQLLEVEREGERWRVLRER"
     gene            complement(10921..11520)
                     /locus_tag="PK818_04140"
     CDS             complement(10921..11520)
                     /locus_tag="PK818_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HQY81958.1"
                     /translation="MKHFKLLAIAVAAALASAAAFAQTATNAPAAGGQHAQLDANKDG
                     LIDRAEAAKAPRLAAQFDQLDKNKDGKLDAGERPQMAGKRHQGGKQGGHGRMIAADTD
                     KDGRISRAEADAANAKASERFEQMDVNKDGYLDRSDREARSAQRRAECFGKADADKNG
                     QLSRAEFDRMGEACGPMRDGIGKRQPRNNPNPGPFPPRQ"
     gene            11639..12358
                     /locus_tag="PK818_04145"
     CDS             11639..12358
                     /locus_tag="PK818_04145"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HQY81959.1"
                     /translation="MGTDADNVRMFRTGAHACGYWAGREARDLVLDPRDPRLPQIYPL
                     ALEWGFRRSGDIVYRPSCPGCQACVAVRVPVASFTPDRSQRRCLRRNADIDARIVAPQ
                     RTDEHLALYRRYLVARHPGGGMDDHGASEFDQFLVGSWNDGRFLELREHATHRLLALA
                     VTDLAAGALSAVYTFYDPDHGGRGLGTLAVLKQLEWAARDGRQYLYLGYWIAGHAKMD
                     YKRRFQPLEGFNGRGWARLQA"
     gene            complement(12359..12727)
                     /locus_tag="PK818_04150"
     CDS             complement(12359..12727)
                     /locus_tag="PK818_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81960.1"
                     /translation="MNESPGFAVFFFPQALDALGEAIKPYLQDGPAGLHVPCREVDTG
                     GAFIEMTLEGRTADGREVALELMVPGNMVRMIVSARSDGDFGFYDRSKPARALLEPGL
                     AQAAAGNPAVVAANEGGTVA"
     gene            complement(12741..13946)
                     /gene="purT"
                     /locus_tag="PK818_04155"
     CDS             complement(12741..13946)
                     /gene="purT"
                     /locus_tag="PK818_04155"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015472403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HQY81961.1"
                     /translation="MTTIGTPLSPHALRVLLLGSGELGKEVALELQRFGVEVIAADRY
                     ANAPAMQVAHRSHVLDMLDGDAIRALVELERPHLVVPEIEAISTQTLVELEAGQGQRA
                     DPIRIIPTARAARLTMDREGIRRLAAETLGLPTSPYRFVDTFGDYRAAVAALGLPCVV
                     KPVMSSSGHGQSLVRAEADIDAAWDCAQTGGRAGAGRVIVEGFIDFDYEITLLTVRHA
                     GGTSFCEPIGHLQQDGDYRESWQPQPMSALALRRSQDIARAITDDLGGSGVFGVELFV
                     KGDEVWFSEVSPRPHDTGLVTLASQDLSQFALHARAILGLPIPVIRQLGASASCAVLA
                     HGHGVPVFDNIDAALAQPDTQLRLFGKPRVAGHRRVAVTLARAADTDAARAVARDAAA
                     ALTLELRRE"
     gene            14036..14947
                     /locus_tag="PK818_04160"
     CDS             14036..14947
                     /locus_tag="PK818_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81962.1"
                     /translation="MPIHPRQRLSALAALLLCATACAPPPLPFARLAGLITSGDLDEV
                     SGMAASRTHDDVLWAIEDSGNRARLHALTRRGRILARYRVEGARNTDWEDLASFELDG
                     RQYLLVADTGDNGGQRRDIVLHVFEEPRTLENGSLRPAWSIRARWADGPRDCEAVAVD
                     AAAGKVLLVSKKRKPPELFTLPLADPRGQWREARRIGRLAGVPEADEALQRSDPKLAK
                     LSPQVTAADLSPDGRTLAVLTYGSVLFYRREAGEDWGEAVARVPEAHDVPLIPQAEAL
                     AWSKGGAGLYASGEFRPAPIFFLSPDR"
     gene            15044..>15329
                     /locus_tag="PK818_04165"
     CDS             15044..>15329
                     /locus_tag="PK818_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQY81963.1"
                     /translation="MKETPISSLPGIPMLLALLAAIAAGAWIAITGGLQGGSVAQLAA
                     GLLLAFASLFCLFGLYMVEPNQSAVISLFGKYVGTVKENGLRWNNPFFSKK"
BASE COUNT         2263 a         5429 c         5335 g         2302 t
ORIGIN      
        1 gcatgctggc ggcgttgctg tgcgaggtgg tgctgaccgc gatgttcctg gtcgtgatcc
       61 tcggcgcaac ccacaagaac gccgcgcccg gtttcgcgcc gatcgcgatc ggcctgggcc
      121 tgaccctgat ccacctgatc agcatcccgg tcaccaatac ctcggtgaac ccggcgcgtt
      181 ccaccggcgt ggcgctgatg gtcggtggca ccccgctgtc gcagctgtgg ctgttctggg
      241 tcgcgccgat cgtcggcggc ctgctcggcg gcgtggtcta caagtggctg ggcagggact
      301 gacgcccccg acccacgcgt gatggcacgc acggggcccg gcgaatgccg ggcctcgctg
      361 cttgcgcggt acctcagaac tcccaggtga gttgcagcga gacccgccca tcggcctggc
      421 tggcgtcgtc caggccttcg ctcaatgcct cgccgtagct ggaggtgtcg gtgtacagca
      481 ggtcggcgcg cagcggcccg aagctgcggc caagaccgac ctggaagtcg ttgtagctgt
      541 cgccgaccgc atcgttgagg tcgtagtgcc cgacctgggc cttgaggtcg aacccgcttt
      601 cgcccaacgg cagctcgacc ccggcattgc cgtagatgcc atgcccgccg aggttgaaga
      661 tgtccggcga atacgcaagg ccgacatgga agtggtcggc gaaggtcagc acgccctcca
      721 gctcggtgta cgcgtagtcg taaccgtcga ggtggcccgg ataggacacg taggtcaggg
      781 acacgtcgag ctccgcgttt tcgccaagcg caccggacca gccgatgtag ggatcgagtt
      841 cgatgtcgat gccgtcgtcg tcctcgccgg cggcggtgaa gtccacgttc gaggcccacg
      901 cgccggcgta gaagccgctg gcgtgggtca cgctgacgtc cagctgcagg gccgggttgc
      961 cctgggtctg ggacaccccg cgccagacgt aatcgctggt ggcgacgacg ctgccggcga
     1021 cgtcccatgt ttccgccggc tcggcatccg ccggcgcctg ctgggcgagc acggtggtcg
     1081 gcagcaggct ggccagggcc agggcgagca gggacggaag caggcgcatg cgggactccg
     1141 ggaagcgggg attcggcgcg tacgatacaa ccccgccgcc ccggctgcgc ggcgggccgc
     1201 cggcgtacca ttgccgctcg accccagcct ggagcccgcc gtgaccctgc gcctcgcccc
     1261 gctcgcctgc gccatcgccc tgctcgccgc ctgcggcggc aagcccgccg ccgaacccgc
     1321 caccgcgggc actgccgcgc aggcacctga agaaaaggtg ctgaacgtct acaactggtc
     1381 cgactacgtg gccgaagaca ccatcgccaa cttcgagaag gcgaccggga tcaaggtcaa
     1441 ctacgacgtc tattccgaga acgagacgct ggaaaccaag ctgaccgccg gcagcagcgg
     1501 ctacgacgtg gtgttcccgt cggcgcgccc gttcgcgcag cggcaggtga agggcggcct
     1561 gtacctggcg ctggacaagg ccaggctgcc gaacctgtcg cacctcgacc cggcgatcat
     1621 gcagggcctg caggacgtcg accccgccaa tgcgcacgtg gtgccgtaca tgtggggtac
     1681 caccgggctc gggatcaacg tcgacaaggt gaaggccgcg ctcggcgacg gcgcgccgat
     1741 cgactcctgg aacctgctgt tcgacccggc caacgcggcc aggctggcga agtgcgggat
     1801 cagcgtgctg gacgacgacc aggaggcgtt cggcgccgcg ctgatctgga agggcaagga
     1861 tccgaacgct ggcgccgccg acgagatcga gacggtcaag caggtgtacg cggcgatccg
     1921 cccgcacatc cgcaccttca acaacgccga gtacaaggac gcgctggcca acggcgacgc
     1981 ctgcgtggtg atgggctact ccggcgacat cctcgccgcc gccgatgccg ccagcgaagc
     2041 cgccgaggcc gccggcaagc cggcgcccga catccgctac gtgatcccga aggaaggcgc
     2101 gctgcgctgg gtggacgtga tcgcgatccc gaaggacgcc aggcacccgg gcaacgcgct
     2161 ggccttcatc gactacctgc tgcaaccggc ggtgaccgcc gcgatcaccg atgcggtggg
     2221 ctacgccaac ccgaacagcg acgcccgcgc gctggtcgat cccgggatcg ccggcaaccc
     2281 cggcatctac ccggcggccg aggtccaggc ccggctggtc gacccgaagt cgctgccgga
     2341 agcggtgcag cgccagcgcg tgcgcgcctg gaccgcgatc aagagcggtc gctgagcgat
     2401 ggcgcaaccg gcgcggctgg cacccgcaac cgaaccctgg aaggatccgg cggcgcggcc
     2461 atacgtgcgg atcgagggcg tcaccaagac cttcggcaag gtccacgcct gcgatgacat
     2521 cagcctcgac gtctatcgcg gcgaattctt cgccctgctc ggcggctccg gttccggcaa
     2581 gagcacgctg ttgcggatcc tcgccgggtt cgaggcgccc gacagcgggc gcgtgctgat
     2641 cgacggcgtc gacgtcaccg cgctgccgcc gtaccggcgc ccggtcaaca tgatgttcca
     2701 gagctatgcg ctgttcccgc acatgagcgt ggcgcagaac atcgggttcg gcctgaagca
     2761 ggacggcatg cccgcgggcg cgatccgcga ccgcgtgcag cagatgctgg agctggtgca
     2821 gatgccgcag ctgggcaacc gcaagccgga ccagctctcc ggcggccagc gccagcgggt
     2881 ggcgctggca cgcgcgctgg ccaagcagcc caagctgttg ctgctggacg agccgctggg
     2941 cgcgctggac aagcgcctgc gcgaacgcac ccagttcgag ctggtcaaca tccaggagcg
     3001 cgtgggcacc accttcgtga tggtcaccca cgaccaggaa gaagcaatga ccatgtccag
     3061 ccggatcgcg gtgatggacg ccggccgaat cgtgcaggtg tccaccccgg ccgcgctgta
     3121 cgaatatccg tcgacccgct tcgtcgccga attcatcggc ggcatcaacc tgttcgaagg
     3181 gcgcgtgctc ggccacgacg ccgccgccgg catcgtgcgg atcgcctgcg atgcgctgga
     3241 aggcggtgtg ctgcaggccc ggcacccgga cccgctgccg gaaggcaccg cggtcagcgt
     3301 ggcggtgcgg ccggagaagg tcgacgtgca cgaggccgcg ccgccgcgcg cggacaacgt
     3361 ggccgccggc aaggtgcgcg acatcgccta cctcggcgac gtctcgatct accacgtgga
     3421 aaccgcgtcc ggcgccgagg tgcgggtaca ggaaacccat gtcgagcgca gctcggaacc
     3481 gcactacgac tggggcgaca ccctctggct gacctggccg gcgaccgccg cggtggtgct
     3541 caccgcatga gcgcggcacg tccaggcaat ggcatctggt cgttctgggc ggaattcgtc
     3601 cacaacctgt tcggctggtt ccgcgcggtg ggcaccgagt tccgcacccg ccgcgggcgg
     3661 ttcctggtga ccatgctgcc gatgctgtgg ctgctgctgt tcttcgcggt gccgttcctg
     3721 atcgtgctga agatcagctt cgccgaggcg gtgttcgggc agtcgccgcc ctactccgcg
     3781 atgatcgagc gcagcgccga tggcgccgcc agcctcaggc tgcatgccgg cagctacgcg
     3841 ctgctgctgc aggacccgct gtacgtggcc gcgtacctga agtcgctgct gttcgcgggt
     3901 gtttccacgt tgtgctgcct gctgctcggt tacccgatcg cctacggcat cgcgcgcgcg
     3961 cggccgctgt ggcggatgtt cctgctggtg ctggtgatcc tgccgttctg gaccagctcg
     4021 ctgctgcgca cctacgcgat gatcggcatg ctcaaggcca atggcatgct ctcgaccacg
     4081 ctggcggcga tcggcctgct ggagccgggc gcggcgatcc tgcataccga cctcgcggtg
     4141 tacatcggca tcgtctacaa ctacctgccg ttcatggtgc tgccgctcac cgcgaccctg
     4201 atgcggctcg acttccgcct gctggaggcg gccgcggacc tcggtgcgcg gccctggcag
     4261 ggtttcctgc gggtgaccct gccgctgtcg atgcccggga tcatggccgg cgcgctgctg
     4321 gtgttcatcc ccgcggtcgg cgaattcgtg attcccgaca tcctcggcgg cccggatgcg
     4381 ctgaccatcg gccgcgtgct gtggaccgag ttcttcacca accgcgactg gccactggcg
     4441 gcggcggtgg cggtggcgat gctgctgctg atcgtgatcc cgaccctggt cttcgaatac
     4501 gtcgagaacc gccgcgaacg ccgcgaggcg cacgcgtgaa ccggcgcaac tggagcctgt
     4561 gggcggtgat ggcggcgggc tacgccttcc tgtacgtgcc gatcctgtcg ctcgccgtgt
     4621 attcgttcag cgcatcgcgg atggccacgg tgtggggcgg cttctcgacc aggtggtacg
     4681 ccgcgctgtt cgccaacgag cagatcatgc aggcgctggg ccgcagcctg gccatcgccg
     4741 cgctgagcgc gacccttgcc accgtgctcg gcaccgccag cgggctggcg ctgtcgcgct
     4801 tcggcaggtt cccggggcgc ggcctgctga gcctgatgaa ttcggcgccg atggtgatgc
     4861 ccgacgtgat gctcgggctg tcctcgctgc tgctgttcgt caccctgcag catgcgctgg
     4921 gctggccgga gcgcggcatg ggcacgatca tgatcgccca cgtcaccctg accgcctgct
     4981 acgtgacggt ggtggtgcgc tcgcggatga catcgctcga cgacagcctg gaggaagcgg
     5041 cgatggacct cggcgcgcgc ccgtggcggg tgttcttcct gatcaccctg ccgctggtgg
     5101 cgccggcgtt gctggccggc tggctgctgg cgttcaccct gtcgctggac gacctggtga
     5161 tcgccagctt cacctcgggt cccggcgcca gcaccctgcc gatgctgatc tattccaagg
     5221 tcaagctggg ggtgaccccg gagatcaacg cgctggcgac cctgatcgtc accgtggtgg
     5281 cggccggggt ggcgatcgcc gggctgctga tgcaccgccg cgagcgcagc ctggccggct
     5341 aggccgcgcc ctcagcgcgg ggtgatccgc catgcgcggt ggatccgcgg gttgcgcgcg
     5401 taatccggcg ggatggtcga cgcgctgatg tcctcgacct gcgcgaaccc ggccagcgcg
     5461 gcctcgtcca gcctgaagcg gcggaaattg ttggagaagt acagcacgcc gcccggggcc
     5521 agccgcgcca gcgcggcgcg cagcaggcgc acgtggtcgc gctgcacgtc gaagtcgtcg
     5581 gcgcgcttgg agttggagaa ggtcggcggg tcgcagaaca ccacgtcgta ctcgccggtg
     5641 tcggcctcca gccacttcag cgcatcggcc tgggcgatgc ggtggcgggt gccgccgacg
     5701 ccgttgccgc gcaggttgtc ggccagccat tccaggtagg tcgcggagag gtcgacgctg
     5761 gtggtctgcg cggcaccctg caccgccgcc tgcacgctgg ccacgccggt gtagcagaac
     5821 aggttgagga agcgcttgcc gcgcgcctcc agcgccatcc gcgcgcgcag cgggcgatgg
     5881 tcgaggaaca ggccggtgtc gaggtaatcg aacaggttga cctgcagttt cgccgcgccc
     5941 tcctccaccg tgaggaattc gccgcgatgg tcgaggttgc cgtacttgct gccgcccttg
     6001 ccggtgctgc gggtcttcac cgccacccgc tgcttcggca ccgcgaacac ctcgcgcacc
     6061 gccgccagca gctcgttgag gcgcttgcgg gtggtggctt cgggaatgtc cgcgggcgcc
     6121 gcgtattcct gcacgtgcag ccaggtctca tcgccggtgg tggtgtacac gtccaccgcg
     6181 caggcgtatt cgggaatgtc ggcgtcgtag gcgcggaagc aggccacgcc ttcgcgctcg
     6241 cgccacgggc gcagcgcctt caggttcttg cgcaggcggt tggcgaccat ttgcgcgcct
     6301 tcgcccagtt cgcgcttgcc ctcgccctcg cgtgccggca gcgcgatcgg atcgcaggtg
     6361 atgaggctgc actcgatcgg tccgttgaac agctggtaac gcttctgcgc gcgcaggccg
     6421 gtggcgcggg ccagttcgaa atccccgcac agcaggctgg cggaccaggc cggggttgcc
     6481 cgcttcagcg catcgcccag cgcgcgatac agcgccggat ccgcagcaag ccgggcgtcg
     6541 tagggcgggt tgcaggcgac cacgccgcgc gggttgtcct gcaccggcag gtccgcgaca
     6601 tcgcgcacgg cgaatgcaat cgcatcgtcc acgcccgcgg cccgtgcgtt ctcgcgcgcg
     6661 gcgcggatcg catgcgggtc gaggtcgcag ccggtgaaac aggggcgcaa cgaggcacgc
     6721 ccgcgcgcct cgcgttcgat cgcatcgatg tgcaaggcct tccatgcgcc gatgtcgaac
     6781 ccggcccacc gcgtcggcag gttgtcgccc agccgcagca ggcccggcgc cacgtcggcg
     6841 gcgatcagcg cgccctcgat cagcagggtg ccgctgccgc acatcggatc cagcaggtca
     6901 ccgccggcgg cataggcctg cggccagccg ccgcgcagca gcaccgccgc ggccaggttc
     6961 tccttcagcg gcgcctcgcc ctgcgcgcgc cgccagccac gccggtgcag cgaaccgccg
     7021 ccgaggtcga tcgacagcgt ggcctggccc ttgcggacca cgaggttgag ccggacgtcg
     7081 gggttctcga cgtccacgtc cgggcgcgat ccggtctgcg cgcgcagggt gtcgaccacc
     7141 gcgtccttga cccgctgcgc cgcgtaacgc gcatgcgtga tcgcatcgcc ggacacgtgc
     7201 gcgtcgacgg cgatggtgtg cgacggatcg aggtgccggg tccagtcgat ggccgccgcg
     7261 ccggcgtaca gcgcgtgctc gtcggcgcac tcgaactcgg cgatcggcca cagcacgcgg
     7321 ctggccagcc gcgaccacag caccgcgcgc tgcgcgtccg gcaattcgcc ttcggcattc
     7381 gcgccggcga tcgccgcggt tgcgcgggtg cagccgagcg cgagcagttc gtcggccagc
     7441 aggtattcca ggcccttgcc gcaacttgcg aagaacttca tgcggcgaga tccgccagca
     7501 aggcggcgaa ggcctctgca ctggcctgca cgtggcctga gagccggtgc gaatcgtcga
     7561 ccagcagcag gcgcgcactg cgcggcgcgg cccagtccac cacttgcgcg gcgggaatca
     7621 gttcatcgtg ccagccgtgg atgatcgaaa tcggcaccgc ggcggcatcc agcgtgggcg
     7681 ccgggcccat gtggatgggc ggcgccatca ggaacaggcc gcgcaccggc acctcaagcg
     7741 agacccgcgc tgaaatccag gcgcccaggc tggaacccgc gagcacgacc gggccacggc
     7801 ccgacgcgtc gtgcgcgcgc tgatgcagtc gctgcagccg cgccagcacg tcgccgaact
     7861 tgctgacctc gcgcttggcg tcgaggtcgg tgtagtccgg gcgttcgtgg gtccagccca
     7921 ggcgttcggc agcgtccgcg agcgccgtga ccttggtcgc gtccggcccg ctctcgaagc
     7981 cgtgcgagag gatgcaatgg cccttcattc gaccaccaca gcgtcgccgg tgcggatcac
     8041 gccgccttcg atgatccgcg cgcacaggcc gcccatgccg cgcatcgcgt tgtagccgcc
     8101 cgcgcccaac gcggcctcca tgcgcgcgca cggatcgcac tcgccggtgc cttccagcag
     8161 cacctcgccg atccggaaac gacggccctg cagcgctgcc agcgggatgc cggacaccag
     8221 caggttgcgg cgcagcgtgg ccggcgcgag gtcggcatgc cccgaaagcg acgcgatcgc
     8281 cggcaggtgc tcggcttgca gcagggtgac cccgcgcttg ccgctgccgc cggcgtagcg
     8341 gtcgccggcc agtccgcggc cggcttcggc aaggaccacg tcgaccggct gcatgggcac
     8401 gtcgcgggcc ggtcgcaggc cgatccactg cacgcgaccg gcgcgcggga actgcccgaa
     8461 caggcggccg agttcggaat cttggggagg ctggcgcatg cgcgaatgct agcatcgcgg
     8521 catgccccgg cctccgatcc agcgcaatcc gctgccctat gccgaagccc tggcggcgcg
     8581 cgacctggcc gcaatcgacc tggtggtgat ccactgcacc gagctgccgg acctcgccat
     8641 ggcgcgccgc tacggcgagc gcgtgctgta cgaaagcggc accggcaaca gcgggcacta
     8701 ctacatcgac cgcgacggca gcatccacga gtacgtcgat cccgaccgca tcgcccacca
     8761 cacgcgcggc tacaacccgc gcagcatcgg catcgaactg gtcaacagcg gccgctaccc
     8821 gcactggctg gattcccgcc agcaggcgat ggacgaggcc tacaccggcg cccaggtcga
     8881 agccctgatc gcattgctgg gcgatttgcg cgcgcgcatc ccgtcgctca cctcgatcgc
     8941 cgggcacgaa gacctggacc tggacgaagt cgaggccagc gacgacccgg cgctgaaggt
     9001 gcgccgcaag cgcgatcccg gaacgcagtt cccatggcag cagattctcg acgccgtgcc
     9061 cttgctgcgg ctgagttgat gcttccagga cgcccggcgg caggcgcggt catgcgagca
     9121 gcctcccgcc tataattcgc ccatgaattc cctcgcctcc gaactgatcg aactgctctc
     9181 gctggagcgg ctcgaggaca acctgttccg cggccagagc cgcgacatcg gcaccaagta
     9241 cgtgttcggc ggccaggtgc tgggccaggc gctctcggcc gcgcaggcga ccatggattc
     9301 gaccggcgcg cagcgcgacg cgcactcgct gcacggctat ttcctgcgcg ccggcgacat
     9361 cgaggcgccg atcgtctaca gcgtcgaccg cacccgcgat ggcggcagct tctcggtgcg
     9421 ccgggtcacc gcgatccagc acggccagcc gatcttcttc ctggccgcct cattccagca
     9481 gcatgaagac ggcgccgagc accagttgtc gatgcccgaa gtaccgatgc ccgaagacct
     9541 ggtcgaagca ccggcactgc cacccgagca actggcgaaa ctgccgggca aggtgcagcg
     9601 ctggctggcc cggcgcggcc cgttcgagtt ccgccacgtc tatccgcgcg acgagctgaa
     9661 tccccccaag cgcccgcctt accagcaggt gtggttccgc ctgtccgaag cggtcggcga
     9721 cgcgccggaa ctgcaccgcg ccctgctcgc ctacgccagc gacttccacc tgctcggcac
     9781 gaccaccttc ccgcacggca tcagctatta ccagccgaac gtgctgatgg cctcgctgga
     9841 ccacgcgctg tggttccacc gcgcgttccg cgccgacgac tggttgctgt attcgatcga
     9901 cagccccagc gcgcaggggg cgcgcggact ggcgcgcggg caggtgttcg accgcgacgg
     9961 ccggctggtc gccagcaccg cgcaggaagg cctgatccgg gtggtgccgg cggcgaaggc
    10021 cgacaccccg ctgcacccga agggttgaga tgaggaaagt cttcaccagc ccgcgcctgg
    10081 agaacgtcga agccgttgct gcattgctgc aggacgccgg tatccaggtc aaggtctccg
    10141 aggcgcgctc ctaccgcggc agccgccgca gcagcttcag ctatcgcgag agcgaggacg
    10201 ccggcccgca gccggcggtc tggatcatcc acgccgaaga ccagccgcgc ggccggcaac
    10261 tgctgcgcga ggccggcctg ctcgacagct cgcgcgcagg cgagagcagc tacgtgccgc
    10321 tgagcgtgct ggagcgcgac aaggacgcgc aggcggcggg caagcgcagc cgcgcgatgc
    10381 ggatcaagct cggcctgctg gcggcgatcc tggtggtgat cggcctgatg gtcttgacga
    10441 cgcgcaagcc ggacgcgccg gccacggtcg ccgccgcagc gccgccggtg gagaacccga
    10501 tgcccaccat cgtcccgcaa tcgaccgagg acctcgacag ctaccgcgtc gacgtaccct
    10561 cggcactggc caagctgctc atcgaggaga agctcgcggc gcgcgcaccg gagcaggcgt
    10621 gcatcgcgat cgatggcaag gatccatcgc cgcagttcat gcaggcgctg cagccggtgg
    10681 caggtaccgc cgtgttcgtt gcctcgcgat gcccgggcga aggcgcctgg gacatcgacg
    10741 tccacgacta catgaccgac ggctcaggca gcggctcggt ccacctgatg ctggacctgg
    10801 acgacgcgca gctgctcgag gtggagcgcg aaggcgaacg ctggcgcgtg ctgcgcgaac
    10861 gctgaggcag gaagacggcc cgcatcgccg cgggccgctg ccatcgggcg gggtgcgcga
    10921 ctactgcctg ggcgggaacg gacccgggtt cgggttgttg cgcggctgcc gcttgccgat
    10981 gccatcgcgc atcggtccgc aggcttcgcc catccggtcg aactcggcgc ggctgagctg
    11041 gccgttcttg tcggcgtcgg ccttgccgaa gcattcggcg cggcgctgcg cgctgcgcgc
    11101 ctcgcggtcg ctgcggtcga ggtagccgtc cttgttgacg tccatctgct cgaagcgctc
    11161 gctggccttc gcgttcgcgg catccgcttc ggcgcggctg atgcggccgt ccttgtcggt
    11221 gtccgcggcg atcatccggc catgcccgcc ctgcttgccg ccctggtggc gcttgccagc
    11281 catctgcggg cgctcgccgg cgtcgagctt gccgtccttg ttcttgtcga gctggtcgaa
    11341 ctgcgccgcc agccgcggcg ccttggcggc ttcggcgcgg tcgatcagcc cgtccttgtt
    11401 ggcgtcaagc tgggcatgct ggccgccggc ggccggcgca ttggtggctg tctgcgcgaa
    11461 cgcggcggcg gatgccagtg ccgccgcaac cgcgatggcc aggagcttga agtgcttcat
    11521 gtcgattcct cgggtggacc ggcagctgcc gggattgaca tggaaaacgc cgggccgcgc
    11581 aggcggttga cagccaaacc ggcgcgcacc tgaacgatgg cggcggtatg ctcgatggat
    11641 gggcaccgac gccgacaacg tgcgcatgtt ccgcaccggc gcgcacgcat gcggctactg
    11701 ggccggacgc gaagcgcgcg acctggtgct ggacccacgc gacccgcgcc tgccgcagat
    11761 ctatccactt gcgctggagt ggggcttccg ccgctccggc gacatcgtct accggccgag
    11821 ctgccccggc tgccaggcct gcgtggcggt gcgggtgccg gtggcgagct tcacccccga
    11881 ccgcagccag cgccgctgcc tgcggcgcaa cgcggacatc gatgcacgga tcgtcgcgcc
    11941 gcagcgcacc gacgaacacc tcgccctcta tcgccgctac ctggtggcac gccaccccgg
    12001 tggcggcatg gacgatcacg gcgccagcga attcgaccag ttcctggtcg gcagctggaa
    12061 cgacgggcgc ttcctggaac tgcgcgagca cgccacccac cgcctgctgg cgttggcggt
    12121 gaccgacctg gccgcaggcg cgctgtccgc ggtgtacacg ttctacgacc cggaccacgg
    12181 cgggcgcggg ctcggcacct tggcggtgct gaagcaactg gaatgggccg cccgcgatgg
    12241 ccgccagtac ctgtacctgg gctactggat cgccggccac gccaagatgg actacaagcg
    12301 ccgcttccag ccgctggaag gcttcaacgg gcgcggctgg gcgcggctgc aggcctgatc
    12361 aggcgaccgt cccgccttca ttggcggcaa ccaccgccgg atttcccgcc gccgcctgcg
    12421 ccaaaccggg ttccagcagg gcgcgcgccg gcttgctgcg gtcatagaag ccgaaatcgc
    12481 cgtcgctgcg cgcggacacg atcatccgca ccatgttgcc cggcaccatc aactccagcg
    12541 cgacctcgcg gccgtccgcg gtgcgacctt cgagggtcat ctcgatgaag gcaccgccgg
    12601 tgtcgacctc gcggcacggc acgtgcaatc cggcggggcc gtcctgcagg taaggcttga
    12661 tcgcctcgcc cagcgcatcc agcgcctgcg ggaagaagaa cacggcgaaa cccggggatt
    12721 cgttcatgcc ggcaagtctc tcattcccgc cgcaactcca gcgtcaacgc cgccgccgca
    12781 tcgcgggcga cggcgcgtgc cgcatcggta tcggcggcgc gcgccagcgt caccgcgacc
    12841 cgccggtgcc cggccacccg cggcttgccg aacaggcgca actgggtgtc cggctgcgcc
    12901 agcgcggcat cgatgttgtc gaagaccggc acgccgtgac cgtgcgccag caccgcgcat
    12961 gaagcggacg cgcccagctg gcggatcacc gggatcggca ggccgaggat cgcgcgtgca
    13021 tgcagcgcga actggctgag gtcctgcgag gccagggtga ccaggccggt gtcgtgcggg
    13081 cgcggcgaga cctcgctgaa ccagacctcg tcgcccttga cgaacagctc gacgccgaac
    13141 accccggatc cgccgaggtc gtcggtgatc gcgcgcgcga tgtcctgcga acgccgcaac
    13201 gccagtgccg acatcggctg cggctgccag ctttcgcggt agtcgccgtc ctgctgcagg
    13261 tggccgatcg gctcgcagaa gctggtgccg ccggcgtggc gcacggtcag cagggtgatc
    13321 tcgtaatcga agtcgatgaa gccctccacg atcacccgcc cggcgccggc gcggccaccg
    13381 gtctgcgcgc agtcccacgc ggcgtcgatg tcggcctcgg cgcggaccag cgactggccg
    13441 tggccggagg aagacatcac cggcttgacc acgcacggca ggcccagcgc ggcgaccgcg
    13501 gcgcggtagt ccccgaaggt atcgacgaag cggtacggcg aagtcggcag gcccagtgtc
    13561 tccgcggcca gccggcggat gccctcgcgg tccatggtca ggcgcgcggc gcgcgcggtg
    13621 gggatgatcc tgatcggatc ggcgcgctgc ccctgcccgg cttccagttc gaccagggtc
    13681 tgggtgctga tcgcctcgat ctccggcacg accaggtgcg gccgttcgag ttccaccagc
    13741 gcgcggatcg cgtcgccatc cagcatgtcg agcacgtggc tgcgatgcgc gacctgcatc
    13801 gccggcgcat tggcgtagcg gtcggcggcg atcacctcga cgccgaagcg ctgcagctcc
    13861 agcgccactt ccttgccaag ctcgccggag ccgagcagga gcacgcgcag cgcgtgcggc
    13921 gagagcggcg tgccgatcgt tgtcatgcgg gttccggacg cgttgggagc tgcgattcta
    13981 gcggaggcga ccgccggggc tgtgccgcgg cgcgggtcca tgccatcctc cgcggatgcc
    14041 gatccacccc cgacagcggc tgtccgccct ggccgcgctg ctcctgtgcg cgaccgcgtg
    14101 cgcgccgccg ccgctgccgt tcgcgcggtt ggcggggctg atcaccagcg gcgacctgga
    14161 cgaagtcagc gggatggcgg catcgcgcac ccacgacgac gtgttgtggg cgatcgagga
    14221 cagcggcaac cgggcgcgcc tgcacgcact cacgcgccgc ggccggatcc tcgcccgtta
    14281 tcgggtcgag ggcgcccgca acaccgactg ggaagacctc gccagcttcg aactcgatgg
    14341 caggcagtac ctgctggtgg ccgacaccgg cgacaacggc ggccagcgcc gggacatcgt
    14401 gctgcatgtc ttcgaggagc cgcggacact cgaaaacggc tccctgcggc cggcatggtc
    14461 gatccgcgcg cgctgggccg atggcccgcg cgattgcgag gcggtggcgg tggacgccgc
    14521 ggccggcaag gtgctgctgg tctcgaagaa gcgcaagccg ccggaactgt tcaccctgcc
    14581 gctggccgac ccgcgcggcc agtggcgcga ggcacgccgg atcggccgcc ttgcaggggt
    14641 gccggaggcc gatgaggcgc tgcagcgcag cgatcccaag ctggcgaaac tgtcgccgca
    14701 ggtcaccgcc gccgatctct cccccgatgg ccgcaccctg gccgtactca cctatggcag
    14761 cgtgctgttc taccgccgcg aagccggcga ggactggggc gaagcggtgg cgcgggtgcc
    14821 ggaagcgcac gacgtgccgc tgatcccgca ggccgaggcg ctggcctgga gcaagggcgg
    14881 cgccggcctg tacgccagcg gcgaattccg cccggcaccg atcttcttcc tgagcccgga
    14941 tcgctgaccc ggcccaaccg ttcgtcggat cgtgcttggc agatagatat cattttgata
    15001 tcaatatctc gttcgcccat cccgggccgt tgcgagagcc accatgaaag agaccccgat
    15061 cagttccctt cccggcatcc cgatgctgct ggccttgctg gcggcgatcg cggccggtgc
    15121 ctggatcgcc atcaccggtg gcctgcaggg cggctccgtc gcccagttgg ccgcgggcct
    15181 gctgctggcc ttcgcctcgc tgttctgcct gttcggcctg tacatggtcg agcccaacca
    15241 gtcggcggtg atcagcctgt tcggcaagta cgtcggcacg gtcaaggaaa acggcctgcg
    15301 ctggaacaac ccgttcttca gcaagaaga
//
LOCUS       DAORUK010000039        15318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_3633_length_15318_cov_18.290506, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000039 DAORUK010000000
VERSION     DAORUK010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15318
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3633_length_15318_cov_18.290506"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /gene="hisE"
                     /locus_tag="PK818_04170"
     CDS             <1..234
                     /gene="hisE"
                     /locus_tag="PK818_04170"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HQY81964.1"
                     /translation="ADSDASYTRRLFDAGIARIAQKVGEEGVETALAAVTADDASLRG
                     EAADLTYHLLVLLRARGLDWNAVRAELARRAGA"
     gene            complement(236..730)
                     /gene="phaR"
                     /locus_tag="PK818_04175"
     CDS             complement(236..730)
                     /gene="phaR"
                     /locus_tag="PK818_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HQY81965.1"
                     /translation="MSAIRIIKKYPNRRLYDTEISSYITIEDVRQLIVDGESFEVRDA
                     KTGDDLTRQVLLQIITEHEQDGQPMLSTQLLSQLIRFYGDSLQGFMGNYLERSMQVFL
                     DQQQQFRQQMGGLIGQTPWTMLNQLTERNMELWKDFQKNLVGGIGNPAAPPPPRERGS
                     QKQR"
     gene            985..1773
                     /locus_tag="PK818_04180"
     CDS             985..1773
                     /locus_tag="PK818_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HQY81966.1"
                     /translation="MDKRLKDRFGGIDRLYGSGALERFAGCHVTVVGIGGVGSWAVEA
                     LARSGIGKLTLIDADDLCVSNTNRQLPALEGQYGRAKVEAMGERCRAINPGIDLDLKP
                     QFLTPTNMGELLEAPGDVVLDACDSFRSKVEAIAYCRRRKQPIVAVGSAGGRSDVTQI
                     RVRDLSRTEHDAMLSLIRRKLRGEFNFPKNHDRYFGVPAVYSLENVRYPQADGSVCGV
                     RPQLGKDEGLKLDCGAGLGAATHVTGAFAFAAVGKVLEMLLKQR"
     gene            complement(1844..2665)
                     /locus_tag="PK818_04185"
     CDS             complement(1844..2665)
                     /locus_tag="PK818_04185"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007972083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQY81967.1"
                     /translation="MRLIDSHCHFDAAAFDADRAQAHARALAAGVAAQVVPAVAAAGW
                     PKLKAVCAEFAGAYPAYGLHPMFLASHRQAHLHELRDWIARERPVAVGECGLDHFVQG
                     LDHDAQLHYFDAQLHLAREFDLPVIVHARRAVDAVIAAIRGIGGLRGVVHSFSGSAEQ
                     AAQLHKLGFLLGIGGPVTYERANRLRGLVAAMPLDQLLLETDAPDQPGVDHRGERNEP
                     AHLAKVLEVVAELRGMPLADVAAATTANAELLFGLSGWSRRTAGVTRAARSTRPV"
     gene            complement(2679..2912)
                     /locus_tag="PK818_04190"
     CDS             complement(2679..2912)
                     /locus_tag="PK818_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81968.1"
                     /translation="MANPMLAPLFGFLGRLSYPRLFALTAALFAVDVVIPDFVPFADE
                     LLLGLGTLLLANWKKRKQPADARPPIEGGSSQR"
     gene            3129..4292
                     /locus_tag="PK818_04195"
     CDS             3129..4292
                     /locus_tag="PK818_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY81969.1"
                     /translation="MALNPYDLFDVRSLLSDEERAVQDTVARFTDERVRPIIGDAFDQ
                     GRFPRELVPEIAEMGLLGSSLPAMYGCAGLNGVSYGLICQELERGDSGIRSFVSVQSS
                     LCMYPIYAYGSEEQRQRWLPDMAAGKVIGCFGLTEPHGGSDPANMKTHAKRDGDDWVI
                     NGSKMWITNGNLADIAIVWAQTDDGIQGFLLEKGMPGFTAQEIKHKMSLRASVTSALF
                     FDNVRVPDSSRLPNVKGLKGPLGCLTQARYGITWGPIGAAIACLDEVLDYTKERILFE
                     RPVAATQSAQIKMAEMARRITLAQLLVLQLGRLKDAGTMQPQQVSLAKWNNCRMAIDI
                     ARECRDLLGGAGITTEHAAIRHALNLESVITYEGTETVHQLVIGRELTGISAF"
     gene            4354..4959
                     /locus_tag="PK818_04200"
     CDS             4354..4959
                     /locus_tag="PK818_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HQY81970.1"
                     /translation="MITLYGLSASGNCYKLQLACAQLGIEHAWEEIDVTRGENLSPEF
                     LALNDRGKVPVLRLEDGRTLSESNAILNYLAQGTRLLPDDHWGRAKTLQWMFFEQYSH
                     EPCIAVSRFIRKYLPAQHPRRASLEALQASGNEALAAMERHLARLPWFGGETYTIADI
                     ALFAYTHVADEAGFDLGAYPRVEHWLKRVMQQPGFVPMRND"
     gene            4990..7260
                     /locus_tag="PK818_04205"
     CDS             4990..7260
                     /locus_tag="PK818_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQY81971.1"
                     /translation="MFATVPDPIPARMRGLNRAEICDVNFQEFVRGWQGQAQPKPAPG
                     EAILDGSALDARGFRELFESQLVSRHLDLMARVLRVQNKVFYTIGSSGHEGNAMVARA
                     TRHTDPAFLHYRSGGFMAERFRKLPGMDPIMDSALSFAASAEDPASGGRHKVWGSKPL
                     WVLPQTSTIASHLPKALGTAVAIEAAHRIGHQLPIPDDSIAICSFGDASANHATAQTA
                     FNAASWTAYQKLPAPVLYVCEDNGIGISVKTPTGWIGESFRNRPDLDYFFADGLDLAG
                     GYADVLRAVEHCRRSRRPTFLHLRTTRIMGHAGTDFEIEWRSIEELCAVEAGDPLLRS
                     AAIALESGAMTRDEILALYEDTRKKCFAAAEDADQRPRIETLEHVMRPLAPYTPDQVM
                     AEATRLPSPEARLQAFGSEDKLPEKQVPRHLAIQINQALHDLFAKYPETLLFGEDVAQ
                     KGGVYTVTKGLHKAFGNKRVFNTLLDETMILGMAQGFANMGMLPIPEIQYLAYLHNAI
                     DQIRGEAASLQFFSNDQFRNPMVVRIAGLGYQRGFGGHFHNDNSMTALRDIPGLVVGC
                     ASRGDDAAMMLRTLTALAKVDGRVSVFLEPIALYMTKDLHEPGDGLWLTEYPAPDRAL
                     VLGEERVYNAEATDCVIFSYGNGMPMSLRAARAIEAKHGWKVRVVDLRWLVPLNEAAI
                     ARHARECARILVVDEGRHSAGVGEGVITAITEAGLGGKPGRRVCGADTYTPLAGAAFL
                     VIPKDEDIVAAAGLLA"
     gene            complement(7278..8936)
                     /locus_tag="PK818_04210"
     CDS             complement(7278..8936)
                     /locus_tag="PK818_04210"
                     /inference="COORDINATES: protein motif:HMM:NF005760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HQY81972.1"
                     /translation="MPNGAQALLQTLADAGIEVCFSNPGTSEMHFVAALDSEPRMRAV
                     LALFEGVATGAADGYARMAGKPAATLLHLGCGLGNGLANLHNARKGKVPVVNIVGDHA
                     THHVPLDAQLQSDIETVARNVSPGFVRTSRSTADLCRDAVDAIKAARGLPGQVATLVL
                     PADVSWGEGGVPCPPPPAPAPEPADDACVDAIAHAIRAGGRCALLLGGQALREPGLLA
                     AARIAAQHGAKLFCEVFPTRLERGAGLPPVERIAYLAELASLQLAGFEHLVLVDAKAP
                     VSFFAYPGKQGELVPDGCNVHTLATPAQDAVASLDKLVAALDAARAVPALQAAARPSL
                     PRGRLTAPKVCKAVGALLPERAILIDEAITSGLMLGVMTEGGPHHDLITLTGGAIGQA
                     LPNAVGAAIACPDRPVIALVGDGTAMYTIQALWTLARENLDVTAVIFNNASYSVLNVE
                     LDRVGAERAGPKARSQLDLHGPVLDFARLAQGMGVHAVRADTAADFCKALEYALAHPG
                     PHLIDARVPESLGGAKRRLLPWLLRALPGLPPALARGLKRKIAP"
     gene            complement(8960..10099)
                     /locus_tag="PK818_04215"
     CDS             complement(8960..10099)
                     /locus_tag="PK818_04215"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HQY81973.1"
                     /translation="MPATSDDHRARAGGRLPRFLADYVDGGAGAEQTLAANVAAWPGI
                     GIRQRVLVDVAGVDTASTLAGDACRMPLALAPLGLGGLLARRGELQALRAATAAGVPF
                     TLSTVGICSIEELAAGTGAPFWFQLYWLRDRGLVADLLQRAWAAGCRTLVFTVDLPLP
                     GMRHRDTRNGMAQPGVRPKLLRLTQVLARPGWLWDVALRGKPLLFGNLQRQLPNARDL
                     DAFRAFIDAQFDPGVTWAELDWLRARWPGRLLLKGILDVDDARAAVAVGADGLVVSNH
                     GGRQLDGAAATALRLPAIAAAVGADTEVLVDGGLRSGVDLFRACALGARGALIGRPWA
                     WALAGGGEAGLRGLLATWQNELATTMALAGVTRIAEIGPQHLDRA"
     gene            10475..11446
                     /locus_tag="PK818_04220"
     CDS             10475..11446
                     /locus_tag="PK818_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81974.1"
                     /translation="MFRHAIAGLVLASSALVSLAVPAVADAGEPVVAVHDIGNLKGVK
                     RVAVTSFVVQYVVAQQTTSRGGASSGIDLGKDIDPARLQQATEQIYAQFLADLGASGI
                     EVVAPEALAASKGFREMLGKSPATPLAMSTWSRGKDGGYSSVFHVPKGLPMVVRDDYE
                     YLKGRGLGQVTDPSLSIGGGIKLASTNWRYYDKAVQDELQAATLLVRVFVPLAYNESS
                     TSIAGPWQKDSAKTTAGLRIGERFTRMAVVQDDEIAKIYLGEPLLAGDSIIANQGAKS
                     GFTASKLLFGSKNNESFALDADQYFQDVPAASAKVLNAFIKAMNDNR"
     gene            complement(11498..13105)
                     /locus_tag="PK818_04225"
     CDS             complement(11498..13105)
                     /locus_tag="PK818_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY81975.1"
                     /translation="MTALQSDVLVIGGGLAGIVTALEALRAGQRVTLVDRDTPERFGG
                     LALWAFGGMALVGTPLQASKKIIDSPEIALRDWMRFGEMDPDDRFPMQWARHYVEQSR
                     PQVHDWLQAHGIRFMPAVNWVERGRDGEGNSVPRYHVVWGTSRELTRRMIAALREAGA
                     GGRLRLLHGHRVAALEHAGGRIAGALAIGEATGGEVRLAAPVVVLAMGGINGSHAQVR
                     GNWPQDRPCPASMLNGAHPFADGRLHQIASGLGAQVTHAGEMWNYAAGFPHPFPHFDG
                     HGLSAIPCKSALWLDHTGRRIGPEPLVTGFDTHWLCQRVAAQEKPWTWHLLNWRIAAK
                     EFAISGAEHNPRIRDKQFLRFLKETLLGNHRLVRQMQRESAHFLVADTLAELAAKMNA
                     LTCSHAIDPATLQATADAFDAHFAAGANPQDDAQVRRILHARQWGPDALRTCKPAPLQ
                     QPGAGPYIAIHMQLITRKSLGGLRTDLHSRVLDADEQPIDGLYCVGEAAGFGGGGACG
                     KRSLEGTFLPGCILTARAAARSIATGA"
     gene            complement(13197..14051)
                     /locus_tag="PK818_04230"
     CDS             complement(13197..14051)
                     /locus_tag="PK818_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HQY81976.1"
                     /translation="MSHKPRTAPAQEFRLPGHDVLAIGGRDAVAFAQAQFMNDVAALV
                     DGGWHWNGWLTPKGRVIALFALLRQDAETLWLVLPDAGAGELATQLQRFVFRSKVRLQ
                     PRDDLHANGRFGAPALASGSTIAQADGAFELDLGADGGPRCLRIANAAAEADAALASR
                     WRGFDLQHGLPRLRDAQLEAWTPQHLSLDRLRAYSVKKGCYPGQEIVARTHFLGQVKR
                     GLALFHAGAPLPEGSAISADGRDIGSVACVEEDLALAVMPLDAPAAGLVAAGIDLLPA
                     PLLGGLAR"
     gene            14154..14279
                     /locus_tag="PK818_04235"
     CDS             14154..14279
                     /locus_tag="PK818_04235"
                     /inference="COORDINATES: protein motif:HMM:NF019511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HQY81977.1"
                     /translation="MPRAASEPATAIDPAVEIGGRDGPEPTRYGDWEKNGRCIDF"
     gene            14327..14737
                     /gene="sdhC"
                     /locus_tag="PK818_04240"
     CDS             14327..14737
                     /gene="sdhC"
                     /locus_tag="PK818_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HQY81978.1"
                     /translation="MAAPDTHVRQRPLSPHLQVYRWQVQMASSILHRATGVILSVGAL
                     ALVWGLLALAGGPEPWQAFIACVGSPLGTVALFGFSWALAYHLLNGIRHLVQDAGHGF
                     AIADFVRSSWVSIIGSVLLVAAVWAIVLVRWGQA"
     gene            14734..15126
                     /gene="sdhD"
                     /locus_tag="PK818_04245"
     CDS             14734..15126
                     /gene="sdhD"
                     /locus_tag="PK818_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HQY81979.1"
                     /translation="MNNANDLRTPLKRARGLGSAKDGTGHFTWQRITAIAVALLGIYL
                     LGLLIGIGGADYDRARMIVADPLNATLLVAFVIAAFWHARLGLQVVIEDYVHTPLLAG
                     AAHLASLFVCALAAIGGVLAIVRIALGA"
     gene            15182..>15318
                     /locus_tag="PK818_04250"
     CDS             15182..>15318
                     /locus_tag="PK818_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQY81980.1"
                     /translation="MIVVGAGGAGLRATFGLAEKGLQTACISKVFPTRSHTVAAQGGI
                     SA"
BASE COUNT         2334 a         5316 c         5392 g         2276 t
ORIGIN      
        1 gccgacagcg acgccagcta cacgcggcgc ctgttcgatg cggggattgc gcgcatcgcg
       61 cagaaggtgg gcgaggaggg cgtggaaacc gcgctggcgg cggtcaccgc agacgacgcg
      121 tcgctgcgcg gcgaagccgc cgacctcacg taccacttgc tggtcctgct gcgcgcgcgc
      181 gggctggact ggaacgctgt ccgcgccgaa ctggcgcggc gcgcgggcgc ctgattcagc
      241 gctgtttctg gctgccgcgc tcgcgcggcg gcggtggcgc cgcaggattg ccgatgccac
      301 cgaccaggtt cttctggaag tccttccaca gctccatgtt gcgttcggtg agctggttga
      361 gcatggtcca cggggtctgc ccgatcagcc cgcccatctg ctggcggaac tgctgctgct
      421 ggtccaggaa cacctgcatc gagcgttcca ggtagttgcc catgaagccc tgcagggagt
      481 cgccgtagaa gcggatcagc tggctcagca actgggtgga cagcatcggc tgcccgtcct
      541 gctcgtgctc ggtgatgatc tgcagcagca cctgccgggt caggtcgtcg ccggtcttgg
      601 catcccgcac ttcgaacgac tcgccatcca cgatcagctg gcgcacatcc tcgatggtga
      661 tgtagctgga gatctcggtg tcgtagaggc ggcggttcgg atacttcttg atgatgcgga
      721 tcgcggacat tgagcaaaca ctctagcggc gctgggcgca gcatggcgca gtgcaccacg
      781 ggttgcaact cccgggctcc gcccggaagt tcccgaagtt ttcttcgaaa acgtcgggcc
      841 ccgctacgtg cttccacacc cccatccacg cctgcggcgt gaatcgcccg ctgactcggg
      901 gggctgttcc ggtgctgcag caaacctgca gcatgggtgc agcaaaaccg gttggccggg
      961 acgcgtccgg ataaactggg gcgcatggac aagcgtttga aagaccgatt cggcggcatc
     1021 gaccggctgt atggcagcgg cgcgctggag cggttcgccg ggtgccacgt caccgtggtc
     1081 gggatcggcg gggtgggttc ttgggcggtg gaggcactgg cgcgcagcgg gatcggcaag
     1141 ctcacgctga tcgacgccga cgacctgtgc gtgtcgaata ccaaccgcca gttgcccgcc
     1201 cttgaaggcc agtacgggcg ggccaaggtg gaggcgatgg gcgagcgttg ccgcgcgatc
     1261 aatcccggca tcgatctcga cctgaagccg cagttcctga cgcccacgaa catgggcgag
     1321 ttgctggagg cgcccggcga cgtggtgctg gacgcctgcg acagcttccg cagcaaggtc
     1381 gaggcgatcg cgtattgccg tcgccgcaag cagccgatcg tggcggtggg atcggcaggc
     1441 gggcgcagcg acgtgaccca gatccgcgtg cgcgacctct cgcgcaccga gcacgacgcg
     1501 atgctctcgc tgatccgcag gaaactgcgt ggcgagttca atttccccaa gaaccacgac
     1561 cgctatttcg gggtgccggc ggtgtattcg ctggagaacg tgcgctatcc gcaggccgat
     1621 ggcagcgtct gcggcgtccg cccgcagctg ggcaaggacg agggactgaa gctggactgc
     1681 ggcgccggcc tgggcgcggc aacccacgtc accggcgcgt tcgcgttcgc ggcggtaggc
     1741 aaggtgctgg agatgttgct caagcagcga tgaagtccga ccgggagtgg cgcacggccg
     1801 gggtggcgcg cgagcaggcc atggatggcc tgcgcccgcg agatcacaca ggacgtgtgg
     1861 accgagcggc gcgcgtcacc ccggccgtgc gccgcgacca gccggagagt ccgaacaaca
     1921 gctccgcatt agctgtagtt gccgctgcga catccgcgag tggcatgccg cgcaattccg
     1981 ccacgacttc caggaccttc gccagatgcg caggctcgtt gcgctcgccg cggtggtcca
     2041 cgccaggttg gtcgggcgcg tcggtttcca gcaacagctg atccagcggc atcgccgcca
     2101 ccagcccgcg caagcggttc gcgcgttcgt aggtcaccgg tccgccgatg ccgagcagga
     2161 agccgagctt gtgcagctgc gcggcctgct cggcgctgcc ggagaagctg tggaccacgc
     2221 cacgcaggcc gccgatcccg cggatcgcgg cgatcaccgc atccaccgcg cgcctggcgt
     2281 gcacgatcac cggcaggtcg aattcccggg ccaggtgcag ttgggcatcg aagtagtgga
     2341 gctgggcgtc gtggtccagg ccttggacga agtgatccag cccgcattcg ccgaccgcga
     2401 ccggccgctc gcgcgcgatc cagtcgcgca gctcgtgcag gtgcgcttgc cggtgcgatg
     2461 cgaggaacat cggatgcagg ccataggccg ggtatgcgcc tgcgaactcc gcgcagaccg
     2521 ccttcagctt cggccaaccg gcggcagcga cggcgggcac cacctgcgcc gcgaccccgg
     2581 ccgccagtgc ccgcgcatgc gcctgggcac gatccgcgtc gaacgccgcg gcgtcgaagt
     2641 ggcagtggct gtcgatgagc cgcacgcggg tggctgcgtc agcgttggct ggacccgcct
     2701 tcgatcggcg gcctggcgtc tgcgggttgc ttgcgcttct tccagttcgc cagcagcagg
     2761 gtcccgaggc cgagcagcag ttcgtcggcg aatggcacga agtccggaat caccacgtcg
     2821 accgcgaaca gcgcggcggt gagtgcgaac agccgcggat agctcagtct gccgaggaag
     2881 ccgaacaacg gtgcaagcat cggattggcc atggcatcgg cagcaggggg cgtcgggcga
     2941 acgctagcat gcggtggcaa acctgcgccg gcggccgtgg ctggcggcgg tttcggcggc
     3001 ggctggcgaa atcgcgcggt tttggcgata tgccggcatt ccgccggacc gatccttgcg
     3061 tttcgcaggt accgtctgcg cggcatgcgc cattacaatg gcggatcgcc cgaatcgact
     3121 gcaacgccat ggccctcaat ccctacgacc tgttcgacgt ccgttccctg ctcagcgacg
     3181 aggagcgcgc ggtgcaggac accgtggcgc gtttcaccga cgaacgggtg cgcccgatca
     3241 tcggcgatgc gttcgaccag ggacggttcc cgcgggagct ggtgccggag atcgccgaga
     3301 tgggcctgct gggttcgtcg ctgcctgcga tgtacggctg cgccggcctc aacggcgtca
     3361 gctacggcct gatctgccag gagctggagc gcggcgattc cggcatccgc agcttcgtgt
     3421 ccgtgcagag ctcgctgtgc atgtatccca tctacgccta cggcagcgaa gagcagcgcc
     3481 agcgctggct gccggacatg gccgccggca aggtgatcgg ctgcttcggc ctgaccgaac
     3541 cgcacggcgg ctccgacccc gccaacatga agacccacgc caagcgcgac ggcgatgact
     3601 gggtgatcaa cggctcgaag atgtggatca ccaacggcaa cctggccgac atcgccatcg
     3661 tctgggcgca gaccgacgac ggcatccagg gcttcctgct ggaaaagggc atgcccgggt
     3721 tcaccgcgca ggagatcaag cacaagatgt cgctgcgcgc ctcggtcacc agcgcgctgt
     3781 tcttcgacaa cgtgcgggtg cccgattcca gccgcctgcc caacgtgaag ggcctgaagg
     3841 gcccgctggg ctgcctgacc caggcccgct acggcattac ctggggcccg atcggcgccg
     3901 ccatcgcctg cctggatgaa gtgctggact acaccaagga acggatcctg ttcgagcgcc
     3961 cggtggccgc cacccagagc gcgcagatca agatggccga gatggcccgc cggatcaccc
     4021 tggcgcagtt gctggtgctg cagctgggcc ggctgaagga tgccggcacc atgcagccgc
     4081 agcaggtatc gctggcgaag tggaacaact gccggatggc gatcgacatc gcccgcgaat
     4141 gccgcgacct gctcggcggc gccggcatca ccaccgaaca cgccgcgatc cgccacgccc
     4201 tgaacctcga atccgtcatc acctacgaag gcaccgagac cgtgcaccag ctggtgatcg
     4261 gccgcgagct gaccggcatc agcgcgttct gactcagtgc gcggcggcgg cgatccgcca
     4321 ccgcgcgcag gcgttcgtgc aggagtggca cgcatgatca cgttgtacgg cttgtcggca
     4381 tccggcaact gctacaagct gcaactggcc tgcgcgcagc tgggcatcga gcacgcgtgg
     4441 gaagagatcg acgtcacccg cggcgagaac ctgtcgccgg aattcctggc gctgaacgat
     4501 cgcggcaagg tgccggtgct gcggctcgag gatggccgca ccctgtccga gtcgaatgcg
     4561 atcctgaact acctggcgca gggcacgcgg ctgctgccgg acgaccactg gggccgcgcg
     4621 aagacgctgc agtggatgtt cttcgagcag tacagccacg agccctgcat cgcggtctcg
     4681 cgcttcatcc gcaagtacct gccggcgcaa cacccgcggc gtgcctcgct ggaggccctg
     4741 caggcatccg gcaacgaggc gctggcagcg atggagcgcc acctggcgcg gctgccgtgg
     4801 ttcggcggcg agacctacac catcgccgac atcgccctgt tcgcctacac ccacgtggcc
     4861 gacgaagccg gcttcgacct cggcgcctat ccgcgggtcg agcactggct caagcgcgtc
     4921 atgcagcagc ccggcttcgt gccgatgcgc aacgactgac caattccact tcgaagaccg
     4981 acaccaagca tgttcgccac cgttcccgat ccgatccccg cgcgcatgcg cggcctcaac
     5041 cgcgccgaga tctgcgacgt caacttccag gagttcgtgc gtggctggca gggccaggcg
     5101 cagccgaagc cggcgccggg ggaggcgatc ctcgatggca gtgcgctgga tgcgcgcggc
     5161 ttccgcgagc tgttcgaatc gcagctggtt tcgcgccatc tcgacctgat ggcgcgcgtg
     5221 ctgcgggtgc agaacaaggt cttctacacc atcggcagca gcggccacga aggcaatgcg
     5281 atggtggcgc gcgccacccg ccacaccgac ccggcgttcc tgcactaccg cagcggcggc
     5341 ttcatggcgg aaagattccg caagctgccg gggatggatc cgatcatgga ttcggcgctg
     5401 agcttcgcgg ccagtgccga agacccggcc agcggcggcc gccacaaggt ctggggcagc
     5461 aagccgctgt gggtgctgcc gcagacctcg acgatcgcct cccacctgcc gaaggcgctg
     5521 ggcaccgcgg tcgcgatcga ggccgcgcac cggatcggcc accagttgcc gatccccgat
     5581 gattcgatcg cgatctgctc gttcggcgac gcctccgcca accatgccac cgcgcagacc
     5641 gcgttcaacg cggccagctg gaccgcctac cagaagctgc ccgcgccggt gctgtacgtg
     5701 tgcgaggaca acggcatcgg catctcggtg aagacgccga cgggctggat cggcgagagc
     5761 ttccgcaacc gcccggacct cgactacttc ttcgccgacg gcctcgacct ggctggcggc
     5821 tatgcagacg tgctgcgcgc ggtcgagcac tgccgccgca gccgccggcc gaccttcctg
     5881 cacctgcgca ccacccgcat catgggccac gccggcaccg acttcgagat cgagtggcgc
     5941 agcatcgagg agctgtgcgc ggtggaggcc ggtgatccgt tgctgcgcag cgccgccatc
     6001 gcgctggaat ccggggcgat gacccgggac gaaatcctgg cgttgtacga agacacgcgg
     6061 aagaagtgct tcgccgctgc cgaggacgcc gaccagcgtc cgcgcatcga gacgctggaa
     6121 cacgtgatgc gcccgctggc gccgtacacc ccggaccagg tgatggcgga agccacccgc
     6181 ttgccgtccc cggaggcgcg actccaggcc ttcggcagcg aggacaagct gcccgagaag
     6241 caggtgccca ggcaccttgc catccagatc aaccaggccc tgcacgacct gttcgccaag
     6301 tacccggaga cgttgctgtt cggcgaggac gtggcccaga agggcggcgt gtacacggtc
     6361 accaagggcc tgcacaaggc gttcggcaac aagcgcgtgt tcaacacgct gctggacgaa
     6421 accatgatcc tgggcatggc ccagggcttc gccaacatgg gcatgctgcc gatccccgag
     6481 atccagtacc tggcctacct gcacaacgcc atcgaccaga tccgcggcga ggcggcatcg
     6541 ttgcagttct tcagcaacga ccagttccgc aacccgatgg tcgtgcgcat cgccgggctg
     6601 ggctaccagc gcggcttcgg cgggcatttc cacaacgaca actcgatgac cgcgctgcgc
     6661 gacatccccg gcctggtggt ggggtgcgcc agccgcggcg acgatgcggc gatgatgctg
     6721 cgcacgctca ccgcgctggc gaaggtcgat ggccgggtca gcgtgttcct ggagccgatc
     6781 gcgctgtaca tgaccaagga cctgcacgag ccgggcgacg gactgtggct caccgagtac
     6841 ccggcgcccg accgcgcgct ggtgctgggc gaggaacgcg tctacaacgc ggaggccacc
     6901 gactgcgtga tcttcagcta cggcaacggc atgccgatga gcctgcgtgc cgcccgcgcg
     6961 atcgaggcga agcatggctg gaaggtccgg gtggtcgacc tgcgctggct ggtgccgttg
     7021 aacgaggcgg cgatcgcccg ccacgcccgc gagtgcgcgc gcatcctggt ggtcgatgaa
     7081 ggccgccaca gcgcgggcgt cggcgaaggc gtgatcaccg cgatcaccga agctggcctc
     7141 ggcggcaagc ccggtcgccg cgtctgtggc gcggatacct acaccccgct ggccggtgcc
     7201 gcgttcctgg tgatcccgaa ggacgaggac atcgtggccg ccgccggctt gcttgcctga
     7261 gcgcccggcg tcgcgcgcta cggcgcgatc ttgcgcttta gcccgcgcgc cagcgccggc
     7321 ggcagcccgg gcagcgcgcg cagcagccac ggcaacagcc tgcgcttggc gccgccgagg
     7381 gattccggca cccgtgcgtc gatcaggtgc gggccgggat gcgccagcgc gtattccagc
     7441 gccttgcaga aatccgcggc ggtatcggcg cgcaccgcgt gcacgcccat gccctgcgcc
     7501 agccgggcga agtccagcac cggcccgtgc aggtcgagct gggagcgcgc cttcgggccg
     7561 gccctttcgg cgccgacgcg gtccagctcg acattgagga ccgaatagct ggcgttgttg
     7621 aagatgaccg cggtgacgtc gagattctcg cgcgcaagcg tccacagcgc ctggatcgtg
     7681 tacatcgcgg tgccatcgcc gaccagggcg atgaccgggc ggtcggggca ggcgatcgcc
     7741 gcacccaccg cgttgggcag tgcttgcccg atggcgccgc cggtgagggt gatcaggtcg
     7801 tggtgcgggc cgccttcggt catcaccccg agcatcaggc cggaggtgat cgcctcgtcg
     7861 atcaggatcg cgcgctccgg cagcagcgcg ccgaccgcct tgcacacctt gggcgcggtc
     7921 agccggccgc gcggcagcga cgggcgcgcg gctgcttgca gggccggcac cgcccgcgcc
     7981 gcatccagcg cggcgacgag cttgtcgagg ctggcgacgg cgtcctgtgc cggcgtggcc
     8041 agcgtgtgca cgttgcagcc gtccggcacc agctcgccct gcttgccggg ataggcgaag
     8101 aacgagaccg gcgccttcgc gtccaccagc accaggtgct cgaagccggc cagctgcaac
     8161 gaggccagtt cggccaggta ggcgatgcgt tccaccggcg gcaacccggc gccgcgttcc
     8221 agccgggtcg ggaacacttc gcagaacagc ttggccccgt gctgcgcggc gatccgcgcc
     8281 gccgccagca ggcccggctc gcgcaaggcc tgcccgccaa gcaagagggc gcagcggccg
     8341 ccggcgcgga tggcatgggc gatcgcatcc acgcaggcat cgtcggcggg ttccggcgcg
     8401 ggggcgggcg gcggcggaca gggcacgccg ccttcgcccc agctgacgtc cgcgggcagg
     8461 accagggtcg cgacctggcc gggcaggccg cgcgcggcct tgatcgcatc gaccgcgtcg
     8521 cggcagagat cggcggtgct gcgcgaggtg cggacgaagc cgggcgacac gttgcgcgcc
     8581 acggtttcga tgtcggactg cagttgcgcg tccagcggca cgtgatgggt ggcgtggtcg
     8641 ccgacgatgt tcaccaccgg caccttgccc ttgcgcgcgt tgtgcaggtt ggcaagccca
     8701 ttgccgaggc cgcaacccag gtgcagcagg gtggcggcgg gcttgccggc catccgcgcg
     8761 tagccgtcgg cggcgccggt ggccacccct tcgaacagcg ccagcaccgc gcgcatgcgc
     8821 ggttccgaat cgagcgcggc gacgaagtgc atctcgctgg tgccggggtt gctgaagcag
     8881 acctcgatgc cggcatcggc cagggtctgc agcagggctt gggcgccgtt gggcatgggg
     8941 ggctccgggg gatgcgtggt caggcgcggt cgaggtgctg cgggccgatc tcggcgatgc
     9001 gggtgacgcc ggccagggcc atggtggtgg ccagttcatt ctgccaagtg gccagcagtc
     9061 cgcgcaggcc ggcttcgccg ccgccggcca gcgcccacgc ccacgggcgg ccgatcagcg
     9121 cgccgcgtgc gccgagggcg cacgcgcgga acaggtcgac cccggaacgc aggccgccat
     9181 cgaccaggac ttcggtgtcg gcgccgaccg cagcggcgat cgccggcagc cgcagcgcgg
     9241 tggcggcggc gccgtcgagc tggcgcccac cgtggttcga caccaccagg ccgtcggcac
     9301 cgacggccac tgccgcgcgc gcatcgtcga catcgaggat gcccttcaac agcagccgac
     9361 ccggccagcg cgcacgcagc cagtcgagtt ccgcccaggt gacgcccgga tcgaactggg
     9421 catcgatgaa ggcgcggaag gcatcgaggt cgcgcgcgtt cggcaattgc cgctgcaggt
     9481 tgccgaacag cagcggcttg ccgcgcagcg cgacatccca cagccagccg ggccgggcca
     9541 gcacctgggt gagccgcagc agcttcggcc gcaccccggg ctgcgccatg ccgttgcggg
     9601 tatcgcgatg gcgcatgccg ggcaatggca ggtcgacggt gaacaccagc gtgcggcacc
     9661 cggcggccca cgcgcgctgc agcaggtcgg cgaccagtcc gcggtcgcgc agccagtaca
     9721 gctggaacca gaacggtgcg ccggtgcctg ccgcgagttc ctcgatgctg cagatgccga
     9781 ccgtggacag cgtgaacggc acgcccgcgg cggtggccgc gcgcaacgcc tgcagctcgc
     9841 cacggcgggc cagcaggcca cccagcccca gcggtgccag cgccagcggc atccggcagg
     9901 catcgccggc cagggtgctg gcggtgtcga ctccggccac gtcgaccagc acccgctggc
     9961 ggatcccgat ccccggccac gcggcgacat tggcggccag ggtctgctcg gcgccggcgc
    10021 caccatcgac gtagtcggcg aggaagcgcg gcagccggcc acccgcgcgt gcgcgatggt
    10081 catcgctggt ggccggcatc gtgcggatca ccgataccgc ccttgcgtcc ggccagcagc
    10141 gcaggccgct gcctgcattt ggtgatcgct tccgcgtggg cctgcgcgcc gcgtgcgaaa
    10201 cgggcacgtc caccacccgc gcgcgttcgt cgctgcggtg cctgcgggcc tgcggtcgcg
    10261 gtggaagtcg atcatgctgg cctggtcgtg cgtcgtggcc aaggatacgc cggcccggtc
    10321 cgcgcatgcg tcgccggagc gggatgggcg cggtcctttc cggcgcggga tcccggcggc
    10381 gtcggcgcgc gcgcgcgcgt gccacccccc gatcgcggga tggtgccgcc cgcacccggc
    10441 cgcgatcatt gctgcctcag accaaggggg ttccatgttc cgtcatgcca tcgccggcct
    10501 cgtgctggcc agctccgcac tcgtttcgct ggcggtcccg gcggtcgccg atgccgggga
    10561 gccggtggtc gccgtccacg acatcgggaa cctcaagggc gtcaagcggg tcgcggtcac
    10621 gtccttcgtg gtgcagtacg tggtggcgca acagaccacc tcgcggggcg gcgccagctc
    10681 cggcatcgac ctgggcaagg acatcgatcc ggcgcgcctg cagcaggcga ccgaacagat
    10741 ctacgcgcag ttccttgccg acctcggcgc gtccgggatc gaagtggtgg cgccggaggc
    10801 cctcgcggcc tcgaaggggt tccgggagat gctgggcaag tcgccggcca cgccgttggc
    10861 gatgtcgacc tggagccgcg gcaaggatgg cggctacagc agcgtcttcc atgtgcccaa
    10921 gggcttgccg atggtcgttc gcgacgacta cgaatacctc aagggccgcg gcctcggcca
    10981 ggtcacggat ccgagcctga gcatcggcgg cggcatcaag ctcgccagca ccaactggcg
    11041 ctactacgac aaggcggtgc aggacgagct gcaggccgcg accctgctgg ttcgggtgtt
    11101 cgtgccgctg gcttacaacg agagcagtac ctcgatcgcc ggtccgtggc agaaggacag
    11161 cgcgaagacc accgccggcc tgcgcatcgg cgagcgcttc acgcgcatgg cggtggtgca
    11221 ggacgacgaa atcgccaaga tctacctcgg cgagccgttg ctggcgggcg attcgatcat
    11281 cgccaaccag ggcgcgaagt ccggcttcac cgccagcaag ctgctgttcg gcagcaagaa
    11341 caacgagagc ttcgcgctgg atgccgacca gtacttccag gacgtgccgg ccgcgtccgc
    11401 gaaggtcctg aatgccttca tcaaggcgat gaacgacaac cgctgaagca cggcgaggcc
    11461 ggcccctcgc cgttgcgggc gtcgggccgg cggtggctca ggcgccggtc gcgatggaac
    11521 gcgcggcggc gcgcgcggtg aggatgcagc cgggcaggaa ggtgccttcc agcgagcgtt
    11581 tgccgcaggc gccgccgccg ccgaagccgg cggcttcgcc cacgcagtac aggccgtcga
    11641 tcggctgctc gtcggcgtcc agcacccggc tgtgcaggtc ggtgcgcagc ccgcccaggc
    11701 tcttgcgggt gatcagctgc atgtggatgg cgatgtacgg tcccgcgccg ggctgttgca
    11761 gcggtgccgg cttgcaggtg cgcagggcgt cggggcccca ctggcgtgca tgcaggatgc
    11821 ggcgcacctg cgcgtcgtcc tgcgggttcg cgcctgcggc gaagtgcgca tcgaaggcat
    11881 cggcggtggc ctgcagggtg gccggatcga tggcgtgcga gcaggtcagg gcgttcatct
    11941 tcgcggccag ttccgccagg gtgtcggcga ccaggaagtg cgcgctctcg cgctgcatct
    12001 gccgcaccag ccggtggttg cccagcaggg tttccttgag gaagcgcagg aactgcttgt
    12061 cgcggatgcg ggggttgtgt tccgccccgg agatcgcgaa ctccttcgcg gcgatgcgcc
    12121 agttgagcag gtgccaggtc cacggctttt cctgcgccgc cacccgctgg cacagccaat
    12181 gggtgtcgaa gccggtgacc agcggctccg ggccgatgcg ccggcccgtg tggtccagcc
    12241 acagcgcgga cttgcacggg atcgccgaca gtccgtggcc gtcgaaatgc gggaacggat
    12301 gcggaaagcc ggcggcgtag ttccacatct cgccggcgtg ggtgacctgc gcaccaagcc
    12361 cggaggcgat ctggtgcagg cggccatcgg cgaacggatg cgcgccgttg agcatgcttg
    12421 cgggacacgg gcggtcctgc ggccagttgc cccggacctg cgcatggctg ccgttgatgc
    12481 cgcccattgc cagcaccacc accggcgccg ccagccgcac ttcgccgccg gtggcttccc
    12541 cgatcgccag cgcaccggcg atcctgccgc cggcatgctc gagcgcggcg acgcgatggc
    12601 catgcagcag gcgcaacctg ccaccggcgc cggcctcgcg cagggcggcg atcatccgcc
    12661 gggtcagctc gcgcgaggtg ccccagacga cgtggtagcg gggcacgctg ttgccttcgc
    12721 cgtcgcgccc gcgctcgacc cagttcaccg ccggcatgaa gcggatgccg tgcgcctgca
    12781 gccagtcgtg cacctgcggc cgcgactgct cgacgtagtg gcgcgcccat tgcatcggga
    12841 agcggtcgtc gggatccatt tcgccgaacc gcatccagtc gcgcagggcg atctcggggc
    12901 tgtcgatgat cttcttgctt gcctgcagtg gcgtccccac cagcgccatg ccgccgaaag
    12961 cccacagcgc cagcccgccg aagcgttcgg gagtgtcgcg gtccaccagg gtcacccgct
    13021 ggccggcgcg cagcgcctcc agcgcggtga cgatgcccgc cagcccgccg ccgatgacca
    13081 ggacatccga ttgcagtgcc gtcatgccaa ccccctgggc gcatcatcac ccgcgccgga
    13141 gatcgaaaga tagccgcttg cggcagccac cgatggcgcc gtccgcggcg cccgcgtcag
    13201 cgcgcgagac cgcccagcag cggtgccggc agcaggtcga tgccggccgc gaccaggccc
    13261 gcggcggggg cgtccagcgg catcacggcc aacgccaggt cttcctcgac gcaggccacg
    13321 ctgccgatgt cgcgcccgtc cgcgctgatc gcgctgcctt cgggcagcgg cgcgccggcg
    13381 tggaacagcg ccagcccgcg tttcacctgg ccgaggaaat gggtgcgcgc cacgatttcc
    13441 tggcccgggt agcagccctt cttcacgctg taggcgcgca ggcgatccag cgacaggtgc
    13501 tgcggcgtcc atgcctcgag ctgcgcatcg cgcaggcgcg gcagcccgtg ctggaggtcg
    13561 aagccacgcc agcggctggc gagcgcggca tcggcttcgg ccgcggcgtt ggcgatgcgc
    13621 aggcagcgcg ggccgccgtc ggcgccgaga tccagttcga atgcgccatc ggcctgcgcg
    13681 atcgtcgagc ccgatgccag tgccggtgcc ccgaagcggc cattcgcgtg caggtcgtcg
    13741 cgcggctgca gccgcacctt gctgcggaaa acgaaccgtt gcaattgcgt cgccaattcg
    13801 cccgcaccgg catccggcaa gaccagccac agggtttcgg cgtcttgccg gagcagggcg
    13861 aacagcgcga tcacgcggcc tttcggcgtc agccagccgt tccagtgcca tccgccatcg
    13921 accagcgcgg cgacgtcgtt catgaactgc gcctgcgcga acgcgactgc atcgcggccg
    13981 ccgatggcca gcacatcatg gccgggtagc cggaattcct gcgccggtgc ggttcgtggc
    14041 ttgtgggaca tggggtcggg acttaaactt gcggggttgt gaatgcgtcg atcataggcc
    14101 aaacccctcc cagccccgag tccgtcgagg ccgcggttcc gcacctggaa cccgtgccgc
    14161 gtgccgcttc ggagcccgcc accgccatcg accccgcggt cgagatcggc gggcgcgacg
    14221 gccccgagcc gacccgctac ggcgactggg aaaagaatgg ccgctgcatc gacttctgag
    14281 ccatcgccac gcggcgcaag ccgccccgcc ggagagcgca gcgcccatgg ccgctcctga
    14341 tacgcacgtc cgccaacgcc cgttgtcacc gcacctgcag gtctacaggt ggcaggtgca
    14401 gatggcctcc tcgatcctgc atcgcgccac cggcgtgatc ctctcggttg gcgcgctggc
    14461 gctggtctgg ggcctgctgg cgctggccgg cggcccggaa ccgtggcagg cgttcatcgc
    14521 ttgcgtcggc tcgccgcttg gcaccgtcgc cctgttcggc ttcagctggg cactggccta
    14581 ccacctgctc aatggcatcc gccacctggt gcaggacgcc ggccacggat tcgcgatcgc
    14641 ggatttcgtg cgcagcagct gggtctcgat catcggcagc gtgctgctgg tggcggcggt
    14701 ttgggccatc gtgctggtgc gctgggggca ggcatgaaca acgcgaacga tctgcgcacc
    14761 ccgctcaagc gtgcgcgcgg gctgggctcg gcgaaggatg gcaccggcca tttcacctgg
    14821 cagcggatca ccgcgatcgc ggtggcgttg ctgggcatct acctgctggg cttgctgatc
    14881 ggcatcggcg gcgcggacta cgaccgcgcc cgcatgatcg tcgccgaccc gttgaacgcg
    14941 accctgctgg tcgcgttcgt gatcgccgcc ttctggcacg ccaggcttgg cctgcaggtg
    15001 gtcatcgagg actacgtcca taccccgctg ctggccggtg ccgcgcacct cgccagcctc
    15061 ttcgtctgtg cactcgccgc catcggcggc gtgctcgcga tcgtccgcat cgcgctggga
    15121 gcctgattcc gtgtccgcct acagcaagcc tgcctacagc atccaggaac acaagttcga
    15181 catgatcgtg gtcggcgccg gcggtgccgg gctgcgcgcc accttcggcc tcgccgagaa
    15241 gggcctgcag accgcctgca tcagcaaggt cttcccgacc cgttcgcaca cggtggcggc
    15301 gcagggcggc atctccgc
//
LOCUS       DAORUK010000040        15276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_3661_length_15276_cov_18.358781, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000040 DAORUK010000000
VERSION     DAORUK010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15276
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3661_length_15276_cov_18.358781"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /gene="pilM"
                     /locus_tag="PK818_04255"
     CDS             complement(<1..679)
                     /gene="pilM"
                     /locus_tag="PK818_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HQY81981.1"
                     /translation="MGLVTKTQSPLIGVDISSTAVKLLQLARTGDRYRVEHYAVEPLP
                     PNAVVEKNIVEVEAVGEAIRRAVSRSGSRTKFAAAAVSGSSVITKSIPMPSDLDEDEM
                     ESQIELEAVNYIPYPIEEVNLDFEVIGPMPGNAEMVQVLLAASRSENVEARASALEVG
                     GLVAKVIDVEAFAIENAYALMAGSINVPNNGLVALVDVGATMTTLNVLRGGRSIYTRE
                     NVFGGKQL"
     gene            932..3400
                     /locus_tag="PK818_04260"
     CDS             932..3400
                     /locus_tag="PK818_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014504440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HQY81982.1"
                     /translation="MPRLPRTLRLVLLGGVTLAVIGAIAIALLVASLSAKLPDVQALR
                     TTELQEPMYVYASDGRLMGLFGEMRRYPVDIKDVPERVQRAFLAAEDARFYQHHGVDY
                     KGVARAVWLLATTSDKRVPGGSTITQQVARQYFLDSEYSYARKLREMLLAMKMERELG
                     KDEIFGLYLNKSFFGNRAYGVAAAAEFYYGKTLAELTLDEAATLAGTPKFPSSGNPLD
                     NPTRNRERRDHYILPRMAALGYISAAEAEAAKAVPMHASPHERPIEVHAPYVAEMVRR
                     EMIARFGEDVVSKGYHVTTTIDPVLQAAADKAVADGMRVYDHRHGWRKPDRQVEIPAG
                     ADPMAIARLLRNTPAQGGLLPAVVVGSGSDGVRVVLADGSEVTLGSSATGWTGKSASA
                     LAQRGDVVRVRRGNADKPGAAPEWVIDQIPRAQTALVSLDADTGALRALVGGFSYAGN
                     KFNRATQARRQPGSSFKPFLYAAAFERGFNPASIVLDAPVVFKDRAGHTWRPQNDSGN
                     FAGPMRVREALVQSRNLVSVRLLDAIGVEYARRYISHFGFEEQRLPPNLSMSLGTASL
                     TPLSVARGYAAFANGGFLVTPWFIDSVRDRSGKDVFREKPATACPSCALVQAPGQPVA
                     TVVQSTTVDGFDFGPAGGTPGAAAPPAQAATVSKPRPARPVDPAMVVAPRAIDPRVAW
                     QLQSMMRDVVKRGTATDARVLGREDVGGKTGSTNDHRDAWFSGFGGPYVTTVWVGRDD
                     FKSLGYREYGGKAALPIWISYMKTALKEEPPRDLEPPAGMVEVKANGVVEWLKVEDQE
                     RIQDELDAMPDQDAQPDEEAFDIF"
     gene            3499..4170
                     /locus_tag="PK818_04265"
     CDS             3499..4170
                     /locus_tag="PK818_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY81983.1"
                     /translation="MHNTRPHARVHAHTHAQTRTRERRQHLAFEAARLMAEGGIRDYH
                     QAKLKAAQRLGIHDDASLPRNCEIDEALREYQRLFLGSGQARELRRRREAALRALEFF
                     HAFEPRLVGPVLDGTADANAAVALHLHGDDADAVPRWLDQHGVPAQARSRHLRLDRER
                     AADFPVWLFSAEDLSFDATVLPADVLRQAPLSGVDGRPMRRASAAQLRMLLAEDEIAG
                     YEALP"
     gene            complement(4247..5551)
                     /locus_tag="PK818_04270"
     CDS             complement(4247..5551)
                     /locus_tag="PK818_04270"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HQY81984.1"
                     /translation="MTQSGNSQPDQITLNAGDKSVAMPLLHPTLGQPCIDIARLPRET
                     GCFTYDPGFGATASCKSAITYIDGDAGVLLYRGYPIEQLAEKSSFLEVAYLLMHGELP
                     NAGAFSKFEHEVTHHSMMHEAFRTFLYGFRHDAHPMAMLTGMLGSLASFYHNDLDLED
                     PEQRRLAAVRLIAKVPTIAAACYRYSIGWPIRYPRNNLEYTTRFLHMMKEVPSEPLEL
                     NPVAAKAMDLLFILHADHEQNASTSTVRLVGSTGANPYVSVAAGVAALWGPAHGGANE
                     AVLKMLAEIGRPEDVRSAIDKAKDKESGFRLMGFGHRVYKNFDPRAKIIREMCHKVLA
                     DLGVNDPLLEVAMRLEEAALKDDYFVQRKLYPNVDFYSGIIYKALGIPVEMFTVMFAI
                     ARTAGWVSHWLEQQEDPENRIGRPRQIYTGAANRDFVDIGAR"
     gene            complement(5758..6000)
                     /locus_tag="PK818_04275"
     CDS             complement(5758..6000)
                     /locus_tag="PK818_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HQY81985.1"
                     /translation="MKAGIHPEYREVVFQDVTSDFKILTRSTLASKETVKWEDGNEYP
                     LIKVEVSSSSHPFYTGQNKVIDTSGRVDKFRKRYQK"
     gene            complement(6068..7009)
                     /locus_tag="PK818_04280"
     CDS             complement(6068..7009)
                     /locus_tag="PK818_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HQY81986.1"
                     /translation="MTNPRIPLLIDTDPGVDDALALLMAFNDRRHQVVGLTIAAGNVG
                     LAHTVANALKLCEVCGVDVPVFAGAADPLLHPAEDAAFVHGRDGFGDTGHAPAARAAE
                     SEHAALAILRLSHAHAGKLLLVALGPLTNVALALKLDPTLPDRVARCVVMGGAVSAHG
                     NISAAAEFNIAFDPEAAHIVFTAFPRIDVADWEAVIAHGFLHADAERWLAADSPRAAF
                     YASISAHNRAWSRAGRGERWHAADALAMAHALEPEGALEVLERPLAVELEGRHSRGAT
                     IVDWNRRHGRPDNAAILMRYDQARFEGLIERALAATG"
     gene            complement(7027..9132)
                     /gene="recG"
                     /locus_tag="PK818_04285"
     CDS             complement(7027..9132)
                     /gene="recG"
                     /locus_tag="PK818_04285"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HQY81987.1"
                     /translation="MVRSARAGPTLAASGAAPLASLPGVGPALAEKLAARGLLTLQDL
                     WLHLPRQYEDRTSLAPIRQLQAGVAVQVEGRVEAVERGFRYRPMLRVALSDESRATLV
                     LRFFHFRAQQAAQFAPGTRVRAYGTPRPGQHGLEMVHPSYRVLGEGEHGLGEALDPVY
                     PEVEGIGPANLRKLVGLALERLPDADTLELLPLEVRDALQLPGLRDALVTLHRPPRDA
                     DVAALQAGLHPAQRRLALEELLAHQLSLRRQRIALQQLGARPLRGKGALVERLRASLP
                     FALTGAQQRVYAQVRDDLAQPRPMLRLVQGDVGSGKTVVAAMAALLAVEAGRQAALMA
                     PTELLAEQHLANLRGWLEPLGVRVCWLAGKVTGKARAQALAQVASGAAQVVVGTHALM
                     QEGVAFHDLALAIVDEQHRFGVHQRLALRDKGQRDAVPHQLVMTATPIPRTLAMSAYA
                     DLDVSAIDELPPGRTPVTTIALAAERRPELIERIRAACAEGRQAYWVCTLIDDSDEVV
                     AQAAQSTFEALTLALPELRIGLVHGRQKAADKQATMRAFKQGELGLLVATTVIEVGVD
                     VPNASLMVIENAERLGLAQLHQLRGRVGRGSAASSCVLLYQSPLSAMARERLDTLRRS
                     NDGFEIAEKDLQLRGPGELLGTRQTGLAAFRIADLARDADLLPRVHALADQLLQAAPA
                     TAAAVVQRWVGGAARYASA"
     gene            complement(9136..9519)
                     /locus_tag="PK818_04290"
     CDS             complement(9136..9519)
                     /locus_tag="PK818_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQY81988.1"
                     /translation="MPRTPIHSDHAPAAIGPYSQATRAGNLVFLSGQIPLDPATGNLV
                     EGDITVQARRAFDNLEAVCAAAGGSMDDIARVGLYLTDLSQFGAVNAVMAEYFAAPYP
                     ARSTIEVPALPKGAVFEVDAMMVLP"
     gene            complement(9614..11791)
                     /locus_tag="PK818_04295"
     CDS             complement(9614..11791)
                     /locus_tag="PK818_04295"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HQY81989.1"
                     /translation="MSPGDPALALPTPPAPEDAAVPDYVQALERAADYLPEAQRALLR
                     RAWAVGASAHVGQLRKSGEPYITHPVAVAQVLAEQGLDVETLVAAILHDTIEDTPLSR
                     ADIATQFGETVAGLVDGVTKLDKLHFADRQEAAAESFRKMLLAMSRDLRVILIKLADR
                     LHNMRTLGAQSVDSRRRIASETLDIYAPIAQRLGMNLIKSELQDLGFRALHPWRHAVI
                     EKRIRSQPLVRREALAKIEAQLAQKLAMEKVEHRLVGRIKTPWSIYTKMRAEHRSFAQ
                     VMDVFGFRVVANSVAECYHALGVVHSVYKPLDGRFRDFIAIPKANGYQSLHTVLFGPY
                     GSPIEVQIRTREMDLVAERGVAAHWAYKHGEDPGNSAQGRASAWIANLVESQRGTGSS
                     LEFLENVKVDLFPDEVYLFTPKGDILALPRNATALDFAYAVHTDVGNHAVAARVDRKL
                     APLRAKLASGQSVEIITAKSAAPAPQWLEFVVSGKARTAIRHQLKHLEHEDAVQLGHR
                     MLDRALGTLGTSLDRLPPILLDAYLAESRFPRLEALLADIALGNRMPAQVAQALATPE
                     QEPDLLRSAQAVPQERILITGAERGVVSFAQCCMPIPGDEIMGYHTAGKGIVVHRLEC
                     PNVGEYRKSPERWVQIGWDRQVSGDFDAALRIEVENRPGVLAQVAAAIAHAESNIDRV
                     EYLERDTRVAVIRFAIEVADRKHLADVIRRIRRLNVVHGVQRL"
     gene            complement(11898..12197)
                     /gene="rpoZ"
                     /locus_tag="PK818_04300"
     CDS             complement(11898..12197)
                     /gene="rpoZ"
                     /locus_tag="PK818_04300"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HQY81990.1"
                     /translation="MARITVEDCLEVVDNRFELVMMAAKRARQLAGGVEPKLDNSEAN
                     DKPTVLALREIAAREIDLGYIDAVEKSERERKEREALEWAAAEVVAGDDDMKGDD"
     gene            complement(12307..12939)
                     /gene="gmk"
                     /locus_tag="PK818_04305"
     CDS             complement(12307..12939)
                     /gene="gmk"
                     /locus_tag="PK818_04305"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HQY81991.1"
                     /translation="MRGTLYIVAAPSGAGKSSIVNAVLARDHNISLSISFTSRQPRPG
                     ERHAEHYHFISADEFEAMVAAGDFFEHARVHGDWKGTARQSVEPQLMAGRDVLLEIDW
                     QGARQVRSRIPDAVSVFILPPSRAALETRMRKRGQDSEEVIAQRLAAARDEMSHYGEF
                     DYVIVNEDFETAVGEMCSIFTASRLRKDAQVARHARLITSLLADDPGQPG"
     gene            complement(12943..13806)
                     /locus_tag="PK818_04310"
     CDS             complement(12943..13806)
                     /locus_tag="PK818_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005996763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HQY81992.1"
                     /translation="MIRSMTAFASGERSTEWGVLGCELRAVNHRFLELGLRLPEELRA
                     FEPILRERIGARISRGKLDLAMRLRAPESDAGLHLNEALVGELSRLNLDLAQRFPGLR
                     TSLTELLQYPGVLQGRGLDQEALQREVLALLDVVLADFVATREREGGKLAAVIGERAA
                     AIAGIAADVRTLVPLIRAGQRHKLEARLADLSQPADPGRIEQELVLWLQKLDVDEELD
                     RLDSHLAELRRILKGGKEPVGRRLDFLLQEFNREANTLGSKSVDARTSNAAVELKVLI
                     DQVREQIQNIE"
     gene            13886..14614
                     /gene="rph"
                     /locus_tag="PK818_04315"
     CDS             13886..14614
                     /gene="rph"
                     /locus_tag="PK818_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HQY81993.1"
                     /translation="MSFSRPSGRAPEQMRAVSITRGYTRHAEGSVLVAFGDTRVLCTA
                     SVENRVPGFLRGKGEGWITAEYGMLPRATHTRGDREAARGKQGGRTLEIQRLIGRSLR
                     ACVDRAALGERTITLDCDVLQADGGTRTAAITGAYVALVDAVRWLRAKKEIARDPVIG
                     AVAAVSAGVYRGVPVLDLDYPEDSDCDTDMNVVMNDGGGFIELQGTAEGHAFRRDELD
                     ALLALAERGCGELFAAQRAALATP"
     gene            14611..15195
                     /gene="rdgB"
                     /locus_tag="PK818_04320"
     CDS             14611..15195
                     /gene="rdgB"
                     /locus_tag="PK818_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HQY81994.1"
                     /translation="MKLVLASSNAGKLAELRGLLGDGFELHVQSEFGVADAAETGLSF
                     VENAILKARHAARATGMPALGDDSGLCVDALHGAPGLYSARYAGAHGNDEANIDKLLH
                     ELDGVDDARRGARFVCVLALVRDAHDPQPLLAEGTWHGRILRERRGDNGFGYDPVFFS
                     PAHGSSAAELPATIKGRDSHRGLALAKLRALLLA"
BASE COUNT         2271 a         5381 c         5290 g         2334 t
ORIGIN      
        1 tgagctgctt gccgccgaac acgttctcgc gggtgtagat gctgcgaccg ccacgcagga
       61 cattgagggt ggtcatcgtc gcgccgacat cgaccagtgc caccagtccg ttgttcggca
      121 cgttgatgct gccggccatg agcgcgtagg cgttctcgat ggcgaacgcc tcgacatcga
      181 tcaccttcgc gaccagcccg ccgacttcca gcgcggaggc gcgcgcctcg acgttctccg
      241 agcgcgacgc ggccagcagc acctgcacca tttcggcgtt gccgggcatc gggccgatca
      301 cctcgaaatc gaggttcact tcctcgatcg ggtacgggat gtagttgacc gcctccagct
      361 cgatctgcga ctccatctcg tcttcgtcga gatcggaggg catcgggatg gatttggtga
      421 tcaccgagga gcctgaaaca gcggcggcgg cgaacttggt gcgggagccg gagcgcgaca
      481 cggcacggcg gatcgcctcg ccgaccgcct ccacctcgac gatgttcttc tccaccaccg
      541 cattcggcgg tagcggttcc acggcgtagt gctcgacccg gtaacggtcc ccggtgcgcg
      601 ccagctgcag gagcttgacc gcggtcgaac tgatgtcaac gccgatcagc ggcgattgcg
      661 ttttcgtcac gagacccacg gtatctcccc ttcccacggg cgcttacgcg cactaggtgc
      721 ttcagtacat gaataacgga cttttcacac aatggcaacc catgtcactt atcgaccctg
      781 cgcgccacgc ccgaaatccc gccttcggtt cgtacctgct ctccccctaa cgcagcccat
      841 tggtcaaacc ggccgccccc tgcatgccga tccccactga ggcctcctct atactccgcg
      901 accatcaaac ccgcaatcgg aacctgtgtc gatgccccgc ctcccccgca ccctgcgcct
      961 cgttttgctg ggcggcgtca cgcttgcggt gatcggcgcc atcgccatcg cgctcctggt
     1021 cgccagcctg tccgccaagc tgccggacgt gcaggccctg cgcaccaccg aattgcagga
     1081 gccgatgtac gtgtacgcca gcgacggtcg cctgatgggc ctgttcgggg aaatgcgccg
     1141 ctacccggtg gacatcaagg acgtgccgga acgtgtccag cgggcgttcc tggccgccga
     1201 ggacgcgcgc ttctaccagc accacggcgt cgactacaag ggcgtggcgc gggcagtctg
     1261 gctgctggcg accaccagcg acaaacgcgt gcccggcggc tccaccatca cccagcaggt
     1321 ggcgcgccag tacttcctgg actccgagta cagctacgcg cgcaagctgc gcgagatgct
     1381 gctggcgatg aagatggagc gcgaactcgg caaggacgag atcttcggcc tctacctcaa
     1441 caagagtttc ttcggcaacc gcgcctacgg ggtggcggcg gcggccgagt tctactacgg
     1501 caagaccctt gccgaactga ccctggacga ggccgcgacc ctcgccggca cgcccaagtt
     1561 cccgtccagc ggcaacccgc tggacaaccc cacccgcaac cgcgagcgcc gcgaccacta
     1621 catcctgccg cgcatggccg cgctgggtta catcagcgcc gccgaggcgg aggccgcgaa
     1681 ggcggttccg atgcacgcga gcccgcacga gcgcccgatc gaggtgcatg ccccctacgt
     1741 ggcggagatg gtgcgccggg agatgatcgc ccgcttcggc gaagacgtgg tcagcaaggg
     1801 ctatcacgtc accaccacca tcgacccggt gctgcaggcc gcggcggaca aggcggtggc
     1861 cgacgggatg cgcgtgtacg accatcgcca cggttggcgc aagcccgatc ggcaggtcga
     1921 gataccggcc ggtgccgacc ccatggcgat cgcccggctg ctgcgcaaca cgcccgcgca
     1981 aggcggcctg ctgccggcgg tggtggtcgg cagcggcagc gatggcgtgc gggtggtgct
     2041 cgccgatggc agcgaggtga ccctgggcag cagcgcgacc ggctggaccg gcaagagcgc
     2101 cagcgcgctg gcgcaacgcg gcgacgtggt gcgcgtgcgc cgcggcaatg ccgacaagcc
     2161 tggcgcagcg ccggaatggg tgatcgacca gattccgcgc gcgcagaccg cgctggtgtc
     2221 gctggatgcc gacacggggg ccttgcgcgc actggtcggc ggcttcagct atgccggcaa
     2281 caagttcaac cgcgcgaccc aggcgcgccg gcagccgggt tccagcttca agccgttcct
     2341 gtacgcggcc gcattcgaac gcggcttcaa cccggcctcg atcgtgctgg acgcaccggt
     2401 ggtgttcaag gatcgcgccg gccacacctg gcgtccgcag aacgacagcg gcaacttcgc
     2461 cggcccgatg cgcgtgcgcg aggcgctggt gcagtcgcgc aacctggtgt cggtgcgcct
     2521 gctggatgca atcggcgtcg aatacgcgcg ccgctacatc agccatttcg gcttcgagga
     2581 acagcggctg ccgccgaacc tgtcgatgtc gctggggacc gcgtcgctga ccccgctgtc
     2641 ggtcgcgcgc ggctatgcgg cctttgccaa tggcggcttc ctggtcacgc catggttcat
     2701 cgacagcgtc cgcgaccgca gcggcaagga tgtcttcagg gagaagccgg cgaccgcctg
     2761 cccgtcctgc gcgctggtgc aggcgcccgg ccagccggtg gccacggtgg tccagtccac
     2821 cacggtcgac ggcttcgact tcggacccgc cggtggcaca ccgggcgctg ccgcgccgcc
     2881 ggcgcaggcc gccacggtct cgaagccgag gccggcccgg ccggtggatc cggcgatggt
     2941 ggtggcgccg cgcgcgatcg acccgcgggt tgcgtggcag ctgcagtcga tgatgcgcga
     3001 cgtggtcaag cgcggcaccg ccaccgatgc ccgcgtgctc ggccgcgagg acgtcggcgg
     3061 caagaccggt tccaccaacg accaccgcga cgcctggttc tccgggtttg gcggccccta
     3121 cgtcaccacc gtctgggtcg ggcgcgacga cttcaagtcg ctgggctacc gcgaatacgg
     3181 gggcaaggcg gcattgccga tctggatcag ctacatgaag acggcgctga aggaggagcc
     3241 accgcgcgac ctcgagccgc cggcggggat ggtcgaggtc aaggcaaatg gcgtggtcga
     3301 gtggctgaag gtggaagacc aggagcgcat ccaggacgaa ctggatgcga tgcccgacca
     3361 ggacgcgcag ccggacgagg aagcgttcga catcttctga tcgatgtccg ccgccagcgc
     3421 cacggcggcg cgcagcccgc tggtgcgctg ccggccttgc tcgggtacag tcggggcagg
     3481 ccgcaccggg gacgcgcgat gcacaacacc cgcccgcatg ccagggtcca cgcgcacacc
     3541 catgcgcaga cccggacccg cgaacgccgc cagcacctgg cgttcgaggc ggcgaggctg
     3601 atggccgaag gcggcatccg cgactaccac caggccaagc tcaaggccgc gcagcggctt
     3661 ggcatccatg acgatgcctc gctgccgcgc aactgcgaga tcgacgaggc gctgcgcgag
     3721 taccagcgcc tgttcctcgg cagcggccag gcccgcgagc tgcgccgccg ccgtgaagcg
     3781 gccttgcgcg cgctggagtt cttccacgca ttcgagccgc ggctggtcgg tccggtgctg
     3841 gacggcactg ccgacgccaa tgccgcggtc gccctgcacc tgcacgggga cgatgccgac
     3901 gcggtgccgc gctggctgga ccagcatgga gtgccggcgc aagcgcgcag ccgccacctg
     3961 cggctggacc gcgagcgcgc cgccgacttc ccggtctggc tgttttccgc ggaagacctc
     4021 agcttcgacg ccaccgtgct gccggccgac gtgctgcggc aggcgccgct gtcgggggtc
     4081 gacggcaggc cgatgcgccg cgcatccgcc gcgcagctgc ggatgctgct ggccgaggac
     4141 gagatcgcgg gctacgaagc cctgccctga acccgtcttg ccacgcccgc aacgcaaggc
     4201 gccccgcgat gcggggcgcc cgttcgttgc gcgatgcccg ctgcggtcag cgcgcgccga
     4261 tgtccacgaa gtcgcggttg gcggcaccgg tgtagatctg gcgcgggcgg ccgatgcggt
     4321 tttccgggtc ctcctgctgc tccagccagt ggctgaccca gccggcggtg cgcgcgatcg
     4381 cgaacatcac cgtgaacatc tccaccggga tgccgagcgc cttgtagatg atgccgctgt
     4441 agaaatcgac gttcgggtag agcttgcgct gcacgaagta gtcgtccttc agcgccgcct
     4501 cttccagccg catcgcgacc tccagcagcg ggtcgttgac gccgaggtcg gccagcacct
     4561 tgtggcacat ctcgcggatg atcttcgcgc gcggatcgaa gttcttgtac acgcgatggc
     4621 cgaagcccat caggcggaag ccggactcct tgtccttggc cttgtcgatc gcggagcgga
     4681 cgtcctccgg gcggccgatc tcggccagca tcttcagcac cgcctcgttg gcgccgccat
     4741 gcgccgggcc ccacagcgcg gccacgccgg cggcgacgct cacatacgga ttggcgccgg
     4801 tcgagccgac caggcgcacg gtcgaggtgg atgcgttctg ctcgtggtcc gcgtgcagga
     4861 tgaacagcag gtccatcgcc ttggccgcga ccggattcag ctccagcggc tcgctcggca
     4921 cttccttcat catgtgcagg aagcgggtgg tgtattcgag gttgttgcgc gggtagcgga
     4981 tcggccagcc gatcgagtag cggtagcagg cggcggcgat ggtcggcacc ttggcgatca
     5041 ggcgaaccgc ggcaaggcgg cgctgctcgg gatcctcgag gtcgaggtcg ttgtggtaga
     5101 agctggccag cgaacccagc atgccggtca gcatcgccat cggatgggcg tcgtggcgga
     5161 agccgtagag gaaggtgcgg aacgcctcgt gcatcatcga gtgatgcgtc acctcgtgct
     5221 cgaacttcga gaacgcgccg gcgttcggca gctcgccatg catcagcagg taggcgacct
     5281 cgaggaaact ggacttctcg gccagttgct cgatcgggta accgcggtac agcagcaccc
     5341 cggcatcgcc gtcgatgtag gtgatcgcgg acttgcagct ggcggtggcg ccgaacccgg
     5401 ggtcgtaggt gaagcagccg gtttcccttg gcaggcgggc gatgtcgatg cagggctggc
     5461 ccagggtcgg gtgcagcagc ggcatggcca cggacttgtc gccggcgttg agggtgatct
     5521 ggtcgggttg gctgttgcct gattgagtca cggattcgct cctgtggggt ccgcacggcg
     5581 tcgccggccc gcaggccatg cgccgccgca tgggaagccg aaattatcgc acaggcgaaa
     5641 ccgagcccgg actcggacga aagtcgaatg cagggcccgc cgggatccga cccggcacgg
     5701 ccggaaacaa aacggccacc gcaagggtgg ccgttcggga actgcgctgg caggcgctta
     5761 cttctggtag cgcttgcgga acttgtcgac gcggccgctg gtgtcgatca ccttgttctg
     5821 gccggtgtag aacgggtgcg aggacgacga cacttccacc ttgatcagcg gatattcgtt
     5881 gccgtcctcc cacttcacgg tttccttgga ggcaagcgtg gagcgggtca ggatcttgaa
     5941 gtccgaggtc acgtcctgga agacgacttc gcggtattcg ggatggatgc cggctttcat
     6001 ggcgtaaacc tgagttggag cgggacagag ccgcgcatta taggggcatg cgtggcaccc
     6061 gcgcaagtca tcctgtcgcc gccagcgcgc gctcgatcag gccctcgaac cgcgcctggt
     6121 cgtagcgcat caggatcgcg gcgttgtcgg ggcgaccgtg gcgccggttc cagtcgacga
     6181 tggtggcacc gcggctgtgg cggccctcaa gctccaccgc cagcggccgc tccagcacct
     6241 ccagcgcgcc ttcgggctcc agcgcgtggg ccatcgccag cgcatcggcg gcgtgccagc
     6301 gctcgccgcg gccggcccgc gaccatgccc ggttgtgcgc cgagatgctg gcgtagaacg
     6361 ccgcccgcgg cgagtccgcc gccagccagc gttcggcgtc cgcatgcagg aagccgtgcg
     6421 cgatcaccgc ttcccagtcc gcgacgtcga tccggggaaa cgcggtgaac acgatgtgcg
     6481 ccgcttccgg gtcgaacgcg atgttgaatt ccgccgcggc gctgatgttg ccgtgcgcgc
     6541 tgaccgcccc gcccatgacc acgcaacggg ccacgcggtc cggcagggtc gggtccagct
     6601 tcagcgccag cgcgacattg gtcagcgggc ccagcgccac cagcagcagc ttgccggcat
     6661 gcgcatgcga tagccgcagg atcgccagcg cggcatgctc ggactcggcg gcgcgcgccg
     6721 cgggcgcatg gccggtgtcg ccgaagccat cgcggccgtg cacgaacgcc gcgtcctcgg
     6781 ccgggtgcag caacgggtcg gcggcgccgg cgaacaccgg cacgtccacc ccgcagacct
     6841 cgcacagctt cagcgcgttg gccacggtgt gggcgaggcc gacgttgccg gcggcgatgg
     6901 tcaggccgac cacctggtgg cggcggtcgt tgaacgccat cagcagggcc agcgcgtcgt
     6961 ccacgccggg gtcggtatcg atcagcaacg gaatgcgcgg gttcgtcatc ggccgattat
     7021 ggcgcctcac gccgacgcgt agcgtgcggc gccgccgacc cagcgctgga cgaccgccgc
     7081 ggcggtcgcc ggcgctgcct gcagcagctg gtcggccagt gcatgcacgc gcggcagcag
     7141 gtcggcatcg cgggcgaggt cggcgatccg gaatgccgca agtccggtct ggcgggtgcc
     7201 cagcagttcg cccgggccgc gcaactgcag gtccttctcg gcgatctcga agccgtcgtt
     7261 gctgcgccgc agggtgtcca ggcgctcgcg cgccatcgcc gacagcggcg actggtacag
     7321 cagcacgcag ctcgaggccg cgctgccgcg cccgacccgt ccgcgcagct ggtgcaactg
     7381 cgcaaggccc agccgctccg cgttctcgat caccatcaag gacgcattcg gcacgtccac
     7441 cccgacctcg atcaccgtgg tcgccaccag caggcccagc tcgccctgct tgaaggcgcg
     7501 catcgtcgcc tgcttgtccg cggccttctg gcgcccatgc accagcccga tgcgcagttc
     7561 cggcagcgcc agcgtcagcg cctcgaaggt ggattgcgcg gcctgcgcga cgacttcgtc
     7621 ggagtcgtcg atcagggtgc atacccagta ggcctggcga ccctccgcgc aggcagcgcg
     7681 gatgcgctcg atcagctccg gccggcgttc ggcggcgagg gcgatggtgg tgacgggggt
     7741 gcgcccgggc gggagttcgt cgatcgcgga gacgtcgagg tcggcgtatg ccgacatcgc
     7801 cagcgtgcgc gggatcgggg tggcagtcat caccagctgg tgcggcaccg cgtcgcgctg
     7861 gcccttgtcg cgcagggcca accgctggtg cacgccgaag cggtgctgtt cgtcgacgat
     7921 cgccaacgcc aggtcgtgga aggccacgcc ctcctgcatc agcgcatggg tgccgaccac
     7981 cacctgggcg gcgccggatg ccacctgcgc aagcgcctgc gcacgggcct tgccggtgac
     8041 cttgccggcc agccagcaca cgcgcacgcc aagcggctcg agccagccgc gcaggttggc
     8101 caggtgctgc tcggccagca gttcggtcgg cgccatcagc gcggcctggc ggcccgcctc
     8161 caccgccagc agcgccgcca tcgccgcgac cacggtcttg ccgctgccga cgtcgccctg
     8221 caccagccgc agcatcggcc gtggctgcgc caggtcgtcg cgcacctgcg catagacgcg
     8281 ctgctgcgcg ccggtcagcg cgaacggcag cgatgcacgc aggcgttcga ccagtgcccc
     8341 cttgccgcgc agcgggcgcg cgccaagctg ctgcagggcg atgcgctggc ggcgcaggct
     8401 caactggtgg gccagcaatt cctccagcgc cagccgccgt tgcgccggat gcaggccggc
     8461 ctgcagcgcg gccacgtcgg cgtcgcgcgg cggccgatgc agggtcacga gggcgtcgcg
     8521 caggcccggc agctgcaagg catcgcgcac ctccagcggc aacagctcca gcgtatcggc
     8581 atccggcaag cgctccagcg caagcccgac cagcttgcgc aggttggccg ggccgatgcc
     8641 ctcgacctcc ggatacaccg gatccagcgc ctcgccgagg ccgtgttcgc cttcgccgag
     8701 gacgcggtag ctgggatgca ccatctccag cccgtgctgg cccggccgcg gggtgccgta
     8761 cgcacgcacc cgcgtgcccg gcgcgaactg cgccgcctgc tgcgcacgga aatggaagaa
     8821 gcgcagcacc agggtggcgc gcgactcgtc ggacagcgcc acccgcagca tcggccggta
     8881 gcggaagcca cgctccaccg cctcgaccct gccttccacc tgcaccgcga cgcccgcctg
     8941 caactgccgg atcggcgcca gcgaggtgcg atcctcgtac tgccgcggca ggtgcagcca
     9001 caggtcctgc agggtcagca ggccgcgtgc ggcgagcttc tccgccagcg ccgggccgac
     9061 gccgggcagc gatgccaacg gggccgcacc ggaggcggca agggtggggc cggcgcgtgc
     9121 gctacgaacc acgaatcagg gcaggaccat catcgcgtcg acctcgaaca ccgcgccctt
     9181 gggcagcgcc ggtacctcga tggtcgagcg cgccgggtac ggtgcggcga agtactcggc
     9241 catcaccgca ttgaccgcgc cgaactgcga caggtcggtg aggtacaggc cgacccgggc
     9301 gatgtcgtcc atgctgccgc cggccgctgc gcagaccgcc tccaggttgt cgaacgcacg
     9361 ccgtgcctgg accgtgatgt cgccctcgac caggttgccg gtggccggat ccagcgggat
     9421 ctggccgctg aggaacacca ggttgccggc gcgggtggcc tgcgaatacg ggccgatcgc
     9481 ggcgggtgca tggtcgctgt ggatgggggt gcggggcatg tcgtcggctc caacgaaagg
     9541 ggatgctgca ttgtaggagt cgcgcaagcc gcgaccgcac ttcccgggga atcggtcgcg
     9601 gcttgcgccg ttcctacagg cgctgcacgc cgtggaccac gttgaggcgg cggatccgcc
     9661 ggatcacgtc cgccaggtgc ttgcggtccg cgacctcgat cgcgaagcgg atcaccgcga
     9721 cccgggtgtc gcgctcgagg tattcgacgc ggtcgatgtt ggattccgca tgtgcgatcg
     9781 cggcggcgac ctgggccagc acgccaggtc ggttctcgac ctcgatccgc agcgcggcgt
     9841 cgaaatcccc gctgacctgg cggtcccagc cgatctgcac ccaacgctcc ggcgacttgc
     9901 ggtactcacc gacgttcggg cactccaggc ggtgcaccac gatgcccttg ccggccgtgt
     9961 ggtagcccat gatctcgtcg cccgggatcg gcatgcagca ttgcgcgaag ctcaccaccc
    10021 cgcgctcggc gccggtgatc aggatccgtt cctgcggcac cgcctgcgcg gaacgcagca
    10081 ggtcgggctc ctgttccggc gttgccagcg cctgcgccac ctgcgccggc atgcggttgc
    10141 cgagggcgat gtcggccagc agcgcctcca gccgcggaaa gcggctctcg gcgaggtagg
    10201 cgtccagcag gatcggtggc agccggtcga gcgaggtgcc cagggtgccc aacgcgcggt
    10261 ccagcatgcg atggccaagc tgcaccgcgt cctcgtgctc gaggtgcttg agctggtggc
    10321 ggatcgcggt gcgggccttg ccggacacca cgaattccag ccactgcggc gccggtgcgg
    10381 cggacttcgc ggtgatgatt tcaacgctct ggccgctggc cagcttggcg cgcagcggcg
    10441 ccagcttcct gtccacccgc gccgccaccg cgtggttgcc gacgtcggta tgcaccgcgt
    10501 aggcgaaatc cagcgcggtg gcgttgcgcg gcagggccag gatgtcgccc ttcggggtga
    10561 acaggtagac ctcgtccggg aacaggtcga ccttcacgtt ctccaggaat tccagcgacg
    10621 agccggtgcc gcgctggctc tccaccaggt tggcgatcca cgccgacgcc cgcccctgcg
    10681 cgctgttgcc ggggtcctcg ccgtgcttgt aggcccagtg cgctgcgacc ccgcgctcgg
    10741 cgaccaggtc catctcgcgg gtgcggatct gcacctcgat cggcgagccg tagggcccga
    10801 acagcaccgt gtgcagcgac tggtagccgt tcgccttggg gatcgcgatg aaatcgcgga
    10861 agcgcccgtc cagcggcttg tacaccgaat gcaccacccc cagcgcgtgg tagcactccg
    10921 ccaccgagtt ggccaccacc cggaacccga acacgtccat cacctgggcg aagctgcggt
    10981 gctcggcgcg catcttggtg tagatgctcc acggcgtctt gatgcgcccg accaggcgat
    11041 gctcgacctt ctccatcgcc agcttctgcg ccagctgcgc ctcgatcttc gccagcgcct
    11101 cgcgccgcac cagcggctgg ctgcggatgc gcttttcgat caccgcgtgc cgccacggat
    11161 gcagcgcgcg gaaccccagg tcctgcagct cggacttgat caggttcatg cccagccgct
    11221 gggcgatcgg cgcgtagatg tcgagggtct cgctggcgat gcggcggcgc gagtcgaccg
    11281 actgcgcgcc cagcgtacgc atgttgtgca ggcggtccgc cagcttgatc aggatcaccc
    11341 gcaggtcgcg cgacatcgcc agcagcatct tgcggaagct ctcggccgcc gcttcctggc
    11401 gatcggcgaa gtgcagcttg tccagcttgg tgacgccatc gaccagcccg gccacggtct
    11461 cgccgaactg ggtggcgatg tcggcccgcg acagcggggt gtcctcgatg gtgtcgtgca
    11521 ggatcgccgc gaccagggtc tcgacgtcga ggccctgctc ggccagcacc tgcgccaccg
    11581 ccaccggatg ggtgatgtag ggctcgccgg acttgcgcag ctggccgacg tgggcagagg
    11641 cccccaccgc ccatgcccgc cgcaacagcg cgcgctgcgc ctccggcagg tagtccgccg
    11701 cacgctccag cgcctgcacg tagtccggca ccgccgcgtc ttcaggcgcg ggcggcgttg
    11761 gcagggcaag ggcgggatcg ccgggactca tggcccgagc gtacccgcct ccccggtcgc
    11821 cggcaaaccg ggccgcgcaa acgaaaacgg ccccgcaggg ccgtttccag taccggtcgc
    11881 cgggcgccgg cgggcgatca gtcgtcgccc ttcatgtcgt cgtcgccagc caccacttcg
    11941 gcggcggccc attccagcgc ctcgcgctcc ttgcgctcgc gctccgactt ctcgaccgcg
    12001 tcgatgtagc cgaggtcgat ctcgcgcgcg gcgatctcgc gcagcgcgag cacggtgggc
    12061 ttgtcgttgg cttcgctgtt gtccagcttc ggctccacgc cgccggccag ctggcgcgcg
    12121 cgcttggcgg ccatcatcac gagctcgaag cggttgtcga ccacttccag gcaatcttcg
    12181 acggtaatac gggccatgtc tgtcctgcga cctgttggcc gccgcgtggt tcaagggggc
    12241 gccaagtcta gccgcaggac gcggcaaccg caagccgccg ccttgaaaat cagaaactta
    12301 gcgcgttcag ccgggctggc cggggtcgtc cgccagcagc gaggtgatca gccgggcatg
    12361 ccgcgccacc tgcgcgtcct tgcgtagccg gctggcggtg aagatgctgc acatctcgcc
    12421 gaccgcggtc tcgaagtcct cgttgacgat cacgtagtcg aattcgccgt agtggctcat
    12481 ctcgtcgcgc gcggcggcca ggcgctgggc gatgacctcc tcgctgtcct gcccgcgctt
    12541 gcgcatgcgc gtctccaggg cggcgcgcga gggcggcagg atgaatacgc tgaccgcatc
    12601 cgggatccgc gaacgcacct ggcgcgcacc ctgccagtcg atctccagca gcacgtcgcg
    12661 cccggccatc agctgcggct ccaccgactg ccgtgcggtg cccttccagt cgccgtgcac
    12721 gcgcgcgtgc tcgaagaagt cgccggcggc gaccatcgcc tcgaactcgt cggcgctgat
    12781 gaagtggtag tgctccgcat gccgttcgcc cggccgcggc tgccgcgacg tgaacgagat
    12841 cgacaggctg atgttgtggt cgcgcgcgag caccgcgttg acgatgctgg atttcccggc
    12901 acccgacggg gcggcgacga tgtacagggt tccgcgcatg ggctactcga tgttctggat
    12961 ctgttcgcgg acctggtcga tcaggacctt cagctccacc gcggcattgc tggtgcgcgc
    13021 gtcgaccgac ttgctgccga gggtgttggc ctcgcggttg aattcctgca gcaggaaatc
    13081 caggcgccgg ccgaccggct ccttgccgcc cttgaggatg cggcgcagct cggccaggtg
    13141 gctgtcgagg cggtccagct cctcgtccac gtcgagcttc tgcaaccaca gcaccagctc
    13201 ctgctcgatg cgccccgggt cggcgggctg cgacaggtcg gccaggcggg cctcgagctt
    13261 gtgccgctgg ccggcgcgga tcagcggcac cagggtgcgc acgtcggcgg cgatcccggc
    13321 gatcgccgcg gcgcgttcgc cgatcaccgc ggcgagcttg ccgccctcgc gctcgcgggt
    13381 ggccacgaag tcggcaagca cgacgtcgag caatgccagc acctcgcgct gcagcgcctc
    13441 ctggtcgagg cctcggccct gcagcacgcc cgggtactgc agcagctcgg tcaggctggt
    13501 gcgcagcccg gggaagcgct gggccaggtc gaggttgagc cgcgaaagtt caccgaccag
    13561 ggcctcattg aggtgcaggc cggcatccga ttccggggcg cgcaggcgca tcgccaggtc
    13621 cagtttgccg cgcgagatcc gcgcaccgat gcgctcgcgc aggatcggct cgaacgcgcg
    13681 cagttcctcg ggcaggcgca gccccagttc gaggaagcgg tggttgaccg cacgcaactc
    13741 gcagcccaac accccccatt cggtgctgcg ctcgccggag gcgaaggcgg tcatgctgcg
    13801 gatcatgcgg agtccagggg cacgggaacg cgaatggtaa tctagcgccc cgctgatttc
    13861 cggcatctcc cacggatcgc ccccgatgtc cttctcccgt cccagcggac gcgcgcccga
    13921 gcagatgcgc gcggtttcga tcacccgcgg ctacacccgc cacgccgaag gctcggtgct
    13981 ggtggcgttc ggcgacaccc gggtgctgtg caccgccagc gtcgagaacc gcgtgccagg
    14041 cttcctgcgc ggcaagggcg aaggctggat caccgccgaa tacggcatgt tgccgcgcgc
    14101 cacccacacc cgcggcgacc gcgaggccgc gcgtggcaag cagggcgggc gcacgctcga
    14161 gatccagcgc ctgatcggcc gctcgctgcg tgcctgcgtc gatcgcgccg cgctcggcga
    14221 gcgcaccatc accctcgact gcgacgtcct gcaggccgac ggcggcaccc gcaccgccgc
    14281 catcaccggc gcctatgtcg cgctggtgga cgcggtgcgc tggctgcggg cgaagaagga
    14341 gatcgccagg gacccggtca tcggcgcggt cgcggcggtg tcggccgggg tgtatcgcgg
    14401 cgtgccggtg ctcgacctcg actaccccga ggacagcgac tgcgacaccg acatgaacgt
    14461 ggtgatgaac gacggtggcg gcttcatcga actgcagggc accgccgagg gccatgcgtt
    14521 ccgccgcgac gagctggatg ccctgctggc gctcgccgag cgcggttgcg gcgaattgtt
    14581 cgcggcgcaa cgggcggcgc tcgccacgcc ttgaaactgg tcctcgccag ttccaacgcc
    14641 ggcaagctcg cggagttgcg cgggctgctc ggcgacggct tcgagctgca cgtgcaatcc
    14701 gaattcgggg tcgccgatgc cgcggaaacc ggcctgtcgt tcgtcgagaa cgcgatcctc
    14761 aaggcccgcc atgcggcgcg cgcgaccggc atgccggcgc tcggcgacga ttccggcctg
    14821 tgcgtggacg ccctgcatgg cgcgcccggc ctgtattccg cgcgctatgc cggggcgcat
    14881 ggcaacgacg aagcgaatat cgacaaattg ctgcatgaac tggatggcgt cgatgacgcg
    14941 cgacgcggcg cccgtttcgt ctgcgtgctg gcactggtgc gcgatgccca cgatccgcaa
    15001 ccgctgctcg ccgagggcac ctggcacggc cggatcctgc gcgaacgccg cggcgacaac
    15061 ggcttcggct acgacccggt gttcttctcg ccggcgcatg gcagcagcgc cgcggagcta
    15121 ccggcgacga tcaaggggcg cgacagccat cgcggcctgg cactggcgaa attgcgcgcg
    15181 ttgctgctgg cgtgatgctc gccacgccgc cactgtcgct gtacgtgcac ctgccgtggt
    15241 gcgtgcgcaa gtgcccgtac tgcgatttca actcgc
//
LOCUS       DAORUK010000041        15088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_3736_length_15088_cov_17.203353, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000041 DAORUK010000000
VERSION     DAORUK010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15088
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3736_length_15088_cov_17.203353"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PK818_04325"
     CDS             <1..642
                     /locus_tag="PK818_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014090027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HQY81995.1"
                     /translation="GVEVCDRIHAVEAFDRPAGAFVRFAAADCAFAYRESLFKRDPGR
                     YIVTAVEFALSRTPALKLDYAGIGEELQAMGIDGTPRASQVAEAVIRIRRRKLPDPAL
                     LGNAGSFFKNPMVAAAVAAGLQAGHPGMPVFRAGDADTRKLSAAWLIDQCGWKGHRDG
                     DAGVAESHALVLVNHGHATGAQLLDLARRIAASVRERFGVAIEPEPRIIGARW"
     gene            651..1568
                     /locus_tag="PK818_04330"
     CDS             651..1568
                     /locus_tag="PK818_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQY81996.1"
                     /translation="MRAALLMLASTVGFGLMAVCIRLASQTEPVAEIAFFRNLFGLAG
                     LLPVLAWPALRGGGAAAHLRQVLRTTQLRRYFIRCLIGMVSMWMGFWAIANLPLAQAV
                     SLAYSSPIFVTIAAVLLLGERVRVRRWLAVVAGFIGVLVIVRPWSHAFSTGSLVAVGA
                     AVITAIVAIQIKQLSHHDGSDTIVLWTYLFWIPMSLPAALWFWHPPQGIAWLWLLLSG
                     VLGTVGQLLWTRALKLGEVSALTPISFVQLVIVTIAGWLWFDERVDRWTALGAAIIFA
                     ATAYIAHREAVLARQHRSNAPSEAVEPGN"
     gene            1614..2513
                     /locus_tag="PK818_04335"
     CDS             1614..2513
                     /locus_tag="PK818_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQY81997.1"
                     /translation="MASSQPPHDTAQHTILSPGPARPQARAARVVVIHGEGLGQRVVV
                     EAAPVLVGRSQEADLVIAHKSVSREHCRIWRDDSGYRVRDLGATNTTRVNDRRIDGEA
                     GLADGDQLTVGESILKFISEDSVEASYHEQIYQLATHDALTELYNRRHFIEAADKEIA
                     RALRQPLPLALCIVDVDLFKPVNDRHGHLSGDEVLRQIAAVLRRHARAGDIAARIGGE
                     EFALLLPGCALDAASTFADRLREAVAATAFAPGGEPQRITVSIGIAALGPGRQDRAAL
                     MAAADAALYRAKHAGRNQVCVQE"
     gene            complement(2550..4508)
                     /locus_tag="PK818_04340"
     CDS             complement(2550..4508)
                     /locus_tag="PK818_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HQY81998.1"
                     /translation="MGRASFPLHLHITLLAVALVAATGLVLRQFGEVIPGPGEFGLLL
                     VAVAVVALLASRRIAAPLAELAEQAEAVRRFDFGDHPLVRSPVREIDQLGGAFDLMRD
                     AVRRFLRMNRRLATETDLEALQPWLLDKMVDLAGARGGVLYLVDAATDALVATAMDAE
                     GGKARANPGMPPLPQAGLPRLMADARATMQPANGHLRDGELEAIGLDPGFAGFGTLAL
                     PLRDRREQLLGMLLLFKDGEIDAANARFIEALAASAATSLETRELLRGQKALLEATIR
                     MVAGAIDAQSPYTGGHCERVPELTFMLARAACAAREGPYAGFDLDPEEWEALHIGAWL
                     HDCGKVTTPEHVVDKATKLETVYDRIHEIRTRFEVLKRDADIAYWRGRAEGGDEAALG
                     DQRDALKAMLDEEFAFVAECNVGGEYLSPEKQRRLRDIGARRWLRTLDDRLGLSRDEL
                     ARHPGASAALPVEEPLLADKPQHRIARPAEEAIPEDNRWGFRMDVPELLYNRGELHNL
                     SVSRGTLTAEERYKINEHMVLTIRMLGELPFPRHLRAVPEIAGGHHEKLDGTGYPRRL
                     DREQLSPLARMMAIADIFEALTAADRPYKPGKTLSQALAIMANMRKDNHIDPELFELF
                     LRTGVHRDYAGRFMLPEQIDEVRIEDYL"
     gene            complement(4608..5867)
                     /gene="ispG"
                     /locus_tag="PK818_04345"
                     /gene_synonym="gcpE"
     CDS             complement(4608..5867)
                     /gene="ispG"
                     /locus_tag="PK818_04345"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HQY81999.1"
                     /translation="MTTPAPDRFGPVPRRETRQVVVGGVKVGGDAPVVVQSMTNTDTA
                     DVAATTRQVADLWRAGSELVRVTVNNAEAAAAVPRVVEKLDMMGIRVPIIGDFHYNGH
                     TLLANEPACAEALAKYRINPGNVGFGKKKDTQFAQLIEFAIRHGKPVRIGANWGSLDQ
                     ALAARLMDENAQRAEPWDAGRVMREALILSALESAQRAVELGLPAEKIILSAKVSGVQ
                     ELVAVYRDLSRRSDFALHLGLTEAGIGSKGIVASSAALGVLLQEGIGDTIRISLTPEP
                     GASRTQEVIVAQELLQTMGLRAFTPMVTACPGCGRTTSEFFQELAGVVQNHVRAKMPE
                     WKISHPGAENMTLAVMGCVVNGPGESRHANIGISLPGTGEAPAAPVFVDGERSVTLRG
                     AGIATEFIALIDAYVEKKYARGIAGGA"
     gene            5896..7230
                     /gene="xseA"
                     /locus_tag="PK818_04350"
     CDS             5896..7230
                     /gene="xseA"
                     /locus_tag="PK818_04350"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQY82000.1"
                     /translation="MADHVLTPSQLNTLARDLLEGAFPLVLVEGEIGNLSRPGSGHLY
                     FTLKDARAQVRCALFKPKSQWLKFVPREGLKVLARGRLTLYEPRGDYQLVLDTMEEAG
                     EGALRRAFDELKAKLAGEGLFDAGRKRALPAFVRRLAVLTSPSGAVIRDIVSVLRRRF
                     PLLEVELLPVPVQGEGAAAQLRAMLQRADASGRYDAILLARGGGSLEDLWAFNDEALA
                     RAIAASNTPVVSAIGHETDFSLSDFAADLRAPTPSAAAELLVPDAASLGLRVDGLQRQ
                     LRATHGNTMRQRAQRVDHAFLRLQARRPQARLDALAARAAQARARLANAIQRRLERDN
                     AALRHADAILRACHPQRRLLQARARLQAIRGRPQALLAQRLQHATLQLRGLVRSLHAI
                     SPLATVARGYSILQHADGRIVRSTGDAAAGERLEARLHHGRLLLRVEGKQDS"
     gene            7233..8657
                     /locus_tag="PK818_04355"
     CDS             7233..8657
                     /locus_tag="PK818_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQY82001.1"
                     /translation="MKTHWPYYLANRPVRANLRLDVLDKFSGERVARVAFADAAAVDA
                     AIAAAHGARAAMAGFPPDARRDVLEHCVRRFGERREELALALCIEAGKPIHDARGEVA
                     RLVDTFRIAAAEATRIGGEVLELGVSARTRGWRGMTKRVAVGACSFITPFNFPLNLVA
                     HKVAPAIAAGCPFVLKPAATTPLGALLIGEVLAETDLPRGAFSILPCSNQVAAALVED
                     ERIALLSFTGGLVGWDLKARAGRKRVTLELGGNAACIVDEHPGASLDQVVERLVFGAY
                     YQGGQSCISVQRILVHRSVYPALEKKLVAAVKELRSGDPRDEATFIGPLIDEAAALRI
                     DGWIDDAVEGGAKVLVRGKRQRNLLPAALLAGVPRTADLYRKEAFGPVACLEAFDDFD
                     EAIARANDSDFGLQAGVFTADIGRAMQAWDGLEVGAVVIGDVPSVRVDNMPYGGIKQS
                     GLGREGVRWAIQDMTEERLLVVRG"
     gene            complement(8654..>11080)
                     /locus_tag="PK818_04360"
     CDS             complement(8654..>11080)
                     /locus_tag="PK818_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQY82002.1"
                     /translation="GSLDMQDMLRRIHAIVGELMYAENFYIVLYDESRQKVRFLYFVD
                     KLDPYVNDPDAELDFDQAEATLTVTLLRGGVALRGPSRDLREQCGIPYLESNGPDSAD
                     WMGVPMLREDRVAGGIVVQSYERAGVYSEEERVLLAYVAQHILTALDRHHAREELEHR
                     VEERTRELRKANLDLQAEVVERQRSQELQRALFRIAELSMTSETLERFYAEIHKVVGG
                     LLYARNFYIAMLSADGSMLEFPYSVDERDAVRRPRRVAKGLTEYVLRTGKALLADRPR
                     IAQLEAAGKVRSIGTLAYCWLGVPLLHDDQVVGVIAVQSYSSDIGFSGRDLELLTFVA
                     YHIGSGLARKQAQDKLVVAHAGLEQRVSERTRELAEANAELVEQMGERLRAERKLTYQ
                     ASHDALTGLPNRVQLLERLARTIAAARRDPNACFAVLFLDLDRFKLVNDSVGHAVGDE
                     LLVEAGRRIVGTVRGTDMVSRLGGDEYAILAEGLDGPAMAEELGLRVLSALGAPIWIA
                     GRELFPTASIGIAMWHPRYDTGEEMLRDADAAMYRAKGTGRDRCTLFDEEMREEATRT
                     LDLEADLRRAIHSDAFEPHYQSIVRIGDAAVVGHEALLRWRHDKHGLLAPGAFLDVGE
                     DSGLIEQVDWLMYARVIADMARTDMPGYVAINVSPRHFRSPDFAYRLIDLLEEAEVSP
                     SRLRIEITEVALLDDAPRISETLDMLRERGVLAQLDDFGTGFSALSYLHRFPIAALKV
                     DRSFVADLDGEARTESVAVIRAITALAGSLGIELIAEGVETESQRERLIELGCVYAQG
                     FLFGRPEPLP"
     assembly_gap    11081..11180
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11223..11774
                     /locus_tag="PK818_04365"
     CDS             11223..11774
                     /locus_tag="PK818_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQY82003.1"
                     /translation="MDGPDASDEALMLAYAAGEATAFEQLYARHRGKLYRYLLRQLRD
                     NGLADELFQDIWQRVVSARTGWKPDAGFATWLYTIAHHRLGDHWRSLKHRPAAPADAD
                     ERTARLADPDTPERVLSEFERRRQLQLALDDLPGEQREVLLLRLEQELTLEEIGEVTG
                     VGRETVKSRLRYAMDKLRAVMPA"
     gene            11771..12637
                     /locus_tag="PK818_04370"
     CDS             11771..12637
                     /locus_tag="PK818_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82004.1"
                     /translation="MTRPPSQRLDADERALAALLPRPHGRVEPGPELDARILAAARAA
                     TQPQPANGPRRRWIGPAALAASLVLAVGLAWQMRAPPAADAPVQATDAASADPAATPM
                     QAFEAPPASPALEDAPPEHVVAQTRRAAATVAATHAAAPPAAADTAAVAPPPPPAPAA
                     PAAASVAGIAAMPQAAEAARAQPQAQAKAGAGDPIRERAAASANAAAPARDQAGNAAA
                     ETADGLGIDDPGEDVPPATADSPAVRDAWLRRIGELLAEGKADDAKASLAEFRRRYPE
                     ATLPPELRTLEP"
     gene            12669..14429
                     /locus_tag="PK818_04375"
     CDS             12669..14429
                     /locus_tag="PK818_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQY82005.1"
                     /translation="MNSPAPAPSKAPIGSLRALWPFVQRHLGLFTGWLLALACSSAAT
                     LSLPVAFRTMIDDGFSSGANIDRTFGFVMVVVLVLALATAVRYFFVTLLGERVVADLR
                     GRLYARLLDLDASFYDRSRTGELVSRLTTDAELLRSVVGSSMSVALRSTVTVFGSLAM
                     LFVTSPKLAAWAVVGIPLAVLPIVLGGRRIQKVSRDSQDRIADANALATEALGAVRTV
                     QAHAREPYERNRYGAALATAVETARRRIGAQAVVTAVAIALVFGAVIVVLWSGAHDVI
                     AGRMTAGTLGQFVMYALIGGGSVGALAEVWNELQRAAGGMGRINDLLHESPGIVAPAQ
                     PRPLPAPLHGEIVFEAVTFHYPQRPDAAALEGFELRVRPGETVALVGPSGAGKSTVFS
                     MLLRFHDPQSGRISLDGIDLRELDPARLRDAIALVPQAPTIFAASAADNIRYGRLEAS
                     DADVRKAAESAEADEFLDALPQGFASELGERGARLSGGQQQRVAIARALLKDAPVLLL
                     DEATSALDAQSERAVQHALENLMAGRTTLVVAHRLATVLKADRIVVMDAGRIVAEGTH
                     AQLLAQGGLYADLAKLQFLD"
     gene            complement(14502..14708)
                     /locus_tag="PK818_04380"
     CDS             complement(14502..14708)
                     /locus_tag="PK818_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HQY82006.1"
                     /translation="MQYGTVKWFNDAKGFGFISPEDGSADLFVHFSAIDSKGFRSLQE
                     GQRVSFEVTQGAKGAQATGVKPVA"
     gene            complement(14848..>15088)
                     /locus_tag="PK818_04385"
     CDS             complement(14848..>15088)
                     /locus_tag="PK818_04385"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399364.1"
                     /note="Catalyzes the phosphorolytic cleavage of
                     6-oxopurine nucleosides; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HQY82007.1"
                     /translation="AEIARMRRDGCDLVGMTGMPEAGLARELGLDYACLAIVANWAAG
                     AGPDVDEVITLQDVLDNVAAAMDGVPRLLRAMLAG"
BASE COUNT         2125 a         5439 c         5210 g         2214 t
ORIGIN      
        1 ggcgtggagg tatgcgatcg catccacgcg gtggaggcgt tcgaccgccc agccggcgcg
       61 ttcgtgcgct tcgccgccgc cgattgcgcc ttcgcctatc gcgaatcgct gttcaagcgc
      121 gatcccgggc ggtacatcgt caccgcggtg gagttcgcgc tgtcgcgcac gccggccctg
      181 aaactcgact acgccggcat cggcgaagaa ttgcaggcga tgggaatcga cggcacgccg
      241 cgcgcgtcgc aggtggccga ggcggtgatc cgcatccgcc ggcgcaagct gccggacccc
      301 gcgctgctgg gcaatgccgg cagcttcttc aagaacccga tggtggcggc ggcggtcgcg
      361 gcagggctgc aggccggcca tcccggcatg ccggtgttcc gcgccggcga cgccgacacc
      421 cgcaagctgt ccgcggcctg gctgatcgac cagtgcggct ggaagggcca ccgcgacggc
      481 gatgccggcg tcgccgaatc gcacgcgctg gtgctggtca accacggcca tgccaccggc
      541 gcgcaactgc tcgacctggc ccggcggatc gccgcctcgg tgcgggagcg cttcggggtc
      601 gcgatcgaac ccgaaccacg catcatcggc gcacgctggt gaacctgcag ctgcgggccg
      661 cgctgctgat gctggccagc acggtcgggt tcggcctgat ggcggtctgc atccggctgg
      721 catcgcagac cgagccggtg gcggagatcg cgttcttccg caacctgttc gggctggccg
      781 gactgctgcc ggtgctggcg tggccggcgc tgcgtggcgg cggcgccgcg gcgcacctgc
      841 gccaggtgct gcgcacgacc cagctgcggc gctacttcat ccgctgcctg atcggcatgg
      901 tcagcatgtg gatggggttc tgggcgatcg ccaacctgcc gctggcgcag gcggtgtcgc
      961 tggcctattc ctcgccgatc ttcgtcacca tcgccgcggt gctgctgctg ggcgaacgcg
     1021 tgcgcgtgcg ccgctggctg gcggtggtgg ccgggttcat cggcgtgctg gtgatcgtgc
     1081 gcccgtggtc gcatgcgttc agcaccggca gcctggtggc ggtgggcgcg gcggtgatca
     1141 ccgcgatcgt ggcgatccag atcaagcagc tttcccacca cgacggttcc gacaccatcg
     1201 tgctgtggac ctacctgttc tggatcccga tgtcgctgcc ggctgcactg tggttctggc
     1261 atccgccaca gggcatcgcc tggctgtggc tgctgctgtc cggggtgctc ggcaccgtgg
     1321 gccagttgct gtggacgcgc gcgctgaagc tgggcgaggt ctcggcgctg accccgatca
     1381 gcttcgtgca gctggtgatc gtcaccatcg ccggctggct gtggttcgac gagcgcgtcg
     1441 accgctggac cgcgctcggt gccgccatca tcttcgccgc caccgcctac atcgcccacc
     1501 gcgaggcggt gctggcgcgc cagcaccgca gcaacgcccc cagcgaggcg gtggaacccg
     1561 gcaactgatc cggctcccgg ctacactcgc ggcgacacca ccgataccct cgcatggcca
     1621 gcagccagcc cccgcacgat accgcccagc acaccatcct cagccccgga ccggcgcgcc
     1681 cgcaggcgcg cgcggcgcgg gtggtggtga tccacggcga aggcctgggc cagcgggtgg
     1741 tggtggaagc cgcgccggtg ctggtcgggc gctcgcagga ggccgacctg gtgatcgcgc
     1801 acaagagcgt ctcgcgcgag cactgccgga tctggcgcga cgattccggc taccgcgtcc
     1861 gcgaccttgg cgccaccaac accacgaggg tcaacgaccg ccggatcgac ggcgaggccg
     1921 ggctggccga cggcgaccag ctgacggtcg gcgagagcat cctcaagttc atcagcgagg
     1981 acagcgtcga ggccagctac cacgaacaga tctaccagct ggccacccac gacgccctga
     2041 ccgagctgta caaccgccgc cacttcatcg aggcggcgga caaggaaatc gcccgcgcgc
     2101 tgcggcagcc tttgccgctg gccctgtgca tcgtcgacgt cgacctgttc aagccggtca
     2161 acgaccgcca cggtcacctg agcggcgacg aggtgctgcg ccagatcgcc gcggtgctgc
     2221 gccggcatgc gcgcgccggc gacatcgccg cgcgcatcgg cggcgaggaa ttcgcgctgc
     2281 tgctgccggg gtgcgcgctg gacgcggcga gcaccttcgc cgaccgcctg cgcgaggcag
     2341 tcgccgccac tgcgttcgcc cccggcggcg aaccgcagcg gatcacggtc agcatcggga
     2401 tcgcggcgct gggacccggg cgccaggacc gggccgcgct gatggccgcg gccgatgccg
     2461 cgctctaccg ggccaagcac gccggccgca accaggtgtg cgtgcaggag tgagcgcgcc
     2521 gcgcgggcgc cgtgtgcgac ggccgcggcc tacaggtagt cctcgatcct gacctcgtcg
     2581 atctgctccg gcagcatgaa gcggccggcg tagtcgcggt gcacgccggt gcgcaggaac
     2641 agttcgaaca actccgggtc gatgtggttg tccttgcgca tgttcgccat gatcgccagt
     2701 gcctgcgaca gcgtcttgcc cggcttgtac gggcggtcgg cggcggtcag cgcctcgaag
     2761 atgtcggcga tcgccatcat ccgcgccagc gggctgagct gttcgcggtc cagccggcgc
     2821 ggatagccgg tgccgtccag tttctcgtgg tggccaccgg cgatctcggg caccgcgcgc
     2881 aggtggcgcg ggaacggcag ctcgcccagc atgcggatgg tcagcaccat gtgctcgttg
     2941 atcttgtagc gttcctcggc ggtcagggtg ccgcggctca ccgacaggtt gtgcagttcg
     3001 ccgcggttgt acagcagttc gggcacgtcc atccggaacc cccagcggtt gtcctccggg
     3061 atcgcctcct ccgccgggcg cgcgatccgg tgctgcggct tgtccgccag cagcggctcc
     3121 tccaccggca acgcagccga ggccccggga tggcgcgcga gctcgtcgcg cgatagcccg
     3181 aggcgatcgt ccagggtccg caaccagcgg cgcgcgccga tgtcgcgcag gcgccgctgt
     3241 ttctccggcg acaggtattc gccaccgacg ttgcactcgg cgacgaacgc gaactcctca
     3301 tccagcatcg ccttcagcgc gtcccgctgg tcgcccaacg cggcctcgtc gccaccctcg
     3361 gcgcggccgc gccagtaggc gatgtcggca tcgcgcttga gcacctcgaa gcgggtgcgg
     3421 atctcgtgga tgcggtcgta caccgtctcc agcttggtcg ccttgtccac cacgtgctcg
     3481 ggcgtggtca ccttgccgca gtcgtgcagc caggcgccga tgtgcagggc ttcccattcc
     3541 tcggggtcga ggtcgaagcc ggcatacggg ccctcgcgcg cggcgcaggc ggcgcgcgcc
     3601 agcatgaagg tcagttcggg cacccgctcg caatggccgc cggtgtacgg actctgggca
     3661 tcgatggcgc cggcgaccat gcggatcgtc gcttccagca gcgccttctg cccgcgcagg
     3721 agttcgcggg tctccagcga ggtggccgcg cttgccgcca gcgcctcgat gaagcgggca
     3781 ttggcggcgt cgatctcgcc gtccttgaac agcagcagca tccccagcaa ctgttcgcgg
     3841 cgatcgcgca gcggcaacgc cagcgtgccg aaccccgcga accccggatc caggccgatc
     3901 gcctccagtt ccccgtcccg caagtgcccg ttcgcaggct gcatggtcgc gcgcgcatcc
     3961 gccatcaggc gcggcagccc ggcctgcggc aacggcggca tgcccgggtt tgcgcgcgcc
     4021 ttgccgcctt cggcatccat cgcggtcgcg accagcgcgt cggtggcggc atcgaccagg
     4081 tacagcacgc cgccacgcgc accggcgagg tccaccatct tgtccagcag ccacggctgc
     4141 agggcctcca ggtcggtctc ggtggccagc cggcggttca tgcgcaggaa ccggcgcact
     4201 gcatcgcgca tcaggtcgaa tgcgccgccg agctggtcga tctcgcgcac cggcgaacgc
     4261 accagcgggt ggtcgccgaa atcgaatcgg cgcacggcct cggcctgttc cgccagttcc
     4321 gccagcggcg ctgcgatccg gcgcgacgcc agcagcgcga ccaccgccac cgcgaccagc
     4381 agcaggccga attcaccggg gccggggatt acctcgccga actgccgcaa caccagcccg
     4441 gtggccgcca ccagggccac cgccagcagg gtgatgtgca ggtgcaacgg aaaactcgcg
     4501 cgacccatgc cgaagacgcc ccctcgcccg gtgccgtcgc catcctagcc gagccgcgca
     4561 tcccccgggt gggttgggcg tgcgccgatg cccgtggcgc cccctcttca ggcgccgccg
     4621 gcgatgcccc gcgcgtactt cttctcgacg taggcatcga tcagggcgat gaactcggtg
     4681 gcgatgcccg caccgcgcag ggtgacggat ctttcgccat ccacgaacac cggcgccgcc
     4741 ggcgcctcgc cggtgcccgg cagtgaaatg ccgatgttgg cgtggcgcga ctcgcccggt
     4801 ccgttgacca cgcaacccat caccgccagg gtcatgtttt ccgcgcccgg atggctgatc
     4861 ttccactccg gcatcttcgc gcgcacatgg ttctggacca cgcccgccag ttcctggaag
     4921 aactccgagg tggtgcgtcc gcaaccgggg caggccgtca ccatcggcgt gaacgcgcgc
     4981 aggcccatgg tctgcagcag ttcctgcgcc acgatcactt cctgcgtgcg cgacgcgccc
     5041 ggctcggggg tcagcgaaat gcggatggtg tcgccgatgc cctcctgcaa cagcacgccc
     5101 agcgccgcgc tggaggccac gatgcccttg ctgccgatgc cggcctcggt caggcccagg
     5161 tgcagggcga agtcgctgcg ccgggacaga tcgcggtaga ccgccaccag ctcctgcacg
     5221 ccggacacct tggcgctgag gatgatcttc tccgccggca ggcccagctc caccgcccgc
     5281 tgcgccgatt ccagcgcgga caggatcagc gcctcgcgca tcacccggcc ggcatcccac
     5341 ggttcggcgc gctgcgcgtt ctcgtccatc agccgcgccg ccagcgcctg gtccagcgaa
     5401 ccccagttgg caccgatccg caccggcttg ccatgacgga tggcgaactc gatgagctgc
     5461 gcgaactggg tgtccttctt cttgccgaaa ccgacgttgc cggggttgat gcggtatttc
     5521 gccagcgctt cggcgcaggc cggctcgttc gccagcaggg tgtggccgtt gtagtggaag
     5581 tcgccgatga tcggcacgcg gatgcccatc atgtcgagct tctcgacgac gcgcggtacc
     5641 gcggcagcgg cttcggcgtt gttcaccgtc acccgcacca gctcggaacc tgcgcgccac
     5701 aggtcggcga cctggcgggt ggtcgcggcg acgtcggcgg tgtcggtatt ggtcatcgac
     5761 tgcaccacca ccggcgcatc gccacccacc ttcaccccgc cgaccaccac ctggcgggtc
     5821 tcgcggcgtg gtaccgggcc gaaacgatct ggggcgggcg tggtcatgca ggcattttag
     5881 cgtcacgatg cacgcgtggc cgaccatgtc ctcaccccca gccagctcaa cacccttgcc
     5941 cgcgacctgc tcgaaggcgc gttcccgctg gtgctggtgg agggcgagat cggcaacctg
     6001 tcgcggcccg gctccggcca cctttacttc accctcaagg acgcccgcgc gcaggtgcgc
     6061 tgcgcgctgt tcaagccgaa gagccagtgg ctgaagttcg tcccgcgcga aggcctgaag
     6121 gtgctcgcgc gcggccgcct gaccctgtac gagccgcgcg gcgactacca gctggtgctg
     6181 gacaccatgg aggaagccgg cgaaggcgca ttgcggcgcg cattcgacga actcaaggcg
     6241 aagctggccg gcgaaggcct gttcgacgcc gggcgcaagc gcgccctgcc cgccttcgtg
     6301 cgccggctcg cggtgctgac ctcgccgtcc ggcgcggtga tccgcgacat cgtgagcgtc
     6361 ctgcgccgac gcttcccgct gctcgaggtc gagctgttgc cggtgccggt gcagggcgaa
     6421 ggcgcggccg cgcaactccg cgcgatgctg cagcgggcgg acgccagcgg acgctacgac
     6481 gcgatcctgc tggcccgcgg cggcggctcg ctggaagacc tgtgggcgtt caacgacgag
     6541 gcgctggcgc gggcgatcgc cgcgtcgaac acgccggtgg tttccgcgat cggccacgaa
     6601 accgatttct cgctcagcga cttcgccgcc gacctgcgcg cacccacgcc ctcggccgcg
     6661 gccgaattgc tggttccgga tgccgccagc ctcggcctgc gcgtggacgg gctgcagcgg
     6721 cagctgcgcg caacccacgg caacacgatg cgccagcgcg cgcagcgcgt cgaccacgcc
     6781 ttcctgcgcc tgcaggcccg gcggccgcag gcgcggctgg acgcgctggc ggcgcgcgcg
     6841 gcgcaggcac gcgcacgcct tgccaacgcg atccagcgcc ggctcgaacg cgacaacgcc
     6901 gcgctgcggc atgccgacgc gatcctgcgt gcctgccatc cgcagcgacg cctgctgcag
     6961 gcacgggcgc ggctgcaggc cattcgcggc aggccgcagg cgctgctcgc gcaacgcctg
     7021 cagcacgcca cgttgcagct gcgagggctg gtgcgttcac tgcatgcgat cagcccgctg
     7081 gccacggtcg cgcgcggcta cagcatcctg cagcacgcgg atgggcgcat cgtgcgcagc
     7141 actggcgatg ccgccgccgg cgagcgactc gaggcacgcc tgcatcacgg ccgcctgctg
     7201 ctgcgggtcg agggcaagca ggacagctga cgatgaagac ccattggccg tactacctcg
     7261 cgaaccggcc cgtgcgggca aacctgcgcc tcgacgtgct cgacaagttc agcggcgaac
     7321 gggtcgcgcg ggtcgccttc gccgatgccg ccgcagtgga tgccgcgatc gccgccgccc
     7381 atggcgcgcg cgcggcgatg gccgggttcc cgccggacgc gcgccgcgac gtgctggagc
     7441 attgcgtgcg ccgcttcggc gaacgccgcg aggaactggc gctggcgctg tgcatcgaag
     7501 ccggcaagcc gatccacgac gcccgcggcg aagtggcgcg gctggtcgat accttccgca
     7561 tcgccgccgc cgaggccacc cgcatcggcg gcgaggtgct ggagctgggt gtttcggcgc
     7621 gcacccgcgg ctggcgcggc atgaccaaac gggtggcagt cggcgcctgc agcttcatca
     7681 ccccgttcaa tttcccgctc aacctggtcg cgcacaaggt ggcgccggcg atcgccgccg
     7741 gctgcccgtt cgtgctcaag ccggcggcga cgaccccgct gggcgcgctg ctgatcggcg
     7801 aggtactggc ggagaccgac ctgccgcgcg gcgcgttctc gatcctgcca tgcagcaacc
     7861 aggtcgccgc ggcgctggtc gaggacgaac gcatcgccct gctcagcttc accggcggcc
     7921 tggtcggctg ggacctgaag gcgcgcgccg ggcgcaagcg ggtgaccctg gaactgggtg
     7981 gcaacgcggc ctgcatcgtc gacgagcacc cgggtgcatc gctggaccag gtggtggagc
     8041 ggctggtctt cggcgcctac taccagggcg ggcagagttg catcagcgtg cagcgcatcc
     8101 tggtccatcg cagcgtgtac cccgcgctgg agaagaagct cgtcgcggcg gtgaaggaac
     8161 tgcgcagtgg cgatccgcgc gacgaggcca ccttcatcgg gccgctgatc gacgaagccg
     8221 ccgcgctgcg catcgatggc tggatcgacg atgcggtgga gggcggcgcg aaggtcctgg
     8281 tgcgcggcaa gcgccagcgc aacctgctgc ccgccgcatt gctcgcaggc gtgccgcgca
     8341 ccgccgacct gtaccgcaag gaggcgttcg gtccggtcgc ctgcctggag gcattcgacg
     8401 acttcgacga ggcgatcgcg cgcgccaacg acagcgactt cggcctgcag gccggcgtgt
     8461 tcaccgccga catcggccgc gcgatgcagg cctgggacgg gctggaagtc ggcgccgtgg
     8521 tgatcggcga cgtgcccagc gtccgtgtcg acaacatgcc ctacggcggc atcaagcagt
     8581 ccggcctcgg ccgcgagggc gtgcgctggg cgatccagga catgaccgag gaacgcctgc
     8641 tggtggtgcg cggctagggc agcggttccg gccttccgaa caggaagccc tgcgcataca
     8701 cgcacccgag ttcgatcagg cgctcgcgct gcgactccgt ttccacgccc tcggcgatca
     8761 gctcgatgcc caggctgccg gccagcgcgg tgatcgcgcg gatcaccgcc acgctctccg
     8821 tgcgcgcctc gccgtcgagg tccgccacga aactgcgatc gaccttgagc gcggcgatcg
     8881 ggaaccggtg caggtacgac agcgccgaga agccggtgcc gaaatcatcc agctgcgcca
     8941 acaccccgcg ttcgcgcagc atgtccaggg tctcgctgat ccgcggcgcg tcgtcgagca
     9001 gcgccacttc ggtgatctcg atgcgcagcc gcgacggcga gacctcggct tcttccagca
     9061 ggtcgatcag ccggtaggcg aaatccggcg agcggaaatg gcgcggggag acgttgatcg
     9121 ccacgtagcc aggcatgtcg gtacgcgcca tgtccgcgat cacccgcgca tacatcagcc
     9181 agtcgacctg ctcgatcaac ccgctgtcct cgccaacgtc caggaacgca cccggcgcca
     9241 gcaggccgtg cttatcgtgg cgccagcgca gcagcgcctc gtggcccacc accgcggcgt
     9301 cgccgatgcg cacgatcgac tggtagtgcg gctcgaacgc gtcgctgtgg atcgcgcgcc
     9361 gcaggtcggc ttcaagatcc agggtgcggg tggcttcctc gcgcatttcc tcgtcgaaca
     9421 gggtgcagcg atcgcggccc gtgcccttgg cgcggtacat cgcggcgtcg gcgtcgcgca
     9481 gcatttcctc gccggtgtcg taacggggat gccacatcgc gatgccgatg ctggcggtcg
     9541 ggaacagctc gcggccggcg atccagatcg gtgcgcccag cgcggaaagc acccgcagcc
     9601 cgagttcctc cgccatcgcc gggccatcca ggccttcggc gaggatcgcg tattcgtcgc
     9661 cgcccaggcg cgagaccatg tcggtgccgc gcacggtgcc gacgatcctg cgccccgcct
     9721 cgaccagcag ttcgtcgccg accgcgtggc caacgctgtc gttgaccagc ttgaaccggt
     9781 ccaggtccag gaacagcacc gcgaagcagg cattggggtc gcgacgcgcg gcggcgatcg
     9841 tgcgcgccag ccgttccagc aactgcaccc ggttggggag gccggtcagc gcgtcgtggc
     9901 tggcctggta ggtcagcttg cgctcggcgc gcaggcgttc gcccatctgc tcgaccagct
     9961 cggcgttggc ctcggccagt tcgcgggtgc gttcgctgac ccgctgctcc agcccggcgt
    10021 gggcgaccac cagcttgtcc tgcgcctgct tgcgggccag cccgctgccg atgtggtagg
    10081 cgacgaaggt gagcagttcg aggtcgcgcc cgctgaagcc gatgtccgac gagtaactct
    10141 gcaccgcgat cacgcccacc acctggtcgt cgtgcagcag cggcaccccg agccagcagt
    10201 acgccagggt accgatgctg cggaccttgc ccgccgcctc cagctgggcg atgcgcggcc
    10261 ggtccgcaag cagcgccttg ccggtgcgca gcacgtattc ggtcaggccc ttggcgaccc
    10321 ggcgcggcct gcgcaccgcg tcgcgctcgt ccaccgaata cgggaattcg agcatgctgc
    10381 cgtccgccga cagcatcgcg atgtagaaat tgcgggcata cagcaggccg cccaccacct
    10441 tgtggatctc ggcatagaag cgctcaagcg tctcgctggt catcgacagt tcggcgatgc
    10501 ggaacagcgc ccgctgcagc tcctgcgaac gctggcgttc caccacctcg gcctgcaggt
    10561 cgaggttggc cttcctcagc tcgcgggtac gctcctcgac gcggtgctcg agctcttcgc
    10621 gcgcgtggtg gcgatccagc gcggtcagga tgtgctgcgc cacgtaggcc agcagcacgc
    10681 gctcctcctc gctgtagacg ccggcgcgtt cgtagctctg gacgacgatg ccgccggcca
    10741 cgcgatcctc gcgcagcatc ggcacgccca tccagtccgc gctgtccggg ccgttgcttt
    10801 ccaggtacgg gatgccgcac tgctcgcgca ggtcgcgcga tggcccgcgc aacgccaccc
    10861 cgccgcgcaa cagggtcacg gtgagggtgg cttcggcctg gtcgaagtcc agctccgcgt
    10921 ccgggtcgtt gacgtacggg tccagcttgt cgacgaaata caggaaccgc accttctgcc
    10981 gcgactcgtc gtacagcacg atgtagaagt tctcggcata catcagctcg ccgacgatcg
    11041 cgtggatccg gcgcagcatg tcctgcatgt cgaggctgcc nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn gcgcccttcc ctgcgccgcc ggcaatccgt aggctggcgc
    11221 cggtggacgg cccggacgcc agcgacgaag ccctgatgct cgcctatgcg gcgggcgagg
    11281 cgacggcgtt cgaacagttg tacgcgcgcc atcgcggcaa gctgtaccgc tacctgctgc
    11341 gccagctgcg cgacaacggg ctggccgatg agctgttcca ggacatctgg caacgggtgg
    11401 tatccgcgcg cacgggctgg aagccggacg ccggattcgc gacctggctg tacaccatcg
    11461 cccaccaccg cctgggcgac cactggcgca gcctcaagca tcgccccgcg gcgccggccg
    11521 acgccgacga gcgcaccgcg cggcttgccg acccggacac gcccgaacgc gtattgtccg
    11581 agttcgagcg ccgccgccag ttgcagctgg cgctggacga cctgcctggt gaacaacgcg
    11641 aggtactgct gttgcggctg gaacaggaac tgacgctgga ggagatcggc gaagtcaccg
    11701 gtgttggccg ggagaccgtg aagtcgcgcc tgcgctacgc gatggacaag ttgcgcgcgg
    11761 tgatgccggc atgacccgcc cgcccagcca acgcctggat gccgacgagc gcgcgctggc
    11821 cgcgctgctg ccgcgcccgc atggccgcgt cgagccaggc ccggaactcg acgcgcgcat
    11881 cctcgcggcg gcgcgggccg ccacccagcc gcagcccgcg aacggaccgc ggcggcgctg
    11941 gatcggaccg gccgcgctgg cggcctcgct ggtgctggcg gtgggcctgg cctggcaaat
    12001 gcgcgcacca ccggcggccg atgccccggt gcaggccacc gatgccgcat ccgcggatcc
    12061 ggcagccacg ccgatgcagg ccttcgaagc gccgcccgca tcccccgcgc tggaggacgc
    12121 cccgccggag cacgtcgtgg cgcagacccg ccgggcggcc gcgaccgttg ccgcgacgca
    12181 cgcggcggca ccgccggcag ccgccgacac cgcggcggtc gcacccccgc ccccgccggc
    12241 acccgccgct cccgccgctg ccagcgttgc cgggatcgcg gccatgccgc aggccgcgga
    12301 agctgcgcgc gcgcaaccgc aggcgcaggc caaggcaggt gccggtgacc cgatccgcga
    12361 gcgcgcggcg gcatccgcca atgccgccgc gcctgcacgc gaccaggccg gcaacgccgc
    12421 cgcggagacg gccgatggcc tcggcatcga cgatcccggc gaggacgtgc cgcctgccac
    12481 cgcggattcg cccgccgtgc gcgacgcctg gctgcgccgc atcggcgagt tgctggcgga
    12541 aggcaaggcc gatgacgcga aggccagcct cgccgaattc aggcgccgct atccggaggc
    12601 gaccctgccg cccgagttgc gcacgctcga gccctgacgc gcggcggccg cgcagctaac
    12661 atgcatccat gaattccccc gcaccggcgc catcgaaggc gcccatcggt tcgctgcgcg
    12721 ccctgtggcc gttcgtgcag cgccacctcg gtctgttcac cggctggctg ctggcgctgg
    12781 cgtgttcgtc cgcggccacc ctcagcctgc cggtcgcgtt ccgcaccatg atcgacgacg
    12841 gcttcagcag cggcgccaac atcgaccgca cgttcggctt cgtgatggtg gtggtgctgg
    12901 tgctggcgct ggcgaccgcg gtgcgctact tcttcgtgac cctgctcggc gagcgggtgg
    12961 tggccgacct gcgcggccgg ctctacgcga gattgctgga cctcgatgcc agcttctacg
    13021 accgcagccg caccggcgaa ctggtttcgc gcctgaccac cgacgccgaa ctgctgcgca
    13081 gcgtggtcgg ctccagcatg tcggtggccc tgcgcagcac cgtcaccgtg ttcggttcgc
    13141 tggcgatgct gttcgtcacc agcccgaagc tggcggcgtg ggcggtggtc gggatcccgc
    13201 tggcggtgtt gccgatcgtg ctcggcggac gccgcatcca gaaagtctcg cgcgacagcc
    13261 aggaccgcat cgccgatgcc aacgcactgg cgaccgaagc gctcggcgcg gtgcgcacgg
    13321 tgcaggcgca cgcgcgcgag ccctacgagc gcaaccgcta cggcgccgcc ctggcgaccg
    13381 cggtggaaac ggcgcgccgg cgcatcggcg cgcaggcggt ggtgaccgcc gtcgccatcg
    13441 cgctggtgtt cggggcggtg atcgtggtgc tgtggtcggg cgcgcacgac gtgatcgccg
    13501 ggcggatgac cgccggtacc ctcggccagt tcgtgatgta cgcgctgatc ggcggcggct
    13561 ccgtgggtgc gctggccgag gtctggaacg agctgcagcg cgccgccggc ggcatgggcc
    13621 ggatcaacga cctgctgcat gagtcaccgg gcatcgtcgc gcccgcgcag ccgcggccac
    13681 tgcccgcgcc gctgcatggc gagatcgtgt tcgaggccgt caccttccac tacccgcaac
    13741 gcccggatgc cgcggcgctg gagggcttcg agctgcgcgt gcggcccggc gaaacggtgg
    13801 ccctggtcgg cccgtcgggt gccggcaaga gcacggtgtt ctcgatgctg ctgcgtttcc
    13861 acgacccgca gtccggccgc atctcgctgg acggcatcga cctgcgcgaa ctggacccgg
    13921 cgcgcctgcg cgacgcgatc gcgctggtgc cgcaggcgcc gaccatcttc gccgccagcg
    13981 cggccgacaa catccgctac ggccgcctcg aggccagcga cgccgatgtg cgcaaggcgg
    14041 cggaatccgc cgaggccgac gagttcctgg atgccctgcc gcagggcttc gccagcgaac
    14101 tcggcgaacg cggcgcgcgc ctgtccggcg gccagcagca acgggtcgcg atcgcgcgcg
    14161 ccctgctcaa ggacgcaccg gtcctgctgc tggacgaggc gacctccgcg ctggatgcgc
    14221 aaagcgagcg cgcggtccag cacgcgctgg agaacctgat ggccggccgc accaccctgg
    14281 tggtcgcgca tcgccttgcg accgtgctca aggccgaccg catcgtggtg atggatgccg
    14341 ggcgcatcgt cgccgaaggg acgcatgcgc agctgctcgc acagggcggg ctgtacgcgg
    14401 acctggcgaa actgcagttc ctggactgac cgcgacgcaa tcccgcgggt gaaaaaaggc
    14461 cccgcgatgc ggggcccgga atgtggcgct gcggccgtgg gtcaggcgac cggcttgacc
    14521 ccggtggctt gcgcaccctt cgcaccctgg gtgacttcga agctgacccg ctggccttcc
    14581 tgcaggctgc ggaagccctt ggagtcgatc gcggagaaat gcacgaacag atccgcgctg
    14641 ccatcctctg gcgagatgaa accgaagccc ttggcgtcgt tgaaccattt cacggtgccg
    14701 tactgcatgg attgaatgac ctcttgcgaa tgtttgctcg aagctggttg gcttgaagcg
    14761 ggttggctgg aacgtgcatc gcgcggggga ccggcggcgc acgccgcgag tatgcaacaa
    14821 ccggattcgc ttgcaacgcc cgcgcgctca gccggccagc atcgcccgca gcaggcgcgg
    14881 caccccgtcc atcgccgcgg ccacgttgtc cagcacgtcc tgcagggtga tcacttcgtc
    14941 cacgtcgggg ccggccccgg ccgcccagtt ggcgacgatg gccaggcagg catagtccag
    15001 ccccagttcg cgcgccagtc cggcctcggg catcccggtc atgccgacca ggtcgcagcc
    15061 gtcgcggcgc atgcgcgcga tctccgcc
//
LOCUS       DAORUK010000042        15041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_3756_length_15041_cov_18.105031, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000042 DAORUK010000000
VERSION     DAORUK010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 15041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15041
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3756_length_15041_cov_18.105031"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /gene="metK"
                     /locus_tag="PK818_04390"
     CDS             complement(<1..931)
                     /gene="metK"
                     /locus_tag="PK818_04390"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HQY82008.1"
                     /translation="MSSYLFTSESVSEGHPDKLADQISDAVLDAILAQDKRARVACET
                     MVKTGAAIVAGEVTTDAWVDIEALARKVINDIGYDNSKVGFDGHTCAIINMLGKQSPD
                     IAAGVDGSKKKQKKPEEQGAGDQGLMFGYACNEAPEFMPAPIYYSHRLVEQQAKVRKN
                     GKLKWLRPDAKSQVTLRYSEAGHQIEGLDAVVLSTQHDPEVKHKDLVEAVREHILKPV
                     LPKKWMDALAKNKVHINPTGIFVIGGPVGDCGLTGRKIIVDTYGGMARHGGGAFSGKD
                     PSKVDRSAAYAARYVAKNIVAAGLADKCEVQVSY"
     gene            1148..2890
                     /locus_tag="PK818_04395"
     CDS             1148..2890
                     /locus_tag="PK818_04395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HQY82009.1"
                     /translation="MIELEQRLQQRFPQWFRGRRAVIARPLVRGLGRWSRLDVIDAFL
                     AANRGLRGFDFVAAALQFLGARYQVDPAALARIPAQGRVLVVANHPSGALDALALLDA
                     VGTVRRDVRIVANDVLSALDNIGELLLPVRILGGRPSADSLRAIDAALARDECVIVFP
                     AGEVSRLGPRGVRDTRWRRGFLRFARGCDAPVLPVRIQARNSALFYGASALFKPAGTA
                     LLVREMFARRARRIVLRIGQPRRIPADADGAALLRDIRRELYALGTRREQAAAPGDAG
                     PEPVAAEVDPALVRAGIAGLRSLGETSDGKQIRVGALAADAPLLREIGRLRELTFRAV
                     GEGTGRSLDLDPWDSWYEHIVLWDAGAARIAGAYRIARGAPVLAAHGIRGLYTASLFD
                     YADDAIARIAQGMELGRSFVAPDYWGSRSIDYLWQGIGAYLRAHPKVRYLFGAVSISA
                     ALPPAAREQIAAHYAHYFGGDAGEVVSKRPFAYRAAQPRDAAVDAETAFRVLKANLDA
                     LGAQVPMLYKQYTELCEPGGARFLAFGVDPDFSDAVDGLIEVDIQRMRPKKRQRYLGE
                     AKPGTTPHAAAEAA"
     gene            2887..3717
                     /locus_tag="PK818_04400"
     CDS             2887..3717
                     /locus_tag="PK818_04400"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HQY82010.1"
                     /translation="MNPAAAPVPLRRAVFISDVHLGSRHCHAAELADFLARLRCKRLY
                     LVGDIVDLWWMAQRRASWGAAQNRVVEALHALRRAGTEIVYIPGNHDRPVRRFCGLAL
                     PAMRVRRRAIHVTADGRRLLVTHGDEFDAITHFGSLQEKFGDWLYYRILGGNQLLNFV
                     RRRLGLRYWSLSEFLKRQSGAAERFIDRFVQAGLDDVRRRGLDGIVCGHIHRAALVQR
                     DGLVYANDGDWVESLSALVEEADGSLRLLAHTGEVLAEVLPRWRPMRSPGVGTLPQAA
                     "
     gene            3750..4814
                     /locus_tag="PK818_04405"
     CDS             3750..4814
                     /locus_tag="PK818_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HQY82011.1"
                     /translation="MDSLTQIVLGGAVAAAIAPAPHRRAALLAGAMLGTLPDLDALPL
                     ALLTDDPVARMTWHRSASHSLFVLPLLGWAIWALFRRSGGRVAESPLRWFWAIQLALV
                     THPLLDAFTVYGTQLWWPLPMRSVMWSSVFIIDPLYTIWLLAACIAAWWLRERIAAQR
                     VLLAGLVLSSAYLAWSLLAKVAVDREAGRALAAIGLADAPRFSVPMPFNTVLWRVVAM
                     TPQGFVEGERSLLADSGPMVFRGYRSNLEALRAAQGIAAVQRLDWFNHGFQKAQVRDG
                     VLVLSDLRMGAEPDYTFNFAVAERDGNAWRAIPPEQLRWPWQATRRLTAMWERIWTQP
                     ALAQAAAGNPVATPAASASK"
     gene            complement(4760..5758)
                     /gene="dusB"
                     /locus_tag="PK818_04410"
     CDS             complement(4760..5758)
                     /gene="dusB"
                     /locus_tag="PK818_04410"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HQY82012.1"
                     /translation="MRIGRHHIESRTILAPMAGVTDKPFRLLCKRLGAGMAVSEMTSS
                     DPRFWRTAKSLHRMDHVGEPDPVSVQIAGTVPAVMAAAARHNVEHGAQLIDINMGCPA
                     KKVCNAWAGSALMREPQLVGRILAAVVESVDVPVTLKIRTGWDASHRNAAEIARIAED
                     AGIAALTVHGRTRDQQYAGSAEYDTIARIKSILRIPVIANGDIDSPRKAKQVLDATGC
                     DAVMVGRAAQGRPWILGQIGHYLATGELVPEPSLRQVRDILLGHLGHLHAFYGEATGV
                     RIARKHLGWYAKDRPENAAFRSVVNSAQTADEQLRLTRDYFDALAAGVATGLPAAA"
     gene            complement(5799..6746)
                     /locus_tag="PK818_04415"
     CDS             complement(5799..6746)
                     /locus_tag="PK818_04415"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011409356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HQY82013.1"
                     /translation="MARVAVVGSFNVDHVWRVAALPQAGATLGGDYASGPGGKGFNQA
                     MAARRAGAEACFVCALGGDLGAQLARALCTADGIDLRDAESAAPTGTAGIFVDASGRN
                     CIVVGAGANATLDAGFVAASLEALGAVSVVLAQLESPADAIARALTHGRARGATTILN
                     PAPANATASGALLELADILTPNETEFAALLGRHVGERIEADAVSSLDQGRLHALCRQL
                     GHGSSVVVTMGASGCFVSHPDGNLRGDEAACYRISADAANAVDTTGAGDAFNGALAAS
                     LALRPRQPFAEHIRFATRYAGLSTECEGAALAMPRLPTA"
     gene            complement(6751..8049)
                     /locus_tag="PK818_04420"
     CDS             complement(6751..8049)
                     /locus_tag="PK818_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HQY82014.1"
                     /translation="MEALGRIGFGLFGLAVLLGIAWLFSSNRRAVDWKLVATGVSLQI
                     LFAALVLLVPGGRDVFDALGRGFVKVLSFVAAGSNFIFGSLMDTSKFGFIFAFQVLPT
                     IIFFASLMGVLYHLGVMQWIVRGMAWAITKVMRVSGAETTSVCASVFIGQTEAPLTVR
                     PYIARMTESELITMMIGGMAHIAGGVLAAYVGMLGGGDPVQQAFYAKHLLAASIMAAP
                     ATLVVAKLLIPETGEPLTRGTVKMEVEKTASNVIDAAAGGAAEGLKLSLNIAAMLLAF
                     IALIAMINAPLTWLGEVSGLSATLGKPTDLAAIFGWVLAPLAWVIGVPWQDASTVGSL
                     IGQKVVINEFVAYLQLSEIVNGNTPGVALTEQGKLIATYALCGFANFSSIAIQIGGIG
                     GLAPERRSDLARFGLRAVLGGSIATFMTATIAGVLSAFGG"
     gene            complement(8119..9267)
                     /locus_tag="PK818_04425"
     CDS             complement(8119..9267)
                     /locus_tag="PK818_04425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQY82015.1"
                     /translation="MGAFETDAVAGLRDFRRRLLARPDALMLELGAGGELLVARLRAL
                     LSMAMLSMPLVNLLAGGKASETMVGLFGVVLAIVLSQAWLALARQRQRHRWLPWATAA
                     YDVSLTTLVLAILAVDSPVTGLNSMVVWAFYLVSIAMTALRNDGRLTLFSGALAIAQY
                     GVLAAAIFALVPNPDQLASIDYGTVTASNQIQRLVLLALMTAITATIVYRMQKLVDMS
                     GTDGLTGLPNRTWLVHRFPVMLDAIRGQGGSLAVCLIDLDYFKRINDEVGHLAGDRAL
                     RHVVGVLRQQMQEGDWLARLGGEEFALLLPLPLGRAWERLEAMRRTVATQPFAPEHGA
                     ELMRLTFSAGIASWPQDGADLSQLLRRADLRLRHAKLEGRNRVLARDP"
     gene            complement(9298..9876)
                     /locus_tag="PK818_04430"
     CDS             complement(9298..9876)
                     /locus_tag="PK818_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HQY82016.1"
                     /translation="MPPPMLDALLVATGTVALAEIGDKTQLLALLLAARFRKPWPIIA
                     GILVATLLNHALAAWMGQLAAGWLTPQMLRWVVAASFIGIALWTLKPDQLDDDERLPL
                     RGAFIATTIAFFIAEMGDKTQVATVLLAAKYASLWQVVAGTTAGMLLANVPVVALGSR
                     FAARLPLKAARIAAACVFLALGAWAALSGVGG"
     gene            9961..10920
                     /locus_tag="PK818_04435"
     CDS             9961..10920
                     /locus_tag="PK818_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQY82017.1"
                     /translation="MQYRRLGTSGLQLSALAFGAWVTFGNQVGRGTARELVAAAWDHG
                     VNFFDNAEGYANGEAEKVMGDVIADLRLPRDGWCVSSKVYFGSAADPRPTQKGLSRKH
                     VHDACHAALKRLRVDYLDLYFCHRPDPQTPIAETVSAMDALIRQGKVLYWGTSEWSAG
                     EIREAHRVAHAHNLVAPTMEQPQYNLLERERVEVEYAPLYAEFGMGTTIFSPLASGLL
                     TGKYNDGIPAGSRLAQDGMGWLQRGLHGDRIEALRRFTSLAAELGVAPATLAIAWCLR
                     NPNVSSVLLGATGLAQLLQDLEALEVWQRLDDASWERVQASLA"
     gene            10994..11947
                     /locus_tag="PK818_04440"
     CDS             10994..11947
                     /locus_tag="PK818_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQY82018.1"
                     /translation="MGPLLALIVLFAGVVILFKTVRMVPQGYEWTVERFGKYTHTMTP
                     GLHFLVPVVYGVGRKINMMEQVMDVPSQDVITKDNAVVKVDGVVYFQVLDAAKAAYEV
                     ATLEIAILNLVMTNIRTSIGSMDLDESLSKRDEINAKVLVAVDQATHPWGLKVNRIEL
                     KDIQPPRDLVDSMARQMKAEREKRANILEAEGFRQAAILKAEGEKQSVILEAEGEKEA
                     AFRQAEARERLAEAEAKATQMVSDAIASGNVQAINYFVAQKYIEAFKALAEGPNQKFV
                     LMPMESAGVIGSLGGIAELAKEAMDRQAGGAKVIPSPKAGG"
     gene            11951..12382
                     /locus_tag="PK818_04445"
     CDS             11951..12382
                     /locus_tag="PK818_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HQY82019.1"
                     /translation="MRWDVFAWAAIALLLFAAEALAPGAFMLWLGFAAVAVFLGVLLV
                     PGIPVLAQAAAFIALGFVSIQVYRKWFRTRERPSDQPALNRRSAALVGRVVPLERAIV
                     DGRGRVQIADAFWEVTGPELPAGTHVRIVGGDAMSLQVEPA"
     gene            12465..13565
                     /gene="gcvT"
                     /locus_tag="PK818_04450"
     CDS             12465..13565
                     /gene="gcvT"
                     /locus_tag="PK818_04450"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HQY82020.1"
                     /translation="MTQKTILNETHRALGAKMVDFGGWDMPIHYGSQLDEHHLVRRDA
                     GMFDVSHMTVVDLTGAKVRDFLRHLVANSVDKLQKPGKALYTAMLNPQGGVIDDLIVY
                     FMSEDWFRLVVNAATREKDVAWITAQATAFAVDVRERPEFGMVAVQGPNAREKVVGLL
                     REEDRAPVSKLLRFSAREARTTDGVEVFVARTGYTGEDGFEVIVPEAQTVAFWNALLE
                     AGVKPAGLGARDTLRLEAGMNLYGQDMDDTVSPFEAALAWTVVLDEGRDFIGRAALEA
                     QQAAGVSRQMIGLVMDEKGVLRHGQKVLTANGEGEILSGTFSPSIGKAIAFARVPAGA
                     PGEVRVDIRGKEVPVRVVKFPFVREGKVQDGI"
     gene            13646..14041
                     /gene="gcvH"
                     /locus_tag="PK818_04455"
     CDS             13646..14041
                     /gene="gcvH"
                     /locus_tag="PK818_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010368686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HQY82021.1"
                     /translation="MSEIPGDLKFLKSHEWARVEGDGKVTVGISDHAQGLLGDLVYVE
                     LPNVGDSVEAGNACAVVESVKAASDVYAPVSGKVVEVNAALSDKPETINEDAYGDGWL
                     FTMVIDDAEQLNELLDPDAYAELLEEEDH"
     gene            14843..>15041
                     /locus_tag="PK818_04460"
     CDS             14843..>15041
                     /locus_tag="PK818_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82022.1"
                     /translation="MAVKKAAAKKAAKKPAAKKAAKKVAKKPAAKKVAKKAAAKKPAA
                     KKAVKKAAAKKPAAKKAVKKAA"
BASE COUNT         2330 a         5133 c         5296 g         2282 t
ORIGIN      
        1 cgtagctgac ctgcacctcg cacttgtcgg ccaggccggc ggcgacgatg ttcttggcca
       61 cgtagcgggc ggcgtaggcg gccgaacggt cgaccttgga cggatccttg ccggagaacg
      121 cgccgccgcc gtggcgggcc atgccgccgt aggtgtcgac gatgatcttg cggccggtca
      181 ggccgcagtc gccgaccggg ccgccgatca cgaagatgcc ggtcgggttg atgtggacct
      241 tgttcttcgc cagcgcgtcc atccacttct tcggcagcac cggcttgagg atgtgctcgc
      301 gcaccgcctc caccaggtcc ttgtgcttga cctcggggtc gtgctgggtg gacagcacca
      361 ccgcgtccag gccctcgatc tggtggccgg cctcgctgta gcgcagggtg acctgcgact
      421 tcgcgtccgg gcgcagccac ttcagcttgc cgttcttgcg caccttggcc tgctgctcga
      481 caaggcggtg gctgtagtag atcggcgcgg gcatgaattc cggcgcctcg ttgcaggcgt
      541 agccgaacat caggccctgg tcgccggcgc cctgttcctc cggcttcttc tgcttcttct
      601 tgctgccgtc cacgccggcg gcgatgtccg gcgactgctt gcccagcatg ttgatgatgg
      661 cgcaggtgtg gccgtcgaag ccgaccttgg aattgtcgta gccgatgtcg ttgatcacct
      721 tgcgcgccag cgcctcgatg tcgacccagg cgtcggtggt cacctcgccc gcgacgatcg
      781 cggcgccggt cttgaccatg gtttcgcagg cgacgcgggc acgcttgtcc tgggccagga
      841 tcgcgtccag cacggcgtcg gagatctggt cggcaagctt gtccgggtgg ccttcggaca
      901 ccgattcgga ggtgaacagg tagctggaca tcggggctcc tagaaaatga ttcatccttc
      961 aatctggatg aaaagatggc cgcgcatgat acatgggtgc gggcaggcgt gcatcttgcg
     1021 gcatccgcgg cccgctgtgt gcggcacgcg cgatgcccgg cggcggtcac gccaccgcaa
     1081 cctcgcggca atggcgcggt cgcatcgggc ccgcaggatg cccgccaaga ccggcattgc
     1141 gtccgccatg atcgagcttg aacaacgcct gcagcagcga ttcccgcagt ggttccgcgg
     1201 ccgtcgcgcc gtgatcgcgc ggccgctggt gcgcgggctg gggcgctggt cgcggctcga
     1261 cgtgatcgac gccttcctcg ccgccaaccg cggcctgcgc ggcttcgatt tcgtcgcggc
     1321 ggcgctgcag ttcctcggcg cgcgctacca ggtcgatccg gccgcgctgg cgcggatccc
     1381 cgcccagggg cgggtgctgg tggtcgccaa ccatcccagc ggcgcgctgg atgcattggc
     1441 gctgctggat gcggtgggca cggtccggcg cgacgtgcgg atcgtcgcca acgatgtcct
     1501 gtccgcgctg gacaacatcg gcgagctgct gctgccggtg cgcatcctcg gcggccggcc
     1561 aagcgcggac agcctgcgcg cgatcgacgc cgcgctggcc cgggacgagt gcgtgatcgt
     1621 gttcccggcc ggcgaggtct cgcgcctggg cccgcgcggc gtgcgcgata cccgctggcg
     1681 gcgcgggttc ctgcgtttcg cccgcggctg cgacgcgccg gtgctgccgg tgcgcatcca
     1741 ggcccgcaat tcggcgctgt tctacggcgc ctccgcgctg ttcaagccgg cgggcacggc
     1801 gctgctggtg cgcgagatgt tcgcccgccg tgcgcgccgg atcgtgctgc ggatcggcca
     1861 gccacggcgg atcccggccg atgccgatgg cgcggcgctg ctgcgcgaca tccgccgcga
     1921 gctgtatgcg cttggcaccc gccgcgagca ggccgcagcc cccggcgatg ccggtcccga
     1981 gccggtcgcc gccgaggtcg acccggcgct ggtgcgcgcc ggcatcgccg ggctgcgttc
     2041 gctgggcgaa accagcgatg gcaagcagat ccgggtcggc gccctggcgg cggacgcgcc
     2101 gctgctgcgc gagatcggcc gcctgcgcga actcaccttc cgcgcggtgg gcgagggcac
     2161 cggccgcagc ctcgacctcg acccgtggga cagctggtac gaacacatcg tgctgtggga
     2221 cgccggcgcc gcaaggatcg ccggtgccta ccggatcgcc cgcggtgcgc cggtactggc
     2281 cgcgcacggc atccgtggcc tgtacaccgc ctcgctgttc gactacgcgg acgacgccat
     2341 cgcccgcatc gcgcagggga tggaactcgg ccgcagcttc gttgccccgg actactgggg
     2401 cagccgcagc atcgactacc tgtggcaggg catcggcgcc tacctgcgcg cgcatccgaa
     2461 ggtgcgctac ctgttcggcg cggtttcgat cagcgcggcg ctgccgcccg ccgcgcgcga
     2521 gcagatcgcc gcgcactacg cgcattactt cggcggcgat gccggcgagg tggtgtcgaa
     2581 gcggccgttc gcctaccgcg ccgcgcagcc gcgggacgcg gcggtggatg cagagaccgc
     2641 gttccgggtg ctgaaggcga acctcgacgc gctcggcgcg caggtgccga tgctctacaa
     2701 gcagtacacc gaattgtgcg agccgggcgg cgcgcgcttc ctcgcgttcg gggtggaccc
     2761 ggacttcagc gatgcggtcg acgggctgat cgaagtcgac atccagcgca tgcggccgaa
     2821 gaagcggcaa cgctacctcg gcgaagcgaa gccgggcacg accccgcacg ccgcagcgga
     2881 ggcggcatga atcccgccgc cgcgccggtc ccgctgcggc gcgcggtgtt catctccgac
     2941 gtccacctcg gctcgcggca ttgccatgcg gcggaactgg cggacttcct ggcgcgcctg
     3001 cgctgcaagc ggctttacct ggtcggcgac atcgtcgacc tgtggtggat ggcgcaacgc
     3061 cgcgccagct ggggcgcggc gcagaaccgg gtggtcgagg cgctgcacgc gctgcgtcgc
     3121 gccggcaccg agatcgtgta catccccggc aaccacgacc gcccggtgcg ccgcttctgc
     3181 ggcctcgccc tgccggcgat gcgggtgcgc cgccgcgcga tccacgtcac cgccgatggc
     3241 cgccgcctgc tggtgaccca cggcgacgaa ttcgacgcga tcacccactt cggcagcctg
     3301 caggagaaat tcggcgactg gctgtactac cgcatcctcg gtggcaacca gctgctcaac
     3361 ttcgtgcgcc gccggctggg gctgcgctac tggtcgcttt cggaattcct gaagcgccag
     3421 agcggcgccg ccgagcgctt catcgaccgc ttcgtccagg ccggcctcga cgacgtgcgc
     3481 cggcgcggcc tcgacggcat cgtctgcggc cacatccatc gcgccgccct ggtccagcgc
     3541 gatggcctgg tctacgcaaa cgacggcgac tgggtcgaaa gcctgagtgc gctggtcgag
     3601 gaggccgacg gcagccttcg cctgcttgcg cacaccggcg aagtgctggc cgaggtattg
     3661 ccgcgatggc gaccgatgcg atccccgggc gtcgggacgc tgccgcaagc ggcgtgatcg
     3721 ccgcgcccgg cgccggttag gatcggcgca tggattcgct gacccagatc gtcctcggcg
     3781 gcgcggttgc cgccgccatc gcgcccgcac cacatcgccg cgccgcgttg ctcgctggcg
     3841 cgatgctcgg caccttgccc gacctcgacg cgctgccact ggccctgctc accgacgacc
     3901 cggtcgcgcg catgacctgg catcgcagcg ccagccattc gctgttcgtg ctgccgttgc
     3961 tcgggtgggc gatctgggcg ttgttccgac gcagcggcgg gcgcgtggcg gaatcgccgc
     4021 tgcgctggtt ctgggccatc cagttggcgt tggtgaccca tccgctgctg gatgcattca
     4081 ccgtctacgg cacacagctg tggtggccgt tgccgatgcg gtcggtgatg tggtccagcg
     4141 tgttcatcat cgatccgctg tacacgatct ggttgctggc cgcgtgcatc gcggcctggt
     4201 ggctgcgcga gcggatcgcc gcgcaacggg tgctgctggc cggcctggtg ctgagcagcg
     4261 cgtacctcgc gtggtccttg ctggcgaagg tggcggtcga tcgcgaagcc ggccgcgcgc
     4321 ttgctgcgat cggactggcg gatgcgccgc ggttttcggt gccgatgccg ttcaacaccg
     4381 tgctgtggcg ggtggtggcg atgaccccgc agggtttcgt cgagggcgaa cgctcgctgc
     4441 tcgccgacag cgggccgatg gtgttccggg ggtatcgctc gaacctggag gcgctgcgtg
     4501 ccgcgcaggg gattgccgcc gtgcagcggc tggactggtt caaccacggc ttccagaagg
     4561 cgcaggtgcg cgacggcgtg ctggtgctca gcgacctgcg gatgggcgcg gaaccggatt
     4621 acaccttcaa cttcgcggtc gccgagcgcg acggcaacgc atggcgcgcg atcccgcccg
     4681 agcaactgcg ctggccatgg caggccacgc gccgcctgac cgcgatgtgg gagcggatct
     4741 ggacccagcc cgctttggct caggccgcgg caggcaaccc ggtcgccacc cctgcggcca
     4801 gcgcatcgaa gtaatcgcgg gtcagccgca gttgttcgtc ggcggtctgc gcgctgttca
     4861 caaccgaacg gaacgccgcg ttctcggggc ggtccttggc gtaccagccg aggtgcttgc
     4921 gcgcgatgcg cacgcccgtg gcctcgccgt agaacgcatg caggtgcccg aggtggccga
     4981 gcaggatgtc gcgcacctgc cgcagcgaag gctccggcac caattcgccg gtggccaggt
     5041 agtggccgat ctggccgagg atccacggac gaccctgcgc cgcgcgcccg accatcaccg
     5101 catcgcaacc ggtggcatcc agcacctgtt tcgccttccg gggggaatcg atgtcgccat
     5161 tggcgatcac cgggatccgc aggatcgact tgatccgggc gatggtgtcg tactcggcgc
     5221 tgccggcgta ctgctggtcg cgggtgcggc catgcacggt gagcgcggcg atgcccgcgt
     5281 cctcggcgat gcgcgcgatc tccgccgcgt tgcgatggct ggcgtcccag ccggtgcgga
     5341 tcttcagggt caccggcaca tcgaccgatt ccaccaccgc cgcgaggatt cgccccacca
     5401 gctgcggttc gcgcatcagc gccgaacctg cccacgcgtt gcacaccttc ttggccgggc
     5461 aacccatgtt gatgtcgatc agctgggcgc cgtgctcgac gttgtggcgc gcggccgcgg
     5521 ccatcaccgc gggcacggtg ccggcgatct gcacgctgac cggatccggc tcgccgacat
     5581 ggtccatccg gtgcaacgat ttcgcggtgc gccagaagcg cggatccgag ctggtcatct
     5641 ccgacaccgc catgcccgcg cccaaccgct tgcacagcag ccggaacggc ttgtcggtca
     5701 cccccgccat cggcgcgagg atcgtcctgg attcgatgtg gtggcgtccg atccgcatcc
     5761 gcgcattgta cggatgcgcg cgttcgcgcc gccctggctc aggcggtcgg cagccgcggc
     5821 atcgccagcg ccgcgccttc gcattcggtc gacaggccgg cgtagcgggt cgcgaagcgg
     5881 atgtgttcgg cgaacggttg ccgcggccgc aatgccagcg aagccgccag cgcgccgttg
     5941 aaggcgtcgc cggcgccggt ggtgtcgacg gcgttggcgg cgtcggcgga gatgcggtag
     6001 caggcggcct cgtcgccgcg caggttgccg tccgggtggg agacgaagca gccgctggcg
     6061 cccatcgtca ccaccaccga gctgccatgc ccgagctggc ggcacaacgc atgcaggcgg
     6121 ccctggtcga ggctggacac ggcgtcggcc tcgatccgtt cgccgacgtg gcggcccagc
     6181 agggcggcga attcggtctc gttcggggtc aggatgtcgg ccagttccag caacgcgccg
     6241 ctggccgttg cattcgccgg cgccgggttg aggatggtgg tcgcgccgcg cgcgcggcca
     6301 tgggtgagcg cgcgtgcgat cgcatccgcc ggcgactcca gttgcgcaag caccaccgaa
     6361 accgcgccaa gcgcctccag cgaggcggcg acgaacccgg catccagggt tgcattggcg
     6421 ccggcgccga ccacgatgca attgcggccg gacgcatcca cgaagatccc ggcggttccg
     6481 gtcggcgcag cactttcggc atcgcgcagg tcgatgccgt cggcggtgca cagcgcgcgc
     6541 gcgagctgcg caccgaggtc gccgccgagc gcgcacacga agcaggcttc ggcgccggcg
     6601 cgacgcgcgg ccatcgcctg gttgaaaccc ttgccgcccg ggccgctggc atagtcgccg
     6661 ccgagggtgg cgcccgcctg cggcagcgcc gccacccgcc acacgtggtc gacgttgaac
     6721 gagccgacga cggcgacgcg cgccatcggg tcagccgccg aaggcgctga gcacgcccgc
     6781 gatggtggcg gtcatgaagg tcgcgatcga gccgcccagc accgcgcgca ggccgaaccg
     6841 ggcgaggtcg ctgcggcgct cgggggcgag cccgccgatg ccgccgatct ggatcgcgat
     6901 cgagctgaag ttggcgaatc cgcacagcgc ataggtcgcg atcagcttgc cctgttcggt
     6961 cagggcgacc cccggggtgt tgccgttgac gatctcggac agctgcaggt aggcgacgaa
     7021 ctcgttgatc accaccttct gcccgatcag cgagccgacc gtgctggcgt cctgccacgg
     7081 caccccgatc acccacgcca gcggcgccag cacccagccg aagatcgcgg cgaggtcggt
     7141 cggcttgccg agcgtcgccg acaggccgct gacctcgccc agccacgtga gcggtgcatt
     7201 gatcatcgcg atcagggcga tgaaggcgag cagcatcgcg gcgatgttga gcgacagctt
     7261 gaggccctcg gcggcgccgc cggcggcggc gtcgatcacg ttgctggcgg tcttctcgac
     7321 ctccatcttc acggtgccgc gggtcagcgg ttcgccggtt tccgggatca gcagcttggc
     7381 caccaccagg gtggcgggcg cggccatgat cgaggcggcc agcaggtgct tggcgtagaa
     7441 cgcctgctgc accggatcgc cgccgccgag catgccgacg taggccgcga gcacgccgcc
     7501 ggcgatgtgc gccatgccgc cgatcatcat cgtgatcagc tcggactcgg tcatccgcgc
     7561 gatgtacggg cgcaccgtga gcggcgcctc ggtctggccg atgaacacgc tggcgcagac
     7621 gctggtggtc tcggcgccgg acacccgcat gaccttggtg atcgcccacg ccatgccgcg
     7681 gacgatccac tgcatcacgc ccaggtggta gagcacgccc atcagcgagg cgaagaagat
     7741 gatcgtcggc agcacctgga aggcgaagat gaagccgaac ttcgaggtat ccatcagcga
     7801 gccgaagatg aagttcgaac cggcggcgac gaagctcagg accttgacga agccgcggcc
     7861 gagcgcgtcg aacacgtcgc ggccgcctgg caccagcagc accagcgcgg cgaacaggat
     7921 ctgcagggac acgccggtgg cgaccagctt ccagtcgacc gcacgcctgt tgctggagaa
     7981 caaccatgcg atgccgagca gcaccgcaag gccgaacagg ccgaagccga tccgcccgag
     8041 agcttccatc cgaatccccg cctgcgttgt tgacggtgca gggtaggtca gcccccgcga
     8101 cccggcaagc gccgtcgctc aggggtcgcg cgccagcacc cggttgcggc cttccagctt
     8161 ggcgtggcgc aggcgcaggt cggcgcggcg cagcagctgc gagaggtcgg cgccgtcctg
     8221 cggccagctg gcgatgccgg cgctgaaggt cagccgcatc aactcggccc cgtgctccgg
     8281 cgcgaacggt tgcgtggcca ctgttcggcg catcgcttcc agccgttccc aggcgcgccc
     8341 cagcggcagc ggcagcagca gcgcgaattc ctcgccgccc agccgcgcca gccagtcgcc
     8401 ctcctgcatc tgctgccgca gcaccccgac cacgtggcgc agcgcgcgat cgccggcgag
     8461 gtggccgact tcatcgttga tccgcttgaa gtagtcgagg tcgatcaggc acaccgccag
     8521 cgacccgccc tggccgcgga tggcgtcgag catgaccggg aagcggtgca ccagccaggt
     8581 gcggttgggc agcccggtca ggccatcggt gccggacatg tccaccagtt tctgcatgcg
     8641 gtagacgatg gtcgcggtga tcgcggtcat cagcgccagc agcaccagcc gctggatctg
     8701 gttggacgcg gtcacggtgc cgtagtcgat cgaggccagc tggtccgggt tcggcaccag
     8761 ggcgaagatg gccgccgcca gcacgccgta ctgcgcaatc gccagcgcgc cactgaacag
     8821 ggtcaggcgg ccgtcgttgc gcaacgcggt catcgcgatc gagaccaggt agaacgccca
     8881 caccaccatg ctgttgagcc cggtgaccgg cgaatcgacg gcgaggatgg ccagcaccag
     8941 ggtggtcagg ctgacatcgt aggcggcggt cgcccacggc agccagcgat gccgctggcg
     9001 ttgccgggcc agcgccagcc acgcctgcga cagcacgatc gccagcacca cgccgaacag
     9061 gccgaccatt gtctcgctgg ccttgccgcc cgccagcagg ttgaccagcg gcatcgacag
     9121 catcgccatc gacagcaagg cgcgcagccg cgccaccagc aattcgcccc ctgcgccgag
     9181 ctccagcatc agcgcatccg gccgcgccag caggcgccgc cggaaatcgc gcaaacccgc
     9241 aactgcgtca gtctcgaagg cccccatgcg cggccagcat aacgcgccgg tgcgcgatca
     9301 gccgccgacg ccggatagcg ccgcccacgc gccgagcgcc aggaacacgc aggccgcggc
     9361 gatccgcgcc gccttcagcg gcagcctggc agcgaagcgg ctcccaagcg cgaccaccgg
     9421 cacgttggcc agcagcatgc cggcggtggt gccggcgacc acctgccaca gcgaggcgta
     9481 cttcgccgcc agcagcacgg tggcgacctg ggttttgtcg cccatctcgg cgatgaagaa
     9541 cgcgatcgtg gtggcgatga aggcaccccg cagcggcagg cgttcgtcgt cgtcgagctg
     9601 gtcgggcttg agcgtccaca gggcgatgcc gatgaaactc gcggcaacga cccagcgcag
     9661 catctgcggg gtcagccagc ctgccgcgag ttggcccatc catgccgcca gcgcgtggtt
     9721 gagcagggtc gcgaccagga tcccggcgat gatcggccac ggcttgcgga agcgcgcggc
     9781 cagcagcagc gccagcagct gggtcttgtc gccgatctcg gccagggcca cggtgccggt
     9841 ggcgacaagc agtgcatcga gcatcggcgg cggcatccac ggcgtgggaa aggcgcgatt
     9901 ctagcggtgc ccgcgcgcgg cgatggctac actgcgcacg aaaccgcacc ggagccacgc
     9961 atgcaatacc gccgcctcgg cacctcgggc ctgcaattgt cggcgctggc gttcggcgcg
    10021 tgggtcacct tcggcaacca ggtcggccgc gggaccgcgc gcgagctggt cgccgcggcc
    10081 tgggaccacg gcgtcaactt cttcgacaac gccgagggct atgccaacgg cgaggcggag
    10141 aaggtgatgg gcgacgtgat cgccgacctg cgcctgccgc gcgatggctg gtgcgtgtcg
    10201 agcaaggtct atttcggttc ggcggcggat ccgcggccga cccagaaggg gctgtcgcgc
    10261 aagcatgtgc atgatgcctg ccatgcggcg ctgaagcggc tgcgggtgga ttacctcgac
    10321 ctgtacttct gccatcgccc ggacccgcaa acgccgatcg ccgaaaccgt ttcggcgatg
    10381 gacgccctca tccgccaggg caaggtgctg tactggggca cctccgaatg gagcgcgggc
    10441 gagatccgcg aggcccaccg cgtggcgcat gcccacaacc tggtggcgcc gaccatggaa
    10501 cagccgcaat acaacctgct cgagcgcgaa cgcgtcgaag tggagtacgc gccgctgtac
    10561 gccgaattcg gaatgggcac caccatcttc tcgccgctgg catccggcct gctgaccggc
    10621 aagtacaacg acggcatccc ggccgggtcg cgcctcgccc aggacggcat gggttggctg
    10681 cagcgcggcc tgcacgggga ccgcatcgag gcactgcggc gcttcacttc gctggcggcc
    10741 gaacttggcg tggcaccggc gaccctggcg atcgcctggt gcctgcgcaa tccgaacgtg
    10801 tccagcgtgc tgctgggcgc aaccggactg gcgcagctgc tgcaggacct cgaggcgctg
    10861 gaagtgtggc agcgcctcga cgacgccagc tgggagcggg tgcaggcaag cctggcctga
    10921 gcacgcgccg gcgccgatat cgcccgccat gcccggcgcc tatactcggg cgaacaaatc
    10981 cggaggtggt tccatgggcc cgctactcgc gttgatcgtg ttgttcgccg gtgtcgtcat
    11041 cctgttcaag accgtgcgga tggtgccgca gggctacgaa tggacggtgg agcgcttcgg
    11101 caagtacacc cacaccatga ccccgggcct gcacttcctg gtgccggtgg tgtacggcgt
    11161 cggccgcaag atcaacatga tggagcaggt gatggacgtc cccagccagg acgtgatcac
    11221 caaggacaac gcggtggtca aggtcgacgg cgtggtctat ttccaggtgc tcgacgccgc
    11281 caaggcggcg tacgaggtcg ccaccctgga gatcgccatc ctcaacctgg tgatgaccaa
    11341 catccgcacc tcgatcggct cgatggacct ggacgaatcg ctttcgaagc gcgacgagat
    11401 caacgccaag gtgctggtgg cggtcgacca ggccacccac ccgtggggcc tgaaggtcaa
    11461 ccgcatcgaa ctgaaggaca tccagccgcc gcgcgacctg gtcgactcga tggcacggca
    11521 gatgaaggcg gagcgcgaga agcgcgccaa catcctggag gccgagggct tccgccaggc
    11581 ggcgatcctc aaggccgagg gcgagaagca gtcggtgatc ctggaggccg aaggcgagaa
    11641 ggaagccgcg ttccgccagg ccgaggcgcg cgagcgcctg gccgaggccg aggccaaggc
    11701 gacccagatg gtctccgacg cgatcgcctc cggcaacgtg caggcgatca actacttcgt
    11761 cgcgcagaaa tacatcgagg cgttcaaggc gctggccgaa ggcccgaacc agaagttcgt
    11821 gctgatgccg atggagtcgg ccggcgtgat cggttcgctg ggcggcatcg ccgaactggc
    11881 gaaggaggcg atggaccggc aggccggcgg cgccaaggtc atcccgtcgc cgaaggccgg
    11941 gggctgagcc atgcgctggg acgtgttcgc gtgggcggcg atcgcgctgc tgctgttcgc
    12001 cgcggaagcg ctggcccccg gtgccttcat gctgtggctg ggcttcgccg cggtcgcggt
    12061 gttcctcggc gtgctgctgg tcccgggcat cccggtgctg gcgcaggccg cggccttcat
    12121 cgcgctcggc ttcgtctcga tccaggtcta ccgcaagtgg ttccgcaccc gcgagcgccc
    12181 gagcgaccag cccgcgctca accgccgcag tgccgcgctg gtggggcggg tggtgccgct
    12241 ggaacgcgcg atcgtcgatg gtcgcgggcg ggtgcagatc gccgacgcct tctgggaagt
    12301 gaccggcccg gagctgcctg ccggtaccca cgtccgcatc gtcggcggcg acgcgatgag
    12361 cctgcaggtc gagccggcct gatccggcgc gcgccgccac gtaccggcgc gcatggggtg
    12421 cccgctctgg gataatggcg gccccacgtc gccggacccg ccgcatgacc cagaagacca
    12481 tcctcaacga gacccaccgc gcgctgggcg cgaagatggt cgatttcggc ggctgggaca
    12541 tgccgatcca ctacggttcg cagctcgacg aacaccacct ggtgcggcgc gacgccggca
    12601 tgttcgacgt cagccacatg accgtggtcg acctcaccgg tgcgaaggtg cgcgacttcc
    12661 tgcgccacct ggtcgccaat tcggtggaca agctgcagaa gccgggcaag gcgctgtaca
    12721 ccgcgatgct caacccgcag ggcggggtga tcgacgacct gatcgtctat ttcatgtcgg
    12781 aagactggtt ccggctggtg gtcaatgccg cgacccgcga gaaggacgtc gcctggatca
    12841 ccgcgcaggc caccgcgttc gcggtcgacg tccgcgagcg tcccgaattc ggcatggtcg
    12901 cggtacaggg cccgaacgcc cgcgaaaaag tcgtcggcct gctgcgcgag gaagaccgcg
    12961 cgccggtgtc gaagctgctg cgcttcagcg cgcgcgaggc caggaccacc gatggcgtcg
    13021 aggtgttcgt cgcccgcacc ggctacaccg gcgaggatgg cttcgaggtg atcgtgccgg
    13081 aggcgcagac cgtggcgttc tggaatgcgt tgctggaggc cggcgtgaag ccggctggcc
    13141 tgggcgcgcg cgacaccctg cgcctggagg caggcatgaa cctgtatggc caggacatgg
    13201 acgacaccgt ctcgccgttc gaggcagcgc tcgcctggac cgtggtcctg gacgagggcc
    13261 gcgacttcat cggccgcgcc gcgctggaag cgcagcaggc ggctggcgtg tcgcggcaga
    13321 tgatcggcct ggtgatggac gagaagggcg tgctgcgaca cggccagaag gtgctgaccg
    13381 ccaacggcga gggcgagatc ctgtccggca ccttctcgcc gtcgatcggc aaggcgatcg
    13441 ccttcgcccg cgtgcccgcc ggcgcgccgg gcgaggtgcg cgtcgacatc cgcggcaagg
    13501 aagtcccggt gcgcgtggtg aagttcccgt tcgtccggga aggcaaggtc caggacggca
    13561 tctgacccga cgcgggtgaa cattcgcccg cagcttcgct agactttccc caaccaattc
    13621 cccgcaagtt ccactggagc cagcaatgag cgagatcccc ggcgacctca agttcctcaa
    13681 gtcccacgaa tgggcccgtg tcgaaggcga cggcaaggtc accgtcggca tctccgacca
    13741 cgcgcagggc ctgctcggcg acctcgtcta cgtcgagttg ccgaacgtcg gcgacagcgt
    13801 cgaggccggc aatgcctgcg cagtggtgga gtcggtgaag gccgcgtccg acgtgtacgc
    13861 gccggtcagc ggcaaggtgg tcgaggtcaa tgccgcgctg tccgacaagc cggagaccat
    13921 caacgaggac gcctacggcg acggctggct gttcaccatg gtcatcgacg acgccgagca
    13981 gctcaacgag ttgctcgacc cggacgccta cgcggagctg ctggaagaag aagaccactg
    14041 acgcgcgcgt ccaaccgaac accatgccgg gacccgcggt ggaaacgccg cgggtccttc
    14101 gttttccggg gcagggaaaa gcgcgatggc ggcgcgtcga tcgctttcga tgcgcgaaaa
    14161 tgttcgcgca gttttgcgca aacgggcttg tgcaacgcgc aaaggcatcg cttcgcgcgc
    14221 gatcgattcg tccgcaagga aagcgaagga acggcatcgc ggagatgcgc ggagaatgcc
    14281 gcgtacttcg cgcgcgcatg catcgatgca tgcgcatgat cgctgcaagc cgcgtcattg
    14341 cttgcgcgtg gcattcgcgc aaggcgatgc gcgcggttgc gcgtgggtga ggtgcggccg
    14401 ccttcgttgc tgcgcgagcg catcgttgac caacgccatg cgcgtgtcgc gaaaaataat
    14461 tgcgacagga tgttgacagt tgaaaaaacc gtgattaggt ttcgcccagc agacgtttcc
    14521 tgcagaagcg agtgataaaa caaatcgcga taaccggccg cagcaaacgc acctccgccc
    14581 gcaacattga aaaggtggac gccggcatcg catccctcgc agaaagcttg gaagcgccca
    14641 tgatcgaagc ctgcagcgtt cgtcgccgcg ggttttttaa tgggtgcgga acgtcagacg
    14701 gcgctggcgc ggttgcatcg cgcggcgcca cccaatccgt accggtgcgc ctggcgcgcc
    14761 ggcggtcgat cgcgggtccg atacccgtgg tgttttctgc aactgggcgt taccgctgta
    14821 cccattgacg aggagccata ccatggccgt taagaaagct gctgcaaaga aggctgcgaa
    14881 gaagccggcc gccaagaagg cggcgaagaa ggtcgccaag aagccggcgg cgaagaaggt
    14941 tgcgaagaag gctgctgcga agaagccggc cgccaagaag gcggtgaaga aggctgctgc
    15001 gaagaagccg gccgccaaga aggcggtgaa gaaggctgct g
//
LOCUS       DAORUK010000043        14907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_3813_length_14907_cov_19.086520, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000043 DAORUK010000000
VERSION     DAORUK010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 14907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14907
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3813_length_14907_cov_19.086520"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PK818_04465"
     CDS             <1..672
                     /locus_tag="PK818_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014504592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQY82023.1"
                     /translation="LSLLFLLGYVVPQFSAMYDSLDADLPWFSQLVLGLGNFVRDWWI
                     VLLAIPLLALLWLDRKRRDPAFMLAFDGWLLRNALAGPLLAKLEAARLARTLGTLLRN
                     GVPLLSALGIARNVLDNRVLANDVDAATDEVKNGIGLSAALARGKRFPRLALQMIQVG
                     EESGALDAMLLKTADTFEQETAIALDRMLAALVPAVTLVLATVVGIVILAVLSPIYDL
                     TSVVG"
     gene            764..1195
                     /gene="gspG"
                     /locus_tag="PK818_04470"
     CDS             764..1195
                     /gene="gspG"
                     /locus_tag="PK818_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003490502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HQY82024.1"
                     /translation="MRNRRIQPSFAGSSQAGFSLIEIILVVVLIGGIVAFAATRILGG
                     GDRAKVNLARAQVQTLAEKVEQFEMDTGALPGALQDLVKAPGDAAGWLGPYAKESELK
                     DPWNHPFEYAMPGEGRPFDLLSRGKDGEPGGDSVDADIKYE"
     gene            1225..1698
                     /locus_tag="PK818_04475"
     CDS             1225..1698
                     /locus_tag="PK818_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012479113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HQY82025.1"
                     /translation="MHARRPASGFSLLEMLLVMVLVAAASLLAVAAFGGGMRGMALRG
                     GANDLAAQFRFARAVAISSGEPQDVVIDPQARQWKGAKGRSGALPDGGEVVFTGARAA
                     QFDPAETEGGKGVVRFFADGAATGGRVRMLANGAGWDVDVGWLTGEVRVSRVRAP"
     gene            1695..2123
                     /locus_tag="PK818_04480"
     CDS             1695..2123
                     /locus_tag="PK818_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HQY82026.1"
                     /translation="MMNTRAQAGYTLIEIVVAFAILALGLTLLLGTLSGATRQVRQAG
                     DAGRAALHAQAVLAEFGELPQPGVREGELEQGRYRWHLEVAPWSDPEPRRGPRRSDPN
                     AARLLHLRLQVDWGEGGPRQRVQLSSLRLVLPPVEGVAAP"
     gene            2120..2770
                     /locus_tag="PK818_04485"
     CDS             2120..2770
                     /locus_tag="PK818_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQY82027.1"
                     /translation="MSQARRRASGFTLIEVLLATMLLAAGLVLAFATVRAAAATVQRG
                     EAMAARNERMRAVSEFLRRRIGGAQGIAFGLDDDSGASLRFTGEARRMRFVADLPDYL
                     GRGGPHLHELGVVRDGSGQSLQVDFRMVLAGEPIADTRARPPEPLAGGLRGVEFAYRT
                     LGKRGVPGPWLYQWEHPDALPMQVRVRIADAQGAWPDLVVSLPLAASYGVAPEDAP"
     gene            2806..3591
                     /locus_tag="PK818_04490"
     CDS             2806..3591
                     /locus_tag="PK818_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HQY82028.1"
                     /translation="MWLVLLLSGLVAGYAMAARIESMQGNGLARAAAAREAARAGVEY
                     AALRLLDPDRTARWAADGRGYRLAFDTAQLDITVRDEAGKVDLNAAGHPLLLALFVAL
                     GEPRDAAGRLAGAIVDWRDEDGLTQPAGGAEDPDYAAAGLAWGAKDAPFETVAEVEQV
                     LGMRPALFASAAPYLTVYSGSAMPDAASADGIVLQAMGVDRPQPPGPDAMPLAGSGTY
                     SIDSRARLADGRRAHVSVILRLGGNALPGSAYTPLRWQDGASP"
     gene            3588..4730
                     /locus_tag="PK818_04495"
     CDS             3588..4730
                     /locus_tag="PK818_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017914613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HQY82029.1"
                     /translation="MSAVLSPLARFESAGRSLRGLMAWWGAGLAAWLPAGWRQALAAS
                     TDRLLLQVQGEGLQLRRQAGGGLQDVASLPVPAAAGEGIDPLAGVLATQAADMPRWLL
                     LPAASGLRRNLLLPAAARERLREVLGFEIERQTPFAAADVLYDGRVLGVRDDGQLQAE
                     LVVVPRARADAATARLGGLAGWLAGIDLADAEGRPLGVNLLPPAQRYRRANPWRGWNL
                     VFAAVALIALLLGLSQVVENRRAAVTRLQAEVAQRSAQARNVAQQRQRLVDAVEGGTY
                     LQAQRNARPSVIEAMDELARRLPDGTYLEKVSIEGGQLTLIGLSNQAAALVGKLEGAR
                     QWRAPALSGALQPDVRTRSDRFTLVAQLNDAAAPPPASGAVSRAPR"
     gene            4717..5349
                     /gene="gspM"
                     /locus_tag="PK818_04500"
     CDS             4717..5349
                     /gene="gspM"
                     /locus_tag="PK818_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HQY82030.1"
                     /translation="MPRADRDRWLALALLLAVLALSYLLVVHPWFTRPLQEADARIAE
                     LQARDARARALLQQAPQIRQRLGELDARGSATGFLAEATTELATAALIQQLERVVAEV
                     SPGNRGCAITNRAPLTGEPPPGRYRRVTVQVRLRCGNAETLAVLHALESARPYLFVDV
                     VNIAAQRYFAIPGNNLPQEGGLDVSFDLYGYLRPALVPLPPATGEGASRG"
     gene            5342..6088
                     /locus_tag="PK818_04505"
     CDS             5342..6088
                     /locus_tag="PK818_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82031.1"
                     /translation="MAERGLLQRFDQAGPLTWLLAAGAGWALLLWFGALLGMGGQVSQ
                     AQASAQAQLPQPGKATPDRIGPLAQYADAASRPLFTEDRRPRSFLATAGEGDGAAQSQ
                     ALDFILTGVLISPQVQLAVLQPSGGGEAQRVRVGKSPEGAAGWRLLEVQPRRAIFEGG
                     GGQTALDLRTFGDAGAPTATAMASPPPAPAMATAKPPPPRQDAGAAEPQNQEARIDAI
                     RRRIEARRAQMRAGQNGGSGPAASTQPPKP"
     gene            6105..8258
                     /gene="gspD"
                     /locus_tag="PK818_04510"
     CDS             6105..8258
                     /gene="gspD"
                     /locus_tag="PK818_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HQY82032.1"
                     /translation="MNVRPFVATALLGLLAACASAPSPRMQRADQLPGEQVGGDASVR
                     TDALPEVMGNAGPRPVIRRGSGAPINQSAASAPPPSLASSGQASFNFEGESLHAVVKA
                     ILGDMLGQSYSIAPGVQGTVTIATQKPVGSAGALNLLEGVLAQNNARMVFSDGRYNIV
                     PADQALASGVVPRTGSPAMARGYESRVVPLRYVSAAEMEKILKPYARQGAIVSVDGAR
                     NIITVAGTRAELENYLRTVQVFDVDWMASMSVGVFPLQSGRASQVVQDLERVFGEQSK
                     TPVAGMFRFMPLDAANAVMVITSQPNYLDDIQQWIDRIEGGSGEGRLFSYELKYIKAR
                     DLADRLAEVFGNGAGQSQQGEQPSLMPGVESTQIRSGGVDDKGGTSSAAIGSGGDGAS
                     LPSGGTGNGRVTLRVDGTEVGVSAVEDTNSLLVRASPAAWKSIREVIERLDVMPMQVH
                     IEAQVVSVVLSGALQYGVSWFFDNAIPEQTDFPYPTGRNSWGSYGSSVAPAEGFGNVL
                     TWSFLGNSAAAIVQALDNVSDVRTLSSPSVFTQNNKEASLNVGQRIPINSVSFDPTNS
                     GNSNGTYSQVQYLDTGIILKVRPRITRDGMVFMDIVQEVSKPVGAADVNGNVRIDTNE
                     VRTSAAIPSGETIMLAGLITDGSSKTSSGIPGLSRIPVIGGLFGQQGTSTTRDELVVL
                     ITPTVVRNPQEARDLTDEYGRRFRALDPIHDPKAK"
     gene            8255..9109
                     /locus_tag="PK818_04515"
     CDS             8255..9109
                     /locus_tag="PK818_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016901897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY82033.1"
                     /translation="MSPAASSLPIILVPVGVDEDALDACLAALDAGTPAGSRVWLADD
                     AQAGPRATAIIEAWLKHTPLQGAHTRRAASIGEAAHLDEALRACGNADVAILASDARP
                     APGWLQELAACLARDPTIATATPWSNAGEAASWPRVGEIAGLEEAPSRLARACVQLPA
                     RHPDLPAAVGHAVLVRGSARVRAGGLDPSSYRSWYAALIDLSLRLSGLGWRNALCETA
                     FVLRDGEGAPADGDMDRLSVRWPAWHARLANFLMGDPLHAVRAQLGARLAELDRGSPQ
                     QDLFAPAP"
     gene            9106..9939
                     /locus_tag="PK818_04520"
     CDS             9106..9939
                     /locus_tag="PK818_04520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY82034.1"
                     /translation="MNAAGIAAIVVSHGSAETLEDCLVRLRAAEGVAEIRVVDNDSRD
                     GSLEIVQRHALADPRVRFIANPDNPGFAVACNQGALDTRAPWLAFVNPDCFVQADSLA
                     RLRMHAAAIEPGLVGADLVGEDGARDPAARRRDPDFAAMLRDRARARLGVELQPSLPL
                     QVVDAVSGALMLLPRALFARIGGFDEGYRLHAEDLDLCRRARASGATVACANDVQVVH
                     VRGVSSRARPLFVEWHKHRGFWRYFNTFEAPRRGVATRGAVFAMIWGRFPLAAARAAL
                     R"
     gene            10110..11852
                     /locus_tag="PK818_04525"
     CDS             10110..11852
                     /locus_tag="PK818_04525"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HQY82035.1"
                     /translation="MFSNRTFVARESAPAAPVRGGILAGILGFALAACGGGGGRGAAP
                     ITPPPTTPPPAATISEAEAARFLIQASFGPTRAEIARVRELGYSRWLDEQMDPARTPP
                     TLVLPHIQQILANGLAETDLTQAHRRNAWLWFAANNRDQLRMRMAFALSEIFVVSDFE
                     SGRAQIPRVADYQDTLARNAFGSYRSVLKAVTLHPAMGVYLSHAGNRKASADGKIVPD
                     ENYGREVMQLFSIGLVERNQDFSPVLDGSGQTVPTYDSAVVAAMSRVFTGWTYAGQTD
                     ANYGRVDGRSYAPMECHPAYHDDQPKAIFRGIVIANGNDCAATLERALDALSQHPNTA
                     PFISRQLIQRFVTSNPSPGYIQRVGQAWSTSGGDLGRVLRAILLDGEARTAPAAGDAT
                     FGKPREPLLRIAHLWRAYEARYVPPASGALRFRAGSTADLALSIAQDSQRAPSVFNFF
                     EPDYRIPASDGSQGVYAPELQIVNESTFTTGHNQSDALLWNYASTSAPAANTNAPVLD
                     ITRLVQLAEAGDDAAMVQDVNLLLHGGSLPAASAATLTRMLGSLRAQGRGASERARSL
                     LLVALLSPDYAVQR"
     gene            11899..13347
                     /locus_tag="PK818_04530"
     CDS             11899..13347
                     /locus_tag="PK818_04530"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HQY82036.1"
                     /translation="MPHSTRHDRRRFLQGLGALVATGTAGALFPQLELMGHALAATPQ
                     AGDYRALVCIFLYGGNDSYNMLIPHAQAEYDVYLRSRGGVHDATSNPFGLGIARDSLA
                     TIADTSGGTWGLHPAFAAAKPLFDAGELAFLANVGSLVEPVRKSEVGYPGGKPLPPYL
                     YSHNDQQRQWMRGHATTTHAANGWGGLCGDRLAALNTGLSALPPTISIFGNNLYQSGA
                     SVLPYAVASNGPGELARMASNGGSADAIRLQALEELLHANQGHAMRTRYAGLGETSIM
                     VNGALRQALDPANGGDIATTFPGEPLAAQLRMIARLVKVSQGPAIGHKRQIFYAGLGG
                     FDTHDNQMDPTRHAALLSQVAGALAAFRAALQEIGMLDRVTTFTMSDFGRTLNSNGNG
                     TDHAWGGVQLVMGAAAGNGGSLQGRRVWGDYPLLELDGEQSMGRGRMIPTTSIQQVGA
                     TLASWMGVAEADLGTIFPGIGNFDAPRLGFLG"
     gene            complement(13354..14169)
                     /gene="pyrF"
                     /locus_tag="PK818_04535"
     CDS             complement(13354..14169)
                     /gene="pyrF"
                     /locus_tag="PK818_04535"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HQY82037.1"
                     /translation="MTFIEKLRNRWHSANSLVCVGLDPDPARFPEAFIDDEDALFSFC
                     RDIVDATAGFACAFKPQIAYFAAHNDGEAQLQRLIAHINGCFPDIPVILDAKRGDIGS
                     TAAQYAVEAFERYGADAVTLNPYMGRDSADPFLQYNDRGCVFLCHTSNPGACDFQELD
                     VGGEPLYQRIARTIAGEWNADRNCALVVGATFPEELKVIRGIVGDMPLLIPGIGAQGG
                     DVEATVRNGKTADGTGLVINSSRGILYASSGAGYADAAADAARSLRDEINRYR"
     gene            complement(14255..>14907)
                     /locus_tag="PK818_04540"
     CDS             complement(14255..>14907)
                     /locus_tag="PK818_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011346396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY82038.1"
                     /translation="IDDLSFSVPNGAIVGIIGPNGAGKSTLFRMITGQEKPDTGSIEM
                     GPTVKLAYVDQSRDKLEGNHNVFQEVSGGLDILNINGVEIQSRAYIGRFNFKGQDQQK
                     LVGTLSGGERGRLHMAKTLLQGGNVLLLDEPSNDLDIETLRALEDALLEFPGNTFVIS
                     HDRWFLDRIATHILAFEGDSHVEFFQGNYREYEEDKKRRLGEAGAQPHRLRFKALK"
BASE COUNT         2099 a         5210 c         5434 g         2164 t
ORIGIN      
        1 ctgtcgctgc tgttcctgct cggctacgtg gtgccgcagt tctcggcgat gtacgacagc
       61 ctggacgccg acctgccatg gttctcgcaa ctggtgcttg ggcttggcaa cttcgtgcgc
      121 gactggtgga tcgtgttgct ggcgatcccg ctgctggcgc tgctgtggct ggaccgcaag
      181 cgccgcgatc cggccttcat gctggcgttc gacggctggc tgctgcgcaa cgcgctggcc
      241 gggccgctgc tggccaagct tgaagccgcg cggctggcgc gcacgctggg cacgctgctg
      301 cgcaacgggg tgccgctgct gtcggcgctc ggcatcgccc gcaacgtgct cgacaaccgg
      361 gtgctggcca atgacgtgga cgccgccacc gacgaggtca agaacggcat cggcctgtcc
      421 gcggcgctgg ccaggggcaa gcgcttcccg cggctggccc tgcagatgat ccaggtgggc
      481 gaggaatccg gcgcgctcga cgcgatgctg ctgaagaccg cggacacttt cgagcaggag
      541 acggcgatcg cgctggaccg catgctggcc gcgctggtgc cggcggtgac cctggtgctg
      601 gccacggtgg tcgggatcgt gatcctggcc gtgctgtcgc cgatctacga cctgaccagc
      661 gtcgtcgggt gaagcaatgc gcctggccga tgccggcagc atcgcccccg gatctcggcg
      721 acaatcggcc tctccaacgt ccacccacca gcggaacccg ttcatgcgca atcgccgtat
      781 ccaaccatcg ttcgccgggt cgtcgcaggc cggcttctcg ctgatcgaga tcatcctggt
      841 ggtggtgctg atcggcggca tcgtcgcctt cgcggcgacc cgcatcctcg gcggcggcga
      901 ccgcgccaag gtcaacctgg ccagggcgca ggtgcagacg cttgcggaga aggtggagca
      961 gttcgagatg gataccggtg ccctgccggg tgcgctgcag gacctggtca aggcgcccgg
     1021 cgatgccgcg ggctggctgg gcccgtatgc gaaggaatcc gaactgaagg atccgtggaa
     1081 ccatccgttc gaatatgcga tgccgggcga aggcaggccg ttcgacctgc tcagccgcgg
     1141 caaggatggc gagcccggcg gcgacagcgt cgacgccgac atcaagtacg aataagcgat
     1201 gcgcgtggcg gcgggaatcg agtgatgcat gcgcgtcggc ccgcgtcggg attctcgctg
     1261 ctggagatgc tgctggtgat ggtgctggtc gcggctgcca gcctgctggc ggtggccgcg
     1321 ttcggcggcg gcatgcgcgg catggccttg cgtggcggcg ccaacgacct ggccgcgcag
     1381 ttccgcttcg cgcgggcggt ggcgatcagc agcggcgagc cgcaggacgt ggtcatcgat
     1441 ccgcaggcgc ggcagtggaa gggcgcgaaa ggccgcagcg gcgcgttgcc ggacggcggc
     1501 gaggtcgtgt tcaccggcgc ccgggccgcc cagttcgacc ccgccgagac ggaaggcggc
     1561 aagggcgtgg tgcgcttctt cgccgacggc gccgccaccg gtggccgcgt gcgcatgctg
     1621 gccaatggcg ccggctggga tgtcgatgtc ggctggctga ccggcgaggt gcgggtctcg
     1681 cgggtgcggg caccatgatg aacacgcgcg cgcaggccgg ctacaccctg atcgagatcg
     1741 tggtcgcgtt cgcgatcctc gcgcttggcc tgaccctgct gcttggcacc ctgtccggcg
     1801 caacccgcca ggtgcgccag gccggcgatg ccgggcgtgc ggcgctgcac gcgcaggcgg
     1861 tactggccga attcggcgag ctgccgcaac cgggggtgcg cgaaggcgaa ctcgagcagg
     1921 gccgctaccg ctggcacctc gaggttgcgc cgtggagcga cccggagccg cggcgcgggc
     1981 cgcggcgcag cgacccgaac gcggcgcggc tgctgcacct gcggctgcag gtcgactggg
     2041 gcgaaggcgg cccgcgccag cgcgtgcagc tgtcgtcgct gcggctggtg ttgccgccgg
     2101 tggaaggggt tgccgcgcca tgagccaggc acgccggcgc gcatccggct tcacactgat
     2161 cgaagtgctg ctggcgacca tgctgctcgc cgccgggctg gtgctggcgt tcgcgacggt
     2221 gcgcgcggcg gcggccaccg tgcagcgcgg cgaggcgatg gcggcgcgca acgaacgcat
     2281 gcgcgcggtc tccgaattcc tgcgccggcg catcggcggc gcgcagggaa tcgcgttcgg
     2341 cctcgacgac gacagcgggg cctcgctgcg cttcaccggg gaagcgcggc gcatgcgctt
     2401 cgtcgccgac ctgccggatt acctcggccg cggcgggccg cacctgcacg aactgggcgt
     2461 ggtgcgcgat ggcagcggcc agtccctgca ggtcgacttc cggatggtgc tggccggcga
     2521 gccgatcgcc gacacccgtg cgcgcccgcc ggagccgctg gccggcggct tgcgcggggt
     2581 ggagttcgcc tatcgcacgc tgggcaagcg cggggtgccg ggaccgtggc tgtaccagtg
     2641 ggaacacccg gacgcgttgc cgatgcaggt gcgggtgcgg atcgcggatg cgcagggcgc
     2701 gtggccggac ctggtggtgt cgctgccgtt ggcggcgagc tacggggttg ccccggagga
     2761 cgccccgtga gccggcgcca acggggtgcg gcgctgctgc tggtgatgtg gctggtgctg
     2821 ctgctgagcg ggttggtggc cggctatgcg atggcggcgc ggatcgaatc gatgcagggc
     2881 aatggcctgg cccgcgccgc ggccgcgcgc gaagccgcgc gggccggggt ggaatacgcc
     2941 gccctgcgcc tgctcgaccc cgaccgcacc gcgcgctggg cggcggacgg gcgcggctac
     3001 cggctcgcgt tcgataccgc gcaactcgac atcacggtgc gtgacgaagc cggcaaggtc
     3061 gacctcaacg ccgccggcca cccgctgttg ctggccctgt tcgtggccct gggcgagccg
     3121 cgcgatgccg ccggccggct ggcgggcgcg atcgtggact ggcgcgacga ggacggcctg
     3181 acccagccgg ccggtggcgc cgaggacccc gactatgcag cggccgggct ggcgtggggg
     3241 gccaaggacg cgcccttcga aaccgtggcg gaagtggagc aggtgctggg catgcggccg
     3301 gcgctgttcg cctcggcggc gccctacctg accgtctaca gcggcagcgc gatgccggat
     3361 gcggcgagcg ccgatggcat cgtgctgcag gcgatggggg tggatcggcc gcagccaccg
     3421 ggcccggatg cgatgccgct ggccggcagc ggcacgtata gtatcgacag ccgtgcccga
     3481 ctcgccgatg gtcgccgcgc acacgtttcg gtcattctgc ggttgggggg taacgctttg
     3541 ccgggttccg catacacgcc gctgcgctgg caggacgggg cttcgccgtg agtgcggtgc
     3601 tgtcgccact ggcacgcttc gagtccgccg gccgcagcct gcgcggcctg atggcgtggt
     3661 ggggcgcggg gctcgccgcg tggctgcccg cgggctggcg gcaggcgctg gcggcttcga
     3721 ccgatcgcct gctgttgcag gtgcagggcg aaggcctgca attgcgccgc caggccgggg
     3781 gcggcctgca ggatgtcgcc agcctgccgg tgccggcggc cgccggcgag ggcatcgatc
     3841 cgctggccgg cgtgctggcg acgcaggccg cggacatgcc gcgctggctg ctgctgcccg
     3901 cggccagtgg cctgcgccgc aacctgctgc tgccggccgc cgcgcgcgag cgcctgcgcg
     3961 aggtgctggg tttcgagatc gagcggcaga caccattcgc cgccgccgac gtgctctacg
     4021 acgggcgcgt gctgggcgtg cgcgacgacg gccagctgca ggccgaactg gtggtggtgc
     4081 cgcgcgcacg cgcggatgcg gccacggccc ggcttggcgg cttggcgggc tggctggccg
     4141 ggatcgacct ggccgatgcc gagggccggc cgctgggcgt caacctgttg ccgccggcgc
     4201 agcgctaccg gcgcgcgaat ccgtggcgcg gctggaacct ggtgttcgcc gcggtggcgc
     4261 tgatcgccct gttgcttggg ctgtcgcagg tcgtcgagaa ccgcagggcc gcggtgaccc
     4321 gcctgcaggc ggaggtggcc cagcgcagcg cgcaggcgcg caacgtcgcc cagcagcggc
     4381 aacggctggt ggacgcggtc gaaggcggta cctacctgca ggcgcagcgc aacgcgcggc
     4441 cgtcggtgat cgaggcgatg gacgaactcg cccgccggct gccggatggc acctatctgg
     4501 aaaaagtgtc gatcgagggc ggccagctga ccctgatcgg gctgtccaac caggccgcgg
     4561 cgctggtcgg caagctcgag ggcgcccgcc agtggcgcgc accggccctg agcggtgcgc
     4621 tgcaacccga cgtgcgcacc cgcagcgacc gcttcaccct ggtcgcccag ttgaacgatg
     4681 ccgcggcccc gccgcctgcc tccggagcgg tttcccgtgc cccgcgctga ccgcgaccgc
     4741 tggctggcgc tcgcactgct gctggcggtg ctggcgctgt cctacctgct ggtcgtgcat
     4801 ccgtggttca cccgcccgct gcaagaggcg gatgcgcgga tcgccgagtt gcaggcgcgc
     4861 gatgcgcgcg cgcgcgcgct gctgcagcag gcgccgcaga tccggcagcg gcttggcgaa
     4921 ctggatgcgc gcggcagtgc aaccggattc ctggcggaag cgaccaccga actcgccacc
     4981 gcggcgctga tccagcaact ggaacgggtg gtggccgagg tcagccccgg caaccgcggc
     5041 tgcgcgatca ccaatcgcgc gccgctcacc ggcgagccgc cgcccgggcg ctaccgccgg
     5101 gtcaccgtgc aggtgcgcct gcgctgcggc aacgcggaaa ccctggcggt cctgcatgcg
     5161 ctggaatcgg cgcgtcccta cctgttcgtc gacgtggtca acattgccgc gcaacgctac
     5221 ttcgcgatcc ccggcaacaa cctgccgcag gaaggcgggc tggacgtgag tttcgacctg
     5281 tacggctacc tgcgcccggc gctggtgccg ctgccgccgg cgaccggaga gggagcgtcc
     5341 cgtggctgag cgcggcctgc tgcaacgctt cgaccaggcc ggtccgctga cctggctgct
     5401 cgctgccggc gcgggctggg cgctgctgct gtggttcggc gcgctgctcg ggatgggggg
     5461 ccaggtcagc caggcgcagg catcggcgca ggcgcagttg ccgcagccgg gcaaggccac
     5521 gccggaccgg atcggcccgc tggcgcaata cgcggacgcg gcgtcgcggc cgctgttcac
     5581 cgaggaccgg cgcccgcgca gtttccttgc caccgccggc gaaggcgacg gcgcggcgca
     5641 gagccaggcg ctggatttca tcctgaccgg cgtgctgatc agcccgcagg tgcaactggc
     5701 ggtgctgcaa cccagtggcg gcggtgaagc ccagcgcgtg cgcgtcggca agtcgccgga
     5761 aggcgcggct ggctggcggc tgctcgaagt ccagccacgt cgcgcgatct tcgagggcgg
     5821 cggcggccag actgcgctcg acctgcgcac cttcggcgat gccggcgcac ccacggccac
     5881 cgcgatggcg agcccgccac cggcgccggc gatggccacg gccaagccgc cacccccgcg
     5941 gcaggacgca ggcgccgcgg aaccacagaa ccaggaagcg cggatcgatg cgatccgcag
     6001 acgcatcgaa gcccggcgcg cgcagatgcg cgccggccag aacggcggca gcggtcccgc
     6061 agccagcacc cagccgccga agccatgacg tggagttgtc gcgaatgaac gtccgtccct
     6121 ttgttgccac cgccttgctt ggcctgctgg ccgcatgcgc cagcgcaccg tcgccgcgga
     6181 tgcagcgcgc cgaccaactg cccggcgagc aggtgggcgg cgacgccagc gtccgcaccg
     6241 acgcgttgcc ggaagtgatg ggcaatgccg ggccgcgccc ggtgatccgt cgcggcagcg
     6301 gcgcgccgat caaccagtcg gcggccagcg cgccgccgcc gtcgctggcc agcagcggcc
     6361 aggccagctt caacttcgag ggcgagtcgc tgcacgcggt ggtcaaggcg atcctcggcg
     6421 acatgctcgg ccagagctac agcatcgcgc ccggcgtgca gggcacggtg acgatcgcca
     6481 cccagaagcc ggtcgggtcg gccggggcgc tcaacctgct ggaaggcgtg cttgcgcaga
     6541 acaacgcgcg catggtcttc agcgatggcc gctacaacat cgtgcccgcc gaccaggccc
     6601 tggccagcgg cgtggtcccg cgcaccggtt cgccggcgat ggcgcgcggc tacgagtcgc
     6661 gggtggtgcc gctgcgctac gtctcggccg ccgagatgga gaagatcctc aagccgtacg
     6721 ccaggcaggg cgcgatcgtc agcgtcgatg gcgcgcgcaa catcatcacc gtggccggca
     6781 cccgcgccga gctcgagaac tacctgcgca ccgtgcaggt gttcgacgtc gactggatgg
     6841 cgagcatgtc ggttggcgtg ttcccgctgc agtccgggcg cgccagccag gtggtgcagg
     6901 acctggaacg cgtgttcggc gagcagagca agaccccggt cgccggcatg ttccgcttca
     6961 tgccgctgga tgccgccaac gcggtgatgg tgatcaccag ccagccgaat tacctggacg
     7021 acatccagca gtggatcgac cgcatcgagg gcggcagtgg cgaaggccgg ctgttctcgt
     7081 acgagctgaa gtacatcaag gcgcgcgacc tcgccgaccg cctcgccgag gtgttcggca
     7141 atggcgccgg gcagtcgcag cagggcgagc agccgtcgct gatgccgggc gtggaatcca
     7201 cccagatccg cagcggcggg gtggacgaca agggcggcac ctccagtgcg gcgatcggtt
     7261 ccggcggcga cggtgccagc cttcccagcg gcggcaccgg caatggccgg gtgaccctgc
     7321 gggtggacgg caccgaagtc ggggtgtccg cggtggagga caccaactcg ctgctggtgc
     7381 gcgccagccc ggcggcgtgg aagtcgatcc gcgaggtgat cgagcgcctc gacgtgatgc
     7441 cgatgcaggt gcacatcgag gcgcaggtgg tcagcgtcgt gctctccggt gcgctgcagt
     7501 acggcgtcag ctggttcttc gacaacgcga tccccgagca gaccgacttc ccgtacccga
     7561 ccggccgcaa cagctggggc agctacggca gcagcgtggc cccggccgaa ggcttcggca
     7621 acgtgctgac ctggagcttc ctcggcaaca gcgcggcggc gatcgtgcag gcgctggaca
     7681 acgtgtccga cgtgcgcacc ctgtcctcgc cttcggtgtt cacccagaac aacaaggaag
     7741 ccagcctcaa cgtcggccag cgcatcccga tcaattcggt cagcttcgac ccgaccaaca
     7801 gcggcaacag caacggcacc tacagccagg tgcagtacct ggacaccggc atcatcctca
     7861 aggtgcgccc gcgcatcacc cgcgacggca tggtgttcat ggacatcgtg caggaagtca
     7921 gcaagccggt cggcgcggcc gacgtcaacg gcaacgtgcg catcgacacc aacgaggttc
     7981 gcaccagtgc ggcgattccc agcggagaga ccatcatgct ggccgggctg atcaccgatg
     8041 gcagcagcaa gacctcgtcc ggcatccccg ggctcagccg gatcccggtg atcggcggcc
     8101 tgttcggcca gcagggcacc agcaccacgc gcgatgaact ggtggtgctg atcaccccca
     8161 cggtggtccg caacccgcag gaagcgcgcg acctgaccga cgaatacggc cgccgcttcc
     8221 gcgcgctgga cccgatccac gaccccaagg cgaagtgagc ccggccgcgt cctcgttgcc
     8281 gatcatcctg gtgccggtcg gcgtcgacga ggacgcactc gatgcctgcc tcgccgcgct
     8341 ggatgccggc acccccgccg gcagccgggt atggctggcc gacgacgccc aggccggtcc
     8401 gcgcgccacc gccatcatcg aagcctggct gaagcacacg ccgctgcagg gcgcgcatac
     8461 ccggcgcgcc gccagcatcg gcgaggccgc gcacctcgat gaagccctgc gcgcctgcgg
     8521 caacgccgac gtggcgatcc tggccagcga tgcgcgaccc gcgccgggct ggctgcagga
     8581 gctggcggcc tgccttgcgc gcgatccgac catcgccacc gcgaccccgt ggagcaacgc
     8641 cggcgaagcc gcgagctggc cgcgggtggg cgagatcgcc gggctcgagg aggcgccttc
     8701 gcgcctcgcc cgcgcctgcg tgcagctgcc ggcgcggcat ccggacctgc cggccgcggt
     8761 tggccacgcg gtcctggtcc gcggcagcgc acgcgtgcgc gccggcgggc tggacccgtc
     8821 cagctaccgc tcctggtacg ccgcgctgat cgacctgtcg ctgcgcctgt ccggcctcgg
     8881 ttggcgcaat gcgctgtgcg agaccgcctt cgtgttgcgc gatggcgaag gcgcgcccgc
     8941 cgatggcgac atggaccggc tgtcggtgcg ctggccggcc tggcatgcgc ggctcgcgaa
     9001 cttcctgatg ggcgatccgc tgcacgcagt gcgggcgcaa ctgggcgcgc ggctggcgga
     9061 actggaccgc ggctcgccgc agcaggacct gttcgcaccc gcgccatgaa cgccgccggc
     9121 atcgccgcga tcgtggtcag ccatggcagc gccgagacgc tggaggactg cctggtgcgc
     9181 ctgcgcgcgg cggagggcgt ggccgagatc cgggtggtcg acaacgactc gcgcgacggc
     9241 agcctcgaga tcgtgcagcg ccatgcgctg gcggatccgc gcgtgcgctt catcgccaac
     9301 ccggacaacc ccgggttcgc ggtggcctgc aaccagggcg cgctggacac gcgggcaccg
     9361 tggctggcct tcgtcaatcc ggattgcttc gtgcaggcgg actcgctggc gcggctgcgc
     9421 atgcacgcgg ccgcgatcga accggggctg gtcggcgccg acctggtcgg cgaagacggc
     9481 gcgcgcgatc ccgccgcgcg ccgccgcgac ccggacttcg cggcgatgct gcgcgatcgc
     9541 gcgcgcgcgc ggctcggggt cgaactgcag ccgtcgctgc cgttgcaggt cgtggatgcg
     9601 gtttccggcg ctttgatgct gctgccacgg gcgctgttcg cccgcatcgg cggtttcgac
     9661 gaaggctatc gcctgcatgc cgaggacctg gacctgtgtc gccgcgcgcg cgcttccggg
     9721 gccaccgtgg cctgcgccaa cgacgtgcag gtggtgcacg tgcgcggggt gtcctcgcgt
     9781 gcgcgcccgc tgttcgtgga atggcacaag caccgcggct tctggcgcta cttcaacacg
     9841 ttcgaggcac cgcgccgggg cgtggccacg cgcggggcgg tgttcgcgat gatctggggc
     9901 aggttcccgc tggcggcggc acgggccgcg ttgcgctgat ccgggccgcg ggtcgcgccc
     9961 ccgccccgct ggtgccgctg cgccccggcc ccaatcggct gtcaaccgac cgcgtgcacc
    10021 cgcgttggca tcatcgagcg acgcgccggc ctgtcaccca tgcgggcgcc gcatgcgcta
    10081 cggtggcaac ggacccggag gggaaggcca tgttcagcaa ccgcaccttc gtggcgcgcg
    10141 aatcggctcc ggcagcgccg gttcgtggcg gcatcctcgc cggcatcctc ggcttcgcgc
    10201 tggcggcgtg cggtggcggc ggcggtcgcg gcgcggcacc gatcaccccg ccgccgacca
    10261 ccccgccgcc cgcagcgacg atcagcgagg ccgaggccgc caggttcctc atccaggcca
    10321 gcttcgggcc gacccgcgcg gagatcgccc gggtgcggga gctcggctat tcgcgatggc
    10381 tggacgagca gatggatccg gcgcgcacgc cgccgacgct ggtgttgccg cacatccagc
    10441 agatcctcgc caacggcctg gccgaaaccg acctcaccca ggcccaccgc cgcaatgcct
    10501 ggctttggtt cgccgcgaac aaccgcgacc agttgcgcat gcgcatggcg ttcgcgctca
    10561 gcgagatctt cgtggtctcc gacttcgaga gtgggcgcgc gcagatcccg cgggtggccg
    10621 attaccagga cacgctggcg cgcaacgcgt tcggcagcta ccgcagcgtg ctgaaggcgg
    10681 tcaccctgca tccggcgatg ggcgtgtacc tgagccatgc cggcaaccgc aaggccagtg
    10741 cggacggcaa gatcgtgccg gacgagaact acgggcgcga ggtgatgcag ttgttcagca
    10801 tcggcctggt cgagcgcaac caggatttct cgccggtgct ggatggctcc gggcagacgg
    10861 tgccgaccta cgattcggcg gttgtcgcgg cgatgtcgcg ggtgttcacc ggctggacct
    10921 acgcaggcca gacggatgcc aactatggcc gcgtggacgg gcgcagctac gccccgatgg
    10981 agtgccaccc cgcctaccat gacgaccagc ccaaggcgat cttccgtggc atcgtgatcg
    11041 ccaacggcaa cgattgcgcc gcaaccctgg aacgcgcgct ggatgccctc agccagcatc
    11101 cgaacaccgc gcccttcatc agccgccagc tgatccagcg cttcgtcacc agcaatccga
    11161 gcccgggcta catccagcgc gtgggccagg cctggagcac gagcggggga gacctcggcc
    11221 gcgtgctgcg cgcgatcctg ctcgacggcg aggcgcggac cgcccccgcc gccggcgacg
    11281 ccaccttcgg caagccgagg gagccgctgc tgcggatcgc ccacctgtgg cgcgcgtacg
    11341 aagcgaggta cgtgccgcct gcgagcggcg cgctgcgctt ccgcgccggc agcaccgcgg
    11401 acctcgccct gtcgatcgcg caggactcgc agcgcgcgcc atcggtgttc aacttcttcg
    11461 agcccgacta ccggatcccg gcctccgacg gcagccaggg ggtgtacgcg cccgagctcc
    11521 agatcgtcaa cgagtcgacc ttcaccaccg gccacaacca gagcgatgcc ctgctgtgga
    11581 actacgccag cacctccgcg cctgccgcca acaccaatgc gccggtgctc gacatcaccc
    11641 gcctggtgca actcgcggag gccggcgacg acgccgcgat ggtgcaggac gtgaacctgc
    11701 tgctgcacgg tggcagcctg ccggcggcca gcgcggccac cctgacgcgc atgctgggca
    11761 gcctgcgcgc gcagggtcgc ggcgcgtcgg agcgcgcccg ctcgttgttg ctggtcgcgc
    11821 tgctgtcgcc ggactacgcg gtccagcgct gattcgaacc cgcacgccgc atcctcgcgc
    11881 cacgcaagga acaaccccat gccgcactcg acccgacacg accgccgccg cttcctgcaa
    11941 gggctgggtg ccctggtcgc gaccggaacc gccggagccc tgtttcccca gctcgagctg
    12001 atggggcacg cgcttgcggc gacgccccag gccggggact accgcgccct ggtctgcatc
    12061 ttcctgtacg gcggcaacga ttcgtacaac atgctgatcc cgcatgcgca ggcggagtac
    12121 gacgtctacc tgcgcagccg tggcggggtc cacgacgcca cctccaatcc gttcgggctg
    12181 ggcatcgcgc gcgactcgct ggccaccatc gccgacacct ccggcggtac ctggggcctg
    12241 catccggcgt tcgccgcggc caagccgctg ttcgatgccg gcgagctggc gttcctcgcc
    12301 aatgtcggca gcctggtcga gccggtgcgc aagtcggaag tcggctatcc cggcggcaag
    12361 ccgctgccgc cctacctgta ctcgcacaac gaccagcagc gccagtggat gcgcgggcac
    12421 gccaccacca cccacgccgc caacggctgg ggcggcctgt gcggcgatcg cctggcggcg
    12481 ctcaacaccg gcctgtcggc gctgccgccg accatttcga tcttcggcaa caacctgtac
    12541 cagagcggcg cctcggtact gccgtatgcg gtcgcctcga acgggccggg cgaactggcg
    12601 cgcatggcaa gcaacggcgg cagtgccgac gcgatccgcc tgcaggccct cgaggaactg
    12661 ctgcacgcca accagggcca tgcgatgcgg acgcgctacg ccggcctggg cgagacctcg
    12721 atcatggtca acggcgcgtt gcggcaggcg ctggatccgg ccaatggcgg cgacatcgcc
    12781 accaccttcc cgggcgagcc gctggccgcg cagctgcgga tgatcgcgcg cctggtcaag
    12841 gtcagccagg ggccggcgat cggccacaag cggcagatct tctacgccgg gctgggcggc
    12901 ttcgataccc acgacaacca gatggacccg acccggcacg cggcgctgct gtcgcaggtg
    12961 gcgggcgcgc ttgccgcgtt ccgcgccgcg ctgcaggaga tcggcatgct cgaccgcgtg
    13021 accaccttca ccatgagcga tttcgggcgc accctcaaca gcaacggcaa tggcaccgac
    13081 catgcatggg gcggcgtgca gctggtgatg ggcgccgccg caggcaatgg cggctcgctg
    13141 caggggcgcc gggtctgggg cgactatccg ctgctggaac tggatggcga gcaatcgatg
    13201 gggcgcggcc ggatgatccc gaccacctcg atccagcagg tcggcgcgac cctggcgagc
    13261 tggatgggcg ttgccgaggc cgacctcggg acgatcttcc ccgggatcgg caacttcgat
    13321 gcgcccaggc ttggcttcct cggctgaccg cgatcagcgg tagcggttga tctcgtcgcg
    13381 caggctgcgc gcggcatccg cggcggcatc ggcgtaaccg gcgccggagg atgcgtacag
    13441 gatcccgcgc gaggaattga tcaccaggcc ggtgccatcg gcggtcttgc cgttgcgcac
    13501 ggtggcttcc acgtcgccgc cctgcgcgcc gatgccgggg atcagcaggg gcatgtcgcc
    13561 gacgatgccg cggatcacct tcaactcttc cgggaaggtg gcgccgacga ccagcgcgca
    13621 gttgcggtcc gcgttccact cgccggcgat cgtccgcgcg atgcgctggt agagcggctc
    13681 gccgccgacg tcgagttcct ggaaatcgca tgcgcccggg tttgaggtgt ggcacaggaa
    13741 cacgcacccg cggtcgttgt actgcaggaa cggatcggcg gaatccctgc ccatgtacgg
    13801 gttcagcgtc accgcgtcgg caccgtagcg ctcgaacgct tccaccgcgt actgcgccgc
    13861 ggtgctgccg atgtcgccgc gcttggcgtc caggatcacc gggatatcgg ggaagcagcc
    13921 gttgatgtgc gcgatcagcc gctgcagctg cgcctcgccg tcgttgtgcg cggcgaagta
    13981 ggcgatctgc ggcttgaacg cgcaggcgaa cccggcggtg gcgtcgacga tgtcgcggca
    14041 gaacgagaac agcgcgtcct cgtcgtcgat gaaggcctcc gggaacctcg ccgggtcggg
    14101 atccaggcca acgcagacca gcgagttggc cgagtgccag cggttgcgga gtttttcgat
    14161 gaaggtcatg gcctgcttcg ctcctgctcg tcattcccgc gcaggcggga tgacgatttg
    14221 gcaagggcgt gcgcaatgcg cacgcgccaa atcgtcactt cagcgccttg aaccgcaggc
    14281 ggtgcggctg cgcaccggct tcgccgaggc ggcgcttctt gtcttcctcg tactcgcggt
    14341 agttgccctg gaagaactcc acgtgcgagt cgccctcgaa cgcgaggatg tgggtggcga
    14401 tgcggtccag gaaccagcgg tcatgcgaga tcacgaaggt gttgccgggg aactccagca
    14461 gcgcgtcttc gagcgcgcgc agggtttcga tgtcgaggtc gttggacggt tcgtcgagca
    14521 gcagcacgtt gccgccctgc agcagggtct tggccatgtg caggcggccg cgctcgccgc
    14581 cggacagggt gccgaccagc ttctgctggt cctggccctt gaagttgaag cggccgatgt
    14641 aggcgcgcga ctggatctcc accccgttga tgttgaggat gtccaggccg ccggacacct
    14701 cctggaacac gttgtggttg ccttccagct tgtcgcggct ctggtccacg taggccagct
    14761 tgacggtcgg gcccatctcg atgctgccgg tgtccggctt ttcctggccg gtgatcatcc
    14821 ggaacagggt cgacttgccg gcgccgttgg gaccgatgat gccgacgatg gcgccgttcg
    14881 gcacgctgaa gctgaggtcg tcgatca
//
LOCUS       DAORUK010000044        14870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_3830_length_14870_cov_17.317246, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000044 DAORUK010000000
VERSION     DAORUK010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 14870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14870
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3830_length_14870_cov_17.317246"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PK818_04545"
     CDS             <1..452
                     /locus_tag="PK818_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HQY82039.1"
                     /translation="RSIRYMLDLSDNKVIEVIQLADPGFAIDKDDVRAFLLREDEPGY
                     QACTDRVMAHFLDGLVIHRRGRDESLPPRPVEKRINNNVVLKKLRVAFQLTDVDMHKA
                     FADAGFPVSRPELSALFRQPGHKNFRLCGDQLLRNFLKGLTLRVRGR"
     gene            573..1343
                     /locus_tag="PK818_04550"
     CDS             573..1343
                     /locus_tag="PK818_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82040.1"
                     /translation="MKRALVSVLVLASLAPIAAMAQDGPSFSLFAGRDMSVNGDVHTG
                     AIAPIADLGPLNPALAGVSAELRIESRSYDEIYGNANSFGIEMSWPMSSGEVFGQLRT
                     SSSSDGEVQVGGAYVPALSTTLPVYGRFEGYRSLSLEGGYRHYFATGKVRPYLAGRVG
                     TTRVDDISATFEIPDADILLSDVPFFEAGWNFSGGADVGVMWALSDAASLGLEVGARY
                     HGDLDGDDSAIGGLGLASINDTGSRTSFPISLRFQMKF"
     gene            complement(1404..2762)
                     /locus_tag="PK818_04555"
     CDS             complement(1404..2762)
                     /locus_tag="PK818_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HQY82041.1"
                     /translation="MTDNAQPGHPPGKLLQVLGVAFGLAVLVGNTIGMGILRTPGEIA
                     AQVPSVPWFMGVWVAGALYALLGALTVSELVAMRPRSGGLYPLVRHGLGPYPGFVVGW
                     SDWLSTSGSIAAVAIVLGEYAGPLVPALAGREKAIACTVVFGFGLLQWRGIRLGDLAQ
                     QATSLAKALALVGLALFALLLAKPQAPATVATAAPLLPPGLALVGAVIVAMQSAIYTY
                     DGWTGPVYFSEELADPARDVPRTMIGGVLLVMTIYLLLNAAFLHVIPIREMAGDPFVA
                     ATAAARMFGPAGDTVLRIVMVLSLVASVNALVLMASRVPFAMSRDRLLPAILQRVNAG
                     GTPVPALWASIALALGLILTNTFASVVAMLAFMFVANYAVTFATFFVMRRRQPQAPRP
                     FRVPLYPLVPALALLGSLAFIAASLASDTRNSLYALLLVAVSWPVYRLFRRGPAAAQA
                     QD"
     gene            3048..4235
                     /locus_tag="PK818_04560"
     CDS             3048..4235
                     /locus_tag="PK818_04560"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HQY82042.1"
                     /translation="MARALLVVVDSLGIGGAADAGRFGDTGADTFGHILQARAPALPN
                     LRALGLHAAHALAHGRAPATDERADPASTWAAMQEHSTGKDTTSGHWEMCGVPVEFDW
                     GYFTAPEHSFPPDLLDALCSRAGLAGVLGNCRASGTEIIERLGREHLASGKPIVYTSA
                     DSVLQIAAHEQHFGLERLYATCRIARELVDDYRIARVIARPFAGDAVDGFRRTGNRHD
                     YSVPPTAPTLLDALVAAGHQVLAIGKVSDIFAGQGISRAIPASGLPALLDATEATFAD
                     APDGSLVFVNLVDFDSEYGHRRDVAGYAAALEYFDARLPALLRLAQAGDLVLLTADHG
                     NDPTWPGSDHTRECVPLLAVGGGLRTGAAGLRDGFADLGQTLARHFGLPPLRHGVALP
                     LSS"
     gene            4244..4975
                     /gene="deoD"
                     /locus_tag="PK818_04565"
     CDS             4244..4975
                     /gene="deoD"
                     /locus_tag="PK818_04565"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HQY82043.1"
                     /translation="MPTPHISAAPGAFADTVLLPGDPLRAEHIARTLLDDAVEVTRVR
                     AMAGFTGSWNGRPVSVMGTGMGIPSSSIYATELVREYGVRRLLRIGTCGAVSESLRLG
                     DIVLGQGACTDSGVNRSRFRGMDFAAIAGFDLLAAVAATARERRVPVAIGNLFSADLF
                     YHPEPTLVDALDAMGVLGIEMEAAGLYGIAAEHRVQALAVCAVSDHLRSHASWSPQQR
                     QCGVDDMVRLVLDAVIGAPPPGVDA"
     gene            5114..6184
                     /locus_tag="PK818_04570"
     CDS             5114..6184
                     /locus_tag="PK818_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQY82044.1"
                     /translation="MTASEPSPPVREDSSDWDSRSHVHALVMMAATVVGLYLCYRMAA
                     PFVPALAWALALAILLAPLQRRLEPKLRSPGLAAGVLVLLVGVVLVLPAMLVADHLLD
                     EAYRGADALTAMVETGQWRRSLEAHPLLAPVADWVERQFDLPDTVDAVTSWLTGAMAS
                     VARESLLQLVGMVLTLYMLFYFLRDRRGILAAIESHLPLAKADAARMFGDIDDTVHAT
                     VYGTLIVAMVQGILGGLMFWWLGLPAPLLWGVLMGLLAVVPVLGAFIIWIPAAVFLLL
                     DGSGGKALLLTVWGSVVVGGIDNVLYPMLVGRRLKLHTLLAFVSIVGGLVVFGSSGLI
                     LGPVVFAITRLLLQVWNRRHAA"
     gene            6282..6614
                     /locus_tag="PK818_04575"
     CDS             6282..6614
                     /locus_tag="PK818_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HQY82045.1"
                     /translation="MPVRPLSASGRLYAIAAFVEALTWAGLLVGMWLKYGANVTDAGV
                     WLFGRLHGAAFLFYMVATMITASRQRWPGWALLLGLLAAIPPLVTLPLEWWFRRRGLL
                     SPLPEAHQ"
     gene            6611..7384
                     /locus_tag="PK818_04580"
     CDS             6611..7384
                     /locus_tag="PK818_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY82046.1"
                     /translation="MNAPVQGLQDPAQVLVTGASSGIGLAMVQALLEDAAVARVYAVS
                     RRGGACEVLQALRERHGERLQAVAADLTCDTGLADVAAAAASGGALHLVVNCAGILHG
                     AGLRPEKSLATLQRDALAQVFALNAFAPVLLAKALLPLLGPGAPRVFASLSARVGSIS
                     DNRLGGWYAYRASKAAQNQLLRTLAIEWRRTHPHATCVLLHPGTVDTPLSRPFQANVP
                     PAALSTPGHAARRLLDVIAGLTPADSGRFIAWDGSPVPW"
     gene            7464..8003
                     /locus_tag="PK818_04585"
     CDS             7464..8003
                     /locus_tag="PK818_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HQY82047.1"
                     /translation="MNNPLKPRYTPALRRLHWLMAILIVAAYLLIEQRGVFPRGSAER
                     AAMVQGHFWVGITVFVLAWWRLARRRIDGAPGVTPPLGRATAIVSKLFHLALYAFFLV
                     MPVLGMATAWTDGKAVLVPFTGIALPALLAENEDLAHTLEDLHGSIGEAFYWVIGLHV
                     LAALWHHFGRRDDTLRRML"
     gene            8054..8269
                     /locus_tag="PK818_04590"
     CDS             8054..8269
                     /locus_tag="PK818_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82048.1"
                     /translation="MPRKPVDPSSLPPWKRKSPPGKSRPLSAVQVEAARRRAAQAGRR
                     YPNLVDNMWAARHVPRGDAVACSGEDE"
     gene            complement(8273..8929)
                     /locus_tag="PK818_04595"
     CDS             complement(8273..8929)
                     /locus_tag="PK818_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HQY82049.1"
                     /translation="MTGATGLVGQGVLREVLADPRVTRVGVLGRRGTGNDDPRVEDLQ
                     VERFDALDGVAGRLAPWDACFYCAGAPPLGTPEAEYRHVTVALTLQVARVFAERNPGG
                     RFLYVSGAHANPGSRLMPLRVKGEAEAALRALPITTVMLRPGGIQPAHGERSPHAWMR
                     PLYALGAPLLGLGVRLLPGVVTSTAAVGRAMLVLAAMADAPAVVENDGINRLAAAARD
                     "
     gene            9088..9270
                     /locus_tag="PK818_04600"
     CDS             9088..9270
                     /locus_tag="PK818_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HQY82050.1"
                     /translation="MKANVGGIDRWIRIVLGLALIAWAATGGPAWAWIGAVPLATGVF
                     NFCLLYRLLGINTCHR"
     gene            9402..9980
                     /locus_tag="PK818_04605"
     CDS             9402..9980
                     /locus_tag="PK818_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82051.1"
                     /translation="MTGGSAIEKGPRGNRLRPFVWGAAACLLLLPAVAMQWFPASEVN
                     WTALDFIAMGVLLAILCGLYELGAWMSDNIAYRAGFGLAALTGFLTIWVNLAVGMLGD
                     EDGTINLVFAGVLFIATAGALIAGLRPRGMALAMLAAAIAQLAAVGVALAMGGYDARE
                     LTFTAMFGLPWLASAALFRKAAADARGLPGAC"
     gene            10065..10958
                     /locus_tag="PK818_04610"
     CDS             10065..10958
                     /locus_tag="PK818_04610"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008207817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQY82052.1"
                     /translation="MLALRLVLLFLLIAANTLLHVPPLLVVALFKAVLPFQRVRVASN
                     PVLTGLAESWIGVNSWMWDRFTGTRLQLDEDVALQADGHYLVLANHQSWVDILVLQKV
                     FNRRIPLLRFFLKRQLFWVPLLGLAWWALDFPFMGRYTPKQVARRPELARRDIEATRR
                     ACGKFSAIPVSIMNFVEGTRFTVAKHAAQGAPYQHLLKPKSGGVAFVLDAMGAGLHAI
                     LDVTIVYPAGRPSLLDLLANRIPAVHVQVRQREIPPALIHGDYQGDRGFRAGFQQWIN
                     GIWREKDADVATMLAGSAHRA"
     gene            complement(11013..11195)
                     /locus_tag="PK818_04615"
     CDS             complement(11013..11195)
                     /locus_tag="PK818_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82053.1"
                     /translation="MFSPRPAAKNPGMTHERIAADLDAFHKAGGKIEVLGVTRSLKKI
                     DGDDGSPPPAPAKPRR"
     gene            complement(11286..11627)
                     /locus_tag="PK818_04620"
     CDS             complement(11286..11627)
                     /locus_tag="PK818_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HQY82054.1"
                     /translation="MPATDRRVTASATPTEAPHCDRCDAVCCRLTVVLMPEDDVPLRF
                     TARNEHGVEVMARDEDGWCVAIDQARMCCSIYELRPTICRKFPMGGPYCRDVRADYAD
                     RNARGIPLTLY"
     gene            complement(11603..11806)
                     /locus_tag="PK818_04625"
     CDS             complement(11603..11806)
                     /locus_tag="PK818_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82055.1"
                     /translation="MPGYPIRETEHPIDGHRDRLRLRNDTRRFADPDGRRARLGILLA
                     HRSGSGRVRPCGCLLHCRRRIVA"
     gene            12016..12288
                     /locus_tag="PK818_04630"
     CDS             12016..12288
                     /locus_tag="PK818_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82056.1"
                     /translation="MSQHQIALFTFTLGNTESISPVALRDLWVRAAGIGNVSVGRKSP
                     HGSYRDRPTYMLYAPQSLGNLRDVELRLRKLLEDAHLNASLTPLHA"
     gene            complement(12368..13090)
                     /locus_tag="PK818_04635"
     CDS             complement(12368..13090)
                     /locus_tag="PK818_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HQY82057.1"
                     /translation="MSEPVRLAKRVAELARCSRIAAEQYIKCGWVRVDGRVVEIPHLL
                     VTDEAVSIDADALLEAVEPATLLLHKPAGFDTISGANPATALVAPETHWTEDPSGVRL
                     LERHFQRLTPLVPLDRDASGLMVLTQDGRIWRRLTEDQDLIEQEFVVEVSGEIAPYGL
                     RKLNHGLHYNGRPLPPCKVSWQNEVRLRFALKGVQGGQLRDMCKQVGLDVVAIRRLRI
                     GKVSLAKMPVGQWRYLPVGDRF"
     gene            13211..14068
                     /locus_tag="PK818_04640"
     CDS             13211..14068
                     /locus_tag="PK818_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HQY82058.1"
                     /translation="MKTPSGLQPLIDDGVIDEVMRPLKSGKEAAVYVVRAGNEVRCAK
                     VYKDMAQRSFQQRVQYQEGRKVRGSREARAIGKASKYGRKQQEAAWKNTEVDALYQLR
                     DAGVRVPEPFGYFHGVLVMELVVDADGHSAPRLGEVELSPEQARGFHRFLVRQVIRML
                     CVGLIHGDLSEYNVLVAPDGPVIIDFPQVVSAAGNNAARTMLLRDVNNLTASLGGWAP
                     ELLETWYGEEMWALFEAGELHPDSELTGAFVHDESTPDIDGVRHAINDAREEALIRQQ
                     GREAAAQDD"
     gene            complement(14090..>14870)
                     /locus_tag="PK818_04645"
     CDS             complement(14090..>14870)
                     /locus_tag="PK818_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQY82059.1"
                     /translation="ARDAGEPAWSGSLDLGRSSAAAALRADGWEWRGQHYPYPGTLKE
                     RTIYWWDGDGFAAVSRYAGSLIKLVPTEWDAPTFEIDGIKMLPTSKESPVDDARRKVA
                     LVQPAGMRVLDTCGGLGYFAACCLDAGVASIQSFEKNEDVLWLRALNPWSPDPATSGG
                     RLQLAHADVSKAIVALPDAGFDAVLHDPPRFGIAGELYSQAFYDQLARVIKRGGRLFH
                     YTGTPNRLTSGRDLPREVARRLEKSGFRAGPALDGVLATRR"
BASE COUNT         2127 a         5138 c         5320 g         2285 t
ORIGIN      
        1 tgcgcagcat ccgctacatg ctggatctca gcgacaacaa ggtgatcgag gtcatccagc
       61 tggcggatcc cggcttcgcc atcgacaagg acgacgtgcg cgcgttcctg ttgcgcgagg
      121 acgagccggg ttaccaggcc tgtaccgacc gggtgatggc gcatttcctc gacgggctgg
      181 tcatccaccg ccgcggccgc gacgaatccc tgccgccacg tccggtggaa aagcggatca
      241 acaacaacgt ggtgctgaag aagctgcggg tggcgttcca gctcaccgac gtggacatgc
      301 acaaggcgtt cgcggatgca ggcttcccgg tctccaggcc ggagctgtcg gcactgttcc
      361 gccagcccgg gcacaagaat ttccgcctgt gcggggacca gttgctgcgc aatttcctga
      421 agggcctgac cttgcgggta cgcggccgct aggactgcgg gtggggggcg tgcacgcggg
      481 tgcatccgat tggcgtgcgg tcgcgtaagg gagtctgtcg gctactgccg gcccgtattc
      541 cccctcaacg accagcagtc aggagacctt cgatgaagcg tgccctcgtt tcggtactcg
      601 tccttgccag ccttgccccc atcgccgcga tggcccagga cggccccagc ttttccctgt
      661 tcgccgggcg cgacatgtcc gtcaatggcg acgtccacac gggcgcgatc gcgccgatcg
      721 ccgacctcgg cccgctcaat ccggcgctgg ccggcgtctc ggcggaattg cgcatcgagt
      781 cgcggtccta cgatgaaatc tacggcaatg cgaacagctt cggcatcgag atgagctggc
      841 cgatgtccag cggcgaggtg ttcggccagt tgcgcacctc cagctccagc gacggcgagg
      901 tccaggtcgg cggcgcctat gtgcccgcgc tcagcaccac gctgccggtt tacgggcgct
      961 tcgagggcta ccgcagcctg tcgctcgagg gcggctaccg gcactacttc gcgaccggca
     1021 aggtgcgtcc gtacctggcc gggcgggtcg gcacgacccg cgtcgacgac atctccgcca
     1081 ccttcgagat cccggatgcc gacatcctgc tcagcgacgt accgttcttc gaggctggct
     1141 ggaacttcag cggcggcgcc gacgttggcg tgatgtgggc gctttccgat gcggcatcgc
     1201 tgggcctgga ggtcggcgcg cgctaccacg gtgacctcga cggcgacgac agcgcgatcg
     1261 gcgggcttgg cctggcctcg atcaacgaca ccggttcgcg cacctcgttc ccgatcagcc
     1321 tgcgcttcca gatgaagttc tgagtcctgc gccccggggc ccggatcggc cgggtcccgg
     1381 ggcgttcgcg gcggaacgcg tggtcagtcc tgcgcttgcg ccgcggcggg tccacgccgg
     1441 aacagccggt acaccggcca cgaaacggcg accagcagca gggcgtacag gctgttgcgg
     1501 gtatcgctgg ccagcgacgc ggcgatgaag gccagcgaac cgagcagggc gagcgccggc
     1561 accagcgggt acagcggtac ccggaacggc cgcggcgcct gcggctgccg ccggcgcatg
     1621 acgaagaagg tggcgaaggt caccgcgtag ttggccacga acatgaaggc cagcatggca
     1681 accacgctgg cgaaggtgtt ggtcaggatc agccccagcg ccagcgcgat gctggcccac
     1741 agcgcgggca ccggggtgcc gccggcgttc acccgctgca ggatggccgg cagcaggcgg
     1801 tcgcggctca tcgcgaacgg cacccgcgac gccatcagca ccagcgcgtt cacgctggcg
     1861 accagcgaaa ggaccatcac gatgcgcagc acggtgtcgc cggccgggcc gaacatccgc
     1921 gccgccgcgg tggcggccac gaacgggtcg ccggccattt cccggatcgg gatcacgtgc
     1981 aggaacgcgg cgttcagcag caggtagatc gtcatcacca gcagcacccc gccgatcatc
     2041 gtgcgcggga catcgcgcgc cgggtccgcg agttcctcgc tgaagtagac cggcccggtc
     2101 cagccgtcat aggtgtagat cgccgattgc atcgccacga tcaccgcgcc gaccaatgcc
     2161 agccccggcg gcagcagcgg cgccgcggtg gccaccgtgg ccggtgcctg cggcttcgcc
     2221 agcagcagcg cgaacagcgc cagtccgacc agcgccagcg ccttggccag gctggtcgcc
     2281 tgctgggcga ggtcgccgag acggatcccg cgccactgca gcaggccaaa gccgaacacc
     2341 acggtgcagg cgatcgcctt ttcgcggccg gccagggccg gcaccagcgg acctgcgtac
     2401 tcacccagca cgatcgccac cgcggcgatg ctgccgctgg tcgaaagcca gtcgctccag
     2461 cccaccacga acccgggata cggtccgagc ccgtgccgaa ccagcggata caacccgccg
     2521 gagcgcggac gcatcgccac cagttccgaa accgtcagcg cacccaacag ggcataaagc
     2581 gcgccggcca cccacacacc catgaaccag gggaccgagg gcacctgcgc ggcgatctcg
     2641 ccaggcgtac gcaggatgcc cattccgatc gtgttgccga ccagcaccgc caacccgaag
     2701 gcgacgccga ggacctgcag cagcttgcct ggcgggtggc cgggttgcgc gttgtcggtc
     2761 atggaacccc cgggttgcgt tgcgattgca cgccaatgca gcgatgggtg ccggggcagg
     2821 gtgcgccagt tgcagcgccc gcgccagcgg cacgcctgcg cggcgccgca gcggcgcgcc
     2881 cgcactgcct tgcgccgcgc ggccacgctt gccctggatc cgtgcatcag ccgccgcgcg
     2941 tggctaggat ggcgcatcac tgcacaccat ccgaccatga ccgaccgcct gctccctgcg
     3001 tgcctgctcg atcctgccag cctgggcgcc gccggagcat gccgctgatg gcgcgcgcat
     3061 tgctggtggt ggtggattcg ctggggatcg gcggtgccgc cgatgccggg cgcttcggcg
     3121 acaccggtgc cgacaccttc ggccacatcc tgcaggcgcg ggcgccggcg ctgccgaacc
     3181 tgcgcgcgct gggcctgcac gccgcgcacg cgctggcgca cggacgcgca ccggcgacgg
     3241 acgaacgcgc ggatcccgcg tccacctggg cggcgatgca ggaacactcc accggcaagg
     3301 acaccaccag cggccattgg gaaatgtgcg gcgtgccggt cgagttcgac tggggctact
     3361 tcaccgcgcc cgagcacagc ttcccgccgg acttgctgga cgcactgtgc agccgcgccg
     3421 ggctggctgg cgtgcttgga aactgccgcg cctcgggcac cgagatcatc gaacgcctcg
     3481 gccgcgaaca cctggccagc ggcaagccga tcgtctacac ctcggccgat tcggtgctgc
     3541 agatcgccgc gcacgaacag cacttcggcc tggagcggct gtatgcgacc tgccggatcg
     3601 cgcgcgaact ggtcgacgat taccggatcg cgcgggtgat cgcgcggccg ttcgcgggcg
     3661 atgccgtcga cggcttccgc cgcaccggca accgccacga ctactcggtg ccgccgaccg
     3721 cgccgaccct gctggacgcg ctggtcgccg ccggccacca ggtactggcg atcggcaagg
     3781 tctccgacat cttcgccggc cagggcatca gccgcgcgat cccggccagc ggcctgccgg
     3841 ccctgctgga cgcgaccgag gccacctttg ccgacgcgcc tgacggcagc ctggtgttcg
     3901 tcaacctggt cgatttcgat tccgagtacg gccaccgccg cgatgtcgcc ggctacgccg
     3961 ccgcgctcga atacttcgat gcgcggctgc cggcattgct gcggctggcg caggccggcg
     4021 acctggtgct gctgaccgcc gaccatggca acgacccgac ctggcccggc tccgaccaca
     4081 cccgcgaatg cgtgccgctg ctggccgttg gcggcggcct gcgcaccggc gccgccggcc
     4141 tgcgcgatgg cttcgccgac cttggccaga ccctggcccg ccatttcggg ctgccgccgc
     4201 tgcgccacgg cgtggcgctc ccgctatcca gctgaggacc acgatgccga ccccgcacat
     4261 ctccgccgcg cccggcgcct ttgccgacac cgtgctgttg ccgggggatc cgctgcgcgc
     4321 cgagcacatc gcgcgaaccc tgctggacga cgcggtggaa gtcacccgcg tgcgtgcgat
     4381 ggccggcttc accggcagct ggaacggccg gccggtctcg gtgatgggca ctggcatggg
     4441 catcccgtcg tcgtcgatct acgccaccga gctggtccgc gaatacggcg tccgccggct
     4501 gctgcggatc ggtacctgcg gcgccgtgtc ggagtcgctg cggctgggcg acatcgtgct
     4561 ggggcagggc gcctgcaccg attccggggt caaccgcagc cgcttccgcg gcatggattt
     4621 cgccgcgatc gccggcttcg acctgctggc ggcggtggcg gccaccgcgc gcgagcgcag
     4681 ggtgccggtg gcgatcggca acctgttcag cgccgacctg ttctaccacc cggaaccgac
     4741 cctggtcgac gcgctggatg cgatgggcgt gctcggcatc gagatggaag cggccgggct
     4801 gtacggaatc gccgccgagc accgggtgca ggcactggcg gtgtgcgcgg tctccgacca
     4861 cttgcgcagc catgccagct ggtcgccgca acagcgccag tgcggcgtcg acgacatggt
     4921 ccggctggtg ctggatgcgg tgatcggcgc gccgccgccg ggtgtcgacg cctgaccgtg
     4981 gcgttggccg ccagcggcac ctggaaagcc ccggtcctgg acgccggacc gggtccggcg
     5041 cgcagcgtgc acggcgcggc tggccgcaag cgtgcacact gatgcggtcc cgcttcccga
     5101 cgccatgacc gacatgaccg catccgagcc gtcgcccccc gtccgcgaag acagcagcga
     5161 ctgggattcg cgcagccacg tgcatgcgct ggtgatgatg gcggcgacgg tggtcgggct
     5221 ctacctgtgc taccggatgg cggcgccatt cgtgcctgcg ctggcatggg cgctggcact
     5281 ggcgatcctg ctggcgccgc tgcagcgccg gctggagccg aagctgcggt cgcccgggct
     5341 tgccgcgggg gtgctggtcc tgctggttgg cgtggtcctg gtgctgccgg cgatgctggt
     5401 cgccgaccac ctgctcgacg aagcctatcg tggcgccgac gcgctcaccg cgatggtcga
     5461 aaccgggcag tggcggcgca gcctggaggc gcacccgctg ctcgccccgg tggccgactg
     5521 ggtggagcgc cagttcgacc tgccggatac cgtcgacgcg gtcacctcgt ggctgaccgg
     5581 cgcgatggcc tcggtggcgc gcgagtcgct gctgcagctg gtcggcatgg tgctgaccct
     5641 gtacatgctg ttctatttcc tgcgcgaccg gcgcggcatc ctcgctgcga tcgaatcgca
     5701 cttgccgctg gccaaggccg atgccgcgcg catgttcggc gacatcgacg acaccgtgca
     5761 cgcgaccgtg tacggcaccc tgatcgtggc gatggtgcag ggcatcctcg gcgggttgat
     5821 gttctggtgg ctggggctgc cggcgccgtt gctgtggggc gtgctgatgg gcttgctggc
     5881 ggtggtgccg gtgctcgggg ccttcatcat ctggatcccg gcggcagtgt tcctgctgct
     5941 ggacggcagc ggcggaaagg ccctgctgct caccgtgtgg gggtcggtcg tggtcggtgg
     6001 catcgacaac gtgctgtatc cgatgctggt gggcaggcgc ctgaagctgc atacgctgct
     6061 ggccttcgtc tcgatcgtcg gtggcctggt ggtgttcggg tcgtcgggat tgatcctggg
     6121 accggtggtg ttcgccatca cccgcctgct gctgcaggtc tggaaccggc gccacgcggc
     6181 ctgagcgctg caggtgccgg cgggtggccg gcgcgcgcca gcagcaccac gccggctgcc
     6241 gttttgggga caatgctccg ccatcccgca cggaatccac gatgcctgtc cgacccctgt
     6301 ccgcgagcgg acgcctgtac gccattgcag cgttcgtcga ggccctgacc tgggccgggc
     6361 tgctggtcgg gatgtggctg aagtacgggg ccaacgtcac cgatgccggc gtctggctgt
     6421 tcggccgcct gcatggcgcg gcattcctgt tctacatggt ggccaccatg atcaccgcca
     6481 gccgccagcg ctggccgggc tgggcgttgc tgctgggcct gctggcggcg ataccgccgc
     6541 tggtgacgct gccgctggag tggtggttcc ggcgcagggg cctgctctcg ccgctcccgg
     6601 aggcgcacca atgaacgcgc cggtccaagg cctgcaggat ccggcacagg tgctggtgac
     6661 cggtgccagc agcgggattg gcctggcgat ggtgcaggcg ctgctggagg acgccgcggt
     6721 ggcgcgggtg tacgcggtgt cgcggcgtgg cggtgcatgc gaggtgctgc aggcgttgcg
     6781 ggaacgtcac ggcgaacggc tgcaggcggt cgccgccgac ctcacctgcg acaccggcct
     6841 tgcggacgtg gccgcggcgg cggcgtccgg cggcgcgctg cacctggtgg tcaactgcgc
     6901 gggcatcctg catggcgcag gactgcgccc ggagaaatcg ctggcgacgc tgcagcggga
     6961 tgcgctggcg caggtgttcg cgctcaatgc gttcgccccg gtgttgctgg cgaaggcgtt
     7021 gctgccgctg ctggggccgg gcgcgccgcg ggtgttcgcc tcgctgtcgg cgcgggtcgg
     7081 ttcgatctcc gacaaccggt tgggcggctg gtatgcctac cgcgcctcga aggcggcgca
     7141 gaaccagttg ctgcgcacgc tggcgatcga gtggcggcgc acgcatccgc atgcgacctg
     7201 cgtgctgctg catccgggca cggtcgacac cccgctgtcg cgaccgttcc aggccaacgt
     7261 gccgccggcg gcattgtcca cgccgggaca cgccgcgcgc cgcttgctgg acgtcatcgc
     7321 cggcctgacc ccggccgaca gcggccgctt catcgcctgg gacggcagcc cggtgccatg
     7381 gtgagcgcgg ccgccgcgat cacgcggtac accgatgccg cactcaggcg ttatcgttgg
     7441 ccaacaccca ccggagactg cccgtgaaca atccgctgaa gccacgctat accccggcac
     7501 tcaggcgcct gcactggctg atggcgatcc tgatcgtggc cgcctacctg ctgatcgagc
     7561 agcgcggcgt gttcccgcgc ggcagcgcgg agcgcgcggc gatggtgcag ggccacttct
     7621 gggtcggcat cacggtgttc gtgctggcct ggtggcggct ggcccggcgc cggatcgatg
     7681 gcgcgcccgg cgtcaccccg ccgctggggc gggcgacggc gattgtctcc aagctgttcc
     7741 acctggcgct gtacgcgttc ttcctggtga tgccggtgct cggcatggcc accgcgtgga
     7801 ccgatggcaa ggcggtgctg gtcccgttca ccgggatcgc gctaccggcg ctgctggccg
     7861 agaacgagga cctcgcgcac accctggagg acctgcacgg cagcatcggc gaggccttct
     7921 actgggtgat cggtttgcac gtcctggccg cgctgtggca ccacttcggt cgccgcgacg
     7981 acaccctgcg ccggatgctg taaccgcgac ctttcagcga acctcgcggc gtgcatccgg
     8041 ctaccatccg cggatgccgc gcaaacccgt cgatccctcc agcctgccgc cgtggaaacg
     8101 caagtcgcca cccggcaagt cgcgcccgct gagtgccgtg caggtcgagg cggcacggcg
     8161 ccgggcggcg caggcggggc gtcgctatcc gaacctggtc gacaacatgt gggcggcccg
     8221 ccacgtgccg cgcggcgatg cggtcgcgtg ttccggcgag gacgagtagg ggctagtccc
     8281 gcgccgcggc cgccaggcgg ttgatcccgt cgttttcgac caccgccggt gcatcggcca
     8341 tcgcggccag caccagcatc gcgcggccga ccgccgcggt gctggtcacg acgcccggca
     8401 gcaggcgcac gcccagtccc aacaatggag cgcccagcgc atacagcggg cgcatccagg
     8461 catgcgggct gcgctcgcca tgggcgggct ggatgccgcc ggggcgcagc atcacggtgg
     8521 tgatcggcag cgcccgcagg gcagcctcgg cctcgccctt gacccgcagc ggcatcaggc
     8581 ggctacccgg gttggcgtgc gcgccggaca cgtacaggaa ccgcccgccc ggattccgct
     8641 cggcgaacac gcgcgccacc tgcagggtca gcgccacggt gacgtggcgg tactcggctt
     8701 ccggcgtgcc cagcggcggc gcgcctgcgc agtagaaaca ggcgtcccac ggcgccagcc
     8761 ggccggccac gccgtccagc gcgtcgaagc gctcgacctg caggtcctcc acccgcgggt
     8821 cgtcgttgcc ggtgccgcgc ctgccgagca cgccgacccg cgtcacccgc ggatccgcga
     8881 gtacctcgcg cagcacgccc tgtccgacca gtccggtggc gcccgtcatc aggatgcgca
     8941 tggtggtgcc cgtggggtgg gaatcgctgc gagtgtaccc gcgcgctgcg ttgatgccgt
     9001 ggtgccgggg cgtgcacgga ttgatcgcgg tcaatgtcgc ccgcccgcgg cggtgccacc
     9061 ctgcccggac ccaatggagg aaacaccatg aaggcgaacg tgggcggcat cgacagatgg
     9121 atccggatcg tgctcgggtt ggcgttgatc gcgtgggcgg cgaccggtgg gccggcgtgg
     9181 gcgtggatag gtgcagtgcc gctggccacc ggcgtcttca atttctgcct gctgtatcgc
     9241 ttgctgggca tcaacacctg ccatcgttga cgcggcttcc accgggccac ggcaccgggg
     9301 cccggcggta acggcttcgc ctgcgcttca cgcaagcccc atccgccgct caccggcggc
     9361 gcggacactg ccgcctcacg caccagcgca gggacagggc aatgacaggt ggatcggcga
     9421 tcgaaaaggg accgcgcggg aaccggctgc ggccgttcgt ctggggcgcg gccgcatgcc
     9481 tgctgttgct gccggcggtg gcgatgcagt ggttcccggc gagcgaagtg aattggacgg
     9541 ccctcgactt catcgcgatg ggcgtgctgc tggccatcct gtgcggcctc tatgagctgg
     9601 gcgcgtggat gagcgacaac atcgcctacc gcgcgggctt cgggctggcg gcactgaccg
     9661 gcttcctgac catctgggtc aacctggcgg tggggatgct gggtgacgag gacggcacca
     9721 tcaacctggt gttcgccggg gtgctgttca tcgccaccgc cggcgccctg atcgccgggc
     9781 tcaggccgcg cgggatggcg ctggcgatgc tcgcggcggc gatcgcgcag ctggcggcgg
     9841 tgggcgtggc gctggcgatg ggtggatacg acgcacgcga gctcaccttc accgccatgt
     9901 tcgggttgcc gtggctggcg tcggcggcgc tgttccgcaa ggcggccgcg gacgcacggg
     9961 gactgccagg ggcctgctga gcgcaggctc gcgccgcggg cctcgattcg ccgggcgtgt
    10021 ttccgggaca atgggccgct cccgttcgag tccgcctacc cgacatgctc gcgctgcgcc
    10081 tggtcctgct gttcctgctg atcgccgcca atacgctcct gcacgtaccg ccgctgctgg
    10141 tggtggcgct gttcaaggcg gtgttgccgt tccagcgcgt gcgggtggcg agcaatccgg
    10201 tgctgaccgg attggcggaa agctggatcg gggtgaacag ctggatgtgg gatcgtttca
    10261 ccggcacccg cctgcagctg gatgaggacg tggcgctgca ggccgatggc cattacctgg
    10321 tgctggccaa ccaccagagc tgggtcgaca tcctggtatt gcagaaggtg ttcaaccggc
    10381 gcatcccgct gctgcgcttc ttcctgaagc gccagctgtt ctgggtaccg ttgctggggc
    10441 tggcgtggtg ggcgctggac ttcccgttca tgggtcgcta cacgccgaag caggtcgcgc
    10501 gccgccccga actggcccgg cgcgacatcg aggcgacccg ccgcgcctgc gggaaattca
    10561 gcgcgatccc ggtttcgatc atgaacttcg tcgaaggcac ccgcttcacc gtcgccaagc
    10621 atgcggcgca gggggcgccc taccagcacc tgctgaagcc gaaatcgggt ggcgtggcgt
    10681 tcgtgctgga tgcgatgggc gcgggcctgc acgcgatcct cgacgtgacc atcgtctacc
    10741 ctgccggccg cccatcgctg ctggacctgc tggcaaaccg catcccggcg gtacatgtgc
    10801 aggtgcgcca gcgcgagatc ccgccagcgc tgatccacgg ggactaccag ggcgaccgcg
    10861 ggttccgcgc cgggttccag caatggatca acggcatctg gcgggagaag gacgcggacg
    10921 tcgcgaccat gcttgcaggc agcgcccacc gggcctgaag gcgcgacccg cggcgcccgc
    10981 attgaaggcg gtgcgctgtt gcgcgcagcg gatcagcgcc gcggtttggc cggggccggg
    11041 ggtggcgagc cgtcgtcgcc gtcgatcttc ttcaacgaac gggtcacccc aagcacttcg
    11101 atcttgccgc cggccttgtg gaatgcatcg aggtcggcgg cgatgcgctc gtgggtcatg
    11161 ccgggattct tggcggcggg acgcggcgag aacaggtggc tggtcttgga gggcggcacc
    11221 tggggcgcgg cgggcgtcgc gggaggcggc gcaggcttct tcgggcgggg catgtggtcg
    11281 tcctttcagt aaagcgtgag cgggatgccg cgggcgttgc ggtcggcgta gtcggcgcga
    11341 acgtcgcggc agtacggtcc ccccatcggg aactttcggc agatcgtggg gcgcagttcg
    11401 tagatcgagc agcacatccg cgcctggtcg atcgccacgc accagccatc ctcgtcgcgg
    11461 gccatgactt cgacgccatg ctcgttgcgg gcggtgaagc gcagcggcac gtcgtcttcg
    11521 ggcatcagca ccacggtcag gcggcagcag acggcatcgc agcgatcgca gtgcggggct
    11581 tcggtgggcg tcgcggaggc cgtcacgcga cgatccgtcg ccggcaatgc agcaggcacc
    11641 cgcaaggccg gacccggccg gacccgctcc gatgcgccag aaggatgccg aggcgcgcac
    11701 gccgtccatc ggggtcggcg aaccgccggg tgtcgttgcg caagcgcagg cgatcgcgat
    11761 gtccgtcgat gggatgctcg gtctcgcgga tcgggtaacc cggcatgggc gctccggagg
    11821 ggcgtcgccc ccgggcaggg gtggtgacgc gtggggggag gcgcagcgag cgacgatgga
    11881 ggcgggtcgg acggacgcac gtggcaacgc agtgccgccc catcctacgc gataaggccg
    11941 ggccgcgccc cgccggcgac gtgccgcgca ggcgcgcgcg caaggcgcat actcgggcgc
    12001 caggtggagc accgcatgtc ccagcaccag atcgccctgt tcacgttcac gcttgggaat
    12061 accgaaagca tcagcccggt ggcactgcgc gacctgtggg tgcgtgcggc cggcatcggc
    12121 aatgtcagcg tgggccgcaa gtcgccgcac ggcagctacc gcgaccgccc gacctacatg
    12181 ctgtacgcac cgcaaagcct gggcaacctg cgcgacgtgg agctgcgcct gcgcaagctg
    12241 ctcgaggatg cgcacctgaa cgcatcgctg acgccgctgc acgcctgagc cgcggcaacc
    12301 gggcttgcgc ggtggcgatg gcggctgcgc gggccgggtt cggggccggt acggccggcc
    12361 gccgggatca gaagcggtcg cccaccggca ggtagcgcca ctggcccacc ggcatcttcg
    12421 ccagcgatac cttgccgatg cgcaggcggc ggatcgccac cacgtccagc cccacctgct
    12481 tgcacatgtc gcgaagttgc ccgccctgca cgcccttcag ggcgaagcgc aggcggactt
    12541 cgttctgcca gctgaccttg cacggcggca gcgggcggcc gttgtagtgc aggccatggt
    12601 tgagcttgcg cagtccgtaa ggggcgatct cgccactgac ctcgaccacg aattcctgtt
    12661 cgatcaggtc ctggtcctcg gtcaggcgcc gccagatgcg gccgtcctgg gtcagcacca
    12721 tcaggccact ggcgtcgcga tccagcggta ccagcggggt cagccgctgg aaatggcgct
    12781 ccagcaggcg cacacccgag gggtcctcgg tccagtgcgt ctccggcgcc accagcgccg
    12841 tggccgggtt ggcgccgctg atggtgtcga agccggccgg cttgtgcagc agcagggtgg
    12901 ccggttccac cgcttccagc agcgcgtccg cgtcgatgct gactgcttca tcggtcacca
    12961 gcaggtgcgg gatttccacc acgcgcccat ccacgcgcac ccagccgcac ttgatgtatt
    13021 gctcggcggc gatccgcgag caacgcgcga gttcggccac gcgcttggcc agtcggaccg
    13081 gttcggacat cgggcagggc ctcgaaaggg gacatgcagt gtatagatca ggcactctgt
    13141 cgccccggat ttgcgccgtg cgcccgcgac gcgcgcgcgg cggccgggcc tccccacctt
    13201 ccgagatgcc atgaaaaccc cttcgggcct gcagccgctg atcgacgacg gcgtcatcga
    13261 cgaggtcatg cgtccgctga aaagcggcaa ggaagccgcc gtctacgtgg tccgcgccgg
    13321 caacgaggtg cgttgcgcga aggtctacaa ggacatggcg cagcgcagct tccagcagcg
    13381 cgtgcagtac caggaagggc gcaaggtgcg cggcagccgc gaggcgcgcg cgatcggcaa
    13441 ggccagcaag tacggccgca agcagcagga ggccgcctgg aagaacaccg aggtggatgc
    13501 gctgtaccag ttgcgcgacg ccggggtgcg ggtgccggaa ccgttcggtt acttccacgg
    13561 cgtgctggtg atggaactgg tggtcgatgc cgacggccat tccgcgccgc ggctgggcga
    13621 ggtcgaactg tcgccggagc aggcgcgcgg cttccaccgg ttcctggtgc ggcaggtgat
    13681 caggatgctg tgcgtgggcc tgatccacgg cgacctgtcc gaatacaacg tgctggtcgc
    13741 gccggatggc ccggtgatca tcgatttccc gcaggtggtg agcgccgccg gcaacaacgc
    13801 cgcgcgcacg atgctgctgc gcgacgtcaa caacctcacc gccagcctcg gcggatgggc
    13861 cccggaactg ctggaaacct ggtacggcga ggagatgtgg gcgctgttcg aggccggcga
    13921 gttgcatccg gacagcgaac tcaccggcgc gttcgtgcat gacgaatcca cgccggacat
    13981 cgacggcgtg cggcatgcga tcaacgatgc gcgcgaggaa gcactgatcc gccagcaggg
    14041 acgcgaagca gcggcgcagg acgattgagc ctgcgatcga cggtcgcggt cagcgccgcg
    14101 ttgccaatac gccgtccagc gccggcccgg ccctgaagcc cgacttttcc agccgcctgg
    14161 cgacctcgcg cggcaggtcg cgtccgctgg tgagcctgtt gggggtgccg gtgtagtgga
    14221 acaggcggcc accgcgcttg atcacccgcg caagctggtc gtagaaggcc tgcgaataca
    14281 gttcgccggc gatgccgaag cgtggcgggt cgtgcagcac cgcatcgaag ccggcatcgg
    14341 gcaaggccac gatcgccttc gaaacgtcgg cgtgcgccag ttgcaggcga ccgccgctgg
    14401 ttgcgggatc cggcgaccac gggttcagcg cgcgcagcca cagcacgtcc tcgttcttct
    14461 cgaacgactg gatcgatgcc accccggcat ccaggcaaca cgcggcgaag tagcccagac
    14521 cgccgcaggt gtccagcacg cgcatgccgg cgggctgcac cagcgcgacc ttgcggcgcg
    14581 catcgtcgac cggtgattcc ttcgaggtcg gcagcatctt gatgccgtcg atctcgaagg
    14641 tcggggcatc ccattcggtc ggcaccagct tgatcagcga acccgcgtag cgcgaaacgg
    14701 ccgcgaagcc gtcgccatcc caccagtaga tggtgcgctc cttcagcgtg cccggatacg
    14761 gatagtgctg gccgcgccat tcccaaccat cggcgcgcag tgcggccgct gcgctgctgc
    14821 gcccgaggtc gagcgagccc gaccacgcgg gttcgccggc atcgcgcgcg
//
LOCUS       DAORUK010000045        14739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_3888_length_14739_cov_18.574775, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000045 DAORUK010000000
VERSION     DAORUK010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 14739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14739
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3888_length_14739_cov_18.574775"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PK818_04650"
     CDS             complement(<1..563)
                     /locus_tag="PK818_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005912482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HQY82060.1"
                     /translation="MTKKAIFDFLERERAMPARIPLQLRRDGDWSELYGRFGEDEAKH
                     QAGRCLDCGNPYCSWGCPLHNRIPQWLELAREGRIIEAAALCHETNPLPEICGRVCPQ
                     DRLCEGSCTLNDGFGAVTIGAVEKYIVDAALAQGWTPDLSQAPATGKRVAIVGAGPAG
                     LACADRLARAGIAATVFDRYEKIGGLL"
     gene            complement(560..5062)
                     /gene="gltB"
                     /locus_tag="PK818_04655"
     CDS             complement(560..5062)
                     /gene="gltB"
                     /locus_tag="PK818_04655"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HQY82061.1"
                     /translation="MTECDQPSPEPMGLYDPGDDRDSCGFGLIAQLDDRASRANVDAA
                     LDALARMTHRGGVAADGLSGDGCGLLLKQPERFLRTLAKEAGFALGSQFAAGLVFLPH
                     DDAEAARCRETLRTALAEVRLAVAGWRQLPVDADACGATARANMPRIEQVFAAPLIDV
                     EGERPTPEAFRRALYLARRRATQRLQDVPAFYIVSLSADSIGYKGMVLPARLPQLYPD
                     LRRTDLESSVVVFHQRFSTNTSSRWSLAQPFRMLAHNGEINTIAGNRAWAQARAQVWR
                     SEQLDPREFDPVIEVDGSDSQSLDAMLDLLEAGGMDALKAMRILVPPATQSLEYKDAD
                     LAAFYEYYALNSEPWDGPAGIVLFDGRYAACTLDRNGLRPARWQLTQDRHFLIASEAG
                     VWDVPSAQVVRKGKLGPGQMIAADLRDGSLLESDAIDAINRARAPYKRWLRQGMTYLH
                     SELIDPALADAPFDHATLAGFQKLFQLTREEREKVLRPLAEMEQEATGSMGDDVPMAT
                     LSQQVRPLYDRFRQAFAQVTNPPIDPLREDCVMSLATQLGREGNVFADSAETVRHVHL
                     GSPVLSQRKLRQLLAMPPYDSAYRYIELGFDAATGMRAAIEAVCAQAEAAAREGVLLL
                     ILSDRRPRREQLMLHALLATGAVHQHLLRRGLRCAVNLIVETGTARDPHHFACLIGYG
                     ATAVYPYLAYQTLHDLGVRGILKTKHGGHASEEPAQIGRSYRRGIRKGLLKIISKMGI
                     STIGSYRGARLFEIIGLDREVTELCFAGTPSRIGGAGFAELQAEAEHLARIGWDNNAL
                     PEIGGLLQFTPDGEYHQFNPEVVQALQRAVMTGTPEDWARYADAVNSRPPAALRDLLA
                     LDTTTSNAIPLAEVEPVDAIVRRFDTAAMSLGALSPEAHEALAIAMNRLGGRSNSGEG
                     GEDPARYRSDKVSKIKQIASGRFGVTPEYLVNAEVLQIKMAQGAKPGEGGQLPGHKVN
                     AMIARLRYARPGIGLISPPPHHDIYSIEDLAQLIFDLKQVNPDALVSVKLVSHAGVGT
                     IATGVAKAGADLITISGHDGGTGASPISSIRYAGTPWELGLSEARQGLLANGLRERVI
                     VQADGGLKTGLDVIKAALLGAESFGFGTAPMIALGCKYLRICHLNNCATGIATQDDRL
                     RADHFTGLPERVENFFRGLAEEVRGYLAQLGVRTLGELVGRTDLLRQLDTLAATGERI
                     DLSLLLAGDGLAHGGHCGTPRLAASTDGLAAELDADLDLVLTHALGGSFAYPIRNTDR
                     SIGARLSGRIARLHGDHGMREAPIDLHFTGSAGQSFGAFLASGLRLTLEGEANDYVGK
                     GMAGGRIVIRPPRDAQYVACDTPILGNTCLYGATGGELYAAGHAGERFAVRNSGAIAV
                     VEGVGDHCCEYMTGGVVAVLGRTGLNFGAGFTGGVAYVLDLDRDFVDRYNHELIDILR
                     INNEGFEAHRSHLTDLLETHVAQTGSVFAQRILDDFRDFLGKFWLVKPKAASLASLVE
                     TLRRAA"
     gene            5192..5944
                     /locus_tag="PK818_04660"
     CDS             5192..5944
                     /locus_tag="PK818_04660"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQY82062.1"
                     /translation="MRILLLALSLLLSACANAPQRNPLAEWVPSPNFEARRPVIIVVH
                     ATEQGSARQSLETLRTANSKGPVSSHYLVGDDGRIYQLVADGDRAWHAGGGSWGTITD
                     LNSASIGIEIDNQVGEPYTEAQVAALLRLLDDLCTRLGIPRTQVIGHADMAPTRKRDP
                     GSLFPWQRLADAGFGRWPQGELADPPPGFDPWLAMAVVGYPLKDRAAAVRAFHRHYRG
                     RDDGEDPDAGFDGEDLRILHALAAAVVAPPAR"
     gene            5980..6732
                     /locus_tag="PK818_04665"
     CDS             5980..6732
                     /locus_tag="PK818_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQY82063.1"
                     /translation="MPRAPVGFMIACAARTGSTMLVRTLRSHPGLVVHGEVWGDGMVG
                     LDGPLARGCEADPAAFAALEAARFDAPADTLARFLDPHGAQAVGFKLKFDELVRPQWR
                     GIRRLVEADVGLRIVFLHRRDLLRRYLSHQVVLRQTGVTNVPAGGDVPGIEPFEVDIQ
                     DCLRDIAETRRRSREFEAAFAAHPSLQVVYEDLAADPQAACERVFGFLGVAPVPVRVA
                     TERIVRMPPEALVRNYEALRAALDAAGEAVAA"
     gene            complement(6778..7719)
                     /locus_tag="PK818_04670"
     CDS             complement(6778..7719)
                     /locus_tag="PK818_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQY82064.1"
                     /translation="MAPLAAAAQDIPATADPAIPRTPVAAPASTIDGGATPELRAQVL
                     LERAWFSPGELDGKWGRNSRGALADFQHARGLPPSGEPDAATWAALALDPAPVLVDYT
                     IGEGDVAGPFLPTPAGFMAKSLMKSIPYQSAAEALGEKFHASPALLAALNPGIPLDVA
                     GSVIRVPNVLDLPPLPPAALVQVDRSDAALRLLDADGRAYARFPITSGTAKFPLPIGD
                     WTIRSINPDPWYNYDPRLIVSARRGDRKALLPPGPNGPVGSMWMGLSKPHYGIHGTPE
                     PGLIGRTQSSGCVRLANWSAHAVAGAVYAGITVRMVE"
     gene            7956..9008
                     /locus_tag="PK818_04675"
     CDS             7956..9008
                     /locus_tag="PK818_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY82065.1"
                     /translation="MTADLLPDPAIDALATPVLRLDHAGVVVQANAAAGAWLEVGRRR
                     LLGLHAVALEREGDGLAAALSQALEAPTRLRRVALACPGSDALRFADLWLVADAHGHW
                     LEAHPVDEFPGEDPARLLPAALSASLKGLAHELRNPLAGIKGAAQLLARRVDDDGREL
                     TALIETEVERLAALVDRLLSPSPPRAFEPLNIHVVLERVLRLAESDAGWAVRLQRDYD
                     PSLPELAGDADRLTQAVWNLVRNAIEAGAGSVQLRTRAEHGVRIGEDVHALALRLEIA
                     DDGHGVPADLAERVFLPLVSGRADGSGLGLALAQQVAREHRGSLTFRSRPGHTVFTLL
                     LPLPSESQDASGTTDG"
     gene            9001..10434
                     /gene="ntrC"
                     /locus_tag="PK818_04680"
     CDS             9001..10434
                     /gene="ntrC"
                     /locus_tag="PK818_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HQY82066.1"
                     /translation="MAEPERIWVVDDDRAVRFVLATALRDAGFEVTAFAAAAEALDTL
                     QRDIQARRGAPALVVTDVRMPGDSGLQLLEKLKAAKPDLPVIVMSAHTDVASTAGAFR
                     GGAHEFLSKPFDLDEAVALVRRALPQRDARGPRDAQLPVDAGDADAATLVGDAPAMRA
                     LFRAIGRLAQAPLSVLITGETGTGKELVARALHRESPRASRPFVALNTAAIPAELLES
                     ELFGHEAGAFTGAHKRHIGRFEQADGGTLFLDEIGDMPAPLQTRLLRVLAEGEFFRVG
                     GRELIRVDVRVIAATHQPLQALVEAGRFRADLLHRLDVVRLPLPPLRERREDIPQLAA
                     RFLANAARRLQLAPKKLSAAALERLRGHDWPGNVRELENLCWRLAALAPGASIGVADL
                     DEAMPAARAVADAGGHWQQALAAWARAQLDAGVHDLHAAARREFDRVLLQAALEHSEG
                     HRGEAAHRLGLGRNTVTRKLGPSRTRR"
     gene            10508..11071
                     /locus_tag="PK818_04685"
     CDS             10508..11071
                     /locus_tag="PK818_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017165149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HQY82067.1"
                     /translation="MTHATPTRLVATIACSLLAACASTTPAPAPKPATGTAQEAVVVL
                     APASGSLVSGKLVLRPQGDGVHIRGEVGGLKAGDVRGFHIHERGDCSAADASSAGGHF
                     NPAGSAHGRAGQGAHHAGDTDNIVADARGVARIDAHVRGVTLGGGAANDIAGRALIVH
                     AAADDYSTQPTGNAGGRVACGVVRVLR"
     gene            complement(11072..11938)
                     /locus_tag="PK818_04690"
     CDS             complement(11072..11938)
                     /locus_tag="PK818_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82068.1"
                     /translation="MDDTGQQVFRGVVALELRDGAPPGRAALDAGDAGAATALLGRDL
                     AALVPGVRDCDLALLAAHFDPAEALRPGWPLHRRALELLQRAPGQAQGARMVGFGADA
                     RGDVPVPLQCDPNLAGGGLRVLPFVLRGAPAVAAGAELEGLLLDRGMAAADTALALQD
                     GLAAQVEHARYLSLHDLLAMIALQYRNVGLEPLWSLLETALLEPAAESWLDAGPEPLA
                     HFADGEVRIALFDPGAWHARYAPGAADCSALERGFEYFQARQRQFAAVLEAHAVPVQF
                     VHCADGNADCLR"
     gene            12025..13302
                     /locus_tag="PK818_04695"
     CDS             12025..13302
                     /locus_tag="PK818_04695"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HQY82069.1"
                     /translation="MLHGRPVAILGGVRIPFCRQNTAYADVGNLGMSVRTLGALVEKF
                     GLHGQQLGEVAMGAVIKHSSDWNLGREAALSSGLSALTPGITLQRACGTSLDTIIHIA
                     GKIATGQIESGIGGGSDTTSDVPIVYGQSLRRRLLKAAAAKTTGDKLAAFKGFKLSEL
                     KPDFPGVAEPRTGKSMGNHCEDMAKEWNISRDSQDELAVASHHKLAAAYERGFFDDLV
                     VSFRGVSRDNVLRPDSSLEKLSTLKPAFDKSSGRGTLTAGNSTPLTDGASACLLASDE
                     WAAAHGHEVLCHLRDAQVAAVDFVHGEGLLMAPTVAVAEMLKRNGLTLQDFDFYEIHE
                     AFAAQVLCTLRAWESEDYCRTRLGLEAPLGRIDPAKINPNGSSLAAGHPFAATGARIV
                     AAAAKELKLRGGGRCLVSICTAGGMGVVAILER"
     gene            13336..14124
                     /locus_tag="PK818_04700"
     CDS             13336..14124
                     /locus_tag="PK818_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82070.1"
                     /translation="MRDIDDGMVPPAARRGKQRHRARLIGLLALLVAVPSVTVGSDRE
                     PALRMLFVGNSYLYVNDLPSQVRRMAASRGVALDIRMLAQPDFALSDHLRGPDFLREL
                     DRDWDWIVLQQGPSSLPESREDLIASVRVVAARVQGKPVRVALMSGWPAKQYAAHSSQ
                     GEVSYRLAATHIGACVMPVATAWRLARESDKSTTLYAPDRLHATRAGTRLAAMVVLHG
                     LLGEGPQPATQDRSDTPAKPKRVDVLEQAVIRAYEDEPKGCGPD"
     gene            complement(14165..>14739)
                     /locus_tag="PK818_04705"
     CDS             complement(14165..>14739)
                     /locus_tag="PK818_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82071.1"
                     /translation="GRVVTNGMSQYSRNERNANAGIVVGITPEDYTPHGEGPLAGIAL
                     QRHWESQAYALGGGDYLAPAQRVGDFIRNRPSTRFGAVLPSYTPGVTLGSLDEALPRY
                     AIDAIREALPAFERQIRGFAMEDALLTGVETRTSSPLRITRGEDGHSRNTRGLFPAGE
                     GAGYAGGILSAGVDGIKTAEAVARSILRRT"
BASE COUNT         2092 a         5117 c         5350 g         2180 t
ORIGIN      
        1 tgcagcaggc cgccgatctt ctcgtagcgg tcgaacacgg tggccgcgat gccggcgcgc
       61 gcgaggcgat ccgcgcaggc gagtcccgcc ggtcccgcgc cgacgatggc cacgcgcttg
      121 ccggttgccg gtgcctgcga aagatccggc gtccagccct gcgccagcgc ggcatcgacg
      181 atgtatttct ccaccgcgcc gatggtcacc gcgccgaagc cgtcgttgag cgtgcagctg
      241 ccttcgcaca ggcggtcctg cgggcacacc cggccgcaga tttccggcag cggattggtt
      301 tcgtggcaca gcgccgccgc ttcgatgatg cggccttcgc gcgccagttc cagccattgc
      361 gggatgcggt tgtgcagcgg gcagccccag ctgcagtacg ggttgccgca gtccaggcaa
      421 cgcccggcct ggtgcttcgc ctcgtcctcg ccgaagcggc cgtagagctc gctccagtcg
      481 ccgtcgcggc gcagctgcaa cgggatgcgc gcgggcatcg cgcgttcgcg ctcgaggaaa
      541 tcgaagatcg ccttcttcgt catgcggccc tccgcaatgt ctcgaccagc gaggccaggc
      601 tcgccgcctt cggcttgacc agccagaact tgccgaggaa gtcgcggaag tcgtcgagga
      661 tgcgctgcgc gaacacgctg ccggtctgcg cgacatgggt ttccagcagg tcggtgaggt
      721 gcgagcggtg cgcctcgaag ccttcgttgt tgatccgcag gatgtcgatc agctcgtggt
      781 tgtagcggtc gacgaaatcg cggtcgaggt cgagcacgta ggccacgccg ccggtgaagc
      841 cggcgccgaa gttcaggccg gtgcggccga gcaccgcgac cacgccgccg gtcatgtatt
      901 cgcagcagtg gtcgcccacg ccttcgacca ccgcgatggc gccggaattg cgcaccgcga
      961 agcgttcgcc ggcgtgcccg gccgcataca gttcgccgcc ggtggcgccg tacaggcagg
     1021 tgttgccgag gatcggggtg tcgcaggcga cgtactgcgc atcgcgcggc gggcggatca
     1081 cgatgcggcc gccggccatg cccttgccga cgtagtcgtt ggcctcgccc tcgagggtga
     1141 gccgcaggcc gctggcgagg aacgcgccga agctctgccc ggcgctgccg gtgaagtgca
     1201 ggtcgatcgg cgcctcgcgc atgccgtggt cgccgtgcag gcgggcgatg cggccggaca
     1261 ggcgcgcacc gatgctgcgg tcggtgttgc ggatcgggta cgcaaagctg ccgccgagcg
     1321 catgcgtcaa cacgaggtcg aggtcggcat ccagttccgc cgccaggcca tcggtggatg
     1381 ccgccagccg cggcgtgccg caatggccac cgtgcgcgag gccatcgcct gccagcaaca
     1441 gcgacaggtc gatgcgctcg ccggtcgcgg ccagggtgtc cagctgccgg agcaggtcgg
     1501 tgcggccgac cagttcgccc agcgtgcgca cgccgagttg cgccaggtag ccgcgcactt
     1561 cttccgccag tccgcggaag aaattctcca cgcgctcggg caggccggtg aagtggtcgg
     1621 cacgcaggcg gtcgtcctgg gtggcgatgc cggtggcgca gttgttgagg tggcagatgc
     1681 gcaggtactt gcagccaagc gcgatcatcg gcgcggtgcc gaagccgaag ctctccgcgc
     1741 cgagcagtgc cgccttgatc acgtcgaggc cggtcttcaa gccaccatcg gcctgcacga
     1801 tcacccgctc gcgcaggccg ttcgccagca ggccctggcg cgcttcggac aggcccagtt
     1861 cccacggcgt gccggcatag cggatcgatg aaatcggact cgcaccggtg ccgccgtcgt
     1921 ggccggagat cgtgatcagg tcggcgccgg ccttcgccac gccggtggcg atggtgccga
     1981 cgcccgcgtg cgacaccagc ttcaccgaaa ccagcgcatc cggattcacc tgcttcaggt
     2041 cgaagatcaa ttgcgcgagg tcttcgatcg aatagatgtc gtggtgcggc ggcggcgaga
     2101 tcaggccgat ccccgggcgc gcgtaacgca gcctggcgat catcgcgttg accttgtggc
     2161 cgggcagctg gccgccctcg ccgggcttgg ccccctgcgc catcttgatc tgcaggactt
     2221 cggcgttgac caggtattcc ggggtgaccc cgaaacggcc cgacgcgatc tgcttgatct
     2281 tcgagacctt gtcgctgcgg tagcgcgccg ggtcctcgcc gccctcgccg gaattgctgc
     2341 gcccgccgag ccggttcatc gcgatcgcca gcgcttcgtg cgcttccggc gacagcgcgc
     2401 cgagcgacat cgccgcggtg tcgaagcggc gcacgatggc gtcgaccggc tcgacctcag
     2461 caagcgggat cgcgttcgac gtcgtggtgt cgagcgccag caggtcgcgc aacgccgccg
     2521 gcggccgtga gttcaccgca tcggcatagc gcgcccagtc ttcgggcgtg ccggtcatga
     2581 cggcgcgctg cagcgcctgc accacctccg ggttgaactg gtggtattcg ccgtcggggg
     2641 tgaactgcag caggccgccg atttccggca gcgcgttgtt gtcccagccg atgcgggcga
     2701 ggtgttcggc ttcggcctgc aattcggcga agccggcgcc accgatccgc gatggggtgc
     2761 cggcgaagca cagctcggtg acctcgcggt cgaggccgat gatctcgaac aggcgcgcgc
     2821 cgcgatagct gccgatggtg ctgatgccca tcttcgagat gatcttgagc aagcccttgc
     2881 ggatcccgcg ccggtagctg cggccgatct gcgccggctc ctcagaggca tgtccgccgt
     2941 gcttggtctt gaggatgccg cgcacgccga ggtcgtgcag ggtctggtag gccaggtacg
     3001 ggtacaccgc ggtggcaccg tagccgatca ggcaggcgaa gtggtgcgga tcgcgcgcgg
     3061 tgccggtctc cacgatgagg ttgaccgcgc agcgcaggcc gcggcgcagc aggtgctggt
     3121 ggaccgcgcc ggtggcgagc agggcatgca gcatcagctg ctcgcggcgc gggcggcggt
     3181 cgctgaggat cagcagcagc acgccttcgc gcgcggccgc ctccgcctgc gcgcagaccg
     3241 cctcgatcgc cgcgcgcatg ccggtggcgg catcgaagcc gagttcgatg tagcggtagg
     3301 cgctgtcgta cggtggcatc gccagcagct ggcgcagctt gcgctggctc agcaccggcg
     3361 aaccgaggtg gacgtggcgc acggtctcgg cgctgtcggc gaacacgttg ccctcgcggc
     3421 cgagttgggt ggccagcgac atcacgcagt cctcgcgcag cgggtcgatc ggcgggttgg
     3481 tgacctgcgc gaacgcttgc cggaaacggt cgtacagcgg ccgcacctgt tgcgagagcg
     3541 tggccatcgg cacgtcgtcg cccatcgagc cggtcgcctc ctgttccatc tccgccagcg
     3601 ggcgcagcac tttctcgcgc tcctcgcggg tgagctggaa cagcttctgg aagccggcca
     3661 gggtggcgtg gtcgaacggt gcatcggcga gcgcaggatc gatcagctcg ctgtgcaggt
     3721 aggtcatgcc ctgccgcagc cagcgcttgt aaggcgcgcg cgcgcggttg atggcgtcga
     3781 tcgcgtcgct ttcgagcagg ctgccgtcgc gcaggtcggc ggcgatcatc tggccggggc
     3841 cgagcttgcc cttgcgcacg acctgcgcac tcggcacgtc ccacacgccg gcttccgacg
     3901 cgatcaggaa atgacgatcc tgggtgagtt gccagcgcgc cgggcgcagc ccgttgcggt
     3961 ccagcgtgca cgcggcgtaa cggccgtcga acaggacaat gccggccggc ccgtcccagg
     4021 gctcgctgtt gagcgcgtag tactcgtaga acgcggcgag gtcggcgtcc ttgtattcca
     4081 gcgactgggt ggccggcggg accaggatgc gcatcgcctt cagcgcgtcc atgccgccgg
     4141 cttccagcag gtccagcatc gcgtccaggc tttgcgaatc ggagccgtcg acctcgatca
     4201 ccgggtcgaa ttcgcgcggg tcgagctgct cgctgcgcca cacctgcgcg cgcgcctgcg
     4261 cccacgcgcg gttgccggcg atggtgttga tctcgccgtt gtgggcgagc atgcggaacg
     4321 gctgcgccag cgaccagcgc gagctggtat tggtggagaa gcgctggtgg aacaccacca
     4381 cgctgctttc caggtccgtg cggcgcaggt ccgggtacag ctgcggcagc cgcgccggca
     4441 gcaccatgcc cttgtagccg atgctgtccg ccgacaggct gacgatgtag aacgccggca
     4501 cgtcctgcag gcgctgggtg gcgcggcgcc gcgccaggta cagcgcgcgc cggaacgcct
     4561 cgggtgtcgg ccgttcgccc tcgacatcga tcagcggggc ggcgaacacc tgctcgatgc
     4621 gcggcatgtt ggcgcgcgca gtggcgccgc aggcgtcggc atccaccggc agctggcgcc
     4681 agccggcgac cgccagcctc acctcggcca gcgccgtgcg cagggtctcg cggcaacgcg
     4741 ccgcctcggc gtcgtcgtgc ggcaggaaca ccagcccggc ggcgaactgc gagcccagcg
     4801 cgaagccggc ttccttcgcc agcgtgcgca ggaagcgttc cggctgcttg agcagcagcc
     4861 cgcagccatc gccgctgagg ccgtccgcgg ccaccccgcc gcggtgggtc atgcgcgcca
     4921 gcgcgtcgag cgcggcatcg acattggcgc ggctggcgcg atcgtcgagt tgcgcgatca
     4981 ggccgaagcc gcagctgtcg cggtcgtcgc cggggtcgta caggcccatg ggctcagggc
     5041 tgggttggtc gcactccgtc atggcggttc caaggctcgc tgcactgcaa catgacccga
     5101 gcctagaaca aaaaaccagg gccgtgctcg gtagttgcga cggaaaccaa tcgaggacgg
     5161 aaaccggcgc cgccagcgtt aagatcggcg catgcggatc ctcctgctgg ccttgtcgtt
     5221 gctgctgtcc gcctgcgcca atgccccgca gcgcaatccg ctggcggaat gggtgccatc
     5281 gcccaatttc gaggcgcggc ggccggtgat catcgtggtc cacgccaccg agcagggctc
     5341 cgcccggcag agcctggaga ccctgcgcac ggccaacagc aagggaccgg tcagttcgca
     5401 ctacctggtc ggcgacgacg gccgcatcta ccagctggtc gccgatggcg accgcgcctg
     5461 gcatgccggc ggcggctcgt ggggcacgat caccgacctc aattcggcct cgatcgggat
     5521 cgagatcgac aaccaggtcg gcgagcccta caccgaggcc caggtcgcgg cgctgctgcg
     5581 cctgctggac gacctgtgca cccggctcgg gatcccgagg acgcaggtca tcggccatgc
     5641 cgacatggcg ccgacccgca agcgcgaccc cggttcgctg ttcccgtggc agcggctggc
     5701 cgacgccggc ttcggccgct ggccgcaggg cgagctggca gacccgccac ccggcttcga
     5761 tccgtggctg gcgatggcgg tggtcggcta tccgctgaag gatcgcgccg ccgcggtacg
     5821 cgcgttccat cgccactacc gcggccgcga cgacggcgag gacccggatg cgggtttcga
     5881 cggcgaagac ctgcgcatcc tgcatgcgct ggccgccgcg gtggtcgcgc caccggcgcg
     5941 atgaccctat ccttgccggc cgttccaggg ccgatgccga tgccgcgagc tcccgtgggt
     6001 ttcatgatcg cctgcgccgc gcgcaccggc agcacgatgc tggtgcgcac cctgcgcagc
     6061 catcccggcc tggtcgtgca cggcgaggtg tggggcgatg gcatggtcgg gctggacggc
     6121 ccgctggcgc gcggctgcga ggccgatccc gcggcattcg cagcactgga ggcggcgcgt
     6181 ttcgacgcac ccgccgacac cctggcgcgt ttccttgacc cgcacggcgc acaggcggtc
     6241 ggcttcaagc tgaagttcga cgagctggtg cgcccgcagt ggcgcggcat ccgccggctg
     6301 gtcgaagcgg acgtgggcct gcgcatcgtg ttcctgcatc gccgcgacct gctgcggcgc
     6361 tacctctcgc accaggtcgt gctgcgccag accggcgtca ccaacgtgcc ggcaggtggc
     6421 gatgtgccgg gcatcgagcc attcgaagtc gatatccagg actgcctgcg cgacatcgcc
     6481 gagacccgcc ggcgcagccg tgaattcgag gccgcgttcg ccgcgcatcc gtcgctgcag
     6541 gttgtttacg aggacctggc cgcggatccg caggccgcgt gcgagcgggt gttcggcttc
     6601 cttggcgtgg cgccggtgcc ggtgcgggtg gcgaccgaac ggatcgtgcg gatgccgccg
     6661 gaggcgctgg tgcgcaacta cgaggcgctg cgggcggcac tggatgcagc cggcgaagcc
     6721 gtcgcggcct gagcctcctg cggatcgcca ccgcacggcc acgagcgcgg gatccgccta
     6781 ctccaccatg cgcacggtga tccccgcgta gaccgcgccg gccaccgcgt gcgcgctcca
     6841 gttggccagc cgcacgcagc cgctggactg ggtgcggccg atcaggcccg gctccggcgt
     6901 gccgtggatg ccgtaatgcg gcttcgacaa ccccatccac atgctgccga ccggaccgtt
     6961 cgggcccggc ggcagcagcg ccttgcggtc gccgcgacgg gcactgacga tcagcctcgg
     7021 gtcgtagttg taccaggggt ccgggttgat cgaacggatc gtccagtcgc cgatcggcag
     7081 cgggaatttc gcggtaccgc tggtgatcgg gaagcgcgcg taggcgcggc cgtcggcgtc
     7141 cagcaggcgc agcgccgcat ccgaacggtc gacctggacc agggccgcgg gcggcagcgg
     7201 cggcaggtcc agcacgttgg ggacgcggat cacgctgccg gcgacatcca gcgggatgcc
     7261 ggggttcagc gcggccagca gcgccgggct ggcatggaat ttctcgccca gcgcctcggc
     7321 ggcggactgg taggggatgg acttcatcag cgacttcgcc atgaagcccg cgggcgtggg
     7381 caggaacggc ccggcgacgt cgccttcgcc gatggtgtaa tcgaccagca ccggcgcggg
     7441 atcaagcgcc agcgccgccc aggtggcggc atccggttcg ccgctcggcg gcaggccgcg
     7501 cgcgtgctgg aaatcggcga gcgcgccgcg gctgttgcgg ccccatttgc catccagttc
     7561 gccgggcgag aaccaggccc gctccagcag gacctgcgcg cgcagctcgg gcgtggcgcc
     7621 gccatcgatg gtgctggcgg gcgccgccac cggggttcgc gggatcgccg gatccgccgt
     7681 cgccgggatg tcttgcgcgg cggccgccag cggtgccagc agcaggacca agaacaggga
     7741 tcgcgggaag gcacgcatcg gtcggctcgg tggttggcgt attcagcttg catgaacatg
     7801 aacggaggcg gcgtgcacgc ccggcaaccg ttttgccggc gtttacccgc ggacgcttgc
     7861 cgggcgccgg cccttgcccc ggtttccgac gcattgggtg cttggccccg caagcgggcg
     7921 gtaccggcgc cggcaaattg cactaaatta gtgcaatgac cgccgatctc ctccccgacc
     7981 ccgcgatcga cgcgctcgcc accccggtgc tgcggctcga ccacgccggc gtggtggtgc
     8041 aggccaatgc cgccgccggt gcctggctgg aggtcgggcg gcggcgcctg ctcgggttgc
     8101 acgcggtggc gctggaacgc gagggcgatg gccttgcggc ggcgctgtcg caggcgctgg
     8161 aggcgccgac ccggctgcgg cgggttgcgc tggcgtgccc gggcagcgat gccctgcgct
     8221 tcgccgacct gtggctggtg gcggacgcgc acggacactg gctggaggcg cacccggtcg
     8281 acgagttccc gggcgaggat ccggcgcggc tgctgccggc agcgctgagc gcgtcgctga
     8341 agggcctggc gcacgaactg cgcaatccgc tggcggggat caagggtgcg gcgcaactgc
     8401 tggccaggcg ggtggacgac gacggtcgcg aactcaccgc gctgatcgag accgaggtcg
     8461 aacggctggc cgcgctggtc gaccggctgt tgtcgccgtc gccgccgcgc gcgttcgagc
     8521 cgctcaacat ccatgtggtg ctggaacgcg tgctgcggct ggcggagagc gacgccggct
     8581 gggccgtgcg cctgcagcgc gattacgacc cgtcgctgcc ggaactggcg ggcgatgccg
     8641 accgcctgac ccaggcggtc tggaacctgg tgcgcaacgc gatcgaggcc ggcgccggca
     8701 gcgtgcagct gcgcacccgc gccgaacacg gcgtgcgcat cggcgaggac gtgcacgcgc
     8761 tggcgctgcg gctggagatc gccgacgacg gccacggggt gccggcggac ctggcggagc
     8821 gggtgttcct gccgctggtc agcgggcgcg ccgatggcag cgggctgggg ctggcgctgg
     8881 cgcagcaggt ggcgcgcgag catcgcggct cgctgacctt ccgctcgcgc ccggggcata
     8941 ccgtgttcac cctgttgctg ccgcttccct ctgaatcgca ggacgcatcc gggaccaccg
     9001 atggctgagc cggaacggat ctgggtggtg gacgacgacc gcgcggtgcg cttcgtgctg
     9061 gccaccgcgc tgcgcgacgc cggtttcgag gtcaccgcgt tcgccgccgc cgccgaggcg
     9121 ctggacaccc tgcagcgcga catccaggcc aggcgcggcg caccggcgct ggtagtcacc
     9181 gacgtgcgca tgcccggcga cagcggcctg caactgctcg agaagttgaa ggcggcgaaa
     9241 cccgacctgc cggtgatcgt gatgtccgcg cacaccgacg tcgccagcac cgccggcgcg
     9301 ttccgcggcg gcgcccacga attcctgtcg aagccgttcg acctcgacga ggcggtggcg
     9361 ctggtccgtc gcgcgctgcc gcaacgcgat gcacgcggcc cgcgcgatgc gcaactgccg
     9421 gtcgatgcag gcgacgccga tgccgccacc ctggtcggcg atgcgccggc gatgcgcgcg
     9481 ctgttccgcg cgatcgggcg gctggcgcag gcgccgctgt cggtgctgat caccggggag
     9541 accggtaccg gcaaggaact ggtggcgcgt gcgctgcatc gggagtcgcc gcgtgcgtcg
     9601 cgtccgttcg tcgcgctcaa caccgcggcg atcccggccg agctgctgga atcggaattg
     9661 ttcggccacg aggcaggcgc gttcaccggc gcccacaaac gccatatcgg ccgcttcgaa
     9721 caggccgacg gcggcaccct gttcctcgac gagatcggcg acatgccggc gccgttgcag
     9781 acccggctgc tgcgggtgct ggccgagggc gagttcttcc gggtcggcgg gcgcgaactg
     9841 atccgggtcg acgtgcgggt gatcgcggcc acccaccagc cgctgcaggc gctggtcgag
     9901 gcgggccgct tccgcgccga cctgctgcac cgcctcgacg tggtgcggct gccgctgccg
     9961 ccgttgcgcg aacgccgcga ggacatcccg caactggccg cgcgcttcct cgccaatgcc
    10021 gcgcggcggc tgcagctggc accgaagaag ctgtccgccg ccgcgctgga gcgcctgcgc
    10081 gggcacgact ggccgggcaa cgtgcgtgag cttgagaacc tgtgctggcg gctggcggca
    10141 ctggccccgg gcgccagcat cggggtcgcc gacctcgacg aggcgatgcc ggcggcgcgc
    10201 gcggtggcgg acgccggcgg ccactggcag caggcgctgg cggcgtgggc gcgcgcgcaa
    10261 ctcgacgccg gcgtgcacga cctgcatgcc gccgcccgcc gcgagttcga ccgggtgctg
    10321 ctgcaggccg cgctcgagca cagcgagggc catcgtggcg aggccgcgca caggctcggg
    10381 ctgggccgca acacggtcac ccgcaagctg ggcccgtcgc gtacccggcg ctgagggttt
    10441 catcgcacct gaacccgcgg cgggcgagac tgccgggccc gcaccccgca agaccgagga
    10501 ccgcgcaatg acgcacgcca ccccgacccg cctggtcgcg acgatcgcct gttcgctgct
    10561 ggccgcctgc gccagcacca cccccgcgcc ggcaccgaag ccggccaccg gcaccgccca
    10621 ggaagcggtc gtcgtgctcg cgccggcgtc cggctcgctg gtcagcggca agctggtgct
    10681 gcgcccgcag ggcgatggcg tgcacatccg cggcgaagtc ggcggactca aggcgggcga
    10741 cgtccgcggg ttccacatcc acgaaagagg cgactgcagc gcggccgacg cctccagcgc
    10801 gggcggccac ttcaacccgg ccggcagtgc gcatggccgc gccgggcagg gcgcgcacca
    10861 tgccggcgac accgacaaca tcgtcgccga tgcccgcggc gtggcccgca tcgacgcgca
    10921 cgtgcgcggg gtgaccctgg gcggtggcgc ggccaatgac atcgccggcc gcgcactgat
    10981 cgtgcatgcg gcggccgacg actattcgac gcaacccacc ggcaatgccg gtgggcgggt
    11041 ggcgtgcggg gtggtgcggg tgctgcgcta gctagcgcag gcaatcggcg ttgccgtccg
    11101 cgcagtgcac gaactgcacc gggacggcgt gcgcctccag caccgcggcg aactgccgct
    11161 ggcgcgcctg gaaatattcg aagccgcgtt cgagcgcgct gcagtcggcc gcgcccggcg
    11221 cgtagcgggc gtgccaggca ccgggatcga acagggcgat gcggacctcg ccatcggcaa
    11281 agtgcgccag cggctccggg ccggcatcca gccaggactc ggccgcgggt tccagcagcg
    11341 cggtttccag caggctccac aacggctcca ggccgacgtt gcggtactgc agggcgatca
    11401 tcgcgagcag gtcgtgcagg ctgaggtagc gtgcatgctc gacctgcgcg gcaaggccat
    11461 cctgcagggc gagtgcggtg tccgccgcgg ccatgccgcg atccagcagc aggccttcca
    11521 gctcggcgcc cgcggcaacc gcgggcgcgc cgcgcagcac gaacggcagc acccgcaagc
    11581 cgccgccggc gaggttcggg tcgcactgca gcggcactgg aacatcgccc cgcgcatccg
    11641 caccgaaacc gaccatgcgc gcgccctggg cctggccggg tgcgcgctgc agcagttcca
    11701 gcgcgcgccg gtgcagcggc cagcccgggc gcagggcctc ggccggatcg aaatgcgcag
    11761 cgagcagggc gaggtcgcag tcgcgcacgc cgggtaccag tgcggccagg tcgcggccga
    11821 gcagcgcggt cgccgcccct gcgtcgccgg cgtccagcgc ggcacgcccg ggcggggcgc
    11881 cgtcgcgcag ttccagcgcc actacccctc ggaatacctg ctgccccgtg tcgtccatcg
    11941 atcctgagtt cccgctcgat atgtcggcgt tacactcgga ttatcgatga agaaagcccg
    12001 cggcgcgggc gaagcgagtc ccccatgctg cacggtcgtc ctgtcgccat ccttggtggt
    12061 gtccgcatcc cgttctgccg gcagaacacg gcgtatgccg atgtcggcaa cctcggcatg
    12121 tcggtgcgca cgctgggcgc gctggtcgag aagttcggcc tgcacgggca gcagctcggc
    12181 gaagtggcga tgggcgcggt gatcaagcat tcgtcggact ggaacctggg ccgcgaggcg
    12241 gcgctgtcct ccgggctgtc ggcgctgacc ccgggcatca ccctgcagcg cgcctgcggc
    12301 acgtcgctgg acacgatcat ccacatcgcc ggcaagatcg ccaccgggca gatcgaatcc
    12361 ggcatcggcg gcggttccga caccacctcc gacgtgccga tcgtgtacgg ccagtcgctg
    12421 cgccggcgcc tgctgaaggc cgccgcggcg aagaccaccg gcgacaagct ggccgcgttc
    12481 aagggcttca agctgtccga actgaagccg gatttcccgg gcgtggccga gccgcgcacc
    12541 ggcaagtcga tgggcaacca ctgcgaggac atggcgaagg agtggaacat ctcccgcgat
    12601 tcgcaggacg agctggccgt cgcctcccac cacaagctgg ccgcggccta cgagcgcggc
    12661 ttcttcgatg acctggtggt cagcttccgt ggggtctcgc gcgacaacgt gctgcgcccg
    12721 gacagcagcc tggagaagct gtccacgctc aagccggcgt tcgacaagag ctccggccgc
    12781 ggcaccctca ccgccggcaa ttcgaccccg ctgaccgacg gtgcttccgc ctgcctgctg
    12841 gccagcgacg agtgggcggc cgcgcacggc cacgaagtgc tgtgccattt gcgtgacgcg
    12901 caggtcgccg cggtcgattt cgtgcacggc gaaggcctgc tgatggcgcc aacggtggcg
    12961 gtggccgaga tgctcaagcg caacgggctc acgctgcagg atttcgattt ctacgagatc
    13021 cacgaagcct tcgccgcgca ggtgctgtgc accctgcggg cgtgggagag cgaggactac
    13081 tgcaggaccc gccttggcct cgaggcgccg ctgggccgca tcgacccggc gaagatcaac
    13141 cccaacggtt cgtcgctggc cgccggccac ccattcgcgg cgaccggtgc gcgcatcgtc
    13201 gccgcggccg cgaaggaact gaagcttcgc ggcggtggcc gttgcctggt ctcgatctgc
    13261 accgccggcg gcatgggcgt ggtcgcgatc ctggagcgtt gaggttccgc agcgccggac
    13321 gcaaagggcg aaccgatgcg cgacatcgat gacgggatgg ttccacctgc ggcaaggaga
    13381 ggaaagcaac gccatcgcgc ccgtctgatc ggcctgttgg cgttgcttgt cgcagtacct
    13441 tcggtgaccg tcggcagcga ccgcgaaccc gcactgcgca tgctgttcgt gggcaacagt
    13501 tacctgtacg tcaacgacct gccatcgcag gtgcgccgga tggcggccag caggggcgtg
    13561 gcgctggaca ttcgcatgtt ggcgcagccc gatttcgcgc tgtcagacca tctgcgggga
    13621 ccggatttcc tccgagagct ggatcgagat tgggactgga tcgtgttgca gcaagggccg
    13681 tcctcattgc ccgaaagcag ggaagacttg atcgccagcg tccgggtggt cgcggcccgt
    13741 gtgcagggca agcccgttcg ggtcgcgctc atgtcgggct ggcctgcgaa gcaatacgcg
    13801 gcccattcga gccagggcga agtcagctac cgattggcgg cgacgcatat cggcgcatgc
    13861 gtcatgccgg tcgccaccgc ctggcggttg gcgcgtgaga gcgacaagtc caccacgctc
    13921 tacgcgccgg acagattgca tgcgacgcgt gctggcaccc gccttgctgc aatggtggtc
    13981 ttgcatggct tgctaggcga gggtccgcag cccgccacgc aagatcgatc cgacactcca
    14041 gcgaagccga agcgagtcga cgtgttggag caggccgtca ttcgtgccta tgaagatgag
    14101 cccaaagggt gcgggccgga ctaatgcgat cggctgccca ctcgggaagc gccggttgct
    14161 gatctcaggt gcggcgaagg atgctgcgcg ccactgcttc ggcggtcttg atgccatcca
    14221 cgccggccga caggatgccg cccgcgtaac ccgcgccttc gccggccggg aacaggccgc
    14281 gggtgttgcg gctgtgaccg tcctcgccgc gggtgatgcg cagcggtgac gaagtgcgtg
    14341 tctccacgcc ggtcagcagc gcgtcctcca tcgcgaagcc ccggatctgc cgctcgaacg
    14401 cgggcagcgc ctcgcggatg gcgtcgatgg cgtagcgcgg cagcgcttcg tccagtgaac
    14461 ccaatgtcac gcccggcgtg tacgacggca gcaccgcacc gaaccgcgtc gaggggcggt
    14521 tgcggatgaa gtcgccgacc cgttgcgccg gcgccaggta atcgccgccg ccgagtgcgt
    14581 aggcctgcga ttcccaatgc cgctgcaggg cgatgccggc cagcggaccc tcgccatggg
    14641 gcgtgtaatc ctccggcgtg atcccgacca cgatgccggc gttcgcattg cgttcgttgc
    14701 gcgagtactg gctcatgccg ttggtgacca cgcggccgg
//
LOCUS       DAORUK010000046        14527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_3988_length_14527_cov_18.519900, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000046 DAORUK010000000
VERSION     DAORUK010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 14527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14527
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3988_length_14527_cov_18.519900"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..961)
                     /locus_tag="PK818_04710"
     CDS             complement(2..961)
                     /locus_tag="PK818_04710"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HQY82072.1"
                     /translation="MQDERNLLVFSGNANRPLASSVCKELGVRQGKALVSRFSDGEVQ
                     IEIEENVRGQDVFVIQPTCAPAAENLMELLALVDALKRASAGMVTAVVPYFGYARQDR
                     RPRSSRMPITAKVAASMFSAVRVDRVLTVDLHADQIQGFFDIPVDNVYASPLLLADIW
                     RAHGTDNLIVVSPDVGGVVRARAIAKRLDDADLAIIDKRRPRANVATVMNIIGDVEGK
                     TCVLVDDIVDTAGTLCAAANALKERGAHKVAAYCTHPVLSGAAIDNITRSQLDELVVT
                     DTIPLGEAARACGRIRQLSVAELLAETIRRVAFGESVSSLYVD"
     gene            complement(1093..1168)
                     /locus_tag="PK818_04715"
     tRNA            complement(1093..1168)
                     /locus_tag="PK818_04715"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1133..1135),aa:Gln,seq:ttg)
     gene            complement(1147..2058)
                     /gene="ispE"
                     /locus_tag="PK818_04720"
     CDS             complement(1147..2058)
                     /gene="ispE"
                     /locus_tag="PK818_04720"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HQY82073.1"
                     /translation="MNRFPGTEGWGTWPAPAKLNLFLQITGRRADGYHDLQTVFRLLD
                     WGDTVFLRPRADAVVARVGAGAAGVSEADDLLVRAAKLLQNESKCRQGVDIGIEKRVP
                     MGGGFGGGSSDAATVLVALDQLWGLHFGRERLAALGLRLGADVPVFVHGRNAWAEGVG
                     ERLAPIDLPPAWYVLASPGVHAATAGLFQAPDLTRNAAPAKMSDFLTAIALGNAAVPG
                     NAFEPVLRRREPAVEALFAVLARIGTPRLTGSGSGCFVEFATRESAEAALAALPPGLD
                     AWLARGVDRSPLLDAVESFNTGASPSG"
     gene            complement(2072..2704)
                     /gene="lolB"
                     /locus_tag="PK818_04725"
     CDS             complement(2072..2704)
                     /gene="lolB"
                     /locus_tag="PK818_04725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HQY82074.1"
                     /translation="MRPAAMAALACLLLAGCRTIAPPPDSTAAAAAQQARATTLGLAN
                     GDCAAPGWALAGRVALSNGRDGGSGQLEWTQGGGRTHLLLSAPITRQSWMLDVDPRGG
                     ATLQGLPEGSLGGDDAAQLVRDATGWDIPVAALGCWLRGVAAAEVALGVARIDFGADL
                     LPLRIEQGGWTVEYSGWKPDPFSRLPMPARINARRGEDRVRLVVDRWGLE"
     gene            complement(2701..4380)
                     /locus_tag="PK818_04730"
     CDS             complement(2701..4380)
                     /locus_tag="PK818_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011260047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY82075.1"
                     /translation="MPTPPRPHFRLLACAGLLACAFAAQAARLPQAAPVDPLEASIAG
                     EFALQAGQLGDAARRYLQAARSASDPVLAERATRIALLANEDALAREGYALWQALAPR
                     QSAPRQAVAATLALRAGEKRAARRELRALMGAGNDGWKHVLAALIGAVGTQPKLVVAM
                     LAGLVDDGELPDALEPWLGFGGLAQRLEQPALVDRIVREVVARFPGEPRVALLRAQLL
                     REDGKLGQARPLLAGLEDAARSSPQLRWSLAGEYDALGDPAKAAEILARGPQDEATHA
                     QRAALLDKAGDKAGLAALYAELRREATHPSPLRRLLLGQVAELLERRDEALGWYANVP
                     GEEAQGIARLRSANVMFQLQRKQEAFAALRAMQSEAALDEDARRDAYLLEAELRLKDK
                     DAAGELDAYARGLAAFADDAALLYSRALLWERRDDIARAEADFRRILVAAPDDVNALN
                     ALGYTLADRTTRYREALELIDRARVAEPGNAAIIDSYGWVLFRLGKPREALGHLRHAF
                     ALQKDADIASHLGQVLWVLGEREEARKYFDEARRIDPENRALQRALKEVGA"
     gene            4466..5737
                     /gene="hemA"
                     /locus_tag="PK818_04735"
     CDS             4466..5737
                     /gene="hemA"
                     /locus_tag="PK818_04735"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HQY82076.1"
                     /translation="MSLHVLGINHQTAPVALREKVAFAPDALAAALASLRALPQVHEA
                     VLLSTCNRTELYAHTDDDGRALADWLAIHPDTGHDLHAYLYRHRDGDAVRHLFRVATG
                     LDSLVLGEPQILGQVKQAWAASRDAGTLGGQLDRLFQQAFATAKRARTDTRIGANPVS
                     VASTAVRLAQESFARLSDSSVLLIGAGETIELAARHLVEARVQRLLIANRTLAHAQDL
                     ASRHGGVALPLAELDRHLAEADIVISATASREPVLHRAQVEHALRQRRHRPMLLLDLA
                     VPRDIASDVSGLRDVFLYTVDDLERAIEDNRRGRREAAEQAEAIVELQTARFVEALQA
                     SSRQQPILRLRAHGDAARADALAKARQQLAAGASPQQALEFLAHTLTNRLLHAPTVAL
                     REAALSGDGELPRAAEKLFPDASAGSGDTDG"
     gene            5734..6819
                     /gene="prfA"
                     /locus_tag="PK818_04740"
     CDS             5734..6819
                     /gene="prfA"
                     /locus_tag="PK818_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019186101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HQY82077.1"
                     /translation="MTPALRRKLDALVERREEVERLLADPAVAADQARFRNLSREFAT
                     LQPVADALASEARARADLAAAEAMRADPELRELADDEIEAATRRLQQLEGELLAQLVP
                     KDARDDGGLYLEVRAGTGGDEAAIFAGDLFRMYSRYAETRGWRVEVESANPGEHGGYK
                     EIVARVEGDGAYARLKYESGTHRVQRVPATEAQGRIHTSAATVAIIALEAGDFDITIN
                     PADLKVDTFRSSGAGGQHVNKTESAIRITHLPTGVVVESQTERSQHANRDKAMKRLQA
                     MLVEAELDKRAAAQAADRKLQVGSGDRSQRIRTYNFPQGRITDHRVEGLTLYDLPNII
                     EGDLDPLVLRLQQEQQAGELARLAREN"
     gene            6825..7280
                     /locus_tag="PK818_04745"
     CDS             6825..7280
                     /locus_tag="PK818_04745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY82078.1"
                     /translation="MSISTHRAGPADLDALALLFDGYRQFYGQASDVPRAREWLRERL
                     RFGESVVLVATRAGKPVGFVQLYPMFSSVRTARTWILNDLYVDAGARRGGVGRALLDA
                     AAKFARADGAAGISLETGRDNAAARALYRAAGWNEDATQWYSLPLSRSH"
     gene            7367..7699
                     /locus_tag="PK818_04750"
     CDS             7367..7699
                     /locus_tag="PK818_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017911650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82079.1"
                     /translation="MALATTLRLLSKASGLTVVDIATAIPRAGVDTVNAWLRGEKLPG
                     KDQVDALARTFGVPAGALLSELASTLDPARTKGEHDLLAAYRRLNTRQQGALLEVARS
                     MQPRAMKK"
     gene            7696..8472
                     /gene="ppk2"
                     /locus_tag="PK818_04755"
     CDS             7696..8472
                     /gene="ppk2"
                     /locus_tag="PK818_04755"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HQY82080.1"
                     /translation="MSKLKRKDYEELLVPLQEELAALARWLQHTGKRALVLFEGRDTA
                     GKGTAIDAIREHINPRQCRVAALPRPSERESSQWYFQRYVPHLPAAGEMVLFDRSWYN
                     RAGVEKVMGFASDAQVKAFMRDAPLFEKMLVDDGILLFKYWLCCDQEQQEKRFVERLR
                     DPLKRWKLSPIDVEARKRYGDYTRAREAMLKATHTAHAPWTLVDFNDQRRGRLTLIRN
                     LLDRLPDTRVDVPTIEFPPLPGKPARERYGVLKPIPTLKV"
     gene            complement(8480..8752)
                     /locus_tag="PK818_04760"
     CDS             complement(8480..8752)
                     /locus_tag="PK818_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HQY82081.1"
                     /translation="MAAVRFLVRGKVQGVWFRASTRAEALALGLRGHARNLADGGVEV
                     VATGDAAAIERLAAWLRHGPPTARVDTLQRFDADAADAAPEGFGVR"
     gene            complement(8752..9321)
                     /locus_tag="PK818_04765"
     CDS             complement(8752..9321)
                     /locus_tag="PK818_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011346527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQY82082.1"
                     /translation="MRVRMAVLLVLAMLAACTREPGPDPAAAPAAKPEPVQPAQPAQP
                     AGGADAVQQPALRVTTLDGNTFDLAGQRGKWVIVNFWATWCAPCLKEMPELSALDAMR
                     EHIVVVGLAYEETTTDELAAFMREHPVAYPIALVDVYDPPKDFAAPRGLPMTYLIAPD
                     GRVAKQYLGPVTARMIEEDIARAGGPKPV"
     gene            complement(9325..10596)
                     /locus_tag="PK818_04770"
     CDS             complement(9325..10596)
                     /locus_tag="PK818_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HQY82083.1"
                     /translation="MEPLDSLNRWSERLRDRARAGTFFRFTWKRFLGDRLFEAAGALS
                     FTTVFALVPLTMVVFGVLSAFPVFDEWSASLKAYIFANFVPRAASAVQGYLSEFSANT
                     RSLTTAGALALVVSLLVTLSSVEAIFNRIWRVATARPRFGRFLVYWTVLTLGGLVAAS
                     SLALSTRFFALAIFETVPGRWLEAVMLQLAPMAIEMLAFAAIFKVVPHRTVRWRHALA
                     GAALSVLLFELVKWGIGLYIGSFGSYQKIYGPLAFVPIFLLWIYLGWSAILFGASFAS
                     SMSAFRYQPAALRLPDGYELYGVLRMLGRFQQARGRGKGLHGDEIQQLEPILTDALVQ
                     ELLAKLNGIDVVRRAESGEWLLARDLDDVTLGDLYEAARLRVPVAEAHLPHREDALGV
                     AVMNTLDGLRMPLRDLLKQRVSSVFEGIEGD"
     gene            10718..11311
                     /gene="wrbA"
                     /locus_tag="PK818_04775"
     CDS             10718..11311
                     /gene="wrbA"
                     /locus_tag="PK818_04775"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010367335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HQY82084.1"
                     /translation="MPEILVLYYSRGGSVARLARQIARGVGEVEGMAARLRTVPPVAP
                     ITEVAAPPEPEQGAPYVDRRDLHECAGLLLGSPTRFGNMAAPMKHFLDTLGGEWASGT
                     LVGKPAAVFTSTATMHGGQESTLLTMLLPLLHHGCVVAGIPFTEPALSTTRSGGTPYG
                     ASHVAGPHDDSEPSDDEAQLARALGRRVAQLAAKLSA"
     gene            11308..11637
                     /locus_tag="PK818_04780"
     CDS             11308..11637
                     /locus_tag="PK818_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HQY82085.1"
                     /translation="MRSGRLLALALFAVAVLYGLWLGRGAHALAAMLVLVLPPLLLAV
                     AAWRGWPRAGFVAGVAALLWFSHGVMLAWSEPAQRMPALLLVVLALAVVFASSLDGIR
                     ARFGDTR"
     gene            11655..13214
                     /locus_tag="PK818_04785"
     CDS             11655..13214
                     /locus_tag="PK818_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQY82086.1"
                     /translation="MSGLPAGDVERLHAAARAIRDRTPAQAERLLGEVLARHPAHAEA
                     LRLLAILQLHTQRAPEAVATLQEAIARHPGDALLHADLGNAHSARGERDAAIAAWRRA
                     TGLDPTQPVPWFNLGRALQLEGDSHAAVAALETACALAPGLLPATILLGDALAHLGRF
                     DEATTRYRDALRQHPASGDAWRGLSNIKTVALADADAAALATQLQCRDIADSDRVAMG
                     HALGKLEEDRGRFPQAFAAFAAANALQQRLTPWRLPPLEAFVEQALAATATLPAPIDP
                     TLGHEVIFIVGLPRSGSTLFEQILAAHPQVEGASELPDLGIVIQQESVRRGVAYPQWV
                     PQASAADWQRLGRDYLARTARWRAARPRFTDKLPDNWKHAGVLRAMLPGATVLETRRD
                     PLETAWSCFKQQFYSQPHFANTVADIGACLRACERALDAWRARDPARIHLHRYEDLLA
                     EPEAAVRRLLADCGLGYDPACLRFHAATRSVRTASAAQVRQPLRRDTARAAAYGALLD
                     PLRHALGLPPA"
     gene            13261..13749
                     /locus_tag="PK818_04790"
     CDS             13261..13749
                     /locus_tag="PK818_04790"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HQY82087.1"
                     /translation="MDNLLIVTTGGTIDKVYFDDKSDFQVGEPQIGRILEELGVAFRF
                     SVIPILRKDSLHIDAADRALVRATIEAQDTRHVLVTHGTDSMVETARVLAAIAGKTIV
                     LTGALNPARFRGSDAEFNIGCAVGAVQSLPPGVYIAMNGRIWDPAKVRKNVDANRFEA
                     AG"
     gene            complement(13859..>14527)
                     /locus_tag="PK818_04795"
     CDS             complement(13859..>14527)
                     /locus_tag="PK818_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HQY82088.1"
                     /translation="LTNGVAKTNQSGAAGASLNYTMVVPAGATGLKFVTSGGTGDADL
                     YVKFGSAPTTTSYTCKSEGGTNAETCSIATAQAGTYYVLVKGYSAFSGMSITGSYTAG
                     GGGGGTQTYTNTTDVSIPDNNSTGVTSTIAVSGRTGNAPSTTAVAVNIVHTYSGDLVV
                     ELVAPDGTVYTLSNRVGGSADNIVKTYTVNLSSEAINGSWKLRAKDRAASDTGYINSW
                     SITM"
BASE COUNT         2197 a         5353 c         4989 g         1988 t
ORIGIN      
        1 gtcagtccac gtacagggaa ctgacggatt cgccgaacgc cacccggcgg atggtctcgg
       61 ccagcagttc ggcgacgctg agctggcgga tccggccaca ggcccgcgcc gcctcgccca
      121 gcgggatggt gtcggtcacc accagctcgt caagctgcga acgggtgatg ttgtcgatcg
      181 ccgcgcccga cagcaccggg tgggtgcagt aggccgcgac cttgtgcgcg ccgcgctcct
      241 tcagcgcgtt ggcggcggcg cacagggtgc cggcggtgtc gacgatgtcg tccaccagca
      301 cgcaggtctt gccctccacg tcaccgatga tgttcatcac ggtggcgacg ttcgcccgcg
      361 ggcggcgctt gtcgatgatg gcgaggtcgg catcatcgag gcgcttggcg atggcgcgcg
      421 cgcgcaccac gccgccgacg tccggcgaga ccacgatcag gttgtcggtg ccgtgcgcgc
      481 gccagatgtc ggcgagcagc agcggcgagg cgtagacgtt atccaccggg atgtcgaaga
      541 agccctggat ctggtcggca tgcaggtcca cggtcagcac ccggtccacc cgcaccgcac
      601 tgaacatcga cgccgccacc ttggccgtga tcggcatccg cgaagagcgc gggcgccggt
      661 cctggcgggc gtagccgaag tacggcacca ccgcggtcac catgccggcc gaggcgcgct
      721 tcagcgcgtc gaccagcgcc agcagctcca tcaggttctc cgccgccggg gcgcaggtgg
      781 gctggatcac gaacacgtcc tggccgcgga cgttttcctc gatctcgatc tgcacctcgc
      841 catcggagaa gcggctgacc agcgccttgc cctggcggac cccgagttcc ttgcacaccg
      901 agctggccag cggccggttg gcgttgccgg aaaacaccag caaattgcgt tcgtcctgca
      961 tcgttcgact ccgctccgct ctgaccgtgg cggtgatact cgggccgcac ggaggaccgc
     1021 gcggaaggga ctgcgaaaac ctttgccacg gaccatcgcc gacacgccgg ccgcgacgga
     1081 cccttcagga actggcaggg gcggaaggat tcgaacctac gcatgccagg atcaaaacct
     1141 ggtgccttaa ccgcttggcg acgcccctgt attgaaactc tccaccgcat ccagcagtgg
     1201 cgaccgatcg acgccgcgcg ccagccaggc atcgagcccc ggcggcaacg cggccagcgc
     1261 cgcctcggcg gattcgcggg tggcgaattc gacgaagcaa ccactgcccg accccgtcag
     1321 gcgcggcgtg ccgatgcgcg ccaggaccgc gaacaaggcc tcgacggcag gttcgcggcg
     1381 gcgcagcacc ggctcgaagg cattgcccgg aacagcggcg ttgccgagcg cgatggcagt
     1441 caggaagtcg gacattttcg cgggtgcagc attccgcgtc aaatcagggg cttggaacag
     1501 cccggcggtg gcggcatgga cacccggact ggccagcacg taccaggccg gcggcaggtc
     1561 gatcggggcc aggcgctcgc ccacgccttc cgcccaggca ttgcggccgt gcacgaacac
     1621 cgggacgtcg gcacccagcc gcaggccgag ggctgccagc cgctcgcggc cgaagtgcag
     1681 cccccacaac tggtccaggg ccaccagcac ggtcgcggca tccgacgaac cgccgccgaa
     1741 accgcccccc atcggaactc gcttttcgat gccgatgtcg acgccttgcc tacacttgga
     1801 ctcattttgc aggagtttcg cagcacgaac gaggagatcg tcggcttctg aaacgccggc
     1861 cgcgccggca ccgacgcgcg caaccactgc atccgcgcgc gggcgcagga acacggtgtc
     1921 gccccagtcc agcaggcgga atacggtctg caggtcgtgg tagccgtcgg cgcggcggcc
     1981 ggtgatctgc aggaacaggt tcagcttggc cggggccggc caggtgcccc agccttcggt
     2041 gcctggaaac cggttcatgg ccggtcgacg ctcattccag gccccagcgg tcgaccacca
     2101 gccgcacccg gtcctcgccg cgccgcgcgt tgatccgcgc gggcatcggc agccggctga
     2161 agggatccgg cttccagccg ctgtattcga cggtccagcc gccctgctcg atgcgcaggg
     2221 gcagcaggtc ggccccgaaa tcgatccgtg ccaccccaag cgcgacctcg gccgcggcga
     2281 ccccgcgcag ccagcaaccc agtgcggcaa ccgggatgtc ccagccggtc gcatcccgga
     2341 ccagttgcgc ggcatcgtcg ccacccagcg acccctcggg caggccctgc agcgtcgcgc
     2401 cgccgcgcgg atcgacgtcc agcatccacg actggcgggt gatcggcgcc gacagcagca
     2461 ggtgcgtgcg gccgcccccc tgcgtccatt cgagctgccc gctgccgccg tcgcggccat
     2521 tggacagcgc cacccgcccg gccagtgccc accccggcgc ggcgcagtcg ccgttcgcca
     2581 gccccagggt ggtggcgcgt gcctgctgcg cggcggcggc ggcggtggaa tccggcggcg
     2641 gcgcaatggt ccggcagccg gccagcagca ggcaggcaag cgccgccatg gcggccggcc
     2701 tcacgccccg acttccttca atgcgcgttg cagcgcgcgg ttctcggggt cgatccggcg
     2761 cgcctcgtcg aaatacttgc gcgcctcctc gcgctcgccg agcacccaca gcacctggcc
     2821 gaggtggctg gcgatgtccg cgtccttctg cagggcaaac gcgtggcgca ggtggcccag
     2881 cgcctcgcgc ggcttgccga ggcggaacag gacccagccg tagctgtcga tgatcgcggc
     2941 attgccgggc tcggccaccc gcgcacggtc gatcagttcc agcgcttccc ggtagcgggt
     3001 ggtgcgatcg gccagggtgt agccgagcgc gttcagggcg ttcacgtcat ccggcgctgc
     3061 gaccaggatg cggcggaaat ccgcttccgc ccgggcgatg tcgtcgcggc gttcccacag
     3121 cagcgcgcgc gaatacagca gggcggcgtc atcggcgaac gcggccagcc cgcgcgcata
     3181 ggcatccagt tcgccggcgg catccttgtc cttgagccgc agctcggcct ccagcaggta
     3241 ggcatcgcgg cgcgcatcct cgtccagcgc ggcttcggac tgcatcgcgc gcagcgcggc
     3301 gaacgcctcc tgcttgcgct gcagctggaa catcacgttc gcgctgcgca ggcgggcgat
     3361 cccctgcgcc tcctccccgg gcacgttcgc gtaccagccc agggcctcgt cgcggcgctc
     3421 cagcagttcc gcgacctgcc ccagcagcag ccggcgcagc gggctcgggt gggtcgcctc
     3481 gcgcctgagt tcggcgtaca gcgcggccag cccggccttg tccccggcct tgtccagcag
     3541 cgccgcgcgc tgtgcgtggg tggcctcgtc ctgcgggccg cgcgccagga tctcggccgc
     3601 cttcgccggg tcgccgaggg cgtcgtactc gccggccagc gaccagcgca gctgcggcga
     3661 cgagcgcgcg gcatcctcca ggcccgccag caacggccgt gcctgcccga gcttgccgtc
     3721 ctcgcgcagc agctgcgcgc gcagcagcgc cacccgcggc tcgccgggga agcgcgccac
     3781 cacctcgcgg acgatgcgat ccaccagcgc cggctgctcc agccgctgcg ccaggccacc
     3841 gaagcccagc cacggttcca gcgcgtccgg caactcgccg tcgtcaacca gcccggccag
     3901 catcgccacc accagcttcg gctgggtgcc gaccgccccg atcagcgccg ccagcacgtg
     3961 cttccagcca tcgttgcccg cgcccatcag cgcccgcagt tcgcgcctgg cggcacgctt
     4021 ctcgcccgcg cgcaatgcca gcgtggccgc cactgcctgc cgtggcgcgc tttgccgcgg
     4081 ggccagcgcc tgccacagcg cgtagccctc gcgggccagt gcgtcctcgt tcgcgagcag
     4141 ggcgatgcgg gtggcgcgct ccgccagcac cgggtcgctg gcgctgcgcg cggcctgcag
     4201 gtagcggcgc gcggcgtccc ccaactgccc cgcctgcagg gcgaattcgc cggcgatgct
     4261 ggcttccagc gggtccacgg gcgcggcctg cggcaggcgc gccgcctgcg ctgcgaatgc
     4321 gcaggccagc aacccggcgc aggccagcaa gcggaaatgt gggcgtggtg gcgtcggcat
     4381 gcagattctt gtttgaagcc cggtcggtac aatatcgggt cgtgccggca gcttagcgca
     4441 ggcacctgaa cggatttcgc gatcgatgag cctgcacgtc ctcggcatca accaccagac
     4501 cgcgccggtt gccctgcgcg agaaggtggc gttcgccccg gacgcgctgg ccgccgcgct
     4561 ggcctcgctg cgggcgctgc cgcaggtgca cgaggcggtg ctgctgtcca cctgcaaccg
     4621 caccgagctg tacgcgcaca ccgacgacga tggccgcgcg ctggccgact ggctggcgat
     4681 ccacccggac accggccacg acctgcacgc ctacctgtac cggcaccgcg acggcgacgc
     4741 cgtccgccac ctgttccggg tcgccaccgg gctggattcg ctggtgcttg gcgagccgca
     4801 gatcctgggc caggtcaagc aggcgtgggc ggcctcgcgc gatgccggca cccttggcgg
     4861 ccagctcgac cgcctgttcc agcaggcctt cgccaccgcc aagcgcgcgc gcaccgacac
     4921 ccgcatcggc gccaacccgg tgtcggtggc gtccactgcg gtgcgcctgg cgcaggaatc
     4981 gttcgcccgc ctcagcgatt ccagcgtgct gctgatcggc gccggcgaga ccatcgagct
     5041 ggccgcgcgg cacctggtcg aggccagggt gcagcggctg ctgatcgcca accgcacgct
     5101 tgcccacgcc caggacctgg ccagccgcca tggcggcgtc gccctgccgc tggccgagct
     5161 ggaccgccac ctggccgagg ccgacatcgt gatctccgcg accgccagcc gcgaaccggt
     5221 gctgcaccgg gcgcaggtgg agcatgcgtt gcgccagcgc cggcaccggc cgatgctgct
     5281 gctcgacctc gcggtgccgc gcgacatcgc cagcgacgtg tccggcctgc gcgacgtgtt
     5341 cctgtacacc gtggacgacc tcgaacgcgc gatcgaggac aaccgccgcg gccgccgcga
     5401 agccgcggag caggccgagg cgatcgtcga gctgcagacc gcgcgcttcg tcgaggcgct
     5461 gcaggccagc agccgccagc agccgatcct gcgcctgcgc gcgcatggcg atgccgcgcg
     5521 cgccgacgcg ctggccaagg cccggcagca gcttgccgcc ggcgcctcgc cgcagcaggc
     5581 gctggaattc ctggcgcaca ccctcaccaa ccggctgctg cacgcaccca cggtggccct
     5641 gcgcgaggcc gcgctcagcg gcgacggcga actgccgcgg gcggcggaga agctgttccc
     5701 ggacgccagc gccggcagcg gcgacaccga cggatgacgc ccgcgctgcg ccgcaagctc
     5761 gacgccctgg tcgagcgccg cgaggaagtc gaacgcctgc tcgccgatcc ggcggtggcg
     5821 gccgaccagg cgcgtttccg caacctctcg cgcgagttcg cgaccctgca gccggtggcc
     5881 gacgcgctgg cgtcggaagc gcgcgcgcgc gccgacctgg ccgccgccga agcgatgcgc
     5941 gcggacccgg aactgcgcga actggcggac gatgaaatcg aagcggcgac gcgccgcctg
     6001 cagcagctgg aaggcgaact gctggcgcag ctagtgccga aggacgcgcg cgacgacggc
     6061 ggcctgtacc tggaagtgcg cgccggcacc ggcggcgacg aggcggcgat cttcgccggc
     6121 gacctgttcc ggatgtacag ccgctacgcg gaaacgcgcg gctggcgggt cgaggtggaa
     6181 tccgccaatc cgggcgaaca cggcggctac aaggagatcg tggcccgcgt cgaaggcgac
     6241 ggcgcctacg ccaggctcaa gtacgaatcc ggcacccacc gcgtgcagcg ggtaccggcg
     6301 accgaggcgc aggggcgcat ccacacctcg gcggcgacgg ttgcgatcat cgcgctggag
     6361 gccggcgact tcgacatcac gatcaatccc gccgacctca aggtcgacac cttccgcagc
     6421 tccggcgccg gcggccagca cgtcaacaag accgaatcgg cgatccgcat cacccacctg
     6481 cccaccggcg tggtggtgga atcgcagacc gagcgcagcc agcacgccaa ccgcgacaag
     6541 gcgatgaagc gcctgcaggc gatgctggtg gaagccgaac tggacaagcg cgccgccgcg
     6601 caggccgccg accgcaagct gcaggtgggc agtggcgacc gcagccagcg catccgcacc
     6661 tacaacttcc cgcagggccg gatcaccgac caccgggtgg agggactcac cctctacgac
     6721 ctgcccaaca tcatcgaagg cgacctcgac ccgctggtgc tgcgcctgca gcaggaacag
     6781 caggccgggg aactggcgcg cctggcccgc gagaactgac ccccatgtcg atttccaccc
     6841 atcgcgccgg ccccgccgac ctcgacgccc tcgccctgct gttcgatggc taccggcagt
     6901 tctacggaca ggccagcgac gtgccgcgcg cgcgcgagtg gctgcgcgaa cgcctgcgct
     6961 tcggcgaatc ggtggtgctg gtggcgaccc gcgccggcaa gccggtcggc ttcgtccagc
     7021 tctatccgat gttctcctcg gtgcgcaccg cgcgcacctg gatcctcaac gacctctacg
     7081 tcgatgccgg cgcacgccgc ggcggcgtcg gccgcgcgct gctggatgcc gcggcgaagt
     7141 tcgcgcgcgc cgatggcgcc gccggcattt cgctggaaac cggccgcgac aacgccgccg
     7201 cgcgcgccct gtaccgcgcc gccggctgga acgaagacgc cacccagtgg tattcgctgc
     7261 cactctcgcg cagccattga tgcagcgggc cgtggccggc cctaaactcg atgccggggc
     7321 gcctccggaa cctgcccgcc aaccagccgc gcctgaagga gtgacgatgg ccctcgcgac
     7381 caccctgcga ttgctcagca aggccagtgg cctgaccgtg gtcgatatcg ccaccgccat
     7441 cccgcgcgcg ggggtggaca cggtcaatgc ctggctgcgc ggcgagaaac tgcccggcaa
     7501 ggaccaggtc gatgcgctcg cgcggacctt cggcgtcccc gccggcgcgt tgctttcgga
     7561 actggccagc accctggatc cggcgcgcac caagggcgag cacgacctgc tggccgccta
     7621 ccgccgcctc aacacgcggc aacagggcgc gctgctggaa gtggcgcgca gcatgcagcc
     7681 cagggccatg aagaaatgag caagctcaag cgcaaggact acgaggaact gctggtcccg
     7741 ctgcaggagg aactggcggc gctggcgcgc tggctgcagc acaccggcaa gcgcgcgctg
     7801 gtgctgttcg agggccgcga caccgccggc aagggcaccg cgatcgatgc gatccgcgag
     7861 cacatcaacc cgcgccagtg ccgggtcgcg gcattgccca ggccgagcga acgcgaatcc
     7921 agccagtggt acttccagcg ctacgtgccg cacctgccgg cggccggcga aatggtgctg
     7981 ttcgaccgca gctggtacaa ccgcgccggc gtcgagaagg tgatgggctt cgccagcgat
     8041 gcgcaggtga aggcgttcat gcgggacgcc ccgctgttcg agaagatgct ggtcgatgac
     8101 ggcatcctgc tgttcaagta ctggctgtgt tgcgaccagg agcagcagga aaagcgcttc
     8161 gtcgagcgcc tgcgcgaccc gctcaagcgc tggaagctct cgccgatcga cgtcgaggcg
     8221 cgcaagcggt acggcgacta cacccgcgcc cgcgaagcga tgctcaaggc cacccatacc
     8281 gcgcacgcgc cgtggacgct ggtggacttc aacgaccagc ggcgtggccg cctgaccctg
     8341 atccgcaacc tgctggaccg cctgccggac acccgggtgg acgtccccac gatcgaattc
     8401 ccgccgctgc cgggcaagcc ggcgagggaa cgttacggcg tgctgaagcc gatcccgacc
     8461 ctgaaggtgt aggcacgcgc tagcgcaccc cgaagccctc gggcgcggca tcggcggcat
     8521 cggcgtcgaa gcgctgcagg gtgtccaccc gcgccgtcgg cggcccgtgc cgcagccacg
     8581 ccgccagccg ttcgatggcc gcggcatcac cggtcgccac cacctcgacg ccgccatccg
     8641 caaggttgcg cgcgtgcccg cgcagcccca gcgccagcgc ttcggcgcgg gtactggcgc
     8701 ggaaccagac gccctggacc ttgcccctca ccaggaaccg caccgccgcc atcagaccgg
     8761 cttggggccg cccgcgcggg cgatgtcttc ttcgatcatc ctggcggtga ccgggcccag
     8821 gtactgcttg gcgacacggc catccggcgc gatcaggtag gtcatcggca aaccccgcgg
     8881 ggccgcgaaa tccttcggcg ggtcgtacac gtcgaccagc gcgatcggat acgccaccgg
     8941 atgctcgcgc atgaacgccg cgagttcgtc ggtcgtggtt tcctcgtagg ccaggccaac
     9001 caccacgatg tgctcgcgca tcgcgtcgag tgccgacaac tccggcattt ccttcaggca
     9061 cggcgcgcac caggtcgccc agaagttgac gatcacccac ttgccgcgct gcccggccag
     9121 gtcgaaggtg ttgccgtcga gggtggtgac gcgcaacgcc ggctgctgca cggcatctgc
     9181 gccgcccgcg ggttgcgccg gttgcgccgg ttgcacgggt tccggcttcg cagccggcgc
     9241 cgcagcagga tccggcccgg gctcgcgcgt gcacgccgcc aacatcgcca gcaccagcaa
     9301 taccgccatc cgcacacgca tcgatcagtc tccttcgatt ccttcaaaaa cactggatac
     9361 gcgctgcttg agcaggtcgc gcaggggcat ccgcaagccg tcgagggtgt tcatcaccgc
     9421 gacgccgagg gcgtcctcgc ggtgcggcag gtgcgcctcg gccaccggca cgcgcaggcg
     9481 cgcggcttcg tacaggtcgc caagggtgac atcgtcgagg tcgcgcgcca gcagccattc
     9541 gccgctctcg gcgcgccgga ccacgtcgat cccgttgagc ttcgcgagca gttcctgcac
     9601 cagcgcgtcg gtcaggatcg gctccagctg ctggatctcg tcgccgtgca ggcccttgcc
     9661 gcgcccccgc gcctgctgga agcgcccgag catgcgcagc acgccgtaca actcgtaccc
     9721 gtccggcagc cgcagcgccg ccggctggta gcggaacgcc gacatcgacg aggcgaacga
     9781 cgcgccgaac aggatcgcgc tccagcccag gtagatccac agcaggaaga tcggcacgaa
     9841 cgccagcggc ccgtagatct tctggtagga accgaagctg ccgatgtaca ggccgatgcc
     9901 ccacttcacc agctcgaaca gcagcaccga cagcgccgcc ccggccagcg catgccgcca
     9961 gcgcacggtg cggtgcggca ccaccttgaa gatcgcggcg aacgcgagca tctcgatcgc
    10021 catcggcgcc agctgcagca tcaccgcttc cagccatcgc cccggcacgg tctcgaagat
    10081 cgccagcgcg aagaagcggg tcgacaacgc caggctgctg gcggcgacca gtcctcccag
    10141 ggtcagcacg gtccagtaca ccaggaagcg gccgaacctc gggcgcgcgg tcgccacccg
    10201 ccagatccgg ttgaagatcg cctccacgct ggacagcgtc accagcagcg agaccaccag
    10261 cgccagcgcg ccggcggtgg tcagcgacct ggtgttggcg gagaactcgc tcaggtagcc
    10321 ctgcaccgcg ctcgccgcgc gcggcacgaa gttggcgaag atgtaggcct tcaggctggc
    10381 gctccactcg tcgaacaccg ggaacgcgga gagcacgccg aacaccacca tcgtcagcgg
    10441 caccagcgcg aacacggtgg tgaacgagag cgcacccgcg gcctcgaaca ggcggtcgcc
    10501 gaggaagcgc ttccaggtga agcggaagaa ggtgcccgcg cgcgcacggt cgcgcaggcg
    10561 ttcgctccat cggttgaggg aatccagtgg ttccattcgc gcaagcctaa ccgcgaacaa
    10621 ggccgggggt gaattcctgc agcatgcccg cggccgcccg ctcggtcgcg atggcttcat
    10681 gcgaaacttc cgcgcaggcc acttcgcaag cgatcccatg cccgagatcc tcgtcctcta
    10741 ctacagccgc ggcggatccg tggcgcggct tgcgcgccag atcgcgcgcg gtgtcggcga
    10801 ggtcgaaggg atggccgcgc gcctgcgcac ggtgccgccg gtggcgccga tcaccgaggt
    10861 cgcggcaccg ccggaacccg agcaaggcgc gccctacgtc gaccgtcgcg acctgcacga
    10921 atgcgcgggc ctgctgctcg gaagcccgac ccggttcggc aacatggcgg caccgatgaa
    10981 gcacttcctc gacaccctcg gcggcgaatg ggccagcggc accctggtcg gcaagccggc
    11041 ggcggtgttc acctccaccg cgaccatgca tggcggccag gaatcgaccc tgctgaccat
    11101 gctgctgccg ctgctgcacc acggctgcgt ggtcgccggg attccgttca ccgagcctgc
    11161 gctgagcacc acccgcagcg gcggcacgcc ctacggcgcc agccacgtgg ccggtccgca
    11221 cgacgacagc gaaccgagcg acgacgaagc gcagctcgcg cgcgcgctgg gccggcgcgt
    11281 tgcgcagctg gcggcgaagt tgtccgcatg aggtccggac gcctgcttgc gctcgccctg
    11341 ttcgccgttg ccgtgctcta cggcctgtgg ctggggcgcg gcgcgcatgc actggcggcg
    11401 atgctggtgc tggtgctgcc accgctgctg ctggccgtcg cggcctggcg cggctggccg
    11461 cgcgcgggct tcgtcgccgg cgtcgccgcc ctgttgtggt tcagccatgg agtgatgctg
    11521 gcgtggtcgg aacccgcgca gcgcatgccg gcgctgctgc tggtggtgct ggcgctggcg
    11581 gtggtgttcg cctccagtct cgacggcatc cgcgcgcggt tcggcgacac ccggtgaaca
    11641 cgcaggatcc acgcatgtcc ggcttgccgg cgggcgacgt cgagcgcctg cacgccgccg
    11701 cgcgcgccat tcgcgaccgc accccggcgc aggccgaacg cctgcttggc gaggtgctcg
    11761 cgcggcatcc ggcgcatgcg gaagcgctgc gcctgctggc gatcctgcag ctgcacacgc
    11821 agcgtgcgcc tgaggcggtg gcaaccctgc aagaggcgat cgcacggcac cctggcgatg
    11881 ccctgctgca cgcagacctc ggcaatgcgc acagcgcgcg tggcgagcgc gacgccgcga
    11941 tcgcggcgtg gcggcgcgca accggcctcg accccacgca accggtgccg tggttcaacc
    12001 tcggccgcgc gctgcagctg gagggcgaca gccacgccgc ggtcgccgcg ctcgaaaccg
    12061 cctgcgcact ggcgcccggg ctgctgccgg cgacgatcct gctcggcgac gcactcgcgc
    12121 acctgggccg cttcgacgag gcgacaaccc gttaccgcga cgccctgcgc cagcacccgg
    12181 ccagcggcga cgcctggcgc gggctgtcca acatcaagac cgtggcgctg gccgatgccg
    12241 acgcggcggc actggccacc cagctgcagt gccgcgacat cgccgacagc gaccgggtgg
    12301 cgatgggcca cgcactgggc aagctggagg aggaccgtgg ccgcttcccg caggcattcg
    12361 ccgcattcgc ggcggcgaat gcgctgcagc agcggctgac gccatggcgg ctgccacctc
    12421 tcgaggcgtt cgttgagcag gcgctggccg ccaccgcaac cctgcccgcg ccgatcgacc
    12481 ccaccctcgg ccacgaggtg atcttcatcg tcgggctgcc gcgttcgggt tcaaccttgt
    12541 tcgaacagat ccttgccgcg catccgcagg tcgagggcgc cagcgagctg ccggacctcg
    12601 ggatcgtgat ccagcaggag tcggtgcggc gcggggtggc ctacccgcag tgggtcccgc
    12661 aggccagcgc ggcggactgg cagcgcctgg gccgcgacta cctcgcgcgc acggcgcgct
    12721 ggcgggcggc gcgcccgcgc ttcaccgaca agctgccgga caactggaag cacgccggcg
    12781 tgctgcgcgc gatgctgccg ggcgcgaccg tgctcgagac ccgccgcgac ccgctggaaa
    12841 cggcgtggtc ctgcttcaag caacagttct acagccagcc gcatttcgcc aacaccgtgg
    12901 ccgacatcgg tgcctgcctg cgcgcctgcg agcgcgcgct ggacgcgtgg cgcgcgcgcg
    12961 atccggcgcg catccacctg catcgctacg aagacctgct ggccgaaccc gaggccgcgg
    13021 tgcgaaggct gctggccgat tgcgggctgg ggtacgaccc ggcctgcctg cgcttccatg
    13081 ccgccacccg cagcgtgcgc accgccagcg ccgcacaggt gcggcagccg ttgcggcgcg
    13141 acaccgcgcg cgcggcggca tatggcgcgt tgctggaccc gctgcggcac gcacttggcc
    13201 ttccgcctgc ctgacgccgg cgccggcttg gcggataatg gcgttttgga gcgggcgcgc
    13261 atggacaacc tgctgatcgt caccaccggc ggcaccatcg acaaggtcta tttcgacgac
    13321 aagtcggact tccaggtcgg cgaaccgcag atcgggcgca tcctcgagga actgggggtc
    13381 gccttccgct tcagcgtgat cccgatcctg cgcaaggact cgctgcacat cgacgccgcc
    13441 gaccgcgcgc tggtgcgcgc caccatcgag gcgcaggaca cccgccacgt gctggtcacc
    13501 cacggcaccg acagcatggt cgagaccgcg cgggtgctgg cggcgatcgc cggcaagacc
    13561 atcgtcctca ccggcgccct gaatccggcg cgcttccgcg gctccgatgc cgagttcaac
    13621 atcggctgcg cggtcggcgc ggtgcagtcg ctgccgcccg gtgtgtacat cgccatgaac
    13681 ggccgcatct gggacccggc gaaggtgcgc aagaacgtcg acgccaaccg cttcgaggcg
    13741 gccggctgaa gccacggccg cgcgccgccg gccggcagcg cgcacccttc gcatcccgca
    13801 atgaaaaacc ccggccgaag ccggggtttt tcgcatgtgt cgacggcgat gccatcgatt
    13861 acatggtgat gctccagctg ttgatgtagc cggtgtccga cgccgcgcga tccttggccc
    13921 gaagcttcca gctgccgttg atggcttcgc tggacaggtt cacggtgtag gtcttgacga
    13981 tgttgtcggc gctgccaccg acgcggttcg acagcgtgta caccgtaccg tccggagcca
    14041 ccagctcgac caccaggtcg ccgctgtagg tgtggacgat gttgaccgcg accgcggtgg
    14101 tgctgggcgc gttgccggtg cggccggaca ccgcgatggt gctggtgacg ccggtgctgt
    14161 tgttgtccgg gatgctgacg tcggtggtgt tggtgtaggt ctgggtaccg ccaccgccgc
    14221 cgccggcggt gtagctgccg gtgatgctca tgccggagaa cgcgctgtag cccttgacca
    14281 gcacgtagta cgtgcccgcc tgcgcggtgg cgatgctgca ggtctcggca ttggtgccgc
    14341 cttccgactt gcaggtgtag ctggtggtgg tcggtgcgct gccgaacttg acgtacaggt
    14401 cggcgtcacc ggtgccgccg gaggtgacga acttcaggcc ggtggcgccc gccgggacca
    14461 ccatcgtgta gttcaacgag gcaccggccg caccggactg gttggtcttg gccacgccgt
    14521 tggtgag
//
LOCUS       DAORUK010000047        14435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_4025_length_14435_cov_17.385466, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000047 DAORUK010000000
VERSION     DAORUK010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 14435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14435
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4025_length_14435_cov_17.385466"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..868)
                     /gene="htpX"
                     /locus_tag="PK818_04800"
     CDS             complement(5..868)
                     /gene="htpX"
                     /locus_tag="PK818_04800"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012480842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HQY82089.1"
                     /translation="MMRVLLFIATNFAVMILLGAVLWVAENVFGLRLGTGNAGLLVMA
                     AVFGMGGAFISLALSKWMAKRATGAHVIDKPRNEAEAWLLATVQKQAQAAGIGMPEVA
                     IYDAPDLNAFATGMNRNKALVAVSTGLLRGMERREVEAVLGHEISHVANGDMVTLTLI
                     QGVLNTFVIFLSRIVGNIIDGMIRGNRDGGPGIFYFVIVMVLQVVFGIFASMIVAWFS
                     RRREFRADAGGAKLEGRDAMIAALERLKGNHEQTALPKEIAAFGISGGVGALFASHPP
                     LDVRIAALRAG"
     gene            998..1930
                     /gene="gluQRS"
                     /locus_tag="PK818_04805"
     CDS             998..1930
                     /gene="gluQRS"
                     /locus_tag="PK818_04805"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HQY82090.1"
                     /translation="MPHPSAYRGRFAPSPTGELHFGSLVAALGSWLRARAQGGAWLVR
                     IEDLDPPREVPGSGEGIVQALAAFGLHSDEAIVHQGDRSPSYAEALQRLVDIGAAFPC
                     HCSRAELGASGIHHACVAGPGPATREPAMREPAWRVRAPAQVIAFDDAIQGRYAQDLA
                     AAVGDYVVRRADGWWAYQLAVVVDDAWQGITEVVRGADLIDSTPRQIHLQRLLGLPTP
                     AYAHLPLALDASGAKLSKRSASLPVDPGDPLPALRAALAFLGQPLPAVPAAAGPAGLL
                     AAAARDFDLARIPRKSRTPAYADAQGAPTAAPHR"
     gene            1997..2737
                     /locus_tag="PK818_04810"
     CDS             1997..2737
                     /locus_tag="PK818_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014090372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HQY82091.1"
                     /translation="MESRVALVTGGTGGIGSAIVKRLARMGHRVATNYRDEAKARKWQ
                     EQARAEGLEILIVPGDVANPESAAAMVDAVRSALGPVDILVNNAGITRDTTFHRMSAL
                     QWQEVINTNLNSCFNVTRPVIDGMRERKWGRIIQISSINGQKGQYGQANYAASKAGMH
                     GFTISLAQENARFGITVNTVSPGYIATDMVMAVPEEVRAKIVVNIPTGRLGTPEEIAY
                     AVSFFMPDEAAWITGANLSVNGGHYMGW"
     gene            3087..3596
                     /gene="phaR"
                     /locus_tag="PK818_04815"
     CDS             3087..3596
                     /gene="phaR"
                     /locus_tag="PK818_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HQY82092.1"
                     /translation="MAKIRVIKKYPNRRLYDTEISSYITIEDVRQLVVDNEVFEVRDA
                     KSNADITRSVLLQIISEHEDRGTPMFSATVLSQIIRFYGDSLQGFVGSYLERSLQTFL
                     EQSQQFRSQLNNVIGQTPWTMLNEMTERNLDMWKSMQNRFLSAASSAVRANNAPPEAK
                     PAGKTERQR"
     gene            3711..4457
                     /gene="phbB"
                     /locus_tag="PK818_04820"
     CDS             3711..4457
                     /gene="phbB"
                     /locus_tag="PK818_04820"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HQY82093.1"
                     /translation="MTTRTAVVTGGLGGLGTEICKHLARAGRKVVAADLAGRADRVDE
                     FRRETAEFNGAIRFEDLDVTDEASCAELVRRAAGDSGGVDILVNAAGITRDGTLRKMS
                     SQQWRDVLTVNLDGVFHMTRQVVDGMCERGFGRIVNISSVNGQTGQFGQTNYSAAKAG
                     MHGFTMALAREVARKGVTVNSVSPGYCRTSLVMKIPEQIRNDIVSSIPVGRLGEPSEI
                     ARTVQFLSADDAGFITGANIPVNGGYFMDF"
     gene            4546..5487
                     /locus_tag="PK818_04825"
     CDS             4546..5487
                     /locus_tag="PK818_04825"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HQY82094.1"
                     /translation="MQQRQTKYERLLARARELPAMSTAIAHPCDEYSLAAALDAARIG
                     LIRPILVGPVARIRAAAAKAGLSLDGIEIVDTAHSHAAAEEAVRQVRLGKAECLMKGS
                     LHTDELMGAVLDRETGLRTSRRISHCFIMDVPGHDDVIIISDAAINIAPDLAAKVDIV
                     QNAIDLAHALGAGEVRVAILSAMETVNPKVPSTIEAAALCKMADRGQITGAILDGPLA
                     LDNAIDPEAARMKQIVSPVAGRANVLIVPDLEAGNMLAKSLTFLANADAAGIVLGARV
                     PIMLTSRADSVETRLASCAVASLVAKARRDALSRKVV"
     gene            5530..6711
                     /locus_tag="PK818_04830"
     CDS             5530..6711
                     /locus_tag="PK818_04830"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HQY82095.1"
                     /translation="MADAIAVLNAGSSSIKFSVYLHDAGRLEVALRGQVEGLHTAPRF
                     VARDRSGQVDAEKSWGEGVSLGHDGAMAHLVGYLRQRLGGSRLLGIGHRVVHGGMHFS
                     QPVRVDAQVLEMLRQFVPLAPLHQPHNLAPIGQMLESAPELPQVACFDTAFHRDNPEL
                     AQMFALPAELHAQGVRRYGFHGLSYEYIASVLPGIDARAASGRTVVLHLGNGASMCAL
                     EGARSVASTMGFTAVDGLLMGTRCGALDPGVILYLMDQRGMDARAIEQLIYSKSGLLG
                     VSGISSDMRTLLASDDPRARLAVELYAYRIRRELGSLAAALGGLDALVFTAGIGEHAA
                     PIRERVCRDAAWLGLVFDAAANAAHGPRISSAASRVSAWVVPTNEELMIARHTLALLA
                     A"
     gene            complement(6833..7180)
                     /locus_tag="PK818_04835"
     CDS             complement(6833..7180)
                     /locus_tag="PK818_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HQY82096.1"
                     /translation="MKHKGSCHCGRVAFEVEGEIKGAMACNCSICQRKGSLLWFVPRA
                     QLQLLTPEGAASTYTFNRHVIKHRFCPVCGIHPYGEGTDPKGNAMAAINIRCLDDIEL
                     STIPVQEYDGRSL"
     gene            complement(7232..7678)
                     /locus_tag="PK818_04840"
     CDS             complement(7232..7678)
                     /locus_tag="PK818_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82097.1"
                     /translation="MPKLLRNTLAVLAGLVLGGSVNMALITISPSLIPPPAGVDVTNA
                     ESLAASMHLFEPRHFIMPFLAHALGTLAGALVTYLFANSFRPQLAYVIGAVFLCGGVA
                     ASFMIPAPAWFIALDLLVAYLPMAWLGIRIGALLRKGTAGGPGANA"
     gene            complement(7908..8435)
                     /locus_tag="PK818_04845"
     CDS             complement(7908..8435)
                     /locus_tag="PK818_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82098.1"
                     /translation="MSAQDCSRHRILNCMSISNPRRAPSDFDFLIGDWHVQHRRLDSR
                     LSGCTDWTEFPGLSSTRKILGGFGNVEDNVLRFPDGEVRAAAFRSYNDASQEWAIWWL
                     DGRAPHHLDVPVVGRFDGPVGVFHAKDSLDGRPLDVRFIWTVNPGGNPRWEQAFSIDG
                     GTTWETNWVMVFVRQ"
     gene            complement(8452..9141)
                     /locus_tag="PK818_04850"
     CDS             complement(8452..9141)
                     /locus_tag="PK818_04850"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HQY82099.1"
                     /translation="MAEEFPVVFAVYPHVTQLDFTGPHEVFSRLPGARCILASSTGGD
                     LEADGGLVFARVQRLADVERCALLCVPGGFGTIAAMEDQELLAQVRRLAGTARYVTSV
                     CTGALVLGAAGLLKGKRATTHWAWREALPAFGAKPDSARVVRDGNVFTGGGVTAGIDF
                     ALTVLAEIAGAAFAQSVQLRIEYAPDPPFNAGRPELAPVQVLATAQARYEEVRSARDA
                     AVQRAAARLSS"
     gene            complement(9327..10124)
                     /locus_tag="PK818_04855"
     CDS             complement(9327..10124)
                     /locus_tag="PK818_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82100.1"
                     /translation="MNETVVHQPGEGPSPARRRAGTFLGRLSQALREQSWVAVMIEVG
                     IVVLGVVMGFQVTAWGAERAERAEEQALLQGLRSEFEEVLAGIDAQVAKHRRVESAVQ
                     AIVEALAQAERNGERFATIPDDTLVWALVATTTQFSQGILGGILVSGRLGLIRDPALR
                     RALAEWGGVLADVTEDEQASRELVMKDLEPTLRSRMDIRPFRRYAHHLGTLPLSEAGA
                     SSQVPVELEITGVFASRLYWLQHVIREFEEPKVEARRMLALIDRSLE"
     gene            complement(10158..10493)
                     /locus_tag="PK818_04860"
     CDS             complement(10158..10493)
                     /locus_tag="PK818_04860"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQY82101.1"
                     /translation="MAMCKRVTKQAPRMWGPSIVGYGSYTYKYESGHSGEMCLVGLAI
                     RGRELVVYLVAEDPAQVARLAKLGKHKMGKSCLYIKRLADLDAKVLEALIAGSVAEVK
                     RRYPGTGRA"
     gene            complement(10654..11280)
                     /locus_tag="PK818_04865"
     CDS             complement(10654..11280)
                     /locus_tag="PK818_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HQY82102.1"
                     /translation="MRKLVASTSVSLDGVMQAPGGAEEDPAAAFAFGGWAFPYWDEAL
                     DLTERGFEGKDREILLGRRTYEVFEGFWPQLPADNPNAQSMNGARKYVASRTRKTLQW
                     ENSVLLEGDVVEAVAALKAQPGNDLQIVGSGNLIQSLQAAMLIDEYIVWTFPVILGRG
                     KRLFEATVKPQALRLVSTKASATGVVMSTYVSAGDIAVGSFAQGGPGD"
     gene            complement(11286..11936)
                     /locus_tag="PK818_04870"
     CDS             complement(11286..11936)
                     /locus_tag="PK818_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HQY82103.1"
                     /translation="MSRLRVESFTISLDGFGAGPNQDLANPLGVGGSALHGWAMSTRT
                     FQRNVFGRDGGAAAGIDEDHAARGFRNIGAWILGRNMFGPVRGPWPDESWRGWWGENP
                     VYHLPVFVLTHHARAPLVMEGGTTFHFVTEGIAAALAQAREAAAGKDVRLGGGVNVIQ
                     QYLRQRLIDEMHIAISPVLLGAGERLLEGINLPALGYACTRHEGSPLATHVVIERQ"
     gene            complement(12582..12992)
                     /locus_tag="PK818_04875"
     CDS             complement(12582..12992)
                     /locus_tag="PK818_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HQY82104.1"
                     /translation="MAIGYSGTPLARKLGISEGSSVLLENPPEQYSEWLAPIPAKVEF
                     RARLSAKVDVVHVFADSSAALASALKRLRKGIRPSAAIWVSWPKKASKVQTDITEDRI
                     REIALPLGLVDIKVCAVNDVWSGLKLVIRKDLRE"
     assembly_gap    13086..13185
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13186..13547)
                     /locus_tag="PK818_04880"
     CDS             complement(<13186..13547)
                     /locus_tag="PK818_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82105.1"
                     /translation="MRDLATLEHPTNRAARWGARLAGALLAAFACVCSAQDATRSGPL
                     FDDLARMDEELFDTAFVKCDASKFRAIFTDDAEFYHDRTGASFGEAARTLKSCPRENG
                     VTRTLVPGSLEVYPMQGYG"
     gene            complement(13710..13985)
                     /locus_tag="PK818_04885"
     CDS             complement(13710..13985)
                     /locus_tag="PK818_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82106.1"
                     /translation="MCRRSGAIFGYYAFGTVSVQGHPENTEEYIWGDRTLKNVRCRTC
                     GIVTHWEPIDSKLGARHGVNLRNFDPRLLESVVVRRFDGADTWAYID"
     gene            complement(14291..14435)
                     /locus_tag="PK818_04890"
                     /pseudo
     CDS             complement(14291..14435)
                     /locus_tag="PK818_04890"
                     /inference="COORDINATES: protein motif:HMM:NF025742.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
BASE COUNT         2388 a         5068 c         4682 g         2197 t
ORIGIN      
        1 ccgctcagcc cgcgcgcaac gccgcgatcc gcacgtccag cggcggatgg ctggcgaaca
       61 gcgcgcccac gccgccggaa atcccgaacg ccgcgatctc cttcggcagc gcggtctgct
      121 cgtggttgcc cttgagccgc tccagcgccg cgatcatggc gtcgcgcccc tcgagcttcg
      181 ccccgccggc atcggcgcgg aactcgcgcc ggcgcgagaa ccaggccacg atcatcgagg
      241 cgaagatccc gaacacgacc tgaagaacca tcacgatcac gaagtagaag atccccggcc
      301 cgccgtcgcg gttgccgcgg atcatgccgt cgatgatgtt gccgacgatg cgcgagagga
      361 agatcacgaa ggtgttgagc acgccctgga tcagggtgag cgtgaccatg tcgccgttgg
      421 cgacgtggct gatctcgtgg cccagcaccg cctcgacctc gcgccgctcc atgccgcgca
      481 gcaagccggt gctgaccgcc accagggcct tgttgcggtt catgccggtg gcgaaggcat
      541 tgaggtccgg tgcgtcgtag atcgcgacct cgggcatgcc gatgccggcg gcctgggcct
      601 gcttctgcac cgtcgccagc agccaggcct cggcctcgtt gcgcggcttg tcgatcacgt
      661 gcgcgccggt ggcgcgcttg gccatccact tcgacagcgc cagcgagatg aaggcgccgc
      721 ccatgccgaa caccgcggcc atcaccagca ggccggcatt gcccgtgccc aggcgcaggc
      781 cgaacacgtt ctcggcgacc cacagcacgg cgccgagcag gatcatcacc gcgaagttgg
      841 tggcgatgaa gagcaataca cgcatcatgg attctggctc cttggaaacg cgggggcgtc
      901 gccggcttgc cgccgggacc gcttcgcgca ttagataggg ggcagcgcga caagttcaag
      961 cgcggcgcca cgcaaccgca aggagacccg gccttccatg ccccacccca gcgcctaccg
     1021 cggccgtttc gcgccctccc ccaccggcga gctccacttc ggctcgctgg tggcggcgct
     1081 cggcagctgg ctgcgcgcgc gcgcgcaagg cggggcctgg ctggtgcgga tcgaggacct
     1141 cgatccgccg cgcgaggtgc cgggctcggg tgaaggaatc gtgcaggcgc tggccgcatt
     1201 cggcctgcac agcgacgagg ccatcgtgca ccagggcgac cgctcgccgt cctacgccga
     1261 ggccctgcag cgcctggtcg acatcggcgc ggcgttcccg tgccactgca gccgcgccga
     1321 gctcggcgcc agcggcatcc accatgcctg cgtggcgggc ccgggcccgg cgacgcgcga
     1381 accggccatg cgcgaaccgg cttggcgcgt gcgcgccccg gcgcaggtca tcgccttcga
     1441 cgacgcgatc cagggccgct acgcgcagga tctcgccgcc gccgtcggcg attacgtggt
     1501 gcggcgcgcc gatggctggt gggcctacca acttgccgtg gtggtggacg atgcctggca
     1561 aggcatcacc gaagtcgtgc gcggcgccga cctcatcgac tcgacgccgc gccagatcca
     1621 cctgcagcgg ttgctgggct tgcccacgcc agcctacgca cacctgccgc tggcgctgga
     1681 tgcctccggc gccaagctca gcaagcgctc ggccagcctg ccggtcgacc ccggcgaccc
     1741 gctgccggcc ctgcgcgcgg cgctggcctt cctgggccag ccacttcccg cggtcccggc
     1801 cgcggccggc ccggccggcc tcctggctgc ggcagcgcgg gatttcgacc ttgcgcgcat
     1861 accgcgcaag agccgtacgc cggcgtatgc cgatgcgcaa ggcgcgccaa cggcagcacc
     1921 gcaccgctag aatcagcgga tgcggattcc gcgggccaac gacgcccgtg gagcgcagtc
     1981 actagggagg gaaaccatgg aatcacgcgt cgcactggtc accggcggaa cgggcggcat
     2041 cggctcggcc atcgtcaagc gccttgcccg catggggcat cgcgtcgcca ccaactaccg
     2101 cgatgaggcg aaggcgcgca aatggcagga gcaggcccgc gcggagggcc tggagatcct
     2161 gatcgttccg ggcgacgtcg ccaatcccga atccgccgcg gcgatggtcg atgccgtgcg
     2221 cagcgcgctg ggcccggtcg acatcctggt caacaacgcc ggcatcaccc gcgacacaac
     2281 cttccaccgc atgagcgcgc tgcagtggca ggaggtgatc aacaccaacc tcaactcctg
     2341 cttcaacgtc acccgcccgg tgatcgatgg catgcgcgag cgcaagtggg gccgcatcat
     2401 ccagatcagc tcgatcaacg gccagaaggg ccagtacggg caggccaact acgcggcgtc
     2461 caaggccggc atgcacggct tcacgatctc gctggcgcag gaaaacgcgc gcttcggcat
     2521 cacggtcaac accgtgtcgc ccggctacat cgccaccgac atggtcatgg cggtgcccga
     2581 ggaagtgcgg gccaagatcg tcgtcaacat ccccaccggc cgccttggca cgccggagga
     2641 gatcgcctac gccgtttcgt tcttcatgcc cgacgaagct gcgtggatca ccggcgccaa
     2701 cctgtcggtc aacggcggtc actacatggg ttggtagccc ttgccgcgcc cacgcgatgc
     2761 gcgcccgcgc atcgcgcgcg gcaggggcgc tgcgcaggct cgccatttct cccacgcaag
     2821 tccggcacgc accagcaccg catccgttcg gagcgagtcc ggcgacggcg gcatcggggt
     2881 gacggcgagt tggctctttc tgtctgagcc ggcaccccga tggcggcggc gccggacccc
     2941 ccacgccaat tcaaggcgcg tgcggtggcg gcggcatccg acctccgcgc cggaaacaac
     3001 ccgccacgac accaaacttc cgcagcgcaa catcgtccgc accgcagcaa cttttgcgcc
     3061 gcacaagctt ctgctagggt gccgccatgg ccaaaatccg cgtcatcaag aagtatccga
     3121 accggcgcct gtacgacacc gagatctcga gctacatcac gatcgaggac gtgcggcagc
     3181 tggtggtcga caacgaagtg ttcgaggtgc gcgacgccaa gtccaacgcg gacatcaccc
     3241 gctccgtgct gctgcagatc atctccgagc acgaggatcg cggcaccccg atgttctccg
     3301 ccacggtcct gtcgcagatc atccgtttct acggcgattc gctccagggc ttcgtgggca
     3361 gctacctcga gcgctcgctg cagaccttcc tcgagcagag ccagcaattc cgcagccagc
     3421 tcaacaacgt catcgggcag acgccctgga cgatgttgaa cgagatgacc gagcgcaacc
     3481 tcgacatgtg gaagtcgatg cagaaccgct tcctctcggc cgcgtccagc gcggtgcgcg
     3541 ccaacaacgc cccgccggaa gccaagcccg cgggcaagac cgagcgccag cgctgagccc
     3601 gtcgcgggca gcgatttgac tcgccgcccc ggcctgccta ccatccggtt cttgctgcac
     3661 cgcaccaatc ggcggtgacg gcctacattg cactgcagga acccccagcg atgactactc
     3721 gcactgccgt cgtgaccggc ggcctaggcg gactcggcac cgagatctgc aagcacctgg
     3781 cgcgcgccgg ccgcaaggtc gtggcggccg acctcgccgg gcgcgccgat cgcgtcgacg
     3841 aattccggcg cgagaccgcg gagttcaatg gcgcgatccg cttcgaggac ctcgacgtca
     3901 ctgacgaggc ctcctgcgcc gaactcgtgc gccgcgccgc cggcgactcg ggcggcgtcg
     3961 acatcctggt caacgccgcc ggcatcaccc gcgacggcac cctgcgcaag atgagttcac
     4021 agcagtggcg cgacgtgctc accgtcaacc tcgacggcgt gttccacatg acccgccagg
     4081 tggtcgacgg catgtgcgag cgcggcttcg gccgcatcgt caacatcagc tcggtcaatg
     4141 gccagaccgg ccagttcggc cagaccaact acagcgccgc caaggccggg atgcacggct
     4201 tcaccatggc gctggcgcgc gaggtcgcgc gcaagggcgt caccgtcaac agcgtgtcgc
     4261 ccggctactg ccgcaccagc ctggtgatga agatcccgga gcagatccgc aatgacatcg
     4321 tgtcctcgat cccggtcggc cgcctgggcg agccgagcga gatcgcgcgc acggtgcagt
     4381 tcctctccgc cgacgacgcc ggcttcatca ccggggccaa catcccggtg aatggcggct
     4441 acttcatgga cttctgagtt cgaccgcgca accggcccgc gaccgccctc tggcgccgcg
     4501 ggtcgccgag ggcgcaagct cgaccctggc cacagggagt tgacgatgca gcagaggcag
     4561 accaagtacg aacgcctgct cgcacgggcg cgtgaactgc ccgcgatgtc caccgcgatc
     4621 gcgcacccct gcgacgaata ctcgctggcc gcggcgctgg acgcggcccg catcggcctg
     4681 atccggccga tcctggtcgg gccggtggcg aggatccgcg ccgcggcagc caaggcgggc
     4741 ctctcgctgg acggcatcga gatcgtggac acggcccaca gccatgccgc cgccgaggag
     4801 gcggtccgcc aggtccgact gggcaaggcc gagtgcctga tgaagggcag cctgcacacc
     4861 gacgaattga tgggcgccgt gctcgaccgc gagaccggcc tgcgcacttc gcgccgcatc
     4921 agccattgct tcatcatgga cgtgccggga cacgacgacg tcatcatcat ctccgacgcg
     4981 gcgatcaaca tcgccccgga cctcgcggcg aaggtcgaca tcgtgcaaaa cgccatcgac
     5041 ctcgcccatg ccctcggcgc gggcgaggtc cgtgtcgcca tcctctcggc gatggagacc
     5101 gtcaatccca aggtgccgtc gacgatcgag gctgccgcgc tgtgcaagat ggccgatcgc
     5161 ggccagatca ccggggccat cctcgacggc ccgctcgcgc tcgacaacgc catcgacccc
     5221 gaggccgcgc gcatgaagca gatcgtgtcg ccggtggccg gtcgcgccaa cgtgctgatc
     5281 gtgcccgacc tcgaggccgg caacatgctg gccaagagcc tgaccttcct cgccaatgcc
     5341 gatgccgccg gcatcgtgct gggcgcgcgc gtgccgatca tgctcaccag ccgcgccgac
     5401 tcggtggaaa cccgcctcgc ctcctgcgcc gtggcctcgc tggtcgcgaa ggcccggcgc
     5461 gacgcgctgt cgcgcaaggt ggtctgagcg cccgcaatca gcccggcgtc ccgcgcgggc
     5521 aggagatcca tggccgacgc catcgcagtg ctcaatgccg gttcctcgag catcaagttc
     5581 tcggtgtacc tgcacgatgc cggacgcctc gaagtcgccc tgcgcggcca ggtcgagggc
     5641 ctgcacacgg cgccccgctt cgtggccagg gaccggtccg gccaggtcga cgccgagaag
     5701 tcctggggcg agggcgtgtc cctgggccat gacggcgcga tggcccacct ggtcggctac
     5761 ctgcgccagc gcctgggcgg cagccggctc ctcggcatcg gccatcgggt cgtgcacggc
     5821 ggcatgcatt tctcgcagcc cgtgcgcgtg gatgcgcaag tcctcgaaat gctccggcag
     5881 ttcgtgccgc tggcgccatt gcaccagccg cacaacctcg cgccgatcgg gcagatgctc
     5941 gaaagcgcgc cggagctgcc ccaggtggcc tgcttcgaca ccgcgttcca ccgcgacaat
     6001 cccgaattgg cgcagatgtt cgcgctgccg gcggaactgc acgcgcaagg cgtgcgccgc
     6061 tatggcttcc acgggctttc ctacgaatac atcgcctcgg tcctgccggg catcgatgcc
     6121 cgcgccgcca gcggccgcac ggtcgtgctg cacctgggca atggcgccag catgtgcgcg
     6181 ctcgaaggcg cccgcagcgt ggccagcacg atgggcttca ccgcggtcga cggactgctc
     6241 atgggcacgc gctgcggcgc cctcgatccg ggcgtgatcc tgtacctcat ggaccagcgc
     6301 ggcatggatg cacgcgcgat cgagcagctc atctactcga aatccgggct gctcggcgtt
     6361 tccggcatct cgagcgacat gcgcaccctg ctcgccagcg acgacccccg cgcacggctg
     6421 gcggtggaac tgtatgcgta ccgcatccgg cgcgaacttg gctcgctcgc cgcggcgctg
     6481 ggtggcctcg acgcgctggt cttcaccgcc ggcatcggcg agcatgccgc gcccatccgc
     6541 gagcgcgtgt gccgcgacgc ggcctggctg ggcctcgtgt tcgatgccgc cgccaacgcc
     6601 gcgcacggcc cgcgcatcag cagcgcggcc agcagggttt cggcatgggt ggttcccacc
     6661 aacgaggaac tgatgatcgc gcgccatacg ctggccctgc tggcggcctg accatcggcg
     6721 cgagcggctg cgtcgatgcg cccacggccg cgcacggcgc ccggcccggc gcgatgacga
     6781 tcaagctcgc ccgatggtag tcacgcgcgc gaacgctgcc gcaggtggca agtcagagcg
     6841 agcgcccgtc gtattcctgc acggggatcg tcgaaagctc gatgtcatcg aggcagcgga
     6901 tgttgatcgc ggccatcgcg ttgcccttcg gatcggtgcc ttcgccatag ggatggatac
     6961 cgcacaccgg gcagaagcga tgcttgatga cgtggcggtt gaaggtgtat gtgctcgccg
     7021 cgccctcggg cgtgagcaac tgcagctgcg cgcgcggcac gaaccagagc agtgaaccct
     7081 tgcgctggca gatcgagcaa ttgcatgcca tcgcaccctt gatctcgcct tcgacctcga
     7141 aggcgacccg gccgcagtgg cagctaccct tgtgcttcat gtgcccctcc tggatctgtg
     7201 cggcctggcc ggcagcggct gtcgcaggga actaggcatt ggcccccggc ccgccagcgg
     7261 tccccttccg caagagcgcg ccaattcgga tgcccagcca tgccatcggc aggtacgcga
     7321 ccagcaggtc cagcgcgatg aaccaggccg gcgcgggaat catgaaactg gccgccacgc
     7381 ccccgcacag gaagaccgcg ccgatcacgt aggccagttg cggcctgaag ctgttcgcga
     7441 acaggtaggt caccagagcg cccgcaagcg tgcccagggc gtgcgccagg aacggcatga
     7501 tgaaatgccg cggctcgaac aggtgcatcg aggccgccag gctttcggcg ttggtcacgt
     7561 ccacgcccgc gggaggcggg atcagcgacg gactgatggt gatgagcgcc atgttgacgc
     7621 tgccgccgag aaccaggccg gcaaggacgg cgagcgtgtt tcgcagcagc ttgggcatgt
     7681 gcacttcccc tcgaatgcct gttgccgacg ctcaatcgcg gccacgccgt cggggcgggg
     7741 ttccacaggc gaggacgtcg gcccgaatac gatgccagcc ggcgcccggc ccgcgcaaca
     7801 acccggctgg aagggccgcg gaaaccgccc gttcgccggc cccctcctcg cagcgacgcg
     7861 cagcgggccc ctggacccgg gctccggccc gcgagaggcg caaggcccta ctggcgcacg
     7921 aacaccatga cccagttcgt ttcccaggtc gtgccgccgt cgatcgaaaa cgcctgctcc
     7981 catcgcgggt ttccaccggg gttcacggtc cagatgaagc ggacgtccag cggccggcca
     8041 tcgagactgt ccttggcatg gaagacgccg acgggtccat cgaatcttcc aacgaccggc
     8101 acgtcgagat ggtgcggggc acgaccatcg agccaccaga tcgcccactc ctgcgatgcg
     8161 tcgttgtacg accggaatgc cgcggcccgg acctctccgt caggaaaacg caggacgttg
     8221 tcctcgacat tgccaaagcc accaaggatc ttccgggtcg aactcaggcc gggaaactcg
     8281 gtccagtcgg tgcacccgga caggcgcgaa tcgaggcggc gatgctggac gtgccagtcg
     8341 ccgatgagga agtcgaagtc ggacggcgcg cgcctggggt tcgagatgga catgcagttc
     8401 aggatccggt gccgcgagca gtcttgcgcg ctcatgatcc catgcgtgaa ctcaggatga
     8461 caggcgcgcg gcagcccgct ggacggcggc atcgcgggca ctgcgcacct cctcataccg
     8521 cgcttgcgca gtggccagga cctggacggg cgccagttcg ggcctcccgg cattgaacgg
     8581 cgggtcgggt gcgtattcga tccgcagctg caccgactgg gcgaatgccg cgcccgcgat
     8641 ctcggcgagc acggtcaggg caaagtcgat tccggcggtg acgccaccgc ccgtgaacac
     8701 gttgccgtcg cgaaccaccc gtgcggagtc cggctttgcc ccgaacgccg gcaaggcctc
     8761 gcgccaggcc cagtgggtcg tggcgcgctt gcccttcagc aatcccgccg cgcccagcac
     8821 cagcgcgccg gtgcagaccg aggtgacgta gcgcgcggtc ccggcaaggc gccggacctg
     8881 cgcgagcagt tcctggtctt ccatcgctgc aatcgtgccg aagccgccgg gtacgcagag
     8941 cagcgcgcag cgctcgacat ccgcgagccg ctgcaccctg gcgaacacca gtcccccgtc
     9001 ggcctcgaga tcgccgccgg tgctcgaggc caggatgcat cgggcgccgg gaagtcgcga
     9061 gaaaacctcg tggggcccgg tgaagtcgag ctgggtgacg tgcgggtaga ccgcaaagac
     9121 gaccggaaac tcctcagcca cggtgacctc ccttgcctac gatggtatgg acgcgaggcg
     9181 aacaatgcct gcggcacccg cggcgtcacg ccatgtgctc gatcgccgcg gggaggccga
     9241 gaccgacggc gatcggtgtc ggctctaagg ccacggttgc acggggttgc ccgcatgggc
     9301 aggcagcgat ccggctagcc atgggctcac tccagcgatc gatcgatcag cgcaagcatc
     9361 cgccgggcct cgaccttcgg ttcctcgaac tcgcggatca cgtgctggag ccagtagagc
     9421 cgcgaggcga acacgccagt gatctcgagt tccaccggca cctggctcga agccccggcc
     9481 tcggacagcg gcaatgtgcc cagatgatgc gcgtaccgcc ggaacggtcg gatgtccatc
     9541 cggctcctga gggtcggctc caggtccttc atcacgagtt cgcgcgaggc ctgctcgtcc
     9601 tccgtcacgt cggcgagcac gccgccccat tccgccagcg ccctgcgcaa tgccgggtcg
     9661 cggatgagcc caagccgccc gctcacgagg atcccgccca ggatgccctg gctgaactgc
     9721 gtggtcgtgg caaccagggc ccacaccagc gtgtcgtccg ggatggtcgc gaaccgttcc
     9781 ccattgcgct ccgcttgcgc cagcgcctcg acgatcgcct gcaccgctga ctcgacgcgc
     9841 cggtgcttcg cgacctgcgc gtcgatcccg gcaaggacct cctcgaactc ggaccgcagg
     9901 ccctggagca gcgcctgctc ctctgcccgc tctgcccgct cggcgcccca cgcggtgacc
     9961 tggaagccca tgacgacgcc gagcacgacg atgccgactt cgatcatgac cgccacccag
    10021 ctttgctcgc gcagcgcctg cgacaagcgg cccaggaacg tgcccgccct ccgtcgcgct
    10081 ggagacgggc cctcacctgg ctgatggacg acggtctcgt tcatgtcggg tatcgaagcg
    10141 agtggacgag cgagtgctca ggcgcggccg gtgcccggat agcggcgctt cacttccgcg
    10201 accgatccgg caatgagtgc ctcgagcacc ttggcatcga ggtccgccag gcgcttgatg
    10261 tacaggcagg acttgcccat cttgtgtttc ccgagcttgg ccaggcgcgc gacttgcgcg
    10321 gggtcctccg cgaccaggta gacgaccagt tccctgccgc ggatcgcgag gcccacgaga
    10381 cacatctcgc ccgagtggcc gctctcgtac ttgtaggtgt agctgccata acccacgatg
    10441 ctcgggcccc acatcctggg cgcctgtttc gtgacccgct tgcacatggc catgatggcc
    10501 ttgcaatccg ccagctgccc gggactggcc ctggatgcca ggtatgcatc gatgctggcc
    10561 cccgtgggcc tggtcttggt cgctgccatc ggtggctcct gtgaagcgga cagcacgatc
    10621 ctcgccggtc aacctggcac cgcgatcgaa tgcctaatcg ccgggcccgc cctgggcaaa
    10681 tgaaccgacg gcgatgtcgc cggccgacac gtaggtgctc atgaccacac cggtggccga
    10741 ggccttcgtg ctgaccaggc gcaacgcctg cggcttgacg gtcgcctcga acagccgctt
    10801 gccgcgcccg aggatgaccg gaaaggtcca gacgatgtac tcgtcgatca gcattgcagc
    10861 ctgcagcgac tggatcaggt tgccgctgcc gacgatctgc aggtcgttgc ctggctgcgc
    10921 cttgagtgct gccaccgcct cgaccacatc cccctcgagc aggaccgagt tctcccattg
    10981 cagtgtcttg cgcgtgcgcg acgccacgta cttcctcgcg ccattcatcg actgcgcgtt
    11041 gggattgtct gccggcagct gcggccagaa cccctcgaag acctcatagg tccggcggcc
    11101 aagcaggatc tcgcggtcct tgccttcgaa gccccgttcg gtgagatcca gggcctcatc
    11161 ccaataggga aacgcccagc cgccaaaggc aaaagcggcc gcgggatctt cctctgcccc
    11221 cccgggggcc tgcatcacgc cgtcaagcga aacggatgtg gaggcaacca gctttctcat
    11281 cgtggtcact gccgttcaat gaccacgtga gtggccagtg gcgagccttc gtgccgggtg
    11341 catgcatagc ccagggcggg aaggttgatt ccttcaagga gtctttcacc agcgccgagc
    11401 aggaccggcg aaatggcgat atgcatctca tcgatcaggc gctggcgcag gtactgttga
    11461 atgacgttca cgccgccacc caggcgaaca tccttgcccg cggccgcctc gcgcgcttgc
    11521 gccagggcag cggcaatgcc ctcggtgacg aagtggaagg tcgttccccc ttccatgacc
    11581 agcggtgcgc gggcatggtg ggtcagcacg aacaccggga ggtggtagac cgggttctcg
    11641 ccccaccaac cccgccagct ctcgtctggc cagggtccgc gaaccggccc gaacatgttc
    11701 ctgccgagga tccaggcccc gatgttccgg aagcctcggg cggcatggtc ctcgtcgatg
    11761 cccgctgctg ccccgccatc gcgaccgaac acgttcctct ggaaggttcg cgtggacatg
    11821 gcccagccat gcagcgcgct gccacccacc ccgagcgggt tggcgaggtc ctggttcggg
    11881 ccagcgccga agccgtccag tgagatggtg aaactttcta cccggagcct ggacatggtg
    11941 ctactccggg tgcgagtgac caggaaccgt gggcttcagc gccaacgtcc gtgcaacgcc
    12001 gccgccgcgc gaggcgcgac aggaaggcaa ggaccccatg ctcggcggac ggcttgaatg
    12061 catcgtcagg gctcatcgtc cggctcgcct gcaaacgatc gcgcgcctgg cggtacaagc
    12121 acccctgcaa gcttgctgga ggtccagagg tgaacggcac tacccaggtc aagtgtctga
    12181 tccaggctac cacccttgat gttgaccgtg ccgggatgac cactgctctg gtgccacagg
    12241 cgcgagccgc aaccaggaca gaatgcgcag tccagcgtgt gcttcgacgc agtcgggcgc
    12301 gaccaccacg tcggggttcc cttcagtacc cgtagagcgc tccttgccac ggtgaaggag
    12361 atgccgaacg cggacgacga ctgcttccgg cactccgcgc agtggcatac gtagagcact
    12421 agaggcgctt tcggcaccgc aaagcgcacg gcgccgcatt ggcagccgcc cgattgctcc
    12481 ccgggacctg gcgacaagga ttcagccatg agccctcacg ttcgagctag ccggcccgcg
    12541 gcgattgccg cagcgggtcc cgttgggcga gggtccaggc ctcattcgcg caagtccttg
    12601 cggatgacga gtttcaaccc cgaccagaca tcgttgaccg cacagacctt gatgtccacc
    12661 aggccgagcg ggagcgcgat ctcgcggatc cggtcttcgg tgatgtcggt ctggaccttc
    12721 gacgccttct tgggccagga cacccagatc gcggcacttg ggcgaatgcc ctttcgcagg
    12781 cgcttcaggg cgctggccag tgccgcgctg gagtccgcga agacatggac cacgtctact
    12841 ttcgcggata gtcgagccct gaactcgacc ttcgccggaa tcggagccag ccactccgag
    12901 tactgctccg ggggattctc caggagcacg cttgaacctt cggagatgcc gagcttcctg
    12961 gccaggggcg tgccggagta gccaattgcc attttcatga ggcccaacgc tcgtgttagc
    13021 cggctcgcgg aggcaagtgc cgtgggcccg agccgagatg atgcaccatg tcccacagct
    13081 cgggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgta gccctgcatg
    13201 gggtagacct cgaggcttcc aggtaccagg gttcgagtga ccccgttttc gcggggacac
    13261 gacttgaggg ttcgcgccgc ctcgccgaac gatgcgcctg tacgatcgtg atagaactcg
    13321 gcgtcatcgg tgaagatcgc ccggaacttg ctcgcatcac acttgacgaa ggccgtatcg
    13381 aagagttcct cgtccatgcg ggcgaggtca tcgaagagcg ggccggagcg ggtcgcatcc
    13441 tgtgccgaac aaacgcaggc aaacgcggcc aggagtgcgc ccgcgagccg ggctccccac
    13501 cgggccgcgc ggttcgtcgg gtgttccagc gtggcgagat cgcgcatggt tgtcctttgt
    13561 ggtgcctgac attcgacttg accggcccat ctcggcaagc gccgcaaggc ctggccggga
    13621 cgacgcatca tgtcgatcgc aacggcctgg ctacgcttgc cgtggcagat ccgtttgaac
    13681 gaacgcttgc acggcacggt ctcagctgct caatcgatgt acgcccaggt gtccgcgcca
    13741 tcgaagcgcc tgaccaccac ggactccagc agcctgggat cgaagttgcg caggttcacg
    13801 ccatgccgcg ccccgagctt cgaatcgatt ggctcccagt gcgtgacgat gccgcaggtc
    13861 ctgcagcgca cgttcttgag cgttcgatcg ccccagatgt actcctcggt gttctccgga
    13921 tggccctgga cggagaccgt tccgaacgcg taatagccga agatcgcgcc cgagcggcga
    13981 cacaaggaac agttgcagct cgtcgcctgc tctggcgcac tcggaagcgt gaggcgaatg
    14041 gccccgcaat ggcaggaccc ttgaagcgaa atcccgggat gttgctgaga catggattga
    14101 gcagtcgatg gttgtcatgc cgcctggcgt tcgagccggg tcgcccgcag cggcaaggac
    14161 cgctggagcg acgatagacc agtggcgctg gaactgcaca tgccgctgcg gctcgccgcg
    14221 accgggaggt caggcaccac gcgcgaccta ggaaggcgag ctgcagccaa ggcgccttct
    14281 tggcgagtac ttccgccatg cgggtctgcc acccggcacc gcttgctctc cagcggacca
    14341 gggcatccgc tggaaggcga aggctgacgc ctgggtcgcc tgtccaagcg tggcgacgcc
    14401 tacctgcgca tgctgctcgt ccacggcgcc cgcgc
//
LOCUS       DAORUK010000048        14432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_4030_length_14432_cov_22.274675, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000048 DAORUK010000000
VERSION     DAORUK010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 14432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14432
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4030_length_14432_cov_22.274675"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..935)
                     /gene="queF"
                     /locus_tag="PK818_04895"
     CDS             complement(123..935)
                     /gene="queF"
                     /locus_tag="PK818_04895"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162143.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HQY82107.1"
                     /translation="MSATEHSPLGRDVAYPRRFDAGLLYPIPRALGRAAIGVGAGALP
                     FAGADRWQAYELSWLDARGKPRVATATFEVPAGSPNLVESKSLKLYLNSCNSARFADP
                     EALRMQVETDLSAAAGAPVAMAFGLAPVRASAGDSIDALELDIDRYGPPDAALLAADP
                     AVIVGETLSSALLKSNCPVTGQPDWADLEIAYHGPRIDRAGLLRYLASFRDHAEFHEQ
                     CVERIFGDLMQHCRPQRLSVHARYTRRGGLDINPWRTTPGMPAPAPGRSERQ"
     gene            complement(949..1401)
                     /locus_tag="PK818_04900"
     CDS             complement(949..1401)
                     /locus_tag="PK818_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82108.1"
                     /translation="MNTSLRVLATALAVLMAAGCNRQGPEVPRAVDDNKSVDDLGDGV
                     QSVVVNPDAAKVGSAVDGQGAIATAASEFTDGQTIYISVPTKFGRKQGERLEAFWFHD
                     DGRSRKDDRKVIAGPFTVFEMIAKDAGPYNVEIDANGRPIALVQFTIK"
     gene            complement(1533..2633)
                     /gene="zapE"
                     /locus_tag="PK818_04905"
     CDS             complement(1533..2633)
                     /gene="zapE"
                     /locus_tag="PK818_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HQY82109.1"
                     /translation="MTAAPLPSRRYADGVARGDWLHDPAQQQALRELDRIHAALLEPV
                     AGPGWLDRLFGAQAPPAPAGLYLWGGVGRGKTFLIDLFFDGLPLREKRRTHFHRFMRE
                     VHERLREHAGERDPLAAIARQWRASLRVLVLDEFFVSDIGDAMLLGRLLERLFAEGVV
                     LVTSSNAAPPELYKDGLQRARFLPAIALLERQCAVVHLDGAHDYRLRALTCSPVYRAP
                     LDAQSDRWLSERWHALGGDDAHVDAGIDIDGRRIAVRARCKGMCWFDFAALCEGPRGT
                     ADYIEVAREFHTVLLGGIPRLDAGNDDAARRFVHLVDELYDRHVNLVCSADAAPAALY
                     AGERLAAAFARTASRLVEMQSTDYLALEHRGG"
     gene            complement(2630..3304)
                     /locus_tag="PK818_04910"
     CDS             complement(2630..3304)
                     /locus_tag="PK818_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQY82110.1"
                     /translation="MSERPAQAAADPALSIAGPAGRIEAALDLPEADAVPRPLLAIVC
                     HPLPTEGGSMHNKVVTMAARALRELGATTLRFNFRGVGHSEGRFDDGAGELDDLRAVA
                     AWARAQHPGKALWLAGFSFGAWVSLRAAAELGADAVVSIAPPVGRSWDFAGIVLPAAR
                     WLVIQGDADEIVEPGAVHAWVAALPHPPQLVRMPETSHFFHRKLMDLRGALKHGVRDW
                     LPAPAA"
     gene            complement(3389..3904)
                     /locus_tag="PK818_04915"
     CDS             complement(3389..3904)
                     /locus_tag="PK818_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQY82111.1"
                     /translation="MRNYDLEFLKRFSMVIAFLMAVTVGLILFARHLNATVQREPDPA
                     AQQRVLERIAPAGAVYAGATGAAQQAAALEAATAAAAAEVAYGGTTDGSVIYNNLCTG
                     CHTSGAGGAPMLDAAGIGARVAKGVDTLVTNAIQGYTGDAGVMPAKGGNPALTDEQVK
                     ATVEWMVAQAK"
     gene            4347..10349
                     /locus_tag="PK818_04920"
     CDS             4347..10349
                     /locus_tag="PK818_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82112.1"
                     /translation="MKHSDGSASTRRARRIKLAPMTRAVRMALAASVAALALGAATPA
                     FAAGPKVPLELQLSQGAIPSERLPVIDLTTVPMFSTAEVPVELHPTAISQYSPGDVSI
                     YNDAPISESDVFYDMAAISGYSDYGAVEITNTANGELYAGSLFGDAIGIYGYSLTGDV
                     SIDNAGDITAYSYAGLADGIFASGANVEVANSGAIESYGYTWSAGIEAQGADSVVVDN
                     SGDITAYTGPFVQVVDGYGYTIGYSAGGTAYGIYATGGEAGASVTNSGNIEVQGGYVT
                     GIEVQSSGDLVVDNSGGIVAGSGLNSYYNGSNYYTYYFGTQVATGINATSNGEGAAVS
                     VSNSGDISADGIFGATGIAATASGYGGSASVTNSGAIYASQNQKYGYGAYGIVVSSDG
                     DGSIDNSGSIEVYSGGVANGATVLSFAGDASVTNSGDISVYSAAALYYAATGITAFAA
                     NGSAHVDNSGSVDASAKYWATGVNAQAYGDVTVDNSGSLYADGQKYAFGVYAASNAGD
                     VVVHNEDGGEIGFYSYLGRGWGVFAYAGQGDVHVDNGGTIAGYAYGQSAGVFGLAGAG
                     NVSVDNSGTIDVVSGGGTAVGVFARADYGTASVSNSGDITASVGSYYYSGFAAYGVLA
                     RGAYAEVSNSGNIVAEGYYYATGIAATSYYGTLVHNTGGSISAYALGGATGIDALSLF
                     GDVVVDNASDIEAVGLVFGAQGLRAYSVYGDVEIHNTGDIYAGSLYGDAIGIYGYSLT
                     GDVSIDNAGDITAYSYAGLADGIFASGANVEVANSGAIESYGYTWSAGIEAQGADSVV
                     VDNSGDITAYTGPFVQVVDGYGYTIGYSAGGTAYGIYATGGEAGASVTNSGNIEVQGG
                     YVTGIEVQSSGDLVVDNSGGIVAGSGLNSYYNGSNYYTYYFGTQVATGINATSNGEGA
                     AVSVSNSGDISADGIFGATGIAATASGYGGSASVTNSGAIYASQNQKYGYGAYGIVVS
                     SDGDGSIDNSGSIEVYSGGVANGATVLSFAGDASVTNSGDISVYSAAALYYAATGITA
                     FAANGSAYVGNSGSVSVTSAAILGAATRAVDAQGQQGVVVVNSGSLYANGKYAYGVYA
                     LSTAGDVAVHNEDGGEIGFYSYSGTGFGVLGISYLGDVDVDNAGSIEGYAYGQSVGVF
                     GFSGDGDVSVHNSGSISAVTGGNAAVGVFARADYGTASVSNSGDIYAGVNGVNGYIGD
                     VAYGVLARGEYADVGNSGSIEAVGFYYATGIAARSYYGTTIATSASSQIEATALLVAT
                     GIEGRVEFGNVVVANAGDIMVEGVYGGGVGIQAYAYFGSVSVANAGDILAGSTYGTAI
                     GVTGAVVFGNTVVSNSGTIEAAGYYGAYGIAAQSYFGDVTVSNSSSGHVHAAAPGYAY
                     GVFASAYGDVTINNAGDVSASADDGGSAAAVLMESLYGHSTLNNAATGSISVEGTDGY
                     AFAVVGSDAVDTINNSGHIYGAISLLGGNDAFNNRAGGLWDVDGTVSTDFGDGNDTLG
                     NAGSVRIDDGGAISFGAGDDSINNLAGGVIRLAGGSIYMGAGNDGILNLGTIAVAGDD
                     NLIDLGGGAQDVLTNVNTINFLDGHTDDALAIHGNLGGTGSLGLDVDLADESSDQLYV
                     EGSMVSNAAQRVNIAFAGMPLVASTSIDFAHVSGTSAAGSFVPGQILGYNYARNFLDL
                     AFTIGSRINTANTADDVFSIELDVVGLNDTGTLAANVASGAAGMLNAQVGTFKQRMGV
                     NPYGDDGKVMSAFFRTYTSEGDVDPAHMAANFGQGGNFAFDQTTWGREVGVNANLHGN
                     FHAGVTLGSADGRQRLTGNGVGENRMDGMTWGWYATWFAPQGFYVDLSGRWMAVDVRS
                     TSAAGQLESRAHTGAWNLEAGYQWTLAGLSVVPQLQYTRTEVEDVRTIYGDRANFEGH
                     GGTSSRGRLGVEISKTFESGDMRWTPYGSLNAIREFDGEMSYTVADNFFGSTDTEGTS
                     VMAELGLGVQKGGWGFTIGANWADGGAFQSTVSGQALVRFAW"
     gene            10415..11620
                     /locus_tag="PK818_04925"
     CDS             10415..11620
                     /locus_tag="PK818_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03032 family protein"
                     /protein_id="HQY82113.1"
                     /translation="MSTEKTPDAGPTQPGNATPELETGLDTVGIEPPAAAPAAANAGK
                     PPRDLTKLKVEKGCSRGLAGWLGQHRLSLAITSYQSGRIYLVGSDKQGRVSFFERIFE
                     RAMGVVGNAQRIYLGGLYQLWRFENVLRPNEAIHGQFDKCYVPRNAQTIGDLDIHELG
                     IQANGKVIFVNTKYSCLAELSQTHSFKAVWKPKFISKLAPEDRCHLNGLAMVEGRAKY
                     VTAVCRSDSVDGWRDRRQDGGVVIDVETDEIVCEGLSMPHSPRWHNGRLWLLNAGTGH
                     LGWVDFEKKAFVPHTFVPGFARGLSIIGNVAAVGLSKPRNQRFEGLQLDEELKKRDAE
                     PWCGVQIVSLANGDVMNWIRFEGDITEIFDISFLPNVKNPMMIGLRTPEIRDLITFES
                     ELAVEAPAA"
     gene            11617..12048
                     /locus_tag="PK818_04930"
     CDS             11617..12048
                     /locus_tag="PK818_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HQY82114.1"
                     /translation="MNRATFLLAAMIALCGPQAWAQAAKPAPQPAAADPAAEAFKAWD
                     KDKNGNLSLVEFRAGWQQVQRVAETQARLRQQFAAVDTNRNGAIDPGEYGSLVLVKQA
                     GKNAPQMSTFDGNRDGKLGFGEYVKLVQALAPKEPAKAAAK"
     gene            12045..12641
                     /locus_tag="PK818_04935"
     CDS             12045..12641
                     /locus_tag="PK818_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82115.1"
                     /translation="MKVDIVPCWKQVRPELAQELMAFWRDNKAIGDDAAAAARAMQAV
                     CVARDESGALCGVGTAVVKVLPRLRQPMYYYRQYFAKGLRGQHQELPFYLRAKQVLQD
                     YNASLDRPESLGILLEVENSKIAAAYKHAYEPAFDATFIGYSPRGLQLRVSYFDGAEL
                     LAPAPAPAPVRGTAMAARATIPDPTRIQPASRRGNARS"
     gene            12674..14293
                     /locus_tag="PK818_04940"
     CDS             12674..14293
                     /locus_tag="PK818_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007967835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HQY82116.1"
                     /translation="MSLLSLAVVSGLAMSAPAEAAGNQKSAAIARANALIAGPAAASV
                     RRAGADGFVARDVVVDADGTEHVRFERTYQGLPVIGGDFVLQSRNGKVGKVSQTLKTS
                     ARPGLAGRIGADQAIVEAGARFGSTFIGAPTSRKVVYALGASPVLAHEVVFNGVKADQ
                     TPTVMHYIVDAANGKVLNQWDEIHTARPGPGGSSCAGTAAAATGASLMVGSVALTTTK
                     CSATSYEMRDPTRGGGYVTNMAMKTSGNGTVFTDSDNAWGNGLTSNSQTVAVDAQFGI
                     AKTWDYFLTVHGRNGIAGDGRGSFSRVHYGRNYANAFWSDSCFCMTFGDGDNGASIYP
                     LVSIDVAGHEMSHGVTSRTANLIYNGESGSLNEATSDIFGTMVEYYANTAADPADYVM
                     GEELYPNNANMSNAIRWMFKPTLDNYSQHCYDPATTPTFEVHGGSGIANSFFYLLAEG
                     AVVPAGFGAGSWANLSASSLVCNGNTALTGIGRDAAGKIWYRALTVYMTSGTNYAGAR
                     AATISAATDLYGGTSAQTNAVKAAWSAVNVN"
BASE COUNT         2381 a         4961 c         5013 g         2077 t
ORIGIN      
        1 agacggccat tgtcgggctt cgccgcggat ccggcaaagg cgggatgccg gtgcggggcg
       61 cgatgggcgg gcattgccac ccgctgccgc gcatggggtg ccgcgcttgc cggtgcgcgg
      121 cgctactggc gttcgctgcg cccgggcgcc ggcgccggca tgcccggggt ggtgcgccac
      181 gggttgatgt cgaggccgcc gcggcgggta tagcgggcgt gcacggacag ccgctgcggc
      241 cggcagtgct gcatgaggtc gccgaagatg cgctccacgc attgctcgtg gaactcggcg
      301 tggtcgcgga agctggccag gtagcgcagc aggccggcgc ggtcgatccg cgggccgtgg
      361 taggcgatct ccaggtccgc ccagtccggt tgcccggtga ccgggcagtt ggacttcagc
      421 agcgcgctgg acagggtttc gccgacgatc accgccgggt ccgccgccag caatgctgca
      481 tccggcggcc cgtagcgatc gatgtcgagt tcgagcgcat cgatgctgtc gcccgcgctg
      541 gcgcgcaccg gcgccagccc gaaggccatc gccaccggcg cgccggcggc cgccgagagg
      601 tcggtttcga cctgcatgcg cagcgcctcg gggtcggcga aacgcgcgct gttgcaggaa
      661 ttcaggtaca gcttgagcga cttggattcg accaggttcg gcgatcccgc cggcacctcg
      721 aaggtcgcgg tggcgacccg cggcttgcca cgcgcatcca gccacgacag ttcataggcc
      781 tgccagcgat cggcgccggc gaacggcagc gcgccggcgc cgacaccgat cgccgcgcgg
      841 cccagcgcgc gcgggatcgg gtacagcagg ccggcatcga agcgtcgtgg gtaggcgacg
      901 tcgcggccca gcggagagtg ttcggtcgcg ctcatcgccg ccggcgggtc acttgatggt
      961 gaactgcacc agcgcgatcg gacggccgtt ggcgtcgatc tcgacgttgt acggcccggc
     1021 gtccttggcg atcatctcga acaccgtgaa cgggccggcg atcaccttgc ggtcgtcctt
     1081 gcgcgagcgg ccgtcgtcat ggaaccagaa tgcctccagg cgctcgccct gcttgcggcc
     1141 gaacttggtc ggcaccgaga tgtagatggt ctgcccatcg gtgaactcgc tcgccgcggt
     1201 ggcgatcgcg ccctgcccgt cgaccgcgga gccgaccttg gccgcatccg ggttgaccac
     1261 caccgactgc acgccgtcgc cgaggtcgtc cacggacttg ttgtcgtcca ccgcgcgcgg
     1321 gacttccggg ccctggcggt tgcagcccgc cgccatcagc accgccaggg cggtcgccag
     1381 cactcgcaaa ctcgtgttca tcgccatcct ctcgcggaat cggggccctg cagcgcagcg
     1441 ggcacggccg cgcattctag tgcaggcccg gcgccgccgc cggcgcgcgg acagtcatcg
     1501 ttcagcgcac gcgcagccgc gctcagccgc gctcagccgc cgcggtgctc cagcgcgagg
     1561 tagtcggtcg actgcatctc gaccagccgc gaagcggtgc gcgcgaacgc cgcggccagg
     1621 cgctcgccgg cgtacagcgc ggccggcgcg gcgtcggcgc tgcacaccag gttgacgtgg
     1681 cggtcgtaca gttcgtcgac caggtgcacg aagcggcgcg cggcgtcgtc gttgccggca
     1741 tccaggcgcg ggatcccgcc gagcagcacg gtgtggaact cgcgcgcgac ctcgatgtag
     1801 tcggcggtgc cgcgcggacc ctcgcacagg gccgcgaaat cgaaccagca catgcccttg
     1861 cagcgcgcgc gcaccgcgat ccggcggccg tcgatgtcga tgcctgcatc gacgtgggcg
     1921 tcgtcgccgc ccagcgcatg ccagcgttcg gacagccagc gatcggactg cgcgtccagc
     1981 ggcgcgcggt acaccggcga gcaggtgagc gcgcgcaggc ggtagtcatg cgcgccgtcg
     2041 aggtggacca ccgcgcactg gcgctccagc agcgcgatcg cgggcaggaa ccgcgcgcgc
     2101 tgcaggccgt ccttgtacag ctccggtggc gcggcattgg aactggtcac cagcaccacg
     2161 ccctcggcga acaggcgctc cagcaggcgc ccgagcagca tcgcgtcgcc gatgtcgctg
     2221 acgaagaatt cgtccagcac cagcacccgc agcgaggcgc gccactgcct ggcgatcgcg
     2281 gccagcgggt cgcgctcgcc ggcgtgttcg cgcaggcgtt cgtggacctc gcgcatgaac
     2341 cggtggaagt gggtgcggcg cttctcgcgc agcggcaggc cgtcgaagaa caggtcgatc
     2401 aggaaggtct tgccgcggcc gaccccgccc cacaggtaca ggccggcggg cgccggcggc
     2461 gcctgcgcgc cgaacaggcg gtccaaccag cccgggccgg ccacgggttc cagcagggcg
     2521 gcgtggatgc ggtcgagctc gcgcagggcc tgttgctgcg ccgggtcgtg cagccagtcg
     2581 ccgcgggcca cgccgtccgc gtagcgccgc gagggcagcg gtgccgcggt catgcagccg
     2641 gcgccggcag ccagtcgcgc acgccgtgct tcagcgcgcc gcgcaggtcc atcagcttgc
     2701 ggtggaagaa gtggctggtc tcgggcatcc gcaccagttg cggcggatgc ggcagcgccg
     2761 cgacccaggc atggaccgcg cccggctcga cgatttcgtc ggcatcgccc tggatcacca
     2821 gccagcgggc ggcgggcagg acgatgccgg cgaagtccca gctgcggccg accggcggcg
     2881 cgatcgagac caccgcatcc gcacccagtt ccgccgcggc ccgcagcgag acccaggcgc
     2941 cgaagctgaa gccggccagc cacagggcct tgcccgggtg ttgcgcgcgc gcccaggcgg
     3001 cgaccgcgcg gaggtcgtcg agttcgccgg cgccatcgtc gaagcggccc tcggagtggc
     3061 cgaccccgcg gaagttgaag cgcagggtgg tggcgccgag ttcgcgcagg gcgcgcgcgg
     3121 ccatcgtcac caccttgttg tgcatgctgc cgccctcggt cggtagcgga tggcacacga
     3181 tcgccagaag cgggcgcggc acggcgtcgg cctcgggcag gtcgagcgcg gcttcgatcc
     3241 ggcccgcggg ccccgcgatg gacagggcgg ggtcggcggc ggcttgggcg ggtcgttcgc
     3301 tcatgcgtgc atcatgccgc agccggtgtt gcgcctgcgc aaatgcgaac gccgcccgca
     3361 ggcggcgtcc gggcgaccac gggccggctc acttggcctg cgcgaccatc cattcgaccg
     3421 tggccttgac ctgttcgtcg gtcagcgccg ggttgccgcc cttggccggc atcaccccgg
     3481 catcgccggt gtagccctgg atcgcgttgg tcaccagcgt atccacgccc ttggccaccc
     3541 gcgcgccgat cccggcggca tccagcatcg gcgcgccacc ggcgccggag gtgtggcagc
     3601 cggtgcacag gttgttgtag atcacgctgc cgtcggtggt gccgccgtag gccacttccg
     3661 ccgccgccgc cgcggttgcg gcctcgagcg ccgccgcctg ctgcgcggca ccggtggcgc
     3721 cggcgtagac cgcgccggcc ggggcgatcc gctcgagcac gcgctgctgg gcggcgggat
     3781 cgggttcgcg ctgcacggtg gcattgaggt ggcgcgcgaa caggatcagg ccgacggtca
     3841 ccgccatcag gaacgcgatc accatcgaga agcgcttgag gaattccagg tcgtagttgc
     3901 gcacgatcca ccctgaggag ttgtccggcc gcggccgtca aaccgcggat tatcccagaa
     3961 ccggcgcaac cgcgcgagat cgcggcccgc ccgcctgccc cggagcgggc gtggcaacgc
     4021 atcggcggcg tggaggacac gagcggagcc ggatgtccgc ggaccctgat gcgggccaga
     4081 aagccgtgtc gcatcgcaac atgaatgcgt gtcgccgcgt tctcctggac ggccggttac
     4141 cacctccacc cgtcctgcgc ccggccgcag ccggcagtca aggttgacaa tgacagggtg
     4201 tgacggcgat aacagtcggg ttgtgcccgg gggcggggat gcccgtggca cgcatccaac
     4261 ggacgcgaca cccaccggcg caagcgcccg gggtgggggg tggcgttcct cactttgaaa
     4321 tgggatcagg gggtcatatg agcggcatga agcattcgga cgggtctgcc agcacgcggc
     4381 gtgcacgcag gatcaagctc gcgcccatga cccgcgcggt gcgcatggcg ctggctgcat
     4441 cggtcgcggc gctggcgctg ggcgccgcca ccccggcctt cgcagccggc ccgaaggtgc
     4501 cgctggaact gcagctttcg cagggtgcga tccccagcga gcgcctgcca gtcatcgacc
     4561 tgaccacggt gccgatgttc agcaccgcgg aagtcccggt cgagctgcat ccgaccgcga
     4621 tcagccagta ctccccgggc gacgtcagca tctacaacga cgccccgatc agcgagagcg
     4681 acgtcttcta cgacatggcc gcgatcagcg gctacagcga ctacggcgcg gtggagatca
     4741 ccaacaccgc caatggcgag ctgtacgccg gctccttgtt cggcgacgcg atcgggatct
     4801 acgggtactc gctgacgggc gacgtgtcga tcgacaacgc gggcgacatc accgcgtatt
     4861 cgtacgcggg cctggcggac ggcatcttcg cctcgggcgc gaacgtcgag gtggccaatt
     4921 cgggggcgat cgagagctac ggctacacct ggtcggcggg catcgaggcg cagggcgccg
     4981 attcggtggt ggtggacaac agcggcgaca tcaccgcgta cacgggtccg ttcgtgcagg
     5041 tggtcgacgg ctacggctac accatcggct acagcgccgg cggcaccgcc tacggcatct
     5101 acgcgaccgg cggcgaagcc ggcgccagcg tcaccaacag cggcaacatc gaggtgcagg
     5161 gcggctacgt caccggcatc gaagtgcagt cgagcggcga cctggtggtg gacaacagcg
     5221 gcggcatcgt cgccggctcg ggcctgaact cctactacaa cggcagcaac tactacacct
     5281 actacttcgg cacccaggtc gcgaccggca tcaacgcgac cagcaatggc gaaggcgcgg
     5341 cggtctccgt cagcaacagc ggcgacatca gcgcggacgg catcttcggc gcgaccggca
     5401 tcgcggccac cgccagcggc tacggcggtt cggccagcgt gaccaacagc ggcgccatct
     5461 acgcctcgca gaaccagaag tacggctacg gcgcgtacgg catcgtggtg tcgtcggacg
     5521 gcgacggctc gatcgacaac agcggcagca tcgaggtgta ctcgggcggc gtggccaacg
     5581 gcgccaccgt gctgtcgttc gccggcgacg ccagcgtcac caactcgggc gacatctcgg
     5641 tctacagcgc ggcggcgttg tactacgccg cgaccggcat caccgcgttc gcggccaatg
     5701 gcagcgcgca cgtcgacaac agcggctcgg tcgacgccag cgcgaagtac tgggcgaccg
     5761 gcgtgaacgc gcaggcttac ggcgacgtca ccgtcgacaa cagcggctcg ttgtacgcgg
     5821 acgggcagaa gtacgcgttc ggcgtgtacg cggcgtcgaa cgcgggcgac gtcgtggtgc
     5881 acaacgaaga cggcggcgag atcggcttct actcgtacct gggccgtggc tggggcgtgt
     5941 tcgcctacgc cggccagggc gacgtgcacg tggacaacgg cgggacgatc gcgggttatg
     6001 cgtatggtca gtcggcgggc gtgttcgggc tcgccggcgc gggcaacgta tcggtcgaca
     6061 acagcggcac gatcgacgtg gtcagcggcg gcggcaccgc ggtgggcgtg ttcgcgcgcg
     6121 ccgactacgg caccgccagc gtcagcaaca gcggtgacat cacggcctcg gtaggtagtt
     6181 attactacag tggtttcgcg gcatacggcg tgctcgcgcg cggcgcatat gccgaggttt
     6241 ccaacagcgg caacatcgtc gccgagggtt actactacgc caccggcatc gcggccacgt
     6301 cctactacgg gacccttgtg cacaacacgg gcggcagcat cagtgcctac gcgctcggtg
     6361 gcgccaccgg catcgacgcg ctgtcgctgt tcggcgacgt ggtcgtcgac aacgccagcg
     6421 acatcgaggc ggtcgggctg gtgttcggtg cgcagggcct ccgcgcctac agcgtttacg
     6481 gcgatgtcga aatccacaac acgggcgata tctacgccgg ctccctgtac ggcgacgcga
     6541 tcgggatcta cgggtactcg ctgacgggcg acgtgtcgat cgacaacgcg ggcgacatca
     6601 ccgcgtattc gtacgcgggc ctggcggacg gcatcttcgc ctcgggcgcg aacgtcgagg
     6661 tggccaattc gggggcgatc gagagctacg gctacacctg gtcggcgggc atcgaggcgc
     6721 agggcgccga ttcggtggtg gtggacaaca gcggcgacat caccgcgtac acgggtccgt
     6781 tcgtgcaggt ggtcgacggc tacggctaca ccatcggcta cagcgccggc ggcaccgcct
     6841 acggcatcta cgcgaccggc ggcgaagccg gcgccagcgt caccaacagc ggcaacatcg
     6901 aggtgcaggg cggctacgtc accggcatcg aagtgcagtc gagcggcgac ctggtggtgg
     6961 acaacagcgg cggcatcgtc gccggctcgg gcctgaactc ctactacaac ggcagcaact
     7021 actacaccta ctacttcggc acccaggtcg cgaccggcat caacgcgacc agcaatggcg
     7081 aaggcgcggc ggtctccgtc agcaacagcg gcgacatcag cgcggacggc atcttcggcg
     7141 cgaccggcat cgcggccacc gccagcggct acggcggttc ggccagcgtg accaacagcg
     7201 gcgccatcta cgcctcgcag aaccagaagt acggctacgg cgcgtacggc atcgtggtgt
     7261 cgtcggacgg cgacggctcg atcgacaaca gcggcagcat cgaggtgtac tcgggcggcg
     7321 tggccaacgg cgccaccgtg ctgtcgttcg ccggcgacgc cagcgtcacc aactcgggcg
     7381 acatctcggt ctacagcgcg gcggcgttgt actacgccgc gaccggcatc accgcgttcg
     7441 cggccaacgg cagcgcgtat gtcggcaaca gcggctcggt gtcggtgacg tcggcggcga
     7501 tcctgggcgc ggcgacgcgt gcggtcgatg cgcagggcca gcagggcgtg gtggtggtca
     7561 acagcggctc gctgtacgcc aacggcaagt acgcctatgg cgtgtatgcg ctgtccaccg
     7621 caggcgacgt ggcggtgcac aacgaagacg gcggcgagat cgggttctac tcgtactcgg
     7681 gcaccggctt cggcgtgctg gggatcagct acctgggcga cgtcgacgtc gacaatgccg
     7741 gcagcatcga aggctatgcc tacgggcagt cggtgggcgt gttcggcttc tccggtgatg
     7801 gcgacgtcag cgtccacaac agcggctcga tcagcgcggt caccggtggc aacgcggcgg
     7861 tgggcgtgtt cgcccgcgcc gactacggca ccgcgagcgt cagcaacagc ggcgacatct
     7921 acgcgggcgt gaacggcgtg aacggctaca tcggcgacgt cgcgtacggc gtcctggccc
     7981 gcggcgaata cgcggacgtc ggcaacagcg gcagcatcga ggcggtcggc ttctactacg
     8041 ccaccggcat cgccgcccgt tcgtattacg gcaccaccat cgccacctcg gcgagcagcc
     8101 agatcgaagc caccgcactg ctagtggcca ccggcatcga aggccgcgtg gaattcggca
     8161 acgtggtcgt ggccaatgcc ggcgacatca tggtggaagg cgtgtatggc ggcggcgtcg
     8221 gcatccaggc ctacgcgtac ttcggcagcg tgtccgtcgc caacgcgggc gacatcctcg
     8281 ccggctcgac ttacggcacc gcgatcggcg tgaccggagc cgtggtattc ggcaacaccg
     8341 tggtctccaa cagcggcacg atcgaggcgg ccgggtacta cggcgcctac ggcatcgcgg
     8401 cgcagtcgta cttcggcgac gtcaccgtca gcaacagcag cagcggccac gtccatgcgg
     8461 cggccccggg ctatgcctac ggcgtgttcg ccagtgccta cggcgacgtg accatcaaca
     8521 atgcgggcga cgtctcggcc agcgccgatg acggtggcag cgcggcggcg gtgctgatgg
     8581 aatcgctgta cgggcacagc acgctcaaca atgccgcgac cggctcgatc agcgtcgaag
     8641 gcaccgacgg ttatgccttc gccgtggtcg gcagcgacgc ggtcgacacc atcaacaaca
     8701 gcggccacat ctacggcgcg atctccctgc tgggcggcaa tgacgccttc aacaaccggg
     8761 cgggcggcct gtgggacgtc gacgggaccg tctccacgga tttcggcgac ggcaacgaca
     8821 cgcttggcaa cgcagggtcg gtgcggatcg acgatggtgg ggcaatcagc ttcggcgctg
     8881 gagatgacag catcaacaac ctcgccggtg gcgtgatccg cctcgccggc ggctcgatct
     8941 acatgggtgc gggcaatgac ggcatcctca acctcgggac catcgcggtc gcgggcgatg
     9001 acaacctgat cgaccttggc ggtggtgccc aggatgtgct gaccaacgtc aacaccatca
     9061 atttcctgga cggccatacc gatgacgcgc tggcgatcca cggcaacctg ggcggcaccg
     9121 gctcgctcgg gctggacgtc gacctcgccg acgagagcag cgaccagctg tacgtggaag
     9181 gcagcatggt gagcaacgcg gcgcagcgcg tgaacatcgc gttcgccggg atgccgctgg
     9241 tcgccagcac ctcgatcgac ttcgcccacg tcagcggcac ctccgccgcc ggctcgttcg
     9301 tgccgggcca gatcctgggc tacaactacg cgcgcaactt cctcgacctc gccttcacca
     9361 tcggcagccg catcaatacc gccaacaccg ccgacgacgt gttctcgatc gagctcgacg
     9421 tggtgggcct gaacgacacc ggcacgctgg cggcgaacgt ggcaagtggc gccgcgggca
     9481 tgctcaatgc gcaggtcggc accttcaagc agcgcatggg cgtgaacccc tacggcgatg
     9541 acggcaaggt gatgagcgcg ttcttccgca cctacaccag cgaaggcgac gtcgatccgg
     9601 cgcacatggc ggccaacttc ggccagggcg gcaatttcgc gttcgaccag accacgtggg
     9661 gccgggaagt cggcgtcaac gccaacctgc acggcaactt ccacgccggc gtgaccctgg
     9721 gcagcgccga cggccgccag cgcctgaccg gcaatggcgt cggcgagaac cgcatggatg
     9781 gcatgacctg gggctggtac gcgacctggt tcgcgccgca gggcttctac gtcgacctgt
     9841 ccggccgctg gatggcggtg gacgtgcgct ccacctcggc cgcgggccag ctggaatccc
     9901 gcgcgcacac cggtgcgtgg aacctcgaag ccgggtacca gtggaccctc gctggcctgt
     9961 cggtggtgcc gcagttgcag tacacccgca ccgaagtcga ggacgtgcgc acgatctatg
    10021 gcgaccgtgc caatttcgaa ggccatggcg gcacctcgtc gcgcggccgc cttggcgtcg
    10081 agatcagcaa gaccttcgaa tccggcgaca tgcgctggac gccgtacggt tcgctcaacg
    10141 cgatccgcga gttcgatggc gaaatgtcct acaccgtcgc cgacaacttc ttcggcagca
    10201 ccgataccga aggcaccagc gtgatggcgg aactgggcct gggcgtgcag aagggtggtt
    10261 ggggcttcac catcggcgcc aactgggccg atggcggcgc cttccagagc accgtgagtg
    10321 gccaggcgct ggtgcgcttc gcctggtgat gcaacacacg gacccggcgc cgtcgccggg
    10381 tccgtcgtcc aatgagtctg gagtacccgg ttaagtgtcg acagagaaaa ccccggacgc
    10441 aggtccgacc cagcctggca atgcgacgcc ggagctggag accggacttg atacggtcgg
    10501 tatcgaacct cccgccgccg cacccgccgc ggcgaatgcc ggcaagccgc cgcgcgacct
    10561 caccaagctc aaggtcgaga agggttgctc tcgcgggctg gctggctggc tcgggcagca
    10621 ccggctgtcg ctggcgatca cctcctacca gtccggccgc atctaccttg tcggcagcga
    10681 caagcagggc cgcgtctcgt tcttcgaacg catcttcgag cgcgcgatgg gcgtggtcgg
    10741 caatgcccag cgcatctacc tgggcggcct gtaccagctg tggcggttcg agaacgtgtt
    10801 gcgcccgaac gaggccatcc acggccagtt cgacaagtgc tacgtgccgc gcaacgcgca
    10861 gaccatcggc gacctggaca tccatgaact tggcatccag gccaacggca aggtcatctt
    10921 cgtcaacacc aagtactcgt gcctggccga actgagccag acccacagct tcaaggcggt
    10981 gtggaagccg aagttcatca gcaagctcgc ccccgaggac cgctgccacc tcaacgggct
    11041 ggcgatggtc gagggcaggg ccaagtacgt caccgcggtg tgccgcagcg acagcgtgga
    11101 cggctggcgc gaccgccgcc aggacggcgg ggtggtgatc gacgtcgaga ccgacgaaat
    11161 cgtctgcgaa gggctgtcga tgccgcattc gccgcgctgg cacaacggca ggctgtggct
    11221 gctgaatgcc ggcaccggcc acctcggctg ggtcgatttc gagaagaagg ccttcgtccc
    11281 gcacactttc gtgccgggct tcgcgcgtgg cctgtcgatc atcggcaacg tcgccgcggt
    11341 cggcctgtcg aagccgcgca accagcgctt cgagggcctg cagctggacg aggaactgaa
    11401 gaagcgcgat gccgagccgt ggtgcggcgt gcagatcgtc tcgctggcca atggcgacgt
    11461 gatgaactgg atccgcttcg agggcgacat caccgagatc ttcgacatca gcttcctgcc
    11521 gaacgtcaag aacccgatga tgatcggcct gcgcaccccc gagatccgcg acctgatcac
    11581 cttcgaatcc gaacttgcgg tggaggcgcc tgccgcatga accgcgcaac cttcctgctt
    11641 gccgcgatga tcgcgctgtg cgggccgcag gcatgggcgc aggcggcgaa gcccgcgccg
    11701 cagcccgcgg ccgcggatcc ggcggcggag gcgttcaagg cctgggacaa ggacaagaac
    11761 ggcaacctgt cgctggtgga gttccgcgcc ggctggcagc aggtgcaacg ggtcgccgaa
    11821 acgcaggcgc gcctgcgcca gcagttcgcg gccgtggaca ccaaccgcaa cggcgcgatc
    11881 gatcccggcg aatacggcag cctggtgctg gtaaagcagg ccggcaagaa cgcgccgcag
    11941 atgtccacct tcgacggcaa ccgcgacggc aagctggggt tcggcgagta cgtcaagctg
    12001 gtgcaggcgc tggccccgaa agaacctgcg aaggccgcgg cgaaatgaag gtcgacatcg
    12061 tcccctgctg gaagcaggtc aggccggagc tggcgcagga gctgatggcc ttctggcgcg
    12121 acaacaaggc gatcggcgac gacgccgccg cggccgcacg cgcgatgcag gcggtttgcg
    12181 tggcccgcga cgaatccggt gcgctgtgtg gcgtcggaac cgcggtggtc aaggtgctgc
    12241 cgcggctgcg ccagccgatg tactactacc gccagtactt cgccaagggc ctgcgcggcc
    12301 agcaccagga actgccgttc tacctgcgtg cgaagcaggt cctgcaggac tacaacgcct
    12361 ccctggaccg acccgaaagc ctgggcatcc tgctcgaggt cgagaacagc aagatcgccg
    12421 ctgcctacaa gcacgcctac gagccggcgt tcgacgccac cttcatcggc tattcgccgc
    12481 gcggcctgca gctgcgcgtg tcctacttcg acggcgccga actgctggca ccggcgccgg
    12541 cgccggcgcc ggtgcgcggc acggcaatgg ccgcgcgcgc taccatcccc gatccaaccc
    12601 gcatccaacc cgcttcccgg cgcggcaacg cccggtcctg accaccacgg agaacaccgc
    12661 catgaacgtc cagatgtccc tgttgagcct cgccgtcgtt tccgggcttg ccatgtcggc
    12721 tcccgcggaa gcggccggca accagaagtc cgccgccatt gcccgcgcca acgcgctgat
    12781 cgcaggtccc gccgcggcaa gcgtgcgccg cgccggcgcc gacggcttcg tcgcccgcga
    12841 cgtggtggtc gatgccgacg gcaccgaaca cgtgcgcttc gagcgcacct accagggcct
    12901 gccggtgatc ggcggcgact tcgtgctgca gtcgcgcaat ggcaaggtcg gcaaggtcag
    12961 ccagaccctg aagaccagcg cccgcccggg ccttgccggc cgcatcggcg ccgaccaggc
    13021 gatcgtcgaa gccggtgccc gcttcggcag cacgttcatc ggcgcgccca cctcgcgcaa
    13081 ggtcgtgtac gccctgggtg cctcgccggt cctggcccat gaggtcgtgt tcaacggcgt
    13141 gaaggccgac cagaccccga cggtcatgca ctacatcgtc gacgcggcga acggcaaggt
    13201 gctgaaccag tgggacgaga tccacaccgc gcgcccgggt ccgggtggtt cgtcctgcgc
    13261 cggcaccgcc gctgccgcca ccggcgcgtc gctgatggtc ggcagcgtgg ccctgaccac
    13321 caccaagtgc agcgccacca gctacgagat gcgcgatccg acccgcggcg gtggctacgt
    13381 caccaacatg gcgatgaaga cctccggcaa cggcaccgtg ttcaccgatt ccgacaacgc
    13441 ctggggcaat ggcctgacca gcaattcgca gacggtcgcg gtggatgcgc agttcggcat
    13501 cgccaagacc tgggactact tcctgaccgt gcacggccgc aacggcatcg ccggcgacgg
    13561 ccgcggttcc ttcagccgcg tccactacgg ccgcaactac gccaacgcgt tctggagcga
    13621 cagctgcttc tgcatgacct tcggcgacgg cgacaacggt gcgtcgatct atccgctggt
    13681 gtcgatcgac gtcgccggcc acgagatgag ccatggcgtg accagccgca ccgccaacct
    13741 gatctacaac ggcgaatccg gcagcctcaa cgaagccacc tccgacatct tcggcacgat
    13801 ggtcgagtac tacgcgaata ccgccgcgga cccggccgac tacgtgatgg gcgaggagct
    13861 gtacccgaac aacgccaaca tgtccaatgc gatccgctgg atgttcaagc ccaccctcga
    13921 caactattcg cagcactgct acgacccggc caccacgccg acgttcgaag tgcacggtgg
    13981 ttcgggcatc gccaacagct tcttctacct gctggcggaa ggtgcggtgg tgccggccgg
    14041 gttcggcgcc ggttcctggg ccaacctgtc ggcatcctcg ctggtgtgca acggcaacac
    14101 cgcgctcacc ggcatcggcc gcgatgcggc aggcaagatc tggtaccgcg cgctgaccgt
    14161 gtacatgacc tcgggcacca actacgccgg tgcccgcgcc gccaccatca gcgctgccac
    14221 cgacctgtac ggcggcacct cggcgcagac caacgcggtc aaggcggcgt ggagcgcggt
    14281 caacgtcaac tgaccgggga ccacggcaaa ggatgacgaa cggcccgcgc aagcgggccg
    14341 cttcgtttgg agcgggggaa tcacgacgaa gcgccgtcgg agatggcgcg ccgggagggg
    14401 tgagtcgatg cggctgcgaa ccgcaggttc gc
//
LOCUS       DAORUK010000049        14396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_4053_length_14396_cov_21.003556, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000049 DAORUK010000000
VERSION     DAORUK010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 14396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14396
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4053_length_14396_cov_21.003556"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /gene="ppnN"
                     /locus_tag="PK818_04945"
     CDS             <1..804
                     /gene="ppnN"
                     /locus_tag="PK818_04945"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017154671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HQY82117.1"
                     /translation="PGAMKGPMKGATIAHAKQRHRRTRYIGITEPGIIAAESPNPIVN
                     HLVIMPDIEKRLEAFVRMAHGIIVFPGGVGTAEEILYLLGILLREENAGRRFPVILTG
                     PTGAAAYFEQIDRFIRLTLGEEAATRYEIIVDDPEAVARRMQAGIRKVREQRIAQKDS
                     FFFNWAMHVPLAFQQPFAPTHEAMAALDLHHGRKPHELAADLRRAFSGIVAGNVKEDG
                     MRRIEQFGPFEIHGDPDIMQALDGLLRAFVEQRRMKIAGDYRPCYRVVT"
     gene            complement(811..1836)
                     /locus_tag="PK818_04950"
     CDS             complement(811..1836)
                     /locus_tag="PK818_04950"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQY82118.1"
                     /translation="MSTTAATPLGVLFRPQAIIATILVGEAFAVVLALSPAHSPDRLV
                     HFGLASLAIQWVAMGTLAVLHVLRKHLARLPAARLAWVALGSFISISVLVAWGAVLLA
                     ELAVPLPEARTSFLLRWGCIALVVGLLALLTYRNYWNARQLAVRTKQLELESLRARIR
                     PHFLFNTLNTSAALVHARPDEAERVLLDLADLFRAALRGPERVPLAKEVELTRRYLEI
                     ESFRFGPRLRVAWSLPDPLPDANVPALSLQPLAENAIRHGIEHMPGGGNIDIRLRHAS
                     GWITIEVENDMPADGNRATDGHRIGLNSARERVRALSAGRGDVETRLVNGRHLARLTI
                     PEDVPAA"
     gene            1975..2511
                     /locus_tag="PK818_04955"
     CDS             1975..2511
                     /locus_tag="PK818_04955"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HQY82119.1"
                     /translation="MRNRQRYVRDWARVRGFTVIELMVTVAVIGVLAVVAVPAMSGLV
                     NAYRLNGAAGELQTAIQLAKSESVRRNVPVTVCASADGTTCSGATAWSRWIVRGRDPG
                     TVNTIDIMRDETPASGIVVSGPAAGIVFRSSGRIDAQAAVVACKAVTNPAQNQRAVTV
                     LISGVSNITKSNGGGACP"
     gene            2544..2993
                     /gene="pilV"
                     /locus_tag="PK818_04960"
     CDS             2544..2993
                     /gene="pilV"
                     /locus_tag="PK818_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017112417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HQY82120.1"
                     /translation="MPSRRLPSASRSRVSGLSLIEVLVSVLILGIGLLGIAAMQSMAL
                     RGGQSSLESSQVVISTNSIIEAMRANRANAANYNTGGKRCAVTGGATLAGNDVDNWIT
                     SLQTSMPGNTTCGTIAGCPNACVITVEWDDSRALGGAARTMVTEARI"
     gene            2990..3877
                     /locus_tag="PK818_04965"
     CDS             2990..3877
                     /locus_tag="PK818_04965"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQY82121.1"
                     /translation="MKREPANRAVARRSLGFGLIELMIAMVLGLIVLGAAIAVFQSNQ
                     RSYSANEGQNRVQENARAAYEMVARDLRATGGSACSSESLVMGADAESVSFRAPLAGS
                     ATEVTTTSADDLSYRIVAGSASANSVTLVEATPAASDVFRANDIVMVCNAAMTGFATV
                     SSVSGQTITFSGALPFDPKDTDNADPGSISIARLRSNRWFTQANGRDTGTSLYMSRSG
                     GAAEEVADGVQSLGITYHQASGGNPNVYVAAPTSMQRVDAVRMSMPIRAVMPTQNPGE
                     SRNVDRTIATSVAIRSRAQ"
     gene            3874..4431
                     /locus_tag="PK818_04970"
     CDS             3874..4431
                     /locus_tag="PK818_04970"
                     /inference="COORDINATES: protein motif:HMM:NF025061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HQY82122.1"
                     /translation="MNARLVSRRRAPARQQGVSLLVVLILMVVMSVLGIAVLRSSAMQ
                     ERMSANMYDRSLATQAAEAALVEARAWLGSGSGVDWSTQQPADVAGACANFSVCPTYT
                     SQAAAVWRAGPTLMAANPGLRTTTQFWIEYLGQNQSHMSSGGVVPSSATTSLGPMYRI
                     TARSQGQGRASVTLQTDVIYRFPTL"
     gene            4452..8012
                     /locus_tag="PK818_04975"
     CDS             4452..8012
                     /locus_tag="PK818_04975"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HQY82123.1"
                     /translation="MNKRRSIPLNAKSAWALPAAFMATLLGMPVHAGITIPDEPLTTA
                     NRVAPNILFILDDSGSMDDLNMPDSVPTTSYVDVSMTAYPRNTLSYNPSVAYQTWTLA
                     DGNRRTGGTSYTSAYSDLNLASGSVNLCSSTQTFHSPKNPTSAVPGYFGDGRNYYRFQ
                     IPAGCTQVVRSEYGAVVATSWTPVAITPATGSVNNSSATHTLASIVAGRELQVTITST
                     NSRQADWSVRDPGNNSVCSGSVDDGEVDTCNIAPTVAGVYTVRMSRGTTGSSTYSLSA
                     RSRSNNRCPTGGTGSNDWINCTPATPTGRSVADEKTNYATWYSYHRTRIKAAKAGASD
                     AFKPLGNRVRVGYRSIWDRSNFDIPVGDGNDGRFVDNIDDPGTPVNEATTSRSTWYNR
                     LHAAVGSGRTPLKPALNSAGQYFQNTASTGPYGPQATASQYSCRQNFTILTTDGYWND
                     GTVSVGNADNTSGPNHTDTAGNSFGYTAAAPHRDGFSNTLADVAMYYWKRDLRTESNM
                     VNNVPTTSSDPAFWQHMVTFGISIGLSGNKGWSTISDVPANATWEDPNDAEDGDRIDD
                     LLHAAVNGHGTFVTASSPSEFTAGLTQALAAISQRTSSFSNVATNAASVRTGGKVFSA
                     SYVSGLWTGTVKAWTLDAYNNPTTLAWNASVPGIATRKVYTFDGFGGQLFPTSSQISS
                     LTRTGGPVDYPVTGSVNASYLKGDASNEERNGGLLRNRTSVMGDIVGSSPAYVDDTST
                     LYVGANDGMLHALDAGTGQELFAYVPSIINMAHLRDLSRGDYTHKFFVDGPVVVTNRK
                     LTPGKNLLVGALGKGGRGLYGLDVSSPGTFDGSGVKWELAQTSGNNMGLVTGRPILAK
                     VKSGAVAAILGNGVNSPNDKAVLIVVNAETGAVIREIDTGAGSAGAPNGLAAPTAVLG
                     PDGKAVAYAYAGDRLGNVWRFDLTSSSPSGWSATKLFTAKSNDGAGAVQPITGGVTVA
                     TDPATFKRWVFLGTGSFITTTEADDKTPLAQSMYGFIDDGTASVVAYSDLVKRTIGNT
                     GATQDGYPVRTFEAKSSLPDNKKGWFVNLPGNGERIVQDAQVVANILVTASMIPEGDA
                     CEASGTGYINALDAFTGTSAGSSFFDLDGDGKTDDTMVGGVPVGSVNFGVGMPTLPIF
                     LDGKLVVGGTGGSVSAEKPGAGGITRKSWGRVSWRELRAD"
     gene            8012..8431
                     /locus_tag="PK818_04980"
     CDS             8012..8431
                     /locus_tag="PK818_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HQY82124.1"
                     /translation="MESVQMPGMPRATARVGGFSLIELMVVVAIIGILASIALPSYND
                     HQRKTRRAAGAACATAVAQQFERFYTSNLTYVGAVANTAICDPEVLTFYNVTAGGVAA
                     KTYTISAAPTGKQAGDGCGSLSINQAGTKSPATAGCW"
     gene            complement(8699..8775)
                     /locus_tag="PK818_04985"
     tRNA            complement(8699..8775)
                     /locus_tag="PK818_04985"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8739..8741),aa:Asn,seq:gtt)
     gene            complement(8833..9402)
                     /locus_tag="PK818_04990"
     CDS             complement(8833..9402)
                     /locus_tag="PK818_04990"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HQY82125.1"
                     /translation="MGEHWPQFIGSLAMLRRNAGFTLIEFATCVAVMAICAGIALPSM
                     GSFVERQRASAAAASLMAHMALARTSAISRNRRAVLCPSADGNFCEADNDWSRGWMLF
                     LDDDGNRKPDKNDEIIRVELEPTSRHLQVTSTNGRQQLRYLPDGRSAGTNLTISICNS
                     RQELLGQVIVNNMGRPRSERPSARMPCPG"
     gene            9551..11599
                     /gene="uvrB"
                     /locus_tag="PK818_04995"
     CDS             9551..11599
                     /gene="uvrB"
                     /locus_tag="PK818_04995"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HQY82126.1"
                     /translation="MSQSEPSDLDLSTASGRFRLVAPYEPAGDQPQAIGKLVAGFENG
                     IAKQVLLGVTGSGKTYTIANMIQAVQKPTLVMAPNKTLAAQLYGEFRAFFPHNSVEYF
                     VSYYDYYQPEAYIPSSDTFIEKDSSINDHIEQMRLAATKALLSRRDTIVVATVSAIYG
                     LGAPEDYLAMRLILSRGERIDQRELLRHLTTLQYTRNEMVLDRGTFRVRGETIDVFPA
                     ESDLEALRIELFDGEVEQLSMFDPLTGEVLRKLPRYTVYPKTHYATPRERTLSAVETI
                     KAELRERQEHLYSANKLVEVQRLSQRTQFDLEMMAEVGYCNGIENYSRHLTGRAPGEA
                     PPTLFDYLPADALLVVDESHVTIPQIGAMYKGDRSRKETLVEFGFRLPSALDNRPLRF
                     EEWEQRAPRSIFVSATPGPYELREAADEIVELVVRPTGLVDPQVEIRPVATQVDDVLS
                     EIHERVAMGDRVLITTLTKRMSENLTEYLSEHGVRVRYLHSDIETVERVEIIRDLRLG
                     MFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSAGSLIQTIGRAARNLRGKAILY
                     ADRVTNSMQKAIDETDRRRAKQVEYNAEHGITPQSIVRAVADVMEGARPEPGELKGRG
                     KLRRVAEPELEYQRLSPAQQAAKLKALEQQMHQHARDLEFEDAARVRDQIRRLREASL
                     "
     gene            11712..11786
                     /locus_tag="PK818_05000"
     tRNA            11712..11786
                     /locus_tag="PK818_05000"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11744..11746,aa:Val,seq:gac)
     gene            complement(11757..12008)
                     /locus_tag="PK818_05005"
     CDS             complement(11757..12008)
                     /locus_tag="PK818_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82127.1"
                     /translation="MIEIMGEPFGDAGPVAGVGNAENEKGAEAPFDYSRMNRMRRQVQ
                     RWMRVVLMSHARPALPQATLFPIRCHARKAWWAVQGSNL"
     gene            11995..13896
                     /gene="thrS"
                     /locus_tag="PK818_05010"
     CDS             11995..13896
                     /gene="thrS"
                     /locus_tag="PK818_05010"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019303507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HQY82128.1"
                     /translation="MISITLPDGAVRQFEAPPSVHDVAASIGAGLAKATLAGKVDGRL
                     VDASHVIDRDASLAIITEKSPEALEILRHSTAHLLAQAVQRLFPGAQVTIGPVIDNGF
                     YYDFAYERPFTPEDLPAIEAEMQKIVKEALPLSRSVKSRDEAVAYFKGIGEAYKAEII
                     ESIPANEELSLYSQGEFTDLCRGPHVPGTDKLRAFKLMKVAGAYWRGDHNNQMLSRIY
                     GTAWLNDKDLKAYLTQIEEAEKRDHRKIGKALDLFHLQEEGPGLVFWHPKGWSIWQTV
                     EQYMRKVYRDTGYGEVRCPQILDVTLWQKSGHWDNYKDNMFFTESEKRTYALKPMNCP
                     GHVQVFNQGLHSYRDLPIRYGEFGACHRNEPSGALHGILRVRGFTQDDGHVFCTEAQI
                     EPEVRAFHEQALKVYSDFGFSDIQIKIALRPESRLGDDATWDKAEDALRNALRASGVE
                     WQELPGEGAFYGPKIEYHLKDAIGRTWQLGTMQVDFMMPGRLGAEYVDEHSQRRHPVM
                     LHRAIVGSMERFIGILIEHHAGHFPAWLAPVQAVLMNITDAQADWVEEARKTLANQGF
                     RVVSDLRNEKIGYKIREHTLQRVPYLLVVGDREKENGQLSVRTRGGEDLGTMSVADFA
                     ARLRQEQAA"
     gene            13981..>14396
                     /gene="infC"
                     /locus_tag="PK818_05015"
     CDS             13981..>14396
                     /gene="infC"
                     /locus_tag="PK818_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HQY82129.1"
                     /translation="MSTAPDSKQNRRNLEIRVPRVRVIGSDGEMIGVLSRDEALRMAE
                     EEALDLVEIQPNADPPVCKIMDFGKFKFELQKKANEAKKKTKQVEIKEVKFRPVTDEG
                     DYQIKLRKMREFLIEGDKIKVNIRFRGREMSHQELGR"
BASE COUNT         2672 a         4643 c         4746 g         2335 t
ORIGIN      
        1 ccgggcgcga tgaaggggcc gatgaagggc gccaccatcg cccacgccaa gcagcgccac
       61 cgccgcaccc gctacatcgg catcaccgag ccgggcatca tcgccgccga atcgccgaac
      121 ccgatcgtca accacctggt gatcatgccg gacatcgaga agcgcctgga ggccttcgtc
      181 cgcatggccc acgggatcat cgtgttcccc ggtggcgtgg gcacggcgga ggagatcctg
      241 tacctgctcg gcatcctgct gcgcgaggaa aacgccggcc gccgcttccc ggtgatcctg
      301 accggcccca ccggcgccgc ggcgtacttc gagcagatcg accgcttcat ccgcctgacc
      361 ctgggcgaag aggcggcgac ccgctacgag atcatcgtcg acgaccccga agccgtggcc
      421 cggcgcatgc aggccggcat ccgcaaggtc cgggaacagc gcatcgcgca gaaggattcg
      481 ttcttcttca actgggcgat gcacgtcccc ctggccttcc agcagccgtt cgcacccacc
      541 cacgaggcga tggccgcgct ggacctgcac cacggccgca agccgcacga actcgccgcg
      601 gacctgcgcc gcgcgttctc cggcatcgtc gccggcaacg tcaaggaaga cggcatgcgc
      661 cggatcgaac agttcggccc gttcgagatc cacggcgacc ccgacatcat gcaggcgctg
      721 gatggcctgc tgcgcgcctt cgtggagcag cggcggatga agatcgccgg ggattaccgg
      781 ccgtgttacc gggtggtgac ctgaccctgg tcaggctgcc gggacgtcct cggggatcgt
      841 caatcgcgcc aggtgccggc cattcaccag ccgtgtctcc acatccccgc gcccggcgct
      901 gagcgcccgc acgcgttcgc gcgcggagtt caggccgatc cgatggccat ccgtcgctcg
      961 attgccgtcg gccggcatgt cgttctcgac ttcgatggtg atccagcccg acgcgtgccg
     1021 caggcggatg tcgatgttgc ctcccccagg catgtgctcg atgccgtggc ggatcgcatt
     1081 ctccgcgagc ggttgcaggg acagtgcggg gacgttggcg tccggtagcg ggtccgggag
     1141 cgaccacgcc acgcgcaagc gggggccgaa gcggaacgat tcgatctcca ggtagcgccg
     1201 ggtcagttcc acttccttcg ccagcggaac ccgctcggga ccgcgcaagg cggcgcggaa
     1261 caggtccgcg aggtcgagca gcacgcgttc ggcctcgtcc ggccgagcgt gcaccagggc
     1321 tgcgctggtg ttgagcgtgt tgaacaggaa gtgcggccgg atccgcgcac gcaaggattc
     1381 cagttcaagt tgcttggtgc gcacggcaag ttggcgcgcg ttccagtaat tgcggtatgt
     1441 cagcagcgcc aggaggccca ccaccaatgc gatgcatccc cagcgcagca agaaggacgt
     1501 gcgcgcctcg ggcaggggga ctgcaagctc cgcaagcagc acggcgcccc atgcgaccag
     1561 caccgatatc gaaatgaacg accccagcgc cacccacgcc aggcgtgcgg ccggcaagcg
     1621 ggccaagtgc ttgcgcagga cgtggaggac cgccagcgtt cccatcgcca cccactggat
     1681 cgccaacgat gccaagccga agtgcaccag gcgatccggg gagtgcgcag gcgacaacgc
     1741 aaggacgacg gcgaaggctt cgcccaccag tatggttgca atgatcgcct gggggcggaa
     1801 caggacgcca aggggcgtgg cggcggtcgt gctcacgtct gctgaacccc acgttgaacc
     1861 actcggctga aggggactgg gccgctcacc ctgcgtcttt ggccgcccat cgggtatgga
     1921 tggctttaac gcggactttc ccatagcctg cgcccactgg aaagtggggg cagtatgcgg
     1981 aatcggcagc gatacgtgcg ggattgggcg cgggtacgtg ggttcaccgt gatcgagctc
     2041 atggtcaccg tggcggtcat tggcgtgctg gcggtcgtcg cggttcctgc catgagcggc
     2101 ttggtcaacg cctaccgcct gaatggcgct gctggcgagt tgcagacggc cattcagttg
     2161 gcgaaatcgg aatcagtgcg gcgcaatgtc ccggtgacgg tctgcgcttc ggcggatggc
     2221 accacctgct caggtgcaac cgcctggtcg cgctggatcg ttcgcggacg tgacccgggg
     2281 acggtcaata cgatcgacat catgcgggac gagacgcccg caagcggcat cgtggtctcg
     2341 ggaccggcgg ccggcatcgt gtttcgttcg tcggggcgga tcgacgctca agctgcagtg
     2401 gtcgcttgca aggcggtgac caatccggcg caaaaccagc gcgcggtgac ggtgctgatc
     2461 agtggcgtgt caaacattac gaagtccaat ggtggtggtg catgtcccta gtacgtggcg
     2521 catccatttt gcgtgaaccc gcgatgccct cccggcgcct gcccagcgcg tcgcggtcac
     2581 gcgtctcagg gctgagcttg atcgaggtgt tggtgtcggt cctcatcctg ggcatcgggc
     2641 tgcttggtat cgcggcgatg cagtcaatgg cgcttcgcgg cgggcagagc tcgctcgaga
     2701 gcagccaggt ggtgatctcg accaattcga tcatcgaggc gatgcgtgcc aaccgggcga
     2761 acgccgcgaa ctacaacacc ggtggcaagc gctgtgcggt aacgggcgga gccaccttgg
     2821 cgggaaacga cgtggacaac tggatcacat cgctgcagac cagcatgccc ggcaacacga
     2881 cgtgcggaac gatcgccgga tgcccgaatg cctgcgtgat caccgtggaa tgggatgatt
     2941 cccgggcgct tggcggcgct gcgcgcacga tggtgacgga ggctcggata tgaagcgtga
     3001 gcctgcgaac cgcgctgtcg cccgaaggag cctgggattc ggcctcatcg agttgatgat
     3061 cgcgatggtg cttggcctga tcgtgcttgg cgccgcgatt gcggtgttcc agtccaacca
     3121 gcgaagttac agtgcgaatg aaggccagaa ccgcgtgcag gaaaacgcgc gcgcggcgta
     3181 cgagatggtg gcgcgagatc tccgcgcaac cggggggtcc gcgtgctcca gcgagtcgct
     3241 ggtcatggga gctgacgcag agagtgtctc attccgggca cccttggcag gttcggcgac
     3301 cgaggtgacc acgacgtcgg ccgacgacct gtcctaccgg atcgtcgccg gcagtgcgtc
     3361 cgccaattca gtcacgctgg tcgaggcaac gcccgcggca tccgatgtgt tcagggccaa
     3421 cgacatcgtg atggtttgca atgccgcgat gaccgggttc gcgaccgtga gcagcgtgtc
     3481 gggtcagacg atcacgttca gcggcgcatt gccattcgat cccaaggaca ccgacaacgc
     3541 cgatccgggc agcatttcga tcgcgcgact gcgtagcaat cggtggttca cgcaggcgaa
     3601 cggccgggat accgggacgt cgctgtacat gtcccgctcg gggggcgcgg cggaggaagt
     3661 ggcagatggc gtgcagagcc tcggcattac ctaccaccag gcgtcgggcg gcaatccgaa
     3721 cgtgtacgtc gcggctccaa ccagcatgca acgcgtcgat gcggtgcgga tgtcgatgcc
     3781 gatcagggca gtcatgccga cccagaaccc gggagagtcc cggaacgtcg accgcacgat
     3841 cgcaacgtct gtggccatca ggagtagagc acaatgaatg ccaggctcgt gtcccggcgc
     3901 cgcgcgccag cccggcagca aggcgtctcg ctgttggtcg tgctcatcct gatggtcgtg
     3961 atgtccgtgc tgggcatcgc ggtgcttcgc agttcggcaa tgcaggagcg catgtccgcg
     4021 aacatgtacg accgcagcct tgccacgcag gccgcagagg ctgcgctggt tgaggcgcgc
     4081 gcttggcttg gcagtggttc cggtgtcgac tggagcacgc agcagccggc agacgttgcc
     4141 ggcgcgtgcg cgaacttcag cgtctgcccc acctatacgt cccaggccgc agccgtctgg
     4201 cgagctggcc cgactttgat ggcagccaac ccaggcctgc gtaccaccac ccagttctgg
     4261 atcgagtacc tcggccagaa ccagagccac atgtcttcgg gtggcgtggt gccgtcgtcg
     4321 gcgacaacca gcctgggtcc gatgtatcgg attaccgcgc gtagccaggg ccaggggcgt
     4381 gcctccgtga ccctgcaaac cgacgtcatc taccgctttc caacgctgtg acatcctcca
     4441 aggctcctgc catgaacaag cgtcgttcca tcccactcaa cgccaagtcc gcctgggccc
     4501 tgcccgccgc attcatggcc accctgctgg gcatgccggt ccatgccggc atcacgattc
     4561 ccgatgaacc gctgaccaca gcgaatcggg tcgccccgaa tatcctgttc atcctggacg
     4621 attcggggtc gatggacgac ctgaacatgc ccgacagcgt gccgacgacg agttatgtcg
     4681 acgtctcgat gacggcgtac ccgcggaata cgctgtcgta caaccccagc gtggcttacc
     4741 agacctggac cttggccgac ggcaaccgcc ggactggtgg caccagctat acgagcgcct
     4801 acagcgatct gaatttggcg agcggctccg tgaatctgtg cagcagcacg cagaccttcc
     4861 attcgccgaa gaatccgact agcgcggttc ccggctactt cggcgatggc cggaattact
     4921 atcgtttcca gattccagcc ggctgcacgc aagtcgtgcg ctcggagtat ggcgccgtgg
     4981 tcgcgacgtc gtggacgccg gtggccatca cgccggcaac cggatcggtg aacaactcga
     5041 gcgcaacgca tacgctcgct tcgatcgttg ccggccgcga actccaagtg acgatcacct
     5101 cgaccaacag ccgccaggcg gactggtcgg tgcgggatcc aggcaacaac tccgtgtgtt
     5161 ccggtagcgt ggatgatggt gaggtggaca cctgcaacat cgccccgacg gttgcgggcg
     5221 tctacacggt ccgcatgtcc cggggtacta cgggttcgtc gacctattca ttgtcggcgc
     5281 gctcccgctc caacaatcgc tgcccgaccg gtgggacggg tagcaatgac tggatcaact
     5341 gtacgccggc gacaccgacc gggcgcagcg tggcagatga gaagaccaac tacgccacct
     5401 ggtattccta ccaccgcacc cgcatcaagg ccgccaaggc cggcgcaagc gatgcattca
     5461 agccgctcgg caaccgcgtg cgcgtgggct accggtcgat ctgggatcgc agtaacttcg
     5521 acatcccggt gggagacggc aatgatggcc gtttcgtcga caatatcgat gaccccggca
     5581 ccccggtcaa cgaggccacc acctcgcgca gtacctggta caaccgcttg cacgccgcag
     5641 tcggcagcgg tcgtacgccg ctgaagcctg ccttgaattc ggccggacag tacttccaga
     5701 acacggcatc gaccggcccg tatggcccgc aggccacggc cagccagtac agttgccgtc
     5761 agaatttcac gatcctgacc acggacggct actggaacga cggaacggtg agcgtcggca
     5821 atgcggacaa caccagcggc ccgaaccata ccgatacggc aggcaactcg tttggctaca
     5881 cagcggcggc gccacaccgc gacgggttct ccaatacgct ggccgacgtc gcgatgtatt
     5941 actggaagcg cgacctgcgc accgaatcca acatggtcaa caacgtgccg acgacgtcca
     6001 gcgatccggc gttctggcag cacatggtca ccttcggcat ctcgatcggc ctgagcggta
     6061 acaaggggtg gagcaccatc agcgacgtgc ctgcgaatgc gacctgggag gacccgaacg
     6121 atgccgagga tggggatcgt atcgatgacc tgttgcatgc tgccgtaaac gggcacggta
     6181 ccttcgtgac ggcgtccagc ccttccgagt tcactgcggg cctgacgcag gcacttgccg
     6241 cgatctccca gcgcaccagt tccttctcca atgtcgccac gaacgccgcc tcggttcgta
     6301 ccggtggcaa ggtgttcagc gccagctatg tctccggctt gtggacgggc accgtcaagg
     6361 cctggacctt ggatgcctac aacaatccga ccacgctggc gtggaacgcc tcggttccgg
     6421 gcatcgcgac gcgcaaggtg tacaccttcg atggtttcgg ggggcagctg ttcccgacct
     6481 cgtcccagat cagcagcctg acgcgcacgg gtggtcccgt ggactacccc gtgacgggca
     6541 gcgtcaacgc cagttacctg aagggcgacg catccaatga agaacggaat ggcggtctgc
     6601 tcaggaaccg aaccagcgtg atgggcgata tcgtggggtc gtcgccggcc tacgtcgacg
     6661 atacatcgac cctgtacgtc ggcgccaacg acggcatgtt gcatgccctc gacgcgggca
     6721 ctggccagga attgttcgcg tatgtgccgt cgatcatcaa catggcccac ctgcgcgacc
     6781 tcagccgcgg cgactacacc cacaagttct tcgtcgatgg cccggttgtg gtgactaacc
     6841 gcaagttgac accgggcaag aacctgctgg tgggtgccct tggaaagggc ggcaggggtt
     6901 tgtatggcct ggatgtctcg tcgccgggaa ccttcgatgg tagcggcgtg aagtgggagt
     6961 tggcgcagac gtctggcaac aacatggggc ttgtcaccgg ccgcccgatt ctggccaagg
     7021 tcaagtctgg agcggttgca gcgatcctcg gtaatggcgt gaacagcccc aacgacaaag
     7081 cggtgctgat cgtggtcaac gccgagactg gtgccgtgat ccgcgaaatc gacaccggtg
     7141 ccggatcagc cggagcgccg aatggccttg ctgccccgac cgcagtgctg ggtccggatg
     7201 gaaaggcggt cgcgtacgcc tacgctgggg accgtcttgg caacgtctgg aggttcgacc
     7261 tcaccagttc cagcccgagt ggatggagcg caaccaagct gttcacggca aagtccaacg
     7321 acggcgcggg tgcggtgcag ccaatcactg gcggtgtgac cgtggcgacc gatcccgcca
     7381 ccttcaagcg ctgggtattc ctcgggaccg gcagcttcat cacgacgaca gaagcggacg
     7441 acaagacccc cttggcgcag agcatgtacg gattcatcga cgatggcacc gcgagcgtgg
     7501 tggcttacag cgacctcgtg aagcggacca ttggaaacac gggcgcaacc caggatggct
     7561 acccggtgcg caccttcgaa gcaaagtcga gcctgcctga caacaagaag ggctggttcg
     7621 tcaacttgcc cgggaatggc gaacgcatcg tgcaggacgc acaagtggtc gcgaacatcc
     7681 tggtgaccgc cagcatgatc ccggaaggcg acgcctgcga agcatccggt accggctaca
     7741 tcaatgcgct ggatgcgttt acgggcacca gcgcaggctc atccttcttc gacctcgacg
     7801 gcgatggaaa gacggatgac acaatggttg gtggcgtacc ggtgggttcc gtcaacttcg
     7861 gtgtcggcat gccgaccctg ccgatcttcc tggatggcaa gctggtcgtc ggaggaacag
     7921 gtggttccgt gagcgcggaa aagcccggtg ccggcggcat cacccggaag agttggggcc
     7981 gcgtgtcctg gcgtgaactg agggccgact gatggaaagt gtgcagatgc ctgggatgcc
     8041 gcgagccact gcgcgcgtgg gggggttctc gctcatcgag ctgatggtgg tggttgcgat
     8101 catagggatc ctcgcctcca tcgcgcttcc cagctacaac gaccaccagc gcaagacccg
     8161 tcgtgcagcc ggtgcggcct gtgcgactgc ggtggcgcag caatttgagc gcttttacac
     8221 gagcaatctg acctacgtgg gggcggttgc caacaccgcc atctgcgatc cggaggtgct
     8281 gacgttctac aacgtcaccg cgggcggtgt cgccgccaag acctacacga tctcggcggc
     8341 gcccaccggc aaacaggcgg gagacggctg cggctcattg agcatcaacc aagccggcac
     8401 caagagcccg gcgaccgcgg gctgctggta attcaatcgt gtgcgcatcg gtgcgaacat
     8461 gaggccgaaa aacccgccgg aaggcgggtt tttcatgaag cgtggagccc agggtgttgt
     8521 cgaaaatgcc ctcgccaacg cgctggcgca gaaactgcaa gtttctcgtc gccgttcccg
     8581 tgccggttcc gacgccaggc agggcaagtt cgaaggaacg catcgaacat tggctgcgaa
     8641 gtggttatgc cggaaacgat gaagggccgc cgaagcgacc ctaagtcatt gaaacatttg
     8701 gcgcccgaag ttggactcga accaacgacc ccctgattaa cagtcaagtg ctctaaccgg
     8761 ctgagctatt cgggcgggga gcgcattgta gacggcctca tcggggtcgg caagccgcgg
     8821 cgcgcgttcc cgtcacccgg ggcagggcat tcgtgcgctg ggacgctcgc tgcgcggcct
     8881 gcccatgttg ttcacgatga cctggccgag cagttcctgg cgcgagttgc agatggaaat
     8941 ggtgaggttt gtccccgcgc ttcgcccgtc cgggaggtaa cgcagctgct gcctgccatt
     9001 ggtgctggtc acttgcaggt gacggctggt cggttccagc tccacgcgaa tgatttcgtc
     9061 gttcttgtcc ggcttgcggt tgccgtcatc gtccaggaac agcatccatc cacgactcca
     9121 gtcattgtcc gcttcgcaga agttgccgtc ggcggagggg cacagcaccg cacgtcgatt
     9181 gcggctgatg gcgctcgtgc gcgccagtgc catgtgcgcc atcagcgaag ccgcggctgc
     9241 agaggcgcgt tggcgttcca cgaagcttcc catcgaaggc aaggcgatgc ccgcgcagat
     9301 cgccatcacg gcaacgcagg tggcaaactc gatcagggtg aatccggcgt tgcgacgaag
     9361 catggcaagg ctcccgatga actggggcca gtgttcgccc atgcgcggct gcggcccatc
     9421 gggaggatcc tgagggcggg gtaggggaat gccgctgcgg cggcggcatc ccatgccccc
     9481 gcgcccgcag ggttcggccc ggaccggcgg ctgcgcgtaa actcaggggt tgtccgttgc
     9541 agccccgccg atgagccaga gcgaaccgtc cgacctcgac ctgtcaaccg cttccggtcg
     9601 attccggctg gtcgcgccgt acgagccggc gggcgaccag ccgcaggcca tcgggaagct
     9661 ggtggccggc ttcgagaacg gcatcgccaa gcaggtcctg ctgggcgtca ccggctcggg
     9721 caagacctac accatcgcca acatgatcca ggcggtgcag aagccgaccc tggtgatggc
     9781 gccgaacaag acgctggccg cgcagctgta cggggagttc cgcgcattct tcccgcacaa
     9841 ctcggtggag tacttcgtca gctattacga ctactaccag cccgaggcct acatcccgtc
     9901 cagcgacacc ttcatcgaga aggacagctc gatcaacgac cacatcgagc agatgcgcct
     9961 ggccgcgacc aaggcgctgc tgtcgcggcg cgacaccatc gtggtggcca cggtgtccgc
    10021 gatctacggc cttggcgcgc ccgaggacta cctggcgatg cggctgatcc tgtcgcgcgg
    10081 ggaacgcatc gaccagcgcg aactgctgcg ccacctgacc accctgcagt acacccgcaa
    10141 cgagatggtt ctggaccgcg gcaccttccg cgtgcgcggc gagaccatcg acgtgttccc
    10201 ggccgaatcc gacctggagg cgctgcggat cgagctgttc gatggcgagg tcgagcagct
    10261 ttcgatgttc gatccgttga ccggcgaggt gttgcgcaag ctgccgcgct acacggtcta
    10321 cccgaagacc cactacgcca ccccgcgcga acgcacgctg tccgcggtgg agacgatcaa
    10381 ggccgagctg cgcgagcgcc aggagcatct gtattcggcg aacaagctgg tggaggtgca
    10441 gcggctgtcg cagcgcaccc agttcgacct ggagatgatg gccgaggtcg gctactgcaa
    10501 cggcatcgag aactattcgc ggcacctgac cgggcgcgcg ccgggcgagg cgccgccgac
    10561 cctgttcgac tacctgccgg cggacgcgct gctggtggtc gacgaatcgc acgtgaccat
    10621 tccgcagatc ggcgcgatgt acaagggcga ccgctcgcgc aaggaaaccc tggtggaatt
    10681 cgggttccgg ctgccgtcgg cgctggacaa ccgcccgctg cgtttcgagg agtgggagca
    10741 gcgcgcgccg cgcagcatct tcgtgtcggc gacgccgggg ccgtacgagc tgcgcgaggc
    10801 cgccgacgag atcgtggagc tggtggtgcg cccgaccggg ctggtcgacc cgcaggtgga
    10861 gatccgcccg gtggcgaccc aggtggacga cgtgctgtcg gaaatccacg aacgcgtcgc
    10921 gatgggcgac cgcgtgctga tcaccaccct gaccaagcgg atgtcggaga acctgaccga
    10981 gtacctgtcc gagcacggcg tgcgcgtgcg ctacctgcat tcggacatcg agacggtgga
    11041 gcgggtcgag atcatccgcg acctgcggct gggcatgttc gacgtgctgg tcggcatcaa
    11101 cctgctgcgc gaaggcctgg acatgcccga ggtgtcgctg gtggcgatcc tggatgcgga
    11161 caaggagggc ttcctgcgca gcgccggttc actgatccag accatcggcc gcgccgcccg
    11221 caacctgcgc ggcaaggcga tcctgtacgc cgaccgggtc accaattcga tgcagaaggc
    11281 gatcgacgag accgaccgcc gccgcgcgaa gcaggtcgag tacaacgccg agcacgggat
    11341 caccccgcaa tcgatcgtgc gcgcggtggc cgacgtgatg gaaggcgcgc gcccggaacc
    11401 cggcgagctg aaggggcgcg gcaagctgcg ccgggtggcc gaacccgagc tggaatacca
    11461 gcggctgtcg cccgcgcagc aggcggcgaa gctcaaggca ctggagcagc agatgcacca
    11521 gcacgcccgc gacctggagt tcgaggacgc cgcccgcgtg cgcgaccaga tccggcgcct
    11581 gcgcgaggcc agcctgtagg gcggggcaag ccccgatcca caggtgcgag caatgcccaa
    11641 ccgcctgccg tacgggcctg gcgagccccg gttgtccggc gcccgccgcc gggctagaat
    11701 gcgcgtcctt cgggcggtta gctcagcggt agagcactac cttgacatgg taggggtcac
    11761 aggttcgaac cctgtaccgc ccaccacgcc ttccgggcgt ggcaacgaat cggaaacaag
    11821 gtggcctgcg gcaacgccgg tcgagcgtgc gacatgagca ctacccgcat ccagcgctga
    11881 acctgccgac gcattcgatt catccgtgaa taatcgaaag gcgcttcggc gcctttttcg
    11941 ttttccgcat ttccgacgcc ggccacgggg ccggcatcac cgaagggttc tcccatgatc
    12001 tcgatcacgc tccccgatgg cgccgtccgt caattcgaag cgccgccgtc cgtgcatgac
    12061 gtcgccgcct ccatcggcgc cggcctcgcc aaggccaccc tggccggcaa ggtggacggc
    12121 cggctggtcg acgccagcca cgtcatcgac cgcgatgcca gcctggccat catcaccgag
    12181 aagtcgccgg aggcgctcga gatcctgcgc cactccaccg cgcacctgct ggcgcaggcc
    12241 gtgcagcgcc tgttccccgg cgcgcaggtc accatcggcc cggtgatcga caacggcttc
    12301 tattacgact tcgcctacga gcgcccgttc accccggaag acctgccggc gatcgaggcg
    12361 gagatgcaga agatcgtcaa ggaagcgctg ccgctgagcc gcagcgtgaa gtcgcgcgac
    12421 gaggcggtgg cctatttcaa gggcatcggc gaggcctaca aggccgagat catcgagagc
    12481 atccccgcga acgaggagtt gtcgctgtat tcgcagggcg agttcaccga cctctgccgc
    12541 ggcccgcacg tgccgggcac cgacaagctg cgcgcgttca agctgatgaa ggtggccggt
    12601 gcctactggc gcggcgacca caacaaccag atgctcagcc gcatctacgg caccgcctgg
    12661 ctgaacgaca aggacctcaa ggcctacctg acccagatcg aggaagccga gaagcgcgac
    12721 caccgcaaga tcggcaaggc gctggacctg ttccacctgc aggaggaagg cccgggcctg
    12781 gtgttctggc atccgaaggg ctggagcatc tggcagaccg tcgagcagta catgcgcaag
    12841 gtctaccgcg acaccggcta cggcgaggtg cgctgcccgc agatcctgga cgtgaccctc
    12901 tggcagaagt ccggccactg ggacaactac aaggacaaca tgttcttcac cgagtcggag
    12961 aagcgcacct atgcgctgaa gccgatgaac tgcccgggcc acgtgcaggt gttcaaccag
    13021 ggcctgcaca gctaccgcga cctgccgatc cgctacggcg agttcggcgc ctgccatcgc
    13081 aacgagccct ccggcgcgct gcacgggatc ctgcgcgtgc gcggcttcac ccaggacgac
    13141 ggccacgtgt tctgcaccga ggcgcagatc gagccggagg tgcgcgcgtt ccacgagcag
    13201 gccctgaagg tctactcgga cttcggcttt tccgacatcc agatcaagat cgcgctgcgc
    13261 ccggagtcgc gcctgggcga cgatgccacc tgggacaagg ccgaggacgc gctgcgcaat
    13321 gcactgcgcg cgtccggggt ggaatggcag gaactgccgg gcgagggcgc gttctacggc
    13381 ccgaagatcg agtaccacct gaaggacgcg atcggccgca cctggcagct gggcaccatg
    13441 caggtcgatt tcatgatgcc gggccggctg ggcgcggaat acgtggacga acacagccag
    13501 cgccgccatc cggtgatgct gcaccgcgcg atcgtgggct cgatggagcg tttcatcggg
    13561 atcctgatcg aacaccatgc tggccatttc ccggcctggc tggcaccggt gcaggcggtg
    13621 ctcatgaaca ttaccgatgc ccaggccgat tgggtcgaag aggcccggaa aacccttgcg
    13681 aatcaaggct tccgcgtggt ttccgatttg cggaacgaga agatcggcta taagatccgc
    13741 gagcacacgc tgcagcgcgt gccgtacctg ctggtggtcg gggaccgcga gaaggaaaac
    13801 ggccagctgt cggtgcgcac gcggggtggg gaagacctcg gtaccatgtc cgttgccgac
    13861 ttcgccgcgc gtttgcgtca ggagcaggcc gcctaaccgc cggcccgttc gaatacggcc
    13921 tgcgattccg gaatcgcagg cccacccggc tccccggagc caccctttgg agatcgcaac
    13981 atcagtaccg cacccgattc aaagcagaac cgacggaacc tcgagatccg cgtcccgcgc
    14041 gtgcgcgtga tcggcagcga cggcgagatg atcggcgtgc tgtcgcgcga cgaagcgctg
    14101 cgcatggccg aagaggaagc gctcgacctg gtcgagatcc agccgaacgc ggatccgccg
    14161 gtctgcaaga tcatggactt cggcaagttc aagttcgaac tgcagaagaa ggccaacgag
    14221 gccaagaaga agaccaagca ggtcgagatc aaggaagtga agttccgccc ggtcacggac
    14281 gagggcgact accagatcaa gctgcgcaag atgcgcgaat tcctgatcga aggcgacaag
    14341 atcaaggtca acatccgctt ccgtggccgc gagatgagcc accaggaact gggccg
//
LOCUS       DAORUK010000050        14270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_4109_length_14270_cov_19.043616, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000050 DAORUK010000000
VERSION     DAORUK010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 14270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14270
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4109_length_14270_cov_19.043616"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="PK818_05020"
     CDS             complement(<1..1159)
                     /locus_tag="PK818_05020"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HQY82130.1"
                     /translation="MKAQLRALIEQGLTALRAAGTLPADLASPDFSVERPKERAHGDF
                     STNAAMLLAKAARTNPRALAQQLLEAMPASDAIAGTELAGPGFINFHLTAAAWQQQVR
                     DIHASGAAFGRNASGGGKTVGVEYVSANPTGPLHVGHGRAGVIGDCIARVLAASGWNV
                     KREFYYNDAGVQIENLARSTQARAQGLKPGDDAWPAEAYNGDYIGDVAAAYLRGESVE
                     VEGHVVTGKDDAGDLDAIRKFAVAWLRREQNADLAAFGVSFDRYFLESSLYADGKVEE
                     TVRELVAHGHTYEEGGALWLRTTDFGDDKDRVMRKSDGTYTYFVPDVAYHLSKWQRGY
                     QRAVTELGADHHGSLARVRAGLQALDCGIPAGYPDYVLHQMVTVMRGGEEVK"
     gene            complement(1331..2005)
                     /gene="radC"
                     /locus_tag="PK818_05025"
     CDS             complement(1331..2005)
                     /gene="radC"
                     /locus_tag="PK818_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HQY82131.1"
                     /translation="MHIRDWPEHERPREKLLARGAGALSDAELLALFLGSGTAGRDAV
                     ATARALLAAHGPLRALLDRSARDLTRLDGIGSARACALAAALELGHRHLASQLQRGEV
                     LSDPTAAGRYFTQRLRARPREVFAALFLDARHRALGFEELFQGSIDGAEVHPRVLVER
                     ALAQAASAVIVGHNHPSGNPEPSAADRAVTARLKQALALVDVRLLDHFVIGDGAPVSM
                     AARGLV"
     gene            2105..3321
                     /gene="coaBC"
                     /locus_tag="PK818_05030"
                     /pseudo
     CDS             2105..3321
                     /gene="coaBC"
                     /locus_tag="PK818_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007964144.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
     gene            3318..3779
                     /gene="dut"
                     /locus_tag="PK818_05035"
     CDS             3318..3779
                     /gene="dut"
                     /locus_tag="PK818_05035"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HQY82132.1"
                     /translation="MSAHVLQVKVLDARFGAQWELPAYATEHSAGLDLRAALEAPLTL
                     HPGDAALVPSGLSIHIADPALCAVLLPRSGLGHRHGIVLGNGTGLIDADYQGPLLIST
                     WNRGREAYTINPGDRIAQLVLLPIVRAMLQVVDTFEASARGAGGFGHTGAR"
     gene            3808..6153
                     /locus_tag="PK818_05040"
     CDS             3808..6153
                     /locus_tag="PK818_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HQY82133.1"
                     /translation="MSDGEKRKLQLDPAQAARGLPVLVGLLGLLGLWLAATGGLAMRD
                     DARTASLQGARDSAIVATRGRLQEHQRMLATRIAAAPVQAALKAGDRAMAARALAAGW
                     EGVQRSEVWPADMQALYAALPRSGYGSLAVAEAALAAGKPAARVVKTRGQQRLALAAP
                     VTDARGPALAYVEFPLARLTQGIEAAALASGTYLALRQGDRSVLERGEATLSDSAESL
                     AAKVPDSDLRIAAAVPDAVATPFGLDGMPALGIGVMLLVGAAALWFGLRPRLLSASGR
                     QHHEEDSAPTLSELQVALPTPVVNAPVAVTQGEAAPTPDKGPGYVPVVIDAGIFRAYD
                     IRGIVGMTLDEGVAELIGQAVGSLMAEKGFEDIVVGRDGRLSGSQMADGLIAGLRKAG
                     RNVIDIGMVPTPVVYFGCFHLRTGCGVAITGSHNPPNYNGFKIVVGGETLSGNAITDL
                     HARIADDRLFAAKAPGSLSARDISEDYIERIAGDIQTGRQLKVVVDAGNGVAGELGPR
                     VLEAIGAEVTPLFCDIDGTFPNHHPDPSDPHNLEALIQMVARLDADLGIAFDGDGDRL
                     GVVTRSGKNIYPDRLLMLFAADVLQRNPGAVIVYDVKCTGRLPAHVLRHGGSPLMWKT
                     GHSLIKAKMRETEAELAGEMSGHFFFAERWYGFDDGIYAAARLLEILDATESPPEEVF
                     DSLPDGVSTPEIKVELFDGDPHAFVEQFRAAATFNGGRMSTIDGVRVDWPDGWGLVRA
                     SNTTPVLVMRFDADSSGAIARIQKTFREQLLALKSDLVLPF"
     gene            complement(6160..6837)
                     /locus_tag="PK818_05045"
     CDS             complement(6160..6837)
                     /locus_tag="PK818_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016901956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82134.1"
                     /translation="MSHANSILAAAVALALLGSGPAVAQKKLYCWNENGKKVCGDALP
                     AEASDSARTEISASSGLRTGQVSRALTDAERSAAAVAADAARRQAEAAAMEQRRDSAM
                     VESYVTESDLRRAYGERTELLDETLKASTLGLSNLRLSLLSLLRQAADRELAGEPVPK
                     RLASSIRNQHDELLRQQQILASQRLDRAALDAELADALQRYRKLKAPPDQAEASRVAG
                     PASPAPG"
     gene            complement(6867..7529)
                     /gene="pyrE"
                     /locus_tag="PK818_05050"
     CDS             complement(6867..7529)
                     /gene="pyrE"
                     /locus_tag="PK818_05050"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HQY82135.1"
                     /translation="MDEDTGDYRTRFLQLALRAEALRFGEFTLKSGRLSPYFFNAGRF
                     DSGASLAALAACYADALEAAGIAFDLLFGPAYKGIPLATALACEYARRGRDLPLAFNR
                     KEAKAHGEGGSLVGAPLAGKRVLIVDDVITAGTAIREALAIIGEAGGVPAGIVIALDR
                     QERVREELHESAAQAVAAGHGIAVVAVAGLGDLLEFAGERVELVRHRDALLAYRARYG
                     IA"
     gene            7597..8400
                     /locus_tag="PK818_05055"
     CDS             7597..8400
                     /locus_tag="PK818_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQY82136.1"
                     /translation="MRILSFNANGLRSAASKGFFGWFAAQDVDVLCVQETKAQEHQLA
                     DAAFRPEGYEAWFRDAQKKGYSGVAIYSRRAPDEVRTALGWAPFDDEGRYIEARFGNL
                     SVVSFYIPSGSSGALRQGFKEEVMAWLKPILDGWLASGREYVLCGDWNIVRARNDIRN
                     WTGNQKNSGCLPHERAWLNGLIADDCHDGLVVPPAHGWKDAFRVARPGVVEYSWWSQR
                     GAARASNVGWRIDYQICTPGIASRIVDAAIFPEPKFSDHAPCLVEYANA"
     gene            8393..9724
                     /locus_tag="PK818_05060"
     CDS             8393..9724
                     /locus_tag="PK818_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY82137.1"
                     /translation="MPDAAASPDQPRSKPSVWQAFVQPAAWTMFFFGFASGLPFLLVA
                     GTLAYWLKDNGIDLKSITLIASAGLAYTLKFLWAPLVDRWVLPLFGRLGLRRGWILFA
                     LALVMVALLAMAQLTPDRLAPFVWVTLVAAFAGATLDIAVDAYRVEIAPRSAQGALLA
                     TYSLGYRIALIITGALALVLADHLPWPTVYRVMALVLLVPMLAVLFAREPAIQRERAH
                     NWAVGLRDGVVGPFADFFQRFGPRIALGLLAFILLAKMSDQSLGGGIMAPFYIDQGYT
                     KTEIGAISKVYGIWVGIAGVFLAGIAIARWGVRWPLLAGLVLGAISNLLYVWLEGANG
                     DLAMLTLVISGENLAQGFQGTTLAAFLTALVNQRFSATQFALFSSLVMLPGKLLGAVS
                     GGIVEQTGYGTYFWITALVGIPALLLFFWLLPRVEMGDDDAPAVAIDRTSS"
     gene            9862..10107
                     /locus_tag="PK818_05065"
     CDS             9862..10107
                     /locus_tag="PK818_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82138.1"
                     /translation="MTDLVLRDLAPDLTGRIRKLAELQGRSMHEVVAAVLDAGVHACE
                     VQLRKQLDLEEQAALKEAIAALEQVPDDAGFGLIGRL"
     gene            complement(10117..11244)
                     /locus_tag="PK818_05070"
     CDS             complement(10117..11244)
                     /locus_tag="PK818_05070"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HQY82139.1"
                     /translation="MSNPACSQFFLGLISGTSADGIDAALVRFEGDDAPLRAELVAAR
                     TLPWQPDVRARLVWLGQGGALESLDELGELDAQVGEAFAQAALAILAVAGIAPSQVAA
                     IGSHGQTVRHRPYAAHPFSLQIGDPARIVERTGITTIADFRRRDIAAGGHGAPLLPAL
                     HHALLHDPGEHRAVLNLGGIGNLTLLPASGAVRGFDTGPANALMDLWCHEHTGRPYDE
                     AGAWGATGTIDTALLERLLGDPWFAQPPPKSSGREHFQRAWLAPHLRNGMAAADVQAT
                     LRELTARTIADALRANQADTRRVLVCGGGVHNPALMDALRRAMPGVALASTAESGLDP
                     DHVEAMGFAWLARQTLQGKPGNLVTVTGAAGPRILGAIHPA"
     gene            complement(11286..12695)
                     /locus_tag="PK818_05075"
     CDS             complement(11286..12695)
                     /locus_tag="PK818_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQY82140.1"
                     /translation="MVLRGNLARARASAAQEAPTARLAQAFNGRWTRRHWMHASLFAT
                     IGALLAAIVPGFSTAMQAAPHVQRTTLSLALPQLRLQDGNADRWHSVTLKSGQTLSAV
                     FVELGIPYDQLEKVMQHPRIKPALRRLRPGTALSFNLPSDGSVRAMRIEAAPGIGDEP
                     VELEFDGDALRERAVPQEITTRTVVLSGKVGKSLFASARKAGLGSSQLNQLTDEMFKY
                     DIDFDSDLDENDRFSVVVDQTWKNGQLASTGPVLAATFTMDGQLKSAFRHLRDGKAEY
                     FTPDGRSLKRAFIRMPIPYARLSSGFGGRRHPVLGRMRMHKGVDYAAGSGTPIQAAGD
                     ARVEFVGRKGGYGNTVILNHGAGKTTLYAHMSRFARLRPGQHVAQGTTIGYVGSTGMS
                     TGPHLHYEFRVNGAHVNPLKMTLPPPAPLSGPALAAFKNETRRALDKIREVEDVIYMP
                     DEDTRVATAKDAPSRSKRG"
     gene            12896..14125
                     /gene="tyrS"
                     /locus_tag="PK818_05080"
     CDS             12896..14125
                     /gene="tyrS"
                     /locus_tag="PK818_05080"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HQY82141.1"
                     /translation="MTELQHALDLISRGSEEILKREELEARLRTSVQDGGRPLRIKAG
                     FDPTAPDLHIGHTVLLNKMRQFQDLGHQVIFLIGDFTGMIGDPTGKNVTRKPLTREDV
                     AANAQTYADQVFKVLDRERTEVRFNSEWFGAMDAAGMIKLAAQHTVARMLERDDFAKR
                     YAAQQPIAIHEFLYPLVQGYDSVALKADVELGGTDQKFNLLMGRGLQEHFGQPPQIVL
                     TMPLLEGLDGVNKMSKSLGNYIGINEPAIDIVTKAMKVGDALMWKWIDLLSFEIGVDE
                     AKVLRRDIEGGALNPRDVKLRLARELAARFHGDAEADKAVAGWHAAVRGEGDVSNLPL
                     HEILVPAGGLRIAALLVAAGLMPSNSEANRKLKERAVRVDGEVFEDVARVFTPGFEGV
                     IAVGKRNFARVHLRAAA"
BASE COUNT         2089 a         4832 c         5121 g         2228 t
ORIGIN      
        1 gcttcacttc ctcgccgccg cgcatcaccg tgaccatctg gtgcagcacg tagtccgggt
       61 agccggcggg gatgccgcag tccagcgcct gcaggccggc gcgcacccgc gccagcgagc
      121 cgtggtggtc ggcgccgagc tcggtgaccg cgcgctggta gccgcgctgc cacttgctga
      181 ggtgataggc cacgtccggc acgaaatagg tgtaggtgcc gtcggacttg cgcatcaccc
      241 ggtccttgtc gtcgccgaag tcggtggtgc gcagccacag cgcgccgcct tcctcgtagg
      301 tgtggccgtg ggcgaccagc tcgcgcacgg tctcctccac cttgccatcg gcgtacagcg
      361 acgattccag gaagtaacga tcgaacgaca cgccgaacgc ggcgaggtcg gcattctgtt
      421 cgcggcgcag ccaggccacg gcgaacttgc ggatggcgtc gaggtcgccg gcatcgtcct
      481 tgccggtgac cacgtggccc tcgacttcga cgctctcgcc gcgcaggtag gccgcggcca
      541 cgtcgccgat gtagtcgccg ttgtaggcct ccgccggcca ggcgtcgtcg cccggcttca
      601 ggccctgtgc gcgcgcctgc gtcgagcgcg caaggttctc gatctgcacg ccggcgtcgt
      661 tgtagtagaa ctcgcgcttc acgttccagc cgctggcggc cagcacccgt gcgatgcagt
      721 cgccgatgac cccggcgcgg ccatggccga catgcagcgg gccggtcggg ttggccgaca
      781 cgtattccac gcccacggtc ttgccgccgc cgctggcgtt gcggccgaag gcggcgcccg
      841 aggcgtggat gtcgcgtacc tgctgctgcc aggcggcggc ggtcaggtgg aaattgatga
      901 agccggggcc ggcaagctcg gtcccggcga tggcgtccga tgccggcata gcctccagca
      961 attgctgggc cagcgcgcgc ggattggtcc tggcggcctt ggccagcagc atcgccgcgt
     1021 tggtcgagaa atcgccgtgc gcacgctcct tcgggcgctc gacggaaaag tcgggcgagg
     1081 cgaggtcggc gggcagggtg ccggcggcac gcagggcggt caggccctgt tcgatcaggg
     1141 cgcggagctg ggctttcacg aaggctatgc aatgcgtttc aacggttgcg cattgtacgc
     1201 gacgtaatac gagggtccct ccgtcattca ggtcaggccc gtgggccggg gctgccgcaa
     1261 ccaagcgagt atcggggagc gacttgcggc agccccggcc cacgggcgtg accggcgggt
     1321 ggcacggacc tcacaccaag ccccgggcgg ccatcgacac cggcgcgccg tcgccgatca
     1381 cgaagtggtc gagcaggcgc acgtcgacca gcgccagcgc ctgtttcagg cgggcggtca
     1441 ccgcgcgatc ggcggcgctg ggttcgggat tgcccgaggg gtggttgtgg ccgacgatca
     1501 ccgccgaggc ggcctgcgcc agcgcgcgct cgaccagcac ccgcgggtgc acctcggcgc
     1561 cgtcgatgct gccctggaac agctcctcga agccgagcgc acggtggcgt gcatccagga
     1621 acagggcggc gaacacctcg cgcgggcgtg cgcgcaggcg ctgggtgaag tagcggccgg
     1681 cggccgtcgg gtcggacagc acctcgccgc gttgcagctg cgaggccagg tggcggtgcc
     1741 cgagttccag cgcggccgcc agcgcgcagg cgcgggcgct accgatgccg tccagccggg
     1801 tcaggtcgcg tgcgctgcgg tccagcagtg cacgcagggg gccgtgcgcg gccagcagcg
     1861 cgcgggcggt cgccaccgca tcgcggccgg cggtgccgga gccgaggaac agcgccagca
     1921 gttccgcgtc ggacagcgcg ccggcgccgc gggccagcag cttttcgcgg ggacgttcgt
     1981 gttcgggcca gtcgcggatg tgcatggccg caggatccgc ggtggcgggt cgccgcggca
     2041 tcggtcgaat ccctgccttc gggtaagctg gcggccggtt cagcgcgtca ggaaagtccg
     2101 ccgcatgcag gccccacacc atcgtccgga ttctcgccgc atcctgcttt gcgtctgcgg
     2161 cggcatcgcc gcctacaaga gcgccgagct ggtgcggcgc ctgcgcgacg ccggctgcga
     2221 ggtgaccgtg gcgatgaccg agaacgcgca gcggttcgtc ggcgcgcaga cgttccaggc
     2281 gctttcgcac cggccggtgc ggacctcgct gtgggacgcg catgcggaag cggcgatggg
     2341 ccacctggag cttgcgcgct gggcgcagca ggtggtgatt gcgccggcca ccgccaacac
     2401 catcgcgaaa ctcgcgcacg gcatggccga cgacctggtc tcgaccctgt gcctggcgac
     2461 caccgcgccg gtcgcgatcg cacccgcgat gaaccacgtg atgtggcggc atccggcgac
     2521 cgcggccaac atcgccaccc tgcgcgcgcg cggggtgcag gtgatcggcc ccgacgacgg
     2581 cccgctggcc gagggtgaat ccggcccggg gcggatgtct gggcctgccg cgatcgccgc
     2641 cgccgtgctc ggggccaccg catgagcgcg gcgctcgccg gccgccggct gctggtcagt
     2701 gccggcccga ccttcgagga catcgatccg gtccgttacc tgggcaaccg cagcagcggc
     2761 aagatggggt tcgcgattgc atcggtggcc gccgaacgcg gcgccgaggt gaccctggtc
     2821 gccggcccgg tgcacctcgc cacgcctgcc ggcgtgcatc gcatcgacgt gcgcagcgcg
     2881 gcgcagatgt tcgatgcggt gatgggcgcg ctgccttgcg atgcctacat cggcgcggcg
     2941 gcggtggccg acttcacccc gcgcgcgttc gccggcagca agttgaagaa ggtggcgggc
     3001 accgacaccc tgaccctgga actggtgaag acccgcgaca tcctcgccga agtcgcggca
     3061 catgcactgc ggccggcgct ggtggtcggc ttcgccgcgg aaaccgatga tgtggcgacc
     3121 tatgcgcgcg gcaagctcga gcgcaagcgc gtcgacctgg tctgcgcgaa ccgggtcggc
     3181 gtgggtgcca ccggcttcga gagcgacgac aacgccctgc tggtgctggg cccgggcggc
     3241 ttcgagcgtg cgctggggcc ggccgccaag accgaacttg cggcgcgcct gctggatctg
     3301 ctcgcggagc ggctggcgtg agcgcgcacg tgctgcaggt gaaggtgctg gatgcgcgct
     3361 tcggtgcgca gtgggagctg ccggcgtacg ccaccgaaca cagcgcgggc ctggacctgc
     3421 gcgccgcgct ggaggcgccg ctgaccctgc accccgggga tgccgcgctg gtgccgtcag
     3481 ggctgtccat ccacatcgcc gacccggcgc tgtgcgcggt gctgttgccg cgctccgggc
     3541 tcggccacag gcacggcatc gtgcttggca acggcaccgg cctgatcgac gccgactacc
     3601 aggggccgct gctgatcagc acctggaacc gtggccgcga ggcctacacg atcaaccccg
     3661 gcgaccgcat cgcgcagctg gtgctgctgc cgatcgtgcg cgccatgctg caggtggtgg
     3721 atactttcga ggcaagcgca cgcggcgccg gcggcttcgg ccataccggc gcgcgctgac
     3781 gggcgcgcac tgactgaggg acaggcgatg agcgacggcg aaaagcgcaa gctgcaactc
     3841 gatcccgcgc aagcggccag gggcctgccg gtcctggtcg gcttgctcgg tttgctgggc
     3901 ctgtggctcg ccgcgaccgg cggcctggcc atgcgcgacg acgcgcgcac ggcatcgctg
     3961 cagggtgcgc gcgacagcgc catcgtggcg acccgtggcc ggctgcagga acaccagcgg
     4021 atgctggcga cgcggattgc cgcggccccg gtgcaggcgg cactgaaggc gggcgaccgt
     4081 gccatggccg cgcgcgcgct ggcggctggc tgggagggtg tacagcgcag cgaggtctgg
     4141 ccagcggaca tgcaggcgct gtatgccgcc ctgccgcgca gtgggtacgg cagcctggcc
     4201 gttgccgagg ccgcgctggc cgccggcaag ccggcggcac gcgtggtcaa gacccgcggc
     4261 cagcagcgcc tcgcgctggc ggcgccggtc accgatgcgc ggggaccggc actggcctac
     4321 gtggagtttc cgttggcgcg cctgacccag ggtatcgagg ccgcggcgct tgcctccggc
     4381 acctacctgg ccctgcgcca gggcgaccgc agcgtgctgg agcgcggcga ggccacgctg
     4441 tcggattctg ccgaatcgct ggcggccaag gtgcctgaca gcgacctgcg gatcgccgcg
     4501 gcggtgccgg atgcagtagc cacgccgttc gggctggatg ggatgccggc gctgggcatc
     4561 ggggtgatgc tcctggtcgg ggcggcggcg ttgtggttcg gcctgcgccc gaggctgctc
     4621 agtgcaagtg gccggcagca ccacgaggaa gacagtgccc cgaccctgtc ggaattgcag
     4681 gtggcgttgc ccactccggt cgtcaacgcg ccagtcgcgg tgacccaggg cgaggccgca
     4741 cccacgccag acaaggggcc gggctatgtc cccgtggtga tcgatgccgg aatcttccgc
     4801 gcatacgata tccggggcat cgtcggcatg accctcgacg aaggagtggc ggagctgatc
     4861 ggccaggcag tgggctcgct gatggccgag aagggattcg aggacatcgt ggtcggccgc
     4921 gatggccgcc tgtcgggttc gcagatggcg gacggcctca tcgccggttt gcgcaaggcc
     4981 gggcggaatg tcatcgacat tggcatggtg ccgacgcccg tggtctattt cggctgcttc
     5041 cacctgcgta ccggctgcgg tgtggccatc accggaagcc acaacccgcc caactacaac
     5101 ggcttcaaga tcgtggtcgg cggggaaacc ctgtccggca acgcgatcac cgacctgcac
     5161 gcgcggatcg ccgacgacag gctgttcgcc gcgaaggcgc cgggttcact aagcgcacgc
     5221 gacatcagcg aggactacat cgagcggatc gccggcgaca tccagaccgg gcgacagctg
     5281 aaggtggtcg tggatgcggg taatggcgtc gccggcgaac tcggtccgcg cgtgctggag
     5341 gcgattggcg ccgaggtcac cccgctgttc tgcgatatcg acggcacctt ccccaaccac
     5401 cacccggatc cgagcgatcc gcacaatctt gaagccttga tccagatggt ggcgcgactg
     5461 gatgctgacc tcggcatcgc cttcgatggc gatggtgacc ggcttggcgt ggtcactcgc
     5521 agcggcaaga acatctaccc cgatcgcctg ctcatgctgt tcgcggcgga cgtgctgcaa
     5581 cgcaacccgg gcgcggtgat cgtgtacgac gtgaagtgca ccggtcgcct gccggcgcac
     5641 gtcctgcgcc acggcggcag cccgctgatg tggaagaccg ggcattcgct gatcaaggcg
     5701 aagatgcgcg agaccgaagc ggagctggcc ggcgagatga gcgggcactt cttcttcgcc
     5761 gagcgctggt acgggttcga cgacggaatc tatgccgccg cgcgcctcct tgagatcctg
     5821 gatgcgaccg agagcccgcc cgaagaagtg ttcgattcgc tgccggacgg cgtttccacg
     5881 ccggagatca aggtcgagct tttcgatggc gacccgcacg cgttcgtcga gcaattccgt
     5941 gcggctgcca cgttcaatgg cggccggatg tcgaccatcg atggcgtacg ggtggattgg
     6001 ccggatggct gggggctggt gcgcgcttcc aacaccaccc cggtcctggt gatgcgcttc
     6061 gatgcggaca gcagtggggc gatcgcgcgc atccagaaga ctttccgcga acaactcctc
     6121 gcgttgaagt ccgatctcgt cctgcctttc tgaacctgct cagccgggag cgggcgaggc
     6181 cggaccggcc acgcgactcg cttcggcttg gtcgggaggt gccttgagct tgcgatagcg
     6241 ctgcagcgca tcggccagtt cagcgtcaag cgcggcgcgg tccagtcgtt gtgacgccag
     6301 tatctgttgc tgccgcagca actcgtcgtg ctggttgcgg atcgaactgg cgagccgctt
     6361 gggcaccggc tcccccgcca gttcacggtc ggcggcctgg cgcagcaggc tgagcaggct
     6421 gaggcgaagg ttggacaggc ccagcgtgct cgccttcagg gtttcgtcga gcagttcggt
     6481 gcgctcgcca taggcgcggc gcaggtcgga ctcggtcacg tatgactcga ccatcgccga
     6541 gtcgcggcgt tgctccatcg cagcggcctc ggcctggcgc cgcgcggcat cggcggcgac
     6601 cgcggcggcg ctgcgttcgg catcggtcag cgcgcgcgac acctggccgg tgcgcaagcc
     6661 gctgctggcg ctgatctcgg tgcgcgcgct gtccgaggct tcggcgggca gggcatcccc
     6721 gcacaccttc ttgccgttct cgttccagca atacagcttc ttctgcgcca ccgcagggcc
     6781 gctgcccagc agtgcaagcg cgaccgcggc cgcgaggatc gaattggcgt gcgacatgac
     6841 ggccccctgt ggcggtcgtg aagcggtcat gcgatgccgt agcgcgcgcg gtaggcgagc
     6901 agggcgtcgc ggtggcgcac aagttccacc ctttcgccgg cgaattcaag caggtcgccg
     6961 aggcccgcca ccgccaccac cgcgatgccg tgtccggccg ccaccgcctg cgcggccgat
     7021 tcgtgcaact cctcgcgcac gcgctcctgg cggtcgaggg cgatgacgat gccggccggc
     7081 acgccgccgg cctcgccgat gatcgccagc gcctcgcgga tcgcggtgcc ggcggtgatc
     7141 acgtcgtcca cgatcagcac ccgcttgccg gccagcggcg cgccgaccag gctgccgccc
     7201 tcgccgtgcg ccttggcttc cttgcgattg aacgccagcg gcaggtcgcg cccgcggcgg
     7261 gcgtattcgc aggccagcgc cgtggccagc gggatgccct tgtaggccgg gccgaacagc
     7321 aggtcgaagg cgatccccgc ggcctcgagc gcgtcggcat agcaagccgc cagcgcggcg
     7381 agcgaggcac ccgaatcgaa gcgtccggca ttgaagaagt acgggctgag gcggcccgac
     7441 ttgagggtga attcgccgaa gcgcagggcc tccgcgcgca acgccagctg caggaaacgg
     7501 gtgcggtagt cgccggtgtc ttcgtccatg ccgctagtgt ccacgatcgc ggtggttttc
     7561 ggctacgctc ccgcatcgcc cgctccccgg tcctgcatgc gcatcctcag cttcaacgcc
     7621 aacggcctgc gatcggccgc cagcaagggg ttcttcggct ggttcgcggc gcaggacgtg
     7681 gacgtgctgt gcgtgcagga aaccaaggcg caggagcacc agctggccga cgccgcattc
     7741 cggcccgaag gctacgaggc atggttcagg gacgcgcaga agaagggcta cagcggcgtg
     7801 gcgatctaca gccggcgcgc gcccgacgag gtgcgcacgg cactgggctg ggcgccgttc
     7861 gacgacgagg gccgctacat cgaggcgcgc ttcggcaacc tgtcggtggt cagcttctac
     7921 attccttccg gaagctccgg cgcgctgcgc cagggcttca aggaagaggt gatggcctgg
     7981 ctcaagccga tcctggacgg ctggctggcc agcggccgcg agtacgtgct gtgcggcgac
     8041 tggaacatcg tccgcgccag gaacgacatc cggaactgga ccggcaacca gaagaactcc
     8101 ggctgcctgc cgcacgagcg cgcctggctc aacgggctga tcgccgacga ctgccatgac
     8161 ggcctggtgg tgccgcccgc gcacggctgg aaggacgcat tccgcgtcgc caggcccggc
     8221 gtggtcgaat attcgtggtg gtcgcaacgc ggcgcggcgc gcgccagcaa cgtcggttgg
     8281 cggatcgact accagatctg cacgcccggc atcgccagcc ggatcgtgga tgccgcgatc
     8341 ttcccggagc cgaaattctc cgaccacgcg ccctgcctgg tggagtacgc caatgcctga
     8401 tgccgccgct tcgcccgacc agccccgcag caagccgtcg gtgtggcagg ccttcgtcca
     8461 gccggcggcg tggacgatgt tcttcttcgg cttcgcctcg gggctgccgt tcctgctggt
     8521 cgcgggcacg ctggcgtact ggctgaagga caacgggatc gacctcaaga gcatcaccct
     8581 gatcgcgagc gccgggctcg cctacacgct gaagttcctg tgggcgccgc tggtcgatcg
     8641 ctgggtgttg ccgctgttcg gccggcttgg cctgcggcgc ggctggatcc tgttcgcgct
     8701 ggcgctggtg atggtcgcgc tgctggcgat ggcgcagctc accccggacc ggctggcgcc
     8761 gttcgtgtgg gtgaccctgg tcgctgcgtt cgcgggcgcc accctcgaca tcgcggtcga
     8821 cgcgtaccgg gtcgagatcg ccccgcgcag cgcacagggc gccctgctcg ccacctattc
     8881 gctgggctac cggatcgcgc tgatcatcac cggtgcgctg gcgctggtgc tggcggacca
     8941 cctgccgtgg cccacggtgt accgggtgat ggcgctggtg ctgctggtgc cgatgctggc
     9001 ggtgctgttc gcgcgcgagc cggcgatcca gcgcgagcgt gcgcacaact gggcggttgg
     9061 cctgcgcgac ggcgtggtcg ggccgttcgc cgacttcttc cagcgcttcg gcccgcgcat
     9121 cgcgctgggg ctgctggcct tcatcctgct tgcgaagatg tccgaccagt cgctcggcgg
     9181 cggcatcatg gccccgttct acatcgacca gggctacacc aagaccgaga tcggcgcgat
     9241 cagcaaggtc tacggcatct gggtcgggat cgccggcgtg ttcctggcgg ggatcgcgat
     9301 cgcgcgctgg ggcgtgcgct ggccgctgct ggcggggctg gtgctgggcg ccatcagcaa
     9361 cctgctgtac gtctggctgg aaggcgccaa cggcgacctg gcgatgctga cgctggtgat
     9421 ctcgggtgag aacctggcgc agggcttcca gggcaccacc ctggccgcgt tcctcaccgc
     9481 gctggtcaac cagcggtttt cggcgaccca gttcgcgctg ttctcatcgc tggtgatgct
     9541 gcccggcaag ctgctgggcg cggtgtccgg cggcatcgtc gagcagaccg gctacggcac
     9601 ctatttctgg atcaccgcgc tggtcgggat cccggcgctg ctgctgttct tctggctgct
     9661 gccgcgggtg gagatgggcg atgacgatgc accggcggtg gcgatcgacc gcacgtcgtc
     9721 gtgagcgtgt cgccgcgcac cgacgcgtgg ctggcgcaat gcgcgcaagc cggcggacgc
     9781 cgcggccctg cgcttgccct gcgcgccgca accgggtaga taggaacggc gtttcccccg
     9841 acgccacacg gaagtcgtgc gatgaccgat cttgtcctgc gtgatctcgc cccagacctg
     9901 accggccgca tccgcaagct ggccgagttg cagggccgca gcatgcatga ggtggtggcg
     9961 gcggtgctgg acgcaggcgt ccacgcctgc gaggtgcagc tgcgcaagca gctcgacctc
    10021 gaggaacagg ccgccctgaa ggaggcgatc gccgcgctcg aacaggtgcc ggacgatgcc
    10081 ggcttcggcc tgatcgggag actctgagcg gccgcctcag gccggatgga tcgcgcccag
    10141 gatccgtggt ccggccgcgc cggtgacggt cacaaggttg ccgggcttgc cctgcagggt
    10201 ctggcgcgcc agccacgcga atcccatcgc ctccacgtga tccggatcca gcccggattc
    10261 cgccgtcgac gccagcgcca cccccggcat cgcccggcgc agggcatcca tgagcgccgg
    10321 gttgtgcacg ccgccaccgc acaccagtac ccgccgggtg tccgcctggt tcgcgcgcag
    10381 ggcgtcggca atggtccgcg cggtcagttc gcgcaaggtc gcctgcacgt ccgccgcggc
    10441 catgccgttg cgcaggtgcg gggccagcca cgcgcgctgg aagtgctcgc ggccgctgct
    10501 cttgggtggc ggctgcgcga accaggggtc gcccagcagg cgttccagca gcgccgtgtc
    10561 gatcgtaccg gtggcgcccc aggcgccggc ctcgtcgtag gggcggccgg tgtgttcgtg
    10621 gcaccacagg tccatcagcg cattcgcggg cccggtgtcg aacccgcgca cggcgccgct
    10681 ggccggcagc agggtcaggt tgccgatccc gcccaggttg agcaccgccc gatgctcgcc
    10741 tggatcgtgc agcagcgcat ggtgcagggc cggcaacagc ggcgcgccat ggccgccggc
    10801 ggcgatgtcg cggcggcgga agtcggcgat ggtggtgatg ccggtgcgct cgacgatccg
    10861 ggccgggtcg ccgatctgca gcgaaaacgg gtgcgccgcg taggggcggt ggcggacagt
    10921 ctgtccatgc gaaccgatcg cggcgacctg cgacggtgcg atcccggcga ccgccaggat
    10981 cgccagcgca gcctgcgcga atgcctcgcc cacttgcgca tccagctcgc cgagttcatc
    11041 cagcgactcc agcgcgccgc cctgcccgag ccagaccagc cgcgccctta cgtccggttg
    11101 ccagggcagg gtgcgtgccg ccaccagttc cgcccgcagc ggggcgtcgt cgccttcgaa
    11161 gcgcaccagc gcggcatcga tgccgtcggc gctggtgccg gaaatcaggc cgaggaagaa
    11221 ctggctgcag gcggggttcg acatgcgcgg aagtatcgca tcgcgcgatt ccgccggtgg
    11281 cgcgttcagc cgcgcttgct gcggctgggc gcgtccttcg cggtggccac ccgggtgtcc
    11341 tcgtcgggca tgtagatcac gtcctcgacc tcgcggatct tgtcgagcgc gcggcgggtc
    11401 tcgttcttga acgcggcaag cgccgggccg ctcaacggcg ccggcggcgg cagggtcatc
    11461 ttcagcggat tgacgtgcgc accgttgacc cggaattcgt agtgcaggtg cgggccggtc
    11521 gacatcccgg tgctgccgac gtagccgatg gtcgtgccct gcgccacgtg ctggcccggg
    11581 cgcagcctgg cgaagcgcga catgtgcgcg tacagcgtgg tcttgccggc accgtggttg
    11641 aggatcacgg tgttgccgta gccgcccttg cggcccacga actcgacccg tgcatcgccg
    11701 gcggcctgga tcggggtacc gctgccggcg gcatagtcca cgcccttgtg catgcgcatg
    11761 cggcccagca ccgggtgccg gcgcccgccg aaacccgagc tcaggcgcgc atacgggatc
    11821 ggcatgcgga tgaaggcgcg cttcagcgag cgaccatccg gggtgaagta ttcggccttg
    11881 ccgtcgcgaa ggtgccggaa cgcgctcttc agctggccgt ccatggtgaa ggtcgccgcc
    11941 agcaccgggc cggtgctcgc cagctggccg ttcttccagg tctggtccac caccacgctg
    12001 aagcggtcgt tttcgtcgag gtcggaatcg aagtcgatgt cgtacttgaa catctcgtcg
    12061 gtcagctggt tcagctgcga actgcccagc ccggccttgc gcgcggaggc aaacagcgac
    12121 ttgccgacct ttccgctcag caccacggta cgggtggtga tctcctgcgg caccgcgcgc
    12181 tcgcgcaacg catcaccgtc gaattccagc tccaccggct cgtcgccgat gcccggtgcc
    12241 gcctcgatcc gcatcgcgcg cacgctgcca tcggacggca ggttgaagct cagcgcggtg
    12301 cccgggcgca accgccgcag cgccggcttg atccgcgggt gctgcatgac cttctcgagc
    12361 tggtcatagg gaatgccgag ttcgacgaac accgcgctca gggtctgccc cgacttcagc
    12421 gtcaccgagt gccagcggtc ggcgttgccg tcctgcaggc gcaactgcgg caacgccagc
    12481 gaaagggtgg tgcgctgcac atgcggcgcg gcctgcatgg cggtggagaa gccgggcacg
    12541 atcgccgcga gcagcgcgcc gatggtcgcg aacaggctgg catgcatcca gtgccggcgc
    12601 gtccagcggc cattgaaggc ctgcgccagc ctggcggtgg gcgcttcctg cgcggcggag
    12661 gccctggcac gggcgaggtt tccgcgaagc accacggcgt gcgtggaagg cgtcgtcgga
    12721 tccgtcatgt ggctgttccc cgctgtgacg aaggcgtcaa ggcggtacca tagtcatgcg
    12781 aaaaacatgc gtcaactcat tgaatccccg aatgtttttc gattccgaca tttaacgtta
    12841 ctttaacgaa cctttaccca atgcggtcac agtccggccg ccagcgagac ttccagtgac
    12901 cgaactgcag cacgccctcg atctcatctc ccgcggcagc gaggaaatcc tcaagcgcga
    12961 ggaactggag gcgcgcttgc gcacgtccgt ccaggatggc ggccgcccgc tgcggatcaa
    13021 ggccggtttc gacccgaccg cgcccgacct gcacatcggc cacacggtgc tgctgaacaa
    13081 gatgcggcag ttccaggacc tcggccacca ggtgatcttc ctgatcggcg acttcaccgg
    13141 gatgatcggc gaccccaccg gcaagaacgt cacccgcaag ccgctgaccc gcgaggacgt
    13201 ggcggccaat gcgcagacct acgccgacca ggtcttcaag gtgctcgacc gcgaacgcac
    13261 cgaggtgcgc ttcaacagcg aatggttcgg cgcgatggat gccgcaggca tgatcaagct
    13321 cgcggcgcag cacacggttg cgcgcatgct ggagcgcgac gacttcgcca agcgctacgc
    13381 cgcgcagcag ccgatcgcca tccacgagtt cctgtatccg ctggtgcagg gctacgactc
    13441 ggtggcgctg aaggcggacg tcgaactcgg cggcaccgac cagaaattca acctgctgat
    13501 ggggcgcggc ttgcaggagc acttcgggca gccgccgcag atcgtgctga cgatgccgtt
    13561 gctggaggga ttggacggcg tcaacaagat gagcaagtcg ctcggcaact acatcggcat
    13621 caacgaaccg gcgatcgaca tcgtcaccaa ggcgatgaag gtcggtgacg cgttgatgtg
    13681 gaagtggatc gacctgctca gcttcgagat cggggtcgac gaggcgaagg tgttgcggcg
    13741 cgacatcgaa ggcggcgcac tgaatccgcg cgacgtgaag ttgcggctgg cgcgtgaact
    13801 cgccgcgcgc ttccacggcg acgccgaggc ggacaaggcg gtggcgggct ggcacgccgc
    13861 ggtgcgcggg gagggcgatg tgtcgaacct gcccctgcat gagatcctgg tgcccgccgg
    13921 gggccttcgc atcgccgcgc tgctggtcgc ggcgggcctg atgccgagca attcggaggc
    13981 caaccgcaag ctcaaggagc gggcggtgcg cgtcgatggc gaggtgttcg aggacgtcgc
    14041 gcgggtgttc acgcctggct tcgagggcgt gattgcagtc ggaaagcgca atttcgccag
    14101 ggtccatctc cgcgcagctg cgtgaaaccc cggttggcgc attcagctct tgcggccccg
    14161 aaccaactcc cctagaatga tcgacccggt acgaacaaca tcacgttgaa cgcatcggaa
    14221 gcaccaaaag cccacggcaa ggggtgttga cggacgtcaa aaaccttgta
//
LOCUS       DAORUK010000051        13851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_4292_length_13851_cov_20.133227, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000051 DAORUK010000000
VERSION     DAORUK010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 13851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13851
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4292_length_13851_cov_20.133227"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PK818_05085"
     CDS             <1..537
                     /locus_tag="PK818_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HQY82142.1"
                     /translation="VTQLLARARAGDAQALGAAYSAVYEELKHAARSQLRRMRDAFQT
                     TALVHEAYLKLAGGAPLAALDRNHLLALSARAMRQVLVDDARARKADKRGGGQEALTL
                     TASLGNGDAAAVEVLALDELLASLHRVDERAAQIVELRYFGGYSETEIAAMLGISDRT
                     LRRDWRRARAFLLAELGT"
     gene            534..3416
                     /locus_tag="PK818_05090"
     CDS             534..3416
                     /locus_tag="PK818_05090"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQY82143.1"
                     /translation="MSDAATERRRRALAIFDIVADLAGEARLQQIDALCDGDQALREQ
                     VQALLDADAGTREPFSGDVAAWGQALQAQPADGASDPMLGRSIGAWRVREVIGRGGMG
                     AVYAVERSDGAYTQQAALKLIRTGADSPAARERFLRERQILAGLQHPNIATLLDGGIS
                     TDGEPYFVMERVDGEPIDRWCDARGLGLRERVVLFLQVLDAVRFAHRNLVVHRDLKPS
                     NLLVDGDGRVKLLDFGIAKQLEGADATATHDRALTFEYASPEQLHDAPITTATDLWQL
                     GVVLHRLLAGAHPFGLTRDTPVARQLQQLEREPEPLTRAAAQASTEQAAQRGGHTPAT
                     LARALRGNLAAIVQACLRRDPDTRYASADALANDLRAWLDDRPIAAVRLSRGERSRLW
                     LRRNRGLAAAVAAVALALLAGTGLAMWQAREAREQARIAERESESARAALAFLTDTLA
                     AAAPERAMSTEVSVRQLLDKARQQLDQRTLAPQVRQSIQRMLGHLYGSLGEAETSARL
                     FAAGLQGVQPRNRDDALALARDLDGQSGVLAVLERGKASLASARLGVDLRQRFAPDDP
                     RQRFLGQRQLAVAYTSLADCKQGDAQWKQALATATSMRDPTVDEIGEAHVAYARMLFN
                     CGEYARGLQVSADGLGFVDGRRLPALSPLRANLLWVKAMNGHAIGKLSVADTEALLRQ
                     AIAIHEKTIGAGGAQLGEFHSTLGTVLQTSGRLPEALVAHERSAELSRAAGARPIDQA
                     ITQYNIGYLYRDYGDYEKSLAAHRRSLAIFDAAGIDRDHIERRRAEKWYALAQIQSGR
                     AADAREQLLRLQQRARALDGEESVEYLDVVWQLLRAAVVMGDRANGEPLLADARARAA
                     RFVPPAHPVFTEFLRDEAQFARLQGDLAGAERLQREALKRSDAIGNPTEPSIVRVELA
                     DLLVQRGQDAEARILLLQALPVLRKYMHARHATRARSEVLARRLGV"
     gene            complement(3448..4980)
                     /locus_tag="PK818_05095"
     CDS             complement(3448..4980)
                     /locus_tag="PK818_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82144.1"
                     /translation="MDRVLAEGPEPTLQKLRPVDYWLHYKLMQATGVERELGGEAQAI
                     AALQALGDAYERKLRGAEADLPKLVPAAFTGEGMDSGFTGMGLGGFVGLMTGGMLSGA
                     VSSMSDKDLAELVKQGPIKFGRNDGNSAVEFGKDGSLSQSMEFEVNENGLNGKVRIKT
                     RMDACPDPQGRVSVDIDVDSQMSVSGKPGTGGYVRSNFKYERYLDDDAHLIDTAEGGA
                     SNLRISMGGSENFQSQSVDITTGRERGGKPIFEHHGEQGFSIFRPGEVERTRALLQGA
                     ETLQTLIAEVMLRGLGASAGSPWESGRCIDLQVTSSPGKRKGIKPNTAFDIDARPRAK
                     ADGAAAGGTVAATLSGGASLQPASGKVRADAKYQYAGPAKKREKASIDFESRSKRGVG
                     KASLGFDTNEGAYRVKGGQNDFVANTVVCSLDGPFDIRSTAGIVMHMSGGEGGGSWTQ
                     SGNAAGVAWSGGGSYTLALDGDGSGRIEARGSSSIATPMGRFSDSVEPVFSVTRVDQA
                     CD"
     gene            complement(5215..6498)
                     /locus_tag="PK818_05100"
     CDS             complement(5215..6498)
                     /locus_tag="PK818_05100"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HQY82145.1"
                     /translation="MKSMHTRARIAAAIFVAIALVPACKRDADTPAANPNGDYRVQVW
                     ALPAAAGSMAPDLSLASGDRLLLSWLNRQQGRRTALQFATYTDAGGWQSQPRTIAVGQ
                     SLVANGADTPHLLGTPDGAIWVQWLQARPDSPSGYDTVLARSRDGGMSWNQITNVNDD
                     GLGVEHGFATLWAAGSDRIGIAWLDGRAQAAAHAEGAHAGEGDGTGAMQLRANAFDMD
                     LGRGTDMVADARTCDCCQTDVALTGKGPLLAYRDRADDEVRDIAVLRFEAGAWSAPKA
                     VHADGWKTEACPVNGPAVAARGNTAVVGWYSEGGGIAAVRIARSTDAGDAFAAPVIVD
                     KGAAVLGRVDVALDAQQAWVTWLREDASGQTLMLARYAPDLSRELQRMVVARLDARGH
                     ASGTPKLVANASGAWLAWTDSIDGVAALKGALVAR"
     gene            complement(6538..8427)
                     /gene="mnmG"
                     /locus_tag="PK818_05105"
     CDS             complement(6538..8427)
                     /gene="mnmG"
                     /locus_tag="PK818_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HQY82146.1"
                     /translation="MNDSFYSFDVIVIGGGHAGTEAALASARAGARTLLLTHSIDTVG
                     AMSCNPAIGGIGKGHLVKEIDALGGIMAKAADAAGIQWRTLNASKGPAVRATRCQADR
                     SLYRSFIRRAVETQPNLTVFQAAVDDLVIEQDAVRGAITNTGLRFHAPAVVLTAGTFL
                     AGKIHIGETQYAAGRMGDPPSTALAHKLREGRFVVDRLKTGTPPRIDGRTLDYAVMTE
                     QPGDDPRPVMSFMGSHDDQPAQVSCWITDTSEHTHQIIRDALHRSPLYSGQIEGIGPR
                     YCPSIEDKVVRFAEKASHQIFVEPEGLTVSEIYPNGISTSLPFDVQLALVRSIRGFEQ
                     AHITRPGYAIEYDFFDPRGLKASLETKAVAGLFFAGQINGTTGYEEAAAQGLLAGTNA
                     ARFVHGKEAWSLRRDEAYLGVLVDDLITHGTSEPYRMFTSRAEYRLQLREDNADARLT
                     PTGRELGLVDDARWERFERKREAVIAETARLSALWATPNNALGAALARSIGIEVSREG
                     NAIDLLRRPELDYATLMQVPELGPGVEDAKVAEQVEIETKYAGYLDRQREEIARQRRH
                     EATAIPDAFDYAGVRGLSAEVLQKLQRVRPQTVGQAQRIPGMTPAAISLLLVHLERQR
                     RERAA"
     gene            complement(8823..9443)
                     /locus_tag="PK818_05110"
     CDS             complement(8823..9443)
                     /locus_tag="PK818_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82147.1"
                     /translation="MFRRILLSMLATATLAGCATAYTYRGGNGDYYYGQPQTEYRYHD
                     PYGFYGGFGYGGYAPGVHYDRYGRVIYGSYYGYFGYPPGYGSPWWYRPRAPHDGHDHG
                     GDGHDGDGHGGEDGDGSDRRPPWRNPGGLSAPGGQAMEDRDDTRPRARRMQAGSTPSM
                     LPAQQREQREQRTTAPPPPRMRSDDGGGSRMGRAIRNAKASPVPEE"
     gene            9563..10549
                     /locus_tag="PK818_05115"
     CDS             9563..10549
                     /locus_tag="PK818_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQY82148.1"
                     /translation="MTPTAPMPNFTDVLAAAARIAGRAHATPVITSRALDAHAGAGLL
                     FKCEQLQRIGAFKFRGACNAVMSLDDTTATRGVVTHSSGNHGAALALAAQDRGIPCHV
                     VVPDGAVRAKLAAIEHYGAILHRCAATIAAREAMCAKVQADTGATTVHPYTDPRVIAG
                     QGTAALELLTAHPDLEALVVPVGGGGLAAGTALAVRALAPDCRLYLAEPSGAADTARS
                     FARGTLDHDMVPDTICDGLRGTLGAINFEILRNAGAQVLTVDDTQAVAAMRLLWTRTK
                     QLLEPSSATVLAAVLANPALFARRRVGIILSGGNVDLDALPALFALAGDAQP"
     gene            10546..10971
                     /locus_tag="PK818_05120"
     CDS             10546..10971
                     /locus_tag="PK818_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketosteroid isomerase-related protein"
                     /protein_id="HQY82149.1"
                     /translation="MKIDGTRVHDRATELVLAYYAAFNRGDWAAMQALLADDVVHDLN
                     QGPREAGKPAFAAFQQRMARCYREQLRDVVVLASPDGLRAAAEYVVHGEYLQADEGLP
                     PAHGQQYALPGGAFFEVRDGLIARVSNYYNLQDWLAQVR"
     gene            complement(10985..11908)
                     /gene="bioC"
                     /locus_tag="PK818_05125"
     CDS             complement(10985..11908)
                     /gene="bioC"
                     /locus_tag="PK818_05125"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HQY82150.1"
                     /translation="MSARLFDAKQVRRAFSRSAAGYDAAARLQHAVEARLLESLDYLD
                     DPALKREAPRRVLDLGCGTGRASLAMQARWPKAQVVSLDLALPMLQQARVASGRPAGA
                     WLSNPFARTPVPVCADARALPLAEASVDVLFSNLCLQWVEDLDALFAGFRRVLKPHGL
                     LLVSTFGPETLWELREAFARADAAPHVSPFADIAGFGDALVRAGFHQPVLDREELTTH
                     YPDLASLMRELRAIGATNALASRRHTLTGRARFTVAADAYETHRDGAGLPATWETITA
                     MAWAPEAGTPLREGDVEVASIPLSRIPIRRR"
     gene            complement(11905..12717)
                     /gene="bioH"
                     /locus_tag="PK818_05130"
     CDS             complement(11905..12717)
                     /gene="bioH"
                     /locus_tag="PK818_05130"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HQY82151.1"
                     /translation="MHVDVAGSGPPLVLLHGWAMHGGIFAPLVQALRARRTLHVVDLP
                     GHGRSRASGLPLALDACADAVLDAVPDAPWCGWSLGGLVALHAASKQPARIHALAMVC
                     ATPKFVVAGDWPQGMPAEVFHGFADGLQADWRASVDRFIALEAFGSDHMREELRMLRD
                     AVLAHGEPSPRVLAEGLRLLEGSDLRAALPALRMPSLWIGGRRDRLVNPLAMQAAAAL
                     APDARFLQVEHAGHAPFLTHADAVADALLDFLASCDPRPNPATHAEAGAAQP"
     gene            complement(12720..>13851)
                     /gene="bioF"
                     /locus_tag="PK818_05135"
     CDS             complement(12720..>13851)
                     /gene="bioF"
                     /locus_tag="PK818_05135"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009595552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HQY82152.1"
                     /translation="REAAHRRRHLRTVTRRAGVVCTVDGRDLLNFCSNDYLGLSQHPR
                     VIAALRDAAGEGSGAGASHLVCGHGAQHAALEAELADWLQAPRALLFGSGYLANLAVV
                     QALLGAGDLCVQDKRNHASLIDGARLAGCAFKRYPHGDAEGAARQLASQAEGAALLAS
                     DGVFSMDGDVAPLRALAQVARAQRATLYIDDAHGVGVLGDAGRGSIDAAGLAAADVPL
                     RLVTFGKALGSAGAAVVGDADLVAHLAETARPHIYTTALPPAQAAATREAVRIARAGD
                     DLRARLAANIGRLQAGARARGLALLPSDTPIQPLLVGGDADALALAAALEQQGYWVAP
                     IRPPTVPEGSARLRITLAAGHLPAQVDGLLDALARARDAVAA"
BASE COUNT         1971 a         4997 c         4844 g         2039 t
ORIGIN      
        1 gtcacccagt tgctcgcgcg cgcgcgcgcc ggcgatgcgc aggcgctggg cgccgcctac
       61 tccgcggtgt acgaggaact caagcacgcc gcgcgcagcc agctgcggcg gatgcgggat
      121 gcgttccaga ccaccgcgct ggtgcacgag gcctacctga agctggccgg cggcgcgccg
      181 ctggcggcgc tggatcgcaa ccacctgctg gcgctgtcgg cgcgggcgat gcgccaggtg
      241 ctggtggacg atgcgcgcgc acgcaaggcg gacaagcgcg ggggcgggca ggaggcgctg
      301 accctgaccg catcgcttgg caatggcgat gccgccgcgg tcgaagtgct ggcgctggac
      361 gaactgctgg cctcgctgca ccgggtcgac gaacgcgccg cgcagatcgt cgagttgcgc
      421 tatttcggcg gctattcgga aaccgagatc gcggcgatgc tgggcatcag cgaccgcacc
      481 ctgcgccgcg actggcgccg cgcacgcgcg ttcctgcttg cggagctggg cacgtgagcg
      541 acgccgcgac cgaacgccgg cggcgcgcgc tggcgatctt cgacatcgtc gccgacctcg
      601 ccggcgaggc gcgcctgcag caaatcgatg ccctgtgcga cggcgaccag gcattgcgcg
      661 agcaggtgca ggcgctgctc gacgccgacg ccggcacccg cgagccgttc agcggcgacg
      721 tcgcggcctg gggccaggca ctgcaggcgc aaccggcgga tggcgcatcc gacccgatgc
      781 tcggacgcag catcggtgcc tggcgcgtgc gcgaggtgat cggccgcggt ggcatgggcg
      841 cggtatacgc ggtcgagcgc agcgacggtg cctacaccca gcaggcggca ctgaaactga
      901 tccgaaccgg cgccgattcg cccgccgcgc gcgaacgctt cctgcgcgaa cggcagatcc
      961 tggccggcct gcagcacccc aacatcgcca ccctgctcga tggcggcatc agcaccgatg
     1021 gcgaaccgta cttcgtgatg gaacgcgtcg atggcgaacc catcgatcgc tggtgcgatg
     1081 cgcgcgggct cggcctgcgc gagcgcgtgg tgctgttcct gcaggtgctg gacgcggtgc
     1141 gtttcgccca tcgcaacctg gtggtccatc gcgacctcaa gccctccaac ctgctggtcg
     1201 atggcgacgg ccgggtcaag ctgctcgatt tcggcatcgc caagcagctc gaaggcgccg
     1261 acgccaccgc cacccacgac cgcgcgctga ccttcgaata cgcctcgccg gaacagttgc
     1321 acgacgcgcc gatcaccacc gccaccgacc tctggcagct gggcgtggtg ctgcaccggc
     1381 tgctggccgg cgcgcatccg ttcgggctga cccgcgacac gccggtggca cggcaactgc
     1441 agcaactgga gcgcgagccg gaaccgctga cccgcgctgc cgcccaggcc agcaccgagc
     1501 aggccgcgca gcgcggcggg cacaccccgg cgacgctggc gcgcgcattg cgcggcaacc
     1561 tggcggcgat cgtgcaggcc tgcctgcggc gcgacccgga cacccgttat gcctccgccg
     1621 atgcgctggc caacgacctg cgcgcctggc tggacgaccg cccgatcgcc gcggtccggc
     1681 tctcgcgcgg ggagcgttcg cggctgtggc tgcgccgcaa ccgtggcctg gcggcggcgg
     1741 tcgccgcggt cgcgcttgcc ctgctggccg gtaccgggct tgcgatgtgg caggcacgcg
     1801 aggcgcgcga acaggcgcgc atcgccgagc gcgaaagcga gagcgcacgc gcggcgctgg
     1861 cgttcctgac cgataccctg gccgcggccg cgccggaacg cgcgatgagc accgaagtca
     1921 gcgttcgcca gctgctggac aaggcgcgcc agcagctcga ccagcgcacg ctggcgccgc
     1981 aggtgcggca gtcgatccag cgcatgctcg ggcacctgta cggatcgctg ggcgaggcgg
     2041 aaacctccgc acgcctgttc gctgcggggt tgcagggcgt gcagccacgc aaccgcgatg
     2101 acgccctcgc cctggccagg gacctggacg gccagtccgg cgtgctggcg gtgctggaac
     2161 gcggcaaggc cagcctggcc agcgcccgac tgggggtgga cctgcgccag cgcttcgccc
     2221 cggacgatcc gcgccagcga ttccttggcc agcggcaact cgcggtcgcc tacaccagcc
     2281 ttgccgactg caagcagggc gatgcgcaat ggaagcaggc gctggcgacc gcgacttcga
     2341 tgcgtgaccc gacggtcgac gagatcggcg aggcgcacgt tgcctacgcg cggatgctgt
     2401 tcaactgcgg cgaatacgcg cgcggcttgc aggtgtccgc ggacggcctg ggcttcgtcg
     2461 acggccggcg cctgccggcg ctgtcgccgc tgcgcgccaa cctgctgtgg gtcaaggcga
     2521 tgaacggcca tgcgatcggc aagctgtcgg tggcggacac cgaagccctg ttgcgccagg
     2581 ccatcgccat ccacgaaaag accatcggcg cgggcggcgc gcaactgggc gagttccatt
     2641 ccaccctggg cacggtgctg cagacttccg ggcgcctgcc cgaggcgctg gtggcgcacg
     2701 aacgttcggc tgaactctcg cgcgcggccg gcgcgcggcc gatcgaccag gcgatcaccc
     2761 agtacaacat cggctacctc taccgcgact acggcgatta cgaaaaatcg ctggccgcgc
     2821 accggcgcag cctggccatc ttcgatgcgg ccggcatcga ccgcgaccac atcgagcgcc
     2881 ggcgcgcgga gaagtggtac gcgctggcgc agatccagtc cgggcgcgcg gcggacgcgc
     2941 gcgagcagct gctgcgcctg cagcaacgcg cgcgcgcgct cgacggcgag gaatcggtcg
     3001 aatacctgga cgtggtctgg caactgctgc gggcggcggt ggtgatgggg gatcgcgcga
     3061 acggcgaacc gctgctggcg gatgcacgcg cgcgcgcggc caggttcgtg ccgcccgccc
     3121 atccggtatt caccgaattc ctgcgcgacg aggcgcagtt cgcacgcctg cagggcgacc
     3181 tggccggtgc cgaacgcctg cagcgcgagg cactgaagcg cagcgacgcc atcggcaacc
     3241 ccaccgagcc gtccatcgtc cgcgtggagc tcgcggacct gctcgtccag cgcggccagg
     3301 acgcggaggc gcgcatcctg ctgctgcagg cgctgccggt gctgcgcaag tacatgcatg
     3361 cccggcacgc cacgcgcgcg cgatccgagg tgctggcccg caggcttggc gtctagccgc
     3421 gcgtaagcgg ccggatcggc gcacgcgtca gtcgcaggcc tggtccaccc gcgtcaccga
     3481 gaacacgggc tccaccgaat ccgagaaccg ccccatcggc gttgcgatgc tggagcttcc
     3541 acgcgcctcg atccggccgc tgccatcccc atccagtgcc agcgtgtagc tgccaccacc
     3601 cgaccaggcg accccggcgg cattgccgga ctgcgtccag gaacccccgc cctcgccacc
     3661 ggacatgtgc atcacgatgc cggccgtgct cctgatgtcg aacggcccgt ccagcgaaca
     3721 gaccaccgta ttggcgacga aatcgttctg cccgcccttg acccggtagg cgccttcgtt
     3781 ggtgtcgaat cccaggctgg ccttgccgac cccgcgcttg ctgcgcgact cgaaatcgat
     3841 gcttgccttt tcccttttct tcgccggccc ggcgtactgg tacttcgcat cggcgcgcac
     3901 cttgccactc gcaggctgca ggctggcacc gccgctcagg gtggccgcca ccgtgccgcc
     3961 cgccgccgcg ccatcggctt tcgcacgcgg cctggcgtcg atgtcgaacg ccgtgttcgg
     4021 cttgatcccc ttgcgcttgc cgggactgct ggtgacctgc aggtcgatgc agcggccgct
     4081 ctcccacggc gagccggcgg acgcccccag gccacgcagc atgacttcgg caatcagggt
     4141 ctgcagcgtt tcggcaccct gcagcagcgc ccgggtgcgt tcgacttccc ccggccggaa
     4201 gatgctgaag ccctgttcgc cgtggtgttc gaagatcggc ttgccgccgc gctcgcggcc
     4261 ggtggtgata tccaccgact ggctctggaa attctcggag ccgcccatgc tgatgcgcag
     4321 gttcgacgcg ccaccttctg cggtgtcgat caggtgggcg tcgtcgtcga ggtagcgctc
     4381 gtacttgaaa ttcgaacgca cgtagccgcc ggtgcccggc ttgccgctga cgctcatctg
     4441 cgagtcgaca tcgatgtcca ccgacacccg gccctgcgga tcggggcagg catccatccg
     4501 cgtcttgatc ctgaccttgc cgttcaggcc gttttcgttg acctcgaatt ccatcgactg
     4561 gctgagcgaa ccatccttgc cgaattccac cgccgaattc ccgtcgttgc ggccgaactt
     4621 gatcggcccc tgcttgacca gctcggccaa gtccttgtcc gacatgctgc tgaccgcgcc
     4681 gctgagcatg ccgccggtca tcaggccgac gaagccaccc aagcccatgc cggtgaagcc
     4741 gctgtccatg ccttcgccgg tgaacgccgc cgggaccagt ttcggcaggt ccgcctcggc
     4801 gccgcgcaac ttgcgctcgt aggcatcgcc cagtgcctgc agggccgcga tcgcctgcgc
     4861 ttcgccgccg agttcgcgtt cgacgccggt tgcctgcatc agcttgtagt gcagccagta
     4921 atccaccggc ctgagcttct gcaaggtcgg ttccgggcct tccgcaagca cacggtccat
     4981 gtccgccttg cccggcaggt gcgatggcac cgccgctgcc ggcgcgacga ccgcctccgc
     5041 ttgcggcgat ggcgacggcg atggcgaaca ggcgcaaacg acgaccagca acgctcctgc
     5101 cagggccgcg gcaggggcag gcaatggatt gcgcatggca tcgctactcg agggtcgtct
     5161 ctccagttag cgcaaagcgg ccgcgcaaca ggacacccgc ccgcaagccc gcactcaacg
     5221 cgcgaccagc gcccccttca gggccgccac gccgtcgatg ctgtcggtcc atgccagcca
     5281 ggcgccgctg gcgttggcga ccagcttcgg ggtgccgctg gcgtggccgc gggcgtccag
     5341 tcttgccacc accatgcgtt gcagttcgcg cgagaggtcg ggtgcatagc gcgccagcat
     5401 cagggtctgc ccgctcgcgt cctcgcgcag ccaggtgacc caggcctgtt gcgcatcgag
     5461 cgcgacatcg acccgtccga gcaccgccgc gcccttgtcg acgatgaccg gggcagcgaa
     5521 cgcatcgccc gcatccgtgc tgcgcgcgat ccgcaccgcg gcgatgccgc cgccttcgct
     5581 gtaccagccg accaccgcgg tgttgccgcg tgcggcgacc gcggggccgt tgaccgggca
     5641 ggcctcggtc ttccagccat cggcgtggac cgccttcggc gcgctccagg caccggcctc
     5701 gaagcgcagc acggcgatgt cgcggacctc gtcgtcggcg cgatcgcggt atgcgagcaa
     5761 cggtcccttg ccggtcagcg ccacgtcggt ctggcaacag tcgcaggtgc gcgcatccgc
     5821 caccatgtcg gtgccgcggc ccaggtccat gtcgaaggcg ttggcgcgca gctgcatcgc
     5881 gccggtgcca tcgccctcgc cggcatgcgc gccttcggca tgcgccgccg cctgcgcgcg
     5941 gccatccagc caggcgatgc cgatgcggtc gctgccggcc gcccacagcg tcgcgaagcc
     6001 atgctccacg ccaaggccat cgtcattgac gttggtgatc tggttccagc tcatgccgcc
     6061 atcgcgcgag cgcgccagca cggtgtcgta accgctgggg ctgtcgggac gcgcctgcag
     6121 ccactgcacc cagatcgcgc cgtcgggggt gcccagcaag tgcggtgtat ccgccccgtt
     6181 ggcgaccagc gactgcccga ccgcgatcgt gcgcggctgg ctttgccaac cgccggcatc
     6241 ggtgtaggtg gcgaactgca gcgcggtgcg gcggccttgc tggcggttga gccagctcag
     6301 cagcagccgg tcgccgctcg ccaggctcag gtccggcgcc atcgaaccgg cggcggcggg
     6361 caatgcccag acctgcaccc ggtaatcgcc gttcgggttc gctgccgggg tgtcggcatc
     6421 gcgcttgcag gcgggaacca gggcgatggc gacgaagatg gcggctgcga tgcgcgccct
     6481 ggtgtgcatg cttttcatgc gcaaaggata cgcccgcgct ccgcttcgat tccgttgcta
     6541 ggccgcgcgc tcgcggcgct ggcgttccag gtgcaccagc aacagcgaaa tcgccgccgg
     6601 cgtcatcccc gggatgcgct gcgcctggcc cacggtctgc gggcgcaccc gctggagttt
     6661 ctgcagcacc tcggcggaca gcccgcgcac gccggcgtaa tcgaacgcgt ccgggatcgc
     6721 ggtggcttcg tggcggcgct ggcgcgcgat ctcctcgcgc tggcggtcca ggtagccggc
     6781 gtacttggtt tcgatctcga cctgttcggc aaccttggcg tcctccaccc ccggcccaag
     6841 ctccggcacc tgcatcaagg tcgcgtaatc cagttccggc cggcgcagca ggtcgatcgc
     6901 gttgccctcg cggctgacct cgatgccgat gctgcgcgcc agcgccgcac ccagcgcgtt
     6961 gttgggtgtc gcccacagcg cggacagccg cgcggtttcg gcaatgaccg cctcgcgctt
     7021 gcgctcgaac ctttcccagc gcgcgtcatc gaccaggccc agttcgcggc cggtcggcgt
     7081 cagccgcgca tcggcgttgt cctcgcgcag ctgcaagcgg tattcggcgc ggctggtgaa
     7141 catgcggtac ggctcggacg tgccgtgggt gatcaggtcg tcgaccagca cgcccagata
     7201 cgcctcgtcg cgacgcaggc tccacgcctc tttgccatgt acgaagcgcg cggcgttggt
     7261 gccggccagc aaaccctgcg cggcggcctc ctcgtagccg gtggtgccgt tgatctggcc
     7321 ggcgaagaac agccccgcca ccgccttggt ctccagcgac gccttcagcc cgcgcggatc
     7381 gaagaagtcg tattcgatcg cgtagccggg acgggtgatg tgcgcctgtt cgaagccgcg
     7441 gatgctgcgc accagcgcca gctgcacgtc gaacggcagc gaggtgctga tcccgttcgg
     7501 atagatctcg ctgacggtca acccttccgg ttcgacgaag atctggtgcg acgccttttc
     7561 ggcgaagcgc accaccttgt cctcgatcga cgggcagtag cgcgggccga tgccctcgat
     7621 ctggccggaa tacagcgggc tgcgatgcag cgcgtcgcgg atgatctggt gggtgtgctc
     7681 gctggtgtcg gtgatccagc agctgacctg cgccggctgg tcatcatggc tgcccatgaa
     7741 cgacatcacc gggcgcgggt cgtcgcccgg ctgctcggtc atcaccgcgt agtccagcgt
     7801 gcggccgtcg atgcgcggcg gcgtgccggt cttgagccgg tccaccacga agcggccttc
     7861 gcgcagcttg tgcgccagcg cggtggaggg cggatcgccc atgcgcccgg cggcgtactg
     7921 ggtttcgccg atgtggatct tgccggcgag gaaggtgccc gcggtcagca ccaccgccgg
     7981 ggcatggaag cgcaggccgg tgttggtgat cgcgccgcgc accgcgtcct gttcgatgac
     8041 gaggtcatcg accgcggcct ggaacacggt gaggttcggc tgggtttcca ccgcgcggcg
     8101 gatgaagctg cggtacagcg aacgatccgc ctggcagcgg gtggcgcgca ccgccgggcc
     8161 tttcgatgca ttgagcgtgc gccactggat gccggcggca tcggcggcct tggccatgat
     8221 cccgccgagg gcgtcgatct ccttcaccag atggcccttg ccgatcccgc cgatcgccgg
     8281 gttgcagctc atcgcgccga cggtgtcgat cgaatgcgtg agcagcagcg tgcgcgcacc
     8341 ggcgcgcgcg gatgcaagcg cggcctcggt gccggcgtgg ccgccgccga tgacgatcac
     8401 gtcgaagctg tagaaggagt cattcatggc ggccgcattc tagtccggtt gccgatcggt
     8461 cgattcggtg cgccttgcgc acggttggca cggttcctgc gtaatcccct gcagcactcg
     8521 gcgtggcagc gcgcaagcgc cttgaccgga ctgaacaggg gccggtacag cgcacgtcgg
     8581 ggatgctcgg gggccggatg tcgggggaca ttcggccccc tctttttttg cggattcgac
     8641 agcgcgcgca gggcgcgatc gctggaagga agtgccggcg actgcggggt tgacggaaca
     8701 ggggggatcc ggaggtcccc acaggtcgcc ggcagcacga agtttagcgc cttcgccaac
     8761 cggtgtgacg cgtttggtca gaagcgcgcc gacaggcggt cactgtgtga tcgcggtcac
     8821 gttcattcct cgggcacggg cgacgccttc gcattgcgga tcgcgcgacc catgcgcgaa
     8881 ccaccaccat cgtcgctgcg catgcgtggc ggcggtggcg cggtcgtgcg ttgctcgcgt
     8941 tgctcgcgtt gctgcgcggg cagcatcgat ggtgtcgatc ccgcctgcat gcggcgcgcg
     9001 cgcggccggg tgtcgtcgcg atcctccatc gcttgtccac cgggtgccga caggccgccg
     9061 gggttgcgcc acggcggcct gcgatcgctg ccatcgccat cttcgcctcc gtggccatcg
     9121 ccgtcatggc catcgccgcc atggtcgtgg ccgtcatgcg gcgcgcgcgg gcgataccac
     9181 cacgggctgc cgtatcccgg cggatagccg aagtagccgt agtagctgcc gtaaatcacg
     9241 cgcccgtaac ggtcgtaatg gaccccgggc gcatagccgc cgtaaccgaa accgccgtag
     9301 aagccgtacg ggtcgtggta gcgatactcg gtctgcggct ggccgtagta gtagtcgcca
     9361 ttgccgccgc ggtaggtgta cgccgtcgcg cagccggcga gggtggcggt ggcgagcatt
     9421 gacagcagga tcctgcggaa catggcgtgc ttccccttcg gtaccccggc agcttctgcc
     9481 cgcggctttg aatccgtgct taatccggcg ccagcagggc cgccgctgcc ttgccgcgca
     9541 gcggctgcgc tagcatgcgg cgatgacacc gacagccccg atgccgaact tcaccgacgt
     9601 gctggccgcc gccgcgcgga tcgccgggcg ggcgcacgcc accccggtga tcacatcgcg
     9661 cgcgctggat gcgcacgccg gtgcgggctt gctgttcaag tgcgagcagc tgcagcgcat
     9721 cggcgccttc aagttccgcg gtgcctgcaa cgcggtgatg tcgctggacg acacgaccgc
     9781 cacgcgcggc gtggtcaccc attcctccgg caaccacggt gcggcactgg cgctggccgc
     9841 gcaggaccgc gggattccct gccacgtggt ggtgccggac ggcgcggtgc gcgccaagct
     9901 tgccgcgatc gaacactacg gcgcgatcct gcaccgctgc gcagcgacga tcgcggcgcg
     9961 cgaggcgatg tgcgcgaagg tgcaggccga caccggcgcg accacggtgc atccctacac
    10021 cgatccgcgg gtgatcgccg gccagggcac cgccgcgctg gaactgctga ccgcgcatcc
    10081 cgacctcgag gcgctggtgg tgccggtcgg tggcggtggc ctggccgccg gcaccgcgct
    10141 ggcggtgcgg gcgctggcac cggactgccg gctgtacctg gccgaaccca gcggcgccgc
    10201 cgacaccgcg cgttcgttcg cacgcggcac gctcgaccac gacatggtcc cggacaccat
    10261 ctgcgacggc ctgcgcggca ccttgggcgc gatcaatttc gaaatcctgc gcaacgccgg
    10321 cgcgcaggtg ctgaccgtcg acgacaccca ggccgtggcg gcgatgcggc tgctgtggac
    10381 gcgcaccaag cagttgctgg aaccgtcgtc cgcgaccgtg ctggcggcag tgcttgcgaa
    10441 tcccgcattg ttcgcgcggc ggcgggtcgg catcatcctg tccggcggca acgtcgacct
    10501 cgacgccctg cctgccctgt tcgcacttgc cggggacgcg cagccatgaa gatcgacgga
    10561 acccgcgtgc acgatcgcgc caccgagctg gtgctcgcct actacgcggc gttcaaccgc
    10621 ggcgactggg ccgcgatgca ggcgctgctc gccgacgacg tggtccacga cctcaaccag
    10681 ggcccgcgcg aggccggcaa gcccgcgttc gccgcgttcc agcaacggat ggcgcgctgc
    10741 taccgcgaac agttgcgcga cgtggtggtg ctggcctcgc cggacggcct gcgcgccgcc
    10801 gccgaatacg tggtgcatgg cgaatacctg caggccgacg aaggcctgcc gcccgcgcac
    10861 gggcagcagt acgcattgcc cggtggcgcg ttcttcgagg tgcgcgatgg cctgatcgcg
    10921 cgcgtcagca actactacaa cctgcaagac tggctcgccc aggtgcgctg agcgccgcgg
    10981 cgggtcagcg ccgccggatc gggatccgcg acaacgggat cgaggcgacc tcgacatcgc
    11041 cctcgcgcag cggggtgccg gcctccggcg cccacgccat cgcggtgatg gtttcccagg
    11101 tcgccggcag gccggcgcca tcgcgatggg tttcgtaggc atccgccgcg accgtgaagc
    11161 gcgcgcgccc ggtcagcgtg tgccggcgcg aggccagcgc attggtcgcg ccgatcgcgc
    11221 gcagctcgcg catcagcgag gccaggtccg ggtaatgggt ggtcagttcc tcgcggtcca
    11281 gcaccggctg gtggaacccg gcgcgcacca gcgcatcgcc gaagccggcg atgtccgcga
    11341 acggactgac gtgcggcgcg gcgtcggcgc gcgcgaacgc ctcgcgcagc tcccacaggg
    11401 tttccggacc gaaggtggag accagcagca gcccgtgcgg cttcagcacg cggcggaacc
    11461 cggcgaacag cgcgtccagg tcctccaccc actgcaggca caggttcgag aacagcacgt
    11521 cgaccgaagc ctcggccaac ggcagcgcgc gcgcgtccgc gcataccggc accggcgtgc
    11581 gcgcgaacgg attggacagc cacgcgccgg ccgggcgccc ggatgccacg cgcgcctgct
    11641 gcagcatcgg cagcgcgagg tcgagcgaca ccacctgcgc cttcggccag cgcgcctgca
    11701 tcgcgaggct ggcgcggccg gtgccgcagc cgaggtcgag cacgcgccgt ggcgcctcgc
    11761 gcttgagcgc gggatcgtcc aggtagtcca gcgattccag cagccgcgcc tccaccgcat
    11821 gttgcaggcg tgccgcggcg tcatagccgg cggcggaacg cgagaacgcc cggcgcacct
    11881 gtttcgcatc gaacaggcgt gcgctcatgg ctgcgctgct cccgcttctg catgggtggc
    11941 ggggttgggg cggggatcgc aggaggcgag gaagtccagc aacgcgtccg ccaccgcatc
    12001 cgcatgggtc aggaacggcg cgtggccggc gtgctccacc tgcaggaagc gcgcgtccgg
    12061 cgcgagcgcg gccgccgcct gcatcgccag cggattcacc agccggtcgc ggcggccgcc
    12121 gatccacagg ctgggcatgc gcaacgcggg caatgccgcg cgcaggtcgc tgccttccag
    12181 caaccgcagg ccttccgcca atacccgcgg cgagggttcg ccatgtgcca gcaccgcatc
    12241 acgcagcatc cgcagctcct cgcgcatgtg gtcggaaccg aaggcttcca gcgcgatgaa
    12301 gcgatcgaca ctggcgcgcc agtccgcttg caggccatcg gcgaaaccgt ggaagacctc
    12361 cgccggcatg ccctgcggcc agtcgccggc gaccacgaac ttgggggtgg cgcagaccat
    12421 cgccagcgcg tggatgcgcg cgggctgttt cgacgccgca tgcagggcga ccagcccgcc
    12481 cagcgaccag ccgcaccagg gcgcgtcggg cacggcatcg agcaccgcat cggcgcaggc
    12541 atcgagcgcc agcggcagcc cgctcgcgcg gctgcggcca tggcccggca ggtcgaccac
    12601 gtgcagcgtg cgccgcgcgc gcagcgcctg caccagcggc gcgaagatgc caccgtgcat
    12661 cgcccagccg tgcagcagca ccagtggtgg cccgctgccg gcgacgtcca cgtgcaggct
    12721 catgccgcca ccgcgtcgcg cgcgcgcgcc agtgcatcca gcaggccatc gacctgcgcc
    12781 ggaaggtggc cggccgcgag ggtgatgcgc agccgcgcgc tgccttccgg caccgtcggc
    12841 gggcggatcg gcgcgaccca atagccttgt tgttcgagtg cggccgccag cgccagcgca
    12901 tccgcatcgc cgcccaccag caacggctgg atcggggtgt cggacggcag cagcgcaagc
    12961 ccgcgtgcgc gtgcgcccgc ctgcagccgc ccgatgttgg cggcgaggcg cgcgcgcagg
    13021 tcgtcgcccg cgcgcgcgat gcgcacggct tcgcgcgtgg ctgccgcctg cgccggtggc
    13081 agcgcggtgg tgtagatatg gggccgcgcg gtttccgcca ggtgcgcgac caggtcggca
    13141 tcgcccacca ctgccgcgcc ggcgctgccc agcgccttgc cgaaggtgac cagcctcagt
    13201 ggcacgtccg ctgccgccag ccccgcggca tcgatgctgc cgcgtcccgc atcgccgagc
    13261 acaccgacgc catgtgcatc gtcgatgtac agcgtggccc gctgcgcgcg cgccacctgc
    13321 gcgagtgcgc gcagcggcgc gacatcgcca tccatcgaga acacgccgtc gctggcgagc
    13381 aacgcggcgc cctccgcctg cgacgccagt tgccgcgcgg cgccttcggc atcgccgtgc
    13441 ggatagcgct tgaacgcgca gccggccagg cgcgcgccat cgatcaggct ggcgtggttg
    13501 cgcttgtcct gcacgcagag gtcgcccgcg cccagcagcg cctgcaccac cgccaggttg
    13561 gccaggtagc cgctgccgaa cagcagcgcg cgcggcgcct gcaaccagtc ggcgagttcg
    13621 gcttccagcg cggcgtgctg cgcgccatgc ccgcacacca ggtgcgaagc gccggcgccg
    13681 gaaccttcgc cggcggcatc gcgcagggcc gcgatgaccc gagggtgttg ggagaggccg
    13741 aggtaatcgt tgctgcagaa attcagcagg tcacggccat cgacggtgca cacgacgccc
    13801 gcgcgccggg tcaccgtgcg cagatggcga cggcgatgcg cggcctcgcg c
//
LOCUS       DAORUK010000052        13272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_4598_length_13272_cov_19.109329, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000052 DAORUK010000000
VERSION     DAORUK010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 13272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13272
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4598_length_13272_cov_19.109329"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /gene="dnaX"
                     /locus_tag="PK818_05140"
     CDS             <1..1496
                     /gene="dnaX"
                     /locus_tag="PK818_05140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016944813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HQY82153.1"
                     /translation="LAIDAGRYIDLLEIDAASNTGVDNVRELIENAQYMPSRGKYKVY
                     LIDEVHMLSKPAFNALLKTLEEPPGHVKFLFATTDPEKLLVTVLSRCLQFNLKRLDEA
                     QIRGQIVKILGAEAIEADDAAVRQLAHAADGSLRDGLSLLDQAIAYTGGRLDADAVAA
                     MLGTVDRNRVNELLCALADGDGARLLAEAAQLAEFSPDWGSVLDALAEALHRIQVKQL
                     VPAVDIAGDGVDVEALAARLRPELVQLWYQMALHGRRDLGYAPSPRSGFEMSLLRMLA
                     FRPGEATVQASLPRAVATAGAAVTAAGAPAAPRGHAATAAAEQARAAVAAAVAPQKPA
                     PPPQSAPPPMQWAEPSAPSPPPAQAVAAASAMLVTDSEHWLELVARSSLRGPARLLAE
                     HAAFVSHADGVLRLSLAAGDEHLQSPSLLLQLGDGLASMLGGPVQLRFETGDARGDTA
                     RVRNARDREQKQAGAEQGFAADPDIQRLVQLHGAQIVPDSVRPPGEG"
     gene            1552..1872
                     /locus_tag="PK818_05145"
     CDS             1552..1872
                     /locus_tag="PK818_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011346622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HQY82154.1"
                     /translation="MRGNIAQLMQQAQKMQEDMQRAQAEVAALEVVGNAGGGMVSVTL
                     GGRMDCRKVRIDPSVLADPEMAEDLVAAAFNDAVNRINAESQAKMSAATAGMSLPPGM
                     KLPF"
     gene            1958..2569
                     /gene="recR"
                     /locus_tag="PK818_05150"
     CDS             1958..2569
                     /gene="recR"
                     /locus_tag="PK818_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HQY82155.1"
                     /translation="MTASLLEQLIDALRVLPGVGQKSAQRMAYHVLERQREGGRRLAE
                     ALAQAVERIGHCRRCRDFSETDLCPTCSSGARDAQLLCVVESPADRLAIEQATGFRGL
                     YFVLQGRLSPLDGIGPHELGLDQLAQRLAEGEVQELIVATNPTVEGEATAHYLAQLAR
                     AHAVHPSRLAHGVPLGGELEYVDRGTLSHAFGSRSEIPKEPPR"
     gene            2566..2916
                     /locus_tag="PK818_05155"
     CDS             2566..2916
                     /locus_tag="PK818_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HQY82156.1"
                     /translation="MSDTLFHKIIRREIPADIVYEDEHLIAFRDIAPQAPVHVLFVPK
                     ADFATLDDVPEEQALVVGRLATAAAAYARRQGFAGDGYRVVMNCNRDAGQTVFQIHLH
                     LLAGAPLGRFGTAG"
     gene            complement(2920..3408)
                     /locus_tag="PK818_05160"
     CDS             complement(2920..3408)
                     /locus_tag="PK818_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HQY82157.1"
                     /translation="MNIRIPVAGIAVVLLAACATQPKPLQGTFQPLTAHEASAGDHTG
                     ALVRWGGRIIRTEPRPDQTCFEVISTRLSADGRPHWASDDTWGRFIACRTGFYDPALF
                     QPNREVTFTGRIEGHEERKVEQYVYRFPRLAADVVYLWPERERVDVVVRPAPWPWAWG
                     YW"
     gene            complement(3476..5452)
                     /locus_tag="PK818_05165"
     CDS             complement(3476..5452)
                     /locus_tag="PK818_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HQY82158.1"
                     /translation="MPEFRPLLARLKSPPMQVRGRALVLLATAACLLPLLLQLPRELG
                     IGFGVAAVLVAAVSWRRVPPMLLRLLVGIGMIVAIAAVAPGVGRDTACAVLAAMLALK
                     PSETVSLRDGRSLVGFALFAPFAAFLLDQGPASLLLALLAMLAALLALQQLAHDEAEV
                     RMPGAAQAWQATAGVFKLVLLGLPLALAAFWLFPRLPTPLWGLPERVVARPGLSDSMT
                     PGGWLDLMNDDSPAARVQFFGPAPPQHQMYWRGPVLWDFDGRTWHQLKQWRFQQAIPK
                     RLKGPAWDYVIEPEPTEGRQLITLDLPDQVPKDAFISHDYSVYSVMPLNNVSRWRMRS
                     LAATTVDPQLPPILRMHALQLPRGFNPRTIALGRQWRREAGTDATGNADAAIANRALA
                     MIRREFAYTLNVPLAGRNEIDDFLFDRKQGYCEHFSSAFVVLMRAAGIPSRVVTGYAG
                     GYRNPIGDYWLVLNSDAHAWAEVWLPRRGWVRVDPTAAVAPERVFDTLADRQPGRIGG
                     FDGLVPMFNASDWMRRGWNDFVLGFNAQRQQRLLSPLGLERIGSRGLILLFAVIAALA
                     LGWMGWLLARGEREQDPLLRAWHRLGRRYARRGQARGMHEPAEAWAERVAADVPRAGE
                     HLRALSGRFSAARYAGIGPAELRRLLRDLAAHRP"
     gene            complement(5445..6401)
                     /locus_tag="PK818_05170"
     CDS             complement(5445..6401)
                     /locus_tag="PK818_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQY82159.1"
                     /translation="MIAWLRRRLQSWMRPRQAESLPVRLRRNRIYVLPTATGLFFALL
                     LGTMGLGALNFNNNPGLLLALLLAGAAQASLIAAHMQLSGVVVDAVAADPVPAGEPLR
                     LRISLGARDQRMRRGLRISHGDAHVHAAFDHHGGTVELPIPTGRRGLMPLPRLELRTL
                     QPLGLARAWAYVWPRQVALVYPAPEMQAPPLPAPAGDAGRAQVTRAGDDVHHLRGYRA
                     GDAPRSVAWKASARRDSLLVREYEQHRSDQLLLDWPLTAGLPYEQRIRRLARWVDDAE
                     RQGRRYALGLPGQAPVPMGLGMAHRHRCLRALALMPGEAAHA"
     gene            complement(6405..7355)
                     /locus_tag="PK818_05175"
     CDS             complement(6405..7355)
                     /locus_tag="PK818_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY82160.1"
                     /translation="MSAPPATDTMLPEALRVALRDAQAQVNTLVLGKPQQVRLAFVAL
                     LADGHLLIEDLPGLGKTTLAHAMAATLGLGFQRVQFTSDLLPADILGVSVYEAQARRF
                     EFHPGPVFTHVLLADEINRAPPRTQSALLEAMAEHQVSIDGATRALPDPFLVIATQNP
                     VDLAGTYPLPDSQLDRFLLRLALGYPDEQAERALLAGSDRRDLIAQATPLLDAERVIA
                     LRRAVLAVHASEALVGYVQALVGRSRKHPGVRVGLSPRAGLALLRAARAHALLLGRGH
                     VVPEDVQALFAAVAEHRLVGEADTSAAALAKAIVHAVPVD"
     gene            7431..9260
                     /locus_tag="PK818_05180"
     CDS             7431..9260
                     /locus_tag="PK818_05180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQY82161.1"
                     /translation="MQETDFAKRWFVGPWLALLGLKLWLASRLPLFVDEAFYWQEGRH
                     LAWAYSDLPGLTAWLARIGDGLGDGTLALRLPFLAIAAAIPWLVVRLAARELDPKAGW
                     QAGTLALLLPLAGTMGLLAVPDVPLLLATLLCLDAGARLLRRVDAFAALELALGLVIG
                     GLTHYRFAAVIAVGFCALLVLRDGRAALRDRRVWIALAAGALAWLPLVLWNLDNADAG
                     LRFQLVDRHPWSFSGAGVRLGRMQLLLATPLLLLAMVVAAWRGWRDGRPAARYLAASG
                     SMIVLGFLLLGFFADRERVSFHWPLPGYLALLPLVPAVLAGWPRGWRIATWATAAVGL
                     GAVLGYYAVASVPSWRAQTASRNFHPTNFAGWRELDAAVRDALATMPADTRLLAGNFK
                     IGAELGFARDDAGIAVLEHPLNDKHGRAPQLALWGISHASRATLGDGPLLLVASSSDA
                     RFRELLAHYHALCRQVGPLPPPRVLNIDHGRQRFLLFAIPRGIRSGACTAPALAFLDA
                     PVAGKAVAGGFEVSGWAFKDGVGLRGVEVMLDGRVVAQARYGETNPGVTGYWKDSNDR
                     NHPNVYFRARVEGAQPGARWLGLRLHGADGSVEDWPEQKIEVR"
     gene            complement(9270..9848)
                     /locus_tag="PK818_05185"
     CDS             complement(9270..9848)
                     /locus_tag="PK818_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009608202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HQY82162.1"
                     /translation="MARLVLASSSRYRRELLQRLRLAFDVASPAVDETALPGEAPGML
                     AARLAQAKARAVALQLGGDTWALGSDQVAEVEGRALGKPGGRDAAIAQLDSMSGRVVR
                     FHTALCLAHADGRLHAGCDLTEVHFRVLEAAEIERYVDAEQPFDCAGSFKSEGLGIAL
                     FERIDNRDPTALIGLPLIATCRLLRAAGFALP"
     gene            complement(9851..10213)
                     /locus_tag="PK818_05190"
     CDS             complement(9851..10213)
                     /locus_tag="PK818_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82163.1"
                     /translation="MNLVLPALLAATLAVVAWLSWRARQRGQGRQRAIRGVLDAADAL
                     EARLRVAREELEAVAGDHENPVRGAMQEILRQRLWLQDNAGSADLAQLDAVRRTLESA
                     RTSLEQQLQRVSQARAGH"
     gene            10333..10851
                     /locus_tag="PK818_05195"
     CDS             10333..10851
                     /locus_tag="PK818_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011346632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HQY82164.1"
                     /translation="MSDPSISRVPESVDAWRMVAAQREFEGRIPLASMARLRDGLLEP
                     EGDARFTLAFGTDALRLPYVELRVEAELPLECQSSLQRFLLPVQLVQRLGLVRDEADE
                     AALPEGYEALLVDADGELKPAELVEDELILALPVVAVAPGAEVVEREFVPGAEETAEA
                     NPFAALAGLKKR"
     gene            10915..11109
                     /gene="rpmF"
                     /locus_tag="PK818_05200"
     CDS             10915..11109
                     /gene="rpmF"
                     /locus_tag="PK818_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009608206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HQY82165.1"
                     /translation="MAVQKSRVSPSRRGMRRAHDALSAKQLATDPTSGETHLRHHVTA
                     DGYYRGKQVIAPKTQVADEE"
     gene            11266..12255
                     /locus_tag="PK818_05205"
     CDS             11266..12255
                     /locus_tag="PK818_05205"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HQY82166.1"
                     /translation="MSQSQGRIFSRIAGTGSYLPEKVLTNADLTEFVETTDEWIVART
                     GIRERHVAAEGETTSDLAYHAAVRAMEAAGVTAADIDLIVLGTTTPDLIFPSSACLLQ
                     HKLGANGCPAFDVNAACSGFIYALTIADKFIQSGAAKTALVVGSETLTRMLDWSDRGT
                     CVLFGDGAGAVVLKADGETGILSTHMHADGGKKELLWNPVGVSVGFKPDEKNAGVRVL
                     MTGNEVFKHAVKALDSVVEEALLANGLDRHELDWLVPHQANLRIIEATAKRLEMPMER
                     VIVTVDRHGNTSSGSVPLALDEAVRAGKIQRGQLVLLEAFGGGFTWGSALLRY"
     gene            12551..>13272
                     /locus_tag="PK818_05210"
     CDS             12551..>13272
                     /locus_tag="PK818_05210"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HQY82167.1"
                     /translation="MLAELSELHPSVKATFEEASDGAGVDLWALSQAGPEEMLNRTEY
                     TQPALLAAGVAVWRLWQAQGGAQPAVLAGHSLGEYTALVAAGALSLRDGAHLVRIRGQ
                     LMQDAAPAGVGAMAAVLGAEDALVEAACVEASGAQVVVPANYNSPGQVVIGGDAAAVD
                     RALALLQEKGVRKAVKLAVSVPSHTPLMREAANRLANTMAGLAWRLPALPVVQNVDAR
                     AHDSVDAIRDALVRQLYLPVQWT"
BASE COUNT         1935 a         4610 c         4814 g         1913 t
ORIGIN      
        1 gcctggcgat cgacgccggc cgctacatcg acctgctgga gatcgacgcc gcgtccaaca
       61 ccggcgtgga caacgtccgc gaactgatcg agaacgcgca gtacatgccg tcgcgcggca
      121 agtacaaggt ctacctgatc gacgaagtgc acatgctgtc gaagccggcg ttcaacgcgc
      181 tgctgaaaac gctggaagag ccgccggggc acgtcaagtt cctgttcgcc accaccgacc
      241 cggagaagct gctggtgacg gtgctgagcc gttgcctgca gttcaacctc aagcggctgg
      301 acgaagcgca gatccgtggc cagatcgtga agatcctggg cgcggaagcg atcgaggccg
      361 atgacgcggc ggttcgccag ctcgcgcatg ccgccgacgg cagcctgcgc gacggcctgt
      421 cgctgctgga ccaggcgatc gcctacaccg gtggccggct ggatgccgac gcggtggcgg
      481 cgatgctggg aacggtggac cgcaaccggg tcaacgagct gctgtgcgca ctcgccgatg
      541 gcgatggggc gcgcctgctc gcggaggccg cgcaactggc cgaattctcc ccggactggg
      601 gcagcgtgct ggatgcgttg gcggaagccc tgcaccggat ccaggtgaag caactggtgc
      661 cggcggtgga catcgccggc gacggcgtcg atgtcgaagc gcttgccgcc cgcctgcggc
      721 ccgaactggt ccagctctgg taccagatgg cgctgcatgg ccgccgcgac ctgggctatg
      781 cgcccagccc gcgcagcggc ttcgagatga gcctgctgcg gatgctcgcc ttccgcccgg
      841 gcgaggccac ggtgcaggcc tcgctgccgc gtgcagtggc gacggcgggt gcagcggtga
      901 cggccgctgg cgcgccggct gcaccgcgcg ggcacgcagc gaccgctgcc gccgagcagg
      961 cgcgcgctgc ggtggccgcc gcggtggcgc cgcagaaacc agcgccgccg ccgcaatcgg
     1021 cgccaccacc gatgcagtgg gccgagccgt ccgcgccttc gccgccgcca gcgcaggcgg
     1081 tcgccgccgc cagcgcgatg ctggtcaccg acagcgagca ctggctggag ctggtcgcgc
     1141 gcagcagcct gcgcgggccg gcgcgcctgc tggccgaaca cgcggcattc gtttcccatg
     1201 ccgacggcgt gttgcggctg tcgctggcgg ccggcgacga gcacctgcag tcgccgagcc
     1261 tgctgctgca acttggcgat ggcctggcct cgatgctggg cggcccggtg cagttgcggt
     1321 tcgagaccgg cgacgcgcgt ggcgacaccg cgcgcgtgcg caacgcgcgc gaccgcgagc
     1381 aaaagcaggc cggcgccgag caggggttcg ccgccgaccc cgacatccag cgcctggtgc
     1441 agctgcacgg tgcgcagatc gtgccggatt cggtccggcc gcccggcgag ggttgattcg
     1501 ggcggccgtg gccgcactcc tatttccaat cgaacaagac ggaaccccga catgcgtgga
     1561 aacatcgccc agctgatgca gcaggcacag aagatgcagg aagacatgca gcgcgcgcag
     1621 gcggaagtcg ccgcgctgga ggtggttggc aatgccggcg gcggcatggt cagtgtcacc
     1681 ctcggcgggc gcatggactg ccgcaaggtg cgcatcgatc cgtcggtgct ggcggatccg
     1741 gaaatggccg aagacctggt cgccgccgcc ttcaacgatg cggtgaacag gatcaacgcc
     1801 gaatcgcagg cgaagatgtc ggcggccacc gccggcatgt cgctgccgcc gggcatgaag
     1861 ctgccgttct gatgacggcg ccgggacggc ccgccgccgc atcggccacc cacgcgccgg
     1921 gcggctgcgc gcgcgcgcgc ggggaagcga cgggcaaatg accgcctccc tgctcgagca
     1981 gttgatcgac gccctgcgcg tgcttcccgg ggtcggccag aagtctgcgc agcgcatggc
     2041 ttaccacgtg ctggaacggc agcgcgaggg cggccgccgg ctcgccgagg cgttggcgca
     2101 ggcggtggag cgcatcggcc actgcaggcg ttgccgcgat ttcagcgaaa ccgacctgtg
     2161 cccgacctgc agcagcggcg cgcgcgacgc gcaactgctg tgcgtggtgg aatcgccggc
     2221 ggaccggctc gcgatcgagc aggcgaccgg tttccgcggc ctctacttcg tgctgcaggg
     2281 gcgcctgagc ccgctcgacg gcatcggccc gcacgaactc gggctcgacc agctggcgca
     2341 gcggctggcc gaaggcgagg tgcaggaatt gatcgtcgcc accaacccga ccgtggaggg
     2401 cgaggccacc gcgcactacc tggcgcagct ggcgcgcgcg cacgcggtgc atcccagccg
     2461 gctggcgcac ggggtgccat tgggcggcga actggaatac gtcgatcgcg gcaccctgtc
     2521 gcatgcgttc ggcagccgca gcgaaatccc caaggagccg ccacgatgag cgacaccctg
     2581 ttccacaaga tcatccgccg cgagatcccg gcggacatcg tgtacgagga cgagcacctg
     2641 atcgcgttcc gcgacatcgc cccgcaggcg ccggtccacg tgctgttcgt gccgaaggcc
     2701 gatttcgcca ccctcgacga cgtgccggag gaacaggcgc tggtggtcgg caggctggcc
     2761 accgccgccg cggcctacgc aaggcggcag ggcttcgccg gtgacggcta ccgcgtggtc
     2821 atgaactgca accgcgatgc cggccagacc gtgttccaga tccacctgca cctgctggca
     2881 ggtgcgccgc tcgggcgctt cggcaccgcc ggctgatcgc taccagtagc cccaggccca
     2941 cggccacggc gccgggcgca ccacgacgtc gacccgttcg cgttccggcc acaggtagac
     3001 cacgtcggcg gcgaggcgcg ggaacctgta gacgtactgc tcgaccttgc gttcttcatg
     3061 gccttcgatg cggccggtga aggtgacttc gcggttcggc tggaacaggg ccgggtcgta
     3121 gaagccggtg cggcacgcga tgaagcggcc ccaggtatcg tcgctggccc aatgcgggcg
     3181 gccatcggcg ctgagcctgg tcgagatcac ctcgaagcag gtctggtccg ggcgtggctc
     3241 ggtgcggatg atgcggccgc cccagcgcac cagcgcgccg gtatggtcgc ccgccgaggc
     3301 ttcgtgcgcg gtcagcggct ggaacgtgcc ctgcagcggc tttggctggg tggcgcaggc
     3361 ggcgagcagg acgacggcga taccggcaac ggggatgcgg atgttcatgg cggctccgtg
     3421 gtgcgggaag gtggggcgga cgatgccagc gtggcatgcc acggtcgcgt cgcgttcacg
     3481 gccggtgcgc ggcgaggtcg cgcagcagtc ggcgcaactc ggccggtccg atgccagcgt
     3541 agcgcgccgc ggagaaacgc ccgctgagcg cgcgcaggtg ttcgcctgcg cgcggcacgt
     3601 cggcggcgac ccgctcggcc caggcttcgg cgggctcgtg catcccgcgc gcctggccgc
     3661 gccgcgcgta gcggcgcccg aggcgatgcc aggcgcgcag caacgggtcc tgctcgcgtt
     3721 cgccgcgggc aagcagccaa cccatccacc ccagcgcgag cgcggcgatc accgcgaaca
     3781 gcaggatcag gccgcggctg ccgatgcgct ccagcccgag cggactgagc aggcgttgct
     3841 ggcgctgcgc gttgaacccg agcacgaagt cgttccagcc gcggcgcatc cagtcgctgg
     3901 cgttgaacat cggcaccagc ccgtcgaagc cgccgatacg gccgggctgg cgatccgcaa
     3961 gggtgtcgaa cacgcgttcc ggcgcaaccg cggcggtggg atccacccgg acccagccgc
     4021 gacgcggtag ccagacctcg gcccaggcgt gggcgtctga attgagcacc agccagtaat
     4081 cgccgatcgg attgcgatag ccgccggcat agccggtgac cacccgtgac ggaatccccg
     4141 cggcacgcat cagcacgacg aaggcggagc tgaagtgctc gcaatagccc tgcttgcggt
     4201 cgaacaggaa atcgtcgatc tcgttgcgcc cggccagcgg cacgttgagg gtgtatgcga
     4261 actcgcggcg gatcatcgcc agcgcgcggt tggcgatcgc ggcgtcggcg ttgccggtcg
     4321 catcggtgcc ggcctcgcgc cgccattgcc ggccgagggc gatggtgcgc gggttgaagc
     4381 cgcgtggcag ttgcagcgca tgcatacgca gtatcggcgg caactgcgga tccaccgtcg
     4441 tcgctgcgag cgagcgcatc cgccagcggc tgacgttgtt cagcggcatc acgctgtaaa
     4501 cgctgtagtc atgactgatg aaggcgtctt tcggcacctg gtccggcagg tcgagcgtga
     4561 tgagctgcct gccttcggtg ggctcgggtt cgatgacgta atcccaagct gggcccttga
     4621 ggcgtttcgg gatcgcctgt tggaagcgcc actgcttcag ctggtgccat gtgcgtccat
     4681 cgaagtccca caagaccggg ccacgccagt acatctggtg ctgcggcggc gccgggccga
     4741 agaactgcac gcgcgcggcc ggactgtcgt cgttcatcag gtccagccag ccgcccggcg
     4801 tcatgctgtc ggacagcccc ggccgtgcga ccacgcgttc cggcagtccc cacagcggcg
     4861 tcggcaggcg cgggaacagc cagaatgccg ccagcgccag cggcaggccg agcaggacca
     4921 gcttgaacac gcccgcggtc gcctgccagg cctgcgcggc gccgggcatg cgcacctcgg
     4981 cctcgtcatg cgccagctgc tgcagggcca gcaatgccgc cagcatcgcc agcagcgcca
     5041 gcaacaggct ggccggcccc tggtcgagca ggaatgcggc gaacggcgcg aacagcgcga
     5101 aaccgaccag gctccggccg tcgcgcaggc tgacggtttc cgagggtttc agtgccagca
     5161 tcgcggccag caccgcgcat gcggtgtcgc ggcccacccc gggtgcgacc gcggcgatcg
     5221 cgacgatcat gccgatgccg accagcaggc gcagcagcat cggcggcacc cgccgccacg
     5281 acaccgcggc gaccagcacc gcggcgaccc cgaacccgat cccgagctcg cgcggcagct
     5341 gcagcagcag cggcagcagg caggccgcgg tcgccagcag caccagcgcg cggccgcgca
     5401 cctgcatcgg cggcgacttc aagcgcgcca gcaatgggcg gaactcaggc atgcgcggcc
     5461 tcgcccggca tcagcgccag cgcgcgcagg cagcggtggc ggtgggccat gcccaggccc
     5521 atcggcaccg gcgcctgtcc gggcaggccc agcgcatagc ggcggccctg gcgctcggca
     5581 tcgtccaccc agcgcgccag ccggcggatc cgctgttcgt agggcaggcc ggcggtgagc
     5641 ggccagtcga gcagcaactg gtcgctgcga tgctgctcgt actcgcgcac cagcaggctg
     5701 tcgcggcgcg ccgacgcctt ccatgccacc gaacgcgggg catcgccggc acggtagcca
     5761 cgcaggtggt ggacatcgtc gccggcgcgg gtgacctgcg cgcggccggc gtcgccggca
     5821 ggtgccggca gcggcggtgc ctgcatttcc ggtgccgggt acaccagcgc gacctgccgt
     5881 ggccacacgt aggcccaggc gcgcgccagc cccagcggct gcagcgtgcg cagttccagc
     5941 cgcggcaacg gcatcaggcc gcggcgcccg gtcgggatcg gcagttcgac ggtgccgcca
     6001 tggtggtcga acgccgcgtg cacgtgggcg tcgccgtggg agatacgcag gccgcgacgc
     6061 atgcgctggt cgcgcgcgcc cagcgaaatg cgcagccgca gcggttcccc ggccggtacc
     6121 gggtccgccg ccaccgcatc caccaccacc ccggacaact gcatgtgcgc cgcgatcagg
     6181 ctggcctgcg cggcgccggc cagcagcagt gccagcagca gtcccgggtt gttgttgaag
     6241 ttgagcgcgc ccaggcccat ggtcccgagc agcagcgcga agaacaggcc ggtcgcggtc
     6301 ggcagcacgt agatgcggtt gcgccgcagg cgcaccggca gggattcggc ctggcgcggc
     6361 cgcatccacg actgcaggcg tcggcgcagc caggcgatca tcggtcagtc gaccggcacg
     6421 gcgtgcacga tcgccttcgc cagcgccgcc gccgaggtgt ccgcctcgcc gaccagccga
     6481 tgctcggcga ccgccgcgaa cagcgcctgc acgtcctccg ggaccacgtg gccacgaccc
     6541 agcagcagcg cgtgcgcgcg cgccgcgcgc agcagcgcca gcccggcgcg cggcgacagg
     6601 cccacgcgca cccccggatg cttgcggctg cgcccgacca gcgcctgcac gtagccgacc
     6661 agcgcctcgc tcgcgtgcac cgccagcacc gcccgccgca gcgcgatcac gcgttcggca
     6721 tccagcagcg gcgtggcctg cgcgatcagg tcgcggcggt cgctgccggc cagcagcgcg
     6781 cgttcggcct gctcgtccgg atagcccagc gccagccgca gcaggaaccg gtccagttgc
     6841 gaatccggca gcggataggt gccggccagg tcgaccgggt tctgggtcgc gatcaccagg
     6901 aacggatcgg gcagggcgcg ggtggcgccg tcgatgctga cctggtgttc ggccatcgct
     6961 tcgagcaagg cgctctgggt gcgcggcggc gcgcggttga tctcgtcggc cagcaggaca
     7021 tgggtaaaca ccgggccggg atggaattcg aaccgccgcg cctgcgcctc gtacaccgac
     7081 accccgagga tgtccgccgg cagcaggtcg gaggtgaact gcacgcgctg gaaaccgagg
     7141 ccgagggtgg ccgccatcgc atgcgccagc gtggtcttgc cgaggccggg caggtcctcg
     7201 atcagcaggt ggccatcggc gagcagggcg acgaaggcca gccgcacctg ctgcggcttg
     7261 cccagcacca gtgtgttgac ctgcgcctgg gcatcgcgca gggcgacgcg cagggcttcg
     7321 ggtagcatcg tgtcggtggc cgggggtgcg gacatggcgt acttgcctgc gggcaagcct
     7381 ctggcgggtt tccctttgta gtgtagcgag gcggcgaacg gatgcagggc gtgcaggaaa
     7441 cggacttcgc caagcgctgg ttcgtcggtc cgtggctggc cttgctcggc ctgaagctgt
     7501 ggctggcgtc gcggctgccg ctgttcgtcg acgaagcctt ctactggcag gaaggccgcc
     7561 accttgcatg ggcctattcg gatttgccgg gcctgaccgc atggctggcg cgcatcggcg
     7621 acggcttggg cgatggcacc ctggccctgc gcctgccgtt cctcgccatc gccgcggcga
     7681 tcccgtggct ggtcgtgcgg ctggccgcgc gcgagctgga tccgaaggca ggctggcagg
     7741 ccggcaccct ggccttgctg ttgcccctgg ccgggaccat gggcctgctg gccgtgcccg
     7801 acgtgccgct gttgctggcc acgctgctgt gcctggatgc cggcgcgcgc ctgttgcggc
     7861 gcgtggacgc attcgcggca cttgaactgg cactgggcct ggtcatcggc ggcctcaccc
     7921 attaccgctt cgccgcggtg atcgcggtcg gcttctgcgc gttgctggtg ctgcgcgacg
     7981 ggcgcgcggc gttgcgcgac cgacgggtgt ggatcgcgct ggccgccggt gcgctggcgt
     8041 ggctaccgct ggtgctgtgg aacctggaca acgccgatgc cggcctgcgc ttccagctgg
     8101 tcgaccgcca tccgtggagc ttcagcggcg cgggcgttcg cctcgggcgc atgcaactgc
     8161 tgctggcgac gccgctgctg ctgctggcga tggtggttgc ggcgtggcgt ggctggcgcg
     8221 atggcaggcc cgccgcccgc tacctggccg ccagcggctc gatgatcgtg cttggcttcc
     8281 tgttgctcgg cttcttcgcc gaccgcgagc gggtcagctt ccattggccg ctgccgggct
     8341 acctggcgct gttgccgctg gtgccggcgg tgctcgcggg ctggccacgg ggttggcgca
     8401 tcgccacctg ggcgaccgca gcagtcggtc tgggcgcggt gctgggctac tacgcggtcg
     8461 catcggtgcc gtcgtggcgc gcgcaaaccg cctcgcggaa tttccatccg accaatttcg
     8521 ccggctggcg ggaactggat gcggcggtcc gcgacgccct ggcgacgatg cccgcggaca
     8581 cccgcctgct tgccggcaac ttcaagatcg gcgccgaact gggcttcgcc cgcgacgatg
     8641 cgggcatcgc ggtgctcgag cacccgctca acgacaagca cgggcgggca ccgcaactcg
     8701 cgttgtgggg gatctcgcac gcaagccgcg ccaccctggg cgacggtccg ctgctgctgg
     8761 tcgccagcag cagcgacgcc cgcttccgcg agctgctcgc gcattaccac gcgctgtgcc
     8821 ggcaagttgg cccgctgccg ccgccacggg tcctcaacat cgaccacggc cgccagcgtt
     8881 tcctgctgtt cgcgatcccg cgcggtatcc gctcgggcgc gtgcaccgcg cccgcgctcg
     8941 ccttcctcga tgcgccggtg gcggggaagg ccgttgccgg tggcttcgag gtctccggct
     9001 gggcgttcaa ggacggcgtc ggcctgcgcg gggtcgaggt gatgctggat ggccgcgtgg
     9061 tcgcgcaggc gcgctatggc gagacgaatc cgggcgtcac cggctactgg aaggacagca
     9121 acgatcgcaa ccacccgaac gtgtatttcc gtgcgcgcgt cgagggtgcg cagccgggcg
     9181 cgcgctggct ggggctgcgc ctgcatggcg ccgatggcag cgtcgaggac tggccggagc
     9241 agaagatcga ggtgcgttga gcgaaggcct cagggcagcg cgaagccggc tgcgcgcagc
     9301 agccggcagg tcgcgatcag cggcaggccg atcagtgcag tcgggtcgcg gttgtcgatc
     9361 cgctcgaaca gggcgatgcc cagtccctcg gacttgaagc tgcccgcgca atcgaacggc
     9421 tgttctgcat cgacgtagcg ttcgatctcg gctgcctcca gcacgcggaa atgcacctcg
     9481 gtcaggtcgc agccggcatg caggcggcca tcggcgtggg ccaggcacag cgcggtgtgg
     9541 aagcgcacca cgcgacccga catcgaatcc agttgcgcga tcgccgcgtc gcggccgccg
     9601 ggcttgccca gcgcgcggcc ttcgacctcg gccacctggt cggaacccag cgcccaggta
     9661 tcgccgccga gttgcagcgc caccgcccgg gccttggcct gtgccagccg cgccgcgagc
     9721 atgccgggcg cctcgccggg cagcgcggtt tcgtccaccg cggggctggc gacatcgaag
     9781 gccaggcgca gtcgttgcag caactcgcgc cggtagcgcg agctcgacgc cagcaccagc
     9841 cgcgccacct tcagtgcccg gcgcgggctt gcgagacccg ctgcagctgc tgctccaggc
     9901 tggtgcgcgc gctttcaagg gtgcgccgga ccgcgtccag ttgcgcaagg tcggcggatc
     9961 cggcgttgtc ctgcagccac aggcgctggc gcaggatttc ctgcatggcg ccgcgcaccg
    10021 ggttctcgtg gtcgcccgcc accgcttcca gctcttcgcg ggcgacccgc aaccgcgctt
    10081 ccagtgcgtc ggcggcatcc agcacgccgc ggatcgcgcg ctggcggccc tgtccgcgtt
    10141 ggcgggctcg ccacgacagc catgcgacga cggcgagggt ggcggccagc agggcgggca
    10201 ggacgaggtt catgcgggca gtttgcgcga gcgccggccg cgcggcaaac cgggcgccgg
    10261 cgcggcgcgt gcggatcgct gcatcgcatt gatttgacag gaaacccgcc ggtctttacc
    10321 attctgcggc ttatgtccga cccttcgatc agccgggtgc cagagtccgt ggatgcctgg
    10381 cggatggtcg cggcgcagcg tgaattcgaa ggccggattc cgctcgcgtc gatggcccgg
    10441 ttgcgcgacg gcctgcttga acccgaaggc gacgcccgct tcacgctggc gttcggaacc
    10501 gatgcgctgc gcttgcctta cgtggagctg cgggtcgaag ccgagttgcc gctggagtgc
    10561 cagagcagcc tgcaacgttt cctgctgccg gtgcaactgg tgcagcggct gggcctggtc
    10621 cgcgacgagg ccgatgaagc ggcgttgccg gaagggtacg aagccctgct ggtcgatgcg
    10681 gacggtgagc tgaagccggc ggagctggtg gaggacgagt tgatcctggc cttgccggtg
    10741 gttgcggtgg cgcccggggc cgaggtggtc gagcgcgaat tcgtgccggg cgccgaggaa
    10801 accgccgagg ccaacccgtt cgcggcgttg gcgggcctga agaaaagata agtgcgataa
    10861 tgtcgcgctg atttgcagta acgagctcta cgagcattca ttcggagttg tcccatggct
    10921 gtccagaagt cccgcgtttc cccgtcccgc cgcggcatgc gccgcgcgca cgacgccctg
    10981 tccgccaagc agctggccac tgatccgacc agcggcgaga cccacctgcg ccaccacgtg
    11041 accgccgacg gctactaccg cggcaagcag gtcatcgcgc cgaagaccca ggtcgccgac
    11101 gaagagtgat ccgcggccgc aaggtcgtag cagcaggatg acgcccggcc gcgccagatg
    11161 gtgcggccgt tggcgtttct gcatccgcga atcatcggtg gccgcaaggc gctgctcgcc
    11221 accgcccgcc cgcgctatgg tggcgggcca gccaggagtc cacgcatgag tcagtcccaa
    11281 ggccgaatct tttcccgcat cgccggcacc ggcagttacc tgccggaaaa ggtactgacc
    11341 aacgccgacc tcaccgagtt cgtcgagacc accgacgagt ggatcgtcgc ccgcaccggc
    11401 atccgcgagc gccatgtcgc ggccgagggc gagaccacct ccgacctcgc ctaccatgcc
    11461 gccgtgcggg cgatggaggc ggccggcgtg accgccgccg acatcgacct gatcgtgctg
    11521 ggcaccacca ccccggacct gatctttccg tcctcggcct gcctgctgca gcacaagctg
    11581 ggcgccaacg gctgcccggc cttcgacgtc aacgcggcct gttccggctt catctacgcg
    11641 ctcaccatcg ccgacaagtt catccagtcc ggtgccgcga agaccgcgct ggtggtgggt
    11701 tcggaaaccc tgacccggat gctggactgg agcgaccgcg gcacctgcgt gctgttcggc
    11761 gacggtgccg gcgcggtggt gctgaaggcc gacggcgaaa ccggcatcct cagcacccac
    11821 atgcatgccg atggcggcaa gaaggaactg ctgtggaacc cggtgggcgt ttcggtcggc
    11881 ttcaagccgg acgagaagaa cgccggcgta cgggtgctga tgaccggcaa cgaggtgttc
    11941 aagcatgcgg tgaaggcgct ggactcggtg gtcgaggaag cgctgctggc gaacgggctg
    12001 gaccgccacg aacttgactg gctggtgccg caccaggcca acctgcgcat catcgaggcc
    12061 accgccaagc ggctggagat gccgatggag cgtgtcatcg tcaccgtcga caggcacggc
    12121 aatacctcgt ccggctcggt gccgctggcg ctcgacgagg cggttcgcgc cggcaagatc
    12181 cagcgcggcc aactggtcct gctggaggcg ttcggcggcg gcttcacctg gggctcggcg
    12241 ctcctgcgct attgaggttc caagcggtcg cgcaccaggt cagtcagtac gccgccccga
    12301 tgcgtgcgaa catcctgttc gacgcatcgt cgcaggccat ccatggcctg cttgcggtcg
    12361 taccgcctgt ccggatccgc gccctgcagg ttcaagcccg catacgcccc ggtacagacg
    12421 cgctagagca attgctcgta tcatgcgcgt cccttgatat cgcgggcgtt cccacgcccg
    12481 atgccgcgtg agcgaaaccc ccgcacaact cgccctggtg tttcctggcc agggctcgca
    12541 gtcggtcggc atgctggccg aactctcgga actgcatccc tcggtgaagg cgaccttcga
    12601 ggaagcctcc gacggtgccg gcgtggacct gtgggcgctg tcgcaggccg gcccggagga
    12661 aatgctcaat cgcaccgaat acacccagcc ggcgctgctg gccgccgggg tggcggtttg
    12721 gcggctgtgg caggcgcagg gaggcgcgca gcccgccgtg ctggccggcc acagcctggg
    12781 cgaatacacc gcgctggtcg cggccggcgc gctttcgctg cgcgatggcg cgcacctggt
    12841 gcgcatccgc ggccagctga tgcaggacgc cgcgccggcc ggcgtcggcg cgatggcggc
    12901 ggtgctcggg gccgaggacg cgctggtcga agcggcctgc gtggaagcgt cgggcgcgca
    12961 ggtggtggtg ccggccaact acaactcgcc gggccaggtc gtgatcggcg gcgatgcggc
    13021 ggcggtggat cgcgcgctgg cgctgctgca ggaaaaaggc gtgcgcaagg cggtcaagct
    13081 ggcggtcagc gtgccgtcgc acacgccgct gatgcgcgaa gcggccaatc ggctggcgaa
    13141 caccatggcc ggcctggcct ggcggttgcc ggcgctgccg gtggtgcaga acgtggatgc
    13201 ccgcgcgcac gacagcgtcg atgcgatccg cgacgcgctg gtgcgccagc tctacctgcc
    13261 ggtgcaatgg ac
//
LOCUS       DAORUK010000053        12802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_4877_length_12802_cov_19.807641, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000053 DAORUK010000000
VERSION     DAORUK010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 12802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12802
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4877_length_12802_cov_19.807641"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..2385)
                     /locus_tag="PK818_05215"
     CDS             complement(124..2385)
                     /locus_tag="PK818_05215"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HQY82168.1"
                     /translation="MLEQHGLLLALVCAVIAIAYGLWAAGWINRQPAGNERMREIAGA
                     IQEGARAYLNRQYTTIAIAGAVLFVLIGVFLSWLTAIGFLIGAVLSGAAGYIGMNVSV
                     RANVRTAEAARSGMSQAMDVAFKGGAITGMLVVGLGLLGVAGYYGLLTKYMGASEKDA
                     LHALVGLAFGASLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNV
                     GDNVGDCAGMAADLFETYAVTIIATMLLGSLMVATAGKNAVLYPLVLGGVSIIASIIG
                     AFFVKVGGDKPNIMGALYKGVIVSAVLAAVAFYPITTQLMADSSHGAMNLYWCALIGL
                     VLTGAIVWITEYYTGTQYKPVQHVAQASTTGHGTNIIAGLGVSMKSTALPVIAVCASI
                     LLAYKLGGLYGIAIAATSMLSMAGMIVALDAYGPITDNAGGIAEMAELPPEVRNITDP
                     LDAVGNTTKAVTKGYAIGSAALAALVLFADYTHNLQTQHPGVNFTFDLSNHMVIVGLL
                     IGGLIPYLFGAMAMEAVGRAAGSVVEEVRRQFRDIPGIMEGTGKPDYSRAVDMLTKSA
                     IKEMIVPSLLPVAVPVVVGLTLGAQALGGLLIGTIVTGIFVAISMTTGGGAWDNAKKY
                     IEDGHHGGKGSEAHKAAVTGDTVGDPYKDTAGPAINPLIKIINIVALLLVPLLPATLT
                     SGESLQAPEAIEAPAATMDAAPMPAEAPAADTAAPMPEESPAIEAPAMDPASPAPAEA
                     PASTAEAPEPATN"
     gene            2605..3120
                     /locus_tag="PK818_05220"
     CDS             2605..3120
                     /locus_tag="PK818_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82169.1"
                     /translation="MLRPHMLFPGLLAVAFGAVAGEPTPEIQRKPGAPQAIGVAHTLR
                     TIPEACARLEGRFTGDAAAPYRFTAVRSSARCQPRARLVDAAAAKPSAASGWVLNDVV
                     RVPNAGCPSQQAVVRVWRKDAKAVPPKLDAQGRSRIYLKEGLDTARGGDLGPIPMYAA
                     AMDIEGKDCGK"
     gene            complement(3128..3352)
                     /locus_tag="PK818_05225"
     CDS             complement(3128..3352)
                     /locus_tag="PK818_05225"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HQY82170.1"
                     /translation="MPVIQVKAKPNARASSLTRQDDGTWLAQLKSAPVDGKANAELVE
                     LVAREFGCAKSSVTIKAGAGSKFKRVVVPD"
     gene            complement(3425..4225)
                     /locus_tag="PK818_05230"
     CDS             complement(3425..4225)
                     /locus_tag="PK818_05230"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HQY82171.1"
                     /translation="MKQHLLIAGLCMLLATPAQAQWNKKQNDPQGAAAGANAKGAGLN
                     LEYCDEPMGTIAVVEEQEGDWYRYLSSDLRLPSTVPVIRMLIQQSNCFVVVERGRAMQ
                     NMMQERALMDSGELREGSGFGKGQMVAADYTMNPSINFSQSNAGGVGGVLSAFGGRHG
                     MVAGALVGGLKFKKAETMLTMIDNRSGVQIGVAQGNATKTDFSLGIGGGGGGGFGALG
                     GYSNTPEGKALAAAFANAYNNLVVAMRDYQPQQVQGGLGKGGKIKVGD"
     gene            complement(4301..5557)
                     /locus_tag="PK818_05235"
     CDS             complement(4301..5557)
                     /locus_tag="PK818_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019338784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HQY82172.1"
                     /translation="MPAGTLLYAQSGGVTAVINATASAVIQSARARKVRVLAARNGIL
                     GALREELIDTTPLTAAQVRALAHTPGGAFGSCRLKLKSLAGDRAKYERLLAVFKAHDV
                     RWFLYNGGNDSADTALKVSQLAAEFGYPLTCIGVPKTIDNDLAMTDCCPGFGSAAKYT
                     AVSVRECALDVAAMADTSTKVFVYEAMGRHAGWLAAAAGLAGAGKDDAPHLILFPERP
                     FDEAGFLARVDAVVKRVGHCVVVASEGIRSADGRFVADAGGGKDSFGHTQLGGVASYL
                     AGRVKDALGHKVHWALPDYLQRSARHIASQVDLEHALAVGRAAVQCALAGQNAVMPVI
                     VRDADAPYRWHVDIAPLERVANHEKTMPKGFIRKDGYGITAAARRYLQPLIEGEAFPP
                     FGRNGLPDYVQVDQAVLRRTLPAWQG"
     gene            5689..6267
                     /locus_tag="PK818_05240"
     CDS             5689..6267
                     /locus_tag="PK818_05240"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HQY82173.1"
                     /translation="MRLVLLGAPGSGKGTQAARLKEHLQVPHISTGDLLRAEVAAGSP
                     LGLQAKEVMARGDLVSDDILLGMLRDRFSRDDTRAGFILDGYPRNLAQAAALDKLLAE
                     LGQAFDAAVQLTVDNEQIIERLAGRAKAEGRADDTPESVRHRLNVYDQQTAPVIDFYR
                     QHGQLTVVDGVGSLDEVFTRILEAIQPGHEVG"
     gene            6383..7744
                     /gene="mpl"
                     /locus_tag="PK818_05245"
     CDS             6383..7744
                     /gene="mpl"
                     /locus_tag="PK818_05245"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HQY82174.1"
                     /translation="MKLHILGIAGTFMGGVAALARELGHEVEGSDQNIYPPMSTQLER
                     LGIALKPGYEAANISADCDEIVVGNALSRGNEAVEHVLDAGLRYTSGAQWLSERLLPG
                     RDVLAVAGTHGKTTTTTILSYLLERAGRAPGFLIGGVAEDFGVSARIGGGREFVVEAD
                     EYDTAFFDKRSKFVHYRPLVAILNNLEYDHADIFPDVAAIQRQFHHLVRTVPRRGRLL
                     VNGHDRHLREVLAMGCWTPVERFGFDPSFEWSARKLRDDGSAFVVLHAGQELGTVEWP
                     MLGDHNVLNGLAALAACSAVGVDVASVIPSLAGFSSVKRRMEVIGEHDGITVYDDFAH
                     HPTAIQTTLAGLRAKVGAARIVVAMEPRSNSMRLGAHAQALAPSLDIADVVVFLARPE
                     LPWDADQVIAAIRGDARAVADADALVARLREIARDGDHVVFMSNGGFDGAPRRFLAAL
                     RDA"
     gene            7737..8330
                     /locus_tag="PK818_05250"
     CDS             7737..8330
                     /locus_tag="PK818_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HQY82175.1"
                     /translation="MPEAGAPLPLLPLQAVLVPGAGLDLRIFERRYLDMVRDCGRSGS
                     GFGVCLILEGSETGAAATPAAFGTEALIEDFGSTPDGLLSLRVRGRRRFHVLRTRVRD
                     NGLLVADVEWREDAAGSPRLRPEHALLAELLRRILLQVAGEHAEPAPLLFESAAWVGW
                     RLAELLPLEMRQRQALLQDDDPHARLQRLLEAIAAEG"
     gene            complement(8335..10266)
                     /locus_tag="PK818_05255"
     CDS             complement(8335..10266)
                     /locus_tag="PK818_05255"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DedA family/phosphatase PAP2 family
                     protein"
                     /protein_id="HQY82176.1"
                     /translation="MQSAWFDATIAWISAHPHAAGGLVFLIAFCDALAVVGIVVPALP
                     LLFAVGTLIGLGHLDGPYALLCAAGGAFAGDGLSYWIGYRWGPAMRGLWLFRRYPGLL
                     DRGEQMFRRHGAKGIVTARFVGAVRPFVPAVAGMLHMPLKRYAPASAVAAFAWAAVFL
                     APGWTFGASYDAVAAVADKLVLVLLGLAAALALGWALVLYTWRWFAAHADNLLARALR
                     WTRAHPRLGRYAAALIDPRRPESASLVVLATCLFTVAWLWAGFTTWLLVRGEPLLFDR
                     ELQATMFALRNPLADRLMADLAAIGSAPVLGTASVAALLWLGWRRRWMAAAHWLAAIA
                     VGLLLTVGLDALVDTPRPPTAAAGFGFPSIAVTMTTVVFGFFAVLIAREFPGRQRVWP
                     YLMAGVATVLVAFARLYLGAHWLSDVIAGVLLGVVWVLLIGIAYRRHSTRSFWMRPLA
                     WMFYGGFALALGWYAPRSADPTLARFTPPAPQQRLALVQWQAHGIPDPRHFDLQAAGD
                     LRPLQQRLQAQGWRVQPQADWISVLGLLDDDRSLARKPVLPLALDAHPERLLLRRTGN
                     DPRWIEVLRIWPAPAQLDDGTALWVGRYERMHAHTRLRLLTLWHPVPQAGTLPADLQA
                     LAARAAVADPRVQVRVDTR"
     gene            10340..11158
                     /locus_tag="PK818_05260"
     CDS             10340..11158
                     /locus_tag="PK818_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005419027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY82177.1"
                     /translation="MATLSGKTLFITGASRGIGLAIALRAARDGANIAIAAKSAVANP
                     KLPGTIHSAAAQVEAAGGRALALKCDIREEDEVRAAVAATVDAFGGIDILVNNASAIW
                     LRGALDTPMKRFDLMQQVNSRGSFLCAQACLPHLLQAPNPHILTLCPPPSLDPKWWGP
                     HAGYTLAKMGMSFVTLGLAAEFGPQGVAVNALWPRTVIATDAINMIPGVDARNCRTPQ
                     VVADAAHAVLVRAARGFHGKFLIDEDVLREAGCTDFDRYAVEPGQRLLPDLFLE"
     gene            11162..11593
                     /locus_tag="PK818_05265"
     CDS             11162..11593
                     /locus_tag="PK818_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY82178.1"
                     /translation="MRYLHTMVRVRDLEANLRFFRDGLGLRETRRMENPQGRYTLVFL
                     AADETPDAEIELTYNWPDAGGAIEDYGSARNFGHLAFRVDDIYATCAHLQAMGYTINR
                     PPRDGHMAFVRSPDLVSVELLQDGHLPPREPWASMPNTGAW"
     gene            11599..>12802
                     /locus_tag="PK818_05270"
     CDS             11599..>12802
                     /locus_tag="PK818_05270"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HQY82179.1"
                     /translation="MDRADLLALGRERYLPVYRPRELILERGQGARLWDSDGRDYVDF
                     GAGIAVCSLGHCDPELVAALQEQAGKLWHTSNVFYSEPPLRLADELVVASRFAERVFL
                     CNSGTEANEAAIKLVRKWASAQGRAPDRRVILSFRGSFHGRTLAAVTATAQPKYQEGY
                     EPLPGGFRYTGFNDLEAVEQAMAAGDVAAILVEPVQGEGGVMPAAPGFLAGLRALCDA
                     HGALLVLDEIQCGMGRTGSLFAHWQDEATPDIVTLAKALGGGFPIGAMLAGPQVAQAM
                     QFGAHGTTFGGNPLAAAVARVALRRLASDGIAANVRRQERAIRDGLDAINRDLDLFAE
                     VRGRGLMLGAVLKPEHAGQASAILDAAADHGLLLLQAGPDVLRFVPALNIGDDEVADG
                     LARLRAALE"
BASE COUNT         1916 a         4490 c         4505 g         1891 t
ORIGIN      
        1 gggcgcgcat tctaaccggt gggggcgggc cggtttcccg ggcgcggccc gcggccatgc
       61 ccgcagcaac gaaaacggcg gccgaagccg ccgtttgcgt ggagcgaagt cgcggttgca
      121 gggtcagttc gtcgccggtt ccggcgcctc ggcggtcgag gccggggctt cggcgggcgc
      181 cgggctggcc ggatccatcg cgggcgcctc gatcgccggc gactcctccg gcattggcgc
      241 ggcggtatcc gcggccggcg cttccgccgg catcggcgcc gcgtccatgg tggccgccgg
      301 ggcttcgatc gcctccggcg cctgcaggct ttcaccgctg gtcaaggtgg ccggcagcag
      361 cggcaccagc agcagggcga cgatgttgat gatcttgatc agcgggttga tcgccgggcc
      421 ggcggtgtcc ttgtacgggt cgccgacggt gtcgccggtg accgcggcct tgtgcgcctc
      481 ggaacccttg ccgccgtggt ggccgtcctc gatgtacttc ttggcgttgt cccaggcgcc
      541 gccgccggtg gtcatcgaga tcgccacgaa gatgccggtg acgatggtgc cgatcagcag
      601 gccgccgagc gcctgcgcgc ccagggtcag gccgaccacc accgggaccg cgaccggcag
      661 cagcgaaggc acgatcattt ccttgatcgc ggacttggtc agcatgtcca cggcgcggct
      721 gtagtccggc ttgccggtgc cttccatgat gccggggatg tcgcggaact ggcggcgcac
      781 ttcctccacc accgaacccg ccgcgcggcc cacggcttcc atcgccatcg cgccgaacag
      841 gtacgggatc aggccgccga tcagcaggcc gacgatcacc atgtggttgc tcaggtcgaa
      901 ggtgaagttc acccccgggt gctgggtctg caggttgtgg gtgtagtcgg cgaacagcac
      961 cagcgcggcg agcgcggcgg aaccgatcgc gtagcccttg gtcaccgcct tggtggtgtt
     1021 gcccaccgcg tcgagcgggt cggtgatgtt gcgcacttcc ggcggcagtt ccgccatctc
     1081 ggcgatgccg ccggcgttgt cggtgatcgg gccgtacgcg tccagcgcca cgatcatccc
     1141 cgccatcgac agcatcgagg tggcggcgat ggcgatgccg tacaggccgc cgagcttgta
     1201 ggccagcagg atcgaggcgc acaccgcgat caccggcagc gcggtcgact tcatcgacac
     1261 gccgagcccg gcgatgatgt tggtgccgtg gccggtggtc gaggcctggg ccacgtgctg
     1321 cacaggcttg tactgggtgc cggtgtagta ctcggtgatc cagacgatgg cgccggtcag
     1381 caccaggccg atcagcgcgc accagtacag gttcatcgcg ccgtggctgg aatcggccat
     1441 cagctgggtg gtgatcgggt agaacgcgac cgccgccagc accgccgaca cgatcacgcc
     1501 cttgtacagc gcgcccatga tgttgggctt gtcgccgccg accttgacga agaacgcgcc
     1561 gatgatcgag gcgatgatcg acaccccgcc cagcaccagc gggtacagca ccgcgttctt
     1621 gccggcggtg gcgaccatca ggctgcccag cagcatggtc gcgatgatgg tgaccgcgta
     1681 ggtctcgaac aggtcggcgg ccatgccggc gcagtcgccg acgttgtcgc ccacgttgtc
     1741 ggcgatcacc gccgggttgc gcgggtcatc ctcggggatg ccggcttcga ccttgcccac
     1801 caggtccgcg ccgacgtcgg cgcccttggt gaagatgccg ccgccgaggc gggcgaagat
     1861 cgagatcagc gaggcgccga aggcgaggcc gaccagcgca tgcagggcgt ccttttcgct
     1921 ggcgcccatg tacttggtca gcagcccgta atagccggcc acgccgagca ggccgaggcc
     1981 gacgaccagc atgccggtga tcgcgccgcc cttgaaggcc acgtccatcg cctggctcat
     2041 gccgctgcgc gcggcctctg cggtgcgcac gttggcgcgc accgacacgt tcatgccgat
     2101 gtagccggcc gcacccgaaa gcactgcgcc gatcaggaag ccgatcgcgg tgagccagct
     2161 caggaacacg ccgatcagca cgaacagcac tgcgccggcg atggcgatgg tggtgtattg
     2221 gcggttgagg taggcgcgcg cgccttcctg gatggcgccg gcgatctcgc gcatgcgctc
     2281 gttgccggct ggttgcctgt tgatccagcc cgcggcccac aggccatagg cgattgcgat
     2341 gacggcgcag acaagcgcca gcaataaacc gtgttgctcc agcatgaacc cctccccgga
     2401 gtctttcgaa ggaatgaacg gacactgatt gagtgcgtgc ctgcggtgca ggcaacctgc
     2461 ggactatcgc acagccatgc gcgccgtgga cgcgcggcga agcaggaacc gcgccatgaa
     2521 tccggggttc aggcccgccg tgccagcctg cggcctgtcc ggatgccggt ttcggcgccg
     2581 gtcctggagc tggaggacgt cccgatgctg cgaccgcaca tgctgttccc gggcctgctg
     2641 gcggtcgcct tcggcgcggt cgccggcgag ccgacgcccg agatccagcg caagccgggg
     2701 gccccgcagg cgatcggggt ggcgcacacc ctgcgcacca tccccgaggc ctgcgcgcgc
     2761 ctggaaggcc ggttcaccgg cgacgccgcc gctccgtacc ggttcaccgc ggtccgcagc
     2821 agcgcgcgct gccagccacg cgcgcggctg gtcgacgccg ccgcggccaa gccttccgct
     2881 gccagcggct gggtcttgaa cgacgtggtc cgggtgccga acgcgggctg cccgtcgcag
     2941 caggcggtgg tgcgggtctg gcgcaaggac gcgaaggcgg tcccgccgaa gctcgatgcg
     3001 caggggcgtt cgcgcatcta cctcaaggag gggctggata ccgcgcgtgg cggcgacctc
     3061 ggcccgatcc cgatgtacgc ggctgcgatg gacatcgaag gcaaggactg cggcaagtga
     3121 tgccggatca gtccggcacc accacccgct tgaacttgct gccggcgccg gccttgatgg
     3181 tcacgctgga cttcgcgcaa ccgaattcgc gcgcgaccag ttcgaccagc tccgcgttcg
     3241 ccttgccgtc caccggcgcc gacttcagtt gcgccagcca ggtgccgtca tcctgacggg
     3301 tgagcgaaga cgcgcgcgcg ttcggcttgg ccttgacctg gatgacgggc atgggtgcag
     3361 tgtgccggat gcacgaacgc ccggcattgc cgggcgtccg tgagcttcgc gattgcaccg
     3421 ttggtcagtc gccgaccttg atcttgccgc ccttgccgag gccaccctgg acctgttgcg
     3481 gctggtagtc gcgcatggcg acgaccaggt tgttgtaggc attggcgaac gccgccgcca
     3541 gcgccttgcc ttccggcgtg ttgctgtagc cgccgagtgc gccgaaaccg ccgccgccgc
     3601 caccgccgat gccaaggctg aaatcggtct tggtggcatt gccctgggcc acgccgatct
     3661 gcacgccgga gcggttgtcg atcatggtca gcatggtttc ggccttcttg aacttgaggc
     3721 cgccgaccag ggcgccggcg accatgccgt gcctgccgcc gaacgcgctc agcacgccgc
     3781 ccacgccgcc ggcattgctc tgcgagaagt tgatgctggg attcatggtg tagtcggcgg
     3841 cgaccatctg gcccttgccg aagccgctgc cttcgcgcag ttcgccgctg tccatcagcg
     3901 cccgctcctg catcatgttc tgcatcgccc gcccgcgttc gaccaccacg aagcagttcg
     3961 actgctggat cagcatgcgg atcaccggca ccgtcgaagg caggcgcagg tcgctgctga
     4021 ggtagcggta ccagtcgcct tcctgttcct cgaccaccgc gatggtgccc atcggttcgt
     4081 cgcagtactc gaggttgagc ccggcaccct tggcgttggc gccggcggcg gcaccctgcg
     4141 ggtcgttctg cttcttgttc cactgcgcct gcgccggcgt ggccagcagc atgcacaggc
     4201 ccgcgatcag aaggtgttgc ttcatggagt atccccggat tcgtgttcgt tggtccccgc
     4261 cggccgcgcc ggacggggtg attgtagcgg cgacgcgcgc tcagccctgc caggcgggca
     4321 gcgtgcgacg caggacggcc tggtctacct gaacgtaatc cgggagccca ttccggccaa
     4381 acggcgggaa tgcctcgccc tcgatcagcg gctgcaggta gcgccgggcg gcggcggtga
     4441 tgccgtagcc gtccttgcgg atgaagccct tcggcatcgt cttctcgtgg ttggcgacgc
     4501 gctccagcgg agcgatgtcg acgtgccagc ggtagggggc atcggcatcg cggacgatca
     4561 ccggcatcac cgcgttctgc ccggccagcg cgcactgcac cgcggcgcgg ccgaccgcca
     4621 gcgcatgttc caggtccact tgcgaggcga tgtggcgggc cgagcgctgc aggtagtccg
     4681 gcagcgccca gtgcaccttg tgccccagcg catccttgac ccggccggcc aggtacgagg
     4741 ccacgccgcc cagctgggtg tgcccgaacg aatccttgcc accgccggca tccgccacga
     4801 agcggccgtc agcgctgcgg atgccctcgc tggcgaccac cacgcagtgg ccgacccgct
     4861 tcaccaccgc atcgacccgg gccaggaacc cggcctcgtc gaacggacgc tccgggaaca
     4921 ggatcaggtg cggcgcatcg tccttgcctg cgccggccag tccggcggcg gcggccagcc
     4981 agccggcatg ccggcccatc gcctcgtaca cgaacacctt ggtcgaggtg tccgccatcg
     5041 ccgccacgtc gagcgcgcat tcgcgcaccg acaccgcggt gtacttggcg gccgagccga
     5101 agccggggca gcagtcggtc atcgccaggt cgttgtcgat ggtcttgggc acgccgatgc
     5161 aggtcagcgg atagccgaat tccgccgcca gctgggacac cttgagcgcg gtgtcggcgg
     5221 aatcgttgcc gccgttgtag aggaaccagc gcacgtcgtg ggccttgaac accgccagca
     5281 ggcgctcgta tttcgcgcga tccccggcca gcgacttcag cttgagccgg caactgccga
     5341 acgcaccgcc cggcgtgtgc gccagcgccc gcacctgggc cgcggtcagt ggggtggtgt
     5401 cgatcagttc ctcgcgcagg gcgccgagga tgccgttgcg ggcggcgagc acgcggacct
     5461 tcctggcccg cgcggactgg atgaccgccg aggcggtggc gttgatgacg gcggtgacgc
     5521 cgccgctttg tgcgtagagc agggtgccag cgggcatggg gggatccttg gggcgtgtgc
     5581 aggaggtcgc caggtgcctg gattcgggcc gggatacggt aagctggccc ggttgcgggt
     5641 ccaggcgcag tctagcgccg ccccgcgtgg cgattctgga gcgattcgat gcgattggtt
     5701 cttttgggcg cgcccggctc gggcaagggc acccaggcgg cacgattgaa ggaacacctg
     5761 caggtgccgc acatctccac cggcgacctg ttgcgcgcgg aagtggcggc gggcagcccg
     5821 ctgggcctgc aggccaagga agtgatggcg cgcggcgacc tggtcagcga cgacatcctg
     5881 ctgggcatgc tgcgcgaccg tttttcccgc gacgacacca gggccggctt catcctcgac
     5941 ggctacccgc gcaacctcgc ccaggccgcg gcgctcgaca agctgctcgc ggagctcggc
     6001 caggccttcg atgccgcggt ccagctcacc gtcgacaacg agcagatcat cgagcgcctg
     6061 gccggccggg cgaaggcaga gggccgcgcc gacgacaccc cggagtcggt gcgccaccgc
     6121 ctgaacgtct acgaccagca gaccgcgccg gtgatcgact tctaccgcca gcacggccag
     6181 ctcaccgtgg tcgatggcgt gggctcgctg gacgaggtgt tcacccgcat cctcgaagcg
     6241 atccagccgg ggcacgaggt cggttgacgg cgtcgcagcg aagcccggcg gtcggggggc
     6301 gtttcaattc gctccccgat actcgctcgg ttgaaacgcc ccccgaccac cgatcttcgc
     6361 ccggaatttc tcggagcttg tcttgaaact ccacatcctc ggcatcgccg gtaccttcat
     6421 gggtggcgtc gccgcgctgg cgcgcgaact cggccatgaa gttgaaggca gcgaccagaa
     6481 catctatccg ccgatgtcca cccagctcga acgcctgggc atcgcgctga agcccggcta
     6541 cgaggccgcc aacatctccg ccgattgcga cgagatcgtg gtcggcaatg cgctgtcgcg
     6601 cggcaacgaa gcggtcgaac acgtgctcga tgccggcctc aggtacacct ccggcgcaca
     6661 gtggctgagc gagcggttgt tgcccggccg cgacgtgctg gcggtggccg gaacccacgg
     6721 caagaccacc acgaccacga tcctgtccta ccttctggaa cgcgcgggcc gcgcgccggg
     6781 cttcctgatc ggcggcgtcg ccgaggactt cggcgtgtcc gcgcggatcg gcggtggccg
     6841 cgaattcgtg gtcgaggccg acgaatacga caccgccttc ttcgacaagc gcagcaagtt
     6901 cgtccactac cgcccgctgg tcgcgatcct caacaacctc gagtacgacc acgccgacat
     6961 cttccccgac gtggccgcga tccagcgcca gttccaccac ctggtgcgga ccgtgccgcg
     7021 gcgcggacgg ctgctcgtca acggccacga ccggcacctg cgcgaagtgc tggcgatggg
     7081 ctgctggacg ccggtggagc ggttcggttt cgatccgtcg ttcgaatgga gcgcgcgcaa
     7141 gctgcgcgac gacggcagtg cgttcgtggt cctgcacgct ggccaggagc tcggcaccgt
     7201 cgaatggccg atgctcggcg accacaacgt gctcaacgga ctggccgcgc tcgcggcctg
     7261 cagcgcggtc ggggtcgatg tggcgtcggt catcccgtcg ctcgctgggt tcagcagcgt
     7321 gaagcggcgg atggaagtga tcggcgaaca cgatggcatc accgtctacg acgacttcgc
     7381 ccaccatccc actgcgatcc agaccacgct tgccggcctg cgcgccaagg tcggcgcggc
     7441 aaggatcgtg gtggcgatgg agccgcgcag caattcgatg cgcctgggcg cgcatgcgca
     7501 ggcattggcg ccgtcgctgg acatcgccga cgtggtggtg ttcctggcgc gcccggaatt
     7561 gccgtgggat gcggaccagg tgatcgcggc gatccgcggc gacgcccgcg ccgtggccga
     7621 cgccgatgcg ctggtcgcgc gcctgcgcga aatcgcgcgg gatggcgacc acgtggtgtt
     7681 catgtccaac ggcggtttcg acggcgcccc gcggcgcttc ctcgccgcac tgcgcgatgc
     7741 ctgaggcggg cgcgccgctg ccattgttgc cgctgcaggc ggtgctggtg ccgggcgccg
     7801 ggctggacct gcggatcttc gaacgccgtt acctggacat ggtccgcgac tgcggccgca
     7861 gcggcagcgg cttcggtgtc tgcctgatcc tcgaaggcag cgagaccggc gctgcggcca
     7921 cgccggcggc gttcgggacc gaggcgctga tcgaggattt cggcagcacg ccggatgggt
     7981 tgctgtcgct gcgcgtacgc gggcggcggc gcttccatgt gctgcgcacg cgcgtgcgcg
     8041 acaacgggct gctggtggcc gatgtcgaat ggcgcgagga cgccgccggt tcgccgcggc
     8101 tgcgcccgga gcatgcgctg ctggcggaac tgctgcggcg gatcctgttg caggtcgcgg
     8161 gcgaacacgc cgagccggcg ccactgctgt tcgagagcgc ggcatgggtg ggttggcggc
     8221 tggcggagct gctgccgctg gagatgcggc aacggcaggc gctgctgcag gatgacgacc
     8281 cgcatgcgcg cctgcagcgg ttgctggagg cgatcgccgc ggagggctag cgcttcagcg
     8341 cgtgtcgacg cgcacctgca cccgcggatc ggccacggcc gctctggcgg caagcgcctg
     8401 caggtccgct ggcagcgtgc cggcctgcgg gaccgggtgc cacagggtga gcagtcgcag
     8461 ccgcgtgtgc gcgtgcatgc gttcgtaacg accgacccac agcgcggtgc cgtcgtccag
     8521 ttgcgccggt gccggccaga tccgcaggac ctcgatccag cgcgggtcgt tgccggtccg
     8581 gcgcagcagc aggcgttccg gatgcgcatc cagcgccagc ggcagcaccg gcttgcgcgc
     8641 cagcgatcga tcgtcgtcca gcaggcccag caccgagatc cagtcggcct ggggttgcac
     8701 ccgccaaccc tgcgcctgca ggcgctgctg cagcgggcgc aggtcgccgg cggcctgcag
     8761 gtcgaagtgg cgggggtcgg ggatcccgtg cgcctgccat tgcaccagcg caagccgttg
     8821 ctgcggcgcg ggcggcgtga accgggccag ggtggggtcg gcgctgcgcg gggcgtacca
     8881 accaagggcc agcgcgaaac cgccatagaa catccatgcc agcgggcgca tccagaacga
     8941 gcgcgtgctg tgccgtcggt aggcgatgcc gatcagcaac acccagacca cgcccagcag
     9001 caccccggcg atcacgtcgc tcagccagtg cgcgcccagg taaagccgcg cgaacgcgac
     9061 cagcacggtg gcgacgccgg ccatcaggta cggccagacc cgctgccggc cggggaattc
     9121 gcgcgcgatc aacaccgcga agaagccgaa cacgaccgtg gtcatggtca ccgcgatcga
     9181 cggaaagccg aagccggccg ccgcggtcgg cggccgcggg gtatccacca gcgcatccag
     9241 gccaaccgtc agcaacaggc ctaccgcgat cgccgccagc cagtgcgccg cggccatcca
     9301 gcgccgccgc caacccagcc acagcagcgc ggcaaccgat gcggtgccga gcaccggcgc
     9361 gctgccgatc gcggcgaggt ccgccatcag ccggtcggcg agcggattgc gcagggcgaa
     9421 catcgtcgcc tgcagttcgc ggtcgaacag cagcggctcg ccgcgcacca gcagccaggt
     9481 ggtgaacccg gcccacagcc aggccaccgt gaacaggcag gtcgccagca ccaccagcga
     9541 ggccgattcc ggccgccgcg gatcgatcag cgccgccgca tagcggccca gccgcggatg
     9601 cgcgcgggtc cagcgcagcg cgcgcgccaa caggttgtcc gcgtgcgcgg cgaaccagcg
     9661 ccacgtgtac agcaccagcg cccacccgag tgccagcgcc gcggccaggc ccagcagcac
     9721 cagcaccagc ttgtcggcga ccgcggccac cgcgtcgtag gacgcgccga acgtccagcc
     9781 cggggccagg aacaccgccg cccacgcaaa cgcggcgacc gcgctggcag gcgcgtagcg
     9841 cttcagcggc atgtgcagca tcccggccac cgccggcacg aacggccgca ccgcgccgac
     9901 gaagcgcgcg gtcacgatgc ccttggcgcc gtgccggcgg aacatctgtt cgccgcggtc
     9961 gagcagtccc ggatagcgcc ggaacagcca cagcccgcgc atcgccgggc cccagcgata
    10021 accgatccag tagctcaggc cgtcgccggc gaaggcaccg cccgccgcgc acagcagcgc
    10081 atacgggcca tcgaggtggc ccagcccgat cagggtgccg accgcgaaca gcagcggcag
    10141 cgcaggcacc acgatcccga ccaccgccag cgcgtcgcag aaggcgatca ggaacaccag
    10201 cccgcccgcg gcatgcgggt gggcgctgat ccaggcgatg gtggcgtcga accaggcgct
    10261 ctgcatccgg cgattatagt tggcggccat ttcccgggcg ggcacgtaca cttccgggac
    10321 gcagcaaggg acgcatcgca tggccacgtt gtccggcaag accctgttca tcaccggcgc
    10381 ctcgcgcggg attggcctgg cgatcgcgct gcgcgccgcg cgcgacggcg ccaacatcgc
    10441 gatcgccgcc aagtccgcgg tggcgaatcc gaagttgccc ggcaccatcc acagcgcggc
    10501 ggcgcaggtc gaggccgccg gcggccgcgc gctggcgctc aaatgcgaca tccgcgagga
    10561 ggacgaggtg cgcgccgcgg tcgcggccac ggttgatgcg ttcggcggca tcgacatcct
    10621 ggtcaacaac gccagcgcga tctggctgcg cggcgccctg gacaccccga tgaagcgctt
    10681 cgacctgatg cagcaggtca acagccgcgg cagcttcctg tgcgcgcagg cctgcctgcc
    10741 gcatctgttg caggcgccga acccgcacat cctgaccctg tgcccgccgc catcgctcga
    10801 tcccaaatgg tgggggccgc acgccggcta caccctggcc aagatgggca tgagcttcgt
    10861 gacgctgggc ctggccgccg agttcggccc gcagggggtg gcggtgaatg cgctgtggcc
    10921 gcgcacggtg atcgccaccg acgcgatcaa catgatcccc ggcgtcgacg cgcgcaactg
    10981 ccgcaccccg caggtcgtgg ccgatgccgc gcacgccgtg ctggtccgcg ccgcgcgcgg
    11041 cttccacggc aagttcctga tcgacgaaga cgtcctgcgc gaagccggat gcaccgattt
    11101 cgaccggtac gcggtcgaac ccgggcagcg gctgttgccc gacctgttcc tggagtaagc
    11161 gatgcgctac ctgcacacca tggtccgcgt gcgcgacctc gaggcgaacc tgcgcttctt
    11221 ccgcgacggc cttggcctgc gcgagacccg gcgcatggag aacccgcagg ggcgttacac
    11281 gctggtgttc ctcgccgcgg acgaaacacc cgacgcggaa atcgaactca cgtacaactg
    11341 gcccgacgcc ggcggcgcca tcgaggacta cggcagcgcc cgcaacttcg gccacctggc
    11401 ctttcgcgtc gacgacatct acgcgacctg cgcgcacctg caggcaatgg gctacacgat
    11461 caaccgcccg ccccgcgacg gccacatggc cttcgtgcgc tcgcccgacc tggtctcggt
    11521 ggagctgttg caggacggcc acctgccgcc gcgggagcca tgggcttcga tgcccaacac
    11581 cggcgcgtgg tgagtccgat ggatcgcgcg gacctgctcg ccctcgggcg cgagcgttac
    11641 ctgccggtgt accgcccgcg cgaactgatc ctggagcgtg gacagggcgc gcggctgtgg
    11701 gattccgacg gccgcgacta cgtggacttc ggcgccggca tcgcggtctg cagcctgggc
    11761 cattgcgatc cggaactggt cgccgcgctg caggaacagg ccggcaagct ctggcacacc
    11821 agcaacgtgt tctacagcga gccgccgctg cggctggccg atgaactggt ggtcgcctcg
    11881 cgcttcgccg agcgggtgtt cctgtgcaac tccggcaccg aggcaaacga agccgcgatc
    11941 aagctggtgc gcaagtgggc atcggcgcag ggccgcgcgc cggaccgccg cgtcatcctc
    12001 agcttccgcg gcagtttcca cgggcgcacg ctggcggcgg tgaccgcgac tgcgcagccg
    12061 aagtaccagg agggctacga gccgctgccg ggcggcttcc gctacaccgg cttcaacgat
    12121 ctggaagcgg tggagcaggc gatggccgcg ggcgacgtcg ccgcgatcct ggtcgaaccc
    12181 gtgcagggcg agggcggggt gatgccggca gcgcccggct tcctcgccgg gctgcgcgcg
    12241 ctgtgcgatg cgcatggcgc actgctggtg ctcgatgaaa tccagtgcgg catgggccgc
    12301 accggcagcc tgttcgcgca ttggcaggac gaggcaaccc cggacatcgt gaccctggcc
    12361 aaggcgctcg gcggtggctt cccgatcggc gcgatgctgg ccggcccgca ggttgcacag
    12421 gccatgcagt tcggcgccca cggcaccacc ttcggcggca atccgctggc ggcggcggtc
    12481 gcgcgtgtcg cgctgcgcag gctggcatcg gacgggatcg ccgccaatgt gcgccggcag
    12541 gaacgcgcga tccgcgacgg gctggacgcg atcaaccgcg acctcgacct gttcgccgaa
    12601 gtccgcggcc gcggactgat gctgggtgcc gtgctcaagc ccgaacatgc aggccaggcc
    12661 agcgcgatcc tcgatgccgc cgccgatcac ggcctgctgc tgttgcaggc gggccctgac
    12721 gtgctgcgct tcgtgccggc gctgaacatc ggcgacgacg aagtcgccga tgggctggcg
    12781 cgcctgcgcg cggcactgga gg
//
LOCUS       DAORUK010000054        12778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_4887_length_12778_cov_18.593571, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000054 DAORUK010000000
VERSION     DAORUK010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 12778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12778
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4887_length_12778_cov_18.593571"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..778)
                     /locus_tag="PK818_05275"
                     /pseudo
     CDS             complement(1..778)
                     /locus_tag="PK818_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
     gene            complement(988..1905)
                     /locus_tag="PK818_05280"
     CDS             complement(988..1905)
                     /locus_tag="PK818_05280"
                     /inference="COORDINATES: protein motif:HMM:NF015810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HQY82180.1"
                     /translation="MNATDTEAAGMNPEAVANDLTQLSSREQLSALMDGALPADQTRF
                     LLRRLQHDAPLAECWQRWRLAGEVMRGLAPAQRLPADFALRVGAALHAGAATTAPPQA
                     GRGQASGWLRWGGGAAALAASLAVVALMARQPLPEAPAVAQPVPLASAPAMTPPAPPA
                     ADARTVVDAGSSTEAMAAATVVAAAAPRLARSRSPGQPRTAAPASRAPVRRETAQAQV
                     AVVDGMLGTGIPQPDIVTRPWPRSVLPQYASGGLAVGFGEHARGAVGYNPFQAQSGIG
                     DLPPVADGNAGGDDREAVPAPSPAAEPQP"
     gene            complement(1902..2522)
                     /gene="rpoE"
                     /locus_tag="PK818_05285"
     CDS             complement(1902..2522)
                     /gene="rpoE"
                     /locus_tag="PK818_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007962205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HQY82181.1"
                     /translation="MAEDDVSQALDQDLVARVQRGDSAAFDLLVRKYQHRVGALISRY
                     IHDWAEVQDVAQDTFIRAYRAIGGFRGDAQFSTWLHRIAVNTAKNHLVANRRKPPGDD
                     IELEDAEQFESGLRLRDNDTPERELMRQQLEQTVLRAVEGLPPELRDAITFREVEGMS
                     YEEIAERMDCPVGTVRSRIFRAREAIDARMKPLLDHDDHLPKRSHA"
     gene            2683..4764
                     /locus_tag="PK818_05290"
     CDS             2683..4764
                     /locus_tag="PK818_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HQY82182.1"
                     /translation="MAATHTAPGFDGLRFSHWQVERRDDGVLVLSFDRAESSVNTFSQ
                     DVLLELGALLERIAMEPPKGLLVRSAKAAFAAGADLKSLQALDARGQVDDFIQLGQQV
                     FQRIAELPCPTVAAIHGFCMGGGTEIALACRYRIAASDARIGLPEIKLGIFPGWGGSA
                     RLPRLVGAPAAFDMMLTGRTLSASAARGIGLVDKVVEPAHLQDAALELLKRRLQRPFK
                     QRATGWASNTWLARKILSPMLVKQVARKAPKAHYPAPYALISAWERSGGAGIRGRIAA
                     EKRAVVKLAATPTARNLIRVFFLMERLKAVGGKDSGIRKVHVVGAGVMGGDIAAWSAY
                     KGFEVTLQDREQRFIDGALARAQELFAKRVKDDAKRPAVAARLQGDLAGDGVADADLV
                     IEAIIENPDAKRALYAAVEPRLEADALLTTNTSSIPLDELRGHITRPAQFAGLHYFNP
                     VALMPLVEIIHHDQMAAETQQRLAAFCKALDKLPVPVAGTPGFLVNRVLFPYMLEAMT
                     AYAEGIPGPAIDKVAVKFGMPMGPIELIDTVGLDVAAGVGGELAPFLGLPIPAALQAP
                     PEAGKRGKKDGQGVYAWENGKPKKPELAKDYKAPEDLEDRLLLPLLNEAVACLHDGVV
                     ADAELLDAGVIFGTGFAPFRGGPIQHIRDTGAEVLLARLKALQAKHGDRFAPRAGWDS
                     PLLRADAVAIA"
     gene            complement(4821..5177)
                     /locus_tag="PK818_05295"
     CDS             complement(4821..5177)
                     /locus_tag="PK818_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQY82183.1"
                     /translation="MSSAGGARQGILSLAVKDKAALYNAYMPYIRNGGIFVPTPRRYF
                     IGDEVFLLLILPDSSERLPVAGKVCWVTPTGAQGNRPAGIGVQFADTAEGDAVKGKIE
                     TVLAGTLNAEQPTQTM"
     gene            complement(5191..6195)
                     /locus_tag="PK818_05300"
     CDS             complement(5191..6195)
                     /locus_tag="PK818_05300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HQY82184.1"
                     /translation="MATLPALAPWQRRALAQAAAALAEGHFGHATLVVGPARIGKRAL
                     AEHLAKRVLCLAPSGDGDACGRCRSCTLFASRSQYDPPETRPDGAPSHPWGHSAHPDL
                     RFVGYEINQKTGKPRAEIVIEQVRALSEAMTLTPRLGGAQVVIVDPADAINWSAFNAL
                     LKTLEEPQPGRYLWLLSANPARLPATIRSRCQRLELRLPPADEALQWLATRGHRGELA
                     REALDAARGHPGLADAWASGDGLALRRAVAQDLQVLQRGQAAPAEVAQRWVADGRADE
                     RLRHLAELALQEAAGLTDPARTRTLAARFDAANRTRDLLRSTVRADLAVTETLLGWCA
                     "
     gene            complement(6204..6863)
                     /gene="tmk"
                     /locus_tag="PK818_05305"
     CDS             complement(6204..6863)
                     /gene="tmk"
                     /locus_tag="PK818_05305"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019336901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HQY82185.1"
                     /translation="MSAGLQRQPRLLTIEGGEGAGKSTVLRALRDALEAEGFEVVCTR
                     EPGGTPLAERIRALLLDPSHEPAAPETELLLMFAARAQHVREVVLPALQRGAWVVSDR
                     FTDSSYAYQGAARGLDPAFIAELERRVVGIEPGLTLLLDLGVAHGRERTRGRDLLGGS
                     SPDRIERERDEFFESVRAGFLQRAAQHPQRIRVLDASPDANQVAASALAALATYREAR
                     A"
     gene            complement(6860..7933)
                     /gene="mltG"
                     /locus_tag="PK818_05310"
     CDS             complement(6860..7933)
                     /gene="mltG"
                     /locus_tag="PK818_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HQY82186.1"
                     /translation="MSRRRTRRGKAGGFFRRTLLLLLVAALAMGGWYWQRYRSFADAP
                     LAGVEAGTSLVVARGDSLASVLRKLQDAGIAPGDRLQWQLLARELGAAGKLQVGEYAL
                     QPEASPRSLLLAMRDGKVVRRMFTIVEGWNIRDLRKALAKVESLQQDTAALDDAALMK
                     ALGHPGQHPEGRFLPETYAWVRGDSDLDLLGRAHAAMARELDAAWAARAADVPLESKE
                     QALVLASIVEKETGIAEERPAIAGVFARRLEIGMRLQTDPTVIYGMGAAYTGNIRRSD
                     LTTDTPYNTYTRDGLPPTPIAMPGAAALRAATRPADGDALYFVAVGDGSGRHLFSASL
                     GAHNAAVRAYVQRYRAQQRSQPK"
     gene            complement(7930..8751)
                     /gene="pabC"
                     /locus_tag="PK818_05315"
     CDS             complement(7930..8751)
                     /gene="pabC"
                     /locus_tag="PK818_05315"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR03461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HQY82187.1"
                     /translation="MSTRCYSGEREVAAIDPRNRGMAYGDGLFETMRVHDGGLPLWPR
                     HLARLREGAQRLGIGLPDVPFIEARITEMIGATDAGVLKLLLTRGEGGRGYAPPADAQ
                     PVWLLALHPLPAVPGHGLRLRWCDMRLASQPALAGIKHCNRLEQVLARAEAESAGCDE
                     GLMRDMAGNPICATSANLLVLRDGRWSTPRVDACGVAGVLRGWLLEQGLVESVVLAQG
                     EVESAAALALCNAVRGILPVASLGARAWAPHPALAELQERVAMAYPMFSEAGAAA"
     gene            complement(8748..10100)
                     /locus_tag="PK818_05320"
     CDS             complement(8748..10100)
                     /locus_tag="PK818_05320"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014506896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HQY82188.1"
                     /translation="MISRTLPAGLDLLDLHRLDPARYRVLLESSATGPHGRWDMLLLH
                     AGESFALGRDGVVRDQHGARMEGRFLDVLDRQWQAHRQPRGRDASLPFRGGWALLLGY
                     ELAAQVEPVLALPAPAGGLPVACALRCPAAVLRDRGSGECIAVAEAGEEAWLKRIVAD
                     VARAAALPALPEWQPPAVVDEDPPQRYVDGVARVLDYLAAGDVFQANLSRGWQVSFDA
                     ALDPAALFQRLRGNNPAPFAGVFRGIGWSVVSASPERLVSVHGDVVETRPIAGTRPRF
                     AGDDDAERIRELVGHPKERAEHVMLIDLERNDLGRVCEAGSVVVDELMTVESYAHVHH
                     IVSNVRGTLKGDATPGGVIAAVFPGGTITGCPKVRCMQVIAELEGVGRGAYTGAMGWL
                     DHAGDMDLNILIRSAELEEDTLRFRTGAGIVIDSDPRRELDETRAKARGMLRALGVAG
                     "
     gene            complement(10157..11395)
                     /gene="fabF"
                     /locus_tag="PK818_05325"
     CDS             complement(10157..11395)
                     /gene="fabF"
                     /locus_tag="PK818_05325"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HQY82189.1"
                     /translation="MSKRRVVVTGMGIVSPLGNDLASNWDAICNGRSGIGPVTGFDAA
                     TFATRIAGEIRDFDVTRWVSPKEAKKMDHFIHYGVAASLMAMADAGLEVTEANAERIG
                     ALIGAGIGGIWGIEETAIKLHEGGVRKISPFYIPSTIINMLPGQLSLMTGIKGPNFSA
                     VSACATANHSIGMAMRMIQYGDADVMFAGGAERGSSPTSMGGFCAMKAMSTRNDDPAR
                     ASRPWDAGRDGFVLGDGAGILVLEEYEHAKARGARIYCELAGFGASSDAFHMTAPSEN
                     GEGPARCMAMAFRDAGVNQEEVGYLNAHGTSTPLGDVAETLAIKRALGDHAYRTMVSS
                     TKSMTGHLLGAAGGVEAIYTIKALQTGIIPPTINLETPGEGCDLDYVPNTAREAQVQV
                     AVSNGFGFGGTNGTLVFRRI"
     gene            complement(11489..11728)
                     /gene="acpP"
                     /locus_tag="PK818_05330"
     CDS             complement(11489..11728)
                     /gene="acpP"
                     /locus_tag="PK818_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQY82190.1"
                     /translation="MSSIEERVKKIVVEQLGVKEEDVTTSASFVDDLGADSLDTVELV
                     MALEEEFECEIPDEEAEKISTVQAAIDYIKAHVKA"
     gene            complement(11815..12561)
                     /gene="fabG"
                     /locus_tag="PK818_05335"
     CDS             complement(11815..12561)
                     /gene="fabG"
                     /locus_tag="PK818_05335"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HQY82191.1"
                     /translation="MSGQVLKGEVALVTGASRGIGAAIADELAALGATVIGTATSDAG
                     ALAIGERLAAVGGHGRRLNVNEAGAIEALVDAVASEFGGISILVNNAGITRDNLLMRM
                     KDEDWQAILDTNLTSVYRASKAVMRGMMKARRGRIVNIASVIGVTGNAGQANYAAAKA
                     GIIAFSKSLAKEIGSRGITVNVVAPGFIATDMTADLPEQARQAMLGQIALGRLGEPAD
                     IARAVAFLAGPGASYITGETLHVNGGMYMA"
     gene            complement(12558..>12778)
                     /locus_tag="PK818_05340"
     CDS             complement(12558..>12778)
                     /locus_tag="PK818_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malonyl CoA-acyl carrier protein transacylase"
                     /protein_id="HQY82192.1"
                     /translation="AIRDALVRQLYLPVQWTGCVQALAACGVTRVAECGPGKVLTGLV
                     KRIDKSLDARALGTPADFTAAIEEWKGA"
BASE COUNT         1830 a         4583 c         4460 g         1905 t
ORIGIN      
        1 tcaccttgcc ggtcgccttg agctgctgca ccgcgttcat cgccacgtcg atcggaatgg
       61 cgaagctcac gcccatgtag ccaccggaat tgctgaagat ctgcgagttg atgccgacca
      121 cctggccgcg ggtgtccagc agcgggccgc cggagttgcc gcggttgatc gccacgtcgg
      181 tctggatgaa cggcacgtag cgctggttcg catagggatt ggcgcgacca accgcgctga
      241 cgatgccggc tgtcacggag tgatcgaggc cgaacggcga accgatcgcg accacccact
      301 ggcccgactt cagcgccttg gaatcaccga tgcgcagggt gggcaggccc gtcgcggcga
      361 tcttcagcag ggccacgtcc gactgctcgt cgctgccgac caccctggcc acgaactcac
      421 ggcggtcgga cagcttgatc cgcacggtgt ccgcgccctc gaccacgtgg tggttggtca
      481 gcacgtagcc gtcggcggaa atgatgaagc cggtgccctg ggagaccccg cgcggacgtg
      541 cgccgggcat gccgggggcg cccggcatcg gcatgccatc ggggccgaag aagcgccgga
      601 agaattccgg gatctcttca tcgccgggct gctgcggctg cgcccgctgg gcgtcggcgg
      661 cacgcggctt tccgcccact tcggcctgga tgctgaccac cgccgggccg acctggtcga
      721 ccaggcgggt gaagtccggc agcccgacca ccagctccgg cgccggcgcg gttgcctgcg
      781 cctgttgcgc gtgcgcggac gaataccagc cggcgccact gccgatggcc agggccagcg
      841 cggcggacag cagcagcatc gaaggacgcc ggggacgagg gacgcagacg ggggtgggaa
      901 cggacatcgt ggacgaatcc tcggtcgcgg tggtggtggt tcgggcggtg gatggaactg
      961 gaatcaggaa gctgtgccgg gggccgctca aggctgcggt tcggcggcgg gcgacggcgc
     1021 ggggaccgcc tcgcggtcgt cgccgccggc attgccgtct gccaccggtg gcaagtcgcc
     1081 gatgccggac tgggcctgga acgggttgta gccgaccgcg ccgcgcgcgt gttcgccgaa
     1141 acccacggcg agccctccgc tggcgtattg cggcagcacc gaacgcggcc acggccgggt
     1201 cacgatgtcg ggctgcggga tgccggtgcc gagcatgcca tcgaccactg caacctgcgc
     1261 ctgcgccgtc tccctgcgca ccggcgcacg ggacgcgggc gccgcggttc gcggctgtcc
     1321 agggctgcgg ctgcgcgcca gccgcggggc ggctgcggcc accacggtgg cagccgccat
     1381 cgcctcggtg gagctgccgg cgtcgaccac cgtgcgtgcg tccgctgccg gcggcgcggg
     1441 cggcgtcatt gcaggtgctg acgccagcgg caccggttgc gccaccgccg gtgcctccgg
     1501 cagcggctgc cgcgccatca acgcgaccac cgccagcgac gccgccagcg ccgccgcacc
     1561 accgccccaa cgcagccatc cagatgcctg cccgcggcct gcctgcggtg gcgcggtggt
     1621 cgccgccccg gcgtgcagcg ccgcgcccac ccgcaacgcg aaatcggcgg gcaggcgttg
     1681 cgccggggcg agcccgcgca tcacctcgcc ggcaaggcgc cagcgctgcc agcattcggc
     1741 gagtggcgcg tcgtgctgca ggcggcgcag caggaagcgg gtctggtccg ccggcaacgc
     1801 accatccatc aacgcggaca gctgttcgcg gctgctgagc tgggtcaggt cgttggcgac
     1861 ggcttcggga ttcatgcctg cggcctcggt atcggtggcg ttcatgcgtg cgagcgtttc
     1921 ggcaggtggt cgtcgtggtc gagcagcggc ttcatgcgcg catcgatcgc ctcgcgggcg
     1981 cggaagatgc gcgaacgcac ggtcccgacc gggcagtcca tgcgctcggc gatctcctcg
     2041 tagctcatgc cttccacctc gcggaaggtg atcgcgtcgc gcagctcggg cggcagtcct
     2101 tcgaccgcgc gcagcaccgt ttgttcaagc tgctggcgca tcagctcgcg ttccggggtg
     2161 tcgttgtcgc gcaggcgcag gccggattcg aactgctcgg cgtcctccag ctcgatgtcg
     2221 tcgcccggcg gcttgcgcct gttggcgacc aggtggttct tcgcggtgtt gaccgcgatc
     2281 cggtgcagcc aggtggagaa ctgggcatcg ccgcggaagc cgccgatcgc ccggtaggcg
     2341 cggatgaagg tgtcctgcgc cacgtcctgc acttccgccc agtcgtggat gtagcgcgaa
     2401 atcagcgcac cgacgcgatg ctggtacttg cgcaccagca agtcgaaggc agcgctgtcc
     2461 ccgcgctgca cgcgggcgac gagatcctgg tcaagcgctt gcgacacgtc atcctcggcc
     2521 attgcgccgg tgcctcgcga gtgggcccgt gcgtccggac cctgtgacat tggtggccgt
     2581 ggaaagttcc cgcgggggcg gcggcgggcg ttttttgagt ggtattcaaa tcgacccggg
     2641 ataggatacg gccctaccca tactgcttcg gatggtacgc gcatggccgc cacccacacc
     2701 gcgcccggct tcgatggact gcgtttctcc cactggcagg tcgagcgccg cgacgacggc
     2761 gtgctggtgc tgtcgttcga ccgcgccgaa tcgagcgtga acaccttcag ccaggacgtg
     2821 ctgctggagc tgggtgcctt gctggaacgg atcgcgatgg agccgccgaa ggggttgctg
     2881 gtgcgctcgg cgaaggccgc gttcgccgcc ggcgccgacc tcaagtcgct gcaggcgctc
     2941 gacgcacgcg gccaggtcga cgatttcatc cagctcggcc aacaggtgtt ccagcgcatc
     3001 gccgaactgc cctgccccac ggtggccgcg atccacggct tctgcatggg cggcggcacc
     3061 gagatcgcgc tggcctgccg ctaccggatc gccgccagcg acgcccgcat cggcctgccc
     3121 gagatcaagc tcggcatctt ccccggctgg ggcggcagcg cacgcttgcc gcggctggtc
     3181 ggcgcgccgg ccgcgttcga catgatgctg accggccgca cgctgtcggc gtccgccgca
     3241 cgcgggatcg gcctggtcga caaggtggtg gagccggcgc acctgcagga tgccgcgctg
     3301 gagctgctca agcgccggct gcagcgcccg ttcaagcagc gcgccacggg ctgggcgagc
     3361 aacacctggc tggcgcgcaa gatcctgtcg ccgatgctgg tcaagcaggt cgcgcgcaag
     3421 gcaccgaagg cgcactaccc ggcgccgtat gcgctgatct cggcgtggga gcgcagcggc
     3481 ggcgccggca tccgcggccg gatcgcggcg gagaagcggg cggtggtgaa actggccgcc
     3541 accccgaccg cgcgcaacct gatccgggtg ttcttcctga tggagcggct gaaggcggtg
     3601 ggcggcaagg actccggcat ccgcaaggtg cacgtggtcg gcgccggcgt gatgggcggc
     3661 gatatcgccg cgtggagcgc ctacaagggc ttcgaggtca ccctgcagga ccgcgagcag
     3721 cgcttcatcg acggcgccct cgcccgcgcc caggaactgt tcgccaagcg ggtcaaggat
     3781 gatgcgaagc gcccggccgt ggccgcacgt ttgcagggtg accttgccgg cgacggcgtc
     3841 gccgatgccg acctggtcat cgaggcgatc atcgagaacc cggacgccaa gcgcgcgctg
     3901 tatgccgcgg tggagccgcg cctggaggcg gatgcattgc tcaccaccaa cacctcgtcg
     3961 atcccgctcg acgaactgcg cggccacatc acgcgcccgg cgcagttcgc cggcctgcac
     4021 tacttcaacc cggtggcgct gatgccgttg gtggagatca tccaccacga ccagatggcg
     4081 gcggaaaccc agcagcgcct ggccgcattc tgcaaggcgc tggacaagct gccggtgccg
     4141 gttgccggca cccccggctt cctggtcaac cgggtactgt tcccgtacat gctggaagcg
     4201 atgaccgcgt atgccgaggg catccccggc cctgcgatcg acaaggtcgc ggtgaagttc
     4261 gggatgccga tggggccgat cgaactgatc gacaccgtcg gcctcgacgt cgctgccggc
     4321 gttggcggcg aactggcccc gttcctgggc ctgccgatcc cggccgcgct gcaggcgccg
     4381 ccggaagccg gcaagcgcgg caagaaggac ggccagggcg tgtacgcctg ggagaacggc
     4441 aagccgaaga agccggaact ggcgaaggac tacaaggcac ccgaagacct cgaggaccgc
     4501 ctgctgctgc cgctgctgaa cgaagcggtg gcctgcctgc acgatggcgt ggtcgccgat
     4561 gccgaactgc tggatgccgg ggtgatcttc ggtaccggct tcgcgccgtt ccgcggcggc
     4621 ccgatccagc acatccgcga caccggcgcc gaggtgctgc tggcgcggct caaggcgctg
     4681 caggcaaagc acggcgaccg cttcgcaccg cgtgcgggct gggattcccc gctgctgcgc
     4741 gcggacgccg tggcgatcgc ctgatgcgaa gaagcccggc gatgccgggc ttcggtcgat
     4801 tcgacggcgg cggcatccgc ctacatggtc tgggtgggct gctcggcatt gagggtgccg
     4861 gccagcacgg tctcgatctt gcctttcacc gcatcgccct cggcggtgtc ggcgaactgc
     4921 acgccgatcc ccgccgggcg gttgccctgt gcaccggtcg gggtgaccca gcagaccttg
     4981 ccggcgaccg gcaggcgctc ggatgaatcc ggcaggatca gcagcaggaa cacctcgtcg
     5041 ccgatgaagt agcggcgcgg cgtcggcacg aagatgccgc cgttgcggat gtacggcatg
     5101 taggcgttgt acagcgcggc cttgtccttc accgccagtg acaggatgcc ctgccgcgcg
     5161 ccgcctgcac tgctcatgcg tacgtcccct tcatgcgcac cacccgagta atgtttcagt
     5221 gaccgccagg tcggcgcgca ccgtgctgcg cagcaggtcg cgggtccggt tggcggcatc
     5281 gaaacgcgcg gccagcgtgc gggttcgcgc cggatcggtc aagcccgcgg cctcctgcag
     5341 ggccagctcc gccaggtggc gcaggcgctc gtcggcgcgg ccgtcggcca cccagcgttg
     5401 cgccacctcc gccggcgcgg cctgcccgcg ctgcagcacc tgcaggtcct gggcgaccgc
     5461 acggcgcagc gccaggccgt cgccggaggc ccaggcatcg gccagccccg gatgcccgcg
     5521 tgccgcatcc agcgcttcgc gcgcaagctc gccgcggtgc ccgcgcgtgg ccagccattg
     5581 cagggcctcg tccgccggcg gcagccgcag ctccaggcgc tggcaacggc tgcggatggt
     5641 cgccggcagc cgcgccgggt tggcggacag cagccacagg taacgacccg gctgcggttc
     5701 ctccagcgtc ttcagcagcg cgttgaaggc gctccagttg atcgcatcgg cgggatccac
     5761 gatgaccacc tgcgcgccgc ccaggcgcgg ggtcagggtc atcgcctcgg acaacgcccg
     5821 gacctgctcg atcacgatct ccgcgcgcgg cttgccggtc ttctggttga tctcgtagcc
     5881 gacgaaccgc aggtccgggt gcgcgctgtg gccccacgga tgcgacggcg ccccatccgg
     5941 ccgggtctcc ggggggtcgt attgcgaacg cgacgcgaac agggtgcagc tcctgcacct
     6001 gccgcaggcg tcgccatcgc cgcttggcgc caggcacaac acgcgcttgg ccaggtgttc
     6061 ggccagcgcg cgcttgccga tccgcgccgg gccgacgacc agggtggcat gcccgaaatg
     6121 cccctccgcc aacgccgcgg ccgcctgcgc caaggcgcgc cgctgccacg gggcaagtgc
     6181 cggcaacgtg gcgatggcag cgctcacgcg cgtgcctcgc ggtacgtagc cagcgccgcc
     6241 agcgccgagg cggcgacctg gttcgcatcg ggactggcgt caagcacccg gatccgctgc
     6301 ggatgctgcg ccgcgcgctg caggaagccg gcgcgcacgg attcgaagaa ttcgtcgcgc
     6361 tcgcgctcga tccggtccgg cgaggaaccg ccgagcaggt cgcgtccgcg cgtgcgctcg
     6421 cggccgtggg cgacgccaag gtcgagcagc agggtcaggc ccggttcgat cccgaccacc
     6481 cgccgctcca gctcggcgat gaacgccgga tccaggccgc gcgccgcgcc ctggtaggcg
     6541 tagctggaat cggtgaagcg gtcgctgacc acccaggcgc cccgctgcag cgccggcagc
     6601 accacttcgc ggacatgctg cgcgcgcgcg gcgaacatca gcagcaactc ggtttccggc
     6661 gcggccggct cgtgcgacgg atccagcagc agcgcgcgga tgcgctcggc cagcggcgtg
     6721 ccgccgggtt cgcgggtgca gacgacctcg aagccctctg cctccagcgc gtcgcgcagc
     6781 gcgcgcagca ccgtgctctt gcccgcgccc tcgccgccct cgatggtgag caggcgtggc
     6841 tgccgttgca ggcccgcgct cacttcggtt gcgaccgttg ctgcgcgcgg tagcgctgca
     6901 cgtacgcgcg gaccgcggcg ttgtgcgcac ccagcgaggc cgagaacaga tgccgcccgc
     6961 tgccgtcgcc gaccgccacg aaatacagcg catcgccgtc cgcgggccgg gtggcggccc
     7021 gcagcgcggc ggcaccgggc atcgcgatcg gcgtgggtgg caagccgtcg cgggtatagg
     7081 tgttgtaggg cgtgtcggtg gtgaggtcgc tgcggcggat gttgccggtg taggcggcac
     7141 ccatgccgta gatcacggtc gggtcggtct gcaggcgcat gccgatctcc agcctgcgcg
     7201 cgaacacgcc ggcgatcgcc gggcgttcct cggcgattcc ggtttccttt tccacgatcg
     7261 aggccagcac cagcgcctgc tccttcgatt ccagcggaac gtcggccgcg cgcgcggccc
     7321 acgccgcgtc cagttccctg gccatcgccg catgcgcgcg tccaagcagg tccaggtcgg
     7381 aatcgccgcg cacccaggca taggtttccg gcaggaaacg gccttcggga tgctggccgg
     7441 ggtggcccag cgccttcatc agcgccgcgt cgtccagtgc cgcggtgtcc tgctgcaggg
     7501 actccacctt ggccagcgcc ttgcgcaggt cgcggatgtt ccagccctcg acgatggtga
     7561 acatgcgccg caccaccttg ccgtcgcgca tcgccagcag cagcgaacgc ggcgacgcct
     7621 ccggctgcaa cgcgtactcg cccacctgca gcttgccggc cgcgcccagt tcgcgcgcca
     7681 gcagctgcca ttgcaggcgg tcgccgggtg cgatgcctgc gtcctgcagc ttgcgcagca
     7741 cgctggcgag cgagtcgccg cgcgcgacca ccaggctggt gccggcctcg accccggcca
     7801 gcggcgcatc ggcgaagctg cggtaacgct gccaatacca gccgcccatg gccagcgcgg
     7861 ccaccagcag caacagcagc gtgcggcgga agaaaccgcc cgccttgccg cgacgcgtcc
     7921 ggcgccggct cacgccgccg ccccggcttc ggaaaacatc ggatacgcca tcgccactcg
     7981 ttcctgcaat tcggccagcg ccgggtgcgg tgcccacgcg cgcgcgccca gcgacgcgac
     8041 cggcaggata ccgcgcaccg cattgcaaag cgccaatgcc gcggccgact ccacctcacc
     8101 ctgcgcgagc acgacggact cgaccagccc ctgctccagc agccatccgc gcagcacgcc
     8161 ggccacgccg catgcgtcca cgcgcggggt cgaccagcga ccgtcccgca acaccagcag
     8221 gttggccgac gtcgcgcaga tggggttgcc cgccatgtcg cgcatcaggc cttcgtcgca
     8281 gccggcggac tcggcctcgg cgcgcgccag cacctgctcc agccggttgc agtgcttgat
     8341 cccggccagc gccggctgcg acgcgagccg catgtcgcac cagcgcaggc gcagtccatg
     8401 ccccggcact gccggcagcg gatgcagcgc cagcaaccac accggctgcg cgtcggcggg
     8461 tggcgcgtag ccgcgcccgc cttcgccgcg ggtgagcagc agcttgagca cgccagcgtc
     8521 ggtcgcgccg atcatttcgg tgatgcgcgc ctcgatgaag gggacatcgg gcaggccgat
     8581 cccgagccgc tgcgcacctt cgcgcaggcg cgccaggtgg cgcggccaca acggcagccc
     8641 gccgtcatgg acacgcatgg tttcgaacag gccgtcgccg taggccatgc cgcgattgcg
     8701 cggatcgatg gcggcgacct cgcgctcgcc cgagtagcag cgcgtgctca acccgccacc
     8761 cccagcgcgc gcaacatgcc gcgcgccttg gcccgcgtct cgtccagttc gcgccgtgga
     8821 tcggaatcga tcacgatgcc ggcgccggtg cggaagcgaa gtgtgtcttc ctccagctcg
     8881 gcactgcgga tcaggatgtt gaggtccatg tcgccagcat ggtccagcca gcccatcgcg
     8941 ccggtgtagg cgccgcgacc gacgccctcc agttcggcga tgacctgcat gcagcgcact
     9001 ttcgggcagc cggtgatggt gccgccgggg aacaccgcgg cgatcacccc gcccggcgtt
     9061 gcatccccct tcagggtgcc gcgcacattg ctgacgatgt ggtgcacgtg ggcgtagctt
     9121 tccaccgtca tcagttcgtc gacgaccacg ctgccggcct cgcagacgcg cccgaggtcg
     9181 ttgcgctcga ggtcgatcag catcacgtgc tcggcgcgtt ccttcggatg cccgaccagc
     9241 tcgcggatgc gttcggcgtc atcgtcgcca gcgaaacgcg ggcgggtgcc ggcgatcggc
     9301 cgggtttcca ccacatcgcc atgcaccgac accaggcgct ccggcgaggc actgaccacg
     9361 ctccagccaa tgccgcggaa cacccccgcg aacggcgccg ggttgttgcc gcgcaggcgc
     9421 tggaacaggg ccgccggatc cagcgcggca tcgaacgaaa cctgccagcc gcgcgacagg
     9481 ttggcctgga acacgtcgcc ggccgcaagg taatccagca cgcgcgccac gccatcgaca
     9541 tagcgctgcg gcgggtcttc atcgaccacc gcgggcggct gccactccgg caacgccggc
     9601 aacgctgcgg cacgcgcgac atccgccacg atccgtttga gccaggcttc ctcgccagct
     9661 tcggcgacag cgatgcattc gcccgagccg cggtcgcgca gtaccgccgc cgggcagcgc
     9721 agcgcgcagg ccaccggcag accgcccgcg ggcgccggca gcgccagcac cggttcgacc
     9781 tgcgccgcca gttcatagcc aagcagcaag gcccagccac cgcggaacgg caggctggca
     9841 tcgcggccgc ggggctggcg gtgtgcctgc cactgccggt ccagcacgtc caggaaacgg
     9901 ccttccatgc gcgcgccgtg ctggtcgcgg accacgccat cgcgcccaag cgcgaacgat
     9961 tcgccggcgt gcagcagcag catgtcccag cgcccgtgcg gcccggtcgc gctggattcc
    10021 agcagcaccc ggtagcgcgc tggatcgagg cgatgcaggt cgagcaggtc gaggccggcg
    10081 ggaagggttc ggctgatcat cggcgaagtg caccccaccg caaccctccc ctgcttcgca
    10141 agggagggag tcggcatcag atgcgacgga acaccagggt gccgttggtg ccaccgaagc
    10201 cgaagccgtt cgacaccgcc acctgcacct gcgcctcgcg cgcggtgttc ggcacgtagt
    10261 cgaggtcgca gccttcgccc ggcgtctcca ggttgatggt cggcgggatg atcccggtct
    10321 gcagcgcctt gatcgtgtag atcgcctcca ccccgccggc ggcaccgagc aggtgcccgg
    10381 tcatcgactt ggtcgagctc accatcgtcc ggtaggcgtg gtcgcccagc gcgcgcttga
    10441 tcgccagcgt ttcggcgaca tcaccgagcg gggtgctggt gccgtgcgcg ttgaggtagc
    10501 cgacctcctc ctggttgacg ccggcatcgc ggaacgccat cgccatgcag cgcgcgggac
    10561 cttcgccatt ctcgctgggc gcggtcatgt ggaaggcatc cgaggacgcg ccgaagccgg
    10621 ccagctcgca gtagatgcgc gcaccgcgcg ccttggcgtg ctcgtactcc tccagcacca
    10681 ggatgccggc gccgtcgccc agcacgaagc cgtcgcgccc ggcgtcccac ggccgcgagg
    10741 cgcgggccgg gtcgtcgttg cgggtgctca tcgccttcat cgcgcagaag ccgcccatcg
    10801 aggtcggcga gctgccgcgc tcggcgccgc cggcgaacat cacgtcggcg tcgccgtact
    10861 ggatcatccg catcgccatg ccgatcgaat ggttggcggt ggcgcaggcg gagaccgcgg
    10921 agaaattcgg gcccttgatc ccggtcatca gcgagagctg gcccgggagc atgttgatga
    10981 tggtgctggg gatgtagaac gggctgatct tgcggacccc gccctcgtgc agcttgatcg
    11041 cggtttcctc gatcccccag atgccgccga tgcccgcgcc gatcagcgcg ccgatgcgct
    11101 cggcgttggc ctcggtcact tccagcccgg cgtcggccat cgccatcagc gaggcggcca
    11161 ccccgtaatg gatgaagtgg tccatcttct tcgcttcctt cgggctcacc cagcgggtga
    11221 cgtcgaagtc gcggatctcg ccggcgatgc gggtggcgaa ggtggcggca tcgaagccgg
    11281 tgaccggacc gatgccggaa cggccgttgc agatggcgtc ccagttgctg gccaggtcgt
    11341 tgcccagcgg cgagacgatg cccatcccgg ttacgacgac gcgacgcttc gacatggcgt
    11401 ttccccgatt ggtcctgttc gatgatgcgg tggtgcaaac gcgcggggcc gcatcagcgg
    11461 ccccggcgtc gaaacccacg aaggcaggtc aagccttgac gtgggccttg atgtaatcga
    11521 tcgcggcctg cacggtgctg atcttctcgg cttcttcgtc cgggatctcg cactcgaact
    11581 cttcctcgag cgccatcacc agttccaccg tatccagcga atccgcgccg aggtcgtcca
    11641 cgaacgacgc gctggtggtg acgtcctctt ccttgacgcc cagttgttcg acgacgatct
    11701 tcttgacgcg ctcttcgatg ctgctcatgt ctggatgctc ctccccgggg tggggtcagt
    11761 cggtggttag tttacgtgaa agccggccgg cgcaagcacc ggcaggcggt ggaatcaggc
    11821 catgtacatg ccgccgttga cgtgcaatgt ctcgccggtg atgtagctgg cgccgggccc
    11881 cgcgaggaag gccaccgcgc gcgcgatgtc ggccggctcg cccagccggc ccagcgcgat
    11941 ctgcccgagc atggcctgcc gggcctgctc cggcaggtcg gcggtcatgt cggtggcgat
    12001 gaagccgggc gcgactacgt tgacggtgat cccgcggctg ccgatttcct tcgccagcga
    12061 cttgctgaag gcgatgatcc cggccttggc cgcggcgtag ttggcctggc cggcgttgcc
    12121 ggtgacgccg atcaccgagg cgatgttgac gatgcggccg cggcgcgcct tcatcatccc
    12181 gcgcatcacc gccttggagg cgcgatacac cgaggtcagg ttggtgtcga ggatcgcctg
    12241 ccagtcctcg tccttcatcc gcatcagcag gttgtcgcgg gtgatgccgg cgttgttgac
    12301 caggatcgag atgccgccga actcgcttgc aaccgcgtcg accagcgcct cgatcgcgcc
    12361 ggcctcgttg acgttcaggc ggcgcccgtg gccgccgacc gcggccaggc gctcgccgat
    12421 cgccagcgcg cccgcgtccg aggtcgcggt accgatcacg gtcgcaccca gcgcggccag
    12481 ttcgtcggcg atcgccgcgc cgatcccgcg cgacgcaccg gtgaccagcg cgacttcgcc
    12541 cttcagcact tgcccgctca tgcgcccttc cactcttcga tggccgcggt gaagtccgcc
    12601 ggcgtgccca acgcacgcgc gtcgagcgat ttgtcgatgc gcttgaccag gccggtcagc
    12661 accttgcccg ggccgcactc ggcaacgcgg gtgaccccgc atgcggcgag cgcctgcacg
    12721 caaccggtcc attgcaccgg caggtagagc tggcgcacca gcgcgtcgcg gatcgcat
//
LOCUS       DAORUK010000055        12344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_5159_length_12344_cov_17.902596, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000055 DAORUK010000000
VERSION     DAORUK010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 12344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12344
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5159_length_12344_cov_17.902596"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PK818_05345"
     CDS             <1..181
                     /locus_tag="PK818_05345"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HQY82193.1"
                     /translation="GFSRAYLRHLDRCNEMLAPMLGTLHNLRYYQRLMAGMRAAIETG
                     TFAAFRDAFYRARRA"
     gene            259..603
                     /gene="yajC"
                     /locus_tag="PK818_05350"
     CDS             259..603
                     /gene="yajC"
                     /locus_tag="PK818_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HQY82194.1"
                     /translation="MNPLDFLIPVAHAQAAGAAPAAPGMMSTLLFPVILIAIMYFLMI
                     RPQMKRQKEHRAMLDKLSRGDEVITNGGIAGTVSEIGESFVTVEIAAGVQVRLQKGAI
                     ANVLPKGTLKAA"
     gene            658..2526
                     /gene="secD"
                     /locus_tag="PK818_05355"
     CDS             658..2526
                     /gene="secD"
                     /locus_tag="PK818_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HQY82195.1"
                     /translation="MLEYARWKYVVILLVLALSVLYAVPNVFPSEPAVQVTANRGASV
                     DAALQQRVEAALKKSAVPFASSEIGKDGNLLVRIASDDAQSRASQAIRDELGGSYVVA
                     LNQASTVPKWLQWVGAKPMFLGLDLRGGVHFLMQVDREAALAKRFEATAEDMRVQFRE
                     NRILFTSIEPAPGNRVVARLRPGQDLDKARQLLARDMPTYRIEALGDTITVAIPQAEL
                     DRMLTEAVQQNIGTINNRINELGVAEPIIQRQGADRVVVELPGVQDTAQAKRILGATA
                     TLEYRGVVEGNAVEARDNGLVPPEARLYNRKETGTDGKPMPILLNKRVIASGEQLQNA
                     TSFFDSQSGTPAVSVRLNSIGGQRMFDYSSANVGKPMAVVYIERIPEVKLVDGKEVRS
                     TRIMEQVISVATIQGTLGRDFQTTGLESMKESADLALLLRAGALAAPMDFVDERTIGP
                     SLGKENIERGLKAVAFSFLFALGFFLVYYRMFGLITSLALLINLLMVVALMSLLGFTL
                     SLPGLAGIALTVGMSVDANVLINERIREELRAGMPPQSAIATGYEKATGTIADANLTA
                     MLAGIALFAFGSGPLKGFAVALILGILTSMYTAVSVSRGIATLIYGGKRKLKSIAI"
     gene            2539..3495
                     /gene="secF"
                     /locus_tag="PK818_05360"
     CDS             2539..3495
                     /gene="secF"
                     /locus_tag="PK818_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012438079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HQY82196.1"
                     /translation="MQPFNVFPHDSTFDFMRLRLASLVAAALLMVVALGAIPLKGFNF
                     ALDFTGGVGVELRYPQAPDVDGVRARLAASGYEDAQVQAFGSGNDLLVRLQADGKQEG
                     GADQASSIGDAVASAASLDGNPATKMSSAVISAQVGKELAWNGFYALLFVVLGFLAYI
                     SARFEWKFAVAAIITTLHDVLICAGWFALSGHEFDLTVLAGILSVMGYSINDTIVVFD
                     RVRENFKSLRVPPREVLNKSINQTLSRTVITSFVALLTVLALYFYGGGSLRGMAEAQM
                     IGIVVGTLSSIFIACPLLLWLGVSKQDLLPKARDEAALARRP"
     gene            complement(3584..4402)
                     /locus_tag="PK818_05365"
     CDS             complement(3584..4402)
                     /locus_tag="PK818_05365"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQY82197.1"
                     /translation="MQKPVVNVMVKAARAAGNVLLRSMHKLDAINVVEKDRMDFASDV
                     DEAAEKAAVKELRRAYPDYAILAEEGGAQGKGHSVFVIDPLDGTSNFLRGFPHWCVSI
                     ALVEGGDVQHGVIFDPLRNELFTASRGSGAVLNDKRIRVGERKDLAGAMLATGFAPRE
                     RERAPAQLECIRELLRDAEDIRRTGSAALDLAYVACGRLDGYFEAGVKPWDIAAGVLL
                     VREAGGRICDFRGAGTGSLDAKGIAGRTLVAGNLKLIDSLQKGIVQSGYAAACR"
     gene            4500..5282
                     /locus_tag="PK818_05370"
     CDS             4500..5282
                     /locus_tag="PK818_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQY82198.1"
                     /translation="METLLESTDRDLRIVLVGTQHPGNIGSAARAMKTMGLSRLVLVA
                     PEKAPDRDTQAMAAGADDLVAAAPVFASLAEAVADCRWVLGCTARSRRIQLEPLHPRD
                     AARRALAATASGPVALVFGRERTGLENDELQLCHAAIHIPSDPAFSSLNLAAAVQVLS
                     YELRCAMLGDLPALQDSAQRTAPPAAGAASHAELEGLFGQLAEALEQIDFHKGRAPDS
                     AMRKLRRLYLRANLDSADVRLLRGVLADTQRMARLAGQGESP"
     gene            5360..6277
                     /locus_tag="PK818_05375"
     CDS             5360..6277
                     /locus_tag="PK818_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HQY82199.1"
                     /translation="MAGLLCILLALLPSARAQVAAPVEDDASVLVLGRVSDDPKSHYD
                     QLKPLLDYVVPRMASVGIRSGRILMARDLQQMGSYLRRGRVDWVTETSGNAVLLERRA
                     NARSLLITERDGASRYHSVFFVRRDSPVRSLADLAGRSVAFQSPYSTSAYYLPASTLL
                     EAGQKMELLLSPMDKPSPQAVGYLFARTELNITTWVHKRLVDAGVLSNLDWANPQRMP
                     PAFVADFRVVAESQDVPRALVLTRGDLEPKVEARLREVLMEASIDPDAGEALRRFIGT
                     SRFVDVSAEDRQSLDRLGRGLTRVRLEVE"
     gene            6274..8412
                     /locus_tag="PK818_05380"
     CDS             6274..8412
                     /locus_tag="PK818_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017168504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQY82200.1"
                     /translation="MRRPRMGLQSRFFAAILLLLALLVLVMVLVWQRQQSTQHEVSEV
                     TRVAMHGLLSEQLRVDGEAEVRQLADGLANPLYFFDLDAIGALARAALRESDVDYVLV
                     YDPQGRILHDGSGDIAAYGQPMGDALAKEVIAARGPHTRMQGQVLDVSSPIMVADQRL
                     GGVRIGYSLVGMAAAEEQAMQGLRGRIDEVGRRNLAWLLLLCAALGVVGLAAAALVQR
                     LLVQPIKQLGDAAREIEAGNFSLPVPQTNREDELGDLVRTFGRMRESVAKHDRDIRRV
                     AYSDALTGLSNRLAFRESLDQRLRSLQGAGRQLALVFADLDDFKRVNDTLGHEAGDEV
                     LLQIAGRIDRAVKAIAGADAMVARFGGDEFVILLQAGDDEPDGDVRTMVTELAKGLVA
                     DLGEPLILHGRQVFLGSSVGVALFPDDASSASLLMKNGDIAMYQAKVAGKNCYRFYSR
                     TMDQAVERRMRMEHDLRGAWGRGELSLAYQPIFRTQDRVLVGAEALLRWNHPEDGAIS
                     PAVFIEVAEQSGLIEAIGPEVMRAACQEASRWHKPAGAGEPPFVSVNVSPRQLRSTDL
                     VQQIGSALQESALSPERLHVELTETAVISDEANATALLGRLRETGVRVWLDDFGTGYS
                     SLSHLRRVQVDGLKIDRSFITDLLRDPDDLSLTSAIIAMAHSMGIVVVAEGVEKQGQY
                     ELLRERDCDLVQGYWLGHPLSAAEFVKLLG"
     gene            complement(8423..9070)
                     /locus_tag="PK818_05385"
     CDS             complement(8423..9070)
                     /locus_tag="PK818_05385"
                     /inference="COORDINATES: protein motif:HMM:NF017833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HQY82201.1"
                     /translation="MAPPEPRDPARTRKPREAGTRAILDVLLDGDHQQALDFSALLGG
                     LGRRAFGMLLFVAALPAFIPIPIGGALSGPLVVLIAAQLLVGLRRPWLPGFIARRGPK
                     RQSLARFERIVDPWLLRLEHLVRPRLAAVLDHRLAAAFTGLLLLLLGVLLSLPIPLTN
                     YLFGGIILVFALALLERDGALMLGAWGLGLAALATTGALSGGLVAALQPVLGRFL"
     gene            complement(9057..10382)
                     /locus_tag="PK818_05390"
     CDS             complement(9057..10382)
                     /locus_tag="PK818_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQY82202.1"
                     /translation="MAELLVVFALIALNGFFAMSEMAVMTSRKLRLKQLAEDPERPSR
                     GARKALAMAEHPDHLLSTVQVGITLIGICTGVFGGDAIGLQIADWLRGAWPDAHQYAR
                     PLGIGTAVALITASSVIFGELIPKRLALTNPEAIASRVAIPLDLLATATKPVILLLGA
                     INRGVLRLLGIRNDARSAITEEEIHLLVSESHEQGVIDLDERNMMNRVMRLGDRTAES
                     LMTPRTDIAWLDASGSLADNLATMQDTPFSRYPVYRGGDDDVAGVLEVKTLLDELGKQ
                     LPELFRDIRPALFVSESTHALKLVEILREEQQSLALVVDEYGDIQGVVTISDVMDAVL
                     GRLQAGEAHDDEALVVEREDGSLLVDGGLHIDELRELTGERLADTEEHDYHTAAGLVI
                     AHFGRIPHVGEHFMLGSWRIEVVDLDGPRIDKLLLQRMPGARDGGDGAA"
     gene            10570..11196
                     /locus_tag="PK818_05395"
     CDS             10570..11196
                     /locus_tag="PK818_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pit accessory protein"
                     /protein_id="HQY82203.1"
                     /translation="MFSLQTIFGQGKQFYALFNEAADAAYDSTKALHAMLKAAERSPS
                     LEPFKHARQRERVASDKISQALVESFITPFEREDIEALGSALYKIPKQVERFADRYAL
                     AQHRLVNIDFAPRAAMLEQAAGVVVEMVAELPQLRLEKMKALNDRLRVIETEADRLIL
                     ELHREIYSGDLDAGDMFLLKEFFEIMEKAIDRCREAGVVAYEIVLKNS"
     gene            11218..12339
                     /locus_tag="PK818_05400"
     CDS             11218..12339
                     /locus_tag="PK818_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQY82204.1"
                     /translation="MLTLLLLVILSALVFEFINGFHDTANSIATVVATKVLSPGHAVM
                     LAAVMNLIGAFAGTAVAKTISSGLVDTEVVTITSQVLVCALAGGISWNLVTWWMGLPS
                     SSSHALIGGLCGAALAAAHNDWNALIWSKLGDGGWTANKGILWKVVVPMVSSPIAGFT
                     LGVLILALLYALVAGLRKAGGPLARLSRPRWVNAFFRKAQLASAAYMGFAHGTNDAQK
                     TMGIIALAIFAAQGAGTLDNLPAWAQFLHPDGGETDIDKWIVVSCALVMAAGTAAGGW
                     RIIKTLGHKMVKLQPIDGFAAETASATILVTAAHFGMPVSTTHSISTAIMGVGFGKNP
                     RALKLTVIERIVWAWVLTIPAAGGFAYGLLRLVEALGWA"
BASE COUNT         1890 a         4215 c         4323 g         1916 t
ORIGIN      
        1 cggcttcagc agggcctacc tgcgccacct ggaccgttgc aacgagatgc tggcgccgat
       61 gctgggcacc ctgcacaacc tgcgctacta ccagcgcctg atggcgggga tgcgcgcggc
      121 catcgaaacg gggaccttcg cagccttccg cgacgcgttc taccgggcac ggcgggcttg
      181 aaacggccct tgaatgggcg tgttccggcc tacatggcat aattcccggc tgttttgaca
      241 gtcatgcacg gattcccgat gaacccgctc gatttcctga ttcccgttgc ccatgcccag
      301 gccgccggcg ccgcacccgc cgcacctggc atgatgtcca ccctgctgtt cccggtcatc
      361 ctgatcgcga tcatgtactt cctgatgatc cgcccgcaga tgaagcggca gaaggagcac
      421 cgcgcgatgc tggacaagct gtcccgcggc gacgaggtga tcaccaacgg cggcatcgcc
      481 ggcaccgtca gcgagatcgg cgagagcttc gtcaccgtcg agatcgccgc cggcgtgcag
      541 gtccgcctgc agaagggcgc gatcgccaat gtcctgccca agggcacgct gaaagccgcc
      601 tgatccttcc cgattgcttg aagcgccggc cccgcgggcc gtgcgcgggg tgctgcaatg
      661 ctcgaatacg cccgttggaa atacgtcgtc atcctgctcg tgctggcatt gagcgtcctg
      721 tacgcggtgc cgaacgtgtt cccgtcggaa ccggcggtgc aggtcaccgc caaccgcggc
      781 gccagcgtcg acgccgccct gcagcagcgg gtcgaggccg cgctgaagaa gtcggcggtg
      841 ccgttcgcct ccagcgagat cggcaaggac ggcaacctgc tggtgcgcat cgccagcgac
      901 gatgcccaga gccgcgcctc ccaggccatc cgcgacgagc tcggcggcag ctacgtggtc
      961 gcgctgaacc aggcgtccac ggtgccgaag tggctgcagt gggtcggcgc caagccgatg
     1021 ttcctcggcc ttgacctgcg tggcggcgtg catttcctga tgcaggtcga ccgcgaggcc
     1081 gcgctggcca agcgcttcga ggcgaccgcc gaggacatgc gggtgcagtt ccgcgaaaac
     1141 cggatcctgt tcacctcgat cgagccggcg cccggcaacc gggtggtcgc gcggttgcgc
     1201 cccggccagg acctggacaa ggcccgccag ttgctggcgc gcgacatgcc gacctaccgg
     1261 atcgaggcgc tgggcgacac gatcacggtc gcgatcccgc aggctgagct tgaccggatg
     1321 ctgaccgagg cggtgcagca gaacatcggc accatcaaca accgcatcaa cgagctcggc
     1381 gtggccgagc cgatcatcca gcgccagggc gcggaccggg tggtggtcga gctgccgggc
     1441 gtgcaggaca ccgcgcaggc caagcgcatc ctcggcgcca ccgcgaccct ggaataccgc
     1501 ggcgtggtcg aaggcaatgc ggtggaggcg cgcgacaacg gcctggtgcc gccggaagca
     1561 cggctctaca accgcaagga aaccggcacc gacggcaagc cgatgccgat cctgctcaac
     1621 aagcgggtga tcgcctccgg cgagcagctg cagaatgcga cctcgttctt cgactcgcag
     1681 tccggcaccc cggcggtcag cgtgcgcctc aacagcatcg gcggccagcg catgttcgac
     1741 tactccagcg ccaacgtcgg caagccgatg gcggtggtgt acatcgagcg catccccgag
     1801 gtgaagctgg tcgacggcaa ggaagtgcgc agcacccgga tcatggagca ggtgatctcg
     1861 gtcgccacca tccagggcac gctgggccgc gacttccaga ccaccggcct cgagagcatg
     1921 aaggaatccg ccgacctcgc cctgctgctg cgcgccggtg cgctggcggc gccgatggac
     1981 ttcgtcgacg aacgcaccat cggcccgagc ctgggcaagg agaacatcga acgcggcctc
     2041 aaggcggtgg cgttctcctt cctcttcgcg ctgggcttct tcctggtcta ttaccggatg
     2101 ttcggcctga tcacctcgct cgcgctgctg atcaacctgc tgatggtggt ggcgctgatg
     2161 tcgctgctgg gcttcaccct cagcctgccg gggcttgccg gcatcgcgct gaccgtgggc
     2221 atgtcggtgg acgccaacgt gctgatcaac gagcgcatcc gcgaggaatt gcgcgccggg
     2281 atgccgccgc aatcggcgat cgccaccggg tacgagaagg cgaccggcac catcgccgac
     2341 gccaacctca ccgcgatgct ggccggcatc gccctgttcg cgttcggcag cggcccgctg
     2401 aagggcttcg cggtggcgtt gatcctcggc atcctgacct cgatgtacac cgcggtatcg
     2461 gtatcgcgcg gcatcgccac cctgatctat ggcggcaagc gcaagctgaa gtcgatcgcg
     2521 atctgacgga gaccgcacat gcaacccttc aacgtgttcc cgcacgacag caccttcgac
     2581 ttcatgcgcc tgcggctggc gtcgctggtg gcggccgccc tgctgatggt ggtcgcgctt
     2641 ggcgcgatcc cgctcaaggg cttcaacttc gcgctggact tcaccggcgg cgtcggcgtc
     2701 gagttgcgct acccgcaggc gccggatgtc gatggcgtgc gcgcccggct ggcggcgtcc
     2761 ggctacgagg atgcgcaggt gcaggcgttc ggcagcggca acgacctgct ggtgcggctg
     2821 caggccgacg gcaagcagga gggtggcgcc gaccaggcca gcagcatcgg cgacgcggtc
     2881 gccagcgccg cttcgcttga cggcaatccc gcgaccaaga tgagcagcgc ggtgatcagc
     2941 gcccaggtcg gcaaggagct ggcctggaac ggcttctacg cgctgctgtt cgtggtgctg
     3001 ggcttccttg cctacatctc ggcgcgcttc gagtggaagt tcgcggtggc ggcgatcatc
     3061 accaccctgc acgacgtgct gatctgcgcc ggctggttcg cgctgagcgg gcacgaattc
     3121 gacctgaccg tgctggccgg catcctgtcg gtgatgggct attcgatcaa cgacaccatc
     3181 gtggtgttcg accgcgtgcg cgagaacttc aagtcgctgc gggtgccgcc gcgcgaggtc
     3241 ctgaacaagt cgatcaacca gaccctgtcg cgtaccgtta tcacctcgtt cgtggcgctg
     3301 ctgaccgtgc tggccctgta cttctatggc ggcggttcgc tgcgcgggat ggccgaggca
     3361 cagatgatcg gcatcgtggt cggtaccctg tcctcgatct tcatcgcctg cccgctgctg
     3421 ctgtggctgg gcgtgagcaa gcaggacctg ctgccgaagg cacgcgacga ggcggcgctg
     3481 gcgcggcgcc cctgaccggg cgggtccatg gcccacaaga aacccgcgca gtgcgcgggt
     3541 ttcttgtggg tgaagcgacc ccaggccaag ccgggagtcg cgactacctg caggcggcgg
     3601 cgtagccgga ctgcacgatc cccttctgca ggctgtcgat cagcttgagg ttgccggcca
     3661 ccagcgtgcg cccggcgatc cccttcgcat ccagcgaacc ggtgccggcg ccgcggaaat
     3721 cgcagatgcg gccgccggcc tcgcgcacca gcagcacccc ggcggcgatg tcccacggct
     3781 tcacgccggc ctcgaagtag ccgtccaggc ggccgcaggc cacgtaggcg aggtcgagcg
     3841 cggcggagcc ggtgcgccgg atgtcctcgg cgtcgcgcag cagttcgcgg atgcattcca
     3901 gctgcgccgg cgcgcgctcg cgctcgcgcg gggcgaagcc ggtggccagc atcgcgccgg
     3961 ccaggtcctt gcgctcgccg acgcggatgc gcttgtcgtt gagcaccgcg ccgctgccgc
     4021 ggctggcggt gaacagttcg ttgcgcagcg ggtcgaagat gaccccgtgc tgcacgtcgc
     4081 cgccttcgac cagcgcgatc gacacgcacc agtgcgggaa gccgcgcagg aagttgctgg
     4141 tgccgtccag cgggtcgatc acgaacaccg aatggccctt gccctgcgcg ccgccttcct
     4201 cggcgaggat cgcgtaatcc gggtaggcgc ggcgcagttc cttgaccgcc gccttctccg
     4261 cggcttcgtc gacgtcgctg gcgaagtcca tgcggtcctt ctcgaccacg ttgatcgcat
     4321 cgagcttgtg catgctgcgc aggagcacgt tgccggccgc gcgggccgcc ttgaccatga
     4381 cattgaccac gggtttctgc atggcggagc gcctcgaagg ggtgaggagg ggggaacggc
     4441 ggcggcggca aagaacgacc ccggcgcggg ggccggggcg cgcagtttac cattccggga
     4501 tggagaccct gctcgaatcc accgaccgcg acctgcgcat cgtcctggtc ggtacccagc
     4561 acccaggcaa catcggttcc gcggcccgcg cgatgaagac catgggcctg tcgcggctgg
     4621 tgctggtggc gccggaaaag gccccggacc gcgataccca ggcgatggcg gcgggcgccg
     4681 acgacctggt cgcggcggca ccggtgttcg ccagcctggc cgaagcggtt gccgactgcc
     4741 gctgggtact gggatgtacc gcgcgcagca ggcgcatcca gcttgagccg ctgcacccgc
     4801 gcgatgccgc ccggcgcgcg cttgccgcca ccgccagcgg gccggtggcg ctggtgttcg
     4861 ggcgcgagcg caccgggctg gagaacgacg aactgcagct gtgccacgcc gcgatccaca
     4921 ttccctcgga tccggcgttc agttcgctga acctcgccgc cgcggtgcag gtgctcagct
     4981 acgagttgcg ttgcgcgatg ctgggcgatc tgccggcact gcaggacagc gcgcagcgca
     5041 cggcgccgcc ggctgcgggc gcggccagcc atgccgagct cgagggtctg ttcggccagc
     5101 ttgccgaggc gctcgagcag atcgatttcc acaagggacg cgcgcccgac tcggcgatgc
     5161 gcaagctgcg ccggctgtac cttcgggcca acctggacag cgccgacgtg cgcctgttgc
     5221 gcggcgtgct cgccgatacc cagcgcatgg cgcggctggc ggggcagggc gaatcgccat
     5281 gaaaatcggt taggctccgc gcaggggaac caggaaccgg atcgtcttgc actcgacgct
     5341 gcgaaacccc aggccggtcg tggctggcct gctatgcatc ctgctggcgc tgctgccgtc
     5401 ggcgcgcgcg caggtggctg cgccggttga agacgatgct tcggtgctgg tgctggggcg
     5461 ggtcagcgac gacccgaagt cgcactacga ccagctcaag ccgctgctcg actacgtggt
     5521 gccgcggatg gccagcgtcg gcatccgcag tggccgcatc ctgatggcgc gcgacctgca
     5581 gcagatgggc agctacctgc gccgtggccg ggtcgactgg gtgaccgaga cctccggcaa
     5641 cgcggtcctg ctggagcgcc gcgccaacgc acgctcgctg ctgatcaccg aacgcgatgg
     5701 cgccagccgc taccacagcg tgttcttcgt gcgccgcgac agcccggtgc gcagcctggc
     5761 cgacctggcc ggacgcagcg tggcgttcca gagcccgtat tcgaccagcg cgtactacct
     5821 gccggccagc accctgctcg aggccggcca gaagatggag ctgctgctct cgccgatgga
     5881 caagccttcg ccgcaggcgg tgggttacct gttcgcgcgc accgaactca acatcaccac
     5941 ctgggtgcac aagcggctgg tcgatgccgg cgtgctcagc aacctcgact gggccaaccc
     6001 gcagcggatg ccgccggcgt tcgtcgccga tttccgggtc gtggccgaaa gccaggacgt
     6061 gccgcgcgcg ctggtgctga cccgcggcga cctcgagccg aaggtcgagg cgcgcctgcg
     6121 cgaggtgctg atggaggcgt cgatcgaccc ggatgccggc gaggcgctgc gccgcttcat
     6181 cggcacctcc cgcttcgtcg acgtctccgc cgaggaccgg cagtcgctgg accggcttgg
     6241 ccgtggcctc acccgcgtgc gcctggaggt cgaatgaggc ggccgcggat gggcctgcaa
     6301 tcgcggttct tcgccgcgat cctgctcctg ctggcgctgc tggtgctggt gatggtgctg
     6361 gtctggcagc gccagcagtc gacccagcac gaagtcagcg aagtcacccg ggtggccatg
     6421 cacggcctgc tgtcggagca gctgcgggtg gatggcgagg ccgaagtgcg gcagcttgcc
     6481 gatggcctgg ccaatccgct gtatttcttc gacctcgatg ccatcggcgc gctggcgcgt
     6541 gccgcgctgc gcgaatccga cgtcgattac gtgctggtgt acgacccgca gggccgcatc
     6601 ctgcacgacg gctccggcga catcgccgcc tacgggcagc cgatgggcga tgcgctggcg
     6661 aaggaagtga tcgccgcgcg cggcccgcac acccggatgc aggggcaggt gctcgacgtg
     6721 tccagcccga tcatggtcgc ggaccagcgc ctcggcggcg tgcgcatcgg ctattcgctg
     6781 gtcggcatgg ccgctgcgga ggaacaggcg atgcagggcc tgcgcgggcg catcgacgaa
     6841 gtcggccgcc gcaacctcgc ctggctgctg ctgctgtgcg cggcgctcgg cgtggtcggc
     6901 ctggccgcgg cggcactggt gcagcggctg ctggtgcagc cgatcaagca gctcggcgat
     6961 gccgcgcgcg agatcgaggc cggcaatttc tcgctgccgg tgccgcagac caatcgcgag
     7021 gacgaactcg gcgacctcgt ccgcaccttc ggccgcatgc gcgaaagcgt ggccaagcac
     7081 gaccgcgaca tccggcgggt ggcctacagc gatgccctga ccggcctgtc caaccggctg
     7141 gcgttccgcg agtcgctgga ccagcgcctg cgttcgctgc agggcgccgg ccgccagctg
     7201 gcgctggtgt tcgccgacct cgacgacttc aagcgggtca acgacaccct cggccacgaa
     7261 gccggcgacg aggtgctgtt gcagatcgcc ggccgcatcg accgcgcggt gaaggcgatc
     7321 gccggcgccg acgcgatggt cgcgcgcttc ggcggcgacg aattcgtgat cctgctgcag
     7381 gccggcgacg acgagcccga tggcgacgtg cggacgatgg tcaccgaact ggccaagggg
     7441 ctggtcgccg accttggcga accgctgatc ctgcatggcc ggcaggtgtt cctcggctcc
     7501 tcggtcgggg tggcgctgtt cccggacgac gcctccagcg catcgctgct gatgaagaac
     7561 ggcgacatcg cgatgtacca ggccaaggtc gccggcaaga actgctaccg cttctacagc
     7621 cgcaccatgg accaggcggt cgaacggcgg atgcgcatgg agcacgacct ccgcggcgcg
     7681 tggggccgcg gcgaactcag cctcgcctac cagccgatct tccgcaccca ggaccgggtg
     7741 ctggtcggcg cggaggcgct gttgcgctgg aaccacccgg aggatggcgc gatctcgccg
     7801 gcggtgttca tcgaggtcgc cgagcagagt ggcctgatcg aggcgatcgg gccggaggtc
     7861 atgcgcgccg cctgccagga agcgtcgcgc tggcacaagc ccgcgggtgc tggcgaaccg
     7921 ccgttcgtct cggtcaatgt ctcgccgcgg cagctgcgca gcaccgacct ggtccagcag
     7981 atcggcagcg cgctgcagga gtcggcactg tcgccggaac gcctgcacgt ggagctcacc
     8041 gaaaccgcgg tgatcagcga cgaggccaac gccaccgcct tgctcggccg cctgcgcgag
     8101 accggcgtgc gggtctggct ggacgacttc ggcaccggct attccagcct cagccacctg
     8161 cgccgggtcc aggtcgacgg cctgaagatc gaccgcagct tcatcaccga cctgctgcgc
     8221 gaccccgacg acctgtccct gaccagcgcg atcatcgcga tggcgcattc gatggggatc
     8281 gtggtggtcg cggagggcgt cgaaaagcag ggccagtacg aactgctgcg tgagcgcgat
     8341 tgcgacctgg tgcagggcta ctggctgggg catccgctca gcgcggcgga gttcgtgaag
     8401 ctgctcggct gacccgcggg cgttagagga acctgccaag cacgggttgc agcgcggcga
     8461 ccagcccgcc ggacagcgcg ccggtggtgg ccagcgcggc cagccccagg ccccaggcgc
     8521 ccagcatcaa ggcgccgtcg cgctcgagca gggccagcgc gaacaccagg atgatcccgc
     8581 cgaacaggta gttggtcagc gggatcggca gcgacagcag cacgcccagc agcagcagga
     8641 gcaggccggt gaacgcggcc gccaggcggt ggtcgaggac cgcggccagc cgcggccgca
     8701 ccaggtgctc cagccgcagc agccacggat cgacgatgcg ctcgaagcgc gccagcgact
     8761 gccgcttggg gccgcgccgg gcgatgaacc ccggcagcca cggccggcgc aggccgacca
     8821 gcaactgggc cgcgatcagc accaccagcg ggccgctgag cgcgccgccg atcgggatcg
     8881 ggatgaacgc cggcagcgcg gccacgaaca gcagcatccc gaacgcgcgc cggccgaggc
     8941 cgccgagcag ggcgctgaaa tcgagtgcct gctggtggtc gccgtcgagc aggacgtcca
     9001 ggatcgcgcg ggtgccggct tcgcggggct tgcgcgtgcg tgccgggtcg cgtggctcag
     9061 gcggcgccat cgccgccatc cctggcgccg ggcatgcgct gcagcagcag cttgtcgatc
     9121 cgcggcccgt ccaggtccac gacttcgatc cgccaggagc cgagcatgaa atgctcgccc
     9181 acgtgcggga tgcggccgaa atgggcgatc accagcccgg cggcggtgtg gtaatcgtgt
     9241 tcctcggtgt cggccaggcg ctcgccggtg agttcgcgca gctcgtcgat gtgcaggccg
     9301 ccgtcgacca gcagcgagcc gtcctcgcgt tcgaccacca gcgcctcgtc gtcgtgcgcc
     9361 tcgccggcct gcaggcgacc gagcaccgca tccatgacgt cgctgatggt gaccacgccc
     9421 tggatgtcgc cgtattcgtc gaccaccagc gccagcgact gctgttcctc gcgcaggatc
     9481 tcgaccagct tcagcgcgtg ggtggattcg gacacgaaca gcgccgggcg gatgtcccgg
     9541 aacagctccg gcagctgctt gcccagctcg tccagcaggg tcttcacctc aagcacgccg
     9601 gcgacgtcgt cgtcgccacc gcggtagacc gggtagcgcg agaacggcgt gtcctgcatg
     9661 gtcgccaggt tgtcggccag gctgccgctg gcgtccagcc aggcgatgtc ggtgcgcggc
     9721 gtcatcaggc tttccgcggt gcggtcgccg aggcgcatga cccggttcat catgttgcgt
     9781 tcgtcgaggt cgatcacgcc ctgctcgtgg ctttcgctca ccagcaggtg gatctcttcc
     9841 tcggtgatcg cgctgcgggc gtcgttgcgg atgccgagca ggcgcagcac gccgcggttg
     9901 atcgcgccca gcagcaggat caccggcttg gtggcggtgg ccagcaggtc cagtggtatc
     9961 gccacccggc tggcgatggc ttccggattg gtcagcgcca gccgcttggg gatcagttcg
    10021 ccgaagatca ccgaactggc ggtgatcagc gccaccgcgg tgccgatgcc cagcgggcgc
    10081 gcgtactggt gcgcatccgg ccaggcgccg cgcaaccagt cggcgatctg caggccgatc
    10141 gcatcgccgc cgaacacgcc ggtgcagatc ccgatcaggg tgatcccgac ctgcacggtg
    10201 gacagcaggt ggtcggggtg ttcggccatc gccagcgcct tgcgcgcgcc gcggctgggg
    10261 cgttccggat cctcggccag ctgcttgagg cgcagcttgc gcgaggtcat caccgccatc
    10321 tccgacatcg cgaagaaccc gttcagcgcg atcagggcga acaccaccag caattcagcc
    10381 acgggcgctc ccgtgccgcg cggcccgcaa gcgggcgcgg aaggtcgacg gcggtggctt
    10441 gggggactgg tccatggagt gcgccggggc gcgccgcgat gatagcaggg cagggcatga
    10501 tgacggccgc gcattaatgt catccagccg tcataatccg caccgacgcc aaacgacggg
    10561 caggccgcca tgttttcgct gcagaccatt ttcggccagg gcaagcagtt ctatgccctg
    10621 ttcaacgaag cggccgacgc cgcctacgac agcaccaagg ccctgcacgc gatgctcaag
    10681 gcggccgagc gctcgccctc gctggagccc ttcaagcacg cgcgccagcg cgagcgggtc
    10741 gcctccgaca agatcagcca ggcgctggtg gaaagcttca tcaccccgtt cgagcgcgag
    10801 gacatcgagg cgctggggtc tgcgctgtac aagatcccga agcaggtgga acgcttcgcc
    10861 gaccgctacg cactcgccca gcaccggctg gtcaacatcg acttcgcccc gcgcgcggcg
    10921 atgctggagc aggccgccgg ggtggtggtg gagatggtcg cggagctgcc gcagctgcgc
    10981 ctggagaaga tgaaggcgct caacgaccgc ctgcgggtga tcgagaccga ggccgaccgg
    11041 ctgatcctgg agctgcaccg cgagatctat tcgggcgacc ttgatgccgg cgacatgttc
    11101 ctgctcaagg aattcttcga gatcatggaa aaggccatcg accgctgccg cgaggcgggg
    11161 gtggtggcgt acgagatcgt gctgaagaat tcctgagccg gcgaccggag acgcacgatg
    11221 ctgaccctgt tgctgctggt gatcctgtcc gcgctcgtat tcgagttcat caacggcttc
    11281 cacgacaccg ccaactccat cgccaccgtg gtcgcgacca aggtgctttc gcccgggcat
    11341 gcggtgatgc tggccgcggt gatgaacctg atcggcgcgt tcgcgggcac cgcggtggcc
    11401 aagaccatct cctccggcct ggtcgatacc gaggtggtca cgatcacctc gcaggtgctg
    11461 gtctgtgcgc tggccggcgg catcagctgg aacctggtga cctggtggat ggggctgccg
    11521 tcgtcctcgt cgcacgcgct gatcggtggc ctgtgcggcg ccgcgctcgc ggctgcccac
    11581 aacgactgga acgccctgat ctggtcgaag ctcggcgatg gcggctggac cgccaacaag
    11641 ggcatcctgt ggaaggtggt ggtgccgatg gtcagttcgc cgatcgccgg cttcaccctc
    11701 ggggtgctga tcctggcgtt gctgtatgcg ctggtggcgg gcttgcgcaa ggccggcggg
    11761 ccgttggcgc ggctgtcgcg gccgcgctgg gtgaatgcgt tcttccgcaa ggcgcagctg
    11821 gcttcagcgg cctacatggg cttcgcccac ggcaccaacg acgcgcagaa gacgatgggc
    11881 atcatcgccc tggcgatctt cgccgcgcag ggcgcgggca ccctggacaa cctgccggca
    11941 tgggcgcagt tcctgcatcc ggatggcggc gagaccgaca tcgacaagtg gatcgtggtg
    12001 tcgtgcgcgc tggtgatggc ggccggcacc gccgccggcg gctggcggat catcaagacc
    12061 ctgggccaca agatggtcaa gctgcagccg atcgacggct tcgccgcgga aaccgcttcg
    12121 gccacgatcc tggtcaccgc cgcccacttc ggcatgccgg tctcgaccac ccacagcatc
    12181 tccaccgcga tcatgggcgt gggtttcggc aagaacccgc gtgcgctgaa gctgaccgtg
    12241 atcgagcgga tcgtatgggc gtgggtgctg accatccccg ccgccggcgg ctttgcctac
    12301 gggctgctgc gcctggtcga ggcactgggc tgggcctgag ggca
//
LOCUS       DAORUK010000056        12053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_5375_length_12053_cov_19.497083, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000056 DAORUK010000000
VERSION     DAORUK010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 12053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12053
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5375_length_12053_cov_19.497083"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..356
                     /locus_tag="PK818_05405"
     CDS             75..356
                     /locus_tag="PK818_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82205.1"
                     /translation="MMDHRGDTANRITDITNGVDSAETWDFGYDALSRLVTAQSPASL
                     GGFGYDAIGNRISRSSNGVQNATLTYPGTSSRLQTYAASGLLLVVRPHA"
     gene            complement(330..593)
                     /locus_tag="PK818_05410"
     CDS             complement(330..593)
                     /locus_tag="PK818_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HQY82206.1"
                     /translation="MSWRVVFTRQAQKDARTLASSGLKEKAKALLSVLAENPFQTPPP
                     YEKLVGDLAGAYSRRINIQHRLVYEVLQVERTVKVLRMWTHYE"
     gene            complement(590..832)
                     /locus_tag="PK818_05415"
     CDS             complement(590..832)
                     /locus_tag="PK818_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014980433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HQY82207.1"
                     /translation="MTTLTASEARANLYRLMDQAAESHEPIVISGKRSNAVLIAEEDW
                     AAIQETLFLLSVPGMRESIKSGMAEPANACATELDW"
     gene            complement(978..1613)
                     /locus_tag="PK818_05420"
     CDS             complement(978..1613)
                     /locus_tag="PK818_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQY82208.1"
                     /translation="MGDLQLRVPQPWQQPIAPHSRKRSYRDGDVLFHEGDPSDGVYIV
                     LSGRLKVYAAGGNGDEVVYTVLGPGELLGEVSLFGGPRTASVRAIGAVECLVLRNSAA
                     QDLMRTRPEFAAHVFAKLASRVRHSIRMTRSLALDGVPERVSALLEACAVHDGQALRV
                     PAELTQQEIANRIGASREMVLKVIGKLVKQGFLHKDARHRMTILKSLGGAR"
     gene            complement(1789..4647)
                     /locus_tag="PK818_05425"
     CDS             complement(1789..4647)
                     /locus_tag="PK818_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQY82209.1"
                     /translation="MSTLFAATSLDGIEAVAAREAVSSFGVRDLRLHRQPAGRDGAPV
                     RKHHKVSTLPIDIRRAARGSQRAWFDLEGAPEPVALSVEWPELGGPDPAAADWLGFLA
                     IVAARVRTALELEDQRLVATRLEKTVRIQTALYRVANLASAGLEMADMLARIHDVVGE
                     LMYARNFYIALHDRERDSLRFIYFADEQDDHGVDPAQEYPAAEIRNSLTLGVIRSGQP
                     ALGPSRALREAFGLEQDNEQYGPGSAHWLGVPMIADGVVRGAIVVQSYDPATRYSADD
                     QSLLQFVAQHILIALVRHQAHVELEHRVEERTRALTDEVRERQHGERLQAALYGIADL
                     ASSELGMADMLRRIHLMVGELIYARNFYIALYDGERDSVRFIYFADAKDVNIYETDRE
                     VPAADLRDSLTLGLIRHGKPVRGPARDVARALGISGIGLGTPALDFLGVPISGDSGAR
                     GAVVVQTYEPGVHYSAEDSTLLGYVAQHIMTALERKKLQAELERRVEDRTRELAKVVR
                     ALREQVKVREQAEQRLTHDALHDALTGLPNRTCFHQGLEQALARLHGDAGHRFAVLFL
                     DLDRFKVINDSVGHLYGDMMLKEAAGRLAGCVRDGDMVARLGGDEFAVLMLDIAGADD
                     ACHTAGRVIDALSGPMQVGGKELFTSASVGITLGEPRYRKAEELLRDADVAMYRAKAH
                     GRQRFELFDERLHQEALQLLDMESDLRHAIQRREFEPHFQPIVDLRDCRIVGFESLLR
                     WRHPQRGLLLPGDFLKVAEDNGSIEQIDWLMLAATCRDIPALQLDGRYVTLNVSPRRF
                     RSPALAQQLLDVLAAHDVPAHCVRIEVTEDALLENPDQVFATLDTLQRSGVRAALDDF
                     GTGYSSLSYLHRFPLHALKIDRSFVRALGPLHEGGSSAAVVRAVLALASTLGLEVIAE
                     GIETEAQREQLLELGCLRGQGFLFSPAQPAADWRAPEA"
     gene            complement(4811..6757)
                     /locus_tag="PK818_05430"
     CDS             complement(4811..6757)
                     /locus_tag="PK818_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQY82210.1"
                     /translation="MPDAGLRGAFEAANRGSLDAATLAAYSSQPLAGWLQYAALRKEL
                     ATLPTDRGGSFLARHRGQPVAEAFRNDWLAALAKRQEWAAFLSAWNDRVEQPALRCAQ
                     LEARRALGRYDERWTHDAQALWRSSGKSLPDACDPSFTQLATQGGLSPSLRWERIELA
                     AAEGQPAVIRAIARGFSGDDAAQANAYAAFIDAPVAEVDGWPKTARSRLVASHGLAKL
                     AKTSPDRAEALLPGIASVLGFNDTERGRVLHPIALWTVASYLPESARRLAAVPLPAYD
                     ASLHEWQAREAMARSDWGATLAAIRRMPDAQRTDSRWLYFAARTSELTGDAAGARTLY
                     RQAASKADFHGFLAADKLEQAYALCPWQPAVAPAAKQAIARDPAIVRALQLFALDRKG
                     WAQREWDEALSRFGDEQRRIAVEVAQDNGWFDRGVFGLVNLGGKRYPEETRLYQLRFP
                     LHHEATIRREATRNDLDPAWIAAEIRAESVFDPNARSPADARGLMQVLPGTGAEVAAK
                     IGVPWTGGQSLYDADTNIALGSAYLRQLMDKYGGKPYQVMAGYNAGPAPLGRWISQRP
                     AMDADFWIETISYKETREYVARVLSFSTVYDWRLNGDAQRLTERMLGRTQGTRKGFAC
                     PMAEAAKAAAPPPARPTLKDRRRR"
     gene            complement(6973..7710)
                     /locus_tag="PK818_05435"
     CDS             complement(6973..7710)
                     /locus_tag="PK818_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQY82211.1"
                     /translation="MLSANIQAGSSTRGYHDYVARSWSHVLPAGNKRGALDTIAELAG
                     RHDIVGLQEADPGSLRSGFTNQTHYLAERGGFAYWSHQPNRRMGGVASSANGLLSKFE
                     PREVVNHALPGRVRGRGVLLATFGNGADALSVAVAHLSLGAQSRRSQVDFIAELLGDH
                     PHAVLMGDFNCDPDRPEMQALYRHTRLQPPATCVPTFPSWKPRRAIDHVLVTPALRVA
                     MMQAMPAALSDHLALSVELDVPERAFE"
     gene            complement(7754..8611)
                     /locus_tag="PK818_05440"
     CDS             complement(7754..8611)
                     /locus_tag="PK818_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HQY82212.1"
                     /translation="MTSRLALLLLVLLPLAACKKDAPAAVDATQPGSAATAPAAQQAS
                     VQDAATSAAAEAAMRAAEEAAAKAPPPVAGVDYVEIPNGQPFDTTDGRIEIAEFFGYV
                     CPFCAAVQPTIAAMKAKLPPDVHMVYVPAAFGAMWDNYAKAYYTAEAMGLVEQTHDAM
                     FRAIHIDKTLKGERGMDTPEEIAAFYAAYGADPKQFVSSMQSFAVAAKVNRAKQYIVG
                     AFANGDSAGTPTFIVNGKYRVKGKSTDDMFRVLNQLVVAERAKRGAAAPAAAAAPTPE
                     APAAAPAQQ"
     gene            complement(8650..9324)
                     /locus_tag="PK818_05445"
     CDS             complement(8650..9324)
                     /locus_tag="PK818_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HQY82213.1"
                     /translation="MRATLLSPILSAFLLLAAAFAPAHAQRSVLPGLTEGVEYRLIEG
                     GKPYRAVPGKVEVAEVFGYWCPHCAHFAPMLEKWKASMPAHAQLVLVPAMFDPNDAYA
                     RLFFIAEGANAVNVTHDRIFRAIHQSGELPRKTTIPQLTAYYLRLPGVNPKAFTAAQK
                     DEATLQAKAANAREFQMRSKIEGTPSLVIAGRYLVLGNSYQGLLDNASRILKAIAPAA
                     KQPAAK"
     gene            complement(9407..10177)
                     /locus_tag="PK818_05450"
     CDS             complement(9407..10177)
                     /locus_tag="PK818_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQY82214.1"
                     /translation="MRLVRHGLVGVACLALAAAAYAQSTAVAVPDSAPVEVAPLDDKP
                     ATWGDAKAGQTKAGACAACHGLDGNPADPLYPRLAGMPERYIAQQLAQFRNGERTSGM
                     AALMVPFASMLSAQDMRDVGAWYATQAATSGVADDTVIASGPNQGLKFYQVGERLYRG
                     GDAARAIPACLSCHGPSGAGNPGPAYPSVKGQHAAYVARRLEEYRTGTATAKDAASFN
                     LMASVAKSLSDEEIQSLGSYVQGLHARPAGTSKAAPGH"
     gene            10303..>10826
                     /gene="yihA"
                     /locus_tag="PK818_05455"
     CDS             10303..>10826
                     /gene="yihA"
                     /locus_tag="PK818_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HQY82215.1"
                     /translation="MAVPNPFNRADYLLAAHTHRQLPPDGGREVAFAGRSNAGKSSAL
                     NALCGRNALARVSKTPGRTQQLVYFGLPPHEGKYLVDLPGYGYAKVPQDLQAHWQAFL
                     DGYFGSRMALAGLVVVMDIRHPLKDYDRSMIGYAMRRGLPAHALLTKADKLSRGAAMN
                     TVQAVRKDLSATYG"
     assembly_gap    10827..10926
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10927..>12053)
                     /locus_tag="PK818_05460"
     CDS             complement(<10927..>12053)
                     /locus_tag="PK818_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQY82216.1"
                     /translation="IRKHLDYIAGMGFTQLWPMPLLENNQPRHSYHGYAITDLYRTDP
                     RLGSNEDVRALSREAQARGVGLVMDVVLNHIGSHHWWMRDLPAKDWISNRATFTPTNH
                     RRTSIQDPHAAPADRQAFTDGWFVDTMPDLNQRNPQLARYLIQNTLWWIEYAGLSGIR
                     EDTFGYADAGFLGDWAKAVLTEYPDFAMVGEEWSTNPAVVAHWQRGNRNASGHVPHMP
                     AMMDFPVHVALRNALVQPEGWESGWIGLYEMLANDFLYPDPGALVVFAENHDTPRLLA
                     HVDGDLALWKLGMACLATVRGTPQFYYGSEVLLRGPKQRNDGLLRADMPGGWTGDAVD
                     AFGGNGLSTAQREARAYTRALFDWRKRTPLLHTGKLTHFAP"
BASE COUNT         1774 a         3996 c         4232 g         1951 t
ORIGIN      
        1 aatcgccatg ctttgcctgg cgtccttgtg caggaagcct tgcagtatcg gggtcaggtt
       61 cacttaatcg aaaaatgatg gatcacagag gcgataccgc caaccgcatc accgacatca
      121 ccaacggcgt ggactcggcg gaaacctggg acttcggcta cgacgcgttg tcgcggctgg
      181 tcaccgcgca atcgccggcc tcgctgggcg ggttcgggta cgatgccatc ggcaaccgca
      241 tcagccgcag cagcaacggc gtgcagaacg ccacgctcac ctatcccggc accagcagcc
      301 gcctgcagac ctatgccgcc agcggcctgc tactcgtagt gcgtccacat gcgtagcact
      361 tttacagtgc gctcaacctg cagcacttca tacaccagcc ggtgctggat gttgatacgc
      421 cttgaatagg cgcccgccag gtctccgacg agcttctcgt agggtggtgg cgtttgaaaa
      481 ggattctccg caaggactga cagaagtgct ttggcctttt ccttgagtcc cgaagaagcg
      541 agagtgcgtg cgtctttttg agcttgcctg gtaaagacga cgcgccaact caccagtcaa
      601 gttccgtggc gcaggcgttg gcaggctcgg ccattccgga cttgatggac tcacgcatgc
      661 caggtaccga cagcaagaac agtgtttctt gaatggccgc ccagtcctct tcggcgatga
      721 ggacggcgtt gctgcgcttg ccggaaatga caataggctc atgtgattca gccgcttgat
      781 ccatcaggcg gtagaggttg gcacgggctt cgctggctgt aagggttgtc atggctggct
      841 ccaagaatgc cgtgtaaacg tacgctaaaa cgtacgaata cgcaagtgtc agtaaaccgg
      901 aagccgagac aaaggcagct aatcaggttt gacgacctcc gcctccaatc agacctagcc
      961 actcgcgaac cggtccgtca tcgcgctcca cccaacgact tgagaatggt catgcggtgc
     1021 ctggcgtcct tgtgcaggaa gccttgcttg accagtttcc cgatcacctt gagcaccatt
     1081 tctcggctgg caccgatgcg gttggcgatt tcctgctggg tcagttcggc aggcacacgc
     1141 agcgcctgac cgtcatgcac ggcgcaggct tcaagcaggg cagaaacccg ttccggaaca
     1201 ccgtccaggg ccaggctccg ggtcatgcgg atggaatggc gaacccggga cgccagtttc
     1261 gcgaagacat gggcggcgaa ttccgggcgc gtgcgcatca ggtcctgcgc ggcgctgttg
     1321 cgcaggacca ggcactccac cgcaccgatg gcccgcaccg acgccgtgcg cggtcctccg
     1381 aacagcgaga cctcgcccag cagttcaccg ggccccagaa cggtgtagac gacttcatcg
     1441 ccgttgccgc ccgccgcgta gaccttcagt cgaccggaca acacgatgta gacgccgtcg
     1501 gacgggtcgc cttcatggaa caacacgtct ccatcgcgat agctgcgctt gcggctgtgc
     1561 ggggcaatcg gttgctgcca cggttgcggc acacgcagct gcaggtcacc catcatccct
     1621 gtccttgaac tggaatccgt tcccctgtgt ggacagggta gggcactggc acgaaagacg
     1681 cgatgcaggc accgctgccg acgcgcggac gacctgcgcc ggcgcttttc caacgtcggc
     1741 ttggcgggag gtgttgccga cgaaggccgg cgcgcccgcc agcggggatc aggcttccgg
     1801 cgcccgccag tcggccgccg gctgcgcagg cgagaacagg aaaccctgcc cgcgcaggca
     1861 gccgagctcg agcagttgct cgcgctgggc ttcggtttcg atgccttccg cgatcacctc
     1921 cagccccagt gtgctcgcaa gcgcaagcac cgcgcgcacc accgccgcgc tgctgccgcc
     1981 ttcatggagc ggccccagcg cccgcacgaa ggagcggtcg atcttcaatg catgcagcgg
     2041 gaagcgatgg aggtagctca acgaggaata gccggtgccg aagtcatcga gggccgcgcg
     2101 cacgccggag cgctgcaagg tatccagggt tgcgaacacc tggtcggggt tctccagcag
     2161 tgcatcctcg gtgacttcga tccgcacgca atgcgcgggc acgtcatggg ccgccagcac
     2221 gtcgaggagc tgctgcgcca gcgccggcga gcggaaccgg cgcggcgaga cattcagggt
     2281 gacgtagcgc ccgtccagct gcagtgccgg gatgtcgcgg caggtcgcgg ccagcatcag
     2341 ccagtcgatc tgctcgatgc tgccattgtc ctcggccacc ttcaggaaat cgcctggcag
     2401 caacagcccg cgctgcggat gccgccagcg cagcagcgat tcgaagccca cgatccggca
     2461 atcgcgcagg tcgacgatcg gctggaaatg cggctcgaac tccctgcgct ggatcgcgtg
     2521 gcgcaggtcg ctctccatgt cgagcagctg cagcgcttcc tggtgcaggc gctcgtcgaa
     2581 cagctcgaag cgctgccgcc cgtgggcctt ggcccggtac atggcgacgt cggcgtcgcg
     2641 cagcaattcc tcggccttgc ggtagcgtgg ttcgcccagg gtgatgccga cgctggcgga
     2701 cgtgaacagt tccttgccac cgacctgcat cgggccggac agcgcgtcga tcacgcgtcc
     2761 cgcggtgtgg caggcatcgt ccgcgccggc gatatccagc atcagcaccg cgaattcgtc
     2821 gccacccagg cgggccacca tgtcgccatc gcgcacgcag ccggccaacc ggccggcggc
     2881 ttccttcagc atcatgtcgc cgtagaggtg gcccacgctg tcgttgatca ccttgaagcg
     2941 gtccaggtcc aggaaaagca ccgcgaaacg atggcccgcg tcgccgtgca ggcgggccag
     3001 cgcctgctcc aggccctggt ggaaacaggt ccggttggga aggccggtca gcgcgtcatg
     3061 cagggcgtcg tgggtcaggc gttgctcggc ttgctcacgc accttgacct gctcgcgcaa
     3121 cgcacggacc accttggcca gttcgcgggt gcgatcctcc acccgacgct cgagttcggc
     3181 ctgcagcttc ttgcgttcca gcgcggtcat gatgtgctgc gccacgtagc ccagcagggt
     3241 gctgtcctcg gcgctgtagt gcacgccggg ttcgtaggtc tgcaccacca ccgcgccgcg
     3301 cgcgccgctg tcgccgctga tcggcacgcc gaggaaatcc agtgccggcg ttcccaggcc
     3361 gatgccgctg atccccaggg cccgggcgac gtcgcgtgcg gggccacgga ccggcttgcc
     3421 atggcggatc aggcccagcg tcaggctgtc gcgcaggtcc gcggccggca cctcgcgatc
     3481 ggtctcgtag atgttgacgt ccttggcgtc ggcgaagtag atgaagcgca cgctgtcgcg
     3541 ctcgccgtcg tacagcgcga tgtagaagtt gcgcgcgtag atcagctcgc cgaccatcag
     3601 gtggatgcgc cgcagcatgt ccgccatgcc cagttcgctg ctggcgaggt cggcgatccc
     3661 gtacagggcc gcctgcagac gctcgccgtg ctggcgctcg cgcacttcgt cggtgagcgc
     3721 acgcgtgcgt tcctcgaccc ggtgttcgag ttcgacgtgc gcctggtgcc ggaccagcgc
     3781 gatcaggatg tgctgggcca cgaattggag cagcgactgg tcgtccgcgc tgtagcgggt
     3841 cgccgggtcg tagctctgca ccacgatggc gccgcgcacc acgccatcgg cgatcatcgg
     3901 cacgcccagc cagtgggcac tgccgggccc gtactgttcg ttgtcctgct ccaaaccgaa
     3961 ggcctcgcgc agcgcgcgcg agggaccgag cgcaggctgg ccgctgcgga tcacccccag
     4021 ggtcaggctg ttgcggatct ccgcggccgg atattcctgc gccgggtcga ccccgtggtc
     4081 gtcctgttcg tcggcgaagt agatgaagcg caggctgtcg cgctcgcggt cgtgcagggc
     4141 gatgtagaaa ttgcgcgcat acatcagctc gccgaccaca tcgtggatgc gcgccagcat
     4201 gtcggccatc tccaggccgg ccgaggcgag gttcgccacc cggtagagcg cggtctggat
     4261 gcgcaccgtc ttttcgagcc gggtggcgac gagccgctgg tcctcgagtt ccagcgcggt
     4321 gcggacgcgc gcggccacga tcgcaaggaa gccgagccag tcggctgccg cggggtccgg
     4381 gccacccagc tcgggccatt cgacggaaag cgccaccggt tcgggcgcac cctccaggtc
     4441 gaaccaggcg cgttgcgatc cgcgcgccgc gcgacggatg tcgatcggca gcgtcgagac
     4501 cttgtggtgc ttgcgtacgg gtgctccgtc ccggcctgcc ggttgccgat gcaggcgcag
     4561 gtcgcgcacc ccgaaggagg agaccgcttc acgcgcggcg acggcctcga tgccatcgag
     4621 actggtggcg gcaaacagcg tcgacacgaa gtcgctgccc ggaccgatgc ctgagccggg
     4681 tccacccgca tcgcgcggcc ccctgtaatg gacgcgcgta gccatacgcg accgaaggta
     4741 cgcccatcac caagcccacc gcaagtgggg gtcgatcgcg gtcggccagg cgggcgcagc
     4801 ggcgcatggc tcagcggcgg cggcgatcct tcagcgtggg cctggcgggc ggcggggccg
     4861 ccgccttggc cgcttcggcc atggggcagg cgaagccctt gcgcgtgccc tgcgtgcggc
     4921 cgagcatgcg ctcggtcagc cgctgcgcat cgccgttgag gcgccagtcg tagacggtgc
     4981 tgaagctgag cacgcgcgcc acgtattcgc gggtttcctt gtagctgatc gtctcgatcc
     5041 agaaatccgc gtccatcgcc gggcgttgcg agatccagcg gcccagcggt gccgggcccg
     5101 cgttgtagcc ggccatcacc tggtagggct tgccgccgta cttgtccatc agctggcgca
     5161 ggtaggcgct gccgagcgcg atgttggtgt ccgcgtcgta caggctctgg ccgccggtcc
     5221 acggcacccc gatcttcgcg gccacctcgg cgccggtgcc gggcagcacc tgcatcagcc
     5281 cgcgcgcatc cgcgggggag cgtgcattcg gatcgaacac ggattcggcg cggatctcgg
     5341 cggcgatcca cgccgggtcc aggtcgttgc gcgtggcctc gcggcggatg gtggcctcgt
     5401 ggtgcagcgg gaagcgcagc tggtaaagcc gcgtttcctc cggatagcgc ttgccgccga
     5461 gattcaccag gccgaacacg ccacggtcga accagccgtt gtcctgcgcc acctccaccg
     5521 cgatgcgacg ttgctcgtcg ccgaaacgcg agagcgcctc gtcccactcg cgttgcgccc
     5581 agcccttgcg gtccagggcg aacaactgca gcgcgcgcac gatggccgga tcgcgcgcga
     5641 tcgcctgctt cgcggccggt gccactgccg gctgccacgg gcacagggcg tacgcttgct
     5701 cgagcttgtc ggcggcaagg aagccgtgga aatccgcctt cgatgcggcc tgccggtaca
     5761 gcgtcctggc gccggcggca tcgccggtga gctcgctggt gcgcgcggcg aaatacagcc
     5821 agcgcgaatc ggtgcgctgg gcgtcgggca tgcgccggat cgcggccagc gtggcgcccc
     5881 agtcgctgcg cgccatcgcc tcgcgggcct gccattcgtg cagcgaggcg tcgtaggccg
     5941 gcagcggcac cgccgccagc cggcgcgcgg actccggcag gtaggaggcc accgtccaca
     6001 gcgcgatcgg atgcagcacg cgaccacgct cggtgtcgtt gaagccgagc acgctggcga
     6061 tgcccggcag cagcgcttcg gcgcgatccg gcgaggtctt cgcgagtttc gccaggccat
     6121 gcgatgcgac cagccggctg cgcgcggtct tcggccagcc gtcgacctcc gccaccggcg
     6181 cgtcgatgaa ggcggcgtag gcattcgcct gcgccgcgtc gtcgccgctg aagccgcgcg
     6241 cgatggcgcg gatcaccgca ggctggcctt cggccgccgc cagctcgatc cgctcccagc
     6301 gcagcgacgg cgacaagccg ccttgcgtcg ccagttgcgt gaacgatggg tcgcaggcat
     6361 cgggcagcga cttgccgctg ctgcgccaca aggcttgcgc gtcatgcgtc catcgctcgt
     6421 cgtagcgtcc cagtgcacgc ctggcctcga gctgcgcgca acgcagcgcg ggttgctcga
     6481 cgcggtcgtt ccacgcggac aggaacgccg cccattcctg gcgtttcgcc agtgctgcca
     6541 gccagtcgtt gcggaaggcc tcggccaccg gctgcccgcg atgcctggcc aggaacgacc
     6601 cgccgcggtc ggtcggcagc gtggcaagct ccttgcgcaa cgcggcgtat tgcagccagc
     6661 cggccaatgg ctggctggaa tacgccgcca acgttgccgc gtccagcgag ccgcggttgg
     6721 cggcctcgaa ggcgccgcgc aagccggcgt ccggcatcgc gatcagtacc ggtgggttgg
     6781 cggcgcgcgg cggcggcacc ggtgcctgcg cgcgggcgct gccgcttgcg cacaggttgg
     6841 ccagcagcag gccgagcgtg gccagcgtga gcgtgtgcat cgccggacta tagccgagcg
     6901 tttctgaacc cgtttttcgc ggaccggcga tcgcccatga ccgctgctgc tgcgccgggc
     6961 cggcggcgcg gatcactcga aggcgcgttc cggcacgtcc agttcgaccg acagcgcaag
     7021 gtggtccgag agcgcagccg gcatcgcctg catcatcgcc acccgcagcg ccggcgtgac
     7081 cagcacgtgg tcgatcgcgc gccgcggctt ccacgaggga aacgtcggca cgcaggtcgc
     7141 cggcggttgc agccgggtgt ggcggtacag cgcctgcatc tccgggcggt ccggatcgca
     7201 gttgaaatcg cccatcagca ccgcgtgcgg gtggtcgccc agcagttcgg cgatgaagtc
     7261 gacctgcgag cgccgcgact gcgcgcccag cgagaggtgc gcgaccgcga ccgacagggc
     7321 atcggcaccg ttgccgaagg tcgccagcaa cacgccgcgc ccgcgcacgc ggccgggcag
     7381 cgcgtggttc acgacttcgc gcggctcgaa cttgctgagc aggccgttgg cgctggacgc
     7441 caccccgccc atgcgccggt tcggctggtg gctccagtag gcgaagccgc cgcgttcggc
     7501 caggtaatgg gtctggttgg tgaagccgga gcgcaggctg ccggggtcgg cttcctgcag
     7561 gccgacgatg tcgtggcggc ccgcgagttc ggcgatggtg tcgagcgcgc cgcgcttgtt
     7621 gccggcgggc agcacgtgcg accagctgcg ggcgacgtag tcgtggtagc cgcgcgtgct
     7681 ggagccggcc tggatgttgg cgctgagcag gcgcagccgg cgctgggtca tgccggcggc
     7741 ctgcggcgat ggatcactgc tgcgcgggcg cggccgcggg cgcttcgggc gtcggtgccg
     7801 cggcggcggc cggtgcggcc gcaccccgct tggcgcgttc ggccaccacc agctggttga
     7861 ggacacggaa catgtcgtcg gtggacttgc ccttgacccg gtacttgccg ttgacgatga
     7921 aggtcggggt gcccgcgctg tcgccgttgg cgaacgcgcc cacgatgtac tgcttggccc
     7981 ggttgacctt ggcggcgacc gcgaagctct gcatgctgct gacgaactgc ttggggtcgg
     8041 cgccgtacgc ggcgtagaac gcggcgatct cttccggggt gtccatcccg cgctcgccct
     8101 tcagggtctt gtcgatgtgg atggcgcgga acatggcgtc gtgggtctgc tcgaccaggc
     8161 ccatcgcctc ggcggtgtag taggccttgg cgtagttgtc ccacatcgcg ccgaacgcgg
     8221 cgggaacgta gaccatgtgc acgtccggcg gcagcttggc cttcatcgcg gcgatggtgg
     8281 gctgcaccgc ggcgcagaac gggcagacat agccgaagaa ctcggcgatc tcgatgcggc
     8341 cgtcggtggt gtcgaacggc tgcccgttgg ggatctcgac gtagtcgacg cccgccaccg
     8401 gcggcggtgc cttggccgcg gcttcttcgg cagcgcgcat ggcggcctct gcggcggcgc
     8461 tggtggcggc gtcctggacg ctggcctgct gtgcggcggg ggcggtggcg gcggaacccg
     8521 gttgcgtggc atcaaccgcg gccggcgcgt ccttcttgca ggcggcgagc ggcaacagga
     8581 ccaggagcag gagggcgagg cgcgacgtca tggcgggtat ccgtggatgc tgggttggcg
     8641 ggcaggcggt tacttggcgg cgggttgctt ggctgccggc gcgatggcct tgaggatgcg
     8701 gctggcgttg tcgagcaggc cctggtagct gttgcccagc accaggtagc ggcccgcgat
     8761 caccagcgaa ggagtgccct cgatcttgct gcgcatctgg aactcgcgcg cgttcgcggc
     8821 ctttgcctgc agggtggctt catctttctg cgctgccgtg aaggccttgg ggttcacgcc
     8881 gggcaagcgc aggtagtagg cggtgagctg cgggatcgtg gtcttgcgtg gcagttcgcc
     8941 gctttggtgg atcgcgcgga agatgcggtc atgcgtcacg ttgacggcat tggctccctc
     9001 ggcgatgaag aacaggcgcg cgtaagcgtc gttcgggtcg aacatcgcgg gtaccagcac
     9061 aagttgcgcg tgcgccggca tgctcgcctt ccacttttcc agcatcggcg cgaaatgcgc
     9121 gcaatgcggg caccagtacc cgaacacttc ggccacttcg accttgccgg gcaccgcgcg
     9181 atacggcttg ccaccctcga tcagccggta ttccacgccc tcggtgaggc cggggagcac
     9241 gctacgctgt gcatgcgccg gggcgaacgc cgcggccagc aacaggaacg ccgacaggat
     9301 cggggacagc agggttgcgc gcatcggatc aggcctcttg gcaggggtgt cggcggacga
     9361 gcatgcgtgc gtccgcctca atgcccgatg aagcggtcgc tgcaggtcag tgaccgggtg
     9421 cggccttgga agttccggcg ggacgcgcgt gcaggccctg tacgtagctg cccagcgact
     9481 ggatttcctc gtcgctgagc gacttggcca cgctcgccat caggttgaag gacgcggcgt
     9541 ccttcgcagt ggcggtgccg gtgcggtatt cctccagccg gcgcgccacg taggcggcat
     9601 gctggccctt caccgaggga taggccggac ccgggttgcc ggcgccggag gggccgtggc
     9661 aggacaggca ggcgggaatg gcgcgggcgg cgtcgccacc gcggtagagg cgctcgccga
     9721 cctggtagaa cttcaggccc tggttggggc cgctggcgat cacggtgtcg tcggcaaccc
     9781 cggaggtggc cgcctgggtg gcgtaccagg cgccgacgtc gcgcatgtcc tgcgccgaca
     9841 gcatgctcgc gaacggcacc atcagcgcgg ccatgccgct ggtgcgctca ccgttcctga
     9901 actgggcgag ctgctgggcg atgtagcgct ccggcatccc ggccaggcgc gggtacagcg
     9961 ggtccgcggg gttgccgtcg aggccgtggc aggccgcgca cgcgccggcc ttggtctgcc
    10021 cggccttggc atcgccccag gtagccggct tgtcgtccag cggcgcgact tcgaccgggg
    10081 cgctgtcggg cacggcgacg gccgtgctct gggcgtaggc ggccgcggcc agtgcgaggc
    10141 aggcaacgcc gacgagacca tggcggacaa gacgcatgcg gaactccacg ctattgggtg
    10201 ggcggcagga cggccgctga accggggaat tatcgtcgcg ccgcacaggg tggtcaaacc
    10261 ggcgtggggc ggtcgcgctg gccgacgtgg catcctatgc ccatggccgt ccccaatccg
    10321 ttcaaccgcg ccgattacct gctggccgcg cacacccacc gccagctgcc gccggacggt
    10381 ggccgcgagg tcgccttcgc cggccgttcc aacgccggca agtcgtcggc gctgaacgcg
    10441 ctgtgcgggc gcaatgcgct ggcgcgggtg tccaagacgc cgggccgcac ccagcaactg
    10501 gtgtacttcg ggctgccgcc gcacgagggc aagtacctgg tcgacctgcc gggctacggc
    10561 tacgccaagg tgccgcagga cctgcaggcg cactggcagg cgttcctcga tggctatttc
    10621 ggcagccgga tggcgctggc cgggctggtg gtggtgatgg acatccgcca cccgctgaag
    10681 gactacgacc gctcgatgat cggctatgcg atgcgtcgcg gcctgcccgc gcacgcgctg
    10741 ctgaccaagg ccgacaagct ctcgcgcggg gcggcgatga acaccgtgca ggcggtccgc
    10801 aaggacctgt cggcgaccta cggcgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnncggc gcgaagtggg tcagcttgcc ggtgtgcagc agcggggtgc gcttgcgcca
    10981 gtcgaacagc gcacgggtgt aggcccgcgc ttcgcgctgc gcggtgctca ggccattccc
    11041 gccgaaggca tccacggcat cgccggtcca gccgccgggc atgtcggcgc gcagcaggcc
    11101 gtcgttgcgt tgcttcgggc cacgcagcag cacttcggaa ccgtagtaga actgcggggt
    11161 gccgcgcacg gtggcgaggc aggccatgcc gagtttccac agcgccaggt cgccgtcgac
    11221 gtgcgccagc aggcgcgggg tgtcgtggtt ctcggcgaac accaccagtg cgccggggtc
    11281 cgggtacagg aaatcgttgg ccagcatttc gtacaggccg atccagccgc tctcccagcc
    11341 ctcgggctgc accagcgcgt tgcgcagggc gacgtggacg gggaagtcca tcatggcggg
    11401 catgtgcggg acgtggccgc tggcgttgcg attgccgcgc tgccagtggg ccaccactgc
    11461 cggattggtg ctccattcct cgccgaccat cgcgaagtcc gggtactcgg tcagcactgc
    11521 cttcgcccag tcgccgagga agcctgcgtc ggcgtaaccg aaggtgtcct cgcggatgcc
    11581 ggacagcccg gcgtactcga tccaccacag cgtgttctgg atcaggtagc gcgccagttg
    11641 cgggttgcgc tggttgaggt ccggcatcgt gtccacgaac cagccatcgg tgaaggcctg
    11701 gcggtcggcg ggtgcggcgt gcggatcctg gatgctggtg cggcgatggt tggtcggcgt
    11761 gaacgtcgcg cggttgctga tccagtcctt cgccggcagg tcgcgcatcc accagtggtg
    11821 cgagccgatg tggttgagca ccacgtccat cacaaggccg acgcccctcg cctgcgcctc
    11881 gcgcgacagc gcacgcacgt cctcgttgct gcccagccgc gggtcggtgc ggtagaggtc
    11941 ggtgatcgcg tagccgtggt acgaatgcct gggctggttg ttttccagca gcggcatcgg
    12001 ccacagctgg gtgaagccca tgccggcgat gtagtccagg tgcttgcgga tgc
//
LOCUS       DAORUK010000057        12001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_5404_length_12001_cov_18.575506, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000057 DAORUK010000000
VERSION     DAORUK010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 12001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12001
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5404_length_12001_cov_18.575506"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PK818_05465"
     CDS             complement(<1..595)
                     /locus_tag="PK818_05465"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQY82217.1"
                     /translation="MDSSLKQRLVGAVVLIALAVIFLPMLVKGPAPDSGVSDVPLGIP
                     GEPKAADTTTRDLPLIEPGTAPKGGAVGMPEATPESANPGPAQDGQFAAAAAGDYAVS
                     FGSYASAADADKVIAALRSAELPAYREAVQLAGRDAQRVRIGPFTDHAMAEAARLRAA
                     QVNDGVGAKVVVLDAGMPEVAPAAAAIAAPAVAVAKPG"
     gene            complement(655..1920)
                     /gene="folC"
                     /locus_tag="PK818_05470"
     CDS             complement(655..1920)
                     /gene="folC"
                     /locus_tag="PK818_05470"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HQY82218.1"
                     /translation="MPRTLADWLAHIERQHPKSIDMGLQRVREVAARMRLPRPAKRVI
                     TVGGTNGKGSTVAFVEAIARAHGWKVGCYTSPHLLRYNERVRIDGVDAGDAALVAGFE
                     AVEAARGDTPLTYFEYGTLCALWLFARSKLDLAVLEVGLGGRLDAVNVVDADASIITT
                     VDLDHQDWLGEDIEAIGFEKAGIARPFKPLVLGDDDPPASVLRHAYAIGAQSWRIAND
                     FFAEPLDAETWRWREVGFSMDLPMPALAAPVQLRNAACAIAAVRTLRARIDKQTYAEG
                     VANARVAGRLQRFERNGVEVVVDVGHNPQAARALAAWLRAQPRRRTLAVYAALADKDV
                     AGVAQALAGVVDGWHIAGLSDAGPRGLGVDDFARQLAGTAAADAARHVGVDAALQAAM
                     AQAGPGGRVLVFGSFHTAAAALGHLGADG"
     gene            1977..>2764
                     /locus_tag="PK818_05475"
     CDS             1977..>2764
                     /locus_tag="PK818_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82219.1"
                     /translation="MQAFIRSVLAAAILLLGAGCASLPPAAAGDAASGFREVRNQPHY
                     RVSVRDGTAAWPTPPLFRLPMVEVASDALDTAISPAQGGVVATHAARTVCLALAPWLQ
                     WTSADEGGRVALQVRRVAASSSGLAATSAVIDAVVPGPFRLPAGLGALRVDTRIEDGA
                     GAALLELQWERGANPLLHRARASSIGDAWELAPSHARDLEEVLAPLKLAKQAEPVRKA
                     NIATCEQRFGRVSVAGRAASMFVPLSPEAIDPSDDAPRTAPQPQP"
     assembly_gap    2765..2864
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2891..3541)
                     /locus_tag="PK818_05480"
     CDS             complement(2891..3541)
                     /locus_tag="PK818_05480"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQY82220.1"
                     /translation="MRILLARHGETPWNAEGRYQGQEDIPLSAVGIAQATALGERLRA
                     LRIDRALASPLSRARRTAELALGEGRASQLLLDDGFKEIGHGEWEGLLASEIRERDGE
                     RLQAWREAPDTVQMPGPGGESLKQVLDRAWPAFARACAGLGEADTLLVVAHDAVNRVL
                     LCRVLGVPLAKLWTFRQAPTTLNLLEGAGVDALEVVRLNDCSHHTALFGEAVHRAL"
     gene            complement(3544..5106)
                     /gene="serA"
                     /locus_tag="PK818_05485"
     CDS             complement(3544..5106)
                     /gene="serA"
                     /locus_tag="PK818_05485"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HQY82221.1"
                     /translation="MKVLACDGIHEDGLALFREAGWDVLVSPPIKDPEALKLALADAD
                     AVLVRSATQVTLESLADAGRLKVIGRAGAGVDTIDVDAATARGIAVMNAPDGNTLAAA
                     EHAISLLFALARHIPRADAGMKAGQWPKAGLTGFELEGKKLGVIGLGRIGGTVARKAA
                     GIGMDVAAFDPFLPPSAAAKGSVPLKTLDELLAWADIVTLHIPRTKDTTNLLSEQRMR
                     AMKPGAYLVNAARGGLVDEAALLDLLDDGHLAGAALDTFATEPLQEDSPLRAHPKLIL
                     TPHLGASTSEAQQAVSTILARQVIDFVATGAVAGCVNLPPLTAEAAREVGPWMPLMTA
                     LGKLAARLVQAPTRLDITYAGRTDALDTRPLTRLLVASLLGAHSGRVTPVNALAEAAA
                     RGLVVAETVGGDGDGFDRLLKLRVSDGNRSREIEATLHRGPRVVRLDGVEIEFDPQSH
                     VLLLRNEDRPGMVGAVGSKLGELGINIVNFALGAAGDGQARAAITVDRALDETQLANL
                     RAMPGVLSLAQA"
     gene            5359..5751
                     /locus_tag="PK818_05490"
     CDS             5359..5751
                     /locus_tag="PK818_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HQY82222.1"
                     /translation="MQAKGEFEVKRIPQEELDIGEGATVGHSRFDKRFHGPLDATSVV
                     HMLAVMSAVPGSGAYVAMERIEGTLDGRRGSFFAQHNGVMDRGKPTLDLTVVPDTGTG
                     DLTGLHGRIAIDIVDGKHSYTFDYDFRE"
     gene            complement(5815..7998)
                     /locus_tag="PK818_05495"
     CDS             complement(5815..7998)
                     /locus_tag="PK818_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HQY82223.1"
                     /translation="MRKTMLATGITVAMVGTALVGAPRSANAGEGMWVPQQLPEIAGP
                     LKKAGLRLDPKQLADLTGDPMGAVVSLGGCTASFVSPRGLVVTNHHCAYGAIQLNSTP
                     DKNLIRDGFNAPAAGDELPAGPNARIYVLDQITDVTAQVKSAIAAAPGPLERTRAVED
                     LEKKLVGECEAEAGYRCRLYSFMGGNAYRLFKNIEIKDVRLAYAPPGSVGNYGGEVDN
                     WMWPRHTGDFAFYRAYVGKDGKPAAYGKDNVPFTPKRWLQVADKPLGEGDFVMVAGYP
                     GSTARYALAADFDATSGWTYPTVSRRYKALAAMVHAAGAKNPDIAVKYASTVKGWENT
                     LKNYDGQLEGFARMGAAGIKREREQAVLDWLKSRGADGAQALAAHASLLALGEQARKT
                     RERDSVIGNVGGALGSSALTLYRLAVEREKPDAQRESGFQQRDLPGIEGGMKQMDRRY
                     VAEMDRQLQRYWLLEYIALPANQRIAAIDAWLGGNDAAAVDAALDRINASKLGSVDER
                     MKWLTADRKAFEASTDPAIQYAVAMLPTSLKLEEDRKLRAGEMIVPRATYLQAVADYN
                     KAQGKAVYPDANSSLRITFGNVMGYTKPGSSKAEDAFTTLEQVAAKATGKEPFDAPQV
                     QLDAIKARTYAGLADARLKTVPVNFLSDLDITGGNSGSPVLDAHGKLVGLAFDGNWES
                     VASNWVFDPTMSRMISVDQRYMRWVMQEVMPAPQLLQEMGVAPKQ"
     gene            complement(8061..10211)
                     /locus_tag="PK818_05500"
     CDS             complement(8061..10211)
                     /locus_tag="PK818_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019337940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HQY82224.1"
                     /translation="MRLRPALLTLALAMAGGAAHADEGMWMPSQLPQLAKELKAAGFR
                     GNPADLANLTRHPMSAVVSLGGCTASFVSPQGLVVTNHHCALGAIQLNSSAERNLIQD
                     GFIAATQADEPSAGPAARVLVTVGFDQVTDRILAEARGKTGRAYYDAVQAAQKATVAA
                     CEQDAGHRCSVASMYYGREYYLIKQLELKDIRLAYAPPRAIGNYGDEVDNFMWPRHTG
                     DFTFYRAYVGKDGKPAAYSKDNVPYAPPAHLEVSTAPVRAGDFAMVAGYPGTTFRHRT
                     ASEFANQIDWLLPKRIEVVGGMLQAIEAATAGDKARQVLYASTVAGQKNTLKRAQGEL
                     DGLRRSDAVRVRAADEAAMLAWLAKQPDAVAVQADIRALQAQLDAVQTNRERDLLLPF
                     LRPGLLGAAISLQRLSLERGKPDVQRESGYQQRDEALIEGGLKQLQRRFDPEVEKATL
                     RYALKGYFALPRALRVAEFDVVFGASEAEAAQRLDALYAGTKLGDEATRLAAMQLDAA
                     ALAASEDALLKAAATLQPALLRLEAESKQRNGELMRLRPAYMQALVGYRNAQGRAVYP
                     DANATLRVSYGKVSPLSPRDGMDYRPLTTVAGIVEKDTGVEPFDAPKPLIDAIAKGDF
                     GSTLDPTLGTQTVNFMTNLDTTGGNSGSPVLDANGKLIGLNFDSNWEAVSASWMFDPR
                     YKRAIHVDMRYLRWLLAKVYPAPHLLKEMNLPAE"
     gene            10347..11384
                     /gene="tdh"
                     /locus_tag="PK818_05505"
     CDS             10347..11384
                     /gene="tdh"
                     /locus_tag="PK818_05505"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017164734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HQY82225.1"
                     /translation="MSQATMKALVKREAAKGIWMEQVPVPSPGPNEVLIKLEKTAICG
                     TDLHIYLWDEWSQRTIKPGLVIGHEFVGRIVGIGPGVSGYAPGQRVSAEGHIVCGHCR
                     NCRAGKQHLCPNTVGIGVNRNGAFAEYIVMPASNLWPIPDQIPSELAAFFDPYGNAAH
                     CALEFDVVGEDVLITGAGPIGIIAAGICKHIGARNVVVTDVNDYRLKLAADMGATRVV
                     NVANTSLKDVMSDLHMEGFDVGLEMSGNPRAFNDMLDCMYNGGKIAMLGIMPKGAGVD
                     WDKIIFKGLTLQGIYGRRMYETWYKMTQLVLSGFPLGKVLTHQLPIDDFQKGFELMES
                     GKAGKVVLSWN"
     gene            11530..>12001
                     /locus_tag="PK818_05510"
     CDS             11530..>12001
                     /locus_tag="PK818_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQY82226.1"
                     /translation="MSLTARYADTLDEIRAAGLFKSERIITSPQSAEITLADGRTVLN
                     FCANNYLGLADHPDVIAAAKDALDTHGFGMASVRFICGTQDLHKQLEKTIADFFGTED
                     TILYAACFDANGGLFEPLLDENDAIISDALNHASIIDGVRLCKARRYRYANCDMA"
BASE COUNT         1744 a         4284 c         4036 g         1837 t
ORIGIN      
        1 acccgggctt cgccaccgcc actgccggtg cggcgatcgc ggccgctgca ggcgccacct
       61 ccggcatgcc cgcatccagc accaccacct tggcgccgac gccatcgttg acctgcgccg
      121 cgcgcaggcg cgcggcctcg gccatcgcgt ggtcagtgaa cgggccgatg cgcacccgct
      181 gggcgtcgcg gccggcgagc tgcaccgcct cgcgataggc cggcagctcg gcgctgcgca
      241 acgcggcgat caccttgtcc gcatccgcgg cactggcgta gctgccgaag ctgaccgcgt
      301 agtcgccggc cgccgccgcg gcaaactggc cgtcctgcgc gggacccggg ttggcggact
      361 cgggcgtggc ttccggcatc ccgaccgcgc cgcccttcgg cgcggtgccg ggctcgatca
      421 gcggcaggtc gcgggtggtg gtgtcggcgg ccttcggctc gccagggatc cccagcggca
      481 cgtcggagac gccgctgtcg ggcgccgggc ccttgaccag catcggcagg aagatgaccg
      541 ccagcgcgat cagcacgacc gcgccgacca ggcgttgctt cagggaagaa tccatcgacg
      601 gggaaccggc gcggggaatg cgcgaattat agcggccggc gccaatgccc cggcttaacc
      661 gtccgcgccg aggtgcccca gcgcggccgc cgcggtgtgg aaggagccga acaccagcac
      721 ccgcccgccc ggcccggcct gcgccattgc ggcctgcaac gcggcgtcga ccccgacatg
      781 gcgcgccgca tccgccgccg ccgtcccggc cagctgccgc gcgaagtcat cgacgcccag
      841 cccgcgcgga cccgcgtcgg acaggccggc gatgtgccag ccatccacca ccccggccag
      901 cgcctgcgcc acccccgcca cgtccttgtc ggccagcgcg gcatacaccg ccagcgtgcg
      961 ccgccgcggt tgcgcgcgca gccacgccgc cagcgcgcgc gcggcctgcg ggttgtggcc
     1021 gacatcgacc accacctcga caccgttgcg ttcgaaacgc tgcaaccgcc cggccacgcg
     1081 ggcattggcg acgccctcgg catacgtctg cttgtcgatg cgcgcacgca gagtacgcac
     1141 ggcggcgatc gcgcaggcgg cgttgcgcag ttgcaccggc gcggccaagg ccggcatcgg
     1201 caggtccatc gagaacccga cctcgcgcca gcgccaggtt tcggcatcca gcggttccgc
     1261 gaagaaatcg ttggcgatcc gccaggactg cgcgccgatc gcatacgcat gccgcagcac
     1321 gctggccggc gggtcgtcgt cgcccagcac cagcggcttg aacgggcgcg cgatgccggc
     1381 tttctcgaag ccgatcgcct cgatgtcctc gcccagccaa tcctggtggt cgagatcgac
     1441 ggtggtgatg atcgatgcat ccgcatcgac gacgttgacc gcatccaggc gcccgcccag
     1501 gccgacttcc agcaccgcga gatccagctt gctgcgcgcg aacagccaca gcgcgcacag
     1561 cgtgccgtac tcgaaatagg tcagcggggt gtcgccgcgc gcggcctcca ccgcctcgaa
     1621 gccggcgacc agcgcggcat cgccggcatc cacgccgtcg atgcgcacgc gctcgttgta
     1681 gcgcagcagg tgcggcgagg tgtagcagcc caccttccag ccgtgggcgc gggcgatcgc
     1741 ctcgacgaag gccacggtgg agcccttgcc gttggtgccg ccaacggtga tcacgcgctt
     1801 cgccgggcgc ggcagccgca tccgcgcggc gacctcgcgc acgcgctgca ggcccatgtc
     1861 gatggacttc gggtgctggc gctcgatgtg cgccagccaa tcggcaaggg tgcgtggcat
     1921 ccgcggatta tccgtgccgc tgcatccgcc ggcgagcccg cggcgtataa cgcgccatgc
     1981 aagccttcat tcgatctgtc ctggccgcgg caatcctcct gctcggcgca ggttgcgcca
     2041 gcctgccgcc agccgctgcc ggcgacgccg ccagcggctt ccgcgaggtg cgcaaccagc
     2101 cgcattaccg ggtgtcggtg cgcgatggca ccgccgcctg gccaaccccg ccgctgttcc
     2161 gcctgccgat ggtggaggtt gccagcgacg ccctcgacac cgcgatctcg cccgcgcagg
     2221 gcggcgtggt ggcgacccat gccgcgcgca ccgtgtgcct ggcactggcg ccgtggctgc
     2281 aatggaccag cgccgatgaa ggcggccggg tcgcgctgca ggtgcgccgg gttgcggcca
     2341 gcagcagcgg actggcggcg acgtcggcgg tgatcgacgc ggtggtgcct ggcccgttcc
     2401 ggctgccggc cgggttgggc gcattgcggg tcgacacccg catcgaggac ggcgcaggcg
     2461 cggccttgct ggaactgcag tgggaacgcg gggccaatcc gctgttgcac cgcgcccgcg
     2521 cgtcctcgat cggggatgcg tgggaactcg cgccctcgca tgcccgcgac ctggaggagg
     2581 tgctggcacc gctgaagctg gccaagcagg cggagccggt gcgcaaggcc aacatcgcca
     2641 cctgcgaaca acgcttcggg cgggtcagcg tggccggccg cgccgcttcg atgttcgtgc
     2701 cgctgtcgcc ggaggcgatc gatccctccg acgatgcgcc ccgaaccgcc ccgcaaccgc
     2761 aaccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccgcaa ccgcaaccaa
     2881 atcccgggcc ctagagtgcg cggtgcaccg cttcgccgaa cagcgcggtg tggtggctgc
     2941 agtcgttcag gcgcacgacc tccagcgcat ccacgcccgc accttccagc aggttgagcg
     3001 tggtcggcgc ctgccggaag gtccacagct tcgccagcgg aacgccgagc acccggcaca
     3061 gcagcacccg gttgaccgcg tcatgcgcca ccaccagcag ggtgtcggcc tcacccaatc
     3121 ccgcacatgc gcgggcgaag gccggccagg cgcggtccag cacctgcttc agcgactcgc
     3181 cgcccggccc tggcatctgc accgtatccg gggcctcgcg ccaggcctgc aggcgttcgc
     3241 cgtcgcgctc gcggatttcg ctggcaagca ggccctccca ctcgccgtgg ccgatttcct
     3301 tgaacccgtc atcgagcagc agttgcgatg cgcgcccctc gccaagcgcc agctcggcgg
     3361 tacggcgtgc gcgcgacagc ggcgaggcca gcgcgcggtc gatccgcagc gcgcgcaggc
     3421 gctcacccag ggcggtcgcc tgggcgatgc cgaccgcgga caacgggatg tcctcctggc
     3481 cctggtagcg gccttcggcg ttccacggcg tttccccgtg gcgtgcaagc aggatgcgca
     3541 tgctcaggcc tgcgccagcg agagcacgcc gggcatcgcg cgcaggttgg ccagctgggt
     3601 ttcgtcgagc gcacgatcga ccgtgatcgc cgcccgggcc tggccatcgc cggccgcgcc
     3661 cagcgcgaaa ttgacgatgt tgatcccgag ctcgccgagc ttcgagccaa ccgcgccgac
     3721 catgcccggc cggtcctcgt tgcgcagcag cagcacgtgc gactgcgggt cgaattcgat
     3781 ctccacgccg tccagccgga ccacgcgcgg accgcgatgc agggtggcct cgatctcgcg
     3841 gctgcggttg ccgtcgctca cccgcagctt gagcaggcgg tcgaaaccgt cgccgtcgcc
     3901 acccacggtt tcggccacca ccaggccgcg cgcggcggct tcggccagtg cgttcaccgg
     3961 ggtgacccgg ccggaatgcg cgcccagcag ggaggccacc agcagccgcg tcagcgggcg
     4021 cgtgtccagc gcgtcggtgc ggccggcgta ggtgatgtcc agccgcgtcg gtgcctgcac
     4081 caggcgcgcg gcgagcttgc ccagtgcggt catcagcggc atccacggcc ccacttcgcg
     4141 cgccgcctcg gcggtcagtg gcggcaggtt gacgcagccg gcgaccgcgc cggtggcgac
     4201 gaagtcgatc acctgccgcg ccaggatcgt gctcaccgcc tgctgggctt cgctggtgga
     4261 agcgcccagg tgcggggtca ggatcagctt cgggtgcgcg cgcagcggcg aatcctcctg
     4321 cagcggttcg gtggcgaagg tgtccagcgc cgcgccggcg aggtggccgt cgtcgagcag
     4381 gtccagcagt gccgcctcgt cgaccaggcc cccgcgtgcg gcgttgacca ggtaggcgcc
     4441 cggcttcatc gcgcgcatgc gctgttcgga cagcaggttg gtggtgtcct tggtgcgcgg
     4501 gatgtgcagg gtcacgatgt cggcccacgc cagcagttca tccagcgtct tcagcggcac
     4561 gctgcccttg gcggccgcgg acggcggaag gaacgggtcg aacgcggcca cgtccatgcc
     4621 gatgccggcg gccttgcgcg ccaccgtgcc gccgatgcgg cccagcccga tcacgcccag
     4681 cttcttgcct tccagttcga acccggtgag gcccgccttc ggccactgcc cggccttcat
     4741 cccggcatcc gcgcgcggga tgtggcgggc cagcgcgaac agcagcgaga tcgcgtgctc
     4801 ggcggcggcc agggtgttgc cgtccggggc gttcatcacc gcgatgccgc gcgcggttgc
     4861 cgcgtcgaca tcgatggtgt cgacgccggc accggcgcgg ccgatcacct tcagccggcc
     4921 ggcatcggcc agtgactcca acgtgacctg ggtcgccgag cgcaccagca cggcatcggc
     4981 atcggccagc gccagcttca gcgcctccgg atccttgatc gggggggaga ccagcacgtc
     5041 ccagcccgcc tcgcggaaca gggccaggcc gtcttcatgg attccatcgc acgccagcac
     5101 tttcatcaca tcgctcctgt ggggttgatt cctgaccctg ggtcaggtcc cgcggaagcc
     5161 atggcagccg gcacagcgca atcgacttgc tggaagtgcg aagaacagcg gaatcacggc
     5221 gaaaggcacg ggattccact tgcggtcggg agacggggcc ggctggcatg ggaatccgcg
     5281 acacccgcag cctacagagt tcaccgcggg ttgtgcaacc cgggctacgc tgcccctcca
     5341 ccccacggga taccggtcat gcaggcaaag ggcgagttcg aggtcaagcg catcccgcag
     5401 gaggagctgg acatcggcga gggcgcgacg gtcggccact cacgcttcga caagcgtttc
     5461 catggcccgc tggacgccac cagcgtggtc cacatgctcg ccgtgatgtc cgcggtgccg
     5521 gggtccggcg cctacgtggc gatggagcgc atcgagggca cgctggacgg ccgccgcggc
     5581 agcttcttcg cccagcacaa cggggtcatg gaccgcggca agcccaccct ggacctgacc
     5641 gtggtgcccg acaccggcac cggcgatctc accggcctgc acgggcgcat cgccatcgac
     5701 atcgtcgacg gcaagcattc ctacaccttc gattacgatt tccgcgagtg acgccgcgca
     5761 aaaggaaacg ccggcacggg gccggcgctt ccggggcgcg cactggcagt cgcgttactg
     5821 cttcggcgcg acgcccattt cctgcagcag ctgcggcgcc ggcatcactt cctgcatgac
     5881 ccagcgcatg tagcgctggt ccaccgagat catgcgcgac atcgtcgggt cgaacaccca
     5941 gttcgaggcc accgattccc agttgccgtc gaacgccagg ccaaccagct tgccgtgcgc
     6001 gtccagcacc ggagaaccgg aattgccgcc ggtgatgtcg aggtcggaca ggaagttcac
     6061 cggcaccgtc ttcagccggg catctgccag gccggcatac gtcctcgcct tgatcgcatc
     6121 cagctgcacc tgcggggcat cgaatggctc cttgccggtc gccttcgccg cgacctgctc
     6181 cagcgtggtg aacgcgtcct cggccttgct gctgccgggc ttggtgtagc ccatcacgtt
     6241 gccgaaggtg atccgcagcg aggagttcgc atccgggtac accgccttgc cctgcgcctt
     6301 gttgtagtcg gccacggcct gcaggtaggt cgcacgcggc acgatcattt cgccggcgcg
     6361 cagcttgcgg tcttcctcca gcttcagcga ggtcggcagc atcgccaccg cgtactggat
     6421 ggccgggtcg gtgctggcct cgaacgcctt gcggtccgcc gtgagccact tcatgcgttc
     6481 atcgacgctg ccgagcttgc tggcgttgat gcggtccagc gcggcatcca ccgccgcggc
     6541 gtcgttgccg cccagccatg cgtcgatcgc ggcgatccgc tggttggccg gcagcgcgat
     6601 gtactccagc agccagtagc gctgcagctg gcgatccatc tccgcgacgt agcggcgatc
     6661 catctgcttc atgccgccct cgatgcccgg caggtcgcgc tgctggaagc cggactcgcg
     6721 ctgcgcgtcc ggcttttcgc gctccaccgc gagccggtac agggtcagcg cgctgctgcc
     6781 cagcgcgccg ccgacgttgc cgatcacgct gtcgcgctcg cgggtcttgc gcgcctgctc
     6841 gcccagtgcc agcagggagg cgtgcgcggc cagtgcctgc gcgccatcgg cgccgcgcga
     6901 cttcagccag tccagcaccg cctgctcgcg ttcgcgcttg atcccggcgg cgcccatgcg
     6961 ggcgaagccc tccagttggc cgtcgtagtt cttgagcgtg ttctcccagc ccttgaccgt
     7021 gctcgcgtac ttcactgcga tgtccggatt cttcgcgccg gcggcgtgca ccatcgccgc
     7081 cagcgccttg tagcgcctgc tgacggtggg ataggtccag ccgctggtgg catcgaaatc
     7141 ggcggccagc gcatagcgcg cggtgctgcc cggatagccg gccaccatca cgaagtcgcc
     7201 ctcgcccaac ggcttgtccg cgacctgcag ccagcgcttg ggggtgaacg gcacgttgtc
     7261 cttgccgtag gccgcgggct tgccgtcctt gccgacgtag gcgcggtaga acgcgaagtc
     7321 gccggtgtgg cgcggccaca tccagttgtc gacctcgccg ccgtagttgc ccacgctacc
     7381 gggcggtgcg taggccaggc gcacgtcctt gatctcgatg ttcttgaaca ggcggtaggc
     7441 attgccgccc atgaagctgt acaggcggca gcggtagccg gcctcggcct cgcattcgcc
     7501 gaccagcttc ttctccaggt cctcgaccgc gcgggtgcgc tccagcggac cgggggcggc
     7561 agcgatcgcg gacttcacct gcgcggtgac gtcggtgatc tggtcgagca cgtagatgcg
     7621 cgcattcggg ccggccggaa gttcatcgcc cgcggcaggc gcgttgaagc cgtcgcggat
     7681 caggttcttg tccggcgtgg aattgagctg gatcgcgcca taggcgcagt ggtggttggt
     7741 gaccaccagg ccccgcggcg acacgaagct cgcggtgcag ccgcccagcg agaccaccgc
     7801 gcccatcggg tcgccggtga ggtccgccag ttgcttcgga tccagcctca gcccggcctt
     7861 cttcagcgga ccggcgattt ccggcaactg ctgcggcacc cacatgccct cgcccgcgtt
     7921 cgcggagcgc ggcgcgccca ccagtgcggt tcccaccatc gccacggtga ttcccgtggc
     7981 cagcatcgtc ttgcgcattg tcgtttcctt gcagtgttcg tcgggttttc gggttgatcg
     8041 gtggcggccc ggcattcgct tcactcggcc ggcaggttca tttccttgag caggtgcggc
     8101 gccggataca ccttggccag cagccagcgc aggtagcgca tgtcgacgtg gatcgcgcgc
     8161 ttgtagcgcg gatcgaacat ccagctggca ctcaccgctt cccagttgct gtcgaagttc
     8221 aggccgatga gcttgccgtt cgcatccagc accggcgagc cggaattgcc gccggtggtg
     8281 tcgaggttgg tcatgaaatt gaccgtctgg gtgcccaggg tgggatccag ggtgctgccg
     8341 aaatcaccct tggcgatggc gtcgatcagc ggcttcggcg catcgaacgg ttccacgccg
     8401 gtgtccttct cgacgatgcc ggcgaccgtg gtcagcgggc ggtagtccat cccgtcgcgc
     8461 ggcgacagcg ggctgacctt gccgtagctg acgcgcaggg tggcgttcgc atccgggtag
     8521 accgccctgc cctgcgcatt gcggtagccc accagcgcct gcatgtaggc cgggcgcagc
     8581 cgcatcagct cgccgttgcg ctgcttggac tcggcctcca gccgcaacag cgcgggctgc
     8641 agggtggcgg cggccttgag cagcgcatct tcggaggccg ccagcgcggc ggcatccagc
     8701 tgcatcgcgg ccaggcgggt ggcctcgtcg cccagtttcg tgccggcgta cagcgcgtcc
     8761 agccgctgtg cggcttcggc ctcgctggcg ccgaacacga cgtcgaattc ggccacgcgc
     8821 aaggcccgcg gcagcgcgaa ataccccttc agcgcgtaac gcagggtcgc cttctccacc
     8881 tcgggatcga aacgccgctg caattgcttc aggccgccct cgatcagcgc ctcgtcgcgc
     8941 tgctggtagc cggattcgcg ttgcacgtcg ggcttgcccc gctccagcga cagccgctgc
     9001 aggctgatcg cggcaccgag caggcccggg cgcaggaacg gcaacagcag atcgcgctcg
     9061 cggttggtct ggaccgcatc cagctgcgcc tgcagcgcgc ggatgtcggc ctgcaccgcg
     9121 acggcatcgg gctgcttcgc cagccacgcc agcatcgccg cttcgtcggc ggcgcgcacg
     9181 cgcaccgcgt cgctgcggcg caggccatcg agttcgccct gcgcgcgctt gagcgtgttc
     9241 ttctgcccgg caacggtgga ggcgtacagc acctgcctgg ccttgtcgcc ggcagtggcg
     9301 gcctcgatcg cctgcagcat gccgcccacc acctcgatcc gcttcggcaa cagccagtcg
     9361 atctggttgg cgaactcgga ggcggtgcgg tggcggaacg tggtccccgg gtaaccggcc
     9421 accatcgcga aatcgcccgc cctcaccggc gcggtggaca cctccaggtg cgcaggcggc
     9481 gcgtacggca cgttgtcctt gctgtacgcc gccggcttgc cgtccttgcc gacgtaggcg
     9541 cggtagaagg tgaagtcgcc ggtgtggcgc ggccacatga agttgtcgac ctcgtcgccg
     9601 tagttgccga tcgcgcgcgg cggcgcatag gccaggcgga tgtccttcaa ctccagttgc
     9661 ttgatcaagt agtactcgcg gccgtagtac atgctggcga cgctgcagcg gtggccggcg
     9721 tcctgctcgc aggccgcgac cgtcgccttc tgcgcggctt gcaccgcgtc gtagtacgca
     9781 cgcccggtct tgccgcgggc ttcggcgagg atgcgatcgg tcacctggtc gaagccgacg
     9841 gtgaccagca cccgcgccgc ggggcccgcg gacggctcat ccgcctgcgt cgccgcgatg
     9901 aagccgtcct ggatcaggtt gcgctccgcg ctggagttca gctggatcgc tcccagcgcg
     9961 cagtggtggt tggtcaccac cagcccctgc ggcgacacga agctggcggt gcagccgccc
    10021 agcgaaacca ccgcgctcat cggatgcctg gtgaggttgg cgaggtcggc cgggttgccc
    10081 ctgaaaccgg cggccttcag ctccttggcc agttgcggca actgggacgg catccacatc
    10141 ccctcgtcgg catgggcggc accaccggcc atggccagcg caagggtcag cagggcggga
    10201 cgcaggcgca ttcggtgttc ctcgaagcag ggcaaacccg cattacagca ccacgcgacc
    10261 ggcgcctgcc tgtgccggcc gtcatggccg cggccgggcc cggggccggc gctagaatgg
    10321 cggcctttcc cgcaaggtgg actgccatgt cgcaggcgac gatgaaggcc ctggtcaagc
    10381 gcgaggcggc caagggcatc tggatggaac aggtgccggt gccgagcccg ggcccgaacg
    10441 aggtcctgat caagctggag aagaccgcga tttgcggcac cgacctgcac atctacctgt
    10501 gggacgagtg gagccagcgc accatcaagc ccgggctggt gatcggccat gaattcgtcg
    10561 gccggatcgt cgggatcggc cccggggtgt ccggctacgc gcctggccag cgggtcagcg
    10621 cggaaggcca catcgtctgc gggcattgcc gcaactgccg cgccggcaag cagcacctgt
    10681 gcccgaacac ggtcggcatc ggcgtgaacc gcaacggcgc cttcgccgaa tacatcgtga
    10741 tgccggccag caacctgtgg ccgatccccg accagatccc cagcgagctg gccgcgttct
    10801 tcgaccccta cggcaatgcc gcccactgcg cgctggagtt cgacgtggtc ggcgaggacg
    10861 tgctgatcac cggcgccggc ccgatcggca tcatcgccgc cggcatctgc aagcacatcg
    10921 gcgcgcgcaa cgtggtggtc accgacgtca acgattaccg cctcaagctg gccgccgaca
    10981 tgggcgccac ccgggtcgtc aacgtcgcca acacctcgct caaggacgtg atgtccgacc
    11041 tgcacatgga gggcttcgac gtcggcctgg aaatgagcgg caacccgcgc gccttcaacg
    11101 acatgctcga ctgcatgtac aacggcggca agatcgcgat gctcgggatc atgccgaagg
    11161 gcgccggggt ggactgggac aagatcatct tcaagggcct gaccctgcag ggcatctatg
    11221 gccggcgcat gtacgagacc tggtacaaga tgacccagtt ggtgctgtcc ggtttcccgc
    11281 tgggcaaggt gctgacccac cagttgccga tcgacgactt ccagaagggc ttcgagctga
    11341 tggaatcggg caaggcgggc aaggtggtgt tgagctggaa ttgacggaag gccgcgatgc
    11401 gcgaggtggt gggggttgcc cgcgcgcgac cttcggcgac atggacgtcg ccgatgagcc
    11461 tacatggacg tattcacggc gtgtcgcgcg cgggcaaccc ccgccgccgc gcgcaaccaa
    11521 ggaacccgca tgtcgctcac cgcacgctac gccgacaccc tcgacgagat ccgcgccgcc
    11581 ggcctgttca agtccgagcg catcatcacc tcgccgcagt ccgccgagat cacccttgcc
    11641 gatggccgca cggtgctgaa cttctgcgcc aacaactacc tgggcctggc cgaccacccg
    11701 gacgtgatcg ccgccgccaa ggacgcgctg gacacccacg gcttcggcat ggccagcgtg
    11761 cgcttcatct gcggcacaca ggacctgcac aagcagctgg aaaagaccat cgccgacttc
    11821 ttcggcaccg aagacaccat cctctacgct gcctgcttcg acgccaacgg cggcctgttc
    11881 gagccgctgc tggacgagaa cgacgcaatc atttccgatg cgctcaacca cgcctcgatc
    11941 atcgacggcg tgcgcctgtg caaggccagg cgctaccgct acgccaactg cgacatggcc
    12001 g
//
LOCUS       DAORUK010000058        11735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_5595_length_11735_cov_17.732962, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000058 DAORUK010000000
VERSION     DAORUK010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 11735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11735
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5595_length_11735_cov_17.732962"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PK818_05515"
     CDS             complement(<1..310)
                     /locus_tag="PK818_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY82227.1"
                     /translation="MDFGFTEEQLMIQDVARRIAREKIAPSAEHHDSTGEFPLENIRT
                     LGENGLMGIEVPAEYGGAGMDPVSYVLAMIEIAEADCAHSTIVSVNNSLFCNGILKFG
                     T"
     gene            538..1467
                     /locus_tag="PK818_05520"
     CDS             538..1467
                     /locus_tag="PK818_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006376775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQY82228.1"
                     /translation="MDLEGWSSRLKVFADATRVRLLALLEGEELTVAELSAITQLAQP
                     RVSTHLAKLKEAGIVRDRRAGVSAYYRFDDAALDPAQRALWQSISAGSDDPLLRQDAE
                     RVPAVLAMRAADQNWADSVAGDMERHYSPGRTWEALARTALPLLAPGDVLDIASGDGV
                     LAELLAPHAGRYVCVDSSQRVVAAAAERLRRFPNVEVREGDMHALPFKDASFDLVVLM
                     HALTYADKPAQAVAEAARVLRKGGRLLLSSLARHEHRSAVEAYGHQNLGFTEKELRRF
                     ADKAGLGIASCETVTREKRPPHFEVISLIGSKP"
     gene            1470..2579
                     /locus_tag="PK818_05525"
     CDS             1470..2579
                     /locus_tag="PK818_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HQY82229.1"
                     /translation="MLPWMHPARVAALEDALARRILVIDGAMGTMIQRHELQEADYRG
                     ERFAEGYDALFAAEGHAHGPGCGCEAHDQRGNNDLLTLTRPDIIRGIHAAYLEAGADL
                     VETNTFNSTSVSLADYRLQHLVRELNREGAALARSACDEAEARDPSKPRFVIGVLGPT
                     SRTASLSPDVNRPGFRATSFDELAEAYRDAARGLVEGGSDVLMVETVFDTLNAKAALF
                     AIDDVFAEIGARLPVMISGTITDASGRTLSGQTAEAFWYSLRHAQPLAIGLNCALGAR
                     DLRQHIDVLAQVADTNVSCHPNAGLPNAFGGYDETPEDMAAVLREFADAGMLNLVGGC
                     CGTSPAHIAAIAGAVAGLPPRAIPRLADAPPELAA"
     gene            2576..5260
                     /gene="metH"
                     /locus_tag="PK818_05530"
     CDS             2576..5260
                     /gene="metH"
                     /locus_tag="PK818_05530"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HQY82230.1"
                     /translation="MTLARNTRLSGLEPLQITPDTNFVNVGERTNVTGSAQFKKLILE
                     GRLDEAVVVARQQVENGAQVIDVNMDEGLLDSAKAMVEYLNLIAAEPDIARIPVMVDS
                     SKWSVIEAGLKCLQGKGIVNSISMKEGEAEFLRQARLVRRYGAAVVVMAFDEAGQADT
                     VARKVGICSRAYKLLTEEIGFPPEDIIFDPNVFAIATGIEEHDGYAVAFIEATRELKR
                     RFPCSHVSGGVSNVSFSFRGNEPVRQAIHVVFLYHAIRAGMDMGIVNAGALPLYDDLD
                     PELRERVEDVVLNRRSDGTERLLEIADRFKGRKGEKKAEDLRWRDRPVRERLGHALVH
                     GIDAYIEVDTEEARLHASRPLDVIEGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKK
                     AVAHLLPYIEAEKLRTGDAGRNNGKIVMATVKGDVHDIGKNIVGVVLACNNFEVIDLG
                     VMVPAQKILDTARAVGADIVGLSGLITPSLEEMSHVAREMQRQGLDMPLLIGGATTSR
                     AHTALKIEPHYKAPTIWVKDASRAVGVAQSLVSAELRGGFVEAAANDYAEIRARHRNR
                     GDARRLAPLHKARAQKFDGGWADYTPPAPRQPGLHVFDDYPLQELLPLIDWTPFFNTW
                     ELAGRYPAILGDAVVGKQARELFADAQAMLARIVAEKWLRAKAVVGLFPAHSVGDDVV
                     ATVDGEAVALHFLRQQVDKPVERPDFCLADFIAPADSGKPDWIGAFAVTAGLGIDEHV
                     ARFEAAHDDYNAILLKALADRLAETLAERMHQRVRTEFWGYAADEALAVDAIIDEAYR
                     GIRPAPGYPACPEHSEKATLFRILDAGANAGMALTESFAMTPASSVSGWYFSHPGSQY
                     FVVGRVSREQVEDYARRKGVGLLQAERWLAANLDYDPE"
     gene            5266..6399
                     /locus_tag="PK818_05535"
     CDS             5266..6399
                     /locus_tag="PK818_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HQY82231.1"
                     /translation="MNGPDGRPPVVAIVGCAGAYGRWLQRFLEARMGLQVIGHDPALA
                     DSEPPLALLQRADVVVFSTPIRDTPAIIGAFATLAGGIEAGQLWLDLTSVKQAPVQAM
                     LASRAEVAGLHPMAAPPKTATLKGHTMAVCRVRVQRWRAWLDALLAALQADCVEIDAQ
                     AHDRAMALVQGLVHAAHMAQAKVLCELAADAGGLPGLHALRTTGHQLDLAVTGRILAG
                     NPAIYTGIQFDNPHVPLVLARLAAAVAELRDAVAEGDDEARAAVTEQFLQVPAAWFGD
                     PVLAARSHDFERMGYLQADLQERCFLSVYLPQDRPGSLRALLDVFERLGVNLASIHSS
                     RTPEGELHFRIGLDLLPVPLAALRAAVEGAGIGRVLDARDDAG"
     gene            complement(6403..7047)
                     /locus_tag="PK818_05540"
     CDS             complement(6403..7047)
                     /locus_tag="PK818_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011408375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 family protein"
                     /protein_id="HQY82232.1"
                     /translation="MSTTLRDQLLGLGFKDAPKPDTPARRRDERSGPQQHKPTPGQRG
                     KPAHATGAPAGKPAHATRPPTGKPAPRTREDIDLAKAYAIRAQREKDERIEAERQKQE
                     EARRRREAKAKLLAFLAGKALNAAEADHVRHFEYGGKIKRIHVTAAQLKALNAGELGV
                     VQQDGRYLLVDAATLAEAGAIFAPAVALKVDPHAPAADDPYADPRYQVPDDLVW"
     gene            complement(7060..7287)
                     /locus_tag="PK818_05545"
     CDS             complement(7060..7287)
                     /locus_tag="PK818_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HQY82233.1"
                     /translation="MSDLESRVVELETRLAFQEQALQELSDALAAARDGETRNALLLH
                     RALEELRQLRSAMAASPITGDAASEPPPPHY"
     gene            complement(7284..8642)
                     /locus_tag="PK818_05550"
     CDS             complement(7284..8642)
                     /locus_tag="PK818_05550"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HQY82234.1"
                     /translation="MRVCIFGTGYVGLVTGTCLAEVGHDVVCVDIDAAKVEGLNRGVI
                     PIYEPGLTPMVQANHAAGRLRFTTDAAAGIAHGDVVFIAVGTPPDEDGSADLTYVLAV
                     ARSIGQHLARPAVVVDKSTVPVGTADKVRAAIASVLAERGADIAFDVVSNPEFLKEGA
                     AVEDCMRPDRIVIGTGSAQALEKLKRLYAPFNRNHERIVAMDVRSAELTKYAANAMLA
                     TKISFMNEIANIAEQVGADVEMVRKGIGSDPRIGWHFIYPGAGYGGSCFPKDVQALAK
                     TAQQHGVQPRLLDAVEAVNAAQKGHLFELIARHYGGVQHLRGKTVAVWGLAFKPNTDD
                     MREASSRTLLAQLWEAGARVRAFDPEAMEETRRIFGDRDGLLLCDSAGEAVQGADALV
                     VVTEWKQFRSPDFGMLKAALADGVVFDGRNLYEPADVEAAGVAYYGIGRGRSLRGDRT
                     GA"
     gene            complement(8721..9506)
                     /locus_tag="PK818_05555"
     CDS             complement(8721..9506)
                     /locus_tag="PK818_05555"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQY82235.1"
                     /translation="MKPVVRATAIAVATMMALGACDKIAEKPAEAAKPAEAAKDAAAS
                     DGKSTYPGLPTEKEQISYTIGMAMGKQLTEIKDEVNLDTVVKAMRSQMAAEKLLLTEE
                     QAKTIFEGFGQKMQAKQIAKMMADAKNNLDNGNKFLAGNGKKDGITTTASGLQYQVLT
                     AGTGAKPGAKDGVKVHYKGTLLDGKTFDSSYDRGEPAVMPLEGVIPGWQEGLQLMAVG
                     GKYKFWIPATLAYGEQAPPMIGPNQVLVFEVELLDIIKPGAKK"
     gene            complement(9559..10485)
                     /locus_tag="PK818_05560"
     CDS             complement(9559..10485)
                     /locus_tag="PK818_05560"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQY82236.1"
                     /translation="MSFPLSARLRAACVLVIALFAGGFAHAQSAPSSERDKVGYMIGM
                     DVAKSVAPAVPDMDMAAFQRAIDNALKGGKPLLDEASSKQVGQALMASINARKGGAAP
                     TAAVDRAKVGLLVGSDIGRSLADIKGEFDMPMFLAGFKAASDPAGKPLLSEADANAVR
                     ASFSARLTADRQAAQIAQAQSNAKAGDDFLARNKAVKGVFTTPSGLQYMVLRQGNGAR
                     PKPGQKVRVNYEGKLLDGTVFDSSYERGQPAEFSLSQVIPGWTEGVGLMPVGGKYRFW
                     IPGKLAYGEKGAGREIPPNSTLTFDVELLGVL"
     gene            complement(10585..11160)
                     /locus_tag="PK818_05565"
     CDS             complement(10585..11160)
                     /locus_tag="PK818_05565"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014647689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HQY82237.1"
                     /translation="MNRARHLLLALVLLLPLVAGAAKGGLLDRSFRPLAGKTPVNLQQ
                     AYGGKVLLVVNTASKCGFTPQFEGLEALHAKYAGKGFAVLGFPSGDFMEQEFTDEKKI
                     QEFCTLTYGVKFPMFAKTHVKGKDADPFYKELAAKTGEAPKWNFHKYLVARDGRTVKA
                     FGSRTAPDDKALVAAIEAALAVPAIPATPAR"
     gene            11291..11554
                     /locus_tag="PK818_05570"
     CDS             11291..11554
                     /locus_tag="PK818_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82238.1"
                     /translation="MNRKLHNTVMAMIASSSLFVLGLLTAGPASPAFAPAPASATLAS
                     TTPADGVASHDRTGKVEATPRRHLRRTHRQSVAMPFFSFAPRG"
     gene            11558..>11735
                     /locus_tag="PK818_05575"
     CDS             11558..>11735
                     /locus_tag="PK818_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQY82239.1"
                     /translation="MTAVEWSDGAPIYRQLKERVVAMLLDGVLKPGDALPSVRQVAAE
                     YQLNPITVSRAYQEL"
BASE COUNT         1742 a         4016 c         4097 g         1880 t
ORIGIN      
        1 cggtgccgaa cttcaggatg ccgttgcaga acagcgaatt gttcaccgac acgatcgtcg
       61 aatgggcgca gtcggcctcg gcgatctcga tcatggccag cacgtagctg accgggtcca
      121 tgccggcgcc gccgtattcg gccgggacct cgatgcccat caggccgttt tcgccgaggg
      181 tgcggatgtt ctccagcggg aactcgccgg tgctgtcgtg gtgttcggcg ctcggcgcga
      241 tcttctcccg cgcgatccgg cgggccacgt cctggatcat caactgctct tcggtgaacc
      301 caaaatccac ggccaactcc gctcgattgc aaaggattgc aattgtagcg ccgggcgtcg
      361 gcgaacccca ccctcgctcg cacgctggcg ggggcggctg gcattcgcca cggggctcga
      421 aatgcccctg cgcgaatacc aggcgctccc gccagcccgc actcccgcgg ctgcgcgact
      481 tgacctgcag gccgcatcga cggacactta tcgccgaatc gcgatggaaa gatatatatg
      541 gatctggaag gctggtcgtc acggctgaag gtgttcgccg atgccacccg cgtgcgcctg
      601 ctggccctgc tcgagggcga ggagctgacc gtggccgaat tgtccgcgat cacccagctg
      661 gcgcagccgc gggtgtccac ccacctggcc aagctcaagg aggccgggat cgtgcgcgac
      721 cgccgcgccg gcgtgtcggc ctattaccgc ttcgacgatg ccgcgctgga cccggcgcag
      781 cgcgccctgt ggcagtcgat cagcgccggc agcgacgacc cgctgctgcg ccaggacgcc
      841 gaacgcgtgc cggctgtgct ggcgatgcgc gcggccgacc agaactgggc ggacagcgtg
      901 gccggcgaca tggagcgcca ctactccccg ggccgcacct gggaggcgct ggcgcgcacc
      961 gccctgccgc tgctggcgcc cggtgacgtg ctggacatcg cctccggcga cggggtgctg
     1021 gccgaactgc tggccccgca cgccgggcgc tatgtctgcg tcgattccag ccagcgggtg
     1081 gtcgcggccg ccgccgagcg cctgcgtcgc ttccccaacg tcgaggtgcg cgaaggcgac
     1141 atgcacgcgt tgccgttcaa ggacgccagc ttcgacctgg tggtgctgat gcacgcgctg
     1201 acgtatgcgg acaagcccgc gcaggccgtc gccgaggcag cgcgggtgct gcgcaagggc
     1261 ggccgcctgc tgctgtccag cctggccagg cacgaacacc gcagcgcggt cgaggcctac
     1321 ggccaccaga acctcggctt caccgagaag gaactgcgcc ggttcgccga caaggccggc
     1381 ctcggcatcg ccagttgcga gaccgtcacc cgcgagaagc ggccaccgca tttcgaggtg
     1441 atctcgctga tcgggagcaa gccttgagca tgttgccgtg gatgcatccg gcgcgcgtgg
     1501 ccgcgctcga agacgcgctg gcacgccgga tcctggtcat cgatggcgcg atgggcacca
     1561 tgatccagcg ccacgaattg caggaagccg actaccgagg tgaacgcttc gccgagggct
     1621 acgacgccct gttcgcggcg gaaggccatg cgcatgggcc gggctgcggc tgcgaggcgc
     1681 acgaccagcg cggcaacaac gacctgctca ccctgacccg cccggacatc atccgtggca
     1741 tccatgcggc ttacctggaa gccggcgccg acctggtcga aaccaacacc ttcaactcga
     1801 ccagcgtatc gctggccgac taccgcctgc agcacctggt gcgcgagctc aaccgcgagg
     1861 gggcggcact ggcgcgcagt gcctgcgatg aggcggaagc gcgcgatccg tcgaagccgc
     1921 gtttcgtgat cggcgtgctg ggcccgacct cgcgcaccgc ttcgctcagc ccggacgtga
     1981 accgccccgg cttccgcgcg acctccttcg acgaactcgc cgaggcctac cgcgacgccg
     2041 cgcgtggcct ggtcgagggc ggcagcgacg tgctgatggt cgagaccgtg ttcgacaccc
     2101 tcaacgccaa ggccgcgctg ttcgcgatcg acgacgtgtt cgcggagatc ggcgcgcgcc
     2161 tgccggtgat gatctccggc accatcaccg atgcctccgg ccgcaccctt tccgggcaga
     2221 ccgcggaggc cttctggtac tcgctgcggc acgcgcagcc gctggcgatc ggcctgaact
     2281 gcgcgctcgg cgccagggac ctgcgccagc acatcgacgt gctggcgcaa gtggccgata
     2341 ccaacgtcag ctgccatccg aacgccggcc tgccgaacgc cttcggcggc tacgacgaaa
     2401 ccccggagga catggcggcg gtgctgcgcg aattcgccga cgccggcatg ttgaacctgg
     2461 tcggcggatg ctgcgggacc tcgcccgcgc acatcgccgc gatcgccggg gcggtggcgg
     2521 ggctgccgcc gcgcgcgatc ccgcggctgg ccgatgcgcc accggagctg gccgcatgac
     2581 gcttgcacgc aacacccgac tcagcggcct ggagcccctg cagatcaccc cggacaccaa
     2641 cttcgtcaat gtcggcgagc gcaccaacgt caccggcagc gcgcagttca agaagctgat
     2701 cctggagggc cggctggacg aggcggtggt ggtcgcccgc cagcaggtcg agaacggcgc
     2761 ccaggtgatc gacgtcaaca tggacgaggg cctgctcgat tccgcgaagg cgatggtcga
     2821 gtacctcaac ctgatcgccg ccgagccgga catcgcgcgc atcccggtga tggtcgactc
     2881 ctcgaagtgg agcgtgatcg aggccgggct gaagtgcctg cagggcaagg gcatcgtcaa
     2941 ctcgatctcg atgaaggaag gcgaggccga gttcctgcgc caggcgcggc tggtgcgccg
     3001 gtacggcgcg gcggtggtgg tgatggcgtt cgacgaggcc ggccaggcgg ataccgtcgc
     3061 gcgcaaggtc gggatctgtt cgcgcgccta caagctgctg accgaagaga tcggcttccc
     3121 gcccgaggac atcatcttcg accccaacgt gttcgcgatc gccaccggca tcgaggagca
     3181 cgacggctac gcggtggcct tcatcgaggc cacgcgcgag ctcaagcggc ggtttccctg
     3241 cagccacgtg tccggcggcg tgtccaacgt cagcttcagc ttccgcggca acgagccggt
     3301 gcggcaggcg atccacgtgg tgttcctgta ccacgcgatc cgcgccggca tggacatggg
     3361 catcgtcaac gccggcgccc tgccgctgta cgacgacctc gatccggaac tgcgcgaacg
     3421 cgtcgaggat gtcgtgctca accgccgcag cgacggcacc gagcgcctgc tggagatcgc
     3481 cgacaggttc aagggcagga agggcgagaa gaaggccgaa gacctgcgct ggcgcgacag
     3541 gcctgtccgc gagcgcctgg ggcatgcgct ggtgcacggc atcgacgcct acatcgaggt
     3601 cgacaccgag gaggcgcgcc tgcacgcatc gcgcccgctg gacgtgatcg aaggcccgct
     3661 gatggcgggc atgaacgtgg tcggcgacct gttcggcgcc ggcaagatgt tcctgccgca
     3721 ggtggtgaag tcggcgcggg tgatgaagaa ggcggttgcg cacctgctgc cttacatcga
     3781 ggcggagaag ctgcgcaccg gcgacgccgg caggaacaac ggcaagatcg tgatggccac
     3841 ggtcaagggc gacgtgcacg acatcggcaa gaacatcgtc ggcgtggtgc tggcctgcaa
     3901 caacttcgag gtgatcgacc tcggggtgat ggtgccggcg cagaagatcc tggacaccgc
     3961 gcgcgcggtc ggcgccgaca tcgtcggcct gtccgggctg atcacgccgt cgctggagga
     4021 aatgagccac gtcgcccggg aaatgcagcg gcaagggctg gacatgccgc tgctgatcgg
     4081 cggcgccacc acttcgcgcg cgcacaccgc gctcaagatc gagccgcact acaaggcgcc
     4141 gacgatctgg gtcaaggacg cgtcgcgcgc agtcggggtg gcgcagtcgc tggtgtcggc
     4201 ggagctgcgc ggcgggttcg tcgaagcggc cgccaacgac tatgcggaga tccgcgcgcg
     4261 ccaccgcaat cgcggcgacg cccgccggct ggcgccgctg cacaaggcgc gcgcgcagaa
     4321 gttcgatggt ggctgggccg attacacgcc accggcgccg aggcagcccg gcctgcacgt
     4381 tttcgacgac tacccgctgc aggaactgct gccgctgatc gactggaccc cgttcttcaa
     4441 cacctgggaa ctggccgggc gctacccggc gatcctcggc gatgccgtgg tcggcaagca
     4501 ggcgcgcgag ctgttcgccg atgcgcaggc catgctcgcg cgcatcgtcg cggagaagtg
     4561 gttgcgtgcg aaggcggtgg tcggcctgtt cccggcgcat tcggtcggcg acgacgtggt
     4621 tgcgacggtc gatggcgaag cggtcgccct gcacttcctg cgccagcagg tcgacaagcc
     4681 ggtcgagcgc ccggatttct gccttgccga cttcatcgcc cccgcggaca gcggcaagcc
     4741 ggactggatc ggtgcgttcg cggtcaccgc gggcctcggc atcgacgaac acgtggcccg
     4801 cttcgaggcc gcccacgacg actacaacgc gatcctgctg aaggcgctgg cggaccgcct
     4861 cgcggagacg ctggcggagc gcatgcacca gcgcgtgcgc accgaattct ggggctatgc
     4921 ggccgacgag gcgcttgcgg tggacgccat catcgacgag gcataccgcg gcatccggcc
     4981 cgcgccaggc tacccggcct gcccggagca cagcgagaag gcgaccctgt tccggatcct
     5041 ggacgccggc gcgaatgcgg ggatggcgct gaccgaaagc ttcgcgatga ccccggcatc
     5101 gtcggtgtcc ggctggtact tcagccatcc gggcagccag tatttcgtgg tcgggcgggt
     5161 gtcgcgggaa caggtcgagg attacgcgcg gcgcaagggc gtgggcctgc tgcaggccga
     5221 acgctggctg gcggcaaacc tggactacga cccggaatga tcgccatcaa tggcccggac
     5281 ggacgcccgc cggtggtcgc catcgtcggc tgcgcgggtg cttacgggcg ctggctgcaa
     5341 cgcttcctgg aagcgcggat ggggctccag gtcatcggcc atgatccggc gctggccgat
     5401 tcggagccgc cgctggcgct gctgcagcgc gccgacgtgg tggtgttcag caccccgatc
     5461 cgcgacaccc cggcgatcat cggcgcattc gccacgcttg ccggcggcat cgaagccggc
     5521 cagctgtggc tggatctcac ctcggtcaag caggcgccgg tgcaggcgat gctggcatcg
     5581 cgggcggagg tggcgggcct gcacccgatg gcggcaccac cgaagacggc gacgctcaag
     5641 ggccacacca tggcggtgtg ccgggtgcgg gtgcagcgct ggcgggcatg gctggatgcg
     5701 ttgctggcgg cactgcaagc cgattgcgtg gagatcgacg cgcaggcgca cgaccgtgcg
     5761 atggcgctgg tgcaggggct ggtgcatgcc gcgcacatgg cgcaggcgaa ggtgctgtgc
     5821 gagctcgcgg cggacgccgg cggactcccg ggcctgcacg cgctgcgcac caccggccac
     5881 cagctcgacc tcgcggtgac cgggcgcatc ctggccggca acccggcgat ctacaccggc
     5941 atccagttcg acaacccgca cgtgccgttg gtgctggcgc ggctggcggc cgcggttgcg
     6001 gagcttcgcg acgcggtcgc cgagggcgac gacgaggccc gtgcggcggt caccgagcaa
     6061 ttcctgcagg tgcccgcggc gtggtttggc gatccggtgc tggccgcgcg cagccacgat
     6121 ttcgagcgca tgggctacct ccaggccgac ctgcaggagc ggtgcttcct cagcgtgtac
     6181 ctgccgcagg accgccccgg ttcgctgcgc gccctgctcg acgtgttcga acggctgggg
     6241 gtgaacctgg cctcgatcca ttcctcgcgc acgccggagg gcgaactgca tttccggatc
     6301 gggttggacc tgctgccggt cccgctggcg gcgctgcgcg cggcggtcga gggcgccggc
     6361 atcgggcgcg tcctggacgc ccgcgacgac gccggctgag gcctaccaga ccaggtcgtc
     6421 ggggacctgg tagcgcgggt ctgcatacgg gtcgtccgcc gccggcgcat gcgggtcgac
     6481 cttcagcgcc accgccggcg cgaagatcgc cccggcctcg gccagggtgg cggcatcgac
     6541 cagcaggtag cgcccgtcct gctgcaccac cccgagttcg ccggcgttga gcgccttcaa
     6601 ctgcgcggcg gtgacgtgga tgcgcttgat cttgccgccg tattcgaagt ggcggacgtg
     6661 gtcggcctcg gctgcgttca gcgccttgcc ggcgaggaac gccagcagct tcgccttcgc
     6721 ctcgcggcgc cggcgcgctt cttcctgctt ctggcgctcg gcctcgatgc gttcgtcttt
     6781 ctcccgctgc gcgcggatcg cgtaggcctt ggccaggtcg atgtcctcgc gcgtgcgcgg
     6841 cgcgggcttg ccggtgggcg ggcgcgtggc atgcgcgggc ttgcctgcgg gcgcaccggt
     6901 ggcatgcgcg ggcttgccgc gctgcccggg cgtgggcttg tgctgctgcg gcccgctgcg
     6961 ttcgtcgcgc cgccgcgccg gcgtgtccgg ctttggggcg tccttgaagc cgaggcccag
     7021 caactggtcg cggagggttg tgctcatgcg ggtcggtggt cagtagtgcg ggggtggcgg
     7081 ttcactggcg gcatcaccgg tgatcgggct ggccgccatc gcgctgcgca gttgccgcag
     7141 ctcctccagc gcacgatgca gcagcaacgc attgcgggtt tcgccgtcgc gggcggccgc
     7201 cagcgcgtcg ctgagctcct gcagggcctg ctcctggaac gcgagccgcg tctccagctc
     7261 gaccacgcgg gattcgaggt cgctcatgcg ccggtccggt cgccacgcag cgagcgcccg
     7321 cgcccgatgc cgtagtaggc cacgccggcg gcttcgacat ccgcgggttc gtacaggttg
     7381 cggccgtcga acaccacgcc gtcggccagc gccgccttga gcatgccgaa gtcggggctg
     7441 cggaactgct tccactcggt gaccaccacc agcgcgtccg cgccctgcac tgcttcgccg
     7501 gcggaatcgc acaacagcag gccgtcgcgg tcgccgaaga tgcgacgggt ctcctccatc
     7561 gcttcgggat cgaaggcgcg cacgcgggca ccggcctccc acagctgggc cagcagggtg
     7621 cggctggacg cctcgcgcat gtcgtcggta ttgggcttga acgccagccc ccacaccgcc
     7681 acggtcttgc cgcgcaggtg ctgcacgccg ccgtaatggc gggcgatcag ttcgaacagg
     7741 tggcccttct gcgcggcgtt gaccgcttcc accgcatcca gcaggcgcgg ctgcacgcca
     7801 tgctgctggg cggtcttcgc cagcgcctgc acgtccttgg ggaaacacga gccgccgtag
     7861 cctgcgcccg ggtagatgaa atgccagccg atgcgcggat ccgacccgat ccccttgcgc
     7921 accatctcca cgtccgcacc gacctgttcg gcgatgttgg cgatctcgtt catgaagctg
     7981 atcttggtcg ccagcatcgc gttggcggcg tacttggtca gttcggccga gcgcacgtcc
     8041 atcgccacga tgcgctcgtg gttgcggttg aacggcgcgt acaggcgctt gagcttctcc
     8101 agcgcctgcg cgctgccggt gccgatgacg atccggtccg ggcgcatgca gtcctcgacc
     8161 gccgcgccct ccttcaggaa ttcggggttg gagaccacgt cgaaagcgat gtccgcgccc
     8221 cgctccgcca gcacggacgc gattgcggcg cgcaccttgt cggcggtgcc caccggcacg
     8281 gtggacttgt ccaccaccac cgccggccgc gcgaggtgct ggccgatgct gcgggcgacc
     8341 gccagcacgt aggtcaggtc ggcgctgccg tcctcgtccg gcggggtgcc gaccgcgatg
     8401 aagacgacat cgccatgggc gatgccggcg gcggcatcgg tggtgaaacg caggcgcccg
     8461 gcggcatggt tggcctgcac catcggggtc aggcccggct cgtagatcgg gatcacgccg
     8521 cggttgaggc cttcgacctt ggccgcgtcg atgtcgacgc agaccacgtc atggccgacc
     8581 tcggccaggc aggtgccggt gaccaggccg acgtaaccgg tgccgaagat gcagacgcgc
     8641 atgcggtggg ttccagggtg gacgtggagc aaagaaacgg ggcgcacccg cgaggatgcg
     8701 ccccgcgggt gtcgcgcaga ttacttcttc gcgcccggct tgatgatgtc cagcagctcg
     8761 acttcgaaca ccagcacctg gttcgggccg atcatcggcg gcgcctgttc gccgtaggcg
     8821 agcgtcgcgg ggatccagaa cttgtacttg ccgccgaccg ccatcagctg caggccttcc
     8881 tgccagcccg ggatcacgcc ttccagcggc atcaccgccg gttcgccgcg gtcatacgaa
     8941 ctgtcgaagg tcttgccgtc cagcagggtg cccttgtagt gcaccttgac gccgtccttc
     9001 gcgcctggct tggcgccggt gccggcggtg aggacctggt actgcaggcc ggaagcggtg
     9061 gtggtgatgc cgtccttctt gccgttgccg gccaggaact tgttgccgtt gtcgaggttg
     9121 ttcttggcat ccgccatcat cttggcgatc tgcttggcct gcatcttctg gccgaagcct
     9181 tcgaagatgg tcttggcctg ctcctcggtc agcagcagct tctccgcggc catctgcgag
     9241 cgcatcgcct tgaccacggt gtcgaggttg acctcgtcct tgatctcggt gagctgcttg
     9301 cccatcgcca tgccgatcgt gtagctgatc tgctccttct cggtgggcag gcccggatag
     9361 gtcgacttgc cgtcgctggc ggcggcgtcc ttggccgctt ccgcgggctt ggcggcttcg
     9421 gcgggctttt cggcgatctt gtcgcaggcg cccagcgcca tcatggtggc gacggcgatg
     9481 gcggtggcac ggacaacggg cttcatcgat tgggactccg gggaatgcga ttgggaatgc
     9541 agcaaggggc gggcgcggtt acaggacgcc gagcagttcg acatcgaagg tcagggtgga
     9601 gttcggcggg atttcccggc ccgcgccctt ctcgccatag gcgagcttgc cggggatcca
     9661 gaaccggtac ttgccgccca ccggcatcag gccaacaccc tcggtccagc ccgggatgac
     9721 ctggctgagg ctgaattcgg ccggctggcc ccgttcgtat gaactgtcga acacggtgcc
     9781 gtcgagcagc ttgccctcat agttgacgcg caccttctgg cccggcttcg ggcgcgcgcc
     9841 attgccctgg cgcaggacca tgtactgcag cccgctcggg gtggtgaaca cgcccttgac
     9901 cgccttgttc ctggcgagga agtcgtcacc ggccttcgcg ttggactggg cctgcgcgat
     9961 ctgcgcggcc tgtcggtccg cggtcaggcg tgccgagaac gaggccctga ccgcgttcgc
    10021 atcggcttcg ctcagcaacg gcttgccggc ggggtcggag gcggccttga acccggccag
    10081 gaacatcggc atgtcgaact cgcccttgat gtcggcaagc gagcgcccga tgtccgaccc
    10141 gaccagcagg ccgaccttgg cgcgatccac cgcggcggtg ggcgcagccc cgcccttgcg
    10201 ggcattgatg ctggccatca gcgcctgccc gacctgcttg ctggaagcct cgtccagcag
    10261 cggcttgccg cctttgaggg cgttgtcgat ggcgcgctgg aacgcggcca tgtccatgtc
    10321 cggcaccgcc ggggccaccg atttcgccac gtccatgccg atcatgtagc cgaccttgtc
    10381 gcgctcgctg gagggggcgg actgggcatg cgcgaaaccg ccggcgaaca gcgcgatcac
    10441 cagcacgcag gcggcgcgca ggcgcgcgct caggggaaag gacatcggga aactcctggg
    10501 gtgtgtgccg ccgtagccta ggtggccatg gctgaacgga ctattgtcgg gtgccgccgc
    10561 ggcgacggca atggcaatga ggcctcagcg ggcgggtgtc gccgggatgg ccgggactgc
    10621 gagcgccgcc tcgatcgccg ccaccagcgc cttgtcgtca ggcgcagtgc gactgccgaa
    10681 ggccttgacg gtccgaccgt cgcgcgccac caggtacttg tggaagttcc acttgggcgc
    10741 ttccccggtc ttggccgcaa gctccttgta gaagggatcg gcgtccttac ccttgacatg
    10801 ggtctttgca aacatcggga acttcacccc gtaggtgagc gtgcagaact cctggatctt
    10861 cttttcgtcg gtgaattcct gctccatgaa gtcgccggac gggaagccca gcaccgcgaa
    10921 gcccttgccc gcgtacttgg cgtgcagggc ctccaggccc tcgaactgcg gggtgaaacc
    10981 gcacttgctg gcggtgttga ccaccagcag caccttgccg ccataggcct gctgcaggtt
    11041 gaccggggtc ttgcccgcga gcgggcggaa actgcggtcc agcaacccgc ccttggccgc
    11101 gccggcaacc agcggcaaca acaggaccag ggcaagcagc aggtggcgtg cgcgattcat
    11161 cgggggctcc gggcattctc ggcgtgggtg ggccaagtat gacttcagtt gggttggtcg
    11221 acacttgacg ggtcgaaaac tagtgttata gttgcctaca acaccgaacc cccagagcag
    11281 gacgttcacc atgaaccgca agctccacaa caccgtcatg gccatgatcg cgagcagcag
    11341 cctgttcgtg cttggcctgc tgaccgcggg cccggccagt ccggcgttcg cgccggcccc
    11401 cgcgtcggcc acccttgcct cgaccacccc ggcggatggc gtggccagcc acgaccgtac
    11461 cggcaaggtc gaggctaccc cccgccggca cctccgccgc acgcaccggc aatcggtcgc
    11521 gatgcccttc ttttcctttg caccccgggg ctgacccatg accgcagtcg aatggagcga
    11581 tggcgctccc atctaccgcc agctgaagga acgcgtcgtc gcgatgttgc tcgacggcgt
    11641 cctcaagccc ggcgacgcgc tgccttcggt acgccaggtg gcggccgaat accagctcaa
    11701 cccgatcacc gtctcgcgcg cctaccagga gctcg
//
LOCUS       DAORUK010000059        11438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_5837_length_11438_cov_18.449091, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000059 DAORUK010000000
VERSION     DAORUK010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 11438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11438
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5837_length_11438_cov_18.449091"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..2247)
                     /gene="glyS"
                     /locus_tag="PK818_05580"
     CDS             complement(175..2247)
                     /gene="glyS"
                     /locus_tag="PK818_05580"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HQY82240.1"
                     /translation="MSMRPLLIELGTEELPVKALPGLAQAFFDGVIAALGKRGVAIER
                     GDAKPLYSPRRLAVLLPGVAGEQPQQRSEVLGPYLTIALDANGAPTRALEGFAAKAGV
                     AWDALEKTTDAKGERFVHRAVTPGAKTADLLQSVLDEALAGMPIPKPMRWGSHAYGFA
                     RPLHWLVALFGDDVVALDALGIRSGRNSRGHRFHHDKHVWIGAPADYIEALRAAHVLV
                     DPDERRARIVAEVETAARAAGGSARIDADNLAQVNCLNEWPVAVACSFERAFLEVPQE
                     ALVATMETNQKFFPVLDAGGRLTEHFIGIANIESHDPSEIRKGYERVIRPRFADAKFF
                     FDEDLKQGLEAMGAGLATVTYQAKLGSVADKAQRVAALAEAIAPQVGVDPALAQRAAL
                     LAKNDLQSRMVNEFPELQGIAGRHYAQCAGEPAEVALAIDEAYQPRFAGDDIALSGIG
                     KVLAIAERLDTLAGGFAAGLKPTGNKDPFALRRNALGLARTIIESGFDLDLKALLAHA
                     NAALASKAVQADAGELYAFILDRLRGYYADKGVPGAHFNAVAELEPASLYDFDRRIDA
                     IGIFAQLPEAAALAAANKRIGNILKKAEGAIPAIEDPALLSEPAERALAEAVEAACAA
                     TGQALAQADYVGTLAHLARLRPQVDAFFDGVMVNVEDPAIRNNRLALLKRLADRLGSV
                     AAIEHLSA"
     gene            complement(2244..3167)
                     /gene="glyQ"
                     /locus_tag="PK818_05585"
     CDS             complement(2244..3167)
                     /gene="glyQ"
                     /locus_tag="PK818_05585"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HQY82241.1"
                     /translation="MAVAITFQQLIQRLNTYWAQQGCVLIQPLDLEVGAGTFHPATFL
                     RALGPEPWNAAYVQPCRRPTDGRYGENPNRLQRYYQYQVAMKPSPDNIVELYFESLKS
                     LGIDPLVHDLRLVEDNWESPTLGAWGLGWEVWLNGMEVTQFTYFQQAGGLECRPVLGE
                     ITYGLERLCMYLQNCDNVYDLVWTYGPDGAPVTYGDVYHQNEVEQSAYNFEHADVAEL
                     FHRFDACEAEAFKLIELGLPLPAYDQVCKASHSFNLLDARRAISVTERQRFILRVRKL
                     SQAVAEAYFAQREKLGFPGLKKSGAKQEVAA"
     gene            3247..3987
                     /locus_tag="PK818_05590"
     CDS             3247..3987
                     /locus_tag="PK818_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HQY82242.1"
                     /translation="MTAPFLILETGQPVAPMRRHGSFAHWIRVAAGLARDEAVVVDVE
                     HGEALPSREGFAGAIVTGSAAMVTDRHDWSERSAHWLRESAHAGLPLLGICYGHQLIA
                     HALGGEVGDNPNGREMGTVCLDLHPPAQQDPLFAGLPARFPAQATHLQSVLRLPQGAT
                     LLARSGQDPAHAFRWGDAVWGLQFHPEFSTTHMRGYIRARSDALAGEGRCGRRLARDV
                     SAAPQARRVLRRFVRHARQHPGAGSRPG"
     gene            4032..4949
                     /locus_tag="PK818_05595"
     CDS             4032..4949
                     /locus_tag="PK818_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82243.1"
                     /translation="MTTRAVGPGAGIEWLKQAFNLGRSNPQAIFGGAALLLATLGAGA
                     IGLSLVLVLLSTALDADPTASLAVSLVVGLGIAMLMAAMMVGYLRLLHAVEQGRPAGA
                     GDVFAGFTDLPASGRAIGFMLLLTVAQYVLVIGLVSMFAHEFGAWYIDSLKASMAGQA
                     AEPMTALPQGFGTAFVLMAAIGLFAYAVQAVGLGQIANGGAGIRGALADGVAGAAKNL
                     LPLLVFLLVLLVAGLVLMLVVGLLAAIVGVIANLVGAWLLLVVGIPLYLGFLLAMFVV
                     MFGVMYFVWRDICGEGEAPAAPADDRIEL"
     gene            complement(4946..5671)
                     /locus_tag="PK818_05600"
     CDS             complement(4946..5671)
                     /locus_tag="PK818_05600"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HQY82244.1"
                     /translation="MPAPAPQARAEAPAAAGFWLRYAAWSLDAACLLPLVALLGMART
                     SRALADARDALQELARELPRLLGEALDAPQPLLDLARRLLLDPAMAAAMARVELSVGA
                     VLATPVLLYALLALPWAVLFEQSRWQATPGKRALGLVVTDAHGRRLKAGHALVRHLAA
                     GLSWLTLNAGHALVLLPPKQLALHDRISDTRVHRANASPRLPWWARAWLALQAIALVA
                     AVAWLYAWLQAAMQAAIEQVLGL"
     gene            complement(5640..6395)
                     /gene="tatC"
                     /locus_tag="PK818_05605"
     CDS             complement(5640..6395)
                     /gene="tatC"
                     /locus_tag="PK818_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017116000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HQY82245.1"
                     /translation="MSEPGLDGDRPLVAHLLELRSRVLRGVAGLVLALLCLLPFANKL
                     YALLAQPLLDKLPKGGQLIATQVASPFFAPMKLAFFVALLVTMPWLLYQAWAFVAPGL
                     YRREKKLALPLLGSALALFYAGCAFAFFLVLPMVFGFLARVTPDGVAMMTDISAYLDF
                     VLVLFLAFGLSFELPVALVILALLGWVTPAQLREGRGYAVVGIFVIAAIVTPPDVVSQ
                     LLLAIPMCLLYEAGIIAASMVSPRASASPASPR"
     gene            complement(6511..6747)
                     /gene="tatB"
                     /locus_tag="PK818_05610"
                     /pseudo
     CDS             complement(6511..6747)
                     /gene="tatB"
                     /locus_tag="PK818_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012481652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
     gene            complement(6763..7002)
                     /gene="tatA"
                     /locus_tag="PK818_05615"
     CDS             complement(6763..7002)
                     /gene="tatA"
                     /locus_tag="PK818_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HQY82246.1"
                     /translation="MGSFSIWHWLIVLVIILLVFGTKKLTGGARDLGKAVDEFKKGMR
                     GDDAKPTARIGDDARPPAEGTSPPENARDRDDAAR"
     gene            complement(7066..7893)
                     /locus_tag="PK818_05620"
     CDS             complement(7066..7893)
                     /locus_tag="PK818_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HQY82247.1"
                     /translation="MRIAPARLALVLAGLLAATAAVAGQREDTRANDAVRVVRQIQSI
                     PESSIPDRLLDEARAIVVVPDTIKAGFMLGGRRGLGLMAVKSPDGTWSNPVFVKLAGA
                     SFGLQAGVQSADVILVFRSERGVDSIVNGKITLGADAGIAAGPVGRNAFAATDGALKA
                     EIWSWSRARGLFAGVALDGAVLSIDNGANRTAYGEGTTPRAVFEGRAPGAPSAAINGF
                     RDALEEATAAARRARAAAAPPANSAPAADGVQVQPLADPNPATTTPLEPVAAPASKP"
     gene            7997..8962
                     /gene="hemH"
                     /locus_tag="PK818_05625"
     CDS             7997..8962
                     /gene="hemH"
                     /locus_tag="PK818_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004088767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HQY82248.1"
                     /translation="MHDSSDAPALLLVNLGTPAAPTAAAVRRYLAQFLHDHRVVQLTR
                     WLWCPLLHFVILPLRSPKVARKYAQVWMDGGSPLAVHTAALADAVQARLPGWRVDHAM
                     RYGSPSMADAFARLRASGVRKVRVLPLYPQYSTTTTASVADAIATDAGGLQVAMLNDY
                     HRDPGWAAAVAGSIRAHWATHGRGERLLLSFHGIPQRLVDGGDPYAAHCEASTLAIAD
                     ALGIPRAEILLTFQSRFGREQWLQPYTQATLEALARDGVRTLDVACPGFAVDCLETLE
                     EIAVENAEAFRAAGGQALRYIPCLNAGAAHADALAGLAQRDQGWA"
     gene            8962..9831
                     /locus_tag="PK818_05630"
     CDS             8962..9831
                     /locus_tag="PK818_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQY82249.1"
                     /translation="MREFEIGIALGRIAGLRNHGEGPRVLALHGWLDNAASFVPLAAH
                     LRGIDLVAIDQPGHGQSAHLPPGTDYSFVGALNAVLDVADALGWDRFALLGHSMGAGI
                     GSMLAAACPQRVERLLAIEALGALAEAPEHTVSRMREAVLAHRGLKDKRLRVFPDVDS
                     AVRTRQYASRVPGSGLDEASVRLLVERGLREVDAGDGTIGYEWSSDPRLTLPTMTRMT
                     EPQVEDLVAGIECPVRAIFADPAQSYLPDALRRRRVALLPQGELVVIAGGHHLHMQQA
                     AEVAAAIGDFFTA"
     gene            complement(9845..10366)
                     /locus_tag="PK818_05635"
     CDS             complement(9845..10366)
                     /locus_tag="PK818_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82250.1"
                     /translation="MTRGLLLAALVAGVVALPGCQRASEAVAEKAFEKAVETASGEKV
                     DIEQDGDTVSIKTDQGEMTVASAQDGGSVALPAGFPDDVFLPAQRTVSSAMDMGGMQA
                     VNVATTATLAAVSADVEKTMQAQGWKREMSMQSGADSSTLIYSKEKRQVVYQLMKADD
                     GGTQLAVRTGNEG"
     gene            complement(10434..11231)
                     /locus_tag="PK818_05640"
     CDS             complement(10434..11231)
                     /locus_tag="PK818_05640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HQY82251.1"
                     /translation="MRSLFRLLSPARIAPKPPALQRDTIAFVLDDGRRIDVSRVRDPR
                     ARRVKLSVDERGVRLTLPARTSLATGERFLHEHRGWLAGQLATQAGHAGDGLQRDATT
                     ALPLRGTLAPLRWQGGRATRLDRDGDGGLLFTAPASAGNAALQRALRDFYEAQGRADV
                     GRWLPKYVAGLPRPPRRIVFKRTATQWGSLAPDATLSLDLSLVLARPSAFEYVLVHEL
                     CHLLHADHSRRFWHAVETRFPHWRDERSYFRAEGRALKARMRTLLTG"
BASE COUNT         1585 a         4001 c         4140 g         1712 t
ORIGIN      
        1 ggcgggcggg gcgtggcggc atcggtgaag gataccgggg cagtgccaaa gcgcgactaa
       61 cccgaaggcg tgccgcctcc ccttgcgtag cagggggagg cccggagggg gttgccgttt
      121 gcgacaaagg gcaccgctgc ccaaccccct tgctttcgca cgggaggggc agggtcaggc
      181 ggacaggtgc tcgatcgcgg ccacgctgcc aagccggtcc gccagccgct tgagcagggc
      241 cagccggttg ttgcggatcg ccggatcctc gacgttgacc atcaccccgt cgaagaacgc
      301 atccacctgc ggccgcagcc gcgccaggtg ggccagggtg ccgacgtagt cggcctgcgc
      361 cagcgcctgc ccggtggcgg cgcaggcggc ttccaccgct tcggccaggg cgcgctcggc
      421 gggttcgctg agcagcgcgg ggtcctcgat ggcggggatc gcaccctccg ccttcttcag
      481 gatgttgccg atgcgcttgt tggccgcggc cagcgcggcg gcttccggca gctgcgcgaa
      541 gatgccgatg gcatcgatgc ggcggtcgaa gtcgtacagc gaggccggtt ccagttcggc
      601 gacggcgttg aagtgcgcgc cgggcacgcc cttgtcggcg tagtagccgc gcaggcggtc
      661 gaggatgaac gcgtagagct cgcccgcatc cgcctggacc gccttcgatg caagcgctgc
      721 attcgcgtgg gcaagcagtg ccttgaggtc gaggtcaaat ccactctcga tgatcgtccg
      781 cgccaacccc agcgcattgc gccgcagcgc gaacgggtcc ttgttgccgg tcggcttgag
      841 gcccgcggcg aaaccgccgg ccagggtgtc caggcgttcg gcgatcgcca gcaccttgcc
      901 gatgccggac agggcgatgt cgtcgccggc gaagcgcggc tggtaggcct cgtcgatcgc
      961 cagcgcgact tcggcgggtt cgcccgcgca ctgcgcgtaa tggcggccgg cgatgccctg
     1021 cagctccggg aattcgttga ccatgcgcga ctgcaggtcg ttcttcgcca gcagtgcggc
     1081 gcgctgcgcg agcgcgggat cgacgccgac ctgcggcgcg atcgcttcgg ccagcgcggc
     1141 cacgcgttgc gccttgtcgg ccacgctgcc cagcttggcc tggtaggtga cggtcgcaag
     1201 gcccgcaccc atcgcttcga gtccctgctt caggtcttcg tcgaagaaga acttggcgtc
     1261 ggcgaagcgc gggcggatca cgcgttcgta gcccttgcgg atttcggacg ggtcgtgcga
     1321 ttcgatgttg gcgatgccga tgaagtgctc ggtcagcctg ccgccggcat ccagcaccgg
     1381 gaagaatttc tggttggtct ccatggtcgc gaccagcgct tcctgcggga cttccaggaa
     1441 cgcgcgctcg aacgagcagg ccaccgccac cggccattcg ttcaggcagt tcacctgcgc
     1501 caggttgtcg gcatcgatgc gggcgctgcc gccggcggcc ctggcggcgg tttcgacttc
     1561 cgcgacgatc cgcgcacggc gttcgtcggg atcgaccagc acatgcgcgg cgcgcagcgc
     1621 ttcgatgtaa tccgccggcg cgccgatcca gacgtgcttg tcgtggtgga agcggtggcc
     1681 gcggctgttg cggccggagc ggatgcccag cgcgtccagc gccaccacgt cgtcgccgaa
     1741 caatgccacc agccagtgca gcgggcgcgc gaatccgtag gcgtggctgc cccagcgcat
     1801 cggcttgggg atcggcatgc cggccagcgc ttcgtcgagc acggactgca gcaggtcggc
     1861 ggtcttcgcg cccggggtga ccgcgcgatg cacgaagcgt tcacccttcg cgtccgtggt
     1921 cttctccagc gcgtcccacg ccaccccggc cttggccgcg aagccttcca gcgcgcgggt
     1981 cggcgcgccg ttcgcatcca gcgcgatggt gaggtacggg cccagcactt cggaccgttg
     2041 ctgcggttgc tcgccggcga cccccggcag cagcaccgcc agccggcgcg gcgagtacag
     2101 cggcttcgca tcgccgcgtt cgatcgccac cccgcgcttg cccagcgcgg cgatgacgcc
     2161 atcgaagaat gcctgcgcca ggcccggcag ggccttgacc gggagttcct cggtaccgag
     2221 ttcgatcagc agcggacgca tgctcatgcc gcaacctcct gcttcgcgcc ggatttcttc
     2281 agtcccggga aaccgagctt ctcgcgctgc gcgaaatacg cctcggcgac cgcctgcgac
     2341 agcttgcgca cgcgcaggat gaagcgctgg cgctcggtga cgctgatcgc gcggcgcgca
     2401 tccagcaggt tgaagctgtg gctggccttg cacacctggt cgtaggccgg cagcggcagg
     2461 cccagttcga tcagcttgaa ggcctcggct tcgcaggcgt cgaagcggtg gaaaagttcg
     2521 gccacgtcgg cgtgctcgaa gttgtaggcg ctctgctcca cttcgttctg gtggtagacg
     2581 tcgccatagg tgaccggcgc gccgtccggc ccgtaggtcc agaccaggtc gtacacgttg
     2641 tcgcagttct gcaggtacat gcacaggcgc tcgaggccgt aggtgatctc gccgagcacc
     2701 ggcctgcatt cgaggccgcc ggcctgctga aagtaggtga actgggtgac ctccatgccg
     2761 ttcaaccaca cttcccagcc caggccccag gcgcccaggg tcgggctttc ccagttgtcc
     2821 tcgaccagcc gcaggtcatg caccagcggg tcgatcccga gcgacttcag cgattcgaaa
     2881 tacagctcga cgatgttgtc cggcgacggc ttcatcgcca cctggtactg gtagtagcgc
     2941 tgcaggcggt tcgggttctc gccgtagcgg ccgtcggtgg ggcgccggca cggctgcacg
     3001 taggccgcgt tccacggctc cgggcccagc gcgcgcagga aggtggccgg atggaaggtg
     3061 ccggcgccga cctccaggtc cagcggctgg atcagcacgc agccctgctg cgcccagtac
     3121 gtgttcaggc gctggatgag ttgctggaac gtgatcgcga cggccatcgg gcttgggatt
     3181 gcggcagggc cggctagtat aagtgcgccg ctttgcccac tccctttcgc gccgcgacga
     3241 cgcccgatga ccgcaccctt cctgatcctc gaaaccggcc agccggttgc cccgatgcgc
     3301 cgccacggca gcttcgccca ctggatccgg gtcgcggccg ggctggcgcg cgacgaggcg
     3361 gtggtggtcg atgtcgagca tggcgaagcg ctgcccagcc gcgagggctt cgccggggcg
     3421 atcgtcaccg gctcggcggc gatggtcacc gaccgccacg actggagcga acgcagcgcg
     3481 cactggctgc gcgagtccgc gcacgccggc ctgccgctgc tcgggatctg ctacggccac
     3541 cagctgatcg cgcatgcgct tggcggtgag gtgggcgaca acccgaacgg ccgcgagatg
     3601 ggcacggtct gcctggacct gcacccgccg gcgcagcagg acccgctgtt cgccgggctg
     3661 ccggcgcgct tcccggcgca ggccacccac ctgcagagcg tgctgcggct gccgcagggc
     3721 gccaccctgc tggccaggtc cgggcaggac ccggcgcatg cgttccgctg gggcgacgcg
     3781 gtctggggtc tgcagttcca cccggagttc agcaccaccc acatgcgtgg ctacatccgc
     3841 gcccgcagcg acgccctggc tggcgaaggc cgctgcggcc ggcggctggc gcgcgacgtc
     3901 agcgccgccc cgcaggcgcg gcgggtgctg cggcggttcg tgcggcatgc acgccagcat
     3961 ccgggggctg gatcgcgccc gggttgagta tgctgggcgc cattggctgg ctggactttg
     4021 gggataccgg aatgacgacg cgtgccgtgg gacctggcgc aggcatcgag tggctgaagc
     4081 aggcgttcaa cctggggcgc agcaacccgc aggcgatctt cggcggtgcc gcgctgctgc
     4141 tggccacgct gggcgccggg gcgatcggcc tgagcctggt gctggtgctg ctgtcgaccg
     4201 cgctggatgc ggatccgacc gcctcgctgg cggtctcgct ggtggtcggg ctggggatcg
     4261 cgatgctgat ggcggcgatg atggtgggct acctgcgcct gctgcatgcg gtcgagcagg
     4321 gccggccggc cggcgccggc gacgtgttcg ccggcttcac cgacctgccc gccagcggcc
     4381 gcgcgatcgg cttcatgctg ctgctgacgg ttgcccagta cgtgctggtg attggcctgg
     4441 tcagcatgtt cgcgcacgaa ttcggcgcct ggtacatcga cagcctgaag gcctcgatgg
     4501 ccgggcaggc ggcggaaccg atgaccgcgc tgccgcaggg cttcggcacc gcgttcgtgc
     4561 tgatggcggc gatcgggctg ttcgcctacg cggtgcaggc ggttggcctg ggccagatcg
     4621 cgaacggtgg cgcgggcatc cgtggcgcgc tcgccgacgg cgtggccggc gcggcgaaga
     4681 acctgttgcc gctgctggtg ttcctgctgg tactgctggt ggcgggcctg gtgctgatgc
     4741 tggtggtggg cctgctggcg gcgatcgtcg gcgtgatcgc caacctggtt ggcgcctggc
     4801 tgctgctggt ggtcgggatc ccgctctacc tcgggttcct gctggcgatg ttcgtggtga
     4861 tgttcggggt gatgtatttc gtgtggcgcg acatctgcgg cgagggcgag gcaccggccg
     4921 cgccggccga cgaccggatc gaactctaga ggcccagcac ctgctcgatc gccgcctgca
     4981 tcgcggcctg caaccaggcg tacagccagg ccacggcggc gaccagcgcg atcgcctgca
     5041 gcgccagcca tgcgcgggcc caccacggca ggcgcggcga agcgttggcg cggtgcacgc
     5101 gggtatcgct gatgcggtcg tgcagcgcca gctgcttcgg cggcagcaac accaacgcat
     5161 gcccggcatt gagcgtgagc caggacaggc cggcggcgag gtggcgcacc agcgcatgcc
     5221 cggccttcag gcggcggccg tgcgcatccg tcaccaccag cccgagcgcg cgcttgcccg
     5281 gcgtggcctg ccaccgcgac tgttcgaaca gcaccgccca cggcagcgcc agcagcgcat
     5341 acagcaacac cggcgtggcc aggaccgcgc ccaccgacag ctccacccgg gccatggcag
     5401 cggccatcgc cggatccagc agcaggcgcc gggccagatc gagcagcggt tgcggcgcgt
     5461 ccagcgcctc gcccagcagg cgcggcagtt cgcgggcgag ctcctgcagc gcatcgcgcg
     5521 catcggccag cgcccggctg gtcctcgcca tgccgagcag cgccaccagt ggcaacaggc
     5581 aggccgcatc cagcgaccag gccgcgtagc gcagccagaa accggccgcc gccggcgcct
     5641 cagcgcgggc ttgcggggct ggcgctggca cgcggcgaca ccatcgaggc ggcgatgatc
     5701 ccggcctcgt acagcaggca catcgggatc gccagcagca gctggctgac cacgtcgggt
     5761 ggggtcacga tcgcggcgat cacgaagatc ccgaccaccg cgtaaccacg gccttcgcgc
     5821 agctgcgccg gggtcaccca gccaagcagc gccaggatca ccagcgccac cggcagctcg
     5881 aagctcaggc cgaaggccag gaacagcacc agcacgaaat ccaggtaggc gctgatgtcg
     5941 gtcatcatcg ccaccccgtc cggggtcacc cgggcgagga agccgaacac catcggcagc
     6001 accaggaaga aggcgaacgc gcagccggcg tagaacagcg ccagcgccga tcccagcagc
     6061 ggcagcgcga gtttcttctc gcgccggtac aggcccggcg ccacgaaggc ccaggcctgg
     6121 tacagcaacc acggcatcgt caccagcagg gccacgaaga acgccagctt catcggcgcg
     6181 aagaacggcg aggccacctg ggtggcgatc agctggccgc ccttcggcag cttgtccagc
     6241 agcggttgcg ccagcagcgc atacagcttg ttggcgaacg gcagcaggca cagcagcgcc
     6301 agcaccagcc cggcgacccc gcgcagcacc cgcgaacgca gctccagcag gtgcgccacc
     6361 aacgggcgat cgccgtcgag gcccggctca gacatcgcgc ggctcgcggg ccgatgcggc
     6421 cgcgtgcacg ggttcggcaa cggccacggc gtccgatccg gcttctgctt cggcttcggc
     6481 ttcgagcgat tcgcgggtgt cggcggccag cgcgtgcagc tcgcgttcgc tgtcctccag
     6541 cgcctgcctg ccgtcctgca ggctgcgccg gagttcctcg ctggccagtt cgctttccag
     6601 ctcggccttc accgaatacc agtgcgcgcg cgccttgcgt acccacagcc cggtgaagcg
     6661 cgcggccttg ggcaggcgct ccggccccag caccaccagc gcgaccaggg cgaccagcag
     6721 catttcgccg aaggagaaat cgaacatcgg cgcgggcgcg gctcagcgcg ccgcgtcgtc
     6781 gcggtcgcgc gcgttctcgg gcggggaagt gccttccgcc ggcggcctgg cgtcgtcgcc
     6841 gatgcgtgcg gtgggcttgg catcgtcgcc gcgcatgccc ttcttgaact cgtcgaccgc
     6901 cttgccgagg tcgcgggcgc caccggtgag cttcttggtg ccgaagacca gcaggatgat
     6961 caccagcacg atcaaccagt gccagatgct gaagctgccc atggatgcac cgaaagatgg
     7021 aggaattgcg ccaattctag cgccgatcca ccggcgcccc gtggctcagg gcttgctggc
     7081 gggtgccgcc accggctcca gcggggtggt ggtggcgggg ttggggtcgg ccagcggctg
     7141 cacctgcacg ccgtccgccg cgggtgcgct gttcgccggc ggcgcggcgg cggcgcgtgc
     7201 gcgccgggcg gccgcggtgg cctcttcgag tgcatcgcgg aagccgttga tcgcggccga
     7261 gggtgcgccc ggggcgcggc cttcgaacac cgcccgcggg gtggtgccct cgccataggc
     7321 ggtgcggttg gcgccgttgt cgatgctgag cacggcgccg tccagcgcca ccccggcgaa
     7381 cagtccgcgc gcgcgcgacc acgaccagat ctccgccttc aacgcgccat cggtggcggc
     7441 gaaggcattg cggccgaccg gtcccgcggc gatgcccgca tccgcgccaa gggtgatctt
     7501 gccgttgacg atggaatcca ccccgcgttc gctgcggaac accaggatca cgtcggcgga
     7561 ctgcacgccc gcctgcaggc cgaagctggc gcccgcgagc ttgacgaaca ccgggttcga
     7621 ccaggtgcca tcgggcgatt tcaccgccat caggccaagc ccgcggcggc cgccgagcat
     7681 gaagccggcc ttgatcgtgt cggggaccac cacgatcgcc ctggcttcat ccagcaggcg
     7741 gtccgggatc gaggattccg ggatcgactg gatctgccgc accacccgca ccgcgtcgtt
     7801 ggcgcgcgtg tcctcgcgct ggccggcaac ggcggcggtg gcggcgagca atccggccag
     7861 gaccagggcg aggcgggcgg gggcgatgcg catggggatc tccggtcagg ggcggcaggt
     7921 tgccatcgtg gcgatgcggc gatgaatcga tcttaagtcg cgtcgccggc ttcgcgaccc
     7981 gtgcgatcct atgccaatgc acgactcctc cgatgcgccg gcgctgctgc tggtcaacct
     8041 gggcacgccg gcggcaccca ccgccgcggc ggtgcgccgc tatctcgcgc agttcctgca
     8101 cgaccaccgg gtggtgcaac tcacccgctg gctgtggtgc ccgttgctgc acttcgtgat
     8161 cctgccgctg cgcagcccga aggtggcgcg caagtatgcg caggtctgga tggacggcgg
     8221 ctcgccgctg gcggtgcaca ccgccgcgct ggcggatgca gtgcaggcgc gcttgccggg
     8281 ctggcgggtg gaccacgcga tgcgctacgg ctcgccgtcg atggcggacg ccttcgcgcg
     8341 cctgcgcgcg tccggcgtgc gcaaggtccg ggtgctgccg ctgtatccgc agtattcgac
     8401 caccaccacc gccagcgtcg ccgatgccat cgccaccgat gccggcggcc tgcaggtggc
     8461 gatgctcaac gactaccacc gtgatccggg ctgggccgcc gcggtggccg ggtcgatccg
     8521 cgcgcactgg gccacgcacg gccgcggcga gcgcctgctg ctgtcgttcc acggcatccc
     8581 gcagcgcctg gtcgatggcg gcgatccgta cgcggcgcac tgcgaggcca gcacgctggc
     8641 gatcgccgac gcactgggca tcccgcgcgc ggagatcctg ctgaccttcc agtcgcgctt
     8701 tggccgcgag caatggctgc agccgtatac ccaggccacc ctggaggcgc tggcgcgcga
     8761 cggcgtgcgc accctcgacg tggcctgccc ggggttcgcg gtggactgcc tggaaacgct
     8821 ggaggaaatc gcggtggaga atgcagaggc cttccgcgcg gccggcggcc aggccttgcg
     8881 ctacatcccc tgcctcaacg ccggcgccgc gcatgccgac gcgctggcgg gcctggcgca
     8941 acgggaccag ggctgggcct gatgcgcgaa ttcgagatcg ggattgcgct cggccgcatc
     9001 gccggattgc gcaaccatgg cgagggcccg cgcgtgctgg cgctgcatgg ctggctcgac
     9061 aatgccgcca gcttcgtgcc gctggcggcg cacctgcgcg ggatcgacct ggtcgcgatc
     9121 gaccagcccg gccacgggca aagcgcgcac ctgccgccgg gtaccgacta ttccttcgtc
     9181 ggcgcgctca atgcggtgct ggacgtcgcc gatgcgctgg gctgggaccg cttcgccctg
     9241 ctcgggcatt cgatgggtgc cgggatcggc agcatgctcg ccgccgcctg cccgcagcgg
     9301 gtcgaacggc tgctggcgat cgaggcgctc ggcgcgctgg cggaagcccc ggagcacacg
     9361 gtgtcgcgca tgcgcgaggc ggtactcgcc catcgcgggc tgaaggacaa gcggctgcgg
     9421 gtgtttcccg acgtcgacag cgcggtgcgc acccgccagt acgccagccg ggtgcccggc
     9481 agcgggctgg acgaggcgtc ggtacggctg ctggtcgaac gcggcctgcg cgaagtcgac
     9541 gccggcgacg gcaccatcgg ctacgaatgg agcagcgacc cgcgcctgac cctgccgacg
     9601 atgacgcgga tgaccgagcc gcaggtcgag gacctggtgg ccgggatcga atgcccggtg
     9661 cgggcgatct tcgccgaccc ggcgcagtcc tacctgccgg acgcgctgcg ccggcggcgg
     9721 gtggcgctgc tgccgcaggg tgagctggtg gtgatcgccg gcggccacca cctgcacatg
     9781 cagcaggcgg ccgaggtcgc cgccgcgatc ggcgatttct tcaccgcctg agccggcgcg
     9841 ggggtcagcc ctcgttgccg gtgcgcaccg cgagctgggt gccgccgtcg tcggccttca
     9901 tcagctggta caccacctgg cgcttttcct tgctgtagat cagggtcgag ctgtcggcgc
     9961 cggactgcat cgacatctcg cgcttccagc cctgcgcctg catggtcttc tcgacgtcgg
    10021 ccgagaccgc ggccagggtg gcggtggtgg cgacgttgac cgcctgcatg ccgcccatgt
    10081 ccatcgcgct gctgaccgtg cgctgcgccg gcaggaacac gtcgtccggg aagccggcgg
    10141 gcagggcgac gctgccgccg tcctgcgcgg aggcgaccgt catctcgccc tgatcggtct
    10201 tgatgctgac ggtgtcgccg tcctgctcga tgtcgacctt ttcgccgctg gcggtctcga
    10261 ccgctttctc gaacgccttc tcggcgaccg cttcgctggc gcgctggcag cccggcaagg
    10321 cgaccacgcc ggcgaccagc gcggcaagca gcagtccccg cgtcatagga agttgtggca
    10381 ttgcattgtt cctggaatcc cctgggtaac ccgggccaac gcaatgcggg gactcaaccg
    10441 gtcagcagcg tgcgcatgcg cgccttcagc gcccgcccct cggcgcggaa atacgaccgc
    10501 tcgtcgcgcc agtgcgggaa gcgcgtttcc actgcgtgcc agaaccggcg cgagtggtcg
    10561 gcatgcagca ggtggcagag ttcgtgcacc agcacgtatt cgaaggcgga cggacgcgcc
    10621 agcaccagcg acaggtccag cgacagggtc gcgtccggcg ccagcgagcc ccactgggtg
    10681 gcggtgcgct tgaacacgat gcggcgcggc gggcgcggca ggccggcgac gtacttcggc
    10741 agccagcggc cgacatcggc gcggccctgg gcttcgtaga aatcgcgcag tgcgcgttgc
    10801 agggcggcat tgccggcgga ggcgggcgcg gtgaacagca ggccgccgtc gccatcgcgg
    10861 tcgagccggg ttgcgcgccc gccctgccag cgcagcggcg ccagcgtgcc ccgcagcggc
    10921 agcgccgtgg tggcatcccg ctgcaggccg tcaccggcgt gtccggcctg ggtcgccagc
    10981 tgcccggcga gccagccgcg gtgctcgtgc aggaagcgtt cgccggtggc caggctggtc
    11041 cgggccggca gggtcaggcg caccccgcgt tcgtcgacgc tcagcttgac ccgccgcgcg
    11101 cgcgggtcgc gcacgcgcga cacgtcgatg cggcggccgt cgtcgagcac gaaggcgatg
    11161 gtgtcgcgct gcagcgccgg cggtttcggc gcgatgcggg cgggcgagag caggcggaac
    11221 agggaacgca tgcgctgagt gtaagggctc gcttcgcggg gcgaacggcg cgccgggcgc
    11281 gcattcgctg cgcggctcga aatgccgctg cgcgaacgcc cgccccgcac gccgtccgcc
    11341 gcgcggactt gcgctactcg gccttggtga gcttgaacgc cttttccagc acgttgaaca
    11401 ggcgcttgaa ctcgccggcc atcagcgcga agcgggcg
//
LOCUS       DAORUK010000060        11100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_6097_length_11100_cov_18.358533, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000060 DAORUK010000000
VERSION     DAORUK010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 11100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11100
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6097_length_11100_cov_18.358533"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /gene="cysK"
                     /locus_tag="PK818_05645"
     CDS             <1..833
                     /gene="cysK"
                     /locus_tag="PK818_05645"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020851740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HQY82252.1"
                     /translation="DRLALAIVLDAEQRGLLKPGDTIIEATSGNTGVALAMVAAARGY
                     KFVATMTETFSIERRKLMRAYGAKVILTPAAERGSGMVRRAKELADAHGWFLASQFAN
                     PANPAYHRQTTAPEILRDFAGKRLDYFVSGWGTGGTLTGVGEVLKVARPQTRIVATEP
                     AGASLLGGAEWKPHKIQGWTPDFVPEVLNREVHDELHQVTDEDAIATARRLAAEEGIF
                     VGISAGGTLATALKVADTAPEGAVLLAMLPDTGERYFSTPLFAGVNEGSDDEWLASLG
                     "
     gene            complement(1045..2448)
                     /locus_tag="PK818_05650"
     CDS             complement(1045..2448)
                     /locus_tag="PK818_05650"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HQY82253.1"
                     /translation="MTKLQPVLLSGGSGTRLWPLSREAYPKQFLALAGDDTMLQATWQ
                     RVAAIADAAPIVVAGEEHRFLVAEQLRQIGAPTPAILLEPVGRNTAPAIAAAAMQALR
                     EGDDPLLLVLPSDHVVRDAAAFRAAVRGAMPVAEAGALVTFGIVPDAPETGFGYIEAA
                     PGDGVRKVLRFVEKPDAATAQGYLAAGGYYWNSGMFLLRASRYLEELAKFRPDINAGT
                     RAAFDLAQRDGDFIRLDKGAFAACPADSIDYAVMENTDAAMVLPVEIGWNDVGSWSAL
                     WEVSAQDAAGNAHHGDVIAIDSRNSYAYARRLVALVGVDDLVVVETDDAVLVARKDRV
                     QQVKDVVARLKSDGRTQAALHREVHRPWGSYDSIDMAEGFQVKRIKVRPGARLSLQSH
                     TRRAEHWIVVRGTARVTRDNDVFELFANQSTYIPIGAKHRLENPGAEMLELIEVQSGD
                     YLGEDDIVRYEDVYGRS"
     gene            2491..3525
                     /locus_tag="PK818_05655"
     CDS             2491..3525
                     /locus_tag="PK818_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQY82254.1"
                     /translation="MSQPEPSDPRAEARTAWTRAILRDPGAIPVRASMDAGFRSYWRV
                     AGADGSRIVMDSPPDKEDVRPWLRIRDVLEAGGVRVPQVLARDVDAGFLLLEDLGADT
                     YLHVIDATNADALFDAAVSQLLKLQAIAPPADLPAYDEALLARELRLFDEWFCGTHLG
                     LSLQCAEVDVLDHAYRVLVDAALAQPQVLVHRDFMPRNLMPINSGPATDGPAVLDFQD
                     AVRGPIAYDALSLFKDAFLSWPEDRVDAWLDRYHARAVAAGLPVPALARFRRDADLIG
                     VQRHLKVIGIFARLHHRDGKPKYLADVPRFFGYLDQALPKYPELAGLHGFIEQRVKPA
                     MAQRAGGDRA"
     gene            3522..4238
                     /locus_tag="PK818_05660"
     CDS             3522..4238
                     /locus_tag="PK818_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011348135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQY82255.1"
                     /translation="MKALIFAAGLGERMRPLTDTTPKPLLAVGGKPLVEWHLEKLAAC
                     GVEDVVINTSWLAQQFPATLGDGARWGLRIRYSYEGATPLETGGGMWNALPLLGDAHG
                     SNEAFLLVNGDVWTDFDFARLPREPQGLAHLVMVDRPPQATHGDFSLAADGVIRADGE
                     HRLTYSGVGVYRPRLFDGWRAIVPEPAIVDGKPKFPLAPILRAHMGEGMVRGEHHRGR
                     WTDVGTPQRLAQLDAELAAR"
     gene            complement(4235..4939)
                     /gene="hda"
                     /locus_tag="PK818_05665"
     CDS             complement(4235..4939)
                     /gene="hda"
                     /locus_tag="PK818_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007970599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HQY82256.1"
                     /translation="MVAQDRAGPQLPLALRHSPDQRFEGWVGAPAVLAQLQATAAGES
                     SDSLYLQGGSGSGKTHLLLAACAAAEAAGRHANYLSLARVRGHARDALQGIEQAGLVA
                     LDDLDALAGNRDDEIALFDLHNRVRDAGVALAYAARALPGALPLQLPDLRSRLAHCTL
                     LALRALDDAGRAQVLRQRAASRGLQFDEAALEWLLTRCSRDLSDLAALFERLDHAALA
                     AQRRLTVPFLRQVLGA"
     gene            complement(5026..6108)
                     /locus_tag="PK818_05670"
                     /pseudo
     CDS             complement(5026..6108)
                     /locus_tag="PK818_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
     gene            complement(6115..7239)
                     /locus_tag="PK818_05675"
     CDS             complement(6115..7239)
                     /locus_tag="PK818_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HQY82257.1"
                     /translation="MQRKSWATRAASHMLAVLLLLCAGIAHAQRVEGDRAGASGPYEA
                     EVAVRNQGDAERNAGFGRALAAVLAKVTGDRGAAQRPGVREELGKAGDYVAGYDYRQD
                     EGVSSSGAPSFQTTLVARFKPDDVDQLVQMLGLPAWPLPRPKPVLWLAINDGSGPRLV
                     GLGQVNAARAILDQAKARGFALGLPGGNAAEQAAVGAIWRGDTGAIAGLSRKYSPPMQ
                     LIGKLQRGTGGWVADWTLVDKGKTLSSWQTKHADARRAMVGGADGAADALFKRYARAG
                     SGGPSGTYRVRILGVDSADDYMRLAGYLDDLSIVRQVRPVLALPGELQLDLELATGIS
                     SFARLVDRGGVLSTISTGGGPGKDGDPATAPGVASFRLGG"
     gene            7392..8456
                     /gene="purM"
                     /locus_tag="PK818_05680"
     CDS             7392..8456
                     /gene="purM"
                     /locus_tag="PK818_05680"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HQY82258.1"
                     /translation="MTQSTPPAAAGLTYRDAGVDIDAGNEVVERIKPLVKRTLRPEVL
                     GGVGLFGGLFDLSSRYREPVLVSGTDGVGTKLILAKQLNRHATIGQDLVAMCVNDVLV
                     QGAEPLFFLDYFATGKLDVDTTVHVVGGIARGCEIAGCALIGGETAEMPDMYPPGEYD
                     LAGFTVGAVEKSKLVDSGKLRAGDVLIGIASSGPHSNGYSLVRKILARAGITPDNGGL
                     DLDLGGVTLADALMEPTRIYVKPVLELLARHDIHAMAHVTGGALVEKIIRVVPAPLGL
                     DIDTSSWPLPAVFDWLQREGNVAREEMWRTFNCGIGYVLMADPADVAAIEADLDRLQL
                     AHWRIGQVTPPRDGERLRIR"
     gene            8464..9102
                     /gene="purN"
                     /locus_tag="PK818_05685"
     CDS             8464..9102
                     /gene="purN"
                     /locus_tag="PK818_05685"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014502504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HQY82259.1"
                     /translation="MRLAVLASGRGSNLQAILDAIAAGTLDAEIVGVFSDKPTAQALR
                     RVPEPLRWSRNAKDYPNRAAFDAELATAVAATRPDWVVCAGYMRILGDAFVQRFRGHL
                     LNIHPSLLPRHRGLRTHAGALAAGDGEHGASVHFVVPELDAGAVIAQAALPVLATDTP
                     ESLAARVLRLEHPLLVAVLQMAAAGRITEQAAQATLDGQPLFTPLRLEFAPG"
     gene            9146..9820
                     /locus_tag="PK818_05690"
     CDS             9146..9820
                     /locus_tag="PK818_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006453199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HQY82260.1"
                     /translation="MKSVRVAALLSVLLAASPAWAVKPFTADYEASVMGGISADAQMT
                     LASAGGDRWNYVLSVDSPVATLRQATVFEDRDGAWRPLSGNDSTQMLIKKSQKNASYD
                     WARGEARWSGDVKADRAGPVKLQEGDLDAMLLNLAIVRDVAAGKPLTYRMVDNGVVRT
                     QAYQNLGKDTVTYAGKPRQATKVSRSSENKQVIVWVVDGLPVPARILQKKDGKDELDL
                     VLQSVR"
     gene            complement(9916..>11100)
                     /gene="murA"
                     /locus_tag="PK818_05695"
     CDS             complement(9916..>11100)
                     /gene="murA"
                     /locus_tag="PK818_05695"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQY82261.1"
                     /translation="CATLLADAPVRITNVPRLHDVFTTAKLLAGLGAGIEHRGDVMTV
                     DPRSVNSHVAPYELVKTMRASVLVLGPLLAKHGDAEVSLPGGCAIGSRPVDLHIKGLQ
                     ALGAEITVDHGFIKARSAGRLKGARHVFEMVSVGATENVLMAAALADGTTVLENAAME
                     PEIVDLAECLVAMGAKITGAGTHKITIEGVERLSGCEHAVVADRIETGTFLVAAAMTG
                     GRVTATHARADTLDAVLDKLREAGAEISIESGTGDGDRILLDMHGKRPRAVNLTTAPH
                     PAFPTDMQAQFMALDCIADGVGVINETIFENRFMHVNELLRLGADIRVDGHTAVVRGV
                     AQLSGAPVMATDLRASASLILAGLVADGDTVIDRIYHLDRGYENIEAKLSALGASIRR
                     VD"
BASE COUNT         1701 a         4055 c         3787 g         1557 t
ORIGIN      
        1 aggaccggct ggcgcttgcg atcgtcctcg acgccgagca gcgcgggctg ctgaaaccgg
       61 gcgacacgat catcgaggcg acctccggca acaccggcgt ggcgctggcg atggtcgccg
      121 ccgcgcgcgg ctacaagttc gtggcgacca tgacggagac cttttcgatc gaacgccgca
      181 agctgatgcg cgcgtatggc gccaaggtga tcctgacccc ggccgccgaa cgcggcagcg
      241 gcatggtgcg ccgggcgaag gaactggcgg acgcacacgg ctggttcctg gccagccagt
      301 tcgccaaccc ggccaatccc gcctaccacc gccagaccac cgcgccggaa atcctgcgcg
      361 acttcgccgg caagcggctg gactacttcg tcagcggctg gggcaccggc ggcaccctga
      421 ccggggtcgg cgaggtgctg aaggtcgcgc gcccgcagac ccggatcgtg gccaccgaac
      481 cggccggcgc gtccctgctc ggcggtgccg aatggaagcc gcacaagatc cagggctgga
      541 ccccggattt cgtgccggag gtgctcaacc gcgaggtgca tgacgaactg caccaggtca
      601 ccgacgaaga cgccatcgcc accgcgcgcc ggctggccgc cgaggaaggc atcttcgtcg
      661 gcatttccgc cggcggcacc ctcgcgaccg cgctgaaggt ggccgacacc gcgcccgaag
      721 gcgctgtgct gctggcgatg ctgccggata ccggcgagcg ctatttctcc acgccgctgt
      781 tcgccggcgt gaacgagggc agcgacgacg agtggctggc cagcctgggt tgagccgggc
      841 gcgatccgca ccacgcacaa gcccatccgc gctgcggacg gggttttcca ttccaggcgc
      901 cacgatgccc caggccgcct tgccgcgggc actgcctgca gcgaaatcaa ggaggaggca
      961 ttggccgctg gccaatgccg ggggacattc gcggaaggca gtgcccgcgg gaaggcggcc
     1021 acgagagcgt ggcagcgggc aatctcacga ccgcccgtac acgtcctcgt agcggacgat
     1081 gtcgtcctcg cccaggtaat cgccggactg cacctcgatc agttccagca tctccgcacc
     1141 cggattctcg aggcggtgct tcgcgccgat cgggatatag gtgctctggt tggcgaacag
     1201 ctcgaacacg tcgttgtcgc gcgtgacccg cgcggtgccg cgcaccacga tccagtgctc
     1261 ggcgcggcgc gtgtgcgact gcaacgacag ccgcgcaccc ggcctgacct tgatccgctt
     1321 gacctggaag ccctcggcca tgtcgatgga atcgtagctg ccccacggcc tgtgcacctc
     1381 gcgatgcagc gcggcctggg tgcgcccgtc cgacttcagc ctggccacca cgtccttgac
     1441 ctgctgcacg cggtccttgc gcgcgaccag caccgcgtcg tcggtttcga ccaccaccag
     1501 gtcatcgacc ccgaccagcg ccaccagccg ccgcgcgtag gcatagctgt tgcggctgtc
     1561 gatcgcgatc acgtcaccgt ggtgggcgtt gccggcggca tcctgcgcgc tcacctccca
     1621 cagcgccgac cagctgccga cgtcgttcca gccgatctcg accggcagca ccatcgccgc
     1681 atcggtgttc tccatcaccg cgtagtcgat ggagtccgcc gggcacgccg cgaacgcgcc
     1741 cttgtcgagg cggatgaagt cgccgtcgcg ctgggcaagg tcgaatgcgg cccgcgtgcc
     1801 ggcgttgatg tccggtcgga acttcgccaa ctcctccagg tagcgcgagg cgcgcagcag
     1861 gaacatgccg ctgttccagt agtaaccacc ggcagcgagg tagccctgcg cggtggccgc
     1921 gtcgggcttc tcgacgaagc gcagcacctt gcgcacgccg tcgcccggcg cggcctcgat
     1981 gtagccgaag ccggtctccg gcgcatccgg cacgatgccg aaggtgacca gcgcacccgc
     2041 ttcggccacc ggcatcgccc cccgcaccgc cgcccggaag gccgcggcat cgcggaccac
     2101 gtggtccgag ggcagcacca gcaacagcgg gtcgtcgccc tcgcgcagcg cctgcatggc
     2161 ggcggcggcg atcgccggcg cggtgttgcg gccaaccggc tccagcagga tcgcgggcgt
     2221 cggcgcaccg atctggcgca actgttcggc gaccaggaag cgatgctcct cgcccgccac
     2281 cacgatcggc gcggcatcgg cgatcgcggc cacccgttgc caggtcgcct gcagcatggt
     2341 gtcgtcgccg gccagggcca ggaactgctt cggataggcc tcgcgcgaga gcggccacag
     2401 gcgcgtcccg gaaccgccgg acaacagcac gggctgcagc ttggtcatgc gcgtctcgct
     2461 cggatcgatg ccgcagttta ccctgtgcac atgagccaac ccgagccttc cgacccgcgc
     2521 gccgaggcgc gcaccgcatg gacgcgcgcg atcctgcgcg atcccggcgc catccccgtg
     2581 cgtgcctcga tggatgccgg cttccgcagc tattggcggg tcgccggcgc ggacggcagc
     2641 cggatcgtga tggattcgcc gccggacaag gaagacgtgc gcccgtggct gcggatccgc
     2701 gacgtgctgg aagccggcgg cgtgcgcgtg ccgcaggtgc tggcgcggga cgtggatgcc
     2761 ggcttcctgc tgctggaaga cctcggcgcc gacacctacc tgcacgtcat cgatgcaacg
     2821 aacgcggacg ccctgttcga cgctgccgtc agccagttgc tgaagctgca ggcgatcgcg
     2881 ccacccgccg acctgcccgc ctacgacgag gcgctgctgg cgcgcgaact gcgcctgttc
     2941 gacgaatggt tctgcggcac ccacctgggc ctgtcgctgc agtgcgccga ggtggacgtg
     3001 ctggaccacg cctaccgggt gctggtcgac gccgcactgg cgcagccgca ggtgctggtg
     3061 caccgcgact tcatgccgcg caacctcatg cccatcaaca gcgggcccgc gaccgatggc
     3121 ccggcggtgc tggatttcca ggacgcggtg cgcggcccga tcgcctacga cgcgctcagc
     3181 ctgttcaagg atgcgttcct cagctggccg gaggatcgcg tggacgcgtg gctggaccgc
     3241 taccacgcgc gtgcggtcgc agccgggctg ccggtcccgg cgctcgcacg cttccgccgc
     3301 gacgcggacc tgatcggcgt gcagcggcac ctgaaggtga tcggcatctt cgcccgcctg
     3361 caccaccgcg acggcaagcc gaagtacctg gccgacgtgc cgcgcttctt cggctacctc
     3421 gaccaggccc tgccgaagta tccggaactc gccggcctgc acgggttcat cgagcagcgg
     3481 gtgaaaccgg ccatggccca acgcgctggc ggcgatcgcg catgaaggca ctgatcttcg
     3541 ccgccggact tggcgagcgc atgcgcccgc tgaccgacac caccccgaag ccgctgctcg
     3601 cggtgggcgg caagccgctg gtggaatggc acctcgaaaa actcgcggcc tgcggggtcg
     3661 aggacgtggt catcaacacc tcgtggctgg cgcagcaatt cccggccacg cttggcgacg
     3721 gcgcgcgctg gggcctgcgc atccgctatt cgtacgaggg cgccaccccg ctggagaccg
     3781 gcggcggcat gtggaacgcg ctgcccctgc tcggcgatgc gcacggctcc aatgaagcgt
     3841 tcctgctggt caacggcgat gtctggacgg atttcgattt cgcccggctg ccccgcgaac
     3901 cgcagggcct ggcccacctg gtgatggtgg atcgcccgcc gcaggcgacc cacggcgatt
     3961 tctcgctggc tgcagatggc gtgatccgcg ccgatggcga gcaccggctg acctattccg
     4021 gcgtcggtgt ctatcgcccg cggttgttcg atggctggcg cgcgatcgtg cccgaaccgg
     4081 ccatcgtcga tggcaagccg aaattcccgc tggcgccgat cctgcgcgcg cacatgggcg
     4141 aaggcatggt ccgcggcgaa caccaccgcg gccgctggac cgacgtcggc accccgcaac
     4201 ggctggcgca actggatgcg gagttggcgg cgcgctaggc gccgagcacc tggcgcagga
     4261 acggcaccgt cagccggcgt tgcgcggcca gcgcggcgtg gtcgaggcgt tcgaacagcg
     4321 cggcaaggtc cgacaggtcg cggctgcacc gcgtgagcag ccactccagc gcggcctcgt
     4381 cgaactgcag gccgcgcgac gccgcgcgct ggcgaaggac ctgcgcacgg ccggcgtcgt
     4441 ccagcgcacg cagcgccagc agggtgcagt gcgcaagccg cgaacgcagg tccggcagct
     4501 gcagcggcag cgcgcccggc agcgcgcgcg cagcgtacgc cagcgccacg ccggcatcgc
     4561 gcacgcggtt gtgcaggtcg aacagggcga tttcgtcgtc gcggttcccg gccagcgcat
     4621 cgaggtcgtc cagcgccacc aggccggcct gctcgatacc ctgcagggca tcgcgcgcat
     4681 gcccgcgcac ccgtgccagc gagaggtagt tggcatggcg gccggcggcc tcggcggcgg
     4741 cacaggcagc cagcagcagg tgggtcttgc cgctgccgct gccgccctgc aggtacaggc
     4801 tgtcgctgct ttcgccggcc gcggtcgcct gcagttgcgc cagtactgcc ggcgccccca
     4861 cccagccttc gaagcgctgg tccggggaat ggcgcaacgc cagtggcaac tgcggacccg
     4921 ctcgatcctg cgcgaccatg ctcaaacgtc gggcggcacg tcgtccgcgc ccggcggtgc
     4981 atcggccgtg cccgtcgcgc catcgggcgc ggcctgttgc cgcacgcctg cgtacagccg
     5041 gctgccggtg tagcgctcat gggcgaaccg cagcagcacg ttggccaccg ccgccaccgg
     5101 caatgccagc agcatgccga ggaatccgaa caggctgcca cccgccatca ccgcgaagat
     5161 caccgccatc ggatgcaggc cgatacggtc gccgaccagc ttcggcgtca gccagtagct
     5221 ttcgatcacc tggccgaccg tgaacaccac ggcaaccccc gccaggtgct gccagtcgcc
     5281 gaactggacc aacgcagcgg tgccgccgaa caccacgatc gcggccggcc cgaggtacgg
     5341 cacgaaggtc agcaggccgg cgatgacccc gatcaggatc cccaggtcca gccccaccgc
     5401 ccacaacccc atcgcgtaca acacgccgag gatcagcatc accagcgtct gcccgcgcag
     5461 gaagccgccc agcaccgcat ccgattcccg cgccagcctg cgcacggtgt cctggtggtc
     5521 gcgcggaacc agcagcccga tccgttccac cagcaggtcc cagtcgcgca ggaagaagaa
     5581 cgtcagcacc ggcagcagca ccaggttggc gaaccaggcg agcagcgcga aaccggaacg
     5641 cgacacgtag ccgagcaccg tcgccgcgac cccgcccgca cgctcccagt gcgcgcggat
     5701 caggttgaac aggtgctccg gatccagcca cgacaggatt tccaggccgg tgcggcgctc
     5761 gaaccatggc agtgcggtgc ccacgaacca gtcgcggtag cgcggcagcg attcgaccag
     5821 ggtcacgacc tgctgctcca gcagcgggac caggaccagc agggccagca ccagcaccgt
     5881 ggtcatcacg ctgaacacca ggatcaccgc ggtgttgcgg gtacggccgg cgcgctccag
     5941 gcgatcgacc agtgggtcgc cgagccaccc cagcagcgcg gcgaacacga acgggctgag
     6001 gatcggcgcc agcagccaga cgatccacag cgcacccagc gccagcgccg cccattgcag
     6061 ccggcggatg aaggtggcga tggtcgccag gtcggggttg tcggtcagca tcgctcagcc
     6121 cccaaggcgg aagctggcga cgcccggtgc cgtcgcggga tcgccgtcct tgccgggccc
     6181 gccgccggtg gagatcgttg acagcacgcc gccgcgatcg accaggcgcg cgaagctgga
     6241 aatcccggtg gccagttcca ggtcgagttg cagttcgccc ggcaatgcca gcaccggccg
     6301 cacctgcctg acgatcgaga ggtcgtcgag gtagcccgcc agccgcatgt agtcgtccgc
     6361 gctgtccacc ccgaggatgc gcacgcgata ggtgccggac ggcccgccgc tgccggcccg
     6421 cgcgtagcgc ttgaacagcg catcggcggc accgtcggcg ccacccacca tggcccggcg
     6481 cgcatcggca tgcttggtct gccagctgga cagggtcttg cccttgtcga ccagggtcca
     6541 gtccgcgacc cagccgccgg tcccgcgctg cagcttgccg atcagctgca tcggcgggct
     6601 gtacttgcgc gaaaggccgg caatggcgcc ggtgtcgccg cgccagatcg cgccaaccgc
     6661 cgcctgctcc gcggcattgc cgccgggcag gcccagcgcg aagccgcgcg ccttggcctg
     6721 gtcgaggatc gcgcgcgccg cattgacctg gcccaggccg accaggcgcg ggccgctgcc
     6781 atcgttgatc gccagccaca gcaccggttt cggccgcggc agcggccacg ccggcaggcc
     6841 cagcatctgc accagctggt cgacatcgtc gggcttgaag cgcgcgacca gggtggtctg
     6901 gaagctcggc gccccgctgc tggaaacacc ttcgtcctgg cggtagtcgt agcccgccac
     6961 gtaatccccg gccttgccga gctcctcgcg cacaccgggc cgctgcgccg cgccacggtc
     7021 gccggtgacc ttcgccagca ccgccgccag cgcgcgcccg aacccggcat tgcgctcggc
     7081 atcgccctgg ttccggaccg ccacctcggc ctcgtagggg ccgcttgcgc cggcgcgatc
     7141 gccctcgacg cgctgggcgt gggcgattcc ggcgcacagc agcaacagga ccgccagcat
     7201 gtggctggcc gcccgcgtcg cccaggattt ccgctgcatc cgttgcatca gcacgcccgt
     7261 ccctgcccgg caatgcgccc ggccaaggtg aaatggtgcc gcagcagcgc gggcgcgtcc
     7321 acgtgaagac aagcggcacg ctaaaatgcg cccctcccag gcgcgttcgc gccagcgcgc
     7381 cgaggctccc cgtgacgcag tccacccccc ccgcagcagc cggcctgacc taccgcgacg
     7441 ccggcgtcga catcgatgcc ggcaatgagg tcgtcgaacg catcaagccg ctggtcaagc
     7501 gcaccctgcg gcccgaagtg ctgggcggcg tgggcctgtt cggcggcctg ttcgacctct
     7561 ccagccgcta ccgcgagccg gtgctggtat ccggcaccga cggcgtgggc accaagctga
     7621 tcctggccaa gcagctgaac cgccacgcca ccatcggcca ggacctggtg gcgatgtgcg
     7681 tcaatgacgt gctggtgcag ggcgccgagc cgctgttctt cctcgactac ttcgccaccg
     7741 gcaagctgga cgtggacacc acggtgcacg tggtgggcgg catcgccagg ggctgcgaga
     7801 tcgccggctg cgcgctgatc ggcggcgaga ccgccgagat gcccgacatg tacccgccgg
     7861 gcgaatacga cctggccggc ttcaccgtcg gcgcggtgga gaagtcgaag ctggtcgatt
     7921 ccggcaagct gcgcgccggc gacgtgctga tcggcatcgc ctccagcggc ccgcattcca
     7981 acggctactc gctggtgcgc aagatcctgg cgcgggccgg catcacgccc gataacggcg
     8041 ggctggacct cgacctcggc ggcgtcaccc tggccgacgc gctgatggag cccacccgca
     8101 tctacgtcaa gccggtgctg gagctgctgg cccgccacga catccatgcg atggcacacg
     8161 tcaccggcgg cgcactggtg gagaagatca tccgggtggt gccggccccg ctgggcctgg
     8221 atatcgacac ctccagctgg ccgctgccgg cggtgttcga ctggctgcag cgcgaaggca
     8281 acgtggcgcg cgaagagatg tggcgcacct tcaactgcgg catcggctac gtgctgatgg
     8341 ccgatcccgc cgatgtggcc gcgatcgaag ccgacctcga ccgcctgcaa ctggcgcact
     8401 ggcggatcgg ccaggtgacg ccaccgcgcg acggcgagcg gctgcgcatc cgctgacgtg
     8461 ccgatgcgcc ttgccgtcct agcctcgggt cgcggcagca acctgcaggc catcctcgac
     8521 gcgatcgccg ccggcaccct ggacgcggag atcgtcggcg tattttccga caagccaacc
     8581 gcgcaagccc tgcgacgcgt gccggagccg ctgcgctggt cgcgcaatgc gaaggattac
     8641 cccaatcgcg ccgcgttcga cgccgaactg gccactgcgg tggcggccac ccggccggac
     8701 tgggtggtgt gcgccggcta catgcggatc ctcggcgacg ccttcgtcca gcgattccgc
     8761 ggccacctgc tgaacatcca cccgtcgctg ctgccccggc accgcggcct gcgcacccac
     8821 gcgggcgcac ttgccgccgg cgatggcgaa cacggcgcca gcgtgcattt cgtggtcccg
     8881 gaactggatg ccggcgcggt gatcgcgcag gccgcgctgc cggtgctggc caccgacacc
     8941 ccggaatcgc tcgccgcgcg ggtgctgcgg ctggagcacc cactgctggt cgcggtcttg
     9001 caaatggcgg cggctggccg catcacggaa caggcggcgc aggccacgct ggatggtcag
     9061 cccctgttta cgccgctgcg actagagttc gccccaggct gaccgcgaag cggccacatc
     9121 cccctttccc ttcaagcagt tgccgatgaa atccgtgcgt gttgccgccc tcctcagcgt
     9181 cctgctcgcc gcctcgccgg cgtgggcggt caagccgttc accgccgact acgaagccag
     9241 cgtcatgggc ggcatttccg ccgacgcgca gatgacgctg gcgtcggccg gcggcgatcg
     9301 ctggaattac gtcctcagcg tggacagccc ggtcgcgacc ctgcgccagg ccaccgtgtt
     9361 cgaggaccgt gatggggcgt ggcggccgct gtccggcaac gattccaccc agatgctgat
     9421 caagaagtcg cagaagaacg ccagctacga ctgggccagg ggcgaggcgc gctggagcgg
     9481 tgacgtcaag gccgaccgtg ccggcccggt caagctgcag gaaggcgacc tcgacgcgat
     9541 gctgctgaac ctcgccatcg tccgcgacgt ggccgcgggc aagccgctca cctaccgcat
     9601 ggtcgacaac ggggtggtgc gcacgcaggc ctaccagaac ctgggcaagg acacggtcac
     9661 ctacgccggc aagccgcgcc aggccaccaa ggtcagccgc agcagcgaga acaagcaggt
     9721 catcgtatgg gtcgtggacg gcctgccggt gcctgcgcgg atcctgcaga agaaggacgg
     9781 caaggacgag ctggacctgg tgctgcagtc cgtgcgctga gccgccccgc cgctccaaca
     9841 aaaagccccg caatgcgggg ctttcctgcg cgcggaggca tcggcctgcc cgggcaggcc
     9901 gatgccgcac cctcatcaat cgacgcggcg gatgctcgcg cccagcgccg acagcttggc
     9961 ctcgatgttc tcgtagccgc ggtcgaggtg gtagatgcgg tcgatgacgg tgtcgccgtc
    10021 cgcgaccagc ccggccagga tcagcgaggc cgaggcgcgc aggtcggtcg ccatcaccgg
    10081 ggcgccgctg agctgcgcga ccccgcgcac cacggccgtg tggccatcca cgcggatgtc
    10141 cgcgcccagc cgcagcagct cgttcacgtg catgaagcgg ttctcgaaga tcgtctcgtt
    10201 gatcacgccg acgccgtcgg cgatgcagtc cagcgccatg aactgcgcct gcatgtcggt
    10261 cgggaacgcc gggtgcgggg cggtggtgag gttgaccgcg cgcggccgct tgccgtgcat
    10321 gtccagcagg atgcggtcgc catcgccggt gccgctttcg atggagatct cggcgccggc
    10381 ctcgcgcagc ttgtccagca ccgcgtccag ggtgtcggcg cgggcgtggg tggcggtgac
    10441 gcggccgccg gtcatcgccg cggccaccag gaaggtgccg gtttcgatgc ggtcggcgac
    10501 caccgcgtgt tcgcagccgg acagcctttc gacgccttcg atggtgatct tgtgggtgcc
    10561 ggcgccggtg atcttcgcgc ccatcgcgac caggcactcg gccaggtcca cgatctccgg
    10621 ctccatcgcc gcgttctcca gcacggtggt gccgtccgcc agcgccgcgg ccatcagcac
    10681 gttctcggtg gcgcccacgc tgaccatctc gaacacatgc cttgcgcctt tcaagcggcc
    10741 ggcactgcgc gccttgatga agccgtggtc gaccgtgatc tcggcgccca gcgcctgcag
    10801 gcccttgatg tgcaggtcga ccgggcgcga gccgatcgcg cagccgcccg gcagcgagac
    10861 ttcggcatcg ccatgcttcg ccagcagcgg ccccagcacc agcaccgagg cgcgcatggt
    10921 cttgaccagc tcgtacggcg cgacatggct gttgacgctg cgcgggtcca cggtcatgac
    10981 gtcgccgcgg tgctcgatgc cggcgcccag cccggccagc agcttggcgg tggtgaacac
    11041 gtcgtgcagg cgcggcacgt tggtgatccg caccggcgca tcggccagca gggtggcgca
//
LOCUS       DAORUK010000061        10995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_6182_length_10995_cov_19.062888, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000061 DAORUK010000000
VERSION     DAORUK010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10995
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6182_length_10995_cov_19.062888"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PK818_05700"
     CDS             complement(<1..542)
                     /locus_tag="PK818_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQY82262.1"
                     /translation="MEAVPGLDLSHPPFDLLDDAGRARLAARVDMGFHPRGETIITAG
                     APSERVVLILKGQVHAFDLDAAGHEQRFADYGSGDIFGAWAVMAGRARHSYRADTDVV
                     SFLIPAGIFRQLLAEQPAFAAWFQEGLAVKGQLAQRGRGSELAELMVTQVGAAHLAPA
                     VRVPAATSIADASARLREERV"
     gene            complement(635..925)
                     /locus_tag="PK818_05705"
     CDS             complement(635..925)
                     /locus_tag="PK818_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82263.1"
                     /translation="MLANAQVQRAGDYLSKMDVDGDGRVSLVEYQDWLGYAFHGMDRD
                     RDGVLSAAEQPGGRGTPLSLAEHRARIAERFARQDTNRDGFLGARELAAPPR"
     gene            complement(1018..2160)
                     /locus_tag="PK818_05710"
     CDS             complement(1018..2160)
                     /locus_tag="PK818_05710"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HQY82264.1"
                     /translation="MLLYLLVVTQVTIFTVTLYLHRSQAHRGVDFHPVIAHFFRFWSW
                     LTTSMITREWAAIHRKHHARCETPEDPHSPMHKGIGTVLWKGGDLYREARLDRASIEQ
                     YGKGCPDDWVERTLYTGHANLGVILLLALSFLLFGAKGVAAWAIQMAWIPFWAAGVIN
                     GLGHWWGYRNFETTDTATNLTPWAVWIGGEELHNNHHAFPSSARFAMRKWEFDIGWAA
                     IRLLQALKLAKVLRVAPSLDVRPNIAMPDGETLKALVAHRFQAMTDYQRNVLKPALRE
                     EAAAAGARLRALLPRKLRKGIVDDGRWLGPDARAQLQAWIDARPRIRTLVEYRAQLAQ
                     VLESRSVDANERLQRLQAWCAEAEASGVRALQEYAARLKGYALQPA"
     gene            complement(2297..2842)
                     /locus_tag="PK818_05715"
     CDS             complement(2297..2842)
                     /locus_tag="PK818_05715"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQY82265.1"
                     /translation="MRNLLVGLLAAILCGGAAMAAPAVAPKTGEVPPGLLGKDRDGNV
                     VDLAQHRGKIVVVTFWASWCGPCRKELPGLEALQQHAGDKLLKVIAVNVKDSTEDYRL
                     MMRQMRGFTMAMTRDRDGKISEGYDVQAFPNLWIIDPRGKVAAHHVGYGEDSLDALVD
                     EINGIIAAEQQRQKAAAATAG"
     gene            2893..3705
                     /gene="mutM"
                     /locus_tag="PK818_05720"
     CDS             2893..3705
                     /gene="mutM"
                     /locus_tag="PK818_05720"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HQY82266.1"
                     /translation="MPELPEVETTRRGLLPHVAGRRVANVLLRRPDLRWPIPREVEAL
                     LPGQRIEGIRRRAKYLLLDTAAGSALLHLGMSGSLRVLPGDTPVRAHDHVDIALDSSR
                     VLRFNDPRRFGCLLWQAPGETHPLLAALGPEPLSDDFDGDLLFLRSRGRSAPVKTFLM
                     DQAVVVGVGNIYAAEALFMAGISPLRAAGKVSRGRYQALADAVKAILAHAIQRGGTTL
                     RDFISPDGAPGYFEQELLAYGRGGEPCPRCGRPLKQAMIGQRASVWCGHCQR"
     gene            3787..4365
                     /locus_tag="PK818_05725"
     CDS             3787..4365
                     /locus_tag="PK818_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HQY82267.1"
                     /translation="MTDSGEITVLLDAAHKGDRGAMDRVLATLYQELHSMARRQLAGQ
                     HGHTLDATALVHEAYLKLIGRGKGAAQFDDRAHFFAYAASAMRSVVVDYARQRLAQKR
                     GGDQHRVTDLPEDIEGGIRLDEDMLGLDTALTRLHAVEPRLTQVVELRYFAGLSELEI
                     AALLQRSERSVRRDWQKARMFLLASLKDPAVA"
     gene            4396..7200
                     /locus_tag="PK818_05730"
     CDS             4396..7200
                     /locus_tag="PK818_05730"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQY82268.1"
                     /translation="MDAERWQRLSPLLDALLELPLEARGEHLAALRREDAALADELEG
                     LLALEEGDDAFLAEPVVEVPNSARPGARIGPYRLDRLIGEGGMGQVWLAARADGLYDR
                     KVALKLLRAGLADPKLRVRFDREREILARFAHPYIARLLDAGIDRDGQPYLALEYVDG
                     EPITSYCQSRQLDIAARLDLFRQVCEAVSHAHANLIVHRDLKPSNILVTPAGHVRLLD
                     FGIAKLLDGEPLPVEQTRTGVRTFTLHYAAPEQIRGEPVTTMTDVYSLGVVLYELLTG
                     SKPYRLKRQTDAEWEEAILAGEPLRPSQAAARASEEAARPYSPARLARELSGDLDNIS
                     LKALGKRPDQRYVSAEALSQDLLCYLRGRPVLARGESLGYRTRKYFNRHRWALGAATL
                     VLAVMVTALVLVSWQARQALREANRAQALQNFVAGLFESAGNTPLDAPMDLRTLLDLG
                     IVRGERSLARQPQARAELYGMVARLRLGLGDYREANALLERQAQLLRALPEAPTSLRL
                     EAATLRGAARRELSDARGCERQMQPLLALAEREQEQLPAQVAAFYSQLARCRRELGER
                     NLANQLFSRSLKLRRDNGDDVGVAESLLDLSALHADDGNPRQAIRVLRDALAQFRQKV
                     GDRHPLAIDMLRSLCSLERNNDDLAAAERDCRASLALAQELHGREHRATIDARRQLAA
                     LYVDLGRFGEAESEFLDTYAWMRARLQRNHPDLARLYNSLGVVAWERGDLKRAIAYQT
                     QAVEAWRSDPNPGTIAAGTFNLAMILHSDGQDARALPLVRESRQLRVQRFGPGHELVG
                     DSDRLLGEVLAALGQDDAAREALQDAVRLTRAGYGPAHSHTRRAEISLARFEAARGNA
                     EALQRLRGWANPDQRDIEQRKAGWLARAYAAERDCAARPAQAGGELDAVLAQVQLLLP
                     EGGALPREIGAIRAACGKRG"
     gene            complement(7218..7838)
                     /locus_tag="PK818_05735"
     CDS             complement(7218..7838)
                     /locus_tag="PK818_05735"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019338127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HQY82269.1"
                     /translation="MAKLYFYFSAMNAGKTTTLLQSAHNYRERGMRVAILTPRLDDRA
                     GQGVVASRIGLQAEGIAFTGSADLEALVRADIAARGKLDCVLVDEAQFLTKAQVWQLS
                     EVVDALRIPVLCYGLRTDFRGELFEGSQYLLAWADELQEIKTICHSGKKATMNVRIDA
                     QGHALQDGPQVEIGGNERYVSVSRAEFKKVMRGEGRVLPLQAPLPL"
     gene            7903..9897
                     /locus_tag="PK818_05740"
     CDS             7903..9897
                     /locus_tag="PK818_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQY82270.1"
                     /translation="MRNPALHGLNPPQQQAVLHTEGPLLVLAGAGSGKTRVIIEKIAH
                     LIHTGRYPAKRIAAITFTNKSAREMRERVARRIRGDAAQDLTVSTFHALGLRILQVEH
                     ARLGLRRGFSIFDADDSAAQLKDLLPPGSKPDVVDAAKQLIGRAKNAGLSPQQAAEAA
                     RSAREHEAAALYARYQQRLSAFNAVDFDDLIRLPVQLLESDADAVAGWRERIGYLLVD
                     ECQDTNDAQYRLLKAIAGEPGQFTVVGDDDQSIYAWRGANPDNLLQLGSDYPALRIVK
                     LEQNYRCSNRVLRAANALIANNPHEHPKTLWSDAADGERIRVWECRDAAHEAEKVAAE
                     IQFTAQKHAAPWSEFCILFRGNHQSRALEKSLQLLRIPYHLSGGTAFLDRGEVKDALA
                     WLRLLANPDDDAAFLRAVAAPNRGVGATSLAKLAELAAHAHLPLARAAESMAVAKQLP
                     ARAANALQGFTDIVRGLRGDARTLPPAELVRRLNERSGLLAALRALCKDEAQFQARRR
                     NLDELADWFDGPRTSGPGELAAALALLSHADKGDAGNQVRLMSMHAAKGLEFRFVFIV
                     GVEDGNLPHEASIEEGRIDEERRLLYVGITRAREQLWLSHASQAQRWGDRLRLRPSRF
                     LDELPAAELQRDGADPVADAARKAERASAGIANIRALLGE"
     gene            9964..10884
                     /locus_tag="PK818_05745"
     CDS             9964..10884
                     /locus_tag="PK818_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HQY82271.1"
                     /translation="MRPALTLSLLAAALSLAACKPASFTAPDTATQPATPAATAAAAP
                     AAAGAGPNDNLNAVLWVQRAAEYDAVAQTVYRAAADKLDAALTQANWDALVPDERGNA
                     ATGLPPAVVMDVDETVLDNSPYQARLVRDGASYEDTTWDLWVAEKKAKAVPGVVDFAK
                     AAAARGVTILYISNRAEHLQAATIANLKAEGLPVKDESVFLGLGTFVADCEQNGSEKL
                     CRRKLAGQKYRVLMQFGDQLGDFVEIVANTPKDRDGLYGEYEDWFGERWFMLPNPTYG
                     SWEPATFNNAWDQPPGARRAAKLGSLDMAK"
BASE COUNT         1642 a         3995 c         3822 g         1536 t
ORIGIN      
        1 acgcgttcct cgcgcaggcg ggcgctggcg tcggcgatgc tggtcgcggc cggcacccgc
       61 accgccggcg cgaggtgcgc ggcgccgacc tgggtgacca tcagttcggc gagttccgat
      121 ccgcgcccgc gctgcgccag ctggcccttc accgccaggc cttcctggaa ccacgcggcg
      181 aacgccggct gctccgccag cagctggcgg aaaattccgg cggggatcag gaagctgacc
      241 acgtcggtgt ccgcgcggta gctgtggcgc gcgcggccgg ccatcaccgc ccacgcgccg
      301 aagatgtcgc cactgccgta gtcggcgaag cgctgctcgt ggccggcggc gtccaggtcg
      361 aacgcatgca cctggccctt caggatcagc acgacgcgtt cggacggcgc accggcggtg
      421 atgatggtct cgccgcgcgg atggaagccc atgtccaccc gcgccgccag ccgtgcgcgg
      481 ccggcatcgt cgagcaggtc gaagggcgga tggctcaggt ccagcccggg cacggcctcc
      541 attgctgcgc tcccgcgagg catgaagcgg tgatcatcgc agccgcgcgc gcgcgcggcc
      601 attccacctt cggcgcatgc gggcggttcc gccgtcagcg cggtggcgcg gccagctcgc
      661 gtgcgcccag gaagccgtcg cgattcgtgt cctggcgggc gaaacgttcg gcgatgcgcg
      721 cgcggtgctc ggcaaggctc aacggcgtgc cgcggccacc gggttgttcg gccgccgaca
      781 gcacgccgtc gcggtcgcgg tccatgccgt ggaaggcgta accaagccag tcctggtatt
      841 cgaccagcga gacgcggccg tcgccatcga catccatctt cgacaggtag tcgccggcgc
      901 gttgcacctg cgcgttcgcc agcatcggaa gcgaacatgc cagcaacagg caaggggcca
      961 gggtgaagtt tcgcgtaacg aaattcaccc tggcccctct cctggcgacg gacccggtca
     1021 cgccggctgc agcgcgtaac ccttcaggcg cgccgcgtac tcctgcagcg cgcgcacgcc
     1081 gctggcctcg gcttcggcgc accaggcctg caggcgctgc aggcgctcgt tggcgtccac
     1141 gctgcggctt tccagcacct gcgccagttg cgcgcggtat tcgaccaggg tccggatccg
     1201 cggccgcgcg tcgatccacg cctgcaactg cgcacgcgca tccgggccca gccagcggcc
     1261 gtcgtcgacg atgcccttgc gcagcttgcg cggcagcagc gcgcgcagcc tggcgccggc
     1321 ggccgcggct tcctcgcgca gggccggctt cagcacgttg cgctggtaat cggtcatcgc
     1381 ctggaaacgg tgcgcgacca gcgccttcag ggtctcgcca tccggcatcg cgatgttcgg
     1441 gcgcacgtcc aggctgggcg ccacccgcag gaccttcgcc agcttcagcg cctgcagcag
     1501 ccggatcgcc gcccagccga tgtcgaattc ccacttgcgc atcgcgaacc tggccgacga
     1561 cgggaacgca tggtggttgt tgtgcagttc ctcgccgccg atccacaccg cccacggggt
     1621 gaggttggtg gcggtgtcgg tggtctcgaa gttgcggtag ccccaccagt ggccaagccc
     1681 gttgatcacg ccggcggccc agaacgggat ccacgccatc tggatcgccc atgcggccac
     1741 gcccttggcg ccgaacagca ggaagctcag cgccagcagc aggatcacgc ccaggttggc
     1801 atggccggtg tagagcgtgc gctcgaccca gtcgtccggg cagcccttgc cgtactgctc
     1861 gatgctggcg cggtccaggc gcgcctcgcg gtacaggtcc ccgcccttcc acagcacggt
     1921 gccgatgccc ttgtgcatgg ggctgtgcgg gtcttccggg gtctcgcacc tggcgtggtg
     1981 cttgcgatgg atcgccgccc actcgcgggt gatcatcgag gtggtcagcc agctccagaa
     2041 cctgaagaaa tgcgcgatca ccgggtggaa gtccaccccc cgatgcgcct ggctgcggtg
     2101 caggtacagg gtcacggtga agatggtgac ctgggtcacc accagcaggt acagcagcat
     2161 cgccggccag ccgaactgga gcaggccacc ggcaaggaaa tcgatcaggg acgcaggcat
     2221 cgtggggggg tcttggaagg cgtgggggga cgctggggga tgatgccacg gcccccggcg
     2281 cggccgctga acgatgtcag cccgcggtcg cggcggcggc cttctgccgt tgctgctcgg
     2341 cggcgatgat gccgttgatc tcgtcgacca gggcgtcgag cgagtcctcg ccatagccga
     2401 cgtggtgcgc cgccaccttg ccccgcggat cgatgatcca caggttgggg aatgcctgga
     2461 cgtcgtagcc ttcggagatc ttgccgtcgc ggtcgcgggt catggccatg gtgaagccgc
     2521 gcatctgccg catcatcagg cggtagtcct cggtgctgtc cttcacgttg accgcgatca
     2581 ccttcaggag cttgtcgccg gcatgctgct gcagcgcctc caacccgggc agctccttgc
     2641 ggcagggtcc gcaccaggat gcccagaagg tcaccaccac gatcttgccg cggtgctggg
     2701 ccaggtcgac gacgttgccg tcccggtcct tgccaagcag ccccggcggc acctcgcccg
     2761 tcttcggcgc caccgccggt gcggccatcg cggcgccacc gcacaagatc gcggccagca
     2821 ggccgaccag caagttccgc atatgctttc cccggatgaa gtccgcgaag tctaatcaac
     2881 cggtggtcgg cgatgcctga actgcccgag gtcgaaacca cccgccgcgg cctgctgccg
     2941 catgtcgccg gccgccgggt cgcgaacgtg ttgctgcggc gccctgacct gcgctggccg
     3001 atcccgcgcg aggtcgaagc gctgctgccc ggacagcgga tcgagggcat ccgccggcgc
     3061 gccaagtacc tgctgctgga caccgcggcc ggcagcgcgc tgctgcacct gggcatgtcc
     3121 ggcagcctgc gggtgctgcc cggcgatacc ccggtgcgtg cgcacgacca tgtcgacatc
     3181 gcgctggatt cgtcccgggt gctgcgcttc aacgatccgc gcaggttcgg ctgcctgctg
     3241 tggcaggcgc cgggcgaaac ccacccgctg ctggccgcgc tcgggccgga acccttgtcc
     3301 gacgatttcg acggcgacct gctgttcctg cgcagccgcg gccgcagcgc gccggtgaag
     3361 accttcctga tggaccaggc ggtggtggtc ggggtcggca acatctacgc cgccgaggcg
     3421 ctgttcatgg ccgggatctc gccgctgcgg gccgccggca aggtctcgcg cgggcgttac
     3481 caggcgctgg ccgacgcggt gaaggcgatc ctggcccacg ctatccagcg cggcggcacc
     3541 accctgcgcg atttcatcag cccggacggc gcccccggct atttcgagca ggaactgctg
     3601 gcctacggtc gtggcggcga gccgtgcccg cgctgcgggc ggccgctgaa gcaggccatg
     3661 atcggccagc gcgccagcgt gtggtgcggc cattgccagc gctgacccgc ggcccccgcg
     3721 gcgcttgcgc gggtctggcc gctccgggct atagtccacg ttcccccgtt tcccgttgcc
     3781 gtggcgatga ccgactccgg tgaaatcacc gtcctgctgg atgccgcgca caagggcgac
     3841 cgcggcgcga tggaccgcgt gctggccacc ctgtaccagg aactgcattc gatggcgcgc
     3901 cgccagctgg ccggccagca cgggcacacc ctggatgcca ccgcgctggt gcacgaggcc
     3961 tacctgaagc tgatcggccg cggcaagggc gccgcgcagt tcgacgaccg cgcgcacttc
     4021 ttcgcctatg cggcctcggc gatgcgcagc gtggtggtgg attacgcccg ccagcggctg
     4081 gcgcagaagc gcggtggcga ccagcaccgg gtcaccgacc tgccggagga catcgagggc
     4141 ggcatccgcc tggacgagga catgctgggc ctggacaccg cgctgacccg cctgcacgcg
     4201 gtcgaaccgc ggctgaccca ggtggtcgaa ctgcgctatt tcgccggcct gtccgagctg
     4261 gagatcgccg cgctgctgca gcgctccgag cgcagcgtgc gccgcgactg gcagaaggcg
     4321 cggatgttcc tgctcgcctc gctgaaggac ccggccgtcg cctgagcccg gggccaggcg
     4381 atccggcgca gaccaatgga cgccgaacgc tggcaacgcc tgtcgccgct gctggatgcg
     4441 ctgctggagc tgccgctcga agcgcgcggc gaacacctgg ccgcgttgcg ccgcgaggac
     4501 gcggcgctgg ccgatgaact cgagggcctg ctcgcgctgg aggaaggcga tgacgcgttc
     4561 ctcgccgagc cggtggtgga ggtgcccaac agcgcgcgcc cgggcgcgcg gatcggaccg
     4621 taccggctgg accggctgat cggcgaaggc ggcatgggcc aggtctggct ggcggcgcgc
     4681 gccgacggcc tgtacgaccg caaggtcgcg ctgaaactgc tgcgcgccgg cctggccgat
     4741 cccaagctgc gcgtgcgctt cgaccgcgag cgcgagatcc tggcccgctt cgcgcacccc
     4801 tacatcgcgc gcctgctgga cgccggcatc gaccgcgacg ggcagccgta cctcgcgctg
     4861 gaatacgtcg acggcgagcc gatcaccagc tactgccagt cgcgccagct cgacatcgcc
     4921 gcgcggctcg acctgttccg ccaggtctgc gaagcggtca gccacgcgca cgccaacctg
     4981 atcgtgcacc gcgacctgaa gccgtccaac atcctggtga ccccggccgg tcacgtgcgc
     5041 ctgctcgact tcggcatcgc caagctgctc gacggcgagc cgctgccggt cgagcagacc
     5101 cgcaccggcg tgcgcacctt caccctgcac tacgcggcgc cagagcagat ccgcggcgag
     5161 ccggtcacca ccatgaccga cgtgtattcg ctgggggtgg tgctgtacga actgctgacc
     5221 ggcagcaagc cgtaccgcct caagcgccag accgacgccg aatgggagga ggcgatcctg
     5281 gccggcgagc cgctgcggcc gtcgcaggcg gcggcgcgcg ccagcgagga ggcggcgcgg
     5341 ccgtattcgc cggcacggct cgcgcgcgaa ctgtcgggcg acctcgacaa catcagcctg
     5401 aaggcgctcg gcaagcgccc ggaccagcgc tacgtctccg ccgaggcgct gtcgcaggac
     5461 ctgctctgct acctgcgcgg gcggccggtg ctggcgcgcg gcgagagcct gggctaccgc
     5521 acccgcaagt acttcaaccg ccaccgctgg gcactgggcg cggcgaccct ggtgctggcg
     5581 gtgatggtga ccgcgctggt gctggtcagc tggcaggcgc gccaggccct gcgcgaggcc
     5641 aatcgcgcgc aggccttgca gaacttcgtg gccggcctgt tcgaaagcgc cggcaacacg
     5701 ccgctcgacg cgccgatgga cctgcgcacc ctgctcgacc tgggcatcgt gcgcggcgaa
     5761 cgcagcctgg cgcggcaacc gcaggcacgc gcggagctgt acggcatggt cgcgcggctg
     5821 cggctcgggc tgggcgacta ccgcgaggcc aacgccctgc tcgaacgcca ggcgcagctg
     5881 ctgcgcgcgc tgccggaggc gccgaccagc ctgcgcctgg aagcggccac cctgcgcggg
     5941 gccgcacgcc gcgaactcag cgacgcgcgc gggtgcgaac gccagatgca gccgttgctg
     6001 gccttggccg agcgcgagca ggagcagctg ccggcgcagg tcgccgcgtt ctattcgcag
     6061 ctggcgcgct gccgccgcga gctcggcgag cgcaacctcg ccaaccagct gttctcgcgc
     6121 tcgctgaagc tgcgccgcga caacggcgat gacgtgggcg tggccgaaag cctgctcgac
     6181 ctgtccgcgc tgcacgccga cgacggcaac ccgcggcagg cgatccgggt cctgcgcgat
     6241 gccctcgcgc aattccggca gaaggtcggc gaccgccatc cgctggcgat cgacatgctg
     6301 cgcagcctgt gttcgctgga gcgcaacaac gacgacctcg ccgccgccga acgcgactgc
     6361 cgcgcctcgc tggcgctggc gcaggagctg catggccgcg agcatcgcgc caccatcgac
     6421 gcgcgccgcc agctggccgc gctgtacgtc gacctcggcc gcttcggcga ggccgaatcc
     6481 gaattcctcg acacctacgc gtggatgcgc gcgcgcctgc agcgcaacca tccggacctc
     6541 gcccgcctct acaacagcct gggcgtggtt gcctgggaac gcggcgacct gaagcgcgcg
     6601 atcgcgtacc agacgcaggc ggtggaggcc tggcggtccg atcccaaccc cggcaccatc
     6661 gccgccggca ccttcaacct ggcgatgatc ctgcacagcg acggccagga cgcgcgcgcg
     6721 ctgccgctgg tccgcgagtc ccgccagctg cgcgtgcagc gcttcggccc cggccacgaa
     6781 ctggtcggcg acagcgaccg cctgctcggc gaagtgctgg ccgcgctcgg ccaggacgat
     6841 gccgcacgcg aggcattgca ggacgcggtg cggctgaccc gcgccggcta cggcccggcg
     6901 cactcgcaca cccgccgggc ggagatctcg ctggcccgct tcgaagccgc gcgcggcaac
     6961 gccgaggcgc tgcaacggct gcgcgggtgg gcgaacccgg atcagcgcga catcgaacag
     7021 cgcaaggccg gctggctggc gcgcgcctat gccgccgaac gcgattgcgc ggcgcggccg
     7081 gcgcaggccg gcggcgaact ggatgcggtg ctggcgcagg tccagctgct gctgcccgaa
     7141 ggcggcgcgc tgccgcgcga gatcggcgcg atccgcgcgg cctgcggcaa gcgcgggtga
     7201 ccgccggcgt ttcccgccta cagcggtagc ggcgcctgca gcggcaggac cctgccctcg
     7261 ccgcgcatca ccttcttgaa ttcggcgcgg ctcaccgaca cgtagcgctc gttgccgccg
     7321 atctccacct gcgggccgtc ctgcagcgca tgcccctgcg catcgatgcg cacgttcatg
     7381 gtcgccttct tgccgctgtg gcagatggtc ttgatctcct gcagctcgtc ggcccacgcc
     7441 agcaggtact ggctgccctc gaacagctcg ccgcggaaat cggtgcgcag gccgtagcag
     7501 agcaccggga tgcgcagcgc atccaccacc tcgctcagtt gccacacctg cgctttcgtc
     7561 aagaactgcg cctcgtcgac cagcacgcaa tcgagcttgc cgcgggcggc gatgtccgcg
     7621 cgcaccagcg cctccaggtc ggcgctgccg gtgaaggcga tgccctccgc ctgcaggccg
     7681 atccgcgagg ccaccacccc ctggccggcg cgatcgtcca gccgcggggt caggatcgcc
     7741 acccgcatgc cgcgctcgcg gtagttgtgc gcggactgca gcagggtggt ggtcttgccg
     7801 gcgttcatcg ccgagaagta gaagtagagc ttggccatgc cgcgattcta atgtcggcgc
     7861 gtccgcgggg cgcgcttgcc ggcgacgtac aatgcgcgcc ccatgcgcaa cccagcactc
     7921 cacggcctga acccgccgca gcagcaggcc gtcctccaca ccgaaggccc gctgctggtg
     7981 ctcgcgggcg cgggcagcgg caagacccgg gtgatcatcg agaagatcgc ccacctgatc
     8041 cataccggcc gctatcccgc caagcggatc gcggcgatca ccttcaccaa caagtccgcg
     8101 cgcgagatgc gcgagcgcgt ggccaggcgg atccgcggcg atgcggcgca ggacctgacc
     8161 gtcagcacct tccacgcgct gggcctgcgc atcctgcagg tcgagcacgc ccgcctcggc
     8221 ctgcgccgcg gcttttcgat cttcgacgcc gacgattccg cggcgcagct gaaggacctg
     8281 ctgccgccgg gcagcaagcc ggacgtggtg gatgccgcga agcagctgat cggccgcgcc
     8341 aagaacgccg ggctgtcgcc gcaacaggcc gccgaggccg cccgcagcgc ccgcgaacac
     8401 gaggcggcgg cgctgtacgc gcgctaccag cagcgcctgt cggcgttcaa tgcggtcgat
     8461 ttcgatgacc tgatccgctt gccggtgcag ttgctggaat ccgatgccga tgccgtggcc
     8521 ggctggcgcg agcgcatcgg ctacctgctg gtcgacgagt gccaggacac caacgacgcc
     8581 cagtaccggc tgctgaaggc gatcgccggc gaacccggcc agttcaccgt ggtcggcgac
     8641 gacgaccaga gcatctatgc ctggcgcggc gccaacccgg acaacctgct gcaactcggc
     8701 agcgactacc cggcgctgcg catcgtcaag ctggagcaga actaccgctg cagcaaccgg
     8761 gtgctgcgcg cggccaacgc gctgatcgcc aacaacccgc acgaacaccc gaagacgctg
     8821 tggagcgatg ccgccgatgg cgagcggatc cgcgtctggg aatgccggga tgccgcccac
     8881 gaggcggaga aggtcgccgc agagatccag ttcaccgcgc agaagcacgc cgcgccgtgg
     8941 agcgagttct gcatcctgtt ccgcggcaac caccagagcc gcgcgctgga gaagtcactg
     9001 caactgctgc gcatccccta ccacctgagc ggcggcaccg ccttcctcga ccgcggcgag
     9061 gtcaaggacg cgctggcgtg gctgcgcctg ctcgccaatc cggacgatga cgccgccttc
     9121 ctgcgcgcgg tggccgcgcc gaaccgcggc gtcggcgcca cctcgctggc gaaactggcc
     9181 gaactggccg cgcatgcgca cctgccgctg gcgcgtgcgg ccgagtcgat ggcggtggcc
     9241 aagcagctgc cggcgcgcgc cgcgaacgcg ctgcagggct tcaccgacat cgtccgcggc
     9301 ctgcgcggcg acgcccgcac gttgccgccg gccgaactgg tgcgcaggtt gaacgaacgc
     9361 tccggcctgc tcgccgcgct gcgcgcgctg tgcaaggacg aggcgcagtt ccaggcgcgc
     9421 cggcgcaacc tggacgagct cgccgactgg ttcgacggcc cgcggacgtc gggtcccggc
     9481 gagctcgccg ccgcgctcgc cctgctctcg cacgcggaca agggcgatgc cggcaaccag
     9541 gtgcggctga tgtcgatgca cgcggcgaag gggctggagt tccgcttcgt gttcatcgtc
     9601 ggggtggagg acggcaacct gccgcacgag gccagcatcg aggagggccg gatcgacgag
     9661 gaacgccggc tgctgtacgt cggcatcacc cgcgccaggg aacagctgtg gctctcgcac
     9721 gccagccagg cgcaacgctg gggcgaccgc ctgcggctgc ggccgagccg cttcctggac
     9781 gagctgccgg cggccgaact gcagcgtgac ggcgccgacc cggtggccga tgccgcacgc
     9841 aaggcggagc gcgccagcgc cgggatcgcc aatatccgcg ccctgctggg cgagtgatcc
     9901 gccgcctggg cggtcaccac gggcaggtac actcggcccg gaacccgtcc acgagtcctg
     9961 tccatgcgtc ctgccctgac cctgtccctg ctcgccgccg cactgtcgct ggcggcctgc
    10021 aagcccgcgt ccttcaccgc gcccgatacc gcaacgcaac cggccacgcc cgcagccacc
    10081 gcggcggcgg cgccggcagc ggccggcgcc ggcccgaacg acaacctcaa cgcggtgctg
    10141 tgggtgcagc gcgcggccga gtacgacgcg gtcgcgcaga ccgtgtaccg cgccgccgcc
    10201 gacaagctgg acgcggcact gacgcaggcc aactgggacg cgctggtgcc ggatgaacgc
    10261 ggcaacgccg ccaccgggct gccgccggcg gtggtgatgg acgtcgacga gaccgtgctc
    10321 gacaactcgc cctaccaggc gcggctggtg cgcgacggcg ccagctacga agacaccacc
    10381 tgggacctgt gggtcgccga aaagaaggcg aaggcggtcc ccggcgtggt cgatttcgcc
    10441 aaggccgcgg cggccagggg cgtgaccatc ctgtacatct ccaaccgcgc cgagcacctg
    10501 caggcggcga ccatcgccaa cctcaaggca gagggcctgc cggtcaagga cgaaagcgtg
    10561 ttcctcggcc ttggcacctt cgttgcggac tgcgagcaga acggcagcga gaaactctgc
    10621 cgtcgcaagc tggccggcca gaagtaccgc gtgctgatgc agttcggcga ccagctcggc
    10681 gatttcgtcg agatcgtggc gaacacgccg aaggaccgcg acggcctgta tggcgaatac
    10741 gaggactggt tcggcgagcg ctggttcatg ctgcccaacc cgacctacgg cagctgggag
    10801 ccggcaacgt tcaacaacgc ctgggaccag ccgcccggcg cgcgccgcgc ggcgaagttg
    10861 ggaagtttgg atatggccaa ataaatcaat ggtttatctg ataaacattc atctattgca
    10921 ttaagtcaat gacggcggta gcctgcatcc atccggcctc ggccggagcc atgccacaac
    10981 tgctgtcctc cggcc
//
LOCUS       DAORUK010000062        10558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_6609_length_10558_cov_20.170618, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000062 DAORUK010000000
VERSION     DAORUK010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10558
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6609_length_10558_cov_20.170618"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PK818_05750"
     CDS             complement(<1..616)
                     /locus_tag="PK818_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HQY82272.1"
                     /translation="MDAKYWLLGALAVSGMGMGDAAAQVTAADYERAAKMLGDRTAPL
                     VDGGVRGVAWLDDGSVVYGASAGGKTAWLRFDPASGTPAPAFDHKALAAAIDKANPKG
                     KPADPDKLMAGGFKRDGDALRIDLRGSAYRCDAAGCAALAKPASGTEPGAASPDGSRA
                     AFVRDWNLWLRDLASGKETQLTFDGKPDYGYATDNAGWKHTDNAI"
     gene            720..1553
                     /locus_tag="PK818_05755"
     CDS             720..1553
                     /locus_tag="PK818_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQY82273.1"
                     /translation="MENVVTAPALTPERIAVVIPALNEALRIRDVVEGALRHCPNVIV
                     IDDGSDDGTATALAGLPVTVLRHARRMGKGAGLRDGFAEALRRGFDAVIAMDGDGQHS
                     GDDIPRLLATGNRFRGWIVIGARLRKRANQPRHRRLANRFGDWGIAWGTGYQVADSQS
                     GQRLYPADVCRLGCSGMPGEDFVFEAQILMSAAQQLGTRCVSVPIEARYNQVHGAPDF
                     RPSHFRPLRDLWRITSHIVLQCVRRGGLWEVHKRVRQHPPVIDDPSGEFAGVQARPQA
                     R"
     gene            1594..2595
                     /locus_tag="PK818_05760"
     CDS             1594..2595
                     /locus_tag="PK818_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine-dependent deoxygenase"
                     /protein_id="HQY82274.1"
                     /translation="MHALNDSLPHPAAASVRVAYRHAPLDMLLADPCLLAVFGFGDAA
                     PAQHDDPRYLHVALQALGTAPFECWSVAGNVRHGRDDGIAWSADATLQFGALEVADDG
                     DVEAASAQAYQRLQAWLAASGYPHPLRLWNYMDAITEGEGDGERYRRFCVGRARGIGR
                     ELAPAELPAATAIGDPRRSGRFQLYWLAARAAGTALENPRQTQAWRYPRQYGPQAPGF
                     ARAMLPANESMPLLLSGTAAVVGHASQHGDSLAAQLDETLANLDSLVATARALRPGLA
                     ARLGAGSPLKVYVRDADALAQVAALLDQRLPSMVPRLVLHGHVCRRELAVEIDGVHA"
     gene            2655..2903
                     /locus_tag="PK818_05765"
     CDS             2655..2903
                     /locus_tag="PK818_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019302431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82275.1"
                     /translation="MGLISVLWGIVAMLWMVLAFIPLLGWGNWFMIPFAVVGAIIAAI
                     GIAITPREGRGRAKAGLVLNVVAIVVGAIRLSMGGGVL"
     gene            3000..3398
                     /locus_tag="PK818_05770"
     CDS             3000..3398
                     /locus_tag="PK818_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82276.1"
                     /translation="MPRPFRSAGRNRCRIKGIQSMSANTRNRCLAASLAIAALAAGSA
                     QAAKPMNVLEKKAAAEAMALAYQQRAATKQVARKTAPTTLAAPGGGTGTEVPDDLHNY
                     LHAQVDAQGSVRIVETDGPTAPAKQEANHE"
     gene            3391..5001
                     /locus_tag="PK818_05775"
     CDS             3391..5001
                     /locus_tag="PK818_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HQY82277.1"
                     /translation="MSKSPRLALATAVLVALAGLSQANAADIVPVIFDAAGTGYNDTT
                     PVAPVGGNPGTTLGEQRRIVANYAASLWGSVLQSNVPVYVGARFTALAPGVLGSAGAT
                     AVHANFANAPVASTWYHTALADSIAGIDLRPGFTDISSNFSTAYPFYYGLDGKTPTGQ
                     VNFLDVVMHEYAHGLGFSNFETEATGAFLSGVPDIYSAFTYDNTAGKYWTAMTVAERA
                     TSGINTGNVVFTGPRATAGAQLLLDKRLYFRASAPAAVAGDYQYGTATFGPAASPANF
                     NGSLALATDGVGTTGDGCEPITSNVVGKVALMDRGNCGFAIKAKNAQNAGASAVVIAD
                     NVAGAVAGMSGADPTITIPSVRITLADGNLFKANLPVSLGFVADPVLLQGADSLGRPR
                     LFMPNPVQAGSSGSHYDSVAMPNLLMEPAINDSLRSYYNLDITPALMADIGWKLNTGN
                     AKIGSPRVADCDTGIKAVSEGGLIVGGTVQANNDMCLLSAQTRWGYYSCMQASRDRML
                     AASLITTTQASKMMTCARRVADNTAFPL"
     gene            5078..6070
                     /gene="hemB"
                     /locus_tag="PK818_05780"
     CDS             5078..6070
                     /gene="hemB"
                     /locus_tag="PK818_05780"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009594638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HQY82278.1"
                     /translation="MTYPYSRPRRMRRDEFSRRLMRETVLTANDLIYPVFVHELAGRE
                     AIASMPGIERLSIDELLRVAEQASELKVPALALFPVTAPEAKSLDAVAAWDDAGLCQR
                     AVRALKQRFPELGVITDVALDPYTSHGQDGLVDDSGYVMNDETVEALVRQALSHARAG
                     ADVVAPSDMMDGRIGAIRGELEAEGFVHTRILAYSAKYASAFYGPFRDAVGSAGALGK
                     GNKYTYQMDPANSDEALREIALDLDEGADMIMVKPGMPYLDIVRRAKDEFGAPTFVYQ
                     VSGEYAMLKAAAHNGWLDERACALEALASIKRAGADGVLTYFALDAARWLRETH"
     gene            6096..6938
                     /gene="aroE"
                     /locus_tag="PK818_05785"
     CDS             6096..6938
                     /gene="aroE"
                     /locus_tag="PK818_05785"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011257342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HQY82279.1"
                     /translation="MRKHHAVFGTPISHSLSPRIHAAFARQCGIELDYLAIEADAGDF
                     RAKLDDFAAHGGTGANVTLPLKEAAFALCSEASERARRAGAVNTLVRAGDGWHGDNTD
                     GAGLVRDLTGRHMLDLRARRTLLLGAGGAARGIAPALLEAGIGELTIVNRTPERADAL
                     ADALGEPGRVHPRYLGDLAALGEFDLIVNATSAARGGQLPALPRSLVGRRCAAVDLSY
                     GEAAIPFLAWARANGCHDAVDGLGMLVEQAAESFALWLGTRPETDGVYAELSGRAAAL
                     VTAD"
     gene            complement(7084..7614)
                     /locus_tag="PK818_05790"
     CDS             complement(7084..7614)
                     /locus_tag="PK818_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HQY82280.1"
                     /translation="MHIRPYRDISPTLGARAYVDPAACVIGDVVLGDDVSVWPFTVIR
                     GDVNFVRIGDRTNIQDGTVIHVSHDGPHAKLGGFATRIGSDVTIGHKAIIHACTIEDA
                     VLIGMGAIVLDGAVVKKHAFIGAGALVAPGKVVGEGELWLGNPAKKARLLSEAEIEAL
                     YYSAGHYVRLKDEYLG"
     gene            7653..9065
                     /locus_tag="PK818_05795"
     CDS             7653..9065
                     /locus_tag="PK818_05795"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HQY82281.1"
                     /translation="MDNPADSATSDLAPTLARLRAAWQQRKPDHAQRRGDLQRLRDAL
                     KGRLDEMADAIAADFGHRSKDESRIADGMTVLNDIDHLLKHLRGWMKPRRVGVGWRFL
                     PARAQLRPVPLGVVGVIAPWNYPVNLALIPLATAIAAGNHVYLKPSEHTPRTSEFLRR
                     LLAEVFPADRVAVALGGAEVGAAFAALPFDHLLFTGSTAVGRKVMAAAAPNLTPLTLE
                     LGGKSPAIIADDYPVELAAARIATGKWFNCGQTCIAPDYVLVPAARRDALVAALRAQV
                     HARYGAALSNLGDYTRIVNDGQYARLQGYLDDARARGVEVVTLAGAADAAQRVLPPTL
                     LLDPGDDARVMQDEIFGPLLPLKSYRTLDEAIAYVNAHDRPLALYPFSNHRQTVEKIL
                     HATLSGGVSVNDTLFHFAIGGLPFGGVGPSGMGAYHARAGFDAMSKQLPVLWQARWTG
                     GDLLKPPYSKAKWLLDLIVR"
     gene            9106..9876
                     /gene="xth"
                     /locus_tag="PK818_05800"
     CDS             9106..9876
                     /gene="xth"
                     /locus_tag="PK818_05800"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQY82282.1"
                     /translation="MKIASWNVNSLNVRLPHLQRWLDEAKPDVVALQETKQEDHKFPE
                     DALLELGYRSVFSGQKTYNGVAIISRLPFTGECVNAIPGFDDPQKRVLAATVGDARIV
                     DLYVVNGEAVGSGKFDYKLRWLAAVREWLRAEIAAHPNLVVLGDFNIAPDDRDVFDPR
                     RWREKVLCSSPEREAYRALLELGLHDSFRLFNDEAGHHSWWDYRLSGFERGWGLRIDL
                     VLVSEALKARCVAAGIDREPRSWERPSDHTPVWVEIGP"
     gene            9923..10489
                     /locus_tag="PK818_05805"
     CDS             9923..10489
                     /locus_tag="PK818_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HQY82283.1"
                     /translation="MDGTRDFSIDAMQCRWQHLPGTARARDIAIHWLRVVAGDAAADS
                     LHRDARNRPRLLHGDTSWSHSHARLLVAYTPRGRVGVDIESRVRGTDPLRIARRYFAP
                     EETAALAALDEALRHDAFLRLWCAKEAVLKAHGGGIAFGLHKAVFDVGGEGLRMLRCD
                     PALGQVGDWSLHMLEPEAGFVAVLASTD"
BASE COUNT         1601 a         3807 c         3619 g         1531 t
ORIGIN      
        1 ggatcgcgtt gtcggtgtgc ttccagccgg cgttgtcggt ggcgtagccg tagtcgggct
       61 tgccgtcgaa ggtgagctgg gtctccttgc cgctggcgag gtcgcgcagc cacaggttcc
      121 agtcgcggac gaaggccgcg cggctgccat ctggcgatgc ggcgcccggc tcggtgccgg
      181 acgcgggctt cgccaacgcg gcgcagccgg ccgcgtcgca gcggtaggcg ctgccgcgca
      241 ggtcgatgcg cagggcatcg ccatcacgct tgaacccgcc cgccatcagc ttgtcgggat
      301 cggccggctt gcccttcggg ttggccttgt cgatcgccgc ggccagcgcc ttgtggtcga
      361 aggccggcgc gggcgtgccg ctggccgggt cgaagcgcag ccatgcagtc ttgccgccgg
      421 ccgacgcgcc gtagaccacg ctgccgtcgt ccagccacgc caccccgcgc acgccgccat
      481 ccaccagcgg cgcggtgcgg tcgcccagca tcttcgcggc ccgctcgtaa tcggccgcgg
      541 tgacctgcgc cgctgcgtcg cccatgccca tgccgctcac cgccagcgca cccaacagcc
      601 agtatttcgc atccatcccg atcctcgtcg cccgtccgcc gcgcggcagt cggccgatgc
      661 tagcgcgcgc ggcgcggcgg cggcgcgtga cgaacggcat ggctggcata ctggtacgga
      721 tggagaacgt cgtcaccgcc cccgccttga cgcccgagcg catcgccgtg gtcatccccg
      781 ccctcaacga ggccctgcgc atccgcgacg tggtggaagg cgcgctgcgc cactgtccga
      841 atgtcatcgt catcgacgac ggatccgacg acggcaccgc caccgcgctg gccggcctgc
      901 cggtcacggt gctccgccac gcccgccgca tgggcaaggg cgcgggcctg cgcgacggct
      961 tcgccgaagc cctgcggcgc ggcttcgacg cggtgatcgc gatggacggc gacggccagc
     1021 acagcggcga cgacatcccg cgcctgctgg ccaccggcaa ccgcttccgc ggctggatcg
     1081 tgatcggcgc gcgcctgcgc aagcgcgcca accagccgcg ccaccgccgc ctggccaacc
     1141 ggttcggcga ctggggcatc gcctggggca ccggctacca ggtcgccgac agccagagcg
     1201 ggcagcgcct gtatccggcg gacgtttgcc ggcttggctg cagcggcatg cccggcgagg
     1261 acttcgtgtt cgaggcgcag atcctgatgt ccgccgcaca gcaactgggc acgcgctgcg
     1321 tgtcggtgcc gatcgaggcg cgctacaacc aggtccacgg ggcgccggac ttccgcccca
     1381 gccactttcg cccattgcgc gacctgtggc ggatcaccag ccatatcgtg ctccagtgcg
     1441 tgcgccgcgg cggcctgtgg gaggtgcaca agcgggtgcg gcaacacccg ccggtgatcg
     1501 acgatccatc cggcgaattc gccggcgtgc aggcacggcc gcaggcccgg tgacgcggcc
     1561 gccggcgacc cgcgcgctat catccgcgcc ccgatgcacg ccctgaacga cagcctcccg
     1621 caccccgccg cggccagcgt gcgcgtcgcc taccggcacg cgccactgga catgctgctg
     1681 gccgatccct gcctgctggc ggtgttcggc ttcggcgacg cggcgcccgc gcagcacgac
     1741 gacccgcgct acctgcacgt cgccctgcag gcgctgggca cggcgccgtt cgaatgctgg
     1801 tcggtcgcag gcaacgtgcg ccacgggcgc gacgacggca ttgcctggag cgcggatgcc
     1861 accctgcagt tcggtgcgct ggaggtggcg gacgacggcg atgtcgaagc cgccagcgcg
     1921 caggcctacc agcgcctgca ggcgtggctg gcagccagtg gctacccgca tccgctgcgc
     1981 ctgtggaact acatggatgc gatcaccgag ggcgagggcg acggcgagcg ctaccgccgt
     2041 ttctgcgtgg gccgggcacg cgggatcggc cgcgaactgg cgccggccga actgcccgcc
     2101 gccaccgcga tcggcgatcc ccgccgcagc ggccgcttcc agttgtactg gctggccgcg
     2161 cgcgcggctg gcaccgcgct ggagaacccg cgccagaccc aggcctggcg ctacccgcgc
     2221 cagtacgggc cgcaggcgcc cgggttcgcc cgcgccatgc tgccggcgaa cgaatcgatg
     2281 ccgctgctgc tgtccggcac cgccgcggtg gtcgggcatg cctcgcagca cggcgattcg
     2341 ctggccgcgc aactggacga aaccctggcc aacctcgaca gcctggtcgc caccgcgcgc
     2401 gcgctgcggc ccggcctggc ggcgcggctc ggggcgggtt cgccactcaa ggtgtacgtc
     2461 cgcgatgcgg atgcgctggc gcaggtcgcg gcgctgctcg accagcggct gccttccatg
     2521 gtcccgcgcc tcgtcctgca cgggcatgtg tgccggcgcg aactggcggt cgagatcgac
     2581 ggcgtgcacg cctgagcatt gcatcgccgc gatccggcgg catagtggcg gcaacgacaa
     2641 ccggggaacc gacaatggga ctgatcagcg tgctgtgggg aatcgtcgcc atgctgtgga
     2701 tggtgctggc cttcatcccg ctgctgggct ggggcaactg gttcatgatc ccgttcgccg
     2761 tggtcggcgc gatcatcgcc gcgatcggca tcgccatcac cccgcgcgag ggtcgcggcc
     2821 gcgccaaggc cggcctggtc ctcaacgtgg tcgcgatcgt ggtcggcgcg atccggttga
     2881 gcatgggcgg cggcgtgctc tgaccgaagc ccggtggccc cgggtgccga acggcagttg
     2941 ccgcaaaacg gggttttggc tatacagcgg tcaccggtgg cggcgacagc acaccgggga
     3001 tgccgcggcc gttccggtcc gccgggcgca accgttgtcg catcaagggg atccaatcga
     3061 tgtcagcaaa caccaggaac cggtgcctgg ccgccagcct tgccatcgcc gcgcttgcgg
     3121 ccggcagcgc acaggccgcc aagcccatga acgtacttga gaagaaggcc gccgccgagg
     3181 cgatggccct cgcctaccag cagcgggccg caaccaagca ggtcgcccgc aagactgctc
     3241 cgaccaccct ggccgccccg ggcggcggca ccggcaccga ggttcctgac gacctccaca
     3301 actacctgca tgcgcaggtg gatgcccagg gcagcgtgcg catcgtcgag accgatggcc
     3361 cgaccgcgcc ggcgaagcag gaggcgaacc atgagtaagt cgccccgcct cgccctcgcc
     3421 accgccgtgc tggtcgcgct ggccggcctg tcacaggcca acgccgccga tatcgttccg
     3481 gtcatcttcg atgccgcggg aactggctac aacgacacca ccccggtcgc cccggtgggc
     3541 ggcaaccccg gcaccaccct cggtgaacag cgccgcatcg tggccaacta tgccgcctcc
     3601 ctgtggggca gcgtgctgca gagcaacgtg cctgtctacg ttggcgcgcg cttcaccgcg
     3661 ctggcaccag gcgtactggg ttctgccggc gcgaccgcag tgcacgcaaa cttcgccaat
     3721 gccccggttg ccagcacctg gtaccacacg gcactggcag actccatcgc aggcatcgac
     3781 ctgcgtcctg gcttcaccga catcagttcc aacttcagca ccgcgtaccc cttttactat
     3841 ggcctcgacg gcaagacccc gaccgggcag gtgaatttcc tggacgtggt gatgcacgag
     3901 tacgcgcatg gcctgggctt ctccaacttc gagaccgagg ccaccggtgc attcctgagt
     3961 ggagtccccg acatctattc ggcattcacc tacgacaaca ccgccggcaa gtactggacg
     4021 gcgatgaccg tagccgaacg cgccacgtcc ggcatcaaca ccggcaacgt ggtgttcacc
     4081 ggcccgcgcg cgacggcagg tgcccagttg ctgctggaca agcggcttta cttccgtgcg
     4141 tccgcgccgg ccgcggttgc aggcgactac caatacggta ccgccacgtt cggccctgcc
     4201 gcatcgccgg cgaacttcaa cggctcgctg gcgctggcca ccgacggtgt ggggaccacc
     4261 ggtgacggct gcgagccgat caccagcaac gtcgttggca aggtcgcgct gatggatcgc
     4321 ggcaactgtg gcttcgcaat caaggccaag aacgcgcaga acgcaggtgc aagcgccgtg
     4381 gtcatcgccg acaacgtggc gggcgccgtc gccggcatga gtggcgcgga tccgacgatc
     4441 accattccct cggtgcgcat caccctggcc gacggcaacc tgttcaaggc caacctgccg
     4501 gtcagcctgg gcttcgtcgc cgatccggtg ctgctgcagg gcgccgacag ccttgggcgg
     4561 ccgcgcctgt tcatgccgaa cccggtgcag gccggctcgt cgggctcgca ttacgattcg
     4621 gttgccatgc ccaacctgct gatggaaccg gcgatcaacg acagcctgcg cagctactac
     4681 aacctcgaca tcaccccggc gctgatggcc gatatcggct ggaagctgaa caccggcaac
     4741 gccaagatcg gcagcccgcg ggtcgcggat tgcgacaccg ggatcaaggc agtcagcgaa
     4801 ggcggcctga tcgtcggcgg taccgtgcag gccaacaatg acatgtgcct gctgtcggcg
     4861 cagacccgct ggggctacta cagctgcatg caggccagcc gcgaccggat gctggcggcc
     4921 agcctgatca ccaccaccca ggccagcaag atgatgacct gcgcgcggcg cgtcgccgac
     4981 aacacggcct tcccgctcta atccagggaa atcttggagc accacccgac cggcgccgca
     5041 aggcgccggt ttttttgcgc cggcgtaaac tgcgcgcatg acctacccct actcccgccc
     5101 gcggcggatg cgccgcgacg aattctcgcg ccggctgatg cgcgagaccg tgctgaccgc
     5161 gaacgacctg atctacccgg tgttcgtgca tgagctggcc ggccgcgagg cgatcgcctc
     5221 gatgcccggc atcgagcgcc tgtcgatcga cgaattgctg cgcgtggccg aacaggccag
     5281 cgagctgaag gtgccggcgc tggcgctgtt cccggtcacc gcgccggagg cgaagtcgct
     5341 ggatgcggtg gccgcatggg acgacgcggg cctgtgccag cgcgcggtgc gtgcgctcaa
     5401 gcagcgcttc cccgaactcg gcgtcatcac cgacgtggcg ctggatccgt acaccagcca
     5461 cggccaggac gggctggtcg acgacagcgg ctacgtcatg aacgacgaaa ccgtggaagc
     5521 gctggtcagg caggcgctgt cgcatgcgcg cgccggcgcg gacgtggtcg cgcccagcga
     5581 catgatggac ggccgcatcg gcgccatccg cggcgagctg gaagcagaag gcttcgtcca
     5641 cacccgcatc ctggcctaca gcgccaagta cgcctccgcg ttctacggcc cgttccgcga
     5701 cgcggtgggt tcggccggcg cactcggcaa gggcaacaag tacacctacc agatggaccc
     5761 ggccaattcg gacgaggcgc tgcgcgaaat cgcgctggac ctcgacgagg gcgccgacat
     5821 gatcatggtc aagccgggca tgccgtacct cgacatcgtg cgccgggcca aggacgagtt
     5881 cggcgcgccg accttcgtct accaggtcag cggcgagtac gcgatgctca aggccgccgc
     5941 gcacaacggc tggctggacg aacgcgcctg cgcgctggaa gcgctggcca gcatcaagcg
     6001 cgccggcgca gatggcgtgc tgacctattt cgcgctggac gccgcgcgct ggctgcgcga
     6061 gacgcactga ccgctctaca accgggggga tcgcgatgcg caagcaccac gccgtgttcg
     6121 gaacgccgat ttcgcactcg ctgtcgccgc gcatccacgc cgcgttcgcg cgccagtgcg
     6181 ggatcgaact cgactacctc gcgatcgaag ccgatgccgg cgacttccgc gcgaaactcg
     6241 atgacttcgc cgcccacggt ggcaccggcg cgaacgtcac cctgccgctg aaggaggccg
     6301 cgttcgcgct gtgcagcgag gccagcgaac gcgcacgccg tgccggcgcc gtgaataccc
     6361 tggtccgcgc cggcgacggc tggcacggcg acaacaccga cggtgccggc ctggtgcgcg
     6421 acctcaccgg ccgccacatg ctcgacctgc gcgcgcggcg caccctgctg ctgggtgccg
     6481 gcggcgcggc gcgcggcatc gcaccggcat tgctggaagc cggcatcggc gagctgacca
     6541 tcgtcaaccg cacccccgag cgcgccgacg cgctggcgga tgcgcttggc gaacccggcc
     6601 gggtgcaccc gcgctacctc ggcgaccttg ccgcacttgg cgaattcgac ctgatcgtca
     6661 acgccacctc ggccgcgcgt ggcggccagc tgccagcgct gccacggtcg ctggtcgggc
     6721 gccgctgcgc cgccgtcgac ctcagctacg gcgaggccgc gatcccgttc ctggcctggg
     6781 cacgcgcgaa cggctgccac gatgctgtcg atggcttggg catgctggtc gaacaggcgg
     6841 cggagagctt cgcgctgtgg cttggcacgc gcccggaaac cgatggcgtg tacgccgagc
     6901 tcagcggacg cgcagcggcg ctggtgaccg cggactgagt gcagcatcga gcgatggtgg
     6961 ctggcgtgcg gggccgccga gtgggttcga gcgggccatg gatggcccgc ggccgtgagc
     7021 tcagtcctgg acgactggag cgagcggcga atccactcgg cggccccgca cgccagccaa
     7081 cgcttagccc aggtattcgt ccttcaaccg cacgtaatgc ccggcgctgt aatacagcgc
     7141 ctcgatctcg gcctcgctga gcagccgcgc cttcttcgcc gggttgccca gccacagctc
     7201 gccttcgccc accaccttgc ccggcgccac cagcgcgccg gcgccgatga aggcgtgctt
     7261 cttcaccacc gcgccgtcca gcacgatcgc gcccatcccg atcagcaccg cgtcctcgat
     7321 ggtgcaggcg tggatgatcg ccttgtggcc gatggtgacg tcgctgccga tgcgggtggc
     7381 gaagccgccc agcttggcgt gcgggccgtc gtggctgacg tggatgacgg tgccgtcctg
     7441 gatattggtg cgatcgccga tgcggacgaa attcacgtcg ccgcggatca ccgtgaacgg
     7501 ccacaccgag acgtcgtcgc ccagcaccac gtcgccgatc acgcaggcgg cgggatcaac
     7561 gtaggcgcgc gcgccgaggg tcggggaaat gtcgcgataa gggcggatgt gcatggctgg
     7621 attctagccc gcagccgact acactgaccc gtatggacaa ccccgccgat tccgccacca
     7681 gcgaccttgc gcccaccctg gcccgcctgc gcgcggcctg gcagcagcgc aagccggatc
     7741 atgcccagcg ccgcggcgac ctgcagcgcc tgcgcgatgc gctgaagggc cggctcgacg
     7801 aaatggccga cgccatcgcc gcggatttcg ggcaccgctc gaaggacgag agccggatcg
     7861 ccgacggcat gaccgtgctc aacgacatcg accacctgct caagcacctg cgcggctgga
     7921 tgaagccgcg ccgggtgggc gtgggctggc gcttcctgcc ggcgcgcgcg cagctgcggc
     7981 cggtgccgct gggcgttgtc ggggtgatcg cgccgtggaa ctatccggtc aacctggcgc
     8041 tgattccgct ggccaccgcg atcgccgccg gcaaccacgt ctacctcaag ccttccgaac
     8101 acaccccgcg caccagcgaa ttcctgcgcc ggctgctggc cgaggtgttc ccggccgacc
     8161 gggtcgcggt cgcgctcggc ggggcggagg ttggcgcggc gttcgcggca ctgccgttcg
     8221 accacctgtt gttcaccggt tccaccgcgg tcggccgcaa ggtgatggcc gcggccgcgc
     8281 ccaacctgac cccgctgacc ctggagctgg gcggcaagtc gccggcgatc atcgccgacg
     8341 actacccggt cgaactggcc gccgcgcgca tcgccaccgg caagtggttc aactgcgggc
     8401 agacctgcat cgcgcccgac tacgtgctgg taccggcggc caggcgcgat gcgctggtcg
     8461 cggcgctgcg cgcgcaggtg cacgcgcgct acggcgccgc cctgtccaac ctcggcgact
     8521 acacccggat cgtcaacgac ggccagtacg cgcggctgca ggggtacctc gacgacgccc
     8581 gcgcgcgcgg cgtcgaggtg gtgaccctgg ccggtgccgc ggatgcggcg caacgcgtgc
     8641 tgccgcccac cctgctgctg gatccgggcg acgacgcccg ggtgatgcag gacgagatct
     8701 tcggcccgct gctgccgctg aagtcctacc gcacgctgga cgaggcgatc gcctacgtca
     8761 atgcgcacga ccgtccgctc gcgctgtatc ccttcagcaa ccaccgccag acggtggaga
     8821 agatcctgca tgccaccctg tccggcgggg tctcggtcaa cgacaccctg ttccacttcg
     8881 cgatcggcgg cctgccgttc ggcggggtcg gccccagcgg catgggcgcc taccacgcgc
     8941 gcgccggctt cgacgccatg agcaagcaac tgccggtgct gtggcaggcg cgctggaccg
     9001 gcggcgacct gctcaagcca ccgtattcga aggcgaagtg gctgctcgac ctgatcgtgc
     9061 gttgacgcgg gccgccagcg ccaccacatc gaataggatg cggcgatgaa gatcgccagc
     9121 tggaacgtca attcgctcaa cgtgcgcctg ccgcacctgc aacgctggct ggacgaggcg
     9181 aagccggatg tggtcgcgct gcaggaaacc aagcaggaag accacaagtt ccccgaggac
     9241 gcgttgctgg aactcggtta ccgcagcgtc ttcagcgggc agaagaccta taacggcgtg
     9301 gccatcatca gccggttgcc gttcaccggc gaatgcgtga acgcgatccc gggcttcgac
     9361 gacccgcaga agcgcgtgct ggcggcgacc gttggcgacg cacgaatcgt cgacctgtac
     9421 gtggtgaatg gcgaagcggt cggcagcggg aaattcgact acaagctgcg ctggctggcc
     9481 gcggtgcgcg agtggctgcg cgcggaaatc gccgcccatc cgaacctggt ggtgctcggc
     9541 gacttcaaca tcgcgcccga cgaccgcgac gtgttcgatc ccaggcgctg gcgcgagaag
     9601 gtcctgtgct ccagcccgga gcgcgaggcc tatcgcgccc tgctcgagct cggcctgcac
     9661 gacagcttcc gcctgttcaa cgacgaggcc gggcaccaca gctggtggga ctaccggctg
     9721 tcgggcttcg agcgcggttg gggcctgcgc atcgacctgg tgctggtcag cgaggcgctg
     9781 aaggcgcgtt gcgtcgcggc cggcatcgac cgcgagccgc gcagctggga gcggccgagc
     9841 gaccacaccc cggtctgggt ggagatcggc ccctgattgc gcaccggtcg cacgcccgcc
     9901 accacgacct tcgaccatgc tgatggacgg gacgcgggat ttttccatcg atgcgatgca
     9961 gtgccgatgg cagcacctgc cgggcaccgc gcgtgcacgg gacatcgcca tccactggct
    10021 gcgcgtcgtc gccggggatg cggcggccga cagcctgcac cgggatgcgc gcaaccggcc
    10081 gcggctcctg cacggcgaca ctagctggtc gcacagccac gcgcgcctgc tggttgccta
    10141 cacgccgcgc ggacgcgtcg gcgttgacat cgaatcgcgc gtacgcggca ccgacccgct
    10201 gcggatcgcg cggcgctact tcgcgccgga ggaaaccgcg gcactcgccg cgctcgacga
    10261 ggccctgcgg cacgacgcgt tcctgcgcct gtggtgcgcc aaggaagcgg tgctgaaggc
    10321 gcatggcggc ggcatcgcgt tcggcctgca caaggcggtg ttcgatgttg gcggcgaggg
    10381 actgcgcatg ctccgctgcg atccggcgct gggccaggtc ggggattggt cgctgcacat
    10441 gctcgagccg gaagcggggt tcgtcgccgt ccttgcatcc accgattgac cccctcgcgg
    10501 cggcggggtg gcccggcatt cgccacgcgc ctcgaaatgg cgctgcgcga ataccggg
//
LOCUS       DAORUK010000063        10550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_6618_length_10550_cov_16.774178, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000063 DAORUK010000000
VERSION     DAORUK010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10550
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6618_length_10550_cov_16.774178"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2388)
                     /gene="secA"
                     /locus_tag="PK818_05810"
     CDS             complement(<1..2388)
                     /gene="secA"
                     /locus_tag="PK818_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017112773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HQY82284.1"
                     /translation="MLNSLLTSVFGSRNERLLKQLNGIVKKINALEPQVQGLSDEALK
                     AKTEEFRGRVGKGESLDKLLPEAFAVCREASIRVFGMRHFDVQLVGGMVLHSGKIAEM
                     RTGEGKTLTATLAVYLNALEGKGVHVVTVNDYLARRDAAQMGKLYNWLGLSVGVVFPG
                     MPHSDKGAAYSCDITYGTNNEFGFDYLRDNMALAKEDRFQRGLHYAIVDEVDSILIDE
                     ARTPLIISGPADESPELYVRVNRIVPSLARQEKEDGDGDFFVDEKGKQVHLSESGQEH
                     AEALLREAGILGEDESLYAPQNIHVVHHLNAAMRAHAIYQRDVDYIVRDNEVVIVDEF
                     TGRTLPGRRWSDGLHQAVEAKEGVAVQRENQTLASVTFQNLFRMYKKLAGMTGTADTE
                     AYEFQSIYGLEVVVIPTNQPMVRKDHSDLVFLNRAGKYRAVANEIKACAGKGQPVLVG
                     TTSIEVSELLSDALRDAGIPHEVLNAKQHEREAHIVAQAGRPGAVTIATNMAGRGTDI
                     VLGGSLDAEMADLEAQGPVDDATRARLKAEWQVRHDAVKAAGGLHIVGTERHESRRID
                     NQLRGRSGRQGDPGSSRFYLSLEDNLMRIFAADWVQKVMARMGLKEDDIIESPLVTKQ
                     IANAQRKVEAHNFDIRKNLLDFDDVNNDQRKVIYGQRDELLDAESIKENVDGIRHDVV
                     GDLVQRFVPLNSIDEQWDLPGLEAELASEYGVHMALVQLHQQHEELDAEQIRDKVLDA
                     VDALFADKEAQVGGDTMRMLEKHVMLNVLDQNWKEHLGRMDYLRQGIHLRGYAQKQ"
     gene            complement(2550..3506)
                     /locus_tag="PK818_05815"
     CDS             complement(2550..3506)
                     /locus_tag="PK818_05815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQY82285.1"
                     /translation="MTETNQQRRNSIHDTRQRLVALAYRLRFHALRRPLAAVGAVLAV
                     GVLLGAGGRSVVGMAEVEALQAADAGRQAELDKVRREAQHEVNALAARLSELQAQANR
                     LNALGARLTRAGQLQDGEFEFDQPVGQGGSGRARDMPPAELRRSIALLEHQFGDADTQ
                     LSVLEALLFNRQLDRSALPSRDPIANSYITSGFGGRADPFGGGRQFHKGIDFEADVGD
                     PVLAVADGVVSYSGVRSGYGNVIEIDHGNGYVTRYAHNSALDRSVGDLVRSGQQIARA
                     GSTGRSTGAHVHFEVWQDGAVVNPRKFLGHNDALARHGPDRG"
     gene            3512..4030
                     /locus_tag="PK818_05820"
     CDS             3512..4030
                     /locus_tag="PK818_05820"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HQY82286.1"
                     /translation="MPGSRSTPPNAGTKGSTGPRAALDALFAEATGNPLRRALWLDAV
                     DQLLRPCLPPGVAAHARLANVRGDRLVFVVDAPVWHAKLRLATPELVDAARSVGLDVA
                     GLSVKTTLQPVRPLPPATRTAPPMSAAGQSQLEAALALLRSDALDSAGGDRTTGRRRT
                     RKVPTEPASGAS"
     gene            complement(4186..5103)
                     /gene="lpxC"
                     /locus_tag="PK818_05825"
     CDS             complement(4186..5103)
                     /gene="lpxC"
                     /locus_tag="PK818_05825"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HQY82287.1"
                     /translation="MLRQRTLKNLIRATGVGLHSGEKVFLTLRPAPVDTGIVFRRTDL
                     DPVVEIPANATLVSETVLCTGLSCGPAKVQTVEHLLSALAGLGIDNAYIELTAPEVPI
                     MDGSAGPFVFLLQSAGIAEQDAAKRFIRILKPVEVRDGDKRARFEPHDGFRIGFTVAF
                     NHPAIPTALSRAEVDFSTDAYIREVSRARTFGFMHDLEYMRERNLGLGGSMDNAIVLD
                     EFRVLNEDGLRYADEFVRHKILDAVGDLYLAGHAIIGAFEGFKSGHALNNTLVRALLA
                     DAGAWEEVRFTDPVATPPVLYGAAVPAVS"
     gene            complement(5323..6573)
                     /gene="ftsZ"
                     /locus_tag="PK818_05830"
     CDS             complement(5323..6573)
                     /gene="ftsZ"
                     /locus_tag="PK818_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017170527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HQY82288.1"
                     /translation="MAHFELVEQMAPNAVIKVVGVGGGGGNAVAHMVNSSVDGVEFIT
                     ANTDSQAIKNCGAKTQLQLGGNVTKGLGAGANPEVGRQAALEDREAIIDALSGADMVF
                     ITAGMGGGTGTGAAPVVAQIAKELGILTVAVVTKPFPFEGRRRMQVALKGIEELSQHC
                     DSLITIPNEKLITVLGRNATMIQAFRAANDVLLGAVQGIADLIVRPGLINVDFADVRT
                     VMSEMGLAMMGSGSARGDDRAQAAAEGAIQNPLLDDVNLSGANGILVNITAGPDFTMA
                     EFDEVGRTVEQFASEDATVVIGTVLDPDMQDEVRVTVVATGLNRASSRTALPRDRHET
                     SARFETGFDAPRRPQVQLISTQGKRDGTTGLMIDEVADPFVPNASPSFVGGLRRGADA
                     APAVADLPKDDYLDIPAFLRRQAD"
     gene            complement(6698..7933)
                     /gene="ftsA"
                     /locus_tag="PK818_05835"
     CDS             complement(6698..7933)
                     /gene="ftsA"
                     /locus_tag="PK818_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HQY82289.1"
                     /translation="MNRKGDKSLIVGLDVGTSKVTALVGEYAPGEPIEVIGIGSHESR
                     GLRRGVVVDIDSTVQSIQRAVEEAELMAGCEVRSVYASISGNHVQCRNSQGIAPIREG
                     EVTYADLDRVLEAAKAVAIPADQKILHAIPRDYVLDDSQEGIRNPVGMSGVRLEVHAH
                     LVVCAQSAAANISKCVQKCGLQVDDLVLSSLASSTAVLTGDEKELGVVLVDMGAGTTD
                     IAVFVQGAISHTASLPVAGDKVTEDIAHMLRTPTPEAEQIKVRYACALAQLARAEESI
                     QVPSVGDRPPRRLPRHALAQAVQARYEEIFEMVQAELRRSGFEQLVRAGMVLTGGASK
                     MEGVVELAEEMLQMPVRIGIPQHVTGLGEVVGNPVHATGVGLLLMGSQIENPRRSVLP
                     SGKVGGLFGRLSKWYRGEF"
     gene            complement(7930..8718)
                     /locus_tag="PK818_05840"
     CDS             complement(7930..8718)
                     /locus_tag="PK818_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HQY82290.1"
                     /translation="MLRLLGWILALALVALPVVAVVNGWVGSAQFPLRTLRVQGQLQR
                     VDEAQLRATVLPFAQRGFFAVPLDRIRDAVAVLPWVEHADVRKHWPDVLVVAISEHRP
                     FARWGDARLLSEQGRIFPARGVTVPQGLPLLDGPQSRVPEVVALYNQARERLANVGGV
                     RGVALDKRGSWSILLNNGTEVVLGRSDPEARLQRFAPLLPRLAAQHPGRRLARADLRY
                     TNGFSLSWVDLPKAEALPSPMPEAIPSHPFPAGGEGRNAQEAST"
     gene            complement(8724..9752)
                     /locus_tag="PK818_05845"
     CDS             complement(8724..9752)
                     /locus_tag="PK818_05845"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019338071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HQY82291.1"
                     /translation="MNTTAFPPLRITDPRDFGRVAVLMGGSSAEREVSLDSGRNVLEA
                     LQSRGVDAHAVDGIPALVDALVHGKFDRVFNILHGNKGGGEDGVLQGLLEGFEVPYTG
                     CGVLASALTMDKIRTKQVWLSAGLPTPKFIRLAPWADDAHGCASAAGGRMPGAAVRAA
                     ALQLGLPVFVKPSSEGSSVGVARVVDEAAIDDAIRVAQTYGGEMLMEQMVVGDELTVG
                     ILGDIALPSIRIVPKGEWYDYNAKYIADDTQYLCPGLDGAQEDEIRRIALAAFRAAGC
                     TGWGRVDVMRDRASGALTLLEVNTAPGMTSHSLVPKAARALGVEFDELCWRILEQSVP
                     PAHREAST"
     gene            complement(9749..>10550)
                     /locus_tag="PK818_05850"
     CDS             complement(9749..>10550)
                     /locus_tag="PK818_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017165008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HQY82292.1"
                     /translation="EFLHRLPFYGLAVLCIDDAEVAALAENTPRHVVTYGFAEHADVR
                     AVDVSQQGARMQFTLCLPDGSRTQVELALPGRHNVQNALAASAVAWQLGVGNEAIARA
                     LQKFAGIGRRFNQLATLPLASGGEVTLVDDYGHHPKELAAVFDAARGGWADKRLVVAF
                     QPHRYSRTRDLFDEFAAVLSGVDALVLTEVYPAGEAPIAGADAKSLARSIRTRGRVDP
                     VVVNNAGELRDALAGVLRDGDLLLLMGAGDIGAAAQQLARNGFIGETA"
BASE COUNT         1550 a         3664 c         3558 g         1778 t
ORIGIN      
        1 ctgcttctgc gcatagccgc gcaggtggat gccctggcgc aggtagtcca tccgccccag
       61 gtgctccttc cagttctggt cgagcacgtt cagcatcacg tgcttctcga gcatgcgcat
      121 ggtgtcgccg ccgacctgcg cttccttgtc cgcgaacagc gcgtccaccg cgtccagcac
      181 cttgtcccgg atctgctcgg catccagttc ctcgtgctgc tggtgcagct gcaccagcgc
      241 catgtgcacg ccgtattcgc tggcgagttc cgcttccaga cccggcaggt cccactgctc
      301 gtcgatcgag ttgagcggca cgaagcgctg caccaggtcg ccgaccacgt cgtggcggat
      361 gccgtcgacg ttttccttga tgctctcggc gtccagcaac tcgtcgcgct ggccgtagat
      421 caccttgcgc tggtcgttgt tgacgtcgtc gaagtcgagc aggttcttgc ggatgtcgaa
      481 gttgtgcgcc tcgaccttgc gctgcgcgtt ggcgatctgc ttggtcacca gcggcgactc
      541 gatgatgtcg tcttccttca ggcccatccg cgccatcacc ttctgcaccc agtcggcggc
      601 gaagatgcgc atcaggttgt cttccagcga caggtagaaa cgggatgaac ccgggtcgcc
      661 ctggcggccg gagcggccgc gcagctggtt gtcgatgcgg cggctctcgt ggcgctcggt
      721 gccgacgatg tgcaggccgc cggcggcctt gaccgcgtcg tggcgaacct gccactcggc
      781 cttcaggcgt gcgcgggtcg cgtcgtccac cgggccctgc gcctcgaggt cggccatctc
      841 ggcatccagc gagccaccca gcacgatgtc ggtgccgcgc ccggccatgt tggtggcgat
      901 ggtcaccgcg cccggccggc cggcctgcgc cacgatgtgc gcctcgcgct cgtgctgctt
      961 ggcgttcagc acctcatgcg ggatcccggc atcgcgcagc gcgtcgctga gcagttccga
     1021 tacctcgatc gaggtggtgc cgaccagcac cggctggccc ttcccggcgc aggccttgat
     1081 ctcgttggcc accgcgcggt acttgccggc gcggttgagg aacaccaggt cggaatggtc
     1141 cttgcggacc atcggctggt tggtcgggat gaccaccact tccaggccgt agatgctctg
     1201 gaactcgtag gcctcggtgt ccgcggtgcc ggtcatgccg gccagcttct tgtacatgcg
     1261 gaacaggttc tggaaggtga ccgaggccag ggtctggttc tcgcgctgca ccgccacgcc
     1321 ttccttcgcc tccaccgcct ggtgcaggcc atcggaccag cgccggcccg gcagggtgcg
     1381 accggtgaat tcgtcgacga tcaccacctc gttgtcgcgc acgatgtagt cgacatcgcg
     1441 ctggtagatc gcatgcgcgc gcatcgcggc gttgaggtgg tggaccacgt ggatgttctg
     1501 cggtgcgtac aggctctcgt cctcgccgag gatgccggcc tcgcgcagca gcgcctcggc
     1561 atgttcctgg ccggactcgg acaggtggac ctgcttgccc ttctcgtcga cgaagaagtc
     1621 gccgtcgccg tcttccttct cctgccgtgc cagcgacggc acgatccggt tgacccgcac
     1681 gtagagttcg ggcgattcgt cggcggggcc ggagatgatc agcggggtgc gcgcctcgtc
     1741 gatcaggatc gagtcgacct cgtccacgat cgcgtagtgc aggccgcgct ggaagcggtc
     1801 ttccttggcc agcgccatgt tgtcgcgcag gtagtcgaag ccgaattcgt tgttggtgcc
     1861 gtaggtgatg tcgcagctgt acgcggcgcc cttgtcggag tgcggcatgc ccgggaacac
     1921 cacgcccacg ctcaggccca gccagttgta gagcttgccc atctgcgcgg cgtcgcggcg
     1981 ggccaggtag tcgttgacgg tgaccacgtg cacgcccttg ccttccagcg cgttcaggta
     2041 caccgccagg gtcgcggtca gggtcttgcc ttcgccggtg cgcatctcgg cgatcttgcc
     2101 gctgtgcagg accatgccgc cgaccagctg cacgtcgaag tggcgcatcc cgaacacccg
     2161 gatggaggct tcgcggcaga ccgcgaacgc ctccggcaac agcttgtcca gcgattcgcc
     2221 cttgccgacg cgcccacgga actcttcggt cttggccttc agcgcctcat cggagagccc
     2281 ctgcacctgc ggttccagcg cattgatctt cttgacgatg ccgttgagtt gcttgagcag
     2341 gcgttcgttg cggctgccga agacgctggt gagcaggctg ttgagcatgg ggcggggtca
     2401 tccgtggcgg gatgcgcgcc cgaaatcggg gattcgcggc ggcatcatgg tctgggcaca
     2461 acaaaaacgg gcgccgggcg cccgttcggg agtcggatag tgtaactgga ggcagcttgc
     2521 ggggtttgca agccgcgtcc gcggcgatgt cagccgcgat ccggtccgtg ccgtgccagc
     2581 gcgtcgttgt ggcccaggaa cttgcgcgga ttgaccacgg cgccgtcctg ccagacttcg
     2641 aagtgcacat gggcgccggt ggagcgcccg gtggagccgg cccgggcgat ctgctggccg
     2701 ctgcggacca ggtcgccgac gctgcgatcc agcgccgagt tgtgcgcgta gcgggtcacg
     2761 tagccgttgc cgtggtcgat ctcgatcacg ttgccgtagc cggaacggac gccggagtag
     2821 ctgaccacgc catcggccac cgccagcacc gggtcgccga cgtcggcctc gaaatcgatg
     2881 cccttgtgga actggcggcc gccgccgaac gggtcggcgc ggccaccgaa gccggaggtg
     2941 atgtagctgt tggcgatcgg gtcgcgcgac ggcagcgcgc tgcggtccag ctggcggttg
     3001 aacagcagcg cttccagcac cgacagctgg gtgtcggcat cgccgaactg gtgctcgagc
     3061 agggcgatgc tgcggcgcag ttcggccggc ggcatgtcac gggcgcgccc ggaaccaccc
     3121 tggcccaccg gctggtcgaa ctcgaattcg ccatcctgca gctggccggc ccgggtcagg
     3181 cgcgcgccca gcgcgttcag ccggttggcc tgggcctgca gctcggacag gcgcgcggcg
     3241 agcgcgttga cctcgtgctg ggcttcgcgc cggaccttgt ccagctcggc ctggcggccg
     3301 gcatcggcgg cctgcagcgc ttccacctcg gccatcccga ccacgctgcg gccaccggcc
     3361 cccagcagca cgcccaccgc caggaccgcg ccaacggccg ccagcgggcg acgcagtgcg
     3421 tggaagcgga gcctgtaggc cagcgcgaca agccgctggc gggtatcgtg gatggaattc
     3481 cggcgttgct ggtttgtttc ggtcattgcc tatgcctggt tcgcgatcca cgcccccgaa
     3541 tgcggggacc aagggttcca cggggccccg cgccgcgctg gatgcgctgt tcgcggaggc
     3601 caccgggaat ccgttgcgcc gtgccttgtg gctcgatgca gtggaccagt tgttgcgccc
     3661 ctgcctgccg ccgggggtgg ccgcacacgc gcggctggcg aatgtgcggg gcgacaggct
     3721 tgtattcgtc gtcgatgcgc cggtctggca cgccaaattg cggctggcca ccccggagct
     3781 ggtcgacgcc gcccgatccg tcgggctcga tgtggcgggg ttgagcgtca agacgaccct
     3841 gcaaccggtg cgaccactac cgccggccac gcgcaccgca ccaccgatgt cggcggccgg
     3901 ccaatcgcaa ttggaggctg ccctggcatt actgcggtct gatgcgctgg acagcgccgg
     3961 gggagaccgc acgaccggga ggcggcggac acgcaaagtg cccactgaac cggccagcgg
     4021 ggcatcctaa cgtcacaagt ccgtgatttt gttaaataaa gcgtaatctg gaagctgaat
     4081 acgtgcgccc gttcacaggg cgacgacttg gacaggcggt ttgccacagg ggcggggcaa
     4141 gcccggctcg agggcagccc ggcgggcggg cgagcctggc cctgcttacg aaacggccgg
     4201 aacggcggct ccgtagagca ccggcggggt cgccaccggg tcggtgaagc gcacttcctc
     4261 ccaggcaccg gcatccgcca gcagcgcgcg caccagcgtg ttgttgagcg cgtggcccga
     4321 cttgaagccc tcgaaggcac cgatgatggc gtgcccggcc aggtacaggt cgccgaccgc
     4381 gtccaggatc ttgtggcgga cgaattcgtc ggcgtagcgc aggccgtcct cgttcagcac
     4441 ccggaattcg tcgagcacga tcgcgttgtc catcgacccg ccaaggccga ggttgcgctc
     4501 gcgcatgtac tcgaggtcgt gcatgaaccc gaacgtgcgc gcgcggctga cttcgcggat
     4561 gtaggcatcg gtggagaaat cgacctcggc gcgcgacagc gcggtcggga tcgccggatg
     4621 gttgaacgcg accgtgaaac cgatgcggaa accgtcgtgc ggctcgaagc gcgcgcgctt
     4681 gtcgccgtcg cgcacttcca ccggcttgag gatgcggatg aagcgcttgg ccgcgtcctg
     4741 ctcggcgatg ccggccgact gcagcaggaa cacgaacgga ccggcggagc cgtccatgat
     4801 cggcacttcc ggcgcggtca gctcgatgta ggcgttgtcg atgcccagtc ccgccagcgc
     4861 cgacagcagg tgttcgacgg tctgcacctt ggccgggccg caggacaggc cggtgcagag
     4921 cacggtctcg ctgaccaggg tggcgttggc cgggatttcg accaccgggt cgaggtcggt
     4981 gcggcggaac acgatgccgg tgtccaccgg ggccgggcgc agggtcagga acaccttctc
     5041 gccgctgtgc aggcccacgc cggtggcacg gatcaggttc ttgagggtgc gctggcgcag
     5101 catcgccgga gagtagcacg caggcggctg aacctgaacg taagaccggc ctgatcccgc
     5161 ggcgagacat ccggggacga aatgggccgg ccggcgcacg ccgaccgacc tgaaaacacg
     5221 ccgggcgggc aacccggcgc gtaaagggac atggcgttgt agccccctcc cttccgccaa
     5281 gggcggaggg gagggttggg gagggcagga ctccttcccc ggtcagtccg cctggcgacg
     5341 caggaacgcc gggatgtcca ggtaatcgtc cttcggcagg tcggccaccg cgggcgccgc
     5401 atccgcgcca cggcgcaggc cgccgacgaa gctcgggctg gcgttcggca cgaacgggtc
     5461 ggcgacctcg tcgatcatca ggccggtggt gccatcgcgc ttgccctggg tgctgatcag
     5521 ctgcacctgc gggcggcgcg gggcgtcgaa gccggtttcg aagcgggcgc tggtctcgtg
     5581 gcggtcgcgc ggcagcgcgg tgcggctgct ggcgcggttg aggccggtgg cgaccacggt
     5641 gacccggacc tcgtcctgca tgtccggatc cagcacggtg ccgatgacca cggtggcgtc
     5701 ttccgaggcg aactgctcca cggtgcggcc gacttcgtcg aactcggcca tggtgaagtc
     5761 cgggccggcg gtgatgttga ccaggatgcc gttggcgccg gacaggttga cgtcgtccag
     5821 cagcgggttc tggatcgcgc cttcggccgc cgcctgggca cggtcgtcgc cacgcgcgct
     5881 gccgctgccc atcatcgcca ggcccatttc ggacatgacg gtgcggacgt cggcgaagtc
     5941 gacgttgatc aggcccgggc gcacgatcag gtcggcgatg ccctgcacgg cgccgagcag
     6001 cacgtcgttg gccgcgcgga aggcctggat catggtcgcg ttgcggccga gcacggtgat
     6061 cagcttctcg ttcgggatgg tgatcagcga gtcgcaatgc tggctcagtt cctcgatgcc
     6121 cttcagtgcc acctgcatgc gccggcggcc ctcgaacggg aacggcttgg tgaccactgc
     6181 gacagtcagg atgccgagtt ccttggcgat ctgcgccacc accggcgcgg cgccggtgcc
     6241 ggtgccgccg cccatgccgg cggtgatgaa caccatgtcg gcgccgctca gcgcatcgat
     6301 gatcgcctcg cgatcctcca gcgcggcctg gcggccgact tccgggttgg cgcccgcgcc
     6361 aaggcccttg gtgacgttgc cgcccagctg cagctgggtc ttcgcgccgc agttcttgat
     6421 cgcctgcgag tcggtgttgg cggtgatgaa ttccacaccg tccacgctgc tgttgaccat
     6481 gtgtgccacg gcgttgccgc cgccgccgcc cacgcccacc accttgatca ccgcgttcgg
     6541 tgccatctgt tccaccagtt cgaaatgcgc catgtccgtg tcctcttgtt atgtgctgct
     6601 tgttcgagag tttgtttgcc agtctttgtt tcgccggatg tcgccacatc cgccttcgcc
     6661 ttccacccac cgcacgccgt cgcgccggcc ctgatcctca gaattcgccg cgataccact
     6721 tgctcaacct gccgaacaac ccgccgacct tgccgctcgg cagcaccgat cgccgcgggt
     6781 tctcgatctg gctgcccatc agcagcaggc cgacgccggt ggcatgcacc gggttgccga
     6841 ccacctcgcc gaggccggtg acgtgctgcg ggatgccgat ccgcaccggc atctgcagca
     6901 tctcttccgc aagctcgacc acgccttcca tcttcgaggc accaccggtc agcaccatgc
     6961 cggcgcgcac cagttgctcg aagccggagc ggcgcagttc ggcctgcacc atctcgaaga
     7021 tttcctcgta gcgcgcctgc accgcctgcg ccagcgcgtg gcgcggcagg cggcgcggcg
     7081 ggcggtcgcc gacgcttggc acctggatgc tttcctcggc gcgcgccagc tgcgccagcg
     7141 cacaggcgta gcgcaccttg atctgctcgg cttccggggt cggcgtgcgc agcatgtgcg
     7201 cgatgtcctc ggtgaccttg tcgccggcca ccggcagcga ggcggtatgg ctgatcgcgc
     7261 cctgcacgaa taccgcgatg tcggtggtgc ccgcgcccat gtccaccagc accacgccca
     7321 gttccttctc gtcgccggtc agcaccgcgg tgctcgaggc cagcgaggac agcaccaggt
     7381 cgtccacctg cagcccgcac ttctgcacgc acttgctgat gttggccgcc gccgactgcg
     7441 cgcacaccac caggtgcgca tgcacttcca ggcgcacgcc gctcatcccg accggattgc
     7501 ggatgccttc ctgcgaatcg tccagcacgt agtcgcgcgg gatcgcgtgc aggatcttct
     7561 ggtcggccgg gatcgcgacc gccttggccg cttccagcac ccggtcgagg tcggcgtagg
     7621 tgacttcgcc ttcacggatc ggcgcgatgc cctgcgagtt gcggcactgc acgtggttgc
     7681 cgctgatcga tgcatacacg ctgcgcactt cgcaaccggc catcagttcg gcttcttcca
     7741 ccgcacgctg gatcgactgc acggtggaat cgatgtccac caccacgcct cggcgcaggc
     7801 cacgcgattc atgcgacccg attccgatca cctcgatcgg ttcgcccggt gcgtactcgc
     7861 cgaccagtgc agtgaccttg gaggtgccga catcgaggcc gacgatcagc gacttgtcgc
     7921 ccttgcgatt catgtgcttg cttcctgcgc attgcgtcct tccccgcctg cagggaaggg
     7981 atgggatggg attgcttccg gcatcggact gggcagcgct tccgccttcg gcagatcgac
     8041 ccagctcaac gagaagccat tggtgtaacg cagatccgcg cgcgccagtc gccggcccgg
     8101 gtgctgtgcg gccagccgcg gcagcagcgg cgcgaagcgt tgcaggcgcg cctccggatc
     8161 gctgcggccc agcacgacct cggtgccgtt gttcagcagg atcgaccaac tgccgcgctt
     8221 gtccagcgcc acgccgcgca cgccaccgac gttcgccagc cgctcgcgcg cctggttgta
     8281 cagcgccacc acctccggca cgcgcgactg cggaccgtcc agcaggggca gaccctgcgg
     8341 caccgtgacg ccgcgggccg gaaaaatgcg cccttgttcg gacaacaggc gcgcgtcgcc
     8401 ccagcgcgcg aacgggcggt gctcgctgat cgcaacgacc agcacgtccg gccagtgctt
     8461 gcgcacatcc gcatgttcca cccagggcaa cacagcgacc gcatcgcgga tgcgatccag
     8521 cggcaccgcg aagaagccgc gctgcgcgaa cgggagcacg gtggcgcgca actgcgcctc
     8581 gtccacgcgc tgcaattgcc cctgcacgcg cagggtgcgc agcgggaact gcgcactccc
     8641 gacccagccg ttgactacgg ccaccaccgg cagcgccacc agcgccagcg cgaggatcca
     8701 gcccagcagt cgcagcatcg cgctcaagta ctcgcctcgc ggtgcgcggg cggcacgctc
     8761 tgctcgagga tccgccagca cagttcgtcg aattcgacgc cgagcgcgcg tgccgccttc
     8821 ggcaccagcg aatggctggt catgcccggc gcggtgttca cttccagcag ggtgagcgcg
     8881 ccgctggcgc gatcgcgcat cacgtcgacc cggccccaac cagtgcagcc ggcggcacgg
     8941 aacgcggcca gcgcgatgcg gcggatctcg tcctcctgcg cgccatccag gccagggcac
     9001 aggtactggg tatcgtcggc aatgtatttg gcgttgtagt cgtaccactc gcccttcggc
     9061 acgatgcgga tcgacggcag cgcgatgtcg ccgaggatgc cgacggtcaa ctcgtcgccg
     9121 accaccatct gctccatcag catctcgccg ccataggtct gcgcgacgcg gatcgcatcg
     9181 tcgatcgccg cctcgtcgac cacgcgcgcg acacccacgc tggagccttc gctggacggt
     9241 ttcacgaaca ccggcaggcc gagttgcagc gccgccgcac gcacggccgc tcccggcatc
     9301 ctgcctcccg cggcacttgc acatccatgt gcgtcgtccg cccacggtgc caggcggatg
     9361 aatttcgggg tcggcaggcc ggcgctcagc catacctgct tggtgcggat cttgtccatg
     9421 gtcagcgcac tggccagcac gccgcagccg gtatacggca cttcgaaacc ttccagcagg
     9481 ccctgcagca cgccgtcttc gccgccgccc ttgttgccgt gcaggatgtt gaacacgcga
     9541 tcgaacttgc cgtgcaccag cgcatcgacc agcgccggga tgccatcgac ggcatgcgca
     9601 tccaccccgc gcgactgcag cgcctccagc acgttgcgcc cggaatccag cgaaacctcg
     9661 cgctcggcgc tgctgccgcc catcagcacg gcgacgcggc cgaaatcgcg cgggtcggtg
     9721 atgcgcagcg gcgggaacgc agtggtgttc atgccgtttc tccgatgaag ccgttgcgcg
     9781 cgagttgctg tgcggccgcg ccgatgtcgc ccgcgcccat cagcagcaac aggtcgccgt
     9841 cgcgcagcac gccggccagt gcgtcgcgca attcgcctgc gttgttgacc accaccggat
     9901 cgacgcggcc gcgggtgcgg atcgaacgcg ccagcgactt ggcatctgca ccggcgatcg
     9961 gcgcttcgcc ggccggatac acctcggtca gcaccagcgc atcgacgccg gacagcaccg
    10021 cggcgaattc atcgaacaag tcgcgggtgc gcgaatagcg atgcggctgg aacgccacca
    10081 ccaggcgctt gtcggcccag ccaccgcgcg cggcatcgaa caccgcggcc agttccttcg
    10141 ggtgatggcc atagtcgtcg accagggtca cttcgccgcc acttgccagc ggcagcgtcg
    10201 ccaactggtt gaagcggcgg ccgatgccgg cgaacttctg cagcgcacgg gcgatcgctt
    10261 cgttgccaac gccgagttgc cacgcaaccg cggatgccgc gagcgcgttc tgcacgttgt
    10321 ggcggccggg cagcgccagc tccacctgcg tgcgcgagcc gtccggcagg cacagggtga
    10381 actgcatgcg cgcgccctgc tgcgacacgt ccactgcgcg cacatcggcg tgctcggcga
    10441 agccgtaggt gaccacgtgg cgcggggtgt tttcggcgag tgcggcgact tcggcatcgt
    10501 cgatgcacag caccgccagc ccgtagaacg gcagccggtg caggaattcg
//
LOCUS       DAORUK010000064        10477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_6690_length_10477_cov_19.021301, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000064 DAORUK010000000
VERSION     DAORUK010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10477
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6690_length_10477_cov_19.021301"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PK818_05855"
     CDS             <1..380
                     /locus_tag="PK818_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HQY82293.1"
                     /translation="MARDISHAVRGGRIPRWAEPLVRFSGLLPIAAIKPDGRLGVTGG
                     LFARKRAPEAFARYVARRARGANGWRLIVGHSDVLDDGQCLLAALRERLPVVEAHLVE
                     VGPAVGAHAGPGTLLVGLQPAPV"
     gene            389..1039
                     /gene="plsY"
                     /locus_tag="PK818_05860"
     CDS             389..1039
                     /gene="plsY"
                     /locus_tag="PK818_05860"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HQY82294.1"
                     /translation="MSPHLLTASLLLSAYLLGSVSGSLLLGRLRGVDIRQHGSGNAGG
                     TNAFRTLGWKFALAVAAFDIGKGVLATWLALRLAPVGQPLDAAAHGYLAATAAILGHV
                     WPLWHGFRGGKGAATAVGGLLVLWPLAVPPLLLAWVLVLTATGYVGLATVVAGACLPL
                     LAWWSDAEPARLWFCIGIGLYLLFTHRGNLQRLREGTESRFERARLLHRLVRGQQR"
     gene            1036..2025
                     /gene="birA"
                     /locus_tag="PK818_05865"
     CDS             1036..2025
                     /gene="birA"
                     /locus_tag="PK818_05865"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HQY82295.1"
                     /translation="MSDAAAERALLQRLAAGPVGGDVLAREAGLTRAAVWKRVAALRA
                     AGIAIAASPGRGYQLEQPLDLLDAAAIRVALAEPALSQLAALELAWSLDSTNSELLRR
                     EPPARGCAVLLAERQTGGRGRRGRAWASPLGAHVYLSLARRFEGGLGRLGGLSLVAGI
                     AACEALHALGHAGVGLKWPNDLVVADGRGLRKLGGLLVEGGGEAAGAARAVIGLGLNV
                     RMPAHSTAGIDQPWTDLASISPSPPSRNAVVAILLSHLLPALQQFDREGLAPFLPRYA
                     ALDALAGRALQVHAGTDGWPATALGIAGDGALRVRDGDGRERHVHAGDVSVRA"
     gene            2022..2792
                     /locus_tag="PK818_05870"
     CDS             2022..2792
                     /locus_tag="PK818_05870"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HQY82296.1"
                     /translation="MSTWLFDLGNTRLKFAELRDHAAPGETHALAHDDGERWLDGLPR
                     GATACIASVAAGERRVALLDALCTRFTRLHLVRTQPRCAGLRIAYADPRHLGVDRFLA
                     LLAARDAGDVLLVGVGTALTIDLLDADGLHRGGRIAPSPMLMRKALHARAPVLPDTGG
                     SYAEFADDTDHALASGCEGAALALVERSLDRAQRLLGAAPALWLHGGGADALRERLPP
                     HLWVPDVVLRGLARWQAHAFAAAPPRPAPAGGPLTLHA"
     gene            2789..3493
                     /locus_tag="PK818_05875"
     CDS             2789..3493
                     /locus_tag="PK818_05875"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQY82297.1"
                     /translation="MILRAAIVFLLVINLGVGAWWMGGGNGTRRPAASATPAGVPGLR
                     LVDEAAPAAPPAAPSATAGAPASAPATAAAPAPVAAPAAPVEQCLRFGPFADDAARDA
                     ARTALAAAGVEAQPRQTPARPARGWKVHVPAFATREEANAMGEKIKAAGIADWYVMNA
                     GGDANSIALGRYASEDAARRRESELTSKGIPAQAEPLGDTAAQAWLEARLPAAADRAA
                     LAAIAPAKAVDCAPRR"
     gene            3546..3621
                     /locus_tag="PK818_05880"
     tRNA            3546..3621
                     /locus_tag="PK818_05880"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3579..3581,aa:Thr,seq:tgt)
     gene            3637..4560
                     /gene="rocF"
                     /locus_tag="PK818_05885"
     CDS             3637..4560
                     /gene="rocF"
                     /locus_tag="PK818_05885"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005989327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HQY82298.1"
                     /translation="MTRSYPPVSVFGVPTDIGASRRGASMGPEALRVAGLVEAIAARG
                     IEVHDLGDVQGPRNPARPPVEGYRHLDEVVAWNRAVFDASTRELQAGRIPIMLGGDHC
                     LGIGAIAAVAAHCRATGKKLRVLWLDAHADFNTREITPSGNIHGMPVACLCGLGPEPL
                     TAMAGFTPAITADQVRQVGIRSVDPGEKRLVQEHGLDIYDMRYIDEVGMRRAIEEALD
                     GVDADTHLHVSFDVDMLDPAIAPGTGTRVPGGVNYREAQLIMEMVADTGRMGSLDLVE
                     VNPALDKRNATARLAVDLVESLFGKSTLMRD"
     gene            4656..4787
                     /locus_tag="PK818_05890"
     CDS             4656..4787
                     /locus_tag="PK818_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HQY82299.1"
                     /translation="MKRMTLLMLLAATAVLALAGCNTIEGAGKDIKKAGEAIEGAAK"
     gene            complement(4843..5652)
                     /locus_tag="PK818_05895"
     CDS             complement(4843..5652)
                     /locus_tag="PK818_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82300.1"
                     /translation="MFRRLLLVLALASTTACAPLSARDLVEVDVIDRDNGQWLPEYRH
                     RGDSWIAGVPGHRYSVRLTNTTGQRVLVVLSVDGVNAVSGQTAAPSQAGYVLAPWESA
                     EIAGWRKSMDDVAQFLFTDLPDSYAARTGRPADVGVIGIAVFRERVQRQQYAPPLSPP
                     VAAQADAARERRAGNAAKAAAPAAAAADSAMAEAAPQRLGTGHGQREWAPVGQTEFVR
                     ASARPQQVTQLRYDDADSLVALGVLPRPHASYARATPNAFPDGFVADPPRW"
     gene            5793..7127
                     /locus_tag="PK818_05900"
     CDS             5793..7127
                     /locus_tag="PK818_05900"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HQY82301.1"
                     /translation="MSKSRVLTGITTSGTPHLGNYVGAIRPAIAASHAPGVESFYFLA
                     DYHSLIKVQDPARVQRSTLEIAAAWLACGLAPDKVWFYRQSDIPEIPELTWLLTCVAG
                     KGLLNRAHAYKAAVDRNRAEGEDDDAAISAGLFMYPVLMAADILLFNAHKVPVGRDQV
                     QHIEMARDFGQRFNHLYGEPASRAYFTLPEAVVEEQVATLPGLDGRKMSKSYDNTIPL
                     FAPRGELKKLVAGILTDSRAPGEAKDTEGSALFQLYQAFASQDETAAFARAFADGIGW
                     GDAKQALFERIDAEVAPMREAYEALMAKPGEIEAILRDGAARLRVRHATPMLQRLREA
                     VGLRDLSQVPVANARPAKKDAAAATPVFKQYRDADGKFYFKLMQGERVLLQSTGFASP
                     RDAGQRIAAIKQGEIGDGASEFSLGDGVTDEEVNAALAAFVAAQLDGESGKA"
     gene            complement(7182..8519)
                     /locus_tag="PK818_05905"
     CDS             complement(7182..8519)
                     /locus_tag="PK818_05905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HQY82302.1"
                     /translation="MSKKFLLPDLGEGLPDATIVEWYVKEGDVIRLDENLVSMETAKA
                     VVDVPSPVSGKVLKLAGGAGDVIVTGTMLAEFEIDPSLPQRAEGQDTGHHHGPSHAAP
                     EPAAAAPAPAAAAKAEPEDAGTVVGAMQVGNAVVAEAAVAVGGVKAVPAVRATARKLG
                     VDIARVRASGPDGTVTMDDVKRAAADGSAKPGAAPAARAAAPAPAAPAAAPAQRSTLS
                     QTGKPMRTQPPGAAASGQPEQLKGVRRNMARVMADARDRVVQTTLVDDADLHQWLGKQ
                     DITARTIRAIVTACRAEPALNAWFDDENLTLTKHPHVDLGLAVDTPDGLFVPALRNAH
                     LLDNRGLREGINRLRTQVEDRSIPASELSGYTISLSNFGVFAGRYGTVVVVPPCVAIV
                     GVGKLSHDVVAVMGGIEVHRRMPISLTFDHRACTGGEAARFLAALLDDLAQPT"
     gene            complement(8530..9531)
                     /locus_tag="PK818_05910"
     CDS             complement(8530..9531)
                     /locus_tag="PK818_05910"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HQY82303.1"
                     /translation="MSAQPITPAPITLIEAITQALAYELRTDESVVVLGEDVGVNGGV
                     FRATAGLSAVFGTERVLDTPLDETTIAGLTVGMASQGMKPVAEAQFDGFMYPMVDFIV
                     CHAARMRYRTRGRLTCPMVLRVPWGGGIRAPEHHSEANESIFTNVPGLRVVMPSSPQR
                     AYGLLLAAIRDPDPVIYMEPKRIYRQYKELVPDDGEALPLDVCFVLRDGSDITLVTWG
                     AQVKETLEAAEKLAAEGISAEVIDVATLTPLDFPTIAESVAKTGRCVIVHEAAKTAGF
                     GAEIAARVAEECLFDLLAPVERVTGYDTHIPLFRLEMKYLPSVDKIVAAARRAMAYS"
     gene            complement(9528..>10477)
                     /locus_tag="PK818_05915"
     CDS             complement(9528..>10477)
                     /locus_tag="PK818_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016850583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HQY82304.1"
                     /translation="FLRTFDSKAIALQRTGKLGTYAACLGHEATHVGIGASMQPEDVF
                     APSYREYGAQFARGMRPREVLMYWGGDERGNLLTGAPAHDYPWCVPISTQMLMASGAA
                     LSFKLRKQDRVAVAVCGDGGSSKTDFYAAVNSAGAYQLPLVLCVVNNGWAISVPRKAQ
                     TGAETLAQKGLAGGLHCLQVDGNDLVAVLEAMRRAHERARKGEGGSVIEFMTYRLHDH
                     TTADDARRYRGEDEVKDAWLREPFLRLRKFLTDAGLWDEAQEKAWIEESGKRVDVEIN
                     AYLETPVQPVEAMFDYLYGDMPPDLLAQRDAAIALEGRA"
BASE COUNT         1382 a         3715 c         3861 g         1519 t
ORIGIN      
        1 cgatggcgcg cgacatctcg catgcggtgc gcggcggccg catcccgcgc tgggcggaac
       61 cgctggtgcg cttcagcggg ctgctcccga tcgcggcgat caagccggac gggcggctgg
      121 gggtcaccgg tggcctgttc gcgcgcaagc gtgcgccgga ggcgttcgcg cgctacgtcg
      181 cccggcgtgc gcggggcgcg aacgggtggc ggctgatcgt cggccacagc gacgtgctgg
      241 acgacggcca gtgcctgctg gcggcgctgc gggaacgcct gccggtggtg gaggcgcacc
      301 tggtcgaggt cgggcccgcg gtcggcgcgc atgccgggcc gggaaccctg ctcgtggggc
      361 tgcaacccgc gccggtatga tccccggcat gtcgccgcac ctcctgaccg catcgctgct
      421 gctgtccgcc tacctgcttg gctcggtctc cggcagcctg ctgctggggc gcctgcgcgg
      481 ggtcgacatc cgccagcatg gcagcggcaa tgccggcggc accaatgcat tccggaccct
      541 gggctggaag ttcgcactgg cggtggccgc gttcgacatc ggcaagggcg tgctggcgac
      601 ctggcttgcg ctgcggcttg cgccagttgg gcagccgctg gacgcggccg cgcatggcta
      661 cctggccgcg accgccgcga tcctgggcca tgtctggccg ctctggcacg gcttccgcgg
      721 cggcaagggc gcggccaccg cggttggcgg cctgctggtg ctgtggccat tggcggtccc
      781 gccgctgctg ctggcgtggg tcctggtgct gaccgcgacc ggctatgtcg gcctggccac
      841 ggtggtcgcc ggtgcctgcc tgccgctgct ggcctggtgg agcgatgccg aacccgcgcg
      901 gctgtggttc tgcatcggca tcggcctgta cctgctgttc acccatcgcg gcaacctgca
      961 gcggctgcgc gaaggcaccg aatcgcggtt cgagcgcgcg cgcctgctgc acaggctggt
     1021 tcgcgggcag cagcggtgag cgatgcggcc gccgagcgcg ccctgctgca gcggttggcc
     1081 gcgggcccgg tcggcggcga cgtgctcgcg cgcgaagccg gactgacccg cgccgcggtg
     1141 tggaagcggg tcgcggccct gcgggccgcc ggcatcgcga tcgcggcatc gccggggcgt
     1201 ggctaccagc tcgagcagcc gctcgacctg ctcgacgcgg cggcgatccg cgtcgcgctg
     1261 gcggaacccg cgctttcgca actggcggcg ctggagctgg cgtggtcgct ggattcgacc
     1321 aacagcgaac tgctgcggcg cgagccgccg gcgcgtggct gcgcggtgct gctggcggag
     1381 cgccagaccg gcggccgcgg gcgccgcggg cgggcatggg cctcgccgct gggcgcgcac
     1441 gtctacctgt cgctggcgcg tcgtttcgag ggcgggctgg ggcggctggg cggattgagc
     1501 ctggtcgccg ggatcgccgc ctgcgaggcg ctgcacgcac tcggccacgc cggggtcggc
     1561 ctgaaatggc cgaacgacct ggtggttgcc gatggccgcg gcctgcgcaa gctcggtggc
     1621 ctgctggtcg aaggcggcgg cgaagccgcg ggcgcggcgc gcgcggtgat cgggctgggc
     1681 ctcaacgtgc gcatgcccgc gcattccacc gccggcatcg accagccgtg gacagacctg
     1741 gcgtcgatct cgccgtcgcc gccgtcgcgc aatgcggtcg tggcgatcct gctgtcgcac
     1801 ctgctgccgg ccctgcagca gttcgaccgc gaaggcctgg cgccgttcct gccgcgctac
     1861 gcggcgctgg atgcgctggc cgggcgcgcg ctgcaggtgc acgcggggac ggatgggtgg
     1921 ccggcgaccg cgctcggcat cgccggggat ggcgccctgc gggtgcgcga cggcgatggc
     1981 cgcgaacgcc acgtgcatgc cggtgacgtg agcgtgcgcg catgagcacg tggctgttcg
     2041 acctcggcaa cacccgcctg aagttcgccg aattgcgcga ccacgccgca cccggcgaga
     2101 cccatgcgct ggcccatgac gatggcgaac gctggctgga cggcctgccg cggggcgcca
     2161 ccgcctgcat tgccagcgtg gccgcgggcg agcggcgggt ggcgctgctg gacgccttgt
     2221 gcacgcgctt cacccgcctg cacctggtgc gcacgcagcc gcgctgcgcc ggcttgcgca
     2281 tcgcctatgc ggacccgcgc cacctcgggg tggatcgctt cctcgcgttg ctggccgcgc
     2341 gcgacgccgg cgacgtgctg ctggtcgggg ttggcaccgc gctcaccatc gacctgctgg
     2401 atgccgatgg cctgcatcgc ggcggccgca tcgcgccttc gccgatgctg atgcgcaagg
     2461 ccctgcacgc gcgcgcgccg gtgttgccgg acaccggtgg cagctacgcc gaattcgccg
     2521 acgacaccga ccacgcgctg gcctcgggct gcgaaggcgc tgcgctggcg ctggtcgaac
     2581 gcagcctgga tcgcgcgcag cggctgcttg gcgcggcgcc ggcgctatgg ctgcacggcg
     2641 gtggcgcgga tgcgctgcgc gagcgcctgc cgccgcacct gtgggtgccg gacgtggtgt
     2701 tgcgcgggct tgcgcgctgg caggcgcatg ccttcgcggc ggcaccaccg cggccggcgc
     2761 cggccggcgg cccgcttaca ctgcacgcat gatcctgcgc gccgccatcg tgttcctgct
     2821 ggtcatcaac ctcggcgtcg gcgcgtggtg gatgggtggc ggcaatggca cgcgcaggcc
     2881 tgccgcaagc gcaacgccgg cgggcgtccc gggcttgcgc ctggtcgatg aggccgcgcc
     2941 cgccgcaccg cccgccgcgc catccgccac ggctggcgcg ccggcgtcgg cgccggcaac
     3001 cgcggcggcg ccggcgccgg tggcggcgcc cgctgcgccg gtcgaacagt gcctgcgttt
     3061 cggcccgttc gcggatgacg ccgcacgcga tgccgcgcgc accgcgctgg cggcggcggg
     3121 cgtcgaggcg cagccgcggc agaccccggc gcgtcccgcg cgcggctgga aggtgcatgt
     3181 cccggccttc gccacccgcg aggaggccaa tgcgatgggc gagaagatca aggccgccgg
     3241 catcgccgac tggtacgtga tgaacgcggg cggcgatgcc aacagcatcg cgctggggcg
     3301 ctatgccagc gaggatgccg cgcgccgccg cgagtccgag ctcacctcca agggcatccc
     3361 cgcgcaggcc gagccgctgg gcgataccgc cgcgcaagcg tggctggagg cgcgcctgcc
     3421 tgcggcggcg gatcgcgccg cgctggccgc gatcgcaccg gcgaaagcgg tcgactgcgc
     3481 gccgcgccgc tagcgcggcc ccggccttgg cgcgcatgcg cgcagggtag aatggccgcc
     3541 cgcacgccgc tttagctcag caggtagagc aaccgccttg taagcggtag gtcatccgtt
     3601 cgattcggat aagcggcacc atctcgagac ctgccgatga cccgcagcta cccgcccgta
     3661 tccgtgttcg gcgtgcccac cgacatcggc gcctcccgcc gcggtgcctc gatggggccg
     3721 gaagccctgc gcgttgcggg actggtggag gcgatcgccg cgcgcggcat cgaggtccac
     3781 gacctcggcg acgtgcaggg accgcgcaac ccggcccgcc cgccggtcga gggctaccgg
     3841 cacctggacg aagtggtggc ctggaaccgc gcggtgttcg atgccagcac ccgcgaactg
     3901 caggccgggc ggatcccgat catgctcggc ggcgaccact gcctggggat cggcgcgatc
     3961 gccgcggtgg ccgcgcattg ccgcgccacc ggcaagaagc tgcgggtgct gtggctggat
     4021 gcgcacgcgg acttcaacac ccgcgagatc accccgtccg gcaacatcca cggcatgccg
     4081 gtggcctgcc tgtgcgggct gggcccggag ccgctgaccg cgatggccgg gttcaccccc
     4141 gcgatcaccg ccgaccaggt gcgccaggtc ggcatccgct cggtggatcc gggcgagaag
     4201 cggctggtgc aggaacacgg gctggacatc tacgacatgc gctacatcga cgaggtcggc
     4261 atgcgccggg cgatcgagga agcgctggac ggcgtggatg ccgataccca cctgcacgtg
     4321 agcttcgacg tcgacatgct ggatcccgcg atcgccccag gcaccggtac ccgcgtgccc
     4381 ggtggcgtca actaccgcga agcgcagctg atcatggaga tggtggccga caccggccgc
     4441 atgggctcgc tggacctggt cgaggtcaac ccggcgctgg acaagcgcaa cgccaccgcg
     4501 cggctggcgg tcgacctggt cgagagcctg ttcggcaagt ccaccctgat gcgcgactga
     4561 acgccgccgc cgcgggtttg caccgcggcg caggcgctgg ctaccctagg cgcaagcagg
     4621 gcacgcccgg tgccccgcgc caaccggagg aaaccatgaa gcgcatgacg ctcctgatgc
     4681 tgctggcggc gaccgccgtg cttgcgctgg caggctgcaa caccatcgaa ggcgccggca
     4741 aggacatcaa gaaggcgggc gaggcgatcg aaggcgctgc caagtaggcc ccggcgcgga
     4801 tgacgaatgg ccggcggatg ccggccattc gcgttcgtgg gcctaccagc gtggcggatc
     4861 cgccacgaac ccgtccggga acgcgttcgg ggtggcgcgc gcatatgacg catgcggccg
     4921 cggcagcacc cccagcgcga ccagcgagtc ggcatcgtcg tagcgcaact gggtcacctg
     4981 ctgcgggcgc gcgctggcgc gcacgaattc ggtctggccg accggcgccc attcgcgctg
     5041 gccgtggccg gtgcccagcc gttgcggcgc ggcttcggcc atcgcgctgt cggcggcggc
     5101 ggcggccgga gctgcggcct tggcggcatt gccggcgcgg cgctcgcgcg cggcatccgc
     5161 ctgcgcggcg accggcggcg acagcggcgg cgcatattgc tggcgctgca cccgctcacg
     5221 gaagaccgcg atgccgatca cgccgacatc ggccgggcgc ccggtgcgcg cggcatagct
     5281 gtccggcagg tcggtgaaca ggaactgggc gacgtcgtcc atcgacttgc gccagccggc
     5341 gatctccgcg ctctcccacg gcgccagcac gtagcctgcc tgcgacggcg cggcggtctg
     5401 gccactgacc gcgttgacgc catcgaccga gagcaccacc agcacgcgct ggccggtggt
     5461 gttggtcagg cgcaccgaat agcgatgccc gggcaccccg gcgatccagc tgtcgccgcg
     5521 atggcggtat tcgggcagcc actggccgtt gtcgcggtcg atgacgtcga cctcgaccag
     5581 gtcgcgggcg gacagcggcg cgcaggccgt ggtggaggca agggcaagca cgagcaagag
     5641 gcggcggaac atggcaggtc tcccggttgg gtggggtgcc gcgtgctgcg gcgggtgcct
     5701 gcatgcgaac gcgcggtgcc ggcaaacggg gtcgacgacg tcgccagtta gaatgcgtgc
     5761 cgaccccgca tcgcacccaa gccgcccttc gcatgagcaa gtcccgcgtc ctcaccggca
     5821 tcaccacctc cggtaccccg cacctcggca actacgtcgg cgcgatccgg ccggcgatcg
     5881 ccgcctcgca tgcgccgggg gtggagagct tctacttcct cgccgactac cattcgctga
     5941 tcaaggtgca ggaccccgcg cgggtgcaac gcagcaccct ggagatcgcc gcggcgtggc
     6001 tggcctgcgg gctggcgccg gacaaggtgt ggttctaccg gcagtcggac atccccgaga
     6061 tccccgagct cacctggctg ttgacctgcg tggccggcaa gggcctgctc aaccgcgcgc
     6121 atgcctacaa ggcggcggtc gaccgcaacc gcgccgaggg cgaggacgac gatgccgcga
     6181 tcagcgccgg cctgttcatg tacccggtgc tgatggccgc ggacatcctc ctgttcaacg
     6241 cgcacaaggt gccggtcggc cgcgaccagg tgcagcacat cgagatggcg cgcgacttcg
     6301 gccagcgctt caaccacctg tatggcgagc cggcttcacg ggcgtatttc accctgcccg
     6361 aagcggtggt cgaggagcag gtggcgacgc tgcccggcct cgacggccgc aagatgagca
     6421 agagctacga caacaccatc ccgttgttcg ccccgcgcgg ggaactgaag aagctggtcg
     6481 ccggcatcct caccgattcg cgcgcgcccg gcgaagccaa ggacaccgag ggctcggcgc
     6541 tgttccagtt gtaccaggcg ttcgcctcgc aggatgaaac cgcggcgttc gcgcgggcgt
     6601 tcgccgacgg catcggctgg ggcgatgcca agcaggcgtt gttcgagcgc atcgatgccg
     6661 aagtcgcgcc gatgcgcgag gcgtacgagg ccctgatggc gaaacccggc gagatcgagg
     6721 cgatcctgcg cgacggcgcc gcgcgcctgc gcgtgcggca cgccaccccg atgctgcagc
     6781 gcctgcgcga ggcggtgggc ctgcgcgacc tgtcgcaggt gccggtggcc aacgccaggc
     6841 cggcgaagaa ggacgccgcg gccgccaccc cggtgttcaa gcaataccgc gacgccgacg
     6901 gcaagttcta cttcaagttg atgcagggcg aacgcgtgct gctgcagagc accggcttcg
     6961 cctcgccgcg cgatgcaggc cagcgcatcg ccgcgatcaa gcagggcgag atcggcgatg
     7021 gcgccagcga gttcagcctc ggcgacggcg tgaccgacga agaggtgaac gccgcgctgg
     7081 ccgcgttcgt ggccgcgcag ctggacggcg aatccggaaa ggcctgagcg gcccgcccgt
     7141 cgccgcgggt gcgttcctcc gcacccgcac ccacggaccg gtcaggtcgg ctgcgcgagg
     7201 tcgtccagca acgccgccag gaagcgcgcg gcctcgccgc cggtgcaggc gcggtggtcg
     7261 aaggtcaggc tgatcggcat gcggcggtgc acctcgatgc cgcccatcac cgccaccacg
     7321 tcgtgggaca gcttgcccac gccgacgatg gccacgcacg gcggtaccac caccacggtg
     7381 ccgtagcggc cggcgaacac gccgaagttt gatagcgaga tggtgtagcc gctcagttcc
     7441 gatgccggga tggagcggtc ctccacctgc gtgcgcaggc ggttgatgcc ttcgcgcagg
     7501 ccgcggttgt ccagcaggtg ggcattgcgc agcgccggca cgaacaggcc gtcgggggtg
     7561 tccaccgcca gcccgaggtc cacgtgcgga tgcttggtga gggtgaggtt ttcgtcgtcg
     7621 aaccaggcgt tgagcgcggg ctcggcccgg caggcggtca cgatcgcgcg gatggtgcgc
     7681 gcggtgatgt cctgcttgcc aagccactgg tgcaggtcgg catcgtcgac gagggtggtc
     7741 tgcaccaccc ggtcgcgggc gtcggccatc acccgcgcca tgttgcggcg cacgcccttg
     7801 agctgctcgg gctggccgct ggcggccgcg cccggtggct gggtgcgcat cggcttgccg
     7861 gtctgcgaca gcgtgctgcg ctgggcgggg gcagcggccg gcgcggccgg tgccggtgcc
     7921 gcggcgcgcg cggccggtgc ggcgcccggc tttgccgagc cgtccgccgc cgcgcgcttc
     7981 acgtcgtcca tcgtcaccgt gccatcgggg ccgctggcac gcacgcgggc gatgtcgacc
     8041 cccagcttgc gtgcggtcgc gcgcaccgcc ggtaccgcct tgacgccgcc aacggccacc
     8101 gccgcctcgg caaccacggc gttgccgacc tgcatcgcgc cgaccacggt gccggcatct
     8161 tccggttcgg ccttcgccgc ggcggccggg gcgggtgccg ccgctgccgg ttccggcgcg
     8221 gcgtgcgacg ggccgtggtg gtggccggtg tcctggccct ccgcgcgctg cggcaggctg
     8281 gggtcgatct cgaattcggc cagcatggtg ccggtgacga tcacgtcgcc ggcaccgccg
     8341 gccagcttca gcaccttgcc ggacaccggc gagggcacgt ccaccaccgc cttggcggtt
     8401 tccatcgaaa ccaggttctc gtcgaggcgg atcacgtcgc cttccttgac gtaccactcg
     8461 acgatggtcg cgtccggcag gccttcgccg aggtcgggca ggaggaactt cttgctcatg
     8521 gggcgtcctt tacgaatagg ccatcgcgcg cctggcggcg gcgacgatct tgtccacgct
     8581 cggcaggtac ttcatctcga ggcggaacag cgggatgtgg gtgtcgtagc cggtgacgcg
     8641 ctcgaccggc gccagcaggt cgaacaggca ttcctcggcg acgcgggcgg cgatctcggc
     8701 accgaagccc gcggtcttcg ccgcctcgtg cacgatcacg cagcggccgg tcttggccac
     8761 cgattcggcg atggtgggga agtccagcgg ggtcagcgtg gcgacgtcga tgacctccgc
     8821 gctgatgccc tccgcggcca gtttctcggc ggcttccagg gtttccttca cctgcgcgcc
     8881 ccaggtcacc agggtgatgt cgctgccgtc gcgcagcacg aagcacacgt ccagcggcag
     8941 cgcctcgcca tcgtccggca ccagttcctt gtactgccgg tagatgcgct tgggctccat
     9001 gtagatcacc ggatccgggt cgcggatcgc ggccagcagc aggccgtagg cgcgctgcgg
     9061 cgaagacggc atcaccaccc gcaggccggg cacgttggtg aagatcgatt cgttggcttc
     9121 gctgtggtgt tccggcgcgc ggatgccgcc gccccacggt acccgcagca ccatcgggca
     9181 ggtcagccgg ccgcgggtgc gataacgcat gcgcgcggcg tggcagacga tgaagtcgac
     9241 catcgggtac atgaagccgt cgaactgcgc ctcggcgacc ggcttcatgc cctgcgaagc
     9301 catgccgacg gtgaggccgg cgatggtggt ttcgtccagc ggcgtgtcca gcacgcgctc
     9361 ggtgccgaac accgccgaca gcccggcggt ggcgcggaac acgccgccgt tgacgccgac
     9421 gtcttcgccc agcaccacga ccgactcgtc cgtgcgcagt tcgtacgcca gcgcctgggt
     9481 gatcgcctcg atgagcgtga tgggagccgg ggtgatcggt tgcgcgctca tgcccggccc
     9541 tccagcgcga tcgcggcatc ccgctgcgcc agcagatcgg gtggcatgtc gccgtagagg
     9601 taatcgaaca tcgcttccac cggctgcacc ggggtttcca ggtaggcgtt gatttccacg
     9661 tccacgcgct tgccgctctc ctcgatccag gccttctcct gcgcttcgtc ccacagcccg
     9721 gcatcggtca ggaacttgcg caggcgcagg aacggctccc gcagccaggc gtccttcact
     9781 tcgtcctcgc cacggtagcg gcgggcgtcg tcggcggtgg tgtggtcgtg caggcggtag
     9841 gtcatgaatt cgatcacgct gccgccttcg cccttgcgcg cgcgctcgtg ggcgcggcgc
     9901 atcgcttcca gcaccgccac caggtcgttg ccgtccacct gcaggcagtg caggccgccg
     9961 gccaggccct tctgcgccag cgtttcggcg ccggtctggg ccttgcgcgg cacgctgatc
    10021 gcccagccgt tgttgaccac gcacagcacc agcggcagct ggtaggcgcc tgcggaattc
    10081 accgccgcgt agaaatcggt cttcgaggag ccgccatcgc cacagaccgc caccgccacc
    10141 cggtcctgct tgcgcagctt gaagctcagc gcggcgccgg aggccatcag catctgggtg
    10201 gaaatcggca cgcaccacgg atagtcgtgc gccggtgcgc cggtcagcag gttgccgcgc
    10261 tcgtcgccgc cccagtacat cagcacctcg cgcgggcgca tgccgcgggc gaactgcgcg
    10321 ccgtattcgc ggtagctggg tgcgaacacg tcctcgggct gcatcgacgc gccgatgccg
    10381 acgtgggtgg cctcgtggcc caggcaggcg gcataggtgc cgagcttgcc ggtgcgctgc
    10441 agggcgatcg ccttgctgtc gaaggtgcgc aggaaca
//
LOCUS       DAORUK010000065        10474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_6694_length_10474_cov_17.616182, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000065 DAORUK010000000
VERSION     DAORUK010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10474
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6694_length_10474_cov_17.616182"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="PK818_05920"
     CDS             complement(<1..1421)
                     /locus_tag="PK818_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HQY82305.1"
                     /translation="MFSKDLEHSIGQCYKRARDARHEFMTVEHLLLALLDNPSAEAVL
                     KAIGADFPRLRGALEEAIEVSVNRLAEDDGRDTQPTLGFQRVLQRAVYHVQSSGKKEV
                     TGANVLVAIFGEKDSHAVYFLNQQDVHRLDVVNYISHGIARNGEDEPAPPQEGEARQE
                     GAEAEHKGDALAEFASNLNELAAAGRIDPLVGRAEEVERTIQVLCRRRKNNPLYVGEA
                     GVGKTALAEGLAKRIVEGDVPEVLGEAVIYSLDLGALVAGTKYRGDFEKRLKAVLAAL
                     KKLPHAILFIDEIHTIIGAGSASGGTMDASNLIKPMLASGDLRCIGSTTFQEYRGIFE
                     KDRALARRFQKIDIVEPTVGETIEILQGLKPRYEAHHGVAYADEAIKAAVDLSVKHIG
                     DRLLPDKAIDVIDEAGARQRLLPLETRKSLIDVEEIETIVAKMARIPAKQVSSSDKDV
                     LQHLERNLKMVIFGQDEAITSLTS"
     gene            complement(1498..1827)
                     /gene="clpS"
                     /locus_tag="PK818_05925"
     CDS             complement(1498..1827)
                     /gene="clpS"
                     /locus_tag="PK818_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HQY82306.1"
                     /translation="MPHKPDKPQHEHEHGVVVAPSRPQTARPPLYSVLLLNDDYTPMD
                     FVVDVLLQFFPLNLEQATQVMLHVHTRGRGVCGVFTREIAESKVAQVNEYSRMNQHPL
                     LCTMEKA"
     gene            1913..2377
                     /locus_tag="PK818_05930"
     CDS             1913..2377
                     /locus_tag="PK818_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007964277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQY82307.1"
                     /translation="MSYREGRFWQPDVTVATVVVRDGRLLCVEERSNGQLVINQPAGH
                     LEPDESLVDAAVRETREETGWNVRITHLVGAYQWKAPETRRHYLRFAFAAEAVDQIPG
                     HALDEGIVRALWLTPGELQDLAPRHRSPLVWRTVADFLGGSRHPLALVQQLA"
     gene            2374..3513
                     /gene="mnmA"
                     /locus_tag="PK818_05935"
     CDS             2374..3513
                     /gene="mnmA"
                     /locus_tag="PK818_05935"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009601444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQY82308.1"
                     /translation="MSGAAPGRERIVVGVSGGVDSSVAALLLRDAGFDVTGVFMQNWA
                     DDGSGDCRAEDDRRDAVAVCGTLGLPIRFRDFSREYRDGVFRNFLAEYAAGRTPNPDV
                     LCNREIKFKHFLDAARELGAEAIGTGHYARIDRVDGRYRLLRAKDRGKDQSYFLHQLG
                     QAQLAVTRFPLGELVKGEVRRIAAAHGLQTAGKKDSTGICFIGERDFRDFLSRYLPAK
                     PGEIRTPDGQRVGEHPGVFYFTLGQREGLNIGGVRGRAQAPWFVVGKDIGRNILYVDQ
                     GHDSPWLQSHTLRSEAAHWVAGQPPASRFRCTAQTRYRQPDEACDVEVLDDGSLAVRF
                     ERAQRAVTPGQSLVLYDGDACLGGAIILSTDAPVAGQSIPGDNAA"
     gene            3510..4118
                     /gene="hflD"
                     /locus_tag="PK818_05940"
     CDS             3510..4118
                     /gene="hflD"
                     /locus_tag="PK818_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014647076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HQY82309.1"
                     /translation="MSGIEDRVLALGGLLQALAQVRRIADTGQSETTPVRVALDSVFR
                     IDASDTEAVYGDVASLRGGLRLLRDYLANSNRDEALGKLALAVINLESRFSREAGVAG
                     QVGAGIRELAAQAARLGSAHPDVVAGLGSLYAGTVSTLRPRVMVQGNPHYLGQAGVVA
                     EIRALLLAALRSAVLWRQSGGSLWDFVFRRREMAAAIDSLMG"
     gene            complement(4174..5463)
                     /locus_tag="PK818_05945"
                     /pseudo
     CDS             complement(4174..5463)
                     /locus_tag="PK818_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
     gene            complement(5460..8609)
                     /locus_tag="PK818_05950"
     CDS             complement(5460..8609)
                     /locus_tag="PK818_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HQY82310.1"
                     /translation="MIARVIQASLAHRGWVLAITLLVAIASVLSVRQLPLDAIPDLSD
                     VQVIIRTAHPGQAPQVVEDQVTYPITTRMLAVPRAKAVRGFSMYGDSFVYVLFEDGTD
                     LYWARSRVLEYLSQVRSELPAGIDPQLGPDATGVGWVYQYALVDRSGRNDLATLRSLQ
                     DWRLRFELQRVPGVAEVASVGGFVRQYEAVVDPRRLQAYGIGFNQLLAAIRSANRETS
                     GARLEMAESEYVVRGVGYIRSLQDLGAVPTGVLKDGKAILLRDVAELRFGPAPRNGIA
                     ELDGEGEAVGGIIVMRDGANAKAVIDAVRQRLATLGSSLPPGVEIVPVYDRSGLIDRA
                     VGNLRGKLLQELLLVSLVCALFLWHARSALVAVVSLPLGVLVALAIMRWQGLNANIMS
                     LGGIAIAIGAMVDAAVVMIENAHKHLEDWASAHGGKPAEGDAHWRVIAAAALEVGPAL
                     FFSLLIAALSFLPVFMLQAQEGRMFAPLAWTKTWALAVAAGLSITLVPVLMGYWIRGR
                     FTDESGHRLTRWITAGYRPLLGFALRRPGVVVLLAVLGLASAAWPASRLGSEFMPELE
                     EGDVLYMPTTLPGLSPGQALAVLQRTDRIIKSVPEVASVFGKAGRAETATDPAPLTML
                     ETTVQFKPEAQWRPGMTRDKLLAELQQKLALPGLTGAWVPPILNRINMQVSGIRTPLG
                     VRVSGPDLASISRSAERIAQVLSQVPGTRSAFAERAADARQIDVVPDRELLARHAVTM
                     EDAQALLASAIGGEAIGRAVEGRERYPIALRTPHAWRDSPEAIAALPVVTPSGAQLPL
                     GALATVRLSEGPPMIRSEDAQLATYVFVDLDEADLGRYIARAEQALQKDARLAEGVSW
                     RWAGSYEHLQRAGERLRLAVPLTLLIVFGLLYAAFRRVAEASLVMLTLPFALVGGLWL
                     LWALGYAFSVAVAVGFIALAGVAAEFGVVMLIYLDAAVTRARAAGHLSTVDELDAALR
                     EGALRRVRPKLMTVVTIVVGLLPIAFASGAGAATMQRIAAPMIGGMLSAPLLSLVVIP
                     AVYKLWLRRRLPKANGAVP"
     gene            complement(8606..9832)
                     /locus_tag="PK818_05955"
     CDS             complement(8606..9832)
                     /locus_tag="PK818_05955"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY82311.1"
                     /translation="MSIVHFFAARRLPAWLLAAFLLAALSACTGASPGPEAATAAAPG
                     SATAPAKATTTVYSCPMHPHIQQHGPGQCPICGMDLQKREVAAGVAVSAAVVQSLGIR
                     TATPVLRDVRPRVRVPARVVADARGQARLQARVDGWIERLLVRAVGQPVAAGSVVAEI
                     YAPELVQAQEELLLGPDTAGPARERLRRLGIADADIERVRRSGSSMRRLPLRAPVAGV
                     VTELGVREGSRVTPETLVVDIAGRNAVWIEAQLFPAQRRLLGRTFAARFTLPGSPGEA
                     WSSASGSLVPVADAITQTLAVRFAVGDAADLPLGTVLDAEIDGDTRTGVLLVPASAVI
                     RTSLGDRVLVERAPRRFVPTEVTLGQRYGEEVEIRDGLAATDRIVVSGQFLLDAEASL
                     QAGLAQMQDAAAGASP"
     gene            complement(9897..>10474)
                     /locus_tag="PK818_05960"
     CDS             complement(9897..>10474)
                     /locus_tag="PK818_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82312.1"
                     /translation="EIKEEAMASSLPGDIRGNRPRSESRARMPLLRNQRTLQRHPPRS
                     NLRPEFRPPAASHRGNDQVIGRIHRHWSTARSVALAILLLVQSMMAMLPLPDAAMAMA
                     DGATTAAPHAEMPCDHGDCCDEDAPEGSSCGDSASCAAGDCSLRAVASLPPQVLMAAT
                     TLPAATILPADAPRWLLTRHDTPLLRPPIPA"
BASE COUNT         1515 a         3725 c         3664 g         1570 t
ORIGIN      
        1 gaggtcaggc tggtgatcgc ctcgtcctgg ccgaagatca ccatcttcag gttgcgctcc
       61 aggtgctgca gcacgtcctt gtcggaactg ctgacctgct tggccgggat ccgcgccatc
      121 ttggccacga tggtctcgat ttcctcgacg tcgatcaggc tcttgcgggt ctccagcggc
      181 agcaggcgct ggcgcgcgcc ggcctcgtcg atcacgtcga tcgccttgtc cggcagcagg
      241 cggtcgccga tgtgcttgac cgacaggtcc accgcggcct tgatcgcctc gtcggcgtag
      301 gcgacgccgt ggtgcgcctc gtagcgcggc ttcaggccct gcaggatctc gatggtttcg
      361 cccaccgtgg gctcgacgat gtcgatcttc tggaagcgcc gcgccagcgc gcggtccttc
      421 tcgaagatcc cgcggtattc ctggaaggtg gtggagccga tgcagcgcag gtcgccgctg
      481 gccagcatcg gcttgatcag gttggatgcg tccatggtcc cgccgctggc ggagccggcg
      541 ccgatgatgg tgtggatctc gtcgatgaac aggatcgcgt gcggcagctt cttcagcgcc
      601 gccagcaccg ccttcaggcg cttctcgaag tcgccgcggt acttggtgcc ggcgaccagt
      661 gcgccgaggt cgagcgaata gatcaccgcc tcgccgagca cctcgggaac gtcgccctcg
      721 acgatccgct tggccagccc ttccgccagc gcggtcttgc cgacgccggc ctcgcccacg
      781 tacagcgggt tgttcttgcg ccggcggcac agcacctgga tggtgcgctc cacttcctcg
      841 gcgcggccga ccagcgggtc gatcctgccg gccgccgcca gttcgttgag gttgctggcg
      901 aattcggcga gcgcgtcgcc cttgtgctcg gcctcggcgc cttcctgcct ggcctcgcct
      961 tcctgcggcg gcgcgggttc gtcctcgccg ttgcgggcga tcccgtgcga gatgtaattg
     1021 accacgtcga gccggtgcac gtcctgctgg ttgaggaagt acacggcgtg cgagtccttt
     1081 tcgccgaaga tcgccaccag cacgttggcg ccggtcactt ccttcttgcc ggaggactgc
     1141 acgtggtaga ccgcgcgctg cagcacgcgc tggaagccca gcgtgggctg ggtgtcgcgg
     1201 ccgtcgtcct cggccagccg gttgaccgag acttcgatcg cctcctccag cgcgccgcgc
     1261 aggcgcggga aatcggcgcc gatggccttg agcacggctt ccgcggacgg gttgtcgagc
     1321 agcgcgagca acaggtgttc gacggtcatg aactcgtggc gcgcgtcgcg ggcgcgcttg
     1381 tagcactggc cgatgctgtg ctcgagatcc ttgctgaaca tgggggctgg accctccgtg
     1441 ggactgctca agatgtgggg cgggcgcgca cctcaaacaa gcccccgcag cccacttcta
     1501 cgccttctcc atggtgcaca acagtggatg ctggttcatc cttgaatatt cgttcacctg
     1561 cgccaccttg gactcggcga tctcgcgggt gaacaccccg cacaccccgc gcccgcgggt
     1621 atgcacgtgc agcatgacct gggtggcctg ttccaggttg agcgggaaga actgcagcag
     1681 cacgtcgacc acgaaatcca tcggggtgta gtcgtcgttc agcagcagca ccgaatacag
     1741 cggcggacgc gcggtctgcg ggcggctggg ggcgaccacc acgccatgtt cgtgctcgtg
     1801 ctggggtttg tcgggcttgt gcggcatcgg ccaattatac gaagggcgcg cgcgtggacg
     1861 gcgtctgcgg caacagggac aatgcgggca tgacccttga aaaccaagca cgatgagcta
     1921 tcgcgaaggc cgattctggc agccggacgt gaccgtggcc accgtggtgg tccgcgatgg
     1981 ccgcctgctg tgcgtggaag agcgcagcaa cggccagttg gtgatcaacc agccggccgg
     2041 ccacctggag ccggacgaaa gcctggtgga cgccgcggtg cgcgaaaccc gcgaggaaac
     2101 cggctggaac gtgcgcatca cccatctggt cggtgcctac cagtggaagg cgcccgagac
     2161 ccgccgccac tacctgcgct tcgcgttcgc cgccgaagcg gtcgaccaga tccccggcca
     2221 cgcgctggac gagggcatcg tgcgcgcgct gtggctgacc ccgggcgagt tgcaggacct
     2281 ggcgccgcgc catcgcagcc cgctggtatg gcgcacggtg gcggatttcc tcggtggcag
     2341 ccggcacccg ctggcgctgg tgcagcagtt ggcatgagcg gcgcggcacc cgggagggaa
     2401 cgcatcgtgg tcggggtgtc gggtggcgtg gattcgtcgg tcgccgccct gctcctgcgc
     2461 gatgcgggtt tcgatgtcac cggcgtgttc atgcagaact gggccgatga tggatcgggc
     2521 gattgcaggg ccgaggacga ccgccgcgat gcggtggcgg tgtgcggcac gctgggcctg
     2581 ccgatccgct tccgcgattt ctcgcgcgaa taccgggacg gtgtgttccg gaatttcctt
     2641 gccgaatatg ccgccggccg cacgccgaac ccggacgtgc tgtgcaaccg cgagatcaag
     2701 ttcaagcatt tccttgatgc tgcccgcgag ttgggcgcgg aggccatcgg caccgggcat
     2761 tacgcgcgca tcgaccgggt ggacggccgc taccggctgt tgcgcgcgaa ggaccgcggc
     2821 aaggaccaga gctatttcct gcaccagctc gggcaggcgc agctggcggt gacgcgcttc
     2881 ccgctgggtg aactggtcaa gggcgaggtg cgccggatcg cggccgcgca tggcctgcag
     2941 accgccggca agaaggattc caccggcatc tgcttcatcg gcgagcgcga cttccgcgac
     3001 ttcctgtcgc gctacctgcc ggcgaagccg ggcgagatcc gtacgccgga cgggcaacgc
     3061 gtgggcgaac acccgggtgt gttctatttc accctgggcc agcgcgaggg cctgaacatc
     3121 ggcggcgtgc gcgggcgcgc gcaggcaccg tggttcgtgg tcggcaagga catcgggcgc
     3181 aacatcctgt atgtcgacca gggccacgac agcccgtggc tgcaatcgca cacgctgcgc
     3241 agcgaagctg cgcactgggt cgccgggcag ccgcccgcgt cgcggttccg ctgcacagcg
     3301 cagacccgct atcggcagcc cgacgaggcc tgcgatgtcg aggtgctgga cgatggcagc
     3361 ctggcggtgc gcttcgagcg cgcgcagcgc gcggtcaccc cgggccagtc gctggtgctg
     3421 tacgacggcg atgcctgcct cggtggtgcc atcatcctgt cgaccgacgc ccccgtcgcc
     3481 ggccaatcca ttcctgggga caacgctgca tgagtggcat cgaagaccgt gtactcgcgc
     3541 tgggcgggtt gttgcaggcg ctggcgcagg tgcgccggat cgccgacacc gggcagtcgg
     3601 agaccacgcc ggtgcgggtg gcgctggaca gcgtgttccg gatcgacgcg tcagataccg
     3661 aagcggttta tggcgacgtt gcatcgctgc gcggcggcct gcggctgctg cgcgactacc
     3721 ttgccaacag caaccgcgac gaggcgctgg gcaagctggc gttggcggtg atcaacctgg
     3781 agagccgctt cagccgcgaa gccggggtcg ccggccaggt gggcgccggc atccgcgagc
     3841 tggcggcgca ggccgctcgc ctcggcagcg cgcacccgga cgtggtcgcc gggcttggca
     3901 gcctgtatgc agggaccgtc agcaccctgc gtccgcgggt gatggtgcaa ggcaacccgc
     3961 attaccttgg ccaggccggg gtggtggcgg agatccgtgc cctgctcctg gccgcgctgc
     4021 gttcggcggt gctgtggcgg caaagcggcg gcagcctgtg ggatttcgtg ttccggcggc
     4081 gcgagatggc ggctgcgatc gattcgctga tggggtaagg cggggcgtgg tcgcatgccg
     4141 gccatccgtg gccatctcac gcccggcagc acgctccagc gccagcacct gcagccacag
     4201 ggtgcgggat gcggaaaggt gttcccgttc tgccagcacg cgctcgcggg ttgccgtgcg
     4261 ctgcgccgtg cgatcgacga tcccgcgccg gtagctgcgc tcgaccagca gctgcagttg
     4321 ccgctgctgc tgcaggtgcc ggtcggcctg cgcgacggcg tcctgcgcga tcagcaccgt
     4381 cgccagcgcg cgctccagct ccagcgggat caggcggcga acggcttcgg cctggtgggt
     4441 ggcctcggcg acccgcgcat cggcgagtgc ggtccgcgcc tggccactgt cgaacaccgg
     4501 cagggtgacc tgcaattcgg gacccagcat cgccatgcca ttggcctcgc gcatgcccgc
     4561 cagtccggct gatggcaggg tggcgcgcac cgccccgctc tccaggcggt gcgcggcctg
     4621 cgcctgctcc aatcgggcgc tcgccagctg cgcgtcgggg cgatggttcg ccgcccacgc
     4681 ctgccattcg cgatcgtcat ggttcgccag cgccggtacc ggaagcgcgg ccggcagcgt
     4741 gatcgcattc gcgctggcca ggcccagggc ggccgccagt gcgctgcgcg cctgcaccag
     4801 tgccgattcg gcaacgcccg tttcgtgcgc ctggcgcgcg tgcgcggact gctgcgccag
     4861 ctggccactg gccgccgcga tgccgcgctg cacttcgcgc cggctgagca cctgcgcttc
     4921 ctcgtccagc gccagcagcg cgcgttccag cgccagcgcc tgctgcgcga accatgcctg
     4981 caccagcgcc tggcgggtgt cctgcgccag cgccaccagt tgcagcagga cgtccgcctg
     5041 gcgttggcgt gcgtcggcat cggccagcgc gatccggcgg ctgcgggtca gcacgtcgta
     5101 caggctttgc atcaacccgt attcgagctg gaagcggccg ccgccctcgg gccgcagcgc
     5161 catcatcgac agcatcgggt tgcccaccag gccggcctgc acgcgctccg cgtacgcggc
     5221 atccagccgt gccagcgccg cgcgcacctg cgggttgttg gcgaaggcga cctgcaccgc
     5281 cctttccggc gacagtggca gcgacaggtc gatgctgtcc gctgcggacg gcaaccgcgc
     5341 cgcggggctg accgccgcca gccccgactg caaggacgtg gaggattgtc gcggtggcgc
     5401 actcgcgcag ccggcaacca gcaatgccag cgcgatcgcg caggccaggg tcgaggtgtt
     5461 catggcaccg ctccgttcgc cttcggcagg cgccgccgca gccacaactt gtagaccgcg
     5521 ggaatcacga ccagcgacag cagcggcgcg ctgagcatgc cgccgatcat cggcgcggcg
     5581 atgcgctgca tggtggcggc acctgccccg ctggcgaagg cgatcggcaa cagcccgacg
     5641 acgatcgtga ccaccgtcat cagcttcggc cgcacccgtc gcagtgcgcc ttcgcgcaag
     5701 gccgcgtcga gctcgtcgac cgtcgacagg tgccccgccg cgcgtgcgcg ggtcaccgcg
     5761 gcatcgaggt agatcagcat caccaccccg aactcggcgg cgaccccggc cagggcgatg
     5821 aagccgaccg cgaccgccac cgagaacgcg tagcccagcg cccacagcag ccacagcccg
     5881 ccgaccagcg cgaacggcag ggtcagcatg accaggctgg cctccgccac ccgccggaag
     5941 gcggcataga gcagcccgaa cacgatcagc agggtcaacg gcaccgccag ccgcaggcgt
     6001 tcgccggcgc gctgcaggtg ctcgtaactg ccggcccagc gccagctgac gccctccgcc
     6061 agccgagcgt ccttctgcag cgcctgctcg gcgcgggcga tgtagcggcc caggtcggct
     6121 tcatcgaggt cgacgaacac gtaggtggcc aactgcgcgt cctcgctgcg gatcatcggc
     6181 gggccctcgg acaggcggac cgtggccagc gcgcccagcg gcagttgcgc gccggagggt
     6241 gtcaccaccg gcaacgcggc gatcgcttcg ggcgagtcgc gccaggcatg cggggtgcgc
     6301 agcgcgatcg ggtaacgctc gcggccttcc accgcgcgcc cgatcgcctc gccaccgatc
     6361 gccgaggcca gcaacgcctg cgcgtcctcc atcgtcactg cgtgccgcgc gagcagttcc
     6421 cggtccggta cgacgtcgat ctggcgcgca tcggcggcgc gttcggcgaa ggccgagcgg
     6481 gtgcccggca cctgcgacag cacctgggcg atgcgttcgg cgctgcggct gatgctggca
     6541 aggtccggac cgctgacgcg cacccccaat ggcgtgcgga tgccgctgac ctgcatgttg
     6601 atgcgattga ggatcggcgg gacccaggca ccggtcaggc cgggcagcgc gagtttctgc
     6661 tgcaactccg ccagcagttt gtcgcgggtc atcccgggcc gccactgcgc ttccggcttg
     6721 aactgcacgg tggtctcgag catggtcagc ggcgccggat cggtagcggt ttcggcgcgc
     6781 ccggccttgc cgaacacgct cgccacttcg ggcaccgact tgatgatgcg gtcggtgcgc
     6841 tgcagcaccg ccagcgcctg gcccggcgag agcccgggca gcgtggtcgg catgtacagc
     6901 acgtcgccct cttccagttc gggcatgaac tcgctgccca gccggcttgc cggccaggcc
     6961 gcgctggcca gccccagcac cgccagcaac acgacgactc ccggccggcg cagggcgaac
     7021 cccagcagcg gccggtagcc ggcggtgatc cagcgggtca gccggtgccc ggactcgtcg
     7081 gtgaagcggc cgcggatcca gtaccccatc agcactggca ccagggtgat cgacaggccc
     7141 gcggccaccg ccagcgccca ggtcttggtc catgccagcg gcgcgaacat ccgcccttcc
     7201 tgcgcctgca gcatgaacac cggcaggaac gacagcgcgg cgatcagcag cgagaagaac
     7261 agcgccggcc cgacctcgag cgcggcggcg gcgatcaccc gccaatgcgc gtcgccctcg
     7321 gcaggcttgc cgccatgggc gctggcccag tcctcgagat gcttgtgcgc gttctcgatc
     7381 atcaccaccg cggcatccac catcgcgccg atcgcgatcg cgatgccgcc cagcgacatg
     7441 atgttggcgt tcaacccctg ccagcgcatg atcgccagcg ccaccagcac ccccagcggc
     7501 aggctgacca ccgcgaccag cgccgagcgc gcatgccaca ggaacagcgc gcacaccagc
     7561 gacaccagca gcagttcctg cagcagcttg ccgcgcaggt tgccgaccgc gcgatcgatc
     7621 agcccggagc ggtcgtacac cggcacgatc tccacccccg gcggcagcga ggacccgagc
     7681 gtggccagcc gctgcctgac cgcgtcgatc accgccttcg cgttcgcgcc atcgcgcatc
     7741 acgatgatgc cgccgaccgc ctcgccctcg ccgtcgagct cggcgatgcc gttgcgcggt
     7801 gccggcccga acctgagttc ggcgacatcg cgcagcagga tcgccttgcc gtccttcagc
     7861 accccggtgg gtaccgcgcc gaggtcctgc agcgaccgga tgtagccaac cccgcgcacc
     7921 acgtattcgc tctcggccat ctccaggcgc gcgccgctgg tctcgcggtt ggcgctgcgg
     7981 atcgccgcga gcagctggtt gaaaccgatg ccgtacgcct gcagccggcg cggatcgacc
     8041 accgcctcgt actggcggac gaagccgccg acgcttgcca cttccgcgac ccccggcacc
     8101 cgctgcagtt cgaagcgcag gcgccagtcc tgcagcgaac gcagcgtggc aaggtcgttg
     8161 cgcccgctgc gatcgaccag cgcgtactgg tagacccagc cgacgccggt cgcatccggg
     8221 cccagttgcg ggtcgatccc ggcgggcaat tcggagcgca cctgcgagag gtattccagc
     8281 acccgcgaac gcgcccagta caggtcggta ccgtcctcga acagcacgta cacgaaggag
     8341 tcgccataca tcgagaaacc gcgcaccgcc ttcgcgcgcg gcaccgcgag catgcgggtg
     8401 gtgatcggat aggtgacctg gtcctccacc acctgcggcg cctgccccgg atgcgcggtg
     8461 cggatgatca cctgcacgtc ggacaggtcg gggatggcat ccagcggcag ctgccgcacc
     8521 gacagcacgc tggcgatggc caccagcagc gtgatcgcca gcacccagcc gcgatgcgcc
     8581 agcgatgcct ggatcacccg cgcgatcatg gcgaagcccc ggccgcggcg tcctgcatct
     8641 gcgcaagccc cgcctgcagg ctggcctcgg cgtcgagcag gaactggccg gaaaccacga
     8701 tgcgatcggt cgctgccagc ccgtcgcgga tctccacctc ttcgccatag cgctggccga
     8761 gggtgacttc ggtcggcacg aagcggcgtg gcgcgcgttc caccagcacg cgatcgccca
     8821 gcgaggtccg gatcaccgcc gaggcgggca ccagcagcac gccggtgcgc gtatcgccgt
     8881 cgatctcggc atccagcacg gtccccagcg ggaggtcggc ggcatcgccc accgcgaacc
     8941 gcaccgccag ggtctgggtg atcgcatcgg ccaccggcac caggctgccg ctggcgctgc
     9001 tccacgcctc cccgggcgac cccggcaggg tgaagcgcgc cgcgaacgtg cgccccagca
     9061 ggcgccgctg cgccgggaac agctgcgcct cgatccacac cgcgttgcgc ccggcgatat
     9121 ccaccaccag cgtttccggg gtcacccgcg agccttcgcg aacgcccagt tcggtgacca
     9181 ccccggccac cggcgcgcgc agcggcaggc gccgcatcga gctgccggag cggcgcaccc
     9241 gctcgatgtc ggcgtcggcg atgccaagcc ggcgcaggcg ttcgcgcgcg ggcccggcgg
     9301 tgtccgggcc gagcagcagt tcctcctgcg cctgtaccag ttccggggcg tagatctcgg
     9361 cgaccacgct gccggcggcc accggttgcc cgaccgcgcg caccagcagg cgttcgatcc
     9421 agccgtccac ccgcgcctgc aggcgtgcct gcccgcgcgc atcggcgacc acccgcgccg
     9481 gcacccgcac gcgcggccgc acgtcgcgca gcaccggggt ggcggtgcgg atgcccaggc
     9541 tctggacgac cgcggcactg accgcgaccc cggcggcgac ctcgcgcttc tgcaggtcca
     9601 tgccgcagat cgggcactgg cccggcccgt gctgctggat gtgcgggtgc atcgggcagc
     9661 tgtagacggt ggtggtggcc ttcgctggcg cagtggcgct ccccggcgcg gctgcggtgg
     9721 cggcctcggg cccggggctg gcgccggtgc aggcggacag cgcagcaagc aggaatgcgg
     9781 caagcagcca cgccggcagg cgccgcgcgg cgaaaaaatg aacgatcgac atggtggttc
     9841 ccggtgaacg aagacacgcg cgcacgccgt ggcggcgcgg tttcaggcgt ctggcgtcag
     9901 gccgggatcg gtggtcgcaa caacggggtg tcgtggcgtg tcagcagcca gcgcggggcg
     9961 tcggcgggca ggatcgtggc ggctggcagc gtggtggcgg ccatcagcac ctgcgggggc
    10021 aggctggcca cggcgcgcag gctgcagtcg cctgcggcgc aggacgcgct gtcgccacag
    10081 ctgctgcctt cgggcgcatc ctcgtcgcag caatcgccgt ggtcgcaggg catttcggcg
    10141 tgcggggcgg cggtggtcgc gccgtccgcc atcgccatcg ccgcgtcagg cagcggcagc
    10201 attgccatca tcgattgcac cagcagcagg atcgccaggg cgacgctgcg tgcggtggac
    10261 caatggcggt ggatgcggcc gatcacttga tcgttgcctc gatggcttgc ggcaggtggg
    10321 cggaactccg ggcgcagatt agacctcggg gggtgccgct gcaatgttcg ctgattccga
    10381 aggagcggca tcctcgcccg gctctccgat cgcggcctgt ttccgcgaat gtcccccggc
    10441 aatgatgaag ccattgcctc ctcctttatt tcgc
//
LOCUS       DAORUK010000066        10332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_6820_length_10332_cov_17.187701, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000066 DAORUK010000000
VERSION     DAORUK010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10332
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6820_length_10332_cov_17.187701"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PK818_05965"
     CDS             <1..651
                     /locus_tag="PK818_05965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HQY82313.1"
                     /translation="VHAARRHGCRVTTTTISREQHALASQRVADAGLQDRVTVLLSDY
                     RDLQGQYDKLVSIEMIEAIGAQYLDTYFATLCRLLKPEGLALVQAITIEDHRYEQALR
                     SVDFIKRHIFPGSFIPSIAAMLAAKARSTDLALVHLEDFGPSYARTLLHWRERFLARR
                     DEARAQGYDERFLRMWEFYLAYCEGGFRERSIGVAHLLMAKPGWRGGSYLPGLLEA"
     gene            653..1189
                     /locus_tag="PK818_05970"
     CDS             653..1189
                     /locus_tag="PK818_05970"
                     /inference="COORDINATES: protein motif:HMM:NF022537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HQY82314.1"
                     /translation="MRFWINLLGYQAAWFVAVGFAARGLAWPGIVACLGFAALTWWQS
                     PRRRSDLRLVAAALACGLLLDGALAATGWLAYAAPLPALPAPAWIVALWAAFAMTLQH
                     SMQWLLARPLAAVLFGAMGGPLAYSGAARGFDAVVFNMPLQATLLLGFGWGFAMWLLV
                     AIARVDPATTLVQREVSA"
     gene            1186..1968
                     /locus_tag="PK818_05975"
     CDS             1186..1968
                     /locus_tag="PK818_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HQY82315.1"
                     /translation="MNPWQQLLLVWLVAALAQTLGWRWQRTRANAGIVDVVWSFGVGG
                     AAVLAAATGGGALLPRALLAVLGGLWGLRLGLYLWHRVRGEAEDGRYAQLRLRWGNDQ
                     RKWFAMFQFQALLIALFSLPFLAVAANPSAAWNAWFVAGIAMWLASVGGEAIADRQLA
                     DFRANPASKGKTCRAGLWRYSRHPNYFFEWLHWFAYVLLAIGSPIAWLAWAGPVVMYV
                     FLRWISGIPYTEAQALRTRGEDYARYQRSTPMLIPWFPKEDR"
     gene            1986..3047
                     /locus_tag="PK818_05980"
     CDS             1986..3047
                     /locus_tag="PK818_05980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HQY82316.1"
                     /translation="MELPDDRSAPGLLGLAERGLVPDALLRAGIRRMCAQRLRDEHAG
                     DPAAAARRTTALIAELRQSPVAIHTDAANRQHYELPPAFFHACLGPRLKYSSCFYPTG
                     NETLAQAEEAMLALYGERAQLADGQDILELGCGWGSLTLWMAEHYPNARITAVSNSRP
                     QREFIEARCRERGFANVRVLTCDVNRLELPAEGFDRCVSVEMFEHMRNYATLLGRIGG
                     WLRADGKLFVHIFCHRTLLYPFETAGEDNWMGRHFFTGGLMPAADTLLWFQDALRIDD
                     RWLLDGTHYQRTANHWLREQDSRRDEVMAILRKAYGDAAPLWYQRWRMFWMACAELFG
                     YDHGREWMVAHYRFSKESA"
     gene            3044..3595
                     /locus_tag="PK818_05985"
     CDS             3044..3595
                     /locus_tag="PK818_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HQY82317.1"
                     /translation="MTRTLLAALGLGLALSACASTPPTIPPVANVDLQRFMGDWYVIA
                     HIPSMPEREAFNAIESYQLDDEGRIRTTFQFRKGSFDAKLKTMEPVGRVVPGTNNAVW
                     DMQFVWPIQAEYVIVDLSDDYSQTVIGRSKRDYLWIMARTPSIADADYAALVAKAKAL
                     GYDTTQLRKVPQQWPETAADARR"
     gene            complement(3600..4886)
                     /gene="aroA"
                     /locus_tag="PK818_05990"
     CDS             complement(3600..4886)
                     /gene="aroA"
                     /locus_tag="PK818_05990"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011259050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HQY82318.1"
                     /translation="MATPAGPLHGTVAVPGDKSVSHRAIMLGAIAEGVTRIEGFLEGE
                     DTRATAAIFRRLGVQIDAPSDGVRIVHGVGLHGLQPSSEPLDCGNAGTAMRLLAGLLA
                     GQRFDSVLVGDASLSKRPMRRVIEPLAAMGALIESDGGCAPLRIRGGRALQGIDASLP
                     VASAQVKSALLLAGLYANGETHVREPHPTRDYSERMLAAFGVPCAFVPGEAALLGGFP
                     LQARDVSVPADFSSAAFFIVAATLVPGSELLLRRVGMNPRRTGLLHVLRAMGADIAEE
                     NAGEQGGEPVADLRVRHAPLRGIEVPVEHVADMIDEFPALFVAAACAEGATLVRGAAE
                     LRVKESDRIATMATGLRALGIVVDETPDGATIHGGGFHGGIVESHGDHRIAMAFAVAA
                     QLATGEVRIGDVANVATSFPGFDTLARAAGFGLRAA"
     gene            complement(4916..6046)
                     /gene="pheA"
                     /locus_tag="PK818_05995"
     CDS             complement(4916..6046)
                     /gene="pheA"
                     /locus_tag="PK818_05995"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HQY82319.1"
                     /translation="MAAKKQEAKPGKAAKVGTQPDLADVRAQIDGLDRRIQELIAERA
                     QWAHQVGKAKGKLAAAVDYYRPEREAQVLRRVVDRNDGPLSDEELVRLFREIMSACLA
                     QQEPLKIGYLGPEGTFSQQAVYKHFGHSAHGLPMASIEEVFQEVSAGSADFGVVPVEN
                     SGQGTIQVTLDMFLTSPLRICGEVELRVHQFLLSRSGRIEDVERIFAHPQAFAQCSGW
                     LRQHLPRVEKIPTSSNAEGARRARSADDSAAIAGESAGQVYALKKIAGPIEDRSDNTT
                     RFLVIGRSAFPPSGHDRTSLLVYIRDRPGALYGVLEPLARRGISMSRIESRPEHGHKW
                     QYAFFIDVAGHCEESPLKDALAEVGEKGDEVRVLGSYPVAIL"
     gene            complement(6145..7230)
                     /gene="serC"
                     /locus_tag="PK818_06000"
     CDS             complement(6145..7230)
                     /gene="serC"
                     /locus_tag="PK818_06000"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HQY82320.1"
                     /translation="MSRAFNFSAGPATLPEPVLRQAQAEMLEFGNAGASIVELSHRGP
                     EFIAVAQRTEADLRTLLSIPDDYAVLFTSGGATTTQALLPLNFAAAGQPVDYVVTGHW
                     GKTALKQAKPYAAANVAASSEADGFRSIPARASWNLSPDAAYVHITANETIHGVEFRE
                     LPDTGSVPLIADFSSSIASEPLDIRRFGAIYAGAQKNLGPVGIGVLIVDRELLERAGQ
                     PRADIFDFRSQLKAESMLNTPPSWNWYMLGLTVKWMLDEGGVAEFAKRNARKAAVLYD
                     VIDRSGGFYRNEVEAAVRSRMNVPFFLFDEALDKPFLKEAADAGLISLKGHRALGGMR
                     ASIYNAMPEQGVQALADFMRDFEARNG"
     gene            7407..8375
                     /gene="msrP"
                     /locus_tag="PK818_06005"
     CDS             7407..8375
                     /gene="msrP"
                     /locus_tag="PK818_06005"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HQY82321.1"
                     /translation="MPQRHPALPADADAITEEAVYRDRRRLLAAFGLLPTLGLAGCSE
                     AAPPASKAVVTPAQAKSGFRTAEEQTRELDATTYNNFYEFGTNKDDPANAPKTLRTKP
                     WSVAVGGHCARPGTLALEDLLKGLPSQERIYRMRCVEGWSMVIPWLGVPLADVLKRFE
                     PTSKAKYVAFTSLADPRQMPGVRVPTLDWPYREGLRIDEAMHPLTLLATGMYGKALPQ
                     QNGAPLRLVVPWKYGFKGIKSIVAITFVERMPKTSWNMSQPSEYGFYSNVNPAVDHPR
                     WSQKSERRIAGSASKLFAERIPTRPFNGYAEQVASLYAGMDLKKWF"
     gene            8578..9198
                     /gene="msrQ"
                     /locus_tag="PK818_06010"
     CDS             8578..9198
                     /gene="msrQ"
                     /locus_tag="PK818_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HQY82322.1"
                     /translation="MRSLILPKSIVHLAALTPLAILLWQAWSVAKTGGNALGADPVAE
                     IEHRLGLWALRFLAVTLAITPLRQLTGWNVLVRFRRMLGLYAFAYATLHFAAYLGLDL
                     RGYWTQVFEDIAKRPYITVGFTAWLLLVPLAVTSTSGWIRRLGRNWARLHMLVYPVAV
                     LAVLHFWWLVKSDLREPALYAVIVALLLGWRTWKRWKPRTSLSARA"
     gene            complement(9170..9442)
                     /locus_tag="PK818_06015"
     CDS             complement(9170..9442)
                     /locus_tag="PK818_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HQY82323.1"
                     /translation="MSKIDIRHPHSLPKAKARKAIEEVAKKLAEKFDMDYGWEGDTLN
                     FSRSGVDGHIMLEPNDLHVHAKLGFLTAMFKEPIEGEIKRVLKEKF"
     gene            9538..10326
                     /locus_tag="PK818_06020"
     CDS             9538..10326
                     /locus_tag="PK818_06020"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HQY82324.1"
                     /translation="MNELRLRFGDGDQPDLVLGSGVHALGRLPTGLGPVDAEQPWLLQ
                     LCNDRRGIWLTVADDLRGVHVNGRPVQHVAMLRAGDSIHVDGNELLLTAANDGRALPP
                     PAAQRDPIGNLRLVLRGVGGAHHGRSISLETPRRIGRAADADIRIEGPGIAERHALLD
                     AVNGQAVLREAGADVLVNGQRVRQAVLRAGDQIAFDVQHRFVLEGPPPATAAPAAGRP
                     RAAADPDAAPEPPRRSWLQRMPWLLVAAILLAAALSALLLFGAR"
BASE COUNT         1493 a         3621 c         3600 g         1618 t
ORIGIN      
        1 gtgcatgccg cgcgccgcca cggctgccgg gtcaccacca ccacgatctc ccgcgaacag
       61 cacgcgctgg ccagccaacg ggtggccgat gccggcctgc aggaccgggt cacggtgctg
      121 ctgtcggact accgcgacct gcagggccag tacgacaagc tggtctcgat cgagatgatc
      181 gaggcgatcg gcgcgcaata cctggacacc tatttcgcca cgctctgccg cctgctgaag
      241 ccggaagggc tggcgctggt gcaggcgatc accatcgagg accaccgcta cgagcaggcg
      301 ctgcgcagcg tggacttcat caagcggcac atcttccccg gctcgttcat cccgtcgatc
      361 gcggcgatgc tggcggcgaa ggcccgcagc accgacctcg cgctggtgca cctggaggat
      421 ttcgggccgt cgtacgcgcg cacgctgctg cattggcgcg aacgcttcct ggcacgccgc
      481 gatgaagccc gcgcgcaggg ctacgacgag cgcttcctgc gcatgtggga gttctacctg
      541 gcctattgcg aaggcggctt ccgcgaacgc tccatcggcg tcgcccacct gctgatggcg
      601 aagccgggct ggcgcggcgg ctcctacctg cccggcctgc tggaggcctg agatgcgctt
      661 ctggatcaac ttgctgggct accaggcggc gtggttcgtc gcggtcggct tcgccgcgcg
      721 cgggctggcg tggccgggca tcgtcgcctg cctgggcttc gcggcactga catggtggca
      781 atcgccacgg cgccggtccg acctgcggct ggtggcggcg gcgctggcct gcgggctgct
      841 gctggacggt gcgctggcgg ccaccggctg gctggcctat gccgcaccgc tgcctgcatt
      901 gccggcgccg gcatggatcg tggcgctgtg ggcggccttc gcgatgaccc tgcagcattc
      961 catgcagtgg ctgctggcgc gcccgctggc cgcggtcctg ttcggtgcga tgggcggccc
     1021 cctggcgtac tcgggcgccg cgcgcggctt cgacgcggtg gtgttcaaca tgcccctgca
     1081 ggcgacactg ctgctgggct tcggctgggg cttcgcgatg tggctgctgg tcgccatcgc
     1141 ccgcgtcgac ccggcaacca ctctggtcca acgcgaggtc tccgcatgaa tccgtggcag
     1201 caactcctgc tggtgtggct ggtcgccgcg ctggcgcaaa ccctcggttg gcgctggcag
     1261 cgcacgcgcg cgaacgcggg catcgtggac gtggtctggt cgttcggcgt gggcggggcg
     1321 gcggtgctgg cggccgccac cggtggcggc gcgctgctgc cacgcgcgct gctggcggtg
     1381 ctcggcggcc tgtggggcct gcgcctgggc ctgtacctgt ggcaccgcgt gcgcggcgag
     1441 gcagaggacg gccgctatgc ccagctgcgg ctgcgctggg gcaacgacca gcgcaagtgg
     1501 ttcgcgatgt tccagttcca ggcgctgctg atcgcgttgt tctcgctgcc gttcctggcg
     1561 gtcgcggcca acccgtcggc ggcgtggaat gcgtggttcg tggccggcat cgcgatgtgg
     1621 ctggccagcg tcggcggcga ggccatcgcc gaccgccagc tcgcggattt ccgcgccaat
     1681 ccggccagca agggcaagac ctgccgcgcc gggctgtggc gttactcgcg gcatccgaac
     1741 tacttcttcg agtggctgca ctggttcgcc tacgtgctgc tggccatcgg ttcgccgatc
     1801 gcgtggctgg cctgggccgg gccggtcgtc atgtacgtgt tcctgcgctg gatcagcggc
     1861 attccctaca ccgaggcgca ggccctgcgc acgcgtggcg aggactacgc gcgctaccag
     1921 cgcagcaccc cgatgctgat cccgtggttt ccgaaggagg accgatgaac gcacctgccg
     1981 acgcgatgga attgcccgac gaccgatccg cccccggcct gctgggcctg gccgaacgtg
     2041 ggctggtgcc ggacgcgctg ctgcgtgccg ggatccgccg gatgtgcgcg cagcgcctgc
     2101 gcgacgaaca cgcgggcgac ccggccgccg ccgcgcgccg caccaccgcg ctgatcgccg
     2161 aactgcggca gagcccggtc gcgatccaca ccgacgccgc caaccgccag cattacgagc
     2221 tgccgccggc gttcttccac gcctgcctcg gaccgcgcct gaagtattcg tcctgcttct
     2281 atcccaccgg caacgagacc ctggcccagg ccgaggaggc gatgctggcg ctgtacggcg
     2341 aacgcgcgca gctggcggac gggcaggaca tcctggaact gggctgcggc tggggctcgc
     2401 tgacgctgtg gatggcggag cattacccga atgcccgcat caccgcggtc tccaactcgc
     2461 gcccgcagcg cgaattcatc gaggcgcgct gccgcgaacg cggcttcgcc aacgtgcgcg
     2521 tgctgacctg cgacgtgaac cggctggagc tgccggccga aggcttcgac cgctgcgtct
     2581 cggtcgagat gttcgagcac atgcgcaact acgccaccct gctcggccgc atcggcggct
     2641 ggctgcgcgc ggatggcaag ctgttcgtgc acatcttctg ccaccgcacc ctgctgtatc
     2701 cgttcgagac cgcgggcgag gacaactgga tgggccggca tttcttcacc ggtggcctga
     2761 tgccggcggc ggacaccctg ctgtggttcc aggacgcgct gcggatcgac gaccgctggc
     2821 tgctggacgg cacccactac cagcgcaccg ccaaccactg gctgcgcgag caggattccc
     2881 gccgcgatga agtgatggcg atcctgcgca aggcctacgg cgacgccgcg ccgctctggt
     2941 accagcgctg gcgcatgttc tggatggcct gcgccgaact gttcggctac gaccacggcc
     3001 gcgaatggat ggtcgcccac taccgcttca gcaaggaatc cgcatgaccc gcaccctgct
     3061 cgccgcgctc ggcctcggcc tggccctgtc cgcctgcgca tcgaccccgc ccaccatccc
     3121 gccggtggcg aacgtggacc tgcagcgctt catgggcgac tggtacgtga tcgcccatat
     3181 cccctcgatg ccggagcgcg aagccttcaa cgccatcgag tcctaccagc tggatgacga
     3241 aggccgcatc cgcaccacct tccagttccg caagggcagc ttcgacgcga agctgaagac
     3301 catggagccg gtcgggcggg tggtccccgg caccaacaac gcggtctggg acatgcagtt
     3361 cgtatggccg atccaggccg aatacgtgat cgtcgacctg tcggacgact acagccagac
     3421 cgtgatcggc cgcagcaagc gcgactacct gtggatcatg gcgcgcaccc ccagcatcgc
     3481 cgacgccgac tacgcggcgc tggtggcgaa ggcgaaggcg ctcggctacg acaccaccca
     3541 gctgcgcaag gtgccgcagc agtggccgga aaccgcggcc gacgcgcgcc gctgatgcct
     3601 taggccgcgc gcagcccgaa gccggcggcg cgcgcaaggg tgtcgaagcc ggggaatgag
     3661 gtggccacgt tggccacatc gccgatccgc acctcgcccg tagccaactg cgcggccacc
     3721 gcgaatgcca tcgcgatgcg gtggtcgcca tgcgattcga cgatgccgcc gtgaaatccg
     3781 ccgccatgga tggtcgcgcc atccggggtt tcgtcgacca cgatgcccaa cgcgcgcagg
     3841 cccgtggcca tggtcgcgat gcggtcggat tccttgaccc gcagctcggc cgcgccacgc
     3901 accagggtgg ccccttcggc gcaggcggcg gccacgaaca gtgccgggaa ctcgtcgatc
     3961 atgtccgcca cgtgttccac cggcacctcg atgccgcgta gcggcgcatg ccgcacgcgc
     4021 aggtcggcca ccggttcgcc gccctgctcg ccggcgttct cctcggcgat gtccgcaccc
     4081 atcgcacgca gcacgtgcag caggccggtg cggcgcggat tcatgccgac ccggcgcaac
     4141 agcagctccg agcccggcac cagggtggcc gcgacgatga agaacgccgc ggacgaaaaa
     4201 tccgccggca cgctgacgtc gcgcgcctgc agcgggaagc cgcccagcag ggccgcttcg
     4261 cccggcacga acgcacaggg cacgccgaac gcggcgagca tgcgttcgct gtagtcgcgg
     4321 gtgggatgcg gctcgcgcac gtgggtctcg ccattcgcgt acaggccggc cagcagcagc
     4381 gcggatttca cctgcgcgct tgcgaccggc aagctggcgt cgatgccctg caacgcccgt
     4441 ccgccgcgga tgcgcagcgg cgcgcagccg ccatcggatt cgatcagcgc gcccatcgcc
     4501 gccagcggct cgatcacccg ccgcatcggc cgcttcgaca gggacgcgtc gccgaccagc
     4561 acgctgtcga agcgctgccc ggccagcaac ccggccagca gccgcatcgc ggtgccggca
     4621 ttgccgcagt ccagcggttc ggacgatggc tgcaagccgt gcaggcccac gccatgcacg
     4681 atgcgcacgc catcggatgg ggcgtcgatc tgtacgccga gccggcggaa gatcgcggcg
     4741 gtggcgcggg tgtcctcgcc ctcaaggaag ccctcgatgc gggtcacgcc ctcggcgatc
     4801 gcgccaagca tgatcgcgcg atgcgacacc gacttgtcgc cgggcaccgc cacggttccg
     4861 tgcagcgggc cggcgggcgt ggcgatccag gcgctggcgg caggcgcgcc tgccgtcaca
     4921 ggatcgccac cgggtaggag ccgagcaccc gcacctcgtc gcccttctcg ccgacttcgg
     4981 ccagcgcgtc cttcagcggc gattcctcgc aatggccggc cacgtcgatg aagaacgcgt
     5041 attgccactt gtggccgtgc tcggggcgcg actcgatccg gctcatgctg atcccgcgcc
     5101 gcgccaacgg ctccagcacg ccgtacagcg cgccggggcg gtcgcggatg tacaccagca
     5161 gcgaggtgcg gtcgtggccg gacggcggga acgcactgcg gccgatgacc aggaagcggg
     5221 tggtgttgtc gctgcggtcc tcgatcgggc cggcgatctt cttcagcgcg tagacctgcc
     5281 cggccgactc gccggcgatc gccgccgaat catccgcgct gcgcgcgcgg cgcgcgcctt
     5341 cggcattgct cgaggtcggg atcttctcga cccgcggcag gtgctggcgc agccagcccg
     5401 agcactgcgc gaacgcctgc ggatgcgcga agatccgttc gacgtcctcg atccggccgc
     5461 tgcgcgacaa caggaactgg tgcacccgca gctcgacttc gccacagatc cgcagcggcg
     5521 aggtcaggaa catgtccagg gtcacctgga tggtgccctg cccggagttc tccaccggca
     5581 ccacgccgaa gtcggcgctg cccgcggaaa cttcctggaa cacctcctcg atgctggcca
     5641 tcggcaggcc gtgggcggaa tggccgaaat gcttgtacac cgcctgctgg ctgaaggtgc
     5701 cttccgggcc gaggtagccg atcttcagcg gctcctgctg ggccaggcag gccgacatga
     5761 tctcgcggaa caggcgcacc agttcctcgt cggacagcgg accgtcattg cggtccacca
     5821 cccgccgcag cacctgtgct tcgcgctccg ggcggtagta gtccaccgcc gcggcaagct
     5881 tgcccttggc cttgccgacc tggtgcgccc attgcgcgcg ctcggcgatc agttcctgga
     5941 tgcggcggtc caggccgtcg atctgcgcgc gcacgtcggc caggtcgggc tgggtgccga
     6001 ccttcgcggc cttgccgggc ttcgcttcct gcttcttcgc ggccatcgct tcgttcctgg
     6061 catgtgtttg caggagccca ccccgtccgc gaccgcctgt tcgccaggct acggccaccg
     6121 acagggtccg ctcctacagg gtcatcaacc gttcctggcc tcgaaatcgc gcatgaaatc
     6181 ggccagcgcc tgcacgccct gctccggcat cgcgttgtag atcgaggcgc gcatgccgcc
     6241 gagggcgcgg tggcccttca gcgagatcag cccggcatcc gcggcttcct tgaggaaggg
     6301 cttgtccagg gcctcgtcga acaggaagaa cggcacgttc atgcgcgaac gcacggcggc
     6361 ctcgacctcg ttgcgataga agccgccgga gcggtcgatc acgtcgtaca gcaccgccgc
     6421 cttgcgcgcg ttgcgcttgg cgaattcggc cacgccaccc tcgtcgagca tccacttcac
     6481 ggtcaggccg agcatgtacc agttccagct cggcggggtg ttgagcatcg actccgcctt
     6541 cagctggctg cggaagtcga agatgtcggc gcgcggctgg ccggcgcgtt ccagaagctc
     6601 gcggtccacg atcagcacgc cgatcccgac cgggcccagg ttcttctgcg cgcccgcgta
     6661 gatcgccccg aacctgcgga tgtccagcgg ctcggacgcg atcgaggagc tgaagtcggc
     6721 gatcaacggc acgctgccgg tgtccggaag ctcgcggaac tcgaccccgt ggatggtctc
     6781 gttggcggtg atgtgcacgt acgccgcgtc cggcgacagg ttccaggacg cgcgcgcggg
     6841 gatcgagcgg aagccatcgg cctcgctgct ggcggcgacg ttggcggcgg catacggctt
     6901 ggcctgcttg agcgcggtct tgccccaatg gccggtcacc acgtagtcca ccggctgccc
     6961 ggcggcggcg aagttcagcg gcagcagcgc ctgggtggtg gtggcgccgc cggaggtgaa
     7021 cagcaccgcg tagtcgtccg ggatcgacag cagggtgcgc aggtcggcct cggtgcgctg
     7081 cgccaccgcg atgaactcgg ggccgcggtg gctgagctcg acgatcgacg cgccggcgtt
     7141 gccgaattcc agcatctccg cctgcgcctg gcgcagtacg ggctccggca gggtggcggg
     7201 accggcactg aagttgaagg cacgcgacat cggagggctc cacgacaagg ggttgggtcg
     7261 ccgcagtatg ccgcagtgca gcacgcgcgt ggcggcggtt gcgcacgtaa cctttacccc
     7321 cgccggggcg tactacgcaa tggcgcatcc gccaaacgcg cacctgcgta tcggccaatg
     7381 ccgccacccg ccacggagcc atccccatgc cacagcgcca ccccgccctc cccgctgacg
     7441 ccgacgcgat caccgaggag gcggtctacc gcgatcgccg ccggctgctg gcggcgttcg
     7501 ggctcctgcc cacgctgggc ctggcgggct gttcggaggc ggcgccgccg gcgtcgaagg
     7561 cggtggtgac gccggcgcag gcgaagtcgg ggttccgcac cgcagaggag cagacccgcg
     7621 agctggacgc caccacctac aacaatttct acgaattcgg caccaacaag gacgacccgg
     7681 cgaacgcgcc gaagacgctg cgcacgaagc cgtggtcggt ggcggtgggc gggcattgcg
     7741 cccggcccgg cacgctggcg ctggaggacc tgctgaaggg cttgccgtcg caggagcgga
     7801 tctaccggat gcgctgcgtg gagggctggt cgatggtgat cccgtggctg ggggtgccgc
     7861 tggcggacgt gctgaagcgc ttcgagccga cctcgaaggc gaaatacgtg gcgttcacca
     7921 gcctggcgga tccgcggcag atgccggggg tccgggtgcc gacactggac tggccgtacc
     7981 gcgagggcct gcgcatcgac gaagccatgc atccgctcac cctgctggcc acgggcatgt
     8041 acggcaaggc gctgccgcag cagaacggtg cgccgttgcg gttggtggtg ccgtggaaat
     8101 acgggttcaa gggcatcaag tcgatcgtgg cgatcacctt cgtggagcgg atgccgaaga
     8161 cgtcctggaa catgtcgcag ccatcggagt acgggttcta ttcgaacgtg aacccggcgg
     8221 tggaccatcc gcgctggagc cagaagagcg aacgccggat cgcgggcagc gccagcaagc
     8281 tgttcgccga gcgcatcccg acgcggccgt tcaatggcta tgccgagcag gtggcgtcgc
     8341 tgtatgcggg gatggatttg aagaagtggt tctgattcct cgcttcgcgc ctgcgcggcc
     8401 ggtgcgcctt tgcgcgacac gccgtgaata cccgctccgc gggcccggcc cgcgaccaca
     8461 gatcgcgggc gttcggctgc gcgcgaagct gcgcttcgca agcgcttgtc tcacccatgc
     8521 aggctcatcg gcggcatcca tgccgccgaa ggtcgctcaa aggcgcaccg gccccacatg
     8581 cgctcactga ttcttccgaa gagcatcgtc caccttgccg cgctgacgcc gctggcgatc
     8641 ctgctgtggc aggcgtggag cgtggcgaag accggcggca acgcgctcgg tgcggacccg
     8701 gtggccgaga tcgaacatcg gctgggcctg tgggcgctgc gcttcctggc ggtcacgctg
     8761 gcgatcaccc cgctgcggca gctcaccggc tggaacgtgc tggtgcggtt ccggcggatg
     8821 ctcgggctgt acgcgttcgc gtacgcgacc ctgcatttcg cggcctacct ggggctcgac
     8881 ctgcgcgggt actggacgca ggtgttcgag gacatcgcca agcggccgta catcacggtc
     8941 gggttcaccg cgtggctgtt gctggtgccg ctggcggtca cctccaccag cggctggatc
     9001 aggcggctcg gccgcaactg ggcgcggctg cacatgctgg tatacccggt cgcggtgctg
     9061 gcggtgctgc acttctggtg gctggtgaaa tccgacctgc gcgaaccggc gctgtatgcg
     9121 gtgatcgtcg cgctgttgct gggctggcgg acctggaagc gctggaaacc tagaacttct
     9181 ctttcagcac gcgcttgatc tcgccctcga tcggctcctt gaacatcgcg gtcaggaaac
     9241 cgagcttggc gtgcacgtgc aggtcgttcg gctccagcat gatgtggccg tccaccccgg
     9301 agcgcgagaa gttgagggtg tcgccttccc agccgtaatc catgtcgaac ttctcggcga
     9361 gcttcttcgc cacttcctcg attgccttgc gcgccttggc cttgggcagg gagtggggat
     9421 ggcggatatc gatcttggac atggcaaccg gcttccagcg ggcaatggcg cattgtgccc
     9481 ggcgatccgg cgcacaacca gccgggccgg gcccgcacgc atgttagatt cgcgccgatg
     9541 aatgaattgc gtcttcgatt cggcgatggc gaccaacccg acctggtgct cggctccggc
     9601 gtgcatgcgc ttggccggct gccgaccggg ctgggcccgg tcgacgccga gcagccgtgg
     9661 ctgctgcagc tgtgcaacga ccgtcgcggg atctggctga cggtcgcgga cgacctgcgc
     9721 ggggtccacg tcaacggccg cccggtgcag cacgtggcga tgctgcgcgc cggcgacagc
     9781 atccatgtcg acggcaacga attgctgctc accgccgcca acgacgggcg cgcgctgccg
     9841 ccgccggcgg cccagcgcga cccgatcggc aacctgcggc tggtcctgcg cggtgtcggc
     9901 ggcgcccacc acggacgcag catcagcctg gagacgccgc gccggatcgg ccgcgcggcc
     9961 gacgccgaca tccgcatcga aggcccgggc atcgccgaac gccacgccct gctggatgcg
    10021 gtgaacggcc aggcagtcct gcgcgaagcc ggtgccgacg tgctggtcaa cggccagcgg
    10081 gtgcgccagg cggtgctgcg cgcaggcgac cagatcgcat tcgacgtgca gcaccggttc
    10141 gtgctggaag gcccgccgcc ggcgaccgcg gcgccggcgg ccggacgccc gcgcgccgct
    10201 gccgacccgg atgcagcccc cgaaccgcca cggcgctcgt ggctgcaacg gatgccgtgg
    10261 ctgctggtcg cggcgatcct gctggcggcc gcgctgtcgg cgctgctgct gttcggcgcg
    10321 cgttagcagc aa
//
LOCUS       DAORUK010000067        10250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_6892_length_10250_cov_17.996763, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000067 DAORUK010000000
VERSION     DAORUK010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10250
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6892_length_10250_cov_17.996763"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PK818_06025"
     CDS             <1..266
                     /locus_tag="PK818_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HQY82325.1"
                     /translation="ALDFLLANGFIHKLESINAFVACHHPDSAQHSVPFLICNRCHSA
                     TELEDASIVATLDAAARALGFAPQAQTLEVHGLCAKCAIAERP"
     gene            complement(324..2513)
                     /locus_tag="PK818_06030"
     CDS             complement(324..2513)
                     /locus_tag="PK818_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY82326.1"
                     /translation="MPAHPRPRLLAIALLLALPAFAAEPPQQAQHDRQPKDLAAVEVR
                     ATPLADTAESLATPVDVLAGSKLDEAKAGTLGETVARLPGVQSSNFGPGVGRPIIRGL
                     DGARVQVLSDGLGSGDVSTVSVDHAVSIEPFLANQIEVLKGPATLLYGSGAIGGAVNV
                     VDGRIPESIADVPFSGRAELRRGSVNDEATGMVRIDGNAGAFAFHFDALHRETGDYAI
                     PGYPESAALMAEHGETPDPDEAGILRNSAVRTDSAALGLSWIGARGFIGGSYSLFNTA
                     YGIPGGHAHGGEEDEHEGEHAEEEAEEHGDGDVSILMDQRRSELRAGIDNVGPFQSMR
                     LKLADTDYTHTEFEGEAVGTVFHNRSREARAELVHVPVAGWKGAFGVQASKRDFDAIG
                     DEAFVPASRSYDTGLFWIGKRSFDTARGALQVDLGVRHDDNRIDVDNVVTAAPDRHVH
                     TTSASAGFGWKASEALHLSLGLDRAQRAPGAEELYSNGLHVATGSFEFGDATLGVETA
                     RRIELGLHLHHGPVEVQLSAWQARYDDFVYLAGTDAQVDGAPLRVWRQDDARFHGLEA
                     KLDWDIAGTDAGVWSLNVFGDIVRGRLVADAGSTALQVFEVEHGDHVDAVTARVANGG
                     NLPRIVPARVGATLRWERERWRASLGAVRYAKQDDVAAYETATPGYTLVDAHVAWHID
                     TKSGREIELFVDANNLSDQEARPHTSFLKDVVPLPGRNVVLGVRAFF"
     gene            2585..2995
                     /locus_tag="PK818_06035"
     CDS             2585..2995
                     /locus_tag="PK818_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HQY82327.1"
                     /translation="MSPQQRHRLLDRLGATGSLLCALHCALLPVLIALLPSMGFAGLM
                     GDGIEEGFVLFASLLGLFTIVQGYRRHRAVRALWLLVPGLAALWLGIGHEPLHHDAIL
                     HAVTMTFGGTLVGLAHLANLRLSHGHVHDATCAH"
     gene            3066..3245
                     /locus_tag="PK818_06040"
     CDS             3066..3245
                     /locus_tag="PK818_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HQY82328.1"
                     /translation="MGKGDRKTAKGKRYNSSYGNARSHATTKAAGSAAAPVVKKAATR
                     TVAKAPAKKVVAKKA"
     gene            complement(3303..4499)
                     /locus_tag="PK818_06045"
     CDS             complement(3303..4499)
                     /locus_tag="PK818_06045"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HQY82329.1"
                     /translation="MRRCILLAALLIAANGAHAQDAARRLAQDAVIVDTHVDAPGILM
                     DKWADLGIEARDREFDYPKARAGGLDVAFMSIYTSHGQDTDGSAWQVANRMIDGVHAL
                     VQRHPGRFALLTSPADVARLREGGRVLLPLGMENGAPLGDKLANVQFFFDRGVRYITL
                     AHSGNNRLADSSYARDRQWNGLSPFGRQVVAEMNRLGIMVDVSHLGDASAKEAIALSR
                     VPVIASHSAFRHFTPGFERNISDEIARLVAAKGGVVQVPFGTAFINPDSAADTQAHFR
                     AINAFNTRNAELKAQGQPEQDRAAFDKAWAAAHPVRSSTLAQVLEQIDHGVKLLGIDH
                     VGIGSDFDGVDGELPAELKTVADFPNLVAGLQARGYREADIRKILGGNLLRAWAEVEA
                     GASRQP"
     gene            complement(4521..5744)
                     /gene="hutI"
                     /locus_tag="PK818_06050"
     CDS             complement(4521..5744)
                     /gene="hutI"
                     /locus_tag="PK818_06050"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HQY82330.1"
                     /translation="MTMQPRWDGLLLGATLATLDADAGYGLVADAALGWKDGVITYAG
                     SRAGLPGEIDGLSDEVIEAEGVVTPGLVDCHTHVVFAGDRAAEFELRLQGASYEQIAR
                     AGGGILSTVRAVRAASEDELFRQSEPRIAALLRDGVTSLEIKSGYGLDFDNERKMLRV
                     ARALGERFGITVRTTCLAAHALPPEFAGDADGYIDAAIGWLPRLHAEGLVDAVDAFCE
                     GIGFSPAQTRRMFEAARALGLPVKLHADQLSDLGGAALAAESGGLSADHIEFTSEDGV
                     RAMALHGTVAVLLPGAFHVLRETKLPPLDLLRQHRVSMAVATDCNPGTSPLLSLRQAM
                     QLACTHFRLTPEEALRGTTVNAAKALRLADRGVLRAGLRADFALWRIRHPAELCYWLG
                     GTLLHASYAGGHRLT"
     gene            5782..7011
                     /locus_tag="PK818_06055"
     CDS             5782..7011
                     /locus_tag="PK818_06055"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HQY82331.1"
                     /translation="MRNHATATPRGWALPGIANLHSHAFQRAMAGMAERQTDPRDSFW
                     TWRETMYAMAARFDPGLLQAVAAQLYVEMLEAGYTTVCEFHYLHHAADGTPHAQPDAM
                     SQALLRAARETGIRITLLPVLYMTGGFDGRALSERQRRFGHRVDAYLRLHETLRANAG
                     DTVRIGCALHSLRAVPAAAMGEVLAGLPADARIHIHVAEQTAEVDECVAVRGARPVQW
                     LLDNAAVDARWTLVHATHLDAAEVQGIARSGATVAICTTTEANLGDGLFPLRSFLDAG
                     GRWGIGSDSHISVSPVEELRWLEYGQRLHARRRNIASSADSPSVGAALLQGVLGSAAD
                     SSGFAADDRIVLDAQAPILAGASADDLIDRWIFSGNASLVRDVVVAGVRVVEDGRHRD
                     REAIAARYREAMRQLLS"
     gene            complement(7062..7565)
                     /locus_tag="PK818_06060"
     CDS             complement(7062..7565)
                     /locus_tag="PK818_06060"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HQY82332.1"
                     /translation="MILCRRIDSPIGPLMLAADDSGLRHIEFRDNSHPADRRDWHGGS
                     NDILQATEAQLGEYFAGQRQAFELPLAPQGTPFQLQVWEGLARIPFGATISYAQLAQR
                     IGNPAGTRAVGAANGRNPLPIVLPCHRVIGADGAMTGFGGGVPVKEFLLRLEGSLPAG
                     QAGLFAL"
     gene            complement(7562..8785)
                     /locus_tag="PK818_06065"
     CDS             complement(7562..8785)
                     /locus_tag="PK818_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HQY82333.1"
                     /translation="MPPRHLVALLFLLLAMVGCASLPEPASRHAVVLVSIDGLPADVP
                     GSGRMPALDAIAREGTRAEWVNPSYPTLTFPNHYTLVTGLRPDHHGIVHNNMRDPALG
                     RFVSKDASAHDGRWWGGEPIWATLQRQGGIAATMFWPGSEADIAGERPRHWQRFNGAV
                     PVQARVQQVLAWLDLPPARRPRLVTLYLEQYDVAAHAAGMDSDRAMRALATLDAGLAQ
                     LRDGLRARGLGDNVDLIVLSDHGMADVRGEDVRYIDDLVPADAILPEYGGQLMGFAPR
                     PGREREVEAAVLGRHDHFQCWRKGELPAGWHYGRNPRVPAIVCQADHGWRVWMRHWPA
                     QAALKGEHGFAPEDPGMRAAFVAAGPSFRAGTQLPAFDNVDVYPLLAHLLGIAPAPND
                     GDLAPLRPALRDARR"
     gene            8999..>10250
                     /gene="hutU"
                     /locus_tag="PK818_06070"
     CDS             8999..>10250
                     /gene="hutU"
                     /locus_tag="PK818_06070"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HQY82334.1"
                     /translation="MSSRHDPSRSIRAPRGNTLSCRSWLTEAPFRMLQNNLDAEVAED
                     PASLVVYGGIGRAARDWDCYDRIIETLKTLGDDETLLVQSGKPVGVFPSHADAPRVLI
                     ANSNLVPHWATWEHFNELDRKGLMMYGQMTAGSWIYIGSQGIVQGTYETFVEMGRQHY
                     GGSLQGKWILTAGLGGMGGAQPLAASLAGACSLNIECRQSSIDFRLKTRYVDEQATDL
                     DDALARIEKCTQAGEAKSIALLGNAAEILPELVRRGVRPDCVTDQTSAHDPVHGYLPI
                     GWTVEQWLAEQQANPEKVRDAAKQSMRVHVEAMLAFHAAGIPTVDYGNNIRQMAFDVG
                     CKNAFDFPGFVPAYVRPLFCRGIGPFRWVALSGDPEDIYKTDAKVKELIPDDAHLHRW
                     LDMARERISFQGLPARICWVGLGLR"
BASE COUNT         1501 a         3571 c         3612 g         1566 t
ORIGIN      
        1 gcgcgctgga tttcctgctg gccaacggct tcatccacaa gctggaatcg atcaatgcgt
       61 tcgtggcctg ccaccatccg gacagcgcgc agcactcggt gccattcctg atctgcaacc
      121 gctgccattc ggccaccgaa ctggaggacg ccagcatcgt ggccacgctg gatgcggcgg
      181 cgcgggcgct gggattcgcc ccgcaggcgc agacgctgga agtgcacggc ctgtgcgcga
      241 agtgcgcgat cgccgagcgc ccatagggcg gggcaagccc cgctgcggtt gcgcggtagc
      301 gggccaacgc ccgcccgctc ggatcagaag aacgcgcgca cgccgagcac cacgttgcgc
      361 cccggcaacg gcaccacgtc cttcaggaac gaggtgtgcg gccgtgcttc ctggtcgctg
      421 agattgttgg cgtccacgaa cagctcgatc tcgcggccac tcttcgtatc gatgtgccag
      481 gcgacgtgtg catccaccag cgtgtagccg ggcgtcgcgg tttcgtaggc ggccacgtcg
      541 tcctgcttcg cgtaacgcac cgcacccagc gaagcgcgcc agcgttcgcg ctcccagcgc
      601 agggtggcgc cgacgcgggc gggcacgatg cgcggcaggt tgccgccgtt cgcgacgcgc
      661 gcggtgaccg cgtccacgtg gtcgccgtgc tcgacctcga acacctgcag cgcggtgctg
      721 ccggcgtcgg cgaccagccg gccgcgcacg atgtcgccga acacgttgag cgaccacacg
      781 ccggcgtcgg tcccggcgat gtcccagtca agcttggcct caagtccgtg gaagcgcgcg
      841 tcgtcctggc gccacacgcg cagcggagcg ccatcgactt gtgcatcggt gccggcgagg
      901 tagacgaagt cgtcgtagcg cgcctgccag gcggacagct ggacctcgac cggcccgtgg
      961 tgcaggtgca ggccgagttc gatccgccgc gcggtttcca cgcccagggt ggcgtcgccg
     1021 aattcgaagc tgccggtggc cacgtgcagg ccgttggaat acagctcctc cgcgcccggt
     1081 gcgcgctgcg cgcgatccag gcccaggctc aggtgcaggg cttcgctggc cttccagccg
     1141 aaaccggcgg acgcgctggt ggtatgcacg tggcggtccg gcgccgcggt caccacgttg
     1201 tcgacgtcga tgcggttgtc gtcgtggcgc acgccgaggt cgacctgcag tgcgccgcgc
     1261 gcggtgtcga agctgcgctt gccgatccag aacaggccgg tgtcgtagct gcgcgaggcg
     1321 gggacgaagg cctcgtcgcc gatcgcgtcg aaatcgcgtt tgctggcctg caccccgaac
     1381 gcacccttcc agccggcgac cggcacgtgc accagttccg cgcgtgcctc gcggctgcgg
     1441 ttgtggaaca cggtgccgac cgcctcgccc tcgaattcgg tgtgcgtgta gtcggtatcc
     1501 gccagtttca ggcgcatcga ctggaatggg ccgacgttat cgatgcccgc gcgcagttcg
     1561 ctgcggcgct ggtccatcag gatgctcacg tcgccgtcgc cgtgctcctc ggcttcctct
     1621 tccgcgtgct cgccttcgtg ttcgtcttcc tcaccgccat gcgcatgccc gcccgggatg
     1681 ccgtaggcgg tgttgaacag gctgtagctg ccgccgatga agccgcgtgc gccgatccac
     1741 gacagcccga gcgccgcgct gtcggtgcgc accgcgctgt tgcgcaggat cccggcctcg
     1801 tcggggtccg gggtctcgcc gtgttcggcc atcagcgccg cgctttccgg atagccgggg
     1861 atggcgtagt cgccggtttc gcgatgcagc gcgtcgaaat ggaaggcgaa cgcgccggcg
     1921 ttgccatcga tgcggaccat gccggtggct tcgtcgttga cgctgccgcg gcgcaactcg
     1981 gcacgaccgc tgaacggcac gtcggcgatg gattcgggga tgcggccgtc gaccacgttg
     2041 accgcgccgc cgatcgcgcc gctgccgtac agcagggtgg ccgggccctt cagcacttcg
     2101 atctggttgg ccaggaacgg ctcgatgctg accgcgtggt cgacgctgac ggtggacacg
     2161 tcgccggagc ccaggccgtc gctcagcacc tgcacgcgcg cgccgtcgag gccgcggatg
     2221 atcggacggc cgacgccggg gccgaagttg gacgactgca cgcccggcag ccgcgccacg
     2281 gtttcgccca gggtgccggc cttggcttcg tccagcttgc tgccggccag cacgtccacc
     2341 ggcgtggcca ggctctcggc ggtgtccgcc agcggtgtgg cgcggacttc gaccgcggcc
     2401 aggtccttcg gctgccggtc gtgctgggcc tgttgcggcg gctcggcggc aaacgcgggc
     2461 agggccagca acagggcgat ggcgagcagg cgagggcggg gatgggcagg catcgggacg
     2521 ggctaggcag gtgtagtgga atgttatact ataactatgc tggaaaccgc cacggctgga
     2581 agtcatgtcc ccgcagcaac gccaccgcct gctcgaccgc ctcggcgcga ccggctccct
     2641 gctgtgcgcg ctgcattgcg ccttgctgcc ggtgctgatc gcgctgctgc cctcgatggg
     2701 gttcgccggc ctgatgggcg acgggatcga ggagggcttc gtcctgttcg cgagcctgct
     2761 gggcctgttc accatcgtcc agggctatcg ccggcaccgc gcggtgcgcg cgctgtggct
     2821 gctggtgccg gggctggccg cgctgtggct ggggatcggc cacgagccgc tgcaccacga
     2881 cgcgatcctg cacgcggtga ccatgacctt cggcggcacc ctggtcggcc tggcccacct
     2941 cgccaacctg cggctcagcc acggccacgt gcacgacgcc acgtgcgcgc attgaccgcg
     3001 tgttagaatc cacgccctcg cgcgcggcgc gaaccacatt ctcgaatcga tccaggagca
     3061 acacgatggg caagggcgac cgcaagacgg ccaagggcaa gcgctacaac tccagctacg
     3121 gcaatgcgcg ttcgcacgcg accaccaagg ccgccggcag cgctgccgcg ccggtggtga
     3181 agaaggccgc gaccaggacc gtcgccaagg cgccggccaa gaaggtcgtg gcgaagaagg
     3241 cctgacccgc cggatcccgc tgttgtagag cgaaagcccc gccccggcgg ggctttcgca
     3301 tttcagggct ggcgtgatgc gccggcttcg acctcggccc acgcgcgcag caggttgccg
     3361 ccgaggatct tgcggatgtc ggcttcgcga tagccgcgcg cctgcaggcc ggcgaccagg
     3421 ttcgggaagt cggccaccgt cttcagttcg gccggaagtt cgccgtcgac gccgtcgaag
     3481 tcggagccga tgccgacgtg gtcgatgccg agcagcttca ccccatggtc gatctgctcc
     3541 agcacctgcg ccagcgtgct gctgcgcacc gggtgcgcgg cggcccaggc cttgtcgaag
     3601 gcggcgcggt cctgttccgg ctggccctgt gccttcagtt ccgcgttgcg cgtgttgaac
     3661 gcgttgatcg cgcggaaatg cgcctgggtg tcggcggcgc tgtcggggtt gatgaaggcg
     3721 gtgccgaacg gaacttgcac cacgccaccc ttcgccgcca ccagccgggc gatctcgtcg
     3781 ctgatgttgc gttcgaagcc gggggtgaaa tggcggaacg cggagtggct ggcgatcacc
     3841 ggtacccggc tgagcgcgat cgcttccttc gccgaggcgt cgccgaggtg ggagacatcg
     3901 accatgatcc cgagccggtt catttccgcc accacctggc ggccgaatgg gctcagcccg
     3961 ttccactgcc tgtcgcgcgc gtaggacgaa tcggcgaggc ggttgttgcc gctgtgggcc
     4021 agggtgatgt agcgcacgcc gcggtcgaag aagaactgca cgttcgcgag tttgtcgccc
     4081 agcggggcgc cgttctccat ccccagcggc agcagcacgc ggccgccttc gcgcaggcgc
     4141 gccacgtcgg ccggcgaggt cagcagcgcg aacctgcccg gatgccgctg caccagcgcg
     4201 tgcacgccat cgatcatgcg gttggcgacc tgccaggcgc tgccgtcggt gtcctgcccg
     4261 tgggaggtgt agatcgacat gaaggcgaca tccaggccgc ccgcgcgcgc cttcggatag
     4321 tcgaattcgc ggtcccttgc ctcgatcccg aggtcggccc acttgtccat caggatgccc
     4381 ggcgcatcga cgtgggtatc gacgatgacc gcgtcctgcg ccagccggcg cgcggcgtcc
     4441 tgcgcgtggg cgccgttggc ggcgatgagc agggcggcaa gcaggatgca acgacgcatg
     4501 ttcggcactc cgtggtcggt tcaggtgagg cggtggccgc cggcatacga ggcgtgcagc
     4561 agggtgccgc ccagccagta gcagagttcg gcgggatggc ggatgcgcca cagcgcgaag
     4621 tcggcgcgca ggcccgcgcg caggacgccg cgatcggcca ggcgcagcgc cttggcggcg
     4681 ttcaccgtcg tcccgcgcag ggcttcctcc ggggtcagcc ggaagtgcgt gcaggccagt
     4741 tgcatcgcct gacgcagcga aagcagcggc gacgtgcccg gattgcaatc ggttgcgacc
     4801 gccatcgaca cccggtgctg gcgcagcagg tcgaggggtg gcagtttcgt ttcgcgcagc
     4861 acgtggaatg cgccgggaag cagcacggcg accgtgccgt gcagcgccat cgcgcggacg
     4921 ccgtcctcgc tggtgaattc gatgtggtcg gcggacagtc cgccggattc cgcggccagc
     4981 gccgcgccgc cgaggtcgct cagctggtcc gcatgcagct tcaccggcag ccccagcgcg
     5041 cgtgcggcct cgaacatccg ccgggtctgc gccgggctga agccgatgcc ttcgcagaac
     5101 gcgtccactg catccaccag gccttccgcg tgcaggcgcg gcagccagcc gatggcggca
     5161 tcgatgtagc cgtccgcatc gcccgcgaat tccggcggca gcgcatgcgc ggcgaggcag
     5221 gtggtgcgca ccgtgatccc gaagcgttcg cccagcgcgc gcgccacccg cagcatcttg
     5281 cgttcgttgt cgaagtccag gccatagccg gacttgatct ccaggctggt gacgccgtcg
     5341 cggagcaggg ccgcgatgcg cggctccgac tgcctgaaaa gctcgtcttc gctggccgcg
     5401 cgcactgcgc gcacggtcga caggatgccg ccgccggcgc gcgcgatctg ttcgtagctc
     5461 gcgccctgca ggcgcagctc gaactcagcg gcacggtcgc cggcgaacac cacgtgggta
     5521 tggcagtcga ccagccccgg ggtaaccacg ccctcggcct cgatgacttc gtcggatagc
     5581 ccgtctattt cgcccggcaa gcctgcgcgc gatccggcat acgtgatcac gccatccttc
     5641 cagcccagtg cggcgtccgc gaccaggccg tagccggcgt ccgcgtccag cgtggccagt
     5701 gtggcgccaa gcagcagccc gtcccatcgc ggttgcatcg tcatgcggcg attgtaagcc
     5761 gcgcccgcat acaatccgcc gatgcgaaac cacgccaccg ccaccccgcg cggctgggcc
     5821 ttgcccggca tcgccaacct gcattcgcat gcgttccagc gcgcgatggc gggcatggcg
     5881 gagcgccaga ccgatccacg cgattcgttc tggacctggc gcgaaaccat gtacgcaatg
     5941 gccgcgcgct tcgatcccgg gctgctgcag gcggtggccg cgcagctgta cgtggagatg
     6001 ctcgaagccg gctacaccac ggtctgcgag ttccactacc tgcaccacgc ggcggacggc
     6061 accccgcatg cgcaacccga cgcgatgtca caggccttgc tgcgcgcagc ccgcgagacc
     6121 ggcatccgca tcaccctgct gccggtcctg tacatgaccg gcggcttcga cggtcgcgcg
     6181 ctgtccgagc gccagcggcg tttcggccac cgggtcgatg cctacctgcg attgcacgag
     6241 acactgcgcg cgaatgctgg cgacacggtg cgcatcggct gcgcgctgca cagcctgcgt
     6301 gcggtgccgg cggcggcgat gggcgaggta ctggcggggt tgccggccga cgcgcgcatc
     6361 cacatccacg ttgccgagca gaccgccgaa gtcgacgaat gcgtggcggt gcgcggcgcg
     6421 cgcccggtgc agtggctgct ggacaacgcg gcggtggatg cgcgctggac gctggtgcat
     6481 gccacccacc tcgacgcggc cgaagtgcag ggcatcgccc gcagcggcgc gacggtggcg
     6541 atctgcacca ccaccgaagc caacctcggc gatggcctgt tcccgctgcg cagcttcctg
     6601 gatgccggtg ggcgctgggg catcggttcg gattcgcaca tctcggtctc gccggtggag
     6661 gaactgcgct ggctggaata cgggcaacgg ctgcacgcgc gccgccgcaa catcgccagc
     6721 agcgcggatt cgcccagcgt cggcgccgcc ctgctgcagg gcgtgctggg aagtgccgcc
     6781 gacagcagcg gattcgccgc cgatgaccgc atcgtgctgg acgcgcaggc accgatcctc
     6841 gccggcgcca gcgcggacga cctcatcgat cgctggatct tcagcggcaa cgccagcctg
     6901 gtgcgcgatg tcgtcgtcgc cggcgtgcgc gtggtcgagg acggccgcca ccgcgatcgc
     6961 gaggcgatcg ccgcgcgcta ccgcgaggcg atgcggcagt tgctgtcgta ggcgcggcgc
     7021 aagcctggaa ctggcatcgc atgtcgcggc ttgcgccgcc cctacagcgc gaacagcccc
     7081 gcctgcccgg cgggcaatga cccttccagc cgcagcagga attccttgac cggcacgccg
     7141 ccgccgaagc cggtcatcgc gccgtctgcg ccaatcactc ggtggcaggg cagcacgatc
     7201 ggcagcgggt tgcggccatt ggcggcgccc accgcgcggg tgccggcggg attgccgatg
     7261 cgctgcgcga gttgcgcgta gctgatcgtc gcgccgaacg ggatgcgcgc gagtccttcc
     7321 cagacctgca actggaacgg cgtgccctgc ggggccagcg gcagctcgaa cgcctggcgc
     7381 tggccggcga agtattcgcc gagttgcgcc tcggtcgcct gcaggatgtc gttgctgccg
     7441 ccatgccagt cgcgccggtc cgcaggatgg ctgttgtcgc ggaattcgat gtggcgcagg
     7501 ccgctgtcgt ccgccgccag catcagcggt ccgatcgggc tgtcgatgcg ccggcacagg
     7561 atcatcgccg cgcatcccgc agcgcgggcc gcagcggcgc gaggtcgccg tcgttcggcg
     7621 cgggcgcgat gcccagcagg tgcgcgagca gcggatacac atcgacgttg tcgaacgccg
     7681 gcagttgggt gcccgcgcgg aatgacggac ccgccgcgac gaacgccgcg cgcatgcctg
     7741 gatcctcggg cgcgaagcca tgctcgccct tcagcgcggc ctgcgcaggc cagtggcgca
     7801 tccagacgcg ccatccgtgg tcggcctggc agacgatggc agggacgcgc ggattgcgac
     7861 cgtagtgcca gcccgccggc agttcgccct tgcgccagca ctggaagtgg tcgtggcggc
     7921 caagcacggc ggcctcgacc tcgcgctcgc ggccggggcg cggggcgaag cccatcagct
     7981 ggccgccgta ctcgggaagg atcgcgtccg cgggcaccag gtcgtcgatg tagcggacgt
     8041 cctcgccgcg cacgtcggcc atgccgtggt cggacagcac gatcaggtcc acgttgtccc
     8101 cgagtccgcg cgcgcgcagt ccatcgcgca actgtgcgag cccggcatcg agggtggcca
     8161 gcgcccgcat cgcccggtcg gaatccatgc cggcggcgtg cgcggccacg tcgtactgct
     8221 ccaggtacag ggtgaccagt cgtggtcgcc gcgccggcgg caggtcgagc caggccaaca
     8281 cctgctgcac gcgcgcctgc accggcactg cgccgttgaa ccgctgccag tggcgcgggc
     8341 gctcgccggc gatgtccgct tccgagccgg gccagaacat ggtggccgcg atcccgccct
     8401 gccgctgcag ggtggcccag atcggctcgc caccccacca gcggccgtca tgtgcgctgg
     8461 cgtccttcga cacgaaccgt cccagtgccg ggtcacgcat gttgttgtgg acgatgccgt
     8521 ggtggtccgg gcgcaagccg gtcaccagcg tgtagtggtt ggggaaggtc agggtggggt
     8581 aactcgggtt cacccactcg gcgcgggtgc cttcgcgcgc gatcgcgtcc agcgccggca
     8641 tccgcccgct gccgggcaca tcggccggca ggccgtcgat cgagaccagc accaccgcat
     8701 gccgggacgc aggctccgga aggctggcgc atcccaccat ggcgagcagg aggaaaagca
     8761 gggcgacaag atggcgtggc ggcataagca gatgatagcc aaacggcgtt gtgcgcctgc
     8821 gcatcgtgtc tgacgattgc gagggccgtg gcggttgccg ctgccgcgaa tgtcccccgg
     8881 ccttcgcctg cggctgcggc ctcctccttg atttcgcggc agcggcaacc gccacgtccc
     8941 accgaattcc tcgacccccg atgcgataat cgcggcttac ccgcaggagc cgcatccgat
     9001 gtccagccgc cacgatccgt cccgcagcat ccgcgcgccg cgcggcaaca cgctcagctg
     9061 caggtcctgg ctgaccgaag cgccgttccg catgctgcag aacaacctgg atgcggaagt
     9121 ggccgaggat ccggcgtcgc tggtggtgta cggtggcatc ggccgcgccg cgcgcgactg
     9181 ggactgctac gaccggatca ttgaaacgct gaagaccctg ggcgacgacg aaaccctgct
     9241 ggtgcagtcg ggcaagccgg tcggcgtgtt ccccagccat gccgacgcgc cgcgcgtgct
     9301 gatcgccaat tccaacctgg tgccgcactg ggcaacctgg gagcacttca acgaactcga
     9361 caggaagggt ttgatgatgt acggccagat gaccgccggc agctggatct acatcgggtc
     9421 gcagggcatc gtgcagggca cctacgaaac cttcgtggaa atgggccgcc agcactacgg
     9481 cggttcgctg caaggcaagt ggatcctcac cgccggcctg ggtggcatgg gcggcgcgca
     9541 gccgctggcg gcgtcgctgg ccggggcctg cagcctcaac atcgaatgcc gccagagcag
     9601 catcgatttc cgcctgaaga cccgctatgt cgatgagcag gcgaccgacc tcgacgatgc
     9661 gctggcccgc atcgaaaaat gcacgcaggc cggcgaggcc aaatccatcg cgctgctggg
     9721 caatgccgcg gagatcctgc cggagctggt gcgccgcggc gtgcgcccgg actgcgtgac
     9781 ggaccagacc agcgcgcacg acccggtgca cggctacctg ccgatcggct ggacggtgga
     9841 gcagtggctg gcggaacagc aggccaaccc ggaaaaggtg cgcgacgcgg ccaagcagtc
     9901 gatgcgcgtg cacgtggagg cgatgctggc cttccatgcg gcgggcatcc ccaccgtcga
     9961 ctacggcaac aacatccgcc agatggcgtt cgatgtcggc tgcaagaacg ccttcgattt
    10021 ccccggcttc gtgccggcct atgtgcgccc gctgttctgc cgcggcatcg gcccgttccg
    10081 ctgggtggcg ctttcgggcg acccggaaga catctacaag accgacgcga aggtgaagga
    10141 gctgatcccg gacgacgccc acctgcaccg ctggctggac atggcgcgcg agcgcatcag
    10201 cttccagggc ctgccggcgc ggatctgctg ggtgggcctg ggcctgcgcc
//
LOCUS       DAORUK010000068        10185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_6958_length_10185_cov_20.297828, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000068 DAORUK010000000
VERSION     DAORUK010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10185
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6958_length_10185_cov_20.297828"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PK818_06075"
     CDS             <1..188
                     /locus_tag="PK818_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82335.1"
                     /translation="LQGMADRFHLLPYGLALVLMFIGVKMLLIDVYKIPITLSLAVVA
                     ATIGTTIALSLLRPQKA"
     gene            251..1018
                     /locus_tag="PK818_06080"
     CDS             251..1018
                     /locus_tag="PK818_06080"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014648951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQY82336.1"
                     /translation="MSTPLPADAQRQHDRRRLRRALNASLAFVLVLAACFAAQHRFDW
                     TWLAVAPLTTQGLLGLLGAPLLHGSAGHLVANASALLLLGTLAGTTYPKATLRSLPLL
                     WLGSGLVAWVLGQPGSHHLGASGVTHGLGFLVFVLGLLRRDRAAIAAGMLAFMFYGGM
                     LMTILPREAGVSWESHLGGALAGVVAAFLFRRSDPQPPRRKYSWEIEEELAREQAARE
                     RDMYERHAPEDVPVLWQRDGDAPGERGKVLPFRRPGD"
     gene            complement(1091..1732)
                     /locus_tag="PK818_06085"
     CDS             complement(1091..1732)
                     /locus_tag="PK818_06085"
                     /inference="COORDINATES: protein motif:HMM:NF021511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HQY82337.1"
                     /translation="MSAPAIAQPPGHFERWLLVGGVLLALGVSFVVASDRLTWFLEVI
                     WAMVGLALIAWKWQRFPLTRLLCWLLALHALVLIHGGAYTYSLTPGGFMLQDLLGTER
                     NPWDRLGHWMQGFVPAILARELLLRLTPLRRGGWLVYLVLAAALSFSAFFELIEWWAA
                     LVYGADADAFLATQGDQWDTQWDMFLCLCGAATSLLLLSRRHDRQLGAQVALP"
     gene            complement(1729..2439)
                     /locus_tag="PK818_06090"
     CDS             complement(1729..2439)
                     /locus_tag="PK818_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HQY82338.1"
                     /translation="MGRSVAKPHHERHRSEHIGWLRAAVLGANDGLISTSSLLVGVAA
                     AAASPQTVLVTGVAALVAGALSMAAGEYVSVSSQADTEAADTAKERRELEELPEAELK
                     ELTHIYVQRGLDPALAHQVAVQLTAHDALGAHLRDELGIAGHAIARPVQAALSSAAAF
                     AVGALPPLLLAWAWRGAGLQAALAVSTLLLLAVLGALAGWLGGASMVRGALRVATWGA
                     LALGATAAIGSLFGASLA"
     gene            2533..3558
                     /locus_tag="PK818_06095"
     CDS             2533..3558
                     /locus_tag="PK818_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQY82339.1"
                     /translation="MPAMRAFPATALLAVLLAACAGAPVAPAPAVVVVPAPAPVKVGI
                     ALGGGAAKGFAHIGVIKMLEANGIKPVVVSGTSAGSVVGALYASGMDAYAMQEKAFAL
                     DEARIRDVSLFSGGVVKGQKLQDYVNELVGGRTIEKMRKPLAVVATNLANGERTVFVR
                     GNTGQAVRASSSIPGVFEAVAIGKAHYVDGGVVSPVPVDAARELGAGFVIAVDISSKA
                     SGIASPTSMLGNVNQSITIMGQKLGALELQRADIVIRPQVGDIGATDFEQKNRAILEG
                     ERAAQAALPQIRAKLASLQAARVASARQAAEAEAARQREAERKARCARQQGWLDTLRR
                     DPDCREG"
     gene            3598..4488
                     /gene="phhA"
                     /locus_tag="PK818_06100"
     CDS             3598..4488
                     /gene="phhA"
                     /locus_tag="PK818_06100"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017158567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HQY82340.1"
                     /translation="MNAKPARLENLHTDKGKVPVYATGIVEQPWDDYTAADHDVWRQL
                     YERQRQLLVGRASDEFLQAQGMMGMSPDRIPRFDELNRVLEAATGWTIVGVEGLLPEL
                     DFFEHLANRRFPVSWWIRRPDQLDYIEEPDLFHDLFGHVPLLMIPAFADYMQAYGKGG
                     VKAHGLGAEALANLTRLYWYTVEFGLIRQHDGLRIYGSGIVSSKGESIHCLESDAPNR
                     IGFDLERIMRTRYRIDSYQKTYFVIDSFEQLMDATRPDFTPIYARLAQQGSIPAGSVL
                     DTDAVFHRGSGEGWATDGDV"
     gene            complement(4603..5307)
                     /locus_tag="PK818_06105"
     CDS             complement(4603..5307)
                     /locus_tag="PK818_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82341.1"
                     /translation="MNPYLAFKLVHIALGCVALVAFWTAGIARKGGPLHVGAGKVYLL
                     AMAGLLAATVPMCWLAWFAGHQKIVVFLLYLLVITCTSTWLSWRAIRDRRDWARYVGP
                     AYRALMWLNLGAGAGIALFGLFVADRMQLIIVSFSLIGISTFASMRRFALQPPTEARW
                     WMRQHLRAMLGNGVATHIAFLSIGLPRLLPMLQGPVFQNLAWLAPLGVAAVAGVYLGR
                     KYLGAPGRAGRLPQRA"
     gene            5452..6546
                     /locus_tag="PK818_06110"
     CDS             5452..6546
                     /locus_tag="PK818_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQY82342.1"
                     /translation="MLAFLRTAFAPLNLAALLAIGGIGMSLRWLPGEMRSGAFTLLIA
                     YTCCLLLATFPQRRRWAQNAALLLMPLLALAAIAVAPRMGTAQILLVVWAAVVVMAWP
                     PLVVLAAIVAVDLAAFGVLRHAGHSNPVITTILYASFQGFAALTSHYARSAEEARDRL
                     ARTNADLLATRALLADAARDGERLRVARELHDVAGHKLTALRLNLRALQAAAPSPQLQ
                     LAEQLSAELLGDIRGVVHALRDAGGLDIDTALRALAAPYPRPALRLSIDEGVVVEDPA
                     LAETVLRTVQEALTNAARHGHAATLDVRLRRDGTWLWVEIEDDGRASLPLREGHGLTG
                     MRERIDARGGRLRIARGSRGGVRIEAGVPA"
     gene            6543..7184
                     /locus_tag="PK818_06115"
     CDS             6543..7184
                     /locus_tag="PK818_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017161012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQY82343.1"
                     /translation="MSATPVRVALADDQALVRAGLRALLQQQGIDIVFEADDGQALLD
                     ALHTAPVDIILSDIRMPGLDGIAALEALRARGDATPVLLLTTFDDAELLLRATEAGAQ
                     GFLLKDAAPDDLREAILRVARGETLLQPVSTDAVRARYRYHADDAPPRDTFGKREVAI
                     LRLIAGGYSNREIARSLFLAEGTVKNYVSEILDKLGTRDRTRAVLKAITLRII"
     gene            7248..8816
                     /locus_tag="PK818_06120"
     CDS             7248..8816
                     /locus_tag="PK818_06120"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HQY82344.1"
                     /translation="MKMARFDAWWRWARQWRQARLVDTIDRVAVLENVHDGGALGPRY
                     AFMIVMACGIAMLGLLQNSVAVIIGAMLISPLMGPIVQLGMGLATFELRGIRDSLRTL
                     LAGVALALAIAILIVWMSPLQASTSEILARTEPTFFDLLVAVFSGLAGAYATVTRKGE
                     TIVGVAIATALMPPLAVVGYGIALGNWTIAGGAAFLFMTNLLAIALSVTIVARLYGFG
                     GSDSPKQTAWQAGLIVASFVLLSVPLGLALKRIALQSQTELLVRSALEESAARASGRV
                     SALRVESGADGVAVEAVVLMPDHVPGMEATLQTQLAARLGRPVSVRVQEVLTADDASF
                     GRQQGALAELRRSVSALQDAESVRSNQQRARAEERAALQASLLAYLGRLQRSADGSRW
                     ELWVAAETRMPLARAFRIEAELEAARADGAPVLRVFPPLQALPAIALETTEADGPGPG
                     EAALRSLAIQAWALQRWRAGTVDARLASDDDAQAEAWTAALREALSARGITLGRVERS
                     SEGAPGLQLALPAP"
     gene            complement(8823..9356)
                     /locus_tag="PK818_06125"
     CDS             complement(8823..9356)
                     /locus_tag="PK818_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82345.1"
                     /translation="MPRMLPILLFAALPLSACAPATTTPAIGASAPAASPAPPPAAAP
                     AHAPPAAAADPAATPATATGASIRGAIRDGNRPPPALRICAHPVGGGAPTCTDSPAGS
                     GDYRLGVAPGRYYLMGWVQAGELALLAHASQIRCIRAPCPPDELIEVAVAPGADVDGI
                     DLSGGYLEVPAGWPAKP"
     gene            9420..>10185
                     /locus_tag="PK818_06130"
     CDS             9420..>10185
                     /locus_tag="PK818_06130"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014035428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HQY82346.1"
                     /translation="MTPTPISLTRFLIEEQRAGRISPDLRLLVEVVARACKSISIAVG
                     KGALGGVLGDALSAGEASINVQGEAQKKLDVLSNEILLEANAWGGHLAGLASEEMDHS
                     MPIPDAYPRGNYLLLFDPLDGSSNIDVNISVGTIFSVLRCPDGVTHPGDEHFLQPGTA
                     QVAAGYCTYGPSTMLVLTVGHGTHAFTLDREVGSFVLTTRGMRIPEETREFAVNMSNQ
                     RFWEAPMQAYVGDLLAGSEGPRGKDFNMRWVASMVAD"
BASE COUNT         1516 a         3657 c         3607 g         1405 t
ORIGIN      
        1 tgctgcaggg catggccgac cgcttccacc tgctgccgta cgggctggcg ctggtactga
       61 tgttcatcgg cgtgaagatg ctgttgatcg atgtctacaa gatcccgatc acgttgtcgc
      121 tggcggtggt ggcggcgacc atcggcacca ccatcgcgct gagcctgctg cgcccgcaga
      181 aggcgtagcc gcccaggccc ggggcggccg gcgggcttga atgccagccg gccgccccaa
      241 tccgttgcgg atgtccacgc cacttcccgc agacgcgcaa cgccagcacg accgccggcg
      301 cctgcgccgc gccctcaatg ccagcctggc cttcgtgctg gtgctggcgg cctgcttcgc
      361 cgcccagcac cgcttcgact ggacctggct ggcggtggcg ccgctgacga cgcagggcct
      421 gctcgggttg ctgggcgcgc cgctgctgca cggctcggcc gggcacctgg tcgccaacgc
      481 cagcgcgctg ttgctgctgg gcaccctggc cggcaccacc tacccgaagg cgaccctgcg
      541 ttcgctgccc ctgctgtggc tggggtcggg cctggtcgcg tgggtgctgg gccagccggg
      601 ctcgcaccac ctcggcgcca gcggcgtgac ccacggcctg ggtttcctgg tgttcgtgct
      661 gggcctgttg cgccgcgacc gcgcggcaat cgccgccggc atgctggcct tcatgttcta
      721 cggcggcatg ctgatgacga tcctcccgcg cgaggccggg gtgtcgtggg aatcgcacct
      781 gggtggcgcg ctggcgggcg tggttgccgc cttcctgttc cgccgcagcg acccgcagcc
      841 gccgcgccgc aagtacagct gggagatcga ggaggaactg gcccgcgagc aggccgcgcg
      901 cgagcgcgac atgtacgagc gccacgcgcc cgaagacgtg ccggtgctgt ggcagcgcga
      961 cggcgatgcg cccggcgaac gcggcaaggt gctgccgttc cgccgccccg gtgattgaac
     1021 ccggattccc ctgggagcgc cccatgggcg cgctggcgtg gcgtgcatgc atcgcgggca
     1081 tggcccgctc ctacggcaac gccacctgcg cacccagctg ccggtcatgc cggcgcgaca
     1141 gcagcaacag cgacgtcgcc gccccgcaca ggcacaggaa catgtcccac tgggtatccc
     1201 actggtcgcc ctgggtggcg aggaaggcat cggcgtccgc gccgtagacc agtgcggccc
     1261 accattcgat cagctcgaag aacgccgaga aactcagcgc ggcggccagc accaggtaca
     1321 ccagccagcc gccgcggcgc agcggggtca gccgcagcag cagctcgcgc gccaggatcg
     1381 ccggcacgaa gccctgcatc cagtggccca gccgatccca cggattgcgt tcggtgccca
     1441 gcaggtcctg cagcatgaag ccgccgggcg tcagcgagta ggtgtaggcg ccgccgtgga
     1501 tcagcaccag cgcatgcagg gcgagcagcc agcacagcag gcgggtcagc gggaagcgct
     1561 gccacttcca ggcgatcagt gccaacccga ccatcgccca gatcacctcc aggaaccagg
     1621 tcaggcgatc gctggcgacc acgaacgaca cccccagcgc cagcagcacc ccgccgacca
     1681 gcagccaacg ctcgaagtgg cccggcggct gcgcgatcgc cggcgcgctc atgccaggct
     1741 ggcgccgaac aggctgccga tcgccgcggt cgcgcccagc gccagcgcgc cccaggtcgc
     1801 cacccgcaac gcgccgcgga ccatcgaggc accgccgagc cagcccgcca gcgcacccag
     1861 caccgcgagc agcagcaggg tggacaccgc cagcgccgcc tgcaggcccg cgcctcgcca
     1921 tgcccatgcc agcagcagcg gcggcaaggc cccgaccgcg aacgcggccg ccgacgaaag
     1981 cgccgcctgc accgggcgcg cgatcgcatg cccggcgatg ccgagctcgt cgcgcaggtg
     2041 ggcgccgagc gcatcgtgtg cggtcagctg caccgccacc tggtgcgcca gcgccgggtc
     2101 gaggccgcgc tgcacgtaga tgtgggtgag ttccttcagt tccgcttccg gcaattcctc
     2161 aagctcgcga cgctccttgg ccgtatccgc agcctcggtg tccgcctgcg agctgaccga
     2221 gacgtactcg cccgcggcca tcgacagcgc gcccgccacc agcgcggcga cgccggtgac
     2281 cagcacggtc tgtgggctgg cggccgcagc cgccaccccg accagcagcg aactggtcga
     2341 gatcagccca tcgttggcgc ccagcaccgc cgcgcgcagc cagccgatgt gttcgctgcg
     2401 gtggcgttcg tggtgcggct tggcaacgct cctgcccatg catgtccccc ggatgtcgcg
     2461 gctagggtac cgccaccggt tctgcccggg ttgcaattgc cgcgcataag ctgcgcgtcc
     2521 ctgccggatt cgatgcccgc gatgcgcgcc ttccccgcga ctgccctgct tgccgtcctg
     2581 cttgccgcct gcgccggcgc gcccgtcgca ccggcacccg ccgtcgtggt ggtgccggca
     2641 ccggcgccag tcaaggtcgg catcgcgctg ggcggcggcg cggccaaggg cttcgcccac
     2701 atcggcgtga tcaagatgct ggaagccaac ggcatcaagc cggtggtggt gtccgggacc
     2761 agcgccggca gcgtggtcgg cgcgctgtat gccagcggca tggatgccta tgcgatgcag
     2821 gaaaaggcgt tcgcgctgga tgaagccagg atccgcgacg tcagcctgtt ctccggcggg
     2881 gtggtgaagg gccagaagct gcaggactac gtcaacgaac tggtcggcgg ccgcaccatc
     2941 gagaagatgc ggaagccgct cgccgtcgtc gccaccaacc tcgcgaacgg cgaacgcacc
     3001 gtattcgtgc gcggcaacac cggccaggcg gtgcgcgcat ccagttcgat ccccggggtg
     3061 ttcgaagcgg tcgcgatcgg caaggcgcac tacgtcgacg gcggcgtggt cagcccggtg
     3121 ccggtggacg ccgcgcgcga gctcggcgcc gggttcgtga tcgcggtcga catctccagc
     3181 aaggccagcg gcatcgccag cccgaccagc atgctcggca acgtcaacca gagcatcacc
     3241 atcatgggcc agaagctcgg tgcgctggag ttgcagcgcg ccgacatcgt gatccggccg
     3301 caggtcggcg atatcggcgc caccgatttc gagcagaaga accgcgcgat cctcgaaggc
     3361 gaacgcgccg cgcaggccgc gctgccgcag atccgggcga agctggcaag cctgcaggcc
     3421 gcgcgcgtag ccagcgcccg gcaggcagcg gaagccgagg ctgcgcgcca gcgcgaggcc
     3481 gaacgcaagg cgcgctgcgc ccgccagcag ggctggctgg acaccctgcg gcgcgacccg
     3541 gattgccgcg agggctgagc aggccgcgct gtccggcggc ggcgtagact tgcgcgcatg
     3601 aacgccaaac ccgcacgcct cgagaacctg cacaccgaca agggcaaggt gccggtgtac
     3661 gccaccggca tcgtcgagca gccctgggac gactacaccg ccgccgacca cgacgtctgg
     3721 cgccagctgt acgaacgcca gcgccagctg ctggtcggcc gcgcctcgga tgagttcctg
     3781 caggcgcagg gcatgatggg catgagcccg gaccggatcc cgcggttcga tgagctcaac
     3841 cgcgtgctcg aggccgcgac cggctggacc atcgtcgggg tcgagggcct gctgccggaa
     3901 ctggatttct tcgagcacct ggccaaccgc cggttcccgg tcagctggtg gatccggcga
     3961 ccggaccagc tggactacat cgaggaaccc gacctgttcc acgacctgtt cgggcacgtg
     4021 ccgctgctga tgatcccggc gttcgccgac tacatgcagg cgtacggcaa gggcggggtc
     4081 aaggcgcatg gcctcggcgc ggaggcgctg gccaacctca cccgcctgta ctggtacacg
     4141 gtcgagttcg gcctgatccg gcagcacgac ggcctgcgca tctacggcag cggcatcgtc
     4201 tccagcaagg gcgagtcgat ccactgcctg gagtcggatg cgccgaaccg gatcggcttc
     4261 gacctcgagc gcatcatgcg cacgcgctac cgcatcgaca gctaccagaa gacctatttc
     4321 gtgatcgaca gcttcgagca gctgatggac gccacccgcc ccgacttcac accgatctac
     4381 gcgcggctgg cgcagcaggg ttcgatcccg gccggcagcg tgctggacac cgatgccgtg
     4441 ttccaccgcg gcagcggcga gggctgggcc accgacggcg acgtctgagc atcgatgctg
     4501 gcgcaagtgc ggcgcgcgcg gtcggctgct ggcgcagcga catttcgggt cgcgtagcga
     4561 attgccgccg gccccgccga cgctgcattg gacccgcgcc acctacgctc gctgcggcaa
     4621 gcgccccgcc cggccgggcg cacccaggta cttgcggccg aggtagacgc ccgccactgc
     4681 cgccacgccc aacggtgcca gccacgcgag gttctggaac accggtccct gcagcatcgg
     4741 cagcaggcgc ggcaggccga tcgacaggaa cgcgatgtgg gtggcgacgc cgttgccgag
     4801 catcgcgcgc aggtgctggc gcatccacca gcgcgcttcg gtcggcggct gcagggcgaa
     4861 ccggcgcatg ctggcgaacg tgctgatgcc gatcagcgag aagctgacga tgatcagctg
     4921 catccggtcc gccacgaaca ggccgaacag tgcaatgccg gcaccggcgc cgaggttgag
     4981 ccacatcagc gcgcggtagg cggggccgac atagcgcgcc cagtcgcgcc tgtcgcggat
     5041 cgcgcgccag ctcagccagg tgctggtgca ggtgatcacc agcaggtaca gcaggaacac
     5101 cacgatcttc tggtggccgg cgaaccacgc cagccagcac atcggcacgg tggccgcgag
     5161 caggcccgcc atcgccagca ggtacacctt gccggcgccg acatgcagcg gaccgccctt
     5221 gcgcgcgatg ccggcggtcc agaacgccac cagcgccacg cagcccagcg cgatgtggac
     5281 cagcttgaag gcaaggtacg gattcatggc aatgctcgcg acgggaggtg gccagtctcc
     5341 ggcgccgcga ccgccacctc aggtgccgcg ggtcatcggc gcgaggtgcc ggaagtcatt
     5401 ttctgcgccc gccgcattgt ttgcggcctg atctccgcgc agcatgtcca gatgcttgcc
     5461 ttcctgcgca ccgccttcgc tcctctgaac ctggccgcgc tactggcgat cggcggcatc
     5521 ggcatgtcgt tgcgctggtt gccgggcgaa atgcgcagcg gggcgttcac cctgctgatc
     5581 gcctacacct gctgcttgtt gctggcgacg tttccgcagc ggcggcgctg ggcacagaat
     5641 gcggcgttat tgctgatgcc gttgctggcg ctggcggcca tcgcggtcgc gccgcgaatg
     5701 ggcacggcac agatcctgct ggtcgtgtgg gcggcagtcg tggtgatggc gtggccgccg
     5761 ctggtggtcc tggcggcaat tgtcgcggta gacctcgccg catttggggt cctgcgtcat
     5821 gctggccatt ccaacccggt gatcaccacc attctctacg ccagcttcca gggcttcgcc
     5881 gcattgactt cgcactacgc acgcagcgcc gaggaagcgc gcgaccggct ggcacgaacc
     5941 aatgcggacc tgctggcgac gcgcgccctg ctggccgacg ccgcgcgcga cggcgaacgc
     6001 ctgcgggtcg cgcgcgaact gcacgacgtc gccggccaca agctcaccgc gctgcgcctg
     6061 aacctgcgcg cgctgcaggc ggccgcaccg tcgccgcaac tgcagctggc cgagcaactt
     6121 tccgccgaac tgctgggcga catccgcggg gtggtgcatg cgctgcgcga tgccggagga
     6181 ctcgacatcg acaccgccct gcgcgcattg gccgcgccct acccgcggcc ggcgctgcgg
     6241 ctgtccatcg atgaaggcgt ggtggtcgag gatccggccc tcgccgaaac cgtgctgcgc
     6301 accgtgcagg aagcgctgac caatgccgcc cgccatggcc acgccgctac cctcgacgtg
     6361 cgcctgcgcc gcgatggcac ctggctgtgg gtggagatcg aggacgacgg ccgcgccagc
     6421 ctgccgctgc gcgagggcca cggcctgacc ggcatgcgcg agcgcatcga cgcgcgcggc
     6481 ggccgcctgc ggatcgcgcg cggatcgcgc ggcggcgtgc gcatcgaagc gggcgtgccg
     6541 gcatgagcgc gacaccggtt cgggtcgccc tcgccgacga ccaggccctg gtccgcgccg
     6601 gcctgcgcgc gctgctgcaa cagcagggca tcgacatcgt gttcgaggcc gacgacgggc
     6661 aagccctgct ggatgcgctg cacacggcgc cggtcgacat catcctcagc gacatccgca
     6721 tgcccgggct ggacggaatc gccgcgctgg aggccctgcg cgcgcgcggc gacgccaccc
     6781 cggtgctgct gctgaccacg ttcgacgatg ccgaattgtt gctgcgcgcg accgaagccg
     6841 gcgcccaggg cttcctgctc aaggacgcgg cccccgacga cctgcgcgag gcgatcctcc
     6901 gcgtcgcccg cggcgaaacc ctgctgcagc cggtcagcac cgacgcggtg cgcgcgcgct
     6961 accgctacca cgccgacgac gcgcccccgc gcgacacctt cggcaagcgc gaggtcgcga
     7021 tcctgcgcct gatcgccggc ggctattcga accgggaaat cgcgcgcagc ctgttccttg
     7081 ccgaaggcac ggtgaagaac tacgtgtccg agatcctcga caagctgggc acccgcgacc
     7141 gcacccgcgc ggtgctgaag gcgatcaccc tgcggatcat ctgaaatgcc gccgccctcg
     7201 ccgcgccatg cagtcccgct atgcttggcc gggacgatcg gaagggcatg aagatggcga
     7261 gattcgacgc atggtggcgg tgggcgcggc aatggcgcca ggcgcggctg gtcgacacca
     7321 tcgaccgcgt cgcggtgctg gaaaacgtcc acgacggtgg cgcgctgggc ccgcgttatg
     7381 ccttcatgat cgtgatggcc tgcggcatcg ccatgctcgg cctgctgcag aattcggtgg
     7441 cggtgatcat cggcgcgatg ctgatctcgc cgctgatggg gccgatcgtc cagctcggca
     7501 tgggcctggc cacgttcgag ctgcgcggca tccgcgactc gctgcggacg ctgctggcgg
     7561 gggtcgcgct ggcactggcg atcgcgatcc tgatcgtctg gatgtcgccg ctgcaggcca
     7621 gcaccagcga gatcctggca cgcaccgagc cgaccttctt cgacctgctg gtggcggtgt
     7681 tctccggcct ggcgggggcc tacgccacgg tcacgcgcaa gggcgaaacc atcgtcgggg
     7741 tggcgatcgc caccgcgctg atgccgccac tggcggtggt cggctacggc atcgccctcg
     7801 gcaactggac gatcgccggc ggcgccgcat tcctgttcat gaccaacctg ctggcgatcg
     7861 cgctgtcggt gaccatcgtc gcccggttgt acggcttcgg cggctccgac agccccaagc
     7921 agactgcctg gcaggccggc ctgatcgtgg ccagcttcgt gctgctgtcg gtgccgctgg
     7981 gactggcgct caagcgcatc gccctgcagt cgcagaccga attgctggtg cgttccgcac
     8041 tggaggagag cgccgcgcgc gcatccggcc gggtgtcggc gctgcgggtg gagtccggtg
     8101 ccgacggcgt cgcggtggag gcggtggtgc tgatgccgga tcacgtgccg ggcatggagg
     8161 cgaccctgca gacccagctc gctgcgcggc tcggacgccc ggtcagcgtg cgcgtgcagg
     8221 aagtgctgac cgccgacgac gccagcttcg gccgccagca aggggcattg gccgaactgc
     8281 ggcgcagcgt gagcgcgctg caggatgccg aaagcgtgcg cagcaaccag cagcgcgcgc
     8341 gcgccgagga acgtgccgcg ctgcaggcat cgctgctggc ctacctgggc cggctgcagc
     8401 gcagtgcgga tggcagccgc tgggaactgt gggtggcggc ggagacgcgc atgccgctgg
     8461 cgcgggcatt ccggatcgaa gccgagctcg aagccgcccg cgccgacggg gcgccggtgc
     8521 tgcgggtgtt cccgccgctg caggcgctgc cggcgatcgc gctggaaacc accgaggccg
     8581 acggcccggg ccccggcgaa gccgcgctcc gttccctcgc catccaggcc tgggcgctgc
     8641 aacgctggcg cgcgggcacg gtggacgcgc gcctggccag cgacgacgac gcccaggccg
     8701 aggcgtggac cgcggccctg cgggaggcac tgtcggcgcg cgggatcacg cttggccgcg
     8761 tcgagcgcag cagcgaaggc gcgcccggcc tgcagctggc actgcctgcg ccgtagccct
     8821 ggtcagggtt tcgccggcca gccggcgggg acctcgaggt agcctcccga caggtcgatg
     8881 ccatccacgt ccgcaccggg cgcgaccgcc acttcgatca actcgtccgg cgggcagggc
     8941 gcgcggatgc agcggatctg cgaggcatgc gcaagcagcg ccagttcacc cgcctgcacc
     9001 cagcccatca ggtaatagcg gccgggggca acgcccaggc ggtagtcgcc ggagccggcc
     9061 ggcgagtcgg tgcaggtggg cgcgccgccg ccgaccggat gcgcgcagat gcgcagggcc
     9121 ggcggcgggc ggttgccgtc gcggatcgcg ccgcggatgc tggcgccggt agccgtcgcc
     9181 ggcgttgccg ccgggtcggc agccgcggcg ggaggcgcat gcgcgggtgc ggctgccggc
     9241 ggcggggcgg gactggccgc cggtgcggac gcgccgatcg ccggggtggt ggtggcgggc
     9301 gcgcaggcgc tgagcggcag cgcggcgaac agcaggatcg gcagcatccg cggcatggga
     9361 cctcctccgg gtggcgggct tcggcgacaa tagcgcctcc cgccgccgcc tgcccgccga
     9421 tgacgccgac cccgatttcc ctcacccgct tcctgatcga ggaacaacgc gccggccgca
     9481 tcagccccga cctgcggctg ctggtcgaag tggtggcgcg cgcctgcaag agcatctcga
     9541 tcgcggtcgg caagggcgcg ctgggcggcg tgctcggcga tgcgctcagc gccggcgagg
     9601 ccagcatcaa cgtgcagggc gaggcgcaga agaagctgga cgtgctcagc aacgaaatcc
     9661 tgctggaggc caacgcctgg ggcggccacc tggccgggct ggcgtcggag gagatggacc
     9721 acagcatgcc gatcccggac gcctacccgc gcggcaatta cctgctgctg ttcgatccgc
     9781 tggacggcag ttcgaacatc gacgtcaaca tctcggtcgg caccatcttc tcggtgctgc
     9841 gctgcccgga cggggtcacc catcccggcg acgaacactt cctgcagccc ggcaccgccc
     9901 aggtcgcggc cggctactgc acctacggcc cgagcacgat gctggtgctg accgtgggcc
     9961 acggcaccca cgcgttcacc ctcgaccgcg aggtcggcag cttcgtgctg accacccgcg
    10021 gcatgcggat cccggaggag acccgggaat tcgcggtcaa catgtccaac cagcgcttct
    10081 gggaagcgcc gatgcaggcg tacgtcggcg acctgctggc cggcagcgaa ggcccgcgcg
    10141 gcaaggactt caacatgcgc tgggtggcga gcatggtcgc cgacg
//
LOCUS       DAORUK010000069        10085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_7064_length_10085_cov_18.331805, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000069 DAORUK010000000
VERSION     DAORUK010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10085
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7064_length_10085_cov_18.331805"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="PK818_06135"
     CDS             <1..1311
                     /locus_tag="PK818_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQY82347.1"
                     /translation="LPSLKGARWIAIGRLDINTTGLLLLTTDGELANAMMHPSFEVAR
                     EYVCRVRAPEGEDVVSEKIIDRLARGVALDDGPAKFDEIERIGGTDSHDWFRVLLKEG
                     RNREVRRLWESQGCQVSRLKRIRYGDVRLPQPLLRGQSQELPQAEVDGLRKAVGLEDG
                     PPSALTLMPVLGQRKASKATVHVGGHARAPGYVGGHSTADEGRELRRFDNVREDRGRG
                     RGGPRKPGGLTVSGEAAAKQANKAFKPKGPRPQGQRLRPGEQRGENPAEMRTWYVPDG
                     VDTGPSGHRNPDGPGGPRKPYGNKPGGSRPGAPRGPGAPRPYGENRGPRPAGARGPGG
                     PRREGGPGAGDRGPRREGGPGHGEGRPARPARPYGHPGNAPSFPSDHANPGGFNPYAE
                     RPRSTRPAGPRPAGNRPGPGGPRGPRPGGSRGPRGGGGHQGGNR"
     gene            1514..2386
                     /locus_tag="PK818_06140"
     CDS             1514..2386
                     /locus_tag="PK818_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HQY82348.1"
                     /translation="MKSILNVTSAPDRHWVGDGFPVHGMFGYSGPGVPQRSPFLMLDY
                     AAPTRFEPTARRRGVGQHPHRGFETVTIVYDGEVEHRDSTGQGGVIGPGDVQWMTAGS
                     GILHEEFHSADYGKRGGPFEMVQLWVNLPKQDKLTAPGYQAILDAQIPAVALPDGAGT
                     VRIIAGAYAGAAGPARTFSPMNVLDVRLAAGASAALPQPEGWTTLLVVLAGSVRLGGE
                     SVLGEKQMATLSTTGTGVVIEASGDAKLLLLAGEPIDEPVVGYGPFVMNSPQEIREAI
                     GDFNSGKFGQMPAR"
     gene            2502..4514
                     /locus_tag="PK818_06145"
     CDS             2502..4514
                     /locus_tag="PK818_06145"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQY82349.1"
                     /translation="MDVGTLLRRGLAVVLLCVALPVVAATANPSEPAAIAALRADQSR
                     TAAQLEAEASRLRAGSQDPKFRAWATLAEAEAANDQEHAEAALAGLDQAIAQADALKL
                     PDLRFEALIRLSTILVNRGRSKETEAVLKQMQAMVEAGDNPRWRAQWLHERGVLERKQ
                     GRFEEARSLFLQARSLFHATGDAVAEARELNSIGNLDGRTGRFSDAVAMHGDALAMAR
                     KAGDKAETARSLRMLGVLYRNLDDEELASRYLLEALSYVEERNRREAIALHGELIGAF
                     TRLEKLPEAEHHGQQAVRLAELSGSHPNKVNAFTRVAELRLLQGRIDEAEQWVERANA
                     SYDHVAVRDKTLVRISRLRVLAARNNTAQALKEADVVLADVRDLGDRILERSLLDLLS
                     ELQLRAGDAASAFATRKAHQQLDKELAIDVAARRIALLESSLERERTEAERQLLQRDN
                     DIQALRLNRQRVIAIALLAGLALVLAGAALLYARYRSALRLRNELRASRDALERVANT
                     DALTGLANRYAATRALGERLGRVPEPLTVMLLDLDEFKRINDSHGHQAGDAVLREVAT
                     RMRAVLPPGALLGRWGGEEFIAILEGAAGREPGAIAEALRAAVAAAPVPFESKAIPIT
                     VSIGVATAFSTDDMDALLAEADEALYRAKNSGRNRVVYGGSPVPAG"
     gene            complement(4519..5325)
                     /locus_tag="PK818_06150"
     CDS             complement(4519..5325)
                     /locus_tag="PK818_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017160359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQY82350.1"
                     /translation="MNNLRVSIVQGETRWHDPAGNRSHYGELIAPLHGTTDLVVLPET
                     FTSGFSNDAIGDAETMHGPTVAWMREQAVKLDAAVTGSVQLRTDAGVFNRMLFATPDG
                     GLLHYDKRHLFSFAREHERYAAGHARVIVEWKGWNILLQVCYDLRFPVFSRNRFDVAR
                     ERLPDYDLALYVANWPAARSYPWKTLLRARAIENLCYVVGVNRVGSDGNGLQYSGDSA
                     VIDFLGHPLSEATDQEVVSTMLLQAAPLVEHRARFPALLDGDAFQLGTGG"
     gene            complement(5322..6467)
                     /locus_tag="PK818_06155"
     CDS             complement(5322..6467)
                     /locus_tag="PK818_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007964632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQY82351.1"
                     /translation="MPASKLPKVGTTIFTVMSQLAVEHGAVNLGQGFPDFPVPERLVD
                     ALGRAMRAGHNQYPMMTGIPALRQAIAAKTWRCHGWRPDPDSEVTVTSGATEAIFDAI
                     HAVVRAGEEVIVLDPCYDCYEPAIELAGARAVHVALDPASFAPDWAAVRAAITPATRM
                     LMINSPHNPSGAMLGDADLRELIAILDGTDILLLSDEVYEHIVFDGARHESVLRHPQL
                     RERAFVVSSFGKTYHCTGWKLGYCIAPPALTAEFRKVHQYNTFCSFNPAQHAFAEMIE
                     QEPEHYEGLGAFYQAKRDRFRERLLATRLKPLAVPGGYFQLVDYSAVSDLGDVDFCRW
                     LTIDKGVTAIPLSPFYETPPAGQRLVRLCFAKNEATMDAAIERLQAL"
     gene            6574..7218
                     /gene="ccmA"
                     /locus_tag="PK818_06160"
     CDS             6574..7218
                     /gene="ccmA"
                     /locus_tag="PK818_06160"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HQY82352.1"
                     /translation="MPDHDVSPSPLLAVRGLAFSRDETPVFGPLDFSVDANEALLVQG
                     GNGAGKTTLLRVLAGLLRADAGVVEIEGLPARAESRARHIAYLGHLPGLKADLGALQN
                     LEFLCGLHGRRKAQAPEAAMGIVGLGGYEDAAARTLSAGQKKRLGLARLWLSPAPLWL
                     LDEPYANLDLDGINLVNRMVQAHLRSGGAALITTHGAYAAPPVRTRMLQLGAAA"
     gene            7215..7886
                     /gene="ccmB"
                     /locus_tag="PK818_06165"
     CDS             7215..7886
                     /gene="ccmB"
                     /locus_tag="PK818_06165"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HQY82353.1"
                     /translation="MMRQAARALLRRDLQLLWVRRGDALQPALFALLTVVLFALGLGG
                     EAESLAPVASAVLWLAVMLAGLLALDSLFRGDAEDGSLEQWMLAPVPLWWLVLVRTLV
                     HWLTTALPVIVAVPLLGELLHLPRAQLPVLMASLLLGTPVLSLLGAVVAALTVGMRRA
                     GVLVALLALPLYVPVLVFGAGAVAASAQGLDAVGALLLLAAGLVIALVLAPLAAAAAL
                     RIAHN"
     gene            7891..8655
                     /locus_tag="PK818_06170"
     CDS             7891..8655
                     /locus_tag="PK818_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HQY82354.1"
                     /translation="MNLSAKNRLVRWFHQLGSPPYFDRFATRWAPWAYLLGLATMAVG
                     LYGALFQVPADYQQGDSFRILYIHVPAAWMSMAIFAAMAFYSAIALVWRIKLCEILAM
                     ACAPTGAAFTLVTLATGSIWGKPMWGTWWDWDPRLTTELILLFLYLGVMGLYAAIDDR
                     RNAARAAGFLAIVGVALLPVIRWSVVWWNSLHQGQTIRLLGESSMDASMLAPLAWMII
                     GTKLWFLGSLLSRARADNLRREAGKDWVARLSGGTP"
     gene            8652..8816
                     /gene="ccmD"
                     /locus_tag="PK818_06175"
     CDS             8652..8816
                     /gene="ccmD"
                     /locus_tag="PK818_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012438189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HQY82355.1"
                     /translation="MSYREYVIAAYAVFAIVLLWDYVVPKLQVRAALRAARLRASRTA
                     TRNPPTELQR"
     gene            8813..9277
                     /gene="ccmE"
                     /locus_tag="PK818_06180"
     CDS             8813..9277
                     /gene="ccmE"
                     /locus_tag="PK818_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014090328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HQY82356.1"
                     /translation="MNPTRKRRLWLVLAVVAAAAIATTLIAMALQRNVAYLYTPSEVL
                     RGEAGQHARFRLGGMVAAGSFKRAQGSMEAHFAVDDGDATLPVVYTGILPDLFREKQA
                     VVATGRMQGDVFVAEEVLAKHDETYMPKEVADKMGAAHKKHDVQAPAGAGAN"
     gene            9278..>10085
                     /gene="ccsA"
                     /locus_tag="PK818_06185"
     CDS             9278..>10085
                     /gene="ccsA"
                     /locus_tag="PK818_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQY82357.1"
                     /translation="MLAELGQVALVLALMVALLQALLPLLGAQRGIAPLMAIARPAAF
                     LQFALVALAFAILTHGFVIHDFSLRYVAENSNSLLPMVYRYTAVWGAHEGSLLLWALI
                     LAAWTAAVAGFSRSLPMPVVARVLGVMGVVAVGFLAFLIFTSNPFERLLPAAADGRDL
                     NPLLQDPGMIIHPPMLYFGYVGFAVPFAFAIAALLEGRVDAQWLRWTRPWTNVAWGFL
                     TLGIAIGSWWAYYELGWGGWWFWDPVENASFMPWLAGAALLHSQAVTEKRG"
BASE COUNT         1447 a         3423 c         3656 g         1559 t
ORIGIN      
        1 ctgccctcgc tgaagggcgc gcgctggatc gcgatcggcc gcctcgacat caacaccacc
       61 ggcctgctgc tgctcaccac cgatggcgag ctggccaacg cgatgatgca tccgagcttc
      121 gaggtcgccc gcgaatacgt ctgccgcgtg cgcgcgccgg aaggcgagga cgtggtcagc
      181 gagaagatca tcgaccgcct cgcccgcggc gtggcgctgg acgacggccc ggccaagttc
      241 gacgagatcg agcgcatcgg cggcaccgat tcccatgact ggttccgcgt gctgctgaag
      301 gagggccgca accgcgaggt gcgccgcctg tgggaatcgc agggttgcca ggtcagccgg
      361 ctgaagcgga tccgttacgg cgacgtgcgc ctgccgcagc cgctgctgcg cgggcagtcg
      421 caggagcttc cgcaggccga ggtggatgga ctgcgcaagg ccgtggggct ggaggacggc
      481 ccgccgtccg cgttgaccct gatgccggtg ctgggccagc gcaaggcgag caaggccacc
      541 gtgcacgtcg gcggccatgc gcgcgcgccg gggtacgttg gcggccacag caccgccgac
      601 gaagggcgcg agttgcgccg cttcgacaac gtgcgtgaag accgcggtcg tgggcgcggt
      661 ggtccgcgca agcccggcgg cctgaccgtg agcggcgagg cagccgcgaa gcaggcaaac
      721 aaggcgttca agccgaaggg gccgcgcccg cagggccagc gcctgcgccc gggtgaacag
      781 cgcggcgaga atccggccga gatgcggacc tggtacgtgc cggacggtgt cgacaccgga
      841 cccagcgggc atcgcaaccc ggatggcccg ggcggcccgc gcaagcccta tggcaacaag
      901 cctggcggaa gccggcccgg tgcgccgcgc ggccccgggg caccgcgccc ctatggcgag
      961 aatcgcggtc cacgcccggc gggtgcccgc gggcccggcg gtccacgccg tgaaggtggc
     1021 cccggcgccg gggatcgtgg cccacgtcgc gaaggcggcc ccggccatgg cgaaggccgt
     1081 cccgcgcgcc cggcccggcc gtatggccat cccggcaatg cgcccagctt cccgtcggac
     1141 cacgccaacc cgggcggatt caatccgtac gcggagcgtc cgcgcagcac gcgacccgcg
     1201 ggtccgcgcc cggccggcaa ccggccgggt cccggcggtc cgcgcggccc caggcctggc
     1261 ggcagtcgcg gtccgcgcgg cggcggtggg caccagggcg gcaatcgctg atcgaaggtt
     1321 gcaggggtgc ccaagggcac ccttcgcatt tgcgcgatgg cgtccggcgt ggccggatga
     1381 ttatcgcacc agtggaacaa ttcgatacat tgcaaccagc tagtatcact ggtggtcgta
     1441 atctaccgtg cctcgccatg acatccggca ctccgccggt tcgcatcgga gccacgccat
     1501 gacgaccatg cgcatgaaaa gcatcctcaa cgtcaccagc gcccccgacc ggcattgggt
     1561 cggcgacggc ttcccggtgc atggcatgtt cggttacagc ggccccggcg tgccccagcg
     1621 cagcccgttc ctgatgctgg attacgcggc cccgacccgc ttcgaaccca ccgcccgccg
     1681 gcgtggcgtg ggccagcacc cgcatcgcgg tttcgagacc gtcaccatcg tctacgacgg
     1741 cgaggtcgag caccgcgatt ccaccggcca gggtggcgtg atcggcccgg gcgacgtgca
     1801 gtggatgacc gccggcagcg gcatcctcca cgaggaattc cattcggccg actacggcaa
     1861 gcgcggcggc ccgttcgaga tggtgcagct gtgggtcaac ctgccgaagc aggacaagct
     1921 gactgccccg ggctaccagg ccatcctcga cgcacagatc cccgcggttg ccctgcccga
     1981 tggcgccggc acggtgcgca ttatcgcggg cgcctacgcg ggcgccgccg gtcccgcgcg
     2041 caccttcagc ccgatgaacg tgttggacgt gcggctggcc gccggtgcat cagccgcatt
     2101 gccgcaaccg gagggctgga ccaccttgct ggtggtgctg gcgggttcgg tgaggcttgg
     2161 cggcgaatcg gtgttgggcg aaaagcagat ggcgacgctg tccaccaccg gcaccggcgt
     2221 ggtcatcgag gccagtggtg atgcgaagct gctgcttctc gccggtgaac cgatcgacga
     2281 gccggtggtc ggctacgggc cgttcgtgat gaacagcccg caggaaatcc gcgaggccat
     2341 cggcgatttc aacagcggca agttcgggca gatgccggcg cgctgacaac ccggccaccg
     2401 gcgcaaaccc gcgaccggcg gcgcttcgcg caaccgggcg gatctggcat gctggcggcg
     2461 gatcttgggg accgcagatg ccagggtatg cgcaccggta cgtggatgtt ggcaccctgc
     2521 tgaggcgtgg cctcgcggtc gtgctgctgt gcgtcgccct gccggttgtc gccgcgaccg
     2581 ccaaccccag cgaaccggcc gcgatcgccg cgctgcgcgc cgaccagtcg cggaccgccg
     2641 cccagctcga agccgaggca tcgcgcctgc gcgccgggtc gcaggacccg aagttccggg
     2701 cctgggcaac ccttgccgaa gccgaggccg cgaacgacca ggaacatgcc gaagccgcgc
     2761 tggcgggact ggaccaggcg attgcacagg cggacgcgct gaagctgccc gacctgcgct
     2821 tcgaggcgct gatccggctg tccacgatcc tggtcaaccg cggtcgctcg aaggagacgg
     2881 aagcggtcct caagcagatg caggcgatgg tcgaggccgg cgacaacccg cgttggcgcg
     2941 cgcaatggct gcacgaacgc ggcgtgctgg agcgcaagca gggccgcttc gaggaggccc
     3001 gcagcctgtt cctgcaggcg cgctcgctgt tccacgccac cggcgacgcg gtggcggagg
     3061 cgcgcgaact gaattccatc ggcaacctcg acgggcgcac cggtcgcttc tcggatgcgg
     3121 ttgcgatgca tggcgatgcg ctggcgatgg cacgcaaggc cggcgacaag gccgagaccg
     3181 cgcgcagcct gcgcatgctc ggcgtgctct atcgcaacct ggacgacgag gaactggcga
     3241 gccgttacct gctggaggcc ctgagctacg tcgaggaacg caaccggcgc gaggcgatcg
     3301 ccctgcatgg cgagctgatc ggtgcgttca cccggctcga gaaactgccg gaggccgagc
     3361 accacggcca gcaggcggtg cgcctggccg agctttccgg gagccacccc aacaaggtca
     3421 acgccttcac ccgggtcgcg gagctgcgcc tgctgcaggg gcgcatcgac gaggcggaac
     3481 agtgggtcga gagggcgaat gcctcctacg accacgtcgc cgtgcgcgac aagaccctgg
     3541 tccgcatcag ccgcctgcgc gtgctcgccg cgcgcaacaa caccgcgcag gcgctgaagg
     3601 aagccgacgt ggtgctggcg gacgtgcgcg acctcggcga ccgcatcctg gagcgttccc
     3661 tgctcgacct gctgtccgaa ctgcagttgc gcgccggcga tgccgccagc gccttcgcca
     3721 cccgcaaggc gcaccagcag ctggacaagg agctggcgat cgacgtcgcg gcgcgccgga
     3781 tcgcgctgct ggagtccagc ctggagcgcg agcgcaccga ggcggagcgc cagcttctgc
     3841 agcgcgacaa cgacatccag gccctgcgcc tgaaccggca gcgggtgatc gcgatcgcgc
     3901 tgctggccgg cctggcgctg gtgctggcgg gagcggcatt gctgtatgcg cgctaccgca
     3961 gcgcgctgcg gctgcgcaac gagctgcgcg ccagccgcga tgcgctggag cgggtggcca
     4021 acaccgatgc cctgaccggg cttgcgaacc gctacgcggc gacgcgtgcg ctgggcgagc
     4081 ggctcggccg ggtcccggaa ccactgacgg tcatgctgct cgacctcgac gaattcaagc
     4141 ggatcaacga cagccatggc caccaggccg gcgacgcggt gttgcgcgaa gtggccacgc
     4201 ggatgcgcgc agtgctgccg ccgggcgcgc tgctgggtcg atggggtggc gaggaattca
     4261 tcgccatcct cgagggcgcc gccgggcgcg agccgggggc gatcgcggag gcgctgcgtg
     4321 cggcggtcgc ggccgcgccg gtgccgttcg agtcgaaggc gatcccgatc acggtcagca
     4381 tcggggtcgc aaccgcattt tccaccgacg acatggatgc gctgctcgcc gaagccgacg
     4441 aggcgctgta tcgcgccaag aacagcggcc gcaaccgggt ggtctacggc ggttcgccgg
     4501 tgccggcggg ctgaaccctc agccgccggt accgagctgg aaggcgtcgc catccagcag
     4561 cgccgggaag cgtgcgcgat gctccaccag cggcgcggcc tgcaagagca tggtcgacac
     4621 aacctcctgg tcggtcgcct cgctcaacgg gtggccaagg aagtcgatga ccgcgctgtc
     4681 gccgctgtac tgcaggccgt tgccatcgct gccgacccgg ttgacgccaa ccacgtagca
     4741 gaggttctcg atcgcgcgtg cgcgcagcag ggtcttccac ggatagctgc gcgccgccgg
     4801 ccagttggcc acgtacagcg ccaggtcgta gtccggcagc cgctcgcgtg ccacgtcgaa
     4861 acggttgcgc gagaacaccg ggaagcgcag gtcgtagcag acctgcagca ggatgttcca
     4921 gcccttccat tcgacgatca cgcgcgcatg cccggccgcg tagcgctcgt gctcgcgggc
     4981 gaaggaaaac aggtggcgct tgtcgtaatg cagcagcccg ccgtcgggcg ttgcgaacag
     5041 catgcggttg aacacgcctg cgtcggtacg cagttgcacg ctgccggtga ccgcggcatc
     5101 gagtttcacg gcctgctcgc gcatccaggc cacggtcggg ccatgcatgg tctcggcatc
     5161 gccgatggca tcgttggaga acccgctggt gaaggtctcc ggcagcacca caaggtcggt
     5221 ggtgccgtgc agcggcgcga tcagctcgcc gtaatgggaa cggttgccgg caggatcgtg
     5281 ccagcgggtt tcgccctgga cgatggaaac gcggaggttg ttcatagcgc ctgcaagcgt
     5341 tcgatcgcgg cgtccatggt cgcctcgttc ttggcgaagc acagccgcac caggcgctgc
     5401 ccggccggcg gggtttcgta gaacggcgac agcgggatcg cggtgacgcc cttgtcgatg
     5461 gtcagccagc ggcagaagtc gacgtcgccg aggtcgctga ccgcgctgta gtcgaccagt
     5521 tggaagtagc cgcccggcac cgccagcggc ttcagcctgg ttgccagcag tcgttcgcgg
     5581 aagcggtcgc gcttggcctg gtagaaggcg ccgagcccct cgtagtgctc cggctcctgc
     5641 tcgatcatct cggcgaaggc gtgctgggcc gggttgaagc tgcagaaggt gttgtactgg
     5701 tggaccttgc ggaattccgc ggtcagcgcc ggcggcgcga tgcagtagcc gagcttccag
     5761 ccggtgcagt ggtaggtctt gccgaagctg gacaccacga acgcgcgttc gcgcagttgc
     5821 ggatggcgca gcaccgattc gtggcgcgcg ccatcgaaca cgatgtgctc gtagacctcg
     5881 tcgctgagca ggaggatgtc ggtgccgtcg aggatcgcga tcagttcgcg caggtcggcg
     5941 tcgcccagca tcgcccccga ggggttgtgc gggctgttga tcatcagcat ccgggtggcc
     6001 ggggtgatcg cggcgcgcac cgcggcccag tccggggcga agctcgccgg gtccagcgcc
     6061 acgtgcaccg ccctcgcccc cgccagttcg atcgcgggtt cgtagcagtc gtagcagggg
     6121 tcgagcacga tcacttcctc gccggcgcgc accaccgcgt ggatggcgtc gaagatcgcc
     6181 tcggtggcgc cgctggtgac ggtgacttcg ctgtcggggt cgggccgcca gccatgacag
     6241 cgccaggtct tggcggcgat cgcctgccgc agcgccggga tcccggtcat catcggatac
     6301 tggttgtggc cggcgcgcat cgcccgcccg agggcgtcga ccagccgttc cggcaccggg
     6361 aagtcgggga agccctggcc caggttgacc gcgccgtgct ccacggcgag ctgcgacatc
     6421 acggtgaaga tggtggtgcc gaccttcggc agcttgctgg cgggcatggg tggggatgtg
     6481 ggcatcgagg cgttgtccgt tgcgatccgg cgagtttacg aaatccgcgc cacccggtcc
     6541 gcgcactggc catgccggta gaatcgaccc ggcatgcctg accacgacgt ctcccccagc
     6601 ccattgctcg ccgtgcgcgg gctggcgttt tcccgcgacg agacgccggt cttcggtccg
     6661 ctggatttct cggtggacgc caacgaggcg ctgctggtgc agggcggcaa tggtgccggc
     6721 aagaccaccc tgctgcgggt gctggccggc ctgttgcgcg ccgatgccgg tgtggtcgag
     6781 atcgagggcc ttcctgcgcg cgccgaatcg cgcgcgcgcc acatcgccta cctgggccac
     6841 ctgcccgggc tgaaggccga tctcggcgcg ctgcagaacc tggagttcct gtgcggcctg
     6901 catggccgtc gcaaggcgca ggcgccggag gcggcgatgg gcatcgtcgg cttgggtggt
     6961 tacgaggacg cggccgcgcg caccctttcg gcggggcaga aaaagcggct cgggctggcc
     7021 cgcctgtggc tgtcgccggc cccgttgtgg ctgctcgacg agccctacgc aaacctcgac
     7081 ctggatggca tcaacctggt caaccgcatg gtgcaggcgc acctgcgcag cggcggcgcg
     7141 gccctgatca ccacccatgg cgcctacgcg gcaccaccgg tgcggacgcg gatgctccag
     7201 ctcggggccg ccgcatgatg cgccaggccg cccgcgccct gctccggcgc gacctgcagt
     7261 tgctgtgggt gcgccgcggc gatgcgctgc aaccggccct gttcgccctg ttgacggtgg
     7321 tgctgttcgc gcttgggctg ggtggcgaag cggaatcgct ggcgccggtg gcgtccgcgg
     7381 tgttgtggct ggcggtgatg ttggccggcc tgctggcgct ggacagcctg ttccgcggcg
     7441 acgccgagga cggctcgctg gagcaatgga tgctggcgcc ggtgccgctt tggtggctgg
     7501 tgctggtgcg caccctggtg cactggctga ccaccgcgtt gccggtgatc gtggccgtcc
     7561 cgctgctcgg cgaactgctg cacctgccac gcgcgcaact gccggtgctg atggccagcc
     7621 tgctgctggg cacgccggtc ctgagcctgc tcggcgcggt ggtggccgca ctcacggtgg
     7681 gcatgcgccg cgccggggtg ctggtcgccc tgctggcgct gccgctgtac gtgccggtgc
     7741 tggtgttcgg cgcgggcgcg gtggcagcct cggcgcaggg cctggatgcg gtgggggccc
     7801 tgttgctgct cgccgccggg ctggtgattg cgctggtgct ggcgccattg gcagctgcgg
     7861 cggcgctgcg catcgcccat aattgaccca atgaacttat ccgccaagaa ccggctggtc
     7921 cgctggttcc accaactcgg ttcgccaccc tacttcgatc gcttcgccac gcgctgggcg
     7981 ccatgggcgt acctgcttgg cctggcgacg atggccgtcg gcctgtacgg cgcgctgttc
     8041 caggtcccgg ccgactacca gcagggcgac agcttccgca tcctctacat ccacgtcccg
     8101 gcggcgtgga tgagcatggc gatcttcgcc gcgatggcgt tctactcggc catcgcgctg
     8161 gtctggcgga tcaagctgtg cgagatcctg gcgatggcct gcgcgcccac cggcgccgcc
     8221 ttcaccctgg tcaccctcgc caccggttcg atatggggca agccgatgtg gggcacctgg
     8281 tgggactggg acccgcgcct gaccaccgag ctgatcctgc tgttcctgta cctgggcgtg
     8341 atggggctgt atgcggcgat cgacgaccgc cgcaacgccg cccgcgcggc cgggttcctg
     8401 gccatcgtcg gggtcgccct gctgccggtg atccgctggt cggtggtgtg gtggaactcg
     8461 ctgcaccagg gccagaccat ccgcctgctc ggcgaatcct cgatggatgc cagcatgctc
     8521 gcgccactgg cgtggatgat catcggcacc aagctgtggt tcctcggttc cctgctcagc
     8581 cgcgcccgcg ccgacaacct gcgccgcgag gccggcaagg attgggtggc caggctgtcc
     8641 gggggcacgc catgagctac cgcgaatacg tgatcgccgc ctacgcggtg ttcgccatcg
     8701 tcctgctgtg ggactacgtg gtgccgaagc tgcaggtccg cgccgccctg cgcgccgcgc
     8761 gcctgcgcgc atcgcgcacc gccacccgca acccgccaac ggagctgcaa cgatgaaccc
     8821 gacccgcaag cgccgcctgt ggctggtgct ggccgtggtg gccgcggccg cgatcgcgac
     8881 caccctgatc gcgatggcgt tgcaacgcaa tgtcgcctac ctgtacaccc cgagcgaagt
     8941 gctgcgcggc gaggcaggcc agcacgcccg cttccgcctc ggcgggatgg tcgcggccgg
     9001 ctcgttcaag cgcgcgcaag gctcgatgga agcgcacttc gcggtggacg acggcgatgc
     9061 caccctgccg gtcgtctaca ccgggatcct gcccgacctg ttccgcgaaa agcaggcggt
     9121 ggtcgccacc ggacgcatgc agggcgatgt gttcgtcgcc gaggaagtcc tggccaagca
     9181 cgacgagacc tacatgccga aggaagtcgc cgacaagatg ggcgccgcgc acaagaagca
     9241 cgacgtgcag gcgccagccg gcgcgggcgc caattaggtg ttggccgaac tcggccaggt
     9301 agcgctggtg ctggcgctga tggtggcgct gctgcaggcg ctgctgccgc tgctgggcgc
     9361 gcagcgcggg atcgcgccgc tgatggcgat cgcgcggccc gccgcgttcc tgcagttcgc
     9421 gctggtcgcg ctggcgttcg cgatcctcac ccacggtttc gtgatccatg atttttcgct
     9481 gcgctacgtc gcggagaatt cgaattcgct gctgccgatg gtgtaccgct acaccgcggt
     9541 gtggggcgcg cacgaaggct cgctgctgct gtgggcgctg atcctggccg cgtggaccgc
     9601 cgcggtcgcc ggattctcga gatcgttgcc gatgccggtg gtcgcgcgcg tgctgggggt
     9661 gatgggggtg gtcgcggtcg gcttcctcgc cttcctgatc ttcaccagca atccgttcga
     9721 gcgcctgctg ccggcggcgg cggatggccg cgacctcaac ccgctgctgc aggatccggg
     9781 gatgatcatc catccgccga tgctgtactt cggctacgtg ggtttcgcgg tgccgttcgc
     9841 gttcgcgatc gcggcgttgc tggagggccg ggtcgacgcg cagtggctgc gctggacgcg
     9901 gccgtggacg aatgtcgcgt gggggttcct gaccctgggc atcgcgattg gatcgtggtg
     9961 ggcgtactac gaactgggct ggggcggctg gtggttctgg gatccggtgg agaacgccag
    10021 cttcatgccg tggctggccg gcgcggcgct gctgcattcg caggcggtga ccgagaagcg
    10081 cggca
//
LOCUS       DAORUK010000070        10083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_7067_length_10083_cov_18.412545, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000070 DAORUK010000000
VERSION     DAORUK010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 10083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10083
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7067_length_10083_cov_18.412545"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="PK818_06190"
     CDS             <1..1202
                     /locus_tag="PK818_06190"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQY82358.1"
                     /translation="PNANYYTFYKVGSRNEYPGITGLAHYFEHMMFNGTTTRAPGEFD
                     RQMEAAGGSNNASTSNDLTIYTDWFPSSALETIFDLEGDRMANLAFDPKVVESERGVV
                     YSERRSSVDNDNFGTLMEQMQATAFTAHPYQFPTIGWPSDIEGWKLEDLKKFFRTYYA
                     PNNAVLVVAGDVEPAQVFALADKYLATLPKQPTPTPVTTVEPEQQGERRIVVERANAQ
                     SPIVAYAFHSGIGAGSTDFPALELLATILSQGQSSRLHQRLVEKEQAAVQAGAFAEGG
                     FDPGLLWVYAMLPPGGDMAKVEMLLDEELAKLARDGVTAAELDKARNLKASAFWRGMA
                     TINGKARALGTYEVFNGDHRKLFDAPAAFDAVTAADVQALAKKILRVQNRTTGLLKPV
                     AAMEAAK"
     gene            1199..2593
                     /locus_tag="PK818_06195"
     CDS             1199..2593
                     /locus_tag="PK818_06195"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQY82359.1"
                     /translation="MSVFRKACIAVVAMFAIGPAIATGIRLPDYQAITLDNGATVLLM
                     PRNDVPLVAASVAVRGGALADAAGKEGTADLLGGMLSKGAGNRDALQFAQALDGAGGS
                     LGFGSSREAITASAQFLSKDSALMLSLLADALLRPKMEQAEFDKLRKRAIDGIANAKD
                     SDPRQLIGTYAGAWLFRGHPYGRATGGDETSLATITLADLQAFRQQQMGGDRLVIAIA
                     GDFDASAMAAQVKQAFGGWGKAAGTLPQVEAKPRELGRRVLLVDKPGATQTYFALANI
                     GSRHGDPAEAAQDLVHTAFGGRFTSMLNTELRIKSGLSYGASAQLQRPRQPGASQIGS
                     FTKTESTKAAIDLAIATLDRLHRDGLDAATIDSAKRYVAGQFAPGLETAPQLAARLVE
                     MQLYGDSRDAVDGYLGRIAAATPAQVADARAVFPASADLAIVAIGDAAKIRAALQGYG
                     PLTEMKLTDPRFSP"
     gene            2684..4762
                     /locus_tag="PK818_06200"
     CDS             2684..4762
                     /locus_tag="PK818_06200"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HQY82360.1"
                     /translation="MLLSLAIAASLAACQKPAEPAATASAAAPAAPAAYTLDESKLPP
                     FNSFTLADLDPGKNACVDFAGHVNGKWLAANPIPGDRSSWGAFEMLAERSLAVQRQLA
                     EQAAAKADGAGVDKIVGDFWATGMDEAKINAQGLQPLAAELETIEKLQDVPELAHFLR
                     ASAATGNGYLIGIGAMPDFKDSSINIAAAGQGGLGLPDVPYYDKPEYQKIRDAYVAYI
                     TRMLELSGIPAADAAAQAKDVLAFETRLAKVSKSREALSRDVGLYYNPISPAEADKLS
                     PNFPFTKLFESQGVAVPAMFSLSVPDYHAEVSKMMADVPLPQWKSYLRFHLLDGVAPY
                     IADDFVQAHFEFYGKTLGGQKELRERGKRVLGTIDGQVGEALGQMYVEVAFPAESKAK
                     MQALIKNLSEALKLRIENLAWMGADTKKKALEKWASFEPRVGYPDKWREWTGLATSRD
                     SYIGNVLAARKFNYQWDIGKIGKPVDKTEWGMRPQTVNAQYNPLANQITFPAAILQPP
                     FFDPKADDALNYGGIVAVIGHEMTHGYDDQGSRFGPTGKFENWWTPADAEGFKARTGK
                     LVAQFDGYKAANGKNLNGNHTLGENIADLGGLATAYDAMVKASAGQPDPKIDGLTRDQ
                     RFFYSWATVWRRNFTPEELEQRLQTDEHALAEFRAIGAPSNMPAFATAFGCKAGDPML
                     RDGDRQVVIW"
     gene            4899..6806
                     /gene="dxs"
                     /locus_tag="PK818_06205"
     CDS             4899..6806
                     /gene="dxs"
                     /locus_tag="PK818_06205"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HQY82361.1"
                     /translation="MTDPTRYPLLSKVDSPADLRQLPESGLRAVADELRAYLIESVGK
                     SGGHFGAGLGVVELTTVLHYMYDTPDDRIVWDVGHQCYPHKILTGRRDRIHTVKQKDG
                     VAPFPKREESEFDTFGVGHSSTSISAALGMAIANARAGNPRKVVAVIGDGAMTAGMAY
                     EALNHAGGMDPEPDLLVILNDNRMSISEAVGGLTQMLGRMTGSKTLNALREGGKKLLG
                     DKNSPPARFVKRWEEHWKGMFVPSTLFEEMGFHYTGPIDGHDVDALLGVLKTLKTLKG
                     PQLLHVITTKGKGYELAEGDQIGYHAVSPFDPQTGVVAKAGASKPTYTDVFGDWLCDM
                     AAVDEQLLAITPAMREGSGLVRFSKEFPQRYFDVAIAEQHALTLAAGMACEGAKPVVA
                     IYSTFLQRGYDQLVHDVAIQDLDVLLAIDRGGVVGPDGATHAGNLDLSFLRCVPNMVV
                     MAPADENECRQLLTTGYRHTGPAAVRYPRGTGPGVAIQPSLDALPIGKADLRRNGSRI
                     ALLAFGAIVPAAQQVGEALDLTVVNMRFVKPLDRTLLLELAKTHDGFATLEDNVVAGG
                     AGSGVAELLAAEGISMPILHLGLPDAFQHHASREDLLAEAGLDAAGIRAAVLKRWPGL
                     AGSFRPQAATA"
     gene            complement(6895..7953)
                     /locus_tag="PK818_06210"
     CDS             complement(6895..7953)
                     /locus_tag="PK818_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HQY82362.1"
                     /translation="MRLAMLAVAVVGVMVALPLPLRAQQVDPQPEVPAAPIAKPQALP
                     GDGIRTEATVVVTGEQPGPGLWLVRKGNHDLWILGTLNPLPAGMQWQSKQVEDVIANA
                     QEVIRAPYVAFKVDTGFFKSLLLLPSMLGARKNPDGKTLQQVVSPQSYARWRALKARY
                     IGNDGGIEKWRPLFAAQELYKQAMKKSGLDNSKSVWPVINKAIETHHPNVTVVKEEIV
                     VKDPKPLLKEWSKTTLDDLACFDNTMTRIETDLDAMRARANAWATGDMAALQSLPSPY
                     QWEACSSAITEAGIGKRLGFGDAGQKVRTKWLAAAEAALDKNQVTFATLDMKDLLGAN
                     GFLARFKAKGYTVLAPDE"
     gene            complement(8085..8161)
                     /locus_tag="PK818_06215"
     tRNA            complement(8085..8161)
                     /locus_tag="PK818_06215"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8125..8127),aa:Pro,seq:ggg)
     gene            complement(8197..8553)
                     /locus_tag="PK818_06220"
     CDS             complement(8197..8553)
                     /locus_tag="PK818_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQY82363.1"
                     /translation="MLDPGSNRELPPIPAKRYFTIGEVSELCDVKPHVLRYWETEFPS
                     LEPAKRRGNRRYYQRHDVLMVRQIRSLLYEQGYTIGGARLRLDGDDARQESALSNQIA
                     KQVRMELEEVLQLLRR"
     gene            complement(8546..8848)
                     /locus_tag="PK818_06225"
     CDS             complement(8546..8848)
                     /locus_tag="PK818_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HQY82364.1"
                     /translation="MALTKAEMAERLYEDVGLNKREAKEFVDAFFDALREALEHGRQV
                     KLSGFGNFDLRRKNQRPGRNPKTGQEIPISARTVVTFRPGQKLKERVEAYVGPAHA"
     gene            complement(8860..>10083)
                     /locus_tag="PK818_06230"
     CDS             complement(8860..>10083)
                     /locus_tag="PK818_06230"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HQY82365.1"
                     /translation="AGGAPGPVIEAVLPQHLPRPQAIRLRRARLARVLGTQVADAEVS
                     RILNALGLAVEATAEGWSVTPPARRFDLAIEEDLIEEIARIHGYDAIPATLPGGATRL
                     VSPSETCSSEHDARRQLVARDYLEAINYAFVDADLLAKWGLADGGVALANPLSAELGV
                     MRTRLLPGLVAALGRNAARQQTRVRLFELGKTFAASDDAPVETRRIAAVACGDATGEQ
                     WGAGARKLDFHDLKGDLDSLAALSGAVLEYRASVQPFAHPGRCADVLRDGACIGWIGQ
                     LHPRLQQALDVDAEVFGFEVALDALLARPVPRARALSKYPSVRRDLAFVVPESVTWDA
                     MRATAGQAAGPALRELRLFDRYAGRGVETGFKSLAMALILQDETRTLTDRDVEAVVAG
                     VVAALEQHHGATIRR"
BASE COUNT         1680 a         3604 c         3328 g         1471 t
ORIGIN      
        1 tccccaacgc caactactac accttctaca aggtcggcag ccgcaacgaa taccccggca
       61 tcaccgggct cgcgcactac ttcgagcaca tgatgttcaa cggcaccacc acccgcgccc
      121 cgggcgagtt cgacaggcag atggaagccg ccggcggcag caacaatgcc agcaccagca
      181 acgacctgac catctacacc gactggttcc cgagcagcgc gctggagacg atcttcgacc
      241 tcgaaggcga ccgcatggcc aacctggcgt tcgatcccaa ggtggtggaa agcgagcgcg
      301 gcgtggtcta cagcgagcgt cgctccagcg tggacaacga caatttcggc accctgatgg
      361 agcagatgca ggcgaccgcc ttcaccgcgc acccctacca gttcccgacc atcggctggc
      421 cgtccgacat cgaaggctgg aagctcgagg acctgaagaa attcttcagg acctattacg
      481 cgccgaacaa cgcggtgctg gtggtggccg gcgacgtcga accggcgcag gtgttcgcgc
      541 tggccgacaa gtacctggcc acgctgccga agcagcccac gccgaccccg gtcaccacgg
      601 tcgagccgga acagcagggc gagcgccgga tcgtggtgga gcgcgccaac gcgcagtcgc
      661 cgatcgtcgc ctacgccttc cacagcggca tcggcgcggg cagcacggac ttcccggcgc
      721 tggagctgct ggccaccatc ctgtcgcagg gccagtcctc gcgcctgcac cagcgcctgg
      781 tcgagaagga acaggccgcc gtgcaggccg gcgccttcgc cgagggcggc ttcgacccag
      841 gcctgctgtg ggtgtacgcg atgctgccgc ccggcggcga catggcgaag gtggaaatgc
      901 tgctggacga ggaactggcg aagctcgcgc gcgacggcgt gactgcggcg gaactggaca
      961 aggcacgaaa cctgaaggcg tccgcgttct ggcgcggcat ggccaccatc aacggcaagg
     1021 cgcgcgcgct tggcacctac gaggtgttca acggcgacca tcggaaactg ttcgacgcac
     1081 ccgccgcgtt cgatgcagtc accgccgccg acgtgcaggc gctggcgaag aagatcctgc
     1141 gcgtgcagaa ccgcaccacc ggcctgctga agccggttgc cgccatggag gccgcgaaat
     1201 gagtgtcttc cgaaaggctt gcatcgcagt ggtcgcgatg ttcgcgatcg gtccggcgat
     1261 cgccaccggg atcaggctcc ccgattacca ggccatcacc ctcgacaacg gcgccaccgt
     1321 gctgctgatg ccgcgcaacg acgtgccgct ggttgccgcc agcgtcgccg tgcgcggtgg
     1381 cgcgcttgcg gatgccgcag gcaaggaagg caccgccgac ctgcttggcg ggatgctgtc
     1441 gaagggcgcc ggcaaccgcg atgcactgca gttcgcgcag gcgctcgatg gcgccggcgg
     1501 cagcctcggc ttcggcagct cgcgcgaggc aatcaccgcc agcgcgcagt tcctgtccaa
     1561 ggacagcgcg ctgatgctgt cgctgctcgc ggacgccttg ctgcggccga agatggagca
     1621 ggccgagttc gacaagctgc gcaagcgcgc catcgacggc atcgccaacg ccaaggattc
     1681 cgacccgcgc cagctgatcg gcacctacgc gggcgcgtgg ctgttccgcg gccatcccta
     1741 cgggcgcgcc accggcggcg atgaaaccag cctcgccacc atcaccctcg ccgacctgca
     1801 ggcgttccgc cagcagcaga tgggcggcga ccgcctggtc atcgccatcg ccggcgactt
     1861 cgacgcctcc gcgatggccg cgcaggtcaa gcaggcattc ggtggctggg gcaaggccgc
     1921 cggcacgctg ccgcaggtcg aggcgaagcc gcgcgaactc ggccgtcggg tgctgctggt
     1981 cgacaagccc ggcgccaccc agacctactt cgcgctggcc aacatcggca gccgccacgg
     2041 cgatccggcc gaggctgcac aggacctggt gcacaccgca ttcggcgggc gcttcacctc
     2101 gatgctcaac accgagctgc ggatcaagtc cggcctcagc tatggcgcca gcgcgcagct
     2161 gcaacggcca cgccagccgg gtgcatcgca gatcggcagc ttcaccaaga ccgaatcgac
     2221 caaggccgcg atcgacctcg ccatcgccac cctcgaccgc ctgcaccggg acgggctgga
     2281 cgcggcgacc atcgactccg ccaagcgcta tgtcgccggc cagttcgcgc cgggcctgga
     2341 aaccgcgccg caactggccg cccggctggt cgagatgcag ctgtacgggg attcgcgcga
     2401 cgcggtcgac ggctaccttg gccggatcgc cgcggccacc ccggcgcagg tcgcggatgc
     2461 gcgcgcggtg ttcccggcca gcgcggacct ggccatcgtc gccatcggcg atgccgcgaa
     2521 gatccgcgcc gcgctgcagg gctatggccc gctgaccgaa atgaagctga ccgatccgcg
     2581 cttctcgccc tgaccattgc tgcagtcatc gccgcggttg ccgcgatgac ccgcaggaca
     2641 cccactcccg aacctggagc ctcacgtgaa aaccccgaag atcctgctgt tgtcgctcgc
     2701 catcgccgcc agcctggccg cctgccagaa gccggcggaa cccgccgcca cggccagcgc
     2761 cgccgccccg gccgcgccgg cggcgtacac cctggatgaa tccaagctgc cgccgttcaa
     2821 cagcttcacc ctcgccgacc tcgacccggg caagaacgcc tgcgtggact tcgccgggca
     2881 cgtcaacggc aagtggctcg ccgccaaccc gatcccgggc gaccgcagca gctggggcgc
     2941 gttcgagatg ctggccgagc gttcgctggc ggtgcagcgc cagctcgccg agcaggccgc
     3001 ggccaaggcc gatggcgccg gcgtcgacaa gatcgtcggc gacttctggg ccaccggcat
     3061 ggacgaggcg aagatcaacg cgcagggcct gcagccgctt gccgccgaac tggagacgat
     3121 cgagaagctg caggacgtcc ccgagcttgc ccatttcctg cgtgcgtccg cggccaccgg
     3181 caatggctac ctgatcggga tcggggcgat gccggacttc aaggactcca gcatcaacat
     3241 cgccgccgcc ggccagggtg gcctgggcct gccggacgtg ccgtattacg acaagcccga
     3301 gtaccagaag atccgcgacg cctacgtggc ctacatcacc aggatgcttg agctgtccgg
     3361 catcccggcc gccgatgcgg cggcgcaggc gaaggacgta ctggcgttcg agacccgcct
     3421 ggccaaggtc agcaagtcgc gcgaggcgct ctcgcgcgat gtcggcctgt actacaaccc
     3481 gatcagcccg gccgaggccg acaagctgtc cccgaacttc cccttcacca agctgttcga
     3541 gtcgcagggc gtcgcggtgc cggcgatgtt ctcgctgtcg gtgccggact accacgcgga
     3601 ggtcagcaag atgatggccg acgtgccgtt gccgcagtgg aagagctacc tgcgcttcca
     3661 cctgctggac ggcgtggcgc cctacatcgc cgacgatttc gtgcaggccc acttcgagtt
     3721 ctacggcaag accctgggcg ggcagaagga gctgcgcgaa cgcggcaagc gcgtgctcgg
     3781 caccatcgat ggccaggtcg gcgaggcgct cggccagatg tacgtggagg tggcgttccc
     3841 ggccgagtcc aaggccaaga tgcaggcgct gatcaagaac ctcagcgagg cgctgaagct
     3901 gcgcatcgag aacctggcct ggatgggcgc ggacaccaag aagaaggcac tggagaagtg
     3961 ggcctcgttc gaaccgcgcg tcggctaccc ggacaagtgg cgcgagtgga ccggcctggc
     4021 caccagccgc gacagctaca tcggcaacgt gctggccgcg cgcaagttca actaccagtg
     4081 ggacatcggc aagatcggca agccggtgga caagaccgaa tggggcatgc gcccgcagac
     4141 ggtcaacgcg cagtacaacc cgctggccaa ccagatcacc ttcccggccg cgatcctgca
     4201 accgccgttc ttcgacccga aggcggacga cgcgctgaac tatggcggca tcgtcgcggt
     4261 gatcggccac gagatgaccc acggctacga cgaccagggc agccgcttcg gcccgaccgg
     4321 caagttcgag aactggtgga ccccggccga cgccgagggc ttcaaggccc gtaccggcaa
     4381 gctggtcgcg cagttcgacg gctacaaggc ggccaacggc aagaacctca acggcaacca
     4441 caccctgggc gagaacatcg ccgacctcgg cggcctcgcc accgcctacg acgcgatggt
     4501 caaggccagc gccggccagc cggatccgaa gatcgacggc ctcacccgcg accagcgctt
     4561 cttctacagc tgggccaccg tgtggcgccg caacttcacc ccggaggaac tggagcagcg
     4621 cctgcagacc gacgagcacg cgcttgccga gttccgcgcg atcggcgcgc cgtccaacat
     4681 gccggcgttc gccaccgcgt tcggctgcaa ggccggcgac ccgatgctgc gcgatggcga
     4741 caggcaggtc gtgatctggt gatccccgcc tgacgttgcc gcggccccgc tgcggttcgc
     4801 cagctcgaag gcccggcacg tccgggcctt cttcttttcc ggcggccggg tgcgggttct
     4861 tcattgcccg ccaccccgcc cggcgcgaca ataggccgat gaccgacccc acccgctacc
     4921 ccctgctgtc gaaggtcgac agcccggccg acctgcgcca gctgcccgaa tccgggctgc
     4981 gcgcggtggc cgacgagctg cgcgcctacc tgatcgaatc ggtcggaaaa tccggtggcc
     5041 atttcggcgc cggcctcggc gtggtcgagc tgaccacggt gctgcattac atgtacgaca
     5101 ccccggatga ccgcatcgtc tgggatgtcg gccaccagtg ctacccgcac aagatcctca
     5161 ccggacgccg cgaccgcatc cacacggtca agcagaagga cggcgtggcg ccgttcccga
     5221 agcgcgagga aagcgaattc gacaccttcg gcgtcggcca ttcctcgacc tcgatctcgg
     5281 ccgcgctcgg catggcgatc gccaatgccc gcgccggaaa cccgcgcaag gtggtggcgg
     5341 tgatcggcga cggcgcgatg accgccggca tggcctacga ggccctgaac cacgccggcg
     5401 gcatggaccc cgaaccggac ctgctggtga tcctcaacga caaccggatg tcgatcagcg
     5461 aagcggtggg cggcctcacc cagatgctcg ggcgcatgac cggcagcaag accctgaacg
     5521 cgctgcgcga aggcggcaag aagctgctcg gcgacaagaa cagcccgccg gcgcgcttcg
     5581 tcaagcgctg ggaggagcac tggaagggca tgttcgtgcc ctccaccctg ttcgaggaaa
     5641 tgggcttcca ctacaccggc ccgatcgacg gccacgacgt cgacgccctg ctcggcgtgc
     5701 tgaagaccct gaagacgctg aagggcccgc agctgctgca cgtgatcacc accaagggca
     5761 agggctacga actggccgaa ggcgaccaga tcggctacca cgcggtgtcg ccgttcgatc
     5821 cgcagaccgg cgtggtcgcc aaggccggtg cgtcgaagcc gacctacacc gacgtgttcg
     5881 gcgactggct gtgcgacatg gccgccgtcg acgagcagct gctcgcgatc accccggcga
     5941 tgcgcgaagg ctccggcctg gtgcgtttca gcaaggaatt cccgcagcgc tacttcgatg
     6001 tcgccatcgc cgagcagcat gcgctcaccc ttgccgccgg catggcctgc gaaggcgcca
     6061 aaccggtagt cgcgatctat tcgaccttcc tgcagcgcgg ctacgaccag ctggtgcacg
     6121 acgtcgcgat ccaggacctc gacgtgctgc tggcgatcga ccgcggcggc gtggtcgggc
     6181 cggatggcgc cacccacgcc ggcaacctgg acctcagctt cctgcgctgc gtgccgaaca
     6241 tggtggtgat ggccccggcc gacgaaaacg aatgccggca actgctgacc accggataca
     6301 ggcacacggg cccggcggcg gtgcgttacc cgcgcggcac cggccccggc gtggcgatcc
     6361 agccatcgct ggacgcgctg ccgatcggca aggcggacct gcgccgcaac ggctcgcgca
     6421 tcgccctgct cgcgttcggc gccatcgtgc cggcggcgca acaggtcggc gaagcgctgg
     6481 acctcaccgt ggtcaacatg cgtttcgtga agccgctgga tcgcaccctg ttgctcgagc
     6541 tggcgaagac ccacgacggc ttcgccaccc tcgaggacaa cgtggtggcg ggtggcgccg
     6601 gcagcggcgt ggccgagttg ctggcggcgg aaggcatcag catgccgatc ctgcacctgg
     6661 gcctgccgga tgcgttccag caccacgcca gccgcgagga cctgctggcc gaagccgggc
     6721 tggacgccgc cggcatccgc gcggcggtac tcaagcgttg gcccggactg gccgggtctt
     6781 tccgcccaca agccgcgacg gcctgaaatc ccttgcagga gcggcgcaag ccgcgacctg
     6841 gctgcgtccg gacgcatggt cgcggctgac gccgcacctg cagcagccgc cggctcattc
     6901 gtccggcgcc agcaccgtgt agcccttggc cttgaacctg gccaggaagc cgttggcacc
     6961 gagcaggtcc ttcatgtcga gggtggcgaa ggtcacctgg ttcttgtcga gcgcagcctc
     7021 ggcggcggcc agccacttgg tccgcacctt ctggccggca tcgccgaagc cgagccgctt
     7081 gccgatcccg gcctcggtga tcgcgctgct gcacgcttcc cactggtagg gcgacggcag
     7141 cgactgcagc gcggccatgt cgccggtggc ccacgcgttc gcgcgcgcgc gcatggcgtc
     7201 gagatcggtt tcgatgcggg tcatcgtgtt gtcgaagcag gcgaggtcgt cgagcgtggt
     7261 cttcgaccat tccttcagca acggcttcgg gtccttgacc acgatctcct ccttcaccac
     7321 cgtcacgttc gggtggtggg tctcgatcgc cttgttgatc accggccaga ccgacttgga
     7381 attatcgagg ccggatttct tcatcgcctg cttgtacagc tcctgcgcgg cgaacaacgg
     7441 gcgccacttc tcgatgccgc cgtcgttgcc gatgtagcgc gccttcagcg ccctccagcg
     7501 cgcgtaggac tgcggcgaga caacctgctg cagggtcttg ccatccgggt tcttgcgcgc
     7561 gccgagcatc gacggcagca gcaacaggct cttgaagaat ccggtgtcca ccttgaaggc
     7621 cacgtacggc gcgcggatca cttcctgtgc attggcgatc acgtcctcga cctgcttcga
     7681 ctgccactgc atgccggccg ggagcggatt gagcgtgccc aggatccaca ggtcgtggtt
     7741 gcccttgcgc accagccaca ggcccgggcc cggctgctcg ccggtcacca ccaccgtggc
     7801 ttcggtgcgg atgccgtcgc caggcaacgc ctgcggcttg gcgatcggcg cagccggaac
     7861 ctccggctgc gggtccactt gctgcgcgcg cagcggcagc ggcaacgcca ccatcacacc
     7921 caccaccgcc accgcgagca tcgccaagcg catcctgcac gtccttgctg aagcggcgtc
     7981 agcgccgcga cgcagtgtga ccgggcgcac accgggaagt tcaaccgcgc actgaacgcg
     8041 catagcagaa cgcccggcaa gccgggcgtt cgagacttcg cgattggtcg ggacggccgg
     8101 atttgaaccg acgaccctct gccccccagg cagatgcgct accaggctgc gctacgcccc
     8161 gatgtcgcga acgtcattgt aaccgctcgg cgcgcttcag cggcgcagca gttgcaggac
     8221 ttcttccagt tccatccgca cctgcttggc gatctggttg ctcaacgccg attcctgcct
     8281 ggcgtcgtcg ccatccaggc gcaggcgcgc gccaccgatg gtgtagccct gctcgtacag
     8341 caggctgcgg atctggcgca ccatcagcac gtcgtggcgc tggtagtagc ggcggttgcc
     8401 gcggcgcttg gccggctcca ggctggggaa ttcggtttcc cagtagcgca gcacgtgcgg
     8461 cttgacgtcg cacagctcgc tgacctcgcc gatggtgaag tagcgcttgg ccgggatcgg
     8521 cgggagttcg cggttgctgc cggggtcaag catgggccgg ccccacgtag gcttccaccc
     8581 gctccttgag cttctggccc gggcggaacg tgacgacagt gcgtgccgag atcgggattt
     8641 cctggccggt cttggggttg cggccgggtc gctggttctt gcgccgcagg tcgaaattgc
     8701 cgaaaccgga cagcttgacc tggcggccgt gctccagcgc ctcgcgcaac gcatcgaaga
     8761 acgcatcgac gaattccttc gcctcgcgct tgttcaggcc cacgtcctcg tacagacgtt
     8821 ccgccatttc cgccttggtc aacgccattg ccgtcccctt cagcgccgga tggtcgcgcc
     8881 gtggtgctgc tccagcgcgg caaccacccc ggccaccacc gcttccacgt cgcggtctgt
     8941 cagggtgcgc gtttcatcct gcaaaatcaa ggccatggcg agactcttga atccagtttc
     9001 cacgcccctg ccggcgtagc ggtcgaacag ccgcagctcg cgcagggcgg ggcccgcggc
     9061 ctgcccggcg gtggcccgca tggcatccca ggtcaccgac tccggcacca cgaacgccag
     9121 gtcgcggcgc accgacggat acttggacaa cgcccgcgcg cgcggcaccg ggcgcgccag
     9181 caacgcatcc agcgccactt cgaagccgaa cacctcggca tcgacgtcca gcgcctgctg
     9241 caggcgcgga tgcagctggc cgatccagcc gatgcaggca ccgtcgcgca gcacgtccgc
     9301 gcagcggccg ggatgcgcga acggctgcac cgacgcgcgg tactccagca ccgcgccgga
     9361 cagcgcggcg aggctgtcga ggtcgccctt gaggtcgtgg aaatcgagct tgcgcgcgcc
     9421 cgcgccccac tgctcgcccg tcgcatcgcc acaggcgacc gccgcgatgc gtcgcgtctc
     9481 gaccggcgcg tcgtcgctgg cagcgaaggt cttgccgagc tcgaacaggc gcacccgcgt
     9541 ctgctggcgc gcggcattgc ggccgagcgc ggcgaccagt ccgggcaaca ggcgcgtgcg
     9601 catcacgccc agttcggcgc tcagcgggtt ggccagcgca acgccgccat cggcgagacc
     9661 ccactttgcg agcaggtcgg catcgacgaa ggcgtaattg atcgcctcca ggtagtcgcg
     9721 cgcgaccagc tggcggcgag cgtcgtgctc gctgctgcaa gtctcgctgg gcgaaaccag
     9781 gcgcgtggcg ccgcccggca gggtggccgg gatcgcgtca tagccgtgga tgcgggcgat
     9841 ctcctcgatc aggtcctctt cgatcgcgag gtcgaagcgg cgcgccggcg gtgtcacgct
     9901 ccagccctcc gcggtggctt ccaccgcaag gcccagggca ttgaggatgc gggagacttc
     9961 ggcatcggcc acctgggtgc cgagcacgcg cgccagccgc gcacgacgca agcggatcgc
    10021 ctgcggccgc ggcaggtgct gcggcagcac ggcctcgatc accgggcccg gcgcgccacc
    10081 ggc
//
LOCUS       DAORUK010000071         9972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_7174_length_9972_cov_20.934153, whole genome shotgun sequence.
ACCESSION   DAORUK010000071 DAORUK010000000
VERSION     DAORUK010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 9972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9972
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7174_length_9972_cov_20.934153"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PK818_06235"
     CDS             complement(<1..652)
                     /locus_tag="PK818_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82366.1"
                     /translation="MKTASLATALVAALLLGACKGDDPQAAAQAAAAQAAAREQVAEG
                     MAKQFDDAVAAQNWQLAKAHGEILQIEHPASAAAARVKPALDEIKAKAEAAQQEKRLA
                     ALWAYGDEPVGKDGHQLSAAIFSQEPVDTDGSGANPVRLIFRDHPEWGRSAYLVLEAG
                     DFDCYSGCRLKVIADGKTHALPGSRPNTDEAIAMFIDDHKALWKLAKAGKQLSIEFP"
     gene            complement(672..1658)
                     /locus_tag="PK818_06240"
     CDS             complement(672..1658)
                     /locus_tag="PK818_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQY82367.1"
                     /translation="MARASLRRRGIGLLLLAVVATATAVLWRDPFLLVRGEFVRQRIV
                     AGLSRGTVEAAGHRWVYAQREAGGGDAPTIVMLHGYTGSKENWYPLARELDGRYRLLI
                     PDLPGWGESERKPGAVYGFPEQAANVAAFIRATSPGRPVLLLGHSMGGGIAALAAARY
                     PREIAQVGLLDAAGVRFRENPFGLDVLAGGNPFAVDDEASLRRYIDIVFHDANAKPWL
                     PWPATTGLIRKRRADAAFEQGVLDRIGRSSESLLPGEEAARIRQPALLLWCRQDAVID
                     ASALDLYAQRIPQARKVLLEGCGHMSLMERPREVADAVVALLENGENAGDAE"
     gene            complement(1665..3710)
                     /locus_tag="PK818_06245"
     CDS             complement(1665..3710)
                     /locus_tag="PK818_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQY82368.1"
                     /translation="MRQLLLSASIAAALGLVATGCARSPGPEPSSATPAAQPAAVAET
                     ITDEHSYAEPDKVRTTDLALDLAVDFGTKTLTGTATYSLAWLDKDAAQLVLDTRDISI
                     RKAEGLSAEGQWSELKFALADEDPILGSKLTIEAPEHPTQVRVTYSTSPEASGLQWLE
                     PSMTEGKKTPFMFSQSQQIHARSWVPLQDTPSIRFTYSAHVTAPKDAMVLMSADNDPA
                     AARDGDYSFKMPQKIPSYLLAIAAGDLVFKPIGERSGVWAEPSMVDKAVAEFIDTEKM
                     IETAEQMYGPYRWGRYDLLVLPPSFPYGGMENPRLTFATPTVIVGDKSLVSLVAHELA
                     HSWSGNLVTFATDKDAWLNEGFTSYVENRLVEALFGKEQADMETAIGRAEVIEEYKTL
                     EPKLQRLALKPGDLADPDNSSSATVYQKGAWFLSFLENRFGREVFDGWLKGYFDRHAF
                     QGMTTTRLVEDLKQNLLAKNPGKVTEAEVIEWVYEPGIPANAPKVESRRFSTVDAARI
                     AWTGSGMLADKSITDAWSTQEWVRFLEGMPDALKPEQLRQLDASYMLTGTPNGEIAQR
                     WYPLAERSGYAEARAEMGKFLERVGRRKLIMPTYKALAETQDGLAFARQVFAKAKPGY
                     HPITTGSVQAVIDAATAKADSLPATAAPARPAAAGQTMQVPETLHPSDPVPPPAETQ"
     gene            3872..6127
                     /locus_tag="PK818_06250"
     CDS             3872..6127
                     /locus_tag="PK818_06250"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HQY82369.1"
                     /translation="MHLPRRGALALALACLIAPCAPAFSQTPAAATAGFVPGGRIPRL
                     QDGIVIDGVLDDAAWAGALVQEIGYDIQPGDNTPAPVKTTVRIGYTTDALYLSYRALD
                     PDPASIRAHLRDRDSAFNDDWVGVFMDTFDDQRRGYEFIVNPLGVQADLINDATTGNE
                     DPSWDGLWSSAGRITADGYEVEIRIPFSTLRFRDGGGDKRWGISLFRNWPRDKRHQLT
                     SHRVPRESNCFLCEWTKYDGMAGVQQGRNIEVVPTLTMGASQSRDAAGTPWASGDNSI
                     EPGLDVSWAPSPDMTLNATLNPDFSQVETDQLQLDINNSFALFYQEKRPFFLEGADYF
                     TTQFDVLYTRQVADPDFGLRTTGRTGSGAYGAIVARDASTLVLVPGVLGSRFVQLDQP
                     ANVAVGRYRHDLDRHTSIGVIGTFREGDGYANNVAGVDARWQQGAHTASAQWLHSQSE
                     YPEQVVDVYKGELGDDPTPAGNAWRAQYSFNNRNWNASVQRWVIDPGFRADLGFMGQV
                     GYDKSVAGGGYSWFRDGKAVNRINLYADWDITHRFDGQLLERELEFSLGFQGPKQSQF
                     GINPLGRVRYWRGAMFNEHNLNLYGNFRPMADLQLGTYMNFGQQLDLRAARTGRRQML
                     GLWGNANAGRGLTMDWDISRQRLRRDGGIAFEASLVNAGGSWQFDPRQRIRLTLQGSE
                     VLRDQSLYGTHVNEAARDWAAQAVYSYKVNPRTAVYAGASYGAFMDDDNQDLFGATRS
                     VFVKLSYGWQP"
     gene            complement(6134..6322)
                     /locus_tag="PK818_06255"
     CDS             complement(6134..6322)
                     /locus_tag="PK818_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82370.1"
                     /translation="MSRPPLKTFRDSRWRYSQFVVLGLVVAGLVKWLSPFGWPPSLLA
                     GAVVAAGYLLFEKKRGVI"
     gene            complement(6327..7088)
                     /gene="ubiE"
                     /locus_tag="PK818_06260"
     CDS             complement(6327..7088)
                     /gene="ubiE"
                     /locus_tag="PK818_06260"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HQY82371.1"
                     /translation="MSESTGKPGTTHFGFRDVPVAEKQKLVGEVFSSVAGNYDLMNDA
                     MSLGIHRLWKRYFVATAQVRKGDRVLDLAGGTGDIAALLKERVGEGGELVLGDINGEM
                     LRVGRDRMTDRGNVRGFEYVQCNAESLPFPDASFDLVTIAFGLRNVTDKDAALREMLR
                     VLRPGGQARVLEFSEVQAEWFRPIYDFHSFQVLPRLGKLFAGDAGSYQYLAESIRKHP
                     PQAELKAMMEAAGFARCDYRNLNAGIVAIHTGYKA"
     gene            7178..8866
                     /locus_tag="PK818_06265"
     CDS             7178..8866
                     /locus_tag="PK818_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HQY82372.1"
                     /translation="MPDHPARAAVLPALIADDAALDRYLGEFQRTAYAMQRTQAGISM
                     ADWLATGEDACERCWLHAQRLDVPLVRLPQVSADPATACLLRPEQARRLRVVPLRAAH
                     GLLAVAMEDPSDSDALAAVEFLSGNRVLPLMATARGIREAIARSYDQVEDRETVRLLG
                     LDPGASVDTSESEALRLSREQPVVRIVHGLIADAVARRASDIHLRPGERGTDVLFRID
                     DELVPVRSLLRALHAAVVSRIKVLGGMNLAEHRRPQDGRTSFTLDDGTRVDLRVSVLP
                     AVFGESVVVRLLDTRESLWNLEQLGLDDTDRRRIDDVMSRSHGMFLATGPTGCGKSTT
                     LYAMLMELRRQRINILTVEDPVEFHIAEIQQMQVNRAAGFTFASAMRNFLRHDPDVIM
                     VGEIRDRETADIAVESALTGHLLLSTLHTNTAATTITRLLDLGVEAYLLRASLLAVIS
                     QRLVRLTCPHCREVETVDPHVRAALGVGADEVFHRGHGCSQCEGLGVYKRQAVYEFLV
                     MSPRLRELVVPGAEADTIHRVAVEEGMRPITQAAVALARSGAISLAEAWRVRAD"
     gene            8902..9642
                     /locus_tag="PK818_06270"
     CDS             8902..9642
                     /locus_tag="PK818_06270"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HQY82373.1"
                     /translation="MRTPPNRIHLVLRIDGEAVPVSAPRPPLRARLDELLPAQRAVDN
                     AAIDHAVRKAAAAGKQVTCAKGCSACCRAQPVPVTPAEAYALLRLVEALPAARREQVE
                     ARFDDRVQRLRGAGLADLLLDRADDLDAERARGLALAYFKLGLVCPFLAEDACSIHPQ
                     RPFVCRQYLVSSDPALCADPFANHAEVIPMPLHAATAALAISETAYGRAQHSVPLTLA
                     LEYARRHRAELERQFDAEPLFRQWLGAL"
     gene            complement(9662..>9972)
                     /locus_tag="PK818_06275"
     CDS             complement(9662..>9972)
                     /locus_tag="PK818_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82374.1"
                     /translation="GRRLPLRHALPQPSSASMVIPPPKSVPRQANLVALAKMLRIDPR
                     ALQYGDPDGRNIREPGKAWKVTAADQLAIDAFLALPSAQRKAIRDLIATLARAQATAA
                     "
BASE COUNT         1501 a         3624 c         3331 g         1516 t
ORIGIN      
        1 tcgggaattc gatcgacagc tgcttgccgg ccttggcgag tttccacagc gccttgtggt
       61 cgtcgatgaa catcgcgatc gcctcgtcgg tgttcggccg cgaaccgggc aaggcatgcg
      121 tcttgccgtc ggcgatgacc ttcagccggc agcccgagta gcagtcgaag tcgccggcct
      181 ccagcaccag gtaggcgctg cggccccact ccggatggtc gcggaagatc agccgcaccg
      241 ggttggcacc gctgccgtcg gtgtcgaccg gctcctgcga gaagatcgcg gccgacagct
      301 ggtggccatc cttgccgacg ggttcatcgc catacgccca cagcgcggcc agccgcttct
      361 cctgctgcgc ggcctcggcc ttggccttga tttcgtccag cgccggcttg acccgcgcgg
      421 cggcggcgct ggcgggatgc tcgatctgca ggatctcgcc gtgggccttg gccagctgcc
      481 agttctgcgc ggccaccgcg tcgtcgaact gcttcgccat gccttcggcc acctgctccc
      541 tggccgccgc ctgcgcggcc gcggcctgcg ccgcggcctg cgggtcgtcg cccttgcagg
      601 cgcccagcag cagtgccgcg acgagggcgg tggcgagcga tgcggtcttc atcccgggat
      661 tcctgtgcgg ctcattcggc gtcgcccgca ttctcgccat tttccagcag cgcgaccacg
      721 gcatcggcca cttcgcgcgg gcgctccatc agcgacatgt gcccgcagcc ttccagcagc
      781 accttgcgcg cctgcgggat gcgctgcgcg tacaggtcca gggcactggc atcgatcacc
      841 gcgtcctgcc ggcaccacag cagcagcgcg ggctggcgga tgcgcgcggc ctcttcgccc
      901 ggcagcaacg actcgctgct gcggccgatc cggtccagca cgccctgctc gaatgcggca
      961 tccgcgcggc gcttgcggat cagcccggtg gtcgccggcc acggcagcca tggcttggcg
     1021 ttggcgtcat ggaagacgat gtcgatgtag cgccgcagcg aggcctcatc gtccaccgcg
     1081 aacggattcc cgccggccag cacgtccagc ccgaacgggt tctcgcggaa gcgtacgcca
     1141 gccgcatcca gcaagccgac ttgcgcgatc tcccgcggat accgcgcggc ggcgagcgcg
     1201 gcgatgccgc cgcccatcga atgtcccagc agcagcaccg gccggccggg tgaggtcgcg
     1261 cggatgaagg ccgccacgtt cgccgcctgc tccgggaagc cgtacaccgc gccgggcttg
     1321 cgttcggact cgccccagcc cggcaggtcc gggatcagca ggcgatagcg cccgtcgagt
     1381 tcgcgcgcca gcggatacca gttctccttg ctgccggtgt agccgtgcag catcacgatg
     1441 gtgggcgcat ccccgccacc ggcctcgcgc tgcgcgtaca cccagcgatg cccggccgcc
     1501 tcgacggtgc cgcgcgacag cccggcgacg atccgctggc gcacgaattc gccgcgcacc
     1561 agcaggaacg gatcgcgcca caacacggcc gtggcggtgg ccacgaccgc cagcagcagc
     1621 aagccgatcc cgcgccgccg cagcgatgcc cgcgccatcg gcgcttactg ggtttcggcg
     1681 ggcggcggca ccgggtcgga cggatgcagg gtttccggca cctgcatggt ctgtccggca
     1741 gcagcggggc gggccggcgc ggccgtggcc ggcaggctgt cggccttcgc ggtggcggcg
     1801 tcgatcaccg cctgcaccga gccggtggtg atcgggtggt aacccggctt cgccttcgcg
     1861 aacacctgcc gggcgaaggc caggccgtcc tgggtttcgg ccagtgcctt gtaggtcggc
     1921 atgatcagct tgcgacggcc aacccgctcc aggaacttgc ccatctccgc gcgcgcctcg
     1981 gcgtagccgc tgcgctcggc cagcggatac cagcgctggg cgatctcgcc gttcggggtg
     2041 ccggtcagca tgtaggacgc gtccagctgc cgcagctgct ccggcttcag cgcatccggc
     2101 atgccctcca ggaagcgcac ccattcctgg gtgctccagg catcggtgat gctcttgtcc
     2161 gccagcatgc cgctgccggt ccacgcgatc cgcgcggcat ccacggtgct gaaacggcgc
     2221 gactccacct tcggcgcatt cgccgggatg ccgggctcgt acacccattc gatgacctcg
     2281 gcttcggtca ccttgcccgg gttcttcgcc agcaggttct gcttgaggtc ctccaccagc
     2341 cgggtggtgg tcatgccctg gaacgcatgg cggtcgaagt agcccttcag ccacccatcg
     2401 aacacctcgc ggccgaagcg gttctccagg aacgacagga accacgcgcc cttctggtac
     2461 acggtggcac tggaggaatt gtcgggatcg gcgaggtcgc ccggcttcag cgcgaggcgc
     2521 tgcagcttcg gttccagggt cttgtattcc tcgatcacct cggcgcggcc gatcgcggtt
     2581 tccatgtcgg cctgttcctt gccgaacagg gcctccacca ggcggttctc cacgtagctg
     2641 gtgaagcctt cgttgagcca cgcgtccttg tcggtggcga aggtgaccag gttgcccgac
     2701 cagctgtgcg caagctcgtg cgcgaccagc gacaccagcg acttgtcgcc gacgatcacg
     2761 gtgggggtgg cgaaggtcag gcgcgggttt tccatgccgc cgtacgggaa gctcggcggc
     2821 agcaccagca ggtcatagcg gccccagcgg tacgggccgt acatctgctc ggcggtttcg
     2881 atcatcttct cggtatcgat gaactcggcc accgccttgt ccaccatcga cggctccgcc
     2941 cacacgccgc tgcgctcgcc gatcggcttg aacaccaggt cgccggcggc gatcgccagc
     3001 aggtaggacg ggatcttctg cggcatcttg aagctgtagt cgccgtcgcg cgccgcggcc
     3061 gggtcgttgt ccgcgctcat cagcaccatc gcgtccttcg gcgcggtcac gtgcgcgctg
     3121 taggtgaagc ggatgctcgg cgtgtcctgc agcggcaccc acgaacgcgc gtggatctgc
     3181 tgcgactggc tgaacatgaa gggcgtcttc ttgccctcgg tcatcgacgg ctccagccac
     3241 tgcaggccgg aggcctcggg cgaggtcgaa taggtcaccc gcacctgggt gggatgctcc
     3301 ggtgcctcga tcgtcagctt gctgcccagg atcggatcct cgtcggccag cgcgaacttc
     3361 agttcgctcc actggccttc ggcgctcagc ccctcggcct tgcggatgct gatgtcgcgg
     3421 gtatccagca ccagctgggc ggcgtccttg tccagccacg ccaggctgta ggtcgcggtg
     3481 ccagtcagcg tcttggtgcc aaagtccacc gccaggtcca gcgccaggtc ggtggtgcgc
     3541 accttgtccg gctccgcgta ggaatgttca tcggtgatcg tctcggccac cgccgcgggc
     3601 tgcgcggccg gcgtcgccga cgaaggttcc gggcctgggc tgcgcgcgca accggtggcg
     3661 acgagtccaa gggcggcggc gatcgaggcg gacagcagca actggcgcat gggcacgaac
     3721 ctgcaggcgg aaaacgccaa gtctagcgcc gtggccgaca acacgccgca tgcgcccgtg
     3781 catgaccttg gtcaacccca cttgcagcac tgcaaccttt tttcgcgaac gcgcatcgtt
     3841 tacgaaaccc tcgcttccaa cgaccccacg catgcacctt ccccgccgcg gcgccctcgc
     3901 cctcgccctc gcttgcctga tcgccccgtg cgcgccggcc ttttcgcaga cacccgccgc
     3961 ggcgactgcc ggcttcgtgc cgggcgggcg catcccgcgg ctgcaggacg ggatcgtcat
     4021 cgatggcgtg ctcgacgacg ccgcgtgggc cggcgcgctg gtgcaggaga tcggctacga
     4081 catccagccc ggcgacaaca ccccggcacc ggtgaagacc accgtccgca tcggctacac
     4141 caccgacgcg ctctacctga gctaccgcgc gctggacccg gacccggcct cgatccgcgc
     4201 ccacctgcgc gaccgcgact cggcgttcaa cgacgactgg gtcggggtgt tcatggacac
     4261 cttcgacgac cagcgccgcg gttacgaatt catcgtcaac ccgctgggcg tgcaggcgga
     4321 cctgatcaac gacgccacca ccggcaacga ggatcccagc tgggacggcc tgtggagcag
     4381 cgccggccgg atcaccgcgg atggctacga agtggagatc cggatcccgt tctccaccct
     4441 gcgcttccgc gacggcggcg gcgacaagcg ctggggcatc tcgctgttcc gcaactggcc
     4501 gcgcgacaag cgccaccagc tgaccagcca cagggtgccg cgcgaatcca actgcttcct
     4561 gtgcgaatgg acgaagtacg acggcatggc cggcgtccag cagggccgca acatcgaagt
     4621 ggtgcccacc ctgaccatgg gcgcgtcgca gtcacgcgac gctgccggca ccccctgggc
     4681 aagcggggac aactcgatcg aaccgggcct cgacgtgagc tgggcgccct cgcccgacat
     4741 gaccctcaac gccaccctca acccggactt ctcgcaggtc gagaccgacc agctgcagct
     4801 ggacatcaac aacagcttcg ccctgttcta ccaggagaag cggccgttct tcctggaagg
     4861 cgcggactac ttcaccaccc agttcgacgt gctctacacc cgccaggtcg ccgacccgga
     4921 tttcggcctg cgcaccaccg ggcgcaccgg cagcggcgcc tacggcgcga tcgtggcgcg
     4981 cgacgccagc accctggtgc tggtgcccgg ggtgctgggc tcgcgcttcg tgcagctgga
     5041 ccagccggcc aacgtggcgg tcggccgcta tcggcacgac ctcgacaggc acaccagcat
     5101 cggcgtgatc ggcaccttcc gcgaaggcga tggctacgcc aacaacgtcg ccggcgtgga
     5161 tgcgcgctgg cagcagggcg cgcacaccgc ctccgcgcaa tggctgcaca gccagtccga
     5221 gtacccggag caggtggtcg acgtctacaa gggcgaactc ggcgacgatc ccacccccgc
     5281 cggcaacgcc tggcgcgccc agtacagctt caacaaccgc aactggaacg ccagcgtgca
     5341 gcgctgggtg atcgatcccg gcttccgcgc cgacctcggc ttcatgggcc aggtcggcta
     5401 cgacaagtcg gtggccggcg gcggctacag ctggttccgc gatggcaagg cggtcaaccg
     5461 catcaacctg tacgccgact gggacatcac ccaccgcttc gacggccagc tgctggaacg
     5521 cgagctggaa ttcagcctcg gcttccaggg cccgaagcag agccagttcg gcatcaatcc
     5581 gctgggccgc gtgcgctact ggcgcggggc gatgttcaac gagcacaacc tcaacctgta
     5641 cggcaatttc cggccaatgg ccgacctgca gcttggcacc tacatgaact tcggccagca
     5701 gctggacctg cgcgccgccc gcaccggccg ccggcagatg ctcggcctgt ggggcaacgc
     5761 caatgccggg cgcggcctga ccatggactg ggacatcagc cgccaacgcc tgcgccgcga
     5821 tggcggcatc gcgttcgaag ccagcctggt caacgcgggc ggcagctggc agttcgaccc
     5881 gcgccagcgc atccgcctga ccctgcaggg cagcgaggtg ctgcgcgacc agtcgctcta
     5941 cggcacccac gtcaacgaag ccgcgcgcga ctgggccgcg caggcggtct attcgtacaa
     6001 ggtcaacccg cgcaccgcgg tgtacgcggg cgcgtcctac ggcgcgttca tggacgacga
     6061 caaccaggac ctgttcggcg ccacccgcag cgtgttcgtg aagctcagct acggctggca
     6121 gccctgaccc gcgtcagatc acgccgcgct tcttctcgaa cagcaggtag ccggccgcga
     6181 ccaccgcgcc ggccagcagc gacggcggcc agccgaacgg gctgagccac ttcaccagcc
     6241 cggccacgac cagcccgagg accacgaact gcgaatagcg ccagcggctg tcgcgaaagg
     6301 tcttcagcgg aggcctggac atcggctcag gccttgtagc cggtatggat ggcgacgatg
     6361 ccggcgttga ggttgcggta gtcgcaacgg gcgaagccgg ccgcttccat catcgccttc
     6421 agctcagcct gcggcggatg cttgcggatc gactcggcga ggtactggta actgccggcg
     6481 tcaccggcga acagcttgcc cagccgcggc agcacctgga acgaatggaa gtcgtagatc
     6541 ggcctgaacc actccgcctg cacttcggag aattccagca cccgcgcctg cccgccgggc
     6601 ctgagcacgc gcagcatctc gcgcagcgcg gcgtccttgt cggtgacgtt gcgcaggccg
     6661 aacgcgatgg tgaccaggtc gaagcttgcg tccgggaacg gcagcgactc ggcgttgcac
     6721 tgcacgtact cgaacccacg cacgttgccg cggtcggtca tgcggtcgcg accgacgcgc
     6781 agcatctcgc cgttgatgtc gccgagcacc agttccccgc cttcgccgac gcgctccttc
     6841 agcagggcgg cgatgtcgcc ggtgccgccg gcgaggtcga gcacgcggtc gcccttgcgc
     6901 acctgcgcgg tggccacgaa gtagcgcttc cacagccggt ggatgcccag gctcatggcg
     6961 tcgttcatca ggtcgtagtt gccggccacc gaggagaaca cctcgccgac cagcttctgc
     7021 ttctcggcca ccgggacgtc gcggaacccg aagtgggtgg tgccgggctt gcctgtcgat
     7081 tcgctcatcc cggcattatc gcaccggccc gactgatcgc gatcaaggca ggccggcgga
     7141 cacgcgcgca gaatgacgga aacacctgga gccgcccatg ccggaccatc ccgcccgcgc
     7201 cgccgtcctg cctgccctga tcgcggacga cgccgccctc gaccgctacc tcggcgagtt
     7261 ccagcgcacc gcctatgcca tgcagcgcac ccaggccggg atcagcatgg ccgactggct
     7321 ggccaccggc gaagacgcct gcgagcgctg ctggctgcat gcgcagcggc tcgacgtgcc
     7381 gctggtgcgg ctgccccagg ttagcgccga tcccgccacc gcctgcctgc tgcggccgga
     7441 acaggcgcgc cgcctgcgcg tggtgccgct gcgcgccgcg catggactgc tggcggtggc
     7501 gatggaggac ccgtccgaca gcgacgcgct ggccgcggtc gagttcctca gcggcaaccg
     7561 cgtgctgccg ctgatggcca ccgcgcgcgg catccgcgag gcgatcgcgc gcagctacga
     7621 ccaggtcgag gaccgcgaaa ccgtgcgcct gctcggcctg gaccccggcg ccagcgtcga
     7681 caccagcgag tccgaggccc tgcgcctgtc gcgcgagcag ccggtggtgc ggatcgtgca
     7741 cgggctgatc gccgatgcag tggcccggcg cgcctccgac atccacctgc gtcccggcga
     7801 acgtggcacc gacgtgctgt tccggatcga cgacgaactg gtgccggtgc gcagcctgct
     7861 gcgcgccctg cacgcggcgg tggtgagccg gatcaaggtg ctcggcggga tgaacctggc
     7921 cgagcaccgg cgcccgcagg atggccgcac cagcttcacc ctcgacgacg gcaccagggt
     7981 cgacctgcgc gtctcggtgc tgccggcggt gttcggcgaa agcgtggttg tgcgcctgct
     8041 ggacacccgc gagagcctgt ggaacctgga gcagctgggc ctcgacgaca ccgaccgccg
     8101 ccgcatcgac gacgtgatga gccgcagcca cggcatgttc ctggccaccg gcccgaccgg
     8161 ttgcggcaag tccaccaccc tgtacgcgat gctgatggag ctgcgccggc agcgcatcaa
     8221 catcctcacc gtcgaggatc cggtcgaatt ccacatcgcc gagatccagc agatgcaggt
     8281 caaccgcgcg gccggcttca ccttcgccag cgccatgcgc aacttcctgc gccacgaccc
     8341 ggacgtgatc atggtcggcg agatccgcga ccgcgagacc gccgacatcg cggtggaaag
     8401 cgcgctgacc gggcacctgc tgctgagcac gctgcacacc aacaccgccg ccaccaccat
     8461 cacccgcctg ctcgacctcg gggtggaggc ctacctgctg cgcgccagcc tgctggcggt
     8521 gatctcgcaa cggctggtgc ggctgacctg cccgcactgc cgcgaggtcg agaccgtgga
     8581 tccccacgtg cgtgccgcgc tcggcgtcgg cgccgacgag gtcttccacc ggggccacgg
     8641 ctgcagccag tgcgagggcc tcggtgtgta caagcggcag gcggtgtacg agttcctggt
     8701 gatgagcccg cgcctgcgcg aactggtcgt gcccggtgcc gaagcggaca ccatccaccg
     8761 ggtcgcggtc gaggagggca tgcgcccgat cacccaggcc gcggtcgcac tggcgcgcag
     8821 cggcgcgatc tcgctggcgg aggcatggcg ggtgcgcgcc gactgaggcc gcggcggcga
     8881 tcggatcgcg ttccattgca gatgcgaacg ccacccaacc ggatccacct cgtgctgcgc
     8941 atcgacggcg aggccgtccc ggtctccgcg ccacgacctc cgttgcgcgc acgcctggac
     9001 gaactgctgc cggcgcagcg cgcggtcgac aacgccgcca tcgaccatgc cgtgcgcaag
     9061 gccgcggccg ccggcaagca ggtgacctgc gcgaagggct gctccgcctg ctgccgcgcg
     9121 cagccggtgc cggtgacgcc ggccgaggcc tacgccttgt tgcggctggt ggaagcgttg
     9181 cccgcggcgc ggcgcgaaca ggtcgaagca cgcttcgacg accgcgtgca acgcctgcgc
     9241 ggggccggcc tcgccgacct gctgctggac cgcgccgatg acctcgacgc cgaacgcgcg
     9301 cgcggtctcg cccttgccta cttcaagctc gggctggtgt gtccgttcct ggccgaggac
     9361 gcctgcagca tccatccgca gcgcccgttc gtgtgccgcc aatacctggt cagctccgac
     9421 ccggcactgt gcgcggaccc gttcgcgaac cacgccgagg tcatcccgat gccgctgcac
     9481 gccgccaccg cggcactggc gatcagcgag accgcctatg gccgcgcgca gcacagcgtg
     9541 ccgctgacgc tcgcgctgga gtacgcgcgc aggcatcgcg cggaactgga acggcagttc
     9601 gacgcggagc cgttgttccg gcagtggctg ggggcgctgt agcgccccgg gccgcaagcg
     9661 atcaggcggc ggtggcctgc gccctggcca gggtggcgat caggtcgcgg atcgccttgc
     9721 gctgcgcgct gggcagggcc aggaatgcat cgatggccaa ctggtcggcg gccgtgactt
     9781 tccaggcctt cccgggctcc cggatatttc gaccatcagg atcgccgtat tgcagcgccc
     9841 gcgggtcgat ccgcagcatc ttcgccaatg caacgaggtt ggcttgccgg ggcacgctct
     9901 tgggcggggg gatcaccatc gaagccgaac tcggctgagg cagcgcgtgc ctcagcggta
     9961 atcgtcttcc cg
//
LOCUS       DAORUK010000072         9719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_7451_length_9719_cov_18.522765, whole genome shotgun sequence.
ACCESSION   DAORUK010000072 DAORUK010000000
VERSION     DAORUK010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 9719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9719
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7451_length_9719_cov_18.522765"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1906)
                     /locus_tag="PK818_06280"
     CDS             complement(<1..1906)
                     /locus_tag="PK818_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY82375.1"
                     /translation="MALNLRALIARLNTTVRGAMEGAAGLCMSRGHYEVEVEHVLLKL
                     MEVENTDIRRILRQFEIAPEALERELGKALDGFKAGNQRTPALSAMIPKLFEAAWGWA
                     SIEYGEAKIRSGHVLVALLADSELRRLVSVSARSLEKINFETLTKNFHALTEASSEAR
                     DARALGDAGGALSGTGADAATPGAAAAGGKPSRTPNLDQYCINLTQRAREGKLDPVLG
                     RDAEIRLMIDVLTRRRQNNPILTGEAGVGKTAVVEGLAARIATDDVPPILRGVELLSL
                     DLGLLQAGASVKGEFENRLKGVIEEVKSSPKPIIMFIDEAHTLIGAGGAAGQNDAANL
                     LKPALARGEMRTIAATTWSEYKKYFEKDPALTRRFQVVKVDEPDDAKAVSMLRGVGQA
                     MAAHHKVRILDEAITEAVKLSSRFIPGRQLPDKAVSVLDTACAKIAIGQNATPARIED
                     ARRRLDVLATERAQLEKEAAGGSDHGKRLAEIAEETAGASETLAADEARFAQEKELVA
                     KIHAELDKVEALPADDPGRADAVATLNAIRAELKALQGFEPMILPVVDSSAVAEIIAG
                     WTGIPVGRMVADEIQTVLRLQDTLEERVIGQSHALEAIAQRVRTSRANLEDPDKPKGV
                     FLLVGTSGVGK"
     gene            complement(1897..2997)
                     /gene="tssG"
                     /locus_tag="PK818_06285"
     CDS             complement(1897..2997)
                     /gene="tssG"
                     /locus_tag="PK818_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HQY82376.1"
                     /translation="MNLGTPIDGATASPLVAPGDPMQPAIDRLLNDPGRFGFFQAVRL
                     LYSVGGFDGRGTGARPGPLRFTTPASLSFPPSELHSIAQADASTRVCVNFFGLTGPSG
                     VLPRTYTELLIARKQVQRDQSAQEFFDLFNHRLVSLFWLAWAKHRPEIGRQFGFNNSV
                     YRYLEHIVGLGTPALQARLHPTRRGATPVKALPSAALSYFSGLIAQRPHGERAIAQVV
                     SAVVAAPVQATGCLGTWQDIAADARTRLGRGNHRLGAGCVLGTRYWDRQTTLRLTIGP
                     IDRARFNALLPRGARLADIIELTRFLTGLALDLRIRLSLRADQVPPLRLGARDQDRPQ
                     LGWNTWLRGRSDPRPANDCEFHFSALGGQSWH"
     gene            complement(3022..4872)
                     /gene="tssF"
                     /locus_tag="PK818_06290"
     CDS             complement(3022..4872)
                     /gene="tssF"
                     /locus_tag="PK818_06290"
                     /inference="COORDINATES: protein motif:HMM:NF017733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HQY82377.1"
                     /translation="MPGNAMLDELLPYYENELTYLRRLSKEFASKYPKIASRLQMEGE
                     VCEDPHVERMIESFAFLTSRIHKKLDDEFPQITEAMLSVLYPHFLRPVPSMSVAQLQL
                     APGAEISAMQELPRHSQLLTRPVQGLPVRYRTGYPVEVWPIRIAAAQLEAAERSAFAV
                     TQDDTVATIRIRVEATGQLPLSKLPIRRLRLYIDGESPLVHALHELLLNSATGITLRA
                     PDDAPQVPPLRLPATCLQPVGFADEDALLDYDPRSFLGYRLIQEYFVLPEKFLFIDLA
                     GIDTSRFDRAMEVAIELGSFGRPERLQRLEQAVSADTFRLFCTPIVNLFKQQGEPIRV
                     SQERHEYQVVPDVRRPLGLEVYSVDWVRKASRTPTSNDVVEFHPAYSVKHGLHDEGDG
                     HYWYLQRRPSLLPNDDGSDVMISLVDRDMNPRAPAVDTLSLSLTCTNRDLPSALPFGG
                     DDSQLQLDQGGVIASARLLKKPSATWRAPMRLANQWRLISHLSLNHLSIVDGGREALL
                     EILSLYNFADSVTVRKQIAGISNVSGHPSVTRVGQAPRQAFVRGTEIELEFDENQYVG
                     SGVYLMACVLDRFLGLYCTANSYTRLSVRSQQREDYIVRFAPRSGSLPLV"
     gene            complement(4978..6843)
                     /locus_tag="PK818_06295"
     CDS             complement(4978..6843)
                     /locus_tag="PK818_06295"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HQY82378.1"
                     /translation="MNRTAPVSRHRPLRLPWLALGIAISSFAAAQSAPDPAAAPTNPD
                     LGPAFSTKNAAYVPVLFDEIPGWEDESFEQTYASFSSNCKAMKRRSAWAPICAKLARL
                     PKNDAALRAFMHDEFYAYQMLTPTRGATGKLTGYFEPLIAGSLRSEGAYRYPVYGIPN
                     DMYMVDSRTVAGQSSRWLKPAQGKLVGAEAGTPGARQYKIDLAGTSPNNRDKRYRVRI
                     VGDSVRPYLTRKQIDAGGIDAPVLAWVADPYKLYSMQIQGSGKIQLKDGGLIRLAYAE
                     QNGYPFMPNATRGDQDNMLLAIKARALMEPPTVDVPGATRGAKKSGGSSASDDEVARI
                     VAALSSGGSTTASTSRPKAKAKPRPVAKKAASKRPAQGTPGEAQEEIAVDPSDVDAMI
                     QFLLSAPVADESVYVADAEAAPEPATATSSPRPSSAQSSAQKGAPMDMSEFEGKGGGK
                     GAGQAVGSGKAVPATGIHDPSYVFFRRIGDGPEGPIGALGVPLSAGRSMAVDPRATPL
                     GAPVFIDGQQPGGKGPMRRLVFAQDTGGAIRGSVRGDFFWGFGDKAGKMALQTNQPVK
                     MWLLLPKAVAGSAAASGIKTRSAGGVEALRDCAVPDEEFCVEDDGSILDELLPGG"
     gene            complement(6950..7618)
                     /locus_tag="PK818_06300"
     CDS             complement(6950..7618)
                     /locus_tag="PK818_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQY82379.1"
                     /translation="MKVLIADDHRLIIEGVKIKLAELDPALEAVVAMNLEELDRAVAA
                     HAHELDLALVDIAMPGTHGFEHVARLRSQAPALPVIVLSGSEDVELMRALIDLGVLGF
                     IPKAYSPEVMLSAIRLVLAGGIYVPPLLLANAQAQGWQPSEAPAAEGAPSRSIDGLRN
                     LLTERQIDVMRLLSQGKPNKLIARDLGISEGTVKIHLAAIFRALNVRNRVEAVVASRK
                     LQGL"
     gene            7827..9080
                     /locus_tag="PK818_06305"
     CDS             7827..9080
                     /locus_tag="PK818_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HQY82380.1"
                     /translation="MTHAARQRAVQTHARSSACGLLPAMLLSAAVSAGVPPQDAPSAA
                     QASQPPQPQLQDAQTRGVSAQAATDAVASQLPPTDPVSQSSAAPQALAAGDAVPASIA
                     PRLPVAPVLRGVLFRVTPPAATLLPAEALQAQGDSPAPPATASAGDPAPMPTPAPAAP
                     APMRASYLLATIHFGTPEEQGIDYAQLQQTLAEVDTFVNEANLDEAWKPELDGYRWLP
                     EQSPLSNLVGKDGFAQARALLPNVRAQDLDRMKPWSVLALLEARGETGGDATMDARLQ
                     RMAAGAGKRVLHLETLEQQLQALDCVPAEEHSRVLDERLRASWVLRIESAQAMAWYRA
                     RNLDAWLADIDRMQGLSDEARAIEQRARRCLLEDRNARWIGQLETLFQDGPCLVAVGA
                     VHLAGAEGLLAALRRDGYLVEAMPL"
     gene            9172..>9719
                     /gene="tssK"
                     /locus_tag="PK818_06310"
     CDS             9172..>9719
                     /gene="tssK"
                     /locus_tag="PK818_06310"
                     /inference="COORDINATES: protein motif:HMM:NF017723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HQY82381.1"
                     /translation="MSGDRQVHWGQGAFLSPQHLQAQDAWHQAGRHSLHLRLTPFPWG
                     YEALRLREDALGSGVIDIERFALVTRDGERLVGGSLDTAGGNTRVPQRNIAELATGSN
                     DPIPAWLVFNRERAIQGLATRESERLLARHALATDSLADPFDPQAPAADVDFLLYQPN
                     IVTGLDENAEVIVRSSDSYKFA"
BASE COUNT         1376 a         3358 c         3462 g         1523 t
ORIGIN      
        1 tcttgccgac gccggaggtg ccgaccagca ggaacacgcc cttcggcttg tccgggtcct
       61 ccaggttggc gcgcgaggtg cgcacgcgct gggcgatcgc ctcgagcgcg tggctctggc
      121 cgatcacgcg ctcctcaagc gtgtcctgca ggcgcagcac ggtctggatc tcgtcggcga
      181 ccatccggcc gaccggaatg ccggtccagc cggcgatgat ctcggcgacc gcgctggaat
      241 ccaccaccgg caggatcatc ggctcgaagc cctgcagtgc cttcagctcg gcgcggatgg
      301 cgttgagcgt ggccaccgcg tcggcgcgac cgggatcatc ggccggcaat gcctcgacct
      361 tgtcgagctc ggcgtggatc ttcgccacca gttccttctc ctgcgcgaag cgggcctcgt
      421 ccgcggccag ggtttcgctg gcccccgcgg tctcctcggc gatctcggcg aggcgcttgc
      481 catggtcgct gccgccggcg gcttcctttt ccagttgcgc acgctcggtg gccagcacgt
      541 ccagccgcct gcgcgcgtcc tcgatccgcg ccggggtggc gttctggccg atcgcgatct
      601 tggcgcaggc ggtgtcgagc acgctgaccg ccttgtccgg cagctggcgg cccgggatga
      661 agcgcgagct cagcttcacc gcttcggtga tcgcctcgtc gaggatgcgc accttgtggt
      721 gcgcggccat cgcctggccg accccacgca gcatcgacac cgccttggca tcgtcgggct
      781 cgtcgacctt gaccacctgg aagcgacggg tgagggcggg gtccttctcg aagtacttct
      841 tgtattcgct ccaggtggtt gcggcgatgg tgcgcatctc gccgcgcgcc agggcgggtt
      901 tcagcaggtt ggccgcatcg ttctggccgg ccgcgccgcc ggcgccgatc agggtgtgcg
      961 cctcgtcgat gaacatgatg atcggcttgg ggctggactt gacctcctcg atcacgccct
     1021 tcagccggtt ctcgaattcg cccttgacgc tggcgccggc ctgcagcagg ccgaggtcca
     1081 gcgacagcag ttcgaccccg cgcaggatcg gcggcacgtc gtcggtggcg atccgcgcgg
     1141 ccagtccctc gaccaccgcg gtcttgccca cgccggcctc gccggtgagg atcgggttgt
     1201 tctgccggcg ccgggtcagc acgtcgatca tcaggcggat ctcggcatcg cgcccgagca
     1261 ccgggtccag cttgccctcg cgcgcgcgct gggtcaggtt gatgcagtac tggtccaggt
     1321 tcggcgtgcg gctgggcttg ccgccggctg cggccgcgcc cggggttgcc gcgtcggcgc
     1381 cggtgcccga cagggcgccg ccggcgtcac ccagggcgcg cgcatcgcgc gcttcgctgg
     1441 aggcctcggt gagcgcatgg aaattcttgg tcagggtctc gaaattgatc ttctcgagcg
     1501 aacgcgcgct gacgctgacc agccggcgca gttccgaatc cgccagcagc gcgaccagca
     1561 cgtggccgct gcggatcttg gcttcgccgt attcgatcga cgcccagccc catgcggcct
     1621 cgaacagctt cgggatcatc gccgacaacg ccggggtgcg ctggttgccg gccttgaagc
     1681 cgtcgagcgc cttgcccagc tcgcgttcca gcgcctccgg cgcgatctcg aactggcgca
     1741 ggatgcggcg gatgtcggtg ttctccacct ccatcagctt cagcagcacg tgctcgacct
     1801 cgacctcgta gtggccgcgc gacatgcaca ggcccgccgc gccttccatc gcgccgcgga
     1861 cggtggtgtt caggcgtgcg atcagcgcgc gcaggttcag tgccatgatt ggcctccaag
     1921 ggcggaaaag tggaattcgc agtcgttcgc gggacgcgga tcgctgcgcc cgcgcagcca
     1981 ggtgttccag cccagttgcg ggcggtcctg gtcgcgtgcg ccaagccgca gcggcgggac
     2041 ctggtcggcg cgcagcgaca ggcggatgcg caggtccagc gccagtccgg tcaggaagcg
     2101 ggtcagttcg atgatgtccg ccagcctggc gccgcgtggc agcagggcgt tgaacctggc
     2161 gcggtcgatc ggtccgatgg tcaggcgcag ggtggtctgc cggtcccagt agcgggtgcc
     2221 gagcacgcag ccggcgccga ggcggtggtt gccgcggccg agccgggtgc gcgcatcggc
     2281 ggcgatgtcc tgccaggtgc ccaggcagcc ggtcgcctgc accggcgccg cgaccaccgc
     2341 cgacaccacc tgcgcgatcg cgcgttcgcc gtgggggcgc tgcgcgatca ggccggagaa
     2401 gtagctcagg gccgcgctgg gcagcgcctt caccggcgtg gcgccgcgcc tggtcggatg
     2461 cagccgcgcc tgcagcgccg gcgtgccaag gccgacgatg tgctccaggt aacggtagac
     2521 cgaattgttg aaaccgaact ggcggccgat ttcgggccgg tgcttggccc atgccagcca
     2581 gaacagcgac accagccggt ggttgaacag gtcgaagaat tcctgcgcgc tctggtcgcg
     2641 ctgcacctgc ttgcgcgcga tcagcagctc cgtgtaggtg cgcggcagca ctccggaggg
     2701 cccggtgagg ccgaagaagt tgacgcacac gcgggtgctg gcatccgcct gcgcgatcga
     2761 atgcagctcc gatggcggga acgacagcga ggccggggtg gtgaagcgca gcgggccggg
     2821 gcgggcgccg gtgccgcggc cgtcgaagcc gccgacgctg tacagcagcc gcaccgcctg
     2881 gaagaagccg aagcggccgg gatcgttgag caggcggtcg atcgccggct gcatcgggtc
     2941 gccgggcgcg accagcgggc tggcggtggc gccgtcgatg ggggtgccca ggttcatgcg
     3001 gcgctgttca tcggcagggg ctcataccag cggcaggctg ccgctgcgcg gggcgaagcg
     3061 cacgatgtag tcctcgcgct gctggctgcg cacgctcagg cgggtgtagg agttggcggt
     3121 gcagtacagg ccgaggaagc gatccagcac gcaggccatc aggtacacgc cggacccgac
     3181 gtactggttc tcgtcgaact ccagttcgat ctcggtgccg cgcacgaagg cctggcgcgg
     3241 cgcctgcccg acccgggtca ccgacggatg gcccgacacg ttgctgatcc cggcgatctg
     3301 cttgcgcacc gtgaccgagt ccgcgaagtt gtagaggctg aggatctcca gcagggcctc
     3361 gcggccgccg tcgacgatcg acaggtggtt gagcgacagg tgcgaaatca gccgccactg
     3421 gttggccagc cgcatcggcg cgcgccaggt cgcgctgggc ttcttcagca gccgcgcgct
     3481 ggcgatgacg ccgccctggt cgagctgcag ctggctgtcg tcgccgccga atggcagcgc
     3541 cgagggcagg tcgcggttgg tgcaggtcag gctcagcgac agcgtgtcca ccgccggtgc
     3601 gcgcggattc atgtcgcggt cgaccaggct gatcatgacg tcggagccgt cgtcgttcgg
     3661 cagcagcgac ggtcgccgct gcaggtacca gtaatggccg tcgccttcgt cgtgcaggcc
     3721 gtgcttcacc gaataggcgg gatggaactc gaccacgtcg ttgctggtcg gggtccggct
     3781 ggccttgcgc acccagtcca ccgagtacac ctcgagcccg agcgggcggc gcacgtccgg
     3841 caccacctgg tactcgtggc gctcctgcga gacccggatc ggctcgccct gctgcttgaa
     3901 caggttgacg atcggcgtgc agaacaggcg gaaggtgtcc gccgacaccg cctgttccag
     3961 ccgctgcagg cgttcggggc ggccgaagct gccgagctcg atcgccacct ccatcgcgcg
     4021 gtcgaagcgg ctggtgtcga tcccggccag gtcgatgaac aggaacttct ccggcagcac
     4081 gaagtattcc tggatcagcc ggtagccgag gaaggaacgc gggtcgtagt cgagcagggc
     4141 atcctcgtcc gcgaacccga ccggctgcag gcacgttgcc ggcagccgca gcggcggcac
     4201 ctgcggtgca tcgtccggcg cgcgcagggt gatgccggtg gcggaattga gcagcagttc
     4261 gtgcagcgcg tgcaccagcg ggctctcgcc gtcgatgtac aggcgcaggc gccggatcgg
     4321 cagcttcgac agcggcagct gccctgtcgc ttcgacccgg atccggatgg tggcgacggt
     4381 gtcgtcctgg gtgaccgcga acgccgaccg ctccgcggcc tccagctgcg ccgcggcgat
     4441 ccggatcggc cagacctcga ccgggtagcc ggtgcggtag cggaccggca ggccctgcac
     4501 cggccgcgtc agcagctggc tgtggcgcgg cagctcctgc atcgcgctga tctcggcgcc
     4561 gggcgccagc tgcagttgcg ccaccgacat cgacggcacc gggcgcagga aatgcgggta
     4621 cagcaccgac agcatcgctt cggtgatctg cgggaattcg tcgtcgagct tcttgtggat
     4681 gcgcgaggtc aggaacgcga acgactcgat catccgctcg acgtgcggat cctcgcagac
     4741 ctcgccttcc atctgcaggc gcgaggcgat cttcgggtac ttggaggcga actccttcga
     4801 cagccgtcgc aggtaggtca gttcgttctc gtaatagggc agcagctcgt cgagcatggc
     4861 gttcccgggc atggaccggg cgtcgcggcg cgcgcgcccg gtatcgccac aggctagata
     4921 gccgccgccg gtgcggccat tctccggatg gcctatgccg tgtgccggcc cgcgccgcta
     4981 gccgccgggc agcagctcat cgaggatgct gccgtcgtcc tccacgcaga actcctcgtc
     5041 cggcaccgcg cagtcgcgca gtgcctcgac cccgccggcg ctgcgggtct tgatcccgct
     5101 ggcggcggcg ctgccggcca ccgccttcgg cagcagcagc cacatcttga ccggttggtt
     5161 ggtctgcagc gccatcttgc cggccttgtc gccgaagccc cagaagaagt cgccgcgcac
     5221 gctgccgcgg atcgcgccac cggtgtcctg ggcgaacacc agccggcgca tcggtccctt
     5281 gccgccgggt tgctggccat cgatgaacac cggtgcgccg agcggggtgg cgcgcgggtc
     5341 caccgccatc gaacggcccg cgctcaaggg cacgccgagc gcgccgatcg ggccttccgg
     5401 gccatcaccg atgcggcgga agaacacata gctcgggtcg tggatgccgg tggccggcac
     5461 cgccttgccg ctgcccaccg cctggcccgc gcccttgccg ccgcccttgc cctcgaactc
     5521 cgacatgtcc atcggggccc ccttctgggc ggaggactgc gcggaggacg gccgcggcga
     5581 gctggtcgcg gtggccggtt ccggcgcggc ttccgcgtcc gcgacataga cgctctcatc
     5641 ggcgaccggt gcggacagca ggaactggat catcgcgtcc acgtcggacg ggtctaccgc
     5701 gatctcttcc tgcgcttcgc ctggcgtacc ctgggcgggg cgcttggacg ccgctttctt
     5761 ggccaccggc ctgggcttcg cctttgcctt cggccttgag gtgctggccg tggtgctgcc
     5821 gccgctgctc agggcagcga cgatgcgcgc gacttcgtcg tccgatgcac tcgaaccgcc
     5881 ggatttcttc gcaccacggg tcgcgcccgg cacatcgacg gtgggcggct ccatcagggc
     5941 gcgcgccttg atcgccagca gcatgttgtc ctggtcgccg cgggtcgcgt tcggcatgaa
     6001 cgggtagccg ttctgctccg cgtaggccag ccggatcagg ccgccatcct tgagctggat
     6061 cttgcccgag ccctggatct gcatcgaata cagcttgtac gggtcggcca cccacgccag
     6121 caccggcgca tcgatcccgc cggcgtcgat ctgcttgcgg gtgaggtagg ggcgcacgct
     6181 gtcgccaacg atgcgcaccc ggtagcgctt gtcgcgattg ttgggcgagg tcccggcgag
     6241 gtcgatcttg tactggcgcg cgcccggcgt gccggcctcg gcgccgacca gcttgccctg
     6301 cgcgggcttc agccagcggc tggactggcc tgccacggtc cgcgaatcga ccatgtacat
     6361 gtcgttgggg atgccgtaca ccgggtacct gtaggcgccc tcgctgcgca gcgacccggc
     6421 gatcagcggc tcgaaatagc cggtcagctt gccggtcgcg ccgcgggtcg gggtcagcat
     6481 ctggtaggcg tagaactcgt cgtgcatgaa cgcgcgcagg gcggcgtcgt tcttcggcaa
     6541 ccgggccagc ttcgcgcaga tcggcgccca cgccgagcgc cgcttcatcg ccttgcagtt
     6601 ggaggagaag ctggcgtagg tctgctcgaa gctctcgtcc tcccagcccg ggatctcgtc
     6661 gaacagcacc ggcacgtagg ccgcgttctt ggtcgagaac gcggggccga ggtcggggtt
     6721 ggtcggcgcg gcggccgggt ccggggcgct ctgggcggcg gcgaagctgc tgatcgcgat
     6781 ccccaatgca agccacggca ggcgcaacgg gcgatggcgc gaaacgggtg cggtgcggtt
     6841 catggcgagc ctccacggcc gcgtgcgggc cgtgcatacg tgctcaggct gcgcccgcgc
     6901 gaatccgcgg acaatacggc ggacgcacta tggcccgcgg actggggtgc tacaggccct
     6961 gcagcttgcg cgaagcgacc accgcctcga cccggttgcg cacgttcagc gcgcggaaga
     7021 tcgccgccag gtggatcttc accgtgccct cgctgatccc gaggtcgcgc gcgatcagct
     7081 tgttcggctt gccctgcgag agcaggcgca tcacgtcgat ctggcgttcg gtcagcaggt
     7141 tgcgcaggcc atcgatcgag cgcgagggcg cgccctccgc cgccggcgcc tcgctcggct
     7201 gccacccttg cgcctgcgca ttcgccagca gcagtggcgg gacgtagatg ccgccagcca
     7261 gcaccagccg gatcgccgag agcatgacct cgggcgaata cgccttgggg atgaagccga
     7321 gcacgccgag gtcgatcagg gcgcgcatca gttcgacgtc ctccgagccg gacagcacga
     7381 tcaccggcag cgccggtgcc tgcgaccgca gccgcgccac gtgctcgaag ccatgggtgc
     7441 cgggcatcgc gatgtcgacc agcgcgaggt cgagttcgtg cgcatgcgcg gcgaccgccc
     7501 ggtccagctc ttccaggttc atcgccacca ccgcttccag cgccggatca agctcggcga
     7561 gcttgatctt gacgccctcg atgatcaggc gatggtcgtc cgcgatgagc accttcattg
     7621 cggcaccttc atgggtggtg tctccacgcg cgatgtggcg aacgcaaccg cgctctcggg
     7681 tgttcacgat attccgcgcc accagcggtc atcaatacgg atgcgccgtt ccaacggcat
     7741 agctcctccg acatatggtt gcgcgcggcg cgctcgccaa agatgcgttg catcgcgccc
     7801 cgcgggcgct gcgaacgaat gccccaatga cccacgcggc acgccagcgc gcagtccaga
     7861 cgcacgcacg cagcagtgca tgcggcctgc tgcctgcgat gctgctgtcg gcggcggtgt
     7921 ccgccggcgt gccgccgcag gacgccccgt cggcggcaca ggcttcgcaa ccgccgcaac
     7981 cgcaactgca ggatgcgcag acccgcggcg tgtccgcgca ggcggcgacc gatgcggtgg
     8041 cctcgcaact gccgcccacg gatccggttt cgcaatcatc ggctgcgccg caggcgctgg
     8101 cggcaggcga tgccgtgccc gccagcatcg cgccacggtt gccggtcgcg cccgtgctgc
     8161 gcggcgtgct gttccgggtg accccgccgg cggcgaccct gctgccggcg gaggcgctgc
     8221 aggcgcaggg cgactcgccg gcaccgcctg cgacggcgag tgccggcgat cccgcgccaa
     8281 tgccgacgcc agcgccggca gcacccgcgc cgatgcgcgc cagctacctg ttggcgacca
     8341 tccacttcgg taccccggaa gagcagggca tcgactacgc gcagctgcag cagacgctgg
     8401 cggaggtcga caccttcgtc aacgaagcga acctggacga ggcctggaag cccgaactcg
     8461 acggctatcg ctggctgccg gaacagagcc cgctgtcgaa cctggtcggc aaggacggct
     8521 tcgcgcaggc acgcgcactg ctgccgaacg tgcgcgcgca ggacctcgac cggatgaagc
     8581 cgtggtcggt gctggcgctg ctggaggcac gcggcgaaac cggcggcgac gccaccatgg
     8641 acgcacgcct gcagcggatg gcggcaggcg ccggcaagcg cgtgctgcac ctggagaccc
     8701 tggagcagca actgcaggcg cttgactgcg tgcccgcgga ggaacattcg cgggtgctcg
     8761 acgaacgcct gcgggcatcg tgggtgctgc gcatcgagtc ggcgcaggcg atggcctggt
     8821 atcgcgcgcg caacctggat gcgtggctgg ccgacatcga ccgcatgcaa gggctgagcg
     8881 acgaggcgcg tgcgatcgag caacgcgcac gccggtgcct gctggaagac cgcaacgcac
     8941 gctggatcgg ccagctggaa acgctgttcc aggacgggcc ctgcctggtg gcggttggcg
     9001 cggtgcacct tgccggcgcc gagggcctgc tcgcggcgct gcgtcgtgat ggctacctgg
     9061 tggaggcgat gccgctatga gcaccggatt gcgttgcacc tggggtgggc cacgtacaca
     9121 cggcaacagc ggcgggcaag ccatgcccgc ggcatcggtt ggagcgacgg catgagtggt
     9181 gatcgacaag tccattgggg gcagggcgcg ttcctgagtc cgcagcacct gcaggcgcag
     9241 gacgcgtggc atcaggccgg ccggcattcg ctgcacctgc ggctgacgcc gttcccatgg
     9301 ggctacgagg cgctgcgcct gcgcgaggat gcgctcggca gcggggtgat cgacatcgag
     9361 cggttcgcgc tggtgacccg cgacggcgag cgcctggtcg gcggatcgct ggacaccgcc
     9421 ggcggcaaca cccgcgtgcc ccagcgcaac atcgccgagc tggcgacggg cagcaacgac
     9481 ccgatcccgg cgtggctggt gttcaaccgc gagcgcgcga tccagggcct ggccacgcgc
     9541 gaatccgaac ggctgctggc gcggcacgcg ctggccaccg acagcctggc cgatccgttc
     9601 gatccgcagg cgccggcggc cgacgtcgac ttcctgctgt accagccgaa catcgtcacc
     9661 gggctggacg agaacgccga ggtgatcgtg cgctcctccg acagctacaa gttcgccga
//
LOCUS       DAORUK010000073         9462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_7782_length_9462_cov_18.600404, whole genome shotgun sequence.
ACCESSION   DAORUK010000073 DAORUK010000000
VERSION     DAORUK010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9462
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7782_length_9462_cov_18.600404"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="PK818_06315"
     CDS             <1..881
                     /locus_tag="PK818_06315"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082741.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HQY82382.1"
                     /translation="LPNAGIGLARLEMIIASHIGVHPNALLDYARQDAATKARIEART
                     AGYAGPVEFYVDRLAEGIATITASVAPNPVIVRMSDFKSNEYANLIGGGKYEPHEENP
                     MIGFRGASRYIDPSFKQAFALECRAVKRVREDMGLDNLWVMIPFVRTVDEGRKVLEVL
                     AENGLRRGEGGLRVIMMCEVPSNALLADEFLDLFDGFSIGSNDLTQLTLGLDRDSAIV
                     AGLFDERDPAVKKLLAMAISAAKRRGKYIGICGQGPSDHPDLALWLMEQGIESVSLNP
                     DTVVDTWLALAKARAG"
     gene            1072..1920
                     /locus_tag="PK818_06320"
                     /pseudo
     CDS             1072..1920
                     /locus_tag="PK818_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157560.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
     gene            complement(1930..4866)
                     /locus_tag="PK818_06325"
     CDS             complement(1930..4866)
                     /locus_tag="PK818_06325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQY82383.1"
                     /translation="MLALLLCVGALPAIARAQDKPLTAYFRETWTTRQGLPHNQVNAI
                     AQTPDGYLWFGTWEGLVRYNGLEFHSFDRGNTPELKDNGVRSVRASADGALVIGTSRG
                     GVTVKRGDHWRTWRVKDGLAQEEIMDAIFDRRGRLWVATENAGIDVVDGSRVDHFTTR
                     NGLPSAVTYGLLLDRDGSVWAATARGLVHFLDGRLVVYAQASGLPPAPVFRVFQDAGG
                     RLFVGTERGVYRRQGERFVLASALLPDDGVPSMAQDGAGNLWVGSVNNGLLRLGKSGV
                     ETFTSLRGLPNNRVAALLVDREGSIWAGTNAGLLRLSDAPFSTYNGDHGLSDDYVRAV
                     LESRDGSLWLGTSRGLNRWRGGRLEASYTAADGLPSDSVLSLLEDRDGSLLVGSYTAG
                     VMRLRDGKLVAHYDNAHGMPGSNQVRALAQQADGTLWLGTNRGLVRMRDGAYTHFGVA
                     QGLPREFIISLQVARDGSLWVGTANGAARIVDGRVQALDMRGMNGAQDVFDFHEDADG
                     TLWFATDRGLLRYRGGRLQALGLAQGLPVDTLFAVVDDGIGSLWLTSNRGVMRVGRSE
                     VEAVMDGRKPRLELDRFGEADGLASYQCNGGSGPAALRDRGGRIWIATAGGAAVVEPK
                     ALRAYKRTLPPVLVEQVLANDRDVQVQGRLELPAGTRKLEFHYASLTFQTPRFVRYRY
                     QLQGVDRDWIERGNQRVAQYTNVAPGNYRFRVNASAPGLGHGWSPDVTTLEIGIAPRL
                     WQRPWFLPLLLLFGVLALAGLYRWRLGHMRQRAADLEAVVAARTRDLREQTERLRESD
                     REKSFLVDKLREQSEAFERQAREDALTGLGNRRSMDEELARAFEQAVQSGRPLSFALI
                     DIDHFKRINDGYSHAAGDRALVEIARIMRNELGVLGTLARWGGEEFGVLFEGLALEEA
                     RRRCERLRWAVERLDCSGFAPGWKMTISGGVAERTGIAHYERLVSRADALLYEAKRAG
                     RNRIFG"
     gene            complement(4930..5499)
                     /gene="orn"
                     /locus_tag="PK818_06330"
     CDS             complement(4930..5499)
                     /gene="orn"
                     /locus_tag="PK818_06330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HQY82384.1"
                     /translation="MHADSPNDRLIWIDLEMTGLDTDRDGILEIATVVTDGQLNLLAE
                     GPELAIAHPLATLEAMDDWNRTQHGKSGLWRRVLEEGVPLAEAEARTLAFLDQWVPAK
                     ASPMCGNSICQDRRFMHRLMPALEAHFHYRNLDVSTIKELARRWSPEVLAGVQKEAAH
                     TALSDVRDSIAELRHYRNSMGMLAGLPAG"
     gene            5683..6789
                     /locus_tag="PK818_06335"
     CDS             5683..6789
                     /locus_tag="PK818_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82385.1"
                     /translation="MAWMSYADGRGRGWAVDPDGCAHIFRSNHYNRQLVARSRVVTTS
                     QGLFLPTLTQVETNFSGIREATTQAAAANVRSIVSDAANDPRRFYEYLVHVREDGESA
                     GTHYRDMSRRATRATAASIDSHVTGWENALAAARFVRDASAGVLMVGATALSGGAALA
                     VGAAGTGITFTGNTQDNLAANQTMRQAMGNAAISTSVSVVTNILIPRGLSAVGRGMVG
                     AGQQLSVGQNVVLGLITVQANIAGDMFKTALTADQNIGPEARAQASRQLQAQVGARAA
                     TEMASMLFGAWLQTRGVPVGATIEVREAISGMRDSISGGLLSALGDRVVTAMSSQDAA
                     PGTGPGRDLDLAFTQLRGIVEAERYVREVAMRPL"
     gene            complement(6800..7300)
                     /gene="tadA"
                     /locus_tag="PK818_06340"
     CDS             complement(6800..7300)
                     /gene="tadA"
                     /locus_tag="PK818_06340"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009602240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HQY82386.1"
                     /translation="MVVNPEAQTEHDQRWMRQALALAGRAQREDDEIPVGALVVDADG
                     RLVAEGWNRNIAERDPSAHAEIVAMRRAGAAIGNHRLLGCTLYVTLEPCAMCAMAMVH
                     ARVARVVFGAFDPKTGAAGSVFDLLADPRHNHQVEVVGGVLGEEAGALLTGYFRAKRG
                     KPVDPG"
     gene            complement(7380..8960)
                     /gene="guaA"
                     /locus_tag="PK818_06345"
     CDS             complement(7380..8960)
                     /gene="guaA"
                     /locus_tag="PK818_06345"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HQY82387.1"
                     /translation="MTNIHTDKILILDFGAQYTQLIARRIREIGVYCEIWAWDHDPAE
                     IAGFGAKGIILSGGPESTTEAGAPAAPSQVFDSGLPLLGICYGMQTMAAQLGGATEAA
                     DQREFGHAQVEIVAGDALFSGLSDHCGAPCIDVWMSHGDHVATVPPGFAITATTDRIP
                     VAAMSNEDKRWYGVQFHPEVTHTKQGQELLRRFVVDVCGCRTLWTAANIIDDQIARVR
                     EQVGSDEVILGLSGGVDSSVVAALLHKAIGDQLTCVFVDTGLLRWNEGDQVMAMFANE
                     ADNGGMGIKVIRVNAADRYFDKLAGVSDPEAKRKVIGGLFVEIFDEESNKLANAKWLA
                     QGTIYPDVIESAGSKTGKAHVIKSHHNVGGLPEHMKLGLVEPLRELFKDEVRRLGVEL
                     GLPREMVYRHPFPGPGLGVRILGEVKRDYAELLAKADAIYIDELRKAGLYDRTSQAFA
                     VFLPVKSVGVVGDARAYEWVIALRAVETIDFMTAHWAHLPYDFLGTVSNRIINELRGV
                     SRVVYDISGKPPATIEWE"
     gene            complement(9125..>9462)
                     /locus_tag="PK818_06350"
     CDS             complement(9125..>9462)
                     /locus_tag="PK818_06350"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004657166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HQY82388.1"
                     /translation="FQGRSYKSYRGMGSLGAMEKGSKDRYFQDSSDADKLVPEGIEGR
                     VPYRGPLSGVVHQLAGGLRATMGYVGCATIEDMRKTPSFVKVSGAGQRESHVHDVQIT
                     KEPPNYRAG"
BASE COUNT         1424 a         3376 c         3170 g         1492 t
ORIGIN      
        1 tgctgccgaa cgccggcatc ggcctggcgc ggctggagat gatcatcgcc agccatatcg
       61 gcgtgcatcc gaacgcgctg ctggactatg cccgccagga cgccgcgacc aaggccagga
      121 tcgaagcccg caccgccggc tacgccggcc cggtcgagtt ctacgtggac cggctggccg
      181 agggcatcgc caccatcacc gcctcggtgg cgccgaaccc ggtgatcgtg cgcatgtccg
      241 acttcaagtc gaacgagtac gccaacctga tcggcggcgg caagtacgag ccgcacgagg
      301 agaatccgat gatcgggttc cgcggcgcct cgcgctacat cgacccgagc ttcaagcagg
      361 cgttcgcgct ggaatgccgc gcggtcaagc gcgtgcgcga ggacatgggc ctggacaacc
      421 tgtgggtgat gatcccgttc gtgcgcaccg tggacgaggg ccgcaaggtg ctggaggtgc
      481 tggcggaaaa cggcctgcgc cgcggcgagg gcgggctcag ggtcatcatg atgtgcgagg
      541 tgccgtcgaa cgcgctgctg gccgacgaat tcctcgacct gttcgatggc ttctcgatcg
      601 gttccaacga cctgacccag ctgacccttg gcctggaccg cgattcggcg atcgtcgccg
      661 gcctgttcga cgaacgcgac ccggcggtca agaagctgct ggcgatggcg atcagcgcgg
      721 cgaagcgacg gggcaagtac atcggcatct gcggccaggg ccccagcgac cacccggacc
      781 ttgcgctgtg gctgatggaa cagggcatcg agtcggtctc gctgaacccg gacaccgtgg
      841 tcgacacctg gctggcgctg gcgaaggccc gcgccggctg agcgccgcgc ggccgccacc
      901 gcggccgtgc caacatcaag gcggatcctg catgcccgct ccggggcatg cacgcaccga
      961 ggagactgca tgctgcctgc caccggcacc acccccgcgg ccccgcgcga agccgccagt
     1021 gccctggtca gcgaactgcg cgggatcgac tggctgcacc tgctggaggc gtggggtctg
     1081 cgcctgctgg cggcggtggc gatcttcgtg gccggccgct ggctggcgca gcggctgggc
     1141 aatggcctgg accgggtgat ggcacgcgcg ggggtggatc ccaccctcgg cggcttcctg
     1201 cgcaacatcg cctatgcggt gatgctggta ctggtgatca tgaccgcgct gaccgcgctg
     1261 ggggtcccca ccacctcgat gttcgcgatc ctcggcgccg ccggcctggc ggtgggcctg
     1321 gcactcaagg attcgctgtc caacatcgcc tcgggggtga tgctgatcgt gctgcggccg
     1381 ttccgcaccg gcgaccacgt cgtcgccgcc ggccaggaag gcacggtgct ggaggtgcgc
     1441 gtgttccaga cccggctgcg cgggctcgac caccgggtga tcatcctgcc caacagcgag
     1501 atcaccaccg cgccgatcat caattattcg agcctgccgc agcggcgctt cgacgtcacc
     1561 gttggcgtcg gctacaggga cgacctgcag caggcgcgcg acctgctggt gcggatcgcc
     1621 cgcgacgagc cgctggtgct ggacgaaccg gcgccggtgg tgcgggtgct gaacctcggc
     1681 gagagcagcg tggacctggt gttgcaggca ttcgccgcga acgacgattt catcgaggcg
     1741 cgcagccggg tggtggaagc ggtgcgcaac cagctgatcg ggcacggcct cagcatcccc
     1801 tatccgcagc gcgacctgca tgtgtaccac cgcgatgccg atggccggcc gctgtcggag
     1861 ctgctgctgc gcggcgtcgc cgacgacggc gaccaccggc cccggccgcc gcgcggctga
     1921 gcgcgccgat cagccgaaga tgcggttgcg cccggcgcgc ttggcttcgt acaacagcgc
     1981 gtcggcgcgg ctcaccaggc gttcgtaatg ggcaatgccg gtgcgttcgg ccaccccgcc
     2041 gctgatcgtc atcttccagc ccggcgcgaa gccgctgcag tccagccgct ccaccgccca
     2101 gcgcaggcgc tcgcagcggc gcctggcctc ttccagcgcc agcccctcga acagcacccc
     2161 gaactcctcg ccgccccagc gcgccagcgt gcccagcacg cccagctcgt tgcgcatgat
     2221 ccgcgcgatc tcgaccagcg cgcggtcgcc ggcggcgtgc gagtagccat cgttgatgcg
     2281 cttgaagtgg tcgatgtcga tcagcgcgaa gctcagtggc cgcccggact gcaccgcctg
     2341 ttcgaacgcg cgcgccagct cctcgtccat gctgcggcgg ttgccgaggc cggtgagcgc
     2401 gtcctcgcgg gcctgccgtt cgaacgcctc ggattgttcc cgcagcttgt cgaccaggaa
     2461 tgatttctcg cggtcggatt cgcgcaggcg ttcggtctgc tcgcgcaggt cgcgggtgcg
     2521 tgccgccacc accgcctcca ggtccgcggc gcgctgccgc atgtgcccca gccgccagcg
     2581 gtacaggccc gccagcgcca gcaccccgaa cagcagcagc aacggcagga accacggccg
     2641 ctgccagagc cgcggcgcga tcccgatctc cagggtggtg acgtccgggc tccagccgtg
     2701 gccaagcccg ggcgcggagg cattcacgcg gaaccggtag ttgccgggcg ccacgttggt
     2761 gtactgggcc acgcgctggt tgccgcgttc gatccagtcg cggtccacgc cctgcaactg
     2821 gtagcggtag cggacgaagc gcggggtctg gaaggtcagg ctggcgtaat ggaattcgag
     2881 cttgcgagtg cccgccggca attccaggcg gccctgtacc tgcacgtcgc ggtcgttggc
     2941 cagcacctgt tcgaccagca ccggcggcag cgtccgtttg taggcacgca gcgccttcgg
     3001 ctccaccacc gccgcgccac cggcggttgc gatccagatc cggccgccgc ggtcgcgcag
     3061 cgcggccgga ccggaaccac cgttgcactg gtagctggcc aggccatcgg cttcgccgaa
     3121 gcggtcgagc tccagccgcg gcttgcggcc atccatcacc gcctcgacct ccgagcgccc
     3181 gacccgcatc acccctcggt tggaggtcag ccacaggctg ccgatgccgt cgtcgaccac
     3241 cgcgaacagg gtatcgaccg gaagcccctg cgccagcccg agcgcctgca ggcggccgcc
     3301 ccggtagcgc agcaggccgc ggtcggtggc gaaccagagc gtgccgtccg cgtcttcgtg
     3361 gaaatcgaac acgtcctgcg cgccgttcat gccgcgcatg tccagcgcct gcacgcggcc
     3421 atccacgatc cgcgccgcac cgttggcggt gccgacccac aggctgccgt cgcgcgcgac
     3481 ctgcagcgaa atgatgaatt cgcgcggcag gccctgggcc acgccgaaat gggtgtacgc
     3541 gccgtcgcgc atccgcacca gcccgcggtt ggtgcctaac cacagggtgc cgtccgcctg
     3601 ctgcgccagc gcgcgcacct ggttgctgcc gggcatgccg tgcgcgttgt cgtagtgcgc
     3661 gaccagcttg ccgtcgcgca ggcgcatgac cccggcggtg tagctgccga ccagcaggct
     3721 gccatcgcgg tcttccagca ggctcagcac cgagtcgctg ggcaggccgt cggcggcggt
     3781 gtagctggcc tcgagcctgc caccgcgcca gcggttgagg ccgcggctgg tgccgagcca
     3841 cagcgacccg tcgcgcgact ccagcacggc gcgcacgtag tcgtcgctga gcccgtggtc
     3901 gccgttgtag gtgctgaacg gcgcgtcgct caggcgcagc aggccggcat tggtgcccgc
     3961 ccagatgctg ccctcgcgat ccaccagcag tgcggccacc cggttgttcg gcaacccgcg
     4021 caggctggtg aaggtctcga ccccggactt gcccagccgc aacaggccgt tgttgacgct
     4081 gccgacccac aggttgccgg cgccatcctg cgccatgctg ggcacgccat cgtccggcag
     4141 caacgccgag gcaagcacga agcgctcgcc ctggcgccgg tacacgccac gctcggtgcc
     4201 gacgaacagg cgtccgccgg catcctggaa cacccggaac accggtgccg gcggcaagcc
     4261 ggaggcctgc gcgtacacca ccaggcgccc gtccaagaaa tgcaccagcc cgcgcgcggt
     4321 tgccgcccac acgctgccgt cgcggtccag cagcaggccg taggtgaccg cgcttggcag
     4381 gccgttgcgg gtggtgaaat ggtcgacgcg gctgccgtcg acgacgtcga tgccggcatt
     4441 ctcggtcgcg acccacagcc gcccgcggcg gtcgaagatc gcgtccatga tctcttcctg
     4501 ggcaagcccg tccttgaccc gccaggttcg ccagtggtcg ccgcgcttga ccgtgacccc
     4561 gccgcgcgag gtaccgatca ccagcgcacc gtcggcggag gcgcgcaccg accgcacgcc
     4621 gttgtccttc agttccggcg tgttgccgcg atcgaaggaa tggaattcaa gcccgttgta
     4681 gcggaccagc ccttcccagg tgccgaacca caggtagccg tccggggtct gcgcgatcgc
     4741 gttgacctgg ttgtgcggca gcccctggcg ggtggtccag gtctcccgga agtaagccgt
     4801 cagcggcttg tcctgggcgc gggcgatggc cggcaacgcg ccgacgcaca gcagcaaggc
     4861 cagcatcgcc agcgcacgcg cccggttcgc aacagatgcc ccccgcatcc gttccatttt
     4921 cccctgcctt cagcccgccg gcaggccggc aagcatcccc atggagttgc gatagtggcg
     4981 cagttccgcg atggagtcgc gcacgtcgct cagtgcggtg tgcgcggcct ccttctgcac
     5041 cccggccagg acctccggcg accagcgccg cgccagctcc ttgatggtgc tgacatcgag
     5101 gtttcgataa tggaaatggg cctcaagcgc cggcatcagc cgatgcatga aacgccggtc
     5161 ctggcagatc gaattgccgc acatcggcga cgccttcgcc ggcacccact ggtccaggaa
     5221 cgcgagggtg cgcgcctcgg cctcggccag cggcacgccc tcctccagca cccgccgcca
     5281 caggccggac ttgccgtgct gggtgcggtt ccagtcgtcc atcgcctcca gcgtggccag
     5341 tggatgggcg atcgccagct ccgggccttc cgccagcagg ttgagctggc catcggtgac
     5401 cacggtggcg atctccagga tgccgtcgcg atcggtatcc agcccggtca tctcgaggtc
     5461 gatccagatc aggcggtcgt tcggggaatc ggcgtgcatg cgcggaccgg tttcggtggt
     5521 cgcgccatca tagcggcttg ccgcggtagc acgttttccg gccatcttcc gttgggctta
     5581 tgccctccag cggatgggca gccagcccgc cgcgctgcca tcctctgcca cgacttgcgt
     5641 ggcgtcccgc tgcgtgggca gcgatggatg gaggattccc agatggcttg gatgagttat
     5701 gccgatggcc gcgggcgcgg ctgggcggtc gacccggatg gctgcgccca catcttccgc
     5761 tccaatcatt acaaccggca gctggtcgcg cgtagccggg tggtcaccac cagccagggc
     5821 ctgttcctgc cgaccctgac ccaggtcgag accaacttca gcggcatccg cgaagccacg
     5881 acccaagccg ccgccgccaa cgtccgcagc atcgtcagcg acgccgccaa cgatccgcgg
     5941 cgcttctacg aatacctggt gcacgtgcgc gaggatgggg aaagcgcagg aacgcactac
     6001 cgggacatgt cgcggcgagc gacccgggcg accgcggcat cgatcgacag ccacgtcacc
     6061 ggctgggaaa acgcactcgc ggcggcccgc ttcgtgcgtg acgcctccgc cggcgtgctg
     6121 atggtgggtg ccaccgcgct gtcgggtggt gcggcgctgg cggtgggcgc cgccggcacc
     6181 ggcatcacct tcaccggcaa cacccaggac aacctggcgg ccaaccagac catgcgccag
     6241 gcgatgggca atgcggcgat cagcaccagc gtctcggtcg tcaccaatat cctgatcccg
     6301 cgcggcttga gtgcagtggg gcgcggcatg gtcggggccg ggcagcagct gtcggtcggc
     6361 cagaacgtgg tgcttggcct gatcacggtg caggcgaaca tcgccggcga catgttcaag
     6421 accgctctca ccgccgacca gaacatcggg ccggaggcgc gcgcgcaggc gtcgcggcaa
     6481 ctgcaggcgc aggtcggcgc acgcgccgcc accgagatgg ccagcatgct gttcggcgca
     6541 tggctgcaga cccgaggcgt gcccgttggc gccaccatcg aggtgcgcga ggcgatttcc
     6601 gggatgcgcg attcgatcag cggcggcctt ctgtctgcct tgggcgaccg cgtggtcacg
     6661 gcgatgtcca gccaggacgc cgcaccgggc accggcccgg gacgcgacct cgacctcgcc
     6721 ttcacccaac tgcgtggaat cgtcgaagcc gagcgctacg tgcgcgaggt ggcgatgcgg
     6781 ccgctgtgaa cgggcgtgct cagccgggat cgacgggttt gccgcgcttc gccctgaaat
     6841 agccggtcaa caaggcgccg gcctcctcgc ccagcacccc gcccacgacc tcgacttgat
     6901 ggttgtggcg cggatccgca agcaggtcga acacgctgcc cgcggcaccg gtcttgggat
     6961 cgaaggcacc gaataccacc cgcgccaccc gtgcatgcac catcgccatc gcgcacatcg
     7021 cgcacggctc cagggtgacg tacagcgtgc agccaagcag ccggtggttg ccgatggcgg
     7081 cgccggcgcg gcgcatcgcc acgatctcgg catgcgcgga cggatcgcgc tcggcgatgt
     7141 tgcggttcca gccttcggcc accagcctgc cgtccgcatc gacgaccaac gcacccaccg
     7201 ggatttcgtc gtcctcgcgt tgcgcacgcc cggccagcgc cagcgcttgg cgcatccagc
     7261 gctgatcgtg ctcagtctgc gcctcggggt tcacaacgat cctcgcctcc ttgccccggc
     7321 cccgcctgtt cggcggggca atgaacggcc tgcgaaccag tggttcgcaa gcaggccgtt
     7381 cattcccact cgatggtcgc cggcggcttg ccggaaatgt cgtagaccac gcgcgacacg
     7441 ccgcgcagtt cgttgatgat gcggttggag acggtgccga ggaagtcgta cggcaggtgc
     7501 gcccagtgcg cggtcatgaa gtcgatggtc tccactgcgc gcagcgcgat cacccattcg
     7561 taggcgcgcg cgtcgccgac cacgcccacc gacttcaccg gcaggaacac cgcgaaggcc
     7621 tggctggtcc tgtcgtacag gccggccttg cgcagttcgt cgatatagat cgcgtccgcc
     7681 ttggccagca gttcggcgta gtcgcgcttc acttcgccga ggatgcgcac gcccaggccc
     7741 gggcccggga acggatggcg atagaccatc tcgcgcggca ggccgagttc cacgccaagc
     7801 cggcgcactt cgtccttgaa caactcgcgc aacggttcga ccaggcccag cttcatgtgc
     7861 tcgggcaggc cgccgacgtt gtggtggctc ttgatgacgt gcgccttgcc ggtcttgctg
     7921 ccggcggatt cgatcacgtc cgggtagatc gtgccctgcg ccagccactt cgcattggcg
     7981 agcttgttgg actcctcgtc gaagatctcg acgaacagac cgccgatgac cttgcgcttg
     8041 gcttccggat cgctgacccc ggcgagcttg tcgaagtaac gatccgccgc gttgacgcgg
     8101 atgaccttga tgcccatgcc gccgttgtcg gcctcgttgg cgaacatcgc catgacctgg
     8161 tcgccttcgt tccagcgcag caggccggtg tccacgaaca cgcaggtcag ctggtcgccg
     8221 atcgccttgt gcagcaacgc cgcgaccacc gacgaatcca cgccgccgga caggcccagg
     8281 atcacttcat cggaaccgac ctgctcgcgc acgcgggcga tctggtcgtc gatgatattg
     8341 gccgcggtcc atagcgtcct gcagccgcag acgtccacca cgaaccggcg cagcagctcc
     8401 tggccctgct tggtgtgggt gacctcggga tggaactgca cgccgtacca gcgcttgtct
     8461 tcgttcgaca tcgccgccac cggaatacgg tccgtggtgg cggtgatggc aaagcccggc
     8521 ggcaccgtgg cgacgtggtc gccgtggctc atccacacgt cgatgcacgg cgcgccgcaa
     8581 tggtccgaca gccccgagaa caaggcatcg ccggcgacga tctccacctg cgcatggccg
     8641 aactcacgct ggtccgcggc ttcggtggca ccgcccagct gcgccgccat cgtctgcatg
     8701 ccgtagcaga tgcccagcaa cggcaggccg ctgtcgaaca cctgcgacgg cgcggccggc
     8761 gcgcctgctt cggtggtcga ttccgggccg cccgagagga tgatgccctt cgcgccaaag
     8821 ccggcgatct cggccggatc gtggtcccac gcccagattt cgcaatacac gccgatctcg
     8881 cggatgcggc gcgcgatcag ctgcgtgtac tgcgcgccga agtccaggat caggatcttg
     8941 tcggtgtgga tgttggtcat gggcttgggt cgcactctcg acttcgattg gctactcgtt
     9001 gccatccatg gcgctggcag ttctgctgcg acatgatcca gcccggccat ccatggccgg
     9061 gcgttcggcg acgcgcgaac cagtggttcg caaacgcgtc gcctcacccc ttccgtggac
     9121 tgcctcaacc ggcccggtag ttgggcggct ccttggtgat ctgcacgtcg tggacgtggc
     9181 tctcgcgctg gccggcgccg ctcaccttca cgaatgacgg cgtcttgcgc atgtcctcga
     9241 tggtggcgca gccgacataa cccatcgtcg cgcgcaggcc gccagcgagc tggtggacca
     9301 cgccgctcaa cgggccgcgg tacggcacgc ggccttcgat gccttccggc accagcttgt
     9361 cggcatcgct ggaatcctgg aagtagcgat ccttgctgcc cttctccatc gcgcccagcg
     9421 agcccatgcc gcggtagctc ttgtagctgc ggccctggaa ca
//
LOCUS       DAORUK010000074         9398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_7873_length_9398_cov_17.518998, whole genome shotgun sequence.
ACCESSION   DAORUK010000074 DAORUK010000000
VERSION     DAORUK010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 9398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9398
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7873_length_9398_cov_17.518998"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..363)
                     /locus_tag="PK818_06355"
     CDS             complement(214..363)
                     /locus_tag="PK818_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82389.1"
                     /translation="MASLVGDGDHLMQCQRYIESNPSRAAMIADPRDDRGPSQHALAF
                     ARPAR"
     gene            409..798
                     /locus_tag="PK818_06360"
     CDS             409..798
                     /locus_tag="PK818_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008095668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HQY82390.1"
                     /translation="MTTTMPMSQDAKLDRIVAQARRDAEQRDQGYRERALKMYPWVCG
                     RCAREFTRANLRELTVHHRNHDHDDNPPDGSNWELLCVYCHDNEHSRQTDYTGVSAQA
                     AEDKPAAATSNPFAGLKSLLGKGGAGP"
     gene            951..1211
                     /locus_tag="PK818_06365"
     CDS             951..1211
                     /locus_tag="PK818_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82391.1"
                     /translation="MTPHLLNVVDLAKQAETGVNVDWHLRDAVARTLDDLGHQFNAAE
                     LLAAYVQGLETAAQEAARGRAAYAGVLQTAAGIAAQRLKRRD"
     gene            1260..1754
                     /locus_tag="PK818_06370"
     CDS             1260..1754
                     /locus_tag="PK818_06370"
                     /inference="COORDINATES: protein motif:HMM:NF020136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HQY82392.1"
                     /translation="MRPLIGFDGGPGEPHWVALNDGVMGGCSTGAPAIADGRLEFRGA
                     LSLANNGGFASVRSVGRHFDLSGATAVVLRVLGDGRRYQLRLATDARHRGIAVSFGAG
                     FDTIAGQWIEVRVPLDGLQASVRGSLLEGQTMDPARVREIGLLVADKREGAFALSVDW
                     IALD"
     gene            1923..2816
                     /locus_tag="PK818_06375"
     CDS             1923..2816
                     /locus_tag="PK818_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016905110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HQY82393.1"
                     /translation="MSRVALYSPEPARGWLPWIWLVPILMILFNAVPVIALDDWMEAR
                     QWSTPRGDPIGLPGLHALLWIGFVPTLLLVLAWVRFVERRSLASIGLVGPAPLKTFAQ
                     GLAIGMGTIALVVSAIWAAGGLRATGWGLAWQSPQSLLQVALLLPGFMLQSSVEEVMF
                     RGWMQSVLARKAGVAVAVVLVALVFTLLHFSPNQRWLVMLSSFLFSLFASAWALRTGN
                     VWGVMGWHAGWNWLLATGFELPVTGIDAQLPALLVALQPSGVDALTGGAEGPEGSYLC
                     SVFFILAIARIGARRTGRGSR"
     gene            complement(2808..3914)
                     /locus_tag="PK818_06380"
     CDS             complement(2808..3914)
                     /locus_tag="PK818_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HQY82394.1"
                     /translation="MRDDALGQKIANLHDRIQGDVGNVDRIGIALYNPASDQLSTFLH
                     SADGQSPLERYQVRLADVPSLQELHRTQSSRTIDDLAILSASKSEHTRRILDAGYRSS
                     YTCPLYARGQLLGFLFLDSTRTGHFRNELRIQLDAYAQLIAAQISQDLSPARALRSAV
                     AMTRELGRHRDEETAGHVNRVAHYTRSIAIALDPGTGLSDEDIEFLFQFAGLHDIGKI
                     AIPDQILRKAGKLSEDEYAIAQSHVHKGGEMIDVMMREFGLANEHHAQMLRDVIACHH
                     ERWDGSGYPRGLAGEDIPLAGRIVAVADVFDALTNPRPYKQAWSLEQGFEYLQANAGT
                     QFDPNCVATAERCFDDWRQIYARFNEPTPATASA"
     gene            complement(4075..4947)
                     /locus_tag="PK818_06385"
     CDS             complement(4075..4947)
                     /locus_tag="PK818_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82395.1"
                     /translation="MTFQQIQTPVQEVGHVRFKIAAAATFGAETPSITIPGGSSTSLD
                     ELATSLASQPTAQNFASTLLDLAPHPSFAVTSWARLDTVFDSDWCFFRVAITLPTDEL
                     RRQVGSQHLAVSAGVSLMFGPSRAAWLAIAMRLGPQFGAFLARIMPTIAPEVAAGGSA
                     FSNWINPVGMAIPLGIAVRDFVVAICAAARARGIARGHWNQVGTAYAWHVYGMSSGRH
                     FSNAAAAEVGAARARADIERHGAARLRGYLEQHFREGRAMPSRHTQAGEFDDREVLQV
                     GFELGEMMFRATEP"
     gene            complement(5065..5331)
                     /locus_tag="PK818_06390"
     CDS             complement(5065..5331)
                     /locus_tag="PK818_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82396.1"
                     /translation="MSDDGGLGLLLLGPAGAAGLYWMLYRYYRNTDKSHAFERETTVD
                     AKPVTGDHDDHKVDEVKGTRETRIRGDNVSEYRKRVQRIGGDTS"
     gene            complement(5331..6650)
                     /locus_tag="PK818_06395"
     CDS             complement(5331..6650)
                     /locus_tag="PK818_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009602102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HQY82397.1"
                     /translation="MSNANTPPPLPPSVPPPTGPGSPRDVPPLPGSHGIDPESLPEPI
                     REELLAPDPVAIDTAAEELKDGQNHCPKCGATDIRHKTGSDLLVCLYCRNEWHGQRVE
                     EEFGLGEGIDQLKGTVIASGAQDIAADAESLMSFKCSGCGAEVTVNTENAMTARCHWC
                     RHVFGVNEQVANGAVPDAVLPFHIKKADAVARIRQFVDKRRLFALKAFKEQFTPENVV
                     AVYMPYMIVDGNASVSVAGHGEIETRRYTQGSGKDKKTYYDADVYAIERHVDFTVDDL
                     PLESSGERGNLDTSSNTNNIINTILPFDTKNAVKWNASYLNGVSSEKRDLDVEHLHPR
                     LEDQLLSIARCQVEPSVSRFDRGVRWEQERLDVHGTRWVSMYLPVWLYSFHQPGSNGG
                     MLHYIAVNGRTGETMGSVPVQQWKLLAAAITTGTFLEAIALMIIGSQ"
     gene            complement(6673..7824)
                     /locus_tag="PK818_06400"
     CDS             complement(6673..7824)
                     /locus_tag="PK818_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012511156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQY82398.1"
                     /translation="MGLVQAVVGAVGGMLADQWKDFFTVPDGLPPTAALFAAVPHGTN
                     AGRGSNTKGSANIITNGSKIVVPEGYGLLLFQDGKITGFAAEPGGYEWRSDDINSQSI
                     FAGDGIVSPLIKQSWERFKFGGQPGSQQAAFFVSLKELPDNRFGTQSEIYWDDGFLGT
                     QVGAVTRGSYTLKIADPILFVKNFVPATYLQPGQVFDFTDLDNAAAAQLFNEVVGSLA
                     PAFSLYTNDPAKGNRITKLQQDSVGFAKSLSDAVENAYQWKSDRGLVIAKTAIVSIEY
                     DANTRELLKTVQRADALAGARGNSNLQASVAAGMQAAGENGGAAGMMGIGMASGMMGG
                     LGSLQQPVAPAAPAAEDPVAKLKKAKEMLDLGLITQGDYDALKAKALGL"
     gene            complement(7955..8548)
                     /gene="trmY"
                     /locus_tag="PK818_06405"
     CDS             complement(7955..8548)
                     /gene="trmY"
                     /locus_tag="PK818_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (pseudouridine(54)-N(1))-methyltransferase
                     TrmY"
                     /protein_id="HQY82399.1"
                     /translation="MRTFVLRARAASTDSQKLLASVGKDAHAEILAHTLMNAVFVAQS
                     HRPDVVVYLVLESTQDYSRTIRFEPDAMHDIGGFDERALLGKIARALDASRGMAKEEA
                     REVESGVGVRTVAFERLVQELAAGHQLFVMDPKGTPIREQPFQDNPCFLLTDHIPMPK
                     KSFNSLERLGAKKLTLGSKMLFASQCVVLIHHELDQR"
     gene            complement(8586..8744)
                     /locus_tag="PK818_06410"
     CDS             complement(8586..8744)
                     /locus_tag="PK818_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82400.1"
                     /translation="MAKPNYSFEKRQREIAAKKQKEEKLAKKQQAREAAKAANQPAGE
                     HTPSDPLE"
     gene            8896..9261
                     /locus_tag="PK818_06415"
     CDS             8896..9261
                     /locus_tag="PK818_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6164 family protein"
                     /protein_id="HQY82401.1"
                     /translation="MSRLLLNLRNVPDDEADDVRALLDSNRIAFHETRPSMWGISAGA
                     IFVTEDASIPEAKRLMAGYQEQRRIRARAEYAAAVRDGTAETFWSLLRAEPGRVLLTL
                     LAIAFLLGLVALPVYLLRG"
     gene            9325..>9398
                     /locus_tag="PK818_06420"
     CDS             9325..>9398
                     /locus_tag="PK818_06420"
                     /inference="COORDINATES: protein motif:HMM:NF018958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HQY82402.1"
                     /translation="MINNDVLRSIRYMLDLSDNKVIEV"
BASE COUNT         1394 a         3068 c         3285 g         1651 t
ORIGIN      
        1 gatcagcgcg atcagtgcgg cgaagaagct gatcaggccg agttgcgggt cttcggttgc
       61 acgcttgact gcggcacgcc agccagtcgc gggcgcttcc ccgcgcgcgc gctgcccgct
      121 gccctgcggg gatgaccgct gcggtgagaa cccgttcatc ggatccaagc ataaaccgga
      181 aagcaggggt cagaaagtcg gtaggcggcg tgatcagcgg gccggcctcg caaaggccaa
      241 cgcatgctgg ctgggcccgc gatcgtcacg cgggtcggcg atcatggccg cgcgcgacgg
      301 gttcgattcg atgtagcgtt ggcattgcat caggtgatcg ccatcaccca ccaggcttgc
      361 cacgtaacgc actccccgaa aacgggatca ggtttacttg tcccacggat gaccacgacc
      421 atgcctatgt cacaagacgc caagctcgac cgcatcgttg cccaggcccg gcgcgacgcc
      481 gagcagcgcg accaggggta tcgcgagcgt gcgctgaaga tgtacccatg ggtctgcggc
      541 cgctgcgcgc gcgaattcac ccgcgccaac ctgcgcgagc tgacggtcca ccaccgcaac
      601 catgaccacg acgacaaccc gcccgacggc agcaactggg agctgttgtg cgtgtactgc
      661 cacgacaacg agcactcgcg ccagaccgac tacaccgggg tcagcgcaca ggccgccgag
      721 gacaagccgg ccgccgccac ctccaatccc tttgccggcc tgaagtcgct gctcgggaag
      781 ggtggagccg gcccgtgacc gcgtatgcgc tggcgtggac cggtcgtgtg gcgcgccacc
      841 ggcaagggga cgcatcgcac cacaccacgc gccgttaacg ccggatcgcg ctatcgtttc
      901 ggtcccgtgc agatcaggcc aggcaagtga aagtcacgat cgtgggtctg gtgacgccgc
      961 atctgctgaa cgtggtggac ctggccaagc aggccgagac aggcgtcaac gtcgactggc
     1021 acctgcgcga cgcggtggcc aggacgctgg acgacctcgg gcaccagttc aacgccgcgg
     1081 aattgctcgc ggcctacgtg caagggctgg agaccgccgc ccaggaggca gcgcgcggcc
     1141 gcgcggccta cgcaggcgtc ttgcagactg cagccgggat tgccgcgcaa cgcctcaagc
     1201 ggcgtgattg aggaagcggt gggccaaccg cgtccatcgc gtcggcccgg gaggccctga
     1261 tgcgcccgct gatcggtttc gacggtggcc ccggcgagcc gcactgggtg gcactgaacg
     1321 acggggtgat gggcggctgt tcgaccggcg ctccggcgat cgccgacggc aggctcgagt
     1381 tccgcggcgc gctctcgctg gcgaataacg gaggcttcgc ttcggtgcgc agcgtcggtc
     1441 gccacttcga cctcagcggt gccaccgcgg tggtgctgcg ggtgctgggt gatggccgcc
     1501 gctaccagct gcggctggca acggatgcgc gccatcgcgg catcgcggtg tcgttcggcg
     1561 ccggcttcga caccattgcc ggccagtgga tcgaggtgcg ggtaccactg gacggattgc
     1621 aggccagcgt gcgcggcagt ttgcttgaag ggcagacgat ggacccggcg cgggtgcgcg
     1681 aaatcggcct gctggtcgcg gacaagcgcg aaggcgcatt cgcgctttcg gtggactgga
     1741 tcgcactgga ctagcgaacg cagggacggg tttcgtcggc tgattgcccg gacttgtcga
     1801 tcggcgcttg ccagggtgag gcgtggtcgc caccgtgcag gctcgccgtg ccggcacgcg
     1861 cgcaggtccg ctggccgggt gcgtgcgcat gcggcgccat cgccaccgtt ccggagcgcc
     1921 acatgtcccg tgttgccctg tattcccccg aacccgcgcg cggctggttg ccctggatct
     1981 ggctggtacc gatcctgatg atcctgttca acgcggtgcc ggtgatcgcg ctggacgact
     2041 ggatggaagc acggcagtgg tccacgccgc gcggcgatcc gatcggcctg cccgggctgc
     2101 acgcgctgct gtggatcggc ttcgtgccga ccctgctgct ggtgctggcg tgggtgcgct
     2161 tcgtcgaacg gcggtcgttg gccagcatcg gcctggtcgg gccggcgccg ctgaagacgt
     2221 ttgcgcaggg gctggcgatc ggcatgggca ccatcgcgct ggtggtgtcc gcgatctggg
     2281 cggccggcgg cctgcgcgcc acgggctggg ggctggcgtg gcagtcgccg cagagcctgc
     2341 tgcaggtcgc gttgctgttg ccgggtttca tgctgcagtc cagcgtggag gaggtcatgt
     2401 tccgcggctg gatgcagtcg gtgctggcgc gcaaggcggg cgtggcggtt gcggtggtgc
     2461 tggtggcgct cgtgttcacc ctgctgcact tcagcccgaa ccagcgctgg ctggtcatgc
     2521 tgtccagctt cctgttctcg ctgttcgcgt ccgcatgggc gctgcggacc ggcaatgtct
     2581 ggggcgtgat gggttggcac gcgggctgga actggctgct ggcgaccggc ttcgagttgc
     2641 cggtgaccgg catcgacgca cagctgccgg cgctgctggt ggcgctgcaa cccagcggcg
     2701 tggacgcgct gaccggtggc gccgaaggtc cggaaggcag ctacctgtgc agcgtgttct
     2761 tcatcctggc gatcgcccgg atcggagcgc ggcggaccgg gcggggctca cgctgatgcg
     2821 gttgccggcg tcggctcgtt gaagcgcgcg tagatctgcc gccaatcgtc gaagcagcgt
     2881 tcggcggtgg cgacgcagtt cggatcgaac tgggtgccgg cgttggcctg caggtactcg
     2941 aagccctgct ccagcgacca tgcctgcttg taggggcggg ggttggtcag ggcatcgaat
     3001 acatcggcca ccgcaacgat gcgaccggcc agcgggatgt cctcgccggc caggccgcgc
     3061 ggatagccgc tgccatccca gcgttcgtgg tggcaggcga tgacgtcgcg cagcatctgc
     3121 gcatggtgtt cgttggcaag gccgaactcg cgcatcatca cgtcgatcat ctcgccgccc
     3181 ttgtgcacgt gggactgggc gatggcgtat tcgtcctcgc tcagcttgcc ggccttgcgc
     3241 aggatctggt cggggatcgc gatcttgccg atgtcgtgca ggccggcgaa ctggaacagg
     3301 aactcgatgt cctcgtcgct caggccggtc ccggggtcca gcgcaatcgc gatgctgcgc
     3361 gtgtagtggg cgacccggtt gacgtggccg gcggtttcct cgtcgcggtg gcggccgagt
     3421 tcgcgggtca tcgccaccgc gctgcgcaat gcacgggccg gggacaggtc ctgcgagatc
     3481 tgcgcggcga tcagctgggc ataggcgtcc agctggatcc gcagctcgtt gcggaaatga
     3541 ccggtccggg tggagtccag gaacaggaag cccagcagct ggccgcgcgc gtacagcggg
     3601 caggtgtagc tggaacgata gccggcgtcg aggatgcgcc gggtgtgctc gctcttcgag
     3661 gcggacagga tggccaggtc gtcgatggtc cggctggact gggtgcgatg cagttcctgc
     3721 agcgagggta cgtccgccag ccggacctgg taacgctcca gcggcgactg tccgtcggca
     3781 ctgtgcagga aggtgctgag ctggtcgctg gccgggttgt agagggcgat cccgatgcgg
     3841 tcgacgttgc cgacgtcgcc ctggatgcga tcgtggaggt tggcgatctt ctgtcccagc
     3901 gcgtcatccc gcatcgttgg ttcctgtcgc tggtctgctg gcgctgccaa aggcccaagc
     3961 atagcggggg aaacagggga aaacggcgac gcagtcagtc aattccgacc aatatcctgc
     4021 tcctggcgtg cacctgcgca cagacgggac ccgcgctgcg gccggtttcc ggggtcaggg
     4081 ttcggtggcg cggaacatca tctcgcccag ttcgaacccg acctgcagga cctcgcggtc
     4141 gtcgaattca ccggcctggg tgtgccgcga cggcatcgcc cggccctccc tgaagtgctg
     4201 ttccaggtag ccgcgcaggc gggccgcgcc gtggcgctcg atgtccgccc gcgccctggc
     4261 cgccccgact tcagcggccg ccgcattgct gaagtggcgc ccgctgctca tgccgtacac
     4321 gtgccaagca tacgccgtgc cgacctggtt ccagtggccg cgggcaatgc cacgggcacg
     4381 cgccgccgcg caaatggcga ccacgaagtc gcgcacggcg atccccagcg ggatcgccat
     4441 cccgaccggg ttgatccagt tcgaaaaggc gctgccaccg gcggcgacct ccggcgcgat
     4501 cgtgggcatg atcctggcca agaacgcgcc gaactgcggc cccagccgca tcgcgatggc
     4561 cagccacgct gcgcggcttg gaccgaacat cagggagaca cccgcggaaa cagccaaatg
     4621 ctgcgagccc acctgccgac gcaattcgtc ggtcgggagg gtgatcgcca cccggaagaa
     4681 gcaccagtcg ctgtcgaaca ccgtatccag cctggcccag ctggtgaccg cgaaactcgg
     4741 gtggggcgca aggtccagca gggtggacgc gaagttctgg gcagtgggct gcgaggccaa
     4801 ggacgtggcc agttcatcca ggctggtgct gctgccgccg ggaatggtga tcgagggcgt
     4861 ctccgctccg aaggtcgccg cggccgcgat cttgaaacgg acatgcccga cttcctgcac
     4921 cggggtctgg atttgctgga aggtcatggg cgaaggtcct cggttgcacg atcaccggcg
     4981 ctgcaggggc cgcgccacgc gccgcacagg caagcgcgag cgcgggtgaa cgggtggacg
     5041 ccaccacgcc gggtgggcca ggcctcagct ggtatcgccg ccgatgcgct ggacgcgctt
     5101 gcggtactcg ctgacgttgt cgccgcggat gcgggtctcg cgcgtgccct tcacctcgtc
     5161 gaccttgtgg tcgtcgtggt cgccggtcac gggcttggca tcgaccgtgg tctcgcgttc
     5221 gaaggcatgc gatttatcgg tgttgcggta gtagcgatac agcatccaat aaaggccggc
     5281 cgcgccggcc gggccaagca ggagcaggcc caacccaccg tcgtcgctca tcattggctc
     5341 ccgatgatca tgagggcaat tgcctccagg aacgtgccgg tggtgatcgc tgcggcgagc
     5401 agcttccact gctgcaccgg cacgctgccc atggtctcgc cggtgcggcc gttcaccgcg
     5461 atgtagtgga gcatgccgcc gttgctgccg ggctggtgga aggagtacag ccacaccggc
     5521 aggtacatcg acacccagcg ggtgccatgc acgtccaggc gttcctgctc ccagcgcacg
     5581 ccacggtcga agcggctgac cgatggctcg acctggcagc gggcgatcga cagcaactgg
     5641 tcctccagcc gcgggtgcag gtgctccacg tcgaggtcgc gcttctccga gctgacgcca
     5701 ttgaggtagg acgcattcca cttcaccgcg ttcttggtgt cgaacggcag gatcgtgttg
     5761 atgatgttgt tggtgttgct gctggtgtcg aggttgccgc gctcgcccga ggactccagc
     5821 ggcaggtcgt ccaccgtgaa gtcgacgtgc ctttcgatcg cgtaaacgtc ggcatcgtag
     5881 taggtcttct tgtccttgcc gctgccctgg gtgtagcgac gggtctcgat ctcgccgtgg
     5941 ccggcgacgg agacgctggc gttgccgtcc acgatcatgt acggcatgta gacggccacc
     6001 acgttttccg gggtgaactg ttccttgaac gccttcagcg cgaacaggcg ccgcttgtcc
     6061 acgaactggc ggatgcgcgc gaccgcatcc gccttcttga tgtggaacgg cagcaccgca
     6121 tcgggcaccg cgccgttcgc cacctgttcg ttgacgccga acacgtgccg gcaccagtgg
     6181 cagcgcgcgg tcatcgcgtt ctcggtgttg accgtgacct cggcgccgca gccgctgcac
     6241 ttgaagctca tcaggctttc ggcgtcggcc gcgatgtcct gcgcaccgga ggcgatgacc
     6301 gtgcccttga gctggtcgat gccttcgccc aggccgaatt cctcctcgac ccgttgcccg
     6361 tgccattcgt tgcggcagta caggcagacc agcaggtcgc tgccggtctt gtggcggatg
     6421 tcggtggcgc cgcacttcgg gcaatggttc tggccgtcct tgagttcctc ggcggcggtg
     6481 tcgatcgcga ccgggtcggg cgccagcaat tcctcgcgga tcggctccgg caatgattcc
     6541 gggtcgatcc cgtggctgcc cggcagcggc ggcacgtccc gcggcgatcc gggcccggtc
     6601 gggggcggca ccgacggcgg cagtggcggc ggcgtgttgg cgttcgacat ggaggcttcg
     6661 cgggtcggcg gcttacaggc ccagcgcctt ggccttgagc gcgtcgtagt cgccctgggt
     6721 gatcaggccg aggtccagca tctccttggc cttcttcagc ttggcgacgg gatcttccgc
     6781 ggccggcgcg gccggcgcca ccggctgctg caggctgccg agcccaccca tcatgccgga
     6841 ggccatgccg atccccatca tgcccgcggc gccgccgttc tcgccggcgg cctgcatgcc
     6901 cgcggccacg ctggcctgca ggttggaatt gccgcgcgca ccggccagcg catcggcgcg
     6961 ctgcacggtc ttcagcagtt cacgggtgtt ggcgtcgtat tcgatggaga cgatcgcggt
     7021 cttggcgatc accaggccgc gatcggactt ccactggtag gcgttttcca ccgcgtccga
     7081 caagctcttg gcgaagccca ccgaatcctg ctgcagcttg gtgatgcggt tgcccttggc
     7141 cggatcgttg gtgtacaggc tgaacgccgg tgccagcgaa cccacgactt cgttgaagag
     7201 ctgggcggcg gcggcattgt cgaggtcggt gaaatcgaag acctggccgg gctgcaggta
     7261 ggtggccggc acgaagttct tcacgaacag gatcgggtcg gcgatcttca gggtgtagga
     7321 gccgcgcgtc accgcgccga cctgggtgcc caggaagccg tcgtcccagt agatctcgga
     7381 ttgggtgccg aagcggttgt ccggcagttc cttcagcgac acgaagaacg ccgcctgctg
     7441 cgaacccggc tggccgccga acttgaagcg ctcccagctc tgcttgatca gcgggctgac
     7501 gatgccgtcg ccggcgaaga tcgactgcga gttgatgtcg tccgaacgcc attcgtagcc
     7561 accgggctcg gccgcgaagc cggtgatctt gccgtcctgg aacagcagca ggccgtaacc
     7621 ttccggcacc acgatcttcg agccgttggt gatgatgttg gccgaaccct tggtgttcga
     7681 gccgcgcccg gcattggtgc catgcggcac cgccgcgaac agcgccgcgg tgggcggcag
     7741 gccatcggga accgtgaaga aatccttcca ctggtcggcg agcatgccac caaccgcacc
     7801 tacgacagct tgaacaagac ccattgcatc tctccgcgat tcagtggccg ggcaccgtac
     7861 ccaccaagac ggggaaacta tacattcccg gccagccgcc cacgtaccgc ccgctgcggt
     7921 tgcgcgtgcg gcggcgaatc cggcgccggg tttgctagcg ctggtccaac tcgtggtgga
     7981 tcagcaccac gcattgggag gcgaacagca tcttcgatcc cagggtgagc ttcttcgcgc
     8041 cgaggcgctc caggctgttg aagctcttct tcggcatcgg gatgtggtcg gtcagcagga
     8101 agcaggggtt gtcctggaac ggctgttcgc ggatcggggt gcctttcgga tccatcacga
     8161 acaactggtg gcctgccgcc agttcctgca ccagccgctc gaacgcgacc gtgcgcacgc
     8221 cgacgccgga ttccacctcc cgcgcctctt ccttggccat gccgcgcgag gcatcaagcg
     8281 ccctggcgat cttgccgagc agcgcgcgtt cgtcgaagcc gccgatgtcg tgcatcgcat
     8341 cgggttcgaa gcggatcgtg cgcgagtagt cctgggtgct ttccagcacc aggtagacca
     8401 cgacatccgg gcgatgcgac tgcgccacga acaccgcgtt catcagcgtg tgcgccagga
     8461 tctcggcgtg ggcgtccttg cccaccgagg ccagcagttt ctggctgtcg gtggacgccg
     8521 cccgcgccct cagtacgaag gttcgcatgc ttgagttggc cgctgcatcc gctggccggg
     8581 gatgcttatt cgagcgggtc ggacggcgtg tgctcgcccg cgggctggtt ggcggccttg
     8641 gcggcttcgc gcgcctgctg cttcttcgcc agtttctctt ccttctgctt ctttgcggcg
     8701 atctcgcgct ggcgcttctc gaacgaatag ttgggcttgg ccaagggtgg ctgctccggt
     8761 cgtggggtgg cgcccagtgt agcggatgcc ccggcggcgg ccgttcgcgc atggtggacg
     8821 cacgcggggc accggcgggc gaagcatgcg ggcatgccgc ggatcccgtc acgggcatgc
     8881 cgctatcatc cggcgatgtc caggctcctg ctcaacctgc gcaacgtccc cgatgacgaa
     8941 gccgacgacg tgcgcgcatt gctggattcc aaccgcatcg cgttccacga aaccaggccg
     9001 agcatgtggg gcatttccgc gggcgccatc ttcgtcaccg aggacgcgag catcccggag
     9061 gccaagcgcc tgatggccgg ctaccaggag cagcgccgca tccgcgcccg cgccgaatac
     9121 gccgctgcgg tccgcgatgg caccgccgaa acgttctggt cgctgctgcg cgccgaaccc
     9181 gggcgggtcc tgctgaccct gctggcgatc gccttcctgc tggggctggt ggcactgccg
     9241 gtctacctgc tgcgcggcta ggcctgcact gcccgcgcac gacctcggta gactggcggc
     9301 accgcttgcc ccgatgccgg atcgatgatc aacaacgacg tgttgcgcag catccgctac
     9361 atgctggatc tcagcgacaa caaggtgatc gaggtcat
//
LOCUS       DAORUK010000075         9366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_7913_length_9366_cov_19.045860, whole genome shotgun sequence.
ACCESSION   DAORUK010000075 DAORUK010000000
VERSION     DAORUK010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 9366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9366
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7913_length_9366_cov_19.045860"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="PK818_06425"
     CDS             complement(<1..142)
                     /locus_tag="PK818_06425"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437778.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQY82403.1"
                     /translation="MHKIIVSGGARLHGEVRISGAKNAVLPVLCATLLADAPVRITNV
                     PRL"
     gene            complement(142..375)
                     /locus_tag="PK818_06430"
     CDS             complement(142..375)
                     /locus_tag="PK818_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HQY82404.1"
                     /translation="MNADTIQKLIEAGLPGARVAVQGADGVHFEAEVVCAAFAGKLPL
                     ARHRMVYATLGALMGGEIHALQLRTLTPDEAAA"
     gene            445..1437
                     /locus_tag="PK818_06435"
     CDS             445..1437
                     /locus_tag="PK818_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HQY82405.1"
                     /translation="MPPALPASDFDFATSGRRVFAVERDGLAAVAARIDGAFSSACTA
                     ILGGRGRVVCTGMGKSGHVARKIAATLASTGTPAFYMHPGEAAHGDLGMVTDADIVLA
                     LSYSGESDELLLLLPALRRQGNFVIAMTGRGLSTLAREADLHLDVSVPAEACPLALAP
                     TSSTTAALAMGDALAVALLEARGFTADDFARSHPAGALGRRLLLHITDVMHAGDEVPR
                     IGPGASINEALVEMSRKRLGMTAVVDDAGRLLGLYTDGDLRRSLDDAAVDLRTTRIDA
                     VMTRAPRTIDADALAVEAAQLMEAHKINALLVVDGDGRVVGALNIHDLLRARVV"
     gene            1443..1982
                     /locus_tag="PK818_06440"
     CDS             1443..1982
                     /locus_tag="PK818_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HQY82406.1"
                     /translation="MPHYPDALLQRAAKIRLVGFDVDGTLTDGRLHYASDGHETKSFH
                     VQDGMGLVLLREAGIELALVSARISPLVAARGSELKIAHVHVGERSKLACMQRIAAGM
                     GIGMDAVAFMGDDLPDLATLRAAGLAIAPANAHPWALAAAHWVAPRNGGEGAARDACD
                     LILQAQGRLEGILAHGEHP"
     gene            1979..2536
                     /gene="lptC"
                     /locus_tag="PK818_06445"
     CDS             1979..2536
                     /gene="lptC"
                     /locus_tag="PK818_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HQY82407.1"
                     /translation="MSWRGGLTVVLLLAAIACGWSVWRFTGGGAEAMASTRSEYVLHD
                     YEIVSLDSEGKESFTLSGPRLQRDPGAKSMSLETPRFLVPDRNGAYWDVRAQRGLVPD
                     DGKELRLRGRVVATSPPQAPPPTRIETEELNLFPRENRAASASTVTLTRPGLTMRGHG
                     MRADFDRQQVSLLSDVHTRYVPTPR"
     gene            2517..3080
                     /gene="lptA"
                     /locus_tag="PK818_06450"
     CDS             2517..3080
                     /gene="lptA"
                     /locus_tag="PK818_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HQY82408.1"
                     /translation="MSRRPADLAAAVLALSAAAFAAGAGARSSDRNQPMDIEAAHSTC
                     SLGANDSCTLTGNVTMTQGSLNVVAAKAIIEQSGGDPSRALLSGGVTLRQTLDDGNQI
                     DATASNVDYNLKTEVVVFTGNVEIRQLRGTLNGQRVVYNMKTNQVESGGTGNGRVKMR
                     ILPKSAQAQPAKAAAAPVVPAPPAGKR"
     gene            3080..3799
                     /gene="lptB"
                     /locus_tag="PK818_06455"
     CDS             3080..3799
                     /gene="lptB"
                     /locus_tag="PK818_06455"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005913374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HQY82409.1"
                     /translation="MLAASGLRKRYKSREVVRDFGLTLDAGEVVGLLGPNGAGKTTCF
                     YMIVGLVPADAGSIVLDGTDITGEPMYARAKRGVGYLPQEPSVFRKLSVADNIRLVLE
                     LRDDLDDDGRARELESLLDELQIGHVAEQMGASLSGGERRRVEIARALAGKPRMILLD
                     EPFAGVDPISVNEIQRIVRHLKDRGIGVLITDHNVRETLGICDRAYILADGAVLAQGA
                     PDALLENPDVRRVYLGDAFKL"
     gene            3883..5322
                     /locus_tag="PK818_06460"
     CDS             3883..5322
                     /locus_tag="PK818_06460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HQY82410.1"
                     /translation="MKPRLSPGLHQQLILTPQLRQAIRLLQLSTVELEAEVAEAVASN
                     PLLDWAEEGEPGPADAHEVRDADGIQEAPVREHTEERSDEHWDREGEPWQERIGPASD
                     PGDDDFGGAGERAASAETLRDHLLWQLHLGHFSQRDQGIGVALVDAIDEDGYLRDDLE
                     AIAAGLRPEIDAGLDDVLQVLHRIQQFDPVGVGARDLGECLRLQLRALAEDTPGRELA
                     MAVTAGALARLPRIGIDGLCQELDCTRDQAEAAVHLLRSLDPRPGAQHGELPAGTYVI
                     PDCVIWRQQGLWRVALAAGATPRITIQRDYEQMIRHAAASDADYLRGRLQEARWLLKG
                     LEARADTLLRVVRCLVREQAGFLEFGDQALHPLTLRTVAAELGLHESTVSRAIARKYV
                     RTPRGTIPLRDFFASGIDTGNGGSASSTAIQDMIRKLVEAEDPRKPLSDARLADTLKS
                     GGVPVARRTVAKYREALQIPASHERVRIG"
     gene            5383..5709
                     /gene="raiA"
                     /locus_tag="PK818_06465"
     CDS             5383..5709
                     /gene="raiA"
                     /locus_tag="PK818_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005913379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HQY82411.1"
                     /translation="MQIETHGQQLEVTPALRDYVETRFERLGRHFEHPCQIRVQLGLD
                     KPDHKAEATVSLSGRTLHADANAQDMYAAIDLLADKLDRLLLKQKERMVDHHRGEGIA
                     HVGTAD"
     gene            5741..6694
                     /gene="hprK"
                     /locus_tag="PK818_06470"
     CDS             5741..6694
                     /gene="hprK"
                     /locus_tag="PK818_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HQY82412.1"
                     /translation="MNAARITAAELFDQQAERLELRWLAGQRGGDGRMLEAVETVARR
                     PSLAGYLNIIYPNKVQILGTEELAWLDGLDSRQRWETIQKIIGFRPLALVISKNQSCP
                     EDLRIAAEESETPLWNSPRRGHELLNHLQYKLARTLAPRVTLHGVFMEIYSIGVLITG
                     ESGSGKSELALELVTRGHRLVADDAPEFTQIAPDVLDGACPDLLQDMLELRGLGVLNI
                     RQMFGDTAVKRNKYLRMIVHLSKPSLEAKPGGMERLTGDIGSRRLLDLDVPMITLPVM
                     AGRNLAVLTEAATRMHLLRSKGVDPAAAFMARHSHFLERSQ"
     gene            6691..7581
                     /gene="rapZ"
                     /locus_tag="PK818_06475"
     CDS             6691..7581
                     /gene="rapZ"
                     /locus_tag="PK818_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012479342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HQY82413.1"
                     /translation="MTTADPGPTPQALPPLVIVSGMSGSGKSVALNTFEDLGYYCVDN
                     LPAELLPEFVRRILARDNAPEKIAVGIDARSLGDLSELPEALSQVGAQGLDTRLLFFD
                     TRDDVLLRRYADTRRRHPLSHLGLVLADAISLERQALKPLRQIADSQLDTSDLNVHQM
                     RRLILTEYALSGAGLSLLFESFAYRRGVPAEADFVFDARVLPNPHWDARLRPLSGRDA
                     DVRDYLEAQPQVAEYVGQIQGFLDTWLPRLKSETRSYVTVAFGCTGGRHRSVYLAERL
                     ARHCREAGWAEVAVHHRELD"
     gene            7738..8130
                     /locus_tag="PK818_06480"
     CDS             7738..8130
                     /locus_tag="PK818_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017154797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose IIA subunit family protein"
                     /protein_id="HQY82414.1"
                     /translation="MAVGILLITHEGIGNALVAVATRLLRKLPLETEAFEVPFDGDLD
                     ALLPLASAALRRVDGGHGVLVLTDLYGATPSNLAARLARLGTPVRRVSALSLPMLLRV
                     MNYADLGLDELPAVAAAGARNGAITDDA"
     gene            8170..8439
                     /locus_tag="PK818_06485"
     CDS             8170..8439
                     /locus_tag="PK818_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HQY82415.1"
                     /translation="MIQRDLTIINRLGLHARATAKLVQLLSGFRCQATLEARGREVNA
                     KSIMGVMLLAAGTGSTVVLRVDGEDEQAAADAVAALFERRFDEDS"
     gene            8439..>9366
                     /locus_tag="PK818_06490"
     CDS             8439..>9366
                     /locus_tag="PK818_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007971726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HQY82416.1"
                     /translation="MTRRVLSGTAAARGSALGRARVRLPHALDVAEARIPAGEVDAEL
                     DRIHAAVETVRKEMHTLRERLHGALAHEVGEFLDLHALLLDDPELLQGIDALIRTGLY
                     TADYALRLQRDRIAAVFDGIDDPYLRSRIDDIDQVIGRLHAALHRSEADLQGVAGEIL
                     VTDNIAPTELAQLQAQGVMGVVTAAGSALSHSAILARSLHLPLVVGAAQALLKVNDGD
                     VLVIDGGTGLVVLEPDAEDLRAHRHRAEEAKRERRQLNRLRREPSRTLDGVDIRLWAN
                     AESREDVAEAHALGAAGVGLYRTEFLFMGSREI"
BASE COUNT         1458 a         3430 c         3208 g         1270 t
ORIGIN      
        1 gcaggcgcgg cacgttggtg atccgcaccg gcgcatcggc cagcagggtg gcgcacagga
       61 ccggcagcac cgcgttcttg gccccggaaa tgcgcacctc gccgtgcagg cgggcaccgc
      121 cgctgacgat gatcttgtgc atcaggcggc cgcctcgtcc ggggtcaggg tgcgcagttg
      181 cagggcgtgg atctcgcccc ccatcagcgc cccgagggtg gcgtagacca tgcgatggcg
      241 cgccagcggc aacttgccgg cgaatgcggc gcacaccact tcggcctcga aatgcacgcc
      301 gtccgcgccc tggaccgcga cgcgggcgcc gggcaggccg gcttcgatca gtttctggat
      361 ggtgtcggcg ttcatgggca ggctgctgga ctcgcgtaaa ctgaacgacc aagtctagcg
      421 gatgcctcct tggaaaagcc gcccgtgccc cctgccctgc cggccagcga tttcgacttc
      481 gccaccagcg ggcggcgcgt gttcgcggtc gagcgcgatg gcctggccgc cgtcgccgcg
      541 cgcatcgatg gcgccttcag cagtgcctgc acggcgatcc tcggcggccg cggccgggtg
      601 gtctgcaccg gcatgggcaa gtccggccat gtcgcccgca agatcgccgc gaccctggcc
      661 tccaccggca ccccggcgtt ctacatgcac ccgggcgaag ccgcgcacgg cgacctcggc
      721 atggtcaccg acgccgacat cgtgctggcg ctgtcctatt ccggcgaaag cgacgagttg
      781 ctgctgctgt tgccggcgct caggcgccag ggcaacttcg tgatcgcgat gaccggccgc
      841 gggctttcca ccctcgcccg cgaagccgac ctgcacctcg acgtcagcgt gccggccgaa
      901 gcctgcccgc tggcgctggc gccgacctcc agcaccaccg ccgcgctggc gatgggcgat
      961 gcgctggcgg tggcgctgct cgaggcccgc gggttcaccg ccgacgactt cgcgcgctcg
     1021 cacccggccg gcgcgcttgg ccgccgcctg ctgctgcaca tcaccgacgt gatgcacgcc
     1081 ggcgacgagg tcccgcggat cggcccgggc gccagcatca acgaggcgct ggtggaaatg
     1141 agccgcaagc ggctggggat gaccgcggtg gtcgacgacg ccggccgcct gctcggcctg
     1201 tacaccgacg gcgacctgcg ccgcagcctg gatgacgccg cggtcgacct gcgcaccacc
     1261 cgcatcgacg cggtgatgac gcgcgcgccg cgcaccatcg acgccgacgc gctggcggtg
     1321 gaagccgcgc agctgatgga ggcgcacaag atcaacgcac tgctggtggt ggacggcgac
     1381 ggccgcgtgg tcggcgccct caacatccac gacctgctgc gcgcacgggt ggtgtgatgg
     1441 cgatgcccca ctacccggac gcgctgctgc aacgcgcggc gaagatcagg ctggtcggct
     1501 tcgacgtcga tggcaccctc accgacggcc gcctgcacta cgccagcgat ggccacgaaa
     1561 ccaagtcctt ccatgtccag gacggcatgg ggctggtgct gctgcgcgag gccgggatcg
     1621 aactggcgct ggtcagcgcg cggatcagcc cgctggtggc ggcgcgcggc agcgagttga
     1681 agatcgccca cgtccacgtc ggcgagcgca gcaagctggc ctgcatgcag cggatcgccg
     1741 ccgggatggg gatcggcatg gacgcggtcg cgttcatggg cgacgacctg ccggacctgg
     1801 ccaccctgcg cgcggccggg ctggcgatcg cgccggcgaa cgcgcacccg tgggcgctgg
     1861 cggccgcgca ctgggtggcc ccgcgcaacg gcggcgaggg cgcagcgcgc gacgcctgcg
     1921 acctgatcct gcaggcgcag ggcaggctcg agggcatcct tgcgcacggg gaacatccgt
     1981 gagctggcgc ggcggcctga ccgtggtcct gctgctggcc gcgatcgcct gcggctggtc
     2041 ggtctggcgg ttcacgggcg gtggtgccga agccatggca tcgacccgct ccgagtacgt
     2101 gctgcacgat tacgaaatcg tctcgctcga ttccgaaggc aaggaatcgt tcaccctcag
     2161 cggcccacgc ctgcagcgcg acccgggcgc gaagtcgatg agcctggaaa ccccgcgctt
     2221 cctggtgccc gaccgcaatg gcgcgtactg ggacgtgcgc gcgcaacgcg ggctggtgcc
     2281 ggacgacggc aaggaactgc ggctgcgcgg aagggtggtc gccaccagcc cgccgcaggc
     2341 accgccgccg acccggatcg aaaccgagga gctgaacctg ttcccgcgcg agaaccgcgc
     2401 ggcctcggcc agcacggtga ccctcacccg acccgggctt acaatgcgcg gccacggcat
     2461 gcgggcggat ttcgaccgcc agcaggtctc cctcctttcg gatgtgcaca cccgctatgt
     2521 cccgacgccc cgctgacctc gcggccgccg tgctcgccct gtccgccgcc gccttcgcgg
     2581 ccggcgcggg cgcgcgcagc tccgaccgca accagccgat ggacatcgag gcggcgcaca
     2641 gcacctgcag cctgggtgcc aacgacagct gcaccctgac cggcaacgtg accatgaccc
     2701 agggcagcct gaacgtggtc gcggccaagg cgatcatcga acagagcggc ggcgacccca
     2761 gccgcgccct gctcagcggc ggcgtgaccc tgcgccagac cctggacgac ggcaaccaga
     2821 tcgacgccac cgcaagcaac gtggactaca acctcaagac cgaggtggtg gtgttcaccg
     2881 gcaacgtcga gatccgccag ctgcgcggca ccctcaacgg ccagcgcgtg gtctacaaca
     2941 tgaagaccaa ccaggtcgag agcggcggca ccggcaacgg ccgggtcaag atgcggatcc
     3001 tgcccaagag cgcgcaggcg cagcccgcga aggccgcggc cgcgccggtg gtgccggccc
     3061 cgccggccgg caagcgctga tgctggccgc cagcggcctg cgcaagcgtt acaagtcgcg
     3121 cgaggtcgtg cgcgacttcg ggctgaccct ggatgcgggc gaagtggtcg gcctgctcgg
     3181 cccgaacggc gccggcaaga ccacctgctt ctacatgatc gtcggcctgg tgccggcgga
     3241 tgccggcagc atcgtgctgg acggcaccga catcaccggc gagccgatgt acgcacgcgc
     3301 caagcgcggc gtcggctacc tgccgcagga accctcggtg ttccgcaagc tcagcgtggc
     3361 ggacaacatc cgcctggtgc tggagctgcg cgacgacctc gacgacgacg gccgcgcgcg
     3421 cgaactggaa agcctgctcg acgagttgca gatcggccac gtcgccgagc agatgggcgc
     3481 cagcctgtcc ggtggcgagc gccgccgggt cgagatcgcg cgcgcgctgg ccggcaagcc
     3541 gcggatgatc ctgctggacg aacccttcgc cggcgtcgac ccgatctcgg tcaacgagat
     3601 ccagcgcatc gtccgccacc tcaaggaccg cgggatcggc gtgctgatca ccgaccacaa
     3661 cgtccgcgag accctcggca tctgcgaccg cgcctacatc ctggccgatg gcgcggtgct
     3721 cgcgcagggt gccccggatg ccttgctgga gaacccggac gtgcgccggg tctacctggg
     3781 cgacgcgttc aagctctgac cgccagggca cggccacgtc cgccccggct ttcgcgcgcc
     3841 gctaacgctt tttgcccgcg ccaggatata tcctgagcac ggatgaagcc gcgcctctcc
     3901 cccgggctgc accagcaact gatcctgacg ccgcagctgc gccaggcgat ccggctgttg
     3961 caactgtcca ccgtggaact ggaagccgaa gtcgccgagg cggtggccag caacccgctg
     4021 ctggactggg ccgaggaagg cgagccgggt cccgccgatg cccacgaggt gcgcgatgcc
     4081 gacggcatcc aggaagcgcc ggtgcgcgag cacaccgagg aacgcagcga cgagcactgg
     4141 gatcgcgaag gcgaaccctg gcaggagcgg atcggccccg catcggatcc gggcgacgat
     4201 gatttcggcg gcgccggcga acgcgcggcg agcgcggaga ccctgcgcga ccacctgctt
     4261 tggcaattgc acctcggcca cttcagccag cgcgaccagg gcatcggcgt ggcgctggtc
     4321 gatgcgatcg acgaggacgg ctacctgcgc gacgacctcg aggccatcgc cgccgggctg
     4381 cggcccgaga tcgatgccgg cctcgacgac gtgctgcagg tgctgcaccg gatccagcag
     4441 ttcgacccgg tcggggttgg cgcccgcgac ctcggcgaat gcctgcgcct gcagctgcgc
     4501 gcgctcgccg aagacacccc cgggcgcgag ctggcgatgg cggtgaccgc cggcgcgctg
     4561 gcgcggttgc cacggatcgg gatcgacggc ctgtgccagg aactggactg cacgcgcgac
     4621 caggcagagg cggcggtgca cctgctgcgc agcctggatc cgcgcccggg cgcccagcat
     4681 ggcgagcttc cggccggtac ctacgtcatc cccgattgcg tgatctggcg ccagcagggc
     4741 ctgtggcggg tggcgcttgc cgccggcgcg accccgcgga tcaccatcca gcgcgactac
     4801 gagcagatga tccggcacgc ggcggcctcc gatgccgact acctgcgcgg ccgcctgcag
     4861 gaagcgcgct ggctgctcaa gggcctggag gcgcgcgccg ataccttgct gcgggtggtc
     4921 cgctgcctgg tccgcgagca ggccggcttc ctcgagttcg gcgaccaggc cctgcatccg
     4981 ctgaccctgc gcacggtggc ggcggaactc ggcctgcacg aatccacggt gtcgcgcgcg
     5041 atcgcgcgca agtacgtgcg cacaccgcgc ggcaccatcc cgctgcgcga tttcttcgcc
     5101 tccgggatcg acaccgggaa cggcggaagc gcgtccagca ccgccatcca ggacatgatc
     5161 cgcaagctgg tcgaggccga ggacccgcgc aagccgctgt ccgacgcgcg cctcgccgac
     5221 accctgaaat cgggcggggt cccggtggcg cggcgtaccg tcgccaagta tcgtgaagcc
     5281 ctgcaaatcc ccgcatcgca cgaacgcgtg cgcatcggct gaccgatgcc acgcgccgcg
     5341 attcgttcca cacctgtcgt gtccacgtcg aaggaggtcc agatgcagat cgaaacccat
     5401 ggccagcaac tcgaagtcac cccggccctg cgcgactacg tggaaacccg cttcgagcga
     5461 ctcgggcggc acttcgagca cccctgccag atccgcgtgc agctcggcct ggacaagccc
     5521 gaccacaagg ccgaggcgac cgtgagcctg tccggccgca ccctgcacgc cgacgccaac
     5581 gcccaggaca tgtacgccgc catcgacctg ctcgccgaca agctggaccg cctgctgctg
     5641 aagcagaagg aacggatggt cgaccaccac cgcggcgagg gcatcgcgca cgtcggcacc
     5701 gcggattgac cgccttcccg ccacggcgga gccgaacccc gtgaacgccg cccgcatcac
     5761 cgccgcggaa ctgttcgacc agcaagccga acgcctggaa ctgcgctggc tggccggcca
     5821 gcgcggcggc gacgggcgca tgctggaggc ggtggagacg gttgcgcggc ggccatcgct
     5881 ggccggttac ctcaacatca tctatccgaa caaggtgcag atcctcggca ccgaggaact
     5941 ggcctggctg gacgggctgg actcgcggca gcgttgggag acgatccaga agatcatcgg
     6001 cttccgcccg ctggcgctgg tgatcagcaa gaaccagtcg tgcccggaag acctgcggat
     6061 cgccgccgag gaatcggaaa ccccgctgtg gaactcgccg cggcgcgggc acgaactgct
     6121 gaaccacctg cagtacaagc tcgcgcgcac gctggcgccg cgggtcaccc tgcatggcgt
     6181 gttcatggag atctattcga tcggcgtgct gatcaccggc gagtcgggtt ccggcaagag
     6241 cgaactggcg ctggaactgg tgacccgcgg ccaccggctg gtcgccgacg acgcgcccga
     6301 gttcacccag atcgcgcccg acgtgctcga cggcgcctgc ccggacctgc tgcaggacat
     6361 gctggaactg cgcggccttg gcgtgctcaa catccgccag atgttcggcg acaccgcggt
     6421 caagcggaac aagtacctgc gcatgatcgt gcacctgagc aagccctcgc tggaggccaa
     6481 gcccggcggc atggagcggc tcaccgggga catcggcagc cgccgcctgc tcgacctcga
     6541 cgtgccgatg atcaccctgc cggtgatggc cggccgcaac ctcgcggtgc tcaccgaggc
     6601 cgccacccgc atgcacctgc tgcgctccaa gggcgtcgac cccgccgccg cgttcatggc
     6661 ccgccacagc catttcctgg agcgctcgca gtgaccaccg cggatcccgg ccccaccccg
     6721 caggcgctgc caccgctggt gatcgtcagc ggcatgtccg gctccggcaa gtcggtggcg
     6781 ctcaacacct tcgaagacct cggctactac tgcgtcgaca acctgccggc ggaactgctg
     6841 cccgaattcg tgcgccggat cctggcccgc gacaacgcgc cggagaagat cgcggtcggc
     6901 atcgacgcgc gcagcctcgg cgacctgtcg gaactgcccg aggcgctttc gcaggtcggt
     6961 gcgcaaggcc tcgatacccg cctgctgttc ttcgataccc gcgacgacgt gctgctgcgc
     7021 cgctacgcgg atacccgccg ccgacacccg ctcagccacc tcgggctggt gctggcggac
     7081 gcgatctcgc tggagcgcca ggcgctcaag ccgctgcggc agatcgccga cagccagctg
     7141 gacaccagcg acctcaatgt ccaccagatg cgccggttga tcctgaccga atacgcgctc
     7201 agcggcgccg ggctgtcgtt gctgttcgaa tccttcgcgt accggcgtgg cgttccggcg
     7261 gaagcggact tcgtgttcga tgcgcgggtg ctgcccaacc cgcactggga cgcccgcctg
     7321 cggccgctgt ccgggcgcga tgcggacgtg cgcgactacc tcgaggcgca gccgcaagtg
     7381 gccgaatacg tcggccagat ccaggggttc ctggacacct ggctgccacg gctgaagtcg
     7441 gaaacccgca gctacgtgac ggtggccttc ggctgcaccg gcggacgcca ccgatcggtg
     7501 tacctggccg aacgccttgc ccgccattgc cgcgaagccg gctgggcgga agtggcggtg
     7561 catcaccgcg agctggactg accgcctgcc tttcgccatt cgcgcaatcg ttcgctggcg
     7621 cctgccgccg ggggttcgcg cagcggcatt tcgaggcgcg tagcggactc ccggcggcag
     7681 gcgccagcga acgcttcccg cttccgcact gtcaccaccg tctgttaact tgcaggcatg
     7741 gccgtcggca tcctgctcat cacccacgaa ggcatcggca acgcgctggt cgcggtcgcc
     7801 acgcgcctgc tgcgcaagct gccgcttgaa accgaagcgt tcgaggtgcc gttcgacggc
     7861 gacctcgacg cgctgctgcc gcttgccagc gcggccctgc gccgggtcga cggcggccat
     7921 ggcgtgctgg tgctgaccga cctgtacggc gccaccccca gcaacctcgc cgccaggctc
     7981 gcgcgcctgg gcaccccggt gcgccgggtg tccgcactga gcctgccgat gctgctgcgg
     8041 gtgatgaact acgccgacct gggcctggac gaactgccgg cggtggccgc cgcgggcgcg
     8101 cgcaacgggg cgatcaccga tgatgcctga gccaacgatg ccccagccga cccacgcgag
     8161 gacgttgccg tgatccagcg cgacctgacc atcatcaacc gcctcggcct gcacgcgcgc
     8221 gccaccgcca agctggtgca gctgctgtcc ggcttccgct gccaggccac gctggaagcg
     8281 cgcggccgcg aagtgaacgc caagagcatc atgggggtga tgctgcttgc cgccggcacc
     8341 ggctccaccg tggtgctgcg ggtcgatggc gaggacgaac aggctgccgc ggacgcggtc
     8401 gccgcactgt tcgagcgccg cttcgacgag gacagctgat gacccggcgc gtcctgtccg
     8461 gcaccgccgc cgcccgcggc agcgcgctcg gacgggcacg cgtccgcctg ccgcatgccc
     8521 tcgacgtcgc cgaggcccgg atcccggcag gcgaagtcga tgccgagctc gaccgcatcc
     8581 atgccgcggt cgaaaccgtg cgcaaggaaa tgcataccct gcgcgagcgc ctgcacggcg
     8641 cgctcgcgca cgaagtcggc gagttcctcg acctgcacgc gctgctgctc gacgaccccg
     8701 agctgctgca gggcatcgac gccctgatcc gcaccggcct gtacaccgcc gactacgccc
     8761 tgcgcctgca gcgcgaccgc atcgccgccg tgttcgacgg catcgacgat ccctacctgc
     8821 gcagccgcat cgacgacatc gaccaggtga tcggccgcct gcatgccgcc ctgcaccgca
     8881 gcgaagcgga cctgcagggc gtcgccggcg agatcctggt caccgacaac atcgccccca
     8941 ccgaactggc gcagttgcag gcgcagggcg tgatgggcgt ggtcaccgcc gccggcagcg
     9001 cgctctcgca cagcgcgatc ctcgcccgca gcctgcacct gccgctggtg gtcggcgccg
     9061 cgcaggcgct gctgaaggtc aacgacggcg acgtgctggt gatcgacggc ggcaccggcc
     9121 tggtcgtgct ggaaccggat gccgaggacc tgcgcgcgca tcgacatcgc gccgaggaag
     9181 ccaagcgcga gcgccgccag ctcaaccgcc tgcggcgcga gcccagccgc accctggacg
     9241 gtgtcgacat ccgcctgtgg gcgaatgcgg aatcgcgcga ggacgtcgcc gaggcgcatg
     9301 cgctgggtgc cgccggggtc ggcctgtacc gcaccgaatt cctgttcatg ggcagccgcg
     9361 agatcc
//
LOCUS       DAORUK010000076         9082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_8285_length_9082_cov_18.269192, whole genome shotgun sequence.
ACCESSION   DAORUK010000076 DAORUK010000000
VERSION     DAORUK010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 9082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9082
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8285_length_9082_cov_18.269192"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..543)
                     /locus_tag="PK818_06495"
     CDS             complement(151..543)
                     /locus_tag="PK818_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HQY82417.1"
                     /translation="MSTAYWCILIAAALPYAWVAIAKSAVPGYNNKDPRGWIARQDNY
                     RVRNAFAAHQNALEAFPAFAAAVLMAQLAEVDPQRVTWLAMAFIGLRILHGVFYLAGS
                     HALRSLSWFGGIACVIALMASAASRLAG"
     gene            complement(648..2378)
                     /locus_tag="PK818_06500"
     CDS             complement(648..2378)
                     /locus_tag="PK818_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014035712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQY82418.1"
                     /translation="MRHAPALFRTLPLALLALCACTPGGDRQSATSETAAPAEAATVA
                     SGHAFNDAIDAGDFAEHVQQLASDAFGGRGPGTPGEDKTVDYLKAQFARIGLQPGNGG
                     DWFQTVPMVETTADEATALNLTVAGAPQVLKFGSDMVVGTRTGQAQVSIKDSPLVFVG
                     YGVDAPEQQWNDYAGIDVKGKTVVMLVNDPGFHANDARLFDGKRMTYYGRWTYKFEEA
                     ARKGAAAALIIHDTEGASYGWDVVRNSWSGPQYDLPAKDDPAPRVPAQGWITGEAASA
                     LFKAAGQDLAKLRSAANKRGFKPVPLDATLSMELASRSVEKSSRNVIGLLPGSETPDE
                     AIVYMAHWDHLGTHEGEAGDNIYNGAVDNATGVAAILELAEAFRKSKTPPKRSILFLA
                     VTLEESGLLGSKYYVAHPVVPLDKTVAVINLDALAPVGKARDITVVGQGSSELESVLE
                     TVLDSQQRKAVGETNTAAGYYFRSDHFNFAKAGVPALYVDSGTDLLEGGQAAGDAAGK
                     DYTDNRYHKPGDQYDAATWRLDGIVQDVETVYAVGDVLVNGDAWPNWNAGNPFKAARD
                     KQRPAAPASP"
     gene            complement(2445..3134)
                     /locus_tag="PK818_06505"
     CDS             complement(2445..3134)
                     /locus_tag="PK818_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82419.1"
                     /translation="MNRTLFFIAMLATAGIAHGHDSTAGDGRGRSIDKVNGGITAQAG
                     QSYGDLSTVNGGIDLERGASAGEVETVNGGIDAGDDVATRSVATVNGGIRFGQRARID
                     GGVETVNGGIFIDRGGNVRHDVSTVNGAIGLVDTDLGGGIETVNGDVTVGAGSHVQGG
                     IHFEKPGGKWGTRWGKPKPPRVVIGPGAVVEGDLAFEREVTLYVHQSARIGHVSGATA
                     RRFETPTAPKD"
     gene            3293..4831
                     /gene="gpmI"
                     /locus_tag="PK818_06510"
     CDS             3293..4831
                     /gene="gpmI"
                     /locus_tag="PK818_06510"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HQY82420.1"
                     /translation="MPQSPRPKPVVLLILDGWGHREEVADNALALAELPNWRRLLATR
                     PHTLVHTEGRHVGLPDGQMGNSEVGHMNLGAGRIVYQDLTRVDAAIEDGSFFANAELR
                     AACSAAKAANATLHVIGLLSPGGVHSHQAHIFAMLELAHREAVPRVAVHALLDGRDTP
                     PRSAEASLRALQDLCGRLGNARIASIGGRYYAMDRDKRWERVRRGWDAIVEAIGDHAA
                     ADALSALQAAYARGENDEFVAPTVLAETQPMGDGDAVVFMNFRADRARQLAAAFVEPA
                     FDGFAARRPALSRFVCLAEYDAKLPASVAFAPDDLRHTFGEVLADAGLAQLRIAETEK
                     YAHVTFFFSGGREDPFAGESRILVPSPQVATYDLQPEMSCPELTDRLVAAIESQAFDA
                     IVCNIANPDMVGHTGILDAAILAAQAVDVAIGRIDEAVRRAGGALLVTADHGNLEMMR
                     DPQTGQPHTAHTVGPVPLVYVGGRPAALRGGGALRDIAPTLLDLLGLPQPAEMSGRSL
                     LLPA"
     gene            4831..6039
                     /locus_tag="PK818_06515"
     CDS             4831..6039
                     /locus_tag="PK818_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQY82421.1"
                     /translation="MRTLRAIVLLALAMAAAAAQAQDPREAEQRLQKVRSELKAVASE
                     RRKLEGARGDATRQLRQADEQVGSVQRNLRGIEATLQRDSETLAQLQAERATHAGDLD
                     ASKAELARLLRAAQLAGADAPLKAMLAQDRIAEAERALAYQGYLQRGRVQRIRELGAE
                     LARIDALEREIGQRQAALAQDREQHAAQLAQLALARKQRAGVLAGIDRQYQDKAGREQ
                     ALGRDAKALQGLLAQLRAAAAKAAREAAQAKAAAERAAKASGKPVPKRTPVASAPAMK
                     VGGLGWPVAGSLLAAYGGALPDGRRSDGVLIAAPAGSTVKAVADGTVVFADWMTGYGN
                     ILIIDHGNGYMSLYAHNDGLLREAGDAVRRGDAVASVGTSGGQGRPALYFELRRNGAP
                     VNPGTWLTRQ"
     gene            6137..7516
                     /locus_tag="PK818_06520"
                     /pseudo
     CDS             6137..7516
                     /locus_tag="PK818_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007968182.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
     gene            complement(7513..8205)
                     /locus_tag="PK818_06525"
     CDS             complement(7513..8205)
                     /locus_tag="PK818_06525"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQY82422.1"
                     /translation="MFVGISTRRKTVFRWAAPLLFVALWLAYVLGAMRAPALRQGAEL
                     DWGVLSGGLDSIEAWRQALVDGSVLRLASALFLHADWIHLLGNLVFLLIFGLPAERVM
                     GGWRFLVLFLLGGAVANLAAVLAIGAPDRVIIGASGAVSAVIGAYLALFPGARLGVVL
                     PLGLFLEFVRVPASLLIGLWALLQLVFAYSGPAFGAVAWPAHIAGFLFGVVFALFARA
                     AIARRLRQSRGY"
     gene            8403..>9082
                     /locus_tag="PK818_06530"
     CDS             8403..>9082
                     /locus_tag="PK818_06530"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HQY82423.1"
                     /translation="MKTTTRSFRATALALAVLGTLAAGQASASGFQLREQSVKNLGKS
                     NAGSIVGKDASNVSLNPAAMTNLDKVTIQSDLTVIDLSASFSGGGSILGSPAPSAALT
                     GGNGGDPGDPTLVPNMAVVFPMHGALEGMTLGASIGAPFGLKTEYEPGWVGRYRALTS
                     DVKVVDLTLSAAFKATDGVSFGAGLVYERADVTLSKAIDFGTAICASGNPANCFNPQY
                     PFKPQQLDG"
BASE COUNT         1377 a         3140 c         3269 g         1296 t
ORIGIN      
        1 tttttctttg cgccacgcac cagccaaaat agcgtgcgcc ggggcgacca tgagggcgtt
       61 gcggttctat ccaagacccc agactaaacg cggcggtaga ttcttcggag cgcgcgtgcg
      121 gactgcagca gggatgaacc gtcgatcgcc ttatccggca agcctggatg cggccgaggc
      181 catcagtgca atcacgcacg cgatgccccc gaaccaggaa aggctgcgca gcgcgtggct
      241 gccggcgagg tagaagacgc catgcaggat gcgcaacccg atgaacgcca tcgccagcca
      301 cgtgacccgt tgcggatcca cctcggcgag ttgcgccatc agcaccgccg ctgcaaaggc
      361 gggaaacgct tccagggcat tctggtgcgc ggcgaacgcg ttgcgcacgc ggtagttgtc
      421 ctggcgtgcg atccagcccc gtggatcctt gttgttgtag ccgggcacgg ccgatttggc
      481 gatcgcgacc caggcgtagg gcagcgcggc ggcaatcagg atgcaccagt aagcggtcga
      541 catggcggcc ctccaggggc gctggtggag cggccagcct agcggatccc gcgcaagaag
      601 atcgggccgc ccgcgcgggc ggcaagcagt cgtggccggt gccgaattca ggggctggcg
      661 ggtgccgcgg gccgctgctt gtcacgggcg gccttgaacg gattgcccgc gttccagttg
      721 ggccacgcgt cgccgttcac caggacgtca cccaccgcgt acacggtttc gacgtcctgg
      781 acgatgccgt ccagccgcca tgtggcggcg tcgtactggt cgccgggctt gtggtaacgg
      841 ttgtcggtgt agtccttgcc ggcggcatcg cccgcggcct ggccgccttc taggaggtcg
      901 gtgccgctgt cgacgtacag cgcgggtacg ccggctttgg cgaagttgaa gtggtcggag
      961 cggaagtagt agccggccgc ggtgttggtt tcgccgaccg ccttgcgctg ctgcgaatcg
     1021 agtacggtct cgagcacgga ttccagctcc gaactgccct ggccgacgac agtgatgtcg
     1081 cgggccttgc cgaccggcgc cagcgcgtcg aggttgatca cggccacggt cttgtcgagc
     1141 gggaccaccg gatgcgcgac gtagtatttc gaaccgagca ggccggactc ctccagggtc
     1201 actgccagga acaggatcga acgcttcggc ggcgtcttcg acttgcggaa cgcttccgcc
     1261 agctcgagga tcgcggccac gccggtcgcg ttgtcgaccg caccgttgta gatgttgtcg
     1321 ccggcctcgc cctcgtgggt gccaaggtgg tcccagtgcg ccatgtacac gatcgcctcg
     1381 tccggggttt cgctgccggg caacaggccg atcacgttgc gcgaactctt ctcgacgctg
     1441 cggctggcca gttccatcga cagggtggca tccagcggca ccggcttgaa gccacgcttg
     1501 ttggcggcgc ttcgcagctt ggccaggtcc tgtcccgcgg ccttgaacag cgcggaggcg
     1561 gcctcgccgg tgatccagcc ctgcgccggt acccgtggcg cggggtcgtc cttcgccggc
     1621 aggtcgtact gcgggccgga ccaggaattc ctgaccacgt cccagccgta ggacgcgccc
     1681 tcggtgtcgt ggatgatcag cgccgcggcc gcacccttgc gcgcggcttc ctcgaacttg
     1741 taggtccagc ggccgtagta ggtcatccgc ttgccgtcga acaggcgggc gtcgttggcg
     1801 tggaagccgg gatcgttgac cagcatcacc acggtcttgc ccttgacgtc gatgccggcg
     1861 taatcgttcc actgctgctc gggcgcgtcc acgccgtagc cgacgaacac cagcgggctg
     1921 tccttgatgc tgacctgcgc ctgcccggtg cgggtgccga ccaccatgtc gctgccgaac
     1981 ttcagcacct gcggcgcgcc ggccacggtg aggttgagcg cggtcgcctc gtctgcggtg
     2041 gtctccacca tcggcacggt ctggaaccag tcgccgccat tgccgggctg caggccgatc
     2101 cgcgcgaact gcgccttcag gtagtcgacg gtcttgtcct cgcccggcgt gccgggtccg
     2161 cggccgccga atgcatccga cgcaagctgc tgcacatgct ccgcgaagtc gccggcatcg
     2221 atggcgtcat tgaaggcatg gccgctggcg acggtggcgg cttcagccgg cgccgcggtc
     2281 tcggaggtgg cggactggcg gtcgccgccc ggggtgcatg cgcacagcgc gagcagtgcg
     2341 agcggaagcg tacggaacag ggcgggcgcg tggcgcatcg gggggctccg gtggacgtcc
     2401 cccgattcta gccatgccgc cgcgcgcacg gcgtgtgccg gtggtcagtc cttcggcgcc
     2461 gtgggcgttt cgaagcggcg cgcggtcgcg ccgctgacat ggccgatgcg cgcactctgg
     2521 tgcacataca gggtcacctc gcgttcgaac gccaggtcgc cttcgaccac cgcgcccggg
     2581 ccgatcacca cccgcggcgg cttcggcttg ccccaccgcg tgccccattt gccgcccggc
     2641 ttctcgaagt ggatgccgcc ctgcacgtgc gagccggcgc caacggtgac gtcgccgttc
     2701 acggtctcga tgccgccgcc caggtcggta tcgacgaggc cgatcgcgcc gttcacggtg
     2761 gacacgtcat ggcgcacgtt gccgccgcga tcgatgaaga tgccgccgtt gacggtttcc
     2821 acgccgccgt cgatgcgtgc gcgctggccg aagcggatcc cgccgttgac cgtggcgacc
     2881 gagcgggtgg cgacatcgtc gccggcgtcg atgccgccat tgacggtttc cacctcgccg
     2941 gcgctggcgc cgcgttcgag gtcgatgccg ccgttgaccg tgctgaggtc gccgtacgac
     3001 tggccggcct gcgcggtgat cccgccattg accttgtcga tgctgcggcc gcggccatcg
     3061 ccagcggtgg aatcgtggcc gtgggcgatg ccggcggtgg cgagcatggc gatgaagaac
     3121 agtgtgcggt tcatgcggga atcctcggat ggctgcagac tgggatgccg gcaatgcgcg
     3181 cagggtttac atcgaccacg ccttgccgcc gccgcgccgg tccgccgccg ggggcttggc
     3241 tacactatcg gcggcccgga tcgcgggccg ccgccacccc caggagccgc acgtgccgca
     3301 gtccccgcgc ccgaaacccg tcgtgttgct gatcctggac ggatggggcc accgcgagga
     3361 ggtggccgac aacgcgctgg cgctggccga gctgccgaac tggcggcgcc tgctggcgac
     3421 ccggccgcac acgctggtgc ataccgaggg ccgccacgtc ggcctgccgg acgggcagat
     3481 gggcaattcc gaagtcggcc acatgaacct cggtgccggc cgcatcgtct accaggacct
     3541 gacccgggtc gacgccgcga tcgaggacgg cagcttcttc gccaacgccg agctgcgcgc
     3601 cgcctgctcc gcggcaaagg ccgccaacgc caccctgcac gtgatcggcc tgctgtcgcc
     3661 gggtggcgtg cacagccacc aggcgcacat cttcgcgatg ctcgaactgg cgcatcgcga
     3721 agcggtgccg cgggtcgcgg tgcatgcatt gctcgacggc cgcgataccc cgccgcgctc
     3781 cgccgaagcc agcctgcgcg cgctccagga cctgtgcggg cggcttggca acgcgcgcat
     3841 cgccagcatc ggcggccgct attacgcgat ggaccgcgac aagcgctggg agcgggtcag
     3901 gcgcgggtgg gacgcgatcg tcgaggccat cggcgaccac gccgccgccg atgcactgtc
     3961 ggccttgcag gcggcgtacg cacgcggcga gaacgacgaa ttcgtggcgc cgacggtgct
     4021 cgccgaaacg caaccgatgg gcgatggcga cgcggtggtg ttcatgaact tccgcgccga
     4081 ccgtgcacgg caactggccg cggcgttcgt ggagccggcg ttcgacggtt tcgccgcgcg
     4141 ccgccccgcg ctgtcgcgct tcgtctgcct ggccgaatac gacgcgaaac tgccggcttc
     4201 ggtggcattc gcgcctgacg acctgcgcca caccttcggc gaggtgctgg ccgatgccgg
     4261 gcttgcccag ctgcggatcg ccgagaccga gaagtacgcg cacgtcacct tcttcttcag
     4321 tggcgggcgc gaggatccgt tcgccggcga gtcgcggatc ctggtgccca gcccgcaggt
     4381 cgcgacctac gacctgcagc cggaaatgag ctgcccggag ctcaccgacc ggctggtcgc
     4441 ggcgatcgag tcgcaggcct tcgatgcgat cgtctgcaac atcgccaacc cggacatggt
     4501 tggccacacc ggcatcctcg acgccgcgat cctcgccgcg caggcggtgg acgtggccat
     4561 cggccggatc gacgaggcgg tgcggcgcgc cggcggcgcg ctgctggtga ccgccgacca
     4621 cggcaacctg gaaatgatgc gcgacccgca gaccggccag ccgcataccg cgcacacggt
     4681 cggcccggtg ccgctggtgt acgtgggcgg gcgcccggcg gcgttgcgcg gtggtggtgc
     4741 gttgcgcgac atcgcaccga ccctgctcga cctgctgggg ctgccgcagc cggcggaaat
     4801 gagcgggcgc agcctgttgc tgccggcctg atgcgcacac ttcgcgcgat cgtgctgctg
     4861 gcgctggcga tggccgccgc cgccgcgcag gcgcaggacc cgcgcgaggc cgagcaacgc
     4921 ctgcagaagg tgcgtagcga actgaaggcg gtcgccagcg aacgccgcaa gctggaaggc
     4981 gcgcgtggcg atgccacccg ccagttgcgg caggccgacg aacaggtcgg cagcgtgcag
     5041 cgcaacctgc gcggcatcga ggccaccctg cagcgcgaca gcgagacact ggcgcagttg
     5101 caggccgagc gcgcgacgca tgcgggcgac ctcgatgcca gcaaggccga gcttgcgcgc
     5161 ctgctgcgcg cggcgcagct tgccggtgcc gatgcgccgt tgaaggcgat gctggcgcag
     5221 gaccgcatcg ccgaagccga acgcgcgctg gcctaccagg gctacctgca gcgcgggcgc
     5281 gtccagcgga tccgcgaact gggcgctgag ctggcgcgca tcgatgcgct ggaacgcgag
     5341 atcggccaac ggcaggcggc gctggcgcag gatcgcgagc agcacgcggc ccagcttgcg
     5401 cagctcgcgc ttgcgcgcaa gcagcgcgcc ggcgtgcttg ccgggatcga ccgccagtac
     5461 caggacaagg ccggccgcga gcaggccctg gggcgcgatg ccaaggccct gcaggggctg
     5521 ctggcgcagt tgcgcgcggc ggcggcgaag gcggcacgcg aggccgcgca agcgaaggca
     5581 gcggcggagc gcgcggcgaa ggcatcgggc aagccggtgc cgaagcgcac gccggtggcc
     5641 agcgcgccgg ccatgaaggt gggtggcctg ggctggccgg tggccggcag cctgctggcc
     5701 gcgtacggcg gggcgttgcc ggacgggcgc cgcagcgatg gcgtgctgat cgccgcgccc
     5761 gccggttcca cggtcaaggc ggtggcggac ggcacggtgg tgttcgcgga ctggatgacc
     5821 ggctacggca acatcctgat catcgaccac ggcaacggct acatgagcct gtacgcgcac
     5881 aacgacggcc tgctgcgtga agccggcgat gcggtcaggc gcggcgacgc ggtggccagc
     5941 gtcggcacct ccggtggaca gggacggccg gcgctgtatt tcgagttgcg gcggaacggc
     6001 gcgccggtca acccgggcac ctggctgacc cgccagtagc gccggcaggc gccatcgcga
     6061 cgcgccggcc gggcatgatg gcggaacccg gcgccagccg cggggtcgaa ccgacatcca
     6121 tcgagcggag caggacatgc gggccaaacc actttcgttg ttgatcgccg gcttcctgtg
     6181 gatggcgccg tgggtcgcgc ccgcgcagga cgcgcggcaa ccggatccgc gcgccgccag
     6241 cgaggaggcc gccgtgccgg tggccgccag cgacgacccg gatgcggccg acaccgccgc
     6301 cgcgaaagtg ccgctggacg agatccgccg ctacgtgatg gtctacaacg cggtcaagca
     6361 ggcctacgtg gacccggtcg acgaccatgc gctgatgcag tccgcggtgc gcggactgtt
     6421 gctcgacctc gacccgcaca gtgcctacct gcgcaaggag gatgcggatg acttcaatga
     6481 acagacccgg ggcgcgtatg acggcgtcgg gctcgagttg cagcaattgc cggagggtgg
     6541 gctgcggatc gtcgccccgc tcgacgatgg ccccgccgag cgcgccggcc tgcgcagcgg
     6601 cgaccgcatc ctcgcgatcg acggcaagct gctgaccccg gcggacgcgg atgtctcggc
     6661 gccattgcgc ggtccggccg gcaccagggt ggtgttgcgg atcgcgcgcg aaggcacgcc
     6721 gcagccgttc gaggtgccgc tggtgcgcga gaccatccgc accaccagcg tgcgctcgcg
     6781 gatgctggag cccggcttcg gctatgtccg ggtcggcagt ttccaggcgg cgaccgcgaa
     6841 cgacttcgcc aaggcgctcg atgccctgca gaaggatgcg ccgctgcggg gcctgctgct
     6901 cgacctgcgc agcaatcccg gcggcctgct ggtgggcgcg gtgcagatcg ccgacgagct
     6961 gctcgacaag ggcgggatcg tgagtacccg cggccgcgcg ctgatcggcg acagccgttt
     7021 cgacgcgacc cccggcgacc gcctcaaggg ggcgccggtg gtggtgctgg tcgatgccgg
     7081 ttcggccagc gcggccgaag tgctggccgg tgcactgctc gacaaccggc gcgcacgggt
     7141 ggtcggcagc cgcaccttcg gcaagggctc ggtgcagacc ctgttgccgc tggacaacgg
     7201 cgattcggtc aagctgacca ccgcgcgcta ttacaccccg ggcgggcatt cgatccaggc
     7261 gcgcgggatc gtgccggacg tggtgctcag gccggaggcc ggcaccgggc gcggggcgcc
     7321 gatcagcgaa gcggcgctgc ccgggcacct gcagggcgag ggcgaggcaa tggagggcga
     7381 caacgcaggc gaggtgttgc ccggcccgaa gccggtcgat gccgcgttgc tggaactgaa
     7441 gaagatggcg gggggcgtgg cgcggccggc gacagcagcg ccgccagccg ccgcccggcc
     7501 gcacgcgaag gctcaataac cacggctctg gcggagcctg cgggcgatcg cggcgcgggc
     7561 gaacagggca aacaccaccc cgaacaggaa accggcgata tgcgccggcc acgccaccgc
     7621 gccgaacgcc gggccgctgt aggcgaacac cagctgcagc agcgcccaca ggccgatcag
     7681 cagtgatgcc ggcacccgca cgaattccag gaacaggcca agtggcagca ccacccccag
     7741 gcgtgcgccc gggaacagcg cgaggtaggc accgatcacc gcggagaccg cgccgctggc
     7801 gccgatgatg acccggtccg gtgcaccgat ggccagcacc gccgcgaggt tggccaccgc
     7861 gccgccgagc aggaacagga ccaggaaccg ccagcccccc atcacccgct ccgccggcag
     7921 cccgaagatc agcaggaaca ccaggttgcc gagcaggtgg atccagtccg cgtgcaggaa
     7981 cagcgccgag gccaggcgca gcacgctgcc gtcgaccagg gcctggcgcc aggcctcgat
     8041 gctgtcgagg ccgcccgaga gcacgcccca gtcgagttcc gcaccttgcc gcagcgccgg
     8101 cgcgcgcatc gcgcccagca cataggccag ccagagcgcc acgaacagca acggggcggc
     8161 ccagcggaac acggtcttcc tgcgggtgga tatgccgacg aacatgggct gccactgtac
     8221 cgactgcggc ctggtttgtt agcttcgggt taatttacgc gctatattgc atacggcgac
     8281 gtacgtcggg ggagggctgc tgctgcagag tcccggcggg cgccgcgcgc acaagcgctc
     8341 cgatgcgcct gcatcgcgcc cgactaaaac aatccctgca aagagtgacg gagacgattc
     8401 gaatgaagac caccacccgc agcttccgcg ccaccgcgct cgcgttggcc gtgctcggca
     8461 ccctggccgc cggccaggcg tccgcgtccg gcttccagct gcgcgagcag agcgtgaaga
     8521 acctcggcaa gtccaatgcc ggcagcatcg tcggcaagga tgcgtcgaac gtgtcgctga
     8581 acccggcggc gatgaccaat ctcgacaagg tcacgatcca gagcgacctc accgtgatcg
     8641 acctgtccgc cagcttcagc ggcggtggca gcatcctcgg ctcgccggcg ccgtccgcgg
     8701 ccctgaccgg cggcaacggc ggcgatcctg gcgacccgac cctggtcccg aacatggcgg
     8761 tggtgttccc gatgcatggc gcgctcgagg gcatgaccct gggcgccagc atcggcgcgc
     8821 cgttcggcct gaagaccgaa tacgagccgg gctgggtggg ccgctaccgc gcgctgacct
     8881 ccgacgtgaa ggtggtcgac ctgaccctgt ccgccgcgtt caaggcgacc gatggcgtgt
     8941 cgttcggcgc cggcctggtc tatgagcgtg ccgacgtcac cctcagcaag gcgatcgatt
     9001 tcggcaccgc gatctgcgca tcgggcaatc cggcgaactg cttcaacccg cagtatccgt
     9061 tcaagccgca gcagctcgac gg
//
LOCUS       DAORUK010000077         9010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_8376_length_9010_cov_17.717253, whole genome shotgun sequence.
ACCESSION   DAORUK010000077 DAORUK010000000
VERSION     DAORUK010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 9010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9010
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8376_length_9010_cov_17.717253"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..832)
                     /locus_tag="PK818_06535"
     CDS             complement(122..832)
                     /locus_tag="PK818_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019677824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY82424.1"
                     /translation="MAGNGIRIEGLRKRYGQGDTAVDALKGVDMVVAPGEVVGLIGPS
                     GSGKSTLLKCLGAVIDPSGGRMVLGDQVIYDDGWKVRDLRALRRDKIGFVFQAPYLIP
                     FLDVTDNVALLPMLAGMPNAQARAQALELLTALDVQHRAQAMPAQLSGGEQQRVAIAR
                     GLVNRPPVILADEPTAPLDSERALAVMRILDGMAKKYQTAIIVVTHDEKIIPTFKRIY
                     HIRDGVTHEEAGEGRAIA"
     gene            complement(834..2039)
                     /locus_tag="PK818_06540"
     CDS             complement(834..2039)
                     /locus_tag="PK818_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY82425.1"
                     /translation="MISLAGRDILHAWGKFVFTGIGLGLLIGVTLVMAGVYRGMVDDG
                     MALLDNSGADLWVVQKDTLGPYAESSSVNDDLYRALLGMPGVDRAANVTYLTMQVRKG
                     GQDVRAMVVGVVPGQAWGAPGWPPQLVAGRHITRSHYEAVADVASGFRPGERIRIRRN
                     EYTVVGLTRRMVSSSGDPMVFIPLKDAQEAQFLKDNDAILQGRRRSAANPAFNRPGVP
                     GLLDAVVASQAANSYVNAVLVRVRDGASADEVADAIRRWKRLTVHTRPQMEAILIGKL
                     IATSARQIGMFLAILAIVSAAIVAFIIYTLTMDKIREIAVLKLIGTRNRTIAAMIMQQ
                     ALALGVIGFAVGKITATFAAPAFPKYVLLLPGDSVTGFFAVMVICALASVVAIRMALK
                     VDPAEAIGG"
     gene            complement(2039..3187)
                     /locus_tag="PK818_06545"
     CDS             complement(2039..3187)
                     /locus_tag="PK818_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY82426.1"
                     /translation="MSWKPMQRRTLALVAVILPLLALLAYVALRSGPLAPVAVTVAEV
                     HERTIAPALFGIGTLEARHVYRIGPIFPGRLARLDVQVGDSVAAGTVLGEMAPVDIDD
                     RLRAQEALGNRAEAAIREASARQAYARGQARRYEQLYASRTVSEEAVSAKRQELAVAD
                     AALAAARQDLARARSDRDAVATQRGDLQLRAPVAGIVTLREVDPGTTVVAGQAVVELV
                     DPLSLWVNTRFDQASSHGLAAGLAADIVLRSRNGQALPGRVLRLEPKADAITEETLAK
                     VGFDSVPTPLPPIGELAEVTVALAPLAAATVIPNAAIRREGNQAGVWRLVDGDPQFVA
                     VKLGRADLDGNVQVLEGLAEGDRIVVYSAQALGPRTRIKVVERIPGVD"
     gene            complement(3184..3792)
                     /locus_tag="PK818_06550"
     CDS             complement(3184..3792)
                     /locus_tag="PK818_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQY82427.1"
                     /translation="MNSQPKYLPAEERRAVTVEAVVQLAASQNPSEISTTAIARHMGV
                     TQGALFKHFPNKDAILEAVMAWVAEHLLARVDRAAQAAASPGAAIEAMFLAHVGFVCD
                     HPGVPRMLLGELQRAQPTAPRRMAQTLLRHYRERLQRLFEAAIANGELPATLDVDAAA
                     ILLVGTVQGLVVQSLLAGDVERMRHDAPRVFAIYRRGIGGAA"
     gene            3937..4125
                     /locus_tag="PK818_06555"
     CDS             3937..4125
                     /locus_tag="PK818_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82428.1"
                     /translation="MDIHDIALNLFTGIVARQDLAATSDDSRLALGREAYLWAEAFIA
                     AKDIYIRELPVPEIDPGF"
     gene            4190..4663
                     /locus_tag="PK818_06560"
     CDS             4190..4663
                     /locus_tag="PK818_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82429.1"
                     /translation="MTPRFAWIAALLLLLAGCGSGGPVRRISEPAASLQQVSVRADGR
                     WDVQVRLDNFSSVPMRFSAATIQVALDNGAAATVQAQPQITIGPESADVFTATVTPLA
                     EGRARIATALADGRSLAYTLAGTLEAGPEDGGPRTYTIKRTSALTPVPGLPGVLR"
     gene            4761..6554
                     /locus_tag="PK818_06565"
     CDS             4761..6554
                     /locus_tag="PK818_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010378222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HQY82430.1"
                     /translation="MSSYKPPLDDIRFALFDVLGSEANFARLGFADANRELVDAVIDE
                     AARFSETVLAPLNRIGDEVGCKFDPATGDVSVPPGFKQAFDQFVEGGWTGLTNDPEFG
                     GQGMPAALGGVLTEMVDSANLAWGNFPMLSHGAVKALETYGEDWQKEVFLKPLVAGTW
                     TGTMCLTEPHCGTDLGLLKTRAIPNADDGSYAITGTKIFITAGEHDMSGNIVHLVLAK
                     LPDAPAGAKGISLFVTPKFKVARDGSIGERNAVRCGSIEHKMGIHGSATCVMNFDGAQ
                     GYLVGEPHKGLQAMFVMMNSARLGVGVQGLGLSERAYQNALRYSRERLQSRSLSGPKA
                     PDKPADPIIVHPDVRRMLLTQKALVEGGRLLSLHAYSLLDAAEHEADAGAREHADTLV
                     GFLTPISKACLTEWSIESTYNAMQCFGGHGYIHEYGMEQYARDARITTIYEGTTGIQA
                     LDLIGRKTAASQGAGLKLFLGMVEAFAKQHEGDPALAEFIAPLRAKAAEWGKLTIETL
                     QRAASNPDELGAASHDYLFYSGYVVLAYWWARSVATADASSRPQAFKDAKRETARFYF
                     ARILPRTLLHKAAIEAGAASLMAMPAEHFGE"
     gene            6736..7299
                     /locus_tag="PK818_06570"
                     /pseudo
     CDS             6736..7299
                     /locus_tag="PK818_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157096.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            7441..8535
                     /locus_tag="PK818_06575"
     CDS             7441..8535
                     /locus_tag="PK818_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82431.1"
                     /translation="MALTLGITGMDRGTEAEVQAAFKAANAETGNKWTLVAGDGADYV
                     VIDMDSLYGPMSWLRLHAAGCKVIGLTSVDRNQTDFRLPRPVSANDFAVLLSEIAGDL
                     AATPAPAAPPAASTPAPAPSDHLPPAHAVRVDHVEDAPAASVVLAPAPAEAPAAAAPE
                     PTPEAAPAATRELPPDRPLAMWLEASGLERRVRLQRGAGPVLLIDPRSGVWHGPATLK
                     PLAAYFEGTLLREDFATPEAAAWESEAAAIGAAQPLARLQWLGALLAGHGALLPGNDP
                     DGKFRLTKWPQTEREYPKHFRIATAMMKGPATIAEVAEASGVAQEEVADFVNANLATG
                     YAESARQAPPEPETPPQKPAGGLFGRMRGR"
     gene            8634..>9010
                     /locus_tag="PK818_06580"
     CDS             8634..>9010
                     /locus_tag="PK818_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HQY82432.1"
                     /translation="MIKRLGAEFIGTFWLVLGGCGSAVLAANFGGDGNPLGIGLLGVS
                     LAFGLTVLTGAYALGHVSGGHFNPAVSFGLWAGGRFQAKDLLPYILAQTIGAIFAAWI
                     LFQVASGRAGFAIDANAAGAFAT"
BASE COUNT         1387 a         3234 c         3045 g         1344 t
ORIGIN      
        1 gcaggggatt gaaaatcccc gtgtcggggg ttcgattccc tccccggcca ccatcggttc
       61 agcgagcaaa cgtcaagccc cgcaacggtt tcccgccatt gcggggtttt tcgtttgcgg
      121 ctcacgcgat cgcgcggccc tcgccggctt cctcgtgggt gacgccgtcg cggatgtggt
      181 agatgcgctt gaaggtgggg atgatcttct cgtcgtgggt gaccacgatg atcgcggtct
      241 ggtacttctt cgccatcccg tcgaggatgc gcatgactgc cagcgcgcgt tcggaatcca
      301 gcggcgcggt gggttcgtcg gccaggatca ccggcgggcg gttgaccagc ccgcgggcga
      361 tcgcgacccg ttgctgctcg ccgccggaca gctgcgccgg catcgcctgc gcgcggtgct
      421 gcacgtccaa cgcggtcagc agctccagcg cctgcgcccg cgcctgcgcg ttgggcatgc
      481 cggccagcat cggcagcaac gccacgttgt cggtgacgtc gaggaacggg atcaggtacg
      541 gggcctggaa tacgaagccg atcttgtcgc ggcgcagcgc gcgcaggtcg cgcaccttcc
      601 agccgtcgtc gtagatcacc tggtcgccca gaaccattcg cccgccgctg ggatcgatca
      661 ccgcgcccag gcatttcagc agggtgctct tgccggaacc cgaggggccg atcaggccga
      721 ccacctcgcc gggcgcgacc accatgtcga ctcccttcag tgcgtccacc gcggtgtcgc
      781 cctgcccgta gcgcttcctc aggccttcga tgcggatgcc gttgcctgcc atgtcagccc
      841 ccgatcgcct cggccggatc caccttcagc gccatccgga tcgcgaccac gctggccagc
      901 gcgcagatca ccatcaccgc gaagaagccg gtcaccgaat cccccggcag cagcagcacg
      961 tacttgggga acgccggcgc ggcgaaggtg gcggtgatct tgccgaccgc gaagccgatc
     1021 acgccgagcg ccagcgcctg ctgcatgatc atcgcggcaa tggtgcggtt gcgggtgccg
     1081 atcagcttga gcaccgcgat ctcgcggatc ttgtccatgg tcagggtgta gatgatgaag
     1141 gcgacgatcg ccgcgctgac gatcgcgagg atggccagga acatgccgat ctggcgcgcc
     1201 gaggtcgcga tcagcttgcc gatcaggatt gcctccatct gcggccgggt gtgcacggtg
     1261 agccgcttcc agcgccggat cgcatcggcg acctcgtccg ccgaggcacc gtcgcgcacc
     1321 cgcaccagca ccgcattgac gtagctgttg gccgcttgcg aggccaccac cgcatccagc
     1381 agcccgggca cgccggggcg gttgaaggcg ggattggcgg cgctgcggcg gcggccctgc
     1441 aggatcgcgt cgttgtcctt gaggaactgt gcctcctgtg cgtccttcag cgggatgaac
     1501 accatcgggt cgccgctgga ggacaccatc ctgcgggtca ggccgaccac ggtgtactcg
     1561 ttgcggcgga tgcggatgcg ctcgccgggg cggaagccgc tggccacgtc ggccaccgcc
     1621 tcgtaatggc tgcgggtgat gtggcggccg gcgaccagtt gcggcggcca gccgggtgcg
     1681 ccccatgcct gcccgggtac caccccgacc accatcgcgc gcacgtcctg cccgcccttg
     1741 cgcacctgca tggtcaggta ggtcacgttg gcggcgcggt ccacgccggg catgccgagc
     1801 agcgcccggt acaggtcgtc gttgacgctg gacgactccg cgtacggccc gagggtgtcc
     1861 ttctgcacca cccacaggtc ggcaccgctg ttgtccagca gggccatgcc gtcatcgacc
     1921 atgccgcgat agaccccggc catcaccagc gtcaccccga tcagcaggcc caggccaatg
     1981 ccggtgaaca cgaacttgcc ccaggcatgc aggatgtcgc ggccggccag gctgatcatc
     2041 agtcgacccc ggggatccgc tcgacgacct tgatccgcgt gcgcggtccc agcgcctgcg
     2101 cgctgtagac cacgatgcgg tcgccctcgg ccagtccttc cagcacctgc acgttgccgt
     2161 cgaggtcggc gcgcccgagc ttgaccgcca cgaattgcgg atcgccatcg accagccgcc
     2221 acaccccggc ctggttgccc tcgcggcgga tcgcggcatt cgggatcacc gtggccgccg
     2281 ccagcggggc cagcgccacc gtgacttcgg ccagctcgcc gatcggcggc aatggcgtgg
     2341 gcacgctgtc gaagccgacc ttggccaggg tttcctcggt gatcgcgtcc gccttcggct
     2401 ccagccgcag cacccgcccc ggcaaggcct ggccattgcg cgaacgcagc acgatgtcgg
     2461 ccgcgaggcc ggcggcgaga ccatgcgaac tggcctggtc gaagcgggtg ttgacccaga
     2521 ggctgagcgg atccaccagc tcgaccaccg cctgtccggc caccacggtg gtgcccgggt
     2581 cgacctcgcg caaggtcacg atcccggcga ccggcgcccg caactgcagg tcgccgcgct
     2641 gggtcgccac cgcgtcgcgg tcggagcgtg cgcgcgccag gtcctggcgt gccgccgcca
     2701 gcgccgcgtc ggcgaccgca agctcctggc gcttggccga gaccgcttcc tcgctgaccg
     2761 tgcgcgatgc atacaactgt tcgtagcgcc gggcctggcc acgcgcatac gcctggcgtg
     2821 cgctcgcttc gcggatcgcc gcctcggcgc ggttgcccag cgcttcctgc gcgcgcaggc
     2881 ggtcgtcgat atccaccggc gccatctcgc ccagcacggt gccggcggcg acgctgtcgc
     2941 cgacctgcac gtccagccgc gccagccggc cggggaagat cggcccgatc cggtacacat
     3001 ggcgtgcttc cagcgtaccg atgccgaaca gcgccggcgc gatcgtgcgc tcatgcacct
     3061 ccgccacggt gaccgcgacc ggcgccagcg gcccggaacg cagtgccacg taggcgagca
     3121 aggccagcag cggcaggatc accgcgacca gcgccagggt gcgtcgctgc atcggtttcc
     3181 agctcatgcc gcacccccga tcccgcgacg gtagatcgcg aacacccgtg gcgcgtcgtg
     3241 gcgcatgcgt tcgacatcgc cggccagcag cgattgcacc accagcccct gcacggtgcc
     3301 gaccagaagg atggccgcgg cgtcgacatc cagcgtggcc ggcagctcgc cgttggcgat
     3361 cgccgcttcg aacaggcgct gcaggcgctc gcggtaatgc cgcagcaggg tctgcgccat
     3421 tcgcctgggt gcagtcggct gcgcccgctg caactcgccg agcagcatcc gcggcacccc
     3481 cgggtggtcg cagacgaacc ccacgtgggc aaggaacatc gcctcgatcg cggcgcccgg
     3541 cgaggcggcc gcctgcgcgg cccggtccac ccgtgccagc aggtgctcgg caacccaggc
     3601 catgaccgct tccaggatcg cgtccttgtt cgggaagtgc ttgaacagcg cgccctgggt
     3661 cacacccatg tggcgggcga tcgcagtcgt gctgatctcg ctcgggttct ggctggccgc
     3721 cagttgcacc accgcctcga ccgtgaccgc gcgccgttct tccgccggca ggtacttcgg
     3781 ttgggagttc accacgccac caaagatagt aagttattac tatcttatcg cccgtgtccc
     3841 tgcggtcaag tgcaggccgt cgccgcgggt caacgtggcc tgccaatgcg tgcagacgcg
     3901 gtgtatgttc atcctccacg ccgacggagc caccgcatgg acatccacga catcgccctg
     3961 aacctgttca ccggaatcgt cgccaggcag gacctggccg caaccagcga cgacagccgg
     4021 ctggcgctcg gccgcgaggc ctatctctgg gcggaggcgt tcatcgccgc caaggacatc
     4081 tacatccgcg agctgccggt cccggagatc gatcccggat tctgatcgac gctcctccac
     4141 tcgggttgcg gcgggctatg ctcggcgtcc ccgcacgagt gatgccgcca tgaccccacg
     4201 tttcgcctgg atcgccgccc tcctgttgct gctggccggc tgcggcagcg gcgggccggt
     4261 acggcgcatc tccgaaccgg cggccagcct gcagcaggtc agcgtgcgcg cggacgggcg
     4321 ctgggacgtg caggtgcggc tggacaactt cagcagcgtg ccgatgcgct tcagcgcggc
     4381 cacgatccag gtcgccctcg acaacggcgc cgcggccacc gtgcaggcgc agccgcagat
     4441 caccatcgga ccggaatcgg ccgatgtgtt caccgcgacg gtgacaccgc tggcggaggg
     4501 ccgcgcgcgc atcgccaccg ccctggccga tggccgcagc ctggcctaca cgctcgccgg
     4561 caccctcgag gcagggcccg aggatggcgg tccgcgcacc tacacgatca agcggaccag
     4621 cgcgctgacc ccggtgccgg ggctgccggg ggtgctgcgc tgatcggcgc cgggacacca
     4681 ttcccgccga ccatacgccg tagtatgctt gggcttccac gggccggagg gcggcccgga
     4741 ccagccactc ggaagacccc atgagcagct acaagccccc cctcgacgac atccgcttcg
     4801 ccctgttcga cgtgctcggc agcgaggcca acttcgccag gctcggcttc gccgacgcca
     4861 accgcgagtt ggtcgacgcc gtcatcgacg aagccgcgcg cttctccgaa accgtgctgg
     4921 cgccgctcaa ccgcatcggc gacgaggtcg gctgcaagtt cgacccggcg accggcgacg
     4981 tcagcgtgcc gccgggcttc aagcaggcgt tcgaccagtt cgtcgaaggc ggctggaccg
     5041 gcctgaccaa cgatccggaa ttcggcggcc agggcatgcc ggccgcgctc ggtggcgtgc
     5101 tcaccgaaat ggtggacagc gcaaacctcg cctggggcaa cttcccgatg ctttcgcacg
     5161 gcgcggtcaa ggcgctggag acctacggcg aggactggca gaaggaagtg ttcctgaagc
     5221 cgctggtggc cggcacctgg accggcacca tgtgcctgac cgagccgcac tgcggcaccg
     5281 acctcggcct gctcaagacc agggcaatcc cgaatgctga cgacggcagc tacgcgatca
     5341 ccggcaccaa gatcttcatc accgccggcg agcacgacat gtccggcaac atcgtgcacc
     5401 tggtgctggc caagctgccg gacgcaccgg ccggcgccaa gggcatctcg ctgttcgtca
     5461 ccccgaagtt caaggtcgcg cgcgacggca gcatcggcga acgcaacgcg gtgcgctgcg
     5521 gttcgatcga acacaagatg ggcatccacg gctccgccac ctgcgtgatg aatttcgatg
     5581 gcgcccaggg ctacctggtc ggcgagccgc acaagggcct gcaggcgatg ttcgtgatga
     5641 tgaactcggc gcgcctgggg gttggcgtgc agggcctggg cctgtccgaa cgcgcctacc
     5701 agaacgcgct gcgctactcg cgggaacgcc tgcagtcgcg ttcgctgtcg ggcccgaagg
     5761 cgccggacaa gccggccgac ccgatcatcg tgcaccccga cgtgcgccgc atgctgctga
     5821 cccagaaggc gctggtcgaa ggcggccgcc tgctgtcgct gcatgcctac tcgctgctgg
     5881 acgcggccga acacgaagcc gatgccggcg cgcgcgaaca cgcggatacg ctggtgggct
     5941 tcctcacccc gatctccaag gcgtgcctga ccgaatggag catcgagagc acctacaacg
     6001 cgatgcagtg cttcggcggc cacggttaca tccacgaata cggcatggag caatacgccc
     6061 gcgacgcgcg catcacgacg atttacgagg gcaccaccgg gatccaggcg ctggacctga
     6121 tcggccgcaa gaccgcggcc tcgcagggtg ccggcctgaa gctgttcctg ggcatggtcg
     6181 aagcgttcgc gaagcagcac gagggcgatc cggcgctggc cgagttcatc gccccgctgc
     6241 gggcgaaggc cgccgaatgg ggcaagctca ccatcgagac cctgcagcgc gccgcctcca
     6301 acccggacga actgggcgcg gccagccacg actacctgtt ctactcgggc tacgtggtgc
     6361 tggcctactg gtgggcacgc agcgtcgcca ccgccgacgc atcgtcgcgg ccgcaggcgt
     6421 tcaaggacgc caagcgcgag accgcgcgct tctacttcgc ccggatcctg ccgcgcacgc
     6481 tgctgcacaa ggccgcgatc gaggccggcg cggcctcgtt gatggcgatg cccgcggagc
     6541 atttcggcga gtaaccggcg gtgccagcca gcggccgcgc gccgctggct gtgtccacaa
     6601 aacgtcaccg aatgggtata agctcggtgt ccgatggaga ccgatagcgc aacgctacgt
     6661 ccggtccttg ccggaacccg aaccgcagcc gctgcggacg ggctcctgcc ctccgcgggt
     6721 acggtcaccg gattccccgg cctgcgcctg cttggccttg acgcgcatgg ccgcgccctc
     6781 gactggatga gctggcagga cgcaacctgc ctgtacgtgc gtggcgcggt ggcgtggacg
     6841 ctcggtgacc cctgcctgga gatccatggc ggcatttcac gtgccagcgg cgaacagagc
     6901 cgcgtccagc tgcatccgat catcgccgcc cgcggccacg cgcgcccgca tgcgctcgac
     6961 cccaccccgg cattgaccaa cccggcgctg ttcgcccgcg accagggcct gtgcctgtat
     7021 tgcggccgcg aattccatcg tggcgcgctg acccgcgacc acgtgcaacc ggtgtccagg
     7081 ggcggccgcg acacctggga aaacgtggtc tccgcctgct tccactgcaa ctcgcgcaag
     7141 ggcaaccgta ccccgcagca ggccggcatg ccgttgctcg cggtgccgta ccggccgagc
     7201 tggatcgagc acctgatcct gtccaaccgc aacatcctcg ccgaccagat ggcgttcctg
     7261 aaggcgcagc tgccgaagaa gacccggctg gccggttgat gcggaaacgg ctgcaggccc
     7321 gggcatgcgc tggcgtatgg acgcgtggat gtggctgcag tcgaaaggtg acgaatcacg
     7381 cgcgttgacg cggcgcgcgg ggttcgcaac actctggcct ttccccagca ggaatcggtc
     7441 atggccctga ccctcggcat caccggcatg gaccgcggca ccgaagccga ggtgcaggcg
     7501 gcgttcaagg ccgccaacgc cgagaccggc aacaaatgga cgctggtcgc cggcgacggc
     7561 gccgactacg tggtcatcga catggacagc ctgtacgggc cgatgagttg gctgcgcctg
     7621 cacgcggccg gctgcaaggt gatcggcttg acctcggtgg atcgcaacca gaccgatttc
     7681 cgcttgccgc gtccggtcag cgccaacgat ttcgcggtgc tgctgagcga gatcgccggg
     7741 gatctcgcgg ccacccccgc gcctgcggcg ccacccgccg cgtccacgcc ggcgcccgca
     7801 ccgagcgacc acctgccgcc ggcgcacgcc gtccgcgtcg accacgtcga ggatgcgccg
     7861 gcagcgtccg tggtcctggc gccggcgcct gcggaagccc cggcggcggc cgcaccggaa
     7921 ccgacccccg aagccgcgcc ggcagcgacc cgcgagttgc cacccgatcg cccgcttgca
     7981 atgtggctgg aggcgagtgg actggagcgc agggtgcgcc tgcaacgtgg cgccggcccg
     8041 gtgctgctga tcgatccacg cagcggggtc tggcacggcc cggccacgct caagccgctg
     8101 gccgcgtatt tcgaaggcac cctgctgcgc gaggatttcg ccaccccgga ggccgctgcg
     8161 tgggagagcg aagccgccgc gatcggcgcg gcgcaaccgc tggcgcgcct gcaatggctg
     8221 ggggcgttgc tcgcggggca tggcgcgctg ctgcccggca acgatccgga cggcaagttc
     8281 cgcctgacca agtggccaca gaccgaacgc gaatatccca agcacttccg gatcgccacc
     8341 gcgatgatga aggggccggc gacgatcgcc gaggtcgccg aggccagcgg cgtggcgcag
     8401 gaggaagtgg ccgacttcgt caacgccaac ctggcgaccg gctatgcgga aagcgcacgg
     8461 caggcgccgc cggaaccgga aacgccgccg cagaagccgg cgggtggcct gttcgggcgc
     8521 atgcgcgggc gctgatcgcc gccgcttaac gcaattccca cgaatccgcg ctgcggttgc
     8581 attaacgtat cggcgcgcgc atgccgcgcg cggtttcgca tggggaacag gtgatgatca
     8641 aacgactggg tgccgagttc atcggcacgt tctggctggt gctgggtggt tgcggcagcg
     8701 cggtgctggc ggcgaacttc gggggtgacg gcaacccgct cgggatcggg ctgctcgggg
     8761 tctcgctggc gttcgggctg accgtgctga ccggcgcgta tgcgctgggg cacgtctcgg
     8821 gcgggcattt caatccagcg gtgagcttcg ggctgtgggc cggcggccgc ttccaggcca
     8881 aggacctgct gccctacatc ctggcgcaga ccatcggcgc gatcttcgcc gcgtggatcc
     8941 tgttccaggt cgccagtggc cgcgccggct tcgcgatcga cgccaacgcc gccggcgcat
     9001 tcgccaccaa
//
LOCUS       DAORUK010000078         8983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_8418_length_8983_cov_18.459901, whole genome shotgun sequence.
ACCESSION   DAORUK010000078 DAORUK010000000
VERSION     DAORUK010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8418_length_8983_cov_18.459901"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..479
                     /gene="queD"
                     /locus_tag="PK818_06585"
     CDS             126..479
                     /gene="queD"
                     /locus_tag="PK818_06585"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HQY82433.1"
                     /translation="MDIFKVFTIEAAHRLPNVPAGHKCARLHGHSFRVELHVSGEPGA
                     DTGWVMDFADIKAAFKPLYEQLDHHYLNEIDGLDNPTSERLAAWIWARLKPALPLLSE
                     VVVHETCTSGCRFRG"
     gene            complement(505..2721)
                     /locus_tag="PK818_06590"
     CDS             complement(505..2721)
                     /locus_tag="PK818_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010368087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQY82434.1"
                     /translation="MGGAAGVAVSLGLAQFGVQGPWQGVALALALLALFATTTVALTA
                     GKQQDLLAEQSERGRRGEAEVSALQLEISRHTKLEQELTKAKQAAESAVMAKGEFLAT
                     MSHEIRTPLNGIVPMLDLLMHSRMQPDQQELVKTATASAQQLLRIVDDILDYSKLEAD
                     RLDLETTTFNLRELMESVITLMERTAEAKGLRLQLIIEQGVRLPVRGDPVRLRQILSN
                     LISNAIKFTERGSVTLSVKRQRETAAQHQLRFEVRDTGIGIDPEAQERLFQAFSQADA
                     STTRLYGGTGLGLAICRRIVTLMGGRIGVESELGRGSAFWFEIPLLKVQGDMPAQSGD
                     LHGGRVLLITTDQRLRMRLTMLLPNWGLRVTAVENTHDALERLRQATNQGEPWSYSVV
                     LADLASIRATALALARNLERKNQYGEARLVYLRGDESTSQDLPLATTVIPRNAEDADL
                     RSVLSASGVSWARHEAHPAPAVKAKNVDPSRKSRILLVEDNPVNLMVAQRLLQVLGAE
                     CDTAGNGQAALEKLDGGNYDLVLMDCQMPVLDGYAATRRWREIEQERKPTQRLPIVAM
                     TANAMAGDRQKCLDAGMDDYLAKPVTRGDLERCIERWRGASLSVPETLPPAEQMAARS
                     PPVLNAGVLDELREVLGGEVDKIIAVYLEDAPRLIAQLERAAVSGDPIALRVAAHTLK
                     SSSANVGATTLSDAARDLEHGARDGTLTQPASAVARIVGEYAQVRAALQAKLPAGH"
     gene            complement(2772..4808)
                     /locus_tag="PK818_06595"
     CDS             complement(2772..4808)
                     /locus_tag="PK818_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017168778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQY82435.1"
                     /translation="MTLSATDLGASELVQRLRHRLVETETQLRSLRNLGWRPDQLRAM
                     NEEIGRLHAAAERVQPAAAHAVQPLLHSLRDALAAPSMPSSNQTAQMLVLAADALAAL
                     PLATEVTDAEPLAGAAGEPKAAEAANADPFRVLIVEDDRSQALFAEAILRGAGMQPQV
                     VGMPEQMLATMERFDPDLVLMDLHMPGTSGTVLTLQIREHPRFAQVPVVFLTGDQDPE
                     RQMEVLENGGDDYILKPVRPRHLIAAVQSRVRRARALQSRIASTAQPERHPVTGLYTR
                     PALMQKLAALLPERAGGAMLVEVGNTTALRNRYGYAGFEKLMNDAGRHLGEIARTHAV
                     ARLSDNAFLVVVREQTAAQQGAYARSLRDGIGYHDFSVGDESLRLRSTVGIADLGIGF
                     ADAGAVLAAAEDAAREARAQAAGVASYVAPQVQPGGGIIDMVRLALDSEGLQLAFQPV
                     VAVAGGDQAQFQVLLRVRDAQGIERMAGEFLAAAESAGLVPKLDRWVMEQALALLQKR
                     RAESKPVRLFVSQSPRTLAQDDYANWLAQALTAASIDGTSLVIDLRLDDALVHSMLLR
                     QFCEQLVPVGVQFCLSQYRHGGDANLLLEQLPLGYLRLSADFAHQPLAQDVRDEMREV
                     IERAHRLGLQVIGQAVEDPQAAAALWMGGIDFIQGNLVQRAEHALDFDFKNATL"
     gene            complement(4917..5498)
                     /locus_tag="PK818_06600"
     CDS             complement(4917..5498)
                     /locus_tag="PK818_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HQY82436.1"
                     /translation="MDQAHLFAIGVLLAWLAGIRVYLTVFGVGLAGFFGWLDLPQALQ
                     AAQSPWVLGTSGVLAAAEFFADKIPGVDSVWDLLHTLLRVPAGAFLAAAALSPDGQLG
                     AGMLATGAGVALTSHLLKSGSRALLNTSPEPVSNWTASVTEDAAVVGGLALAFSHPWL
                     ALGMVVAVSLSFALFVWWLWRRLFRRAPRPQAG"
     gene            5596..6300
                     /locus_tag="PK818_06605"
     CDS             5596..6300
                     /locus_tag="PK818_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82437.1"
                     /translation="MRASPSHRAATGSGHPRIGFALLVLAALGFGGWGLWRAYGPQPA
                     NAADLLRQQAARIDALQQDVATLKRSDQISRQANTELQGTLAERDEEIASLRADIAFY
                     ERFVGATAQRRGLSVHELALEPQRDPQVWHFVATLTQNLNRGAVNRGRLLLSVEASDG
                     GRMHKLAWGELRQQRNAPGIEYSFKYFQQVEGDLLLPAGVKPVRVIARLVPASGAAVE
                     QGFTWSQATGGTPAGR"
     gene            6351..6722
                     /gene="erpA"
                     /locus_tag="PK818_06610"
     CDS             6351..6722
                     /gene="erpA"
                     /locus_tag="PK818_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HQY82438.1"
                     /translation="MDIVVLEQAHHAAPPAPMDFTLAAAAKVRELIAEEGNAALKLRV
                     YIQGGGCSGFQYGFEFDEGQNEDDVAIATDGAVLLVDPLSLQYLAGAIVDYSESLSGA
                     QFVIRNPNAKTTCGCGSSFTV"
     gene            6719..7645
                     /gene="nudC"
                     /locus_tag="PK818_06615"
     CDS             6719..7645
                     /gene="nudC"
                     /locus_tag="PK818_06615"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HQY82439.1"
                     /translation="MNGSGSPFAFVDGALDRADQLREDNAALQALWPRAGVIVLDEDG
                     RAFADDTRALRSARGAELGGGPGASTFLGLRDGEAWFFANASTLALHASQRVDLRQAA
                     AHWPAWQASLFAQARALQHWHQRHRHCSACGASLEFRRAGWLGVCTGCNSEHYPRTDQ
                     AVIVAVGNGGRLLLGRQASWPKRRWSLVAGFVEPGETLEQTVAREVLEETGVRVRAGS
                     ARYLASQPWPFPGSLMLGFIAEAEADEPVAGDELEQARWFSAAEVRAGIDADWTRAHE
                     DGEGIVLAPPISIARHVIRAWLAAHDATGASK"
     gene            7655..8557
                     /locus_tag="PK818_06620"
     CDS             7655..8557
                     /locus_tag="PK818_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HQY82440.1"
                     /translation="MAATLLAVIVALAVGHLAPDIATGVRQHGWFGGWLRWLNAQFPE
                     GSFWRGRHGIALALAVPLLVVGLFQVALREPLWGFVGVLFAVVVLFHCWGPRDLDLDV
                     AGVLDAPDAASRRHAASRLWAHAAGARMDGPSLVEAVFRNALRRWFGVLFWFLLLGPF
                     GAVLYRLSVLAVERDDTQLAHETAAGARAWLALLEWPVAQLMTLGLALVGNFDSVMAA
                     WREDDAFALHGDLLGTAARASVRSEIAEEAADYAESGVSASTAIIEVFGELPELRDAM
                     NLAWRILILWLAVIALFVVAGWVG"
     gene            complement(8554..>8983)
                     /locus_tag="PK818_06625"
     CDS             complement(8554..>8983)
                     /locus_tag="PK818_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HQY82441.1"
                     /translation="PVMRWIFKTAKAIPIAGAKEDPDLMQRAFDEVDAALAEGELVCI
                     FPEGALTKDGNIAPFKSGVEKILARRPVPVVPMALRNMWGSMWSRRANVKAGSALDRM
                     RVPRRLRAHVEVAVAEPMDGATATKELLEAKVRALRGDAA"
BASE COUNT         1237 a         3203 c         3173 g         1370 t
ORIGIN      
        1 gagcacagca ctcataatgc tggggtcggt ggttcgagtc cacccatagc caccactgcg
       61 atgcaagccg agaacccgcc gaacggcggg tttttcgttt ccgtaccgac atcgcgcagg
      121 gccgcatgga catcttcaag gtcttcacca tcgaagccgc gcatcgcctg ccgaacgtgc
      181 cggctgggca caagtgcgcg cgcctgcacg ggcactcgtt ccgggtcgag ctgcatgtgt
      241 ccggcgagcc gggtgcagac accggctggg tgatggattt cgccgacatc aaggccgcgt
      301 tcaagccgct gtacgagcag ctggaccacc actacctgaa cgagatcgac ggcctcgaca
      361 acccgaccag cgagcgcctt gcggcctgga tctgggcacg gctgaagccg gcgctgccgc
      421 tgctgtcgga agtcgtggtg cacgaaacct gcacatccgg ttgccgcttc cgcggctgaa
      481 ccgcaggacc ctgccgtggc gggctcagtg gcccgctggc agcttcgcct gcagggcggc
      541 gcgcacctgc gcgtattcgc cgacgatgcg cgccaccgcg cttgcgggct gggtcagggt
      601 gccgtcgcgg gcgccgtgct cgaggtcgcg cgcggcgtcc gagagggtgg tcgcgccgac
      661 gttcgcgctg gaggatttca gggtgtgcgc agccacccgc agggcgatcg ggtcgccgct
      721 caccgccgcg cgttcgagct gcgcgatcag ccgcggtgcg tcttccaggt agaccgcgat
      781 gatcttgtcg acttcgccgc cgagcacctc gcgcagctcg tcgagcacac cggcattcag
      841 caccggcggc gagcgcgcgg ccatctgttc ggccggcggc agggtctccg ggaccgacag
      901 gctggcgccg cgccagcgct cgatgcagcg ttcaaggtcg ccgcgggtca ccggcttggc
      961 caggtagtcg tccatccctg catccaggca tttctggcgg tcgccggcca tcgcgttggc
     1021 ggtcatcgcc acgatcggca ggcgttgggt gggcttgcgt tcctgctcga tctcgcgcca
     1081 gcggcgggtc gcggcatagc cgtccagcac cggcatctgg cagtccatca gcaccaggtc
     1141 gtagttgccg ccatcgagtt tttccagggc ggcctggcca ttgccggcgg tgtcgcattc
     1201 ggcgcccagc acctgcagca ggcgctgcgc gaccatcagg ttcaccgggt tgtcctcgac
     1261 cagcaggatg cggctcttgc ggcttggatc cacgttcttg gccttgacgg cgggggcagg
     1321 gtgcgcctcg tgccgcgccc acgacacccc ggacgcggac aggaccgagc gcaggtcggc
     1381 gtcttcggca ttgcgcggga tcaccgtcgt cgccagcggc aggtcctgcg aggtcgactc
     1441 gtcgccgcgc aggtagacca gccgcgcttc gccgtactgg ttcttgcgtt cgaggttgcg
     1501 tgccagcgcc agcgcggtgg cgcggatgct ggcgaggtcg gccagcacca ccgaatacga
     1561 ccagggctcg ccctggttgg tggcctggcg cagccgttcc agcgcatcgt gggtgttctc
     1621 caccgcggtc acccgcaggc cccagttcgg cagcagcatg gtcaggcgca tgcgcaggcg
     1681 ctggtcggtg gtgatcagca gcacgcggcc gccgtgcagg tcgcccgact gcgccggcat
     1741 gtcgccctgc accttgagca gcgggatctc gaaccagaac gcggaacccc gccccagttc
     1801 ggattccacc ccgatccggc cacccatcag ggtcacgatc cggcggcaga tcgccagccc
     1861 caggccggtg ccgccataca ggcgggtggt cgaggcgtcg gcctggctga aggcctggaa
     1921 caggcgctcc tgcgcctccg gatcgatgcc gatgccggta tcccgcactt cgaagcgcaa
     1981 ctggtgctgg gcggcggtct cgcgctggcg cttcacgctc agggtcacgc tgccgcgctc
     2041 ggtgaacttg atcgcgttgc tgatcaggtt gctgaggatc tggcgcaggc gcaccgggtc
     2101 gccacgcacg ggcagccgca cgccctgctc gatgatgagc tgcaggcgca ggcccttggc
     2161 ctcggcggtg cgctccatca gggtgatcac gctctccatc agctcgcgca ggttgaaggt
     2221 cgtggtctcg aggtcgaggc ggtcggcctc cagcttcgag tagtcgagga tgtcgtcgac
     2281 gatccgcaac aactgctgcg ccgaggccgt ggcggtcttc accagttcct gctggtcggg
     2341 ctgcatccgc gaatgcatca gcaggtcgag catcggcacg atgccgttga gcggggtgcg
     2401 gatctcgtgg ctcatggtgg ccaggaactc gcccttggcc atcaccgcgg attcggcggc
     2461 ctgcttggcc ttggtcagtt cctgctcgag cttggtgtgg cggctgatct ccagttgcag
     2521 cgcgctgacc tcggcttcgc cgcggcggcc acgttcgctt tgctcggcca gcaggtcctg
     2581 ctgcttgccc gcggtcagcg ccacggtggt ggtggcgaac agcgccagca acgccagggc
     2641 cagcgcgacc ccctgccacg ggccctgcac gccgaactgg gccaggccca gcgacaccgc
     2701 gacgccggcc gccccgccca gcgcaagcca gcgcgcggtc aacagggtga agccggggtc
     2761 ttggtcggtg ttcacagggt cgcgttcttg aagtcgaagt cgagggcgtg ctccgcgcgt
     2821 tgtaccagat tgccctggat gaaatcgatt ccgcccatcc acagcgcggc ggccgcctgc
     2881 gggtcctcca cggcctggcc gatcacctgc aggcccagcc ggtgcgcgcg ctcgatcacc
     2941 tcgcgcatct cgtcgcgcac atcctgcgcc agcggctggt gggcgaagtc cgccgacagc
     3001 cgcaggtagc ccagcggcag ttgctccagc agcaggttgg catcgccgcc gtggcggtac
     3061 tggctgaggc agaactgcac gccgaccggc accagttgct cgcagaactg gcgcagcagc
     3121 atcgaatgga ccagcgcatc gtccagccgc aggtcgatca ccagcgaggt gccgtcgatg
     3181 ctggccgccg tgagtgcctg cgcaagccag ttggcgtagt cgtcctgcgc cagcgtgcgc
     3241 ggcgactgcg agacgaacag gcgcaccggc ttgctctcgg cgcggcgctt ctgcagcagc
     3301 gccagcgcct gctccatcac ccagcggtcg agcttcggca ccaggccggc gctttccgcg
     3361 gcagccagga attcgccggc catgcgctcg atgccctgcg cgtcgcgcac ccgcagcagc
     3421 acctggaact gcgcctggtc gccgccggca accgcgacca ccggctggaa cgccagctgc
     3481 aggccttcgc tgtcgagcgc cagccggacc atgtcgatga tcccgccgcc gggttgcacc
     3541 tgcggtgcca cgtagctggc cacgcccgcc gcctgcgcgc gcgcctcgcg cgcggcgtcc
     3601 tcggcggcgg cgagcaccgc gccggcgtcc gcgaagccaa tgccgagatc ggcgatgccg
     3661 accgtgctgc gcaggcgcag ggattcgtcg ccgacgctga aatcgtggta gccgatgccg
     3721 tcgcgcaggg agcgcgcata cgcgccctgc tgcgcggcgg tctgctcgcg caccaccacc
     3781 aggaaggcgt tgtcgctcag gcgcgccacc gcgtgggtgc gtgcaatctc gccgaggtgg
     3841 cggccggcat cgttcatgag cttctcgaag cccgcgtagc cgtagcggtt gcgcaacgcg
     3901 gtggtgttgc cgacttccac cagcatggcg ccaccggcgc gctccgggag cagcgccgca
     3961 agtttctgca tcagcgccgg gcgcgtgtac aggccggtga ccgggtggcg ctccggctgc
     4021 gcggtgctgg cgatccggct ctgcagcgcg cgcgcgcggc ggacgcggct ctgcaccgcg
     4081 gcgatcaggt ggcgcgggcg caccggcttg aggatgtagt cgtcgccgcc gttctccagc
     4141 acttccattt gccgttccgg gtcctggtcg ccggtcagga acaccaccgg gacctgcgcg
     4201 aagcgtgggt gttcgcggat ctgcagggtc agcacggtgc cgctggtgcc cggcatgtgc
     4261 aggtccatca ggaccaggtc gggatcgaag cgctccatcg tcgccagcat ctgttccggc
     4321 attcccacca cctgcggctg catcccggcg ccgcgcagga tcgcttcggc gaacagcgcc
     4381 tggctgcggt cgtcctccac gatcagcacc cggaacggat cggcattcgc agcctcggcc
     4441 gccttcggtt cgccggccgc gccggcaagc ggttcggcgt cggtgacttc ggtagccagc
     4501 ggcaacgccg ccagcgcgtc ggcggccagc accagcatct gcgcggtctg gttggaggac
     4561 ggcatgctcg gcgcggccag cgcatcgcgc agcgaatgca gcagcggctg caccgcgtgc
     4621 gccgccgccg gctgcacgcg ttcggcggcc gcatgcaggc ggccgatttc ctcgttcatc
     4681 gcgcgcaact ggtccgggcg ccagccaagg ttgcgcaggc tgcgcaactg ggtctcggtt
     4741 tccaccaggc ggtggcgcag gcgttgcacg agttccgagg cgcccagatc tgtggcggat
     4801 agcgtcatgt caccccctgc gaacgccctt gacggagctt ccatattccc ggtgcgtgca
     4861 cagtctgacg gaaatcgcgg tggccggcgg ccagcgttgc ggtcctcacg tggccgtcac
     4921 ccggcctgcg gccgcggtgc gcgccggaac agccggcgcc agagccacca cacgaacagg
     4981 gcgaacgaca ggctgaccgc gaccaccatg cccagcgcca gccacggatg gctgaacgcg
     5041 agcgccaggc cgccgaccac ggcggcgtcc tcggtcaccg aggcggtcca gttgctgacc
     5101 ggctccggcg aggtgttcag cagcgcccgc gagcccgact tgagcaggtg gctggtcagc
     5161 gccacgccgg cgccggtggc cagcatgccg gcgccgagct ggccgtccgg cgacagtgcg
     5221 gcggcggcca ggaacgcgcc cgccggcacc cgcagcaggg tgtgcagcag gtcccagacc
     5281 gaatccacgc ccgggatctt gtcggcgaag aactccgcgg cggcgagcac gccggaggtg
     5341 cccagcaccc acggcgattg cgcggcctgc agcgcctgcg gcaggtccag ccagccgaag
     5401 aaaccggcaa ggccgacccc gaacacggtc aagtacacgc ggatgccggc cagccaggcc
     5461 agcagcacgc cgatggcgaa caggtgggct tggtccatcg tggtgctccc ggcgccatgg
     5521 ggggcgacgc cgcggcccga gtctatacgg cgttgcggct tgacgcaggc cggccggcga
     5581 tgcgataccg cggcgatgcg cgcctcccct tcgcaccgtg ccgccaccgg cagcggccat
     5641 ccgcggatcg gcttcgccct gctggtgctg gccgcgctgg ggttcggcgg ctggggcctg
     5701 tggcgcgcgt acgggccgca accggcgaat gccgccgacc tgttgcggca gcaggccgcg
     5761 cgcatcgacg cgttgcagca ggacgtcgcc accctcaagc gttccgacca gatcagccgc
     5821 caggccaata ccgagttgca gggcacgctg gccgaacgcg acgaggaaat cgccagcctg
     5881 cgcgcggaca tcgccttcta cgaacgtttc gtcggcgcca ccgcgcagcg ccgcggcctg
     5941 tccgtgcatg aactggcgct ggagccgcag cgcgacccgc aggtctggca cttcgtcgcg
     6001 accctgaccc agaacctcaa ccgcggcgcg gtcaaccgcg gccgcctgct gctgtcggtg
     6061 gaagccagcg atggcgggcg catgcacaag ctggcgtggg gcgagctgcg ccagcagcgc
     6121 aacgcgcccg ggatcgaata ttcgttcaag tacttccagc aggtcgaagg cgacctgctg
     6181 ctgccggccg gggtcaagcc ggtgcgggtg atcgcgcggc tggtcccggc ctcgggcgcg
     6241 gcggtggagc agggcttcac ctggagccag gccaccggcg gcacgcccgc gggccgctga
     6301 attccgccgg tgcggttgaa tgcgcggcca ccgcgcccca tccttggcgg atggacatcg
     6361 tcgtgctcga acaggcccac cacgccgcgc cgccggcgcc gatggatttc accctcgccg
     6421 ccgcggccaa ggtgcgcgaa ctgatcgccg aggaaggcaa cgccgcgctc aagctgcggg
     6481 tgtacatcca gggcggcggc tgctccggct tccagtacgg cttcgaattc gacgaaggcc
     6541 agaacgagga cgatgtcgcc atcgccaccg atggcgcggt cctgctggtg gatccgctca
     6601 gcctgcagta cctcgccggg gcgatcgtgg actacagcga gtcgctcagc ggcgcccagt
     6661 tcgtgatccg caacccgaac gcgaagacca cctgcgggtg cggatcgtcg ttcacggtgt
     6721 gaacgggtcc ggctcgccct tcgcgttcgt cgatggcgcg ctcgaccgcg ccgaccagtt
     6781 gcgcgaggac aacgccgccc tgcaggcact ttggccacgg gccggcgtga tcgtgctgga
     6841 tgaagacggc cgcgccttcg ccgacgacac ccgcgccctg cgcagcgcac gcggcgccga
     6901 actcggtggc ggtcccggcg ccagcacctt cctgggcctg cgcgatggcg aggcctggtt
     6961 cttcgccaac gccagcaccc tggccctgca tgcatcgcag cgggtggacc tgcgccaggc
     7021 cgccgcgcac tggccggcgt ggcaggccag cctgttcgcg caggcgcgcg cgctgcagca
     7081 ctggcaccag cgccaccgcc attgcagcgc ctgcggcgcg agcctggaat tccggcgcgc
     7141 aggctggctg ggcgtgtgca ccggctgcaa cagcgaacac tacccgcgca ccgaccaggc
     7201 ggtgatcgtc gcggtcggca acggcggccg cctgctgctg ggccgccagg ccagctggcc
     7261 gaagcggcgc tggtcgctgg tcgccggctt cgtcgaaccg ggcgaaaccc tggagcagac
     7321 cgtggcccgc gaggtgctgg aggaaaccgg cgtgcgcgtg cgcgccggca gcgcacgcta
     7381 ccttgcctcg cagccgtggc cgttcccggg ctcgctgatg ctcggcttca tcgccgaagc
     7441 ggaagcggat gagccggtag ccggcgatga actcgaacag gcgcgctggt tcagcgccgc
     7501 ggaagtgcgc gccggcatcg acgccgactg gacccgggcg cacgaggacg gcgaagggat
     7561 cgtgctggcg ccgccgatct cgatcgcgcg ccacgtgatc cgcgcgtggc tggccgcgca
     7621 cgacgccacc ggcgccagca agtagactcg acccatggcc gccaccctgc tcgccgtcat
     7681 cgtcgcgctc gccgtcggcc acctggcgcc ggacatcgcc accggcgtgc gccagcacgg
     7741 ctggttcggc ggctggttgc gctggctcaa cgcgcaattc cccgaaggca gcttctggcg
     7801 cggccgccac ggcatcgcgc tggcgctggc ggtgccgctg ctggtggtcg gcctgttcca
     7861 ggtcgcgctg cgcgagccgc tgtgggggtt cgtcggggtg ctgttcgcgg tggtggtgct
     7921 gttccattgc tggggtccgc gcgacctcga cctcgacgtt gccggggtgc tggacgcgcc
     7981 ggatgccgcg tcgcgccggc acgccgccag ccgcctgtgg gcgcacgccg cgggcgcgcg
     8041 catggacggt ccgtcgctgg tcgaggcggt gttccgcaac gccctgcggc gctggttcgg
     8101 ggtgctgttc tggttcctgc tgctgggccc gttcggcgcg gtgctgtacc gcttgagcgt
     8161 gctcgcggtg gaacgcgacg acacccagct cgcgcacgaa accgccgccg gcgcgcgcgc
     8221 gtggctggcg ctgctggaat ggccggtggc gcagctgatg accctggggc tggcgctggt
     8281 cggcaatttc gacagcgtga tggccgcctg gcgcgaggac gacgcattcg ccctgcatgg
     8341 cgacctgctc ggcaccgccg cgcgcgccag cgtgcgcagc gagatcgccg aggaagccgc
     8401 cgactacgcc gagtccgggg tatccgcgtc gaccgcgatc atcgaggtgt tcggcgaact
     8461 tccggaactg cgcgacgcga tgaacctggc gtggcggatc ctgatcctgt ggctggcggt
     8521 gatcgcgctg ttcgtggtcg ccggctgggt gggctaggcc gcatccccgc gcaacgcccg
     8581 caccttcgcc tccagcaatt ccttcgtcgc cgtcgcgcca tccatgggtt ccgcgaccgc
     8641 gacctccacg tgcgcgcgca gccggcgcgg cacgcgcatg cggtccagcg cgctgccggc
     8701 cttcacgttg gcgcggcggc tccacatgct gccccacatg ttgcgcaggg ccatcggcac
     8761 caccggcacc gggcggcgcg ccaggatctt ctccacgccg gacttgaacg gcgcgatgtt
     8821 gccgtctttc gtcaacgcgc cctccgggaa gatgcacacc agttcgccct cggccagcgc
     8881 cgcatcgact tcgtcgaacg cgcgctgcat caggtccggg tcttccttcg cgccggcgat
     8941 cgggatcgcc ttcgcggtct tgaagatcca gcgcatcacc ggg
//
LOCUS       DAORUK010000079         8842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_8625_length_8842_cov_20.284739, whole genome shotgun sequence.
ACCESSION   DAORUK010000079 DAORUK010000000
VERSION     DAORUK010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8842
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8625_length_8842_cov_20.284739"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PK818_06630"
     CDS             complement(<1..509)
                     /locus_tag="PK818_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HQY82442.1"
                     /translation="MIVLEGAPALSPFRRERLQARLNAVAPALRVVDTRHGYWIDPEQ
                     GASPDREALGRILQAEGGFAEAEAGATSRFVVPRLGTLSPWASKATELLRGAGLPVKR
                     VERGMRIDLLGWPSDAATQAALAKLLHDPMTQSLLAEREAGAALFASHPRGALESIRL
                     DDLEGANQRL"
     gene            complement(585..1418)
                     /locus_tag="PK818_06635"
     CDS             complement(585..1418)
                     /locus_tag="PK818_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HQY82443.1"
                     /translation="MKSLVFAVLSTFSLAACAQPANPQADGGQKIGVGGSTPAATKPL
                     AEPAFAEGSPEARVRDVLKQLNPKIRVDRIGVAPIPGFREVVAGGQVVYVSDDGKYLF
                     QGGLLDVAKRKDMSESALARVRADVLKTLPMADRIVYSPVGAAKHKVVVLTDVECGYC
                     RKFHNDLAEYTKRGIEVQYLAFPRAGLGSPDYRKMVSVWCADDRRKALTDAKNDRAVP
                     PRTCKTPVDMQYNAGQRMGLEGTPMLLTTDGEFLGGYLPPDVLLQRMQQVEAERAAEG
                     A"
     gene            complement(1555..2502)
                     /gene="xerD"
                     /locus_tag="PK818_06640"
     CDS             complement(1555..2502)
                     /gene="xerD"
                     /locus_tag="PK818_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HQY82444.1"
                     /translation="MSSIAERRRLARSQPPPTDADDAAIERFIDAIWAERGLSAATLD
                     AYRRDLRGLARQRGSLHGSDRQALFDYLAQRTHAGYSARSNARLLSSLRAYFGWCVRR
                     GERSDDPTALLQPPKLPRLLPKALSEAGVEALLAAPDVQTPGGLRDRAMLELMYACGL
                     RVSELVGLPATAVNLRQGVLRVSGKGGRERLVPLGEESRHWLQRWMAEARPAMSRALR
                     DPSKATALFVDGKGLPLTRQAFWALVKRCAMVAGIEPARISPHGLRHSFATHLLNHGA
                     DLRALQMLLGHGSLSTTQIYTLVAREHLKSLHARHHPRA"
     gene            2560..3000
                     /locus_tag="PK818_06645"
     CDS             2560..3000
                     /locus_tag="PK818_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HQY82445.1"
                     /translation="MQDTRGRHEPTRALIGWRLLALAYDFFPVLALWFVIAAVFTALN
                     GDSVRGGWLGLLEFVVLWLVAGAYAVPSWRRGGQTLGMRPWRLRVVGAHGGSPDPRTL
                     WMRYAVGGLSLLAGGLGFWWAWIDRDRLTWHDRISGTRMRRESK"
     gene            complement(3026..4123)
                     /gene="lptG"
                     /locus_tag="PK818_06650"
     CDS             complement(3026..4123)
                     /gene="lptG"
                     /locus_tag="PK818_06650"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HQY82446.1"
                     /translation="MMLRPKLHDHYIARMVIMTVLAAWGVLLGLDVVLAFADEFGDVG
                     KGGYTMNHAIAATCLTIPWRAYNLFPTSAVIGSLLGLGQLAATSELTALRALGLSRRR
                     LSVSVALPLALLTLLMVANGEALAPWGEERAQSLKSARNTDMVVAEYSGLWAREGDMF
                     LNARSGEQKARDGDTWLELRGVRLFQFDRDGRLQSIANVGLAEHRPGGWILRDVERTS
                     FSAKSVSRARVAEERWDSKLDEAALAANVTKPRNMGSGELRESIDYRKRNRLEAGDFE
                     EVYWGRWFYPLNVLTLCLAAIPFAFGTLRSGGYGKRLFLGIVFALGFYTLQRMATELA
                     GIYHFDYRIAFALPPAIMLAVSLWLFKRRSG"
     gene            complement(4120..5202)
                     /gene="lptF"
                     /locus_tag="PK818_06655"
     CDS             complement(4120..5202)
                     /gene="lptF"
                     /locus_tag="PK818_06655"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011052356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HQY82447.1"
                     /translation="MPKLDRYLSSEIARSVFAALVVLGMVSFTGLFADLLGEMASGKV
                     PPPLLLSQLGLRLVRYLPLILPLALLLGYLLALGRLYRDSEMHVLAAVGVGPRRLLRP
                     VMLVALPVLALIALSSLWLGPWADRVAQAMVAEANKNLLVAGLEPGRFTPMANGGVAY
                     AGTMSADGKRLGRVFVYREREQRMDVATARSGELYRDQGARMLALEEGFRVEGPSAGN
                     GLDYRLMRYARNEMQLPAADDERPGNDPAFKPTLQLLGDPSRAAVAQLHWRIAPPLLA
                     LAFVLLAVPLSRSSPRQARYGSVLLAFLAYLVGIFLMILGTQWLAEGKLPVAAGLWWL
                     LLPMLGLGVWLYLRDGRIAKAWWQSK"
     gene            5357..6838
                     /locus_tag="PK818_06660"
     CDS             5357..6838
                     /locus_tag="PK818_06660"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HQY82448.1"
                     /translation="MSLEFSLNQAPPANADVDCVVVGAFADGSLTPSATALDAASGGK
                     LKALVARGDVDGKTGKTSLLHDLPGVEAKRVLVIGLGEAAKFGVPQYIKASADAARAL
                     KTGAVASALFTISEVAVKGRDAAWNIRTAAIAADHACYRYTATLGASNKKRDEKGLER
                     FGITGDDATALARGIAIAAGVAFTRELGNLPPNICNPAYLARQAQDFAEKSDKVECEV
                     LDDAAMEALGMGSLLAVARGSANRPRLVVLKYSNGGDAKPYVLVGKGITFDTGGVNLK
                     TQGGIEEMKYDMCGAGTVLGTFVAAVGMQLPVNLVVIAAAVENAIDGNAYRPSDVITS
                     MSGKTIEVGNTDAEGRLILCDALTYAQRFEPAALVDVATLTGAMVVALGKYATGVMTK
                     KDDDLAHELIAAGETSFDRAWQMPLWDEYQGQLDSAFADVYNIGGRWAGAITAGCFLS
                     RFTEGQRWAHMDIAGVSNGDGKLGMATGRPVGLLSQWLLDRVG"
     gene            6930..7355
                     /locus_tag="PK818_06665"
     CDS             6930..7355
                     /locus_tag="PK818_06665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HQY82449.1"
                     /translation="MPRADFYLIDKPRFREQPLLLVCELARRGYAANLPILVLARDAA
                     QAEAIDDLLWAFDPDEYLPHQIAGMDEGDDDTPILVATPQMDVPSRALLINLRDAAPA
                     GSFQRVLEVVPADPAARGPLRERWKHYQALGFELNKYDM"
     gene            7366..>8842
                     /locus_tag="PK818_06670"
     CDS             7366..>8842
                     /locus_tag="PK818_06670"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HQY82450.1"
                     /translation="MTTLAPGYDPKSFESRLYAQWEASGAFAPQGDGPAYTILLPPPN
                     VTGTLHMGHAFQHTLQDALIRYHRMRGYRTLWQMGTDHAGIATEMVVSRNLAIAGQGE
                     TRDSLGRDNFIEKVWEWKQHSGDTIERQMRRLGASGDWSRSVFTMDPMASDAIVEAFV
                     RLHGQGLVYRGQRLVNWDPVLKTAISDLEVASEEENGHMWSLRYPLADGASYEHVETD
                     ADGNEVLRETRDYLVVATTRPETMLGDTAAMVHPEDPRYSGLHGKFVQLPLTGRRIPV
                     VTDDYVDRDFGTGVVKVTPAHDFNDYAVGQRHALPMINIFTDDAKVVSSRDDIPAAYR
                     GLDRFDARKLIVADLEAAGLLVEIKPHRLQVPRGDRSGQVIEPFLTDQWFVKMDTLAK
                     RGLDLVESGEVKFVPPNWINTYRHWLENIQDWTISRQLWWGHRIPAWFDDAGNIYVGR
                     NETEVRATHGLGDAPLRQDPDVLETWFSSAMFPFSTQGWPNE"
BASE COUNT         1419 a         3147 c         3010 g         1266 t
ORIGIN      
        1 aggcgctggt tcgcgccttc gaggtcgtcc aggcggatgc tctcaagcgc gccgcgcgga
       61 tgcgatgcga acagcgccgc gcccgcctcg cgctcggcca gcaacgactg cgtcatcggg
      121 tcgtgcagca gcttggccag cgcggcctgg gtggcggcat cggacggcca gccgagcagg
      181 tcgatgcgca tgccgcgctc cacccgcttc accggcaggc cggcgccgcg cagcagttcg
      241 gtggccttgc tcgcccacgg cgacagcgtg cccaggcgcg ggaccacgaa gcggctggtc
      301 gcgccggctt ccgcttcggc gaaaccgcct tcggcctgca ggatgcgccc cagcgcttcg
      361 cgatccgggc tggcgccctg ctccgggtcg atccagtagc cgtgccgggt atcgacgacc
      421 cgcaatgcgg gcgcgacggc attcaggcgg gcttgcaggc gttcgcggcg gaacggcgac
      481 agggcgggag cgccctcgag gacgatcatg tccgggacaa ccgtggcaac gggccggcta
      541 ttgtagccga ggccggcgtg gcgccggccg cggcgatgcc gggatcaggc cccctcggcg
      601 gcgcgctcgg cctcgacctg ctgcatgcgc tgcagcagca cgtccggcgg caggtagccg
      661 ccgaggaact cgccatcggt ggtcaggagc atcggggtgc cttccaggcc catccgctgg
      721 ccggcgttgt actgcatgtc gaccggcgtc ttgcaggtcc tgggcgggac cgcgcggtcg
      781 ttcttggcat cggtcagggc cttgcggcgg tcgtccgcgc accagaccga caccatcttg
      841 cggtagtccg ggctgcccag gcctgcacgc gggaacgcca ggtactggac ctcgatgccg
      901 cgcttggtgt actcggccag gtcgttgtgg aacttgcggc aatagccgca ttcgacgtcg
      961 gtgagcacca ccaccttgtg cttggcggcg ccaaccggcg agtagacgat gcggtccgcc
     1021 atcggcagcg tcttcagcac gtcggcgcgc accctggcca gcgccgattc ggacatgtcc
     1081 ttgcgcttgg cgacgtcgag caggccaccc tggaacaggt acttgccgtc gtcgctgacg
     1141 tagaccacct ggccaccggc gaccacctcg cggaagccgg ggatcggcgc caccccgatg
     1201 cggtcgaccc ggatcttcgg gttgagctgc ttgagcacgt cgcgcacgcg cgcttccggg
     1261 ctgccctcgg cgaacgccgg ttcggccagc ggtttggtcg ccgccggcgt gctgccgccg
     1321 acgccgatct tctggccgcc gtcggcctgg ggattggcag gctgcgcgca cgcagccagg
     1381 ctgaaggtgc tcaacaccgc gaagacaagg gacttcatct gggattcctg gttcaggcgg
     1441 gcatcggacc ggacgcccgc cgtggggttc catggattgt cgcatgcgcc gaccggcggc
     1501 cacccctgcc gaacggacgg catggccgtc gaatgcgcgg ccgttcagcg cgcctcaggc
     1561 ccgcggatgg tgcctggcgt gcagcgactt caggtgctcg cgggcgacca gggtgtagat
     1621 ctgggtggtc gagagcgagc cgtggccaag cagcatctgc agcgcgcgca ggtcggcacc
     1681 gtggttgagc aggtgggtgg cgaagctgtg ccggagcccg tgcgggctga tccgcgcagg
     1741 ctcgatcccg gccaccatgg cgcagcgctt gaccagggcc cagaaggcct ggcgggtcaa
     1801 gggcaggccc ttgccgtcga cgaacagggc ggtggccttc gacggatcgc gcagggcccg
     1861 ggacatcgcc ggccgcgcct cggccatcca gcgttgcagc cagtgccggg attcctcgcc
     1921 cagcggtacc agccgctcgc ggccgccctt gccgctcacc cgcagcacgc cctggcgcag
     1981 gttgaccgcg gtcgccggca gccccaccag ttcgctgacc cgcaggccgc aggcatacat
     2041 cagctccagc atcgcgcggt cgcgcagccc gccaggggtt tgcacgtcgg gcgctgccag
     2101 cagcgcctcc acccccgctt cggacaacgc cttcggcagc agtcgcggca gcttcggcgg
     2161 ctgcagcagc gcggtgggat cgtcgctgcg ctcgccgcgg cggacgcacc acccgaaata
     2221 cgcgcgcagc gacgacagca gccgcgcatt gctgcgcgcg ctgtaacccg cgtgcgtgcg
     2281 ctgcgccagg tagtcgaaca gcgcctggcg gtcgctcccg tgcagcgacc cgcgctggcg
     2341 cgccagtccc cgcaggtcgc gccggtaggc atccagggtc gccgcggaca ggccgcgttc
     2401 ggcccagatc gcgtcgatga agcgctcgat ggccgcatcg tccgcatccg tgggcggtgg
     2461 ctgcgaacgg gcgagccggc ggcgttcggc gatgctggac atgctgcaag cgtagcgtgt
     2521 cggcgcgggc gccgaatgcg gcgcctgtat ccttgcgcga tgcaggacac ccgcggacgc
     2581 cacgaaccca cgcgcgcact gatcggctgg cggctgctgg cgctggccta tgacttcttc
     2641 ccggtgctgg cgctgtggtt cgtcatcgcc gccgtgttca ccgcgctgaa cggcgactcg
     2701 gtgcgcggcg gctggctggg cctgctggag ttcgtggtgc tgtggctggt cgccggggcg
     2761 tacgcggtgc cgagctggcg gcgcggcggc cagaccctgg gcatgcggcc gtggcgcctg
     2821 cgggtggtcg gcgcgcacgg cggcagcccc gacccccgca ccctgtggat gcgctacgcg
     2881 gtcggcgggc tgtcactgct ggcgggtggg ctggggttct ggtgggcctg gatcgaccgc
     2941 gaccggctga cctggcacga ccgcatcagc ggcacgcgga tgcggcgcga gtcgaaatag
     3001 ccccgggtcc tgccggcgcg gctgctcagc cggaccggcg cttgaacagc cacagcgaca
     3061 ccgccagcat gatcgccggc ggcagcgcga aggcgatgcg gtaatcgaag tggtagatgc
     3121 cggccaactc ggtcgccatc cgctgcagcg tgtagaagcc gagggcgaac acgatcccca
     3181 ggaacaggcg cttgccgtag ccaccgctgc gcagggtgcc gaacgcgaac ggaatcgccg
     3241 ccaggcacag cgtcagcacg ttgagggggt agaaccagcg gccccagtag acctcctcga
     3301 agtcgcccgc ttccagccgg ttgcgcttgc ggtagtcgat gctctcgcgc agctcgccgc
     3361 tgcccatgtt gcgcggcttg gtcacgttcg cggccaatgc ggcctcgtcc agtttggaat
     3421 cccagcgctc ctcggccact cgggcgcggc tgaccgactt cgcgctgaag ctggtgcgtt
     3481 ccacgtcgcg caggatccag ccgccggggc ggtgctcggc caggccgacg ttggcgatgg
     3541 attgcaggcg gccgtcccgg tcgaactgga acaggcgcac gccgcgcagc tccagccagg
     3601 tatcgccatc gcgcgccttc tgctcgccgc tgcgcgcgtt caggaacatg tcgccttcgc
     3661 gcgcccacaa cccgctgtac tccgccacca ccatgtcggt gttgcgtgcg gacttcagcg
     3721 attgcgcacg ttcctcaccc cacggtgcca gagcttcgcc attggcaacc atcagcaggg
     3781 tcagcaacgc cagcggcaac gccaccgaaa cgctcaaacg tcggcgtgac aggcccagcg
     3841 cacgcaacgc ggtcagttcg gaggtggccg ccagttggcc gagtcccagc aaggaaccga
     3901 tgaccgccga ggtcgggaac aggttgtagg cgcgccatgg gatggtcagg caagtggcag
     3961 cgatggcgtg gttcatggtg tagccgccct tgccgacatc gccgaactca tcggcgaacg
     4021 ccagcaccac gtccagcccg agcagcaccc cccaggcggc gagcacggtc atgatcacca
     4081 tgcgggcgat gtaatggtcg tgcagtttgg gtcgcagcat catttcgatt gccaccaggc
     4141 tttcgcgatg cgcccgtcgc gcagatacag ccacacccca aggcccagca tcggcagcag
     4201 cagccaccac agcccggcag caaccggcag cttgccctcg gccagccatt gggtgcccag
     4261 gatcatcagg aagatgccga ccaggtaggc cagaaaggcc aacaacacgg agccgtaacg
     4321 cgcctgccgc ggcgaactgc gcgacagcgg caccgccagc agcacgaacg ccagcgccag
     4381 cagcggcggc gcgatccgcc agtgcaactg cgcaacggcc gcgcgcgagg ggtcacccag
     4441 caattgcagg gtcggcttga aggcgggatc gttgccgggg cgttcgtcgt cggccgcggg
     4501 cagctgcatc tcgttgcgcg catagcgcat cagccggtag tccaggccat tgccggcact
     4561 cggcccttcg acccggaagc cttcctccag cgccagcatg cgcgcgccct ggtcgcggta
     4621 gagctcgccg ctgcgggcgg tggccacgtc catgcgctgc tcgcgctcgc ggtacacgaa
     4681 cacccgtccc agccgcttgc cgtcggcgga catcgtcccg gcgtaggcca cgccgccatt
     4741 cgccatcggg gtgaagcggc ccggctccag ccccgccacc agcaggttct tgttggcctc
     4801 ggccaccatc gcctgcgcca cccggtcggc ccaggggccc agccacagcg aactcagtgc
     4861 gatcaacgcc agcaccggca gcgccaccag catgaccggc cgcagcagcc ggcgcgggcc
     4921 aaccccgacc gcagccagca cgtgcatctc ggaatcccgg tacagccggc ccagcgccag
     4981 caggtagccc agcaacaacg ccaacggcag gatcagcggc aggtagcgca ccagccgcag
     5041 gcccaactgc gacagcagca gcggtggcgg tacctttccg ctggccattt cgcccagcaa
     5101 gtcggcgaac agtccggtaa agctgaccat gcccagcacc accagcgccg cgaacacgga
     5161 gcgtgcgatc tcgctgctga ggtagcggtc cagcttcggc atcggccctg cttgctttag
     5221 aatccggggg agtcctgcaa ccgtgtgcag cgctgcatgc cgcgggttcc cgcggtcgcg
     5281 cattgtaagc aatgacgcaa atcatgccgc gccaacgccg cgtggacgcc ccctcctttc
     5341 cccggaactg tggtcgatga gcctcgaatt cagcctgaac caagcccctc ccgccaacgc
     5401 cgacgtcgat tgcgtggtcg tcggcgcctt tgccgacggc agcctcaccc cctccgccac
     5461 ggccctggat gccgccagcg gcggcaagct gaaggcgctg gtggcgcgcg gcgacgtcga
     5521 cggcaagacc ggcaagacgt ccctgctgca cgacctgccc ggcgtcgaag caaagcgcgt
     5581 actggtgatc gggctgggcg aagccgccaa gttcggggtg ccgcaataca tcaaggcgtc
     5641 cgccgacgcc gcgcgcgcgc tgaagaccgg cgcggtggca tcggccctgt tcacgatcag
     5701 cgaagtcgcg gtcaagggcc gcgacgccgc ctggaacatc cgcaccgccg cgatcgccgc
     5761 ggaccatgcc tgctaccgct acacggccac cctcggcgcc agcaacaaga agcgcgacga
     5821 gaagggcctg gaacgcttcg ggatcacggg cgacgacgcc accgcgctcg cccgcggcat
     5881 cgccatcgcc gccggcgtcg ccttcacccg cgagctgggc aacctgccgc cgaacatctg
     5941 caatcccgcc tacctcgccc ggcaggcgca ggacttcgcc gagaagagcg acaaggtcga
     6001 atgcgaggtg ctggacgatg ccgcgatgga agcgctcggc atgggctcgc tgctggcggt
     6061 cgcgcgcggc tccgccaacc gcccgcggct ggtggtgctg aagtactcca acggcggcga
     6121 tgcgaagccc tacgtgctgg tcggcaaggg catcaccttc gataccggcg gcgtcaacct
     6181 gaagacccag ggcggcatcg aggaaatgaa gtacgacatg tgcggcgccg gcaccgtgct
     6241 cggcaccttc gtcgccgcgg tcgggatgca attgccggtc aacctggtgg tgatcgccgc
     6301 cgcggtggag aacgccatcg acggcaacgc ctaccggccg tccgacgtga tcaccagcat
     6361 gagcggcaag accatcgagg tcggcaacac cgatgccgaa ggccgcctga tcctgtgcga
     6421 tgcattgacc tatgcgcagc gcttcgagcc ggccgcgctg gtcgacgtcg ccaccctgac
     6481 cggggcgatg gtggttgcac tcggcaagta cgccaccggg gtgatgacca agaaggacga
     6541 cgacctggcg catgagctga tcgccgccgg cgaaaccagc ttcgaccgcg cctggcagat
     6601 gccgctgtgg gacgaatacc agggccagct cgattccgcc ttcgccgacg tctacaacat
     6661 cggcggccgc tgggccggcg cgatcaccgc cggctgcttc ctgtcgcgct tcaccgaagg
     6721 ccagcgctgg gcgcacatgg acatcgccgg cgtttccaat ggcgacggca agctggggat
     6781 ggccaccggc cgcccggtcg gcctgctcag ccagtggctg ctggaccggg ttggataatg
     6841 ctctcgcagg agcgcccatg ggcgcgattg cacgtccacc atgctgcgat cgcgcccatg
     6901 gggcgctcct acaaaagcac tgacacccca tgccacgcgc cgacttctac ctgatcgaca
     6961 agccgcggtt ccgcgagcag ccgctgctgc tggtgtgcga gctggccagg cgcggctacg
     7021 cggccaacct gccgatcctg gtgctggcac gcgatgccgc gcaggcggag gccatcgacg
     7081 acctgctgtg ggcgttcgac cccgacgagt acctgccgca ccagatcgcc ggcatggacg
     7141 agggcgacga cgacaccccg atcctggtcg ccaccccgca gatggacgtg ccgtcgcgtg
     7201 cgctgctgat caacctgcgc gatgccgcgc cggccggcag cttccagcgc gtgctggagg
     7261 tggtgcccgc cgatccggcc gcgcgcggcc cgctgcgcga gcgctggaag cactaccagg
     7321 cgctcgggtt cgaattgaac aaatacgaca tgtgatgccg cgaccatgac caccctcgcc
     7381 cctggctacg accccaagtc cttcgaatcc cgcctgtatg cgcagtggga agccagcggc
     7441 gcgttcgccc cgcaaggcga cggtccggcc tacaccatcc tgctgccgcc gccgaacgtc
     7501 accggcaccc tgcacatggg ccacgcgttc cagcacaccc tgcaggacgc gctgatccgc
     7561 taccaccgca tgcgcggcta tcgcacgctg tggcagatgg gcaccgacca tgccggcatc
     7621 gccaccgaga tggtggtgag ccgcaacctc gccatcgccg gccagggcga aacccgcgac
     7681 tcactgggcc gcgacaactt catcgaaaag gtgtgggagt ggaagcagca ctccggcgat
     7741 accatcgaac ggcagatgcg ccgcctcggc gccagcggcg actggtcgcg cagcgtgttc
     7801 accatggacc cgatggcctc cgacgcgatc gtcgaggcct tcgtgcgcct gcacgggcag
     7861 ggcctggtct accgcggcca gcgcctggtc aactgggacc cggtcctgaa gaccgcgatc
     7921 tccgacctgg aagtggccag cgaggaagag aacggccaca tgtggtcgct gcgctatccg
     7981 ctggccgatg gcgccagcta cgagcacgtg gaaaccgatg ccgacggcaa cgaggtgctg
     8041 cgcgaaacgc gcgactacct ggtggtcgcc accacccgcc cggaaaccat gctcggcgac
     8101 accgcggcga tggtgcatcc ggaagatccg cgctattcgg gcctgcacgg caagttcgtg
     8161 cagctgccgc tgaccggccg ccgcatcccg gtggtcaccg acgactacgt ggaccgcgac
     8221 ttcggcaccg gcgtggtcaa ggtcaccccc gcgcacgact tcaacgacta cgcggtcggc
     8281 cagcggcacg cgctgccgat gatcaacatc ttcaccgacg atgcgaaggt ggtctcctcg
     8341 cgcgacgaca tcccggccgc gtatcgcggg ctggatcggt tcgacgctcg caagctgatc
     8401 gtcgccgacc tcgaagccgc cggcctgctg gtggagatca agccgcaccg cctgcaggtg
     8461 ccgcgcggcg accgcagcgg ccaggtgatc gagccgttcc tgaccgacca gtggttcgtg
     8521 aagatggaca cgctggcgaa gcgtggcctg gacctggtcg aatccggcga agtgaagttc
     8581 gtgccgccga actggatcaa cacctaccgc cactggctgg agaacatcca ggactggacc
     8641 atcagccgcc agctgtggtg gggccaccgc atcccggcgt ggttcgacga cgccggcaac
     8701 atctacgtgg gccgcaacga aaccgaagtg cgcgcaacgc atggcctcgg cgacgcgccg
     8761 ctgcggcagg acccggacgt gctggagacc tggttctcct cggcgatgtt cccgttcagc
     8821 acccagggct ggccgaacga ag
//
LOCUS       DAORUK010000080         8767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_8742_length_8767_cov_18.792011, whole genome shotgun sequence.
ACCESSION   DAORUK010000080 DAORUK010000000
VERSION     DAORUK010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8767
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8742_length_8767_cov_18.792011"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..635
                     /gene="upp"
                     /locus_tag="PK818_06675"
     CDS             3..635
                     /gene="upp"
                     /locus_tag="PK818_06675"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HQY82451.1"
                     /translation="MKTVEVDHPLVRHKLGKLRDIATDPVHFRQLAGEIAALLAFEAT
                     RDLPCEVLTVQTWAGPLEVQHVRGEDLTLVPILRAGLGFLPGVQALLPAAPVSVIGLR
                     RNETTHQPEGYYQRLADRMEQRTALLIDPMLATGGSAAAAIDMLKAAGCPRIKCLFLV
                     AAPEGLAALAARHPDIEVYVAAIDQRLDENAYIRPGLGDAGDRLFGTPVD"
     gene            complement(643..1035)
                     /locus_tag="PK818_06680"
     CDS             complement(643..1035)
                     /locus_tag="PK818_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HQY82452.1"
                     /translation="MPMPKVTLLFAALHVLLMLALAVPIARHRHAHKVGLGDGGDKLL
                     ARKIRVHGNFTEYVPLALLVLALLEACGLAAAAVWGFGGVLLLGRLLHAAGLSGSGGY
                     SKGRFWGTALTWLSLLLMALAGLWIGLR"
     gene            complement(1069..1959)
                     /locus_tag="PK818_06685"
     CDS             complement(1069..1959)
                     /locus_tag="PK818_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY82453.1"
                     /translation="MKLWSLSGNSQKLDGGAMFGNAPKAMWERWAKVDADNRIDLACR
                     ALLASPLNGKTVLFETGIGAFFEPALRERYGVQESRHVLLESLRAAGFEHEDIDVVVL
                     SHLHFDHAGGLLAAWEEGASPRLLFPNATFVVGAGHWRRAREPHPRDRASFIAGLPEL
                     LEASGRLQLVEGDHCPALGGSVRFHHSDGHTPGLLLAEIVGPETVDGQAHGGVVFCAD
                     LIPGRPWVHVPITMGYDRNAELLIDEKRAFLEDKLARNVHLFFTHDPGCALAQVVRDP
                     RGRFGTAHEHPELHARAFAG"
     gene            complement(1984..3678)
                     /gene="ggt"
                     /locus_tag="PK818_06690"
     CDS             complement(1984..3678)
                     /gene="ggt"
                     /locus_tag="PK818_06690"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HQY82454.1"
                     /translation="MLLAPLLAFAQVQPPASARPPGAVIASAHALATDAGIETLERGG
                     NAFDAAVAVSAVLSVVEPISSGLGGGGFFLLHDASSGRDVFVDARETAPAAATPAAYL
                     DKAGNLDRERAENGPWSAGIPGLPAALVHVSAKYGRLPLAQSLQPAIRIARDGFPVYP
                     RFARGYAERRDVMERYRGTREVFLRNGRAPVEGDVFRQPELAATLERLAAKGFDGFYR
                     GLTAKRLVAGVNAEGGRWTADELAGYRVREREPLRFGYDGWEIVTAPPPSSGGIALAE
                     MLQILEPWDLQALPQAQRVHLVVEAMRRAFRDRTFYLGDPDFVKVPARTLMDPDYAAG
                     LRATIHPGKATPSDLLSGAPTPLEDEETTHFSIIDADGNRAAVTQTVNLLFGSGLVPS
                     GTGVLLNNEMDDFALKPGTPNAFGVMGFDANAPQPGKRMLSSMTPTFMSNADKVAVIG
                     TPGGTRIITMVLLGMLGYADGLDAAQVAALPRYHHQWWPDAIGVERNALPQDVIAALR
                     AMGHTLNVPGEDNQGRRSSDAWGNMNTVLWDRRNNTLGGGSDPRTTTGKADVLPRP"
     gene            complement(3783..4043)
                     /locus_tag="PK818_06695"
     CDS             complement(3783..4043)
                     /locus_tag="PK818_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HQY82455.1"
                     /translation="MSLKINEACVNCDVCEPVCPNKAISMGPEIYVIDPALCTECVGH
                     HDEPQCVVVCPVECIDPDPLHPESIEQLLAKLAGLQAQGTTP"
     gene            complement(4102..4584)
                     /locus_tag="PK818_06700"
     CDS             complement(4102..4584)
                     /locus_tag="PK818_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82456.1"
                     /translation="MTRLLVIACLALPFAACKKEEAAKVEAVAAPMAAPTTDDVSAWR
                     AYVTDVAGRHMDGVTNSPYVYFLPGEASEGFGGLYERQLEKVEGDLGRGIIEGNMLVF
                     ASPSSTKAAELAVTGFAQVQPGTMKGVKLIFIGKPDDGEKVKAAAEPAGVKYVFVEAK
                     "
     gene            complement(4646..5143)
                     /gene="coaD"
                     /locus_tag="PK818_06705"
     CDS             complement(4646..5143)
                     /gene="coaD"
                     /locus_tag="PK818_06705"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HQY82457.1"
                     /translation="MSATRIAVYPGTFDPITNGHVDLVERASPLFETLIVGVAASPSK
                     GPALPLELRVQLAREALAGCANVEVRGFDSLLAHFVRECDAGVLLRGLRAVSDFEYEF
                     QMASMNRHLIPEVETLFLTPAEQYGFISSSLVREISRLGGDVSGFVPPAVAAALDAQW
                     RGRKG"
     gene            complement(5140..5778)
                     /gene="rsmD"
                     /locus_tag="PK818_06710"
     CDS             complement(5140..5778)
                     /gene="rsmD"
                     /locus_tag="PK818_06710"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019302879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HQY82458.1"
                     /translation="MNKVPATGRGRVPAVGGAGSVRIIGGRWRGTRLQVPHKPGLRPT
                     ADRVRETLFNWLAPVLAGARVLDLFAGSGALGLEAVSRGAASAQLVEADPKLVQALDA
                     VAARLGAAAQVRVHAGDALAWLRGCREPPFDIAFVDPPFDAGLWPAVLELLPQRLAAD
                     AWLYLECPAGHPPALSAEWALHRESATREVRYALYRRVTLAPVLHAGNQATT"
     gene            5862..7061
                     /gene="ftsY"
                     /locus_tag="PK818_06715"
     CDS             5862..7061
                     /gene="ftsY"
                     /locus_tag="PK818_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012479774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HQY82459.1"
                     /translation="MATWFRRKKPDAAPERRLSIEDLAAAFPQPAGEAAGAVDATAPG
                     PAQAAPAAGHTAEAAAPIPAEVIAAAMEDGTPATATDVGQTATPTAPPAAAGKPGWKQ
                     RIGSILTRDIAELFGRNPILDDDLLDEVETALLTADVGVTASTQLVEGLRKRMKAREF
                     ADARALLKALRTELIAMLEPVAQPLRIDAGRKPFVILTVGVNGVGKTTTIGKLARRYK
                     DEGRSLMLAAGDTFRAAAVAQLQAWGERNGVAVVAQGQDADPASVAFDALQAAKARGV
                     EVLIADTAGRLHTQQGLMAELGKIRRVLGKIDPEAPHEVLMVIDGTTGQNALSQLRQF
                     HAAVGVTGLVVTKLDGTAKGGVVFALAREFGIPIRYAGIGERPQDLRVFDAEAFVDAL
                     LPETLGR"
     gene            7058..8335
                     /locus_tag="PK818_06720"
     CDS             7058..8335
                     /locus_tag="PK818_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82460.1"
                     /translation="MKTAATEPKVPALHRLLRITGAAAVALALFALLVGWLLQPPRAV
                     GFLLDRLGDGLGLELHAGGADYRLRGTPQLVLHAFVAQRPGDADALLRAERVLVSLPW
                     RTIRARGDDLTMQRVELDAPVLDVPALQRWLATRPPGETRVPTLRDGLRIVRGRIRND
                     DWQLDGIELVVPALAAGAPLHAALGGRYLDPPLRIPFDLAIAMTRPENGAGVAAVGAL
                     TVEGDGWRMPARVHLSGPLLVGDDRVRMSPARLGVSARYHSTSSDLPFVLGAHGPLLF
                     DEAVWSLDPATLVLEGSGTIPDARARGALALGRRLVLRLGGDIASWPQAWPALPPPLS
                     ASDSPLGFALDYAGRADSGDIAALQVRRDETALDARFRLPALLDWLEADAAGSPLPPL
                     RGILRTPRVEIAGALLEGVEVEFDDPGIAPPTP"
     gene            8332..>8767
                     /locus_tag="PK818_06725"
     CDS             8332..>8767
                     /locus_tag="PK818_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HQY82461.1"
                     /translation="MNIEAEFAPRLLDWFDRNGRHDLPWQHPRTPYRVWLSEVMLQQT
                     QVAVVVPYFERFVAALPDLRALAEAPQDDVLALWSGLGYYARARNLQAAAKRCMTQHG
                     GALPRDLDALTALPGIGRSTAGAILSQAWGDPAPILDGNVKRV"
BASE COUNT         1285 a         3181 c         3069 g         1232 t
ORIGIN      
        1 gcatgaagac cgtcgaagtc gaccatccac tggtccgcca caagctcggc aagctgcgcg
       61 acatcgccac cgaccccgtg catttccgcc agctggcggg tgagatcgcc gccctgctcg
      121 cgttcgaggc cacccgcgac ctgccctgcg aggtactgac cgtgcagacc tgggccgggc
      181 cgctcgaggt gcagcacgtg cgcggcgagg acctgaccct ggtgccgatc ctgcgtgccg
      241 gcctcggttt cctgcccggc gtgcaggcct tgttgcccgc cgccccggtc agcgtgatcg
      301 ggctgcgccg caacgagacc acgcaccagc cggagggcta ttaccagcgc ctggccgacc
      361 gcatggagca gcgcaccgca ctgctgatcg acccgatgct ggccaccggc ggcagcgccg
      421 ccgccgcgat cgacatgctc aaggccgccg gctgcccccg gatcaaatgc ctgttcctgg
      481 tggccgcgcc ggaggggctg gcggcacttg cggccaggca tccggacatc gaggtgtacg
      541 tcgccgcgat cgaccagcgc ctcgacgaga acgcctacat ccgtcccggc ctgggcgatg
      601 ccggcgaccg cctgttcggc accccggtcg actgagcggc gctcagcgca acccgatcca
      661 caggccggcc agcgccatca gcagcagcga cagccaggtc agcgcggtgc cccagaaacg
      721 gcccttcgag taaccgccgc tgcccgacaa gccggccgcg tgcagcagcc ggccgagcag
      781 gagcacgcca ccgaagcccc acaccgctgc cgcggccagg ccgcacgcct ccagcaacgc
      841 cagcaccagc agcgccagcg gcacgtattc ggtgaagttg ccgtggaccc ggatcttgcg
      901 cgccagcagc ttgtcgccgc catcgccaag gccgaccttg tgcgcgtgcc ggtggcgcgc
      961 gatcggcacc gcaagcgcca gcatcagcag cacgtgcagc gcggcgaaca acagggtgac
     1021 cttgggcatc ggcatggctc gtgcgggaat gcacgcacga tgccagagtc agccggcgaa
     1081 tgcgcgcgcg tgcagttcgg ggtgttcgtg ggcggtgccg aagcggcccc tggggtcgcg
     1141 taccacctgg gccagcgcgc agcccgggtc gtgggtgaag aacaggtgca cgttgcgcgc
     1201 cagcttgtct tcgaggaagg cgcgcttctc gtcgatcagc agttcggcgt tgcggtcgta
     1261 gcccatggtg atcggcacgt gcacccacgg ccgaccgggg atcaggtcgg cgcagaacac
     1321 caccccgccg tgcgcttgcc cgtcgaccgt ctccggaccc acgatttccg ccagcaacag
     1381 ccccggggtg tggccatcgc tgtggtggaa gcgcacgctg ccgcccagcg ccgggcaatg
     1441 gtcgccttcc accagttgca gccgcccgct ggcctccagc agctccggca atcccgcgat
     1501 gaaactggcg cggtcgcgcg gatgcggttc acgcgcgcgc cgccagtggc cggcgccgac
     1561 cacgaaggtt gcgttcggga acagcaaccg cggcgatgcg ccctcctccc acgccgcgag
     1621 cagccccccg gcatggtcga agtgcaggtg cgagagcacc accacgtcga tgtcctcgtg
     1681 ctcgaagccg gccgcgcgca gcgactccag cagcacgtgg cgcgactcct gcacgccgta
     1741 gcgctcgcgc agggcgggtt cgaagaacgc gccgatcccg gtttcgaaca gcaccgtctt
     1801 gccgttcagc ggcgaggcca gcaaggcgcg gcaggcaagg tcgatgcggt tgtcggcgtc
     1861 gaccttggcc cagcgttccc acatcgcctt cggcgcgttg ccgaacatcg cgccgccgtc
     1921 gagcttctgc gaattgcctg acagggacca gagtttcatc gcgcaagtct acgccggggc
     1981 cggtcaaggc cgcggcaata cgtcggcctt gccggtcgtg gtgcgcggat cgctgccgcc
     2041 gccgagggtg ttgttgcgcc ggtcccacag caccgtgttc atgttgcccc aggcatcgct
     2101 ggaacgacgg ccctggttgt cctcgcccgg cacgttgagg gtgtggccca tcgcccgcag
     2161 cgccgcgatc acgtcctgcg gcagggcatt gcgctcgacc ccgatcgcgt ccggccacca
     2221 ctggtggtgg tagcgcggca gcgccgcgac ctgcgccgcg tccagcccgt cggcgtaacc
     2281 cagcatcccc agcaggacca tggtgatgat gcgggtgccg ccaggggtgc cgatcaccgc
     2341 caccttgtcc gcgttgctca tgaaggtcgg cgtcatcgaa ctgagcatgc gcttgccagg
     2401 ctgcggtgcg ttcgcatcaa agcccattac cccgaacgca ttgggggtgc ccggcttcag
     2461 cgcgaagtcg tccatctcgt tgttgagcag caccccggtg ccggacggca ccagcccgga
     2521 cccgaacagc aggttcaccg tctgcgtcac cgcggcgcgg ttgccgtcgg cgtcgatgat
     2581 cgagaagtgg gtggtttcct cgtcctccag cggcgtcggc gcgccggaca gcaggtcgct
     2641 gggggtggcc ttgccggggt ggatggtggc acgcaggccg gcggcgtagt cgggatccat
     2701 cagggtcctg gccgggacct tcacgaagtc gggatcgccg aggtagaagg tgcggtcgcg
     2761 gaacgcgcgg cgcatggcct cgaccaccag gtgcacgcgc tgcgcctgcg gcagggcctg
     2821 caggtcccac ggctccagga tctgcagcat ttccgccagc gcgatgccgc cggaagacgg
     2881 cggcggcgcg gtcacgatct cccagccgtc gtagccgaag cgcagcggct cgcgctccct
     2941 gacccggtag cctgccagct cgtcggcagt ccagcgcccg ccttcggcat tcaccccggc
     3001 gaccagtcgc ttcgcggtca gcccgcgata gaagccatcg aagcccttcg ccgcgagccg
     3061 ctccagggtg gccgcaagtt ccggctggcg gaacacgtca ccctcgaccg gcgcgcggcc
     3121 gttgcgcagg aacacctcgc gggtgccgcg gtagcgctcc atcacgtcgc ggcgctcggc
     3181 atagccgcgc gcgaaacgcg gatacaccgg gaagccgtcg cgcgcgatcc ggatcgccgg
     3241 ctgcagcgac tgcgccagcg gcagccgccc gtacttggcc gacacgtgca ccagcgccgc
     3301 cggcaagccg gggatgccgg ccgaccacgg cccgttctcg gcgcgttcgc ggtccaggtt
     3361 gcctgccttg tcgaggtagg cggcgggcgt ggccgcggcc ggcgcggtct cgcgcgcgtc
     3421 gacgaacacg tcgcggccgg agctggcgtc gtgcagcagg aagaagccgc cgccgccgag
     3481 gccggaactg atcggttcca ccaccgacag caccgccgac accgccaccg ccgcatcgaa
     3541 ggcattgccg ccccgctcca gggtctcgat gccggcgtcg gtggccagcg catgcgcgga
     3601 agcgatcacc gcacccggcg ggcgcgcgga cgccggcggc tgcacctgcg cgaaggcaag
     3661 cagcggcgcc agcaacaatg ccgccgcggt tgccgcgaag gcgggaatcc gggccttcgc
     3721 gccggcgtcg ggaagcaggt accgcaatcc cgcattcgcg gaagcgacag gccgggatgg
     3781 attcatggcg tggttccttg cgcctgcagc ccggccagct tcgccagcaa ctgctcgatg
     3841 gattccggat gcagcgggtc ggggtcgatg cactcgaccg ggcacaccac cacgcactgc
     3901 ggttcgtcgt ggtggccgac gcactcggtg cacagcgccg gatcgatcac gtagatctcc
     3961 ggacccatcg agatcgcctt gttcgggcaa accggttcgc agacgtcgca gttgacgcag
     4021 gcttcgttga tcttcagcga catcgtgggg gatgcgtcgc gaggcccgtc cttcgccggc
     4081 gaaggacggg cgccgtggcg tttacttcgc ttcgacgaac acgtacttga cgccggccgg
     4141 ctcggccgcg gccttcacct tctcgccgtc atcgggcttg ccgatgaaga tcaacttgac
     4201 ccccttcatg gtgccgggct gcacctgcgc gaacccggtc accgccagct cggcggcctt
     4261 ggtggaggac ggcgacgcga acaccagcat gttgccttcg atgatgccgc ggccaaggtc
     4321 gccctcgacc ttctcgagct ggcgctcgta caggccgccg aagccttcgc tggcttcgcc
     4381 cggcaggaag tacacgtacg ggctgttggt gacgccgtcc atgtggcgcc cggcgacgtc
     4441 ggtgacgtag gcccgccacg cgctcacgtc gtcggtggtg ggcgcggcca tcggcgcggc
     4501 gaccgcctcg accttcgccg cttcttcctt cttgcacgcg gcgaacggca gggccaggca
     4561 ggcgatcaca agcaatcggg tcatcgtgtt cataaggtcc ccaggttcaa cggtgatgat
     4621 gatggtacga cgccacgcgc cagcctcaac ccttgcggcc gcgccactgc gcatccagcg
     4681 cagcagccac ggcgggcggg acgaagccgg aaacatcgcc gccaaggcgc gagatctcgc
     4741 gcaccagcga cgaagaaatg aagccgtact gctcggccgg ggtcaggaac agggtctcga
     4801 cctcggggat caggtggcgg ttcatgctcg ccatctggaa ttcgtattcg aagtccgaaa
     4861 ccgcgcgcag gccgcgcagc agcacgccgg catcgcattc gcgcacgaaa tgcgccagca
     4921 gcgagtcgaa cccgcgcact tccacgttcg cgcaaccggc cagcgcctcg cgcgccagct
     4981 gcacccgcag ttccagcggc agcgccggcc ccttggacgg gctggcggcc accccgacga
     5041 tcagcgtctc gaacagcggc gatgcgcgct cgacgaggtc cacgtggccg ttggtgatcg
     5101 ggtcgaaagt gcccggatag accgcgatgc gcgtggcgct catgtcgtgg cttggttgcc
     5161 ggcgtgaagg actggcgcca gtgtaacgcg ccggtacagc gcatagcgca cctcacgggt
     5221 cgcgctttcg cgatgcaatg cccattccgc ggacagcgcc ggtgggtgcc cggccgggca
     5281 ttccaggtac agccaggcat ccgccgccag ccgctgcggc agcagttcca gcaccgccgg
     5341 ccacagcccg gcgtcgaacg gcggatcgac gaaggcgatg tcgaacggcg gctccctgca
     5401 cccacgcaac cacgccagcg catcgccggc gtggacccgc acctgcgcgg ccgcgccaag
     5461 ccgcgctgcg accgcgtcca gcgcctgcac cagtttcggg tcggcctcga ccagctgcgc
     5521 ggaggcggcc ccgcgcgaga ccgcttccag cccgagtgcg ccgctgcctg cgaacaggtc
     5581 cagtacccgc gcccccgcca gcaccggcgc cagccagttg aacagggtct cgcgaacgcg
     5641 gtcggcggtt ggccgcagcc cgggcttgtg tggcacctgc aggcgggttc cgcgccagcg
     5701 tccaccgatg atgcgcacgc tgccggcccc gccaacggca ggcactcgtc cacggccggt
     5761 ggcggggacc ttgttcatcc gggcggttgc ggggcgggtg ctaccatgcg cgccattctc
     5821 gctcattcat cggcgccccc gcggcgcgcc attcccgacg catggccacc tggttccgcc
     5881 gcaagaaacc cgacgcagca cccgagcgcc gcctcagcat cgaagacctg gcggcggcat
     5941 tcccgcagcc ggcgggtgaa gcggcggggg ccgtggatgc gaccgcaccc ggacccgcgc
     6001 aggctgcgcc cgcagccggc cacaccgccg aagccgcagc gccgatccca gccgaggtga
     6061 tcgcggcggc gatggaagac ggcacgccgg cgaccgccac cgacgtcgga cagacggcca
     6121 cgccgacggc gccaccggcg gcggccggca agcccggctg gaagcagcgc atcggcagca
     6181 tcctgacccg cgacatcgcc gagctgttcg ggcgcaaccc gatcctcgac gacgacctgc
     6241 tggacgaagt cgagaccgcg ctgctgactg ccgatgtcgg cgtcaccgcc agtacccagc
     6301 tggtcgaggg cctgcgcaag cggatgaagg cacgcgagtt cgccgatgcc cgcgcattgc
     6361 tcaaggcgtt gcgaaccgaa ctcatcgcga tgctggaacc ggtggcgcag ccgttgcgga
     6421 tcgatgccgg gcgcaagccg ttcgtgatcc tcaccgtcgg cgtcaacggc gtcggcaaga
     6481 ccaccaccat cggcaagctg gcgcgccgct acaaggacga gggccgcagc ctgatgctgg
     6541 ccgccggcga caccttccgc gccgcggcgg tcgcgcagct gcaggcctgg ggcgagcgca
     6601 acggggtggc ggtggtcgcg caggggcagg acgcggaccc ggcatcggtc gcgttcgatg
     6661 cgctgcaggc ggcgaaggcg cgcggggtcg aagtgctgat cgccgatacc gccggccgcc
     6721 tgcacaccca gcagggcctg atggcggagc tgggcaagat ccgccgggtg ctcggcaaga
     6781 tcgatcccga agcaccgcat gaagtgctga tggtgatcga cggcaccacc ggccagaacg
     6841 cgctctcgca gttgcgccag ttccatgccg cggtcggggt caccgggctg gtggtgacca
     6901 agctcgacgg caccgccaag ggcggcgtgg tgttcgcgct ggcgcgcgag ttcggcatcc
     6961 cgatccgcta cgccggcatc ggcgagcgcc cgcaggacct gcgcgtgttc gatgccgagg
     7021 ccttcgtcga cgcgctgctg cccgagaccc tcggcaggtg aagaccgcgg cgacggagcc
     7081 gaaggttccg gccctgcacc gcctgctgcg gatcaccggt gccgctgccg tcgcactggc
     7141 cctgttcgcc ctgctggtcg gctggctgct gcagccgcca cgcgcggtcg ggttcctgct
     7201 ggaccggctt ggcgacgggc tgggcctgga gctgcatgcc ggcggcgccg actaccgcct
     7261 gcgcgggacc ccgcaactgg tgctgcacgc gttcgtggcg caacgcccgg gcgacgccga
     7321 cgccctgctg cgcgccgaac gggtgctggt ctcgctgccg tggcgcacca tccgcgcgcg
     7381 cggcgacgac ctcaccatgc aacgggtgga gctggacgcg ccggtgttgg acgtgcccgc
     7441 gctgcaacgc tggctggcca cccgcccgcc aggcgagacc cgcgtcccga ccctgcgcga
     7501 cggcctgcgg atcgtgcgcg ggcgaatccg caacgacgac tggcaactcg acggaatcga
     7561 actggtcgtg cccgcgctgg ctgcgggtgc gccgctgcac gccgccctgg gtggccgcta
     7621 cctcgacccg ccgctgcgga tcccgttcga cctcgcgatc gccatgacgc ggcccgaaaa
     7681 cggtgccggg gtggcggcgg tcggcgccct caccgtcgaa ggcgatggct ggcgaatgcc
     7741 ggcacgggtg cacctgtccg gtccactgct ggtgggcgat gaccgggtgc ggatgtcgcc
     7801 ggcgcggctc ggcgtctccg cgcgctacca ctccacgtct tccgacctgc cgttcgtgct
     7861 gggcgcgcat ggcccgctgc tgttcgacga ggcggtgtgg tcgctggatc cggcgacgct
     7921 ggtgctggag gggtccggga ccatcccgga tgcacgcgcc cgcggcgcac tggcactcgg
     7981 ccgccggctg gtgctgcggt tgggtggcga catcgcctca tggccgcagg cctggccggc
     8041 gctgccgccg ccgctgtcgg catcggactc gccgcttggg ttcgcgctgg actacgccgg
     8101 ccgcgcggat tccggcgaca tcgccgcgct gcaggtgcgg cgcgacgaaa ccgcgctgga
     8161 tgcgcgcttc cggctaccgg cgctactgga ctggctggag gcggacgccg ccgggtcgcc
     8221 gttgccgccg ctgcggggga tcctgcgcac gccgcgggtg gagatcgcag gcgccctgct
     8281 ggaaggcgtg gaggtcgaat tcgacgaccc cggcatcgcc ccgcccacgc cgtgaacatc
     8341 gaggcggagt tcgccccgcg gctgctggac tggttcgacc gcaacggtcg ccacgacctg
     8401 ccctggcagc atccgcgcac gccttaccgg gtctggctca gcgaggtcat gctgcaacag
     8461 acccaggtgg cggtggtggt gccctatttc gagcggttcg tggccgccct gcccgacctg
     8521 cgcgcgctcg cggaagcacc gcaggacgat gtgctggcgt tgtggtccgg gctcggctac
     8581 tacgcccgcg cacgcaacct gcaggcggcg gcgaagcgct gcatgacgca gcacggcggc
     8641 gcgctgccgc gcgacctgga cgccctgacg gcgttgccgg ggatcggccg cagcaccgcc
     8701 ggcgcgatcc tgtcgcaggc ctggggcgat cccgcgccga tcctcgacgg caacgtcaag
     8761 cgcgtgc
//
LOCUS       DAORUK010000081         8696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_8859_length_8696_cov_18.764958, whole genome shotgun sequence.
ACCESSION   DAORUK010000081 DAORUK010000000
VERSION     DAORUK010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8859_length_8696_cov_18.764958"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PK818_06730"
     CDS             complement(<1..580)
                     /locus_tag="PK818_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HQY82462.1"
                     /translation="MKRMLINATQAEELRVAIVDGQTLYDIDIEQPSKENKKSNIYKG
                     RITRLEPSLEAAFVEYGAERHGFLPLKEISRDYFQAGVDPNKATMKELLREGQEVVVQ
                     VDKEERGNKGAALTTFISLAGRYMVLMPNSPSAGGVSRRIEGDDRAELKKAMDALAIP
                     DDMGVIIRTAGVGRDAEELQWDLDYLLSIWRAI"
     gene            1070..2038
                     /locus_tag="PK818_06735"
     CDS             1070..2038
                     /locus_tag="PK818_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012438457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQY82463.1"
                     /translation="MTEPNPPAAPASGVRTVRVLEDRDGQRIDNFLLGYLKGAPRSLV
                     YKLLRSGQVRVNGGRVRAERRLEAGDEVRIPPIRLPDPAEKGTPPQGFMDALDAAIVF
                     EDARLLALNKPSGVASHGGSGISFGAIETLRALRPKDTLELVHRLDRDTSGLLVVAKK
                     RSALGELQALLREDHGAGISKRYLALLTGRMPGGVMSVDAPLHVGLRQGGERHVQVNP
                     RGKPSLSHFRVLERRGGLSYCEVRIETGRTHQIRVHAQHIGHPIAGDDKYGDEAANKR
                     LREQVGLKRLFLHASTLEFALDGGKAPYLLNAPLAPELVEVLDRLG"
     gene            complement(2078..2419)
                     /locus_tag="PK818_06740"
     CDS             complement(2078..2419)
                     /locus_tag="PK818_06740"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HQY82464.1"
                     /translation="MSDLIPLSQAHCMPRRGSEHRVTEASIREWLPEIPGWELVENGH
                     ALRRTFTFKDYYRTMSFVNALAHVANAEDHHPDLSVHYDRCVVRFSTHDVGGLSENDF
                     ICAAKADALYA"
     gene            2588..3181
                     /locus_tag="PK818_06745"
     CDS             2588..3181
                     /locus_tag="PK818_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfuA family Fe-S biogenesis protein"
                     /protein_id="HQY82465.1"
                     /translation="MIQISESAQTHFRKLIEREAIPGLGVRLAARDAGTPRADVRLEF
                     AESGELSGDEWAVDCEGFTLWLEAASVPFLDGATIDYEAKATGGQLQIRAPKIKGVAP
                     GDAASLVERVRWTIEQEVNPQLAQHGGHVSLQEVTAEGVVVLRFGGGCHGCGMADVTL
                     KQGIEKTLVEKVPGVTAVRDATDHATGEAPYMPRDQA"
     gene            3197..4909
                     /locus_tag="PK818_06750"
     CDS             3197..4909
                     /locus_tag="PK818_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82466.1"
                     /translation="MTTAADLVAALNQRRTRFSIRPEAPGQMPPGWQHWFEAMPPVDA
                     PAPGAPAPSWVEAFVQRPLRAIPRAPKPLGRWQSLGVLLRQEWAPDEREERGLRIGVG
                     AVDMLLHIVLVGLLLWLMYLGFVALARQQPEDEAIQVEFIGRGNVAEGGGALANAGAE
                     SAAAAAAPATRATTPAPATGRPDAPLETMAVPPQPQVAASSEAPQRVREIAPPVAVEA
                     AQTLTVSEVREPQPQAFQLPPPKLRDVSQPQLQVREVQPREQVEEVATLRTQPQRSLQ
                     PRLQPADVRVPELRDQPQELEVPLPQRMATVQARNAPDRATTTAQLQVPELRGEVRDI
                     PLPPGGAPTPSAQPGRGTAAQANAGNPGAGGERGSAPAAGQAPAGTGAGAKPAAQGGR
                     GVAAAGSGAGPASTPAPGGWPGPAKSDDWGASRRNVAGTGTGSGNKGDGAGGPALFGD
                     DGAPRLPDEWTQASGIDVDRAGTWLKRPGLEYRGTRFDRYWVPQGSLLQEWVRRGIRK
                     VSIPIPGSKRRLECVVSLLQFGGGCFPVDPDVNEQPARARPPPDIPFKPALQEDNGSA
                     KPPK"
     gene            complement(5017..5457)
                     /locus_tag="PK818_06755"
     CDS             complement(5017..5457)
                     /locus_tag="PK818_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQY82467.1"
                     /translation="MTKTSMTLLALACALALSACGGGEQHPADATAGHQATSSSAGLP
                     TGNADNGRKLANEGVDGGASCVSCHGVDGNAPIIDTYPKLGGQYADYIAHSLQAYRSG
                     DRSGSATTDLMAVQAKDLSDQQIADLAAYFGGVAGQLKDLHGAN"
     gene            complement(5466..5885)
                     /locus_tag="PK818_06760"
     CDS             complement(5466..5885)
                     /locus_tag="PK818_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQY82468.1"
                     /translation="MKRLLPIAASLALALAATAVTAQDAAAPATDAAAGAEVAAPAAE
                     AAAPATPEPAAVTGDATRGKGLTYTCRGCHGVVGYKNAYPNYHVPKLGGQSPAYLNNA
                     LKEYREGKRKHPTMQAQAESFSEQDLADIAAYLSSIK"
     gene            6129..7244
                     /locus_tag="PK818_06765"
     CDS             6129..7244
                     /locus_tag="PK818_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012438222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY82469.1"
                     /translation="MSPRSGRFGPVARTGFLALVMALAACKGGKPDPGKDGADAADKG
                     PEAVPVEVATAARRSIAASYMGTAPLEARAESQVVAKTSGLALRVFVDVGQQVRAGQS
                     LVQIDRDRAALQVAQADAQVRKLEANYRRASQLVEQKMVSVNDVDQLRYDLENARASL
                     RLARLEQSYGTVTAPISGVVAARNIKPGNLVQINTPIFTIVDTSRLEATLNAPEREIE
                     TLKAGQTVQLSVDALPGKTFEGRIDRVAPVVDSGSGTFRVTCTFEGGGMLQPGMFGRI
                     RIEYDQRANALVIPRTALLEDGNAPAVYTIKAGKAARTALKLGYVDGGWVEVREGLGE
                     GDAVVIAGKAALREGSAVQVLGAKAAAAPATPRASAQ"
     gene            7241..>8696
                     /locus_tag="PK818_06770"
     CDS             7241..>8696
                     /locus_tag="PK818_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY82470.1"
                     /translation="MNPAQPGPGGAHDPLAAPAGFSLVEFATRRRVTVAMMTLTLVLF
                     GLIALGSLKVNLLPDLSYPTLTVRTDYTGAAPAEIETLISQPAEEALGVVKGLRKLKS
                     VSRTGQSDVVLEFAWGTDMDQASLDVRDKMEALQFPLEAEPPVLLRFNPSTQPIMRLA
                     LSPKDTDTGAVEEIRRLMELRRFADDDLKRKLEPVDGVAAVKVGGGLEDEVQVDIDQR
                     KLAQLGLSLESVVQRLQQENINVSGGRLEEGTQRYLVRTVNQFATVEEIGNMLLTTRA
                     AAAGSGASDQAMRVAAASGDASVMAALSQQGGGNNAVAGGMALRLKDVAVVRQGYKER
                     EAIIRLDGREAVELAIYKEGDANTVSTADALEAKLAAIRAQLPRDIELTVIEDQSQFI
                     RHAISDVKLDAVIGGVLSILIIFLFLRDGWSTFVISLSLPVSIVATFFFMGQLGLSLN
                     VMSLGGLALATGLVVDDSIVVLESIAKARERGLGI"
BASE COUNT         1316 a         2896 c         3166 g         1318 t
ORIGIN      
        1 cgatcgcgcg ccagatcgac agcaggtagt cgaggtccca ctgcagttct tcggcatcgc
       61 ggccgacgcc ggcggtgcgg atgatcacgc ccatgtcgtc cgggatggcc agcgcgtcca
      121 tcgccttctt cagctcggcg cggtcgtcac cctcgatccg gcgcgagacg ccgccggcac
      181 tgggcgagtt cggcatcagc accatgtagc ggccggccag cgagatgaac gtggtcaggg
      241 cggcgccctt gttgccgcgc tcttccttgt cgacctgcac caccacttcc tggccttcgc
      301 gcaggagctc cttcatggtc gccttgttcg ggtcgacgcc ggcctggaag tagtcgcggg
      361 agatttcctt cagcggcagg aagccatggc gctccgcgcc gtattcgacg aaggcggcct
      421 ccagcgaggg ctcgagccgg gtgatgcggc ccttgtagat gttggacttc ttgttttcct
      481 tcgacggctg ttcgatgtcg atgtcgtaca gggtctggcc gtcgacgatg gccacgcgca
      541 gttcttccgc ctgcgtggca ttgatgagca ttcgcttcat tttcgcgttc ctcgcgcgca
      601 tcgcacgcgg aaacgccgtt ggcgtttcgt ccctggatac cgcaagcgcc ccggctgcgc
      661 gttcgcgcgc cgcgtcgctc caagtgttcc agcgctacca caccacggcg aaccgcggga
      721 gcgcttgttc ccaagttcat gttgcagggc cggcgacagc cgccccctcc gttggaaatt
      781 tccgggggaa ggggcgcgtc gcacggggcg ttgttcagcg gtgtcacgga tgcccatgca
      841 tccggcgatg gtcgggtctg ccgcgcagcc gttaacatgg ccgccccggg ggcggtggca
      901 acgacactgc ggcggggatc gcggggaagt ccggctttcg gccggtgcgg tttctcagac
      961 cgtgtgatct tccagcgaaa tcaaaccctt atctcgcccg ccgagtgtaa cagatcatgc
     1021 attaccggag caggcccgtg gtgggcaggc ccgcgaggag ccgccgcgaa tgaccgaacc
     1081 gaaccctccc gccgcccccg ccagcggcgt ccgcaccgtg cgggtgttgg aggaccgcga
     1141 cggccagcgg atcgacaact tcctgctggg ctacctgaag ggagccccgc gaagcctggt
     1201 ctacaagctg ctgagatccg gccaggtgcg cgtcaacggt ggccgggtgc gggcggaacg
     1261 gcggctggag gcgggcgacg aggtgcggat cccgccgatc cggctgccgg atccggccga
     1321 gaagggcacg ccgccgcagg ggttcatgga cgcgctggac gcggcgatcg tgttcgagga
     1381 tgcccgcctg ctggcgctga acaaaccgtc cggggtcgcc agccacggcg gcagcggcat
     1441 ttcctttggt gccatcgaaa ccctgcgcgc attgcgcccg aaggacaccc tggagctggt
     1501 gcatcggctg gaccgcgaca cctcgggcct gctggtcgtc gccaagaagc gctcggccct
     1561 gggcgaactg caggcgctgc tgcgcgagga ccacggcgcc ggcatcagca agcgctacct
     1621 ggcgttgctg acggggcgga tgccgggcgg ggtgatgagc gtggacgcgc cgctgcacgt
     1681 cggcctgcgc cagggcggcg agcgccacgt gcaggtcaac cccaggggca agccctcgct
     1741 cagccacttc cgggtgctgg agaggcgcgg tggcctgagc tattgcgagg tccgcatcga
     1801 gaccggccgc acccaccaga tccgcgtgca tgcccagcac atcggccacc cgatcgccgg
     1861 cgacgacaag tacggcgatg aggcggcgaa caagcgcctg cgcgaacagg tcggcctgaa
     1921 gcggctgttc ctgcacgcct cgacgctgga attcgcgctg gatggcggca aggcgccgta
     1981 cctgctcaac gcgccgctgg cgccggaact ggtcgaagtg ctggatcggc ttggctagcg
     2041 acagggatcg ggtgatcgcg tgcgccgcga aggatgatca ggcgtagagc gcgtccgcct
     2101 tcgctgcgca gatgaaatcg ttttcgctga ggccgccgac atcgtgggtc gagaagcgaa
     2161 ccacgcagcg gtcgtagtgc acggacaggt ccggatggtg gtcttcggcg ttggcgacgt
     2221 gcgccagcgc attcacgaac gacatcgtcc ggtagtagtc cttgaacgtg aacgtcctgc
     2281 gcagggcgtg gccgttctcg accagttccc agcccgggat ctccggcaac cattcgcgga
     2341 tgctggcctc ggtaacccgg tgctcgctgc cgcgtcgcgg catgcaatgg gcctgggaca
     2401 gcggaatcag gtcggacatc ggcgtactcc tgcggtgggc gcatgaaccg cgggcatcgc
     2461 gtgcaccggg atcggggggg ggcggcgacg ctcgccgccg ccgcgtggct agaatagccg
     2521 ccaccctccg gatttccgaa tccgcgcgcc ggcagtgcgc gatcgcccga gcccccacaa
     2581 gacgaccatg atccagattt ccgaatccgc gcagacccat ttccgcaagt tgatcgagcg
     2641 cgaggccatc cccggccttg gcgtgcgcct ggccgcgcgc gatgccggca cgccacgcgc
     2701 cgacgtgcgc ctggaattcg ccgagagcgg cgagctgtcc ggcgacgagt gggcagtgga
     2761 ttgcgagggc ttcaccctgt ggctggaagc ggccagcgtg ccgttcctcg atggcgccac
     2821 catcgattac gaagcgaagg cgaccggcgg ccagttgcag atccgcgcac cgaagatcaa
     2881 gggcgtggcg ccgggcgatg ccgcctcgct ggtcgagcgc gtgcgctgga ccatcgagca
     2941 ggaggtcaat ccgcagcttg cgcagcacgg tggccacgtc agcctgcagg aagtgacggc
     3001 ggagggcgtg gtggtgttgc gtttcggcgg cggctgccat ggttgcggga tggccgacgt
     3061 gaccctcaag cagggcatcg agaagacgct ggtggaaaag gtcccgggcg tgaccgcggt
     3121 gcgcgatgcc accgaccacg cgaccggcga agcgccctac atgccgcgcg accaggcctg
     3181 acggaccccg gcgcgcgtga cgaccgccgc cgatcttgtc gccgcgctca accagcggcg
     3241 cacccgtttc agcattcgcc ccgaggcgcc cggccagatg ccgccgggct ggcagcactg
     3301 gttcgaggcg atgcccccag tcgatgcgcc ggcgcctggt gcgcccgcgc catcctgggt
     3361 ggaggcgttc gtgcagcggc cgttgcgcgc catcccgcgc gcgcccaagc cattgggacg
     3421 ctggcagtcg ctgggcgtcc tgctgcgcca ggaatgggcg ccggacgaac gcgaggagcg
     3481 cggcctgcgg atcggggtgg gggcggtcga catgctgctg cacatcgtcc tggtcggcct
     3541 gttgctgtgg ctgatgtacc tggggttcgt cgccctggcg cggcagcagc ccgaggacga
     3601 ggcgatccag gtggaattca tcggccgtgg caacgttgcc gaaggcggtg gtgcgctcgc
     3661 caacgccggt gccgagtccg ccgccgcggc tgccgcgccg gcaacccgcg ccaccacgcc
     3721 tgcgcccgcc accggtcgcc ccgatgcgcc gctggaaacc atggcggtgc cgccgcaacc
     3781 gcaggtggcg gcgagcagcg aagcgccgca acgcgtgcgc gaaatcgcac cgccggtcgc
     3841 cgtcgaagcg gcgcaaacgc tgacggtgag cgaggtgcgc gaaccgcaac cgcaagcctt
     3901 ccagttgccg ccgccgaaac tgcgcgacgt gtcgcagccg caattgcagg tgcgcgaggt
     3961 gcagccacgc gagcaggtgg aggaagtcgc caccctgcgc acgcagccgc agcggagcct
     4021 gcaaccgcgc ctgcagccgg cggacgtgcg ggtcccggaa ctgcgcgacc agccgcagga
     4081 actcgaggtg ccgctgccgc agcggatggc gaccgtgcag gcgcgcaatg cgccggaccg
     4141 ggccaccacc acggcgcagc tgcaggtgcc cgaattgcgc ggcgaagtgc gcgacatccc
     4201 gctgccgccc ggcggcgcgc ccacgccgtc ggcccaaccg ggtcgcggca ctgcggcgca
     4261 ggcgaacgcg ggcaatcccg gtgcgggtgg cgaacgcggc agcgcgcccg ccgcgggcca
     4321 ggcgccggcg ggcaccggtg ccggcgcgaa gccggccgcg cagggtggcc gcggggtggc
     4381 agcagccggc agcggtgccg gccccgccag cacgccggca ccgggtggct ggcctggccc
     4441 ggcgaagagc gatgactggg gcgcgtcgcg gcgcaatgtc gccggtaccg gcactggcag
     4501 cggcaacaag ggtgatggcg cgggcggtcc cgccctgttc ggcgatgatg gcgcgccgcg
     4561 gctgccggac gagtggaccc aggccagcgg catcgatgtc gatcgcgccg gcacctggct
     4621 gaagcgaccg ggcctggaat accgcgggac ccgcttcgac cgctactggg tgccgcaggg
     4681 gtcgctgctg caggaatggg tgcgccgcgg catccgcaag gtgtcgatcc cgatccccgg
     4741 cagcaagcgg cggttggagt gcgtggtgtc gctgttgcag ttcggcggcg gttgcttccc
     4801 ggtcgatccc gacgtcaacg agcagcccgc gcgcgcgcgg ccgccaccgg acatcccgtt
     4861 caagccggcg ttgcaggaag acaacggcag cgcgaagccg ccgaagtagg cttggctgcg
     4921 ttcttgcgcg tcattcccgc ctgcgcggga atgacgatcc gccggcaacg aaaaaggggc
     4981 ctcgcggccc cttttacaat gcgacgcccg gtgccctcag ttggcgccgt gcaggtcctt
     5041 gagctggcct gcaacgccgc cgaagtatgc ggcgaggtcg gcgatctgct ggtcgctgag
     5101 gtccttggcc tggacggcca tcaggtcggt ggtggcgctg ccgctgcggt cgcccgaacg
     5161 gtaggcctgc agcgaatggg cgatgtagtc ggcgtactgg ccgccgagct tcgggtaggt
     5221 gtcgatgatt ggtgcgttgc catcgacgcc gtggcaggac acgcagctcg cgccaccgtc
     5281 gacgccctcg ttggccagct tgcggccgtt gtccgcgtta ccggtgggca gcccggcaga
     5341 agaggacgtg gcctggtggc cggccgtggc atcggcggga tgttgttcgc cgccaccgca
     5401 ggccgacagg gcgagcgcgc aggcaagcgc gagcagggtc atggaagtct tggtcatggc
     5461 gcggctcact tgatgctcga caggtaggcg gcgatgtcgg cgaggtcctg ttcggagaag
     5521 ctctccgcct gtgcctgcat cgtcgggtgc ttgcgcttgc cttcgcggta ttccttcagc
     5581 gcgttgttga ggtacgccgg cgactgcccg ccgagcttcg gcacgtggta gttcgggtac
     5641 gcgttcttgt agccgaccac gccatggcag ccgcggcagg tgtaggtcag gcccttgccg
     5701 cgggtcgcgt cgccggtgac cgccgcgggt tcgggcgttg cgggggcggc cgcctcggca
     5761 gccggtgctg caacctcggc gccggcggcg gcatcggttg cgggagcggc ggcatcctgt
     5821 gcggtgacgg cagtggcggc gagcgcgagg gcgaggctgg cggcgatcgg caacagtcgc
     5881 ttcatggtgt gcagtatcca gggtccggtt tgcagacgcg tgccacggat gcgacacgca
     5941 agcgcgcaag tatagccatt gaatccattt cctgaccttc cccgtatgca cgccgtcatt
     6001 ggggatgatt gcgcggcttt tgccgccctg agcatgacct tcggcgcatg acgaccgccg
     6061 aagggcgggt gatatacttg actacaacac cagataggtt atttcccatg cgtgacgcaa
     6121 ccccggccat gtctccccgc agcggccgct tcggaccggt cgcccgcact ggcttccttg
     6181 ccctggtgat ggcgctggcc gcgtgcaagg gcggcaagcc cgacccgggc aaggacggcg
     6241 cggatgccgc cgacaagggc ccggaagcgg tgccggtgga ggtggccacg gctgcgcgtc
     6301 gatcgatcgc cgccagttac atgggcaccg cgccgctgga ggcgcgtgct gaatcgcagg
     6361 tggtcgccaa gacctccggc ctcgccctgc gcgtcttcgt cgatgtcggc cagcaggtcc
     6421 gcgccggcca gtcgctggtg cagatcgacc gcgaccgcgc cgcgttgcag gttgcccagg
     6481 ccgacgcgca ggtgcgcaag ctggaggcga actaccgccg cgcctcgcag ctggtcgagc
     6541 agaagatggt cagcgtcaat gatgtcgacc aactgcgcta cgacctcgag aatgcccgcg
     6601 ccagcctgcg cctggcgcgc ctggagcagt cctacggcac cgtgaccgcg ccgatctcgg
     6661 gcgtggtcgc cgcacgcaac atcaagcccg gcaacctggt gcagatcaac accccgatct
     6721 tcaccatcgt cgacacttcg cggctggaag ccaccctcaa cgcgcccgag cgcgagatcg
     6781 agaccctgaa ggccgggcag accgtgcagc tcagcgtcga tgcgctgccg ggcaagacct
     6841 tcgagggccg catcgaccgg gtggcgccgg tcgtggattc gggcagcggc accttccggg
     6901 tgacctgcac gttcgagggc ggcggcatgc tgcagccggg catgttcggc cgcatccgca
     6961 tcgaatacga ccagcgcgcg aacgcgctgg tgatcccgcg cacggcgctg ctggaggacg
     7021 gtaacgcacc cgcggtgtac accatcaagg ccggcaaggc cgcgcgcacc gcgctcaagc
     7081 ttggctatgt ggatggcggg tgggtcgagg tccgcgaagg cctgggcgag ggcgatgcgg
     7141 tggtgatcgc cggcaaggcc gcgctgcgcg agggcagtgc cgtgcaggtg ctcggcgcca
     7201 aggctgcggc cgcgccggcc acgccgcggg cgtccgcgca atgaatccgg cgcaacccgg
     7261 gccgggcggc gcgcacgatc cgctggccgc gcctgccggc ttcagcctgg tcgaattcgc
     7321 cacccgtcgc cgcgtcaccg tcgcgatgat gacgctgacc ctggtgctgt tcggcctgat
     7381 cgcgctgggc agcctcaagg tcaacctgct gcccgacctc agctatccca cgctgaccgt
     7441 gcgcaccgac tacaccggcg ccgcgccggc ggaaatcgag accctgatct cgcagcccgc
     7501 ggaagaggcg ctgggggtgg tcaagggcct gcgcaagctg aaatcggtca gccgtaccgg
     7561 gcagtcggac gttgtgctgg agttcgcgtg gggcaccgac atggaccagg ccagcctgga
     7621 cgtgcgcgac aagatggaag cgctgcagtt cccgctggaa gccgagccgc cggtgttgct
     7681 gcgcttcaat ccgtcgaccc agccgatcat gcgcctggcg ctgtcgccga aggatacgga
     7741 caccggcgcc gtggaggaaa tccgcaggct gatggaattg cgccgcttcg ccgacgacga
     7801 cctcaagcgc aagctggagc cggtcgatgg cgtggccgcg gtgaaggtgg gcggcggcct
     7861 cgaggacgaa gtgcaggtcg acatcgacca gcgcaagctg gcgcagctgg ggctgtcgct
     7921 ggaaagcgtg gtgcagcgcc tgcagcagga gaacatcaac gtctccggcg ggcgcctgga
     7981 ggagggcacc cagcgttacc tggtgcgcac cgtcaaccag ttcgccaccg tggaggagat
     8041 cggcaacatg ctgctcacca cgcgcgcggc ggcggcgggc agcggtgcct ccgaccaggc
     8101 gatgcgggtg gcggcggcca gcggcgacgc cagcgtgatg gcggcccttt cgcaacaggg
     8161 cggcggcaac aacgccgtgg ccggcggcat ggccttgcgg ctgaaggacg tggcggtggt
     8221 gcgccagggc tacaaggaac gcgaggcgat catccgcctc gacggccgcg aggcggtgga
     8281 actggcgatc tacaaggaag gcgacgccaa caccgtgtcc accgccgatg cgctggaggc
     8341 gaagcttgcg gcgatccgcg cgcaactgcc gcgcgacatc gagctgacgg tgatcgagga
     8401 ccagtcgcag ttcatccgcc atgcgatcag cgacgtcaag ctggacgcgg tgatcggcgg
     8461 cgtgctgtcg atcctgatca tcttcctgtt cctgcgcgac ggctggagca cgttcgtgat
     8521 ctcgctgtcg ttgccggtgt cgatcgtcgc caccttcttc ttcatgggcc agctcgggct
     8581 gagcctcaac gtgatgtcgc tgggcgggct ggcgctggcc accgggctgg tggtggatga
     8641 ttcgatcgtg gtgctggagt cgatcgccaa ggcgcgcgag cgcgggctcg gcatcc
//
LOCUS       DAORUK010000082         8513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_9148_length_8513_cov_20.954008, whole genome shotgun sequence.
ACCESSION   DAORUK010000082 DAORUK010000000
VERSION     DAORUK010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8513
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9148_length_8513_cov_20.954008"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PK818_06775"
     CDS             complement(<1..305)
                     /locus_tag="PK818_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQY82471.1"
                     /translation="MSQLSSACLIAGLAIAAGASAQAQAVDDPHAWLEDVSGEKSLDW
                     VKARNAKAEAEIAGSGEFKSLEAEIRAILDSDAKIPGVQKIGDHYYNFWKDKQHERGL
                     "
     gene            complement(397..663)
                     /locus_tag="PK818_06780"
     CDS             complement(397..663)
                     /locus_tag="PK818_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82472.1"
                     /translation="MTRESLDAVLRHAVILGTLAVLLIPAARGSNEWLGWLPLWLVGM
                     PLAAWWSLRGCPLPRVAVALPRRRRVQALRNRRGRASTRLARAA"
     gene            complement(725..1639)
                     /locus_tag="PK818_06785"
     CDS             complement(725..1639)
                     /locus_tag="PK818_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQY82473.1"
                     /translation="MAGTGAATITSGEPVALVLGAGGARGLAQIGVIEALQARGMNIV
                     AIAGASSGALVGGACAAGKLVELRDWMLGTSRSSMLRLLDPGFGRPALFTGDRLVRAL
                     REVVGEPRIEDLPVDFTAVAVDLVRQREVWLRRGDLWDAVRASFAIPGVFTPFLLHGR
                     ELVDGGLLAPLPITATRLSGAHRLVAVDMHGWPHAPPGEVADDCEDAGGESAPGVIGR
                     WIGRHFGGDADGDGKPDHHIGLTETMARALDTMQAQIARVQMALDPPELLIRIPRDAC
                     QFYEFWRGGELIEIGRMQAEKALDAAGY"
     gene            complement(1643..2611)
                     /locus_tag="PK818_06790"
     CDS             complement(1643..2611)
                     /locus_tag="PK818_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014648507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HQY82474.1"
                     /translation="MDRYERIIAMHRLLQAARRPVTVRRLMDELGCSRATAYRDLAYL
                     RDGLMAPLVGDGEAGFSYDKDEATRFELPGLWLNSEELHALLAAHQLLSRSSGGMLSN
                     ALAPLQQRVEKLLDAHSSGKSWPMERVRVIPHRLRKMDEHAFRVVASAVLERHRIGFD
                     YRARSTDEKTRRSVSPQRLTHYRDNWYVDAWDEERKALRSFSIDRMTAVRVLPGAAED
                     VAEEELNAHLAGSYGIFSGAPKGWATILFSAKAARWVADEHWHSQQQGRFLADGRYEL
                     KVPYSVPRELLMDVLHYGGDAEIVEPKALREQARALLQLALGNYQD"
     gene            2656..5511
                     /locus_tag="PK818_06795"
     CDS             2656..5511
                     /locus_tag="PK818_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HQY82475.1"
                     /translation="MAEPALDLSPSPGDEARTTTCYMCACRCGIKVWLQDGQVRYIQG
                     NREHPVNHGVLCAKGAAGIMQHNSPARLRKPLLRVGERGSGEFREIEWDEALDLATAW
                     LKPIRARNPDELAFFTGRDQSQALTGWWAQQFGTVNYAAHGGFCSVNMAAGGLYSVGG
                     SFWEFGEPDWEHTRLLMLWGVAEDHDSNPIKLGLGKLKARGARIIAVNPVRSGYGAIA
                     DEWIGIRPGSDGLFAFALVHELLRMDRIDLEFLVRHANAHWLVIDNPGGGDDGLFARD
                     AEGNALCWNTQANATTDANAIDIAPAVVGEYMLPDGRKARPVFQLIVDRYLDPRYSPD
                     AVSERCGIPANTIRRIARELAEAAFDSNLTLPIAWTDSWGREHAEMVGRPVAMHAMRG
                     ISAHSNGFHTCRALHLLQLLLGAVDAPGSFRYQPPFPKPVPPANRPGRTRKADGVLDA
                     PPLGFVHGPEDLVVDAQGRPRRIDHAYSWAYPLAAHGMMHTVIRNAWAGDPYRIDTLM
                     MFMANMSWNSAMNTGETMRWLTDRDEAGEYRIPRIIYSDAYASEMVAYADLVLPDTTY
                     LERFDAISLLDRPISDADGASDAIRHPVLAPETQDVEGRPRDVRGFQSVLIELGARLG
                     LPGLANDDGTPKYRDYADYIVRHERAPGVGLLAGWRGEHGESHGKGAPNPGQLQRYID
                     NGGFWREELPEHARYFKMANRGYLQWAQRFGFVPNDAPIVLQLYSETLQRFRLAAQGH
                     GAIQPPPGHRERVATYFDPLPIWYEPFEHEQASRTRFPLDAVTQRPMFMYHAWGSQNA
                     WLRQIAARNVLYLHPETGATHGIADEDWIEVCSHNGSIRVQAKFAANVQADTVWTWNA
                     IGKRKGAWRLAKDAPESGRGFLLNHLVSDLTPRGDYANADPVTGQAAWFDLRVSIRKA
                     AAGSAAVSAPQFPPLGFDQADPAPLRYGAGFRRGKRR"
     gene            5508..6254
                     /locus_tag="PK818_06800"
     CDS             5508..6254
                     /locus_tag="PK818_06800"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQY82476.1"
                     /translation="MTMLPPPSKVKLGLVIDLDTCVGCHACATSCKAWNSGGISGPLT
                     DEDPYGKHPRGVWFNRVHGYELEADAATRQPAQTLHFPRSCLHCETPACVTVCPTGAS
                     YKRAEDGIVLVDADKCIGCKLCSWACPYGAREFSETEGVMKKCTLCVDRIYNEKLDAG
                     DRQPACVQACPTRARHFGDLGDPRSDVSKLVAERGGVALMPELGYAPVNRYLPPRPRR
                     VGEHTHQQPARTDANAGSLTQRWLNRLLKR"
     gene            6284..6610
                     /locus_tag="PK818_06805"
     CDS             6284..6610
                     /locus_tag="PK818_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HQY82477.1"
                     /translation="MLVTTTPGFEGRRIVDYKGLVGGDAILGANMFRDLFAGLRDLVG
                     GRSGSYEKVLRAAKTEALEDMVAAARERGANAVVGVDLDYETIQIQDGGSMLMVSCSG
                     TAVVIE"
     gene            6613..7539
                     /locus_tag="PK818_06810"
     CDS             6613..7539
                     /locus_tag="PK818_06810"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HQY82478.1"
                     /translation="MHPAISVIAFTTLSGAGFGLWAWLGLRIAFGGPPGMYEGIGWAI
                     GLLAAAVLVAIGLASSFLHLGRPARAWRAFSQWRTSWLSREALLAVACFGAMATVFAV
                     GRSGFHPLLLRGAGGLLAVLSLATVCSTAMIYASLRPIPAWSHRLVVPAYAGFALLQG
                     ALLLPTFTSTALARGGLWWTSLALVIGLAAAKLRYWADIDRGPDMPTRGQAIGLPHRR
                     ATVFEHPHTEANYITREMAFVVARRHARLLRGVALGLFALLPLVLLGLAAARPATSIW
                     ALPLAAACSLAGALVERWLFFAEAKHMVTLYY"
     gene            complement(7576..8499)
                     /gene="hemC"
                     /locus_tag="PK818_06815"
     CDS             complement(7576..8499)
                     /gene="hemC"
                     /locus_tag="PK818_06815"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019302002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HQY82479.1"
                     /translation="MTTLRIATRKSPLALWQTEHVADRLRAAHPGLVVELVPMSTRGD
                     EVLDRSLAAIGGKGLFLKELEVAMQRGDADCAVHSLKDVPMQLEPGFTLAAILARADH
                     ADAFVSNHFDGIAALPAGARVGTSSLRRQAQLRALRPDLQLRDLRGNVNTRLAKLDAG
                     EYDAIVLACAGLQRLGFDARIRARLDAPHWLPAPAQGAIAIECRGDDAAIVALCATLD
                     DAGTRTCVGAERAMNLALHGSCHVPVAAFAQLDGERLALAGLVGCAADGRSVRAAARG
                     HGDAPEALGLAVAELLLAQGARDLLDAAAAG"
BASE COUNT         1293 a         3059 c         2921 g         1240 t
ORIGIN      
        1 aggccgcgct cgtgctgctt gtccttccag aagttgtagt agtggtcgcc gatcttctgg
       61 acgcccggga tcttggcgtc cgaatccagg atcgcgcgga tctccgcttc cagcgacttg
      121 aattcgccgg agccggcgat ctcggcctcg gccttggcgt tgcgcgcttt cacccagtcc
      181 agcgacttct cgccgctgac gtcctcgagc caggcatggg gatcgtcgac tgcctgcgcc
      241 tgcgcggagg cgccggcggc gatggcgagg ccggcgatca ggcaggcact ggacaactgg
      301 gacatggcgc gctccgggcg gaatcgtgga ccgggaacgc tagcacagcc ccgcggcagg
      361 cttgatgtgc cggaacgcct gcccgcgtcg cgccgctcag gcagcccgcg ccagccgcgt
      421 cgatgcgcgg ccgcgccggt tgcgaagcgc ctgcacgcgg cgacggcgcg gcagggcaac
      481 cgccacccgc ggcagcgggc agccgcgcag gctccaccag gccgccagcg gcatgccgac
      541 caaccatagc ggcagccagc ccagccattc gttgctgccg cgcgcggcgg gaatcagcag
      601 cacagccagg gtgccgagga tcacggcatg gcggaggacg gcatccagcg attcacgagt
      661 catggcgacg ctccgttcgg gttgcgaaca ggctgccgca ccctcttctc accggctgcg
      721 acgctcagta gccggcggcg tccagcgcct tttcggcctg catccggccg atctcgatca
      781 gctccccgcc gcgccagaat tcgtagaact ggcaggcatc gcgcgggatg cggatcagca
      841 gctccggcgg atccagcgcc atctgcacgc gcgcgatctg cgcctgcatg gtgtccagcg
      901 cacgcgccat ggtttcggtc aggccgatgt ggtggtcggg cttgccgtcg ccgtcggcgt
      961 cgccgccgaa atgcctgccg atccagcgcc cgatgacgcc cggcgcactt tcgcccccgg
     1021 cgtcttcgca atcgtcggcg acctcgcccg gcggcgcgtg tggccagccg tgcatgtcga
     1081 ccgcgaccag ccggtgcgcg ccggagagcc gggtggcggt gatcggcaac ggcgccagca
     1141 ggccgccatc gaccagctcg cggccatgca gcaggaacgg ggtgaacacc cctgggatcg
     1201 cgaacgaggc gcgcaccgca tcccacaggt cgccgcgccg cagccagacc tcgcgctggc
     1261 ggaccaggtc caccgccacc gcggtgaaat cgacgggcag gtcctcgatc cgtggctcgc
     1321 cgaccacctc gcgcagcgcg cgcaccaggc ggtcgccggt gaacagcgcc gggcggccga
     1381 agccggggtc gagcaggcgc agcatgctgc tgcggctggt gcccagcatc cagtcgcgca
     1441 actcgaccag cttgccggcc gcgcaggcgc cacccaccag cgcgccgctg gacgcaccgg
     1501 cgatcgccac gatgttcatc ccgcgcgcct gcagcgcctc gatcaccccg atctgcgcca
     1561 gtccgcgggc gccgcccgcg cccagcacca gcgcgaccgg ttccccgctg gtgatcgtcg
     1621 cagcgccggt gcccgccacc ggtcagtcct ggtagttgcc cagcgccagc tgcagcaacg
     1681 cgcgcgcctg ttcgcgcagc gccttcggct cgacgatctc ggcatcgccg ccgtagtgca
     1741 ggacgtccat cagcagttcg cgcggcaccg aatacggcac cttcagttcg tagcggccat
     1801 cggccaggaa gcggccctgc tgctgcgaat gccagtgctc gtcggccacc cagcgcgcgg
     1861 ccttggcgct gaacaggatc gtcgcccagc ccttcggcgc gccggagaag atcccgtagc
     1921 tgccggcgag gtgcgcattg agttcctctt ccgccacgtc ttcggcagcg cccggaagca
     1981 cccgcaccgc agtcatgcgg tcgatcgaga agctgcgcag cgccttgcgc tcctcgtccc
     2041 aggcatccac gtaccagttg tcgcgatagt gggtgagccg ctgcggcgac acgctgcgcc
     2101 gggtcttttc gtcggtcgag cgcgcacggt agtcgaagcc gatgcggtgg cgctccagca
     2161 ccgccgaggc gacgacgcgg aacgcgtgct cgtccatctt gcgcaggcgg tgcgggatca
     2221 cccgcacccg ctccatcggc caggacttgc cgctggagtg cgcgtccagc agcttctcga
     2281 cgcgttgctg cagcggtgcc agcgcgttcg acagcatccc gccgctgctg cgcgacagca
     2341 gctggtgcgc ggccagcagc gcgtgcaatt cctcggagtt cagccacagg cccggcaact
     2401 cgaagcgcgt ggcctcgtcc ttgtcgtagc tgaagccggc ttcgccgtcg ccgaccagcg
     2461 gcgccatcag gccatcgcgc aggtaggcga ggtcgcgata cgcggtggcg cgcgagcagc
     2521 ccaactcgtc catcagccgc ctgacggtga ccggccgccg cgcggcctgc agcaggcggt
     2581 gcatggcgat gatgcgttcg tagcgatcca tgcccggatt atgcggcagc gtgcgccgcg
     2641 cggcacaatg cgggcatggc cgaacccgcg ctggacctct cgccttcgcc gggcgacgag
     2701 gccaggacca ccacctgtta catgtgcgcc tgccgctgcg gcatcaaggt atggctgcag
     2761 gacgggcagg tccgctacat ccagggcaac cgcgagcacc cggtcaacca cggcgtgctg
     2821 tgcgcgaagg gtgcggccgg gatcatgcag cacaactcgc cggcgcggct gcgcaagccg
     2881 ctgctgcgcg tcggcgaacg cggcagcggc gaattccgcg agatcgaatg ggacgaggcg
     2941 ctggacctcg ccaccgcatg gctgaagccg atccgcgcac gcaacccgga tgaactggcg
     3001 ttcttcaccg gccgcgacca gtcgcaggcg ctgaccggct ggtgggcgca gcagttcggc
     3061 accgtcaact acgccgcgca tggcgggttc tgctcggtga acatggcggc gggcggcctg
     3121 tattcggtcg gcggcagttt ctgggaattc ggcgaaccgg actgggaaca cacccgcctg
     3181 ctgatgctgt ggggcgtggc cgaagaccac gacagcaacc cgatcaagct cgggctgggc
     3241 aagctgaagg cgcgcggcgc caggatcatc gcggtcaatc cggtgcgcag cggttatggc
     3301 gcgatcgccg acgaatggat cggcatccgt cccggcagcg acggcctgtt cgcgttcgca
     3361 ctggtccacg aattgctgcg gatggaccgc atcgatctcg aattcctggt gcgccatgcg
     3421 aatgcgcact ggctggtgat cgacaacccc ggcggcggcg atgatggcct gttcgcgcgc
     3481 gatgccgagg gcaatgcgct gtgctggaac acgcaggcga acgcaaccac ggatgcgaac
     3541 gcgatcgaca tcgcgcccgc cgtggtcggc gagtacatgc ttcccgacgg acgcaaggcg
     3601 cgcccggtgt tccagctgat cgtggaccgc tacctcgacc cacggtattc gccggacgcg
     3661 gtcagcgagc gttgcggcat tcccgcgaac accatccgcc ggatcgcacg cgaactcgcc
     3721 gaggccgcgt tcgattcgaa tctgaccttg ccgatcgcct ggaccgacag ctggggccgc
     3781 gagcacgcgg aaatggtcgg ccgcccggtg gcgatgcatg cgatgcgcgg gatcagcgcg
     3841 cacagcaacg gcttccacac ctgccgcgca ctgcacctgc tgcagctgct gctgggcgcg
     3901 gtggatgcac ccggttcgtt ccgctaccag ccgccattcc cgaaacccgt tccgccagcc
     3961 aaccgccccg gcaggacccg caaggcggat ggcgtgctgg acgcgccgcc gctgggcttc
     4021 gtgcatgggc cggaagacct ggtggtggat gcgcagggcc ggccgcgccg gatcgaccac
     4081 gcctattcgt gggcgtatcc gctggccgcg cacgggatga tgcacacggt gatccgcaac
     4141 gcctgggccg gcgatccgta caggatcgac acgctgatga tgttcatggc caacatgagc
     4201 tggaactcgg cgatgaacac cggcgagacc atgcgctggc tcaccgacag ggacgaagcc
     4261 ggcgagtacc gcatcccgcg gatcatctat tccgatgcgt atgcatcgga aatggtcgcg
     4321 tacgccgacc tggtgctgcc ggacaccacc tacctggaac gcttcgatgc gatcagcctg
     4381 ctggaccggc cgatctccga tgccgatggc gcatccgatg cgatccggca cccggtgctg
     4441 gcgccggaga cccaggatgt cgaaggcagg ccacgcgacg tgcgcggctt ccagtcggtg
     4501 ctgatcgaac ttggcgcgcg gctcgggctg ccgggcctgg ccaacgacga cggtacgccg
     4561 aagtaccgcg actacgccga ctacatcgtc cgccacgaac gcgcgcccgg cgttggcctg
     4621 ctggccggct ggcgcggtga acacggcgaa tcgcatggca agggcgcgcc gaacccgggg
     4681 caactgcagc gctacatcga caacggcggc ttctggcgcg aggagctgcc ggaacacgcg
     4741 cgctacttca agatggccaa ccgcggctac ctgcagtggg cgcaacggtt cggcttcgtg
     4801 cccaacgatg caccgatcgt gctgcagctg tattcggaga cgctgcagag attccggctg
     4861 gccgcgcaag gccatggcgc gatccagccg ccacccgggc atcgcgagcg ggtggcgacc
     4921 tacttcgacc cgctgccgat ctggtacgag ccgttcgagc acgagcaggc ttcgcgcaca
     4981 cgcttcccgc tcgatgcggt gacccagcgg ccgatgttca tgtaccacgc gtggggttcg
     5041 cagaacgcat ggttgcggca gatcgccgcc cgcaacgtcc tgtacctgca tccggaaacc
     5101 ggcgcgaccc atggcatcgc cgacgaggac tggatcgagg tctgctcgca caacggcagc
     5161 atccgcgtgc aggcgaagtt cgccgccaac gtgcaggccg acacggtgtg gacgtggaac
     5221 gcgatcggca agcgcaaggg tgcgtggagg ctggcgaagg acgcaccgga aagcgggcgg
     5281 ggcttcctgc tcaaccacct ggtctcggac ctgaccccgc gcggcgacta cgccaacgcc
     5341 gacccggtga ccgggcaggc ggcgtggttc gacctgcggg tgtcgatccg caaggcggcg
     5401 gccggtagcg cggcggtcag cgcaccgcaa ttcccgccgc tcggcttcga ccaggccgat
     5461 cccgcgccat tgcgctacgg cgccggcttc cgccgcggca agcgccgatg acgatgctgc
     5521 cgccgccgtc gaaggtgaag ctcgggctgg tcatcgacct cgatacctgc gtcggctgcc
     5581 atgcctgcgc gaccagctgc aaggcatgga attccggcgg catcagcggg ccgctgaccg
     5641 acgaggaccc ctacggcaag cacccacggg gcgtgtggtt caaccgcgtg cacggctatg
     5701 agctggaagc cgacgcggcc acccggcagc cggcccagac cctgcacttc ccgcgttcct
     5761 gcctgcactg cgaaacgccc gcctgcgtga ccgtgtgccc gaccggcgcc agctacaagc
     5821 gcgcggaaga cggcatcgtg ctggtcgacg cggacaagtg catcggctgc aagctgtgca
     5881 gctgggcctg cccgtacggc gcgcgcgagt tcagcgaaac cgaaggcgtg atgaagaaat
     5941 gcacgctgtg cgtggaccgc atctacaacg agaagctcga cgccggcgac cgccagccgg
     6001 cctgcgtgca ggcctgcccg acccgcgcgc gccatttcgg cgacctcggc gaccctcgat
     6061 ccgacgtctc gaaactggtt gccgaacgcg gtggcgtggc cttgatgccg gagctcggct
     6121 acgcacccgt caaccgctac ctgccgccgc ggccgcgccg cgttggcgaa cacacgcacc
     6181 agcaacctgc acgaaccgac gcgaacgccg gctcgctcac gcagcgctgg ttgaaccgac
     6241 tcctcaaacg ctgaaggaaa cccagatggc caaggacaac gacatcctcg tcaccaccac
     6301 ccccggcttc gagggccgcc gcatcgtcga ctacaagggc ctggtcggcg gcgacgccat
     6361 cctcggcgcc aacatgttcc gcgacctgtt cgccggcctg cgcgacctcg tcggcggccg
     6421 ctcgggcagc tacgaaaagg tgttgcgcgc ggccaagacc gaggcgctgg aagacatggt
     6481 cgccgccgcc cgcgagcgcg gcgcgaacgc ggtggtcggg gtcgacctcg actacgagac
     6541 catccagatc caggacggcg gctcgatgct gatggtgtcg tgttcgggga ccgcggtggt
     6601 gatcgagtaa cgatgcaccc cgccatctcg gtcatcgcct tcaccaccct gtccggcgcc
     6661 ggtttcggcc tgtgggcctg gctgggcctg cggatcgcgt tcggcggccc gcccgggatg
     6721 tacgagggca tcggctgggc catcggcctg ctggccgccg ccgtgctggt cgccatcggc
     6781 cttgccagtt cgttcctgca cctcggcagg cccgcgcgcg cctggcgtgc gttctcgcaa
     6841 tggcgcactt cgtggttgtc gcgcgaggcc ttgctcgcgg ttgcctgctt cggagcgatg
     6901 gcaaccgtgt tcgcggtcgg ccgcagcggt ttccatccgc tgctgctgcg gggcgccggc
     6961 ggcttgctgg cggtgctctc gctcgccacc gtctgcagca ccgcgatgat ctacgcgtcg
     7021 ctgcggccga tccccgcatg gtcgcatcgc ctggtggtgc cggcctacgc cggcttcgcg
     7081 ctgttgcagg gcgcgctgtt gctgcccacg ttcacctcga ccgcgctcgc gcgcggcggg
     7141 ctgtggtgga ccagcctggc gctggtgatc ggcctggccg cggccaagct gcgctattgg
     7201 gcggacatcg accgcgggcc cgacatgccc acccgcgggc aggcgatcgg cctgccgcac
     7261 cggcgtgcca cggtgttcga acacccgcac accgaggcca actacatcac ccgcgagatg
     7321 gccttcgtgg tggcgcgcag gcatgcccgc ctgctccgcg gcgtcgccct cggcctgttc
     7381 gcactgctgc cgctggtgct gctggggctg gcagcggcgc ggccggccac gtcgatctgg
     7441 gcattgccgc ttgccgccgc gtgcagcctg gcgggcgcgc tggtggaacg ctggctgttc
     7501 ttcgccgagg cgaagcacat ggtcaccctg tactactgaa accggcggcg cgcgccgcgg
     7561 tccggcgcaa ggcggctagc ccgccgccgc tgcgtcgagc aggtcccgcg caccctgcgc
     7621 cagcaacaac tccgcaaccg ccagccccag cgcttccggc gcatcgccgt ggccccgtgc
     7681 cgccgcgcgc acgctgcggc catcggcggc gcaaccgacc agcccggcca gcgcgagccg
     7741 ctccccgtcg agttgcgcga acgcggccac cggcacgtgg caactgccat gcagggccag
     7801 gttcatcgcc cgctcggcac cgacgcaggt gcgggtgccg gcatcgtcca gcgtcgcgca
     7861 caaggcgacg atcgccgcgt cgtcgccgcg gcattcgatc gcgatcgcgc cctgtgccgg
     7921 cgccggcaac cagtgcggcg catccagccg cgcacggatc cgcgcatcga acccgagccg
     7981 ctgcaagccg gcgcaggcca gcacgatggc gtcgtactcg ccggcatcca gcttcgccag
     8041 gcgggtgttg acgttgccgc gcaggtcgcg cagctgcagg tccgggcgca acgcgcgcaa
     8101 ctgcgcctgc cggcgcagcg aagaggtgcc gacgcgcgcg cccgcgggca acgccgcgat
     8161 gccgtcgaag tggttgctga cgaaggcatc ggcatggtct gcgcgggcca ggatcgcggc
     8221 cagggtgaag cccggctcca gctgcatcgg cacgtccttc agcgaatgca ccgcgcagtc
     8281 ggcgtcgccg cgctgcatcg cgacctccag ttccttgagg aacaggccct tgccgccgat
     8341 cgccgccagc gagcggtcca gcacctcgtc gccgcgggta ctcatcggca ccagctcgac
     8401 caccaggccg ggatgcgcgg cgcgcaggcg gtcggcgaca tgctcggtct gccagagggc
     8461 gagcgggctc ttgcgggtgg cgatgcgcag ggtggtcatg cgcgcattat cgc
//
LOCUS       DAORUK010000083         8505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_9163_length_8505_cov_17.648047, whole genome shotgun sequence.
ACCESSION   DAORUK010000083 DAORUK010000000
VERSION     DAORUK010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8505
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9163_length_8505_cov_17.648047"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /gene="dksA"
                     /locus_tag="PK818_06820"
     CDS             <1..621
                     /gene="dksA"
                     /locus_tag="PK818_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012438163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HQY82480.1"
                     /translation="AKPAPAKATPAAKVAKPAVAPARPVVVPPPPHPRKLGKVAVAVS
                     VAARTSAPVPKGKYKVVPYQTDDASGRPIIPPGYRPSADEEYMSPLQLEYFRQMLLVR
                     RAELVDESKQTIENLKEEVRDVGDEAERATRETENSLELRTRDRYRKLIGKIDSTLKR
                     VDGGDYGYCVDTGEEIGLDRLEARPQAERTIDAQERWEHLQKQMGD"
     gene            complement(671..1900)
                     /locus_tag="PK818_06825"
     CDS             complement(671..1900)
                     /locus_tag="PK818_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY82481.1"
                     /translation="MTEAAASPLQAAGFRWLLLYRILTLLSYQVVAVTVGWHVYEITG
                     DPWSLGLIGLAEVIPFFCVAPFSGYLVDHLPRRKLGAAACTVLAVNAGVLALVASGGL
                     ATDGTWPIYAAIAVGGVGRSFLGPVYNALFARALKREQFARGAGMGSVVFQAGLVLGP
                     AIGGLLVGAAGKSVAYALAAGFAIAAALALLRMPVTEPAPQLQRGPVLASIGEGLRFV
                     FGHQVLLAALALDMFAVLFGGAISLAPAFIKDVLHAGPEALGILRGAPALGSVAVAVW
                     LSRHPLQANAGRILLAAVVGFGACMVGFGLSQVFWLSALFLLLSGVFDGVSVVLRTTI
                     LQLATPDAMRGRVSSVNSIFISSSNELGAFYAGSMARLLGLVPGVVLGGFVTMGVAAF
                     TAAKAPKLRRLDLRELQ"
     gene            complement(1897..2340)
                     /locus_tag="PK818_06830"
     CDS             complement(1897..2340)
                     /locus_tag="PK818_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HQY82482.1"
                     /translation="MSGTVFPLEPTAADAQAAIAEEFAFFGDWSERYQYLIDLGRKLP
                     HLPDDCKVEQHRLHGCQSLVWVVASGDASRLDFTAASDSAIVSGLVFLALRVYSGRSA
                     AEILATEPDYIAAIGLARHLSPTRSNGLAALLAFIREHARTAPAA"
     gene            complement(2337..3728)
                     /gene="cysS"
                     /locus_tag="PK818_06835"
     CDS             complement(2337..3728)
                     /gene="cysS"
                     /locus_tag="PK818_06835"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQY82483.1"
                     /translation="MNPSSSRLHLHNTLTRRLEPFAPLDAACPTMYVCGPTVYNYVHI
                     GNARGPVVFGVLAALLRRRFGGLRYARNITDVDDKINAAARDLGVPIATITDQYAAAY
                     RDDMAALGVSGEFAPDIEPAATAHMPQMIAMIERLIADGHAYAAEGHVLFAVSSFDGY
                     GKLSRRDTEEMLAGARVEVAPYKRDAGDFVLWKPSGDDLPGWDSPWGRGRPGWHIECS
                     AMAEAHLGETIDIHAGGVDLQFPHHENEVAQSECAHGGAPFARFWLHNGMLNFGGAKM
                     AKSVGNIQRVHDLVRQHPPEALRYALLSAHYRQPLEWSDGLIEQSVRTLDRLYGTLRD
                     LAEVEADVAIPGSIEAALDDDLNTPQALAEIARIAGEARRADSAAEKARLKTELLGAG
                     RVLGLLQQAPADWFARGAGNDDDARIQGLIDERIEAKKSRDFARADAIRQQLADEGIL
                     LEDTPQGVRWKRA"
     gene            3816..4277
                     /locus_tag="PK818_06840"
     CDS             3816..4277
                     /locus_tag="PK818_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005996827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82484.1"
                     /translation="MLRLALCCLFVPVAGLLVAPRVHAQEVVRETVVPQENVRFDYAQ
                     VLAVSPVYQTLRATRMERVCDARQPSGNGLSKVVSAVKDRLDGATSKEEREAGLSNCR
                     MAPVVREYRRPIAYDVDYAYKGSKFRTRLAQDPGNRLRVRVSITPQPMAGP"
     gene            4532..5536
                     /locus_tag="PK818_06845"
     CDS             4532..5536
                     /locus_tag="PK818_06845"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002802340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HQY82485.1"
                     /translation="MKHFLNTQDWQRAELDALLADAARFKREPLGDALKGRSIALVFF
                     NPSMRTRTSFELGAFQLGGHAIVLQPGKDAWPIEFDLGTVMDGDTEEHIAEVARVLGR
                     YVDLIGVRAFPKFVDWQRDREDIVLKSFAKYSPVPVINMETITHPCQELAHALALQER
                     FGTSDLRGKKYVLTWTYHPKPLNTAVANSALTIATRMGMDVTLLCPTPDYVLDQRYMG
                     WAAQNVAESGGSLQVSHDIESAYAGADVVYAKSWGALPFFGNWEPEKPIRDRYKHFIV
                     DEAKMALTNDGVFSHCLPLRRNVKATDAVMDSPRCVAIDEAENRLHVQKAIMAALIQQ
                     "
     gene            5591..6808
                     /locus_tag="PK818_06850"
     CDS             5591..6808
                     /locus_tag="PK818_06850"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HQY82486.1"
                     /translation="MTASDSKDIVLAFSGGLDTSFCVPYLQEQGWNVHTVFADTGGVD
                     AEERATIEARAEELGVASHVTVDGGPAIWSGFVKPFVWAGEGYQGQYPLLVSDRYLIV
                     DASLARARALGTNAIAHGCTGMGNDQVRFDLAVKAQGDYRIVAPIREIQKQHTQTRAF
                     EQAYLEERGFGVRAKQKAYTINENLLGLTMSGGEIDAWEAPGDGARGWCARREQWPAD
                     ALEVTLRFDNGEAVALDGQALAGEKILAKLNTMFAQYGVGRGMYTGDTTIGLKGRIVF
                     EAPGLAALLAAHRALEEAVLTKQQNRCKPEVARKWVELVYEGFYHDPLKTDLEAFLAS
                     SQRMVNGEVVLRTSGGRVDAVAVRSPHILNSKGATYAQSADWGVEEAEGFIKLFGMSS
                     TLWAQVNGGQPDA"
     gene            6801..7898
                     /locus_tag="PK818_06855"
     CDS             6801..7898
                     /locus_tag="PK818_06855"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HQY82487.1"
                     /translation="MPELLPDVLRHLENLVAFDTRNPPRLIGTGGIFDYLRAQLPGFR
                     CEVVDHGDGAVSLLAVRGNPRRVFNVHLDTVPSSEAWSADPHVLRVTHDRAIGLGACD
                     IKGAAAALVAAANAMNGDAAFLFSSDEEANDPRCIAAFLARDHGFLEAIIAEPTRCEA
                     VLAHRGISSVLLKFRGEAGHASGAQAMTANALHQAMRWGGNALDLVEAEAGACFGGLA
                     GLRFNIGKVEGGIKANIIAPSAEVRFGFRPLPSHDIDALHLRFGACATAGALERYEET
                     FRGPSLPAGDTASAEERRLLARDVADGLGLPIGNAVDFWTEASLFSQAGLTAFVYGPG
                     DIAQAHAADEYVMLEQLQRYADSIVRILGNA"
     gene            7976..>8505
                     /locus_tag="PK818_06860"
     CDS             7976..>8505
                     /locus_tag="PK818_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017174254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82488.1"
                     /translation="MGGAKEISQYLKRFSQLDAARFAVVKVGGAVLRDDLDSLTSSLA
                     FLQDVGLTPIVIHGAGPQLDAELAAAGIAKQTIDGLRVTSPEALAIVRRVFHAQNLKL
                     VEALQAGDARATSIVSGVFEADYLDRERFGLVGAVKRVDLAPIQASLQAGSIPVIASL
                     GETVGGQILNVNADFAA"
BASE COUNT         1398 a         2940 c         2942 g         1225 t
ORIGIN      
        1 gcgaagcccg caccagcgaa ggccacgcct gcagcgaagg tcgccaagcc ggccgttgcg
       61 cccgcgcggc cggtggtggt cccgccgccg ccgcatccgc gcaagctcgg caaggtcgcg
      121 gtggcagtgt cggtggccgc gcgcacgtcg gcgccggtgc cgaagggcaa gtacaaggtc
      181 gtgccgtacc agaccgacga tgccagcggc cgcccgatca tcccccccgg ctacaggccg
      241 tccgcggacg aggagtacat gagcccgctg cagctggaat atttccggca gatgctgctg
      301 gtgcgccgcg cggaactggt cgatgaatcc aagcagacca tcgagaacct caaggaagaa
      361 gtgcgcgacg tcggcgacga agccgaacgc gctacccgcg agaccgagaa ctcgctggaa
      421 ctgcgcaccc gcgaccgcta ccgcaagctg atcggcaaga tcgacagcac cctcaagcgg
      481 gtcgatggcg gcgactacgg ctactgcgtg gataccggcg aggaaatcgg cctggaccgc
      541 ctcgaggcgc gtccgcaggc ggagcgcacg atcgacgcgc aggagcgctg ggagcacctg
      601 cagaagcaga tgggcgactg agcctgccgt gttgcggtgc ccgggaaccc cgctgcggcg
      661 gggtttcccg ttactggagc tcgcgcaggt ccagcctgcg cagcttcggt gcctttgcgg
      721 cggtgaaggc ggccacgccc atggtcacga atccgcccag caccacgccc ggcaccagtc
      781 ccagcaggcg cgccatcgaa ccggcgtaga acgcgccgag ctcattcgac gagctgatga
      841 agatgctgtt gaccgaagac acccggccgc gcatcgcgtc cggggtagcc agctgcagga
      901 tggtggtgcg cagcaccacc gaaaccccgt cgaacacgcc cgacagcagc aggaacagcg
      961 cggacaacca gaacacctgg ctcagcccga agccgaccat gcacgcgccg aagccgacca
     1021 ccgcggccag caggatgcgg ccggcgttgg cctgcagcgg atgccgcgac agccacaccg
     1081 ccaccgccac cgagcccagc gcgggcgcgc cgcgcaggat ccccagcgcc tccgggccgg
     1141 cgtgcagcac atccttgatg aaggccggcg ccagcgagat cgcgccgccg aacaggaccg
     1201 cgaacatgtc cagcgccagc gcggccagca ggacctggtg gccgaacacg aagcgcaggc
     1261 cttcgccgat gctggccagc accgggccgc gctgcagttg cggggcgggc tcggtgaccg
     1321 gcattcgcag cagcgccagc gcggcggcga tcgcgaaccc cgccgccagc gcatacgcca
     1381 ccgacttgcc ggcggcgccc accagcagac cgccgatcgc cgggcccagc accagcccgg
     1441 cctggaacac cacgctgccc atgccggcgc cgcgcgcgaa ctgctcgcgc ttgagtgcgc
     1501 gcgcgaacag cgcgttgtag accgggccga ggaaggagcg gccgacgccg cccaccgcga
     1561 tcgccgcata gatcggccag gtcccgtccg ttgccagccc cccggaggcg accagcgcca
     1621 gcaccccggc attcaccgcc agcaccgtgc atgcggcggc gcccagcttg cgtcgcggca
     1681 ggtggtcgac caggtagccg gagaacggcg ccacgcagaa gaacgggatc acctcggcca
     1741 acccgatcag gcccagcgac cacgggtcgc cggtgatttc gtagacatgc cagcccaccg
     1801 tcaccgcgac cacctggtag gagagcaggg tcaggatgcg gtacagcaac agccagcgga
     1861 aacccgcggc ctgcagcggg gacgcggcgg cttcggtcat gccgccggcg cggtgcgtgc
     1921 gtgttcgcgg atgaaggcca gcagcgcggc aaggccgttg ctgcgcgtcg gcgacaggtg
     1981 ccgggcaagc ccgatcgcgg cgatgtagtc gggctcggtg gcgaggatct cggcggcact
     2041 tcgccccgaa tagacacgca gggccaggaa caccagcccg gagacgatcg ccgaatcgct
     2101 ggcggcagtg aagtcgagcc gcgacgcatc gccggaggcg accacccaca ccagcgactg
     2161 gcagccgtgc aggcggtgct gctcgacctt gcagtcgtcg gggaggtgcg gcagcttgcg
     2221 gccgaggtcg atcaggtact ggtaacgctc cgaccagtca ccgaagaacg cgaattcctc
     2281 ggcgatcgcg gcctgcgcgt cggcggcggt gggttcgagc gggaacaccg tgccgctcat
     2341 gcgcgcttcc agcgcacgcc ttgcggcgtg tcttccagca ggatgccctc gtcggccagt
     2401 tgctggcgga tcgcgtcggc gcgggcgaag tcgcgcgact tcttcgcttc gatgcgttcg
     2461 tcgatcaggc cctggatgcg ggcgtcgtcg tcgttgcccg cgccgcgcgc gaaccagtcc
     2521 gccggcgcct gctgaagcag gccgagcacg cgcccggcac cgagcagttc cgtcttcagc
     2581 cgggcctttt cggcagccga atccgccctg cgcgcctcgc cggcgatgcg cgcgatctcg
     2641 gccagcgcct gcggcgtgtt gaggtcgtca tcgagcgcgg cttcgatgct gccgggaatc
     2701 gcgacgtcgg cctcgacctc ggccaggtcg cgcagggtgc cgtacagccg gtccagcgtg
     2761 cgcaccgact gctcgatcag cccatccgac cattccagcg gctgccggta gtgcgcagag
     2821 agcagcgcgt agcgcagcgc ttccggcggg tgctggcgca ccaggtcgtg cacgcgctgg
     2881 atgttgccga ccgacttggc catcttggcg ccaccgaagt tcagcatgcc gttgtgcagc
     2941 cagaagcgcg cgaacggtgc gccgccgtgc gcgcattcgc tctgcgccac ttcgttctcg
     3001 tggtgcggga actgcaggtc cacgccgccg gcgtggatgt cgatggtttc gcccaggtgg
     3061 gcctcggcca tcgccgagca ctcgatatgc cagccggggc ggccccggcc ccagggcgag
     3121 tcccagccgg gcaggtcgtc gccggacggc ttccacagca cgaaatcgcc ggcatcacgc
     3181 ttgtaagggg ccacttcgac gcgcgcgccg gccagcattt cctcggtgtc gcggcgcgac
     3241 agcttgccgt agccgtcgaa gctcgatacc gcgaacagca cgtggccttc cgctgcatag
     3301 gcgtgtccat cggcgatcag gcgttcgatc atcgcgatca tctgcggcat gtgcgcggtg
     3361 gccgcaggct cgatgtccgg ggcgaattca cccgagaccc ccagcgcggc catgtcgtcg
     3421 cgataggcgg cggcgtactg gtcggtgatc gtggcgatcg gcacgccgag gtcccgcgcc
     3481 gcggcgttga tcttgtcgtc cacgtcggtg atgttgcggg cgtagcgcag gccgccgaaa
     3541 cgccgccgca gcagcgcggc cagcaccccg aacaccaccg gcccgcgggc gttgccgatg
     3601 tgcacgtagt tgtagacggt ggggccgcag acgtacatcg tcgggcaggc ggcatcgagc
     3661 ggtgcgaacg gttcgagccg gcgggtcagg gtgttgtgga gatgcaacct gctggaagac
     3721 gggttcatgg cgtcacgcgc gtgggatgcc gcattgtagc gggccgcaca gcggcgattc
     3781 atccgccggc agcatcgcgc cgatagactg gcgcgatgct gcgccttgcc ctgtgttgcc
     3841 tgttcgtgcc cgttgccggc ctgctggtgg cgccgcgcgt gcacgcgcag gaggtcgtgc
     3901 gcgagaccgt ggtcccgcag gaaaacgtgc gcttcgacta cgcccaggtg ctggccgtca
     3961 gtccggttta ccagaccctg cgcgccaccc gcatggagcg ggtctgcgat gccaggcagc
     4021 cttccggcaa cggcctgtcg aaggtcgtca gcgcggtcaa ggacaggctg gatggcgcga
     4081 ccagcaagga agagcgggag gccggcctga gcaactgccg gatggcaccg gtggtgcgcg
     4141 agtaccgccg gccgatcgcc tacgacgtcg attacgccta caagggcagc aagttcagga
     4201 cccggctggc gcaggacccg ggcaaccgcc tgcgggtgcg ggtctcgatc actccgcaac
     4261 cgatggcagg cccgtgaccg gcgccgggga gttgcatgct gcagcgcggc atgcgagcat
     4321 cgcggccaga tgaacgccca cctcgccacg cgccacgcag actccgcccg gatggcctcc
     4381 ggcatgacct cgcgcgcgcg ccgaccggaa tcccgacatc ccgctgggta atccggatcg
     4441 catgcgtgcg cgtcaccttc cgaaacccag cccgccggct gggttttttc gtttccggcc
     4501 accgcaaacc tccccacccg aggcactccg aatgaagcac ttcctcaata cccaggactg
     4561 gcagcgcgcc gagctcgatg cgttgctggc ggatgccgcg cgtttcaagc gggagccgct
     4621 gggggatgcg ctgaaaggcc gctcgatcgc gctggtgttc ttcaacccgt cgatgcgcac
     4681 ccgcaccagc ttcgagctgg gcgcgttcca gctgggcggg catgccatcg tgctgcagcc
     4741 gggcaaggac gcctggccga tcgaattcga cctcggcacg gtgatggacg gcgacaccga
     4801 agagcacatc gcggaagtgg cgcgggtgct ggggcgctac gtggacctga tcggcgtgcg
     4861 cgcgttcccg aagttcgtcg actggcagcg cgaccgcgag gacatcgtcc tgaagtcctt
     4921 tgcgaagtat tcgccggtgc cggtgatcaa catggaaacc atcacccatc cctgccagga
     4981 gctggcgcac gcgctggcgc tgcaggaacg cttcggtacc agcgacctgc gcggcaagaa
     5041 gtatgtgctg acctggacct accacccgaa gccgctgaac accgcggtgg ccaattcggc
     5101 gctgaccatc gccacccgca tgggcatgga cgtgaccctg ctgtgcccga ccccggacta
     5161 cgtgctggac cagcgctaca tgggctgggc cgcgcagaac gtggcggaaa gcggcggctc
     5221 gctccaggtc agtcacgaca tcgagagcgc ctacgccggc gccgacgtgg tctatgccaa
     5281 gagctggggc gcgctgccgt tcttcggcaa ctgggagccg gagaagccca tccgcgaccg
     5341 ctacaagcac ttcatcgtcg atgaagccaa gatggcgctg accaatgacg gcgtcttttc
     5401 gcactgcctg ccgttgcgcc gcaacgtaaa ggccaccgac gcggtgatgg attcgccgcg
     5461 ctgcgtcgcc atcgacgaag ccgagaaccg cctgcacgtg cagaaggcga tcatggccgc
     5521 gctcatccag caataagaca gccacccaca cagcaaccct ttcccgccag cgggcgaggg
     5581 ggagacgcaa atgactgcat ccgattcgaa agacatcgtc ctcgccttct ccggcggact
     5641 cgacaccagc ttctgcgtgc cctacctgca ggagcagggc tggaacgtgc acaccgtgtt
     5701 cgccgatacc ggcggcgtgg atgccgagga acgcgccacc atcgaggccc gtgccgaaga
     5761 gctgggcgtg gcctcgcacg tgaccgtcga cggcggtccg gcgatctggt ccggcttcgt
     5821 gaagccgttc gtgtgggcgg gcgagggcta ccagggccag tacccgctgc tggtctccga
     5881 ccgttacctg atcgtggacg ccagcctggc gcgcgcccgc gcgctgggca ccaacgcgat
     5941 cgcgcatggc tgcaccggca tgggcaacga ccaggtgcgg ttcgacctcg ccgtgaaggc
     6001 gcagggcgac taccgcatcg tggcgccgat ccgcgagatc cagaagcagc acacccagac
     6061 ccgtgccttt gagcaggcct acctggagga gcgcggcttc ggcgtgcgtg ccaagcagaa
     6121 ggcctacacc atcaacgaga acctgctggg cctgaccatg tccggcggcg agatcgacgc
     6181 ctgggaggca ccgggcgacg gcgcccgcgg ctggtgcgcg cgccgcgagc aatggccggc
     6241 cgatgcgctg gaagtgacgc tgcgcttcga caacggcgaa gcggtggcgc tggacgggca
     6301 ggcgctggcg ggcgagaaga tcctcgccaa gctcaacacc atgttcgccc agtacggcgt
     6361 cggccgcggc atgtacaccg gcgacaccac catcggcctg aagggccgca tcgtgttcga
     6421 ggcgccaggc ctggctgcgc tgctggccgc gcatcgcgcg ctggaggagg cggtgctgac
     6481 caagcagcag aaccgctgca agccggaggt ggcgcgcaag tgggtggagc tggtgtacga
     6541 aggcttctac cacgacccgc tgaagaccga cctcgaggcc ttccttgcat cctcgcagcg
     6601 gatggtcaac ggcgaggtgg tgctgcgcac cagtggcggc cgcgtcgatg ccgtcgcggt
     6661 gcgctcgccg cacatcctca actcgaaggg cgcgacctac gcgcagtccg ccgactgggg
     6721 cgtcgaggag gccgaaggct tcatcaagct gttcggcatg agcagtaccc tgtgggcaca
     6781 ggtcaacggc gggcagccgg atgcctgaat tgctgcccga tgtcctgcgc cacctcgaga
     6841 acctggtggc gttcgacacc cgcaacccgc cgcgcctgat cggcaccggc gggatcttcg
     6901 actacctgcg cgcacagctg cctggattcc gctgcgaggt cgtcgaccat ggcgacggcg
     6961 cggtgtcgct gctggccgtg cgcggcaatc cgcgacgggt gttcaacgtg cacctcgaca
     7021 cggtgccgtc ctcggaggcg tggagtgcgg atccgcacgt gctgcgggtc acccatgacc
     7081 gtgcgatcgg ccttggcgcc tgcgacatca agggcgcggc ggcagcgctg gtcgccgcgg
     7141 cgaacgcgat gaacggcgat gcggccttcc tgttctcctc ggacgaggaa gccaatgatc
     7201 cgcgctgcat cgccgccttc ctggcgcgcg accacggctt cctggaggcg atcatcgccg
     7261 agcccacgcg ctgcgaggcg gtgctggcgc atcgcggcat cagctcggtg ctgctgaagt
     7321 tcaggggcga ggcgggccat gcctccggtg cgcaggcgat gaccgcgaat gcgttgcacc
     7381 aggcgatgcg ctggggcggc aacgcgctcg acctggtcga ggccgaggcc ggtgcctgtt
     7441 tcggcgggct ggcaggcctg cgcttcaaca tcggcaaggt cgagggcggg atcaaggcga
     7501 acatcatcgc gccgagcgcc gaggtccgtt tcggtttccg cccgctgccc tcgcacgaca
     7561 tcgacgcctt gcacctgcgc tttggcgcct gcgccacggc cggtgcgctg gaacgctatg
     7621 aggaaacctt ccgcgggcca tcgttgcctg caggagacac cgccagcgcg gaggaacgcc
     7681 ggctgctggc gcgcgacgtc gccgacgggc tcggcctgcc gatcggcaac gcggtcgatt
     7741 tctggaccga ggcctcgctg ttctcgcagg ccggcctgac cgcattcgtg tatggcccgg
     7801 gcgacatcgc ccaggcccac gccgccgacg aatacgtgat gctcgaacaa ctgcagcgtt
     7861 acgccgattc catcgttcgt atcctcggga acgcctgacc gtgacgccat tgctgaaccc
     7921 ctcgcacgta catgacccgc agacgcggca gaccatcgtc cgcctgctct ccagcatggg
     7981 cggcgccaag gaaatctcgc agtacctgaa gcgcttctcg cagctggacg cggcgcgctt
     8041 cgcggtggtc aaggtcggtg gcgcggtgct gcgcgacgac ctcgattcgc tgacctcgtc
     8101 gctggcgttc ctgcaggacg tgggcctgac accgatcgtg atccatggcg caggcccgca
     8161 gttggacgca gagctggccg ccgccggcat cgccaagcag accatcgacg gcttgcgggt
     8221 gacctcgcct gaagcactcg cgatcgtgcg gcgcgtattc cacgcgcaga acctgaagct
     8281 ggtcgaggcg ttgcaggccg gcgacgcgcg ggcgacctcg atcgtctccg gcgtgttcga
     8341 agcggattac ctggaccgcg agcgcttcgg cctggtcggc gcggtgaagc gggtcgacct
     8401 cgccccgatc caggccagcc tgcaggccgg ctcgatcccg gtgatcgcct cgctcggcga
     8461 gaccgtcggc gggcagatcc tcaacgtcaa tgcggatttc gccgc
//
LOCUS       DAORUK010000084         8502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_9166_length_8502_cov_19.625192, whole genome shotgun sequence.
ACCESSION   DAORUK010000084 DAORUK010000000
VERSION     DAORUK010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8502
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9166_length_8502_cov_19.625192"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..554)
                     /locus_tag="PK818_06865"
     CDS             complement(60..554)
                     /locus_tag="PK818_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82489.1"
                     /translation="MRELERQRRNAQYRYQQDYYRRWLAQQSRWNASRYDYYSDPYYY
                     TAASYRYGYGGNWYSTNQYGANLLRQAVRDGYQEGYYAGRADRMDRWRFDYRNNYGYI
                     DGTFGYNGYYVNRNDYRYYFRQGFERGYRDGYYGRYQYGRYDNGLAIILPVVLAAILG
                     FQHY"
     gene            588..1370
                     /locus_tag="PK818_06870"
     CDS             588..1370
                     /locus_tag="PK818_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82490.1"
                     /translation="MALPFDRLAALLFDPVLLLARRAVVAVPAVAIVAAAVVAIPPRL
                     AVARFGDDALACARLRGLAAQLVGVVFGLDGLFRLALDRLATLLGDAPAGILALADLL
                     GDLLAAVGGARGVGIAKPGRALGLVPALLLDPLAHPSALADLFDVQRITPLLLFLLRG
                     ITPVLVAFRRGGACLRRLRGLGLALLPALFVLLLARGAEIVAVVIAATLLRAGGCDAA
                     DREQRAQQCGGQGADGGKAVHLVVSSWRDRFAPDACSWRLHG"
     gene            1522..3267
                     /locus_tag="PK818_06875"
     CDS             1522..3267
                     /locus_tag="PK818_06875"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQY82491.1"
                     /translation="MLPVQRLLAHALWLSSAAFAVQAGQPALTATTSATGLPRASGQE
                     VLVFERAELSFRVDPARRWLDGDATLTFVSGGTVDALFVDLDRNYRIASVEVDGQAVA
                     AGRWSNPEGRMRVDLPRPLAAGARATLRIRYAGQPHVANKAPWDGGFVWANAPTGEPW
                     VATAVQGEGCDLLWPCIDHPLGEPQEVVQHITVPSPLVAAGNGIAEGMDERDGWRTWH
                     WRAKHPNTYAIALNIGPYELLQGDYSSRYGNTIPLRFWYLKGNKAKAEGLFAELTPML
                     DFFEEMIGPFPFAGEKVGVVETPHLGMEHQTINAYGNDYRKADSGYDWLLQHEFAHEW
                     FGNQLTHRDWDDFWLHEGFGSYMQPLYAQWLRGDMDYMAALMKQRAALVNKYPVISGR
                     GLPEYEVYAAGQGPGNDTYYKGSLMLHTLRGLIGDDAFFRATRELVYGTDAPRPGNFM
                     PRHASTHDFIAIVNRLAGRDLRWFFDGYLRSAALPELLATRDADGLVLRWKTAGDQPF
                     PLPVQVRVGDRIVDVAMEDGRGRVGLPAGASYTLDPHSRVLRDLPHIAEFQRDTADRA
                     KAAAERARVEAAKKP"
     gene            complement(3312..4556)
                     /locus_tag="PK818_06880"
     CDS             complement(3312..4556)
                     /locus_tag="PK818_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HQY82492.1"
                     /translation="MRDTPAPLRLAVIGGGPAGLMAAEVASAAGIEVHLFEAKGSVGR
                     KFLIAGKGGLNLTHSEARPGFDARYRERALDVGAWLDDFDADALRAWARDFGVETYVG
                     SSGRVFPIDRKAAPLLRGWVRRLREGGVLFHVQHRWLGWDDRGALRFSTPDGEVRFGA
                     DALVLAMGGGSWPQLGSDGAWVPALAARGIDVAPLKPANCGFDIGWSAFLAKRHAGAP
                     LKPVVAHWRDAAGRERALQGECVLTENGIEGSLVYAIAADLRDAIERDGDTVLHLDLV
                     PGRDLQRLQQDLAKPRNGRSLGEHLRRQAGIDGVKSALLHEVLDKPALQDMTRVASIL
                     KRLPLRLLRARPMDEAISTAGGVRLAALDEGLMLRGLPGVFCAGEMLDWEAPTGGYLL
                     TACFASGLRAGRGAVEWLRTRG"
     gene            4610..5386
                     /locus_tag="PK818_06885"
     CDS             4610..5386
                     /locus_tag="PK818_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylargininase"
                     /protein_id="HQY82493.1"
                     /translation="MWTAITREVSPALAACELSFVPRAPIDVGRAQAQHDAYCAVLES
                     LGCGVIRLPAEPALPDSVFVEDVALAFDEVAVATRPGAESRRDEGHAVLEVLGSVRRI
                     LRIEAPGTLDGGDVLRIGKRVFVGISARSNAAGREQLCVLLAPFGYTVEGVATRDCLH
                     LKSAVTQVADDVVLVNPAWVDVAAFADYRMLEVDPAEEHAANAVRIGDVLVYPDCFPR
                     TLERLRAAGIQVATVDVSELQKAEGAVTCCSVLLKAGPVG"
     gene            5508..5660
                     /locus_tag="PK818_06890"
     CDS             5508..5660
                     /locus_tag="PK818_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82494.1"
                     /translation="MSLVGIVLLLVAIYAAFKVVGALFKTMLWIGILLFAWWYFGPRI
                     GMPFPF"
     gene            complement(5682..6875)
                     /locus_tag="PK818_06895"
     CDS             complement(5682..6875)
                     /locus_tag="PK818_06895"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY82495.1"
                     /translation="MSERAYDAIVIGAGAAGLMCAITAGQRGLRVLVLDHANKVGKKI
                     LMSGGGRCNFTNTGTTHANYLSANPHFCKSALARYTPWHFIDLVERHRIAYHEKELGQ
                     LFCDESSKLIVKMLLDECTTAGVEVRTQCTIERVEHLDDGSFRLHTAQGRLVATSLVI
                     ASGGLSIPSMGASGFGYALAKQFGHGVLPTRAGLVPLTLTGKHAERLQDLAGVSLPVE
                     ARCNGASFRNFMLVTHRGISGPSILQISSYWQPGDDLRVDLLPGIDADAQLREWQATR
                     RDAELKTLLAEVLPKRFAQRLCEHWIASKPLRQYTPPELLAVAGLLSSWPLVASGTEG
                     YRTAEVTLGGVDTDGLSSATMMSRHVPGLYFIGEVADVSGWLGGYNFQWAWASGHAAG
                     TAIAS"
     gene            complement(6872..7627)
                     /locus_tag="PK818_06900"
     CDS             complement(6872..7627)
                     /locus_tag="PK818_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HQY82496.1"
                     /translation="MPRILVFQHVAAEPLGTLDPLIRRRGHRIRFRNFERHPDALLNV
                     DRYRGLVVLGGPMNVEDHPRRPHLANEMRAIERMLEQGKPVLGICLGAQLLAHVLGAS
                     VSRNAVPEIGWYPLQKTDAGHSDPVLAPLHADTPVFQWHGCRFDIPTDAVHLARSNDC
                     DQQAFRYGDNAYGFQFHLEMDERLVERWLAQPAYRDELADSGLPHDEHAIRAQTRQHI
                     AGMQRQADAVFNNFLDLVGRPQRRYTLPSREWA"
     gene            complement(7681..>8502)
                     /locus_tag="PK818_06905"
     CDS             complement(7681..>8502)
                     /locus_tag="PK818_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HQY82497.1"
                     /translation="VLNYDIPYDTESYVHRIGRTGRAGRKGEAILFVAPRERGMLGAI
                     ERATRQKIEQMNLPSVDAVNERRVAKFLDKIEGALASDGLSVFRDLVERYEREKNVPA
                     VEIAAALAQLVQGKTPLLLARPAVPERTFEVREPRERSERPVREPRERTPRDASTPEM
                     GMTTYRIDVGYQHGVQPGNIVGAIANEADLEARFIGRIDIRDDFTLVDLPEGMPADLL
                     AHMQTVRVASRPLRMRPAEDADIDAPKARRSFGPPRGDRPGGPRKPGGFKPRPPR"
BASE COUNT         1208 a         2843 c         3035 g         1416 t
ORIGIN      
        1 cgcgggcgca agaagacgcc cggcgcgatg gccgggcgtc gaaaggtcga accggttgct
       61 cagtagtgct ggaagcccag gatcgcggcg aggacgaccg gcaggatgat ggccaggccg
      121 ttgtcgtagc gtccgtactg gtagcgaccg tagtagccgt cgcgataacc gcgctcgaag
      181 ccctggcgga agtagtagcg gtagtcgttg cggttcacgt agtagccgtt gtagccgaag
      241 gtgccgtcga tgtagccgta gttgttgcgg tagtcgaagc gccagcggtc catgcggtcg
      301 gcgcggcccg cgtagtagcc ttcctggtag ccatcgcgca cggcctggcg cagcaggttg
      361 gcgccgtact ggttggtgct gtaccagttg ccgccatagc cgtagcggta gcttgcggcg
      421 gtgtagtagt aggggtcgct gtagtagtcg tatcgcgagg cattccagcg cgactgctgc
      481 gccagccagc gacggtagta gtcctgctgg tagcggtact gggcgttgcg gcgctgccgc
      541 tccagttcgc gcacgcgctg ctcgagctgc gagcggcgcg cggattcgtg gcgttgccat
      601 tcgatcgcct ggcggcgctg ctgttcgatc cggtgctgct gctcgcgcga agagcggtcg
      661 ttgcggttcc cgcggtcgcc atcgtggcgg cggccgtcgt tgccatcccg ccacgcctcg
      721 cggttgcgcg cttcggcgat gacgcgctcg cgtgcgcccg actgcgcggg ctggcggcgc
      781 agctcgtcgg cgtggtgttc ggcctggacg ggttgttccg gctggcgctg gatcgcctcg
      841 cgacgctgct cggcgatgcg ccggctggca tcctcgcgct ggccgacctg ctcggcgacc
      901 tgctggcggc ggtcggcggc gcgcgcggcg tcggcatcgc caaaccgggt cgggccctcg
      961 gcctggtccc ggcgctgctg ctcgatccgc tggcccatcc gtccgcgctg gccgatctgt
     1021 tcgatgtcca gcgcatcacg cctttgctgc tgtttttgct gcgcggaatc accccagtgc
     1081 tcgtggcgtt ccggcgcggg ggcgcttgcc tgcggcgcct gcgcgggctc ggcctggcgc
     1141 tgctgcctgc gctgttcgta ttgctgcttg cgcgtggcgc ggagatcgtc gctgtcgtca
     1201 tcgccgccac gctgttgcgc gctggcgggt gcgatgccgc cgaccgcgag caacgtgccc
     1261 agcagtgcgg tggccagggt gcggatggcg ggaaggcggt tcatctcgtg gtctcctcgt
     1321 ggcgcgaccg attcgcgccc gatgcgtgca gttggcggct gcatggatga atcgccgctg
     1381 agattttccg gcaccgggcg gccaccgcgg attcccgcaa gccttcattc agattgcggg
     1441 caccggcggt cgtgaccaac gacaggctcg cggcctgcct gcacgtgcga aagtcccggt
     1501 ccgaccacag gaatcgcctg catgcttccc gtccagcgac tgcttgcgca cgccttgtgg
     1561 ctgtcgtccg cggccttcgc cgtgcaggcc ggacagcccg cgttgaccgc caccacctct
     1621 gccaccgggc tgccgcgcgc gtcgggccag gaggtgctgg tgttcgagcg cgccgagctt
     1681 tcgttcaggg tcgatcccgc ccgcagatgg ctggatggcg acgcaacgct gacctttgtt
     1741 tccggcggca ccgtcgatgc gctgttcgtg gacctggacc gcaactaccg gatcgcatcg
     1801 gtcgaggtgg atggccaggc ggtcgccgcg ggccgctgga gtaaccccga ggggcggatg
     1861 cgcgtggacc tgccgcggcc gctggcggct ggcgcacgcg ccaccctgcg catccgctac
     1921 gccggccagc cgcacgtggc gaacaaggcc ccgtgggatg gcgggttcgt ctgggccaac
     1981 gcaccgaccg gcgagccgtg ggtcgccacc gcggtgcagg gcgaaggctg cgacctgctg
     2041 tggccgtgca tcgatcaccc gctgggcgag ccgcaggaag tggtgcagca catcaccgtg
     2101 ccgagcccgc tggtggccgc cggcaacggc atcgccgagg gcatggacga acgcgacggc
     2161 tggcgcacct ggcactggcg cgcgaagcat ccgaacacct acgccatcgc cctcaacatc
     2221 ggcccgtatg agttgctgca gggcgactac tcatcgcgat acggcaacac gattccgttg
     2281 cgcttctggt acctgaaggg caacaaggcg aaggcggaag gcctgttcgc cgaactcacg
     2341 ccgatgctcg acttcttcga ggagatgatc ggcccgtttc cgttcgccgg cgagaaggtg
     2401 ggcgtggtcg agaccccgca cctgggcatg gagcaccaga ccatcaatgc ctatggcaac
     2461 gactacagga aggccgattc aggctacgac tggttgctgc agcacgagtt cgcccacgag
     2521 tggttcggca accagctgac ccaccgcgac tgggacgatt tctggctgca cgagggcttc
     2581 ggcagctaca tgcaaccgct gtacgcgcaa tggctgcgcg gcgacatgga ctacatggcg
     2641 gcgctgatga agcagcgcgc cgcgctggtc aacaagtacc cggtgatctc cggtcgcgga
     2701 ctgccggagt acgaggtgta cgcggcgggg caggggccgg gcaacgacac ctactacaag
     2761 ggttcgctga tgctgcacac cctgcgcggg ctgatcggcg acgacgcctt cttccgcgcg
     2821 acccgcgaac tcgtgtacgg aaccgacgcg ccacggcctg ggaatttcat gccgcgccat
     2881 gcctccacgc atgatttcat cgccatcgtc aaccgcctcg ccggcaggga tttgcgctgg
     2941 ttcttcgacg gctacctgcg gtcggcggcg ctgccggaac tgctggccac gcgcgatgcg
     3001 gatgggcttg tgctgcgctg gaagaccgcg ggcgaccagc ctttcccgct gccggtgcag
     3061 gtgcgcgtgg gtgaccggat cgtcgatgtc gcgatggagg atggtcgtgg ccgggtaggg
     3121 ttgccggcgg gcgcgagcta cacgctggat ccgcattcgc gcgtgctgcg cgacctgccg
     3181 cacatcgccg agttccagcg agacaccgct gatcgcgcga aggcggcggc ggagagggcg
     3241 agggtcgagg cggcaaagaa gccttgagtc gccgagtggc gcgaaaaggg gccccttttc
     3301 gatgccgagg ctcagccccg cgtccgcaac cactccaccg cgccgcgtcc cgcgcgcagg
     3361 ccgctggcga agcaggcggt gagcaggtag ccaccggtcg gtgcttccca gtccagcatt
     3421 tcgcctgcgc agaacacgcc gggcaggccg cgcagcatca ggccttcgtc cagggctgca
     3481 aggcgcacgc cgccggcggt ggagatcgcc tcgtccatcg gccgcgcgcg cagcagacgc
     3541 agcggcagcc gcttgaggat gctggcgacc cgggtcatgt cctgcagtgc cggcttgtcc
     3601 agcacttcgt gcaacaaggc ggatttgacg ccatcgatgc cagcctgccg gcgcaggtgt
     3661 tcgccaaggc tgcggccatt gcgtggcttg gcgaggtcct gctgcaggcg ttgcaggtcg
     3721 cggccgggta cgaggtcgag atgcaacacc gtgtcgccat cgcgttcgat cgcgtcgcgc
     3781 aggtccgcgg cgatcgcata gaccaggctg ccttcgatgc cgttctccgt cagcacgcac
     3841 tcgccctgca gcgcacgttc gcggccagcg gcatcccgcc agtgcgccac caccggcttg
     3901 agcggcgcac ccgcatggcg cttcgccagg aacgcgctcc agccgatatc gaagccgcag
     3961 ttggccggct tcagtggtgc cacgtcgatc ccgcgcgccg ccagcgcagg gacccaggcg
     4021 ccgtcggagc cgagctgcgg ccagctgccg ccgcccatcg ccagcaccaa ggcatcggcg
     4081 ccgaaccgga cctcgccatc cggcgtggag aatcgcagcg cgccccggtc atcccagccc
     4141 agccagcgat gctgcacatg gaatagcacg ccgccctcgc gcagtcggcg cacccagcct
     4201 cgcagcagcg gtgccgcctt gcggtcgatc gggaataccc ggccggaact gccgacgtag
     4261 gtctcgacgc cgaagtcgcg cgcccatgca cgcaaggcat cggcatcgaa atcgtccagc
     4321 catgccccga cgtccagcgc acgctcgcgg tagcgcgcat cgaagccggg ccgcgcttcg
     4381 gaatgggtga ggttgagccc gcccttgccc gcgatcagga acttgcgccc caccgagccc
     4441 ttggcctcga acaggtggac ctcgatgccg gccgcggacg cgacctcggc ggccatcagg
     4501 ccggcagggc cgccgccgat gacggcgagg cggagcgggg cgggtgtgtc gcgcatgcac
     4561 gcatgatccc atgcgcagcc cgctgcaatc gccggcttac aatggcgcca tgtggaccgc
     4621 gatcacccgt gaggtcagcc ccgcgctggc cgcctgcgag ctttccttcg tgccgcgcgc
     4681 gccgatcgat gtcgggcgtg cgcaggcgca gcacgacgcc tactgcgcgg tgctggaatc
     4741 gctcggctgc ggggtgatcc ggctgccggc cgagccggcg ctgccggatt cggtgttcgt
     4801 ggaagacgtg gcgctggcct tcgatgaggt cgcggtggcc acccgcccgg gtgcggagtc
     4861 ccgtcgcgac gagggccatg cggtgctcga ggtgctcggc agtgtccgcc gcatcctgcg
     4921 catcgaagcc cccggcacgc tggatggtgg agacgtcctg cgcatcggca agcgtgtgtt
     4981 cgtcggcatc tccgcgcgca gcaacgccgc gggcagggag cagctgtgcg tgctgctggc
     5041 gccgttcggc tacacggtgg aaggggtcgc cacccgcgac tgcctgcacc tgaagtcggc
     5101 ggtgacccag gtggcggacg acgtggtgct ggtcaacccg gcctgggtcg atgtcgccgc
     5161 gttcgcggat taccggatgc tcgaggtgga tcccgccgag gagcacgccg ccaacgcggt
     5221 gcgcatcggc gatgtgctgg tctatccgga ctgcttcccg cggacgctgg agcgcctgcg
     5281 ggcggccggc atccaggtcg ccacggtcga cgtgtccgag ctgcagaagg cggaaggcgc
     5341 ggtgacctgt tgcagcgtgc tgttgaaggc aggaccggtg gggtgacgtt tgccgatgcc
     5401 gttgccggtg ctctcgtcgt ccgggagaga gccgcggcgt caccgcggga atgcgcgagc
     5461 atcgccgctc gcctaagctg cacaacttca ggacagggaa gtcggaaatg agtctggtcg
     5521 ggatcgtgtt gctgctggtg gcgatctacg ctgcgttcaa ggtggtcggg gcgttgttca
     5581 agaccatgct gtggatcggc atcctgctgt tcgcctggtg gtacttcggc ccgcgcatcg
     5641 gcatgccgtt tccgttctga cgccgcctgg cgccgcgatc gtcacgaagc gatcgcggtg
     5701 cccgccgcgt ggccgctggc ccaggcccac tggaagttgt agccgcccag ccagccgctg
     5761 acatccgcca cctcgccgat gaaatacagg cccgggacat gccgcgacat catcgtggcc
     5821 gacgacaggc cgtcggtatc gacgccaccg agggtcactt ccgcggtgcg gtagccctcg
     5881 gtgccgctgg cgaccagcgg ccatgacgac aacagcccgg caacggcgag cagttccggc
     5941 ggggtgtatt gccgcaacgg cttgctggcg atccagtgct cgcacaggcg ttgggcgaag
     6001 cgcttcggca ggacttcggc cagcagcgtc ttcagttctg catcgcggcg ggtcgcctgc
     6061 cattcgcgca gctgcgcgtc ggcatcgatg cctggcaaca gatcgactcg caggtcatcg
     6121 ccgggttgcc aataggagct gatctgcagg atcgacggcc cgctgatccc gcggtgggtc
     6181 accagcatga agttgcggaa gctggcgcca ttgcagcgcg cctccaccgg cagcgatacc
     6241 ccggccaggt cctgcaggcg ctcggcatgc ttgccggtca gggtcagcgg gaccagcccg
     6301 gcgcgggtcg gcagcacccc atggccgaac tgcttcgcca gtgcgtaacc gaagccgctc
     6361 gcgcccatgc tcgggatcga caggccgcca ctggcgatca ccagtgacgt cgcaacgagc
     6421 cggccctgcg cggtgtgcag gcggaaactg ccatcgtcga gatgctcgac gcgctcgatc
     6481 gtgcattgcg tgcggacttc gacgccggcg gtcgtgcatt cgtccagcag catcttgacg
     6541 atgagcttgg aggactcgtc gcagaacagc tggccgagtt ccttctcgtg gtaggcgatg
     6601 cggtggcgtt cgaccagatc gatgaagtgc cagggcgtgt agcgcgccag cgccgacttg
     6661 cagaagtgcg gattggccga caggtagttg gcgtgggtgg tgccggtatt ggtgaaattg
     6721 cagcgcccgc cgccggacat caggatcttc ttgccgacct tgttggcgtg gtcgaggacg
     6781 agcacgcgca ggccgcgctg gccggcggtg atcgcgcaca tcagcccggc cgcgccggcg
     6841 ccgatgacga tggcgtcgta cgcgcgctcg ctcacgccca ctcgcgcgag ggcagggtgt
     6901 agcgccgttg cgggcggccg accaggtcga ggaagttgtt gaacaccgca tccgcctgcc
     6961 gttgcatccc cgcgatgtgc tggcgggtct gcgcgcggat cgcatgttca tcgtgcggca
     7021 ggccggaatc cgccagctcg tcccgatagg caggctgggc cagccagcgt tcgaccaggc
     7081 gctcgtccat ctccaggtgg aactggaagc cgtaggcgtt gtcgccgtag cggaaggcct
     7141 gctgatcgca gtcgttgctg cgtgcgaggt ggaccgcatc ggtcgggata tcgaaccggc
     7201 agccgtgcca ctggaacacc ggggtgtccg catgcaacgg cgccagcacc gggtcggagt
     7261 ggccggcatc ggtcttttgc agcggatacc agccgatttc cggcaccgcg ttgcgcgaaa
     7321 cgctcgcgcc cagcacgtgc gccagcaact gcgcgcccag gcagatcccg agcaccggct
     7381 tgccctgttc gagcatgcgt tcgatcgcgc gcatctcgtt ggccaggtgc gggcggcgtg
     7441 gatggtcttc gacgttcatc gggccgccca gcaccaccag cccgcgatag cgatcgacgt
     7501 tgagcagcgc gtccggatgc cgctcgaagt tcctgaagcg gatccggtgg ccacgccgcc
     7561 ggatcagcgg gtcgagcgta cccagcggtt cggcggcgac gtgctggaag acgaggatgc
     7621 gcggcatgtg gccactatgc caaacgccgc cgcgtcatcg ccgtgcgatg cgtcatgcgc
     7681 ttatcgcggc gggcgcggct tgaagccacc cggcttgcgc gggccaccgg ggcggtcgcc
     7741 gcgcggcggg ccgaagctgc gcctggcctt gggtgcgtcg atgtccgcgt cctcggcggg
     7801 gcgcatgcgc agtggccggc tggcgacccg cacggtctgc atgtgcgcca gcaggtcggc
     7861 cggcatgccc tcgggcaggt cgaccagggt gaagtcgtcg cggatgtcga tgcggccgat
     7921 gaaacgcgcc tccaggtcgg cctcgttggc gatcgcgccg acgatgttgc ccggctgcac
     7981 gccgtgctgg tagccgacat cgattcggta ggtggtcatg cccatttcgg gcgtgctggc
     8041 gtcgcgcggg gtgcgttcgc gcggctcgcg caccgggcgt tcgctgcgtt cgcgcggttc
     8101 acgaacctcg aaggtgcgct ccggcacggc cggcctggcc agcagcagtg gggtcttgcc
     8161 ctgcaccagt tgcgccagcg cagcggcgat ttccaccgcc ggcacgttct tctcgcgctc
     8221 gtagcgctcc accaggtcgc ggaacaccga caggccgtcg ctggccagcg caccctcgat
     8281 cttgtcgagg aacttcgcca cccggcgctc gttgacggcg tccacgctgg gcaggttcat
     8341 ctgctcgatc ttctgccggg tggcgcgctc gatcgcgccc agcatgccgc gctcgcgcgg
     8401 ggccacgaac aggatcgcct cgcccttgcg gccggcgcgg ccggtgcggc cgatgcggtg
     8461 cacgtagctc tcggtgtcgt acgggatgtc gtagttcagc ac
//
LOCUS       DAORUK010000085         8435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_9280_length_8435_cov_16.508234, whole genome shotgun sequence.
ACCESSION   DAORUK010000085 DAORUK010000000
VERSION     DAORUK010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9280_length_8435_cov_16.508234"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..381
                     /locus_tag="PK818_06910"
     CDS             235..381
                     /locus_tag="PK818_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82498.1"
                     /translation="MPIDFCTTRAALMPVAVRVVDLGLSRALRRSDSVRVNHAGALVG
                     RSCI"
     gene            complement(644..1915)
                     /locus_tag="PK818_06915"
     CDS             complement(644..1915)
                     /locus_tag="PK818_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQY82499.1"
                     /translation="MTPALPFLRWLPQWRQKDVLRADLLAGLTGAIVVLPQGVAFATL
                     AGMPPAYGLYAAMMPCVIAALFGSSRLMVTGPANAISLTTMALVAPLAAVGSSQYVGL
                     VLTLTFVVGALQLLLGMARVGKWVELVPHAVIAGFTAGAAVLIVNSQVGTLLGLDLPR
                     GLSVAQTLRVVFTTGQSIQALPVIAAMATILINLAALRRSRWIPPILMAVIGGTAITW
                     LAAGLLGAQPATVEALPGALPPLSLPDLRQVPNLVLPALVMTLLAVTEAMAIAKAFAR
                     RANEPFDGNQELVGQGLANLTGSFFSSYPASGSFNRSGVNVAAGARTPLAAVSAAVLL
                     IVILSFVAPWARWLPLAVIGGLLVVVAWGLVNPREIRHLWKHEPVDRLPMVVTFAGTV
                     TLSLEWAILLGLATAWVSRRLAGPETGSGSL"
     gene            complement(1912..2844)
                     /locus_tag="PK818_06920"
     CDS             complement(1912..2844)
                     /locus_tag="PK818_06920"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HQY82500.1"
                     /translation="MKPALSIILFTTIAGAAQGMVVALALATLAGIAPATALAPMLWA
                     ATGMLLVALVASFFHLGHPLRAWRTVLMWRTSWMSREVIVLPAFIALVGGWALLATLA
                     QRDAVAPHWLAGMAIVGAVLLWYCTAMIYACIRFIQEWAHPLTVVNYTTLGLASGFVT
                     AGALAVMAGEPGFASDIAPWALAATALAWFTRALSLRRNARLKPKSTTQTATGIQAAR
                     VVQKSMGMTGGSFNTREFFHGASAHGMRMARTGFQVLAFALPILLLAWALLSGSALAW
                     LLALGSQAAGLLAERWFFFAQARHPQNIYYQAVS"
     gene            complement(2841..3608)
                     /locus_tag="PK818_06925"
     CDS             complement(2841..3608)
                     /locus_tag="PK818_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQY82501.1"
                     /translation="MNNPVQPAPETLAKQLALVIDLNVCVGCHACVTSCKEWNTSGAA
                     GPLTDLNAYGERPTGTFFNRVQTFEADSYPDTQTIHFPKSCLHCEDPPCVPVCPTGAS
                     YKRKSDGIVLVDYDKCIGCKYCSWACPYGAREFDEERKVMTKCTLCVDRIYDELLPPQ
                     DRKPACVKACPTGARLFGDVKDPDSEVSQAIRERGGYQLMPEWETQPANHYLPRQITL
                     STVPQAQPNFRLGDFLNGEPASGKHKPGSGAMEKDAR"
     gene            complement(3605..6514)
                     /locus_tag="PK818_06930"
     CDS             complement(3605..6514)
                     /locus_tag="PK818_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HQY82502.1"
                     /translation="MFKFLSSEPVHDATASSTADTEVKTTTCYMCACRCGIRVHLRDG
                     QVRYIDGNPDHPINKGVICAKGSAGIMKQVSPARLTQPLLRRPGSERGEGEFEPISWE
                     RAYELLTERLGRIRATDPKKFALFTGRDQMQALTGLFARQFGTPNYAAHGGFCSVNMA
                     AGMIYSVGGSFWEFGGPDLDRAKLFVMIGTAEDHHSNPMKIALGDFKRKGGRFISINP
                     VRTGYSAIADEWIPIRPGTDGALFMALLHELLAQGLVDRSFLQRFTNAPQLVVLDEGE
                     REGLFAFDPAAGPPGDGRHPHNKLAWDLASGRVVQAYPEGVEEGRQLALEGHYKLADG
                     TRVAPAFQLLRDRVADTTPEWAEDITGIPSERIRQLAREVGETALHQAFELPIPWTDA
                     WGKRHPTTQGRPVAFHAMRGLAAHSNGFQTVRALAVLMSVLGTIDAPGGFRHKAPYPR
                     HIVPNYRAFNDPSMIKPNTPLNAAPLGFPAGPDELAVHEDGTPLRIDHAFSWEHPLSA
                     HGMMHNVITNAVRGDPYEIDTLLIFMANMAWNSTMNTMEVRKKLNARGEDGNYKIPFL
                     VVCDAFQSEMVAFADLVLPDTTYLERHDVMSMLDRPISEYDGPVDSVRVPVVEPTGNC
                     KPFQEVLIELASRLKFPAFTREDGTRKFKDYPDFVVNFQAQPGVGFLMGWRGKDGTEH
                     LRGDPNPRQWEMYARNNCVFQYHMPEDQQYMRNWNQPYLDFAKDKGWRQRNDPVQLAI
                     YSDTLQAFRLAAQGKSKGRQPPEHLRERIATYFDPLPFWYAPLETACIDADRYPLAAI
                     TQRPMAMYHSWDSQNAWLRQIHSHNYLYVNPRTAQEAGIEDGGWAWVTSAWGELRCML
                     RYSDAVEPGTVWTWNAIGKGSGAWQLAPAADESRKGFLLNHLITEELPFAGGHISNSD
                     PVTGQAGWYDVRVNLRPAAPEEPAETFPQIATVPSAPGVLGGARKVLAYLVPRKP"
     gene            6567..7463
                     /locus_tag="PK818_06935"
     CDS             6567..7463
                     /locus_tag="PK818_06935"
                     /inference="COORDINATES: protein motif:HMM:NF008095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQY82503.1"
                     /translation="MNQKIDQRLKLTLRQLEVFAAVARGGTTRAAADEISRSQSAASN
                     ALGELEAVLGAQLFDRVGKKLVINENGRALLPRAAGILEQAVETEALFTAAHAASLRL
                     AASYTIGEYLLPVLIARWKQANPRSHVLLDIANTHDVFERVASFRSDIGFIEGTHSHP
                     ELSVSKWRSDEIVVVAAAGHPLAERRAGARQLNHATWVLREPGSGTREASDRWLIPHL
                     TQMEVELELGSNEAVKRVVAAGLGLGCLSRLAVKEAIAEGWLVELATTLPPMQRTLSI
                     VLHRAKKLGAAAEGFLRHCTTD"
     gene            7549..7959
                     /locus_tag="PK818_06940"
     CDS             7549..7959
                     /locus_tag="PK818_06940"
                     /inference="COORDINATES: protein motif:HMM:NF021192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase associated protein"
                     /protein_id="HQY82504.1"
                     /translation="MRPDVGPTACLSFEPDEARRLLWLPLAVRHKLDGCGLRLSLLEW
                     QALPLDTRAELLHLPAGADFALCALHAGASRDTRLRQPVRLEAYEVAQALDCDAAAAG
                     AWLAAATPFAHYVLSRRNRAETWVAAGGVGLAAR"
     gene            complement(8177..>8435)
                     /locus_tag="PK818_06945"
     CDS             complement(8177..>8435)
                     /locus_tag="PK818_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82505.1"
                     /translation="EALRATLVAALTTMSVMRIVVVLATGQFSLLALKMILLAAPAVL
                     GTSWLLRRYPPRWRRETVLRIVCVLLLATGVSIAASALLGR"
BASE COUNT         1383 a         2835 c         2889 g         1328 t
ORIGIN      
        1 cagtgctagc ttttcgtacc gtcacttatt gcactgaaaa cgaggagacc ccaagaagaa
       61 ccagcgctcg gccagcaggc cggccgcctg cgaggccagc gccagcagcc tggccagcgc
      121 cgagccggac agcagcgccc aggcgagcag ccctggaagc ccacccgggc cacgcgcact
      181 gcgggggccg gggcgccgtg gaagaactcg cgcgtgttga acgagccgcc ggtcatgccc
      241 atcgacttct gcaccacccg cgccgcattg atgccggtgg cggtccgcgt ggtggacttg
      301 ggcttgagcc gcgcattgcg gcgcagcgac agcgtgcggg tgaaccacgc cggtgctctc
      361 gttggccgaa gttgcatctg acgcgcatct cgttgcgcgc ggcaatttca cggttgctgg
      421 tggtgctctc cagctacaga ccgcgcctcg gttttagccg gtaaatcgca aaaccggacg
      481 cgtctagatg aacaaagccc cggtcggctg ttcagcgttt tactgccgga cgcggtactg
      541 gataagccgc agggccatgc gatgcgggac gccgggctgc gggaatcaag cagctcctgc
      601 cggcggccgt gaagtacgtg tccgcggccg agttgcctcc cccctacaag ctcccactcc
      661 ccgtctccgg gcccgccagc cgccgcgaca cccaggccgt cgccagtccg aggaggatcg
      721 cccactccag cgacagcgtc accgtcccgg cgaaggtcac gaccatcggc agccggtcga
      781 ccggctcatg cttccatagg tgccggatct cgcgcgggtt caccagcccc cacgccacga
      841 ccaccagcag ccccccgatt accgccagcg gaagccagcg cgcccagggc gcgacaaagg
      901 acaggatcac gatcagcagc acggcggcgc tgaccgccgc cagcggcgtg cgggcgccgg
      961 ccgccacgtt cacgccggag cggttgaatg aaccgctggc tggatacgat gagaagaagg
     1021 agccggtcag gttggccagc ccctggccga ccagttcctg gttgccgtcg aaaggctcgt
     1081 tggcgcgccg cgcgaacgcc ttggcgatcg ccatcgcctc ggtcaccgcc agcagcgtca
     1141 tcaccagcgc cggcagcacc aggttcggca cctggcgcaa gtcgggcagc gacagcggcg
     1201 gcagcgcccc cggcagcgcc tccacggtgg ccggctgcgc gcccagcagg ccggcggcga
     1261 gccaggtgat ggcggtgccg ccgatcactg ccatcaggat cggcggtatc cagcggctgc
     1321 gccgcagcgc cgccaaattg atcaggatgg tggccatggc ggcgatcacc ggtagcgcct
     1381 ggatggactg cccggtcgtg aataccaccc gcagcgtctg cgccaccgat agcccgcgcg
     1441 gcaggtcgag tccgagcagc gtgccgactt ggctgttgac gatcagcacg gcggcgccgg
     1501 cggtgaagcc ggcgatcacc gcatgcggca ccagctcaac ccacttgccg acgcgcgcca
     1561 tccccagcag caactgcagc gcccccacca cgaacgtgag cgtgagcacc aggcccacgt
     1621 actgcgaact gcccaccgcc gccagcggtg ccaccagcgc catggtggtg agcgagatgg
     1681 cattggccgg ccccgtcacc atcaggcggc tgctgccgaa cagcgcggcg atcacgcacg
     1741 gcatcatcgc cgcatagagg ccgtaggcgg gcggcatgcc cgccagcgtg gcgaaggcca
     1801 cgccctgggg cagcaccacg atggccccgg tcaggccggc cagcaggtcc gcccgcagca
     1861 cgtccttctg ccgccactgc ggcagccagc gcaagaaggg cagcgccggc gtcacgacac
     1921 tgcctggtag tagatattct gcggatgccg tgcctgcgca aagaagaacc agcgttccgc
     1981 cagcaggcct gccgcctgag atcccagcgc cagcagccac gccagcgccg agccgctcag
     2041 cagtgcccag gcaagcagca ggatcggcag tgcgaaggcc agcacctgaa agcctgtccg
     2101 cgccatgcgc attccgtgtg ccgaagcccc atggaagaac tcgcgcgtat tgaaggagcc
     2161 tcccgtcatg cccatggact tctgcaccac ccgcgccgcc tggatgccgg tcgccgtctg
     2221 ggtggtggac ttcggcttca gccgcgcatt gcggcgcagc gacagcgcgc gggtgaacca
     2281 cgccagcgcc gtggccgcca gcgcccaggg ggcgatgtcg gaggcgaacc cgggttcccc
     2341 ggccattacc gccagcgcgc cggcggtgac aaagccggag gcgaggccga gcgtcgtgta
     2401 gttcaccacc gtgagcggat gcgcccattc ctggatgaag cggatgcagg catagatcat
     2461 cgcggtgcag taccacagga gcacggcgcc cacgatcgcc attccggcga gccagtgcgg
     2521 cgccaccgcg tcgcgctgcg ccagtgtggc cagcagggcc cagccgccta ccagcgcgat
     2581 gaacgccggc agcacgatca cctcgcgcga catccaggag gtgcgccaca tcagcaccgt
     2641 acgccaggcg cgcagcggat gccccagatg gaagaaggag gcgaccaggg ccaccagcaa
     2701 catgccggtg gccgcccaaa gcatgggggc gagcgccgta gccggcgcga tcccggccag
     2761 agtggccaac gccagtgcca ccaccatccc ctgggcggcg ccggcgatcg tggtgaaaag
     2821 gatgatcgag agggctggct tcatcgggcg tctttttcca ttgcaccgct gcctggcttg
     2881 tgcttgccgg acgccggctc gccgttcagg aaatcgccca gccggaaatt cggctgcgcc
     2941 tgcggcaccg tgcttagggt gatctggcgc ggaaggtagt ggttggccgg ctgggtctcc
     3001 cactcaggca tcaactggta gccgccacgc tcgcggatcg cctgcgaaac ctcggagtcc
     3061 ggatccttga cgtcgccgaa caggcgggcg ccagtcggac aggccttcac gcaggccggc
     3121 ttgcggtcct gcggcggcag cagctcgtcg tagatgcggt ccacacacag ggtgcacttg
     3181 gtcatcacct tgcgttcctc gtcgaactca cgggcgccgt aggggcaggc ccagctgcaa
     3241 tatttgcagc cgatgcactt gtcgtagtcc accagcacga taccgtcgct cttgcgcttg
     3301 tagctggcac cggtagggca gacaggcacg cagggtgggt cctcgcagtg caggcagctc
     3361 ttggggaagt ggatggtctg cgtatccggg tagctatcgg cctcgaaggt ctggacccgg
     3421 ttgaagaagg tgccggtggg ccgctcgccg taagcattca ggtccgtcag cgggccggcc
     3481 gcgcccgagg tgttccactc cttgcacgag gtgacgcagg catggcagcc gacgcagaca
     3541 ttcaggtcga tgaccagagc aagctgcttg gccagtgttt cgggggctgg ctggactggg
     3601 ttgttcatgg cttcctggga acgaggtagg caagcacctt gcgggcgccc ccgagcacgc
     3661 ctggggcgct cggcacggtt gcaatctggg ggaaggtttc cgctggttct tcgggcgccg
     3721 ccgggcgcag gttcacccgc acgtcgtacc agccggcttg gccggtgacc gggtcggagt
     3781 tgctgatgtg tccaccggca aagggcagct cctcggtgat caggtgattg agcaggaagc
     3841 ccttgcgcga ctcgtcggcg gcaggcgcca actgccaggc gccggaaccc ttgccgatcg
     3901 cgttccaggt ccagaccgtg cccggctcca ccgcatcgct gtagcgcagc atgcagcgca
     3961 gctctcccca ggcgctggtc acccaggccc agccgccgtc ctcgatgcca gcttcctgcg
     4021 cggtgcgcgg gttcacgtac aggtagttgt ggctgtggat ctggcgcagc caggcgttct
     4081 gcgaatccca ggagtggtac atcgccatcg gccgctgggt gatcgccgcc agggggtagc
     4141 ggtcggcgtc gatgcaggcg gtctccagcg gcgcatacca gaacggcagc ggatcgaagt
     4201 aggtggcgat gcgctcgcgc aggtgctccg gcggctggcg gcccttgctc ttgccctgcg
     4261 cggccagccg gaaggcctgc agcgtgtccg agtagatggc cagctgcacc gggtcgttgc
     4321 gctgccgcca acccttgtcc ttggcgaagt ccaggtaagg ctggttccag ttgcgcatgt
     4381 actgctggtc ttccggcatg tggtactgga agacgcagtt gttcctggcg tacatctccc
     4441 actgccgggg gttgggatcg ccacgcaggt gctcagtgcc gtccttgccg cgccagccca
     4501 tgagaaagcc cacgcccggc tgggcctgga agttgacgac gaagtcggga tagtccttga
     4561 acttgcgcgt gccgtcctct cgggtgaagg cggggaattt caggcgcgag gccagctcga
     4621 tcagcacctc ctggaacggc ttgcagttcc cggtgggctc caccacgggg acgcgcacgg
     4681 aatccaccgg accgtcgtac tccgagatcg gccggtccag catgctcatg acgtcgtgcc
     4741 gctcgagata ggtagtgtcc ggcagcacca ggtcggcgaa ggcgaccatc tcgctctgga
     4801 aggcatcgca caccaccagg aacggaatct tgtagttgcc gtcctccccg cgcgcgttga
     4861 gcttcttgcg cacctccatg gtgttcatgg tggagttcca cgccatgttc gccatgaaga
     4921 tcaacagtgt gtcgatctcg taggggtcac cgcgcaccgc gttggtgatg acgttgtgca
     4981 tcatcccgtg ggccgacagc gggtgctccc aggagaacgc gtgatcgatg cgcagcggcg
     5041 ttccgtcctc gtgcaccgcc aattcgtcgg ggccggcggg aaaacccaat ggcgccgcgt
     5101 tcagcggcgt gttgggcttg atcatggacg ggtcgttgaa ggcccggtag ttcggcacga
     5161 tgtggcgcgg gtacggcgcc ttgtggcgga agccgccggg cgcgtcgatg gtgcccagca
     5221 cgctcatgag caccgccagc gcgcgcaccg tctggaaccc gttggaatgc gccgccagcc
     5281 cgcgcatggc gtggaaggcc accggccgcc cttgggtggt ggggtgccgc ttcccccacg
     5341 catcggtcca cggaatggga agttcgaagg cctggtgcag cgccgtctcc cccacttcgc
     5401 gcgccagctg gcggatgcgc tcggacggga tgccagtgat gtcctctgcc cactcgggcg
     5461 tggtgtcggc aacgcggtct cgcaacagct ggaaggctgg cgccacacgc gtcccgtccg
     5521 ccagcttgta gtggccctcc agcgccagtt ggcgcccttc ctccacgcct tccggatagg
     5581 cctgcaccac ccggccgctg gccaggtccc atgccagctt gttgtgcggg tggcggccgt
     5641 cgccgggcgg gccggcggcg ggatcgaagg cgaacaggcc ttcgcgctcg ccctcgtcca
     5701 gcaccaccag ttgcggcgca ttggtgaacc gctgcaggaa ggaccggtcg accaggcctt
     5761 gcgcgagcag ttcgtgcagc agcgccatga acagtgcgcc gtcggtgccg gggcggatcg
     5821 gaatccactc gtcggcgatc gccgaatagc cggtgcgcac ggggttgatc gagatgaagc
     5881 ggccgccctt gcgcttgaag tcgcccaagg cgatcttcat cgggttgctg tggtggtctt
     5941 ccgcggtgcc tatcatcacg aacagcttgg cgcgatccag gtccgggccg ccgaactccc
     6001 agaagctgcc gccgaccgaa tagatcatgc cggcagccat gttcaccgaa cagaagccgc
     6061 cgtgcgccgc atagttcggt gtgccgaact ggcgcgcgaa caggccggtg agggcctgca
     6121 tctggtcgcg cccggtgaac agggcgaact tcttcggatc ggtggcgcgg atacggccca
     6181 ggcgctcggt gagcagctcg taggcgcgct cccaggagat cggctcgaac tcaccctcgc
     6241 cccgctcgct gcctggcctg cgcagaagcg gctgcgtcag gcgcgcaggc gatacctgct
     6301 tcatgattcc cgcgctgccc ttggcgcaga tcacgccctt gttgatcggg tggtcgggat
     6361 tgccgtcgat gtaccggacc tgcccgtcgc gcaggtgcac gcgtatgccg cagcggcagg
     6421 cacacatgta gcacgtagtc gtcttgacct cggtgtccgc ggtgctgctc gcggttgcat
     6481 cgtgcaccgg ctcgctggag aggaacttga acataagctt atgctagaca gcgcacagat
     6541 tcgtgtgccg cataattccg gttgcagtga atcagaaaat cgatcagaga ctgaagctca
     6601 cgctgcgcca gctggaggtg ttcgcggcgg tggcgcgcgg cggcacgacg cgggccgcgg
     6661 ctgacgagat ctcgcgctcg cagtcggccg cgagtaacgc gctcggcgag ctggaggccg
     6721 tgcttggtgc gcagctcttc gaccgggtgg gcaagaagct ggtcatcaac gagaatgggc
     6781 gggccctgct gccgcgcgcg gccggaatcc tggagcaggc ggtggagacg gaagccctgt
     6841 tcaccgcggc ccatgcggcg tcgctgcggc tggccgccag ctacacgatc ggcgagtacc
     6901 tgctcccggt gctgatcgcc aggtggaagc aggcgaaccc gcgcagccac gtgctgctgg
     6961 atatcgccaa cacgcatgac gtgttcgagc gggtcgcctc gttccgctcc gacatcggtt
     7021 tcatcgaggg gacccacagc cacccggagc tctcggtcag caagtggcgc agcgatgaga
     7081 tcgtcgtggt cgccgccgct ggccacccgt tggcggaacg ccgcgccggc gcacgccagc
     7141 tgaaccatgc cacctgggtc ctgcgcgaac ccggctctgg cactcgggaa gcctcggacc
     7201 gctggctgat cccgcatctg acacagatgg aggtggagct cgagctgggg agcaacgagg
     7261 cggtcaagcg cgtcgtggcc gcaggactgg gcctggggtg cctgtcccgg ctggcggtga
     7321 aggaggcgat cgccgagggg tggctggtgg agcttgccac caccctgccc ccgatgcagc
     7381 gcaccctttc catcgtgctg caccgggcga agaagctggg cgccgcagcc gaagggttcc
     7441 tgcgccactg cacgacggac tgacacggcc cgaggacgat cgattattca ggtcaatgcg
     7501 acctgaatat tgcgttctcg aacttcaggc gggcgctatg ctgtccgtat gcgacccgac
     7561 gtggggccca ccgcctgcct ctccttcgaa cccgacgaag ctcgccgcct gctgtggctg
     7621 ccgctcgctg tgcgccacaa gctggacggc tgcggactgc ggctgtcgct gctcgagtgg
     7681 caagcgctgc cgctggacac gcgtgcggaa ctgctgcacc tgcctgccgg ggcggacttc
     7741 gcgctgtgcg cgctccacgc cggcgccagc cgcgacacgc gcctgcggca accggtgcgc
     7801 ctggaggctt acgaggtcgc ccaggcgctg gactgcgatg ccgccgcagc cggtgcttgg
     7861 ctcgcggctg cgacgccgtt tgcgcactat gttctcagca ggcggaatcg ggctgaaact
     7921 tgggtggccg ccggcggagt ggggctcgct gcgcggtgaa ggggacgcgt acaagcgcga
     7981 atcagtgcgg cggccgatcc caagcaacgc gaacgcgagc tggaggcgga actgagcgag
     8041 ctcacctcct cgttcctcac tgcagaagcc ttcagcgtgg aggacaatca tcgacccgcg
     8101 tgacacgcgg cggtagtgtg ccgcctcatc gatgcacgcg cagtcgcgca tgcacaggtt
     8161 cctcatgcgt gcggactcaa cgtcccagca gggctgaggc ggcgatgctg acgccggtgg
     8221 cgagaagcaa cacgcacacg atcctgagca ccgtctcgcg tcgccagcgg gggggatagc
     8281 gtcgcagcag ccaggacgtc cccagcaccg caggggcggc gagcaggatc atcttcagcg
     8341 caagcaggct gaactgtccc gttgccagga cgacgacaat ccgcatcacg ctcatcgtgg
     8401 tcagcgcggc gaccaaggtg gcccgcaggg cttcc
//
LOCUS       DAORUK010000086         8408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_9329_length_8408_cov_17.935831, whole genome shotgun sequence.
ACCESSION   DAORUK010000086 DAORUK010000000
VERSION     DAORUK010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8408
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9329_length_8408_cov_17.935831"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PK818_06950"
     CDS             complement(<1..233)
                     /locus_tag="PK818_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HQY82506.1"
                     /translation="MNPQVANLQARYPRIALVGNPNCGKTALFNLLTGSRQKVANYAG
                     VTVERKEGRLHAPSGRQYAVLDLPGAYSLNAASL"
     gene            complement(230..478)
                     /locus_tag="PK818_06955"
     CDS             complement(230..478)
                     /locus_tag="PK818_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HQY82507.1"
                     /translation="MKLNQLARRTPAIVEAVEDLAPNDAIARRLRELGFVAGEQVEVV
                     AAGPFGAEPLLVQVGFTRFALRQVEAARVRLRAGGAGA"
     gene            572..1357
                     /locus_tag="PK818_06960"
     CDS             572..1357
                     /locus_tag="PK818_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQY82508.1"
                     /translation="MSERLHLHRDGPVARLRMTRAQVHNAFDAGLIAGMTAALEDVAG
                     DAGIRVLVLEAEGASFSAGADLNWMRGMAAASEADNREDSLALARLMRTLDELPKPTI
                     ARVQGAAFGGGVGLVACCDIAIGVPEAKFGLTESRLGLLPAVISPYVINAIGPRHARR
                     YFASAEIFDAAEALRIGLLHQVVPADELDAAVRQQLALLLKAGPIASASAKSLVRAAC
                     AHADGARHDADNAALIARLRVSPEGQEGLSAFLDKRKPHWIDA"
     gene            1357..3345
                     /locus_tag="PK818_06965"
     CDS             1357..3345
                     /locus_tag="PK818_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HQY82509.1"
                     /translation="MFSKILIANRGEIACRVIRTARRLGIRTVAVFSDADADAQHVRL
                     ADEAHHIGGPRPQDSYLRGDAIIAVAQRTGAQAIHPGYGFLSENAGFADAVAAAGIAF
                     IGPSGASMRKMGSKAGAKELMHAAGVPVVPGYTGEDQSPDLLQREADAIGYPLMIKAA
                     HGGGGKGMRIVRDSAEFAPMLESCQREAANAFGRDRVLLERYVEKPRHVEIQVFGDGF
                     GNAIHLNERECSAQRRYQKVLEESPSPFLTGDLREAMGAAAVLAARAIDYVNAGTVEF
                     IVGQDGGFYFMEINTRLQVEHPVTEETTGLDLVEWQLRVAAGEPLPLAQADVPRHGHA
                     IEVRLYAEDPDAGFLPGSGTLQALRLPAPSRHVRIDSGVIEGDTVSIFYDPMIAKLIV
                     WDADRPRALARLREALAGCEIAGPKSNIGFLERLVRHPAVVDASIDTGYLDRHLDEFL
                     APAGTDITLLLAAVSVRLLAQEADACAAAGHDPGSPWAIADGWRLGHAGARRLAFADG
                     DHRIDVIAHGHGGSYRIELDGACHEVAAARLLDGTLSARIDGAMRRVGALASAERVLV
                     HDGERRLQLASVEVYRRGAEAGAGGEHRIRAPMPGRVVVVQKLPGDAVGVGDVVLVIE
                     AMKMELALKAPRDGVVAEVRVVAGDFVEADAVLALLED"
     gene            3348..3983
                     /locus_tag="PK818_06970"
     CDS             3348..3983
                     /locus_tag="PK818_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82510.1"
                     /translation="MARLPLLAMLALLLGVAGCSTTVFESLPAGTATDCDPAWPGRWQ
                     PEGAIGEALPAREAVEIAADCRSATSKGERKPLHLTLVATRDGQYLQLHDDSGKPDCI
                     GKDKAHCGHVLFRYERSGDTIRLYDVDHARVAADIGSKKIRGYSERQDGGEVKTDKPV
                     HHNFIAGDGEHIARLLHRRPQVFASEPLLVLQRVPAAPAAGEAPAPPPGGQ"
     gene            3991..4881
                     /locus_tag="PK818_06975"
     CDS             3991..4881
                     /locus_tag="PK818_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HQY82511.1"
                     /translation="MPTEVRIVEVGARDGLQNEKVEIGTADKIALIDRLSATGLRSIE
                     ATAFVSPKWVPQMADAAEVLAGITRKPGVAYPVLVPNLQGYERARAAGATEVAVFTAA
                     SEAFNLKNTNATVEQSLERFRPVLERAHEDGVRVRGYVSTVLGCPYQGVVALADVVRV
                     SRTLHAMGCYEISLGDTIGVGTPAKARAMLAAVATEVPMPALAVHFHDTYGQALANVL
                     ACLEEGVAVVDAAVSGAGGCPYAKGASGNVASEDVVYMLHGLGIRTGIDLDALAATGR
                     WLAALLGRETGSKAGKALAA"
     gene            4878..7442
                     /locus_tag="PK818_06980"
     CDS             4878..7442
                     /locus_tag="PK818_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQY82512.1"
                     /translation="MKPASGVRSTMQLDAALVDLLAQLVEVGGKGGLSGECTQLMKDV
                     ATMLVETRAALKLEYSRYRALFDAVPDPVSIIAYDGTVLDLNKAGIDAYERPREDIIG
                     KPIEVLNPDLPRNHLAPVLEALRRGETHVIEVTNMRADGTRFPVEVHSAALEYDGRQA
                     MVAVARDLSARYEAEARYHSLVEGIDKAITLQDRNGRLHYINAAGMRMFGLRDDENLQ
                     REPDWDDWLVVDENGRPLPREQHPSARAIRLGTAVSSQVIGLYRRSTQRLAWLSVTSV
                     PQFAPGAKRPHMVLSLSSDITELKRDNALFERVQELARIGGWQWDRSRDALYLTAEAR
                     RILGTLRASCTLEDVLGGLHPPDREQLARTIAGVTEGTGFELELHGQHADGAPFWVRM
                     IGESESGGPGADLLTGTLQDITERKQAEESLRQQAQTDALTGLMNRDAILRQLDLRLA
                     HPASNGVGLLYVDLDRFKIVNDVLGHSAGDTLLIAAARRMRDAVASEGLISRFGGDEF
                     VVVCATGDDPGRPERLARRVQHAFTEAFRLDRDEFSVTTSIGIARAPADGETAQQLIQ
                     SADVAMYDSKRRRRNGWQSFSPELASRQQEKLQAETQLRRALDNREFHLVYQPQVDLR
                     TGRILYAEALVRWRNPQLGEMRPDRFIDLAETTGDIIRIGNWVLHEACAQMRRWREQD
                     LPIQRVAVNVSYRQFTGDNLGESVGKALADAGLPGHALELELTERVLVDDTPDTLLVF
                     DQLRALGVQLSIDDFGEGYSALNYLRRLPIHALKLSQSFLKGVPGKPSDVAICQAVAG
                     IAQSLGLGIVAEGVESEAQRDFLLRLGVNVGQGYLFAPGLLPGEFAKRVTAQQDTA"
     gene            7439..7933
                     /locus_tag="PK818_06985"
     CDS             7439..7933
                     /locus_tag="PK818_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY82513.1"
                     /translation="MSARGEITIRPIQPADDAAIAAIIRAVMPEFGAVGSGFAISDPE
                     VDWMSRAYAAPRHAYFVVERDGVVRGGGGIAPLIGGDAGTCELRKMYFMPDVRGIGAG
                     ALLMSRCLDAARDAGFAQCYLETLTGMDAAQRLYERSGFRRIGGPMGATGHGGCNTFY
                     LLEL"
     gene            8026..>8408
                     /locus_tag="PK818_06990"
     CDS             8026..>8408
                     /locus_tag="PK818_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY82514.1"
                     /translation="MQLASVRAVVTGGVSGLGLAVGRHLVANGGKVALFDVNDDKGAA
                     AIAELGDGNARYFRTDVTDEAGVAANVAAARDFLGGLNVAVNCAGILGSGRMIGRDGA
                     MKLSQFQSTVMVNLVGSFNVAKACA"
BASE COUNT         1308 a         2960 c         2962 g         1178 t
ORIGIN      
        1 aggctcgcgg cgttgaggct gtaggcaccc ggcaggtcga gcaccgcgta ctggcgtccg
       61 gacggcgcat gcaggcggcc ttccttgcgc tcgacggtga cgccagcgta gttcgcaacc
      121 ttctgccggc tgccggtcaa caggttgaac aacgcggtct tgccgcagtt cgggttgccg
      181 accagcgcga ttcgcggata tcgggcctgg aggttggcga cctgcggatt catgcgcccg
      241 ccccgcccgc cctgaggcgc acccgcgccg cttcgacctg gcgcagggcg aaacgggtga
      301 aaccgacctg gaccagcaac ggttccgccc cgaacgggcc ggcggcgacc acctccacct
      361 gctcgccggc aacgaagccg agttcgcgca ggcggcgggc gatggcatcg ttcggtgcca
      421 ggtcctcgac ggcttcgaca atggccggcg tgcggcgggc gagttggttc agcttcacga
      481 tgaaagccaa atgggaattg ttcgcaattc tagcaccgca gcggccatgc gggtgcgacc
      541 gctatcatgt gtggtcttgc acggaggtcc gatgagcgaa cgcctgcacc tgcaccgcga
      601 cggccccgtc gcccgcctgc gcatgacccg cgcgcaggtg cacaacgcct tcgacgcggg
      661 gctgatcgcc ggcatgaccg ccgcgctcga ggacgtggcg ggcgacgccg gcatccgcgt
      721 gctggtgctg gaagccgagg gcgcctcgtt ctccgccggc gccgacctca actggatgcg
      781 cggcatggcc gccgccagcg aagccgacaa ccgcgaggac tcgctcgccc tcgcccgcct
      841 gatgcgcacg ctggacgaac tgccgaagcc gaccatcgcc cgcgtgcagg gtgcggcgtt
      901 cggcggcggg gtgggcctgg tggcctgctg cgacatcgcg atcggcgtgc cggaggcgaa
      961 gttcggcctg accgaaagca ggctgggcct gctgccggcg gtgatctcgc cgtacgtgat
     1021 caacgcgatc gggccgcgcc acgcacgccg gtatttcgcc agcgcggaga tcttcgatgc
     1081 cgccgaagcg ctgcgcatcg gcctgctgca ccaggtggtg cccgcggacg aactggatgc
     1141 cgccgttcgc cagcagcttg cgctgttgct gaaggccggg ccgatcgcca gcgccagcgc
     1201 caagtcgctg gtgcgcgccg cctgcgcgca tgccgacggc gcccgccacg acgccgacaa
     1261 cgccgccctg atcgcgcgcc tgcgcgtgtc cccggaaggc caggaaggcc tgtccgcctt
     1321 cctcgacaag cgcaagccgc actggatcga cgcctgatgt tctcgaaaat cctgatcgcc
     1381 aaccgcggcg agatcgcctg ccgggtcatc cgcaccgcgc gcaggctcgg catccgcacc
     1441 gtcgccgtgt tctccgatgc cgatgccgac gcccagcacg tgcgcctcgc cgacgaggcg
     1501 caccacatcg gcggcccgcg cccgcaggac agctacctgc gcggcgacgc gatcatcgcg
     1561 gtggcgcagc gcaccggtgc gcaggcgatc catcccggct acggcttcct gtccgagaac
     1621 gccggcttcg ccgacgccgt tgcagcggcg gggatcgcct tcatcggccc gtccggcgcg
     1681 tcgatgcgca agatgggcag caaggccggc gccaaggagc tgatgcatgc cgccggcgtg
     1741 ccggtggtgc ccggctacac cggcgaggac cagtcgcccg acctgctgca gcgcgaggcc
     1801 gacgcgatcg gctatccgct gatgatcaag gccgcgcacg gcggcggcgg caagggcatg
     1861 cgcatcgtgc gtgattcggc cgaattcgca ccgatgctgg aaagctgcca gcgcgaggcg
     1921 gccaatgcgt tcggccgcga ccgggtgttg ctggagcgct acgtcgaaaa gccgcgccat
     1981 gtcgagatcc aggtgttcgg cgacggcttc ggcaacgcga tccacctcaa cgaacgcgag
     2041 tgctcggcgc aacgccgcta ccagaaggtg ctggaggaat cgccgtcgcc gttcctgacc
     2101 ggcgacctgc gcgaggcgat gggcgcggcc gcggtgctgg cggcgcgcgc gatcgattac
     2161 gtcaacgccg gcacggtgga gttcatcgtc ggccaggacg gcggcttcta cttcatggag
     2221 atcaacaccc ggctgcaggt cgagcatccg gtgaccgagg aaaccaccgg cctggacctg
     2281 gtcgaatggc agctgcgggt ggccgcgggc gagccgttgc cgctggcgca ggcggacgtc
     2341 ccgcggcacg gccacgcgat cgaggtacgg ctgtacgcgg aagacccgga cgccggcttc
     2401 ctgcccggct ccggcaccct gcaggcgttg cgcctgccgg caccgtcgcg ccacgtgcgg
     2461 atcgactccg gcgtgatcga gggcgatacc gtcagcattt tctacgaccc gatgatcgcc
     2521 aagctgatcg tctgggacgc ggaccgcccg cgtgcgctcg cgcgactgcg cgaggcactg
     2581 gcgggctgcg agatcgccgg cccgaagtcg aacatcggct tcctggagcg gctggtgcgg
     2641 cacccggccg tcgtcgatgc cagcatcgac accggctacc tcgaccgcca cctcgatgaa
     2701 ttcctcgccc ccgccggcac cgacatcacc ctgctgctgg cagcggtcag cgtccgcctg
     2761 ctcgcacagg aagcggatgc gtgcgccgcc gccggccacg acccgggttc gccatgggcg
     2821 atcgccgacg gctggcgact gggccatgcc ggcgcgcgcc ggctggcgtt cgccgacggc
     2881 gatcaccgga tcgatgtcat cgcccacggc catggcggca gctatcgcat cgaactcgac
     2941 ggcgcctgcc acgaggtcgc cgccgcgcgc ctgctcgacg gcacgctcag cgcccgcatc
     3001 gacggcgcga tgcgccgggt cggcgccctg gccagcgcgg agcgggtgct ggtgcacgat
     3061 ggcgagcgcc ggctgcagct ggcgtcggtg gaggtgtatc gtcgcggggc cgaagccggc
     3121 gccggtggcg aacaccggat ccgcgcgccg atgccgggac gggtcgtggt ggtgcagaag
     3181 ctgccgggcg atgccgttgg cgtgggggac gtggtgctgg tgatcgaagc gatgaagatg
     3241 gaactcgccc tgaaggcgcc gcgcgacggc gttgtcgccg aagtgcgggt cgtcgctggc
     3301 gatttcgtcg aggccgatgc cgtgctggcg ctgctggagg actgacgatg gcgcgcctgc
     3361 ccctgctggc gatgctcgcc ctgctgctgg gcgtggccgg ctgctcgacc accgtgttcg
     3421 aatccctgcc cgccggcacc gccaccgact gcgatccggc ctggccggga cgctggcaac
     3481 cggaaggtgc gatcggcgag gcactgccgg cacgcgaagc ggtggagatc gccgccgact
     3541 gccgcagcgc caccagcaag ggtgaacgca agccgttgca cctgaccctg gtcgccaccc
     3601 gcgacggcca gtacctgcag ctgcacgacg attccggcaa gccggactgc atcggcaagg
     3661 acaaggccca ttgcggccac gtgctgttcc gctacgagcg cagcggcgac accatccgcc
     3721 tgtacgacgt cgaccacgcc cgggtcgccg ccgacatcgg cagcaagaag atccgcggct
     3781 acagcgagcg ccaggacggc ggcgaggtca agaccgacaa gccggtccac cacaatttca
     3841 tcgccggcga cggcgagcac atcgcccgcc tgctgcaccg ccgcccgcag gtgttcgcca
     3901 gcgaacccct gctggtactg cagcgcgtgc ccgcagcgcc cgcagccggc gaggcccccg
     3961 cgccgccgcc gggaggccag tgagatgggc ctgcccacgg aggtccggat cgtcgaggtc
     4021 ggcgcgcgcg acggcctgca gaacgagaag gtcgagatcg gcaccgccga caagatcgcg
     4081 ctgatcgacc ggctgtcggc gaccggcctg cgcagcatcg aagccaccgc cttcgtcagc
     4141 ccgaaatggg tgccgcaaat ggcggatgcg gccgaggtgc tggccggcat cacccgcaag
     4201 ccgggggtcg cctacccggt gctggtcccg aacctgcagg gctacgaacg cgcgcgtgcc
     4261 gccggcgcga ccgaagtggc ggtattcacc gcagcctccg aagcgttcaa cctgaagaac
     4321 accaacgcca ccgtggagca gtccctggaa cgcttccggc cagtgctgga gcgcgcccac
     4381 gaggacggtg ttcgggtgcg cggctatgtc tccaccgtgc tcggctgccc gtaccagggc
     4441 gtggtggcgc tggccgacgt ggtgcgggtc tcgcgtacgc tgcacgcgat gggctgctac
     4501 gaaatctcgc tcggcgacac catcggcgtc ggcacgccgg caaaggcgcg cgcgatgctg
     4561 gcggcggtgg ccaccgaagt cccgatgccg gcgctggcgg tccatttcca cgacacctac
     4621 ggacaggcgc tggcgaacgt gctggcctgc ctggaggaag gcgtggcggt ggtggatgcg
     4681 gcggtgtccg gcgccggcgg ctgcccgtat gcgaagggtg ccagcggcaa cgtggccagc
     4741 gaggacgtgg tctacatgct gcatggcctg ggcatccgca ccggcatcga cctcgacgcg
     4801 ctggccgcga ccggccgctg gctggccgcc ctgctgggcc gcgagaccgg cagcaaggcc
     4861 ggcaaggcgc tcgccgcgtg aagcccgcat ccggcgtgcg ctccaccatg cagctcgacg
     4921 cggcgctggt cgacctgctg gcgcagctgg tcgaggtcgg cggcaagggc gggctgtccg
     4981 gcgaatgcac gcagctgatg aaggacgtcg cgaccatgct ggtcgagacc cgcgccgccc
     5041 tgaagctcga atacagccgc taccgcgccc tgttcgacgc cgtgccggac ccggtcagca
     5101 tcatcgccta cgacggcacc gtgctcgacc tcaacaaggc cggcatcgat gcctacgagc
     5161 gcccgcgcga ggacatcatc ggcaagccga tcgaagtgct caaccccgac ctgccgcgca
     5221 accacctggc gccggtgctg gaggcgttgc gccgcggcga gacccacgtc atcgaagtca
     5281 ccaacatgcg cgccgatggc acccgcttcc cggtggaggt gcattcggcc gcgctggaat
     5341 acgacgggcg ccaggccatg gtcgcggtgg cgcgcgacct cagcgcccgc tacgaggcgg
     5401 aagcgcgcta ccacagcctg gtggaaggca tcgacaaggc gatcaccctg caggaccgca
     5461 acggcaggct gcactacatc aacgccgccg gcatgcgcat gttcggcctg cgcgacgacg
     5521 aaaacctgca acgcgagccg gactgggatg actggctggt ggtcgacgaa aacgggcgcc
     5581 cgttgccgcg cgagcagcat ccctcggcgc gggcgatcag gctcggcacc gcggtcagca
     5641 gccaggtgat cgggctttac cggcgcagca cccagcgcct ggcctggctg tcggtgactt
     5701 cggtgccgca gttcgcgccc ggcgcgaagc gcccgcacat ggtgctgagc ctgagcagcg
     5761 acatcaccga gctcaagcgc gacaacgcgc tgttcgaacg cgtgcaggag ctcgcgcgga
     5821 tcggcggctg gcaatgggac cgcagccgcg acgccctgta cctgaccgcg gaggcgcgac
     5881 gcatccttgg caccctgcgc gcatcgtgca cgctcgagga tgtgctgggc ggcctgcatc
     5941 cgccggatcg cgaacagttg gcgcggacga ttgccggcgt caccgagggc accggcttcg
     6001 aactggagct gcatggccag catgccgacg gcgccccgtt ctgggtgcgc atgatcggcg
     6061 aatcggaatc cggcggcccc ggcgccgacc tgctgaccgg caccctgcag gacatcaccg
     6121 agcgcaagca ggcggaggag tcgctgcgcc agcaggcgca gaccgacgcc ctgaccggcc
     6181 tgatgaaccg cgacgcgatc ctgcgccagc tggaccttcg cctcgcgcat ccggccagca
     6241 acggcgtcgg gctgctgtac gtggacctcg accgcttcaa gatcgtcaac gacgtgcttg
     6301 gccacagtgc aggcgatacc ttgctgatcg ccgccgcgcg gcgcatgcgc gacgcagtcg
     6361 ccagcgaagg cctgatctcg cgcttcggcg gcgacgaatt cgtggtggtc tgcgccaccg
     6421 gcgatgaccc cggccgcccg gaacggctgg cgcgccgggt gcagcacgca ttcaccgagg
     6481 cgttccggct cgatcgcgac gaattcagcg tcaccaccag catcggcatc gcgcgcgcgc
     6541 cggccgatgg cgagaccgcg cagcagttga tccagagcgc ggacgtggcg atgtacgaca
     6601 gcaagcggcg gcggcgcaac ggctggcagt cgttctcgcc ggaactcgcc tcgcgccagc
     6661 aggaaaagct gcaggcggag acccaactgc ggcgtgcgct cgacaaccgc gagttccacc
     6721 tggtctacca gccgcaggtg gacctgcgca cgggccgcat cctgtatgcc gaggcgctgg
     6781 tgcgctggcg caacccgcaa ctgggcgaga tgcggccgga ccgcttcatc gacctggcgg
     6841 agaccaccgg cgacatcatc cgcatcggca actgggtgct gcacgaagcc tgcgcgcaga
     6901 tgcgccgctg gcgcgagcag gacctgccga tccagcgggt cgcggtcaac gtctcctatc
     6961 gccagttcac cggcgacaac ctgggcgaga gcgtgggcaa ggcactggcc gatgccggcc
     7021 tgcccggcca tgcgctcgag ctcgagctca ccgagcgggt gctggtcgac gacaccccgg
     7081 acaccctgct ggtgttcgac cagttgcggg cgctgggcgt gcaattgtcg atcgacgatt
     7141 tcggcgaggg ctacagcgcg ctcaattacc tgcgcaggct cccgatccat gcgctgaagc
     7201 tgagccagtc gttcctgaag ggcgtgccgg gcaagccctc cgacgtggcg atctgccagg
     7261 ccgtcgccgg catcgcccag agcctgggcc tgggcatcgt cgccgagggc gtggaaagcg
     7321 aagcccagcg cgatttcctg ctgcggctcg gggtcaacgt gggccagggc tacctgttcg
     7381 cgccggggct gctgccgggc gagttcgcca agcgcgtgac cgcgcagcag gacaccgcat
     7441 gagcgcacgc ggcgaaatca cgatccgacc gatccagccc gcggacgacg ccgcgatcgc
     7501 cgcgatcatc cgcgcggtga tgcccgagtt cggcgcggtc ggcagcggat tcgcgatcag
     7561 cgacccggaa gtggactgga tgtcgcgcgc ctatgccgcg ccgcggcatg cgtatttcgt
     7621 ggtcgaacgc gacggcgtgg tgcgcggtgg cggcggcatc gcgccgctga tcggcggcga
     7681 tgccggcacc tgcgagctgc gcaagatgta tttcatgccg gacgtgcgcg ggatcggtgc
     7741 cggtgccttg ctgatgtcgc gttgccttga cgccgcccgc gacgcgggct tcgcccagtg
     7801 ctacctggaa accctgaccg gcatggatgc ggcgcaacgc ctgtacgaac gcagcgggtt
     7861 ccggcgcatc ggcggaccga tgggcgccac cgggcatggg ggctgcaata cgttctacct
     7921 gctggagctc tagcggactt ctcgcggaac cgcaccggcg gccgacgtgc ccggcatgcc
     7981 atcaccatcc ggtaaaatcg cggttttccg cccgcaggaa tccccatgca actcgcatct
     8041 gtccgcgccg tggtcaccgg cggcgtctcc ggccttggcc tggccgtggg ccggcacctg
     8101 gtcgccaatg gcggcaaggt cgccctgttc gacgtcaacg acgacaaggg cgccgccgcc
     8161 atcgccgaac tcggcgacgg caacgcccgc tacttccgca ccgacgtcac cgacgaagca
     8221 ggagtggcgg ccaatgtcgc cgccgccagg gacttcctcg gcggcctcaa cgtggcggtc
     8281 aattgcgccg gcatccttgg ctccggccgg atgatcgggc gcgacggggc gatgaagctt
     8341 tcccagttcc agtccacggt gatggtcaac ctggtcggca gcttcaacgt cgccaaggcc
     8401 tgcgccga
//
LOCUS       DAORUK010000087         8272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_9566_length_8272_cov_18.713642, whole genome shotgun sequence.
ACCESSION   DAORUK010000087 DAORUK010000000
VERSION     DAORUK010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9566_length_8272_cov_18.713642"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PK818_06995"
     CDS             complement(<1..649)
                     /locus_tag="PK818_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase"
                     /protein_id="HQY82515.1"
                     /translation="MARLDQSPKTVSIVDLSLDGRGVARWPEGHANAGKAVFVSGALP
                     GETVSVQQTARSRHFDEAKTLEVIEASPDRVEPRCPHFGTCGGCVLQHFAEERQIESK
                     QRVLLENLQRIGHVAPQSVLPPLRGDSWGYRRKGRFSVRRVEKKDKTLVGFREQDPRF
                     VADIGECHVVVPQLGFKVEALGALVDGLDARRDIPQIEFIAGDAQIALVFRHLQPL"
     gene            complement(737..1360)
                     /locus_tag="PK818_07000"
     CDS             complement(737..1360)
                     /locus_tag="PK818_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HQY82516.1"
                     /translation="MKQVFGIPVVGALLLTACVTINVYFPAAEAKEAAREFVEKVIDE
                     ADKVNMQDESRKDSSKDGGGGMAMLQRRFDFDPWMLIGIGSAHAQSAPDISIKTPAIQ
                     AIQARMEARFNSTLRAGFDSGALGFTSDGLISVRDAGKLELKDRVAMNAAVADDNRDR
                     KAVYREVAVANGHPEWEGQIRDVFAKQWVDSARSGWWYQSGGGWKQK"
     gene            complement(1408..>2207)
                     /locus_tag="PK818_07005"
     CDS             complement(1408..>2207)
                     /locus_tag="PK818_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82517.1"
                     /translation="REDAVVPLLGGRMTFRDLTIRPPQGEAGADIRFGLVLDDIDFGR
                     VSNALGLPAFQGRLSGTIPNARYANERIDFDGGLAMQLFDGQVAFSSLALERPFGSAP
                     SLSADIALDDLDLLPLTEVLGFGSITGRIDGRISGLRLVDWTPVAFDARFITDARPGV
                     AQRISQRAVQEISSVGDASFVSSLQGQLIGLFDDFGYRRIGIGCRLVNEVCAMSGLHS
                     ANNAFTIVEGAGLPRLGVVGFNRQVDWPTLVERLTAVGTGEVKPVFE"
     assembly_gap    2208..2307
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2308..3584)
                     /locus_tag="PK818_07010"
     CDS             complement(<2308..3584)
                     /locus_tag="PK818_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82518.1"
                     /translation="MPRPIIAATLALLAALSAAAADARGMQARIDRVVTPVATLEQVR
                     VRVDWLAGASTGELQLWAGRVDAPDLGYRYRDLHWRCPLQRDGEDGWRCEGALRSGRS
                     APLQLAVRLDAATTHASLGQGKARIALDRDAASPDLTTIDLTRVPAQWAQALLAKAWP
                     EARFTSGELDARLDIDAAAGRPLRISGPLALRGIGLETADASIAGEKLGGRFAIDYRT
                     TPALSLLAVDGELRGGEFLAGNTYVALPATPVGLRIDALQHAGAGWELPRIEWRDGDA
                     LAAVGSARFATDASLQALDLGVHSGDMAPLRQRYLSGWLGLFGMGDVDLHGAMDLRVQ
                     VRDGLLQVADASLDGVDLRDPDERFVFDGLQGDVRYSAGAPVQSELRWNSGKLYGLEF
                     DAARLPFASADGLLRFREDAVVPLLGGRMTFRDL"
     gene            3618..4328
                     /locus_tag="PK818_07015"
     CDS             3618..4328
                     /locus_tag="PK818_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY82519.1"
                     /translation="MNPSPTALPRLLLVEDDPVSAAFLREAAAALPALVDVAGTVAEA
                     LSAASIQCHDLYLIDANLPDGRGETLLQDLRGRAPAAPALAHTAARDAPMRERLLAAG
                     FVEVLCKPLAVTELHAALRRHLELPPPACGKQPNWDDAAALRAVGGQQAHVEALRGLF
                     LRELPGQRQRIEAAAACGDEEAIRAELHRLAASCGFVGAARLADAVRELQAAPRDARA
                     LLRFGLTASDLPGEKGPE"
     gene            complement(4378..5139)
                     /gene="recO"
                     /locus_tag="PK818_07020"
     CDS             complement(4378..5139)
                     /gene="recO"
                     /locus_tag="PK818_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HQY82520.1"
                     /translation="MRYTAEPAFVLHARPWRETSLLVEVLSAEHGRVGLVARGVQGAK
                     RHVLRAALQPLQSIRFDAVQRGELAQLATAEATDAAPLLQGDAVMAAFYINELCLRLA
                     PRQDPQPDLHAAYGLARERLREGEALAWTLRRFERDLLEALGFGFAFDIDGDGRAVDP
                     AARYTLDPEHGPRRVLGERNDARRESAMGEALLALAADAMPQRDVLASLRLPLRTILL
                     HTLGGRGLKSWEMLGALGRLGRVGPAAERGEKGPE"
     gene            complement(5262..6182)
                     /gene="era"
                     /locus_tag="PK818_07025"
     CDS             complement(5262..6182)
                     /gene="era"
                     /locus_tag="PK818_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014504020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HQY82521.1"
                     /translation="MSNEKQRSGAVAVIGRPNVGKSTLVNALVGAKISITSNRPQTTR
                     HRLLGIATFPGGQLQLVDTPGIHGEQGKRSGKAMNRMMNRAARGALEGVDAAVLVVEA
                     GRWDAEDGLAYDALHAAGMPVVLVVNQVDKVKDKGALLPFIAKVSEGRDFAAVHPLSA
                     LKRKGLESLVETLLALVPEAPPMYAEDEITDKSQRFLAGELVREQLMRQLGEELPYAT
                     TVEVEKFEEDLTGRQPIYRIDAVVWVERDGQKAIVIGKGGERLREIGSRARQQMEHLF
                     GSKVFLQTWVRVREGWSDDEAALRSLGYHD"
     gene            complement(6179..6856)
                     /gene="rnc"
                     /locus_tag="PK818_07030"
     CDS             complement(6179..6856)
                     /gene="rnc"
                     /locus_tag="PK818_07030"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HQY82522.1"
                     /translation="MAPRPDRIDTFAGHDFTDPGLLRQALTHRSAGSPHNERLEFLGD
                     ALVNQFVAEALYRYWPKADEGALTRARAELVRESALAAIARGLDLGDSLVLGPGELKT
                     GGHRRDSILADALEALVAAIHLDAGFEACRAAVLPWFEPLIAALPPPNKVGKDAKTRL
                     QEWVQARGKPLPQYALLEEGGDDHARTFRVGCTLREPSVHSEGEGSSRRAAEQQAADA
                     ALRIIEA"
     gene            complement(6840..7220)
                     /locus_tag="PK818_07035"
     CDS             complement(6840..7220)
                     /locus_tag="PK818_07035"
                     /inference="COORDINATES: protein motif:HMM:NF027463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HQY82523.1"
                     /translation="MKNRKQSGMTMLGFLITLSVVMFFIYCGMKIVPMYIEFYSVEKA
                     LASIAKEEGAATAPKDKIRAMFTRHMTIDYVSIVKPEMLKIESTDAGYNLTVAYERRE
                     VLFANLDVVGKFNAQQEVRRGAAP"
     gene            complement(7236..8039)
                     /gene="lepB"
                     /locus_tag="PK818_07040"
     CDS             complement(7236..8039)
                     /gene="lepB"
                     /locus_tag="PK818_07040"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQY82524.1"
                     /translation="MVWFETILVSLTALTGLVWLLDKLFLAKRRAAAAGVLEEAKEPV
                     VVDYAKAFFPILALVLGLRSFVAEPFRIPSSSMMPTLLIGDFILVNKFAYGLRWPITN
                     KKFLDNGEPQRGDVAVFRPPHHPREDWIKRVVGLPGDTIGYHDNKVTVNGRELPYRAE
                     GLYEGVGQGARETGASKLTEGLPGRPHFVLERQDLPFIPQGQGEGDWKVPAGHYFVMG
                     DNRDNSEDSRFWGVLPEANLRGKAFLVWMNFDWSSARKVDASRIGTKIP"
     gene            complement(8071..>8272)
                     /locus_tag="PK818_07045"
     CDS             complement(8071..>8272)
                     /locus_tag="PK818_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HQY82525.1"
                     /translation="IGAQIIARTTVKAMRKNVLAKCYGGDATRKKKLLEKQKAGKKRM
                     KQVGSVEIPQEAFLAVLQMDNK"
BASE COUNT         1223 a         2915 c         2713 g         1321 t
ORIGIN      
        1 agagtggctg caggtggcgg aacacaagcg cgatttgtgc atcgccggcg atgaattcga
       61 tctgcgggat gtcgcggcgc gcgtccaggc catcgaccag tgcacctagc gcctcgacct
      121 tgaagcccag ttgcgggacc acgacatggc attcgccgat gtcggccacg aagcgcgggt
      181 cctgttcgcg gaacccgacc agggtcttgt ccttcttctc gacccggcgc acggagaagc
      241 ggcccttgcg gcgatagccc cagctgtctc cgcgcaacgg cggcagcacg ctttgtggcg
      301 cgacgtggcc gatgcgctgc aggttctcca gcaggacccg ctgcttggac tcgatctgcc
      361 gctcttccgc gaagtgctgc agcacgcagc cgccgcaggt gccgaaatgc ggacaacgcg
      421 gctcgacgcg atccggcgag gcctcgatga cctccagcgt cttcgcttcg tcgaagtgtc
      481 gcgagcgggc ggtctgctgc acgctcaccg tttcacccgg cagcgcgccg gagacgaaca
      541 cggccttgcc ggcattcgca tgaccttcgg gccaacgcgc gacgccgcgg ccatccaggc
      601 tgagatcgac gatggaaacg gtcttggggg attggtctag gcgggccacg gaatcaggcg
      661 atggggatgg cccgccattg tcgcaccctg caaaagcaaa cggcccaccg aagcgggccg
      721 ttgccgggaa acaggcttac ttctgcttcc agccgccgcc gctctggtac caccagccac
      781 tgcgggcgct gtccacccat tgcttcgcga atacgtcgcg gatctggccc tcccattccg
      841 gatggccgtt ggccaccgcg acctcgcgat agaccgcctt gcggtcgcgg ttgtcgtcgg
      901 ccaccgcggc gttcatcgcc acccggtcct tcagctccag cttgccggcg tcgcgcacgc
      961 tgatcaggcc gtcgctggtg aagccgagcg cgccgctgtc gaagcccgcg cgcagggtgc
     1021 tgttgaagcg cgcttccatg cgcgcctgga tggcctggat cgccggcgtc ttgatgctga
     1081 tgtcgggcgc cgactgcgcg tgcgcgctgc cgatgccgat cagcatccac gggtcgaaat
     1141 cgaagcggcg ctgcagcatg gccatgccgc caccgccgtc cttcgaggaa tccttgcggc
     1201 tctcatcctg catgttgacc ttgtcggcct cgtcgatcac cttctcgacg aactcgcgcg
     1261 ccgcttcctt ggcctcggcc gccgggaagt acacattgat ggtcacgcag gcggtgagca
     1321 gcaacgcccc cacgaccggt atgccgaaaa cctgtttcat tgcattgctc ctgttggatc
     1381 acgccaaagt ggatctggca tcgcggatca ttcgaacacc ggcttcactt cgcccgtccc
     1441 gaccgccgtc aaccgctcca ccagggtggg ccaatccacc tgccggttga agccgaccac
     1501 gcccaggcgc gggagtccag cgccttccac gatagtaaag gcgttgttgg ctgaatgcag
     1561 gccggacatc gcgcagacct cgttcaccag ccggcagccg atgccgatcc tgcggtagcc
     1621 gaaatcgtcg aacaggccga tcagctggcc ttgcaggctg ctcacgaagc tggcgtcacc
     1681 gacgctgctg atctcctgca ccgcgcgctg gctgatccgc tgcgccacgc cggggcgggc
     1741 atcggtgatg aagcgcgcgt cgaacgcgac cggcgtccag tccaccaggc gcaggccgct
     1801 gatgcggccg tcgatgcggc cggtgatgct gccgaagccg agcacttcgg tcagcggcag
     1861 caggtcgagg tcatccagcg cgatgtccgc gctcaggctg ggcgcgctac cgaacgggcg
     1921 ttccagcgcc agcgagctga aagcgacctg gccatcgaac aactgcatgg ccaggccgcc
     1981 gtcgaaatcg atgcgttcgt tggcatagcg tgcgttcggg atggtgccgc tcaagcggcc
     2041 ctggaacgcc ggcaggccca gcgcgttgga gacgcggccg aaatcgatgt cgtccagcac
     2101 cagaccgaag cggatgtcgg cgcccgcctc accctgtggc gggcggatgg tcaggtcgcg
     2161 gaaggtcatg cgtccgccga gcagcggcac cacggcgtcc tcgcggannn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnagg tcgcggaagg tcatgcgtcc gccgagcagc
     2341 ggcaccacgg cgtcctcgcg gaagcgcagc aggccgtcgg cgctcgcgaa cggcagacgc
     2401 gcggcatcga actccaggcc gtacagcttg ccgctgttcc agcgcagctc gctttgcacc
     2461 ggcgcgccgg ccgaatagcg cacgtcgccc tgcaggccat cgaacacgaa gcgttcgtcc
     2521 ggatcgcgca ggtccacgcc atccagcgac gcatcggcga cctgcagcaa cccgtcgcgc
     2581 acctgcacgc gcaggtccat cgcaccatgc aggtcgacgt cgcccatccc gaacaggccc
     2641 agccagccgg acaggtagcg ttgccgcagc ggcgccatgt cgccgctgtg cacgccgagg
     2701 tcgagcgcct gcaggctggc atcggtggcg aagcgggcac tgcccacggc ggcgagcgcg
     2761 tcgccgtcgc gccattcgat gcgcggcaat tcccagccgg cgcctgcgtg ctgcagggca
     2821 tcgatgcgca accccaccgg cgtggccggc aacgcgacat aggtgttgcc ggccaggaac
     2881 tcgccgccgc gcagttcgcc atccaccgcc agcaacgaca gcgccggcgt ggtgcggtag
     2941 tcgatcgcga aacggccgcc gagcttttcg ccggcgatgg aagcgtcggc ggtttccagc
     3001 ccgatcccgc gcaacgcgag cggacccgaa atgcgcagcg ggcggccggc agcagcgtcg
     3061 atgtccagcc gcgcatccag ctcgcctgac gtgaagcgcg cctccggcca cgccttggcc
     3121 agcagcgcct gcgcccattg cgcgggcacg cgggtgaggt cgatcgttgt caggtccggc
     3181 gaagcggcat cgcgatccag cgcgatccgc gccttgccct gccccagcga ggcatgggta
     3241 gtcgcggcat caagacgcac cgccagttgc aggggtgccg agcgtccgga gcgcaacgcg
     3301 ccctcgcaac gccagccgtc ctcgccgtcg cgttgcagcg ggcagcgcca gtggaggtcg
     3361 cgatagcggt agccgaggtc gggcgcatcg acgcgaccgg cccacagttg cagctcgccg
     3421 gtggaggcac ccgccagcca gtccacccgc acccgcacct gctcgagggt cgccaccggg
     3481 gtcaccaccc ggtcgatccg cgcctgcatg ccgcgcgcat ccgccgccgc cgcgctcagc
     3541 gcggccagca gggccagggt tgcggctatg atcggtcgcg gcatcccggc agcatacaaa
     3601 ccccggatcg cgaaaccatg aatcccagcc cgactgcgtt gccgcgcctg ctgctggtcg
     3661 aagacgaccc ggtcagcgcg gcgttcctgc gcgaagccgc ggccgcgttg ccggcgctgg
     3721 tcgacgtcgc cggcacggtt gccgaggccc tgtccgcggc ctcgatccag tgccacgacc
     3781 tgtacctgat cgacgccaac ctgcctgacg ggcgcggcga gaccctgctg caggatttgc
     3841 gcgggcgtgc gcccgcggcg ccggcgctgg cgcacaccgc cgcgcgcgac gcccccatgc
     3901 gcgaacgcct gctggccgcg ggcttcgtcg aagtgctgtg caagccgctg gcggtgaccg
     3961 agctgcatgc ggcgctgcgc cgccacctcg agctgccgcc accggcctgc ggcaagcagc
     4021 cgaactggga cgacgccgcc gcgctgcgcg ccgtcggcgg ccagcaggcg catgtggagg
     4081 ccctgcgcgg cctgttcctc cgggaactgc ccggccagcg ccagcgcatc gaggccgcgg
     4141 cggcctgcgg cgacgaggag gcgatccggg cagaactgca tcggctggcc gccagttgcg
     4201 gcttcgtcgg tgcggcgagg ttggcggacg cggtgcgcga actgcaggcg gcgccgcggg
     4261 atgcgcgagc cttgctgcgg ttcggtctca ccgccagcga cttgcctgga gaaaaggggc
     4321 cagagtgaag tttcccgttc ggcatcctgg gcgtacggga ggcgctcgat gggaaattca
     4381 ctctggcccc ttttctcccc gttccgctgc cggccccact cggcccagtc gccccagtgc
     4441 gcccagcatc tcccaggact tcaagccgcg cccaccgagg gtgtgcagca ggatcgtgcg
     4501 cagcggcagg cgcaggctgg cgaggacgtc gcgttgcggc atggcgtctg ccgccagtgc
     4561 aagcagcgcc tcgcccatcg ccgattcgcg gcgtgcatcg ttgcgttcgc ccagcacccg
     4621 gcgcggcccg tgctccggat ccagcgtgta gcgcgcggcc ggatccaccg cgcgcccgtc
     4681 gccatcgatg tcgaaggcga agccgaagcc cagcgcctcc agcaggtcgc gctcgaagcg
     4741 gcgcagggtc caggccagcg cctcgccctc gcgcaggcgt tcgcgcgcca gcccgtaggc
     4801 ggcatgcagg tcgggctgcg ggtcctgccg cggggccagc cgcaggcaca gttcattgat
     4861 gtagaacgcg gccatcaccg catcgccctg cagcaacggg gcggcatcgg tcgcttcggc
     4921 ggtcgcgagt tgcgcgagtt cgccgcgttg cactgcatcg aagcggatcg actgcagcgg
     4981 ctgcagcgcg gcgcgcagca cgtggcgctt cgcgccctgc acgccacgcg ccaccaggcc
     5041 cacgcggcca tgttcggcgc tcagcacctc gaccagcagg ctggtctcgc gccatgggcg
     5101 cgcgtgcagg acgaaggcgg gttcggcggt gtagcgcatg ccctagcgcc tccacgtggc
     5161 cgaagcgcag ggggaggttg gaacggggtg cttttccatc gtagacaagt gctacccctc
     5221 cccggccctc ccctgcttcg caagggcggg aaaaagcctc gtcagtcgtg gtagcccagc
     5281 gagcgcagtg cggcttcgtc gtcgctccag ccctcgcgca cgcgcaccca ggtctgcagg
     5341 aacaccttgc tgccgaacag gtgctccatc tgctggcgcg cgcggctgcc gatctcgcgc
     5401 aggcgctcgc cgcccttgcc gatcacgatc gccttctggc cgtcgcgttc gacccagacc
     5461 accgcatcga tgcggtagat cggctggcgg ccggtcaggt cttcctcgaa tttctcgacc
     5521 tcgaccgtgg tcgcgtacgg cagttcctcg ccgagctggc gcatcagctg ctcgcgcacc
     5581 agttcgccgg ccaggaagcg ctggctcttg tcggtgatct cgtcctcggc atacatcggc
     5641 ggcgcttccg gcaccagcgc cagcagcgtt tcgaccagcg actccaggcc cttgcgcttg
     5701 agcgcagaca gcgggtgcac ggcggcgaaa tcgcggccct cgctgacctt ggcgatgaac
     5761 ggcagcagcg cccccttgtc cttgaccttg tcgacctggt tcaccaccag caccaccggc
     5821 atgccggccg cgtgcagggc gtcgtaggcg agtccgtcct cggcatccca gcgcccggcc
     5881 tcgaccacca gcaccgccgc atccacccct tccagggcgc cgcgggcggc acggttcatc
     5941 atccggttca tcgccttgcc cgagcgcttg ccctgttcgc cgtggatgcc gggcgtgtcc
     6001 accagctgca gctggccgcc ggggaaggtg gcgatgccga gcagccggtg gcgcgtggtc
     6061 tgcggccggt tggaggtgat gctgatcttg gccccgacca gcgcgttgac cagggtcgac
     6121 ttgccgacgt tcgggcggcc gatcaccgcc accgccccgc tacgctgctt ttcgttgctc
     6181 atgcttcgat gatccgtaac gcggcatccg ccgcctgttg ttcggcggca cggcgcgaac
     6241 tgccctcgcc ctcgctgtgc accgagggtt cacgcagcgt gcagcccacc cggaacgtgc
     6301 gtgcgtggtc gtcgccgccc tcttccagca gggcgtattg cggcagcggc ttgccgcgcg
     6361 cctgcaccca ttcctgcagc cgtgtcttcg cgtccttgcc gaccttgttc ggcggcggca
     6421 gcgcggcgat caagggctcg aaccacggca gcaccgccgc gcggcaggcc tcgaagccgg
     6481 cgtccaggtg gatcgcggcg accagcgcct ccagcgcgtc ggccaggatc gaatcgcggc
     6541 gatggccgcc ggtcttgagt tcgccggggc cgagcacgag gctgtcgcca aggtcgagcc
     6601 cgcgggcgat cgcggccagc gcggattccc tcaccagttc ggcgcgcgca cgcgtcagcg
     6661 cgccctcgtc ggccttcggc cagtaccggt aaagcgcctc ggcgacgaac tggttgacca
     6721 gcgcgtcgcc gaggaactcc aggcgttcgt tgtgcgggct gccggcgctg cgatgggtca
     6781 gcgcctgccg cagcaggccc gggtcggtga agtcatggcc ggcgaacgtg tcgatgcggt
     6841 cagggcgcgg cgccacgcct cacttcctgt tgcgcattga acttgccgac gacgtcgagg
     6901 ttggcgaaca ggacctcgcg tcgctcgtag gccaccgtga ggttgtagcc ggcatcggtg
     6961 gattcgatct tcagcatctc cggcttgacg atggagacgt agtcgatcgt catgtgccgg
     7021 gtgaacatcg cgcggatctt gtccttcggg gcggtggccg cgccttcctc cttggcgatg
     7081 ctggcgagcg ccttctcgac cgaatagaac tcgatgtaca tcggcacgat cttcatgccg
     7141 cagtaaatga agaacatgac caccgacagg gtgatcagga atccgagcat ggtcatgccg
     7201 ctttgcttgc gattcttcat gttccctctc cgtggttatg gaattttggt gccgatcctc
     7261 gaggcatcga ccttgcgcgc ggaactccag tcgaagttca tccagaccag gaacgccttg
     7321 ccgcgcaggt tcgcttccgg caacacgccc cagaaccggc tgtcctcgct gttgtcgcga
     7381 ttgtcgccca tgacgaaata atgccccgcc ggcaccttcc agtcaccttc gccctgtccc
     7441 tgcgggatga acggcagatc ctggcgctcg aggacaaagt gcggacgccc gggcaagccc
     7501 tcggtcagct tggatgctcc ggtctcgcgc gcgccctggc caacgccttc gtacaggccc
     7561 tcggcgcggt acggcagctc gcggccgttc accgtcacct tgttgtcgtg gtagccgatg
     7621 gtgtcccccg gcaacccgac cacccgcttg atccagtcct cgcgagggtg gtgcggcggc
     7681 cggaacacgg ccacgtcgcc gcgctgcggc tcgccgttgt ccaggaactt cttgttggtg
     7741 atcggccagc gcaggccgta ggcgaacttg ttgaccagga tgaaatcgcc gatcagcagg
     7801 gtcggcatca tcgagctgga cgggatccgg aacggctccg cgacgaagct gcgcaggccc
     7861 agcaccagcg ccaggatcgg gaagaaggcc ttggcgtagt cgaccaccac cggctccttc
     7921 gcctcctcca gcaccccggc ggcggcggcc cggcgcttgg ccaggaacag cttgtccagc
     7981 agccagacga ggccggtcag ggcggtcagc gagaccagga tcgtttcgaa ccacaccatc
     8041 atgccgcgtt ctccgggagt tgcatcgcgc tcacttgttg tccatctgca gcacggcgag
     8101 gaaggcttcc tgcgggattt ccacgcttcc cacctgcttc atccgcttct tgcccgcctt
     8161 ctgcttctcc agcagcttct tcttgcgcgt cgcatcgcca ccgtagcact tggccaacac
     8221 gttcttgcgc atcgccttga ccgtggtgcg ggcgatgatc tgcgcgccga tc
//
LOCUS       DAORUK010000088         8225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_9675_length_8225_cov_19.163647, whole genome shotgun sequence.
ACCESSION   DAORUK010000088 DAORUK010000000
VERSION     DAORUK010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9675_length_8225_cov_19.163647"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2018)
                     /gene="carB"
                     /locus_tag="PK818_07050"
     CDS             complement(<1..2018)
                     /gene="carB"
                     /locus_tag="PK818_07050"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002806440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HQY82526.1"
                     /translation="MPKRTDIKTILIIGAGPIVIGQACEFDYSGAQACKALRDEGYRV
                     VLVNSNPATIMTDPEMADAVYIEPINWQTVEKIIAKERPDALLPTMGGQTALNCALDL
                     ADHGVLEKYNVELIGAKRDAIRMAEDRELFRVAMAEIGLECPKAEVARSLEQALDIQT
                     RVGYPTIIRPSFTLGGSGGGIAYNREELIEIVNRGLELSPTSEVLIEESVLGWKEFEM
                     EVVRDTADNCIIVCSIENLDAMGVHTGDSITVAPAQTLTDKEYQRLRNASIAVLRKIG
                     VDTGGSNVQFGINASNGRVVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LKNEITGGKTPASFEPAIDYVVTKIPRFAFEKFPAADARLTTQMKSVGEVMAIGRTFQ
                     ESLHKALRGLETGKVGLDPTGLDLVDEGDLATLRRELKEPGPERIFHLADAFRAGMSV
                     EDVHALSFVDPWFLDQIEGLIDAEVDIAHRGLDGLDAKRLRALKRKGFSDTRIAQLTG
                     TNESAVRALRKAFGVRPVYKRVDSCAAEFATTTAYLYSTYEDECEAQPSDRQKVMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPLTLE
                     DVLEIVELEKPRGVIVQYGGQTPLKLAKALEANGVPIIGTSPDSIDLA"
     gene            complement(2020..3171)
                     /gene="carA"
                     /locus_tag="PK818_07055"
     CDS             complement(2020..3171)
                     /gene="carA"
                     /locus_tag="PK818_07055"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HQY82527.1"
                     /translation="MNIPAILALEDGTVFEGTSVGAAGLSVGEVVFNTAMTGYQEILT
                     DPSYARQLVTLTYPHIGNTGCTEQDDEAGQAWAAGLIVRDVPRRPSNWRSQVALPEWL
                     QQRGIVAIADIDTRKLTRILRDGGAQNGALLAGTTVSERLDADKAIEAARKFPGLKGM
                     DLAKVVSTPAAYPWHDGELDLDSGTFNRVDHRHKVVAYDFGAKHNILRMLAERGCDVT
                     VVPAQTPAADVLALQPDGVFLSNGPGDPAPCDYAIAAIREFIAAKIPTYGICLGHQLL
                     GLAAGAQTMKMPHGHHGANHPVIDIDSGRVMITSQNHGFCIDETTLPANARVTHRSLF
                     DGSNQGIELTDAPAFSFQGHPEASPGPHDVAPLFDKFVGLMANTHAGAR"
     gene            complement(3284..3994)
                     /locus_tag="PK818_07060"
     CDS             complement(3284..3994)
                     /locus_tag="PK818_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010367114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HQY82528.1"
                     /translation="MSEATIRLLVHGGNGRMGQALQRLGDATAGCQVVAAVSRQAGAR
                     VVEGIPQFAASELAGVPEFDVAIDFSLPQGFDAVLGLCLSRGKPLVSGTTGLSTQQLA
                     ALDAAAGTIAVAWASNFSLGVALLHELVERAARALPGWDCDILETHHVRKLDAPSGTA
                     LTLGAAAAQGGASPRYASIRAGDIVGEHLVQFATAGERIELVHRASNRDIFARGALHV
                     ARAIHGRAAGRHRIADLL"
     gene            complement(4053..6260)
                     /locus_tag="PK818_07065"
     CDS             complement(4053..6260)
                     /locus_tag="PK818_07065"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HQY82529.1"
                     /translation="MTFRNPTDFADAGRTDPGLLLDALQKQAIERVVPALDRALGRVD
                     DYLFDRSQSGNDNLGLTALRDLRRVRSQIAQRFEQALISGFRALRDPRAASAMLGEHS
                     GLSLLSEDALEEQLANEQLAESMSRMHAPSLELLGKRIAHVLGRDELKATDNPLAPAF
                     IAAAMRKAVEAAEVDPGVRIVVFKFFERELANALAHAYERSNTLLVAAGVLPQLRATS
                     KAEAMPAAALPGEAADATGGSGHGQPGGGAATPAMQPNPADQAMFASMLGLLQGWRGS
                     MGPMAGQGGTGPAVTLGSNELVTILSLLQRDLPAQLQLAHRNQEHPLAVQIRQEVVEG
                     ARKLGVESDNLQLDGLDEDAVDLVALLFDVLLDGPQFDTDVRRKIGRMLVPYVKVAVK
                     DRRMFMFKEHPARRLLNTVAEACEGNRGEAPQERELLGHVDSTINRLVAEFNEDVAIF
                     ETLESELRSYMAQHRKRFELAEKRAAEAQRGRERLEQARSSINAELSQHRGERELPAV
                     MDEFLTRYASHHLTQVVLRDGHGSPRHEDAMRAVDDLLVAFDYAELRAPVEELPQLPR
                     EQFEAILASSGCTGDAARGAIESLREALVLLSGGEHAADIAIRMPEQQVSPEPVAAPE
                     AEAVLEIVAGTDKLDFDGSVLDRMRKLQVGSWIQLQTSPERVEPAKVSWISPISGRLL
                     LVNRRGIRVLVASAVELAAMEKLGKVSLRDGETPFEDAMQQVASRLKSAANQA"
     gene            complement(6378..7505)
                     /gene="dnaJ"
                     /locus_tag="PK818_07070"
     CDS             complement(6378..7505)
                     /gene="dnaJ"
                     /locus_tag="PK818_07070"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012438731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HQY82530.1"
                     /translation="MSKRDYYEVLGVARNASDEEVKKAYRRSAMKHHPDRNPGDKGAE
                     AAFKECKEAYEVLSDGGKRRLYDQHGHAAFEHGMGGGNAGPGFHDVGDIFGDIFGNIF
                     GGGGGRQAPRRGADVGYVMELDLEEAVAGIEKRIEIPSLAECQPCKGSGSADGKIDTC
                     GTCHGRGQVRMQRGPFAMQAPCPHCGGSGKAIANPCQTCDGAGRVEEDKTLSVKIPAG
                     VDNGDRIRLAGEGEAGPAGSPPGDLYVEVRVRPHPIFERDGDDLHCEVPIRISQAALG
                     DIVRVPTLGGEVELRIPAETQSGRVFRLRDRGVKSVRSRAPGDLYCKVAVETPVNLTP
                     EQRTLLEQFEASFTGEDARRHSPKSATFLDGVRGFWDRMVS"
     gene            complement(7582..>8225)
                     /locus_tag="PK818_07075"
     CDS             complement(7582..>8225)
                     /locus_tag="PK818_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HQY82531.1"
                     /translation="STAEDNQSAVTVHVLQGEREQARYNKSLAKFDLTGIDAAPRGMP
                     QVEVSFDIDANGILHVNAKDKKTGKEQKVEIKAGSGLSDDEIQRMVQDAEAHREEDKK
                     FHELVSARNHADGLVHATRTTIKDNGDKLPGDLIGRAEGAIAELETAMKGDDKGQIEA
                     KSKALEEAGQALFAAVAAAGQQPSGADAGAAPGAGDDVVDAEFTEVKDDNSKA"
BASE COUNT         1106 a         2823 c         2836 g         1460 t
ORIGIN      
        1 tcggccaggt cgatcgaatc cggcgaggtg ccgatgatcg gcacgccgtt ggcctccagc
       61 gccttcgcca gcttcagcgg ggtctgcccg ccgtactgca cgatcacccc gcgcggcttc
      121 tccagttcca cgatctccag cacgtcttcc agcgtcagcg gctcgaagta caggcggtcg
      181 gaggtgtcgt agtcggtgga cacggtttcc gggttgcagt tgaccatgat ggtctcgtac
      241 ccgtcctcgc gcagggccag cgaggcgtgc acgcagcagt agtcgaactc gatgccctgg
      301 ccgatccggt tcgggccgcc gcccagcacc atgaccttct ggcggtcgct tggctgcgcc
      361 tcgcattcgt cctcgtaggt cgagtacagg taggcggtgg tggtcgcgaa ctcggccgcg
      421 cagctgtcaa cccgcttgta caccgggcgc acgccgaacg ccttgcgcag cgcgcgcacc
      481 gccgattcgt tggtgccggt gagctgggcg atgcgggtgt cggagaagcc cttgcgcttc
      541 agcgcgcgca ggcgcttcgc atcgaggcca tccagcccgc ggtgggcgat gtccacctcg
      601 gcatcgatga gtccttcgat ctggtcgagg aaccacggat ccacgaacga cagcgcgtgc
      661 acgtcctcca ccgacatgcc ggcgcggaac gcgtcggcaa ggtggaagat gcgttccggg
      721 ccgggttcct tcagttcacg gcgcagggtg gcgaggtcgc cctcgtcgac caggtccagg
      781 ccggtcgggt cgagcccgac cttgccggtc tccagcccgc gcagcgcctt gtgcagcgat
      841 tcctggaagg tgcggccgat cgccatcacc tcgcccaccg acttcatctg ggtggtcagg
      901 cgcgcgtcgg cggcggggaa tttctcgaac gcgaagcgcg ggatcttggt gaccacgtaa
      961 tcgatcgccg gctcgaagct ggccggggtc ttgccgccgg tgatctcgtt cttgagttca
     1021 tccagcgtgt agccgaccgc gagcttggcc gcgaccttgg cgatcgggaa gccggtcgcc
     1081 ttcgacgcca gcgccgacga acgcgacacc cgcgggttca tctcgatcac caccacccgc
     1141 ccgttgctgg cgttgatgcc gaactgcacg ttgctgccgc cggtgtccac gccgatcttg
     1201 cgcagcaccg cgatgctcgc gttgcgcagg cgctggtatt ccttgtcggt cagggtctgc
     1261 gccggggcga cggtgatgct gtcgccggtg tgcacgccca tcgcgtcgag gttctcgatg
     1321 ctgcacacga tgatgcagtt gtccgcggtg tcgcggacca cttccatctc gaattccttc
     1381 cagccgagca ccgattcctc gatcagcact tcgctggtcg gcgacagctc caggccgcgg
     1441 ttgacgatct cgatgagttc ctcgcggttg taggcgatgc cgccgccgct gccgccgagg
     1501 gtgaagctgg gccggatgat ggtgggatag ccaacgcggg tctggatgtc gagcgcctgt
     1561 tccagcgaac gcgcgacctc cgccttcggg cattccagcc cgatctcggc catcgccacc
     1621 cggaacagtt cgcggtcttc ggccatgcgg atcgcgtcgc gcttggcgcc gatcaactcg
     1681 acgttgtatt tctcgagtac gccgtggtcg gccaggtcca gcgcgcaatt cagcgcggtc
     1741 tgcccaccca tggtcggcag cagcgcgtcg ggccgttcct tggcgatgat cttctcgacc
     1801 gtctgccagt tgatcggctc gatgtagacg gcatcggcca tctccgggtc ggtcatgatc
     1861 gtcgccggat tgctgttgac cagcaccacc cggtagccct cgtcgcgcag cgccttgcac
     1921 gcctgtgcgc cggaatagtc gaactcgcag gcctggccga tcacgatcgg gccggctccg
     1981 atgatcagga tggtcttgat gtcggtgcgc tttggcatct cagcgggccc ctgcgtgcgt
     2041 gttcgccatc aggccaacga atttgtcgaa gagcggcgcc acgtcgtgcg ggccggggct
     2101 ggcttccggg tgtccctgga agctgaaggc cggtgcatcg gtcagttcga tgccctggtt
     2161 gctgccatcg aacagcgagc gatgggtgac ccgcgcgttc gcgggcaacg tcgtctcgtc
     2221 gatgcagaag ccgtggttct gcgaggtgat catcacccgg ccgctgtcga tgtcgatgac
     2281 cggatggttc gcgccatggt ggccgtgcgg catcttcatg gtctgcgcac cggccgccag
     2341 gccgagcaac tggtggccca ggcagatgcc gtaggtgggg atcttcgcgg cgatgaactc
     2401 gcggattgcg gcgatcgcgt agtcgcaggg ggccgggtcg ccggggccgt tggacaggaa
     2461 cacgccatcg ggctgcagtg ccagtacatc ggcggcaggc gtctgcgccg gcaccaccgt
     2521 cacgtcgcaa ccgcgttcgg ccagcatgcg caggatgttg tgcttggcgc cgaagtcgta
     2581 ggcgacgacc ttgtgccggt gatcgacgcg gttgaatgtc ccgctgtcga ggtcgagctc
     2641 gccgtcgtgc caggggtacg cggcaggcgt gctcaccacc ttcgccaggt ccatcccctt
     2701 caggccgggg aacttgcgcg cggcctcgat cgccttgtcg gcgtcaaggc gttcactcac
     2761 cgtggtgccg gccagcagcg cgccattctg ggcgccgccg tcgcgcagga tgcgggtcag
     2821 cttgcgggtg tcgatgtcgg cgatggcgac gatgccgcgc tgctgcagcc actccggcaa
     2881 cgcgacctgg ctgcgccagt tgctgggccg gcgcggcacg tcgcgcacga tcaggcccgc
     2941 ggcccaggcc tggccggctt cgtcgtcctg ctcggtgcag ccggtgttgc cgatatgcgg
     3001 ataggtcagc gtcaccagtt gccgcgcgta cgacggatcg gtgaggattt cctggtagcc
     3061 ggtcatggcg gtgttgaaca ccacttcgcc cacggacagc ccggcggcgc ccacggaggt
     3121 gccctcgaag acggtgccgt cttcaagcgc gaggatggcg ggtatgttca cgggattcgc
     3181 ctgcgtcagg ttgcagaaaa gcgccgaagc ggcgggggcc ggttcggagc tggggtatcg
     3241 ggcgagcggg aattgtacgg ggtcgtgcct gcctgcgcca gcttcagagc aggtcggcga
     3301 tgcggtggcg ccccgctgcg cggccgtgga tcgcgcgcgc aacgtgcagt gcgccgcggg
     3361 cgaagatgtc gcggttgctt gcgcgatgga ccagctcgat gcgctcgccg gcggtggcga
     3421 actgcaccag gtgctcgccg acgatgtcgc cggcgcggat cgaggcatag cgcgggctgg
     3481 cgccgccctg cgcggccgcg gcacccagcg tcagcgcggt accggagggg gcgtcgagct
     3541 tgcgcacgtg gtgcgtttcg aggatgtcgc agtcccagcc cggcagcgcg cgcgcggcac
     3601 gctcgacgag ctcgtgcagc agcgcgaccc cgaggctgaa attgctggcc cacgccaccg
     3661 cgatggtgcc ggcggcggcg tcgagcgcgg caagctgctg cgtcgacagc ccggtggtgc
     3721 ccgacaccag cggcttgccg cgggacaggc acaggccgag gaccgcgtcg aagccctgcg
     3781 gcaggctgaa atcgatcgcg acatcgaatt cggggacgcc ggcgagttcg ctggccgcga
     3841 actgcgggat gccctcgacg acccgcgccc cggcctggcg cgagaccgcg gcgaccacct
     3901 ggcagcccgc ggttgcatcg cccagccgct gcagggcctg gcccatgcgg ccgttgccgc
     3961 catgcaccag cagtcggatc gttgcttcgc tcatgcccgc aggctagcgg cggcggcgcg
     4021 cgccgacaag cggcgcgcat gcctgcgtcc ggtcaggcct ggttggctgc gctcttcagc
     4081 ctgcttgcga cctgctgcat cgcgtcctcg aacggcgttt cgccgtcgcg cagcgagacc
     4141 ttgccgagct tctccatcgc cgccagttcc accgcggacg ccaccagcac gcggatgccg
     4201 cggcggttga ccagcagcag gcgcccggag atcggactga tccacgagac cttggccggc
     4261 tccacccgtt ccggcgacgt ctgcagctgg atccagctgc cgacctgcag cttgcgcatg
     4321 cggtcgagca cgctgccgtc gaagtcgagc ttgtcggtgc cggccacgat ctccagcacc
     4381 gcctccgcct cgggcgcggc cacgggttcc ggcgacacct gctgctcggg catgcggatc
     4441 gcgatgtctg cggcgtgttc gccaccggaa agcagcacca gggcttcccg cagcgactcg
     4501 atcgcgccgc gggcggcgtc gccggtgcag ccggagctgg cgaggatggc ctcgaactgc
     4561 tcacgtggca gctgcggcag ctcttccacc ggcgcgcgca gttcggcgta gtcgaacgcg
     4621 accagcaggt cgtcgaccgc gcgcatcgcg tcctcgtggc gcggcgagcc gtggccgtcg
     4681 cgcagcacga cctgtgtgag gtggtgggag gcgtagcggg tcaggaattc gtccatcacc
     4741 gccggcagtt cgcgttcgcc gcggtgctgc gagagttcgg cattgatcga gctgcgcgcc
     4801 tgctcgaggc gttcgcggcc gcgctgggct tccgcggcgc gcttctcggc cagctcgaag
     4861 cgcttgcgat gctgcgccat gtaggagcgc agttcggact cgagggtctc gaagatcgcc
     4921 acgtcctcgt tgaactcggc gaccaggcgg ttgatggtgc tgtcgacgtg gccgagcagt
     4981 tcgcgttcct gcggcgcctc gccacggttg ccttcgcagg cttcggcgac ggtgttgagc
     5041 aggcgccgcg ccggatgttc cttgaacatg aacatgcggc ggtccttcac cgcgaccttg
     5101 acgtacggca ccagcatgcg gccgatcttg cggcggacgt cggtgtcgaa ctgcggaccg
     5161 tcaagcagca cgtcgaacag cagcgcgacc aggtccaccg cgtcttcgtc gagtccatcc
     5221 agttgcaggt tgtcgctctc caccccgagc ttgcgcgcgc cctccaccac ctcctggcgg
     5281 atctgcacgg cgagcgggtg ttcctggttg cgatgggcga gctgcagttg cgccggcagg
     5341 tcgcgctgca gcagcgacag gatcgtcacc agttcattcg aacccagggt gaccgccggg
     5401 ccggtgccac cctgcccggc catcggtccc atcgacccgc gccagccctg cagcaggccc
     5461 agcatgctgg cgaacatggc ctggtcggcg gggtttggct gcattgcggg cgttgcggcg
     5521 ccgccacccg gctggccatg accggacccg ccggtggcgt ctgccgcctc gccgggcagc
     5581 gcggctgcag gcatcgcctc ggccttgctg gtggcacgca actgcggcag cacgcctgcg
     5641 gcaaccaaca gcgtattgct gcgctcgtac gcgtgcgcca gcgcattcgc cagttcgcgc
     5701 tcgaagaatt tgaagaccac gatgcgcacg ccgggatcca cctcggcggc ctccacggcc
     5761 ttgcgcatgg cggctgcgat gaacgccggc gccagcgggt tgtcggtggc cttgagttcg
     5821 tcgcgaccaa gcacgtgggc gatgcgcttg cccagcagct ccaggctcgg cgcatgcatg
     5881 cgcgacatgc tttccgccag ttgctcgttg gccagttgct cttccagcgc gtcttccgac
     5941 agcaggctga ggccggaatg ctcgccaagc atggcgctgg ccgcgcgcgg gtcgcgcagg
     6001 gcgcggaatc cgctgatcag tgcctgctcg aagcgctgcg cgatctgcga gcggacccgc
     6061 cgcaggtcgc gcagcgcggt caggccgagg ttgtcgttgc cgctctggct gcggtcgaac
     6121 aggtaatcgt cgacccgccc gagggcgcgg tcgagcgcag gcaccacgcg ttcgatcgcc
     6181 tgcttctgca gggcatccag cagcaggccg ggatccgtgc ggccagcatc ggcgaagtca
     6241 gtgggattgc ggaaggtcat ggggcacctg gagggtgatt ccggcacctt acagcctatg
     6301 cccagctacg tcacatttcg ttgacagcgg tcatgtttcg acacagcgcg gcctgctgca
     6361 aacgaatgca gcaccgctca ggacaccatc cggtcccaga aaccgcgaac cccgtcaagg
     6421 aatgtcgcgg acttcggcga atgccggcgc gcatcctcac cggtgaagct cgcctcgaac
     6481 tgctccagca aggtgcgctg ctcgggggtg aggttgaccg gggtttccac cgcgaccttg
     6541 cagtacaggt cgccgggtgc gcggctgcgc acggacttga cgccgcggtc gcgcagccgg
     6601 aacaccctgc cgctctgggt ctccgcgggg atccgcaatt cgacctcgcc acccagggtg
     6661 ggtacccgca cgatgtcgcc cagcgcggcc tgcgagatcc ggatcggcac ctcgcaatgc
     6721 aggtcgtcgc cgtcgcgctc gaagatcgga tgcgggcgca cgcgcacctc cacgtagagg
     6781 tcgcccggtg ggctgccggc agggccggcc tcgccttcgc ccgccaaccg gatgcggtcg
     6841 ccgttgtcca cgccggccgg gatcttcacc gacagggtct tgtcttcctc gacgcggccg
     6901 gcgccatcgc aggtctggca cgggttggcg atcgccttgc cgctgccgcc gcaatgcggg
     6961 catggcgcct gcatcgcgaa cggcccgcgc tgcatgcgca cctggccgcg gccgtggcag
     7021 gtgccgcagg tgtcgatctt gccgtccgcc gaaccgctgc ccttgcacgg ctggcactcg
     7081 gccagggagg ggatctcgat ccgcttctcg atgccggcga cggcctcctc gaggtccagt
     7141 tccatcacgt agccgacatc ggcgccgcgg cgcggggcct ggcgcccgcc gccgccgccg
     7201 aagatgttgc cgaagatgtc gccgaagatg tcgccgacgt cgtggaaacc ggggccggcg
     7261 ttgccgccac ccatgccgtg ctcgaacgcg gcgtggccgt gctggtcgta cagccggcgc
     7321 ttgccgccgt cgctcagcac ttcataggct tccttgcatt ccttgaacgc ggcctcggcg
     7381 cccttgtcgc cggggttgcg gtccgggtgg tgcttcatcg cgctgcgacg gtaggccttc
     7441 ttgacctcct cgtcgctggc attgcgggcc acgcccagca cttcgtagta gtcgcgtttg
     7501 ctcatcggcc ttgcctgctt ccttcacggc aacgggcaga ccactggcct gcccgctgca
     7561 tgggtcccgc cgctcgcgcg atcaggcctt gctgttgtcg tccttgacct cggtgaactc
     7621 ggcgtcgacc acgtcgtcgc cggcccccgg ggccgcgccc gcatcggcgc cggacggctg
     7681 ctggccggcc gcggccaccg ccgcgaacag cgcctgcccg gcctcttcca gtgccttcga
     7741 cttggcctcg atctggccct tgtcgtcgcc cttcatggcc gtctccagct cggcgatggc
     7801 gccttcggcg cgcccgatca ggtcgcccgg caacttgtcg ccgttgtcct tgatcgtggt
     7861 gcgggtggcg tggaccaggc catcggcgtg gttgcgcgcg ctgaccagtt catggaactt
     7921 cttgtcttcc tcgcggtgcg cctcggcgtc ctgcaccatc cgctggatct cgtcgtccga
     7981 cagcccggag ccggccttga tctcgacctt ctgctccttg ccggtcttct tgtccttggc
     8041 gttcacgtgc aggatgccgt tggcatcgat gtcgaagctc acctcgacct gcggcatgcc
     8101 gcgcggggcg gcgtcgatcc cggtcaggtc gaacttcgcc agcgacttgt tgtagcgggc
     8161 ctgctcgcgt tcgccctgca gcacgtgcac ggtcaccgcg gactggttgt cctcggcggt
     8221 gctga
//
LOCUS       DAORUK010000089         8098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_9897_length_8098_cov_18.608106, whole genome shotgun sequence.
ACCESSION   DAORUK010000089 DAORUK010000000
VERSION     DAORUK010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9897_length_8098_cov_18.608106"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /gene="rlmJ"
                     /locus_tag="PK818_07080"
     CDS             complement(<1..843)
                     /gene="rlmJ"
                     /locus_tag="PK818_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005919277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HQY82532.1"
                     /translation="MNYRHAFHAGNHADVLKHVVLLALCDALTAKPAACFALDTHAGR
                     GLYRLDGESAQKTGEASDGIGRLLAEAPRNPPIQRYLAAIKACRQQHGAHAYPGSPWL
                     LAHALRAQDRISACELLPEEANVLRDTFAGDPRVAVHARDGYAAVKALLPPRHGTVKF
                     ARGLVLVDPPYEAQLDEFDTALAAIRDVLARWPQAMLALWYPIKQRRALRQFYRKAAG
                     LDARSTLLLELLVRPDDSPLRMNGSGMLLLNAPWQFDVAMRPALDGLCAALGEAGGSA
                     RVEWL"
     gene            920..1606
                     /gene="creB"
                     /locus_tag="PK818_07085"
     CDS             920..1606
                     /gene="creB"
                     /locus_tag="PK818_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HQY82533.1"
                     /translation="MPMTILLAEDEGAIAETVLYALRAEGYAAEHVLLGGEVLPRLRR
                     GGIDLVILDVGLPDTSGFDVCRALRIESEVPVLFLTARDAELDRVLGLELGGDDYMAK
                     PFSPRELVARVRARLRRQVVHDDTAGWRRLGRFELDATGHRLRFDGVLLDLTRYEFGL
                     LEALLARPGAVLDRARLMDRVWGDALESGERTVDTHVKTVRAKLRAIAPDADPIRTVR
                     GLGYAVHVEG"
     gene            1610..>2952
                     /gene="creC"
                     /locus_tag="PK818_07090"
     CDS             1610..>2952
                     /gene="creC"
                     /locus_tag="PK818_07090"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF008312.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HQY82534.1"
                     /translation="MNIGLRVLLGYFLIVALAALLVGKVFVEQVKPGVRQAMEGTLID
                     TANTLAELASDDMLAGRIADGRFARRLRAVQARKLDADVWGFAKDRSGFRVTVTDARG
                     IVVFDSEGKDLGRDNSRWNDVYRTLRGEYGARSTREDPGDEASTVMHVAAPIRDDQGR
                     IVGVLTVAKPNRAMAPFIARSQQIVARWGLVLMGAALLVGIAAAWWLSRQLGALRRYA
                     DAVAAGERAPQPAAAGEFGDLGRALHDMREKLEGKQYVEQYVHALTHELKSPLAAIRG
                     ATELLEGPLPDADRQRFVASIATQGTRMAQTVDKLLALAAVEHRQRIEQPQALDAAQL
                     LREAVDAVRAHADGAGIGLQVDAADAPGLSGDPFLLRQALVNLLDNALDFAPPGTQVE
                     ARARRDGDGVRFEIADRGPGVPDYARDRVFERFYSLPRPHGGSRSSGLGLPFVAQV"
     assembly_gap    2953..3052
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3427..4818
                     /gene="creD"
                     /locus_tag="PK818_07095"
     CDS             3427..4818
                     /gene="creD"
                     /locus_tag="PK818_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004150404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HQY82535.1"
                     /translation="MRLGLKIAMVVVLVLAILIPLAMIRGTIAERQQYRQQAVDEVTR
                     SYAGEQGLAGPVLVVPYREQVEVEERDATGVLRKQVREVDRQWLFFPKTMHVHGRVLP
                     SIRKRGLHQVRVYEWQGSIDAAFDVRLPDADPARPRTLGAPWLDIGIADVRGLVGAPT
                     LRVAGAATPILQGQKGRGGGGVHAVLPAALVAGERIAFPVQFGFALRGTEALAIVPLA
                     DTNRIVLDSPWPHPQFNGDFLPRTHRIDGKGFHAEWDVSSLASNAQAQYREGGAAMGK
                     ATPAPGHDGVAAAVEASASIDRVGLSLVEPVNLYSKVDRASKYGLLFVLLTFVGFFLF
                     ETIKQLPIHPIQYALVGLALAIFFLLLLSLSEHIAFGLAYLAAALACIGLIGYYVGHV
                     LRSRTRGMGFAAMLGLLYAALYGLLMSEDNALVLGAGLLFVVLAAIMVATRKVDWYQV
                     GARIGAPSAPTSL"
     gene            4839..5441
                     /locus_tag="PK818_07100"
     CDS             4839..5441
                     /locus_tag="PK818_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017914663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY82536.1"
                     /translation="MHDAISLESLSGAALLPHLDDVARLRIAVFRDWPYLYEGDVGYE
                     RAYLAAYAQSADSVVVLARAGDALIGASTGIPLAHDSAGFQAPFRARGIDVAQVFYCG
                     ESVLLPAWRRRGIGHAFFDAREAHARRLGGFGWTAFAAVDRGDADPRRPPGHRGNEAF
                     WARRGYVRQPGMTMHLHWNELAIGDVTHPLTFWLRPLETP"
     gene            5438..6355
                     /locus_tag="PK818_07105"
     CDS             5438..6355
                     /locus_tag="PK818_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017914662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HQY82537.1"
                     /translation="MKVAVAKYPIGEPRDFEAFAARQEALLREAKAGGARLAVLPEYL
                     ALELAAMFAAATRSDLHASLAALQPLRGAWMALYTRLAREMGMWINAGSFLLDGGHGR
                     YRNRSDVFAPDGGHLWQDKLRLTGFEKDTGVIESGDALKVFEVDGVRAGIAICYDSEF
                     PLPVRAQCEAGARLLLVPSCTDTEAGATRVRVGCLARALENRCFVAHAVTTGAAAWSP
                     ALDSNTGEAALLAPMDVGLPDDGILAQTRGDEVWAIAELDVSALDASRAHAQVANDRD
                     WPAQYFPSVLRARLERAQGHAPEQRDARA"
     gene            6425..8023
                     /locus_tag="PK818_07110"
     CDS             6425..8023
                     /locus_tag="PK818_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQY82538.1"
                     /translation="MKRLLLTAALLAAGVAGLPGAFAQEKVDLDMTSRIRQEAFQRSQ
                     AMATLNHLTDVIGPRLTNSPSMAEANRWTRSKFTEWGLSNVHDEAVDDIGRGWSFNDA
                     GVEMLSPRVMPLHALPKAWTPGTAGPVEGEAIAAKLQKKEDLEKWKGKLKGKVVFTDD
                     LRAYKPNEKSDFDRHDHESLEELLSYPLPSERTRDASMLAQYRERMEFGPLLNKFLID
                     EGVVATMSISSWNDGIVRVMGGGSRKAGESVGVPALVMMAEHYNTVMRALERKETVKL
                     RVNVDARFLNDDNQPGYNTIAEIPGSGPDRNEVVMLGAHMDSWHTGSGANDNGAGVAV
                     MMEAVRILKAVGARPNRTIRVALWTGEEQGLIGSQAYVAKHFASYPEPTDPEQKALPA
                     AFRSNKGKLAKTADYGKITAYFNLDNGSGKIRGIYAQENLAIAPIFEEWMKPWNDVGA
                     TIVTQRNTGSTDHVSFDRVGIPGFQFVQDQLDYFSHVHHTHLDVQDHAVADDLKQASA
                     IVASFAYNAAQRPAKLPRKMMVED"
BASE COUNT         1204 a         2679 c         2930 g         1185 t
ORIGIN      
        1 cagccactcc acgcgcgccg acccgccggc ctcgcccagc gccgcgcaca ggccgtcgag
       61 tgccggccgc atcgccacgt cgaactgcca cggcgcgttc aacagcagca tcccgctgcc
      121 gttcatgcgc agcggcgaat cgtccgggcg caccagcagc tccagcagca gggtggacct
      181 ggcatccagc ccggccgcct tgcggtagaa ctggcgcagc gcgcggcgct gcttgatcgg
      241 gtaccacagc gcgagcatcg cctgcggcca gcgcgccagc acgtcgcgga tcgcggccag
      301 ggcggtgtcg aattcgtcca gctgcgcctc gtagggcggg tcgaccagga ccagcccgcg
      361 cgcgaacttc accgtcccat gccggggcgg caacagcgcc ttgaccgcgg cgtagccgtc
      421 gcgcgcgtgc accgccaccc gcgggtcgcc ggcgaaggtg tcgcgcagca cgttggcttc
      481 ctcgggcagc agctcgcagg cggagatccg gtcctgcgcg cgcagcgcat gcgccagcag
      541 ccacggcgaa ccggggtagg catgcgcgcc gtgctgctgc cggcacgcct tgatcgcggc
      601 caggtagcgc tggatcggcg ggttccgcgg ggcctcggcg agcagcctgc cgatgccgtc
      661 gctggcctcg ccggtcttct gcgcggattc gccatccagc cggtacaggc cgcggccggc
      721 atgggtgtcg agtgcgaaac aggcggccgg cttggcggtg agcgcatcgc acagcgccag
      781 cagcacgaca tgcttgagca cgtcggcgtg gttgccggca tggaaggcgt ggcgatagtt
      841 catcgcggca ttgtcgcatc gcgcgccact tcacacccgc aacgcaattg ccgcggcgtc
      901 ctcggctagg atgcggcgca tgccgatgac catcctgctg gccgaagacg agggcgcgat
      961 cgccgagacc gtgctgtacg cgctgcgtgc cgaaggctat gccgccgagc acgtgctgct
     1021 gggcggcgag gtcctgccgc ggctgcggcg cggcggcatc gacctggtga tcctcgacgt
     1081 gggcctgccg gacaccagcg gcttcgacgt ctgccgcgcg ctgcgcatcg aaagcgaagt
     1141 gccggtgctg ttcctgaccg cgcgcgacgc cgagctggac cgcgtgctgg ggctggaact
     1201 cggtggcgac gattacatgg ccaagccgtt ctccccgcgc gagctggtgg cgcgggtgcg
     1261 cgcgcggctg cgccggcagg tggtccatga cgacaccgct ggctggcgga ggctcgggcg
     1321 cttcgaactg gacgccaccg gccatcggct gcgcttcgat ggcgtgttgc tggacctgac
     1381 ccgctacgaa ttcggcctgc tggaggcatt gctggcgcgc cctggcgcgg tgctggaccg
     1441 ggcgcggctg atggaccggg tgtggggcga tgcgctggaa tcgggcgagc gcacggtcga
     1501 tacccatgtg aagaccgtgc gcgcgaaact gcgcgccatc gccccggacg cggacccgat
     1561 ccgcaccgtg cgcgggctgg gctatgccgt gcatgtcgag ggctgagcgg tgaacatcgg
     1621 cctgcgcgtg ctgctgggct acttcctgat cgtggcgctg gccgcgctgc tggtcggcaa
     1681 ggtgttcgtc gagcaggtca agccgggcgt gcgccaggcg atggaaggca ccctgatcga
     1741 caccgccaac accctggcgg agctggccag cgacgacatg ctggccgggc gcatcgccga
     1801 cggccgcttc gcccggcgcc tgcgcgcggt gcaggcgcgc aagctcgacg ccgacgtctg
     1861 gggcttcgcc aaggaccgca gcggtttccg ggtgacggtg accgatgcgc gcgggatcgt
     1921 ggtgttcgac tcggagggca aggacctcgg ccgcgacaac tcgcgctgga acgacgtcta
     1981 ccgcaccctg cgcggcgagt acggcgcgcg ttccacccgc gaggatccgg gcgacgaggc
     2041 gagcacggtg atgcacgtgg ctgcgccgat ccgggacgac caggggcgga tcgtcggcgt
     2101 gctgaccgtg gccaagccga accgggcgat ggcgccgttc atcgcccgca gccagcagat
     2161 cgtggcccgc tggggcctgg tgctgatggg tgccgcgctg ctggtgggga tcgccgccgc
     2221 atggtggctg tcgcgccagc tgggcgcgct gcgccgctac gcggatgcgg tggcagccgg
     2281 cgaacgtgcg ccgcagccgg cggcggccgg cgaattcggc gacctcgggc gtgccctgca
     2341 cgacatgcgc gagaaactgg aaggcaagca gtacgtcgaa cagtacgtgc acgcgctgac
     2401 ccacgaactg aaaagcccgc tggcggcgat ccgcggggcc actgagctgc tggaaggccc
     2461 gttgccggac gccgaccgcc agcgtttcgt cgccagcatc gccacccagg gcacgcgcat
     2521 ggcgcagacg gtcgacaagc tgctggcgct ggcggcggtg gaacatcgcc agcgaatcga
     2581 gcagccgcag gcgctggatg cggcgcagtt gctgcgcgag gcggtggacg ccgtgcgcgc
     2641 gcatgccgat ggcgccggca tcggcctgca ggtggatgcg gcggatgcgc cgggattgtc
     2701 cggcgatccc ttcctgctgc ggcaggcgct ggtcaacctg ctcgacaacg cactggattt
     2761 cgccccgccg ggaacgcagg tcgaggcgcg ggcgcggcgc gatggcgatg gcgtccgctt
     2821 cgaaatcgcc gatcgcggcc ccggcgtgcc cgactacgcg cgcgaccgcg tgttcgagcg
     2881 cttctactcg ctgccgcgcc cgcatggcgg cagccgcagc agcgggctgg gcctgccgtt
     2941 cgtggcgcag gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcccgcat
     3061 ggcggcagcc gcagcagcgg gctgggcctg ccgttcgtgg cgcaggtggc agagctgcat
     3121 ggtggtagtg cggaacttcg tgagcgcgac ggcggcggca cgatagcaac gctattgctt
     3181 ccgctctgac ctccctcacg cgaaaaactc cggggcagcg tcggtcggct gggtagcgcc
     3241 cgagcgcgcc atggatggcg cgcgcccgcg agatcagtcc tggacgactg gaccgagcgg
     3301 cgggcgctac ccagccgacc gacgatgccc gcgacgcacc ccacttcacc cacgcttcat
     3361 cgccagcacc acgacggctc actggcgcgc gccagcatgc cctctccaca ggaagggaga
     3421 ggcgtcatgc gcttgggctt gaagatcgcg atggtggtcg tgctggtgct ggcgatcctg
     3481 attccgctgg cgatgatccg cggcaccatc gccgagcggc agcaataccg ccagcaggcg
     3541 gtggacgagg tcacccgcag ctatgccggc gaacagggcc tggccgggcc ggtgctggtg
     3601 gtgccgtatc gcgagcaggt ggaggtcgag gaacgcgacg ccactggcgt gctgcgcaag
     3661 caggtgcgcg aggtcgaccg ccaatggctg ttcttcccga agacgatgca cgtgcatgga
     3721 cgcgtgctgc cctcgatccg caagcgcggc ctgcaccagg tgcgggtcta cgaatggcag
     3781 ggcagcatcg atgccgcctt cgacgtgcgt ctgcccgacg ccgatccggc gcgtccgcgc
     3841 acgctcggcg cgccgtggct ggacatcggc atcgccgacg tgcgcggcct ggtcggcgca
     3901 cccaccctgc gcgttgccgg cgccgccacg ccgatcctgc agggccagaa ggggcgcggc
     3961 ggtggcggcg tgcatgcggt actgcctgcg gcgctggtcg ccggcgagcg catcgcgttc
     4021 cccgtgcagt tcggtttcgc cctgcgcggc accgaggcgc tggcgatcgt gccgctggcc
     4081 gacaccaacc gcatcgtgct ggattcgccg tggccgcatc cgcagttcaa cggcgatttc
     4141 ctgccgcgca cgcatcgcat cgacggcaag ggtttccatg ccgaatggga cgtgtcctcg
     4201 ctggccagca acgcgcaggc gcaataccgc gaaggcggcg cggcgatggg caaggccact
     4261 cctgcgccgg gtcacgatgg cgtcgccgcc gcggtggagg cgtcggccag catcgacagg
     4321 gtcgggctgt cgctggtcga gccggtcaac ctgtattcca aggtcgatcg cgccagcaag
     4381 tacgggttgc tgttcgtgct gctgaccttc gtcggcttct tcctgttcga gaccatcaag
     4441 cagttgccga tccacccgat ccagtacgcg ctggtcgggc tggcgctggc gatcttcttc
     4501 ctgctgctgc tcagcctgtc cgagcacatc gcgttcggcc ttgcctacct ggctgcggcg
     4561 ctcgcctgca tcgggctgat cggctactac gtcggccatg tcttgcgcag ccgcacgcgc
     4621 gggatgggct tcgcggcgat gctcggcctg ctgtacgccg cgctgtacgg cctgctgatg
     4681 tcggaggaca acgcgctggt gctgggcgcg ggcctgctgt tcgtggtgct ggccgcgatc
     4741 atggtcgcta cccgcaaggt ggactggtac caggtggggg cgaggatcgg cgcgccctcc
     4801 gcgccgacgt cgctctagcc tgtcgataga atcggcgcat gcacgacgcg atctccctcg
     4861 agagcctatc cggtgcggcc ctgctgccgc acctggacga tgtggccagg ctgcgcatcg
     4921 cggtgttccg cgattggccc tacctctacg aaggcgacgt gggctacgaa cgcgcgtacc
     4981 tggccgcgta cgcgcaatcg gcggacagcg tggtggtgct ggcgcgcgcc ggcgatgcgc
     5041 tcatcggcgc cagcaccggg attccgctgg cccatgacag cgccggattc caggcgccgt
     5101 tccgcgcgcg cggcatcgac gtggcgcagg tcttctactg cggcgagtcg gtgttgctgc
     5161 cggcgtggcg caggcgcggc atcggccatg ccttcttcga tgcccgcgag gcgcatgcgc
     5221 gcaggctggg cgggttcggc tggaccgcgt tcgccgcggt cgatcgcggt gacgcggatc
     5281 cgcggcgccc gcccgggcat cgcggcaacg aggcgttctg ggccaggcgt ggctacgtgc
     5341 gccagccggg aatgaccatg cacctgcact ggaacgagct cgccatcggc gatgtgacgc
     5401 atccgctcac attctggctg cggccactgg agacgccatg aaagtcgcag tcgcgaagta
     5461 cccgatcggc gaaccgcgcg atttcgaggc gttcgcggcc aggcaggagg ccctgctgcg
     5521 ggaggcgaag gccggcggtg cgcggctggc ggtgctgccg gaatacctgg cgctggagct
     5581 ggcggcgatg ttcgcggcag cgacgcgcag cgacctgcat gcgtcgctgg ccgcgctgca
     5641 gccgctgcgc ggtgcgtgga tggccttgta cacgcggctg gcgcgcgaga tgggcatgtg
     5701 gatcaacgcc ggcagcttcc tgctcgacgg cgggcatggc cgctaccgca accgcagcga
     5761 cgtgttcgca ccggacggcg gacacctgtg gcaggacaag ctgcggctga ccgggttcga
     5821 gaaggacacc ggggtgatcg agtccggcga tgccttgaag gtgttcgagg tcgatggcgt
     5881 gcgcgccggg atcgcgatct gctacgacag cgaattcccg ttgccggtgc gtgcgcagtg
     5941 cgaggccggc gcgcgcctgc tgctggtgcc cagttgcacc gataccgaag ccggcgcgac
     6001 ccgcgtgcgg gtcggttgcc tggcgcgcgc gctggagaac cgctgtttcg tcgcccacgc
     6061 ggtgaccacc ggcgcggcag cgtggagccc ggcgctggac agcaataccg gcgaggcggc
     6121 gttgctggcg ccgatggacg tgggcctgcc ggatgacggg atcctggcgc agacgcgcgg
     6181 cgacgaggtg tgggccatcg ccgaactgga cgtgtccgcg ctggacgcaa gccgcgccca
     6241 cgcccaggtc gccaacgacc gcgactggcc ggcgcaatat ttcccgagcg tgctgcgggc
     6301 gcggctggaa cgtgcgcaag gtcacgcgcc ggagcaacgc gacgcccgtg catgaccgat
     6361 gggctaggcg ccgggcacgc gtgcacgcct agactcgggg tttgagcctg ccccggggat
     6421 tgcgatgaag cgattgttgt tgacggccgc gttgctcgcg gcgggtgttg ccggtttgcc
     6481 gggcgcgttc gcgcaggaga aggtcgacct ggacatgacc tcgcgcatcc gccaggaggc
     6541 gttccagcgc tcgcaggcga tggcgaccct gaaccacctg accgacgtga tcggcccgcg
     6601 cctgaccaat tcgccgtcga tggcggaagc caaccgctgg acccggtcga agttcaccga
     6661 atggggcctg tccaacgtgc acgacgaagc ggtggacgac atcggccgcg gctggtcgtt
     6721 caacgacgcc ggggtggaga tgctgagccc gcgggtgatg ccgctgcacg cgctgccgaa
     6781 ggcgtggacc cccggcaccg ccggcccggt cgagggcgag gcgatcgccg ccaagctgca
     6841 gaagaaggaa gacctggaga agtggaaggg caagctgaag ggcaaggtgg tgttcaccga
     6901 cgacctgcgc gcctacaagc cgaacgagaa gtccgacttc gaccgccacg accatgagag
     6961 cctggaggag ctgctttcgt acccgctgcc gagcgagcgc acgcgcgatg ccagcatgct
     7021 ggcgcagtac cgcgaacgca tggaattcgg gccgctgctg aacaagttcc tgatcgacga
     7081 gggcgtggtc gccacgatgt cgatcagcag ctggaacgac ggcatcgtcc gggtgatggg
     7141 cggcggttcg cgcaaggccg gcgagtcggt cggcgtgccg gcgctggtga tgatggccga
     7201 gcactacaac acggtgatgc gcgcgctgga gcgcaaggag acggtgaagc tgcgcgtgaa
     7261 cgtggatgcg cgcttcctca acgacgacaa ccagccgggc tacaacacca tcgccgagat
     7321 ccccggcagc ggcccggaca ggaacgaagt ggtgatgctg ggcgcgcaca tggattcctg
     7381 gcacaccggt tccggcgcca acgacaacgg cgccggcgtg gcggtgatga tggaggcggt
     7441 gcgcatcctg aaggcggtcg gcgcgcggcc caaccgcacc atccgcgtcg cgctgtggac
     7501 cggcgaggag cagggcctga tcggctcgca ggcatacgtg gccaagcact tcgccagcta
     7561 tccggagccg accgatccgg agcagaaggc gttgccggcc gcgttccgca gcaacaaggg
     7621 caagctggcg aagaccgcgg actacgggaa gatcaccgcc tacttcaacc tcgacaacgg
     7681 cagcggcaag atccgcggga tctatgcgca ggaaaacctg gcgatcgcgc cgatcttcga
     7741 ggaatggatg aagccgtgga acgacgtcgg cgcgaccatc gtcacccagc gcaacaccgg
     7801 cagcaccgac cacgtcagct tcgaccgcgt cggcatcccg ggcttccagt tcgtgcagga
     7861 ccagctggac tacttcagcc acgtccacca cacccacctg gacgtgcagg accacgcggt
     7921 ggccgacgac ctcaagcagg catcggcgat cgtggcctcg tttgcctaca acgcggcgca
     7981 gcgcccggcg aagctgccgc gcaagatgat ggtcgaggat tgatcgacac caggatctga
     8041 tgcacgaagg gccggcaatg ccggcccttc ctttttgtgg ttcctcgcgc cacgccgg
//
LOCUS       DAORUK010000090         8064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_9953_length_8064_cov_17.960919, whole genome shotgun sequence.
ACCESSION   DAORUK010000090 DAORUK010000000
VERSION     DAORUK010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8064
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9953_length_8064_cov_17.960919"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /gene="kdsA"
                     /locus_tag="PK818_07115"
     CDS             <1..458
                     /gene="kdsA"
                     /locus_tag="PK818_07115"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HQY82539.1"
                     /translation="GKPVNIKKGQFLSPWDMKPVVEKARSTGNQQIMVCERGASFGYN
                     NLVSDMRSLSVMRETGCPVVFDATHSVQLPGGQGASSGGQREFVPVLARAAVAVGVSG
                     VFMETHPDPGKALSDGPNAWPLGRMEALLETLLELDAATKRNGFLESSL"
     gene            478..1764
                     /gene="eno"
                     /locus_tag="PK818_07120"
     CDS             478..1764
                     /gene="eno"
                     /locus_tag="PK818_07120"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQY82540.1"
                     /translation="MSTITHVHAREILDSRGNPTLEAEVTLADGSFGRAMVPSGASTG
                     TKEAVELRDGDKTRYLGKGVQKAVANVNGAIADMLAGFDAADQAGLDKRLIDLDGTEN
                     KARLGANALLAVSMANAHAVAASRKMPLWQHLANGNAVSLPVPMMNIINGGAHADNNV
                     DLQEFMVLPVGFDSFSEALRAGTEIFHALKSVLKGHGLSTAVGDEGGFAPDLRSNVEA
                     LDTILEAIGKAGYKAGEDVLLGLDVASSEFFENGKYNLTGEGKRLTSEQFVDFLANWA
                     AQYPIVTIEDGMAEHDWAGWKQLTERLGRTVQLVGDDLFVTNPRIFRDGIAQGVANAI
                     LIKVNQIGTLTETLEAIAIADAANYAAIVSHRSGETEDTTIADIAVATTATQIKTGSL
                     CRSDRVAKYNQLLRIEQALGAGARYAGRGAFVSLAR"
     gene            1795..2127
                     /gene="ftsB"
                     /locus_tag="PK818_07125"
     CDS             1795..2127
                     /gene="ftsB"
                     /locus_tag="PK818_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HQY82541.1"
                     /translation="MRPLHIVILLLALLLALLQYKLWLGSGGEREVAALRAQVARQES
                     ENQRLQLRNEALKAEVEDLKSGEAAVEERARSELGMVRPGETFYRVIEDRAGSPSVPA
                     APAPGAPR"
     gene            2124..2834
                     /gene="ispD"
                     /locus_tag="PK818_07130"
     CDS             2124..2834
                     /gene="ispD"
                     /locus_tag="PK818_07130"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HQY82542.1"
                     /translation="MTPGPPRAGAWALLPAAGSGRRFGGEVPKQYLAAAGKPLVEHAL
                     DALLAHPAVDGVVVALAAGDARWPGWTRLHGKPVIACVGGGERADSVLAALCALPDAV
                     AGDALLLVHDAARPNLRAADITALIEAAQACADGAILAAPVRDTLKRATDDARIAGTE
                     PRDGLWRALTPQAFRRDLLLRALRHARAQGLVVTDEAMAVERLGLHPALVEGREDNLK
                     VTTPADLALAEVLLANAT"
     gene            2831..3313
                     /gene="ispF"
                     /locus_tag="PK818_07135"
     CDS             2831..3313
                     /gene="ispF"
                     /locus_tag="PK818_07135"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HQY82543.1"
                     /translation="MTKIRIGQGFDVHAFGDGDHVMLGGVRVPHTRGVLAHSDGDVAL
                     HALCDAILGALALGDIGQHFPPTDPRWKDADSRALLRQCVALVHARGWRVGNCDLTIL
                     CEQPKVGPHAQAMREAIAAGLGIEVEAVGVKATTTEALGFLGRGEGIAAQAACLLVHG
                     "
     gene            3310..4344
                     /gene="truD"
                     /locus_tag="PK818_07140"
     CDS             3310..4344
                     /gene="truD"
                     /locus_tag="PK818_07140"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HQY82544.1"
                     /translation="MTELARAHGVPVLAARIRGTPEDFFVEELPGFEPSGAGEHLLLT
                     VEKRGMNTAFAAKRIAEWAGVAESAIGYAGIKDRHAVTRQAFSVWIPKKVAPAIDALQ
                     SDELQVLGHAWHSRKLPRGALAGNRFVLVLRGIEGEREAIEQRLQAISMRGVPNYFGE
                     QRFGRGGGNVQQAVAMFAGRRARREERTMLLSAARSELFNRVLAARVDAGNWDAALDG
                     EVWMLDGSRSVFGPEALTDELQARLAAFDIHPSGPLWGGGELRSSGAVREIELVAVHG
                     DTASRLRDGLERAGLKQERRALRLRPRELAWRWLDVAALELAFALPPGCYATTVMREL
                     GTITDAATPL"
     gene            complement(4422..4982)
                     /locus_tag="PK818_07145"
     CDS             complement(4422..4982)
                     /locus_tag="PK818_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HQY82545.1"
                     /translation="MRKKPAIEALPEADDAALFRAAIGADRGKVRALPEAPLPPAAPK
                     PKPSAKMARRDEDAARDEFRHVLVAALEAGDLLSHRREDVPQPVLRRLARGEYAAQEE
                     LDLHGLPARAAEALLREFLRDCRAHGVGCVRIVHGKGRNSEERLPVLKNLVDRVLRHR
                     ADVLAFHSPPAAQGGSGAVLVLLEKR"
     gene            5054..5833
                     /gene="surE"
                     /locus_tag="PK818_07150"
     CDS             5054..5833
                     /gene="surE"
                     /locus_tag="PK818_07150"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HQY82546.1"
                     /translation="MRVLVSNDDGVDAPGIRALASGLREAGHEVLVVAPDRDRSGASN
                     SLTLDAPIRVVQVDEATWKVYGTPTDCVHVAITGMFEVEPDIVVSGINNTANLGDDVI
                     YSGTVAAAMEGRFLGLPAVAMSLVTADHHGRHYETAARAAVEIIARLRTDPLPADTIL
                     NVNVPDLPWDEIRGFETSRLGHRHRAEPCTPQKDPRGRQWWWIGAAGQEADAGPGTDF
                     NAVRSGHIAITPIHVDLTRYQALEQVASWVGGLAETLGRRP"
     gene            5830..6510
                     /locus_tag="PK818_07155"
     CDS             5830..6510
                     /locus_tag="PK818_07155"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HQY82547.1"
                     /translation="MISRLRLQPADVGMGMTSQRVRDRLIDRLRANGIVDERVLNAIR
                     TVPRHQFVDEALATRAYEDTALPIGHGQTISQPWVVAKMTEALLETAPKRVLEIGTGS
                     GYQGAVLAALGLEVFTVERIGELLRTARKRFRNLGLHVRSKHDDGRIGWPEEAPFDGI
                     IVTAAAPALVDALSEQLAPGGTLVAPVGGTSGQSLLRLRKHADGNIEQSDLGPVIFVP
                     LLSGMTDQ"
     gene            6507..7121
                     /locus_tag="PK818_07160"
     CDS             6507..7121
                     /locus_tag="PK818_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HQY82548.1"
                     /translation="MKLFGPMYERTLRWARHPRATWLLALLSFIEAIFFPIPPEVMLA
                     PMCLSQPRRAFWFASVSLASAMVGAVIGYYLGHFAWQAVQPLLAALGMLDSIEHGIEI
                     VREKSAHSPWAVFWFLVLAGFSPIPMKVFTWASGIVGVPMLPFMLSMLVGRGKRLYIV
                     AAAIRIGGERAERTLHRYIEPIGWGALALLAAAIGWLALRGHAA"
     gene            7130..7897
                     /locus_tag="PK818_07165"
     CDS             7130..7897
                     /locus_tag="PK818_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQY82549.1"
                     /translation="MRLYRIAIACCTLALLAACGRSHVTREPAANASAGERPVRVSQP
                     KYGATVVVQRGQNVYRIATENGLTALDLALWNDIPPPYTIHPGQRLRLYPGGQRGVAT
                     RPAARPPAKPGAPVVAKPAAPAPVVIPATSNIGWRWPADGVVVARFANGDPTRQGVDI
                     AGKGGQPVRAAADGVVVYSGSGLVGYGELVILKHSDQWLSAYGHNRARLVNEGQLVKA
                     GQQIAEMGRSGASRDMLHFEIRYNGKPVDPQQYLPKR"
BASE COUNT         1211 a         2778 c         2932 g         1143 t
ORIGIN      
        1 ccggcaagcc ggtcaacatc aagaagggcc agttcctgtc gccgtgggac atgaaaccgg
       61 tggtggagaa ggcaaggtcc accggcaacc agcagatcat ggtctgcgag cgtggcgcat
      121 cgttcggcta caacaacctg gtcagcgaca tgcgcagcct gtcggtgatg cgcgaaaccg
      181 gctgcccggt ggtgttcgac gccacccact cggtgcagtt gccgggcggg cagggcgcaa
      241 gctccggcgg ccagcgcgaa ttcgtgccgg tgctggcgcg cgccgcggtc gccgtgggcg
      301 tgtccggcgt gttcatggaa acccatcccg acccgggcaa ggcgctgtcc gacggtccca
      361 atgcgtggcc gctgggcagg atggaagcgc tgctggaaac actgctggaa ctcgatgccg
      421 cgaccaagcg caacggcttc ctcgaatcct ccctctgacc gcacaagacc ccatccgatg
      481 agcacgatca cccacgtcca cgcccgcgaa atcctcgaca gccgcggcaa tcccaccctt
      541 gaagccgaag tgacgctggc cgatggcagc ttcggtcgcg cgatggtgcc gtccggcgcg
      601 tccaccggca ccaaggaggc ggtggagctg cgcgacggcg acaagacccg ctacctcggc
      661 aagggcgtgc agaaggcggt ggccaacgtc aacggcgcga tcgccgacat gctggcgggc
      721 ttcgatgcgg ccgaccaggc cgggctcgac aagcgcctga tcgacctcga cggcaccgag
      781 aacaaggcgc ggctcggcgc caacgcactg ctggcggtct ccatggcgaa cgcgcatgcg
      841 gtggccgcct ccaggaagat gccgctgtgg cagcacctgg cgaacggcaa cgcggtctcg
      901 ctgccggtgc cgatgatgaa catcatcaac ggcggcgcgc acgcggacaa caacgtcgac
      961 ctgcaggaat tcatggtgct gccggtcggc ttcgattcgt tctcggaagc gctgcgcgcc
     1021 ggcaccgaga tcttccacgc gctgaagtcg gtgctgaagg gccacggcct gtccaccgcg
     1081 gtcggcgacg agggcggctt cgcacccgac ctgcgcagca acgtggaagc gctggacacg
     1141 atcctggaag cgatcggcaa ggccggctac aaggcgggcg aggacgtgct gctgggcctc
     1201 gacgttgcct ccagcgagtt cttcgagaac ggcaagtaca acctcaccgg cgaaggcaag
     1261 cgcctgacct cggagcagtt cgtcgacttc ctcgccaact gggcggcgca gtacccgatc
     1321 gtcaccatcg aggacggcat ggccgagcac gactgggccg gctggaagca gctcaccgag
     1381 cgcctcggca ggaccgtgca gctggtcggc gacgacctgt tcgtgaccaa cccgcggatc
     1441 ttccgcgacg gcatcgcgca gggcgtggcc aacgcgatcc tgatcaaggt caaccagatc
     1501 gggaccctga ccgaaaccct ggaagccatc gccattgccg acgcggcgaa ctacgcggcg
     1561 atcgtctccc accgttccgg cgagaccgag gacaccacca tcgccgacat cgcggtggcg
     1621 accaccgcga cccagatcaa gaccggttcg ctgtgccgtt ccgaccgcgt cgccaagtac
     1681 aaccagctgt tgcgcatcga gcaggcgctg ggcgccggcg cccgctacgc cgggcgtggc
     1741 gcgttcgtgt cactggcgcg ctgagcgcgg cggccgggcg aggaacccat gcgcgtgcgg
     1801 ccgttgcaca tcgtgatcct gttgctggcg ctgctgcttg cgctgctgca gtacaagttg
     1861 tggctgggca gcggcggcga gcgcgaggtc gccgcgctgc gcgcgcaggt ggcgcgccag
     1921 gaatccgaaa accagcgcct gcagttgcgc aacgaggcgc tgaaggcgga agtggaagac
     1981 ctgaagtccg gcgaagccgc ggtggaggaa cgcgcgcgca gcgaactcgg gatggtcagg
     2041 cccggcgaaa ccttctaccg ggtgatcgag gaccgcgccg gctcgccgtc agtccccgcg
     2101 gcgccggcgc cgggcgcgcc acggtgaccc cgggcccgcc cagggccggt gcgtgggcgc
     2161 tgctgcccgc cgccggcagc ggccgccgct tcggtggcga ggtgcccaag caatacctgg
     2221 ccgccgccgg caagccactg gtcgaacatg cgctggatgc gttgctggcg catccggcgg
     2281 tcgatggcgt ggtggtggcg ctggcggcag gcgatgcgcg ctggccgggt tggacacggc
     2341 tgcatggcaa gccggtgatc gcctgcgtgg gcggcggcga gcgcgccgat tcggtgctgg
     2401 ccgcgttgtg cgcattgcct gacgcggtcg ccggagacgc cctgctgctg gtccacgacg
     2461 ccgcgcgccc gaacctgcgc gccgcggaca tcaccgcact gatcgaagcc gcgcaggcct
     2521 gcgccgatgg cgcgatcctc gcggcaccgg tgcgcgacac cctgaagcgc gccaccgatg
     2581 acgcgcggat cgccggcacc gaaccgcgcg acggcctctg gcgcgcgctg accccgcagg
     2641 cgttccgccg cgacctgctg ttgcgtgcgc tgcggcacgc gcgcgcgcaa ggcctcgtcg
     2701 tcaccgacga ggcgatggcg gtcgagcggc tcggcctgca tccggcgctg gtcgaaggcc
     2761 gcgaggacaa cctgaaagtc accacccccg ccgaccttgc gttggccgaa gtcctgctgg
     2821 cgaacgcgac atgaccaaga tccggatcgg gcagggcttc gacgtgcacg cgttcggcga
     2881 cggcgaccac gtgatgctcg gcggcgtgcg ggtgccgcac acgcgcggcg tgctcgccca
     2941 cagcgacggc gacgtggccc tgcacgcgct gtgcgatgcg atcctcggtg cactggcgct
     3001 gggcgacatc ggccagcact tcccgccgac cgatccacgc tggaaggatg cggacagccg
     3061 cgccctgctg cgccagtgcg tggcgctggt ccacgcgcgc ggctggcgcg tcggcaactg
     3121 cgacctcacc atcctttgcg aacaaccgaa agtcggcccg catgcgcagg ccatgcgcga
     3181 ggcgattgcc gccggcctcg gcatcgaagt cgaggcggtc ggcgtcaagg ccaccaccac
     3241 cgaggcgctg ggcttcctcg gccgcggcga aggcatcgcc gcgcaggcag cctgcctgct
     3301 ggtgcacgga tgaccgaact ggcgcgggcg catggcgtgc cggtgctggc cgcgcgcatc
     3361 cgcggcacgc ccgaagattt cttcgtcgag gagctgccgg gcttcgagcc cagcggcgcc
     3421 ggcgagcacc tgctgctgac cgtcgagaag cgcggcatga acaccgcgtt cgcggcgaaa
     3481 cggatcgccg aatgggcggg cgtggccgag tcggcgatcg gctacgccgg catcaaggac
     3541 cgccacgcag tgacccggca agcgttctcg gtgtggatcc cgaagaaggt ggcgccggcg
     3601 atcgatgcgc tgcagtccga cgaactccag gtgctcgggc atgcgtggca ttcgcgcaag
     3661 ctgccgcgcg gcgcgctggc gggcaaccgc ttcgtgctgg tgctgcgtgg catcgaaggc
     3721 gagcgggagg ccatcgaaca gcgcctgcag gccatctcga tgcgcggcgt gccgaactac
     3781 ttcggcgagc agcgtttcgg gcgcggcggc ggtaacgtgc agcaggcggt ggcgatgttc
     3841 gccgggcgcc gtgccaggcg cgaggaacgc accatgctgc tgtcggccgc gcgttcggag
     3901 ctgttcaacc gggtgctggc ggcgcgcgtc gatgcaggca actgggacgc cgcgctcgat
     3961 ggcgaggtgt ggatgctgga tggcagccgc agcgtgttcg ggccggaggc gctgaccgac
     4021 gaactgcagg cgcggctggc ggcattcgac atccatccga gcggtccgct gtggggcggg
     4081 ggcgagctgc gcagcagcgg tgcggtgcgc gagatcgagc tcgtggccgt gcacggcgac
     4141 accgcctcgc gcctgcgcga tggcctggaa cgcgcggggc tgaagcagga acggcgcgca
     4201 ctgcggctgc gtccgcggga gctggcttgg cgctggctcg atgttgccgc gcttgagctg
     4261 gccttcgcgc taccgcccgg ctgctatgcg accacggtca tgcgcgagct tggcaccatc
     4321 acggacgctg ccacgccgct gtaggaacgg cgcaagccgc gacctccacg ccggtcgcgg
     4381 cttgcgccgc tctgcaggat gcggccgggt ccaggcgccg atcagcgttt ctccagcagc
     4441 accagcaccg caccgctgcc accctgcgcg gcgggcggcg aatggaaggc gagcacatcc
     4501 gcgcggtgcc gcagcacccg gtccaccagg ttcttcagca ccggcaaccg ttcttccgaa
     4561 ttgcggccct tgccatggac gatccgcacg cagcccacgc cgtgcgcgcg gcaatcgcgc
     4621 aggaactcgc gcaacagggc ttccgcggcg cgcgctggca ggccgtgcag gtccagttcc
     4681 tcctgtgccg cgtattcgcc gcgggccagt cgccgcagca ccggctgggg cacgtcctcg
     4741 cgccgatggc tgagcaggtc gccggcctcc agcgcggcga ccagcacgtg ccggaattcg
     4801 tcgcgcgcgg cgtcctcgtc gcggcgggcc atcttcgccg acggcttcgg cttcggcgct
     4861 gccggcggca gcggggcctc tggcagggcg cggaccttgc cgcgatcggc gccgatcgcg
     4921 gcgcggaaca gggccgcgtc gtcggcttcc ggaagcgctt cgatggcagg tttttttcgc
     4981 accggcgcag ggtaacgcag cggccttccg ctatcatgcc cgcatgcaca atgttccgat
     5041 gccgtagggc gccatgcggg tcttggtcag caacgacgat ggcgtcgacg cccccggcat
     5101 acgtgccctc gccagcggcc tgcgcgaggc cggccacgag gtgctggtgg tggcgccgga
     5161 ccgcgaccgc agcggcgcca gcaactcgct gacgctggat gcgccgatcc gggtggtgca
     5221 ggtcgacgag gcgacgtgga aggtctacgg caccccgacc gactgcgtgc acgtggcgat
     5281 caccggcatg ttcgaggtcg agccggacat cgtggtgtcc ggcatcaaca acaccgccaa
     5341 cctcggcgac gacgtgatct attcgggcac cgtcgccgcc gcgatggagg gccgtttcct
     5401 cggcctgccg gcggtggcga tgtcgctggt caccgccgac caccacggcc gccactacga
     5461 aaccgcggcg cgcgcggcgg tggagatcat cgcccgcctg cgcaccgacc cgctgccggc
     5521 cgacaccatc ctcaacgtca acgtgccgga cctgccgtgg gacgagatcc gcggcttcga
     5581 gaccagccgg ctgggccatc gccatcgcgc cgaaccgtgc acgccgcaaa aggatccgcg
     5641 tggccgccag tggtggtgga tcggcgccgc cggccaggag gccgatgccg gcccgggcac
     5701 cgatttcaat gccgtgcgca gcggccacat cgcgatcacc ccgatccacg tcgacctgac
     5761 ccgctaccag gcgctggagc aggtggcgag ctgggtcggt ggcctggccg agacgctggg
     5821 gcgcaggcca tgatctcgcg cctgcgcctg cagccggccg atgtcggcat gggcatgacc
     5881 agccagcgcg tgcgcgaccg cctgatcgat cgcctgcgcg cgaacggcat cgtcgatgag
     5941 cgggtgctca acgcgatccg cacggtgccg cgccaccagt tcgtcgacga ggcgctggct
     6001 acccgcgcgt acgaggacac cgcgttgccg atcggccatg ggcagaccat ctcgcagccg
     6061 tgggtggtgg cgaagatgac cgaagccctg ctggagaccg cgccgaagcg ggtgctggag
     6121 atcggcaccg gttccggcta ccagggcgcg gtgctagccg cgctggggct tgaagtcttt
     6181 accgtggagc gcatcggcga gctgctgcgc accgcgcgca agcgcttccg caacctcggc
     6241 ctgcacgtgc gcagcaagca tgacgacggc cgcatcggct ggccggagga ggcgccgttc
     6301 gacggcatca tcgtcaccgc cgccgcgccg gcgctggtgg atgcgctgag cgagcagctg
     6361 gcgcccggcg ggaccctggt ggcgccggtg ggtggcacct ccgggcagtc gctgctgcgc
     6421 ctgcgcaagc acgcggacgg caacatcgag cagtcggacc tcgggccggt gatcttcgtg
     6481 ccgctgctgt cggggatgac cgaccaatga agctgttcgg cccgatgtac gagcgcaccc
     6541 tgcggtgggc gcgccacccc agggcgacat ggttgctggc cttgctcagc ttcatcgagg
     6601 cgatcttctt cccgatcccg ccggaggtca tgctggcgcc gatgtgcctg tcgcagccgc
     6661 ggcgcgcgtt ctggttcgcc agcgtgagcc tggccagcgc gatggtcggc gcggtgatcg
     6721 gctattacct cggccatttc gcctggcagg cggtgcagcc gctgctggcc gcgctcggga
     6781 tgctggattc gatcgagcac gggatcgaga tcgtgcgcga gaagagcgcg cattcgccat
     6841 gggcggtgtt ctggttcctg gtgctggccg ggttctcgcc gatcccgatg aaggtcttca
     6901 cctgggccag cggcatcgtt ggcgtgccga tgctgccgtt catgctcagc atgctggtcg
     6961 ggcgcggcaa gcgcctgtac atcgtggcgg cggcgatcag gatcggcggc gaacgcgccg
     7021 agcggacctt gcaccgctac atcgagccga tcggctgggg cgcactggcg ctgctggccg
     7081 cggccatcgg ttggctggcg ttgcgggggc acgcggcatg atcggcggca tgcgcctgta
     7141 ccgcattgcc atcgcctgct gcacgctcgc gctgctggcc gcctgcgggc gcagccacgt
     7201 gacccgcgag cccgcggcga atgcatccgc cggcgagcgg ccggtgcggg tctcgcagcc
     7261 gaagtacggc gcgacggtgg tggtgcagcg cgggcagaac gtgtaccgca tcgccaccga
     7321 aaacggactg accgcgctcg acctggcgct gtggaacgac atcccgccgc cctacaccat
     7381 ccatcccggg cagcgcctgc gcctgtaccc gggcgggcag cgcggggtgg cgacgcggcc
     7441 ggccgcgcgg ccgccggcga agcccggtgc gccggtggtt gcgaagccgg ccgcaccggc
     7501 gccggtggtc attcccgcga ccagcaacat cggctggcgc tggccggcgg atggggtggt
     7561 ggtggcgcgc ttcgccaatg gcgatcccac ccggcagggc gtcgacatcg ccggcaaggg
     7621 cgggcagccg gtgcgcgccg ccgccgacgg ggtggtggtg tattccggtt ccggcctggt
     7681 cggctacggc gagctggtga tcctcaagca cagcgaccag tggctgtccg cctatggcca
     7741 caaccgcgcg cgcctggtca acgagggcca gctggtgaag gccggccagc agatcgccga
     7801 gatgggccgc agcggcgcgt cgcgcgacat gctgcatttc gagatccgct acaacggcaa
     7861 gccggtcgat ccgcagcagt atttgccgaa gcggtagaga cttcgaaagc gatgagcatg
     7921 gccgccgggg tactgtcccg agcgcgccat ggatggcgcg cgcccgcgaa ctgagtcgtg
     7981 gatgactcga gtgagcggcg gggcagtacc ccggcggcca cgcggagcct ggcgccagcg
     8041 gctatgccgc gaggatgaac cccg
//
LOCUS       DAORUK010000091         8052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_9977_length_8052_cov_17.149931, whole genome shotgun sequence.
ACCESSION   DAORUK010000091 DAORUK010000000
VERSION     DAORUK010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9977_length_8052_cov_17.149931"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PK818_07170"
     CDS             <1..914
                     /locus_tag="PK818_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY82550.1"
                     /translation="TLMTAMVSLVPMLRQLTNVQSMLQRGISSAERLFSVMDAPSERD
                     QGTRPLQRARGLIEFHEVDARYEGQDAPALHAISFVARPGTVTAIVGRSGSGKSTLAK
                     LIPRFYEAERGQILLDGHPLADYPLADLRRQIALVGQQVMLFDGSVADNVAYGEMQEA
                     APERIEAAVRGANAMEFVANLPDGVATHIGAKGGKLSGGQRQRLAIARAMLKDAPILI
                     LDEATAALDNESERLVQDALAQLIPDRTTLVIAHRLSTIEHADQVLVLDAGRLVEQGT
                     HAELLARGGVYAHLHAMQFREGNGQPA"
     gene            911..1930
                     /gene="lpxK"
                     /locus_tag="PK818_07175"
     CDS             911..1930
                     /gene="lpxK"
                     /locus_tag="PK818_07175"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006449592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HQY82551.1"
                     /translation="MSRRRLPQAPAWWYDGSPPPLHARLLSTLYGGLTALRRGAYRRG
                     WLHKQRVAVPVVVVGNITAGGTGKTPLVIALVARLRAAGWTPGIASRGHGREHEATPR
                     WVDADAAASLVGDEPLLIARATAAKVRVDRDRVAAARALVAAGCDIVVCDDGLQHYRL
                     ARDIEIEVQDARRRYGNGRLLPAGPLREPVERAADCGFRVLNLGVAGEGVAARPPGLG
                     EWPMRLQVGAVQPLHGGRPQPLAMFAGQRVHAVAGIGDPERFFAMLRDAGIAVVPHAF
                     PDHHAYAAPDFDFGSRLPVLMTAKDAVKCAGLVDAQAFMVPVEAVLPEPFWLALLDRL
                     PARSR"
     gene            2093..2872
                     /gene="kdsB"
                     /locus_tag="PK818_07180"
     CDS             2093..2872
                     /gene="kdsB"
                     /locus_tag="PK818_07180"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HQY82552.1"
                     /translation="MTSTDFVVAIPARYAASRLPGKPLRLIGGEPMVLHVARRALAAG
                     AREAWVATDDARIADALDGSGVQVAMTSATHASGSDRLAECAGIAGWGDDTIVVNLQG
                     DEPFAPADGIACVARAVAASGAGIATLAAPIDDIETLLDPNVVKVVLAGSGDALYFSR
                     APVPWPRDAFAVDRTRMPSGRWLRHIGIYGYRASALRAFAALPAGDLERTESLEQLRA
                     LEAGWRIAVRLAPSPFPPGVDTEEDLARAQDMHRMQRGIDA"
     gene            2884..3351
                     /locus_tag="PK818_07185"
     CDS             2884..3351
                     /locus_tag="PK818_07185"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HQY82553.1"
                     /translation="MGVLMVCLGNICRSPMAEGVLLARLQEAGLGGKVRVDSAATGRS
                     HIGHAPDERAIACAKRHGIDIAAQRARALQADDFDAFDFILCADRTILHDARMRKPRT
                     AHGDVELLLEWSGVGRKDVPDPYYGDARDFEKAFKLVDAAALGVVERIRQGRF"
     gene            3376..5265
                     /gene="uvrC"
                     /locus_tag="PK818_07190"
     CDS             3376..5265
                     /gene="uvrC"
                     /locus_tag="PK818_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HQY82554.1"
                     /translation="MSGHQPGAEAEAFDGKAVAAAMPAAPGVYRMTAADGTVLYVGKA
                     RALRNRVGSYFNATPKPTRIMAMLAQVARIEITVTRSEADALLLENQLIKSLQPRYNV
                     LLRDDKSYPFVLLTREQWPRLAFHRGPRALPGRYFGPYPGATSVRETLNLMHKLFRLR
                     SCEDSVFRNRSRPCLQHQIGRCSAPCVGLVEVDEYADAVRRAGLFLDGRSGELTDELT
                     TAMQQASDALEFEQAARLRDLVGAIRKLQSRQYVDGRNADLDVLAVAMQGALACVLML
                     AFRDGRNLGTRAFFPKTNGEDSAAAVLAAFVSQYYAEQPPPAEIVLDRDIPERELIER
                     ALAEHAGRKVELRSSVRGERAAHLDLVRRNAELALATELTSSSAQQARLDGLRDLLGL
                     VEAPKRIECFDISHTMGEATVASCVVFDAAGPVRKQYRRYNIGGIEPGDDYAAMHQAL
                     QRRFRPPTEGEPEAVLPDVLLIDGGAGQVAQAQAVLQESGVVGVAVVGVAKGPARKAG
                     AETLLLPDGREVRPGAASPALQLVQQVRDEAHRFAITGHRGKRQKARNSSRLEDIAGI
                     GPRRRAALLRHFGGLSGLKAAAVDDIARVEGVNRALAERIWANLHGLGQGALEPGAAQ
                     EQEQG"
     gene            5262..5846
                     /gene="pgsA"
                     /locus_tag="PK818_07195"
     CDS             5262..5846
                     /gene="pgsA"
                     /locus_tag="PK818_07195"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HQY82555.1"
                     /translation="MKLTIPTWLTLARIAMIPVLVVVYYLDYRWTNIAATAIFVFASL
                     TDWLDGWIARRYHQFSAFGAFLDPVADKLMISTALVITVQYHHTIWMALWASVIIGRE
                     IAVSALREWMAELGQRAKVAVATVGKVKTVVQMVALGFLLYREPLLGLPIFQIGEWLL
                     AAAALLTLWSGFEYARAAWPALSRQEAQGVDTPE"
     gene            5870..5945
                     /locus_tag="PK818_07200"
     tRNA            5870..5945
                     /locus_tag="PK818_07200"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5903..5905,aa:Gly,seq:gcc)
     gene            6005..6078
                     /locus_tag="PK818_07205"
     tRNA            6005..6078
                     /locus_tag="PK818_07205"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6037..6039,aa:Cys,seq:gca)
     gene            6087..6162
                     /locus_tag="PK818_07210"
     tRNA            6087..6162
                     /locus_tag="PK818_07210"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6120..6122,aa:Gly,seq:gcc)
     gene            6240..6326
                     /locus_tag="PK818_07215"
     tRNA            6240..6326
                     /locus_tag="PK818_07215"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6274..6276,aa:Leu,seq:taa)
     gene            6352..7977
                     /locus_tag="PK818_07220"
     CDS             6352..7977
                     /locus_tag="PK818_07220"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HQY82556.1"
                     /translation="MSRYTAYVASIVLAFGALALAQWHPHAWWAVAVFAGLALLGTLD
                     MLQRKSTLRRNFPILAHFRYGLETIGPEMRQYFIQSDTERVPFSREQRALIYQRAKHA
                     NDVRPFGTLQDVYGVDYEWINHSMAPAEVANHDFRITIGEGCAQPYSASVFNISAMSF
                     GALSAAAIRALNGGAKRGGFYHDTGEGSISPYHREAGGDLVWELGSGYFGCRNHDGSF
                     NARRFTEQARDAQVKMIEVKLSQGAKPGHGGVLPAAKLSAEIAATRDVPMGQDCVSPA
                     RHSAFSTPLGLLQFVAHLRELSGGKPTGFKLALGHPWEWFGIAKAMHESGLLPDFIVV
                     DGAEGGTGAAPAEFVDHVGVPMHEGLMLVHNTLVGVGLRDRIKIGAAGKITSAFDIAR
                     TLAMGADWCNAARGYMFALGCIQSLSCHTNRCPTGIATQDPSRWKHLDVPDKTTRVFE
                     FHDNTLRALRDLLCAAGLSHPDQLGPEHILRRVSPTEVRSLAARYRYLAPGVLLRGED
                     PGHAVFRDYWDTARSDAFMPPANVLELRGSKAN"
BASE COUNT         1243 a         2635 c         2883 g         1291 t
ORIGIN      
        1 tgaccctgat gaccgcgatg gtcagcctgg tgccgatgct gcgccagctc accaacgtgc
       61 agagcatgct gcagcgtggc atcagctccg ccgagcggct gttctcggtg atggacgcac
      121 ccagcgaacg cgaccagggc acccgcccgc tgcagcgcgc gcgcgggctg atcgagttcc
      181 acgaggtcga cgcgcgctac gaaggccagg acgcgccggc gctgcatgcc atcagcttcg
      241 tggcccggcc gggcaccgtc accgccatcg tcggccgctc cggcagcggc aagtcgaccc
      301 tggccaagct gatcccgcgc ttctacgaag ccgaacgcgg gcagatcctg ctcgacggcc
      361 atccgctcgc cgactacccg ctcgccgacc tgcgccggca gatcgcgctg gtcggccagc
      421 aggtgatgct gttcgacggc agcgtggccg acaacgtggc ctacggcgag atgcaggagg
      481 ccgcgccgga gcggatcgag gcggcggtgc gaggcgccaa cgcgatggag ttcgtggcga
      541 acctgccgga cggcgtggcg acccacatcg gcgccaaggg cggcaagctg tccggcggcc
      601 agcgccagcg cctggcgatc gcccgcgcga tgctcaagga cgcgccgatc ctgatcctcg
      661 acgaggccac cgcggcgctc gacaacgagt ccgaacggct ggtgcaggat gcgctggcgc
      721 agctgatccc ggaccgcacc acgctggtga tcgcgcatcg gctgtcgacc atcgaacatg
      781 ccgaccaggt gctggtgctg gatgccggcc ggctggtgga gcagggcacc catgccgagc
      841 tgctggcgcg cggtggcgtg tatgcgcacc tgcatgcgat gcagttccgg gaaggcaacg
      901 ggcaaccggc atgagccggc gccgcttgcc gcaagcgccg gcctggtggt acgacggcag
      961 cccgccaccg ctgcacgcgc gcctgctgtc cacgctctat ggcgggctca ccgcgctgcg
     1021 gcgcggggcc tatcgccgcg gctggttgca caagcagcgg gtggccgtgc cggtggtcgt
     1081 ggtcggcaac atcaccgccg gcggcaccgg caagaccccg ctggtgatcg cactggtcgc
     1141 gcgcctgcgc gccgcgggct ggacccccgg catcgccagc cgcggccacg gccgcgagca
     1201 cgaggcaacg ccacgctggg tggacgccga tgccgccgcc tcgctggtcg gcgacgagcc
     1261 gttgctgatc gcgcgcgcga ccgcggccaa ggttcgggtg gaccgcgacc gggtcgcggc
     1321 cgcgcgcgcg ctggtcgccg ccggctgcga catcgtcgtc tgcgacgatg gcctccagca
     1381 ctaccggctc gcgcgcgaca tcgagatcga agtgcaggac gcgcgtcgtc gctacggcaa
     1441 cggccgcctg ctcccggccg gtccactgcg cgagccggtg gaacgcgcgg cggactgcgg
     1501 gttccgggta ctcaacctcg gcgtcgccgg cgagggcgtg gcagcgcgcc cgccaggcct
     1561 cggcgaatgg ccgatgcggc tgcaggtcgg cgccgtgcag cccctgcacg gcgggcgccc
     1621 gcagccgctg gcgatgttcg ccggccagcg cgtgcatgcg gtggccggga tcggcgaccc
     1681 ggagcgtttc ttcgccatgc tgcgcgatgc cgggatcgcg gtggtgccgc atgcgttccc
     1741 cgaccaccat gcctacgccg cgcccgattt cgacttcggc agccgcctgc cggtgctgat
     1801 gaccgcgaag gacgcggtga aatgcgcggg attggtggat gcgcaggcct tcatggtgcc
     1861 ggtcgaggcg gtgctgccgg agccattctg gctggcgttg ctggatcggt tgccggcgcg
     1921 gagcaggtga ccttcatgtg tcggatcacg atgagggtcg gggtcgtcgg tgcggctggg
     1981 gtgtgcttcg agcgcgccat ggatggcgcg cggccgcgaa tcgtagctgg acgcgaagcc
     2041 tgagcggcga agcacacccc agccgcaccg acgaccggcg ataatccgac ccatgacaag
     2101 caccgatttc gtcgtcgcca ttcccgcccg ctacgcggcc tcgcgcctgc ccggcaagcc
     2161 gctgcgcctg atcggcggcg aaccgatggt cctgcacgtc gcgcggcgcg cgctggccgc
     2221 cggtgcgcgc gaggcctggg tggcgaccga cgacgcccgc atcgctgatg ccctggacgg
     2281 cagcggcgtg caggtcgcga tgacctcggc cacgcacgcc tccggcagcg accgcctggc
     2341 cgagtgcgcc ggcatcgccg gctggggcga cgacaccatc gtggtcaacc tgcagggcga
     2401 cgagccattc gcgccggccg acggcatcgc ctgcgtggcc cgcgcggtcg ccgccagcgg
     2461 cgccggcatc gccaccctgg ccgcgccgat cgacgacatc gaaaccctgc tcgatccgaa
     2521 cgtggtcaag gtggtgctgg ccggcagcgg cgatgcgctg tacttcagcc gcgcgccggt
     2581 gccgtggccg cgcgatgcgt tcgcagtcga ccgcacgcgg atgccgagcg gccgctggct
     2641 gcgccacatc ggcatctacg gttaccgcgc ctccgccctg cgcgcattcg cggcgctgcc
     2701 ggcgggcgac ctggagcgca ccgaatcgct ggaacagctg cgcgcgctgg aggctggctg
     2761 gcggatcgcg gtgcggctgg cgccgtcgcc attcccgccc ggggtggata ccgaggaaga
     2821 cctcgcgcgc gcgcaggaca tgcaccgcat gcagcggggg atcgacgcgt gatgacgagg
     2881 acgctgggcg tgctgatggt gtgcctgggc aacatctgcc gctcgccgat ggcggaaggc
     2941 gtgttgctgg cgcggctgca ggaagccggg ctgggcggca aggtgcgggt ggattccgcg
     3001 gccaccggtc gttcgcacat cggccatgcg ccggacgagc gcgccatcgc ctgcgcgaag
     3061 cggcacggca tcgacatcgc cgcacagcgc gcgcgcgcgc tgcaggcgga cgacttcgac
     3121 gccttcgatt tcatcctctg cgccgaccgc accatcctgc acgatgcgcg catgcgcaag
     3181 ccacgcaccg cgcatggcga cgtggagctg ctgctggaat ggagcggcgt cggcaggaag
     3241 gacgtgccgg atccgtacta cggcgatgcg cgcgatttcg agaaggcatt caagctggtc
     3301 gacgcggcgg cgctgggcgt ggtcgagcgc atccgccagg gacgcttctg agcgcgccgc
     3361 gatgaggccg gacgcatgag cgggcaccag ccgggtgcgg aagccgaggc gttcgacggc
     3421 aaggcggtgg ccgcggcgat gccggccgca ccaggggtgt accggatgac cgccgccgac
     3481 ggcacggtgc tgtatgtcgg caaggcgcgc gcgttgcgca accgcgtcgg cagctatttc
     3541 aacgcaaccc ccaagccgac ccggatcatg gcgatgctgg cgcaggtggc caggatcgag
     3601 atcacggtca cccgcagcga ggcggatgcg ttgctgctgg aaaaccagct gatcaagtcg
     3661 ctgcagccgc gctacaacgt gttgctgcgc gacgacaaga gctatccgtt cgtgctgctg
     3721 acccgcgagc agtggccgcg gctggcgttc caccgcgggc cgcgcgcgct gccggggcgc
     3781 tacttcggcc cgtatccggg cgcgacgtcg gtgcgcgaga cgctgaacct gatgcacaag
     3841 ctgttccgcc tgcgcagttg cgaggacagc gtgttccgca accgctcgcg gccatgcctg
     3901 cagcaccaga tcggccgctg cagcgcgccc tgcgtcggcc tggtggaggt ggacgagtac
     3961 gccgacgcgg tgcgccgcgc gggcctgttc ctggatggcc gcagtggcga actcaccgat
     4021 gagctgacga cggcgatgca gcaggccagc gacgcgctcg agttcgagca ggcggcgcgg
     4081 ttgcgcgacc tggtcggtgc catccgcaag ctgcagtcgc gccagtacgt cgatggccgc
     4141 aatgccgacc tcgacgtgct ggcggtggcg atgcagggtg cgctggcctg cgtgctcatg
     4201 ctcgccttcc gcgacggccg caacctcggc acccgcgcgt tcttcccgaa gaccaatggc
     4261 gaggacagcg ccgccgcggt gctggctgcg ttcgtctccc agtactacgc cgagcaaccg
     4321 ccgccggcgg agatcgtgct cgaccgcgac atccccgaac gcgaactgat cgaacgcgcg
     4381 ctggccgagc acgccgggcg caaggtcgaa ctgcgcagca gcgtgcgcgg cgagcgcgcg
     4441 gcgcacctgg acctggtccg gcgcaacgcg gaactggcgc tggccaccga actgaccagc
     4501 agcagtgcgc agcaggcgcg gctggacggg ttgcgcgacc tgctcgggct ggtggaggcg
     4561 ccgaagcgga tcgaatgctt cgacatcagc cacacgatgg gcgaggcgac ggtggcgtcc
     4621 tgcgtggtgt tcgatgcggc cgggccggtg cgcaagcagt accgccgcta caacatcggc
     4681 gggatcgagc ccggcgacga ctacgcggcg atgcaccagg ccttgcagcg gcgcttccgg
     4741 ccgccgaccg aaggcgagcc cgaagcggtg ctgccggacg tgctgctgat cgacggcggc
     4801 gccgggcagg tggcgcaggc gcaggcggtg ctgcaggaaa gcggtgtcgt cggcgtggcc
     4861 gtggtcgggg tagccaaggg cccggcgcgc aaggcaggtg cggaaaccct gctgctgccg
     4921 gatgggcgcg aagtgcggcc cggcgcggct tcgccggcgc tgcagctggt ccagcaggtg
     4981 cgcgacgagg cgcaccgctt cgccatcacc ggccaccgcg gcaagcggca gaaggcgcgc
     5041 aacagcagcc ggctggagga catcgccggg atcgggccgc gccggcgtgc ggccctcttg
     5101 cggcatttcg gtggtctgtc aggattgaag gccgcggcgg tcgacgacat cgcccgggtc
     5161 gaaggggtca accgtgcgct ggccgaacgc atctgggcga acctgcacgg actggggcag
     5221 ggcgcactgg aaccaggcgc ggcgcaagag caggagcagg gatgaagctg acgataccca
     5281 catggttgac gctggcgcgg atcgcgatga tcccggtgct ggtggtggtc tattacctgg
     5341 actaccgctg gacgaatatc gcggcgaccg cgatcttcgt gttcgcgtcg ctgaccgact
     5401 ggctggacgg ctggatcgcg cggcgctacc accagttctc ggcgttcggt gcgttcctgg
     5461 acccggtggc ggacaagctg atgatctcga ccgcgctggt gatcaccgtg cagtaccacc
     5521 acacgatctg gatggcgctg tgggccagcg tgatcatcgg ccgcgagatc gcggtgtcgg
     5581 ccctgcgcga gtggatggcc gaactcggcc agcgggcgaa ggtggcggtc gccacggtcg
     5641 gcaaggtcaa gaccgtggtg cagatggtgg cgctggggtt cctgctgtac cgcgagcccc
     5701 tgctggggct gccgatcttc cagatcggcg aatggctgct ggcggcggcg gcgctgctga
     5761 ccctgtggtc cggcttcgag tacgcccgcg cggcgtggcc ggccctgagc aggcaggaag
     5821 cgcaaggtgt tgacactccg gaatgaaccc gtaaaatggc gggctcaacg cgggaatagc
     5881 tcagttggta gagcacgacc ttgccaaggt cggggtcgag ggttcgagtc ccttttcccg
     5941 ctccagtttt agcgaagccc cattcgtggg gcttcgctgt ttgagagtgt tgtaccagtc
     6001 atccggccgg gtggcagagt ggttatgcag cggactgcaa atccgcgtac gccggttcaa
     6061 ttccgacctc ggcctccaca attcaagcgg gaatagctca gttggtagag cacgaccttg
     6121 ccaaggtcgg ggtcgagggt tcgagtccct tttcccgctc cagtggtctg aagccccgct
     6181 cacgcggggc tttgtttttt aggtcgattg ggccggccat gacgtaagct tgcatccatg
     6241 cctgggtggc gaaatcggta gacgcaccgg acttaaaatc cgtcggtcgc aagaccatga
     6301 gggttcgagt ccctccccag gcaccattgc agccacgcat ttggaggtgc catgtcgcgc
     6361 tataccgctt atgtcgcaag cattgtgctg gctttcggcg cgttggcact ggcacagtgg
     6421 cacccgcatg cgtggtgggc ggtggcagtg tttgccggat tggcgctgct gggaacgctg
     6481 gacatgttgc agcgcaaaag cacgctgcgc cgcaatttcc cgatcttggc gcatttccgc
     6541 tatgggctgg agactatcgg cccggagatg cggcagtact tcattcagtc cgataccgag
     6601 cgcgtgccgt tttcccgcga gcagcgtgcg ttgatctacc agcgtgccaa acacgccaac
     6661 gacgtgcgtc cgtttggcac cttgcaggac gtgtacggtg tggattacga atggatcaac
     6721 cattcgatgg caccggccga agtggccaat cacgattttc gcatcaccat cggcgaaggc
     6781 tgcgcacagc cgtactcggc cagtgtcttc aatatttccg cgatgagttt cggggcattg
     6841 tcggcggcgg caatccgcgc actcaatggt ggtgccaaac ggggcgggtt ctatcacgac
     6901 accggcgaag gctccatttc gccctatcac cgcgaggccg gcggtgattt ggtgtgggaa
     6961 ctgggctcgg gctattttgg ttgccgcaac catgacggca gcttcaatgc ccggcgcttc
     7021 accgaacagg cgcgcgatgc gcaggtgaaa atgatcgagg tcaagctgtc gcagggtgca
     7081 aaacctggcc acggcggggt gttgccggcg gccaaactca gcgccgaaat tgctgccacc
     7141 cgtgatgtgc cgatgggcca ggattgcgtc tcgccggcgc gtcattcggc attctccacg
     7201 ccgcttgggt tgctgcaatt tgtcgcccat ttgcgtgagt tgtcgggtgg caagccgacc
     7261 ggcttcaaat tggcgctggg gcatccatgg gaatggtttg gcatcgccaa agcgatgcac
     7321 gaaagcggtc tgctgcccga tttcatcgtg gtcgatggcg ctgaaggtgg taccggcgcg
     7381 gccccggcgg aatttgtgga tcatgtgggc gtgccgatgc acgaaggcct gatgctggta
     7441 cacaacacgc tggtgggtgt ggggctgcgg gatcgcatca aaatcggcgc ggctggcaag
     7501 atcaccagcg ccttcgacat cgcgcgcacc ttggcgatgg gcgcggattg gtgcaatgcc
     7561 gcgcgcggct acatgttcgc gctgggttgc atccaatcct tgagttgcca taccaaccgc
     7621 tgccccaccg ggattgccac ccaggacccg tcgcgctgga aacatctgga tgtgcccgac
     7681 aaaacaacgc gcgtgttcga gttccacgac aacaccctgc gcgccctgcg cgacttgctg
     7741 tgcgctgcgg gcttgagtca cccggatcag cttggcccgg aacacatcct gcgtcgggtc
     7801 tcgcccaccg aggtacgctc gctggccgca cgctaccgct atcttgcgcc gggcgtgctg
     7861 ttgcgtggtg aagaccccgg gcatgcggta ttccgcgact attgggacac ggcgcgcagt
     7921 gatgccttca tgccacctgc gaatgtcctg gagctgcgtg ggagcaaggc caactgatac
     7981 tctttgcaac atcaaatacg tgttgttgct gccttgacta tctcactgtc ccatttggat
     8041 cggcttgagg cc
//
LOCUS       DAORUK010000092         7967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_10131_length_7967_cov_18.792846, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000092 DAORUK010000000
VERSION     DAORUK010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10131_length_7967_cov_18.792846"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="PK818_07225"
     CDS             <1..137
                     /locus_tag="PK818_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RlmI/RlmK family 23S rRNA methyltransferase"
                     /protein_id="HQY82557.1"
                     /translation="RRAAFYAGRTVQVLKVAGAGPDHPWLAQVPESRYLKAVFCRVLD
                     "
     gene            148..1485
                     /locus_tag="PK818_07230"
     CDS             148..1485
                     /locus_tag="PK818_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQY82558.1"
                     /translation="MTRLRPLALLLLPLLVGCATYATIGNQPLTSAAAAAPGYDMGQV
                     GVEGRRDDVTLALAFSGGGSRAAALAYGVLLELRETRVGAAREHRLLDEVDAISAVSG
                     GSFTAAYYGLHDEATFRDFEPGFLRRDLNADVLQRVLNPLRWFTPEGRTAAAATLYDT
                     TIFKGATFADLQRRGGPLIVINATDLEAGTRFAFLQEHFDLLCSDLRGFPLAQAVAAS
                     SAVPVLMEPVVLENHAGCAPPALPQPPADSPQLQQVVDGLRSYADKQARRYVHLADGG
                     LTDNLGLRAFQETIDLAGGLRAFLRKIDRAPNGHLVMISVDASGNAAAGLGLDRRAPS
                     MLQAIDVMSDVQLHRYNAATLEQTQASLQRWARGVSTPEQAVQPWLVTLTLRDIADPA
                     LRQRLAKVPTGFSLEPAQADELIAAGRALLRDNPDFRRLLEALGGTGAASSTK"
     gene            1537..3090
                     /gene="rmuC"
                     /locus_tag="PK818_07235"
     CDS             1537..3090
                     /gene="rmuC"
                     /locus_tag="PK818_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HQY82559.1"
                     /translation="MESNTLLLLVLVAALVAIGLLLALLLRKPDSRMEHALREERDRV
                     ASQLQAQRDDLQAKAAETARLGERIAGLERSDAELAEAKDALRAEQLAAGDLRVQLAS
                     RNELLQQAESRLGKAEAELAQLRREFDALRQLHADAVANLSHSERTNSEMKDFLANAQ
                     ERLSGVFAELAGKTFDERTQKAAQQSKGDLEMLLKPFAEQLSGFRNRVDTLYGEEAKE
                     RAALAGKVDELKTLNQGMAERANELTRALRGSSKVRGDWGELMLESVLQGSGLVEGAH
                     YERQKSSNDDEGQRLQPDIVVRLPDDRRIVVDSKVNLVDWQDAMNAASPEEQQEAMRR
                     HAIALRQHVKDLGEKNYPKAVGEGALEITIAFVPIEGALSAALGFDGALQTYAFDRKI
                     VFASPNTLMALLRVVERLWTRDKIQREAGEIAKVGGLVLDSLQNFLADFDSVGKQLDQ
                     ARTAFNAARGKLTESNQALIPRARRLHQLGAKGKKALSAELIGDAEPLASLASETPAA
                     RVDEPDDDA"
     gene            3142..6495
                     /gene="recC"
                     /locus_tag="PK818_07240"
     CDS             3142..6495
                     /gene="recC"
                     /locus_tag="PK818_07240"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HQY82560.1"
                     /translation="MTPPVHDFRLYHSNALDVLAELLAEELRKDVPGRPLLEPDTVLI
                     PQAAMRRWLQATLARRHGIAANLAFLTPGEFVARALDANLGKAAGDLDADSLHWRLYA
                     ALNDPALLAKPAMAQLAAYLAGDDPLKPWALAGELAGVFEKYQAWRRDWLLRWESGAE
                     ADDPQAILWRAVAAGRSTRARRIQAYLTRFAEGDALPAGVPARLFAFGTLNVSPDVLR
                     VMATQARIGTLHFYLPTPSREYWGDLQALGARLREGRDPFDADAGENPLLRDWGAAGK
                     DFMALLGSYEVVHPSGEIAAYADPADVEGTGLLQRMQADLFHRRAAPSGSKREAVETG
                     DASLQFHACHTRLRELQVLHDRLRALLEDPRFDPPLQPREIAVLAPDIDPYAPYLDAV
                     FGGTPGQAIPYAIADASPLAGEPLAEVFLRLLALPVARFGLEELLDLLASPPVAEAAG
                     LEAADFERLHGWLHAAGARWGIDAAHRARRGAPADDAYTWQFALDRLLLGHASGSDDD
                     IDGVAPWPDLEGGALDALDTLLRLLRVLARHQRLLGEALPPAQWRERLLGLLDALLPK
                     PPASADGQRALDRLRTLIDAFAKQAGKADFETPVPVEVVRAHFTARLTEADTRAPLLT
                     GGVSFARMVPMRLLPFRVVCLLGMDDGAFPRRDPAAGLNRLTAELGSDRRRHGDRSTR
                     DDDRYLFLQLFTAAQDVFYVSWLGADARDGSAREPSALVSELLAAAADYHAKPVEAAR
                     ELVLRHPLQPFSPAAFGVLNQDGKQDPRTFSYRGHWHPAAGRLSGARQPLRPWMDAAH
                     ALSAPAEAETALSLDALRRFLMAPAEQFLRQRLGLRLPEVEAAGEDIEPLQAPRAGLQ
                     RTLLQHAVFGGLLAGQGTSALHASLRARGLLPSGPLGARVLAEIEGEVAPYARAFANW
                     RNGAETTSLLLEAEVDGVRVHGRLGEAYPDGIVRLRFGKPSGPSSLRNGLDWLLASAA
                     GVDLPFVEFHEAPDAGIGPHLRPPLSRDSAIDALHALVALRRDGLRRPLPFAPYSAWE
                     LFTATDAARGVNEAASKWRGREGGWAERDSEALRLALRGRDPFADAAALEEFAGLAFV
                     IFGAVTRGEPMPAPIINVEALPDDAEDEA"
     gene            6492..>7967
                     /locus_tag="PK818_07245"
     CDS             6492..>7967
                     /locus_tag="PK818_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017112939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQY82561.1"
                     /translation="MSTVRDPYLELPLDGVRLVEASAGTGKTYTLATLVTRLVVERGL
                     RIGQILAVTFTDAATQELRKRIRERLQLTLALIDAPLADAEGSEATLTRQLLQSHRER
                     SEESDDALRHRLRQATLDIDLAAIFTIHGFCARVLREHALESGHGFDPPELLANDRDL
                     RAELAADLWRQHAQDAATADDLGALWTNHEALAEDLRVLLREPVLLPADAPLPPDPKP
                     ALLVAGAGLADAYRQHGEAFFADLLAALDGKVLNGNSYRRDWVEALCRQWPAWCEAGD
                     FAAPLDARIARLTTEALQAKANKGKDALVPKSPMSAEISTYLEAMQAQAEYVARRRIA
                     LLHRIRDHARARLAQLKQQHRVQTYDDLIDGVADALDGEKGDALARQLRSQYAVALVD
                     EFQDTDARQWAIFRRVFGSVPSEGDGNLDEPASGHSPPALESARTGGSQARFLALIGD
                     PKQAIYSFRGGDVHTYLDAGTHAVAAPPLSHNFRSRPAVLQA"
BASE COUNT         1187 a         2820 c         2792 g         1168 t
ORIGIN      
        1 tgcgccgcgc cgcgttctac gccggccgca ccgtgcaggt gctgaaggtc gccggtgccg
       61 gccccgacca cccgtggctg gcgcaggtgc cggagtcgcg ctacctgaag gcggtgttct
      121 gccgggtgct ggattgagcg cgccacgatg acccgcctgc gcccgctcgc gctgctcctg
      181 ctgccgctgc tggtcggctg cgcgacctac gccaccatcg gcaaccagcc gctgacctcg
      241 gccgccgcgg ccgccccggg ctacgacatg gggcaggtcg gggtcgaggg ccggcgcgac
      301 gacgtcaccc tcgcgctggc gttctccggc ggcggctcgc gggcggcggc gcttgcttac
      361 ggcgtgctgc tggaactgcg cgagacccgg gtcggggcag cgcgcgagca ccggctgctg
      421 gacgaggtgg acgcgatcag cgcggtgtcc ggcggcagct tcaccgctgc ctactacggc
      481 ttgcacgatg aggcgacctt ccgcgacttc gagccggggt tcctgcgtcg cgacctcaat
      541 gccgacgtcc tgcagcgggt cctcaacccg ctgcggtggt tcactcccga gggccgcacc
      601 gcggccgccg cgaccctgta cgacaccacc atcttcaagg gcgcgacctt cgccgacctg
      661 cagcgccgtg gcgggccgct gatcgtgatc aacgccaccg acctcgaggc cggcacccgc
      721 ttcgccttcc tgcaggagca tttcgacctg ctgtgctcgg acctgcgcgg gtttccgctg
      781 gcgcaggcgg tggctgcgtc ctccgcggtg ccggtgctga tggagcctgt ggtgctggag
      841 aaccatgccg gttgcgcgcc gccggcgttg ccgcagccgc ctgcggattc gccgcagttg
      901 cagcaggtgg tcgatggcct gcgcagctat gccgacaagc aggcacgtcg ctacgtccac
      961 ctcgctgatg gcggcctgac cgacaacctc ggcctgcgtg cgttccagga aaccatcgac
     1021 ctcgctggcg gcctgcgcgc gttcctgcgc aagatcgacc gggcgccgaa cgggcacctg
     1081 gtgatgatct cggtcgacgc ctccggcaac gccgccgccg ggctcgggct ggatcgccgc
     1141 gcaccgtcga tgctgcaggc gatcgacgtg atgagcgacg tccagctgca tcgatacaac
     1201 gccgcgaccc tcgagcagac ccaggccagc ctgcagcgct gggcgcgcgg ggtgtccacc
     1261 ccggagcagg cggtgcagcc gtggctggtg accctgaccc tgcgcgacat cgccgatccg
     1321 gccctgcgcc aacgcctggc caaggtgccg accggtttca gcctcgaacc tgcgcaggcg
     1381 gacgagctga tcgccgccgg ccgtgcgctg ctgcgcgaca acccggactt tcggcgcctg
     1441 ctggaggcgc tgggagggac cggcgccgca tcttcgacca agtagtcggc ggcggccgtt
     1501 cgcagtccct gcgaccgcca tcgctacact ggcgggatgg aatcgaacac cttgttgctc
     1561 ctcgtgctgg tcgccgcgct ggtggcgatc gggctgttgc tcgccctgct gctgcgcaaa
     1621 cccgacagcc gcatggagca cgcgctgcgc gaggagcggg atcgcgttgc ttcgcagctc
     1681 caggcccaac gcgacgacct gcaggcgaaa gccgcggaga cggcgcgcct gggcgagcgc
     1741 atcgccggtc ttgagcgcag cgatgcggag ctggccgaag cgaaggacgc gctgcgcgcg
     1801 gagcagctgg ccgcgggcga tttgcgcgtg cagctggcct cgcgcaacga gttgttgcag
     1861 caggccgagt cgcgcttggg caaggccgag gcggagcttg cgcagctacg ccgcgagttc
     1921 gatgcgcttc ggcagttgca tgcggacgca gtggccaacc tcagccacag cgagcgtacc
     1981 aacagcgaaa tgaaggactt cctggccaac gcacaggaac gcctgtccgg cgtgttcgcg
     2041 gagttggcgg gcaagacctt cgacgaacgc acgcagaagg cggcgcagca atccaaggga
     2101 gacctggaaa tgctgctgaa gccgttcgcc gagcagttgt ccggcttccg caatcgtgtc
     2161 gataccctgt atggcgagga agcgaaggag cgcgccgccc tcgccggcaa ggtggacgag
     2221 ctgaagaccc tcaaccaggg catggccgaa cgcgccaacg agcttacccg cgccctgcgc
     2281 ggcagctcga aggtgcgcgg cgactggggc gaattgatgc tggaaagcgt gctgcagggc
     2341 tccggcctgg tggaaggcgc gcactacgaa cggcagaaat ccagcaacga cgacgagggc
     2401 cagcgcctgc agccggacat cgtggtgcgc ctgccggacg accgccgcat cgtggtcgac
     2461 agcaaggtga acctggtcga ctggcaggac gcgatgaacg ccgccagccc cgaggagcag
     2521 caggaagcga tgcgacggca tgccatcgcg ttgcgccagc acgtcaagga cctgggcgag
     2581 aagaattacc cgaaggcggt cggcgaaggt gcgctggaaa tcaccatcgc cttcgtgccg
     2641 atcgaaggcg cgctgtcggc ggcgctgggc ttcgatggcg cgctgcagac ctacgccttc
     2701 gacaggaaga tcgtgttcgc ctcgcccaac acgttgatgg cgttgttgcg cgtggtcgag
     2761 cgcctgtgga cgcgcgacaa gatccagcgc gaagcggggg agatcgccaa ggtgggcggc
     2821 ctggtgctcg attcgctgca gaacttcctc gccgatttcg acagcgtcgg caagcagctg
     2881 gaccaggcgc gcaccgcgtt caacgccgcg cgcggcaagc tcaccgaatc caaccaggcg
     2941 ctcatcccgc gcgcgcgccg cctccaccag ctcggtgcca agggcaagaa ggcgttgtcg
     3001 gcggaattga tcggcgatgc cgaaccgttg gcgagcctgg ccagcgaaac acctgctgcc
     3061 cgcgtcgacg agcccgacga cgacgcctga ccggcgccgt cgcgttgcgt gcgacacggc
     3121 tccggcacaa tccctcggac gatgaccccg cccgtgcacg acttccgcct ctaccactcc
     3181 aacgcgctcg acgtgctggc ggagctgctg gccgaggaac tgcgcaagga cgtgcccggc
     3241 cggccgctgc tggagccgga cacggtgctg atcccgcagg cggcgatgcg ccgctggctg
     3301 caggccacgc tggcgcgccg gcacgggatc gccgccaacc tcgcgttcct gacccccggc
     3361 gaattcgtcg cgcgcgcgct ggacgcgaac cttggcaagg ccgccggcga cctcgatgcc
     3421 gacagcctgc actggcgcct gtacgcggca ttgaacgatc cggcgctgct ggcgaagccg
     3481 gcgatggcgc aactggcggc ctacctggcc ggcgacgatc cgctcaagcc atgggcattg
     3541 gccggcgagc tggccggcgt gttcgagaaa taccaggcct ggcgccgcga ctggctgctg
     3601 cgctgggaat ccggcgccga agccgatgat ccgcaggcga tcctgtggcg cgcggtcgcg
     3661 gccggccgca gcacgcgggc acggcgcatc caggcctacc tcacgcgctt cgcggagggc
     3721 gatgctttgc cggccggcgt gcccgcgcgg ctgttcgcgt tcggcaccct caacgtctcg
     3781 cccgacgtgt tgcgggtgat ggcgacccag gcgcgcatcg gcaccctgca tttctacctg
     3841 cccacgccga gccgcgaata ctggggcgac ctgcaggcac tgggcgcacg cctgcgcgag
     3901 ggccgcgacc cgttcgacgc cgatgccggc gagaacccgc tgctgcgcga ctggggcgcg
     3961 gccggcaagg acttcatggc cctgctcggc agctacgagg tcgtgcatcc atcgggtgag
     4021 atcgcggcct atgccgatcc cgcggacgtc gagggaacgg gcctgctgca acgcatgcag
     4081 gccgacctgt tccaccgccg cgccgcgcct tccggcagca agcgcgaggc cgtggaaacc
     4141 ggcgatgcga gcctgcagtt ccacgcctgc cacacccgcc tgcgcgaact gcaggtgctg
     4201 cacgaccgcc tgcgcgcgct gctcgaagac ccgcgcttcg acccgccgct gcagccgcgc
     4261 gagatcgcgg tgctggcgcc ggacatcgat ccgtatgcgc cgtacctgga cgcggtgttc
     4321 ggcggcacgc ccggccaggc gattccctac gcgatcgccg atgccagccc gctcgccggc
     4381 gaaccgctgg ccgaggtgtt cctgcgcctg cttgcactgc cggtggcgcg cttcgggctg
     4441 gaggaactgc tggacctgct cgccagtccg ccggtggccg aggctgcggg cctggaagcg
     4501 gcggatttcg aacgcctgca cggttggctg catgcggccg gtgcgcgctg gggcatcgac
     4561 gccgcgcatc gcgcgcgccg cggcgcgccg gcggacgatg cctacacctg gcagttcgcg
     4621 ctggatcgcc tgctgctggg ccatgccagt ggcagcgatg acgatatcga tggcgtcgcg
     4681 ccctggccgg acctggaagg cggcgcgctc gacgcgctgg atacattgct gcgcctgttg
     4741 cgggtcctgg cacgccacca gcgcctgctc ggcgaggcac tgccgcccgc gcagtggcgc
     4801 gaacgcctgc tcggcctgct cgacgcactg ttgccgaagc cgcccgcgtc ggcggacggg
     4861 caacgcgcgc tggatcgttt gcgcacgctg atcgatgcct tcgcgaagca ggcaggaaaa
     4921 gcggatttcg aaacgccggt tccagtcgag gtggtgcgtg cccacttcac cgcgcgcctg
     4981 accgaggccg atacccgcgc accgctgctc accggcggcg tcagcttcgc gcggatggtg
     5041 ccgatgcggt tgctgccgtt ccgggtggtc tgcctgctcg ggatggacga tggcgcgttc
     5101 ccgcggcgcg atccggcggc cggcctcaac cgcctcaccg ccgagctggg cagcgacagg
     5161 cgccggcacg gcgaccgctc tacccgcgac gacgaccgct atctgttcct gcaactgttc
     5221 accgccgcgc aggacgtgtt ctacgtgagc tggctcggtg ccgatgcgcg cgacggcagc
     5281 gcgcgcgaac cgtccgcgct ggtatccgaa ctgctcgccg cggcagcgga ctaccacgcg
     5341 aagcccgtcg aggccgcacg cgagctggtg ttgcggcatc cgctgcagcc gttctcgccg
     5401 gccgcgttcg gcgtcctgaa ccaggacggc aagcaagatc cgcgcacctt cagctatcgt
     5461 ggccattggc atcctgccgc tggccgtttg tccggcgcac gccagccgct caggccctgg
     5521 atggatgcgg cgcacgcgct gtccgccccg gccgaggccg agacggcgtt gtcgctggat
     5581 gcattgcgcc gtttcctgat ggcaccggcc gagcagttcc tgcgccagcg cctgggcctg
     5641 cgcctgccgg aggtggaagc cgcgggcgaa gacatcgagc cattgcaggc accgcgcgcc
     5701 ggcctgcaac ggaccctgct gcagcacgcg gtgttcggtg gcctgctcgc ggggcaaggc
     5761 accagtgccc tgcacgccag cctgcgcgcg cgcggcctgt tgccctccgg gccgctgggc
     5821 gcacgcgtgc tggcggagat cgaaggcgaa gtcgcgccct atgcgcgcgc cttcgcgaac
     5881 tggcgcaacg gtgcggaaac gacatcgctg ctgctggaag cggaggtcga tggcgtgcgc
     5941 gtgcatggcc gtctgggcga ggcctacccg gatggcatcg tgcgtttgcg cttcggcaag
     6001 ccgagcggtc cgtcgtcgct ccgcaacgga ctcgactggc tgctggcgtc cgccgcgggc
     6061 gtggaccttc ccttcgtcga attccatgag gccccggacg ccggcatcgg cccgcatctg
     6121 cggccgccgc tgtcgcggga ctccgcgatc gatgcgctgc atgcgctggt tgcgctgcgt
     6181 cgtgacggcc tgcgccggcc gttgccattc gcgccctaca gcgcgtggga gcttttcacc
     6241 gcgacggatg cggcgcgcgg cgtgaacgaa gcggcaagca agtggcgggg tcgcgaaggt
     6301 ggatgggcgg aacgtgacag cgaagccctg cgactggccc tgcgcggccg cgatccgttc
     6361 gccgatgcgg cggcgttgga ggaattcgcg gggctcgcct tcgtcatctt cggcgcggta
     6421 acgcgcggcg agccgatgcc ggctccgatc atcaatgtcg aagccttgcc ggacgacgcg
     6481 gaggacgagg catgagcacc gtccgcgacc cgtacctcga gctgccgctg gatggcgtgc
     6541 gcctggtcga ggccagcgcc ggcaccggca agacctacac cctcgccacc ctggtcaccc
     6601 gcctggtagt cgaacgcggg ctgcgcatcg gccagatcct cgcggtcacc ttcaccgatg
     6661 ccgccaccca ggaactgcgc aagcgcatcc gcgagcggtt gcagctcacc ctggcgctga
     6721 tcgacgcgcc gctcgccgac gctgaaggca gcgaagccac gctgacccgg caactgctgc
     6781 aatcgcaccg cgaacgcagc gaagaatccg acgacgccct gcgccatcgc ctgcgccagg
     6841 ccacgctgga catcgacctc gccgcgatct tcaccatcca cggcttctgc gcacgcgtgc
     6901 tgcgcgaaca cgcgctggaa tcgggccacg gcttcgatcc gcccgaactg ctggcgaacg
     6961 accgcgactt gcgcgcggaa ctcgccgcgg acctctggcg ccagcatgca caggacgcgg
     7021 ccacggccga tgatcttggc gcgctgtgga cgaaccacga agcactcgcc gaagatttgc
     7081 gcgtgctcct gcgcgagccg gtgctgttgc cggcggatgc gccgctgccg ccggatccga
     7141 agccggcact gctggtggcc ggcgccggac tggcggacgc gtaccgacag cacggcgaag
     7201 cattcttcgc ggacctgttg gccgcgttgg acggcaaggt gctgaacggc aacagctatc
     7261 gcagggactg ggtcgaggcg ctgtgccggc agtggccggc gtggtgcgaa gccggcgatt
     7321 tcgccgcgcc gctggacgcg cgcatcgcgc ggctcaccac cgaggcgctg caggccaagg
     7381 ccaacaaggg caaggacgcg ctggtgccga agtcgccgat gagcgcggag atttccacgt
     7441 atctcgaggc catgcaggcg caggccgaat acgtcgcgcg ccgccgcatc gccctgctgc
     7501 accgcatccg cgaccacgcc cgcgcgcgcc tggcccaact caagcagcag caccgcgtgc
     7561 agacctacga cgacctgatc gatggcgtcg ccgatgcact cgatggcgag aagggcgatg
     7621 cgctcgcacg ccaactgcgt tcgcaatacg cggtcgccct ggtcgacgaa ttccaggaca
     7681 ccgatgcgcg ccagtgggcg atcttccgcc gcgtgttcgg atctgttcca tcggaagggg
     7741 acggcaatct tgacgaaccg gccagcgggc atagcccgcc ggcgctcgaa agcgcgcgaa
     7801 ccggtggttc gcaagcgcgc tttctcgccc tgatcggcga tcccaagcag gcgatctaca
     7861 gctttcgcgg cggcgacgtg cacacgtatc tcgatgccgg cacgcacgcc gtcgccgcgc
     7921 cgccgctttc gcacaacttc cgctcgcgcc cggcggtgct gcaggcg
//
LOCUS       DAORUK010000093         7948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_10160_length_7948_cov_19.065881, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000093 DAORUK010000000
VERSION     DAORUK010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10160_length_7948_cov_19.065881"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..919
                     /locus_tag="PK818_07250"
     CDS             176..919
                     /locus_tag="PK818_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82562.1"
                     /translation="MNRIMVHAAASLLVLALGSVHASGINSINGGMPNRISMNVTVPK
                     QTQGATFGEKVNSGLQASGRSAAQGASLSIVIECGQVACAFVFPDGSGHRADLQRRRI
                     EALASAQGAALRRGSPGPAMLGGALPGGAVISAAVSSVGNLAGSGGGAAAAAYAATGR
                     ASDGGKEGLATASAVPGVIDVVEPLADGDYALTLVVEADTSGAKGAPRTQVRTVSPPR
                     VEITVVFVLAGGAMRARHDTAMNTIRNMK"
     gene            complement(935..1582)
                     /gene="rpiA"
                     /locus_tag="PK818_07255"
     CDS             complement(935..1582)
                     /gene="rpiA"
                     /locus_tag="PK818_07255"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HQY82563.1"
                     /translation="MSEMKKLAAEKAIDYVEDGMIVGVGTGSTVAYFIDALAAWKDRI
                     AGAVSSSEQSTARLKSHGIEVIDLNAAGPLSLYVDGADECDPHRRLIKGGGAALTREK
                     IIAEASKTFVCIIDPGKQVSVLGRFPLPVEVIPMARSLVARELVRLSGGQPVWRDGVV
                     TDNGNVILDLHNLSIVDPVGLEREINQIPGVVSVGLFARRPADVVIVGGEPPLVL"
     gene            complement(1665..2135)
                     /locus_tag="PK818_07260"
     CDS             complement(1665..2135)
                     /locus_tag="PK818_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HQY82564.1"
                     /translation="MSRPKRYWMMKSEPDAFSIDDLERVGTEPWSGVRNYQARNFMRD
                     GMRPGDGVMIYHSNCKVPGLAGLARVASIAYPDETQFDRGSHYFDQKATREQPRWFLV
                     DVAFDRKLTRVIPLQEIKRHTDELGEGFALTARGSRLSVFPVTAAQWKLLLSLE"
     gene            complement(2132..2734)
                     /locus_tag="PK818_07265"
     CDS             complement(2132..2734)
                     /locus_tag="PK818_07265"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HQY82565.1"
                     /translation="MNPEDRSTLRRELRERRRALPATTRITAADSLADRLLALPFFDG
                     MPVANKAWVAGYWAMDGEIGLHAFQMRLPAALAYCLPILHGDSLRFAPWAPGDALVTN
                     RYGIPEPDVAADCALEPRDMALVVLPLVGFDASGHRLGMGGGWYDRSFAFRHAQEPPP
                     LLVGAAFDVQCVPALVAQAWDVRLDAVCSERATYLAGDRT"
     gene            complement(2755..2939)
                     /gene="ssrS"
                     /locus_tag="PK818_07270"
     ncRNA           complement(2755..2939)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK818_07270"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3015..3326)
                     /locus_tag="PK818_07275"
     CDS             complement(3015..3326)
                     /locus_tag="PK818_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HQY82566.1"
                     /translation="MSGNEPGKSEPVTVRILDREYTVGVSDGERASLTSAARLLDQRM
                     RELRGSNKLVAPDRLAVLTALNLAHELQQLREERGGNDAVFRLLDAANARLDGLLGPA
                     A"
     gene            complement(3323..3544)
                     /locus_tag="PK818_07280"
     CDS             complement(3323..3544)
                     /locus_tag="PK818_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HQY82567.1"
                     /translation="MDSRDLAAELQRLLARIDQLAALLQRTQDENRSLRQQHEQMASE
                     RAQLLAKQEQARTRVEAMISRLKSLEQHT"
     gene            complement(3577..5802)
                     /locus_tag="PK818_07285"
     CDS             complement(3577..5802)
                     /locus_tag="PK818_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQY82568.1"
                     /translation="MPATHYMALLLALLVLALAGVVLGGHRRREREQREHLRALRERE
                     EKLRLALWASSEYYWQYDLERRELESIRIEPGRKDDLNLRIDLDADHQIHPDDLPLVL
                     DRLRAYVRGDAPMFLSEHRVQDGTDGWRWMRARGRAVSRNDAGRITRIAGTARDVSAQ
                     REHERERRIATEVMHNMAEAVVVIDDEFRYVMVNPAFTRMSGYELAEVVGNDASLTNS
                     DQHDAAFYAQSRAAMLEQGHWAGEMWQRRKDGREFLCAMQSNAINEPGSPHRLFVLVA
                     TDITDRRRIEQELRYLANYDTLTNLPNRTLLAERLSRAIVRARRQGSCVALLFLDLDR
                     FKDINDSLGHATGDRILRMAAQRLQRAVGPSHTVARIGGDEFTVVLEDLAHAGEADTC
                     AQRVIDAFDEPLLIDERHEIAITPSIGISLYPDHAQVPTDLLKHADTAMYQAKAAGRH
                     TFLRYTESMDSSTRQRAGLIASLRRAIERNELRLVYQPQLSLADGGIRCVEALLRWYS
                     TEHGEVSPVQFIPLAEESGMIVGIGEWVLREACNTLADWQRAGLPGVTMAVNVSATQL
                     LHSDLPRAVERILRETGIDPGQLELELTESVLMAKAGLAAERLQFFRRIGVSIAVDDF
                     GTGYSSLAYLRRLPITTLKIDKAFIDDLGRPGDNEDAAITTTIIAMARSLGLGVVAEG
                     VETAEQLAFLRQHRCDMVQGYWLSKPLPAEQCLAFMLEWPGLPGLAPRATAADARHPA
                     P"
     gene            5893..6435
                     /locus_tag="PK818_07290"
     CDS             5893..6435
                     /locus_tag="PK818_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HQY82569.1"
                     /translation="MSETELPGWADVAAAADRLDLASTPAELHGGLCGWLAAGGDDAP
                     SWLALVLADPSLPVPDRGGVLDRLRTASAAQLCNADFGFGLLLPDDDDVAERAGAMFA
                     WCRAFLGGFGLAVGGKKLSEDGEEALGDIANLAAARVDDVDPEGDEESLTEIEEYLRM
                     AVLLLHADCTLAPRQRQRLH"
     gene            6436..7764
                     /gene="pepP"
                     /locus_tag="PK818_07295"
     CDS             6436..7764
                     /gene="pepP"
                     /locus_tag="PK818_07295"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007967663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HQY82570.1"
                     /translation="MRCTSSERKALARRRKALMQAAGDGAILVLPAAPERIRSRDTHY
                     PYRQDSDFWYLTGCNEPEAVLVLVPGRRHGESILFCRERDPEREGWDGPRLGPEGAVD
                     ALGMDDAYPISDIDEILPGLLEGRRRVHYHLGRDAEFDLKLIGWINRVRAQSRQGAQP
                     PQEFLALGHLLDEMRLFKSADEVKLMQRAADISVLAHRAAMRAARPGIHEYELQAELE
                     RVFRMHDAQPAYASIVGAGANACVLHYRDNAARSRDGDLVLVDAGAEYRGYAADITRT
                     FPLSGRFTREQRALHDLVLAAQAAALAQARPGVPYEAGHEAAVETLTEGLLSLGLLKG
                     RLEAAIAAGDYKRFYRHKTGHWLGLDVHDVGEYRIDGESRLLEPGMVFTIEPGLYIPE
                     DDVGVPAKWRGIGIRIEDDVLVTADGHRVLTDALERSADEIETLMAAAKP"
BASE COUNT         1215 a         2612 c         2833 g         1288 t
ORIGIN      
        1 tttttctttg cgccacgcac cagccaaaat agcgtgcgcc ggggcgacca tgaggatcgg
       61 tggcgaaatc gtgcaaggtc cagcgaccaa cggttccctc gggctgtcgc ccggcaggat
      121 gatccaccgg tatcagcgta ggattccggt atcagcggcc atccacggaa gcgacatgaa
      181 caggatcatg gtccatgcgg ctgcaagcct gttggtcctg gcgctgggat cagtgcatgc
      241 cagtggcatc aacagcatca acggggggat gccgaaccgg atttccatga acgtgaccgt
      301 gcccaagcaa acccagggtg cgacattcgg tgagaaggtg aattccgggt tgcaagcttc
      361 gggccgatcg gccgcgcagg gggcatcgct gtcgatcgtg atcgagtgcg gtcaggtggc
      421 ttgcgcgttc gtgttccctg acggttccgg ccaccgcgct gacctgcagc gtcggcggat
      481 cgaggccctt gcgtcggcgc agggtgccgc gctgcgccga ggaagcccgg gcccggcgat
      541 gctgggcggt gcgctgccag gcggtgcggt catatcagcc gcggtgtcat cggtgggcaa
      601 cctggccggt agtggcggcg gcgctgcggc ggcagcttat gcggcaaccg gccgggcatc
      661 ggatggtgga aaggagggct tggcaaccgc gagcgcagtc cccggcgtga tcgacgtggt
      721 cgagccgctc gccgatggtg actacgcgtt gacgcttgtc gttgaggcgg acacgagtgg
      781 tgcgaagggg gcgccgagga cccaggtgcg taccgtgtcg ccaccgcggg tcgaaatcac
      841 ggtcgtgttc gtgttggctg gtggcgccat gagggcacgc cacgacactg caatgaacac
      901 gatccgcaac atgaagtagg cgcgcaggcc tgcttcagag caccaggggc ggctcgccgc
      961 ctacgatcac cacgtcggcg gggcgtcgcg cgaacaggcc gacgctgacc acgcccggga
     1021 tctggttgat ttcgcgttcc agtccgacgg gatcgacgat cgaaaggttg tgcaagtcga
     1081 ggatcacgtt gccgttgtcg gtcaccacgc cgtcgcgcca gaccggctgc ccgccgctga
     1141 gacggaccag ttcgcgggcc accaggctgc gtgccatcgg gatcacctcg accggcagcg
     1201 ggaaccggcc gagcaccgac acctgctttc ccgggtcgat gatgcacacg aacgtcttgc
     1261 tggcctcggc gatgatcttc tcgcgggtca atgccgcgcc gccacccttg atcaggcgcc
     1321 tgtgcggatc gcattcgtcg gcaccgtcca cgtacagcga cagcgggccg gcggcattga
     1381 ggtcgatcac ctcgatcccg tgcgatttca gccgagcggt gctttgctcc gagctggata
     1441 ccgcgccggc gatccggtcc ttccatgcgg ccagcgcgtc gatgaaatag gcgaccgtgg
     1501 acccggtgcc gacacccacg atcatgccgt cctcgacgta gtcgatggcc ttttcggcgg
     1561 cgagtttctt catttcgctc attgcggatt cctgttctgg tcgggcagcg gcgcgagccg
     1621 cgacgcacgg gtgcggggct gcggccgaag ccgctcctgc agggtcattc gagggagagc
     1681 agcagtttcc actgggcggc cgtgaccggg aacaccgaga gccggctgcc cctggcggtc
     1741 agcgcgaagc cttcgccgag ttcatcggta tgacgcttga tctcctggag cgggatcacg
     1801 cgcgtgagct tgcgatcgaa cgccacgtcg accaggaacc atcgcggctg ttcgcgggtc
     1861 gccttttggt cgaagtagtg ggacccacga tcgaattggg tctcgtccgg ataggcgatg
     1921 ctggccaccc gtgccaggcc ggcgaggccc ggcaccttgc aattggagtg gtagatcatc
     1981 acgccgtcgc cggggcgcat gccgtcgcgc atgaagttgc gcgcctggta gttgcgcacg
     2041 ccgctccatg gctcggtgcc gacgcgctcg aggtcgtcga tggaaaacgc gtccggctcg
     2101 gacttcatca tccagtagcg cttcggcctg ctcatgtgcg atccccggcg aggtaggtgg
     2161 cgcgctcgct gcacaccgcg tcaaggcgca cgtcccaggc ctgggcaacg agcgcaggca
     2221 cgcattggac gtcgaaggcg gcacccacca gcaagggcgg cggttcctgg gcatgccgga
     2281 acgcgaagct gcgatcgtac cagcccccgc ccatgccaag ccggtggccg cttgcgtcga
     2341 agcccaccag cggcagcacg accagcgcca tgtcccgggg ctcgagcgcg cagtcggcgg
     2401 cgacatccgg ttccgggatg ccgtagcgat tggtgaccag cgcatcgccc ggtgcccatg
     2461 gcgcgaagcg caagctgtcg ccatgcagga tgggcaggca atacgcaagc gcagcgggca
     2521 ggcgcatctg gaacgcgtgc aggccgatct cgccgtccat cgcccagtag ccggcgaccc
     2581 aggccttgtt ggcaaccggc atgccgtcga agaacggcag cgcgagcagg cggtcggcca
     2641 gcgaatccgc ggcggtgatg cgggtcgttg cgggcagtgc gcgtcggcgc tcgcgcagtt
     2701 cacgccgcag cgtggatcga tcttctggat tcacgcgcgg cagtcggaag gaagaaggtc
     2761 tccgccgtgg cgatgcgtgc gaaacgacct tgaacccggg gttcaagtgg gaacgcgtgg
     2821 cggccttcgg gcttcccgct cagggcggac ttgcacgcct ggtcgccaat gcatccccgt
     2881 ggtcgtaatt aagggacaag gcgaatgttt gcacacgccg tcgcacacag cagaggaccg
     2941 gcccagtata acgcgcgatg caacgagtcg atggccgctg gtcggatcac gccgtgcggc
     3001 gaacgcggcg cgattcaggc ggcgggtccg agcaacccat ccagccttgc attggcggcg
     3061 tcgagcagcc tgaacacggc atcattgccg ccgcgctcct cgcgcagttg ctgcagttcg
     3121 tgcgccaggt tgagcgcggt cagcaccgcc agccgatccg gtgcgaccag cttgttgctt
     3181 ccgcgcaact cgcgcatgcg ctggtcgagc aggcgcgcgg ccgaggtgag gctggcgcgt
     3241 tcaccgtcgc tgaccccgac cgtgtactcg cggtcgagga tgcggacggt gaccggctcg
     3301 ctcttgcctg gttcgttgcc gctcacgtgt gctgctccag cgacttaagg cggctgatca
     3361 tcgcttccac gcgggtccgg gcctgctcct gcttcgccag caactgtgca cgttcgctcg
     3421 ccatctgctc gtgctgctgg cgcaggctcc ggttctcgtc ctgggtgcgc tgcagcagtg
     3481 ccgcaagctg gtcgatgcgc gccagcaggc gttgcagttc ggcggccagg tcgcgggagt
     3541 ccatgccggc atgatgggcg cagcagcgcg gcagggtcaa ggcgcggggt ggcgggcatc
     3601 cgctgcggtg gcacgcgggg cgagccccgg caggcccggc cattcgagca tgaaggccag
     3661 gcactgctcc gccggcagcg gtttggacag ccagtagccc tgcaccatgt cgcatcggtg
     3721 ctggcgcagg aaggcgagct gctcggccgt ctccacgccc tcggcgacga cgcccaggcc
     3781 cagcgagcgc gccatcgcga tgatggtggt cgtgatcgcc gcgtcttcgt tgtcgccggg
     3841 gcggccgagg tcgtcgatga acgccttgtc gatcttgagg gtggtgatcg gcaggcggcg
     3901 caggtaggcc agcgaagagt agccggtacc gaagtcgtcg accgcgatcg acacgccgat
     3961 gcggcggaag aactgcaggc gctcggcggc aagcccggcc ttcgccatca gcacgctctc
     4021 ggtcagttcg agctcgagct ggccgggatc gatcccggtt tcgcgcagga tgcgttccac
     4081 cgcccgtggc aggtcgctgt gcagcaactg cgtcgccgac acgttcaccg ccatggtcac
     4141 ccctggcagg ccggcgcgct gccagtccgc gagggtgttg caggcttcgc gaagcaccca
     4201 ttcgccgatg ccgacgatca tcccgctttc ctccgcgagc gggatgaact ggaccgggga
     4261 gacctcgccg tgctccgtgc tgtaccagcg cagcagggcc tcgacgcagc ggatgccgcc
     4321 atccgccagc gacagttgcg gctggtacac caggcgcagt tcgttgcgtt cgatcgcccg
     4381 ccgcagcgag gcgatcaacc cggcgcgctg gcgggtgctg ctgtccatcg actcggtgta
     4441 gcgcaggaac gtgtggcggc ccgccgcctt ggcctggtac atcgcggtgt cggcatgctt
     4501 gagcaggtcg gtcggcacct gcgcgtggtc gggatagagg ctgatgccga tcgagggcgt
     4561 gatcgcgatc tcgtggcgct cgtcgatcag cagcggctcg tcgaaggcgt cgatcacccg
     4621 ctgtgcgcag gtgtctgcct cgccggcatg cgccaggtcc tccagcacca cggtgaattc
     4681 gtcgccgccg atgcgcgcga ccgtgtgcga ggggcccacc gcgcgttgca ggcgctgcgc
     4741 cgccatccgc aggatgcggt cgccggtggc gtggccaagc gagtcgttga tgtccttgaa
     4801 gcggtcgagg tcgaggaaca gcagggccac gcaactgccc tggcgccgcg cgcgcacgat
     4861 cgcccgcgac aaccgctccg ccagcagggt gcggttcggc aggttggtca gggtgtcgta
     4921 gttggcgagg tagcgcagtt cctgctcgat ccggcgacga tcggtgatgt cggtcgcgac
     4981 cagcacgaac aggcggtgcg ggctgcccgg ctcgttgatc gcgttggact gcatcgcgca
     5041 caggaattcc cggccgtcct tgcgccgctg ccacatctcc ccggcccagt gcccctgttc
     5101 gagcatcgcc gcccgcgact gcgcgtagaa cgccgcatcg tgctggtcgc tgttggtcag
     5161 cgatgcgtcg ttgcccacca cctccgccag ctcgtagccg ctcatgcggg tgaaggcggg
     5221 gttgaccatg acgtagcgga attcgtcgtc gatcaccacc accgcctcgg ccatgttgtg
     5281 catgacctcg gtcgcgatcc ggcgctcgcg ttcgtgctcg cgttgcgcgc tgacgtcgcg
     5341 cgcagtaccg gcgatgcggg tgatgcggcc cgcgtcgttg cgcgagactg cgcgaccgcg
     5401 cgcgcgcatc cagcgccagc cgtcggtgcc gtcctgcacg cgatgctcgg acaggaacat
     5461 cggggcatcg ccacggacgt aggcccgcag tcgatccagc accagcggga ggtcgtcggg
     5521 atggatctgg tggtcggcgt cgaggtcgat ccgcaggttc aggtcgtcct tgcggccagg
     5581 ctcgatccgg atgctctcca gttcacggcg ttccaggtcg tactgccagt aatactcgct
     5641 ggatgcccac aacgccaggc gcagtttttc ttcgcgctcg cgcagggcgc gcaggtgttc
     5701 gcgctgttcg cgctcgcggc ggcgatgccc acccaatacg acgcccgcca gcgccagcac
     5761 gagcagggcg agcaacagtg ccatgtaatg cgtggcgggc atctggccag tgtaggcagg
     5821 cgggcgggca gcggacaagc atcgcggcca ttgcgattgc tacactgggg ccccgtggtc
     5881 gagagtgcgc gcgtgagcga aaccgagctg ccgggttggg ccgacgtggc cgcggccgcg
     5941 gatcgcctgg acctggccag tacgccggcc gagctgcatg gcggtttgtg cggctggctg
     6001 gccgctggcg gcgacgacgc gccttcctgg ctcgcgctgg tgctggcgga tccgtcgttg
     6061 ccggtccccg atcgcggcgg cgtgctggac cgcctgcgca ccgccagcgc ggcgcaactg
     6121 tgcaatgccg atttcggctt cggcttgctg cttcccgacg atgacgacgt ggccgaacgc
     6181 gcgggcgcga tgttcgcctg gtgccgggca ttcctcggcg gcttcgggct cgcggtcggc
     6241 gggaagaagc tgtcggagga cggcgaggaa gcgctgggcg acatcgccaa cctcgccgcc
     6301 gcgagggtcg acgacgtcga cccggaaggc gacgaggaat cgctgaccga gatcgaggaa
     6361 tacctgcgga tggcggtcct gctgctgcat gccgactgca cgctggctcc gcggcagcgg
     6421 cagcgcctgc actgaatgcg ctgcacctcc tccgagcgca aggcattggc ccggcgccgc
     6481 aaggcgttga tgcaggccgc gggtgacggc gcgatcctgg tcctgccggc tgcgccggag
     6541 cgcatccgca gccgcgacac ccattacccg taccggcagg actcggactt ctggtacctg
     6601 accggctgca acgaaccgga ggcggtgctg gtgctggtgc ccgggcgccg gcacggcgaa
     6661 tcgatcctgt tctgccggga acgcgatccg gagcgcgagg gctgggatgg cccgcggctc
     6721 gggccggaag gtgcggtcga cgcgctcggg atggatgatg cgtatccgat ctccgacatc
     6781 gacgagatcc tgcccggcct gctggaaggc cgccgccggg tgcactacca cctcggccgc
     6841 gacgccgaat tcgacctcaa gctgatcggc tggatcaacc gggtgcgggc gcagtcgcgg
     6901 cagggcgcgc aaccgccgca ggagttcctg gcgctcggcc acctgctcga cgagatgcgc
     6961 ctgttcaagt ccgccgacga agtgaagctg atgcagcgcg ccgccgacat cagcgtgctg
     7021 gcgcaccggg cggcgatgcg cgcggcgcgc ccgggcatcc acgaatacga actgcaggcg
     7081 gagctggagc gcgtgttccg catgcacgac gcgcagccgg cttacgccag catcgtcggc
     7141 gccggcgcca atgcctgcgt gctgcactat cgcgacaatg ccgccagatc gcgcgacggc
     7201 gacctggtgc tggtcgatgc cggtgccgaa taccgcggct acgcggccga catcacccgc
     7261 acgttcccgc tcagcggccg cttcacccgc gagcagcgtg cattgcatga cctcgtcctc
     7321 gccgcacagg cggcagcact ggcgcaggcg cgccccgggg tgccttacga agccgggcat
     7381 gaggcggcgg tggagacgct gaccgagggc ctgctgtcgc tcgggctgct gaagggcagg
     7441 ctggaggcgg cgatcgcggc cggcgactac aagcgcttct accggcacaa gaccgggcac
     7501 tggctggggc tggacgtgca cgatgtcggc gaatacagga tcgacggcga atcgcgcctg
     7561 ctggagcccg gcatggtgtt caccatcgag cccggcctgt acatccccga ggacgatgtc
     7621 ggcgtgccgg cgaagtggcg cggcatcggc atccgcatcg aggacgacgt gctggtcacc
     7681 gccgacggcc atcgggtgct gaccgatgcc ctggagcgca gtgcggatga aatagaaacc
     7741 ttgatggcgg ccgcgaaacc ctgaccaggt gaacgcgccg cgcccggggt atgtcgagcg
     7801 cgccatggat ggcgcgcggc cgcgaatcgt agccggacgc gaagcctgag cggcgacata
     7861 ccccgggcgc ggcgcgttcg ccgtgcgaca ggcttcaagc cgtcgcgaac gcttcccgcg
     7921 tcaggttgag cggcacgccc tcggtgca
//
LOCUS       DAORUK010000094         7923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_10211_length_7923_cov_20.020336, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000094 DAORUK010000000
VERSION     DAORUK010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10211_length_7923_cov_20.020336"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PK818_07300"
     CDS             <1..183
                     /locus_tag="PK818_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82571.1"
                     /translation="ADSHFGESGQVHVIRTPRHVGTLSPIVHTIPVQLLAYHVALARG
                     TDVDKPRNLAKSVTVE"
     gene            complement(187..2316)
                     /locus_tag="PK818_07305"
     CDS             complement(187..2316)
                     /locus_tag="PK818_07305"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HQY82572.1"
                     /translation="MPIDPGRRRLLKTLVASAAALPFASCLALAGDVQVPTDQALFPQ
                     GVASGDPRPDRVLLWTRAPAASGDVRVRLQVALDPGFTQLLVERDLLARADADHCLRV
                     RIEGLAAGSDYHYRFLRKTAGGWRSSRHGRTRTAPAADSTAPVRFGVACCQHFEGRWY
                     NSLHALLADPPDFLLHLGDAIYEYAGDPAASGERHIAFDDAAGAISDGAHGNPCARSL
                     DNYRQLHRIYRSDALWQRLLATVPLVAIWDDHEFANDCWQDVATYGNGLRDERDTQRR
                     RNAEQAYFEFMPVDIDVGATGADGAQAVDAGLLFPQTRLWRELRFGRDAHLFLTDYRS
                     ARPDHPIPEDAFPGGLAYDEAALREWLPKAGIDFGTVSKLLLPYVDQSHPLWPKWRKP
                     LQRALNRGYRDAGLDKRAAARRVRAILAAPIALMVLRKLLERYNDAVPGFMRAPLPDR
                     DGSGLPLGLPWVAMGKTGLFAELGCRYFVVKDSYDLLAALRDAQGEAPALGQAQAAWL
                     RERLAASDARWKIVASSVAMVPMVVDLARPDLEAPPLYRRRFYLNVDQWDGFPRQRRA
                     LLDTFDAAGGVVLLSGDIHAGFAAQHTPRTVEFTLPAVSSTTLHEMSSNSAAADADNA
                     EAGKRMVAALESLLLEGDPGLRYVQSRRHGVGTARVDAGAFAFEVTELPATLCRTCHY
                     EDGAAVLAQASVRRFAVDAATHTLEDG"
     gene            2459..4978
                     /locus_tag="PK818_07310"
     CDS             2459..4978
                     /locus_tag="PK818_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY82573.1"
                     /translation="MSATFQQQPAPRLRQLSIALHLALLAAPLLPMAALAQDAPAAPA
                     APPQDTPATLDTIVVTAQKREQQVQDVPIAISAYSGEFLQATGIDGFEQLGNYVPGLQ
                     TQVQSPNNPGFVIRGVTSDSGDAQVAPRVSVFQDGVSISRSRGSVVELFDMQRVEILR
                     GPQGTLFGRGAQIGAVHLIQNKAVNASEGAFRAGFGTYGETYFNGFVNAPLAEDKLYG
                     RLAVFHQQRDGYVDNLAGGDLNGKDTFAVRGSLRLDLGEASGLDLIVNHQKDTPPGTA
                     FRSTGYYTPVRPRIFIAPLPTRGGSTDVFDRTADLNRGDDLGLDRTVTGVSLLGQFWL
                     SDDWSLSTITGWREFDSHEEFDGDGSQLSLIESAEIAEGRQFSQEVRFNFDAGGAFRG
                     FVGGSWFSESGSQQVPIRTDERSLYALLSPVLAPQINQAICVGNGGSWGSAGCNIPTP
                     PLPQVPVLYPLNPDGTPNTGFSLGGLPGLPNPGVPGLPAFFPLNPNHVEQYTNYGEVE
                     AIELFADGTWSVNERLDLTAGLRWTHEKLTSGYQADYAGVPSWFGFVLATPPAFPNIL
                     FAPTARINASKSYDSVVGRVVASYKFSDDVNGYASYSRGQRPDVLQVGGAGVEEVPAE
                     TVDSFEAGLKGSAADGRLVYDFAAYHYSYTNFQTAVPGTIRPLTSNGGNATAKGLEAS
                     LIGRFSDHVSGFLNYGWTHARFDDRDDDGNPQLLAGNQFRLTPENTVAMGLDVRVPFA
                     GGHEFFVRPNYTWRSRVYFEDINNDDYPGIDQDSYGLLNLNLGVTLANGWEVQAWGSN
                     LADEDYLIDAGNSGQQLGLPTYIVGAPRMYGLRVAYRFGGK"
     gene            5065..5727
                     /locus_tag="PK818_07315"
     CDS             5065..5727
                     /locus_tag="PK818_07315"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HQY82574.1"
                     /translation="MTPASKPWLAGIAVLASVAILGLYLPGMRVLFLLAKPLATLAVI
                     ALALRVHCDEPRYRRAIVAGLLLGLAGDVLLMLDGEPAFMAGLGSFLLGHLAYLHGYR
                     QRAPLFAAAWPFVLYALLAGVVLAWLWPSLPPGLRLPVLAYVAVLAAMAAQAAAVWWR
                     RRDAATACAAIGGACFLGSDAILAIDRFALPFVAAQALLLALYWTAQALIASSVQAPP
                     VR"
     gene            5832..6905
                     /locus_tag="PK818_07320"
     CDS             5832..6905
                     /locus_tag="PK818_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQY82575.1"
                     /translation="MAMLLLLLAGTGHSGFAGAVPGVPDTRSQTQAALALPEGLRLAL
                     DARLPDARGPELARVQQLIDFMIADDGLALRYQEHPTDGIAGSYAQRRVNCLSFTLMF
                     VALARASGLQARAQASGDALAMRVLGDTVFRTSHVNAGVRIDGRWHSVDVARRSLVTA
                     GEPEAITDAQAVALLHNNNAVEQLVRGDDAVATREIASALALDPESVTAWSNAGVVHW
                     RSGRREAAERAYLQALQLQRDHVGALGNLVGMYHSTGDAQRAAAYEKRLQRAQASDPF
                     SQFLIAQALAQSGANDDAVVHYRRAIRLMPDEPEFHRKLADSYEKQGKQDAARRSRQR
                     AGSLEAHRTAQRAIRTIDEPASG"
     gene            complement(6980..>7923)
                     /locus_tag="PK818_07325"
     CDS             complement(6980..>7923)
                     /locus_tag="PK818_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY82576.1"
                     /translation="GSSFANRFDTTRDSASLQGDFAVAAGQVLTAGFDWLRDRADVRD
                     PWSPFNAARGNRAVFAQYQGSFAAHDLQASLRRDDNDQFGGKTTGGIAWGYGFSDGWR
                     VTASHASAFKAPSFNELYYPYFGNPLLRPESARSSELSLRHDNGGWQWQLNAYRNDID
                     DLIVYDTAIFAANNIESARIRGVELTASGRLGAWDVRGQLGTLDARNLSTGGNHGKRL
                     PRRPQRSARIDLDRDLGDLGFGISGIAEGARWDDVANTLRVGGYGTVDARASWRFAAG
                     WTLQASLVNAFDRRYETTAYYNQPGREWSLGLRWQPR"
BASE COUNT         1153 a         2794 c         2776 g         1200 t
ORIGIN      
        1 gccgacagcc acttcggcga atccgggcag gtgcacgtga tccgcacccc gcgccacgtc
       61 ggcaccctca gcccgatcgt gcacaccatc ccggtgcagc tgctggccta ccacgtcgcc
      121 ctggcgcgcg gcaccgacgt cgacaagccg cgcaacctgg cgaagagcgt caccgtcgag
      181 tgaacctcag ccgtcctcca gcgtgtgcgt ggccgcatcc accgcgaagc ggcgcacgct
      241 tgcctgcgcg agcactgcgg ctccatcttc gtaatggcac gtccggcaca gggtcgccgg
      301 aagttcggtg acttcgaacg cgaaggcacc cgcatcgacg cgggcggtgc cgacgccgtg
      361 ccgcctggac tgcacgtaac gcaggcccgg atcgccctcc agcagcagcg actccagcgc
      421 cgcgaccatg cgcttgccgg cttcggcgtt gtcggcatcg gcggcggcac tgttcgacga
      481 catttcgtgc agggtggtcg aagacaccgc cggcagggtg aactccacgg tgcgcggggt
      541 gtgttgcgcc gcgaagccgg cgtggatgtc gccggacagc aggaccacgc cgccggccgc
      601 gtcgaacgtg tccagcagcg cgcggcgctg gcgcgggaag ccgtcccact ggtcgacgtt
      661 gaggtagaag cggcgccggt acagcggcgg cgcttcaagg tcgggacggg cgaggtcgac
      721 caccatcggc accatcgcca ctgaacttgc gacgatcttc cagcgcgcgt cgctggcggc
      781 caggcgttcg cgcagccacg ccgcctgcgc ctggcccagc gccggggcct cgccctgcgc
      841 atcgcgcagc gccgccagca ggtcgtagct gtccttgacc acgaagtagc ggcagcccag
      901 ttccgcgaac agcccggtct tgcccatcgc cacccacggc agccccaacg gcaggccgct
      961 gccgtcgcgg tccggcaatg gcgcgcgcat gaagccgggt acggcgtcgt tgtagcgctc
     1021 cagcagcttg cgcagcacca tcagcgcaat cggtgcggcg aggatggcgc gcacccgccg
     1081 ggccgccgcg cgcttgtcca ggccggcgtc gcggtagccg cggttgagcg cgcgctgcag
     1141 cggcttgcgc catttcggcc atagcgggtg cgactggtcg acgtagggca gcagcagctt
     1201 cgataccgtc ccgaagtcga tgcccgcctt cggcagccat tcgcgcagcg ccgcctcgtc
     1261 gtaggccagg ccgcccggga acgcgtcctc cgggatcggg tggtcggggc gcgcgctgcg
     1321 gtagtcggtc aggaacaggt gggcgtcgcg gccgaagcgc agctcccgcc atagccgggt
     1381 ttgcgggaac agcaggccgg catcgaccgc ttgcgcgccg tcggcgccgg tcgcgccgac
     1441 atcgatgtcc accggcatga attcgaagta ggcctgctcg gcattgcgcc gccgctgggt
     1501 gtcgcgttcg tcgcgcaggc cgttgccgta ggtcgccacg tcctgccagc agtcgttggc
     1561 gaactcgtgg tcgtcccaga tcgccaccag cggcaccgtc gccagcagtc gttgccacaa
     1621 cgcatcgctg cggtagatcc ggtgcaactg ccggtagttg tcgaggctgc gtgcgcaggg
     1681 attgccgtgc gcgccatcgg agatcgcgcc ggcggcgtcg tcgaatgcga tgtggcgctc
     1741 gccgctggcg gccgggtcgc cggcgtactc gtagatcgcg tcgcccaggt gcagcaggaa
     1801 atccggcggg tcggccagca gcgcgtgcag gctgttgtac cagcggcctt cgaagtgctg
     1861 gcagcaggcc acgccgaagc gcaccggcgc ggtgctgtcc gctgccggcg cggtgcgggt
     1921 gcggccgtgg cgcgagctgc gccagccacc cgcggtcttg cgcaggaagc gatagtggta
     1981 atcgctgccg gcggccagtc cctcgatgcg cacgcgcagg cagtggtcgg cgtcggcgcg
     2041 cgccagcagg tcgcgttcga ccagcagttg cgtgaaccca gggtccagcg cgacttgcag
     2101 tcgcacacgc acatcgccgc tggccgcggg tgcacgcgtc cacagcagca cccggtccgg
     2161 gcgcgggtcg cccgaggcca cgccctgcgg gaacagcgcc tgatccgtcg gtacctggac
     2221 gtccccggcg agcgcgaggc agctggcgaa gggcagggcg gcggcgctcg ccaccagggt
     2281 tttcagcagg cgacggcggc cggggtcgat gggcatgggc ggggtctggg tgggcgaaaa
     2341 cgtctaagat cgtgccgcga cgtttcaggg gtgggaccgg aacgtcaccg gacggcaaca
     2401 agcgctttcc acactctagc gtttgtcgcc gacaacaccg acttccttgg gggaaaccat
     2461 gagcgccact ttccagcagc agcccgcgcc gcgcctgcgc cagctttcga tcgccctgca
     2521 ccttgccctg ctggccgcgc cgctgctgcc gatggcggcg ctggcgcagg acgcgcccgc
     2581 cgcgccggcc gcgccgccgc aggacacgcc agcgaccctg gacaccatcg tggtcaccgc
     2641 gcagaagcgc gagcagcagg tgcaggacgt gccgatcgcg atctccgcgt actccggcga
     2701 gttcctgcag gccaccggca tcgacggctt cgagcagctc ggcaactacg tgcccgggct
     2761 gcagacccag gtgcagagcc cgaacaaccc cggtttcgtg atccgcgggg tgacctccga
     2821 cagcggcgat gcacaggtgg cgccgcgcgt gtccgtgttc caggacgggg tctcgatcag
     2881 ccgctcgcgc ggctcggtgg tggaactgtt cgacatgcag cgggtggaga tcctgcgcgg
     2941 cccgcagggc accctgttcg gccgcggcgc gcagatcggc gcggtgcacc tgatccagaa
     3001 caaggcggtg aacgccagcg agggcgcctt ccgcgccggc ttcggcactt acggcgagac
     3061 ctacttcaac ggcttcgtca acgcgccgct ggccgaagac aagctgtacg gccggctggc
     3121 ggtgttccac cagcagcgcg acggctatgt cgacaacctc gccggcggcg acctcaacgg
     3181 caaggacacc ttcgcggtgc gtggcagcct gcgcctggac ctcggcgagg cgtccgggct
     3241 ggacctgatc gtcaaccacc agaaggacac accgccgggc accgcgttcc gcagcaccgg
     3301 ctactacacc ccggtgcgcc cgcgcatctt catcgcgccg ctgcccacgc gcggcggcag
     3361 caccgacgtg ttcgaccgca ccgccgacct caatcgcggc gacgatctcg ggctcgaccg
     3421 cacggtcacc ggcgtcagcc tgctcggcca gttctggctg agcgacgact ggtcgctgtc
     3481 caccatcacc ggctggcgcg aattcgattc gcacgaagaa ttcgatggcg acggttccca
     3541 gctttcgctg atcgaatccg ccgagatcgc cgaaggcagg cagttcagcc aggaagtgcg
     3601 cttcaacttc gatgccggcg gcgcgttccg cggcttcgtc ggcggcagct ggttcagcga
     3661 atccggttcg cagcaggtgc cgatccgcac cgacgaacgc agcctgtacg cgctgctgtc
     3721 gccggtgctg gcgccgcaga tcaaccaggc gatctgcgtg ggcaacggcg gtagctgggg
     3781 ttccgccggc tgcaacatcc ccacgccgcc gctgccgcag gtgccggtgc tgtatccgct
     3841 gaacccggat ggcaccccga acaccggctt cagcctcggc ggcctgccgg gcctgccgaa
     3901 cccgggcgtg cccggcctgc cggcgttctt cccgctgaat ccgaaccacg tcgagcagta
     3961 caccaactac ggtgaagtgg aggcgatcga actgttcgcg gacggcacct ggtcggtcaa
     4021 cgaacgcctc gacctcaccg ccggcctgcg ctggacccac gaaaagctga cctccggcta
     4081 ccaggccgac tatgccggcg tgccaagctg gttcggcttc gtgctggcca ccccgccggc
     4141 gttcccgaac atcctgttcg ccccgaccgc gcgcatcaat gcctcgaaga gctatgactc
     4201 ggtggtcggc cgcgtggtcg ccagctacaa gttcagcgac gacgtcaacg gctatgccag
     4261 ctactcgcgc gggcaacgcc cggacgtgtt gcaggtcggc ggcgctggcg tggaagaggt
     4321 gccggcggag accgtcgaca gcttcgaggc agggctcaag ggctccgccg cggacggccg
     4381 cctggtctac gacttcgccg cctaccacta cagctacacc aacttccaga ccgcggtccc
     4441 gggcaccatc cgcccgctca ccagcaacgg cggcaatgcc accgccaagg gcctggaagc
     4501 atcgctgatc gggcgcttca gcgaccacgt cagcggcttc ctcaactacg gctggaccca
     4561 cgcccgcttc gacgaccgcg acgacgacgg caacccgcag ctgctggccg gcaaccagtt
     4621 ccggctgacc ccggagaaca ccgtggcgat ggggctggac gtgcgcgtcc cgttcgccgg
     4681 tggccacgag ttcttcgtgc gcccgaacta cacctggcgt tcgcgggttt acttcgagga
     4741 catcaacaac gacgactatc ccggcatcga ccaggacagc tacggcctgc tcaacctcaa
     4801 cctcggggtg acgctggcga acggctggga agtgcaggcg tggggctcca acctcgccga
     4861 cgaggactac ctgatcgacg ccggcaacag cgggcagcag ctcggcctgc cgacctacat
     4921 cgtcggcgca ccgcggatgt acgggctgcg cgtggcctac cggttcggcg gcaagtaacc
     4981 gctcccgcgg gtgtcgtgcg gggcccgctg cggcgggcct cgcgcgttgt ggcgtggcga
     5041 tacccgcatc gcgcacactg ggccatgacc cctgcatcga aaccctggct ggccggcatc
     5101 gccgtgctgg cgtcggtcgc catcctcggc ctgtacctgc ccgggatgcg cgtgctgttc
     5161 ctgctcgcca agccattggc cacgctcgcg gtgatcgcgc tggcgttgcg cgtgcactgc
     5221 gacgagccgc gctaccggcg cgcgatcgtg gccggcctgc tgttgggcct ggccggcgac
     5281 gtgctgctga tgctggacgg cgaacccgcg ttcatggccg ggcttggcag tttcctgctc
     5341 gggcatctgg cctacctgca cggctaccgg caacgcgcgc cgctgttcgc cgcggcctgg
     5401 ccgttcgtgc tctacgcgct gctggcgggg gtggtgctgg cctggctgtg gccgtcgctg
     5461 ccgcccggcc tgcggctgcc ggtgctggcg tacgtggcgg tgctggcggc gatggcggcg
     5521 caggccgcag cggtctggtg gcgccgccgc gatgccgcga ccgcctgtgc cgcgatcgga
     5581 ggcgcctgtt tcctcggctc cgacgcgatc ctggcgatcg accgcttcgc gttgccgttc
     5641 gtcgccgcgc aggccctgct gctcgccctg tactggaccg cgcaggcgtt gatcgcatcc
     5701 tcggtacagg cacccccggt gcgctgaaag ccggccgggc cagtcactgg tgccgccgcc
     5761 cgatcggcgc atactggcgc cgactccctg ccgcgggtgc gcgcacccgc aacggaagga
     5821 cgatgcggcg gatggcgatg ctcctgttgc tgctggccgg caccggccat tccgggtttg
     5881 ccggcgccgt gcccggcgtg cccgacaccc gctcgcaaac gcaggccgcg ctcgcattgc
     5941 ccgaaggctt gcggctggcg ctcgatgcgc gcctgccgga tgcgcgcggg ccggaacttg
     6001 cgcgggtgca gcagctgatc gatttcatga ttgccgacga tggcctcgcc ttgcgctacc
     6061 aggagcatcc gaccgacggc atcgccggga gctacgcgca gcgcagggtg aactgcctgt
     6121 cgttcacgct gatgttcgtc gcgttggcgc gcgcttccgg gttgcaggcg cgtgcgcagg
     6181 catccggcga cgcgctggcg atgcgcgtgc tcggcgacac cgtgttccgt accagccacg
     6241 tcaatgccgg ggtaaggatc gacgggcgct ggcattcggt cgacgtggca aggcgttcgc
     6301 tggtcaccgc aggcgagcct gaggccatca ccgatgcgca ggccgtggcg ttgctgcaca
     6361 acaacaacgc ggtggagcag ttggtgcggg gcgacgacgc ggtcgcgacg agggagatcg
     6421 cgtcagcgct ggcgctcgat ccggagagcg tgacggcgtg gagcaatgcc ggcgtggtgc
     6481 actggcgctc cggccggcgc gaggccgcgg aacgcgccta cctgcaggca ttgcagctgc
     6541 agcgcgacca cgtcggcgcg ctcgggaacc tggtggggat gtaccacagt accggtgacg
     6601 cgcagcgcgc ggccgcctac gaaaagcgcc tgcagcgcgc gcaggccagc gatccgttct
     6661 cgcagttcct gatcgcgcag gcactggcgc aatccggggc gaacgacgat gcggtcgtcc
     6721 actaccggcg cgcgatccgg ttgatgccgg atgaacccga gttccaccgc aagctcgccg
     6781 attcctacga gaagcagggc aagcaggatg cggcccggcg ttcgcgccag cgcgccggca
     6841 gtctcgaggc gcatcgcacc gcgcagcgcg cgatccggac catcgacgag cctgcctcgg
     6901 gctgaccgcg caagaaagca ggcccggcat ccctgccggg cctgtcgcat ccctgcgttg
     6961 ccgctccgga aagcgtcgtt taccgcggct gccagcgcag gcccaggctc cattcgcggc
     7021 ccggctggtt gtagtacgcg gtggtttcgt agcggcgatc gaaggcgttg accaggctcg
     7081 cctgcaatgt ccatccggcc gcgaagcgcc agctggcgcg cgcgtccacg gtgccgtagc
     7141 cgcccacgcg caaggtgttg gcgacatcgt cccaacgcgc gccttcggcg atgccactga
     7201 tcccgaaacc gagatcgccg aggtcgcggt cgaggtcgat gcgcgcactg cgctgcggcc
     7261 ggcgcggcag ccgcttgcca tggttgccgc cggtgctgag gttgcgcgca tccagggtgc
     7321 cgagctggcc gcgcacgtcc cacgccccga gccggccgct ggcggtgagt tcgacgccgc
     7381 ggatgcgtgc gctttcgatg ttgttcgccg cgaagatcgc ggtgtcgtac acgatcaggt
     7441 catcgatgtc gttgcggtag gcattgagct gccactgcca gccgccgttg tcgtgccgaa
     7501 gcgacagctc gctgctgcgc gcggactccg gacgcagcaa cgggttgccg aaatacgggt
     7561 agtacagttc gttgaagctc ggcgccttga acgcgctggc gtggctggcg gtgacgcgcc
     7621 aaccatcgct gaagccgtag ccccaggcga tgccgccggt ggtcttgccg ccgaactggt
     7681 cgttgtcgtc gcggcgcagg ctggcctgca ggtcgtgcgc ggcgaacgag ccctggtatt
     7741 gcgcgaacac ggcgcggttg ccgcgcgcgg cgttgaacgg cgaccacgga tcgcggacgt
     7801 cggcgcggtc gcgcagccag tcgaagccgg cggtcagcac ctgtcccgcg gcaaccgcga
     7861 aatcgccctg caggctggcg ctgtcgcggg tggtgtcgaa gcggttggcg aagctgctgc
     7921 cga
//
LOCUS       DAORUK010000095         7785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_10487_length_7785_cov_17.551617, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000095 DAORUK010000000
VERSION     DAORUK010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10487_length_7785_cov_17.551617"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1573)
                     /gene="alaS"
                     /locus_tag="PK818_07330"
     CDS             complement(<1..1573)
                     /gene="alaS"
                     /locus_tag="PK818_07330"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004087615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HQY82577.1"
                     /translation="MNTPAKTTSDIRRDFLDFFASKGHTIVPSAPLVPGNDPTLLFTN
                     SGMVQFKDVFLGAEKRSYVRAADVQRCLRAGGKHNDLDSVGYTARHHTFFEMLGNWSF
                     GDYFKKEAIAWAWELLTEVWKLPRERLLATVYHTDDEAYAIWRDDIGLPEDRIIRIGD
                     NKGAPFASDNFWQMADTGPCGPCSEIFYDHGPGHFGGPPGSPDEDGDRFIEIWNNVFM
                     QFDRAADGTLTPLPAPCVDTGMGLERITAILQGVHSNYEIDLFQALVRHAADLTGTAD
                     LENKSLRVIADHIRACAFLIVDGVLPSNEGRGYVLRRIIRRALRHGWMLGVRQPFFHK
                     LVATLDALMGDAYPVLREGRATAERALLAEEERFAETLDSGMKIFEDVAGRVADQVIP
                     GGDAFRLYDTYGFPLDLTQDMARERGMTVDVAGFDAAMAQQKETARAAGKFGGGVQLP
                     AELVSRLAPTVFLGYDELAAGSLQVLALLKDGRPVEAVEAGDDAIVILDRTPFYAESG
                     GQVGDIGELDGVESRF"
     gene            complement(1729..2226)
                     /locus_tag="PK818_07335"
     CDS             complement(1729..2226)
                     /locus_tag="PK818_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HQY82578.1"
                     /translation="MTVPGGDDGVASSSAPKRRRPEPSPLQRALGLLTRREHSRQELA
                     RKLQQRGVEREQAAAAIDKLTEAGWQDDARFAESVVRSRAASGYGPAYIRAELATHGL
                     ASEAIASVLDGFDGDWTENARALVCRRHPLALDGDRDARCKAADLLLRRGFSMEQVRA
                     AVTSD"
     gene            complement(2223..3260)
                     /gene="recA"
                     /locus_tag="PK818_07340"
     CDS             complement(2223..3260)
                     /gene="recA"
                     /locus_tag="PK818_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HQY82579.1"
                     /translation="MDENKKRALAAALGQIEKQFGKGSVMRMGDRPVEVVETIGTGSL
                     MLDIALGIGGLPKGRVVEIYGPESSGKTTLTLQTIAECQKAGGTCAFIDAEHALDPVY
                     AQKLGVNLEELYLSQPDTGEQALEIADMLVRSNAMDMVVIDSVAALTPKAEIEGEMGD
                     QLPGLQARLMSQALRKLTGNIKRSNCMVFFINQLRQKIGIMMPGQSNEVTTGGNALKF
                     YASVRLDIRRIGSIKKGEEIIGNQTRIKVVKNKLAPPFKQVITEILYGEGISREGELI
                     DMGVDAKLVEKAGAWYSYDGERIGQGKENARQYLKDNPAAAAKLEAVLREKFVPEEAA
                     RDEGDVAGKDA"
     gene            complement(3433..4068)
                     /gene="lexA"
                     /locus_tag="PK818_07345"
     CDS             complement(3433..4068)
                     /gene="lexA"
                     /locus_tag="PK818_07345"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011269877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HQY82580.1"
                     /translation="MSLTDTQRAIHAFIADRIDAEGVPPSQSEIARAFGFSGVRAAQY
                     HLEALEAAGVVERTPGRARGLRLLQVPDMPQQLPLPADDSALHLPVLGQVAAGLPIGA
                     DIGSEQVVLMDRALFSPAPDYLLKVKGDSMRDEGIFDGDLIGVHRTTDARSGQVVIAR
                     IDDEITVKLLKIGQGRIRLLPRNPDYAPIEVLPGQDFAIEGLYCGLVRPNA"
     gene            complement(4121..4618)
                     /locus_tag="PK818_07350"
     CDS             complement(4121..4618)
                     /locus_tag="PK818_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HQY82581.1"
                     /translation="MIVPNDAELDAIARATGERLLAARQMLVTAESCTGGWIAKAVTD
                     VAGSSAWFDCGMAVYSYEAKQAMLGVNPHTLEIHGAVSRETVVEMVSGALVHSGATIA
                     VAVTGIAGPGGGTEDKPVGTVWVAWKRRGGYPRAEVFHFEGDRDAVRRQTVAAALRGL
                     EHQLA"
     gene            complement(4723..7422)
                     /locus_tag="PK818_07355"
     CDS             complement(4723..7422)
                     /locus_tag="PK818_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY82582.1"
                     /translation="MNRKQPQPKQLAVAVFAALAAMSALPAFAQDAAPAQQKQEEAKT
                     LDTMVVTAQKREEQMQDVPIVVTSLSQQALQDTGVRDIKDMQILVPGLTVTSTQNEAI
                     TTARIRGIGTVGDNLGLESSVGVVIDGVYRPRNSVGFGDLGELERIEVLKGPQGTLFG
                     KNTSAGVINVVTRRPDQEMGGEGEVTFGNYGALGLAGWFNAPLGEYAAFRVYAAHRDR
                     DGWMTVEPGVGPRSESEDYDQNFRTVRAKLLIQPSDNVEVLISADASKRDENCCTAVQ
                     LVSGGTAPIINGVGGGNAIAIPANPWARVAYSNRSTAQKIDDRGVQAEVNVDLDMFGG
                     ATFTSVTGGRDWDTVNGRDFDFTGADLLYSNPEESESYNRFKTFSQEFRLTGSTDKVD
                     WLVGMFYANEDLDKTESYRIGAGYEPYLSTLVGSSALPQMKAALDPTLAYLSAQYQLP
                     LTMSFNPLNPAIFMSQFSGQTYGTTFAGFGALDKYEQNAKSLALFTNNTWHATEALDV
                     TLGLRYTREKKSLESVYENPNNGLACAASLASPGAGVARLLAARITNLSQLPAAQQAA
                     LIGQLTTALATSAAGQSLIGGVLGRMCLPWSNAQHDGRVTEQSRSENEWSGTLKFAYR
                     WNEQAMTYFSAARGYKAGGFNLDRVQSATGFSDGAAGITPVDDTSFPGEFVDSYELGA
                     KTTWAGGNLLLNGALFHQTYSDFQLNSFLGTSFVVRSIPKVTSQGLDAELLWQATPGL
                     MLQTGLVYADTKYGKNIPGGDFTFPGQLYKLPGGQMSFAPEWSLSASLTYEWDIGSDM
                     EGRFNIGAKWLSEYNTGSDLDAEKMQDAYAVMNARIGIGSRDRKWQLELWGMNILDED
                     YVQVGFDGPLQAVGSPSPGDPMNTYNAFLGAPRTYGATLRFRF"
     gene            7643..>7785
                     /locus_tag="PK818_07360"
     CDS             7643..>7785
                     /locus_tag="PK818_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82583.1"
                     /translation="MSVEQHTPLMRQFFAAKAEHPDVLLFFRMGDFYELFYDDARKAA
                     RLL"
BASE COUNT         1226 a         2593 c         2596 g         1370 t
ORIGIN      
        1 tgaagcggct ctcgacaccg tcgagctcgc cgatatcgcc gacctggccg ccggattcgg
       61 catagaaagg cgtgcggtcg aggatgacga tggcgtcgtc gccggcctcc actgcctcca
      121 ccggcctgcc gtccttgagc agcgccagca cctgcaggct gcccgccgcc agctcgtcgt
      181 agccgaggaa gacggtgggt gccagccgcg aaaccaattc ggccggcaac tgcacgccgc
      241 cgccgaactt gccggccgcg cgcgcggttt ccttctgctg cgccatcgcc gcatcgaagc
      301 cggccacgtc cacggtcatg ccgcgctcgc gcgccatgtc ctgggtgagg tcgagcggga
      361 agccgtaggt gtcgtacagg cggaacgcat caccgccggg gatgacctgg tcggccaccc
      421 ggccggccac gtcctcgaag atcttcatgc ccgaatccag cgtctcggcg aaacgctcct
      481 cctccgccag caatgcgcgc tcggcggtgg cgcggccctc gcgcaggacc ggataggcgt
      541 cgcccatcag cgcatccagg gtggccacca gcttgtggaa gaacggctgg cgcacgccca
      601 gcatccagcc gtggcgcagg gcgcggcgga tgatccggcg cagcacgtag ccgcggcctt
      661 cgttggacgg cagcacgcca tcgacgatca ggaacgcgca ggcgcggatg tggtcggcga
      721 tcacgcgcag cgacttgttc tccaggtcgg cggtgccggt caggtcggcg gcatgccgga
      781 ccagggcctg gaacaggtcg atctcgtaat tggaatgcac gccctgcagg atcgcggtga
      841 tccgctccag gcccatgccg gtgtccacgc agggcgcggg cagcggggtc agggtgccgt
      901 ccgcggcgcg gtcgaactgc atgaagacgt tgttccagat ctcgatgaag cggtcgccgt
      961 cctcgtcggg cgagccgggc gggccaccga agtggcccgg gccgtggtcg tagaagatct
     1021 cgctgcacgg gccgcagggg ccggtgtcgg ccatctgcca gaaattgtcg gaggcgaacg
     1081 gcgcgccctt gttgtcgccg atgcggatga tgcggtcttc cggaagcccg atgtcatcgc
     1141 gccagatggc gtaggcctcg tcgtcggtgt ggtagacggt ggccagcagg cgctccctgg
     1201 gcagcttcca gacctcggtc agcagctccc aggcccaggc gatggcctcc ttcttgaagt
     1261 agtcgccgaa cgaccagttg cccagcatct cgaagaaggt gtggtggcgc gcggtgtagc
     1321 ccaccgagtc caggtcgttg tgcttgccgc cggcgcgcag gcagcgctgc acgtcggccg
     1381 cgcgcacgta gctgcgcttt tccgcgccca ggaacacgtc cttgaactgc accatgccgg
     1441 agttggtgaa cagcagggtg ggatcgttgc ctggcaccag cggcgcggac ggcacgatgg
     1501 tgtggccttt gctggcgaag aaatcgagaa agtcgcgacg gatgtcggaa gtggtttttg
     1561 ctggcgtgtt catgcgggca tcgaaggtgg cgggcagcat tccgcccgcg agtccgacac
     1621 gatgcggccg cgggttcgag ttggcaaccc aatggcccca gtttacagcc ccgcaggcgc
     1681 ctgtcggcct gccggcacgc caaccgaccc ccgccctgct gcgcggccct agtccgacgt
     1741 caccgccgcc cgcacctgct ccatcgaaaa ccctcgccgc agcaggagat ccgctgcctt
     1801 gcaccgcgcg tcgcgatcgc cgtccagcgc aaggggatgg cggcggcaca caagcgcacg
     1861 ggcgttctcc gtccagtcgc catcgaaacc gtcgagcaca ctggcgatgg cttcgctggc
     1921 aagcccgtgc gtggccagct cggcgcggat ataggccggg ccatagccac tggcggcgcg
     1981 gctgcgcacg accgactcgg cgaaacgggc gtcatcctgc cagcccgcct cggtcaactt
     2041 gtcgatcgcg gcagcggcct gttcccgctc cacgccgcgc tgctggagct tgcgcgccaa
     2101 ctcctggcgg gagtgctcgc gccgggtcag caggcccagc gcgcgctgca agggagaagg
     2161 ctccgggcgg cggcgcttcg gcgctgacga ggacgccaca ccgtcgtcgc cacccggcac
     2221 cgtcatgcgt ccttgccggc gacgtcaccc tcgtcgcgcg cggcttcttc cggcacgaac
     2281 ttctcgcgca ggaccgcttc cagcttggct gccgcggccg ggttgtcctt gaggtactgg
     2341 cgcgcgtttt ccttgccctg gccgatccgc tcaccgtcgt agctgtacca ggcgccggcc
     2401 ttctcgacca gcttggcatc cacccccatg tcgatcagtt cgccctcgcg gctgatgccc
     2461 tcgccgtaca ggatctcggt gatgacctgc ttgaacggcg gcgccagctt gttcttgacg
     2521 accttgatcc gggtctggtt gccgatgatc tcctcgccct tcttgatcga accgatgcgg
     2581 cggatgtcca gccgcaccga agcgtagaac ttgagcgcgt tgccgccggt ggtcacttca
     2641 ttgctctgcc cgggcatcat gatcccgatc ttctggcgca actggttgat gaagaacacc
     2701 atgcagttgc tgcgcttgat gttgccggtc agcttgcgca gggcctggct catcagccgt
     2761 gcctgcaagc caggcagctg gtcacccatc tcgccttcga tttcggcttt cggggtcagc
     2821 gcggcgaccg agtcgatcac caccatgtcc atcgcgttgg aacgcaccag catgtcggcg
     2881 atctccagcg cctgctcgcc ggtatccggc tgcgacaggt acaactcctc caggttgacg
     2941 ccgagtttct gcgcgtagac cgggtcgaga gcgtgctcgg catcgatgaa ggcgcaggtg
     3001 ccgccagcct tctggcactc ggcgatcgtc tgcagggtca gggtggtctt gccggaggat
     3061 tccggcccgt agatctcgac gacgcggccc ttcggcaggc caccaatccc cagtgcgatg
     3121 tccagcatca gcgagccggt gccgatggtt tccaccactt ccaccgggcg gtcgcccatg
     3181 cgcatcaccg agcccttgcc gaactgcttt tcgatctggc ccagggcagc cgcaagcgcg
     3241 cgcttcttgt tttcgtccat cgtggtgttc ctcaaggtcg gtgaatcggg aatgtgtgca
     3301 ggttaggcgg gacgtatcgc agaagttgcg acggcgcgga ccgcacccgc aaattgccct
     3361 gctggtgtct tggaagggat cggggaacac cgctgccgcg gctgggattt acgcgcgcgc
     3421 actgcggccg gatcacgcat tgggccgtac caggccgcag tagagacctt cgatcgcgaa
     3481 gtcctggccc ggcagcacct cgatcggcgc atagtcggga ttacgcggca gcaggcgaat
     3541 ccgcccctgg ccgatcttca gcagcttgac cgtgatctcg tcatcgatgc gggcgatcac
     3601 cacctggccg ctgcgcgcgt cggtggtgcg gtgcacgccg atcaggtcgc cgtcgaagat
     3661 gccttcgtcg cgcatcgaat cgcccttgac cttcagcagg tagtccggcg ctggcgagaa
     3721 cagcgcacga tccatcagga ccacctgctc ggaaccgatg tcggcaccga tcggcaggcc
     3781 ggcggcgacc tggcccagca ccggcaggtg cagggcgctg tcatcggccg gcaacggcag
     3841 ctgctgcggc atgtccggta cctgcagcag gcgcagcccg cgcgcgcgcc cgggggtgcg
     3901 ctccaccacg ccggcggctt ccagcgcctc gaggtggtac tgcgcggcac gcacgccgct
     3961 gaagccgaag gcacgcgcga tttccgattg cgagggaggc acgccttcgg cgtcgatgcg
     4021 gtcggcgatg aaggcgtgga tggcgcgctg ggtatcggtc aggctcatgg ttagtagtaa
     4081 tactactaac ggttttgccc gtcaatgggc ctggcgtgct tcaggcgagt tgatgttcga
     4141 gcccgcgcag cgcagcggca acggtctgcc ggcgcaccgc atcgcggtcg ccctcgaagt
     4201 ggaagacctc ggcgcgcggg taaccgccac gccgcttcca ggcgacccag acggtaccca
     4261 ccggcttgtc ctcggtgccg ccacccgggc cggcgatacc ggtcacggcg accgcgatgg
     4321 tcgccccgga atgcaccagc gcgccgctga ccatttccac cacggtttcg cggctgaccg
     4381 cgccatggat ttccagggtg tgcggattga cgcccagcat ggcctgcttg gcttcgtagc
     4441 tgtagaccgc catcccgcag tcgaaccagg ccgaggagcc ggccacgtcg gtgactgcct
     4501 tggcgatcca gccgcccgtg cagctttccg cggtgaccag catctgccgc gcggccagca
     4561 ggcgttcgcc ggtagcccgc gcgatcgcat ccagttcggc gtcgttgggg acgatcatgt
     4621 acatgggctc atcgcaaggg cgcggcgtcc gttgtagcgc aaggccaccc aaaaaaaaac
     4681 cggcccgcgt tggcgggccg gtcggtgacg cactccgcag gatcagaagc ggaagcgcaa
     4741 ggtggcaccg taggtacgcg gcgcgccgag gaaagcgttg taggtattca tcggatcgcc
     4801 ggggctcggc gaaccaacgg cctgcagcgg gccatcgaac ccgacctgca cgtagtcctc
     4861 gtcgaggatg ttcatgcccc acaactcaag ctgccacttg cgatcgcgcg agccgatgcc
     4921 gatgcgggca ttcatgaccg catacgcatc ctgcatcttc tccgcatcca ggtcggaccc
     4981 ggtgttgtac tcggacagcc acttggcgcc gatgttgaaa cgcccttcca tgtcggaccc
     5041 gatgtcccat tcgtaggtca acgacgccga cagcgaccac tcgggtgcga agctcatctg
     5101 gccgccaggc agcttgtaca gctgccccgg gaaggtgaag tcgccgcccg ggatgttctt
     5161 gccgtacttg gtgtccgcat acacaaggcc tgtctgcagc atcaggccag gggtggcctg
     5221 ccacagcagc tcggcatcca ggccttgcga ggtcaccttc gggatcgatc gcaccacgaa
     5281 gctggtgcca aggaagctgt tgagctggaa gtcgctgtat gtctggtgga acagcgcgcc
     5341 attgagcagc aggttgccgc cggcccaggt ggtcttcgca ccgagctcgt agctgtccac
     5401 gaattcaccg gggaacgagg tgtcgtctac cggggtgatg cccgcggcac cgtccgagaa
     5461 accggtggcg gactgcaccc ggtcgaggtt gaagccgccg gccttgtaac cgcgcgccgc
     5521 ggagaagtag gtcatcgcct gttcgttcca gcgataggcg aacttcaggg tgcccgacca
     5581 ttcgttttcg gaacgggatt gctcggtcac gcgcccgtcg tgctgggcgt tggaccaggg
     5641 caggcacatg cggccaagga cgcctccaat cagggactgg ccggcagccg acgtggcaag
     5701 cgccgtcgtc agctgtccaa tcagtgccgc ctgttgcgcc gccggcagct gcgacaggtt
     5761 ggtgatgcgc gccgccagca gccgcgcgac cccggcgccc ggactggcaa gcgaagcagc
     5821 gcaggccagg ccgttattgg ggttttcgta caccgattcc agcgacttct tctcgcgcgt
     5881 gtaacgcagg cccagggtca cgtcgagcgc ctcggtggcg tgccacgtgt tgttggtgaa
     5941 cagggccagg ctcttcgcgt tctgctcgta cttgtcgagg gcaccgaacc cggcgaatgt
     6001 ggtgccatac gtctgcccgc tgaactggga catgaagatg gccgggttca gcgggttgaa
     6061 ggacatcgtg agcggcaact ggtactgggc gctcaggtag gccaaggtcg ggtccagcgc
     6121 ggccttcatc tgcggcaacg cggacgaccc caccagggtc gacaggtagg gctcgtaacc
     6181 ggcgccgatc cggtagcttt cggtcttgtc gaggtcctcg ttcgcataga acatgcccac
     6241 cagccaatcc accttgtcgg tggatccggt caggcggaac tcctggctga aggtcttgaa
     6301 acggttgtag gactcgctct cctcggggtt ggaatacagc aggtcggcgc cggtgaagtc
     6361 gaagtcgcgg ccattgaccg tatcccagtc acgcccgccg gtgaccgaag tgaaggtggc
     6421 gccgccgaac atgtcgaggt cgacgttcac ctcggcctgc acgccgcggt cgtcgatctt
     6481 ctgggcggtg ctgcgattgc tgtaggccac ccgcgcccat ggattggcgg ggatggcgat
     6541 ggcgttgccg ccgcccacgc cattgatgat cggcgcagtg ccgccactga caagttgcac
     6601 ggcggtacag cagttctcgt cgcgcttgct ggcgtcggcg gaaatgagca cctcgacgtt
     6661 gtcgctgggc tggatcagca gcttggcgcg caccgtgcgg aaattctggt cgtagtcttc
     6721 cgattccgaa cgcggaccca cgccaggctc gaccgtcatc cagccatcgc ggtcgcggtg
     6781 cgcggcatac acgcggaatg cggcgtactc gccaagcggc gcgttgaacc agccggcaag
     6841 gccgagcgcg ccgtagttgc cgaaggtgac ctcgccctcg ccgcccattt cctggtcggg
     6901 gcggcgggtg accacgttga tgacgccggc cgaggtgttc ttgccgaaca gggtgccctg
     6961 cggacccttc aggacttcga tgcgttccag ctcgccgagg tcgccgaagc cgaccgaatt
     7021 gcgcggccgg tagacgccat cgatcaccac cccgaccgag gactccaggc ccaggttgtc
     7081 acccaccgtg ccgatgccgc ggatgcgggc ggtggtgatg gcttcattct gggtgctggt
     7141 gacggtcagg cccggaacca ggatctgcat gtccttgatg tcgcgcacgc cggtgtcctg
     7201 caaggcctgc tgcgacagcg acgtgaccac gatcggcacg tcctgcatct gctcttcgcg
     7261 cttctgcgcg gtgaccacca tggtgtcgag ggtcttcgcc tcttcctgct tctgctgcgc
     7321 gggtgcggcg tcctgggcga acgcggggag cgcggacatc gcggccagtg ccgcgaacac
     7381 ggcgacggcc agctgcttgg gttgcggttg cttgcggttc atggacttcc cctgggttga
     7441 gcacgccgga attccagccg tcgcggttgc gacggccgca ttttcatcga cgcgaccacg
     7501 tgggggtatg gcgcgcgggc agttttcgac tgccttgacg gaaaactaac acatccatac
     7561 gcgtgcgtgt ccagcaaccg ttgcagctgt aaccggggga tcgggcacac tacccggccc
     7621 cccgcttcgt cccgccccgc gcatgtccgt cgagcagcac accccgctga tgcgccagtt
     7681 cttcgccgcc aaggccgagc acccggatgt gctgctgttc ttccgcatgg gcgacttcta
     7741 cgaactgttc tacgacgacg cgcgcaaggc cgcgcggctg ctcga
//
LOCUS       DAORUK010000096         7715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_10623_length_7715_cov_20.609138, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000096 DAORUK010000000
VERSION     DAORUK010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10623_length_7715_cov_20.609138"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PK818_07365"
     CDS             <1..375
                     /locus_tag="PK818_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQY82584.1"
                     /translation="GVDIVGISHFGTFLKNTENYRRDLRRVEYGDERDPAMAAVFERI
                     SPLNNAHRITSPLFVAQGRNDPRVPWTEAEQIVKAVRGNGQPVWYLLYADEGHGFAKK
                     SNNDWFGAATILFWQQHLLGGE"
     gene            complement(422..1276)
                     /gene="ada"
                     /locus_tag="PK818_07370"
     CDS             complement(422..1276)
                     /gene="ada"
                     /locus_tag="PK818_07370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HQY82585.1"
                     /translation="MTQSNPPSTPNRHAALIARACRRIEAAEQPPSLDDLAQDAGLSP
                     FHFHRVFKAATGLTPKAYATAHRANAVRASLARGKASVTDAIFDAGFNANSRFYEQAD
                     AMLGMKPAQYRAGGADARIAFAIGQCSLGAILVARSARGVCAISLGDDPDALLGELQD
                     RFPRAELIGGDAGFERLVAQVVGLIEQPNIGHELPLDIRGTAFQQRVWQALRQIPAGE
                     TASYAQIAQRIGSPKSVRAVAGACAANTLAVAIPCHRVVRSDGALSGYRWGVERKREL
                     LSREHADR"
     gene            complement(1359..2003)
                     /gene="alkB"
                     /locus_tag="PK818_07375"
     CDS             complement(1359..2003)
                     /gene="alkB"
                     /locus_tag="PK818_07375"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HQY82586.1"
                     /translation="MSDLFAAHHPGTRTDLAAQACVLHGFALGVVDALLADIRAIEAS
                     APFRHLVTPGGHVMQVAMTNAGTFGWCSDRRGYRYDRLDPQSGKPWPALTPAFLRLAA
                     DAAREAGFDGYVPDACLVNRYAPGTRLSLHQDRDEDDRVAPIVSVSLGLPATFLFGGF
                     ARSDKTVRVPLTHGDVVVWGGSDRMRFHGVLPVKPGRHDALGELRLNLTFRKAR"
     gene            complement(2011..2943)
                     /locus_tag="PK818_07380"
     CDS             complement(2011..2943)
                     /locus_tag="PK818_07380"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HQY82587.1"
                     /translation="MATPNPGVIRQYWSLTKPRVVALIVFTAVIGMFLAVPGLPPLRE
                     AALGTLGIWLAAASAAAINQILDARIDARMARTSWRPLVAGTLTMRQALVFALVLAAA
                     SMLLLALGVNQVTAWLTFASLIGYAVVYTRFLKRATPQNIVIGGIAGAAPPLLGWSAV
                     TGMQGEWDWAHALLLVLIIFAWTPPHFWALAIFRREDYARALVPMLPVTHGVEYTRWQ
                     ILLYTVLLVIATVLPWATHMSGLFYLGGALVLGAVFLWYAWKLLDPPDEFFAMRAFRY
                     SIVYLMALFGFLLLDHWLLPFLQPAPAMEFAPTA"
     gene            complement(2948..4117)
                     /locus_tag="PK818_07385"
     CDS             complement(2948..4117)
                     /locus_tag="PK818_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HQY82588.1"
                     /translation="MNPDNPASRTALHKPAVYRHFHRIAWLAVALAACVIVFGAFVRL
                     SDAGLSCPDWPTCYGKAAWPTHAAQIVDHAATDIRPVEPSKAWREQFHRHIAAGLGLL
                     VFALALLAARRRRFGLAQVVGAAALVAVAIPLYMQGEYVTSSVLVAIAEAVLLWAALR
                     WSNFDLARTAVLTLAVIIFQAMLGMWTVTWLLKPAVVMAHLLGGLTTFSLLAWMAWRA
                     TDIPIRVGDAGRLRRLLVVGLVLLGVQIALGGWTSANYAALACGTDYPKCAGEWWPAR
                     DFREGFVLWRGIGVDYEGGILDNQARIAIQLAHRMVAILVTGHLLVLALRLLRSPGLR
                     GWGGLLGILLVAQVALGIANVVMSLPLHVATAHNAGATALLFVLVTLLARVRAPD"
     gene            complement(4123..4704)
                     /locus_tag="PK818_07390"
     CDS             complement(4123..4704)
                     /locus_tag="PK818_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82589.1"
                     /translation="MTPPSAADAIRRRNRRMLLALFLLFFGGMLVAGVLRFSGWRPEG
                     SKNRGEMLQPYGDLRGYVPTLARGDAYRWNDEPRIWRIAAIPRDCDGARADPCTRLLA
                     DLDKVWRLMGKDADRVHILWAGAVPRGLVLPGEVRVLRADDGLRAGLVRWDDAGGDAA
                     WLVDPNGFVVLRYAPGFDPGDLRTDLARLLKIN"
     gene            complement(4701..5438)
                     /locus_tag="PK818_07395"
     CDS             complement(4701..5438)
                     /locus_tag="PK818_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HQY82590.1"
                     /translation="MLGWALALAAIVVFAGFGRWQLGRMHDKQAKLEAAARALDRRAP
                     QPLLLASDPKRARAYDWAAGSGSIGGRTVWLDNQLRAGRAGVRMYCVLLPDDGVQALL
                     VDAGWWPLDASRTLPVFGCPLGRGVAVRGLLAPPPSSGLARGDALAAAGPQRWLATRL
                     DLAVIARELKLSTGIAPRVLRLDPARNKGDAGVMLAPGERDLDILPNTLTPERHLGYA
                     VQWFGLALAVLVAAIILTLRNRKRRSP"
     gene            5535..5762
                     /locus_tag="PK818_07400"
     CDS             5535..5762
                     /locus_tag="PK818_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HQY82591.1"
                     /translation="MNDSLKTLLIVGVLILILWNLGAGLYYMLVDKGQTKRTVNSLTR
                     RIGLSIALVLFVVLAIYMGWITPHGVGRSPG"
     gene            complement(5847..6746)
                     /locus_tag="PK818_07405"
     CDS             complement(5847..6746)
                     /locus_tag="PK818_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014501710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HQY82592.1"
                     /translation="MATAHANTATDHGTDAAAYYVPHGSRWPVFASVALFVTMAGLAS
                     WLNEASWGRATFFAGLAGLLAVLFKWFGDVIRESLAGHYNKQVDTSFRMGMVWFIFSE
                     VMFFAAFFGALFYARQFALPWLNGEGDGMATHSLLWSNFAGGWPSNGPGAVGGAYQTI
                     PAWGLPLLNTLILLTSGVTVTIAHHALKEGKRGALLLFLGLTVLLGGLFLFFQAEEYI
                     HAYRELNLTLGSGIYGSTFFMLTGFHGAHVTLGTIMLAVMWLRCLRGHFSRDDHFAFE
                     AVAWYWHFVDVVWLGLFLFVYVL"
     gene            complement(6793..7368)
                     /locus_tag="PK818_07410"
     CDS             complement(6793..7368)
                     /locus_tag="PK818_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HQY82593.1"
                     /translation="MSRNRSFGVAKMAGVALAAFGFAFALVPLYRIACEKVFGVRLEQ
                     GPARVVAESDARQQRWVTVQFDGGVNSKLPWQFSPGQDSMRVLVGRQYEATYSARNTA
                     DRAIVGSAVPSVAPARASGYFSKTECFCFTAQTLQAGEARDMPVRFIIDPALPAGVKT
                     VTLSYTFYKNDALTAEINAPRSHAGAARAAP"
     gene            complement(7365..7505)
                     /locus_tag="PK818_07415"
     CDS             complement(7365..7505)
                     /locus_tag="PK818_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82594.1"
                     /translation="MREHPATMQQADAAPRRKAARRTAMLLGAIAVAVYAGFILMGVL
                     GQ"
     gene            complement(7513..>7715)
                     /locus_tag="PK818_07420"
     CDS             complement(7513..>7715)
                     /locus_tag="PK818_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014501708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HQY82595.1"
                     /translation="FGMFATPFIMFFVLWHSLKHGAKAEARAWEGAKGLEWTVPSPAP
                     HHTFATPPVIRPGDLAHGDITH"
BASE COUNT         1290 a         2735 c         2699 g          991 t
ORIGIN      
        1 ggcgtggaca tcgtcggcat ctcgcacttc ggcaccttcc tgaagaacac cgagaactac
       61 cgccgcgacc tgcgccgcgt cgaatacggc gacgagcgcg acccggcgat ggccgcggtg
      121 ttcgagcgca tctccccgct caacaacgcg cacaggatca cctcgccgct gttcgtggcg
      181 cagggccgca acgacccccg cgtgccgtgg accgaggcgg agcagatcgt caaggcggtg
      241 cgcggcaacg gccagccggt gtggtacctg ctgtacgccg acgagggcca cggcttcgcg
      301 aagaagtcga acaacgactg gttcggcgcg gccaccatcc tgttctggca gcagcatttg
      361 ctgggcggcg agtagccacg ggcgcgacca acgtgtcgcg ctacgctccg gccaactcac
      421 ctcaccggtc ggcgtgctcc cgcgacaaga gttcgcgctt gcgctccacc ccccagcgat
      481 agccggacag cgcgccatcg ctgcgcacca cgcgatggca gggaatcgcc accgccagcg
      541 tgttcgccgc acatgcaccg gcgaccgcgc gcaccgactt gggcgaaccg atgcgttgcg
      601 cgatctgcgc gtagctcgcg gtctcgccgg ccgggatctg ccgcaatgcc tgccagacgc
      661 gctgctggaa ggcggtgccg cgaatgtcca gcggcagctc gtggccgatg ttgggctgtt
      721 cgatcagccc gaccacctgc gcgaccaaac gctcgaaacc ggcatcgcca ccgatcagct
      781 cggcgcgcgg gaagcggtcc tgcaattcac ccagcaacgc gtccggatcg tcgcccagcg
      841 agatcgcgca cacgccgcgc gcgctgcgcg cgaccaggat cgcgcccagc gagcactggc
      901 cgatcgcgaa tgcgatccgc gcgtccgcgc caccggcacg gtattgcgcg ggcttcatgc
      961 ccagcatcgc atccgcctgc tcgtagaagc ggctgttggc gttgaagccg gcatcgaaga
     1021 tcgcatcggt cacgctggcc ttgccccgcg cgagcgacgc acgcacggca ttggcgcgat
     1081 gcgccgtggc ataggccttc ggcgtcagcc cggtggcggc cttgaacacg cggtggaagt
     1141 ggaacggact gaggccggcg tcctgcgcca ggtcgtccag cgacggcggc tgttccgcgg
     1201 cctcgatccg ccggcacgcc cgtgcgatga gcgcggcatg cctgttcggc gtcgatggcg
     1261 ggttggactg ggtcatggcg tgcatggcga tgttgcccga tggcgtgccg ccactctagg
     1321 gcgcggcgcc aggccgggca ctccggttct tgcgatgctc acctggcctt gcggaaggtc
     1381 aggttcaagc gcagctcgcc cagcgcatcg tgccgacccg gcttcaccgg caacacgccg
     1441 tggaagcgca tgcggtccga gccgccccag accacgacat cgccatgcgt gagtggcact
     1501 cgcacggtct tgtcgctgcg tgcaaagcca ccgaacagga aggtggccgg cagccccagc
     1561 gagaccgaca cgatcggcgc gacgcggtcg tcctcgtcgc gatcctggtg cagcgacagc
     1621 cgcgtacccg gcgcgtagcg attgaccagg caggcatccg gcacgtagcc gtcgaagccg
     1681 gcttcgcgcg cggcgtcggc agcaagacgc aggaacgccg gcgtcaatgc gggccaaggc
     1741 ttgccggatt gcggatcgag ccggtcgtag cgatagccgc ggcggtcgct gcaccagccg
     1801 aaggtgccgg cattggtcat cgccacctgc atgacgtggc cgccgggcgt gaccaggtgg
     1861 cggaacgggg cacttgcctc gattgcgcgg atgtcggcca gcaaggcatc gacgacaccg
     1921 agcgcgaagc cgtgcagcac gcaggcctgc gcggcgaggt cggtacgcgt gccggggtgg
     1981 tgcgcggcga acaggtcgct catcggcgca tcaggccgtg ggcgcgaatt ccatcgccgg
     2041 cgccggctgc aggaacggca gcagccagtg gtcgagcagc aggaacccga acagcgccat
     2101 caggtacacg atcgagtagc ggaaggcacg catggcgaag aactcgtccg gcgggtcgag
     2161 cagcttccac gcgtaccaca ggaacaccgc gcccagcacc agcgcgccac cgaggtagaa
     2221 cagcccgctc atgtgcgtgg cccacggcaa caccgtggcg atcaccagca gcacggtgta
     2281 cagcaggatc tgccagcgcg tgtactccac gccgtgggtg accggcagca tcggcaccag
     2341 cgcgcgcgcg tagtcctcgc ggcggaagat cgccagcgcc cagaaatgcg gcggcgtcca
     2401 cgcgaagatg atcagcacca gcagcagcgc atgcgcccag tcccattcgc cctgcatgcc
     2461 ggtcaccgcc gaccagccca gcaacggcgg cgcggcgccg gcgatgccgc cgatcacgat
     2521 gttctgcggc gtcgcccgct tcaggaaccg ggtgtacacc accgcgtacc cgatcagcga
     2581 ggcaaaggtc agccatgcgg tgacctggtt caccccaagt gccagcagca acatcgatgc
     2641 cgccgccagc accagcgcga acaccagcgc ctgccgcatc gtcagggtcc cggccaccag
     2701 tggccgccac gacgtgcgcg ccatccgggc gtcgatccgc gcatccagga tctggttgat
     2761 cgccgccgcg ctggccgcgg ccagccagat gccgagggtg ccgagcgccg cctcgcgcag
     2821 cggcggcagc ccgggcaccg ccaggaacat gccgatcacc gcggtgaaca cgatcagcgc
     2881 aaccacccgc ggcttggtca ggctccagta ctggcggatc acgccagggt tcggcgtggc
     2941 catcgcgtca gtccggggcc cgcacgcgcg caagcagggt caccagcacg aacagcaggg
     3001 cggtggcacc ggcgttgtgc gcggtggcca cgtgcagcgg cagcgacatc accacgttgg
     3061 cgatgcccag cgcgacctgc gcgaccagca ggatccccag caggccgccc cagccgcgca
     3121 ggcccgggct gcgcagcagg cgcagcgcca gcaccagcag gtggccggtg accaggatcg
     3181 ccaccatccg gtgcgcgagc tggatcgcga tgcgcgcctg gttgtcgagg atgccgccct
     3241 cgtaatccac gccgatcccg cgccacagca cgaagccttc gcggaaatcg cgcgccggcc
     3301 accactcgcc ggcgcacttc gggtaatcgg tgccgcaggc cagcgccgcg tagttggcgc
     3361 tggtccagcc gccaagcgcg atctgcacgc ccagcagcac cagcccgacc accagcagcc
     3421 ggcgcaggcg gccggcatcg ccgacccgga tcgggatgtc ggtggcgcgc cacgccatcc
     3481 acgccagcag cgagaaggtg gtcaggccgc caagcaggtg cgccatcacc accgccggct
     3541 tcagcagcca ggtcaccgtc cacatgccaa gcatggcctg gaagatgatc accgccaggg
     3601 tcagcaccgc ggtgcgcgcc aggtcgaagt tgctccagcg cagcgccgcc cacagcagca
     3661 cggcctcggc gatcgcgacc agcaccgacg aggtgacgta ctcgccctgc atgtacaacg
     3721 ggatcgcgac cgcgaccagc gccgcggcgc cgaccacctg ggccaggccg aagcggcggc
     3781 ggcgcgcggc cagcagcgcc agcgcgaaca ccagcaggcc gaggccggcg gcgatgtggc
     3841 ggtggaactg ctcgcgccag gccttgctcg gctcgaccgg gcggatgtcg gtggcggcgt
     3901 ggtcgacgat ctgggcggca tgggtcggcc acgccgcctt gccgtagcag gtcggccagt
     3961 ccgggcagct gaggccggca tcggacaggc gcacgaacgc accgaacacg atcacgcagg
     4021 ccgccagcgc gaccgccagc caggcgatgc ggtggaagtg ccggtacacg gcgggcttgt
     4081 gcagggcggt gcgggaagcg ggattatcgg gattcatcac ggtcaattga tcttcagcag
     4141 gcgggcgagg tcggtgcgca agtcaccggg atcgaagccg ggagcgtagc gcaagaccac
     4201 gaagccgttc gggtccacca gccacgcggc atcaccgccg gcgtcgtccc agcgcaccag
     4261 ccccgcgcgc aggccgtcgt cggcgcgcag cacgcgcacc tcgcccggca gcaccagccc
     4321 gcgcggcacc gcgccggccc acaggatgtg cacgcggtcg gcatccttgc ccatcagccg
     4381 ccagaccttg tcgaggtcgg ccagcaggcg cgtgcatggg tccgcgcgcg cgccgtcgca
     4441 gtcgcgcggg atcgccgcga tccgccagat ccggggctcg tcattccagc ggtaggcgtc
     4501 gcctcgggcc agggtcggca cgtagccgcg caggtcgcca tagggctgca gcatttcgcc
     4561 ccggttcttc gagccctccg gccgccagcc ggaaaaacgc agcacgccgg ccaccagcat
     4621 gccgccgaag aacagcagga acagcgccag cagcatgcgg cggttgcggc ggcggatggc
     4681 gtcggcggcg gacggcggtg tcatggcgag cggcgtttcc ggttgcgcag ggtgaggatg
     4741 atggcggcaa ccagcaccgc cagcgccagc ccgaaccact gcacggcata gcccaggtgg
     4801 cgctcggggg tcagcgtgtt cggcaggatg tcgaggtcgc gctcgcccgg ggccagcatc
     4861 acgcccgcat cgcccttgtt gcgcgccgga tccagccgca gcacgcgcgg ggcgatcccg
     4921 gtcgaaagct tcagctcgcg cgcgatcacg gccaggtcca ggcgcgtggc cagccagcgt
     4981 tgcgggccgg cggcagccag tgcgtcgccg cgcgccaggc cgctggacgg cggcggcgcc
     5041 agcaggccgc gcaccgccac accccgcccg agcgggcaac cgaacacggg caacgtgcgg
     5101 ctggcgtcca gcggccacca gccggcatcg accaagagcg cctgcacgcc gtcgtcgggc
     5161 agcagcacgc agtacatgcg cacgccggcc ctgccggcac gcagctggtt gtccagccac
     5221 acggtgcggc cgccgatgct gccgctgccg gcggcccagt cgtaggcgcg ggcacgcttg
     5281 ggatccgagg ccagcagcag tggctgcggc gcccgcctgt cgagcgcgcg tgccgcggcc
     5341 tcaagcttcg cctgcttgtc gtgcatccgc cccagttgcc agcgtccgaa cccggcgaac
     5401 accacgatgg cggccagcgc cagcgcccag ccgagcagcc agccaccgcc gcgcggcgcc
     5461 gccgcgctca ccacgcgcac ccgggctgcg ataatgcggc gatcccgccg cggccaggcg
     5521 gcaacgaggg cgccatgaac gattcgctga agaccctgct catcgtcggt gtgctgatcc
     5581 tcatcctgtg gaacctgggc gccggcctgt actacatgct ggtggacaag ggacagacca
     5641 agcgcaccgt gaactcgctg accaggcgca tcggcctgag catcgccctg gtcctgttcg
     5701 tggtgctggc catctacatg ggctggatca cgccgcacgg cgtcgggcgc agcccgggct
     5761 gaaacggatc ggccgggatc cggcctggct gccgccgcgc gggccggaat caccggccca
     5821 cgtggcttga tcgatggcgt gcctgcttac aggacgtaca cgaacaggaa caggcccagc
     5881 cacaccacgt ccacgaagtg ccagtaccag gccaccgcct cgaacgcgaa atggtcgtcg
     5941 cggctgaagt gcccgcgcag gcagcgcagc cacatgaccg ccagcatgat cgtgcccagg
     6001 gtcacgtgcg caccgtggaa gccggtcagc atgaagaagg tcgagccgta gatgcccgaa
     6061 cccagggtca ggttcagttc ccggtaggcg tggatgtatt cctcggcctg gaagaacagg
     6121 aacaagccgc caagcagcac ggtcaggccc aggaacagca gcagcgcccc gcgcttgcct
     6181 tccttcagtg catggtgggc gatggtgacg gtcacccccg aggtcagcag gatcagggtg
     6241 ttgagcagcg gcaggcccca cgccgggatg gtctggtagg cgccgccgac cgcgcccggg
     6301 ccattgctgg gccagccgcc ggcgaagttc gaccacaaca ggctgtgggt cgccatgccg
     6361 tcgccttcgc cattgagcca cggcagcgcg aactggcggg cgtagaacag cgcgccgaag
     6421 aaggccgcga agaacatcac ctcggagaag atgaaccaca ccatgcccat ccggaacgag
     6481 gtatcgacct gcttgttgta gtggccggcc agcgattcgc ggatgacgtc gccgaaccac
     6541 ttgaacagga ccgccagcag gccggcgagg ccggcgaaga aggtggcgcg gccccagctg
     6601 gcctcgttga gccagctggc aagcccggcc atggtcacga acagcgcgac cgatgcgaac
     6661 accggccagc ggctgccatg cggcacgtag taggcggcgg cgtcggtgcc gtggtcagtg
     6721 gcggtattgg cgtgggcagt ggccatggcg gtcttccggg tggttcggtg caagggggcg
     6781 gatcggctgg aatcagggtg ccgcgcgcgc cgcgccggca tgggacctgg gcgcgttgat
     6841 ctcggcggtc agtgcgtcgt tcttgtagaa ggtgtaggac agggtgacgg tcttcacccc
     6901 ggccggcaac gccgggtcga tgatgaagcg caccggcatg tcgcgcgctt cgccggcctg
     6961 cagggtctgc gcggtgaaac agaagcactc ggtcttgctg aagtagccgg aggcgcgcgc
     7021 cggcgcgacc gaaggcaccg cgctgccgac gatcgcgcgg tcggcggtgt tgcgcgcgga
     7081 ataggtcgct tcgtactggc ggccgaccag cacgcgcatg ctgtcctggc ccgggctgaa
     7141 ctgccacggc agcttggaat tgacgccgcc gtcgaactgg acggtgaccc agcgctgctg
     7201 ccgcgcatcg ctttcggcca cgacccgcgc cgggccctgc tccaggcgca cgccgaacac
     7261 cttctcgcag gcgatccggt acagcggcac cagcgcgaac gcgaagccga acgcggccag
     7321 cgccacgccc gccatcttcg ccaccccgaa actgcggttg cggctcattg gcccagcact
     7381 cccatcagga tgaagcctgc gtacaccgcc accgcgatcg cgcccagcaa catcgccgtg
     7441 cgccgcgcgg ccttgcggcg cggggcggcg tctgcctgct gcatcgtggc ggggtgttcg
     7501 cgcatgccgg gatcagtggg tgatgtcgcc gtgggcaagg tcgcccgggc ggatcaccgg
     7561 cggggtggcg aaggtgtggt gcggggccgg gctgggcacc gtccattcca ggcccttcgc
     7621 gccttcccac gcgcgcgcct cggccttggc gccgtgcttg agcgagtgcc agaggacgaa
     7681 gaacatgatg aacggggtgg cgaacatgcc gaacg
//
LOCUS       DAORUK010000097         7654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_10744_length_7654_cov_17.352283, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000097 DAORUK010000000
VERSION     DAORUK010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7654
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10744_length_7654_cov_17.352283"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1878
                     /locus_tag="PK818_07425"
     CDS             <1..1878
                     /locus_tag="PK818_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HQY82596.1"
                     /translation="AWYDPADPGWGVMSHYFRGGDGASRVFRTVYFHDSAGAPRWAFA
                     QDTVDGLRQAQTYYTPYVECIDCAIAPILTTPIGSGTTRLTQPLAQADAARNRIELAL
                     RFDSGAPFARNTALALISEPLRVAGAAATAQGPLAGSVIDGGIESFVAIPYVAPPLGA
                     LRWRAPQAPALRERLLEARAIGPGCPQPAGQGFFSGAAARHDEDCLQLNVWRPATPGP
                     HPVMVWIHGGGLTQGSAVQLQNGVLLYDGAVYARRDVVFVSINYRLGPLGFLAQRDLR
                     GEAPDHPQSGNYGLLDQVAALAWVRANIAAFGGDPQRVTVYGESAGGVSSCVLLATPA
                     ASGLFQRAIVQSGNCLWNAPSLDAGIEQGDRVTLAAGCVTAPDRRACLRALSVAALFA
                     AGPPVISTGASTAPGEVYGLVVDGYVLPESPGPAIAGGRAAPLPLLIGVNDDEHATLA
                     PAASLPATAAGYEAAVRSRFGLIGGEVVARYPAAAYPTPALAYQDLLDDARFTCAARR
                     AGADHAARGNAVYQYVLTEILPDAGLVALESFHALDVLLLFGPRAQAQAPERTLAARM
                     QRVWVDFAYGREPGSSDAIAWPRYRADARQALEFNSARSGLIDDYRREYCAFWNRYAI
                     L"
     gene            complement(2142..2642)
                     /locus_tag="PK818_07430"
     CDS             complement(2142..2642)
                     /locus_tag="PK818_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82597.1"
                     /translation="MTPRLLLVAALGLGLLPADARAGVALFCDDLYGQPINPRVDWQT
                     QVKPILNNDYGGRCTGCHNATSPAGFLDLTDNFMDAIYNIVGSVAFAGHPWDSILFEK
                     VNCENPPYGARMPRNQAPLTLAQQGLIYDWIAQGAYGENPPNAIQRDFIFRDGNESLR
                     LPYPGL"
     gene            2830..3468
                     /locus_tag="PK818_07435"
     CDS             2830..3468
                     /locus_tag="PK818_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82598.1"
                     /translation="MRPPFIVVLVFAALAPVASSRAFGWGGPAVEAVHGAQGATEPAA
                     CEDLSSVPVRHDIQYSMDDWPFGTDPASVTTILEDVLNVGGGCTGCHNGDALADGGQL
                     RLNQPSSVLRLVYAPSYRNFDILRVDPERPDLSLLYGMLNCEPPPTYPMMPPSMSGAR
                     IDIGLRAAVFDWIRAGARGRDQDGNLASDVLFRDQVESERFQQFLPEPPPTP"
     gene            3495..4085
                     /locus_tag="PK818_07440"
     CDS             3495..4085
                     /locus_tag="PK818_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82599.1"
                     /translation="MNRESPNPRSLHSPRRLRLAAGLLLACGLGANAATQAFPALAAP
                     GGGPGCDSLASEPVNYRVVFEDDIQPFFGGVLGSPINPRCETCHVTVTNGDMNLAPIN
                     VLVNLLGIDGDGQQSPNYPRRRIVPGKPEQSLLFLRINCDDAPDTGGRMPPGSAGGSE
                     DPEFLHFEALVHDWIKSGALMFGSDRVFQAGFDDLR"
     gene            complement(4362..>4766)
                     /locus_tag="PK818_07445"
     CDS             complement(4362..>4766)
                     /locus_tag="PK818_07445"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HQY82600.1"
                     /translation="VVTHTMSEFSRTINSNGNGTDHAWGQVAMVMGAPASGGGPLNGG
                     QVYGRYPLQVLGRQYPGAPDVYGECFNRGEFLPTTATEQMSGSLARWMGVSNTELPSI
                     FPNIDNFVNPHPNSAAMAYNARVIPNMMNGIS"
     assembly_gap    4767..4866
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4867..5759)
                     /locus_tag="PK818_07450"
     CDS             complement(<4867..5759)
                     /locus_tag="PK818_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HQY82601.1"
                     /translation="MSRIHFQKRRDFLKGATCMVASGAAASFVPQLSLMGTALAGTVT
                     GYKALVCIYLDGGNDSFNLLIPADNSTNIPVSGRQPLTGFSATPFGWYATSRGGYYAG
                     NPSSLAIPLVGGGMNGQLPNALPLNGNQHALNPAVPELQSLYNNGRLAFVANVGPLVF
                     PMRRSNFNSTARPPNLYSHNDQTSLWQIGAGDTASDPMGWGGKLAGALLGVSPASGLA
                     PCISIAGSTRYLTGEYPGGQTIAPYRLSTSATNPATSLNNYTGTQSADKRREVLTGLL
                     NEAYPQAFSNEYGEILDRSMNL"
     gene            complement(5770..>7654)
                     /locus_tag="PK818_07455"
     CDS             complement(5770..>7654)
                     /locus_tag="PK818_07455"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HQY82602.1"
                     /translation="VAWALGQIVITSDRDDFLANEPIMVAEWNDIIVRNALGNYRNLL
                     QEVTYSPMMGVYLTTLRNRKFELSQVGCPNGATSCALNNGVQPDENYAREVQQLFSIG
                     LLVRGPDFYTTYPDPGNPINLLNTYAEDDISTLARVFTGLSFSCTQGTSTVGGVPINT
                     NCGANNTACTGIGCRYTNAAGLFGRSPPGDPDPDDRGLLHPDWYRPMVCYPRYNDNGR
                     NVAGQVLPEPDSDPGLPAGSPPPNKVLVLNTSTGTVSLTIPPSFGPGSSTLNCHATGA
                     AVPADVQQQCIDYCEGSINQALDLLFNHPNTEVMVARQLIQRLVTSNPTPGYVTRVAQ
                     KFANNGAGVRGDMKATVRAVLMDAEARRPFAAANQPVNFGKVREPMLKQVALWRHFNA
                     ISGDTALLPATNPQGGNANPLAGQPGQRRWSTNNNPQDTYQQRPQGAPSVFNFYEPDY
                     RQPGAVTDAGLFSPEFQIIHEVTSVSAANDLYTRICSGYGGGNNNCSSGALTLPTDRA
                     YFPTAQVDLIPARMTLAGGAPAPGASPTLAEDTALIEFYNTRMMGGTMSGTIAANPQC
                     PSGTGTGMKGLLINALRCSGGVNADVTGGAAGAAGQVERQRRKALYLMHLIAISPEYS
                     TQR"
BASE COUNT         1063 a         2516 c         2756 g         1219 t
ORIGIN      
        1 gcctggtacg accccgccga tccgggctgg ggcgtgatgt cgcactactt ccgcggcggc
       61 gacggcgcca gccgggtatt ccgcacggtc tatttccacg actcggctgg cgcgccacgc
      121 tgggccttcg cacaggacac ggtggatggg ctgcgacagg cgcagacgta ctacacgccc
      181 tacgtggaat gcatcgactg tgcgatcgcg ccgatcctga ccacgccgat cggcagcggc
      241 accacgcggc tcacgcagcc gcttgcgcag gcggatgcgg cacgcaaccg catcgagctt
      301 gcgctgcgct tcgacagtgg cgcgcccttc gcacgcaaca cggctctcgc gctgatctcc
      361 gaacccctgc gcgtcgccgg tgccgcggcc accgcgcagg gaccgcttgc cggcagcgtg
      421 atcgacggcg ggatcgaatc gttcgtcgcg attccctatg ttgcgccgcc gctgggcgcg
      481 ctgcgctggc gcgcgccgca ggcgcccgcc ctgcgcgagc gcctgctcga agcgcgcgcg
      541 atcgggcccg ggtgccccca gcccgcgggc cagggcttct tcagcggcgc ggcggcgcgg
      601 catgacgagg attgcctgca gctcaacgtg tggcgtcccg ccacgcccgg gccgcatccg
      661 gtgatggtgt ggatccacgg cggcggactc acgcagggtt ccgcggtgca gttgcagaac
      721 ggcgtgctgc tgtacgacgg cgcggtgtac gcgcgccgcg acgtggtctt cgtctcgatc
      781 aactatcgcc tggggccgct gggcttcctc gcccagcgcg acttgcgcgg cgaggcgccc
      841 gaccatccgc agagcggcaa ctacgggttg ctggaccagg tcgccgcgct ggcctgggtc
      901 cgcgcgaaca tcgcggcctt cggcggcgat ccgcagcggg tcaccgtgta cggcgagtcg
      961 gcgggcgggg tcagcagttg cgtgctgctg gccacgccgg cggcgagcgg cctgttccag
     1021 cgcgcgatcg tgcagagcgg caactgcctg tggaacgcgc cgtcgctcga tgcagggatc
     1081 gagcagggcg atcgcgtgac gctcgcggcg ggctgcgtca ccgcgcccga ccgccgtgcc
     1141 tgcctgcgtg cgctcagcgt agcggcgctg ttcgcggccg ggccgccggt gatcagcacc
     1201 ggcgccagca ccgcgccggg cgaggtctac gggctggtgg tcgacggcta cgtactgccg
     1261 gaatcgccgg gtccggcgat tgccggcgga cgcgcggcgc cgctgccgct cctgatcggc
     1321 gtcaacgacg acgaacacgc gacgctcgca cccgcggcca gcctgccggc gacggcggct
     1381 ggctatgaag cggcggtgcg ctcgcgcttc ggcctgatcg gcggggaggt ggtcgcgcgc
     1441 tacccggccg cggcctatcc cacccccgcc ttggcctacc aggacctgct cgacgatgcg
     1501 cgcttcacct gcgccgcgcg ccgcgccggc gccgatcacg ccgcgcgcgg caacgcggtg
     1561 taccagtacg tgctgaccga gatcctgccc gatgccgggc tggtcgcgct ggagtccttc
     1621 catgcgctcg acgtgttgct gctgttcggc ccgcgcgccc aggcgcaggc gcccgaacgc
     1681 acgctggccg cgcgcatgca gcgcgtctgg gtcgatttcg cctacggccg cgagcccggc
     1741 agcagcgacg cgatcgcgtg gccgcgctac cgggccgacg cccgccaggc cctggagttc
     1801 aacagcgcgc gcagcggcct gatcgacgac taccggcgcg agtactgcgc cttctggaac
     1861 cggtacgcga tcctctgatt ccgttacggc gcgcgcccct gtaggagcgc atctcatgcg
     1921 cgaccggtgt cgcgatgtcg tccgaccagg cctcggcgcg gttcgcggtg ccgcggtcgc
     1981 gcatgagatg cgctcctgca cgcgcggata ggacaggccg ggggttcgtt gccaacgcgc
     2041 gcccctgtag gagcgcacct tgtgcgcgac cggtgtcgcg gtgtcgctcg atctggcccc
     2101 ggcgcggttc gcggtgcagc ggtcgcgcat gagatgcgct cctacagtcc cggataaggc
     2161 aggcgcaggg attcgttgcc gtcgcggaag atgaaatcgc gctggatggc gttgggcggg
     2221 ttctcgccgt aggcgccctg ggcgatccag tcgtagatca ggccctgctg cgccagggtc
     2281 agcggggcct ggttgcgcgg catgcgcgcg ccgtagggcg ggttctcgca attgaccttc
     2341 tcgaacagga tgctgtccca cggatggccc gcaaaggcca cgctgccgac gatgttgtag
     2401 atcgcatcca tgaaattgtc ggtgaggtcg aggaagccgg ccggcgaggt cgcgttgtgg
     2461 cagccggtgc agcgcccgcc gtagtcgttg ttgaggatcg gcttgacctg ggtctgccag
     2521 tcgacgcggg ggttgatggg ctgcccgtac aggtcgtcgc agaacagggc cacgccggcg
     2581 cgcgcatccg cgggcagcag gccgaggccc agcgcggcaa ccaggagcag gcgtggcgtc
     2641 atggcggcag gttagcgcag cgggttgggg agatcgcagc ctgcacgcag gtcgcggcag
     2701 ggatgcggca cgccttcacg gcgggaaaac aggctaggcg aagaccgcag ccgccatccg
     2761 ggcaaccggt cacaacatca cgatgtacgg aatcctaagg ttgcgggcat gccggtatcc
     2821 ttccccgcca tgcgtccgcc gttcatcgtc gtcctcgtct tcgcggccct cgcgcccgtc
     2881 gcgtcgtcgc gcgccttcgg ctggggcggg cctgccgtcg aggcagtcca tggcgcccag
     2941 ggcgcgaccg aaccggccgc ctgcgaagac ctgtcctcgg tgccggtgcg ccacgacatc
     3001 cagtactcga tggacgactg gcccttcggc accgatccgg cctcggtcac cacgatcctt
     3061 gaggacgtgc tcaatgtcgg cggcggctgc accggctgcc acaacggcga cgccctggcc
     3121 gatggcggcc aactgcgcct gaaccagccg tccagcgtgc tgcggctggt gtatgcgccc
     3181 agctaccgca acttcgacat cctgcgggtc gacccagagc gccccgacct cagcctgctg
     3241 tatggcatgc tcaactgcga gcccccgccg acctacccga tgatgccgcc ctcgatgagc
     3301 ggcgcgcgga tcgacatcgg gctgcgcgcg gcggtattcg actggatccg ggccggcgcg
     3361 cgcggccgcg accaggacgg caacctggcc agcgacgtgc tgttccgcga ccaggtcgag
     3421 tcggagcgct tccagcaatt cctgcccgag ccgccaccga cgccttgagt cccgcctgac
     3481 aagcacgcca gcgcatgaac cgcgaatccc cgaatcctcg atccctgcac tcgccgcgcc
     3541 gcctgcgtct cgcggcgggc ctgctgctgg cctgcggatt gggggcgaac gcggcgacgc
     3601 aggcgttccc ggccctggcg gcccccggcg gcggaccagg ctgcgattcc ctggcgagcg
     3661 agccggtcaa ctaccgggtc gtgttcgagg acgacatcca gccctttttc ggcggcgtgc
     3721 tgggttcgcc gatcaacccg cgctgcgaga cctgccacgt cacggtcacc aacggcgaca
     3781 tgaacctggc gccgatcaac gtgctggtga acctgctggg catcgacggc gacggacagc
     3841 agagcccgaa ctacccgcga cggcgaatcg tgccggggaa accggagcag agcttgctgt
     3901 tcctgcgcat caactgcgac gacgcgcccg acaccggcgg ccgcatgccg ccgggctcgg
     3961 ccgggggctc ggaggatccg gagttcctgc acttcgaggc gctggtgcac gactggatca
     4021 agtcgggggc cttgatgttc ggcagcgacc gggtgttcca ggcggggttc gacgacctgc
     4081 gttgagggcg ggcgcgatgc gcgttcgcgc tgtcgggatc gcggggctgc ctgatgggct
     4141 gcggtcgcgc acaaggtgcg ctcctacagg gccgcgcgtt gaactgccgg cgcttgtgca
     4201 ggagcgcatc tcatgcgcga ctgcggaacc acgtgccgcg gcgccttgcg ggtccgcggt
     4261 cgcgcatgag atgcgctcct acaacgacgt cgatacctga tcccgcaaac gaacacggcg
     4321 cccgcaggcg ccgtgatcga tcctgtgacg agaacccggc ctcagctgat accgttcatc
     4381 atgttcggga tgacccgggc gttgtaggcc atcgccgccg agttcgggtg cgggttcacg
     4441 aagttgtcga tgttggggaa gatgcttggc aactcggtgt tgctcacgcc catccagcgc
     4501 gccagcgagc ccgacatctg ctcggtcgcg gtggtgggca ggaactcgcc gcggttgaag
     4561 cattcgccgt agacgtccgg cgcgcccgga tactggcggc ccagcacctg cagcggatag
     4621 cggccgtaga cctggccgcc gttgagcggg ccgccgccgg aagccggcgc gcccatcacc
     4681 atcgccacct ggccccaggc atggtcggtg ccgttgccgt tggagttgat ggtgcgcgag
     4741 aactcgctca tggtgtgggt cacgacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnaggt tcatcgagcg gtcgaggatc tcgccgtact cgttgctgaa ggcctgcggg
     4921 taggcttcgt tgagcaggcc agtcaacacc tcgcggcgct tgtcagccga ctgcgttccc
     4981 gtgtagttgt tgagcgaggt cgcgggattg gtcgcgctgg tcgagagccg gtaaggtgcg
     5041 atggtctggc cgcccgggta ctcgccggtc aggtagcggg tggagcccgc gatcgagatg
     5101 cagggcgcca ggccgctcgc cggggaaacg cccagcagcg cgccggccag cttgccgccc
     5161 caacccatcg ggtcgctggc ggtgtcgccg gcgccgatct gccacaggct ggtctggtcg
     5221 ttgtgcgaat agaggttcgg cggccgcgcg gtgctgttga agttgctgcg ccgcatcggg
     5281 aacaccagcg ggccgacatt ggccacgaag gccaggcgcc cgttgttgta cagcgactgc
     5341 agttccggca ccgcggggtt cagcgcatgc tggttgccgt tgagcggcag cgcgttcggc
     5401 agctggccgt tcatgccgcc gccaaccagc gggatcgcca gcgacgaagg attgccggcg
     5461 tagtagccgc cgcgcgaagt cgcgtaccag ccgaagggcg tggcgctgaa gcccgtcagg
     5521 ggctggcgcc cgctgaccgg aatgttggtg ctgttgtcgg cgggaatcag caagttgaag
     5581 gaatcgttgc cgccgtcgag gtagatgcac accagcgcct tgtaaccggt cacggtgccg
     5641 gccagcgcgg tgcccatcag cgagagctgc ggcacgaagg aggcggcggc gccggaggcg
     5701 accatgcagg tcgcgccctt gaggaagtcg cggcgcttct ggaagtggat gcgggacatg
     5761 tcgttctcct cagcgctgcg tgctgtattc gggcgagatc gcgatcaggt gcatgaggta
     5821 cagggccttg cggcgctgac gctcgacctg gccggccgcg cccgcggcgc cgcccgtgac
     5881 atccgcattg acgccgccgg agcagcgcag cgcgttgatc agcaggccct tcatgccggt
     5941 gccggtgccc gacgggcact gcggattcgc ggcgatcgtg cccgacatcg tgccgcccat
     6001 catgcgcgtg ttatagaact cgatcagcgc cgtgtcctcg gccaacgtgg ggcttgcgcc
     6061 cggggccggc gcgccgccgg ccagggtcat ccgtgccggg atgaggtcca cctgcgcggt
     6121 cgggaagtag gcccgatcgg tcggcaaggt gagggcaccg gacgagcagt tgttgttgcc
     6181 gccgccgtag cccgagcaga tgcgcgtgta gagatcgttg gccgcgctga ccgaggtcac
     6241 ctcgtggatg atctggaact cgggcgagaa caggcccgcg tcggtcaccg cgccaggctg
     6301 gcggtagtcg ggttcataga agttgaacac cgagggcgcg ccctgcggcc gctgctggta
     6361 ggtgtcctgc gggttgttgt tggtgctcca gcggcgctgg ccgggctgac cggcgagcgg
     6421 gttggcattg ccaccctgcg ggttggtcgc gggcaggagc gcggtgtcgc cggagatggc
     6481 attgaaatgc cgccacaggg cgacctgctt gagcatcggc tcgcgcacct tgccgaagtt
     6541 gaccggctgg ttggcggcgg caaagggacg ccgggcctcg gcatccatca gcaccgcgcg
     6601 caccgtggcc ttcatgtcgc cgcgcacgcc ggcgccgttg ttggcgaact tctgcgccac
     6661 gcgcgtcaca tagcccggcg tggggttcga ggtcacgagg cgctggatca gctggcgcgc
     6721 caccatcact tcggtgttgg ggtggttgaa cagcaggtcg agggcctggt tgatgctgcc
     6781 ctcgcagtag tcgatgcact gctgctgcac gtcggccggc acggcggcgc cggtcgcgtg
     6841 gcaattgagc gtcgacgaac ccggcccgaa gctgggcggg atcgtcagcg acacggtgcc
     6901 ggtgctggtg ttgaggacca gcaccttgtt gggcggcggc gagccggccg gcaggcccgg
     6961 atcgctgtcg ggctccggca ggacctggcc ggccacattg cggccgttgt cgttgtagcg
     7021 cgggtagcag accatcgggc ggtaccagtc cgggtgcagc aggccgcggt cgtccgggtc
     7081 cggatcgccc ggggggctgc ggccgaaaag gccagccgcg ttggtgtaac ggcagccgat
     7141 gcccgtgcac gcggtgttgt tggcgccgca gttggtgttg atcggcacgc cgccgacggt
     7201 gctggtgccc tgggtgcagg agaaggacaa gccggtgaac acgcgcgcca gggtgctgat
     7261 gtcgtcctcg gcgtaggtgt tgagcaggtt gatcgggtta cccggatcgg ggtacgtggt
     7321 gtagaagtcc gggccgcgca ccagcaggcc gatcgagaac agctgctgca cttcgcgcgc
     7381 gtagttctcg tcgggctgca cgccgttgtt gagcgcgcac gaggttgcgc cgttcgggca
     7441 acccacctgc gacagctcga acttgcggtt gcgcagggtg gtcaggtaca cgcccatcat
     7501 cgggctgtag gtgacttcct gcagcaggtt gcggtagttg cccagcgcgt tgcggacgat
     7561 gatgtcgttc cactccgcca ccatgatcgg ctcgttggcg aggaagtcgt cgcgatcgga
     7621 ggtgatcacg atctggccca gggcccaggc cacg
//
LOCUS       DAORUK010000098         7652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_10749_length_7652_cov_17.327103, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000098 DAORUK010000000
VERSION     DAORUK010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10749_length_7652_cov_17.327103"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /gene="serS"
                     /locus_tag="PK818_07460"
     CDS             complement(<1..1000)
                     /gene="serS"
                     /locus_tag="PK818_07460"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HQY82603.1"
                     /translation="MLDPALLRANPAELAERLRSTRGFELDVTHLESLEVQRKLLQVR
                     TQELQNLRNTRSKQIGMLKAKGEDVSAVMAEVAGFGEELKASEIRLDELRGEIEAIAL
                     GIPNLPDASVPPGGDETDNVEQKTWGTPKQFDFAVKDHVELGARHGWLDGETAAKLSG
                     SRFTVLRGQLARLHRALAQFMLDLHTGEHGYEETNVPVIVNAESMRGTGQLPKFEEDL
                     FSTQLGEHKRYLIPTSEVPLTNIVRDEILDAERLPLRMTAHSMCFRAEAGAAGRDTRG
                     MIRQHQFEKVELVSIAKPEDSDAEHQRMTRCAETVLEKLGLPYRKVLLCTGDMGFGA"
     gene            1101..1730
                     /locus_tag="PK818_07465"
     CDS             1101..1730
                     /locus_tag="PK818_07465"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQY82604.1"
                     /translation="MPDPTPRRARLLPPHIAKPLAIAFGIGLLLFLLLWLDQRNDSDF
                     FKAGTPASGTNGDPEALPAPVPADIASEDGGASGLGLPPGDTRPAPRTSEAPRIIEPP
                     APPPAPPAPTAPAPIADSDAPVPVSRPPPRYPPEALRRNIGGSVRVQATVAADGSVER
                     LDLAQSSGNRDLDRAALEAVRRWRFQPAQRNGQPVSATVIIPLEFNPGG"
     gene            1886..2458
                     /locus_tag="PK818_07470"
     CDS             1886..2458
                     /locus_tag="PK818_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQY82605.1"
                     /translation="MSTLDAPSREPKDWSADMAAVALHRDRDCFLRIYDHFMPRVCQY
                     LRGMGAPDAVAEELAQEAMLRLWQHAAGYDPARSAVSTWLFRIARNLHIDRIRREPAT
                     HQGLGQEVEFDVELQPDPASAEDYAESVHLKRRIDALSSVQARLIRMSYFEAKTHQEI
                     AGELSMPLGTVKSHLRRAFLRLQGEIGGQP"
     gene            2455..3120
                     /locus_tag="PK818_07475"
     CDS             2455..3120
                     /locus_tag="PK818_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ChrR family anti-sigma-E factor"
                     /protein_id="HQY82606.1"
                     /translation="MNPRHHLDPATVLSHAAGALLPELAAIVATHLEGCAACRDAVAR
                     AERVGGQLIEQQHPQPALEDRARQLRAAMLERLDATPPAPVRASQPQGRDPDRIPAPL
                     QPYFGASYRELKWRWMGPGMHFIRRPGPSGASLLMLRIAPGKRMPVHSHGGSEITQIL
                     SGAYDDALGHFAAGDVADLDSEIEHQPVTTAGVPCICVSALDAPLRFPGWLARRLQPV
                     FGI"
     gene            3188..3964
                     /locus_tag="PK818_07480"
     CDS             3188..3964
                     /locus_tag="PK818_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82607.1"
                     /translation="MTAPLPALACFVALVFASMAASAADVRRDEARVLAAGDGRLLYR
                     ETHWTLPGPRPDRWVLYRCADGAAFARKRVLAAPSPLAPDFALEDARDGYREGVRAAA
                     GGRSVFVHAPGRAEATRVLAIPADGVIDAGFDAAVRRHWPRLVRGEAVKLQFLVPSRK
                     RFYPVQVQRVASVDWNGVPAERLRMRLDAWFGFAVPDVTLLYARDDQRLLEFAGTGNL
                     RDGDGDHPQVRIVFERTPRTATAQELADARSLPLAGRCRF"
     gene            4046..4999
                     /locus_tag="PK818_07485"
     CDS             4046..4999
                     /locus_tag="PK818_07485"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HQY82608.1"
                     /translation="MSRNPPPRQPPTAQPRLAALRQWFDSDAAPAPDRGGDDRIDWLR
                     ALPFIGMHLACLGVLWVGVSATALWVAVALYALRMFAITGFYHRYFSHRTFRTSRVVQ
                     FAFALLGAASVQRGPLWWAAHHRHHHRHADTAADPHSPRKGFWRSHAGWFLTRGGFRT
                     DPSRIGDLIRYPELRWLDRFDTAVPVALAVLLFLAGMALERWAPGLGTNGPQLLVWGF
                     FVSTVVLFHATVTINSLAHRWGKRRFDTRDDSRNNLWLALITFGEGWHNNHHFFPGSA
                     RQGFRWWEIDLTWYGLRAMQALGLVRDLKPVPAWVLAKVEN"
     gene            5001..6305
                     /locus_tag="PK818_07490"
     CDS             5001..6305
                     /locus_tag="PK818_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY82609.1"
                     /translation="MRIAVVGSGISGLSAALLLSRKHEVVLFEGNDYLGGHTDTHRVE
                     VDGRMLEVDTGFIVHNPQHYPLLTRLFEELGVATQATTMGFSVQDAGSGLEYNATSLD
                     ALFCQRRNLASPRFLGMLRDLVRFYRNAPELLAGDGPGPALGDYLQAKGYGDAFRDQH
                     LVPMASALWSSPAVDILRFPARYLVQFMANHHMLQLAGRPEWRVVRGGSRRYVEAMTA
                     RWDVQVRLRSPVRAIARDADGAAVRCDAGSERFDQVVLACHSDQALALLEDASYLERD
                     ILGAITYQHNDTVLHTDARLLPRNRKAWAAWNAYLPADPAQACTVSYCMNLLQGLPGE
                     TPLVVTLNRTGDIDPARILVRRGYQHPVYTHAAVAAQARRHEIQGTRHTWFAGAYWGW
                     GFHEDGIRSGVEVARALGVAWPGVAATATPPAKRVRSMAAAA"
     gene            6311..7072
                     /locus_tag="PK818_07495"
     CDS             6311..7072
                     /locus_tag="PK818_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HQY82610.1"
                     /translation="MASAVYEGTVRHRRHAPCAHAFGYRMAQLYLDLDEVDTVFRDRW
                     LWSVERRNLAAFHRRDYLGDPAQPLAEAVRDRVQAATGERPPGPVRLLTHLRYAGLSF
                     NPVSFYYCFDATGALHSIVAEITNTPWKERHAYVLRVADAGRRGRMLHWGFDKAFHVS
                     PFLPMDRRYGWAFSVPGDELRVHMDVCDGDTREFDADLHLQRRPLDGRSLARVLWRYP
                     LMTAQVVAGIHWQALRLWLKRVPVHDHPSRLESTR"
     gene            7069..>7652
                     /locus_tag="PK818_07500"
     CDS             7069..>7652
                     /locus_tag="PK818_07500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY82611.1"
                     /translation="MNAIVHTAPANAYTGLDRFLRTRLLRQLDALEGRAVELHDALGS
                     ECVGRGDAPPLRLRVHGPGFFRALAGNGSVGAAEAFMDGDWDCDDLVALVQLLVRNRD
                     LLDAMERGPARLGGLAMRGWNALRRNTRDGARRNIAAHYDLGNAFFQLFLSDDLMYSS
                     ALYAGEDEPLDIASTRKLDRICDKLRLGPDDHVV"
BASE COUNT         1066 a         2820 c         2623 g         1143 t
ORIGIN      
        1 tcgcgccgaa gcccatgtcg ccggtgcaca gcagcacctt gcggtacggc aggcccagct
       61 tttccagcac ggtttcggcg cagcgggtca tgcgctggtg ctcggcgtcg gagtcttccg
      121 gcttcgcaat gctgaccagc tcgacctttt cgaactggtg ctggcggatc atcccgcggg
      181 tgtcgcggcc ggccgcgcca gcctcggcgc ggaagcacat cgaatgcgcg gtcatccgca
      241 gcggcagccg ctcggcgtcg aggatctcgt cgcgcacgat gttggtcagc ggcacttcgc
      301 tggtcgggat caggtagcgc ttgtgctcgc ccagctgggt ggaaaacagg tcttcctcga
      361 acttcggcag ctggccggtg ccgcgcatcg actcggcgtt gacgatcacc gggacgttgg
      421 tttcctcgta gccgtgctcg ccggtgtgca ggtccagcat gaactgcgcc agcgcgcggt
      481 gcaggcgggc cagctggccg cgcagcacgg tgaagcgcga gccgctcagc ttggccgcgg
      541 tttcgccatc cagccagccg tggcgggcgc cgagttcgac gtggtccttg accgcgaaat
      601 cgaactgctt cggggtgccc caggtctttt gctcgacgtt gtcggtttcg tcgccgcccg
      661 ggggtaccga ggcgtcgggg agattcggga tgcccagcgc aatcgcctcg atctcgccgc
      721 ggagttcgtc caggcggatt tccgaagcct tcaactcctc gccgaagccg gcgacttccg
      781 ccatcaccgc ggacacgtcc tcgcccttgg ccttgagcat gccgatctgc ttgctgcggg
      841 tgttgcgcag gttctgcagt tcctgggtac ggacctgcag cagcttgcgc tggacctcca
      901 gcgattccag gtgggtgacg tcgagttcga agccgcgggt cgagcgcagg cgttcggcaa
      961 gctcggcggg gttggcgcgc agcagggcag gatcaagcat ggacggggct tgcagggtga
     1021 agacttgcca ttatcgccga tgcgccggcg gatcgccgcg gcgtgtcgaa accctcgcat
     1081 cgccatgcca gaatgcggcc atgcccgacc ccacgccccg gcgcgcacgc ctgctgccgc
     1141 cgcacatcgc gaagccgctg gcgatcgcct tcggcatcgg cctgttgctg ttcctgctgc
     1201 tgtggctgga ccagcgcaac gacagcgact tcttcaaggc cggcaccccc gccagcggca
     1261 ccaacggcga tccggaagcc ttgccggcgc cggtacccgc ggacatcgcc agcgaggatg
     1321 gcggcgccag cggcctgggc ctgccacccg gggacacccg cccggcgccg cgcaccagcg
     1381 aggcgccaag gatcatcgag cctccggcgc cacccccggc accacccgca ccgacggcgc
     1441 cggccccgat cgcggacagc gacgcaccgg tgccggtcag ccggccgccc ccgcgctacc
     1501 cgccggaagc cctgcgccgc aacatcggcg gcagcgtgcg ggtgcaggcc acggtggcgg
     1561 ctgacggcag cgtggagcgc ctggacctgg cgcaaagcag cggcaaccgc gacctcgacc
     1621 gcgccgcgct ggaggcggtg cggcgctggc gcttccagcc cgcccagcgc aacggccagc
     1681 cggtgagcgc gacggtgatc atcccgctgg agttcaatcc cggcggttga acaacgggca
     1741 ggatccggtc ctggaggaat ccacccggcg ctgcatccgg atccgcacct gctgcgtaca
     1801 agccggcgaa tccaatggaa cgccgtgtac gatggccagc ccccgccccc tggagaaacc
     1861 gtcgcccttc gaggcggcca gacgcatgtc caccctggac gcgccctccc gcgagccgaa
     1921 ggactggtcc gccgacatgg ccgcggtcgc gctgcaccgt gaccgcgact gcttcctgcg
     1981 catctacgac cacttcatgc cgcgcgtctg ccagtacctg cgcggcatgg gcgcgcccga
     2041 cgcagtggcc gaggaactgg cgcaggaggc gatgctgcgc ctttggcagc acgccgcggg
     2101 ctacgacccg gcgcgcagcg cggtcagcac ctggctgttc cggatcgcgc gcaacctgca
     2161 catcgaccgc atccgccggg aacccgccac ccaccaaggc ctgggacagg aggtcgagtt
     2221 cgacgtcgaa ctgcagcccg accccgccag cgccgaggac tacgccgaat ccgtccacct
     2281 caagcgccgc atcgacgccc tttcgtcggt gcaggcacgg ttgatccgca tgtcgtactt
     2341 cgaagccaag acccaccagg aaatcgccgg cgagctctcg atgccgctgg gcaccgtcaa
     2401 gtcgcacctg cgccgcgcgt tcctgcgcct gcagggcgag atcggggggc agccatgaat
     2461 ccgcgccacc acctcgaccc ggccaccgtg ctcagccacg cggcgggcgc gctgctgccg
     2521 gaactggcgg cgatcgtcgc cacccacctg gaaggctgcg ccgcctgccg cgatgcggtg
     2581 gcgcgcgcgg agcgcgtcgg cggccagctg atcgaacagc agcacccgca gccggcgctg
     2641 gaggaccgtg cccggcagtt gcgcgcggcg atgctggaac gcctggatgc caccccgccg
     2701 gcgcccgtgc gcgccagcca gccgcagggc cgcgatcccg accgcatccc ggcgccgctg
     2761 cagccgtatt tcggcgcctc ctaccgcgag ctcaagtggc gctggatggg acccggcatg
     2821 catttcatcc gccgccccgg ccccagcggc gccagcctgc tgatgctgcg gatcgcgccg
     2881 ggcaagcgga tgccggtgca cagccatggc ggcagcgaga tcacccagat cctgtccggc
     2941 gcctacgacg acgccctggg ccacttcgcc gccggcgacg tcgccgacct cgacagcgag
     3001 atcgagcacc agccggtcac caccgccggg gtgccctgca tctgcgtgtc ggcgctggat
     3061 gcaccgctgc gctttcctgg ctggctggcc cgccgcttgc agccggtgtt cgggatctga
     3121 ccctgctgca tccaccgcga cggcctggcc gtctctgctg gcaaccatcc gcgcaccgga
     3181 atccgtcgtg actgcaccgc tgcccgcgct cgcctgcttc gtcgccctgg tgttcgccag
     3241 catggcggcc agcgccgccg acgtgcgtcg cgacgaggcc agggtgctgg cagcaggcga
     3301 cggccgcctg ctgtaccggg aaacccactg gaccctgccc ggcccgcggc cggaccgctg
     3361 ggtgctgtat cgctgcgcgg atggcgcggc gttcgcccgc aagcgggtgc tggccgcgcc
     3421 ctcgccgctg gcgcccgact tcgcgctgga agacgcgcgc gatggctacc gcgaaggcgt
     3481 gcgcgccgcg gccggcggac gcagcgtgtt cgtgcacgcg cccggccggg cggaggcgac
     3541 gcgggtgctg gcgatccccg ccgatggcgt gatcgatgcc ggattcgacg ccgcggtgcg
     3601 caggcactgg ccgcgactgg tgcgcggcga agcggtgaaa ctgcagttcc tggtgcccag
     3661 ccgcaagcgc ttctacccgg tgcaggtgca gcgtgtcgcc agcgtcgact ggaacggcgt
     3721 gcctgccgaa cgcctgcgca tgcggctgga tgcgtggttc gggttcgcgg tgccggacgt
     3781 gaccctgctg tacgcacgcg acgaccagcg cctgctcgaa ttcgccggca ccggcaacct
     3841 gcgcgatggc gatggcgacc acccgcaggt gcggatcgtg ttcgagcgca cgccacgcac
     3901 ggccaccgcg caggagctcg ccgatgcccg cagcctgccg ctggcgggac gctgccgctt
     3961 ctgatcgccc gcagccgcgg cgtcccgcgc ctgcatccgg attcgccgcc gcgccgtatg
     4021 cagtggcagg aaacaggagc cccggatgtc gcgcaaccct cccccacgcc agccaccgac
     4081 cgcgcagccg cggctggccg cgttgcgcca gtggttcgac agtgatgccg cgcccgcgcc
     4141 cgaccgcggc ggcgacgacc gcatcgactg gctgcgcgcg ctgccgttca tcggcatgca
     4201 cctggcctgc cttggcgtgc tgtgggtcgg ggtgtccgcg accgcgctgt gggtggcggt
     4261 ggcgctgtat gcgctgcgca tgttcgcgat caccggcttc taccaccgct acttctcgca
     4321 tcgcacgttc cgcacttcgc gcgttgtcca gttcgcgttc gcgctgctgg gcgcggcttc
     4381 ggtgcagcgc gggccgttgt ggtgggccgc ccaccatcgc caccaccacc ggcatgcgga
     4441 taccgcggcc gacccgcaca gcccgcgcaa gggcttctgg cgcagccacg ccggctggtt
     4501 cctgacccgc ggcggcttcc gcaccgatcc ttcgcgcatc ggcgacctca tccggtaccc
     4561 ggaactgcgc tggctggacc gcttcgatac cgccgtgccg gtcgcgctgg ccgtgctgct
     4621 gttcctggcc ggcatggccc tcgagcgctg ggcgcccggc cttggcacca atggcccgca
     4681 gttgctggtg tggggcttct tcgtctccac cgtggtcctg ttccacgcca ccgtcaccat
     4741 caactcgctg gcccaccgct ggggcaagcg ccgcttcgac acccgcgacg acagccgcaa
     4801 caacctgtgg ctggcgctga tcaccttcgg cgagggctgg cacaacaacc accacttctt
     4861 cccgggcagc gcgcgccagg gcttccgctg gtgggagatc gacctgacct ggtacggcct
     4921 gcgcgcgatg caggcgctgg ggctggtgcg cgacctcaag ccggtgcccg cctgggtact
     4981 ggcgaaggtg gagaactgag atgcggatcg cggtcgtcgg gtcgggcatc agcgggctgt
     5041 cggcggcgct gctgctgtcg cgcaagcatg aggtggtgct gttcgagggc aacgactacc
     5101 tcggcggcca caccgatacc catcgggtcg aggtcgacgg gcggatgctg gaggtggaca
     5161 ccggcttcat cgtccacaac ccgcagcact acccgctgct gacccgcctg ttcgaagaac
     5221 ttggcgtcgc cacccaggcg accaccatgg gcttctcggt gcaggacgcc ggcagcggcc
     5281 tggaatacaa cgcgacctcg ctggatgcgc tgttctgcca gcgccgcaac ctcgcctcgc
     5341 cgcggttcct gggcatgctg cgcgacctgg tccgcttcta ccgcaacgca cccgaactgc
     5401 tcgcgggcga cggcccgggc ccggcgctcg gcgactacct gcaagccaag ggttacggcg
     5461 atgccttccg cgaccagcac ctggtgccga tggcgagcgc gctgtggtct tcgccggcgg
     5521 tcgacatcct gcgtttcccg gcccgttacc tggtccagtt catggccaac caccacatgc
     5581 tgcagctggc cgggcggccc gaatggcggg tggtgcgcgg cggctcgcgg cgctacgtgg
     5641 aggcgatgac cgcgcgctgg gacgtgcagg tgcggctgcg ctcgccggtg cgcgcgatcg
     5701 cccgcgatgc cgatggcgcg gccgtgcgct gcgatgccgg cagcgagcgc ttcgaccagg
     5761 tcgtgctggc ctgccacagc gaccaggcgc tggcgctgct cgaggatgcg agctatctgg
     5821 aacgcgacat cctcggcgcg atcacctacc agcacaacga caccgtgctg cacaccgacg
     5881 cacgcctgct gccgcgcaac cgcaaggcgt gggccgcctg gaacgcctac ctgccggcgg
     5941 atcccgcgca ggcctgcacc gtgagctact gcatgaacct gctgcagggc ctgcccggcg
     6001 agacgccgct ggtggtgacc ctgaaccgca ccggcgacat cgacccggcg cgcatcctgg
     6061 tccgccgcgg ctaccagcat ccggtctaca cccacgccgc ggtcgccgcg caggcacgcc
     6121 ggcacgagat ccagggcacc cgccacacct ggttcgccgg tgcctactgg ggttggggtt
     6181 tccacgaaga cggcatccgc agcggggtgg aagtcgcgcg cgcgctcggc gtggcatggc
     6241 ccggcgtggc cgccacggca acgccgccag caaagcgcgt gcgaagcatg gcggcagccg
     6301 cgtgaccagc atcgccagtg cggtctatga aggcaccgtg cggcatcgcc ggcatgcgcc
     6361 gtgcgcgcac gcgttcggtt accggatggc gcagctttac ctggacctcg acgaagtgga
     6421 caccgtgttc cgcgaccgct ggctgtggtc ggtggagcgc cgcaacctgg ccgcgttcca
     6481 ccgccgcgat tacctcggcg atcccgcgca accgctggcc gaggcggtgc gcgaccgcgt
     6541 gcaggccgcc accggcgagc gtccgccggg cccggtgcgc ctgctcaccc acctgcgcta
     6601 cgccggcctc agcttcaacc cggtcagctt ctactactgc ttcgatgcca ccggcgcgct
     6661 gcacagcatc gtcgccgaaa tcaccaacac gccgtggaag gagcgccacg cttacgtgct
     6721 gcgggttgcc gacgccgggc gccgcggccg catgctgcat tggggcttcg acaaggcctt
     6781 ccacgtgtcc ccgttcctgc cgatggaccg gcgctacggc tgggccttca gcgttcctgg
     6841 cgacgagctg cgcgtgcaca tggacgtgtg cgacggcgac acccgcgagt tcgatgccga
     6901 cctgcacctg cagcggcgac cgctggacgg acgcagcctg gcacgcgtgc tctggcgcta
     6961 cccgctgatg accgcgcagg tggtcgccgg catccactgg caggcgctgc ggctgtggtt
     7021 gaagcgcgtg cccgtccacg accacccttc acgactggaa tcgacgcgat gaacgccatc
     7081 gtccacaccg cgcccgccaa cgcctacacc ggcctcgacc gcttcctgcg cacccgcctg
     7141 ctgcgccagc tcgatgcctt ggagggccgc gcagtggaac tgcacgacgc gctgggcagc
     7201 gaatgcgtgg gccgcggtga cgcgccgccg ctgcgcctgc gcgtgcatgg ccccgggttc
     7261 ttccgcgcgc tggccggcaa cggcagcgtg ggcgccgcgg aggcgttcat ggacggcgac
     7321 tgggactgcg acgacctggt cgcgctggtg caactgctgg tgcgcaaccg cgacctgctg
     7381 gatgcgatgg agcgcggccc ggcgcgcctg ggcgggctgg cgatgcgcgg ctggaatgcc
     7441 ctgcgccgca acacccgcga cggcgcacgc cgcaatatcg ctgcgcacta cgacctcggc
     7501 aacgcgttct tccagctgtt cctgtcggac gacctgatgt actcgtccgc gctgtatgcc
     7561 ggcgaggacg aaccgctgga catcgcctcc acccgcaagc tggatcgcat ctgcgacaag
     7621 ttgcggctgg ggccggacga ccacgtggtc ga
//
LOCUS       DAORUK010000099         7533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_10998_length_7533_cov_19.301284, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000099 DAORUK010000000
VERSION     DAORUK010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7533
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10998_length_7533_cov_19.301284"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PK818_07505"
     CDS             complement(<1..1006)
                     /locus_tag="PK818_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017167545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HQY82612.1"
                     /translation="MQAPRSVLRVALPLPLPRTFDYLPPPGVAAATVAAGQRIVVPFA
                     GRELCGIVVGHGDPEPGIEPKPALALPEALPLLQGELLASLQWLAGYLHAPLGDVLAT
                     ALPAVLRRGEPLADTTRHGWRLAEAGCTALPAMRAGRPQALAALLADVRDEAWLDAEH
                     AGWRAPMRGLRERGLVERVALHQERGPFPAAPTQPAPVLNPEQRSAADAIRAADGFAS
                     FLLDGVTGSGKTEVYLDAIGDCLARGRQALVLVPEIGLTPQALARFRARLGVPVHALH
                     SGLNDNERARVWLAAARGQARVIVGTRSAVFVPLPEAGLIVVDEEHDGSYKQFDGIRY
                     H"
     gene            1473..2867
                     /locus_tag="PK818_07510"
     CDS             1473..2867
                     /locus_tag="PK818_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82613.1"
                     /translation="MTTAPGSPSPAAVVAFLRGIDRRARLFATVQAGDPARGARALAV
                     VARVFAAEAGQWPLAQWPQQYWRLLLATPSLRQAAAPDGQVVLAGIARLPADARAGVL
                     LQLVAGLGDADAALALGLPLATYQARIRDSLPRDPLGQPDVDVWRAWRAAAQRELELG
                     NAAVAQDAPAPPTTGPRRVRAATVPELPAPHGVRWLWLGVAGCVLAFAAAFFIHPAGR
                     EAIHQWFATIKREPLPPAAAAKARFDAGDIALHPDRERLAAPREAWFADRLALLAWLA
                     NAEAGPAADPAAAEVLPLPGAAPAAPPSLPDAGRQATQLAQRMRAWDALPPRARGLQR
                     GHWQAWQALEAGERVRLRSLGERLRMSPAPLQQALRERFDAQTSDARAGWWLGPRLGR
                     DWPRVAALFGYVEAAERGPLLDFLRTAGAEDIDACARLAQTTPPEQRAALRAQLLAQP
                     AAQRGAWLQARLQR"
     gene            complement(2881..4056)
                     /locus_tag="PK818_07515"
     CDS             complement(2881..4056)
                     /locus_tag="PK818_07515"
                     /inference="COORDINATES: protein motif:HMM:NF023828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HQY82614.1"
                     /translation="MTEPTATNAPAAPDPAPAGTPCGNCGAPLLGDHCYACGQPVKGL
                     VRHFSSILGDALDSVFNLDTRIFRTIGPLFARPGHITREYFAGRRVRYVSPVRLFFFL
                     AIVTFFVAQLTVDLGADAIRMDGKRSNNPISSAMTVAEAEKARDAELAEMATAKAGMA
                     GTPAAVGIPGLEAGERAVRDTASARIRQLQEAAAKGQPPPEPMQDNFNISLGGERWDA
                     KKPVDTWLPGFADRWLHQQVTRANDNVRRLKQDPGAFKNAALGAVPTTLFVLVPVFAL
                     MLKLIYLFKRRLYMEHLIVGLHSHAFLCLDLLLVLLLHALSRAYTADGSLANDLFDWV
                     EGLLVAWMPLYLLLMQKRVYAQGWPMTVLKFGVLGTCYSILLSFAIVASMGIGLVAM"
     gene            4132..5538
                     /locus_tag="PK818_07520"
     CDS             4132..5538
                     /locus_tag="PK818_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012481373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQY82615.1"
                     /translation="MNPSADSPRFADEVRQSARLAAPLAAGHLSHGLVAFVDTVVAGR
                     HGTTTLAAVAVGTALFWLPMMLPMGTLMALPPSVSQLDGARRRGEIGPLWRQSLWLAL
                     GLGALLFALVTVLPLMLGPMGIAADIIPGATGFLHAIRWGIPAFTVYLCMRYLSDGLH
                     WSLPTMLIGFGGLLLLAPGGYVLTNGLFGLPEMGAAGLGWASAAMFWGQLLAFALYLR
                     LSPRFADLGLYAHWERPQWATIRGLLGRGLPIGVTITMEGSLFIVTALLIGRLGEVPV
                     AAHQIAINVASLCFMIPFGVAEATTVRVGHALGRGDRDGIRRAYFAGLALVLGTQALS
                     ALVMLLGNHAIVGLYTGDAVVGALAASLLLYAALFQFPDGVQVLSAGALRGLNDTRVP
                     MWLAALAYWGIGMPVGAGLGLALGWGPRGMWLGLTAGLSVAAVLLARRFLRSSLRVPI
                     VLQARIEGGSSVTVTDAA"
     gene            5641..>7533
                     /gene="sppA"
                     /locus_tag="PK818_07525"
     CDS             5641..>7533
                     /gene="sppA"
                     /locus_tag="PK818_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HQY82616.1"
                     /translation="MNQPRSRGPIARVFIGLWDVLNFSRRLVLNLLFLLVLFVLLAAM
                     LGGGKLAPLAERSTLVIEPQGRLVEQYSCDPVSRAFARATNGDDCREIRLRDLLRALE
                     SARTDKRIERVLLRLDELQPGGFASLREVAAAIARVKAAGKPVVAHGDNYSQGQYLLA
                     AHASKVYLDPMSQGGVMLEGLAGYRQYFREGLQDKLGVDVHLFKVGEYKSAAEPYILD
                     AASPESKEADLFWMNDVWQRMLADIGKARGLPPATLAADIDAMAEQVAAAKGDLAALA
                     LQQKLVDGLKTREEVEELLADEGEADDNAEGGFRQVALDTYVQHLDGALQPADMRPQV
                     AVVVAEGAIAGGELPPGEVGGESTSALLRAARDDDRVKALVLRVNSPGGEVFASEQIR
                     REVAALKQAGKPVVVSMGDVAASGGYWISMNADRIYADPSTITGSIGIFGLFPTADRA
                     LAKLGVRTDGVGTTRYAGAFDLTRPLDPGVAAVVQSVIDKGYRDFTGKVAQARGRSVA
                     QIDEVARGRVWSGEQARQRGLVDALGGLDAAVADAAARAKLGKPSTWHTHYLEETAKP
                     FEQWFGGLVGSGAGIGLLRATGVGEALLLEHVASQAAAPLRVVQNALKQRGNVRVTPL
                     AHCFCSA"
BASE COUNT         1016 a         2613 c         2795 g         1109 t
ORIGIN      
        1 cgtggtagcg gatgccgtcg aactgcttgt agctgccgtc gtgttcctcg tcgaccacga
       61 tcaggccggc ctccggcagc ggcacgaaca ccgccgagcg cgtgccgacg atcacccgcg
      121 cctgcccgcg cgcagcggcc agccagaccc gcgcgcgttc gttgtcgttc aagccggaat
      181 gcagcgcatg caccggcacg ccgaggcggg cgcggaagcg cgccagcgcc tgcggggtca
      241 ggccgatctc cggcaccagc accagcgcct gcctgccgcg ggccaggcag tcgccgatcg
      301 cgtccaggta gacctcggtc ttgccgctgc cggtgacgcc gtcgagcagg aagctggcga
      361 atccgtccgc cgcgcgaatc gcatcggcgg cgctgcgctg ttcgggattc aggaccggcg
      421 cgggctgggt cggcgcggca ggaaaggggc ccctttcctg gtgcagcgcg acccgctcga
      481 ccagcccacg ttcgcgcaac ccgcgcatgg gggctcgcca tcccgcatgt tcggcatcca
      541 gccaggcttc gtcgcggaca tccgccaaca acgccgccag cgcctgcggc cggcccgcgc
      601 gcatcgccgg cagcgcggtg cagccggctt cggccaggcg ccagccatgg cgggtggtgt
      661 ccgccagcgg ttcgccacgg cgcagcaccg ccggcaacgc ggttgccagc acgtcgccga
      721 gtggggcatg caggtagccg gccagccact gcagcgacgc caacagctcg ccctgcagca
      781 acggcagcgc ctcgggcaac gccagcgcag gcttgggttc gatgccgggc tccggatcgc
      841 catggccgac gacgatgccg cacagctcgc gcccggcgaa cggcaccacg atgcgctggc
      901 cggccgccac cgtcgccgcg gccacgccgg gcggtggaag gtagtcgaag gtgcgcggca
      961 gcggcagcgg cagcgccacc cgcagcacgg aacggggcgc ctgcatcggc ccagtctaga
     1021 gcagcccccg gtggccgatg caaggccggc gtggggacac aggccggttt gcaccgcaca
     1081 actcaaccgg ccctgatgaa agcggcgtaa ctgcccgttc gcattgggaa aagtgcttta
     1141 tccacatcga ttgtggataa gtctgtgcat ggtcgcctac cgggggtgcg cgaagggcgt
     1201 ccggcaagcc ccgcacgggc tttggcgaaa aaaccgccat ccgggctgat tctttcaaaa
     1261 acaggcactt acccgtgaaa cccggctgtc acatgcggct ggaccgcgtg gaacatccgg
     1321 cggtgaagga tttgttacgc ctgtgcacaa ccgttttacc cacagcccgc gccactgctc
     1381 acggagccgc cgatgcgcag gctgtcaagc gttggtttcg cctgccggcg gcgacgtgcg
     1441 cggctaagat gcggcgtgca ttccggttcg caatgaccac cgcccctgga tcgccctcgc
     1501 cggcggccgt ggtcgcgttc ctgcgcggca tcgaccggcg tgcgcgcctg ttcgccaccg
     1561 tccaggccgg cgatccggcc cgcggcgcgc gggcgctggc ggtggtggcg cgggtgttcg
     1621 ccgccgaggc cgggcagtgg ccgctggcgc aatggccgca gcagtactgg cggctgctgc
     1681 tggccacccc gtcgttgcgg caggcggcag cgcccgatgg ccaggtggtt ctcgccggga
     1741 ttgcccgctt gccggcggac gcgcgcgcgg gtgtgctgct gcagctggtg gccgggcttg
     1801 gggacgccga cgccgcgttg gcgctgggcc tgccgctggc gacctaccag gcgcgcatcc
     1861 gcgacagcct gccgcgcgac ccgctcggcc agccggatgt cgatgtctgg cgcgcgtggc
     1921 gtgcggcggc gcagcgcgaa ctcgagctgg gcaatgcggc agtggcgcag gacgcgccag
     1981 cgccgcccac caccgggccc cgccgcgtcc gcgccgccac cgtgccggaa ctgccggcgc
     2041 cgcatggcgt gcgctggctg tggctggggg tcgccggctg cgtgctggcg ttcgccgcgg
     2101 cgttcttcat ccaccccgcc gggcgcgagg cgatccacca gtggttcgcc accatcaagc
     2161 gcgagccgct gccgccagcg gccgccgcga aggcgcgttt cgatgccggc gacatcgccc
     2221 tgcatcccga ccgcgagcgc ctggccgcgc cgcgcgaagc gtggttcgcg gaccgcctcg
     2281 cgctgctggc atggctggcg aatgccgaag ccggtcccgc cgctgatccc gccgcggccg
     2341 aggtgctgcc gttgccgggc gcggcccccg cggcaccgcc gtcgttgccc gacgccggcc
     2401 ggcaggcgac ccagctggcg cagcggatgc gcgcctggga tgcgctgccg ccgcgcgcgc
     2461 gcggcctgca gcgtggtcac tggcaggcgt ggcaggcgct ggaggccggc gaacgggtgc
     2521 ggctgcgcag ccttggcgaa cgcctgcgga tgtcgcccgc gccactgcag caggcactgc
     2581 gcgagcgctt cgacgcgcaa acctccgacg cgcgcgcggg ctggtggctg gggccgcggc
     2641 tgggccgcga ctggccgcgg gtggcggcgc tgttcgggta tgtcgaggcg gccgagcgcg
     2701 gaccgctgct ggacttcctg cgcacggccg gcgcggagga catcgacgcc tgcgcgcggc
     2761 ttgcgcagac caccccgccc gagcaacgcg cggcactgcg cgcgcaactg ctggcgcagc
     2821 cggccgcgca acgtggcgca tggctgcagg cgcgattgca gcgctagccg gccgcgccgc
     2881 tcacatcgcc accaggccga tgcccatgct ggcgacgatc gcgaagctca gcaggatcga
     2941 gtagcaggtg ccgagcacgc cgaacttcag cacggtcatc ggccagccct gcgcgtacac
     3001 ccgcttctgc atgagcagca ggtagagcgg catccacgcg accagcaggc cctcgaccca
     3061 gtcgaacagg tcgttggcca ggctgccgtc ggcggtgtac gcgcgcgaca gtgcgtgcag
     3121 cagcagcacc agcagcaggt ccaggcacag gaaggcgtgg ctgtgcaggc ccacgatcag
     3181 gtgttccatg tacagccggc gcttgaacag gtagatcagc ttcagcatca gcgcgaacac
     3241 cggcaccagc acgaacaggg tggtcggcac cgcgcccagc gccgcgttct tgaacgcgcc
     3301 cggatcctgc ttgaggcgcc tgacgttgtc gttggcgcgc gtcacctgct ggtgcagcca
     3361 gcggtcggcg aagccgggca gccacgtatc gaccggcttc ttcgcgtccc agcgctcgcc
     3421 gccgagactg atgttgaagt tgtcctgcat cggctccggc ggcggctggc ccttggcagc
     3481 cgcttcctgc aactggcgga tgcgtgcgct ggcggtgtcg cgcacggccc tctcgccggc
     3541 ttccaggccg gggatgccga cggcggccgg ggtgccagcc atcccggcct tcgccgtcgc
     3601 catttcggcg agctccgcat cacgtgcctt ttccgcctcg gccaccgtca tcgcgctgct
     3661 gatggggttg ttgctgcgct tgccatccat gcggatcgcg tccgcaccga gatccacggt
     3721 cagctgggcg acgaagaagg tgacgatggc gaggaagaag aacagccgca ccgggctgac
     3781 gtagcgcacc cggcggcctg cgaaatattc gcgggtgatg tggccgggcc gcgcgaacag
     3841 cggcccgatg gtgcggaaga tccgggtatc gaggttgaac acgctgtcca gcgcatcgcc
     3901 gaggatgctt gagaagtgcc gcaccaggcc cttcaccggc tgcccgcagg cgtagcagtg
     3961 atcgcccagc agcggcgcgc cgcaattgcc gcagggcgtg ccggcgggcg cggggtcggg
     4021 ggcggcaggc gcgttggtgg cggtcggttc ggtcatcggc attcacccgg gcgccgttaa
     4081 gatggcaggc cacgtgcctt cgcgccagcg cgacgcaccc ccggacccgc catgaacccc
     4141 agcgccgaca gcccacgctt cgccgacgaa gtgcgccaga gtgcgcgcct ggccgcgccg
     4201 ttggccgccg gccacctgtc gcacgggctg gtcgcgttcg tcgataccgt ggtcgccggc
     4261 cgccacggga ccaccacgct ggccgcggtc gcggtgggca ccgcgctgtt ctggctgccg
     4321 atgatgctgc cgatggggac gctgatggcg ctgccgccgt cggtatcgca actcgacggt
     4381 gcgcgccggc gcggggagat cggcccgctg tggcggcagt cgctgtggct ggcgttgggc
     4441 ctgggggcgc tgctgttcgc gctggtgacg gtgttgccgc tgatgctggg gccgatgggg
     4501 atcgccgcgg acatcatccc cggcgccacc gggttcctgc atgcgatccg ctggggcatc
     4561 cccgcgttca ctgtgtacct gtgcatgcgt tatctcagcg atggcctgca ctggtcgttg
     4621 ccgaccatgc tgatcggctt cggtggcttg ctgctgctgg cgccgggtgg ctacgtgctg
     4681 accaacggcc tgttcggcct gccggagatg ggtgcggcgg ggctgggctg ggcttcggcg
     4741 gcgatgttct ggggccagct gctggcgttc gcgctgtacc tgcggctgtc gccgcgtttc
     4801 gccgacttgg gcctgtacgc gcattgggaa cgcccgcagt gggcgacgat ccggggcctg
     4861 cttggccgtg ggctgccgat cggggtgacc atcaccatgg agggcagcct gttcatcgtc
     4921 accgcgctgc tgatcgggcg cctgggcgaa gtgccggtgg ccgcgcacca gatcgcgatc
     4981 aacgtcgcca gcctgtgctt catgatcccg ttcggggtgg ccgaggccac cacggtgcgg
     5041 gtcggccatg cgctgggccg cggcgaccgc gacggcatcc gccgcgcgta tttcgccggg
     5101 ctggcgctgg tgctgggcac ccaggcgctg tcggcgctgg tcatgctgct gggcaaccac
     5161 gcgatcgtcg gcctgtacac cggcgatgcg gtggtcggcg cgctggccgc atcgctgctg
     5221 ctgtatgcgg cgctgttcca gttcccggac ggcgtgcagg tgctgtcggc cggggcgctg
     5281 cgcggcctga acgacacccg ggtgccgatg tggctggcgg cgctggcgta ctggggcatc
     5341 ggcatgccgg tcggcgcggg cctcggcctg gcgctgggct gggggccacg gggcatgtgg
     5401 ctgggcctga ccgccgggct gtcggtggcg gcggtgctgc tggcccggcg cttcttgcgc
     5461 agcagcctgc gcgtccccat cgtgctgcag gcgcgaatcg aaggcgggtc atcggtcacc
     5521 gtgaccgatg ccgcatgagc tgttgacgcc gcatcggtag catgtcggca tcgtcaggac
     5581 ccgcatttgc ccgtcattcc cgcgaaaacg gggatgagga aaatccaccg gagagtttca
     5641 atgaaccaac cgcgcagccg cggccccatc gcccgggtct tcatcggcct gtgggatgtg
     5701 ctcaatttca gccgtcggct ggtgctgaac ctgctgttcc tgctggtgct gttcgtgctg
     5761 ttggcggcga tgctcggcgg cggcaagctg gcgccgctgg cggaacgctc gaccctggtg
     5821 atcgaaccgc aggggcggct ggtggagcag tacagctgcg acccggtgag ccgcgcgttc
     5881 gcgcgcgcca ccaatggcga cgactgccgg gaaatccgcc tgcgcgacct gttgcgggcg
     5941 ctggagtccg cgcgcaccga caagcggatc gagcgggtgt tgctgcgcct ggacgaactg
     6001 cagcccggcg gcttcgccag cctgcgcgag gtcgccgcgg cgatcgccag ggtcaaggcc
     6061 gccggcaagc cggtggtcgc gcatggcgac aactacagcc agggccagta cctgctggcc
     6121 gcgcacgcca gcaaggtcta cctcgacccg atgtcgcagg gcggggtgat gctggaaggg
     6181 ctggccgggt accgccagta cttccgcgaa ggcctgcagg acaagctcgg cgtggacgtg
     6241 cacctgttca aggtcggtga gtacaagtcc gcggccgagc cctacatcct ggacgcggcg
     6301 tcgccggaat cgaaggaagc cgacctgttc tggatgaacg acgtgtggca gcggatgctc
     6361 gccgacatcg gcaaggcgcg cgggctgccg ccggcgacgc tggcggcgga catcgacgcg
     6421 atggccgagc aggtcgccgc tgccaagggc gatctcgccg cgctggcgct gcagcagaag
     6481 ctggtcgacg ggctgaagac ccgcgaagaa gtcgaagagc tgctggccga tgaaggcgaa
     6541 gccgacgaca acgccgaagg cggcttccgc caggtcgcgc tggacaccta cgtgcagcac
     6601 ctcgacggcg cgctgcagcc ggccgacatg cgcccgcagg tggcggtggt ggtggccgag
     6661 ggcgcgatcg ccggtggcga actgccgccg ggcgaagtcg gcggcgaatc cacttccgcg
     6721 ctgctgcgcg cggcgcgcga cgacgaccgc gtcaaggcgc tggtgctgcg ggtgaattcg
     6781 ccgggcggcg aggtgttcgc ctccgaacag atccgccgcg aggtggccgc gctgaagcaa
     6841 gccggcaagc cggtggtggt gtcgatgggc gatgtcgccg cctccggcgg ctactggatc
     6901 agcatgaatg ccgaccgcat ctacgccgat ccctcgacca tcaccggttc gatcggcatc
     6961 ttcggcctgt tcccgaccgc cgaccgcgcg ctggccaagc tcggcgtgcg caccgatggc
     7021 gtcggcacca cccgctacgc gggggcgttc gacctcaccc gaccgctgga tccgggggtg
     7081 gccgcggtgg tgcagtcggt gatcgacaag ggctaccgcg acttcaccgg caaggtcgcg
     7141 caggcgcgcg ggcgcagcgt ggcgcagatc gacgaagtcg cgcgtggccg ggtgtggagc
     7201 ggcgagcagg cgcggcagcg cgggctggtc gacgcgcttg gcggcctcga tgcggcggtg
     7261 gccgatgccg ccgcgcgcgc caagctcggc aagccgtcga cctggcacac ccactacctc
     7321 gaggaaaccg ccaagccgtt cgagcagtgg ttcggcggcc tggtcggcag cggcgccggc
     7381 atcggcctgc tgcgcgccac cggggtcggc gaggcgctgc tgctggagca tgtcgccagc
     7441 caggcggccg cgccgttgcg cgtcgtgcag aacgcgctga agcagcgcgg caacgtgcgg
     7501 gtgaccccgc tggcgcactg cttctgcagc gcg
//
LOCUS       DAORUK010000100         7517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_11038_length_7517_cov_18.019834, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000100 DAORUK010000000
VERSION     DAORUK010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11038_length_7517_cov_18.019834"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..733)
                     /locus_tag="PK818_07530"
     CDS             complement(89..733)
                     /locus_tag="PK818_07530"
                     /inference="COORDINATES: protein motif:HMM:NF022607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HQY82617.1"
                     /translation="MGPGPRRRITLSRLLAGAMSNPHDSGNDGFQAGCKMTSRTKWLL
                     AGIGALLVAMLAYAVAGPYLAINGIRNVVAKGEYGELVRFVDFEKLRDSVTPQVQQRI
                     VGGIIGRMGRGTTSDVVSGVSSVIAEPAIDAMVSPLGIATLLRGSALAKRLGGQVAPG
                     ERPKAVDPLQNATTNFESTSLFTATVETADGKPLVFEFTRTGLAWKLTGLRLPD"
     gene            complement(700..2145)
                     /gene="sbcB"
                     /locus_tag="PK818_07535"
     CDS             complement(700..2145)
                     /gene="sbcB"
                     /locus_tag="PK818_07535"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HQY82618.1"
                     /translation="MTASFLFYDLETFGADPRRSRIAQFAAIRTNEALEEVDEPVSFF
                     VQPADDLLPSPIATLITGIAPQDALRDGVNEAAAFARIVDEMTRPQTCSAGYNSLRFD
                     DEFVRHGLYRNFHDAYEREWKGGNSRWDLLDALRLMHALRPEGICWRQREDGGGTSFK
                     LEHLAEDNALRVGDAHEALSDVRATIGIARLFRRHQPRLWDYALRLRDKRHAASLLDT
                     TGMTPLLHVSQRYPASRLCAAPVIPLARHPLFDSRVIVLDLDSEPDALLELGADAIAE
                     RLYIRQADLPEGEARVPLKEVHVNKCPALVPWEHLREADFARLRIDPDAVLRRAARLQ
                     AVGPAIAEKVRRVYARPRAATTVDVDAALYDGFIGDGDKRLLAQVRGTPPHLLANAHY
                     AFRDARLPELLFRYRARNWPASLLPAERERWNAYRRERLHADNGWSELTLAQFNQQIA
                     ELRAAHAGDDGKQALLDGLQAWARDLDAGSR"
     gene            complement(2215..3564)
                     /locus_tag="PK818_07540"
     CDS             complement(2215..3564)
                     /locus_tag="PK818_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012438675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY82619.1"
                     /translation="MPADARALTIVGGGLAGALLATLLARRGWSVDVFERRGDPRVHG
                     YAGGRSINLALAERGLHALRQAGADDAVMGQAVMMRGRFVHPLHGAPGLQRYGRDDSE
                     VIWSINRGELNIVLLDIAESHGARLHFDHALQRIDFDASLATFLHHEVAVARPFHALV
                     GADGAGSAVRVELGKRVPLHERTEWLDHGYKELEIPPAANGDFRIEANALHIWPRGHY
                     MCIALPNDERTFTVTLFMPHAGAYPSFAGVQSADDAQALFERDFPDALPLIPALRHDY
                     EANPVGMLGTLYLDRWHLDGRAVLIGDAAHAMVPFHGQGMNCAFEDCVALADHLDQHR
                     GFAEAFAAFEAQRRPDAEAIQQMALENYIEMRDKVDDAAFLLQRQLELALQERHPGRF
                     VPHYAMVSFMRIRYSVALQRSEVQREILEDATRGLESLEGVDWDAVDAAVIARLAPL"
     gene            complement(3554..4828)
                     /gene="kynU"
                     /locus_tag="PK818_07545"
     CDS             complement(3554..4828)
                     /gene="kynU"
                     /locus_tag="PK818_07545"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HQY82620.1"
                     /translation="MTEIFADAYAHACDAVDPLRHFREEFHLPLHAGGTQAYFVGNSL
                     GLQPKGARAHVEEVLDKWATEAVEGHFTGQAQWMNYHELVRDPLARVVGALPQEVVAM
                     NSLTANLHLMLVGFYRPTRERPLLLMEAGAFPSDRYALESQVRFHGLDPAEALIEVEP
                     DVDGGTFSMAAIERAIAEHGPRLAAIVWPGVQYRTGQAFDLGEITRLGHAAGAVVGFD
                     LAHAVGNLPLQLHDSGADFAVWCHYKYLNSGPGAVAGCFVHERHARTARPRFAGWWGN
                     DAAVRFRMGPEFEPTPGADGWQLSNPPILGLAPLRAALEQFDRATMAALRAKSERLTG
                     YLEQLIDAGLGDILQVVTPREVRQRGCQLSIRVIGGRERGRDLFGFLASRGVLGDWRE
                     PDVIRISPVPLYNTHADVLRFAASVREWRDAR"
     gene            complement(4825..5850)
                     /locus_tag="PK818_07550"
     CDS             complement(4825..5850)
                     /locus_tag="PK818_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY82621.1"
                     /translation="MLKIDTHAHYLPRDWPDLAAKYGDPRFPVIHHGDDGRHRIYKDG
                     KFFREIWPKTWDPQIRIDDYAGFGVQVQVLSTVPVMFSEWARPHHALELHQALNDHMA
                     QTCRDFPRHYAGIGTLPLQSPQLAIRELERCMDELGLQGVQIGSHISGPNGVHWNLDA
                     PELFDFFQAASDLGAAILVHPWDMMGTDSMPKYWLPWLVGMPAEQSRAACCLVFGGVL
                     ERLPKLRVCMAHGGGSFPYTIGRIEHGFNMRPDLVATDNFRNPREYLKRLYFDSWVAD
                     DAALRYLLETCGIDRVMLGTDYPFPLGEQVPGEGIERLALDDAQRARIYHGTALEWLG
                     LPATRFA"
     gene            complement(5861..6424)
                     /locus_tag="PK818_07555"
     CDS             complement(5861..6424)
                     /locus_tag="PK818_07555"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HQY82622.1"
                     /translation="MLPNPINLQAWIDEHRHLLKPPVGNKCIVDDHYIVMIVGGPNAR
                     TDYHFEDGPEWFYQLEGEMVLRIQEDFDGKQGAARDIRISAGEMFYLPPRVPHSPQRM
                     AGSIGLVIERKRLAHEDDALMWFCVGCNHKLYEEFFHLVDIEQDFFKVFERFYRDDAL
                     RTCSECGALNPRPTRYDMDAPSQADIT"
     gene            complement(6429..6854)
                     /locus_tag="PK818_07560"
     CDS             complement(6429..6854)
                     /locus_tag="PK818_07560"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQY82623.1"
                     /translation="MNDAVHATAAPKAVGSYPHARRVGNLLFLSGIGPRDPATNAIAG
                     NEYFADGRVRRYDIEAQARAVFANVRAVLEASGARWEDLADVTVYLTDMARDFKAYNA
                     IWAEFFPDPAGAPCRTTLGITALPTPIAIELKCIAVLGD"
     gene            complement(6854..7516)
                     /gene="can"
                     /locus_tag="PK818_07565"
     CDS             complement(6854..7516)
                     /gene="can"
                     /locus_tag="PK818_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HQY82624.1"
                     /translation="MNRLDDLLHNNRAWAERISREDPGFFERLSRQQTPKYLWIGCSD
                     SRVPANQVVDLAPGEVFVHRNIANVVVHTDLNCLSVIQFAVDVLKVEHVLVVGHYGCG
                     GVHAALTNARVGLADNWIRHVGDVSTKHAQLLLDAGDEPLQHDRLCELNALEQVANVC
                     QTTIVRDAWARGQVLSVHGWVYTLRDGRVHDLGLNVAAADQLEPQYAAALAAIRLDRE
                     DR"
BASE COUNT         1170 a         2546 c         2611 g         1190 t
ORIGIN      
        1 tcgccgcaca acatcgattc ggcgcgccgc cacgtcgccg ctggccacgt gccgcacggg
       61 gtggccaaca tgcaggaccc gggttgagtc agtccggcag gcgcaggccg gtcagtttcc
      121 aggcaaggcc ggtgcgagtg aattcgaaca ccagtggttt gccgtccgcg gtctccaccg
      181 tcgcggtaaa cagggatgtt gattcgaagt tggtcgtcgc attctgcagc ggatcgaccg
      241 ccttcggccg ctcgcccggg gcaacctgtc cgccgaggcg cttggccagg gcgctgccgc
      301 gcaacaaggt cgcgatgcca agcggtgaca ccattgcgtc gatcgccggt tccgcgatca
      361 ccgagcttac gccggacacc acgtcggacg tggtgccacg gcccatgcga ccgatgatgc
      421 cgccgacgat ccgttgctgg acctgcgggg tcacgctatc gcgcagtttc tcgaaatcca
      481 cgaagcggac aagctcgccg tattcgcctt tcgcgaccac gttgcgaatg ccgttgatcg
      541 cgaggtacgg gccggccacc gcgtaggcaa gcatcgcaac caacagcgcg ccgatcccgg
      601 caagcagcca cttcgtcctg gacgtcatct tgcaacctgc ctggaatcca tcattgccgc
      661 tatcgtgcgg attcgacatg gccccggcaa gtagccggct cagcgtgatc cggcgtcgag
      721 gtcccgggcc catgcctgca agccatccag cagggcctgc ttgccgtcat cccccgcgtg
      781 cgcagcgcgc agttccgcga tctgctggtt gaattgcgcc agcgtcagtt ccgaccagcc
      841 attgtccgcg tgcaggcgct cgcgccggta ggcattccag cgctcgcgtt ctgcaggcaa
      901 caggcttgct ggccagttgc gggcgcggta gcggaacagc agctccggca ggcgcgcgtc
      961 gcggaaggcg taatgcgcgt tcgccagcag gtgtggcggg gtgccgcgca cctgcgcgag
     1021 caggcgcttg tcgccatcgc cgatgaagcc gtcgtacagc gcggcatcga cgtcgaccgt
     1081 cgtcgccgcg cgtgggcggg cgtaaacgcg ccgcaccttc tcggcgatcg ccgggcccac
     1141 cgcctgcagg cgcgccgcgc gccgcagcac cgcatcggga tcgatccgca gccgcgcgaa
     1201 atcggcctcg cgcaggtgtt cccatggcac cagcgccggg cacttgttga cgtgcacttc
     1261 cttcagcggg acgcgcgcct cgccttccgg caggtcggcc tggcggatgt acaggcgctc
     1321 ggcgatggcg tcggcgccga gttcgagcag ggcatccggc tcgctgtcta ggtccagcac
     1381 gatcacccgc gagtcgaaca gcggatgccg cgccagcggg atcaccggtg ccgcgcacag
     1441 gcgcgacgcc ggatagcgct gcgacacgtg cagcagcggg gtcatgccgg tggtgtccag
     1501 cagcgatgcg gcgtggcgct tgtcgcgcag gcgcagcgcg taatcccaca gccgcggctg
     1561 gtggcgccgg aacaggcggg cgatgccgat ggtcgcgcgc acgtcgctca gggcctcgtg
     1621 cgcatcgccg acgcgcaggg cgttgtcctc ggccaggtgc tcgagcttga agctggtgcc
     1681 gccgccatct tcgcgctggc gccagcagat gccctccggg cgcagcgcgt gcatcagccg
     1741 cagcgcatcc agcaggtccc agcgcgagtt gccgcccttc cattcgcgtt cgtaggcgtc
     1801 gtggaaattg cggtagaggc cgtggcggac gaattcgtcg tcgaagcgca gcgagttgta
     1861 accggcactg caggtctgcg ggcgcgtcat ttcatcgacg atgcgggcga acgccgcggc
     1921 ctcgttcacg ccgtcgcgca gcgcatcctg cggcgcgatg ccggtaatca gggtggcgat
     1981 cggcgatggc agcaggtcgt cggccggctg cacgaagaac gagaccggct cgtcgacctc
     2041 ctccagcgct tcgttggtgc ggatcgcggc gaactgggcg atccggctgc ggcgcgggtc
     2101 ggcgccgaag gtctccaggt cgtagaacag gaagctggcg gtcatgtgcg tattgtagga
     2161 gtggcgtaag ccgcgaccgc tttccgggga tcctggtcgc gcccttctcc gcgcctacag
     2221 gggcgcgagg cgggcgatga ccgcggcatc cacggcatcc cagtccacgc cttccagcga
     2281 ttccagcccg cgcgtggcgt cttccagtat ttcgcgctgc acctcgctgc gctgcagggc
     2341 caccgaatag cggatgcgca tgaagctgac catcgcgtag tgcggcacga agcggcccgg
     2401 gtggcgctcc tgcagggcca gttccagctg gcgctgcaac aggaacgccg cgtcgtcgac
     2461 cttgtcgcgc atctcgatgt agttctccag cgccatctgc tggatcgcct cggcatccgg
     2521 cctgcgttgc gcctcgaagg cggcgaacgc ctcggcgaag ccccggtgct ggtccaggtg
     2581 gtcggccagg gcgacgcaat cctcgaacgc gcagttcatg ccctggccgt ggaacggcac
     2641 catcgcgtgg gcggcatcgc cgatcagcac ggcgcggccg tcgaggtgcc agcggtccag
     2701 gtacaaggta ccgagcatgc cgaccggatt ggcctcgtag tcgtgccgca atgcggggat
     2761 caacggcagc gcatccggga agtcgcgttc gaacagcgcc tgcgcatcgt cggcggactg
     2821 caccccggcg aagctcgggt atgcgccggc atgcggcatg aacagggtca cggtgaaggt
     2881 ccgctcgtcg ttcggcaggg cgatgcacat gtagtggccg cgcggccaga tatgcagggc
     2941 gttggcttcg atccggaaat cgccgttcgc ggccggcggg atctccagtt ccttgtaacc
     3001 gtggtcgagc cattcggtgc gttcgtgcag cggcacccgc ttgccgagct cgacgcgcac
     3061 ggccgagccg gcgccatccg cgccgaccag cgcgtggaag gggcgcgcca ccgccacctc
     3121 gtggtgcagg aaggtggcga gtgacgcgtc gaagtcgatc cgctgcaggg cgtggtcgaa
     3181 atgcaggcgc gcgccgtgcg attcggcgat gtcgagcagg acgatgttga gctcgccgcg
     3241 gttgatcgac cagatcacct cggaatcgtc gcgcccgtag cgctgcaggc cgggcgcgcc
     3301 atgcaggggg tgcacgaagc gtccgcgcat catcaccgcc tgtcccatca ccgcatcgtc
     3361 ggcaccggcc tggcgcaagg cgtgcaggcc gcgctcggcg agcgcgaggt tgatcgaacg
     3421 gccgccggcg tagccgtgga cgcgcggatc gccgcggcgc tcgaacacgt ccaccgacca
     3481 cccgcgccgg gcgagcaggg tggcgagcag ggcgccggcg aggccaccac cgacgatcgt
     3541 cagtgcgcgc gcgtcagcgg gcatcacgcc actcccgtac gctggcggcg aaacgcagga
     3601 cgtcggcatg ggtgttgtaa agcggcaccg gcgagatgcg gatcacgtcg ggttcgcgcc
     3661 agtcgccgag cacgccgcgc gaagcgagga atccgaacag atcgcggcca cgttcgcgtc
     3721 cgccgatcac gcggatcgac aactggcagc cgcgttgccg cacctcccgc ggagtcacga
     3781 cctgcaggat gtcgccaagg ccggcatcga tcagctgctc caggtagccg gtgagccgtt
     3841 ccgacttcgc gcgcagggcc gccatcgtcg cccggtcgaa ctgttccagc gcggcacgca
     3901 gcggcgccag gccgaggatc ggcgggttcg acagctgcca gccgtccgcg cccggggtcg
     3961 gctcgaattc gggacccatc cggaaacgca ccgccgcatc gttgccccac cagccggcga
     4021 agcgcgggcg cgcggtacgc gcgtggcgct cgtgcacgaa gcagccggca accgcgcccg
     4081 ggcccgagtt caggtacttg tagtggcacc agaccgcgaa atccgcgccg gaatcgtgca
     4141 gttgcaacgg caggttgccg accgcgtggg caaggtcgaa gccgaccacg gcgccggcgg
     4201 cgtggcccag ccgcgtgatt tccccgaggt cgaacgcctg cccggtgcgg tactgcacgc
     4261 ccggccagac gattgccgcc agccgcggcc cgtgctccgc gatcgcgcgt tcgatcgccg
     4321 ccatcgagaa ggtgccgccg tccacgtcgg gttcgacctc gatcagcgcc tcggccggat
     4381 ccaggccgtg gaagcggacc tgcgattcca gcgcatagcg gtccgacggg aaggcgccgg
     4441 cttccatcag cagcaacggg cgctcgcggg tcgggcggta gaagccgacc agcatcaggt
     4501 gcaggttggc ggtcagcgaa ttcatcgcca ccacttcctg cggcagcgcg ccgaccaccc
     4561 gcgccagcgg atcgcgcacc agttcgtgat agttcatcca ttgcgcctgg ccggtgaaat
     4621 ggccctcgac ggcttccgtc gcccacttgt ccagcacctc ctcgacgtgg gcgcgcgcgc
     4681 cctttggctg caggcccagc gaattgccga cgaaataggc ctgcgtgccg cctgcgtgca
     4741 gcggcaggtg gaactcctcg cggaaatggc gcagcggatc gaccgcgtcg caggcgtgcg
     4801 cgtacgcgtc ggcgaaaatc tcggtcatgc gaacctcgtg gcgggcaggc caagccattc
     4861 cagcgcggtg ccgtggtaga tccgggcgcg ctgcgcgtca tccagcgcaa ggcgttcgat
     4921 gccttcgccc ggcacctgtt cgccgagcgg gaaggggtag tcggtgccca gcatcacccg
     4981 gtcgatcccg caggtctcga gcaggtaacg cagggcggca tcgtcggcca cccacgaatc
     5041 gaaatacagg cgcttcaaat actcgcgggg gttgcggaag ttgtcggtgg cgaccaggtc
     5101 cgggcgcatg ttgaagccgt gctcgatgcg gccgatggtg taggggaagc tgccgccgcc
     5161 gtgggccatg cacacgcgca gtttcggcag gcgttccagc acgccgccaa aaaccaggca
     5221 gcaggccgcg cgcgactgtt ccgcgggcat gcccaccagc cacggcagcc agtacttggg
     5281 catgctgtcg gtgcccatca tgtcccacgg atgcaccagg atcgccgcgc cgaggtcgct
     5341 ggcggcctgg aagaaatcga acagttccgg cgcgtcgagg ttccagtgca ccccgttggg
     5401 gccgctgatg tgcgagccga tctgcacgcc ctgcaggccg agctcgtcca tgcagcgctc
     5461 cagctcgcgg atcgccagtt gcgggctctg cagcggcagc gtgccgatgc ccgcgtaatg
     5521 gcgcggaaag tcgcggcagg tctgggccat gtggtcgttc agggcctggt gcagttccag
     5581 cgcgtggtgc ggcctggccc attcgctgaa catcaccggc accgtgctca gcacctgcac
     5641 ctgcaccccg aagccggcgt agtcgtcgat gcggatctgc gggtcccagg tcttcggcca
     5701 gatctcgcgg aagaacttgc cgtccttgta gatgcggtgg cggccgtcgt cgccgtggtg
     5761 gatcaccggg aagcgcggat caccgtactt cgcggccagg tcgggccagt cgcgcggcag
     5821 gtagtgggcg tgggtgtcga tcttcagcat gcgcggcggc tcaggtgatg tccgcctgcg
     5881 acggcgcgtc catgtcgtaa cgcgtcggcc gcggattgag cgcgccgcat tcgctgcagg
     5941 tgcgcagcgc gtcgtcgcgg tagaagcgtt cgaacacctt gaagaaatcc tgctcgatgt
     6001 ccaccaggtg gaagaattcc tcgtacagct tgtggttgca gccgacgcag aaccacatca
     6061 gggcatcgtc ctcgtgcgcc aggcgcttgc gctcgatgac caggccgatg ctgcccgcca
     6121 tccgctgcgg cgagtgcggc acccgcggcg gcaggtagaa catctcgccg gcgctgatcc
     6181 ggatgtcgcg cgcagcgccc tgctttccat cgaaatcttc ctggatgcgc aggaccatct
     6241 cgccctcgag ctggtagaac cactccggac cgtcctcgaa gtggtaatcg gtgcgcgcgt
     6301 tcgggccgcc gacgatcatc acgatgtagt ggtcgtcgac gatgcacttg ttgccgaccg
     6361 gcggcttcag caggtgccgg tgctcgtcga tccacgcctg caggttgatc gggttcggca
     6421 gcatggcgtc agtcccccag cacggcgatg cacttgagct cgatcgcgat cggcgtcggc
     6481 agggcggtga tgccgagggt ggtgcggcag ggcgcgccgg ccggatccgg gaagaactcg
     6541 gcccagatcg cgttgtaggc cttgaagtcg cgcgccatgt cggtgaggta gacggtgacg
     6601 tcggccaggt cctcccagcg tgcgccactg gcctccagca ccgcgcgcac gttggcgaac
     6661 accgcgcgcg cctgcgcctc gatgtcgtac ctgcgcaccc gcccgtcggc gaaatactcg
     6721 ttgccggcga tggcgttggt ggcggggtcg cgcgggccga tcccggacag gaacagcagg
     6781 ttgccgaccc gccgcgcgtg cgggtagctg ccgacggcct tcggcgccgc ggtggcgtgc
     6841 acggcatcgt tcatcagcgg tcctcccggt ccagcctgat cgccgccagc gcggcggcgt
     6901 actgcggttc cagctggtcc gcggcggcca cgttgaggcc gaggtcgtgc acccgcccat
     6961 cgcgcagggt gtacacccag ccatgcaccg acaacacctg cccgcgcgcc caggcatcgc
     7021 ggacgatggt ggtctggcag acgttggcga cctgctcaag tgcattgagc tcgcacaggc
     7081 gatcgtgctg cagcggttcg tcgccggcat ccagcagcaa ctgcgcatgc ttggtggaga
     7141 cgtcgccgac atggcggatc cagttgtcgg ccaggcccac ccgcgcattg gtcagcgccg
     7201 catgcacgcc gccgcagcca tagtggccga ccacaaggac atgctcgacc ttgagcacgt
     7261 cgaccgcgaa ctggatcacc gacaggcagt tgaggtcggt gtgcaccacg acattggcga
     7321 tgttgcgatg cacgaacacc tcgcccgggg cgaggtccac cacctggttc gccggcactc
     7381 gcgaatcgga acagccgatc cacaggtatt tcggcgtctg ctgccgcgac aaccgttcga
     7441 agaaacccgg gtcctcgcgc gagatgcgtt cggcccaggc gcggttgttg tgcagcaggt
     7501 cgtcgagtcg gttcatg
//
LOCUS       DAORUK010000101         7494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_11099_length_7494_cov_17.074338, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000101 DAORUK010000000
VERSION     DAORUK010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11099_length_7494_cov_17.074338"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="PK818_07570"
     CDS             complement(<1..1339)
                     /locus_tag="PK818_07570"
                     /inference="COORDINATES: protein motif:HMM:NF014312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HQY82625.1"
                     /translation="MQRKHALTALALALFGGASAYAVGQITNERPPQGTLEAGEATFL
                     ANCAACHQPTGKGLPGAFPPLADSDYMKALPKDKLIGVVLNGFSGKVTVNGMDYDGVM
                     PALNHLSDTEIANTLTYVLNAWGNNSGSVSATEVAAARGEQTQQAAKSGTATPEAKYQ
                     GAPSTVTVADASAATGLPPLTDAERKESTQIFFERCAGCHGVLRKGATGKPLTPDITR
                     VKGTDYLKALINFGSPAGMPNWGTSGQLTAAQIDTMARFLQHEPPAPPEWGMKEMKDS
                     MRVFVPVEKRPTKKMNKYNTDNIFAVTLRDSGEVALVDGDTKKIINIVTTGYAVHISR
                     PSHSGRYLYTIGRDARIDLIDLWLPKPDKVAEIKIGLEARSVETSKYKGYEDKYAIAG
                     AYWPPQYVIMDGETLNPLKIVATRGMTVDTQEYHPEPRVAAIVASHEHPEFIVN"
     gene            1574..3784
                     /locus_tag="PK818_07575"
     CDS             1574..3784
                     /locus_tag="PK818_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HQY82626.1"
                     /translation="MPRKRPLPRILACLLWMPAAAQAGEADPEQARRTLDRVVVVASK
                     VAEPVRQLVGTVSEIDRATMERRQVQTIADLVRYEPGVDAIGDGHRFGWQGFAIRGLD
                     GNRVGMEIDGVPVADAFSVGEFAAAGRDLLDLDAVQRVEILRGPASTLYGSDALAGIV
                     AMRTRDPQDLLGRVGADRYAGGRIGWSGRDHGRLLSAAFAAASGPLQGLLQATRREAH
                     ETENASSTLPANPAELARTSLLAKLVFEGPGADRWTATLDHGSGEVNTDVKSLRFGPG
                     RFATTTSLLGDDSYQRDRASVQYEWEPGQAWLEQAELLLYAQRNRTRQDSAQERLADR
                     TTRFRSLRERRFELRQDDVGLEFLGQAHQQGERVRHWHVFGFEMAGHRYLGQRDGRET
                     NLDTGTSSNVILGERFPVRDFPPSRSRELGLFWQDEIGFGEAFALIPGLRWERYQLQA
                     EADAMFREDYPLQEVVDVAHSAWTPKLGARWHASDRSTWFAQYARGFRAPPFGDVNIG
                     LSLALLNYEVRPNPELQPETSAGLEAGWRWQDERLQASVSIYRNQYRDLIESRANLGI
                     DPDTGALVFQSVNRARADIHGVEASMQWVPDAAGDWRLSGSAAWARGRDTARDRPLNS
                     VAPARLVLGAAWEPVSDRFGAELVATAVARQGELDTSAGPLFRAPGYALLDLHAWLRI
                     TTGVRLRFSVENLGDRTYWNWSSTRGINLAQASPPLDFHTRPGRSISLGLDVDW"
     gene            complement(3846..5369)
                     /locus_tag="PK818_07580"
     CDS             complement(3846..5369)
                     /locus_tag="PK818_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQY82627.1"
                     /translation="MLFERRKPLDLILETASKRSLTRQLGAFDLTMLGIGAVIGTGIF
                     VLTAVAANKAGPGMMYSFVIAGIVCALTALIYSEIAAMVPVSGSAYTYSYAVLGEIIA
                     WLVGWALILEYAVAAGAVAVGWSGYANGFLREIGAGLPIALTAGPGDTITLPDGTTAA
                     GSFNLIAFLLSLLITWLLYLGTSKSAKFTAMLVLVKIIALAVFIFLAFPAVNSANFEP
                     MLPNGWGTPLSGVGVLGAAASIFFAYVGFDAVSTAAEETRNPNRNIPIGLIGSLAVCT
                     IFYLLVAYAAVGAVGAQPGGALSQSKEPLAFVLREIGWPKVGSAVAIAAIVALPSVVL
                     MMIFGQTRILFTMARDGLLPDVLAKVHPKYHTPHVVTWVTGIAVSLFAAMFPVGMLAD
                     ISNSGTLFAFFMVAAGVMVLRVTDPKRIRPFRAPLIWIVGPLAMAGCVLLFFSLGWNP
                     TIKYFCIWAVIGLVLYFLYARRRSHLAPGNEYLLHGYAKDGKLEPDPLVQEGPNAGP"
     gene            5495..6058
                     /locus_tag="PK818_07585"
     CDS             5495..6058
                     /locus_tag="PK818_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQY82628.1"
                     /translation="MDRSGSDDLADAFDAYARRWPAEAATAALFGELLAQPGDPFRRE
                     RLAGHFTASCWLVDAGGTRALLTHHRKLGLWLQPGGHADGERDLRVAALKEAREESGL
                     AGLRMEDGIFDLDRHWIPEHKRVPAHWHYDVRYVVRAGTDEAYVVSDESHDLAWRAIA
                     DLAIDAGADDSVRRMAGKWLARGAGQG"
     gene            complement(6194..6760)
                     /gene="yeiP"
                     /locus_tag="PK818_07590"
     CDS             complement(6194..6760)
                     /gene="yeiP"
                     /locus_tag="PK818_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009603592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HQY82629.1"
                     /translation="MKAYEIKKGNVVEHNGSVYQVRDIERSSPQGRGGNVKFRFTMYS
                     VPGGTKFDLSLGGDDELKEVELSRRQVTYSYRDGDAFVFLDDEDYTPYTLDADVVGDA
                     AGYIVDDLTGCHVQIIDDLPVAVQLPASVAIEVIETPPELKGGTATKRPKPARLSTGI
                     EIMVPEYIGNGEKVWVNTATGEFAGRAD"
     gene            complement(6762..7055)
                     /locus_tag="PK818_07595"
     CDS             complement(6762..7055)
                     /locus_tag="PK818_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82630.1"
                     /translation="MTDYGSPAERWVATRWPAIRARGMWRFLLVKGVALWGGLMFVFM
                     AVVMALQLGAAHPRLPLVLAVALPLCAIGGLAWGVLTWIFNERIFRALPTMKR"
     gene            complement(7052..>7494)
                     /locus_tag="PK818_07600"
     CDS             complement(7052..>7494)
                     /locus_tag="PK818_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY82631.1"
                     /translation="TVMVNLVGSFNVAKACAEQMQRNEAGTDGERGVIVNTASVAAYE
                     GQIGQAAYSASKAGVVGMTLPMARELARFGIRVMTVAPGAFWTPMVDGMPDEVQQSLA
                     ASIPFPSRLGQAPEFADLVAYILTNTYLNGETIRLDGATRLAPK"
BASE COUNT         1168 a         2538 c         2600 g         1188 t
ORIGIN      
        1 cgttgacgat gaactcgggg tgctcgtgcg aggccacgat cgcggccacg cgcggttccg
       61 ggtggtattc ctgggtgtcg acggtcatcc cgcgggtggc gacgatcttc agcgggttga
      121 gggtctcgcc gtccatgatc acgtactgcg gcggccagta ggcaccggcg atcgcgtact
      181 tgtcctcgta gcccttgtac ttggaggtct cgaccgaacg cgcctcaagc ccgatcttga
      241 tttccgcgac cttgtccggc ttcggcagcc acaggtcgat caggtcgatg cgcgcgtcgc
      301 ggccgatggt gtacaggtag cggccggaat gcgaaggccg cgagatgtgc accgcgtagc
      361 cggtggtgac gatgttgatg atcttcttgg tgtcgccgtc caccagcgcc acttcgccgc
      421 tgtcacgcag ggtgaccgcg aagatgttgt cggtgttgta cttgttcatc ttcttggtcg
      481 gccgcttctc caccggcacg aacacccgca tgctgtcctt catctccttc atgccccatt
      541 ccggcggtgc cggcggttcg tgctggagga agcgcgccat ggtgtcgatc tgggcggcgg
      601 tcagctggcc ggaggtgccc cagttcggca tgccggcggg ggagccgaag ttgatcaggg
      661 ccttgaggta gtcggtgccc ttgacccggg tgatgtccgg ggtcagcggc ttgccggtgg
      721 cgcccttgcg cagcacgccg tggcagccgg cgcagcgttc gaagaagatc tgggtggatt
      781 ccttgcgctc ggcgtcggtc agcggcggca ggccggtggc ggcgctggcg tcggccacgg
      841 tcaccgtgct cggcgcgccc tggtacttcg cttccggggt cgcggttccg gacttggccg
      901 cctgctgggt ctgctcgccg cgcgcggcgg cgacctcggt ggcgctgaca ctgccgctgt
      961 tgttgcccca cgcgttcagc acgtaggtca gggtgttggc gatctcggtg tcgctgaggt
     1021 ggttcagcgc cggcatcacg ccgtcgtagt ccatgccgtt gaccgtgacc ttgccggaaa
     1081 agccgttcag gaccaccccg atcagcttgt ccttcggcag cgccttcatg tagtcggagt
     1141 ctgcaagcgg cgggaacgcg ccgggcaggc ccttgccggt cggctggtgg cacgccgcgc
     1201 agttggccag gaaggtggct tcgcccgcct ccagggtgcc ctgcggcggg cgctcgttgg
     1261 tgatctggcc gacggcgtag gcggaggcac cgccgaacag tgcgagggct agggcagtga
     1321 gtgcgtgttt gcgttgcatt tgactctccc cgtgacgaaa tgaagcgcgt ggccatcagg
     1381 ccttgccggc attgtccggc cagcttccgg gcgaatggtt gacatgggtc aagtgccgac
     1441 gtgtcggcca gttgacccag ctcaagccgg gcgggtttgc gcacgcggcg gcccggatcg
     1501 cggcgggtga acgcctacac ttgcgaaccg tccccacgac ccgcgaccac cgccgatgcc
     1561 cggatcctgc cctttgcccc gcaagcgacc gcttccgcgc atcctggcct gcctgctgtg
     1621 gatgccggcg gcagcgcagg cgggggaggc cgacccggag caggcgcggc gcaccctgga
     1681 ccgggtggtg gtggtggcca gcaaggtcgc cgagccggtc cgccagctgg tggggacggt
     1741 cagcgagatc gaccgcgcga ccatggagcg ccgccaggtg cagacgatcg ccgacctggt
     1801 gcgctacgaa cccggcgtgg atgcgattgg cgatggccac cgcttcggct ggcagggctt
     1861 cgcgatccgc gggctggacg gcaaccgggt tggcatggaa atcgatgggg tgccggtcgc
     1921 tgacgcgttc agcgtgggcg agttcgccgc cgccgggcgc gacctgctcg acctcgacgc
     1981 ggtgcagcgg gtcgagatcc tgcgtggccc ggcctccacc ctgtacggca gcgatgccct
     2041 ggcaggcatc gttgcgatgc gcacccgcga cccgcaggac ctgctcggcc gggtcggcgc
     2101 cgatcgctat gccggcggtc gcatcggctg gtccgggcgc gatcacggcc gcctgctgtc
     2161 ggccgcattc gccgccgcgt ccggcccgct gcagggcctg ttgcaggcga cccgccgcga
     2221 ggcccacgaa accgaaaacg cctcgtccac gctgccggcg aaccccgcgg aactggcgcg
     2281 gacctcgctg ctggccaagc tggtcttcga gggaccgggc gccgaccgct ggaccgccac
     2341 cctcgaccat ggcagtggcg aggtgaacac cgacgtcaag tccctgcgtt tcgggccggg
     2401 ccgcttcgcc accaccacct cgctgctggg cgacgattcc taccagcgcg accgcgccag
     2461 cgtgcagtac gaatgggagc ccggccaggc ctggctggag caggccgaac tgctgctcta
     2521 cgcgcagcgc aaccgcaccc gccaggactc cgcgcaggag cggctggcag atcgcacgac
     2581 ccgcttccgc tcgctgcgcg agcgccgctt cgagttgcgc caggacgatg tcggcctgga
     2641 gttcctcggc caggcccacc agcagggcga acgcgtgcgg cactggcacg tgttcggatt
     2701 cgaaatggcg ggccaccgtt acctcggcca gcgcgatggg cgcgagacca acctcgacac
     2761 cggtaccagc agcaacgtga tccttggcga gcgcttcccg gtgcgcgatt tcccgcccag
     2821 ccgcagccgc gagctgggcc ttttctggca ggacgagatc ggcttcggcg aggcgttcgc
     2881 gctgatcccc ggcctgcgct gggagcgtta ccagttgcag gcggaggcgg acgcgatgtt
     2941 ccgcgaggac tatccgctgc aggaggtggt ggacgtcgcg cattcggcat ggacgccgaa
     3001 actgggcgcg cgctggcacg ccagcgatcg ctcgacctgg ttcgcgcagt acgcccgtgg
     3061 cttccgcgca ccaccgttcg gcgacgtcaa catcggcctg tcgctggccc tgctcaatta
     3121 cgaagtgcgg ccgaatcccg aactgcagcc ggaaaccagt gccgggctcg aggccggttg
     3181 gcgctggcag gacgagcgcc tgcaggccag cgtgtcgatc taccgcaacc aatatcgcga
     3241 cctgatcgag tcgcgcgcga acctcggcat cgacccggat accggcgcac tggtgttcca
     3301 gtcggtcaac cgcgcccgcg ccgacatcca cggcgtcgaa gccagcatgc agtgggtgcc
     3361 ggatgccgcc ggcgactggc gcttgagcgg atcggcggcg tgggcgcgcg gccgcgacac
     3421 cgcgcgcgac cgtccgctca acagcgtcgc cccggccagg ctggtgctgg gcgcggcctg
     3481 ggagccggtt tcggatcgct tcggcgcgga actggtggcc actgcggtgg cgcgccaggg
     3541 cgaactcgat acatcagccg gaccgctgtt ccgcgcaccg ggatatgcgc tgctggacct
     3601 gcacgcatgg ttgcggatca cgaccggcgt gcgcctgcgg ttttccgtgg aaaacctggg
     3661 cgaccgcacc tactggaact ggagcagcac gcgcggcatc aacctggcgc aggccagccc
     3721 gccgctggat ttccacacgc gccccggccg cagcatttcg ctgggtctcg acgtcgactg
     3781 gtaggcgggc ctcgcacaac gaaaaacccg gccgaagccg ggtttttcgt cgaagcggct
     3841 ggcgcttacg ggccggcgtt cgggccttcc tggaccagcg ggtccggttc cagcttcccg
     3901 tccttggcgt agccgtgcag caggtactcg ttgcccggcg ccaggtggct gcggcgacgt
     3961 gcgtacagga agtacagcac cagcccgatc accgcccaga tgcagaagta cttgatggtc
     4021 gggttccagc cgaggctgaa gaacagcagc acgcaaccgg ccatcgccag cgggccgacg
     4081 atccagatca gcggcgcgcg gaacggacgg atccgcttcg ggtcggtcac ccgcagcacc
     4141 atcacgccgg ccgcgaccat gaagaacgcg aacagggtgc cggagttgga gatgtcggcc
     4201 agcatgccca ccgggaacat cgccgcgaac agcgagaccg cgatgccggt cacccaggtc
     4261 accacgtgcg gggtgtggta tttcgggtgg accttggcca gcacgtcagg cagcaggccg
     4321 tcgcgggcca tcgtgaacag gatgcgggtc tggccgaaga tcatcatcag caccaccgag
     4381 ggcagggcga cgatggcggc gatcgccacc gcgctgccga ccttcggcca gccgatctcg
     4441 cgcagcacga acgccagcgg ctccttcgac tgcgacagtg cgccgcccgg ctgcgcgccc
     4501 accgcgccga ccgcggcata ggcgaccagc aggtagaaga tcgtgcacac cgccagcgaa
     4561 ccgatcaggc cgatcgggat gttgcggttc gggttcctgg tttcttccgc agcggtggaa
     4621 actgcgtcga agccgacata ggcgaagaag atcgatgccg cggcacccag cacgcccacg
     4681 cccgacagcg gcgtgcccca gccgttcggc agcatcggct cgaagttggc cgaattcacc
     4741 gcagggaacg cgaggaagat gaagaccgca agggcgatga tcttcaccag caccagcatc
     4801 gcggtgaact tcgccgactt ggaggtgccg aggtagagca accaggtgat cagcagcgac
     4861 agcaggaacg cgatcaggtt gaagctgccg gcggcggtgg tgccgtcggg cagggtgatg
     4921 gtgtcgcccg gccctgcagt gagggcgatc ggcaatcccg cgccgatttc ccgcaggaat
     4981 ccgttggcgt agccggacca tcccaccgcc accgcgcctg ccgcgacggc atattccagg
     5041 atcagggccc agcccaccag ccacgcgatg atttcgccga gcacggcgta ggagtaggtg
     5101 taggcggaac ccgacaccgg caccatcgcc gcgatttcgg aatagatcag cgccgtcagc
     5161 gcgcagacga tgccggcgat gacgaacgag tacatcatcc cgggcccggc cttgttggcc
     5221 gccaccgcgg tcaggacgaa gatgccggtg ccgatcacgg cgccgatccc gagcatggtc
     5281 aggtcgaagg cgccaagctg gcgggtcagc gagcgcttgc tcgccgtctc aaggatcagg
     5341 tcaagcggtt tcctgcgttc gaacaacatc aatatcccct cgggccatgc ggcattggaa
     5401 tcacgcgacc ttaactgccg tcgccgcatc gcacaagctg ccatgcacgc caaacggcga
     5461 taatccgcgt cccaattcga tgatgcatcg caacatggat cgatccggtt ccgacgacct
     5521 cgccgacgcc ttcgacgcat acgcccggcg ctggcctgcg gaggcggcga ccgccgcgct
     5581 gttcggtgaa ttgctggcgc agcccgggga cccgttccgg cgcgaacgcc tggccgggca
     5641 cttcactgct tcgtgctggc tggtggatgc cggcggcacc cgcgccttgc tgacccacca
     5701 ccgcaaactc gggctgtggc tgcagccggg tgggcacgcc gacggcgagc gcgacctgcg
     5761 cgtcgccgca ttgaaggaag cacgcgaaga gtcgggcctg gccggcctgc gcatggagga
     5821 cggcatcttc gacctcgacc gccactggat cccggaacac aagcgcgtgc cggcgcattg
     5881 gcattacgac gtgcgctacg tggtgcgcgc gggcacggac gaggcctacg tggtcagcga
     5941 cgaatcgcac gacctggcgt ggcgcgcgat cgccgacctg gcgattgatg cgggcgccga
     6001 cgactcggtg cggcggatgg ccggcaagtg gctggcgcgg ggtgccggac aggggtgacg
     6061 cagcgacgcg cggcgaagca aggaccggcg ggaattcgct gcgcggctcg aaatcccgct
     6121 gcgcgaacac ccgccaatcc cggcttcgcc gtgcaaccgt ccagcgtgac caccacgcca
     6181 gcggcgggtt cgatcagtcc gcgcgtcccg cgaattcgcc ggttgcggtg ttgacccaca
     6241 ccttctcgcc gttgccgatg tactccggca ccatgatctc gatgccggtg ctgagcctgg
     6301 ccggcttcgg gcgcttggtg gcggtcccgc ccttgagctc cggcggggtt tcgatgactt
     6361 cgatcgccac gctcgcgggc aactgcaccg ccaccggcag gtcgtcgatg atctggacat
     6421 ggcagccggt gaggtcgtcg acgatgtaac cggcggcatc cccgaccacg tccgcgtcca
     6481 gcgtgtacgg cgtgtagtcc tcgtcgtcga ggaacacgaa ggcatcgccg tcgcggtagg
     6541 agtaggtgac ctgccggcgc gagagctcga cttccttcag ctcgtcgtcg ccgccgaggc
     6601 tgaggtcgaa cttggtgccg ccgggcacgc tgtacatggt gaagcggaac ttgacgttgc
     6661 cgccgcggcc ctgcggtgaa ctgcgttcga tgtcgcggac ctggtacacg ctgccgttgt
     6721 gttcgaccac gttgcccttc ttgatctcgt aagccttcat ctcatctctt catggtcggg
     6781 agcgcgcgga aaatgcgctc gttgaaaatc caggtgagca cgccccaggc gaggccgccg
     6841 atcgcgcaca gcggcagcgc gaccgccagc accagcggca gccgcgggtg ggcggcgccg
     6901 agttgcagcg ccatcaccac tgccatgaac acgaacatca gtccgcccca cagggccacg
     6961 cccttcacca gcaggaagcg ccacatcccg cgtgcgcgga tcgccggcca gcgggtggcg
     7021 acccagcgct cggcaggcga gccgtaatcg gtcacttcgg cgccaggcgg gtggcgccat
     7081 cgaggcggat ggtctcgccg ttgaggtagg tgttggtgag gatgtaggcc accaggtcgg
     7141 cgaactccgg cgcctggccc agccgcgacg ggaacgggat cgacgccgcc agcgactgct
     7201 gcacctcgtc cggcatgcca tccaccatcg gcgtccagaa cgcgccaggc gccaccgtca
     7261 tcacccggat gccgaagcgg gccagctcgc gcgccatcgg cagggtcatg ccgaccacgc
     7321 cggccttcga cgccgaatac gcggcctggc cgatctggcc ctcgtacgcc gccaccgagg
     7381 cggtgttgac gatcaccccg cgctcgccgt cggtgccggc ctcgttgcgc tgcatctgct
     7441 cggcgcaggc cttggcgacg ttgaagctgc cgaccaggtt gaccatcacc gtgg
//
LOCUS       DAORUK010000102         7422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_11242_length_7422_cov_19.401385, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000102 DAORUK010000000
VERSION     DAORUK010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7422
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11242_length_7422_cov_19.401385"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1798
                     /locus_tag="PK818_07605"
     CDS             2..1798
                     /locus_tag="PK818_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HQY82632.1"
                     /translation="MHGGGLELALVFLLAAVVAVPVFKRFGLGAVLGYLVAGVLLGPH
                     ALRVIADAEPVLAASEIGVVMLLFVIGLELSLPRLTVMRRPIFGVGGLQVLLCGGVLA
                     ALALGSGMGWKTALVVGAGLALSSTAVGLQLLSERKELGSEHGRLAFAILLFQDLAAI
                     PLLAAIPLLGKAGAADLGMADVASALLAITALVLGGRYLLRHLFRIIARTGMPEVFTA
                     TALLVVLGSAWLLQLAGLSAGLGAFLAGVLLAESEYRHELEAQIRPFEGLLLGLFFMA
                     VGMSIDLQRIAAEPLLIAAGVALLLAVKFALLFALGLRPGRLDVRGALLLGGVLALGG
                     EFAFVVFGEAAKAGLLDAAARDRLVAIVGLSMAATPLLLMLITRLAPEPHPHAAPAYD
                     RIPDEHPQVLVAGFGRFGQIVARLLWSQKIRFVAIDPDVAQVDLSRRFGNPIYYGDPT
                     QPELLRAAGAAGVKVFVVAIDGVEASLRMVRLLRTRYPDGRVFARARDRRHAWQLMDL
                     GAHVFREFFGSSVEMGREVLVALGMSPEQAADRARRFRAFDEKLLEAQRQLQDDEDAL
                     LQQAQDAGRELSELFEADRGEGSLGRIVERGD"
     gene            complement(1847..5107)
                     /locus_tag="PK818_07610"
     CDS             complement(1847..5107)
                     /locus_tag="PK818_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQY82633.1"
                     /translation="MLLAAACWFDVGTAGALAWLAQVPAALGIALLRGTRGSARGGRD
                     ALALALLWAAGFIFAVLLAAWPLQALRASPGLLPTLVMSAASAVALLLLWRHWPLWHG
                     LEREGGGIRARFAAQAAHERGAWSGLQVAVPALLLLGGGIALAWPGLLAGFARTVATG
                     AYAALLPLAHALLQSAPSRLLVHGLPVVEMEEAEAEAEAATPAHEPPPPVAAGAVVDG
                     EARTRELYAVARAGRVERALQLLEAGADAQAPPPAGDRDRRGLPVLAAVLPDLRLLRA
                     LIARGVDLNAAQAATTPLLAATRDSWHGRPEAVMTLLTNGADPRATDADGNTPLHHAA
                     RSSDPGVAALLRDAGADVDALNGEGVSPLGIACSAGNWRLARFLLERGARPEPTGGQP
                     VLLAAAGTEEDDAAGVQLLLRHKARVDARDGRGRSALHEAAFAGHSGIVAALLDAGAD
                     VHARDGEGRTPLLESARNAALPALETLLEAHADPRDCDGAGATALHLAVAADAPSIAL
                     VKRLLALGVDAQLQDAEGRSAVDVAAAGGRWALVAALDPQRKLPIAMDDDDDGVPADR
                     PPQLLLRERLLEGAGGSELAALAQLLGSAELNGLVCDAEVAAAPARVEWLLRHGAQAE
                     ARVPGAPSPVAAALARGADGIDTLRVLFAAGGSPAGGGGFATWLGACLGQAPVPNAQR
                     FALELLERGADPYGASPAGDPPLALAVRLHWTRLLQRLIAHGVDLDARDSHGMSALHV
                     AAALGRGDALKPLVAAGAAPDRRAADGQTPLGVALAAGRRDLADWLDWRGWRLPRRPL
                     QASDVPAAAIVGDTDAVRRLLDLGLPVDAVDSQGCSALLRAAGGGHRALVDLLLARGA
                     NPALAAHTGATPLSAAVSMRQVEIVDCLLDAGAGLEQRLPGDVTVLMLAAALGLPELV
                     SRLLQAGADLQATDAQGLSPLHCATLYGFTARERPRLVALLDTLLLAGADPQQAAAGG
                     LTPLLLLLGARAEAGTPCAEEVVLAGLERLFDEEVRLDARDTRGFGPLHLAALHGLLR
                     VAQALLREGADPDQRDALNRTPREVAVMRGYVDVAAELAPPSGASSQVSMARFLRE"
     gene            complement(5148..5417)
                     /locus_tag="PK818_07615"
     CDS             complement(5148..5417)
                     /locus_tag="PK818_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HQY82634.1"
                     /translation="MLAFVYKSLKKADTYVYLAARDDFARLPEPLRTQLGALQFVLDV
                     DLAPGRTLARENPDVVRANLAGRGFHLQFPPTMLDPMTEDWGTDA"
     gene            5550..>7422
                     /locus_tag="PK818_07620"
     CDS             5550..>7422
                     /locus_tag="PK818_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HQY82635.1"
                     /translation="MRLAPLAAALLLTSLSATAAEPLRLAEAMADPDWIGPPVEDAWW
                     RWDGKAAQYILKRKGTRIGDTWQVGIDGGAPAMLEGSARADLDAASALLDTSGSRSAF
                     VRNGDVFVRDLRSGALTQLTRSNEAESRLLWSGDGALAWRGADAWYRWDGRATVQAAQ
                     AKAEDAPGTPPKPDDLRDRQLRLIETLKNDRALREAAREQGEAWRKADPTRAAAPVYL
                     GAGVDIVDSALSPDGRWLLVATTEKGAATGTAGKMPMYVTESGYEEFEDVRTRVGRAD
                     PLPQKLWLAEVATGKVSELKFDALPGIASDPLAALRKAAGKEALKGNRAVQVATSGDN
                     GDAPALLWSNDSRNAAVMVRAIDNKDRWIATVDLAAAKLQPRHRLTDPAWINWGFNDF
                     GWLADGSTLWYLSEESGYSHLYTLDGGSRAQLTDGKWEASSVVPARDGSGFWFLCNRS
                     RPGDYEVCNVPARGGAVREVTALDGVESFRLSPDGARLLVRNSSAYMPPQAYVVDAAG
                     GQAKQLTDTRTPEFKAREWLQPQIVQVPSKHGAGTIWGKYYGPATMEPGRKYPVVMFV
                     HGAGYLQNVSARYPNYFREQMFHNLLVQQGYIVLDLDYRASEGYGRDWRTAIYRWMGK
                     "
BASE COUNT         1020 a         2673 c         2755 g          974 t
ORIGIN      
        1 catgcacggc ggcggtctcg aacttgcgtt ggtgttcctg ctcgcggcgg tggtcgcggt
       61 gccggtgttc aagcgcttcg ggctgggcgc ggtgcttggc tacctggtgg ccggggtgct
      121 gctgggacca catgcgctgc gggtgatcgc cgatgcggaa ccggtgctgg cggccagcga
      181 gatcggcgtg gtgatgctgc tgttcgtgat cggcctggaa ctctcgctgc cgcggctgac
      241 ggtgatgcgc aggccgatct tcggcgtagg cgggctgcag gtgctgctgt gcggcggggt
      301 gctggcggcg ctggcgctgg gcagcgggat gggctggaag accgcgctgg tggtcggtgc
      361 cgggctggcg ctgtcgtcga ccgcggtcgg cctgcagctg ctgtccgagc gcaaggagct
      421 cggcagcgaa cacgggcggc tggcgttcgc gatcctgctg ttccaggacc tcgcggcgat
      481 cccgctgctg gcggcgatcc cgctgctcgg caaggccggc gctgccgacc tgggcatggc
      541 cgacgtggcc agtgcgctgc tggcgatcac cgcgctggtg ctgggcgggc gctacctgtt
      601 gcgccacctg ttccgcatca tcgcgcgcac cggcatgccg gaagtgttca ccgccaccgc
      661 gctgctggtg gtgctgggca gcgcctggct gctgcaactg gcgggactga gcgccggcct
      721 tggcgcgttc ctggccggcg tgctgctggc cgaatccgaa taccggcacg aactggaagc
      781 gcagatccgc ccgttcgagg gcctgctgct cgggctgttc ttcatggcgg ttggcatgag
      841 catcgacctg cagcggatcg ccgccgagcc gctgctgatc gcggccgggg tggcactgct
      901 gctggcggtg aaattcgcgc tgctgttcgc gcttggcctg cgtcccggca ggctggacgt
      961 gcgcggcgcg ctgctgctgg gcggggtgct ggcgctgggc ggcgagttcg cgttcgtggt
     1021 gttcggcgaa gccgcgaagg ccgggctgct ggacgccgcc gcgcgtgacc ggctggtggc
     1081 gatcgtcggc ctgtcgatgg cggccacccc gctgctgctg atgctgatca cgcgcctggc
     1141 gccggaaccg cacccgcacg cggcgcccgc ctacgaccgg atcccggacg aacatccgca
     1201 ggtgctggtg gccggcttcg gccgcttcgg ccagatcgtg gcgcgcctgc tgtggtcgca
     1261 gaagatccgg ttcgtggcga tcgatccgga tgtcgcgcag gtggacctgt cgcgccgctt
     1321 cggcaacccg atctattacg gcgacccgac ccagccggaa ctgctgcgcg cggccggcgc
     1381 ggccggggtg aaggtgttcg tggtggcgat cgacggcgtc gaggcgagcc tgcggatggt
     1441 gcggctgctg cgcacccgct atcccgacgg acgcgtgttc gcgcgcgcgc gcgaccgccg
     1501 gcacgcgtgg cagctgatgg acctgggtgc ccacgtgttc cgcgagttct tcggcagcag
     1561 cgtggaaatg ggacgcgagg tgctggtggc gctggggatg tcgccggagc aggcggcgga
     1621 ccgcgcacgc cgcttccgcg ccttcgacga gaagctgctg gaagcgcagc gccagctgca
     1681 ggacgacgag gacgcgctgc tgcagcaggc gcaggatgcc ggtcgcgagc tgtcggagct
     1741 gttcgaggcg gaccgcggcg agggctcgct gggacgcatc gtggaacgcg gcgactgagg
     1801 cgtcgccgtc atcccgcttc cgcgggaatg gcgatcacga acgccgttac tcccgcagga
     1861 acctcgccat cgacacctgc gagcttgcgc ctgacggcgg cgcgagttcg gcggcgacat
     1921 ccacgtagcc gcgcatcacc gcgacctcgc gcggggtgcg gttgagggca tcgcgctggt
     1981 cggggtcggc gccttcgcgc agcagcgcct gggcgacgcg cagcaggccg tgcagggcgg
     2041 cgagatggag cgggccgaag ccgcgggtat cacgcgcatc cagccgcacc tcctcgtcga
     2101 acaggcgctc aagcccggcc agcaccacct cttccgcgca cggcgtgccg gcttcggcgc
     2161 gcgcgcccag cagcagcagc agcggggtga ggccgccggc cgcggcctgc tgcgggtcgg
     2221 cgccggccag cagcagggtg tcgagcaggg cgaccaggcg cgggcgttcg cgcgcggtga
     2281 agccgtacag ggtggcgcag tgcagcggac tcagtccctg cgcgtcggtg gcctgcaggt
     2341 cggcgccggc ctgcagcagc cgcgacacca gttccggcaa ccccagcgcc gccgccagca
     2401 tcagcacggt gacatcgccc ggcaggcgtt gctccaggcc ggcgccggca tccagcaggc
     2461 agtcgacgat ctcgacctgg cgcatgctca ccgccgccga caacggggtg gcgccggtat
     2521 gcgcggccag tgccgggttc gcgccgcgcg ccagcaacag gtcgaccagc gcgcggtggc
     2581 cgccgccggc cgcgcgcagc agtgcgctgc agccctggct gtcgacggca tccaccggca
     2641 ggccgaggtc gagcaggcgg cgcaccgcat cggtatcgcc aacgatcgcc gcggccggca
     2701 cgtcgctggc ctgcagcggc cgccgcggca gccgccagcc gcgccagtcc agccagtcgg
     2761 caaggtcgcg gcggccggcg gcaagggcaa cccccagcgg ggtctggcca tcggcggcac
     2821 gccggtccgg cgcggcaccg gcggcgacca gcggcttgag ggcgtccccg cgaccaagcg
     2881 cggcggccac gtgcagtgcg ctcatgccgt ggctgtcgcg ggcgtcgagg tcgaccccat
     2941 gcgcgatcag gcgctgcagc aaccgggtcc agtgcaggcg caccgccagt gccagcggcg
     3001 gatcgccggc gggcgaggcg ccatagggat cggcgccacg ctccagtagt tccagcgcga
     3061 agcgttgcgc gttcggtacc ggcgcctgcc caaggcacgc accgagccag gtcgcgaagc
     3121 cgccgccacc cgccggcgag ccgccggcgg cgaacaggac ccgcagggtg tcgatcccgt
     3181 ccgcgccgcg cgccagcgcc gccgccaccg gcgacggcgc ccccgggacc cgcgcctcgg
     3241 cctgcgcgcc gtggcgcagc agccattcga cccgcgccgg tgcggcggca acctcggcat
     3301 cgcacaccaa tccgttcagt tccgcgctgc ccagcagttg cgccagcgcc gccagttcgg
     3361 acccgccggc gccttccagc aggcgctcgc gcagcagcag ttgcggcggg cgatcggccg
     3421 gcacgccatc gtcgtcatcg tccatcgcga tcggcagttt ccgttgcgga tccagcgcgg
     3481 cgaccagcgc ccagcgaccg ccggcggcgg ccacgtcgac cgcgctgcgg ccttcggcat
     3541 cctgcaactg cgcgtccacg ccgagcgcca gcaggcgctt gaccagcgcg atcgagggtg
     3601 cgtccgcggc caccgcgaga tgcagcgcag tcgcgcccgc gccatcgcag tcgcgcgggt
     3661 cggcatgcgc ttccagcagg gtttccagcg ccggcagggc ggcgttgcgg gcactttcca
     3721 gcagcggcgt gcggccctcg ccatcacgcg cgtggacgtc ggcgccggca tccagcaggg
     3781 cggcgacgat ccccgaatgg ccggcgaatg cggcttcgtg cagggcgctg cggccgcggc
     3841 cgtcgcgtgc atccacccgc gccttgtgcc gcagcagcag ctgcacgccg gcggcatcgt
     3901 cctcttcggt gccggccgcg gccagcagca ccggctggcc gcccgtgggt tccggcctgg
     3961 cgccgcgttc cagcaggaag cgcgccagcc gccagttgcc ggcgctgcag gcgatgccga
     4021 gcggcgatac cccttcgccg ttcagcgcat ccacgtcggc gccggcgtcg cgcagcaagg
     4081 ccgccacgcc gggatcggag ctgcgcgcgg cgtggtgcag cggggtattg ccatcggcgt
     4141 cggtggcgcg cgggtcggcg ccattggtga gcagggtcat caccgcttcc ggccggccgt
     4201 gccagctgtc gcgggtggcg gcgagcagtg gcgtggtcgc ggcctgcgcg gcgttgaggt
     4261 ccaccccgcg ggcgatcagc gcacgcagca ggcgcaggtc gggcagcacc gccgccagca
     4321 ccggcagccc acggcggtcg cggtcgccgg cgggtggcgg ggcctgcgca tcggcgcctg
     4381 cttcgagcag ctgcagcgcg cgttcgaccc gtcccgcccg cgccaccgca tacagttcgc
     4441 gggtccgtgc ctcgccatcg accaccgccc cggccgccac cggcggcggc ggttcgtgcg
     4501 cgggggtggc ggcctccgct tcggcttcgg cctcctccat ctccaccacc ggcagcccgt
     4561 gcaccagcag ccgcgacggc gccgactgca gcagcgcgtg cgccagcggc agcaacgcgg
     4621 cataggcgcc ggtggcgacg gtgcgggcga aacccgccag caggccgggc caggccaggg
     4681 cgatcccccc acccagcagc agcagcgccg gtaccgcgac ctgcaacccg ctccaggctc
     4741 cgcgttcgtg cgctgcctgc gcggcgaagc gcgcgcggat gccgccgcct tcgcgttcaa
     4801 gcccatgcca cagcggccag tgccgccaca gcagcagcaa tgccaccgca ctggcggcgc
     4861 tcatcaccag cgtcggcagc agtcccggcg aggcccgcaa cgcctgcagc ggccatgccg
     4921 ccagcaggac cgcgaagatg aagcccgcgg cccacagcag cgccagcgcg agtgcatcgc
     4981 ggccgccgcg cgcgctgccg cgggtcccgc gcagcagggc gataccgagc gcggccggca
     5041 cctgcgccag ccaggccagt gcaccggcgg tgccgacatc gaaccagcag gcggcggcca
     5101 gcagcatcga cagcacgacc agcgcgaacc ccgggccgcg gcgtggctca ggcatcggtg
     5161 ccccagtcct cggtcatcgg atccagcatg gtcggcggga actgcaggtg gaagccgcgg
     5221 ccggcaaggt tggcgcggac cacgtcggga ttttcgcgcg ccagcgtgcg tcccggcgcc
     5281 aggtccacgt ccagcacgaa ctgcagcgca cccagttgcg tgcgcagcgg ctccggcagg
     5341 cgggcgaaat cgtcgcgtgc cgccaggtag acgtaggtgt cggccttttt caggctcttg
     5401 tagacgaaag caagcatcgc gcatcccgcg aaagaccgcc tgattgtgcg ggaaagcgcg
     5461 gtgcgacgga accgccagcg cgaccttcgg cacaagcgcc ggcgcgcgcg gctgctaccc
     5521 tgcggccgac ctcgatcccg gaccgcacga tgcgcctcgc cccgcttgcc gccgccctgt
     5581 tgctcacttc cctgtccgca acggccgccg agccgctgcg cctggccgag gcgatggccg
     5641 atccggactg gatcggcccg ccggtggaag acgcgtggtg gcgctgggac ggcaaggccg
     5701 cgcagtacat cctgaagcgc aagggcacgc ggatcggcga cacctggcag gtcggcatcg
     5761 acggcggcgc gccggccatg ctcgaaggca gtgcgcgcgc cgacctcgac gcggccagcg
     5821 cactcctgga caccagcggc agccgcagcg cgttcgtccg caacggcgac gtgttcgtgc
     5881 gcgacctgcg cagcggcgcg ctgacccaac tgacccgcag caacgaagcc gaatcgcgcc
     5941 tgctgtggag cggcgatggc gcgctggcct ggcgcggcgc ggacgcgtgg taccgctggg
     6001 acggccgcgc caccgtgcag gcggcgcagg cgaaggcgga ggacgcaccg ggcacgccgc
     6061 ccaaaccgga cgacctgcgc gaccgccagc tgcgcctgat cgaaaccctg aagaacgacc
     6121 gcgccctgcg cgaggccgcg cgcgagcagg gcgaggcctg gcgcaaggcc gacccgaccc
     6181 gcgcagccgc gccggtgtac ctgggcgcgg gcgtggacat cgtcgattcc gcgctgtcgc
     6241 cggatggtcg ctggctgctg gtcgccacca ccgagaaggg cgccgccacc ggcaccgccg
     6301 gcaagatgcc gatgtacgtc accgaatccg gctacgagga attcgaggac gtgcgcaccc
     6361 gggtcggccg cgccgatccg ctgccgcaga agctgtggct ggccgaggtt gcaaccggca
     6421 aggtcagcga actcaagttc gacgccctgc ccggcatcgc cagcgatccg ctggcggcgc
     6481 tgcgcaaggc cgccggcaag gaggcgctga agggcaaccg cgcggtgcag gtcgccacca
     6541 gcggcgacaa cggcgatgcg ccggccctgc tgtggagcaa cgacagccgc aacgcagcgg
     6601 tgatggtgcg tgcgatcgac aacaaggatc gctggatcgc caccgtcgac cttgccgccg
     6661 cgaaactgca gccgcgccac cggctcaccg acccggcgtg gatcaactgg ggcttcaacg
     6721 acttcggctg gctggccgac ggcagcacgc tctggtacct cagcgaggaa agcggctact
     6781 cgcacctgta caccctcgat ggcggaagca gggcgcagct caccgacggc aagtgggaag
     6841 cctcgtcggt ggtgccggcg cgcgatggca gcgggttctg gttcctgtgc aaccgttcgc
     6901 ggccgggcga ttacgaagtg tgcaacgtgc cggcgcgcgg tggcgcggtg cgcgaagtca
     6961 ccgcgctgga tggcgtcgag agcttccgcc tgtcgcccga cggcgccagg ctgctggtgc
     7021 gcaattcctc tgcctacatg ccgccgcagg cctacgtggt cgatgcggcg ggcggccagg
     7081 cgaagcagtt gaccgatacc cgcacaccgg aattcaaggc gcgcgaatgg ctccagccgc
     7141 agatcgtgca ggtgccttcg aagcatggtg ccggcaccat ctggggcaag tactacggtc
     7201 cggcgacgat ggagcccggc cgcaagtatc cggtcgtgat gttcgtgcac ggtgccggct
     7261 acctgcagaa cgtcagcgcg cgctatccca attacttccg cgagcagatg ttccacaacc
     7321 tgctggtgca gcagggctac atcgtgctgg acctcgacta ccgcgcctcc gaaggctacg
     7381 gccgcgactg gcgcaccgcg atctatcgct ggatgggcaa gc
//
LOCUS       DAORUK010000103         7365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_11377_length_7365_cov_17.940492, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000103 DAORUK010000000
VERSION     DAORUK010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11377_length_7365_cov_17.940492"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PK818_07625"
     CDS             complement(<1..236)
                     /locus_tag="PK818_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQY82636.1"
                     /translation="MTTPTTFADLALAPALRAGLDALDYTTLTPIQAQALPAILQGRD
                     LIAQAPTGSGKTAAFGLGLLQRIDPAAGGTQALVL"
     gene            351..1445
                     /locus_tag="PK818_07630"
     CDS             351..1445
                     /locus_tag="PK818_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1615 domain-containing protein"
                     /protein_id="HQY82637.1"
                     /translation="MTHDPRRPRPFAWLALCLGIACLSACDVAPAPEDVRAELLRKLP
                     ATLHERDGWARDIQAAFAAQNLQPSSANLCAVLAVVEQESGYRADPPVPGLARIARAE
                     IDRRAERLRIPTFMVEAGLRFRSADGRTYAQRIQALRTEQELSALFEEMTRRVPMGRR
                     LFARFNPVRTGGPMQVRIDFAQRHLRDYPYPLDGSVRGEVFSRRGGLYFGIKHLLGYP
                     ADYQAPLYRFADFNAGWYASRNAAFQQAAAIASGTRLARDGDLLTPGAPIDRPGATEA
                     ALRAIAPQLGMDAAGIRAALASGDAAGFADTALYRRVFALADGKGAGRPVARARVPDI
                     ALASPKISRKLTTAWFASRVEARWKHCMAR"
     gene            complement(1455..1976)
                     /locus_tag="PK818_07635"
     CDS             complement(1455..1976)
                     /locus_tag="PK818_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82638.1"
                     /translation="MKHQHERSAAVDGPAGIRDKGGWFIYLFFGIHMLMFGLSGFVMA
                     YSADRPELTFLYMHGGIAIVVYTVFYLVIFGLDEVRWMLINAALGILGIYSQVGWILE
                     RFGKRIDDYRWQVHVIPFLYYVLYTFLLRQFLLDVTRSRDNPKRRAQVNNAYVVVSLL
                     VYGLVLWRMRDGG"
     gene            2134..3228
                     /locus_tag="PK818_07640"
     CDS             2134..3228
                     /locus_tag="PK818_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82639.1"
                     /translation="MSLRWIPLALCLAVVACARPPEEQTGASVDQGAQAAPAVQRKEQ
                     AGIDWSPTRLEAGDAWIDCGADYASGDGRPVLSLAYVDLREAMQDCRGTGLVRLRYQG
                     KVTTSFAALAERTGKVATDLGIGKRILDIDSAGGHVEAAIRAGDAIGENDWTIWVRGD
                     SSCHSACVLLLAAGDMRVVAGPVGIHRMIRVGSAATSRRELGEELRTISDNLRNYLER
                     NGASAEVYDLMTTVPNRTLRILAPGELEQYGLAGANAVEDDLNRIRLARRCGNDFVAR
                     RDAFRRAYDARCMQPEDEAAQDVVDINACGMALRRHFGFPDPQCPDESPMAELDGAIL
                     EIPHLAAEPEAIEAIEATDAAVVERTEAPR"
     gene            complement(3230..3385)
                     /locus_tag="PK818_07645"
     CDS             complement(3230..3385)
                     /locus_tag="PK818_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82640.1"
                     /translation="MDAKTAETAMKKILVGLAVVAILCMVGGAGYRFGQYLAERDAPV
                     RQANAGA"
     gene            complement(3483..4151)
                     /locus_tag="PK818_07650"
     CDS             complement(3483..4151)
                     /locus_tag="PK818_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HQY82641.1"
                     /translation="MDAIMELDEFKSAWQALDRRLELDNRLRLHDLRERTLHKTRGNL
                     RPLYWGQVAQTLFGLIFILLAGLLWMSHPAHASTIVAGVLVHAYGVLTIIGAGVVISV
                     IGKVDHARPVLDIQKQLLRIRTLYIRSGMVAGLPWWFLWIAILQVLAGLGDVDLLAKA
                     PSLVWSGYGIGIAGLLATWWFHRWARRPERGEFGRKMDDSLTGGSLRKALAQLEELKR
                     FEQE"
     gene            complement(4166..4669)
                     /locus_tag="PK818_07655"
     CDS             complement(4166..4669)
                     /locus_tag="PK818_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017914609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQY82642.1"
                     /translation="MTATDDRRDAFSALLQRHRGIVFKVANTYARLAEDRDDLAQEIA
                     ARLWHAYPKYDPARSFSTWMYRVALNVAISHVREQTMRDRHDAVPLDEGMHDLADAGG
                     VDHERDQHIRLLQGFIQRQPPLERALLLLYLEDRPQREIAEILGLTENNVSTKIGRLK
                     QRIRDEP"
     gene            complement(4770..4991)
                     /locus_tag="PK818_07660"
     CDS             complement(4770..4991)
                     /locus_tag="PK818_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HQY82643.1"
                     /translation="MQTLDFELDRDFVELNQLLKLAGLCDSGGAGKQLVASGAVRVDG
                     RPEDRKTAKIRAGQVVRVGDVEIRVVAAA"
     gene            5059..5433
                     /locus_tag="PK818_07665"
     CDS             5059..5433
                     /locus_tag="PK818_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQY82644.1"
                     /translation="MLAYATLGTRDLAKAAAFYDAVLAPLAAKRFMEEPDYFIAWGNS
                     EAGAGLGITYPFDKQAATVGNGCMVALGASSREQVDAVYAKAIELGGSDEGAPGQRYP
                     GFYAAYFRDLDGNKLNCCYMGE"
     gene            complement(5455..>5769)
                     /locus_tag="PK818_07670"
     CDS             complement(5455..>5769)
                     /locus_tag="PK818_07670"
                     /inference="COORDINATES: protein motif:HMM:NF027851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HQY82645.1"
                     /translation="PAAAPAPAPAPTAAIAQPAPPPARPLPPPMPAGTREVADIDATS
                     LPPVKLSMHMWDESPARRFVILDGQRMTEGDRSGGLSVVAIERDGVVVERNGQRARVP
                     LP"
     assembly_gap    5770..5869
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5870..6198)
                     /locus_tag="PK818_07675"
     CDS             complement(<5870..6198)
                     /locus_tag="PK818_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82646.1"
                     /translation="MSLILEALRKSEAERRRGSTPDVAMELPPVPAARARATPAWVLP
                     ALVIAIFALLAGGWWSQRDTGGDAMTQAAAPAVAEPLVDTPAPPVVVARAETPAAPPA
                     TPAPMPPA"
     gene            complement(6203..>7365)
                     /locus_tag="PK818_07680"
     CDS             complement(6203..>7365)
                     /locus_tag="PK818_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HQY82647.1"
                     /translation="LLTNLETDTQKLLQILLLGQPELRDMVARPELRQLAQRITARYH
                     LTPLDAKETGEYLRHRWRVAGGQKFPFDAKAVQRMHQRSGGVPRLLNVIAERALLAGY
                     ARDATVIDARLVDAAANEVLQPAARQPWWPWLATAAGLAAIALLAMQWRDRPLPTIEA
                     GNTVAAASTNAPQPATTPAATRVDVLDAAGLTQLIGATGDTPVPAWQHLLGLWALPAG
                     SITVDPAGPCTPASAGDVHCVQGRAKLGSLLALDRPQLLRLQDGDASAWALLLGADSR
                     KLRLRIGTRTFDLDRVLLQSRWTGDYAGLWRGPAMLGKPPRMGDAGPAVDWLRQRLAA
                     GAPGTAYDDALRAHVTTLQDARGLPADGIAGPLTLMALADDPPGPRLLRVLD"
BASE COUNT         1063 a         2522 c         2597 g         1083 t
ORIGIN      
        1 agcaccagcg cctgggtgcc cccggccgcg gggtcgatcc tttgcagcag gcccagcccg
       61 aatgccgcgg tcttgccgct gccggtgggc gcctgcgcga tgaggtcgcg gccttgcagg
      121 atcgcgggca gcgcctgcgc ctggatgggg gtgagggtgg tgtaatcgag ggcgtcgagg
      181 ccggcgcgca gcgcgggcgc aagggcaagg tcggcgaacg tggtgggcgt ggtcatcgcc
      241 catgatcgca ggcggagcgc gcttgcagtg gatttccggt cgagcgcgcg cactcggccg
      301 cggttcagca cggactgcta gcctctgcgc gaaatcgccg gcagcgccgc atgacgcacg
      361 atccccgacg tccccgacct ttcgcgtggc tcgccctttg cctggggatc gcctgcctgt
      421 ccgcctgcga cgtggcgcct gctccggagg acgtgcgcgc cgaactgctg cgcaagctgc
      481 cggccaccct gcacgagcgc gatggctggg cgcgcgacat ccaggccgcg ttcgcggcgc
      541 agaacctgca gccgagcagc gccaacctgt gcgcggtgct ggcggtggtc gagcaggagt
      601 cgggctatcg cgccgacccg ccggtgccgg gcctggccag gatcgcgcgg gcggagatcg
      661 accgccgcgc ggaacggctg cggatcccga cgttcatggt cgaggccggg ttgcgcttcc
      721 gttcggccga tggccgcacc tatgcgcaac gcatccaggc attgcgcacc gagcaggagc
      781 tgtccgcgtt gttcgaggag atgacccgca gggtgccgat gggccggcgc ctgttcgcgc
      841 gcttcaaccc ggtgcggacc ggcgggccga tgcaggtgcg catcgatttc gcccagcgcc
      901 acctgcgcga ttacccgtat ccgctcgatg gctccgtgcg cggcgaggtg ttcagccggc
      961 gcggcggtct gtacttcggg atcaagcacc tgctcggcta tccggccgac taccaggcgc
     1021 cgctgtaccg gttcgcggat ttcaatgccg gctggtatgc aagccgcaat gcggcattcc
     1081 agcaggcggc ggcgatcgcc agcggcaccc gcctcgcgcg cgatggtgac ctgctgacgc
     1141 ccggcgcgcc gatcgaccgg cccggcgcga ccgaggccgc gttgcgcgcg atcgcgccgc
     1201 agctggggat ggatgccgcc ggcatccgcg cggcgttggc cagtggtgac gccgccgggt
     1261 tcgcggacac cgcgctgtac cggcgggtgt tcgcgcttgc cgacggcaag ggcgcaggca
     1321 ggccggtggc gcgcgcgcgg gtgccggaca tcgcgctggc aagcccgaag atcagccgca
     1381 agctgaccac cgcgtggttc gccagccggg tcgaggcgcg ctggaagcac tgcatggcgc
     1441 gctgagcgcg tgcctcagcc gccgtcgcgc atgcgccaca ggacaaggcc gtacaccagc
     1501 agcgagacca ccacgtaggc gttgttgacc tgcgcgcggc gcttcgggtt gtcgcggctg
     1561 cgggtcacgt ccagcaggaa ctggcgcagc aaaaaggtgt acagcacgta gtacaggaac
     1621 gggatgacgt ggacctgcca ccgatagtcg tcgatgcgct tgccgaagcg ttccaggatc
     1681 cagccgactt gcgagtagat gccgaggatg ccgagcgcgg cgttgatcag catccagcgg
     1741 acctcgtcga ggccgaagat caccaggtag aacaccgtgt acaccacgat ggcgatgccg
     1801 ccgtgcatgt acaggaaggt gagctcgggc cggtccgccg aatacgccat cacgaagccg
     1861 gacaggccga acatcagcat gtggatgccg aagaacaggt agatgaacca gccgcccttg
     1921 tcgcgaatgc ccgccgggcc gtccacggcc gcggaccgct cgtgctggtg cttcatgcgt
     1981 gtctccccga tgactcgttg ctgcaaacgc agcccgcggc cgcagccggc cgcgtcgtca
     2041 cgacgcaagg ccatgatcgc gattcatcgc tggttgggca gcccgttgcc gccgcgcact
     2101 agactgcccg caaatccccg accggttcgc ccgatgtcgc ttcgttggat cccgcttgcg
     2161 ctgtgcctgg ccgtggtcgc ctgtgctcgc ccgcccgagg aacagaccgg cgccagcgtc
     2221 gaccagggcg cgcaggcggc accggcggtg cagcgcaagg agcaggccgg gatcgactgg
     2281 tcgccgaccc ggctggaagc gggcgacgcc tggatcgact gcggcgccga ctacgccagt
     2341 ggcgacggcc ggccggtgct gtcgctggcc tacgtcgacc tgcgcgaagc gatgcaggac
     2401 tgccgcggca ccgggctggt gcggctgcgt taccagggca aggtcaccac cagtttcgcc
     2461 gcgctggccg aacgcaccgg caaggtcgcc accgacctcg gcatcggcaa gcgcatcctc
     2521 gacatcgatt ccgccggcgg ccacgtggaa gccgcgatcc gcgccggcga cgccatcggc
     2581 gagaacgact ggacgatctg ggtgcgcggc gactcgtcct gccacagcgc ctgcgtgctg
     2641 ctgctggccg ccggcgacat gcgcgtggtg gccgggccgg tcggcatcca ccgcatgatc
     2701 cgggtcggct cggcggcgac ctcgcgcagg gaactgggcg aggagttgcg tacgatcagc
     2761 gacaacctgc gcaactacct ggagcgcaac ggcgcatccg ccgaggtcta cgacctgatg
     2821 accaccgtgc ccaaccgcac cttgcggatc ctcgcccccg gcgagcttga gcagtacggg
     2881 ctggcgggcg cgaacgcggt ggaagacgac ctcaaccgca tccgcctggc gcgccgctgc
     2941 ggcaacgact tcgtggcccg ccgcgacgca ttccgccgcg cctacgacgc ccgctgcatg
     3001 cagccggagg acgaggccgc gcaggacgtg gtcgacatca atgcctgcgg catggccctg
     3061 cgccgccact tcggcttccc cgacccgcaa tgcccggacg aaagcccgat ggcggaactc
     3121 gatggcgcga tcctcgagat tccccatctg gctgccgagc cggaggcgat cgaggcgatc
     3181 gaggcgacgg atgcggcggt cgtggaacgt accgaagcgc cgcgttgatt caggcgccgg
     3241 cattcgcctg tcgaacgggt gcgtcccgct ccgccaggta ctggccgaag cggtagccgg
     3301 cgccgccaac catgcacagg atggcgacca cggccaggcc aaccaggatc ttcttcatcg
     3361 cggtttccgc cgtcttcgcg tccatggcgt cacgctagtc gcgcccgtga tgcttgcaac
     3421 gtgcccgaag ccagccaact tccagcaggg cggcgatgcc ggccggcatc gccgcgcgcg
     3481 gctcattcct gctcgaagcg cttgagttcc tcgagctggg ccagcgcctt gcgcaggctg
     3541 ccgccggtca ggctgtcgtc catcttgcgg ccgaactcgc cgcgttcggg gcgtcgcgcc
     3601 cagcggtgga accaccatgt cgccagcagg ccggcgatgc cgatgccgta gccgctccac
     3661 accagcgacg gcgccttcgc cagcaggtcg acatcgccca ggccggccag cacctgcagg
     3721 atcgcgatcc acaggaacca ccacggcagg ccggcgacca tcccgctgcg gatgtacagc
     3781 gtgcgaatgc gcagcaactg cttctggatg tcgagcaccg gcctcgcatg atcgaccttg
     3841 ccgatcacgc tgatcaccac gccggcgccg atgatggtca gcacgccata ggcatgcacc
     3901 agcacgccag cgacaatcgt ggaggcatgc gcggggtggc tcatccacag cagcccggcg
     3961 agcaggatga agatcaggcc gaacagggtc tgtgcgacct ggccccagta cagcgggcgc
     4021 aggttgccgc gcgtcttgtg cagggtgcgc tcgcgcaggt cgtgcagcct gaggcggttg
     4081 tccagttcca ggcggcggtc gagcgcctgc caggcggatt tgaattcgtc gagttccatg
     4141 atggcgtcca tgtggtcctg tgcggtcagg gttcgtcgcg gatgcgctgc ttgaggcgtc
     4201 cgatcttggt ggagacattg ttttcggtga ggccgaggat ctccgcgatt tcacgttgcg
     4261 ggcgatcttc gagatagagc aggagcaagg cgcgctccag tggcggctgc cgctggatga
     4321 agccctgcag caggcggatg tgctggtcgc gctcgtggtc cacgccgccg gcatcggcga
     4381 ggtcgtgcat gccttcgtcc aatggcaccg cgtcatggcg gtcgcgcatg gtttgctcac
     4441 gcacatggct gatcgccacg ttcagcgcca cccggtacat ccaggtgctg aagctgcgcg
     4501 ccgggtcgta cttcgggtat gcgtgccaga gccgggccgc gatctcctgc gccaggtcgt
     4561 cgcggtcctc ggccagccgc gcataggtat tggcgacctt gaacacgatc ccgcgatggc
     4621 gctgcagcag ggcgctgaag gcgtcgcggc gatcgtctgt cgcggtcatg gcagcgatgg
     4681 tatccatggc atccccggcg cgttgcgcgc gcctgtcgga accatgattc gcaccggcgc
     4741 gcggaacctc acaccacccc gcgaaaaaat caggcggcgg ccaccacccg gatctcgaca
     4801 tcgcccacgc gcaccacctg gccggcgcgg atcttcgcgg tcttgcggtc ctcgggcctg
     4861 ccgtccaccc gcaccgcgcc gctggccacc agctgcttgc cggcgccgcc ggagtcgcac
     4921 aggccggcca gcttcagcag ctggttgagt tcgacgaagt cgcggtcgag ttcgaaatcg
     4981 agggtctgca tggatcgagc ataccggcca aggcgcggcg ggtgtaggct gccgctcccg
     5041 cccacaggag accttcccat gcttgcctat gccaccctcg gtacccgcga cctggccaag
     5101 gccgccgcct tctacgatgc ggtgctggcg ccactcgccg ccaagcgatt catggaggag
     5161 ccggactact tcatcgcctg gggcaactcg gaagccggtg ccgggctcgg catcacctat
     5221 ccgttcgaca agcaggccgc cacggtcggc aatggctgca tggtggcgct cggcgccagc
     5281 agccgcgaac aggtggatgc ggtgtacgcc aaggcgatcg agctgggcgg cagcgatgaa
     5341 ggcgcgcccg ggcagcgtta ccccggcttc tacgcggcct atttccgcga cctggacggc
     5401 aacaagctca actgctgcta catgggcgaa taagccgccg cggcgcagcg ccgatcaggg
     5461 caacggcacg cgcgcgcgct ggccgttgcg ctccaccacc acgccatcgc gttcgatcgc
     5521 gaccacgctg agcccgccgc tgcggtcgcc ctcggtcatg cgctggccgt cgaggatgac
     5581 gaagcgccgc gctggcgatt cgtcccacat gtgcatgctc agcttcaccg gcggcagcga
     5641 ggtcgcgtcg atgtcggcga cttcgcgcgt ccctgccggc atcggcggtg gcagcgggcg
     5701 ggccggcggc ggcgcgggtt gggcgatggc cgcggtgggc gcgggtgccg gagccggagc
     5761 ggcggccggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ccggtggcat
     5881 cggtgccggg gttgctggcg gggcggctgg tgtctccgcg cgtgccacga ccaccggcgg
     5941 tgcgggcgta tcgaccaggg gttccgcgac ggccggtgca gcggcctggg tcatggcatc
     6001 gccgccggtg tcgcgttgcg accaccagcc gccggcgagc aacgcgaaga tcgcgatgac
     6061 caaggcgggc agcacccagg ccggcgtggc gcgcgcacgc gccgcgggta ccggcggcag
     6121 ttccatcgcc acgtccggcg tgctgccgcg gcggcgctcg gcttcggatt tgcgcagggc
     6181 ttcgaggatc agcgacatcg gctcagtcca gtacgcgcag caggcgcggg ccgggtggat
     6241 cgtcggccag cgccatcagg gtcagcggcc ccgcgatgcc gtccgccggc aggccgcgcg
     6301 cgtcctgcag ggtggtgacg tgcgcgcgca gcgcgtcgtc gtaggcggtt cccggcgcgc
     6361 ctgccgcaag ccgctggcgc agccagtcca ccgccgggcc ggcatcgccc atgcgcggcg
     6421 gtttgcccag catcgccggt ccgcgccaga ggccggcgta gtcgccggtc cagcgcgatt
     6481 gcagcagcac gcggtcgagg tcgaaggtgc gggtaccgat gcgcaggcgc agcttgcgcg
     6541 aatccgcgcc cagcagcagc gcccatgcgc tggcatcgcc atcctgcagg cgcagcagtt
     6601 gcggacggtc cagcgccagc aggctgccca gcttcgcgcg cccctgcacg cagtgcacgt
     6661 cgcccgcgga ggccggcgtg cacgggcccg cgggatccac ggtgatgctg cccgcgggta
     6721 gcgcccacaa cccgagcagg tgctgccacg cgggcaccgg ggtgtcgccg gtggcgccga
     6781 tcagctgggt gagtccggcg gcatcgagca cgtcgacgcg cgtggccgcc ggtgtcgtgg
     6841 caggttgcgg tgcgttggtc gacgccgccg cgaccgtgtt gcccgcttca atcgtcggta
     6901 acggacggtc ccgccactgc atcgccagca gcgcgatcgc cgccaggccc gccgccgtgg
     6961 ccagccacgg ccaccagggt tgcctggcgg cgggttgcag cacttcattg gcggcggcat
     7021 ccaccagcct ggcatcgatc acggtcgcat cgcgcgcata gccggccagc agcgcgcgtt
     7081 cggcgatcac attgagcagg cgcggcaccc cgccggagcg ctggtgcatg cgctgcaccg
     7141 ccttcgcgtc gaacgggaat ttctgcccgc cggccacgcg ccagcggtgg cgcaggtact
     7201 cgccggtttc cttggcgtcg agcggggtca ggtggtagcg cgcggtgatg cgctgggcca
     7261 gctggcgcag ttccggcctg gccaccatgt cgcgcagttc cggctggccc agcagcagga
     7321 tctgcagcag cttctgggta tcggtttcca ggttggtgag caggc
//
LOCUS       DAORUK010000104         7353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_11413_length_7353_cov_18.297342, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000104 DAORUK010000000
VERSION     DAORUK010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11413_length_7353_cov_18.297342"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..690
                     /locus_tag="PK818_07685"
     CDS             202..690
                     /locus_tag="PK818_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HQY82648.1"
                     /translation="MSKVLARCVPLPKLSRFGWLMGLYAENHARLQRLFEPADLACGG
                     YVSSIGDGLDLRIDVLARHAYTVELRMSYGLVDPQTGLFDPSANLRLYNDARQVEATH
                     CYAGRRWQDVIGMFPPPERILDHRLRMNTFLGKWLQYLAEQGHGVATLRPASQVGIET
                     AA"
     gene            732..807
                     /locus_tag="PK818_07690"
     tRNA            732..807
                     /locus_tag="PK818_07690"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:765..767,aa:Ala,seq:ggc)
     gene            855..930
                     /locus_tag="PK818_07695"
     tRNA            855..930
                     /locus_tag="PK818_07695"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:889..891,aa:Glu,seq:ttc)
     gene            complement(1045..1680)
                     /locus_tag="PK818_07700"
     CDS             complement(1045..1680)
                     /locus_tag="PK818_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82649.1"
                     /translation="MTIRSHITVLLDRTGSMDTIRDDVIGGFNAFLAQQQAAPEPGTM
                     TLVQFDSQDPYEVIADFAPLAAIAPLTAETYIPRATTPLYDALGRGILELEARLAAIP
                     DALRPGRILYVVVTDGQENASREFDRARVSTLINAKQALGWEFIFLSADLAAFDDAQS
                     LGIGRNSSLLFGKHKRGSDVAWSLASGKIRERRAGISDAVAFDEGERKSAE"
     gene            1772..4174
                     /locus_tag="PK818_07705"
     CDS             1772..4174
                     /locus_tag="PK818_07705"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQY82650.1"
                     /translation="MTQLARLEVQPAAADGGSSSSLAPRLLRKYPERVTGAFVVPARE
                     GCFVALPDALPERLAAALRSRGRERLYSHQGACWDAVARGEDVVVVTPTASGKSLCYT
                     LPVVAGAMTRRSKALYLFPTKALAQDQVAELIELSKAGELGVRAATFDGDTPGDQRQA
                     IRLSGDIVVTNPDMLHQGILPHHTKWAQFFENLAYVVIDEIHAYRGVFGSHLANVLRR
                     LQRVCAFYGVRPQFILCSATIGNPQQHAEALIGRPVTAITESGAPSGEKHVLLWNPPV
                     VNPDLGLRASARSQSNRIARMAIKAGLKTLVFAQSRTMVEVLTKYLKDVFDHDPRKPA
                     RIRAYRGGYLPTERRDAERAMRDGRIDGIVSTSALELGVDIGALDVVVLNGYPGSVSG
                     TWQRFGRAGRRQKPSLGVLVASSDPLDQYIVRHPEFFSDASPEHARIAADQPVILLDH
                     IRCAAFELPFLEGDSFGPVDPAVYLQLLAEDGVLHPEGGRWEWIADSYPAIAVNLRSV
                     ADGNFVVVDRTDGRQTIIAEVDFSSAPLMLYEGAIHMVQSTPYQVERLDWEGRKAFVT
                     RTRVDYYTDAIDYTRLKVLESFAQAVTGVGQVHHGEVHVVRRVSGYKKIRYYTHENIG
                     YGPVTLPDQEMHTSACWWQLPELVLDALFESRQHALDGFLGAAHALHVVARIAVMADG
                     ADLRKAVGSGDGAWSASGDGHGRGQLRATDGAPLPAGALARFQPTVYLYDNYPGGVGL
                     SEPLFARQAELLLRAQALVQDCGCRAGCPSCVGPVLDDAVPLDEGPRQLALRVLRALQ
                     SA"
     gene            4324..4980
                     /locus_tag="PK818_07710"
                     /pseudo
     CDS             4324..4980
                     /locus_tag="PK818_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007973071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
     gene            5134..5499
                     /locus_tag="PK818_07715"
     CDS             5134..5499
                     /locus_tag="PK818_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HQY82651.1"
                     /translation="MLLLLAAFAAGAGELHRCVSRTGQVSYLSASCPDGQRTDRILQF
                     VPDPVPAPVTTRTGKASASGNRQSSRQRTVASHKPEPSPCQRAKARREAQLDQLGLKR
                     TFDDLSRIDAAVRVVCNGF"
     gene            complement(5569..5967)
                     /gene="crcB"
                     /locus_tag="PK818_07720"
     CDS             complement(5569..5967)
                     /gene="crcB"
                     /locus_tag="PK818_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HQY82652.1"
                     /translation="MNLWWQQLALVMLGGALGAGARYWLGGAMLRQFGTGIPYGTLAA
                     NLVGSFAVGFLAVWLEGRGPHALYWRAFLIVGVLGALTTYSALMLECLLMARSQRSDL
                     LLGYLGATLVGGFMLVWAGARLAGVLRPVY"
     gene            complement(5964..7325)
                     /locus_tag="PK818_07725"
     CDS             complement(5964..7325)
                     /locus_tag="PK818_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HQY82653.1"
                     /translation="MPAPPKQADLPELQDAASASLRPLAERMRPRALADMVGQRRLLA
                     PGSALRRAVEGGHVHSMILWGPPGCGKTTLALLLARHADAEFVAISAVLSGLPQVREV
                     LAQAQARFQAGRRTVLFVDEVHRFNKAQQDAFLPHIERGTILFVGATTENPSFELNSA
                     LLSRCRVHVLEAVSADDIALALRHALDDAEHGLGGRCLEVADAALREIAGAADGDVRR
                     ALTLLEIAADLADGEGGCISEATLHQVLADRTRRFDKGGDQSYDQISALHKCVRSSNP
                     DAALYWLARMLDGGADPGYLARRLTRMAVEDIGLADPRALQMAIEAWNAYDRLGSPEG
                     ELALAQLTLYLASTAKSNAAYRAFNAARADVEAHGTQDVPLHLRNAPTRLMKSLGFGK
                     DYQYDHDVDGGIALDQTGFPDAMGERVYYQPVQRGMEGKLKEKLDTLRAARTQVRKQK
                     NRP"
BASE COUNT         1121 a         2493 c         2571 g         1168 t
ORIGIN      
        1 tgcggcggga gggcatcgag atgctgagca ccacgcatgc atcgatcgag gagatttcca
       61 gccgcgtcct cgagtacctc ggcatcaatc gcgagatgta ctgagccggt ttttcggtcg
      121 gcacgggaac cccacgttag gccgcgcgca acgcgccgtc aatgctgccg cgacgcggat
      181 cgcgcgtgta ggatcggggc catgagcaag gtcctggccc gctgcgtccc gctcccgaag
      241 ctcagccgct tcggctggct gatgggcctg tacgcggaga accacgcgcg cctgcaacgc
      301 ctgttcgaac cggccgacct ggcctgcggc ggatacgtct ccagcatcgg cgacggcctg
      361 gacctgcgga tcgacgtgct ggcgcgccac gcctacacgg tggaactgcg gatgagctac
      421 ggcctggtcg atccgcagac cggcctgttc gacccgtccg ccaacctgcg cctgtacaac
      481 gacgcgcgcc aggtcgaagc cacccattgc tacgccggcc gcagatggca ggacgtgatc
      541 ggcatgttcc cgccgccgga gcggatcctc gaccaccggc tgcggatgaa caccttcctc
      601 ggcaagtggc tgcaatacct ggccgagcag ggccacggcg tggcgacgtt gcggccggca
      661 tcgcaggttg gcatcgaaac agccgcttga cgcatccggg attcacccgc aaaatgcgcg
      721 tcccgtttca tggggccata gctcagctgg gagagcgcct gcatggcatg caggaggtcg
      781 gcggttcgat cccgcctggc tccaccaatt cgcgaaccga caagcctcgt tggcaagcga
      841 atcaagtttc atgtgtcccc atcgtctaga ggcctaggac accacccttt cacggtggac
      901 accggggttc gaatccccgt ggggacgcca tccacagcgc aacacgaaaa ccgccgcaag
      961 gcggtttttt gttttccgca tttcctcgcc tgcagagcgg ggcatgcgca agtgtcgctt
     1021 taccacggag caggtcaaaa gacctcactc cgccgacttg cgttcgcctt cgtcgaacgc
     1081 caccgcatcg gagatgccgg cgcgacgctc gcggatcttg cctgaggcca gcgaccaggc
     1141 gacgtcgctg ccgcgcttgt gcttgccgaa cagcaggctc gagttgcggc cgattccaag
     1201 cgactgcgcg tcgtcgaatg ccgccaggtc agcgctcagg aagatgaatt cccagcccaa
     1261 tgcctgcttg gcgttgatca gggtactcac gcgcgcgcgg tcgaattcgc ggctggcgtt
     1321 ttcctggccg tcggtcacca ccacgtacag gatcctgccc gggcgcaggg cgtccggaat
     1381 cgccgccagg cgcgcctcca gctcaaggat gccgcggccc agcgcgtcat acagcggcgt
     1441 ggtggcgcgc gggatgtagg tctcggcggt gagcggcgcg atggcggcca gcggcgcgaa
     1501 atcggcgatc acttcgtacg ggtcctgcga atcgaactgg accagggtca tcgtgcccgg
     1561 ctcgggtgcg gcctgctgct gggccaggaa ggcgttgaac ccgccgatca cgtcgtcgcg
     1621 gatggtgtcc atggaaccgg tgcggtcgag caggacggtg atgtggctgc gaatggtcat
     1681 ggcggcgcct cctggagtga gccgccaacg tgcaccatgc gcgtcgcagc cggtgagaca
     1741 tgggcgccgg atcctcgcgg gcggaggatc catgacccag cttgcacgac tcgaagtcca
     1801 gccggccgcc gctgatggcg gctcttcgtc ttcactggcg ccgcgcctgc tgcgcaagta
     1861 tccggagcgt gtcaccggcg cgttcgtggt cccggcgcgg gagggatgct tcgtcgcgtt
     1921 gccggatgcg ctgccggaga ggttggccgc tgccttgcgc agccgcgggc gcgagcgcct
     1981 gtattcgcac cagggtgcgt gctgggatgc ggtggcgcgc ggcgaggatg tggtggtggt
     2041 cacccccacc gcgtctggca agtccctgtg ctacacgctg ccggtggtcg cgggggcgat
     2101 gacccgccgc agcaaggcgc tctacctgtt cccgaccaag gcgctggcgc aggaccaggt
     2161 ggcggagctg atcgagctgt cgaaggcggg cgagctgggc gtgcgcgcgg ccaccttcga
     2221 tggcgacacc cccggcgacc agcggcaggc gatccgcctg tccggcgaca tcgtcgtcac
     2281 caatccggac atgctgcacc agggcatcct gccgcaccac accaagtggg cgcagttctt
     2341 cgagaacctg gcctacgtgg tgatcgacga gatccacgcc taccgcgggg tgtttggctc
     2401 gcacctggcc aacgtgctgc gccggctgca acgggtctgc gcgttctatg gcgtacgccc
     2461 gcagttcatc ctgtgctcgg ccaccatcgg caacccgcag cagcatgccg aagccctgat
     2521 cggccgcccg gtcaccgcga tcaccgagtc cggcgcgccg tccggcgaga agcacgtgct
     2581 gctgtggaat ccgccggtgg tgaacccgga cctgggcctg cgcgcatcgg cgcgctcgca
     2641 gtccaaccgg atcgcgcgga tggcgatcaa ggccgggttg aagacgctgg tattcgcgca
     2701 gtcgcggacg atggtggagg tgctgaccaa atacctgaag gacgtgttcg accacgatcc
     2761 gcgcaagccg gcgcgcatcc gcgcctaccg cggcggctac ctgcccaccg agcgccgcga
     2821 tgccgagcgc gcgatgcgcg acggccgcat cgacggcatc gtctccacct cggcgctgga
     2881 actgggcgtg gacatcggcg cgctggacgt ggtggtgctc aacggctacc cgggcagcgt
     2941 cagcggcacc tggcagcgct tcgggcgtgc ggggcggcgc cagaagccca gcctgggcgt
     3001 gctggtggcg tcttcggatc cgctggacca gtacatcgtg cgccacccgg agttcttttc
     3061 cgacgccagt ccggaacatg cccgcattgc cgccgaccag ccggtgatcc tgctggacca
     3121 catccgctgc gctgcgttcg agctgccgtt cctggaaggc gacagcttcg gcccggtgga
     3181 cccggcggtg tacctgcagc tgctggccga ggatggcgtg ctgcatccgg aaggcgggcg
     3241 ctgggagtgg atcgccgaca gctatcccgc catcgcggtc aacctgcgct cggtggcgga
     3301 tggcaacttc gtggtggtgg atcgcaccga cggccgccag acgatcatcg ccgaagtgga
     3361 tttcagctcc gcgccgctga tgctctacga gggcgcgatc cacatggtgc agtccacgcc
     3421 ataccaggtg gagcggctgg actgggaggg ccgcaaggcg ttcgtcaccc gcacccgcgt
     3481 ggactactac accgatgcga tcgactacac ccggctgaag gtgctggagt cgttcgcgca
     3541 ggcggtgacc ggggtggggc aggtccacca cggcgaggtc cacgtggtgc ggcgggtctc
     3601 gggctacaag aagatccgct actacacgca cgagaacatc ggctacgggc cggtcacctt
     3661 gccggaccag gaaatgcaca ccagcgcgtg ctggtggcag ttgccggagc tggtgctgga
     3721 tgcattgttc gaatcgcgcc agcacgcgct ggacgggttc ctcggcgccg cgcatgcgct
     3781 gcatgtggtc gcacggatcg cggtgatggc cgatggcgcc gacctgcgca aggcggtggg
     3841 cagcggcgac ggggcgtggt ctgccagcgg tgatggccac gggcgtggcc agctgcgggc
     3901 caccgatggc gcgccgctgc cggccggggc gttggcgcgt ttccagccaa cggtttacct
     3961 gtacgacaac tacccgggcg gggtggggct gtcggagcca ctgttcgcgc gccaggccga
     4021 actgttgctg cgggcgcagg cgctggtgca ggactgcggc tgccgggcgg gatgcccgag
     4081 ctgtgtcggc ccggtgctgg atgatgcggt gccgctggac gaaggcccgc gccaactcgc
     4141 gttgcgggtg ctgcgcgcgc tgcagtccgc atgagcgttg gcctcgaccg cctgcaggcg
     4201 ctgcggcggc agagcggggc ggcagcgccg cgtgcgccgg cggttgcgct gcccgaaccc
     4261 gtggatgcgc tggtcgagca ggtcgcccgc ctgcgtggcc tgttgcgggt gacgcgcggt
     4321 agcgcgccgt tggtggccgc cagtgcggac cgcacgattc ccggccgcga aatcgccgcg
     4381 ggcctgtggc tgcaggaaca gcggctgccg ctcggggatc catggcccgt gctgcttgat
     4441 gccggcttcg atcgccgcga gccggtgccc acccacgacc tgttgttctt cgataccgaa
     4501 accaccggcc ttgccggcgg caccggcacc cgcgcgttca tgatcggcgc tgccgcgatc
     4561 gacggcgacg cgctgcacgt gcgccagttg acgatcgcca gcaccggtgc cgaagcggcg
     4621 atgctggagg tgttccgcgg ctggctgcgc gcctccaccg ttttggtcag ctacaacggc
     4681 aagtcgtacg acgcgccgct gctgaagacg cgctaccggc tgatgcgcca ggccgaaccg
     4741 ctctcgggcc ttgcgcacat ggatctgctg taccccagcc gccgccgcta ccgcggctgc
     4801 ttcgagaact gccggctcgc caccatcgag cggcgggtgc tcggcatcgt gcgcgaggac
     4861 gacctgcccg gcagcgaggc cccggccgcg tggctcggct acctgcgcgg cggttccggc
     4921 gcgctgctgc ggcgggtgct ggcgcacaac cggcaggacg tggtgacgct ggcgcggttg
     4981 gccctgcacc tgggcagcgc gtgagtcgat gcgggcggtt tcccgacgga ccgacgcgtg
     5041 taaccccggc tgcgttccgt cgggccacgc ggcagcatct ccagcatcaa ccccgatgcg
     5101 tgaaggccga tgagacaccg ttcgctgccg tgcttgctgc tcctgctggc tgcgttcgcc
     5161 gcgggtgccg gcgaattgca tcgctgcgtc agccgcaccg gccaggtcag ctatctctct
     5221 gcgagctgcc cggacggcca gaggacggat cgcatcctgc agttcgtccc ggatccggtt
     5281 cctgcgcccg ttaccacgcg caccggcaag gcgtcggcat ccggaaaccg ccagtcgtct
     5341 cgccaacgaa ctgttgcgag ccacaagccc gagccaagcc cgtgccagcg tgcgaaggcc
     5401 cggcgcgaag cgcaactgga ccagcttggg ctcaagcgca cgttcgatga cctgagccgg
     5461 atcgatgcgg cggtgcgggt ggtgtgcaac gggttctagg tagcggtcat ggctgtcttg
     5521 acagacataa taagcattat gcgaagttat aagctgccgc gccggcggtc aatacaccgg
     5581 acgcaggacc ccggccaggc gcgccccggc ccagaccagc atgaagccgc cgaccagggt
     5641 cgcgccgagg tagccaagca acaggtcgct gcgttgcgag cgcgccatca gcaggcattc
     5701 cagcatcagc gccgagtacg tggtcagcgc gcccagcacg ccgacgatca ggaacgcacg
     5761 ccagtacagc gcatgcggtc cacggccctc cagccacacc gcgaggaagc cgaccgcgaa
     5821 ggaaccgacc aggttggcgg caagcgtccc gtacgggatg ccggtgccga actggcgcag
     5881 catcgcgccg cccagccagt agcgtgcgcc ggcaccaagc gcgccgccca gcatcaccag
     5941 cgcaagctgt tgccaccaca ggttcatggg cggttcttct gcttgcgcac ctgcgtgcgc
     6001 gccgcacgca gcgtgtccag cttttccttg agcttgcctt ccatgccgcg ttgcaccggc
     6061 tggtagtaca cccgctcgcc catcgcgtcg gggaagccgg tctggtccag cgcgatgccg
     6121 ccatcgacgt cgtggtcgta ctggtagtcc ttgccgaagc caagcgattt catcagccgg
     6181 gtcggcgcgt tgcgcaggtg cagcggcacg tcctgggtgc cgtgcgcttc gacatccgca
     6241 cgcgcggcat tgaaggcgcg ataggccgcg ttcgatttcg cggtgctggc caggtacagg
     6301 gtgagctgcg caagcgcgag ttcgccttcc ggcgaaccca ggcggtcata ggcgttccag
     6361 gcttcgatcg ccatctgcag cgcgcgcggg tctgcaaggc cgatgtcctc aaccgccatc
     6421 cgggtcaacc gccgcgcgag gtagccggga tccgcgccgc cgtccagcat tcgcgccagc
     6481 cagtacagcg ccgcgtcagg attggagctg cgcacgcatt tgtgcagtgc cgagatctgg
     6541 tcgtaggact ggtcgccgcc cttgtcgaag cggcgggtgc ggtcggcgag cacctggtgc
     6601 agggtcgcct cgctgatgca gccaccctcg ccgtccgcaa ggtcggcggc gatttccagc
     6661 agcgtgagtg cgcggcgcac gtcgccgtcg gcggcgccgg cgatctcgcg cagtgcggca
     6721 tcggcgacct cgaggcagcg accgccgagg ccgtgttctg catcgtccag cgcatgccgc
     6781 agcgccaacg cgatgtcgtc cgcggacacc gcctccagca cgtgcacgcg gcaacgcgac
     6841 agcagcgcgg aattgagttc gaacgaggga ttctcggtgg tggcgccgac gaacaggatg
     6901 gtgccgcgct cgatgtgcgg caggaatgcg tcctgctgcg ccttgttgaa gcggtgcacc
     6961 tcgtccacga acagcaccgt gcggcggccg gcctggaatc gcgcctgcgc ctgcgccagc
     7021 acctcgcgca cctgcggcag gcctgacagc accgccgaga tcgccacgaa ttcggcatcg
     7081 gcgtggcgcg caagcaacag cgccagcgtg gtcttgccgc agccgggcgg gccccacagg
     7141 atcatcgaat ggacatgccc gccctccacc gcccgccgca gcgcggaccc gggcgcgagc
     7201 agccggcgct ggccaaccat gtccgccagc gcgcgcgggc gcatgcgttc ggccagcggg
     7261 cgcagcgagg cgctggcggc gtcttgcagt tcgggcaggt ctgcttgctt gggtggtgcg
     7321 ggcatcccgc gattctacgc gggcatcgtc aag
//
LOCUS       DAORUK010000105         7240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_11708_length_7240_cov_18.957968, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000105 DAORUK010000000
VERSION     DAORUK010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7240
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11708_length_7240_cov_18.957968"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..889
                     /locus_tag="PK818_07730"
     CDS             185..889
                     /locus_tag="PK818_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HQY82654.1"
                     /translation="MIEFGHLSHVGLRRELNEDTYYGDSELGLWLVADGMGGHEYGEV
                     ASALAREAIVREIRGGVPLAEAIRIADAEIIRCSRRRGDSLPMGTTVVAAKVSGNRFE
                     VAWVGDSRVYLWRDGALTQISQDHSYVQELIAQGAITADQARSHPHRNVVTQALGVTD
                     PAQLNVETLTGELRPGMQLLLCSDGLTEEVDDRSIARVLAHQECSAQECVDGLVAAAL
                     DGGGSDNVTVVLVRRH"
     gene            complement(893..1615)
                     /gene="dnaQ"
                     /locus_tag="PK818_07735"
     CDS             complement(893..1615)
                     /gene="dnaQ"
                     /locus_tag="PK818_07735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017914812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HQY82655.1"
                     /translation="MAADLKRQVVLDTETTGLEWKKGNRVVEIGCVELVERRPTGRTF
                     HCYLNPDREFEPGAQQVTGLTLEFLQDKPRFAAVVDEFLAFVEGAELVIHNAAFDLGF
                     LDAELARLDGYPGMSGRCTVEDSLLLARQRFPGQRNSLDALCKRLGVDNSQRALHGAL
                     LDAQILTDVYLALTAGQGEIGFASEAPKAAVVLAPTPVATADARPRVLLQADEAEAHA
                     ARLQVLQKKAGRCLWLELEAAD"
     gene            complement(1615..2073)
                     /gene="rnhA"
                     /locus_tag="PK818_07740"
     CDS             complement(1615..2073)
                     /gene="rnhA"
                     /locus_tag="PK818_07740"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HQY82656.1"
                     /translation="MSVARKRVDIHTDGACLGNPGPGGWGALLRWRGQERELAGGEAD
                     TTNNRMELMAAICALEALKEDCDAILHTDSQYVRKGITEWMPNWVRRGWKTAGGDPVK
                     NRDLWERLHAAAARHAVDWRWVKGHAGDPDNERVDQLARAAAVRIRERER"
     gene            complement(2070..2744)
                     /locus_tag="PK818_07745"
     CDS             complement(2070..2744)
                     /locus_tag="PK818_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQY82657.1"
                     /translation="MPPTLPPSAFRRQPETAAAAAWFAGVAGQAILASEADSLRQAAQ
                     ERPGQAGLWLCPSGMGEGEVDLPLLRLRAAARGFEGDVRCGLPLPLGSESCAVVVVQH
                     LADISSDPAALLDECARVLVAGGRLWLLALNPLSPYRLRWRGQGPRVAEPVTWRRRLR
                     AVGLVPDAVSLGLGPTWKIAPEPALQDGAGVRAAFLLRAEKRRSPLTPMRAPSAARWE
                     PGIPAA"
     gene            2796..3560
                     /gene="gloB"
                     /locus_tag="PK818_07750"
     CDS             2796..3560
                     /gene="gloB"
                     /locus_tag="PK818_07750"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HQY82658.1"
                     /translation="MDLQPLPAFDDNYIWTLRDAGGRALLVDPGDAAPVLAALGDGPR
                     PHAILLTHHHGDHVGGVPGLLRRWPGLAVIAPHDARIACASQRVGDGEALEVGPWRFH
                     VLATPGHTSSHACFFGEGVLFCGDTLFSLGCGRLFEGTPAQMHASLHRLAGLPGDTVV
                     CCGHEYTRANAAFALAVDPGNAALHDRANEVAALRLAGQPTLPTTLACERACNPFLRV
                     DDAAVRAAIEAHAGRRIDGDIDAFAALRRWKDGFRA"
     gene            3557..4213
                     /locus_tag="PK818_07755"
     CDS             3557..4213
                     /locus_tag="PK818_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQY82659.1"
                     /translation="MNAARLGWLAALLVAAMPLRSSPAGPPAATIESISARFWSDVAE
                     PECGNAPRRWLVHYADVPRRLRNPREARLRADVARVLDALDAAGLPSEFALIPFVESR
                     YRATARSPGGQLGLWQFTAATARRNGLEVRPGHDQRLDPEASTRAAVRYLRRLHDLFG
                     ADWRWTVMAYNAGEGAARRARRNGGARKLSPIARHYPDKLHALACLLPRPPDQGRPAG
                     "
     gene            4256..5044
                     /locus_tag="PK818_07760"
     CDS             4256..5044
                     /locus_tag="PK818_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HQY82660.1"
                     /translation="MGFLQGKRALVTGIASQRSIATGIADAMHRQGAELALTYQNEKL
                     KTRVEAAAAEYGSNIVLPLDVADDAQIDACFDELGKHWGDGFDILVHCIAFAPREAIE
                     GDFLDGMSRDNFRIAHDISAYSLAALGKAARPLMKGRNGSILTLSYLGAERALANYNT
                     MGVAKASLEATVRYMAMALGPEGTRVNAISAGPIRTLAASGVANFRKMLTQFEAVAPL
                     RRVVTIEDVGNAAAFLCSDLAAGITGEITYVDAGYNIMGMSGIE"
     gene            complement(5115..7082)
                     /locus_tag="PK818_07765"
     CDS             complement(5115..7082)
                     /locus_tag="PK818_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HQY82661.1"
                     /translation="MLQKLRDKTSGWIATVILGLLIIPFAFFGMESYLSQRVETHAAR
                     IAQPPSWWPSAPDAWPFSTLWTRHDIESSEFRKRFEVARMRARDEQGDKFDARAFESV
                     ENKRKILDDMIDEQVMRLAAERDGIVVSDSEVRAAIQEVPDFQVDGKFNADRYQMLLG
                     SQSPPLTPREFEQNIRESLQYGLIPSRLAQSAFATDGEVERVLRLLGEQRDVSFVAMP
                     QVPADTAPVTAAQIEAWYKANAASYRRAETVRLEYVEVDGSKLPAPVIDEALLRKRYQ
                     EQGAKYATAEQRSVSHILVQVAADASDADRKAAEARANKLAGQARAPGADFAALAKAG
                     SDDVGSRDKGGDLGLMVKGGLPGPFEDAAFSMQAGEVRGPVKSDFGWHVIKVNEIRAG
                     NQQPFEAVREQLQAELQETEHERAYNELSGKLVDAVYKNPNSLEPAARALGLPVQATA
                     AFSRAGGTGIAANQNVLRAAFSETLLQDGTASDPIELGPNHSVMIRVLEHEPERALPL
                     ASVADSVVAAIRNDRQRKAAEAAADALVKAARAKGLAAAAAGATLVMAEVNGLQRRSQ
                     SPSPRAVEAFFNTPRPQDNLIPVDKALIGGQYLVYAIRAVRDGDVTKATAEERKQLRE
                     QLSAVAGGAAQKAHVKAARAKYGITVAEDRL"
BASE COUNT         1047 a         2686 c         2424 g         1083 t
ORIGIN      
        1 accgggggtt cgaatccccc cctctccgcc agacatgaac aacgccccga tggggcgttt
       61 ttcatgcctg cccgacgact ttcgccccca cctccaccgc cgcaaccgtt acattcccgg
      121 cacgcgtccg gttcgcccgc acgtttccgt tagccccctc aagccccgga tgaatgtcgc
      181 ccgcatgatc gaattcggac atctcagcca tgtcggcctg cgccgggaac tgaacgagga
      241 cacctattac ggcgacagcg aactcggcct gtggctggtc gcggacggca tgggcggcca
      301 cgagtacggc gaggtcgcca gcgcgctggc gcgcgaggcc atcgtccgcg agatccgtgg
      361 cggcgtaccg ctggcggagg cgatccggat cgccgatgcg gagatcatcc gctgctccag
      421 gcgccgcggc gacagcctgc cgatgggcac caccgtggtc gcggccaagg tcagcggcaa
      481 tcgcttcgag gtggcgtggg tcggcgacag ccgcgtctac ctttggcgcg atggcgcgct
      541 gacccagatc tcgcaggacc acagctacgt gcaggaactg atcgcgcagg gcgcgatcac
      601 cgccgaccag gcgcgcagcc acccgcaccg caatgtcgtc acccaggcgc tgggcgtcac
      661 cgacccggcc cagctcaatg tcgagaccct gaccggcgaa ttgcgcccgg gcatgcagct
      721 gctgctgtgc agtgatggcc tgaccgagga agtggacgac cgcagcatcg cgcgggtgct
      781 ggcgcaccag gaatgcagcg cgcaggaatg cgtggatggc ctggtcgcgg ccgcgctgga
      841 tggcggcggc tccgacaacg tcaccgtggt gctggtgcgc cgccactgag cgtcaatcgg
      901 cggcttcgag ctccagccac aggcatcgcc cggctttctt ctgcaggacc tgcaggcggg
      961 ccgcatgcgc ttcggcttcg tcggcctgca acagcacccg tggccgtgca tcggcggtcg
     1021 ccaccggcgt cggcgccagc acgaccgccg ccttcggcgc ctcgctggcg aagccgatct
     1081 cgccctgccc cgcggtcagc gccaggtaga cgtcggtgag gatctgcgca tccagcagcg
     1141 ccccgtgcaa ggcgcgctgc gaattgtcga cgccgagccg cttgcacaag gcgtccagcg
     1201 aattgcgctg gccggggaag cgttgccgcg ccagcagcag ggaatcctcg acggtgcagc
     1261 gcccggacat gccggggtag ccgtccagcc gcgccagttc cgcatccagg aagccgaggt
     1321 cgaaggccgc gttgtggatg accagttccg cgccttcgac gaacgccagg aattcatcca
     1381 ccaccgcggc gaaacgtggc ttgtcctgca ggaactccag ggtcaggccg gtgacctgct
     1441 gcgcgcccgg ctcgaattcg cggtccgggt tgaggtagca atggaaggtg cggccggtcg
     1501 ggcgacgctc gacgagttca acgcagccga tctcgaccac gcggttgccc ttcttccatt
     1561 ccaggccggt ggtctcggtg tcgagcacca cttgcctctt caaatccgcg gccatcagcg
     1621 ttcccgttcc ctgatcctga ccgccgccgc gcgcgccagc tggtcgacgc gttcgttgtc
     1681 cgggtcgccg gcgtggccct tgacccagcg ccagtccacc gcgtgccggg ccgccgccgc
     1741 gtgcaggcgt tcccacaggt cgcggttctt gaccgggtcg ccgccggcgg tcttccaccc
     1801 gcggcggacc cagttcggca tccattcggt gatgcccttg cggacgtact gcgagtcggt
     1861 gtgcaggatc gcgtcgcagt cctccttcag cgcttccagc gcacagatcg ccgccatcag
     1921 ctccatccgg ttattggtgg tgtcggcctc gccaccggcg agttcgcgct cctggccgcg
     1981 ccagcgcaac agggcacccc agccgccggg cccgggattg cccaggcacg cgccatcggt
     2041 gtggatgtcg acccgcttgc gcgccacgct catgccgccg ggatccccgg ttcccagcgc
     2101 gccgccgacg gcgcccgcat cggggtcagc ggcgagcgcc gcttctccgc gcgcagcagg
     2161 aacgccgcgc gcacgccggc gccgtcctgc agcgccggtt cgggggcgat cttccaggtc
     2221 ggccccagcc ccagcgacac cgcgtccggc accagcccga ccgcacgcag gcggcgccgc
     2281 caggtcaccg gttcggccac ccgcggccct tgcccgcgcc agcgcaggcg atacggcgac
     2341 aacgggttca gcgccagcaa ccacaggcgg ccgcccgcga ccagcacgcg cgcgcattcg
     2401 tcgagcagcg ccgcgggatc cgaactgatg tcggccaggt gttgcacgac caccacggcg
     2461 cacgactcgc tgcccagcgg cagcggcagc ccgcagcgca cgtcaccctc gaagccgcgt
     2521 gccgccgcgc gcaggcgcaa cagcggcagg tcgacctcgc cttcgcccat cccgctgggg
     2581 caaagccaca ggcccgcctg gccggggcgt tcctgcgccg cctggcgcag gctgtccgcc
     2641 tcgctggcca ggatcgcctg cccggccacg cccgcgaacc acgccgccgc ggcggcggtt
     2701 tcgggttgac ggcggaaagc ggacgggggc aaggtcggcg gcatgccccc cggattctgc
     2761 gcgaaaggcg gcgacccgtc acgactggac aacgcatgga tttgcagcca ctgcccgcgt
     2821 tcgacgacaa ctacatctgg acgctgcgcg atgccggggg ccgcgcgctg ctggtggatc
     2881 cgggcgacgc ggcaccggtg ctggcggcgc tgggcgacgg cccgcgcccg cacgcgatcc
     2941 tgctgaccca ccaccacggc gaccacgtcg gcggagtgcc cggactgctg cggcgctggc
     3001 ccggccttgc ggtcatcgcc ccgcacgacg cgcgcatcgc ctgcgccagc cagcgggtcg
     3061 gcgatggcga ggcactcgaa gtgggcccgt ggcgtttcca cgtcctcgcc acgcccggcc
     3121 acaccagcag ccatgcctgc ttcttcggcg aaggcgtgct gttctgcggc gacaccctgt
     3181 tctcgctggg ctgcgggcgc ctgttcgaag ggacgccggc gcagatgcac gcctcgttgc
     3241 acaggctggc cggcttgccc ggcgacaccg tggtttgctg cggccacgag tacacgcgcg
     3301 ccaacgccgc gttcgcactg gcggtggatc ccggcaacgc cgccttgcac gatcgtgcca
     3361 acgaggtcgc ggcactgcga ctggccgggc agccgaccct gccaaccacg ctcgcgtgcg
     3421 aacgcgcctg caacccgttc ctgcgggtcg acgacgccgc ggtccgcgcc gcgatcgagg
     3481 cacatgccgg tcgccgcatc gacggcgaca tcgacgcctt cgccgcactg cggcgctgga
     3541 aggacgggtt ccgcgcatga acgcggcccg gcttggctgg cttgccgccc tgctggtcgc
     3601 ggccatgccg ctgcggtcga gccccgcagg cccgcccgcc gcaaccatcg agtcgatctc
     3661 cgcacggttc tggagcgacg tcgccgagcc cgaatgcggc aacgcgccgc gtcgctggct
     3721 ggtccactac gccgatgtcc cgcgccgcct gcgcaacccg cgcgaggcgc gcctgcgcgc
     3781 ggacgtcgcg cgcgtgctcg atgcactcga tgccgccggc ctgcccagcg aattcgcgct
     3841 gatcccgttc gtcgagagcc gctaccgcgc caccgcgcgc agccccgggg gccagctggg
     3901 cctgtggcag ttcaccgccg ccaccgcgcg ccgcaacggg ctggaggtgc gccccggcca
     3961 cgaccagcgg ctggacccgg aggcctccac ccgcgccgcg gtccgctacc tgcgccgcct
     4021 gcacgacctg ttcggtgccg actggcgctg gacggtgatg gcctacaacg ccggcgaagg
     4081 cgccgcacgc cgcgccaggc gcaacggcgg cgcgcgcaag ctgtcgccga tcgcccgcca
     4141 ctatccggac aaactgcatg cgctggcctg cctgctgccg cgcccgcctg accagggccg
     4201 ccccgcgggg taaactggag cctttgctct acccgcatcc gcaccggaga acaccatggg
     4261 tttcctgcaa ggcaagcgcg cactggtcac cggcatcgcc agccagcgtt cgatcgccac
     4321 cggcatcgcc gatgccatgc accgccaggg cgccgaactg gcgctgacct accagaacga
     4381 aaagctcaag acgcgggtgg aagccgccgc cgccgaatac ggcagcaaca tcgtcctgcc
     4441 gctggacgtg gccgatgacg cccagatcga cgcctgcttc gacgaactcg gcaagcactg
     4501 gggcgacggc ttcgacatcc tcgtgcactg catcgcattc gccccgcgcg aggcgatcga
     4561 gggcgacttc ctcgacggca tgagccgcga caacttccgc atcgcccacg acatcagcgc
     4621 gtactcgctg gccgcgctgg gcaaggccgc gcgtccgctg atgaaggggc gcaacggttc
     4681 gatcctgacc ctgagctacc tcggcgcgga gcgagcgctc gccaactaca acacgatggg
     4741 cgtggccaag gcctcgctgg aagccaccgt gcgctacatg gcgatggcgc tggggccgga
     4801 gggcacgcgc gtcaatgcga tctcggcggg cccgatccgc accctggccg ccagtggcgt
     4861 cgccaacttc cgcaagatgc tcacccagtt cgaggccgtg gcgccgctac gccgggtggt
     4921 caccatcgag gacgtcggca atgccgccgc gttcctgtgc tcggacctgg ccgccggcat
     4981 caccggcgag atcacctacg tcgacgccgg ctacaacatc atgggcatga gcgggatcga
     5041 gtgatccgcg gccaaccctg gaaacaggaa cgcccggcaa tgccgggcgt tccttttggt
     5101 gcatggcgcc gaagtcaaag ccggtcttcg gccaccgtga tcccgtattt cgcgcgcgcg
     5161 gccttcacgt gggccttctg cgccgcgccg ccggcgaccg ccgacagctg ctcgcgcaac
     5221 tgcttgcgct cttccgccgt cgccttggtg acgtcgccgt cgcgcaccgc gcgaatcgcg
     5281 tacacgaggt actggccgcc gatcagggcc ttgtcgaccg ggatcaggtt gtcctgcggg
     5341 cgcggggtgt tgaagaaggc ctccaccgcg cgcggcgaag gcgactggct acggcgctgc
     5401 aggccgttga cctccgccat caccagcgtc gcgcccgcgg ccgcggcggc caggcccttg
     5461 gcgcgggctg ccttgaccaa cgcgtcggcc gcggcttcgg cggccttgcg ctggcggtcg
     5521 ttgcggatcg cggcgaccac cgagtccgcg accgacgcca gcggcagggc acgttccggc
     5581 tcgtgctcca gcacccggat catgacgctg tggttggggc caagttcgat cggatcgctg
     5641 gcggtaccgt cctgcagcaa cgtttcggag aacgccgcac gcagcacgtt ctggttggcg
     5701 gcgatgccgg tcccaccggc acgactgaac gccgcggtgg cctgcaccgg cagtccgagc
     5761 gcgcgcgccg ccggctccag cgagttcggg ttcttgtaca cggcatcgac cagcttgccg
     5821 ctcagttcgt tgtaggcacg ctcgtgctcg gtttcctgca gctccgcctg cagttgctcg
     5881 cggaccgcct cgaacggctg ctggttgcct gcgcggatct cgttgacctt gatcacgtgc
     5941 cagccgaaat cgctcttgac cggcccgcgc acttcgccag cctgcattga gaacgcggca
     6001 tcctcgaacg gtcccggcag gccacccttg accatcaggc cgaggtcgcc acccttgtcc
     6061 ctggagccaa cgtcgtccga accggccttc gccagcgcgg cgaagtccgc gcccggcgcc
     6121 cgcgcctgcc ctgccagctt gttcgcgcgc gcttccgcgg ccttcctgtc ggcatcgctg
     6181 gcgtccgccg cgacctgcac caggatgtgc gagaccgacc gctgttcggc agtggcgtac
     6241 ttggcgccct gctcctggta gcgcttgcgc agcaacgcct cgtcgatcac cggcgccggc
     6301 aacttgctgc catccacctc gacgtactcc aggcgcacgg tctcggcacg gcgatagctg
     6361 gcggcgttcg ccttgtacca ggcttcgatc tgcgcggcgg tcaccggcgc ggtatccgcc
     6421 gggacctgcg gcatcgccac gaagctgacg tcgcgctgtt caccgagcag gcgcagcacg
     6481 cgctcgacct cgccatcggt ggcgaacgcg gattgcgcca gccggctggg gatcagcccg
     6541 tattgcaggc tctcgcggat gttctgctcg aactcgcgcg gcgtcagcgg cgggctctgc
     6601 gagcccagca gcatctggta gcggtcggcg ttgaacttgc cgtcgacctg gaaatccggg
     6661 acctcctgga tcgccgcacg cacctcgctg tcgctgacca cgatgccgtc gcgctcggcg
     6721 gccaggcgca tgacctgctc gtcgatcatg tcgtcgagga tcttgcgctt gttctcgacc
     6781 gactcgaacg ccctggcgtc gaacttgtcg ccctgctcgt cgcgcgcgcg catgcgcgcg
     6841 acctcgaagc gcttgcggaa ctcgctgctc tcgatgtcgt gccgcgtcca cagcgtgctg
     6901 aacggccatg cgtccggcgc cgacggccac cacgacggcg gctgggcgat gcgcgcggca
     6961 tgggtctcca cccgctgcga caggtacgac tccatgccga agaaggcgaa cgggatgatc
     7021 agcaggccga ggatcacggt ggcgatccag ccggaggtct tgtcgcggag tttctgcagc
     7081 atgggcatcg gcgatgaggc gtagccgctt agtttagcgc gcgatccggc ccggtacgca
     7141 tcgcgggtgc cgcgggcacg ccaggcgatg caaaaaagaa gccccgcatc gctgcagggc
     7201 ttcggtacga cgtaacgcta aaaagatggc ggagtggacg
//
LOCUS       DAORUK010000106         7185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_11841_length_7185_cov_21.302525, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000106 DAORUK010000000
VERSION     DAORUK010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11841_length_7185_cov_21.302525"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="PK818_07770"
     CDS             <1..932
                     /locus_tag="PK818_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQY82662.1"
                     /translation="PDHDWTLLVQDMDKWDADIRALLAGFAFLPRWRIDDVMVSFAAT
                     GGSVGAHIDQYDVFLLQAQGRRHWQIDASANPPLGFRPDVELKLLREFHPTHDWVLEP
                     GDVLYLPPGVPHHGIAVDHCLTFSVGMRAPSAAELLGDFVDTLAADAPEDLRYTDPGL
                     APASDANEIDGAAMARVVEALNLLRMNDADALGDWFGAFITTYRCAVEPAADDAGRAR
                     IEVEWDLAHGAVILRHPWSRMAWRRAHGKRGGRLYAGGRVLALPVRDARRLAAAEAVD
                     AALYDSLGQAGRDAVFALLDAGHYRLQLAEDAQ"
     gene            929..1852
                     /locus_tag="PK818_07775"
     CDS             929..1852
                     /locus_tag="PK818_07775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY82663.1"
                     /translation="MNPNDPGLQPFHVSVVDFQAALAELRAVRDEVFVGEQGVPVEIE
                     HDALDPACTHVIARLLDGTTIGTARLTPDQHIGRMAVRAPWRGRGVGDALLLALVAEA
                     RKRGWTEVRLNSQVSALGFYERHGFKPEGDRFMEAGIEHQAMLRMLDGPQRIESRAAG
                     VEATLNAIDSARRSIWIRSRDLDPLLYTDSGVLQALRRFATAGRGGEVRILLHDVATP
                     QRSQAPLLALAQRLPSIFQFRELQDPVDRDDPAAFIAGDGGGYYLRSMGERIDGEAEP
                     HAPARVRQLRAAFDEAWERSRPVSEYRALGL"
     gene            2031..4859
                     /locus_tag="PK818_07780"
     CDS             2031..4859
                     /locus_tag="PK818_07780"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HQY82664.1"
                     /translation="MVDSLLKQFAQSSQLGASGAYIEDLYEQYLVAPESVGPKWKQYF
                     DGLKGREAGDVPHSVVIEQIAEAGRRAARGVVASGGGDERERAVGKLITAYRSRGHLA
                     ANLDPLGMAVKPDAPDLALAFHRLSEGDAGAEFSTGGVAGRERMKLGELLATLKATYT
                     GSIGAEFMHITDVEQRRWVYERLEAAAGNYGRTPADKQRILERLTAAEGLERYLHTKY
                     VGQKRFSLEGGDSLIPLMDTTIRRAGAQGAKDVVIGMAHRGRLNVLVNTLGKPAQHLF
                     DEFEGKFEHVHSDLAHQGDVKYHMGFSADVATPGGPVHLALAFNPSHLEIVNPVVAGS
                     VRSRQTRRGGHGRAQVLPILIHGDAAFAGQGVGMELLQMSQARGFAVGGTVHVVVNNQ
                     VGFTTSAVQDARSTLYCTDVAKMVGAPVLHVNGDDPEAVVFCAELALDFRNRFGKDVV
                     IDLVCYRRHGHNEADEPAATQPLMYRTIRAMKTTRELYANRLAAEGTIAADAAQALVD
                     AYRDRLDAGEVTTEVVARTPDEFAVDWSPFMAGKLADAVDTRFDGTRLKSLADAINHI
                     PDDLKLHPRVAKIYEDRRKMAAGELPGDWGFAENLAYASLLDEGYRLRLVGQDCGRGT
                     FFHRHAILHEQTSDEYVLPLRRLVSNPSDVAIIDSLLSEEAVMAFEYGYATADPMTLD
                     IWEAQFGDFANGAQVVIDQFLAAGEAKWGRLCSLALFLPHGYEGQGPEHSSARLERFL
                     QLCALDNMIVCAPTTPAQDFHMIRRQMRMRTRKPLVVMTPKSLLRHKLAVSSLDELAN
                     GEFQRLIPDTTATAKAVRRVVACSGKVYYDLLEEAQRNGIDDVALVRVEQLYPFPRQE
                     LAAELQRFAAATEVVWCQEEPQNQGAWYQIQHHLRACLQPGQSLSYAGRARSPSPAAG
                     HMAEHVEEHSALVADALVNPIAGEICPE"
     gene            4906..6084
                     /gene="sucB"
                     /locus_tag="PK818_07785"
     CDS             4906..6084
                     /gene="sucB"
                     /locus_tag="PK818_07785"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008572000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HQY82665.1"
                     /translation="MATEVKVPVLPESVSDATIASWHKKAGDAVRRDENLLDLETDKV
                     VLEVPSPVDGVLKEIRFEAGATVNSQQVVAVIEEGAVAAAPAAAAAAPAAAPAAAAPA
                     APASAPASGALPPGARFAATTSGVDPADVAGTGRRGMVTKEDIVNYASGKTAGVAGGA
                     RPEERVPMTRMRARIAERLMQSKNSIAMLTSFNEVNLAKVMQMRKELGEQFEKANGIK
                     LGFMGFFAKAAANALQKYPVVNASVDGNDVIYHGYADISVAVSTDKGLVTPVLRNVER
                     MSFADVESGIATYAKAAREGGLKLEDLQGGTFTITNGGTFGSLMSTPIVNPPQSAILG
                     MHAIKERAIVENGQVIAAPMMYIALSYDHRIIDGKDAVLFLVDIKNQLENPHRMLLGM
                     "
     gene            6141..>7185
                     /locus_tag="PK818_07790"
     CDS             6141..>7185
                     /locus_tag="PK818_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQY82666.1"
                     /translation="MSEVPASQQQAFDVVVIGAGPAGYHAAIRAAQLGLKTACIDAAL
                     GKDGKPALGGTCLRVGCIPSKALLDSSRQFWNMGHLFDQHGISFETPAIDVKKMVARK
                     DGIVKQFTGGIAALFKANKITAFHGFGQLQPGNVVSVKQHDGSTVELKGTNVILAAGS
                     DSIELPFARFGEHIIDNVGALDLEKVPKRLGVIGAGVIGLELGSVWKRLGAEVTILEG
                     LPTFLAAADAEVGKVAAREFKKQGLDIKLGCKCTATEVKKDGVHISYTDDKGAAQELV
                     VDKLLVAVGRRAATKGLLAEGTGVKLNERGQIEVDAHCHTGVDGVWAVGDCVRGPMLA
                     HKGFEEGIAVAELI"
BASE COUNT         1199 a         2513 c         2436 g         1037 t
ORIGIN      
        1 tgcccgacca cgactggacc ctgctggtgc aggacatgga caagtgggac gccgatatcc
       61 gcgccctgct tgccggcttt gcgttcctgc cgcgctggcg gatcgacgac gtgatggtga
      121 gcttcgccgc caccggcggt tcggttggcg cgcacatcga ccagtacgac gtgttcctgc
      181 tgcaggcgca gggccgccgc cactggcaga tcgacgccag cgcgaacccg ccgctgggtt
      241 tccgccccga tgtcgaactc aagctgctgc gcgaattcca tcccacccac gactgggtgc
      301 tggaacccgg cgacgtgctc tacctgccgc ccggcgtgcc gcaccatggc atcgcggtcg
      361 atcattgcct gacgttctcg gtcggcatgc gcgcgccctc ggcggcggaa ctgctcggcg
      421 atttcgtcga cacccttgcc gccgatgcgc ccgaggacct gcgctacacc gatcccggcc
      481 ttgcgccggc cagcgacgcc aacgagatcg acggcgcggc gatggcgcgg gtggtggagg
      541 cgctcaacct gctgcggatg aacgatgccg acgcccttgg cgactggttc ggtgccttca
      601 tcaccaccta ccgctgcgcg gtggaaccgg ccgcggatga cgccgggcgc gcgcggatcg
      661 aggtcgaatg ggaccttgcg cacggtgccg tgatcctgcg gcatccgtgg tcgcggatgg
      721 cgtggcggcg cgcacacggc aagcgcggcg gccgcctgta tgccggtggc cgcgtgctcg
      781 cgctgccggt gcgcgacgcc cggcggctgg ccgccgccga ggcggtggat gccgcgctgt
      841 acgacagcct gggccaggcc ggccgcgacg ccgtgttcgc gctgctcgat gccggccatt
      901 accgtttgca actggcggag gacgcgcaat gaaccccaat gatccaggcc tgcaaccgtt
      961 ccatgtcagc gtcgtcgatt tccaggcggc gctggccgaa ctgcgcgcag tgcgcgacga
     1021 ggtcttcgtc ggcgagcagg gcgtcccggt cgagatcgag cacgatgcgc tggatccggc
     1081 atgcacgcac gtgatcgcgc ggctgctgga cggcaccacc atcggcaccg cgcgtttgac
     1141 cccggaccag cacatcgggc ggatggcggt gcgcgcgccg tggcgcggcc gcggggtcgg
     1201 cgatgcgctg ctgctggcac tggtcgcgga ggcccgcaaa cgcggctgga ccgaagtccg
     1261 gctgaattcg caggtgtcgg cgctcgggtt ctacgagcgc catgggttca agcccgaggg
     1321 tgaccgcttc atggaagccg ggatcgagca ccaggcgatg ctgcggatgc tggatggtcc
     1381 gcaacgcatc gagtcgcgcg ccgccggggt cgaggcgacc ctgaacgcca tcgacagcgc
     1441 ccgccgcagc atctggatcc gcagccgcga tcttgaccca ttgctgtata cggattcagg
     1501 cgtgctgcag gccctgcgcc ggttcgccac cgccggccgc ggcggcgagg tgcggatcct
     1561 gctgcacgac gtcgccaccc cgcaacgttc gcaagccccc ctgctggcgc tggcgcaacg
     1621 gctgccgagc atcttccagt tccgcgagct gcaggacccg gtggaccgcg acgaccctgc
     1681 cgcattcatc gcgggcgatg gcggcggcta ctacctgcgc agcatgggcg agcggatcga
     1741 cggcgaggcc gaaccgcatg ccccggcacg cgtgcgccag ctgcgcgcgg ccttcgacga
     1801 ggcctgggag cgttcgcggc cggtcagcga ataccgcgca ctcgggctgt gaagcacggc
     1861 cggtgccgct agcggccagc ggctggaggc cgtcttagac aaaagacgtg gtgcagtgcg
     1921 caaccgcctg cggctataat tcccgtcctt tcgcccgccc attcagcggg caccggctgg
     1981 acatcgctcc tcccggatca atcacttatc tccaggtcgc cctcgacgcc atggtggaca
     2041 gtctcctgaa gcagtttgcc cagtcctcgc aacttggcgc cagcggcgcc tacatcgagg
     2101 acctctacga gcagtacctg gtcgcgccgg aaagcgtcgg gccgaagtgg aagcagtact
     2161 tcgacgggct caagggccgc gaagccggcg acgtcccgca ttcggtggtg atcgaacaga
     2221 tcgccgaggc cggccgccgg gccgcgcgcg gggtcgtcgc cagtggcggc ggtgacgagc
     2281 gcgaacgcgc ggtcggcaag ctgatcaccg cctaccgttc gcgcgggcac ctggccgcca
     2341 acctggatcc gctgggaatg gcggtcaagc cggatgcacc cgacctggcg ctggcattcc
     2401 accgcctgtc cgagggcgat gccggcgccg aattcagcac cggcggcgtg gctggccgcg
     2461 agcgcatgaa gctcggcgag ctgctcgcca ccctgaaggc gacctacacc ggcagcatcg
     2521 gcgccgagtt catgcacatc accgacgtcg agcagcggcg ctgggtgtat gaacgccttg
     2581 aggccgcggc cggcaattac gggcgcacgc ctgcggacaa gcagcggatc ctggagcggc
     2641 tgaccgccgc ggaaggcctg gagcgctacc tgcacaccaa gtacgtgggg cagaagcgct
     2701 tctcgctgga aggcggtgat tcgctgatcc cgctgatgga caccaccatc cgccgtgccg
     2761 gcgcgcaggg cgcgaaggac gtggtcatcg gcatggccca ccgtggccgc ctgaacgtgc
     2821 tggtgaacac cctcggcaag ccggcgcagc acctgttcga cgaattcgaa ggcaagttcg
     2881 agcacgtcca tagcgacctg gcccaccagg gcgacgtcaa gtaccacatg ggtttctcgg
     2941 ccgacgtcgc caccccgggc ggcccggtcc acctggcgct ggcgttcaac ccgtcgcacc
     3001 tggagatcgt caacccggtg gtcgccggct cggtgcgttc gcgccagacc cggcgcggcg
     3061 ggcacggccg tgcgcaggtg ctgccgatcc tgatccacgg tgacgctgcg ttcgccggcc
     3121 agggcgtggg catggagctg ctgcagatgt cgcaggcccg cggcttcgca gtcggcggca
     3181 ccgtccacgt ggtggtcaac aaccaggtcg gcttcaccac cagcgcggtg caggacgcgc
     3241 gctccaccct gtactgcacc gacgtcgcca agatggtcgg cgcgccggtg ctgcacgtga
     3301 atggcgacga cccggaagcg gtggtgttct gcgccgaact ggcgctggac ttccgcaacc
     3361 gcttcggcaa ggacgtggtc atcgacctcg tctgctaccg ccgccatggc cacaacgagg
     3421 ccgacgagcc ggcggcgacg cagccgctga tgtaccgcac gatccgcgcg atgaagacca
     3481 cccgcgagct gtatgcgaac cggctggccg ccgaaggcac gatcgccgcg gacgcggcgc
     3541 aggcgctggt ggacgcctac cgcgaccggc tcgacgccgg cgaggtcacc accgaggtgg
     3601 tcgcccgcac ccccgacgaa ttcgcggtcg actggtcgcc gttcatggcc ggcaagctcg
     3661 ccgatgcggt cgatacccgc ttcgacggca cccgcctgaa gtcgctggcg gatgcgatca
     3721 accacatccc ggacgacctc aagctgcacc cgcgggtcgc caagatctac gaggaccgcc
     3781 gcaagatggc ggccggcgaa ttgccgggcg actggggctt cgccgagaac ctggcctacg
     3841 ccagcctgct cgacgaaggc taccgcctgc gcctggtcgg gcaggactgc ggccgcggca
     3901 ccttcttcca ccgccacgcg atcctgcacg agcagaccag cgacgaatac gtgctgccgc
     3961 tgcgccggct ggtttcgaac ccatccgatg tcgccatcat cgactcgctg ctcagcgagg
     4021 aggcggtgat ggccttcgaa tacggctacg ccaccgccga cccgatgacc ctggacatct
     4081 gggaagcgca gttcggcgac ttcgccaatg gcgcgcaggt ggtgatcgac cagttcctcg
     4141 ccgccggtga agcgaagtgg ggccgcctgt gcagcctcgc cctgttcctg ccgcacggct
     4201 acgagggcca ggggccggaa cacagctcgg cgcggctgga gcgcttcctg cagctgtgcg
     4261 cgctcgacaa catgatcgtg tgcgcgccga ccaccccggc gcaggatttc cacatgatcc
     4321 gccggcagat gcgcatgcgc acgcgcaagc cgctggtggt gatgacgccg aagtcgctgc
     4381 tgcgccacaa gctggcggtg tcgagcctgg acgaactcgc gaatggcgag ttccagcggc
     4441 tgatcccgga caccaccgca accgcgaagg ccgtgcgacg cgtcgttgcc tgctccggca
     4501 aggtctacta cgacctgctg gaggaagcgc agcggaacgg gatcgacgac gtcgccctgg
     4561 tccgcgtcga acagctgtat ccgttcccgc gccaggaact ggcggcggaa ctgcagcgct
     4621 tcgctgccgc gaccgaagtc gtctggtgcc aggaagagcc gcagaaccag ggtgcgtggt
     4681 accagatcca gcaccacctg cgcgcctgcc tgcagcccgg gcagtcgctc agctatgccg
     4741 gccgcgcgcg ttcgccctcg cccgccgccg gccacatggc cgagcacgtg gaagagcaca
     4801 gcgcactggt cgccgatgcg ctggtgaacc cgatcgccgg cgaaatctgc ccggaataag
     4861 ccaccacctc ccctttcaac accgcattcc cgacaggacg cccgcatggc caccgaagtc
     4921 aaagtcccgg ttctccccga atccgtttcc gacgccacca tcgccagctg gcacaagaag
     4981 gccggcgacg cggtccgccg cgacgagaac ctgctcgacc tcgagaccga caaggtcgtg
     5041 ctggaagtcc cctcgccggt cgacggcgtg ctcaaggaga tccgcttcga ggccggcgcc
     5101 accgtcaaca gccagcaggt ggtcgcggtg atcgaggaag gcgcggttgc cgccgcccca
     5161 gccgccgccg cagcggcacc cgcagcggcg cctgccgccg cggctccggc cgcaccggct
     5221 tccgctcccg ccagcggcgc gctgcccccg ggtgcgcgct tcgccgccac caccagcggc
     5281 gtggacccgg ccgacgtggc cggcaccggc cgccgcggca tggtcaccaa ggaagacatc
     5341 gtcaactacg ccagtggcaa gaccgccggc gtggccggcg gcgcgcgccc ggaagaacgc
     5401 gtgccgatga cccgcatgcg cgcccgcatc gccgaacgcc tgatgcagtc gaagaactcc
     5461 atcgcgatgc tgacctcgtt caacgaggtg aacctggcca aggtcatgca gatgcgcaag
     5521 gaacttggcg agcagttcga gaaggccaac ggcatcaagc tcggcttcat gggcttcttc
     5581 gccaaggccg cggcgaacgc attgcagaag tacccggtgg tcaatgcatc ggtcgatggc
     5641 aacgacgtca tctaccacgg ctacgccgac atctcggtgg cggtgtccac cgacaagggc
     5701 ctggtcacgc cggtgctgcg caacgtcgag cggatgagct tcgccgacgt cgaatccggc
     5761 atcgccacct atgcgaaggc ggcgcgcgag ggcggcctga agctcgaaga cctgcagggc
     5821 ggcaccttca ccatcaccaa cggcggcacc ttcggctcgc tgatgtcgac cccgatcgtc
     5881 aacccgccgc agagcgcgat cctgggcatg cacgcgatca aggagcgcgc catcgtcgag
     5941 aacggccagg tgatcgccgc gccgatgatg tacatcgcgc tgagctacga ccaccgcatc
     6001 atcgacggca aggacgcggt gctgttcctg gtcgacatca agaaccagct cgagaatccg
     6061 caccggatgc tgctgggcat gtgattgcac gaagccctgc gcaagcgggg cttctcgttt
     6121 caacgcaaag gacacctgca atgagcgaag tacccgcctc tcaacaacaa gccttcgacg
     6181 tcgtcgtcat cggtgccggc cccgccggct accacgccgc catccgcgcc gcgcagctgg
     6241 gcctgaagac cgcctgcatc gacgccgcac tgggcaagga cggcaagccg gcgctgggcg
     6301 gcacctgcct gcgggtgggc tgcatcccgt ccaaggcgct gctggattcc agccgccagt
     6361 tctggaacat gggccacctg ttcgaccagc acggcatcag cttcgagacg cctgcgatcg
     6421 acgtgaagaa gatggtcgcc cgcaaggacg gcatcgtgaa gcagttcacc ggcggcatcg
     6481 ccgcgctgtt caaggccaac aagatcaccg cgttccacgg cttcggccag ctgcagccgg
     6541 gcaacgtggt cagcgtcaag cagcacgacg gcagcactgt cgagctgaag ggcacaaacg
     6601 tcatcctcgc cgccggttcg gattccatcg agctgccgtt cgccaggttc ggcgagcaca
     6661 tcatcgacaa cgtgggcgcg ctggatctgg agaaggtgcc gaagcgcctg ggcgtgatcg
     6721 gcgccggcgt gatcgggctg gaactgggca gcgtgtggaa gcgcctgggc gccgaggtca
     6781 ccatcctcga aggcctgccg accttccttg ccgccgccga cgccgaagtg ggcaaggtcg
     6841 ccgcgcgcga gttcaagaag cagggcctgg acatcaagct gggctgcaag tgcaccgcca
     6901 ccgaggtcaa gaaggacggc gtgcacatca gctacaccga cgacaagggc gccgcgcagg
     6961 agctggtggt cgacaagctg ctggtggccg tgggccgccg cgccgccacc aagggcctgc
     7021 tggccgaagg caccggcgtc aagctcaacg agcgcggcca gatcgaggtc gacgcgcatt
     7081 gccacaccgg cgtcgatggc gtgtgggcgg tcggcgactg cgtgcgcggg ccgatgctgg
     7141 cgcacaaggg cttcgaggaa ggcatcgcgg tggccgagct gatcg
//
LOCUS       DAORUK010000107         7083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_12107_length_7083_cov_21.639727, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000107 DAORUK010000000
VERSION     DAORUK010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12107_length_7083_cov_21.639727"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..1055
                     /locus_tag="PK818_07795"
     CDS             45..1055
                     /locus_tag="PK818_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQY82667.1"
                     /translation="MREIRVAGTLLLAGMLLAGCERQSGSEQAAEAVAPVAAPAAGLA
                     DTASADELRQLAGAAMQAQRLFAPARGNALEYYLALRRRAPADASAQAALVDLQPQLV
                     IAVEQAIATGRYPEAQRLLGLLQQVDAGAPALQRLRESVASAEAAARDLELAMDAESK
                     ARALQAEVELRRQAEDSAAKLASARNPATPAEPVAAATPAAPTPAQSRPLAVAPLAAA
                     AAPAATVAGEAVARQPEPPRPAPASAPVRSAASAAPRVLRQAAPRYPEAALRSRQSGQ
                     VQVAFTIDGDGNVEAPRVVASDLPNAFERAALAAVSRWKFEARGTQHATTTTVRFDPP
                     SS"
     gene            complement(1131..3563)
                     /gene="lnt"
                     /locus_tag="PK818_07800"
     CDS             complement(1131..3563)
                     /gene="lnt"
                     /locus_tag="PK818_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HQY82668.1"
                     /translation="MLASALLLGLYARGGNGYLLGFVALVPWLLALDARAGLGRALLG
                     GLALAIAFAAAGFAWFGAAIGEFSGLGSAGGMLVLLLAAPLLQPQLLAFAIVRELAGR
                     RHGPLVRAFAAAGAWVGCEWLLPKLLGDTLGHGLYPSLPLRQLADLGGAAGLSVLLLL
                     ANEGIAAAIRNRAGGRPGCWRPLALVAALVLAWLGYGGWRAATVAAHAATGDPLRVAM
                     VQTNIVDYERLRRERGAYAVVREVLDTHFAMSRAAVAQADADALLWSETVYPTTFGHP
                     RSADGAALDAEIRDFIASVRTPLVFGSYDLDDAGEYNAAAFLAPDGRLLGMYRKSNPF
                     PLTEYVPAWLDGPGFRRWLPWTGRWQRGDGARVFPLRLADGREVPVLPLICLDDVDPL
                     LAIDGARLGAQAIVGMSNDSWFSAHAQGAELHLAVAAFRSIETRLPQFRVTANGISAA
                     IDPAGTRVATTAIGQPALLTVALPVQPAPRTLVVAWGNWVGAAALAGLLLLAVLDLWV
                     WWRDRRGTPVARAAAAAPALRQLRVVLLPPALRGVLGLLQVIGVGTLAWIGWRMLGRD
                     GLQVNSLAQITLFGSGVVLPALAAGCVRRWWTATVQVDAGALVLVRRRQRVAVPVSGI
                     VAIAPWTLPIPMPGITLRTGPATALSLGFAAGDPAPLAALLRQAGAPAIAGTHASARA
                     LADAHARAAAPRWRIDHRMFKFALFPLLLALPAFRLHQHIAYGGTFGEYHSFGLQAWL
                     LALLIWWASWALGMALYALVLRVLVESGSLATLLLQPPQAAASRVLLQGIARGLYFIG
                     APAWLLWRLLAG"
     gene            complement(3608..4264)
                     /locus_tag="PK818_07805"
     CDS             complement(3608..4264)
                     /locus_tag="PK818_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82669.1"
                     /translation="MPDQDPGERQARPPATGGAGRGRRWLATLLVLVVVGGIGYFLLQ
                     QPPPPPSGGEGPEPAATAPAGADGNPANATSMKPHGTTARRIRAAQDPDDLAAHAIPG
                     EPAPTMGEVIEGLHRAGIRSGLGAFNPPGTSPPLVGLAVPDDYVLPEGYVRHHQATDD
                     GQPIEPILMYSPDYEFVDAAGQPLAIPADRVVPPQHAPPGLPIRQIRIPPPHDPDSPP
                     "
     gene            complement(4280..5371)
                     /locus_tag="PK818_07810"
     CDS             complement(4280..5371)
                     /locus_tag="PK818_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82670.1"
                     /translation="MPVPVCKTARPLLAGLALALLCAPVVASTLNQNVSWTIDRSGTT
                     AKYRVVAYGDSIYAGYNGSTTSAAKYSAPTVDAEYLSALWNADVESVRRTKSGAVASD
                     VYNNKIVAERSYMQASSTRVVTFEMCGNDGLQARSSFKSQTGTCNYSVLATAEANCKT
                     YVAKAMDYINTNAYAGTKVKIIANLHYPGYNADNVLSSCTDASTGARVNMRDKFLPAI
                     TRMNYWMCEYARQKGFKCVDNFAEYMGSDYDSNGDGKVDSQALKYVAGETEASYVARI
                     TGTLKGTLRDANTHFISASSSADYIQSDDTHPTYNGSTVKAGLFGGTTGSGAPRYTSF
                     SGGKNPIWNLYGHERMGWLLSLYGPTSAL"
     gene            5595..6272
                     /locus_tag="PK818_07815"
     CDS             5595..6272
                     /locus_tag="PK818_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQY82671.1"
                     /translation="MTELLETVEHETGPHPQWSVLWLHGLGADGHDFAPIVPELLRRD
                     WPAVRFVFPHAPVRAVTINGGARMRAWYDIRDMNLANRADSEGVAESVAQVEALIARE
                     AERGIGAERLLLAGFSQGGAVTLAAGIARTRPLAGLVALSTYLPMTPAQAQAALQPAA
                     TAQPVFMAHGQYDPVVPCAGGEMSAAALRQFGFDVQWQRYPMQHSVCAEEIGDLGDWL
                     GARFAAG"
     gene            6410..>7083
                     /locus_tag="PK818_07820"
     CDS             6410..>7083
                     /locus_tag="PK818_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005915517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQY82672.1"
                     /translation="MSAGPMRVVIADDEPLARERLRALLAAEPGVQVVAEAGDGHATL
                     HACAEHHPDMVLLDISMPGIDGLETARHLAAFEPRPAVVFCTAYDAHALSAFEAQAID
                     YLVKPVRPERLSAALERVRTFAAGRLQHAEPEDGRKRTHLCARLRGSLRLIPVEDIRY
                     LQAEEKYVVVHHARGEDLIEESLKSLETEFADRFVRIHRNCLVARNEIVELRRIGDGH
                     TQAVLR"
BASE COUNT         1055 a         2492 c         2591 g          945 t
ORIGIN      
        1 catccgccgc atgcagcctg cgcccccaaa gagaggggaa gcacatgcgc gagatcaggg
       61 tggccggcac gttgttgctg gcggggatgc tgctggcggg ttgcgagcgc cagtccggat
      121 cggagcaggc ggccgaagcc gttgcaccgg tagcggcgcc cgcggcgggg ttggcggaca
      181 ccgcgagcgc cgacgaattg cgccagctgg ccggcgcggc gatgcaggcg caacggctgt
      241 tcgcacccgc gcgcggcaat gcgctggagt actacctggc cttgcgccgg cgcgcgcccg
      301 ccgatgccag tgcgcaagcg gcgctggtcg acctgcagcc gcagctggtg atcgcggtcg
      361 aacaggcgat cgccacggga cgctatccgg aagcgcagcg cctgctgggc ctgctgcagc
      421 aagtcgatgc cggcgcgcct gcgctgcagc ggctgcgcga aagcgtggcc agcgccgagg
      481 cggccgcgcg cgatcttgaa ctggcgatgg acgccgaaag caaggcccgg gccctgcagg
      541 ccgaagtcga gttgcgccgg caggcggagg acagcgcggc gaagctggct tcggcgcgca
      601 acccggccac gcctgcggaa ccggtcgccg cggcgacacc tgctgcaccc acgccagcgc
      661 agtcgcgccc gctcgcggtt gcgccgctgg cggctgccgc tgcgccggct gcaaccgtcg
      721 ccggcgaagc cgtcgctcga caaccggaac cgccacgccc ggcgccggcc agcgcgcccg
      781 tgcgtagcgc agccagcgcc gcgccgcggg tgctgcgcca ggccgcgccg cgctatccgg
      841 aggctgccct gcgctcgcgc caatccggcc aggtgcaggt ggccttcacc atcgatggtg
      901 atggcaacgt cgaagcgccg cgggtggttg cctcggacct gcccaacgcg ttcgagcgcg
      961 ccgcgctggc cgcagtctcg cgctggaaat tcgaagccag gggcacccag cacgccacca
     1021 cgaccacggt gcgcttcgac ccgccgtcgt cctgatcgca atccgcgccg aagctgactg
     1081 cagggtcggc gatggccacg cgtgaggcgt cgcccgcggg ggccgcgcgg ctagcctgcg
     1141 agcagccgcc acagcagcca cgccggggcg ccgatgaagt aaagaccgcg cgcgatcccc
     1201 tgcagcagca cgcgactggc ggccgcctgc ggcggctgca gcagcagcgt cgccagcgat
     1261 ccggattcga ccagcacgcg caacaccagc gcatacaggg ccatgccgag cgcccatgac
     1321 gcccaccaga tcagcagcgc cagcagccag gcctgcaggc cgaagctgtg gtactcgccg
     1381 aaggtgccgc cgtaggcgat gtgctggtgc aggcggaacg cgggcagcgc gagcagcagc
     1441 gggaacagcg cgaacttgaa catgcggtgg tcgatgcgcc agcgtggtgc cgcggcgcgc
     1501 gcatgtgcgt ccgccagcgc gcgcgcgctg gcgtgggtgc cggcgatcgc cggcgcgccc
     1561 gcctgccgaa gaagcgccgc cagcggcgcc ggatcgccgg ctgcgaagcc aagcgacagt
     1621 gcggttgccg ggccggtgcg cagggtgatg cccggcatcg ggatcggcag cgtccacggc
     1681 gcgatcgcga cgatgccgct gaccgggacc gccacccgtt ggcggcggcg aaccagcacc
     1741 agcgcaccgg cgtccacctg cacggtggcc gtccaccagc gccgcacgca cccagcagcc
     1801 agcgccggca acaccacgcc ggacccgaac agggtgatct gcgccagtga attcacctgc
     1861 agcccatcgc gcccgagcat gcgccagccg atccatgcca gggtgcccac gccgatcacc
     1921 tgcagcaggc ccagcacgcc acgcagcgcg ggcggcagca gcaccaccct gagctgtcgc
     1981 agggccggtg ccgcggcggc agcgcgcgcc accggagtgc cgcggcggtc gcgccaccag
     2041 acccacagat cgagcaccgc cagcagcaac aggccggcaa gcgcggccgc accgacccag
     2101 ttgccccacg ccaccaccag cgtgcgtggt gctggctgca ccggcagcgc caccgtcagc
     2161 agggccggct ggccgatcgc ggtggttgcc acccgggtgc cggccgggtc gatcgccgcg
     2221 ctgatcccgt tcgcggtcac ccggaactgc ggcaagcggg tttcgatgct gcggaacgcg
     2281 gccaccgcca ggtgcagctc ggcgccctgc gcatgcgcgc tgaaccacga atcattggac
     2341 atgccgacga tcgcctgcgc gccgaggcgc gcgccatcga tcgccagcag cgggtcgacg
     2401 tcgtccaggc agatcagcgg cagcaccggg acctcgcggc cgtcggccag ccgcagcggg
     2461 aacacgcgcg cgccatcgcc gcgctgccag cggccggtcc acggcagcca gcggcggaag
     2521 ccaggcccat ccagccacgc cggcacgtat tcggtcagcg ggaacggatt ggactttcgg
     2581 tacatcccca gcagccggcc atccggcgcc aggaacgccg ccgcgttgta ttcgccggcg
     2641 tcgtcgaggt cgtagctgcc gaacaccagc ggggtccgga cgctggcgat gaagtcgcgg
     2701 atctcggcgt ccagcgccgc gccatccgcg ctgcggggat gcccgaaggt ggtcgggtac
     2761 acggtttccg accacagcag ggcatcggca tccgcctgcg cgaccgccgc ccgcgacatc
     2821 gcgaagtggg tgtcgagcac ctcgcgcacc accgcgtacg ccccgcgttc gcggcgcagg
     2881 cgttcgtagt cgacgatgtt ggtctgcacc atcgccaccc gcagcggatc gccggtggcg
     2941 gcgtgggcgg cgaccgtggc cgcccgccag ccgccgtagc ccagccaggc gagcaccagc
     3001 gccgccacca gcgcgagcgg tcgccagcat ccggggcggc cgccggcgcg gttgcggatc
     3061 gccgccgcga tgccctcgtt ggccagcagc agcagcacgc tcaggccggc ggcgccaccg
     3121 aggtcggcca gctggcgcaa cggcagcgag ggatacaggc catggccgag ggtgtcgccc
     3181 agcagcttcg gcagcagcca ttcgcagccc acccacgcgc cggcggccgc gaacgcgcgc
     3241 accagcggtc catgccggcg cccggccagt tcgcgcacga tcgcgaacgc cagcaactgc
     3301 ggttgcagca gtggcgccgc cagcagcagc accagcatcc cgccggcgct gccgaggccg
     3361 ctgaattcgc cgatcgcggc cccgaaccag gcgaacccgg cggcggcgaa ggcgattgcc
     3421 agcgcgagcc cgcccagcaa cgcgcggccg aggccggcgc gggcatcaag ggcgagcagc
     3481 cacggcacca gcgcgacgaa accgagcagg tagccgttgc cgccgcgcgc gtacagccca
     3541 agcagcagcg cgctggccag cacgcccgcc ggcagccgcc agccggggcg cagggatcga
     3601 agcaggctca gggcggggaa tccgggtcgt gcggcggcgg gatgcggatc tgccggatcg
     3661 gcaggcccgg cggcgcatgc tgcggcggca ccacccggtc ggcgggaatc gccagcggtt
     3721 ggccggcggc gtcgacgaat tcgtagtcgg gcgaatacat caggatcggc tcgatcggct
     3781 ggccgtcgtc ggtggcctgg tggtggcgga cgtagccttc cggcaacacg tagtcatcgg
     3841 gcaccgccag cccgaccagc ggcggactgg taccgggcgg gttgaacgca cccaggccgc
     3901 tgcggatgcc ggcccggtgc aggccttcga tcacctcgcc catcgtcggc gcgggttcgc
     3961 cgggaatggc atgggcggcc aggtcatccg gatcctgcgc ggcgcggatg cggcgcgcgg
     4021 tggtgccgtg tggcttcatc gaggtcgcat tggcagggtt gccgtcggcg cctgccgggg
     4081 ccgttgccgc cggctccggc ccctcgccgc cgctgggtgg cggcggcggt tgctgcagga
     4141 ggaaatagcc gatgccgccg acgaccacca gcaccagcag ggtcgccagc cagcggcggc
     4201 cgcggccagc gccgccagtg gcgggcggcc tggcctggcg ttcgccgggg tcctggtcgg
     4261 gcatgccggc gacgcggggt tacagcgcgg aggtcggccc gtacagcgac agcagccagc
     4321 ccatgcgctc atggccgtac aggttccaga tcgggttctt gccgccgctg aagctggtgt
     4381 agcgcggcgc gccggaaccg gtggtgccgc cgaacaggcc ggccttgacg gtgctgccgt
     4441 tgtaggtcgg gtgggtgtcg tcggactgga tgtagtcggc gctgctgctg gcggagatga
     4501 agtgggtgtt ggcatcgcgc agggtgccct tcagggtgcc ggtgatgcgc gccacgtagc
     4561 tggcctcggt ttcgccggcc acgtacttca gggcctgcga atcgaccttg ccgtcgccgt
     4621 tgctgtcgta gtccgaaccc atgtattcgg cgaagttgtc cacgcacttg aagcccttct
     4681 ggcgcgcgta ttcgcacatc cagtagttca tgcgggtgat cgccggcagg aacttgtcgc
     4741 gcatgttgac gcgggcgccg gtgctggcgt cggtgcagga gctcagcacg ttgtccgcgt
     4801 tgtagccggg gtaatgcagg ttggcgatga tcttgacctt ggtgccggcg taggcattgg
     4861 tgttgatgta gtccatcgcc ttggcgacgt aggtcttgca gttggcctcg gcggtcgcca
     4921 gcacgctgta gttgcaggtg ccggtctgcg acttgaagct ggagcgtgcc tgcaggccgt
     4981 cgttgccgca catctcgaag gtgaccacgc gggtcgagct cgcctgcatg taggagcgct
     5041 cggcgacgat cttgttgttg tacacgtccg acgccaccgc gcccgacttg gtgcggcgca
     5101 cgctttcgac gtcggcgttc cacagcgcgg acaggtattc ggcgtccacc gtcggcgccg
     5161 agtacttggc ggcgctggtg gtggaaccgt tgtagccggc gtagatcgaa tcgccgtagg
     5221 cgaccacccg gtacttggcg gtggtgccgg aacggtcgat ggtccacgac acgttctggt
     5281 tcagggtgct ggccacgacg ggcgcgcaca gcagcgccag cgccaggccc gcgagcagcg
     5341 gacgagcggt cttgcagaca ggtacaggca tggatcagtc cccaggtggt gtatcgaagg
     5401 tggtgccggc cggtggccgg ggcacgatgg gtagcaacca caaggaccat ccggcagccg
     5461 gcctaccccg ccgtccccgc tgccggaccg tacgctagcg tctagtccgg cccgggtcat
     5521 cttgcggcgc agcacgttta gcgtgtgacg cagcgcaccc ttagactgca gcccatcccc
     5581 catcctgcca agccatgacc gaactgctgg aaaccgtcga acacgaaacc ggcccgcacc
     5641 cgcagtggag cgtgctgtgg ctgcatggcc tcggtgccga tggccatgat ttcgcgccga
     5701 tcgtgccgga gctgctgcgg cgcgactggc cggcggtccg cttcgtgttc ccgcatgcgc
     5761 cggtgcgcgc ggtgaccatc aacggcggtg cacgcatgcg cgcctggtac gacatccgcg
     5821 acatgaacct cgccaatcgc gccgacagtg aaggcgtggc cgaatcggtg gcgcaggtgg
     5881 aggcgctgat cgcgcgcgag gccgaacgcg ggatcggtgc ggaacgcctg ctgctggccg
     5941 ggttctcgca gggcggcgcg gtcacccttg ccgccggcat cgcgcgcacc cgcccgctgg
     6001 cgggcctggt cgcgctgtcg acctacctgc cgatgacgcc ggcgcaggcg caggccgcgc
     6061 tgcagcccgc ggcaaccgcg caacccgtgt tcatggcaca tggccagtac gacccggtgg
     6121 tgccgtgcgc cggcggcgag atgagcgcgg cggcgttgcg acagttcggc ttcgacgtgc
     6181 agtggcagcg ctatccgatg cagcattccg tctgcgccga ggagatcggc gacctcggcg
     6241 actggctggg ggcgcggttc gccgccggct gagatgagcg ccgcagcgcc tgcattgccc
     6301 caagtgcgtt ggtcgttcgc cagtcgcaac cgccacggat gaccggcggc tcgggcgggg
     6361 gctactatca cgccgagatc cgggtgccgc tggacgggag tacggcgaca tgagcgcagg
     6421 accgatgcgg gtggtgatcg ccgatgacga gccgctggcg cgcgaacgcc tgcgcgcgct
     6481 gcttgccgcg gaacccgggg tacaggtggt ggccgaggcc ggcgatggcc atgccaccct
     6541 gcacgcctgc gccgaacacc atccggacat ggtgttgctg gacatctcga tgccgggcat
     6601 cgacggcctc gagaccgcgc gccacctggc cgcgttcgag ccgcgcccgg cggtggtgtt
     6661 ctgcaccgcg tacgacgcac atgcgttgtc cgcgttcgag gcacaggcga tcgactacct
     6721 ggtcaagccg gtgcgcccgg agcggctgtc cgccgcgctg gaacgggtgc gcaccttcgc
     6781 cgccgggcgc ttgcagcatg cggaacccga ggacgggcgc aagcgcaccc acctgtgcgc
     6841 gcgcctgcgt ggcagcctgc ggctgatccc ggtcgaggac atccgctacc tgcaggccga
     6901 ggaaaaatac gtggtggtcc accacgcgcg cggcgaggac ctgatcgagg aatcgctgaa
     6961 gtcgctggaa accgaattcg ccgaccgctt cgtgcgcatc caccgcaatt gcctggtggc
     7021 gcgcaacgag atcgtcgagt tgcgccggat cggcgatggc catacccagg cggtgttgcg
     7081 gca
//
LOCUS       DAORUK010000108         7081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_12114_length_7081_cov_20.723740, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000108 DAORUK010000000
VERSION     DAORUK010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12114_length_7081_cov_20.723740"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1030)
                     /locus_tag="PK818_07825"
     CDS             complement(41..1030)
                     /locus_tag="PK818_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQY82673.1"
                     /translation="MSQALGAFDPPRWLRGPHLQSVLGSSPLRAIRARARLRACAAHH
                     QDVVLEAGPGVRLHGVHSARPGFDPRGLVVLLHGWEGSVDSNYMRLTAARLLDAGFAV
                     FRLNFRDHGDSHHLNEGLFHSNRIDEIVEAVRAIAARWPQRPLFAAGYSLGGNFALRL
                     ALRAHSHGLPLQRVVAVCPLLDPARAMAAMETGLPLYIRYFERKWRRSLVRKRDLFPH
                     RHGFADDVLRLRLRALTEWMVLRHTDFATLDDYFEGYSVAGERLRGLPIPADILMSAD
                     DPVIPLRDFEQVAGFPNVQLELAAHGGHCGFIENASLDGYAERWVAARLAAAV"
     gene            complement(1038..1814)
                     /locus_tag="PK818_07830"
     CDS             complement(1038..1814)
                     /locus_tag="PK818_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQY82674.1"
                     /translation="MSATTSAAEPLLRVLRTACRLPLLAWHLLVHLPVTLLLIAIDTV
                     GGKRVGHLAIRWWARGLLWVFGMRVRRIGTPLPGGTMFVANHLGWIDIVALHSQHMMG
                     LIAKAEIRGWPLIGWLARAGESIFLQRGNAHSLAGVMAEMAQRLRDGHAVGVFPEAGT
                     RDGRELGPFHARIFTAAVEADAPVQPVALCFGQRCEAQQIVAFGPGENFLANFVRLLG
                     EPVRPMKVCFLEPILHAEHEGRRRIATLARARIEQAMAAA"
     gene            complement(1954..2568)
                     /locus_tag="PK818_07835"
     CDS             complement(1954..2568)
                     /locus_tag="PK818_07835"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HQY82675.1"
                     /translation="MSITLNFELNDRDLAHFQAAMERSQKAAEGKSDEEIVNCAIALL
                     SDAAKVHIPDFIKDRLLRLDDMIAMVRDDAWALPEADKQRVLSALMYFCDPSDVIPDH
                     VEVLGFLDDAVMIELSVRELKHELDAYDDFCDFRSHEAGRRGVEAATLGRTDWLDGRR
                     EELIDRMHQRRERDFGTGYGGSSGYAHSRGSYVRGWRPGVFKFG"
     gene            complement(2665..4536)
                     /locus_tag="PK818_07840"
     CDS             complement(2665..4536)
                     /locus_tag="PK818_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HQY82676.1"
                     /translation="MLVLGGCASLTPVQRDAAAGVAVAARSTHVDCTRADACALASPL
                     HGMGEHALAESTAAAPRHHALILDGGPDALLARIHLIRAARRSIDLQTYIFDEDDSAQ
                     LVLDELQAAAFRGVRVRLLIDQLSALEKVSTLAAFAAVHANFELRVYNPVLDRGRLST
                     PMYAVAAACCWGKLNRRMHNKLLLVDGMLGITGGRNYQDDYYDWNAAYNFRDRDLLLA
                     GPVAADMGANFEAFWQSPLSVPAERLGDVARYLRTRGVPPLPHQPYRKPERVQALLAQ
                     VEDPDLVRARFVDAALAVQGVQFIGDLPDKHAGAAGGAAPAGDTLQQLIASAQAEVLL
                     QTPYLVLSKPAQQVFRALHRQPAPPRVRVSTNSLASTDAFIAYAISHKYKRRYLRDFG
                     FEIHEFKPFPAAVPFDAGPVGADLGAPPATAASAPTRRARQLAERGLAREPVSEAGRS
                     SPFASPGGLPVRLQRAGMRMGLHAKSLVLDERVGVVGTHNFDPRGDNLNTESAVVIAD
                     PAFARQLAASIRGDMAPANAWTIGRRDSSPILPGIEFTLARVSERMPIFDLWPVKYAT
                     SYQFQAGPDCPSPSSPFAPDFRRCHEPVGDFPEVDIGLKWLGVRVFTAFGSGLSPIL"
     gene            complement(4654..5127)
                     /locus_tag="PK818_07845"
     CDS             complement(4654..5127)
                     /locus_tag="PK818_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQY82677.1"
                     /translation="MRELILLRHAHAEPATAGQDDLGRGLSETGRAEAAAAGDWLRQH
                     GLRPDRVLCSTANRTRETLAALGDIGGGEVHEDASIYEGSAGTLAALADAHRDAERLL
                     LVGHNPGLEQLAALMHSGQTGDYRGMPPGAIAVLALPVDANIEPGIATLTEFWWP"
     gene            complement(5150..5779)
                     /locus_tag="PK818_07850"
     CDS             complement(5150..5779)
                     /locus_tag="PK818_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HQY82678.1"
                     /translation="MKTWLVACSKGGVGKTTVATHLAAEAAVAGLHTALVDADPQESA
                     TRWCQRRAGMASAVLPLDGTRKNWRKALPSDTQRVIIDAPAGAMERDLEDFLDIADAV
                     VVPIQPSALDIEASVRFVESLGKHPRVRKGKLRVGLLGNRLKPHTNASQQALEILRSW
                     PFPLVAQLRDSQAYVVLTGLGKSLFDYHSAQVREHQADWVPLLHWLKKD"
     gene            complement(5839..>7081)
                     /locus_tag="PK818_07855"
     CDS             complement(5839..>7081)
                     /locus_tag="PK818_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY82679.1"
                     /translation="LSNAIKFTEHGSVTAALELRKHDPADGALGSDQLVLRVTDTGIG
                     VSEQAQARLFQPFSQAEADTTRRFGGTGLGLAISRRIAELMGGGVEMESVPGTGTTMR
                     LAIKLHRAPPEEVPEAAGPGRSSPGFNPRRLPTPAEAERERSLVLLVDDHATNRQVIQ
                     RQLALAGYASETADDGVEGLARWRSGRYSLLLSDVHMPHMDGYQLARAIREEEAQRGL
                     PHTPIVALTASALKGEAERCLTAGMDDYMAKPVGIATLGACLQRWLPHTAGAAPVPAA
                     IGASIQAANNQRIIAREAGLPQLAHPPALDPAVLADLTAGDAADTRALLDDFLGSTGE
                     DLAQLEALRSGGDLHGMTRQAHKIKGAARLVGALELAAAAEVLESAGRAGDWASVLPL
                     AVDVATAVARLRLDVVERWPG"
BASE COUNT         1032 a         2529 c         2520 g         1000 t
ORIGIN      
        1 ttcactctgg ccccttttcc cggacgcaca cacggtctcg ctagaccgct gcggccaggc
       61 gcgcggccac ccagcgctcc gcataaccat ccagcgacgc gttctcgatg aagccgcaat
      121 ggccgccatg cgcggccagt tccagttgca cgttcgggaa gcccgccacc tgctcgaagt
      181 cgcgcagcgg gatcaccggg tcgtcggcgg acatcaggat gtcggccggg atcggcaacc
      241 cgcgcaggcg ttcgccagcc accgaatagc cctcgaaata gtcgtcgagg gtggcgaagt
      301 cggtgtgccg cagcaccatc cattcggtca gcgcgcgcag gcgcagcctg agcacgtcgt
      361 cggcgaagcc gtggcggtgc gggaacaggt cgcgcttgcg caccagcgag cgccgccact
      421 tgcgttcgaa gtagcggatg tacagcggca gcccggtttc catcgccgcc atcgcccgcg
      481 ccgggtccag cagcgggcag accgcgacca cgcgctgcag gggcaggcca tggctgtgcg
      541 cgcgcaaggc caggcgcagc gcgaagttgc cgcccagcga atagccggcg gcgaacagcg
      601 gccgctgcgg ccagcgcgcg gcgatcgcgc gcaccgcctc cacgatctcg tcgatccggt
      661 tggaatggaa caggccttcg ttgaggtggt ggctgtcgcc gtggtcgcgg aaattgagcc
      721 ggaacaccgc gaacccggcg tcgagcagcc gcgccgcggt gagccgcatg tagttggaat
      781 cgacgctgcc ttcccagccg tgcagcagca ccaccaggcc gcgcggatcg aacccgggcc
      841 gcgcgctgtg cacgccgtgc aggcgcaccc cgggacccgc ctcgaggacc acatcctggt
      901 ggtgtgcggc gcaggcgcgc aggcgcgcgc gcgcacggat cgcgcgcagc ggactggacc
      961 cgagcaccga ttgcaggtgc gggccgcgca gccagcgcgg cgggtcgaat gcgccgaggg
     1021 cttggctcat cgcgcgctca cgcggcggcc atcgcctgct cgatgcgcgc acgcgcgagc
     1081 gtcgcgatgc ggcgccggcc ctcgtgctct gcgtgcagga tcggctccag gaagcacacc
     1141 ttcatcggcc gcaccggttc gcccagcagc cgcacgaaat tggccaggaa attctcgccc
     1201 gggccgaagg ccacgatctg ctgggcctcg caacgctggc cgaagcacag cgcgaccggc
     1261 tgcaccgggg cgtcggcctc gaccgcggcg gtgaagatgc gggcgtggaa cggacccagt
     1321 tcgcggccat cgcgggtgcc ggcttccggg aacaccccca ccgcatggcc atcgcgcagc
     1381 cgttgcgcca tctccgccat caccccggcc agcgagtgtg cgttgccgcg ctgcaggaag
     1441 atgctttcgc ccgcgcgcgc cagccagccg atcagcggcc agccgcggat ctcggccttg
     1501 gcgatcaggc ccatcatgtg ctgcgagtgc agggcgacga tgtcgatcca gcccaggtgg
     1561 ttggcgacga acatggtgcc gccgggcagc ggggtgccga tccggcgcac ccgcatcccg
     1621 aacacccaca gcaggccgcg cgcccaccag cggatcgcga ggtgcccgac ccgcttgccg
     1681 ccgacggtgt cgatcgcgat cagcagcagg gtgaccggca ggtgcaccag caggtgccag
     1741 gccagcagcg gcaggcggca ggcagtgcgc aggacgcgca gcagcggttc ggcggcggag
     1801 gtcgtcgcac tcatggcctg gacctggcga agcgcgctgg actgcagggc ccgcccgcgc
     1861 ccaggacgcg accgctgcgg aaggcggccg cgcgccggtg cgggcgcgca tccggcaggc
     1921 gacgcatccg cggcgcgggc gcggtgcccg tgatcagccg aacttgaaca cgcccgggcg
     1981 ccagccgcgg acgtacgaac cacggctgtg cgcgtaaccg ctgctgccgc cgtagccggt
     2041 gccgaaatcg cgctcgcggc gctggtgcat gcggtcgatc agttcctcgc gacggccatc
     2101 cagccagtcg gtgcggccca gcgtggccgc ttccaccccg cgacggcccg cctcgtggga
     2161 acggaaatcg cagaagtcgt cgtaggcgtc cagctcgtgc ttcagctcgc gcaccgacag
     2221 ctcgatcatc accgcgtcgt cgaggaagcc cagcacctcg acatggtcgg ggatcacgtc
     2281 gctcgggtcg cagaagtaca tcagcgccga gagcacccgc tgcttgtcgg cctccggcag
     2341 cgcccacgca tcgtcgcgca ccatcgcgat catgtcgtcg aggcgcagca ggcggtcctt
     2401 gatgaaatcg gggatgtgca ccttggccgc atccgagagc agcgcgatcg cgcaattgac
     2461 gatctcctcg tcgctcttgc cctcggccgc cttctgggaa cgctccatcg ccgcctggaa
     2521 atgggcgagg tcgcggtcgt tgagctcgaa attcagggtg atcgacatgg cggcgtcctg
     2581 gcacgcgaaa tgagaccggc agactaggcc gcgcgcagcg gcgcgtcaat ccgacgcagc
     2641 cggggcccgg gagcggccac gccgctacag gatcggcgac aaccccgacc cgaacgcggt
     2701 gaatacccgc acccccagcc acttcaggcc gatgtccact tccgggaagt cgcccaccgg
     2761 ttcgtggcag cggcgaaagt cgggggcgaa cggcgacgac ggcgacgggc aatcgggacc
     2821 ggcctggaac tggtagctgg tcgcgtactt gaccggccac aggtcgaaga tcggcatccg
     2881 ctcggaaacc cgggccaggg tgaactcgat cccgggcagg atcggcgagg agtcgcgacg
     2941 gccgatcgtc cacgcgttgg cgggggccat gtcgccgcgg atgctggccg ccagctggcg
     3001 cgcgaacgcg gggtcggcga tcaccaccgc gctttcggtg ttgaggttgt cgccacgcgg
     3061 gtcgaagttg tgggtgccga ccacccccac ccgttcgtcc agcaccagcg acttggcgtg
     3121 caggcccatg cgcatgccgg cgcgctgcag ccgcaccggc agcccgcccg gcgaggcgaa
     3181 cggcgagctg cgcccggcct cgctgaccgg ctcgcgggcc aggccgcgct ccgccagctg
     3241 gcgcgcgcgc cgggttggcg cgctcgccgc ggtcgcaggc ggtgcgccaa ggtcggcgcc
     3301 gaccgggccg gcgtcgaacg gcaccgcggc cgggaatggc ttgaattcgt ggatctcgaa
     3361 gccgaagtcg cgcaggtagc ggcgcttgta cttgtgcgag atcgcgtagg cgatgaaggc
     3421 gtcggtcgaa gccaggctgt tggtggagac ccgcacccgc ggcggcgccg gctgccggtg
     3481 cagggcccgg aacacctgtt gcgccggctt cgacagcacc aggtacggcg tctgcaacag
     3541 cacttccgcc tgcgccgacg cgatcagctg ctgcagggtg tcgccggccg gcgccgcgcc
     3601 gccggcggcc ccggcgtgct tgtccggcag gtcgccgatg aactgcacgc cctgcaccgc
     3661 cagcgccgca tccacgaagc gtgcgcgcac caggtcgggg tcctcgacct gcgccagcag
     3721 ggcctgcacc cgttccggct tgcggtaggg ctggtgcggc agcggcggca ccccgcgcgt
     3781 gcgcaggtag cgggccacgt cgcccagccg ctccgccggc acgctcagcg gcgactgcca
     3841 gaacgcctcg aaattcgcgc ccatgtccgc cgccaccggg ccggcgagga gcaggtcgcg
     3901 gtcgcggaag ttgtaggccg cgttccagtc gtagtaatcg tcctggtagt tgcggccgcc
     3961 ggtgatgccc agcatcccgt ccaccagcag cagcttgttg tgcatgcgcc ggttgagctt
     4021 gccccagcag caggctgcgg cgaccgcgta catcggggtc gacaggcggc cgcggtccag
     4081 caccgggttg tacacgcgca gttcgaagtt cgcgtgcacg gcggcgaacg cggccagggt
     4141 ggagaccttc tccagcgccg acagctggtc gatcagcagc cgcacccgca cgccgcggaa
     4201 tgcggccgcc tgcagctcgt cgagcaccag ctgcgcggaa tcgtcctcgt cgaagatgta
     4261 ggtctgcagg tcgatgctgc ggcgggcggc gcggatcagg tggatgcgcg ccagcaacgc
     4321 gtccggcccg ccatcgagga tcagcgcgtg gtgccggggc gcagccgcgg tggattcggc
     4381 cagcgcgtgc tcgcccatcc cgtgcagcgg cgaggccagc gcgcaggcat ccgcgcgggt
     4441 gcagtcgacg tgggtgctgc gggcggcgac cgcaaccccg gccgcggcat cgcgctgcac
     4501 cggggtgagg ctggcgcagc cgccgaggac cagcacggcc agcgccagca gcaggaaaaa
     4561 ggggccagag tgaatttccc gtcgaacatg ctcactggtt cgagaatcct ggtcgggaaa
     4621 cttcactctg gccccttttt ccccatcatc gattcacggc caccagaact cggtcagggt
     4681 ggcgatgccg ggttcgatgt tggcgtccac cggcaacgcc agcaccgcga tggcgcccgg
     4741 cggcatcccg cggtagtcgc cggtctggcc gctgtgcatc agcgccgcca actgttcgag
     4801 gccggggttg tggccgacca gcagcagccg ctcggcatcg cggtgggcat ccgccagcgc
     4861 ggccagggtg ccggcgctgc cttcatagat ggaggcgtcc tcgtggactt cgccgccgcc
     4921 gatgtcgccg agcgcggcca gggtctcgcg ggtgcggttc gcggtcgagc acagcacccg
     4981 gtcggggcgc aggccgtgct ggcgcagcca gtcgccggcg gcagccgctt ccgcgcgccc
     5041 ggtttcggac agtccgcggc cgaggtcgtc ctgtccggca gtggcgggtt cggcgtgggc
     5101 gtggcggagc aggatcagtt cgcgcattcg atgtcctttc gagggtggat caatctttct
     5161 tcagccaatg cagcagcggc acccagtccg cctggtgctc gcgcacctgc gcggagtggt
     5221 agtcgaacag gctcttgccg agcccggtca ggaccacgta ggcctggctg tcgcgcagct
     5281 gcgcgaccag cgggaacggc cagctgcgca ggatctccag cgcctgctgc gaggcattgg
     5341 tatgcggttt gaggcggttg ccgagcaggc ccacgcgcag cttgcccttg cgcacccgcg
     5401 gatgcttgcc cagcgattcg acgaaacgca cgctggcctc gatgtccagc gcggacggct
     5461 ggatcggcac caccaccgca tcggcgatgt cgaggaagtc ctccaggtca cgctccatcg
     5521 cccccgccgg ggcatcgatg atcacccgct gggtgtcgga cggcagtgcc ttgcgccagt
     5581 tcttgcgggt cccgtcgagc ggcagcaccg cgctggccat gcccgcgcgc cgctggcacc
     5641 agcgggtggc cgactcctgc gggtcggcat cgaccagtgc cgtgtgcagg ccggcgaccg
     5701 cggcctccgc ggccaggtgg gtggccaccg tggtcttgcc gacgccgccc ttggaacacg
     5761 ccaccaacca ggtcttcatc gtgcgctccg atgcgggatg accgcagcgt acacgcggcg
     5821 gtgggcgcgt gcatggattc agccaggcca gcgctcgacg acgtcaagcc gcaaccgcgc
     5881 cactgcagtc gccacgtcca ccgccagcgg cagcacgctg gcccagtcgc cagcgcgacc
     5941 ggcgctttcc agcacctccg ccgccgccgc cagctccagt gcgcccacca gccgcgccgc
     6001 gcccttgatc ttgtgcgcct gccgggtcat gccgtgcagg tcgccgccgc tgcgcagcgc
     6061 ttccagctgg gccaggtctt cgccggtgct gcccaggaag tcatccaaca gggcgcgggt
     6121 atccgccgca tcgccggcgg tcaggtcggc cagcaccgcc ggatccagcg ctggtggatg
     6181 cgccagctgc ggcagcccgg cctcgcgggc gatgatccgc tggttgttgg ccgcctggat
     6241 cgaggcacct atcgccgccg ggaccggtgc ggcccccgcg gtgtgcggaa gccagcgctg
     6301 caggcaggcg ccaagggtgg cgatcccgac cggcttggcc atgtagtcat ccatgccagc
     6361 ggtgaggcat cgctcggcct cgcccttcag cgcggacgcg gtcagcgcga cgatcggcgt
     6421 gtgtggcagg ccgcgctgcg cctcttcctc gcgaatcgca cgggccagct ggtaaccgtc
     6481 catgtgcggc atgtggacgt cgctgagcag cagcgagtag cggccgctgc gccagcgcgc
     6541 cagcccttcg accccatcgt ccgcggtttc cgaggcgtag ccggccagtg ccagctggcg
     6601 ctggatgacc tggcggttgg tggcatggtc atccaccagc agcaccaggc tgcgctcgcg
     6661 ctccgcctcg gcgggggtgg gcaggcgtcg cgggttgaag ccggggctcg accgtcccgg
     6721 gccagcggct tccggcactt cctcgggcgg cgctcgatgc agcttgatcg ccaggcgcat
     6781 ggtcgtgccc gtcccgggca ccgattccat ctccaccccg ccacccatca gttcggcgat
     6841 gcggcggctg atcgcaagcc cgaggccggt gccaccgaaa cgcctcgtgg tgtccgcctc
     6901 tgcctgcgag aatggctgga acagccgcgc ctgcgcctgc tcgctgaccc cgatgccggt
     6961 atcggtgacc cgcaacacca gctggtcgct gcccagcgca ccgtcggcgg gatcgtgctt
     7021 gcgcagctcc agcgccgcgg tgacgctgcc gtgctcggtg aacttgatcg cgttggagag
     7081 g
//
LOCUS       DAORUK010000109         7033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_12240_length_7033_cov_18.326455, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000109 DAORUK010000000
VERSION     DAORUK010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7033
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12240_length_7033_cov_18.326455"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1097
                     /locus_tag="PK818_07860"
     CDS             42..1097
                     /locus_tag="PK818_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HQY82680.1"
                     /translation="MARPNRSAEIDAALLQAVGQHPRDLVGMVAAQLGLSTARISMQV
                     RALVAGGYLYKLGTTRPTYSLGPNRRFEHTYPRAGLAEDRVWHGDLLPLLERLPRNVL
                     DIAQHGVTEMINNAIDHSEAAYVQVRMQQRDGRLLFEITDAGIGIFRKITRALDLPDE
                     RLALLELSKGKLTTDPQRHSGEGIFFTSRMFDAYRIESGGLVFDHDATHDLDLLDDTH
                     DVAGTRVVMQLATDSPRTVEAVFSQFSSGPDDYAFARTVVPVRLARVGDENLVSRSQA
                     KRLLQRVEHFRHVVLDFAEVARVGQAFADEIFRVFANAHPEVELLAVNALPEVQQMIR
                     RAEVLRDEQGDQLPLLK"
     gene            1109..2011
                     /locus_tag="PK818_07865"
     CDS             1109..2011
                     /locus_tag="PK818_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016849672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQY82681.1"
                     /translation="MNFPDYPFTPRRVEVRPGIAMSFLDEGPRDGEVIVMLHGNPSWS
                     FYWRHLVGGLSDRYRCIVPDHVGMGLSDRPDDAPSASPNYDYSLQSRIEDLDALLKHL
                     GVDGPVTLAVHDWGGMIGFGWALRDPSRVKRLVITNTASFPLPRSKRFPKRLAMGRDS
                     RLGGWLIRRFNLFARGAAWMGTTRSLPKAVRAAYAGVYDGWDNAISTLRFMQDIPLDA
                     GDRAMPLVQAAAAALPGYADRPAFIGWGLRDFVFDKHFLAGFRDALPNAEVHAFEDAN
                     HYVLEDKHEVLVPAIRRFLDEHPL"
     gene            complement(2201..2590)
                     /locus_tag="PK818_07870"
     CDS             complement(2201..2590)
                     /locus_tag="PK818_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007966384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HQY82682.1"
                     /translation="MPVEHPCLTCGACCAFFRVSFHWSEADAALGGAVPFELTEPLRT
                     HERAMRGTSQAQPRCIALDADIGRHSRCTIHDRRPSVCALVPASWEFGAASAQCDKAR
                     LAHGLPVLVPKDWIGVDEGEKNPLPEA"
     gene            2617..4287
                     /locus_tag="PK818_07875"
     CDS             2617..4287
                     /locus_tag="PK818_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031956 - medium-chain fatty acid-CoA
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid CoA ligase family protein"
                     /protein_id="HQY82683.1"
                     /translation="MRETPPATPCNIAAALPRLARERPEQVAMRCPGRGGGYQVAITY
                     AQLDARSDAIAAGLARRGIVRGTRTVVMVRPTPEFFLLMFALFKAGAVPVLVDPGIDR
                     RALKQCLDEARPEAFIGIPLALLAKALLGWAKSARVRITTGRRAWLADATLADVERDG
                     AGAGPQLADTAPDDVAGLLFTSGSTGVPKGVVYRHRHFVAQVAMLRDAFGIEAGGIDL
                     PTFPPFALFDPALGLTSIIPDMDPTRPAKADPRKLHAAIRRFGVTQLFGSPALMRVLA
                     GHGEPLPTVRRVTSAGAPVPPEVVARMLALLPPDAQLWTPYGATECLPVAVIEGRELL
                     ALRERTEAGSGTCVGRPVARNTVRIIRIDDGAIADWSDALQVAPGQVGEITVAGPSAT
                     DAYFNRDAQTALAKIRERLPDGGERIVHRMGDLGWFDGDGRLWFCGRKSQRVVVDAVT
                     TLCTEQVEPVFNAHPGVARTALVGTGAQGMQSPVLCVELNAGIGKREWPRIERELRHL
                     GEGFVHTAKVDTFLRYPKPFPVDIRHNAKIGRETLARWAAARRNGERA"
     gene            4284..5279
                     /locus_tag="PK818_07880"
     CDS             4284..5279
                     /locus_tag="PK818_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014645546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQY82684.1"
                     /translation="MNVLVTGGGGFLGLALCRALLAQGHQVASFSRSRHPALDALGVR
                     QLQGDLADREAVLRAFPEGFDAVLHNAAKAGTWGSHASYFSANVIGTRNVIAACRAHG
                     IGKLVHTSTPSVVHRATHPVEGLGADEVPYGEGVKAHYAATKIIAEREVLAANDAALA
                     TVALRPRLIWGPGDTQILPRLVARARSGRLRLVGGGDNLVDTTYIDNAAQAHVAALDH
                     LHPGAACAGRAYFISNGEPWPLREVINGLLRAAGAPEVRKSIPFRAAYALGAACEGLW
                     TLLPLRGDPPMTRFLAEQLGTSHWYSMAPAARDFGYVPKVSMAEGLERLRRTLHR"
     gene            5437..6096
                     /locus_tag="PK818_07885"
     CDS             5437..6096
                     /locus_tag="PK818_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HQY82685.1"
                     /translation="MSQSPFNWKQPAGKALEAALNHALALDAETRAALRGLDGQRVAL
                     QLTSPPLALQVRVDGEALRVGPLDAAREPDLGVRSTLAGLLGQLPMFRRGDAPPVGKL
                     RIEGDAELARRLQRLARDFDPDWEAPFVRVFGDIAGVQVARALAAGLRQARVAGRNLA
                     ESAAEYVTEESRDVVPRAELEAFHDDVDALRDDVERIAAKIARLQRGMPGNGNGNGAG
                     A"
     gene            6093..>7033
                     /locus_tag="PK818_07890"
     CDS             6093..>7033
                     /locus_tag="PK818_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HQY82686.1"
                     /translation="MKSALRAMRIGRVLLRYRLDDLLDDTPAERWLKLMRPFVPGAAP
                     SIASMPRGARLRLALQELGPIFVKFGQILSTRRDLVPADIAEELTHLQDRVAPFDGGV
                     ARGLVERELGRSIADAYASFDTTPLASASIAQVHAATLHDGREVVVKVLRPRIEVQIA
                     ADIALLKSVAGIVERAHPNADKIRPREIVAEIENTLAAELDLQREAGNASLLRRNWEN
                     SPDLYVPEVIWSHTAEGAMTMERVRGIPSDDIKALDAAGIDRRRLAAKGVRVFYQQVF
                     RDNFFHADAHAGNIWVDATRKQDPRFIALDFGIMGQLS"
BASE COUNT         1051 a         2464 c         2481 g         1037 t
ORIGIN      
        1 cttaactagc aaataagccg gtttgatttt agagtgccgg catggcccgc cccaaccgct
       61 ccgccgagat cgacgccgcc ctgctgcagg cagtgggtca gcacccacgg gacctggtag
      121 gcatggtggc agcgcagctg ggcctgtcca cggcgcggat cagcatgcag gtgcgcgcat
      181 tggtggccgg cggctacctg tacaagcttg ggaccacccg gccaacctat tcgcttggcc
      241 ccaatcgccg cttcgagcac acgtatccgc gcgcgggact ggcggaggac cgggtctggc
      301 atggcgacct gctgcccctg ctggagcgcc tgccacgcaa cgtgctggac atcgcccagc
      361 acggcgtcac cgagatgatc aacaacgcca tcgaccactc ggaagcagcg tacgtgcagg
      421 tgcgcatgca gcagcgcgat ggccggctgc tgttcgagat taccgatgcg ggcatcggca
      481 ttttccgcaa gatcacccgt gcgctggatc tgccggacga acgccttgca ttgctggaat
      541 tgtcgaaagg caagctgacc accgatccgc agcggcacag tggcgagggc atcttcttca
      601 cctcgcgcat gttcgatgca taccggatcg aatccggcgg cctggtgttc gatcacgatg
      661 caacccatga cctggatctg ctggacgata cgcacgacgt ggccggaacc cgggtcgtca
      721 tgcagctggc gaccgattcg ccgcgcacgg tggaagcggt gttcagtcaa ttctccagtg
      781 gcccggacga ctacgccttt gcccgcaccg tggtcccggt ccgcttggcc cgggtgggcg
      841 acgaaaacct ggtatcgcgt tcacaggcca agcgtctgct gcagcgcgtg gagcacttcc
      901 gccatgtggt gctggacttt gccgaggtgg cccgggtggg gcaggcgttt gccgacgaga
      961 ttttccgtgt gttcgcaaac gcccaccccg aggtggagct gctggcggtc aacgcgctgc
     1021 cggaggtgca gcagatgatc cgtcgcgcgg aagtgttgcg tgacgagcag ggcgatcagt
     1081 tgccgctatt gaaatgaaag gagccacatt gaatttcccc gactacccct tcaccccgcg
     1141 gcgcgtcgaa gtgcgccccg gcatcgccat gtccttcctc gacgaaggac cgcgcgacgg
     1201 cgaagtcatc gtcatgctgc acggcaatcc gtcgtggagc ttctactggc ggcacctcgt
     1261 cggcgggttg tcggatcgtt accgctgcat cgtgcccgac cacgtgggca tgggcctgtc
     1321 cgaccggccg gatgacgcgc cgtcggcatc accgaactac gactacagct tgcagtcgcg
     1381 gatcgaagac ctcgatgcgt tgctgaagca cctcggcgtc gacgggccgg tgacgctggc
     1441 ggtgcacgac tggggcggga tgatcggctt cggctgggcg ttgcgcgacc cgtcgcgggt
     1501 gaagcggctg gtgatcacca acaccgcctc gttcccgctg ccgcggtcca agcgcttccc
     1561 gaagcggctg gcgatgggcc gcgattcgcg cctcggcggc tggctgatcc gccgcttcaa
     1621 cctgttcgcg cgcggcgcgg cgtggatggg caccacccgc tcgctgccga aggccgtgcg
     1681 cgcggcgtat gccggcgtct acgacggctg ggacaacgcg atctccaccc tgcgcttcat
     1741 gcaggacatc ccgctggacg ccggcgaccg cgcgatgccg ctggtgcagg ccgcggcggc
     1801 cgcattgccg ggctacgcgg accggccggc cttcatcggc tggggcctgc gggacttcgt
     1861 gttcgacaag catttcctgg ccggcttccg cgacgcattg ccgaatgccg aagtccacgc
     1921 gttcgaagac gccaaccact acgtgctgga agacaagcac gaggtgctgg tgccggcgat
     1981 ccgccgcttc ctcgacgagc acccgctctg accatcgacg acgcggcgac ccgccaacgc
     2041 gacggcggga ccgtcggggt gagcggggaa ttcgagcggc gcagccgcga gcccgtgaac
     2101 gtccttcggc atgcaagccg acgggccggg gcgtagcggc attcagcgcc gcggagcgcg
     2161 tcgcccggcg gtaggcgccg ccgctgcgat aacgctgcgc tcacgcctcc ggcaatggat
     2221 tcttctcgcc ctcgtcgacg ccgatccagt ccttcggcac cagcaccggc agcccatgcg
     2281 cgaggcgcgc cttgtcgcat tgcgcgctgg ccgcgccgaa ctcccagctc gccggcacca
     2341 gcgcgcacac cgacggccgg cggtcgtgga tcgtgcagcg cgaatggcgg ccgatgtccg
     2401 cgtccagcgc gatgcagcgc ggctgcgcct gcgaggtgcc gcgcatcgcg cgctcgtggg
     2461 tgcgcagcgg ctcggtgagt tcgaacggca ccgcgccgcc gagcgcggca tccgcttccg
     2521 accagtggaa gctgacccgg aagaacgcgc agcaggcgcc gcaggtgagg caggggtgct
     2581 cgacgggcat gcgccattgt cggcgacaat gcacgcatgc gcgagacgcc gcccgccacg
     2641 ccctgcaaca tcgccgccgc gctgccgcgg ctggcgcgcg agcgccccga gcaggtggcg
     2701 atgcgttgcc cgggccgcgg tggcggctac caggtcgcca tcacttacgc ccagctcgat
     2761 gcgcgcagcg acgccatcgc cgccgggctg gccaggcgcg ggatcgtccg cggcacccgc
     2821 acggtggtga tggtgcggcc aacgccggag ttcttcctgc tgatgttcgc cctgttcaag
     2881 gccggcgcgg taccggtgct ggtggatccc ggcatcgaca ggcgcgcgtt gaagcaatgc
     2941 ctggacgaag ccaggcccga agccttcatc ggcatcccgc tggcgctgct ggcgaaggcg
     3001 ctgctcggct gggcgaagtc ggcgcgcgtc cgcatcacca ccggccgtcg cgcatggctc
     3061 gccgatgcga cgctggccga tgtcgaacgc gatggcgcgg gcgcggggcc gcaactggcg
     3121 gacacggcgc cggacgatgt cgccggcctg ctgttcacca gcggcagcac cggcgtgccg
     3181 aagggcgtgg tctaccggca ccggcatttc gtcgcccagg tcgcgatgct gcgcgacgcc
     3241 ttcggcatcg aagccggcgg catcgacctg ccgaccttcc cgccgttcgc cctgttcgat
     3301 cccgcgctcg ggctgacctc gatcattccc gacatggacc cgacgcgccc ggcgaaggcg
     3361 gatccgcgca agctgcacgc ggcgatcagg cgcttcgggg tgacccagtt gttcggatcg
     3421 ccggcgttga tgcgggtgct ggccgggcat ggcgagccgc tgcccacggt ccgccgcgtg
     3481 accagtgccg gtgcaccggt gccgccggag gtggtggcaa ggatgctcgc gctgctgccg
     3541 cccgatgcgc agctgtggac cccgtacggc gccaccgaat gcctgccggt ggcggtgatc
     3601 gaaggccgcg agctgctggc cctgcgcgaa cgcaccgagg ccggcagcgg cacctgcgtc
     3661 ggccgcccgg tcgcgcggaa cacggtgcgc atcatccgca tcgatgacgg cgcaatcgcc
     3721 gattggagcg atgcgttgca ggtggcaccg gggcaggtcg gcgagatcac ggtcgccggc
     3781 cccagcgcca ccgacgccta cttcaaccgc gacgcgcaga ccgcgctggc gaagatccgc
     3841 gaacgcctgc ccgatggcgg cgaacgcatc gtccaccgca tgggcgacct tggctggttc
     3901 gatggcgatg gccggctgtg gttctgcggc cgcaagtcgc agcgggttgt cgtcgatgcg
     3961 gtcaccacgc tgtgcacgga acaggtcgag ccggtgttca acgcccaccc cggggtggcg
     4021 cgcaccgcgc tggtcgggac cggcgcgcag ggcatgcaat cgccggtgtt gtgcgtggaa
     4081 ttgaatgccg ggatcggcaa gcgcgaatgg ccgcggatcg aacgcgaact gcgccacctg
     4141 ggcgaaggct tcgtccacac cgccaaagtc gatacattcc tgcgctatcc gaaaccattc
     4201 ccggtcgaca tccgccacaa cgccaagatc gggcgcgaga cgctggcgcg ttgggcggcg
     4261 gcacggcgga acggggagcg cgcgtgaacg tcctggtcac cggcggcggt ggtttcctcg
     4321 gcctggcgct gtgccgcgcg ctgctggcgc aggggcatca ggtggccagc ttcagccgca
     4381 gccggcaccc ggcgctggac gcactgggcg tgcgccagct gcagggtgac ctggccgacc
     4441 gcgaggcggt gctgcgcgca ttcccggagg gcttcgatgc ggtcctgcac aacgccgcca
     4501 aggccggcac ctggggcagc cacgccagct acttcagcgc gaatgtcatc ggcacccgca
     4561 acgtgatcgc ggcttgccgc gcgcacggga tcggcaagct ggtgcacacc tccaccccca
     4621 gcgtggtgca tcgcgccacc catccggtgg agggcctcgg cgccgacgag gtgccgtatg
     4681 gcgagggcgt gaaggcccac tacgcggcca ccaagatcat cgccgagcgc gaagtgctgg
     4741 ccgccaacga tgccgcgctg gccacggtgg cgctgcgccc gcgcctgatc tggggcccgg
     4801 gcgataccca gatcctgccg cggctggtcg cgcgtgcgcg cagcgggcgc ctgcggctgg
     4861 tcggcggcgg cgacaacctg gtcgacacca cctacatcga caacgccgcg caggcgcatg
     4921 tcgcggcgct ggaccacctg caccccggcg cggcctgcgc gggccgcgcc tacttcatca
     4981 gcaatggcga accgtggccg ctgcgcgagg tgatcaacgg cctgttgcgc gcggccggcg
     5041 cgcccgaggt gcgcaagtcc atcccgttcc gcgcggcgta tgcgctgggc gcggcctgcg
     5101 aggggctgtg gacgctgctg ccgttgcgcg gcgatccacc gatgacccgg ttcctggccg
     5161 aacaactcgg caccagccac tggtattcga tggcgccggc ggcgcgcgac ttcggttatg
     5221 tgccgaaggt gtcgatggcg gagggattgg agcggttgcg acgaactctt catcgctgac
     5281 gcaccgcatc ggccggcggc agtgcgcagc cgctccatcc agtcatccac gactgggtcg
     5341 cgggcgcggg ccgtccctgg cccgctctgc gcactgccgc cggccgatgc ggcgctcacg
     5401 acctctgtcg tgaagcgaag cccatacaat gccggcatga gccagtcccc cttcaactgg
     5461 aagcaacccg cgggcaaggc gctggaagcc gcgctcaacc acgcgctggc gctggacgcg
     5521 gaaacccgcg ctgccctgcg cgggctcgac gggcagcgcg tcgccctgca gctgacgtcg
     5581 ccgccgttgg cgctgcaggt gcgggtcgat ggcgaggcgc tgcgggttgg cccgctcgac
     5641 gccgcgcgcg aacccgacct cggcgtgcgc agcaccctgg ccgggctgct cgggcaattg
     5701 ccgatgttcc gtcgcggcga cgccccgccg gtcggcaagc tgcggatcga gggcgacgcc
     5761 gaactggcgc ggcgcctgca acggctcgcc cgcgacttcg acccggactg ggaagcgccg
     5821 ttcgtgcgcg tgttcggcga catcgccggc gtgcaggtcg ccagggcact ggccgcgggc
     5881 ctgcggcagg cgcgggtcgc cggcaggaac ctcgccgaaa gcgccgccga atacgtgacc
     5941 gaggaaagcc gcgacgtggt cccgcgcgcc gagctggagg ccttccacga cgacgtcgat
     6001 gcgctgcgcg acgacgtcga gcgcatcgcc gcgaagatcg cgcgcctgca acgcggcatg
     6061 cccggcaacg gcaacggcaa cggggcgggc gcgtgaagtc cgccctgcgc gcgatgcgca
     6121 tcggccgcgt gttgctgcgc taccggctcg acgacctgct cgacgacacc cccgccgagc
     6181 gctggctgaa gctgatgcgg ccgttcgtgc cgggcgcggc gccgagcatc gccagcatgc
     6241 cgcgcggcgc gcgcctgcgg ctggcgctgc aggagctcgg cccgatcttc gtcaagttcg
     6301 ggcagatcct gtcgacccgc cgcgacctgg tgcccgccga catcgccgag gaactgaccc
     6361 acctgcagga ccgggtggcc ccgttcgatg gcggcgtcgc ccgcggcctg gtcgagcgcg
     6421 aactcgggcg aagcatcgcg gatgcctacg ccagcttcga caccaccccg ctggcatccg
     6481 ccagcatcgc gcaggtgcac gccgctaccc tgcacgacgg ccgcgaggtt gtggtcaagg
     6541 tgctgcggcc gcggatcgag gtgcagatcg ccgccgacat cgccctgctc aagagcgtgg
     6601 cgggcatcgt cgagcgcgcg catccgaatg ccgacaagat ccgcccgcgc gagatcgtgg
     6661 ccgagatcga gaacaccctg gccgccgaac tcgacctcca gcgcgaagcc ggcaatgcct
     6721 cgctgctgcg ccgcaactgg gagaattcgc ccgacctgta cgtgccggag gtgatctggt
     6781 cgcacaccgc cgagggcgcg atgacgatgg agcgggtgcg cggcatcccg tcggacgaca
     6841 tcaaggcgct ggatgccgcc gggatcgacc gccgtcggct tgcggccaag ggcgtgcgcg
     6901 tgttctacca gcaggtgttc cgcgacaact tcttccacgc cgacgcgcac gccggcaaca
     6961 tctgggtgga tgcgacgcgc aagcaggacc cgcgcttcat cgcgctggat ttcgggatca
     7021 tgggccagct gtc
//
LOCUS       DAORUK010000110         6904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_12592_length_6904_cov_15.760403, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000110 DAORUK010000000
VERSION     DAORUK010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6904
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12592_length_6904_cov_15.760403"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PK818_07895"
     CDS             complement(<1..543)
                     /locus_tag="PK818_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82687.1"
                     /translation="MNLLAAAAVLLVLAAVALALLLRSRNMHIWIVDYLRRPARPPVD
                     GPTHVMFCFVDHFEPMWGKADADTQHRRVERWCRDYRALASRHRDADGRPPQHTFFYP
                     EEEYVPELLDQLAALCGEGYGEIEVHLHHDNDTEANFRTSVARFRDLLHARHGALPRD
                     PASGELRFGFIHGNWCLDNSR"
     gene            complement(540..1133)
                     /locus_tag="PK818_07900"
     CDS             complement(540..1133)
                     /locus_tag="PK818_07900"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQY82688.1"
                     /translation="MKSRGSARDAAIYLTFDDGPHPEHTPRVLDLLARHGARGSFFVI
                     GDKVRANPDLARRIAAEGHLLGNHSNTHPEFRALDLAQQLRELELADQALAAVGGGSE
                     PLVRTPRGVLPPRLVLELARRGRPIVYWSYDTMDYRRGPPAEVIDRLRREPPQPGDIL
                     LMHDDGPAVIGILEAMLPEWTARGLLLRALPAYGEAA"
     gene            1489..2751
                     /locus_tag="PK818_07905"
     CDS             1489..2751
                     /locus_tag="PK818_07905"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQY82689.1"
                     /translation="MFLFVLLYLALVIVRPQEYPALVDWGVPVLQITLGGAFLLWILG
                     ARKDFGAPQFPLLFAFLLVLMLSLAVNGWLGGTLDQLTRFGPVVAAFVLVAHAITSHR
                     RARIVMATLVLCTLVPAIHGIDQKAKGIGWTGVGLIQEDGRIQYLGIFNDPNDLGMLF
                     VIALPMALLLSRGGGLMGLRRLFWLAAAGTILYAIYLTNSRGALLATAALVVIQLWRR
                     RGFVVASLVGVFGLAGLMMLPSRLSQLDVEEASAYGRVDAWYEGLQMFAANPVFGIGA
                     NQFSDFHHLTAHNSFVLVLAETGITGFTIWVAFLAYCFRMIFAVLRSPVAAAPGGITE
                     DDRSFAATLLLSLSGCMVAAFFLSRTYIVLIYLLTGLIVGFYLEARRRAPALPRFPLS
                     NDLLLWPFVGVAGAVGLYIVVKVLLMTV"
     gene            2748..4301
                     /locus_tag="PK818_07910"
     CDS             2748..4301
                     /locus_tag="PK818_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQY82690.1"
                     /translation="MSTRTTTLRNTAFTSVAIYTEYFLGMLTSILIARNLGPDGFGAY
                     SLIIWLVAVSMTVVNAGTAGAVIRFVAELRGAGTPELIVPLLAFLRRVQQWFLLVVLA
                     GGAALFWFAGDHLVPGFNHLLLFLLMALSTALRANYMFNVCIAKGFEHFRATAVIATV
                     AAPANLLLVALAWWVSAPVEGFLAVYAVSSAVFYFVSSRQTRGLLPDAAAGLALPGSV
                     HARVVRHMRITAAMVIITYVTASEVEVMFLNLLDTAAAAGKFKVAYQLADGATLLVPG
                     VFGVLLLPMMANALSQSREAAGRRFVGATTYLALLAMPLLAFGILFAEPVMAVLYGNA
                     YLAAAPAFAVILLAKSVSVVSQGGSSLLMSSERQNVVLVIVILCGLLKIALDVVLIRR
                     YGLHGAIVAYAVATIASAAAMIVFGARTGGTGLPWGRLLRIGGAGAIAALCALPLHWL
                     GHPLPAVVAGFLVISLSYAFATLLLGCWSEADIAHIRGMHARLASGRPRAVDKLLQWA
                     GRRAAREAT"
     gene            4298..5656
                     /locus_tag="PK818_07915"
     CDS             4298..5656
                     /locus_tag="PK818_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HQY82691.1"
                     /translation="MSDWYETLFRGVLFPLYESGLRRRHTVRYLREYERNQWLPPERI
                     AAIQWEKLQKLLAHCWNEVPYYRRRWSEAGIEPGDIRTLEDFARLPLLTKQDIRQNYE
                     DIHARSLRGQMLYKSTSGSTGEPLRLGYTRESFERRIAVMWRGYGWAGSRMGRRTLYL
                     WGAPVDGPDDLHRVKDRIYHRVFGRKVLNTYLMSDGNMAAYADEIDRYRPRVIVGYVN
                     PVVRLAQWLLEHGRKVHRPLAILGAAEALHDFQREIIEEAFGCKAYNTYGCREFMLVA
                     SECERQDGLHVNADHLVVEVADRKPALDGSEIGEVVVTDLHNYGMPFVRYANGDTAVP
                     GPATPCACGRGLPRLARVVGRTLDSIRAPDGRVVPGEFFPFVFNDIPGVLRFRVHQRQ
                     LDRLDISLVTDAAYDAASERRCREQIGRVFGERMTIAIERVDELPLGPSGKARVTVSE
                     IE"
     gene            5653..6783
                     /locus_tag="PK818_07920"
     CDS             5653..6783
                     /locus_tag="PK818_07920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY82692.1"
                     /translation="MSDAPTRLTHVVENLNRGGLERVVIDLVRAQRELGYDCEVVCLF
                     EAGSLAAEVTAAGIPVHACGKRGGLDLGAIARLRGHLRRHRTQVLHTHNAVAHYHAVA
                     AALGLGIRRTVNTRHGMGVQQAGGRREWLFRRTLGRTDVVATVCEAARRDVLAQGLIP
                     AAKVVAIPNGIRLDAFEPASDAARASLVRELGLPQGTRLIGAVGRLNWAKDPVSLVQA
                     FRAVRERHPATALVWIGDGALRTEVEAAATAEGVADRVILLGDRSDVPRLLTGLDLYA
                     MSSLTEGYSIALLEACAAGLPIVATDVGGNGEIVRDGVNGLLVPASDPQRVAGALNIL
                     LDDAGRCQQMGQAGREWSLVAATMVAMAKRYAAAYRGAGGAK"
BASE COUNT          906 a         2414 c         2454 g         1130 t
ORIGIN      
        1 gcgcgagttg tcgaggcacc agttgccgtg gatgaagccg aagcgcagct cgccgctggc
       61 cgggtcgcgc ggcaacgcgc catggcgcgc atgcagcagg tcgcggaagc gcgcgaccga
      121 ggtgcggaag ttggcctcgg tgtcgttgtc gtggtgcagg tgcacctcga tctcgccgta
      181 gccctcgccg cacagcgctg cgagctggtc gagcaattcg ggcacgtact cctcttccgg
      241 gtagaagaag gtgtgctgcg gcgggcgccc gtcggcgtcg cggtggcgcg aagccagggc
      301 gcggtagtcg cggcaccagc gctcgacgcg gcggtgctgg gtgtcggcgt cggccttgcc
      361 ccacatcggc tcgaagtggt cgacgaagca gaacatcacg tgcgtgggcc cgtccaccgg
      421 cggccgcgcc ggccgccgca ggtagtccac gatccagatg tgcatgttgc gcgagcgcag
      481 cagcagcgcc agcgcgaccg ccgccagcac cagcagcacg gccgccgcgg ccagcaggtt
      541 cacgcggcct ccccgtaggc gggcagggcg cgcagcagga ggccgcgcgc cgtccactcg
      601 ggcagcatcg cttccaggat cccgatcacc gccggaccgt cgtcgtgcat cagcaggatg
      661 tccccgggct gcggcggctc gcgccgcagc cggtcgatga cctcggcggg cggtccgcgc
      721 cggtagtcca tggtgtcgta ggaccagtac acgatggggc gaccgcgccg cgccagttcg
      781 agcaccaggc gtggcggcag gacgccgcgc ggcgtgcgca ccagcggctc gctgccaccg
      841 ccgaccgcgg caagcgcctg gtcggccagt tccagttcgc gcaactgctg ggccaggtcc
      901 aacgcgcgga attccgggtg ggtattcgag tggttgccga ggaggtggcc ctcggccgcg
      961 atgcggcgcg ccaggtccgg gttcgcccgc accttgtcgc cgatgacgaa gaaactgccg
     1021 cgggcgccgt ggcgcgcgag caggtcgagc acgcgcggcg tgtgctccgg gtgcgggccg
     1081 tcatcgaacg tgaggtagat cgcggcgtcg cgggccgaac cacgcgattt cacgatcccg
     1141 cgagggatcc agcgcagcag ctgcaatttc ctgggccgag gtcccatggt cctttttccg
     1201 tgccgtcgcg gatgtcggca gcgccgccgc ccggcccatg ggcgtggcca ttgcgcgagc
     1261 gggaggcacc gcggtttgcg accgcggcat tatgaccggc gaaaacgaca atcgcaccca
     1321 ccgggcgccc gggggcgggc agcggtcgct gccgtgaccc cgggggctgg tggtaccttt
     1381 gtcgccgctc gtgccgccgg gcgagggtcc gcgatggcgg gccctgcggc acggcgcggc
     1441 cttccagccc ccgtcccgtc cacgctgcca ccggcgaccg caacctgcat gttcctgttc
     1501 gtgctgctct atctcgcact cgtcatcgtg cggccgcagg agtacccggc gctggtggac
     1561 tggggcgtgc cggtgctgca gatcacgctc ggcggcgcct tcctgctctg gatcctcggc
     1621 gccaggaagg acttcggcgc gccgcagttc ccgctgctgt tcgcgttcct gttggtgctg
     1681 atgctctccc tggccgtcaa cggctggctg ggcggcacgc tcgaccagct gacgcgcttt
     1741 ggtcccgtgg tcgcggcctt cgtgctggtg gcccatgcca tcacctccca ccggcgcgca
     1801 cggatcgtga tggcgacgct ggtgctgtgc accctggtgc cggcgatcca tggcatcgac
     1861 cagaaggcca agggcatcgg ctggacgggc gtcggcctga tccaggagga cgggcgcatc
     1921 cagtacctgg gcatcttcaa cgaccccaac gacctcggca tgctgttcgt gatcgcgctg
     1981 ccgatggcct tgctgctgag ccggggcggc ggcttgatgg ggctgcggcg cctgttctgg
     2041 ctcgccgccg caggcacgat cctgtacgcc atctacctga ccaattcgcg cggcgcgctg
     2101 ctcgccaccg cggcgctggt ggtgatccag ctgtggcggc ggcgcggctt cgtggtcgca
     2161 tcgctggtcg gcgtgttcgg actggcgggg ttgatgatgc tgccgtcgcg gctgagccag
     2221 ctcgatgtcg aggaggcatc ggcgtacggg cgggtcgatg cctggtacga gggcctgcag
     2281 atgttcgcgg ccaatccggt cttcggcatc ggcgccaacc agttctccga cttccaccac
     2341 ctgaccgcgc acaactcctt cgtgctggtg ctggccgaga ccggcatcac cggcttcacg
     2401 atctgggtcg cgttcctggc ctactgcttc aggatgatct tcgccgtcct gcgcagcccg
     2461 gtcgcggccg cgccgggcgg gatcaccgag gacgaccgat cgttcgccgc gaccctgctg
     2521 ctgtcgctga gcggctgcat ggtcgcggcc ttcttcctca gccgcaccta catcgtgctg
     2581 atctacctgt tgaccggatt gatcgtgggc ttctacctcg aggcgcgccg gcgcgcgccg
     2641 gccttgcccc gcttcccgct ttcgaacgac ctgctgctat ggcccttcgt cggcgtcgct
     2701 ggcgcagtcg ggctgtatat cgtggtcaag gtcctgttga tgaccgtatg agcacccgga
     2761 cgaccacgct gcgcaacacg gccttcacct cggtggcgat ctacaccgag tacttcctcg
     2821 gcatgctgac ctcgatcctg atcgcccgca acctcgggcc cgacggcttc ggtgcgtaca
     2881 gcctgatcat ctggctggtc gcggtcagca tgaccgtggt caatgccggc accgccggcg
     2941 cggtgatccg cttcgtggcc gagctgcgcg gcgccgggac cccggaactg atcgtgccgc
     3001 tgctggcgtt cctgcggcgc gtgcagcagt ggttcctgct ggtggtgctg gcgggcggcg
     3061 ccgcgctgtt ctggttcgcc ggcgaccacc tcgtgccggg cttcaaccac ctgctgctgt
     3121 tcctgctcat ggcgctctcc accgcgctgc gcgccaacta catgttcaac gtgtgcatcg
     3181 ccaagggctt cgagcatttc cgcgccaccg cggtgatcgc cacggtggcg gcgccggcca
     3241 acctgctgct ggtggcgctg gcatggtggg tcagcgcgcc ggtcgagggc ttcctggcgg
     3301 tctatgcggt ctcgagcgcc gtgttctatt tcgtctcgag ccggcagacc cgcggcctgc
     3361 tgccggacgc cgcggccggg ctggcgctgc ccggatcggt ccatgcgcgc gtggtgcgcc
     3421 acatgcgcat caccgcggcg atggtcatca tcacctacgt gaccgcgagc gaggtcgagg
     3481 tgatgttcct caacctgctc gataccgcgg ccgcggccgg caagttcaag gtcgcctacc
     3541 agttggccga tggcgccacc ctgctggtgc cgggcgtgtt cggcgtgctc ctcctgccga
     3601 tgatggccaa tgccctgagc cagagccgcg aggccgccgg ccgccgcttc gttggcgcga
     3661 ccacctacct ggcgctgctg gcgatgccgc tgctggcctt cggcatcctg ttcgcggagc
     3721 cggtgatggc ggtgttgtac ggcaacgcct atctcgccgc cgcgccggcc ttcgcggtga
     3781 tcctgctggc gaaatcggtg tcggtggtct cgcagggggg ctcgagcctg ttgatgagct
     3841 ccgagcgcca gaacgtcgtg ctggtgatcg tcatcctctg cggcctgctg aagatcgcgc
     3901 tggacgtggt cctgatccgc cggtacggcc tgcacggggc gatcgtcgcc tacgcggtgg
     3961 ccaccatcgc gagcgccgcg gcgatgatcg tgttcggtgc gcgcaccggc ggcaccggcc
     4021 tgccctgggg acgcctgctg cgcatcggtg gcgccggcgc gatcgcggcc ctgtgcgcat
     4081 tgccactgca ttggctcggg cacccgctgc cggccgtggt tgcgggtttc ctggtcattt
     4141 cgctgtccta cgccttcgcg accctactcc tgggttgctg gagcgaggcc gacatcgcgc
     4201 acatccgcgg catgcacgca cgcctggcct cgggccggcc gcgtgcggtc gacaaactgc
     4261 tgcaatgggc gggccgccgc gcggcccggg aggcgacgtg agcgactggt acgaaaccct
     4321 gttccgcggc gtgctgttcc cgctgtacga aagcgggctg cggcgccgcc ataccgtgcg
     4381 ctacctgcgc gagtacgagc gcaaccagtg gctgccgccg gagcgcatcg ccgcgatcca
     4441 gtgggagaag ctgcagaagc tgctcgcgca ttgctggaac gaggtcccct actaccggcg
     4501 ccgctggtcc gaggccggca tcgagcccgg cgacatccgc acgctggagg acttcgcgcg
     4561 cctgccgctg ctgaccaagc aggacatccg ccagaactac gaggacatcc acgcgcgatc
     4621 cctgcgcggc cagatgctct acaagagcac cagcggctcg accggcgagc cgttgcgcct
     4681 gggctacacg cgcgagagct tcgagcgccg catcgcggtg atgtggcgcg gctacggctg
     4741 ggccggatcg cgcatgggcc gccgcacgct gtacctgtgg ggcgcaccgg tggacgggcc
     4801 cgacgacctg caccgggtca aggaccgcat ctaccaccgc gtgttcggcc gcaaggtgct
     4861 caacacctac ctgatgtcgg acggcaacat ggcggcatac gccgacgaga tcgaccgcta
     4921 ccgtccgcgg gtgatcgtcg gctacgtcaa cccggtcgtg cggctggcgc aatggctgct
     4981 cgagcacggg cgcaaggtgc atcgcccgct ggcgatcctc ggcgcggccg aagcgctgca
     5041 cgatttccag cgcgagatca tcgaggaggc cttcggctgc aaggcctaca acacctatgg
     5101 ctgccgcgag ttcatgctgg tcgcctcgga gtgcgagcgc caggatggcc tccacgtcaa
     5161 cgccgatcat ctggtggtcg aagtcgccga tcgcaagccg gccctggacg gcagcgagat
     5221 cggcgaagtg gtggtgaccg acctgcacaa ctacggcatg cccttcgttc gctacgccaa
     5281 cggcgacacc gcggtgccgg gcccggcgac gccctgcgcc tgcggccgcg ggctgccgcg
     5341 gctggcgcgc gtggtcggcc gcacgctgga ctcgatccgg gcgcccgatg gccgggtcgt
     5401 gccgggtgag ttcttcccgt tcgtgttcaa cgacatccct ggcgtcctgc gtttccgcgt
     5461 gcaccagcgc cagctcgacc gcctcgacat cagcctggtg accgatgccg cgtacgacgc
     5521 ggcgtccgag cggcgctgcc gcgagcagat cggacgcgtg ttcggcgagc gcatgaccat
     5581 cgcgatcgaa cgcgtggacg aattgccgct cggaccctcc ggcaaggccc gggtcacggt
     5641 gtcggaaatc gaatgagcga cgcgccgacg cgcctgaccc acgtcgtcga gaacctcaat
     5701 cgcggcgggc tcgagcgcgt ggtcatcgac ctggtgcgcg cgcagcgcga gctgggttac
     5761 gactgcgagg tcgtgtgcct gttcgaggcc ggctccctcg ctgcggaagt gacggcggcg
     5821 ggcatcccgg tccatgcctg cggcaagcgc ggcggcctgg acttgggcgc catcgcccgc
     5881 ctccgtggcc acctgcgccg ccaccgcacg caggtgttgc acacccacaa tgccgtcgcg
     5941 cactaccacg cggtcgcggc agccctgggc ctgggcatcc ggcgcacggt caacacccgc
     6001 catggcatgg gcgtgcagca ggccggagga cggcgcgagt ggctgttccg gcgcaccctg
     6061 gggcgcaccg acgtggtggc caccgtgtgc gaggcggcgc gccgggacgt gctggcccag
     6121 ggcctgatcc ccgcggccaa ggtggtcgcg attcccaacg gcatccgcct cgatgccttc
     6181 gagcccgcca gcgacgcggc gcgcgcgtcg ctggtgcgcg agctgggcct gccgcaaggc
     6241 acccggctca tcggcgccgt ggggcgcctc aactgggcca aggatccggt gtcgctggtg
     6301 caggcctttc gcgcggtgcg cgagcgccac ccggcgaccg cgctggtctg gatcggcgat
     6361 ggcgccctgc gcaccgaggt ggaggccgcc gccacggcag agggggtcgc cgaccgcgtg
     6421 atcctgctcg gcgaccgcag cgacgtgccg cgcctgctca cgggactgga tctctacgcc
     6481 atgtcctcgc tgaccgaggg ctactcgatc gcgctgctcg aagcctgcgc cgccggcctg
     6541 cccatcgtcg ccaccgacgt cggcggcaac ggcgagatcg tgcgcgacgg ggtcaatggc
     6601 ctgctggtgc ctgcaagcga cccgcaacgc gtcgctggag cgctcaacat cctgctcgac
     6661 gacgctgggc gttgccaaca gatgggccaa gccggtcgcg agtggtccct ggtcgcagcc
     6721 acgatggtgg cgatggcgaa gcgctatgcc gcggcatacc ggggcgctgg cggcgcgaag
     6781 taggggcggt cgcggggacc gctacggccg cggcttcccg tggcagcgct acgccggatt
     6841 ccggtccgag gcccgcgcca cgaagttgac cgaggtcgcg accaggtcct cggcggcgta
     6901 cgag
//
LOCUS       DAORUK010000111         6894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_12624_length_6894_cov_17.549642, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000111 DAORUK010000000
VERSION     DAORUK010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12624_length_6894_cov_17.549642"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PK818_07925"
     CDS             complement(<1..284)
                     /locus_tag="PK818_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82693.1"
                     /translation="MSINKILLAMALGLALTACSNQEPAADAAAPAADAAAADAAAAP
                     ADAAATDAQAAADAAAAAAAADAAAPAADAAAAPADAAAPADAAAAPADAA"
     gene            complement(415..1590)
                     /locus_tag="PK818_07930"
     CDS             complement(415..1590)
                     /locus_tag="PK818_07930"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005911622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HQY82694.1"
                     /translation="MSDIVIVGAKRTAIGSFLGQFTGTPTTALGTAAISAALAHSGLA
                     ADQVDEVIMGCVLPAGLGQAPARQAARGAGIPDAAGCTTINKVCGSGMKAVMLAHDII
                     KAGSARAVVAGGMESMTNAPHLLNGSRTGIRYGSAEMLDHMAWDGLTNPYDGKAMGVF
                     GDAACAKYGYDRATVDAFSAESARRAQAAQANGAFAGEIAPVTVKTRKGEVVVDRDEE
                     PGKIDLGKIPELRAAFGKEGVLTAASSSKISDGAAALVVMSAEAAAAAGLQPLARIVA
                     HATHAQAPEWFTTAPVAAISNVLAKAGWTVADVDLFEVNEAFACVAMAPMTDLGIPHD
                     RLNVNGGALALGHPIGASGARLVVTLLNALEARGGKRGVASLCIGGGEATALAVELP"
     gene            1816..4746
                     /locus_tag="PK818_07935"
     CDS             1816..4746
                     /locus_tag="PK818_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQY82695.1"
                     /translation="MQIRPGRRLASALLPLSIALALAACGGGGGGNVRPTPPPPTTPT
                     GPGFTPTVDNDSSLTTLNPPAIPAQGVPVSLTDASINRHLSLTNALGALGAGLKGQGV
                     TIGFVDSGVNRSHPTLTGRVTQSFIHVSSPPNDLSVDDKVGHGTVVASLAAGRPAPGL
                     YSGGETGNWGGGIAQSASVVSSRIIGDARPDDDGSGEGNEIGAGQGYGEFFQAINAEL
                     ANAGAKVINNSWGGLYWNDPALSIELANAWKDFVVGRGGIVVFANGNSGEDPRFVGNP
                     SDNAALPSIANDPVLERGWLTVGALDPANPTQLTSYSQQCGIAMNYCLVAPGNVVFID
                     ADATSAATSKLYQGGGTSYAAPLVSGAAAVVWSAFPYFSNDQVRQAILAGARDLGAAG
                     VDPVFGWGLLDVTKAANGPSNFAWGDFSVSFSGHSVWRNPIIGSGGLVKGGSGTLTLA
                     EAGNFTGATRVDAGGLDVRKGLRSNLGIANGATVWASGAFGGNVNNDGRFFNGASVPA
                     TIAGNFIQSSTGNLGIWLGSTLRVTGSATLNGQMSILGVRSGYTTTAKETLLNASAGV
                     SGTFTTLKAAPNVFLDANLAYDPNNVFLNINRINVANAVAGMGLSTITSVSAQRVEAA
                     MQAIDGQLAGSLPVGIGSAFIDAAGALQQSTSIANADLSLRSLSGELHAASTAMTFDA
                     IDVGRRALAGRLDTLSHAPQAVGGWYRNLASGGTLAQAGFDGVGIDSAGEMIGNDWRI
                     GSDAVVGIAMNRLEQSSWLGEFGDRSRGRQRELQLYASSWRGDSYAQAQFASGSFQRQ
                     MQRNLLLGSLQDAVATQLSGDYLGASGEIGQRFRAGGVALTPYLGAQYVQVANDGFSE
                     GGASGFGLRADAWDSGRLQGFAGLRAARGWRIGEVDLLADARAEWQHTLASRGELFDA
                     SFTGLEQWAPLQGIGMARRSRLFGVGLSAMFAGKAMFRFDLGRRASDVGGGNLASLQA
                     MYRF"
     gene            complement(4817..5974)
                     /locus_tag="PK818_07940"
     CDS             complement(4817..5974)
                     /locus_tag="PK818_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HQY82696.1"
                     /translation="MRPFPLGEDIDALRDAVRRFAEAEIAPRAAQVDEDNWFPQDLWP
                     KLGEMGLLGMTVPAEHGGSGMGYLAHLVAMEEISRASGSIGLSYGAHSNLCVNNLYAN
                     GNEAQRAKYLPRLCSGEWKGALAMSEPGAGSDVVGSMACRAELRGGMWIANGNKMWIT
                     NGPEADVLVVYMRTAGKEAGSKCMTAFIVEKGMPGFSTAQKLDKLGMRGSNTCELVFE
                     DCAIPPENVLGEVNQGVKVLMSGLNTERLVLTGGPLGLMQAALDITLPYVRDRRQFDA
                     AIGTFGIMQAKVADMYTALQSARAFGYQVARDYDSGYKSRVDAASCLLHASDAAVQVA
                     LEAIQSLGGNGYINEYPTGRILRDAKLYAIGAGTNEIRRMLIGRELFAGNS"
     gene            complement(6122..>6894)
                     /locus_tag="PK818_07945"
     CDS             complement(6122..>6894)
                     /locus_tag="PK818_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase family protein"
                     /protein_id="HQY82697.1"
                     /translation="TAEDVGIDVNDMEYVYRETEYVTGVHQVHGGSGDPSPFTAYGTL
                     QGLMATLNQKYGDEEVGKYSYAVQGLGHVGMEFVKLLKERGAKIFVTDINQGLVDKAV
                     SEYGAEAVGLDEIYDVAADVYSPCALGGTINEQTLPRLKAKVICGAANNQLANNAIGD
                     EVTKRGILYAPDYAVNAGGVMNVALELDGYNRERAMRMMRTIYHNLHRIYEISSRDGI
                     PTYMAADRLAEERISSIGKLKLPLGRGAPRFQGRIRGH"
BASE COUNT         1023 a         2305 c         2417 g         1149 t
ORIGIN      
        1 gcggcgtcgg ccggggcagc ggcggcgtcg gccggggcgg cggcgtcggc cggggcagcg
       61 gcggcgtcgg cggccggagc ggcggcgtcg gcagcggcag cggcagcggc agcgtcggcg
      121 gcagcctgcg cgtcggtggc ggcggcgtcg gccggggcag cggcggcgtc ggcagcggcg
      181 gcgtcagcgg ccggggcagc ggcgtcggcg gccggttcct ggttgctgca cgcggtcagg
      241 gccaggccca gcgccatcgc cagcaggatc ttgttgatcg acatgatgcg tattcctcgt
      301 ctatgaatag ggtattggcg gaacgcgcat actgcgcgcc ggtaacatga tgacaagcca
      361 ttgtcgcgtg tcaagcggtt ggccgacttt ttttcatgta accgtttaag catctcacgg
      421 aagttcgact gccagcgcgg tcgcttcgcc gccgccaatg cacagggacg ccaccccgcg
      481 cttgcctccg cgggcctcca gggcgttcag cagggtgacg accaggcgcg ccccggacgc
      541 accgatcggg tggcccaggg cgagggcgcc gccgttcacg ttcagcctgt cgtgcgggat
      601 gccgaggtcg gtcatcggcg ccattgccac gcaggcgaac gcttcgttga cctcgaacag
      661 gtccacgtcc gccaccgtcc agccggcctt ggccagcacg ttggaaatgg cggcgaccgg
      721 tgcggtggtg aaccattccg gcgcctgcgc gtgggtggcg tgggccacga tccgcgccaa
      781 cggctgcaat ccggcggctg cggcggcctc ggccgacatc accaccagcg cggcggcgcc
      841 atcggaaatc ttggacgagg aggccgcggt cagcacgcct tccttgccga aggcggcgcg
      901 caattccggg atcttgccga ggtcgatctt gcccggctct tcatcccggt cgaccacgac
      961 ttcgcccttg cgggtcttga ccgtcaccgg ggcgatctcg ccggcgaacg caccgttggc
     1021 ctgcgcggcc tgcgcgcgcc tggcgctctc ggcggaaaac gcgtccaccg tggcgcggtc
     1081 gtagccgtat ttggcgcagg cggcatcgcc gaacacgccc atcgccttgc cgtcgtaggg
     1141 attggtgagg ccgtcccacg ccatgtggtc cagcatctcc gcggatccat agcgaatgcc
     1201 ggtgcgcgaa ccgttcaaca ggtgcggggc gttggtcatc gattccatgc cgccggccac
     1261 caccgccctt gcggaaccgg ccttgatgat gtcgtgcgcc agcatcaccg ccttcatgcc
     1321 ggagccgcag accttgttga tggtggtgca gccggcggcg tccgggatac cggcgccgcg
     1381 cgctgcctgc cgcgccggcg cctggccgag gccggccggc agcacgcagc ccatgatcac
     1441 ttcgtcgacc tggtcggcgg cgaggccgga gtgggcgagc gccgcggaaa tcgcagcggt
     1501 accgagggcc gtggtgggcg taccggtgaa ctggccgagg aaggaaccga tggcggtgcg
     1561 cttggcaccg acgatgacga tgtcgctcat ggggggctcc gggaaagtca gggggcgagt
     1621 ggccgcgggc agattcgcgc gtccgattat ggcgcgaaat ggcgggcgtt gctgcgctgc
     1681 ggcatcggcc gttcctgttc aggatgaatc cgttgtgacg cgggcccgct tggcatacaa
     1741 catgcaaatt ccgggaacga ggcggaccgg ggccgccggt gcgaagccaa cgagaccatc
     1801 atgacgattg catccatgca gattcgaccc gggcgccggc tggcgtccgc cctgttgccg
     1861 ctttcgatcg cgctggccct ggcggcatgc ggtggaggtg gcggcggcaa cgtgcgtccg
     1921 accccgccgc caccgaccac gccgaccggc cccgggttca cgccgacggt tgacaatgac
     1981 agctcgctga ccacgctgaa tccaccggcg atcccggccc agggcgttcc ggtatcgctg
     2041 accgacgcca gcatcaaccg ccacctcagc ctgaccaatg cgctgggggc gctgggtgcc
     2101 ggcctgaagg gacagggcgt caccatcgga ttcgtggatt ccggcgtgaa ccgcagccac
     2161 ccgacgctga ccggccgggt cacccagagc ttcatccatg tgtcctcgcc gccgaacgac
     2221 ctgtcggtgg acgacaaggt cgggcacggc accgtggtgg cctcgctggc tgcggggcgg
     2281 cccgcacccg ggctctacag tggtggcgag accggcaact ggggcggcgg gatcgcgcag
     2341 agcgcaagcg tggtgtcgtc gcgcatcatc ggcgatgcgc gacccgatga tgatgggtcg
     2401 ggggaaggta acgaaatcgg tgcgggccag ggctacggcg aattcttcca ggccatcaat
     2461 gccgaactgg ccaacgccgg cgcgaaggtc atcaacaatt cgtggggcgg cctgtactgg
     2521 aacgatcccg cgctgagcat cgagctcgcc aatgcctgga aggacttcgt ggtgggacgc
     2581 ggcggcatcg tcgtgttcgc caatggcaac agcggggaag atccgcgctt cgtgggcaat
     2641 ccctcggaca atgccgcgct gcccagcatt gccaacgacc cggtgctcga gcggggctgg
     2701 ctcaccgtgg gtgcgctgga cccggccaac ccgacccagc tgaccagcta ttcgcaacag
     2761 tgcggcatcg cgatgaacta ttgcctggtg gcgcctggca acgtggtgtt catcgatgcg
     2821 gacgcgactt ctgccgctac cagcaagctc taccagggcg gcggcacctc atacgcggcg
     2881 ccgctcgtct ctggcgctgc cgcggtggtg tggtcggcgt tcccgtactt cagcaacgac
     2941 caggtgcggc aggcgatcct cgctggagcc agggatctcg gtgccgccgg ggtcgatccg
     3001 gtgttcggtt ggggcctgct ggacgtgacc aaggcggcca acggtcccag caatttcgcc
     3061 tggggcgact tcagcgtttc gttcagcggc cactcggtct ggcgcaatcc gatcatcggc
     3121 agcggtggcc tggtcaaggg cggcagcggc accctgaccc tggcggaagc cggcaacttc
     3181 accggtgcca cccgcgtcga tgccggcggc ctggatgtcc gcaagggcct gcgctcgaac
     3241 ctgggcatcg ccaacggcgc gacggtctgg gccagcggcg ccttcggcgg caacgtgaac
     3301 aatgacgggc gcttcttcaa cggcgccagc gtgccggcca ccatcgccgg caatttcatc
     3361 cagtcgtcga ccggcaacct tggcatctgg ctgggcagca ccttgcgggt gaccggcagc
     3421 gcgacgttga acgggcagat gtcgatcctc ggcgtccgca gcggctacac caccaccgcc
     3481 aaggaaaccc tgctcaacgc cagcgccgga gtcagcggca cgttcacgac gctgaaggcc
     3541 gcgccgaacg tgttcctcga tgccaacctg gcgtatgacc cgaacaacgt cttcctcaac
     3601 atcaaccgca tcaacgttgc caatgcggtc gccggcatgg gcctgtccac catcaccagc
     3661 gtctccgccc agcgcgtgga agcggcgatg caggcgatcg acgggcaact ggccggcagc
     3721 cttccggtgg gcatcggcag tgccttcatc gatgccgccg gggcgttgca acagtcgacc
     3781 agcatcgcca atgccgacct gtcgctgcgt agcctgtcgg gcgaattgca cgcggcatcg
     3841 acggcgatga ccttcgatgc catcgacgtc ggccggcgtg cgctggcggg ccggctcgac
     3901 accctctcgc atgcgccgca ggccgttggc ggctggtatc gcaacctcgc cagcggcggc
     3961 accctggcgc aggccgggtt cgacggcgtc ggcatcgaca gcgccggcga aatgatcggc
     4021 aacgactggc ggatcggcag cgatgcagtc gtcggcatcg cgatgaaccg gctcgagcaa
     4081 tccagctggc tgggcgaatt cggcgaccgc agccgtggcc gccagcgcga gctgcagctc
     4141 tacgcgtcgt catggcgcgg tgattcgtat gcgcaggcgc agttcgcgtc cggcagcttc
     4201 cagcggcaga tgcagcgcaa cctgctgctg ggctccctgc aggacgcggt ggcgacgcaa
     4261 ctgtccggcg attacctcgg tgcatcgggc gagatcggcc agcgcttccg ggcgggcggg
     4321 gtcgcgctga ccccttacct gggcgcgcag tacgtgcagg tcgccaacga tggcttcagc
     4381 gaaggcggcg cgagcggctt cggcctgcgt gccgatgcgt gggacagcgg gcgcctgcag
     4441 ggcttcgccg gcctgcgtgc cgcgcgcggc tggcgcatcg gcgaggtcga cctgctcgcg
     4501 gatgcgcgcg ccgaatggca gcacacgctg gcgtcgcggg gcgagctgtt cgatgccagc
     4561 ttcaccggac tggagcaatg ggcgccgttg cagggtatcg ggatggcgcg gcgcagccgg
     4621 ttgttcgggg ttggcctgtc ggcgatgttc gccggcaagg cgatgttccg cttcgacctg
     4681 gggcggcgtg ccagcgacgt cggtggcggc aacctggcct cgctgcaggc gatgtaccgc
     4741 ttctgaagcg atggccgtgc aaacgggaaa cgccgcagcg atgcggcgtt tttcgttgca
     4801 cgggtgcaac cgaaggtcag ctgttgccgg cgaacagctc gcgcccgatc agcatgcgcc
     4861 ggatctcgtt ggtgccggcg ccgatcgcgt acagcttggc atcgcgcagg atgcggccgg
     4921 tcggatattc gttgatgtag ccgttgccgc caagcgactg gatcgcttcc agcgcgacct
     4981 gcaccgcggc gtcgctggca tgcagcaggc acgatgcggc gtcgacccgg ctcttgtagc
     5041 cgctgtcgta gtcgcgcgcg acctggtagc cgaacgcgcg cgccgactgc agcgcggtgt
     5101 acatgtcggc gaccttcgcc tgcatgatcc cgaaggtgcc gatcgcggcg tcgaactgcc
     5161 ggcggtcgcg cacgtacggc agggtgatgt cgagcgcggc ctgcatcagg cccagcgggc
     5221 cgccggtcag caccaggcgc tcggtgttga ggccggacat cagcaccttg acgccctggt
     5281 tgacctcgcc gaggacgttc tccggcggga tcgcgcagtc ctcgaacacc agttcgcagg
     5341 tgttggagcc gcgcatgccg agcttgtcca gcttctgcgc ggtggagaag cccggcatgc
     5401 ccttctcgac gatgaaggcg gtcatgcact tgctgcccgc ctccttgccg gcggtgcgca
     5461 tgtagacgac cagcacgtcg gcttccggac cgttggtgat ccacatcttg ttgccgttgg
     5521 cgatccacat gccgccgcgc agttcggcgc ggcaggccat cgagccgacc acgtcggaac
     5581 ccgcgcccgg ttcgctcatc gccagcgcgc ccttccactc gccgctgcac agccggggca
     5641 ggtacttcgc gcgctgcgcc tcgttgccat tggcgtacag gttgttcacg cacaggttcg
     5701 aatgtgcgcc gtagctgagg ccaatcgaac ctgacgcgcg cgagatctcc tccatcgcca
     5761 ccaggtgcgc gaggtagccc atcccgctgc cgccatgctc ggcgggcacg gtcatgccga
     5821 gcaggcccat ctcgccgagc ttcggccaca ggtcctgcgg gaaccagttg tcttcgtcta
     5881 cctgggccgc gcgcggggcg atctccgctt cggcgaagcg gcggaccgcg tcgcgcaggg
     5941 catcgatgtc ttcgccgagg gggaatggac gcatgggatg gttctcgcag ctggttggtt
     6001 gaagaccgca ggagcgggcc atgcccgcga ttgatccgat gacgcctcgg atcgcgccca
     6061 tggggcgctc ctacacggaa acggggcccg caggccccgt ccattatcgc ttatcgaccc
     6121 gtcagtggcc ccggatgcgg ccctggaagc gcggcgcacc gcggcccagc ggcagcttga
     6181 gcttgccgat cgagctgatg cgctcctcgg ccaggcggtc cgctgccatg taggtcggga
     6241 tgccgtcgcg gctggagatt tcgtagatgc ggtgcaggtt gtggtagatc gtgcgcatca
     6301 tccgcatcgc gcgctcgcgg ttgtagccgt ccagttccag cgccacgttc atcaccccgc
     6361 ccgcgttcac cgcgtagtcc ggcgcgtaca ggatgccgcg cttggtgact tcgtcgccga
     6421 tcgcgttgtt ggccagctgg ttgttggccg cgccgcagat caccttggcc ttcagccgcg
     6481 gcagggtctg ctcgttgatg gtgccgccca gcgcgcacgg cgagtacacg tccgccgcca
     6541 cgtcgtagat ctcgtccagg cccaccgctt cggcaccgta ctcggacacc gccttgtcga
     6601 ccaggccctg gttgatgtcg gtgacgaaga tcttggcgcc gcgttccttc agcagcttca
     6661 cgaattccat gccgacgtgg cccaggccct gcacggcgta ggaatacttg cccacttcct
     6721 cgtcgccata cttctggttg agggtggcca tcaggccctg cagggtgccg tacgcggtga
     6781 acggggaggg atcgccggag ccgccatgca cctggtgcac gccggtcacg tattcggttt
     6841 cgcggtacac gtattccatg tcgttgacgt cgatgccgac gtcctcggcg gtga
//
LOCUS       DAORUK010000112         6890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_12634_length_6890_cov_20.833943, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000112 DAORUK010000000
VERSION     DAORUK010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12634_length_6890_cov_20.833943"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PK818_07950"
     CDS             <1..608
                     /locus_tag="PK818_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY82698.1"
                     /translation="MQKHQYARWNQQAFDGVIGHYHVLPEQKVGQLSRGERAGFNLAL
                     ALAQRPELLVLDEPTLGLDVVAKRAFLESLLYSNAADDCTVIYCSHQMEEIERVADNL
                     IILERGQLKNMSAPEDFTARVSHWVADVPFKGPELHSVPGLLEVQRLDGLHHYLVLDQ
                     DAGFERFLRASGARNVQSMPVSLDRAVNAFLAKNHAAPAAA"
     gene            632..2440
                     /locus_tag="PK818_07955"
     CDS             632..2440
                     /locus_tag="PK818_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82699.1"
                     /translation="MIDLFKGELLRFRLWALVAAVINLVALGFMSRLVDLAQQPLVVY
                     QVASMVYAVAGALLGLFQMGSYRKPNQWLNLLHRPLHRLRIAGALTAAGVVVLLVAVA
                     LPIALVAVYQESLTPRVVDMRHWLLPIAAWLIAVSGYLAGAYAMVGDRRYAFTGFLLP
                     NLFMYSQASGVAMLGVHLFAIAFLVVLLAIAFKPDPAAPSRNPLAVLAVALPVQVGAY
                     FLAWMLGYGVELGWTVSGTHPLGMPVPPKGGYIEADRAEGKDMLLLGIDASRSPEAAL
                     WREQVALSDVHTRYPLRNLPVRNTLTNPAPMEFDDGENSVRWVFSHDRMRFVGYGTLD
                     KRARGELGVGETRAAFPAPTLQYAAGFLFNANAAYQYDAGQQRIFERIRLPRGEVMAS
                     PPEPAGDNVLVLSDRAAYFYPGREAVNGLDLLQPLLRVPMPGHVGNLSRVDAIELLDG
                     YLVSFTYTWGVWSGELQQPFQQLVRVDDAGKVHEVARRAIDVDLPSAYTNRNTWLSPV
                     IRALCLGARNLFAANDPLKAKPERVPPSVLWLAAACCLLSLLAAIWVSGRQVHSTRGR
                     WAWVVLCGVVGLPALASLWLLYPRREPLPVASPTLA"
     gene            2466..4535
                     /locus_tag="PK818_07960"
     CDS             2466..4535
                     /locus_tag="PK818_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQY82700.1"
                     /translation="MNISRIMCGALVACALLLSPRSVPAQVADGLQAAVAAQREMPRA
                     PRLPRAALLEGRTLVAVRLSPDGEHVAYLRERKDSRSLWLLPTTGGAARTLVGSTQAE
                     QLWWSRDGRWLFLRAPGTLTMVGVDGRSGVRVPLGGSGRRQVVKVDPSQPAAVLLRER
                     VRAGAGERWRIVRLDVHGKRTLLREDPRWIHDLALDARGRIVALARFEGDHDALHRVG
                     ADGRLREVLRLRPMQRIDLLATLADGSLLLEGNPGNGAEGNFRRVLRLGADDRLQTLH
                     QDPRGEADLDDVVLDPRTQLPVAASYRSTVAATHGIGSAQATVADIARRFPGRDLGLQ
                     VGSGPAARWLVSERAATLRDPRLHLYDPRNGSLRRILDEPGIGTKSPPEAALARKLAI
                     AYTASDGMRVHGFVLLPPGVDVARAPLVTQVHGGPINHFRAGFDGTAQFLANRGYVVF
                     QSNFRGSTGHGRDYVFAPKGDYGNGRVQQDIVEGVRWLLARGIGDAKRVGIVGHSFGG
                     YSTLLGLTFQPDLFKVGVAGAPPADLGWSMRWLVRSGSQGDLPDRSLRHTLRALSLDS
                     TDPATHARLHAQSPQANAAKMRRPLLIIAGGADRTVAIREVANYSATLKALGRPLTLL
                     VEPNGGHSPVDPVPREAYLFAMETMLQRHLGGPAPEQPGQRLRAYLRANLRLAGPEFT
                     TFAAPPR"
     gene            complement(4549..5748)
                     /locus_tag="PK818_07965"
     CDS             complement(4549..5748)
                     /locus_tag="PK818_07965"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQY82701.1"
                     /translation="MAVPLQVQLIEAFGAGVYALTLASHADLWWRRREQRSHAWLALS
                     AFGALLVNLTGAAIRVAPAAYATALLGLNMLGVAVALISLYELAEAIGGKRPGRLARA
                     MEAATLVPILLLPVLPHAVLLAALYLTGVLFLLAAMFRAARHARSGDPESHVIALGLT
                     FLFTTLLYDILSEMQVLVRVEGMPVLGFTVLYLAAARALSLRYEREHRELLELRGKLE
                     TRVQQRTTELEAANQRLEQLSRTDALTGLANRRCFVEAAAAYLRASPARLLMIDIDHF
                     KRINDTHGHETGDAALRAVADALGQGLRSGELLARWGGEEFIALLEDADDASARAERM
                     RAAVAATCVQSGDAALRLSASFGLARVARGGALDAAIARADEALYRAKREGRDRVVTD
                     TAGSDAG"
     gene            complement(5794..>6890)
                     /locus_tag="PK818_07970"
     CDS             complement(5794..>6890)
                     /locus_tag="PK818_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HQY82702.1"
                     /translation="RLVIDIGGGSTECIIGSGMDAIERESLQAGCVASTRRFFPNGKL
                     SRKRWKDALTEVTAEFQQFASAYRALGWDEAIGSSGTNKAIGEICAALKLTKGAVTAE
                     ALPLLRERLLQAERIEDIDLPGLSVDRRPIIAGGLLVLEAVFAALGLQRMAVSKAALR
                     EGVLHDMLGRGGADDPRDVSVAALVQRYGIDAAQARRVEASALHLFDQVARTWALDGD
                     DRRMLARAARLHEIGLAIAHSGYHVHGAYILENSDIAGFSQQEQRFLAALVRTHRRGI
                     PKAAFEMIPDRLLGNARRTAALLRLAVLLHRSHDNERIPDLTLRADAGQLALALDKRW
                     LQARPLLRADLEGEPEDMLGLGIQLRIAAQ"
BASE COUNT          956 a         2409 c         2470 g         1055 t
ORIGIN      
        1 cgatgcagaa gcaccagtac gcgcgctgga accagcaggc gttcgacggc gtgatcggcc
       61 attaccacgt gctgccggag cagaaggtcg ggcagttgtc gcgcggcgaa cgcgccggct
      121 tcaacctcgc gctggcattg gcgcagcgac cggaactgct ggtgctggac gaacccaccc
      181 tgggcctgga cgtggtcgcc aagcgcgcgt tcctggagtc gctgctgtac agcaatgccg
      241 ccgacgactg cacggtgatc tattgctcgc accagatgga ggagatcgag cgcgtcgccg
      301 acaacctgat catcctcgag cgcggccagc tgaagaacat gtcggcgccg gaggacttca
      361 ccgcgcgggt cagccactgg gtggccgacg tgccgttcaa gggccccgaa ctgcacagcg
      421 tgccgggcct gctggaagtg cagcgcctcg acggcctgca ccactacctg gtgctggacc
      481 aggacgccgg cttcgaacga ttcctgcgcg ccagcggcgc gcgcaacgtg caatcgatgc
      541 cggtcagcct ggaccgcgcc gtcaatgcct tccttgccaa gaaccatgct gcgccggctg
      601 cggcctgacg cgcgcgatag aggacaccaa catgatcgat ctcttcaagg gcgagctgct
      661 gcgcttccgc ctgtgggcgc tggtcgccgc cgtcatcaac ctcgtggcgc tgggtttcat
      721 gagtaggctg gtggacctgg cccagcaacc gctggtcgtc taccaggtcg ccagcatggt
      781 ctatgcggtg gcgggggcgc tgctgggcct gttccagatg ggcagctatc gcaagcccaa
      841 ccagtggctc aacctgctgc atcggccgct gcaccggctg cgcatcgccg gcgcgctgac
      901 cgctgccggc gtagtggtgc tgctggtggc ggtggcgctg ccgatcgcgc tggtcgcggt
      961 gtaccaggaa tcgctgacgc cgcgggtggt cgacatgcgc cactggctgc tgccgattgc
     1021 cgcgtggctg atcgcggtgt ccggctacct ggccggtgcc tacgcgatgg tcggcgaccg
     1081 ccgttacgca ttcaccggct tcctgctgcc gaacctgttc atgtattcgc aggcgtccgg
     1141 cgtggccatg ctcggcgtgc acctgttcgc gatcgcattc ctggtcgtgc tgctggcgat
     1201 cgcgttcaag ccggacccgg ccgcaccctc gcgcaacccg ctggcggtgc tcgcggtcgc
     1261 cctgccggtg caggtgggtg cgtatttcct ggcgtggatg ctgggctatg gggtcgaact
     1321 gggctggacc gtcagcggca cccatccgct cggcatgccg gtgccgccga agggcggcta
     1381 catcgaggcc gaccgcgccg aaggcaagga catgctgttg ctcggcatcg atgccagccg
     1441 ctcgccggag gctgcgttgt ggcgcgaaca ggtcgcactc tcggacgtgc atacgcgcta
     1501 tccgctgcgc aacctgcctg tgcgcaacac gctgaccaac cccgcgccga tggaattcga
     1561 cgatggcgag aacagcgtgc gctgggtgtt cagccatgac cgcatgcgct tcgtcggcta
     1621 cggcaccctg gacaagcggg cgcgtggcga actgggcgtg ggtgaaaccc gtgccgcatt
     1681 ccccgcgccg accctgcaat atgccgccgg ctttctgttc aacgccaatg cggcctatca
     1741 atacgatgcc ggccagcagc gcatcttcga acgcatccgc ctgccgcggg gcgaggtgat
     1801 ggccagcccg ccggaaccgg ccggcgacaa cgtgctggtg ctcagcgacc gcgccgccta
     1861 tttctatccc ggccgcgagg cggtcaatgg ccttgatctg ctgcagccgc tgctgcgggt
     1921 gccgatgccg ggccatgtcg gcaacctcag ccgggtggac gcgatcgaac tgctggatgg
     1981 ttacctggtc tcgtttacct acacctgggg cgtgtggagc ggggagctgc agcagccgtt
     2041 ccagcaactg gtgcgggtgg atgacgcggg caaggtgcat gaagtcgcac gccgcgcgat
     2101 cgacgtcgac ctgccgtccg cctacaccaa ccgcaacacc tggctgtcgc cggtgatccg
     2161 cgcgctttgc ctgggcgcgc gcaacctgtt tgccgcgaat gatccgctga aggcgaaacc
     2221 cgagcgcgtg ccgccgtcgg tgctgtggct cgcggccgcg tgttgcctgc tgtcgctgct
     2281 ggcggcgatc tgggtcagcg gccggcaagt gcattcgacg cgcggtcgct gggcctgggt
     2341 ggtgctgtgc ggcgtggtcg gcctgccggc gctggccagc ctgtggctgc tgtatccgcg
     2401 tcgcgagccg ttgccggtcg cctcgccgac gctcgcctga gcacgcacgg acgcgaggat
     2461 cgacgatgaa catttccagg atcatgtgcg gcgcgctggt ggcatgtgcg ctgctgctgt
     2521 cccctcgcag cgtgcccgcg caggtcgctg acggcctgca ggcggcagtc gccgcccagc
     2581 gcgagatgcc gcgcgcgccg cgcctgccgc gggccgcgtt gctcgaaggc cgcactctgg
     2641 tcgcggtgcg gctgtcgccg gatggcgaac acgtcgccta cctgcgcgaa cgcaaggatt
     2701 cgcgcagcct gtggctgttg cccaccaccg gcggcgccgc ccgcacgctg gtcggcagca
     2761 cccaggcgga gcagctgtgg tggtcgcgcg atgggcgttg gctgttcctg cgcgcgccgg
     2821 gcacgctgac catggttggg gtggatgggc gcagcggtgt gcgcgtgccg ctgggcggtt
     2881 ccggacggcg gcaggtggtc aaggtcgatc cctcgcaacc ggcggccgtg ttgctgcgcg
     2941 agcgggtgcg cgcgggcgcc ggcgagcgct ggcggatcgt gcgcttggat gtgcatggca
     3001 agcgcacgtt gctgcgcgag gacccgcgct ggatccacga cctggcgctg gatgcgcgcg
     3061 gccggatcgt cgcattggcg cgcttcgagg gcgaccacga cgcgctgcat cgcgtcggtg
     3121 cggatggccg gttgcgcgag gtgctgcgcc tgcggccgat gcagcgcatc gacctgctgg
     3181 cgacgctggc cgatggcagc ctgctgctgg aaggcaatcc cggcaatggc gccgaaggca
     3241 acttccgccg cgtgctgcgc ctgggcgcgg acgaccggct gcagacgctg caccaggatc
     3301 cgcgcggcga ggccgacctc gacgacgtgg tgctcgatcc gcgcacgcaa ttgccggtgg
     3361 cggcgagtta tcgcagcacg gtcgccgcca cccatggcat cggcagcgcg caggcaacgg
     3421 tggccgacat cgcgcgcagg ttcccgggcc gcgaccttgg cctgcaggtg ggcagcggcc
     3481 ccgccgcgcg ctggctggtc agcgaacgcg cggccacgtt gcgcgatccg cggctgcacc
     3541 tgtacgaccc gcgcaacggc agcctgcggc ggatcctgga cgagccaggc atcggcacga
     3601 agtcaccgcc ggaagccgcg ctggcgcgca agctcgcgat cgcctacacc gcttccgacg
     3661 gcatgcgcgt gcacgggttc gtgctgctgc cgccgggcgt ggacgtcgcg cgcgcgccgc
     3721 tggttacgca ggtgcacggc ggtccgatca accatttccg cgccgggttc gatggcaccg
     3781 cgcagttcct ggccaatcgc ggctacgtgg tgttccagtc gaatttccgc ggctcaaccg
     3841 gccacggacg cgactacgtg tttgccccga agggcgacta cggcaacggc cgcgtgcagc
     3901 aggacatcgt cgaaggcgtg cgctggctgc tggcgcgcgg catcggcgat gcaaagcgcg
     3961 tcggcatcgt cggccattcg ttcggcggct attccacgct gctggggctg acattccagc
     4021 cggacctgtt caaggtcggc gtggccggcg cgccgccggc ggacctgggc tggtcgatgc
     4081 gctggctggt ccgctccggt tcccagggcg acctgccgga ccgttcgctg cggcacaccc
     4141 tgcgcgcgct gtcgctggac agcaccgacc ccgccaccca tgcgcgcctg catgcgcagt
     4201 cgccgcaggc gaatgcggcg aagatgcgcc ggccgctgct gatcatcgcc ggcggcgcgg
     4261 accgcacggt ggcgatccgc gaagtggcga actacagcgc taccctgaag gcattggggc
     4321 gtccgctgac cttgctggtg gagccgaacg gcggccattc gccggtcgat ccggtcccgc
     4381 gcgaggccta cctgttcgcg atggaaacca tgctgcagcg ccatcttggc gggcctgcac
     4441 cggaacaacc ggggcagcgg ttgcgcgcct acctgcgcgc gaacctgcgc ctggccggac
     4501 cggaattcac caccttcgcg gcgccgccca ggtaggcgct ggcacgcctc agccggcgtc
     4561 gctgcccgcg gtgtccgtca ccacgcggtc gcggccttcg cgcttggccc ggtacagcgc
     4621 ctcgtcggcg cgcgcgatcg ccgcatcgag cgcgccgccg cgggcgaccc gggccagccc
     4681 gaacgaggcg ctgagccgca gcgcggcgtc accggactgc acgcaggtcg ccgccaccgc
     4741 cgcgcgcatg cgttcggcgc gcgcgctggc gtcgtcggcg tcctccagca gggcaatgaa
     4801 ctcctcgccg ccccagcgcg ccagcagttc gccgctgcgc agtccctggc ccagcgcatc
     4861 cgcgaccgcg cgcagggcgg cgtcgccggt ttcgtggcca tgggtatcgt tgatgcgctt
     4921 gaaatggtcg atgtcgatca tcagcagccg cgccggagat gcgcgcaggt acgcggcggc
     4981 cgcctccacg aagcagcggc ggttggcgag gccggtcagc gcatccgtgc gcgagagctg
     5041 ctcgaggcgc tggttggcgg cctccagttc ggtggtgcgt tgctgcaccc gggtttccag
     5101 cttgccgcgc agttccagca gttcgcggtg ttcgcgctcg tagcgcaggc tcagcgcgcg
     5161 cgccgcggcc aggtacagca cggtgaagcc gagcaccggc atgccttcga cccgcaccag
     5221 cacctgcatc tcggacagga tgtcgtacag cagggtggtg aacaggaagg tcaggccgag
     5281 cgcgatcacg tgcgactcgg ggtcgccgga acgcgcgtgg cgcgcggcgc ggaacatcgc
     5341 cgccagcagg aacagcacgc cggtcaggta cagcgcggcc agcagcacgg cgtgcggcag
     5401 caccggcagc agcaggatcg ggaccagcgt tgccgcctcc atcgcccgcg ccagccggcc
     5461 gggacgcttg ccgccgatcg cctcggccag ttcgtacagg ctgatcaggg cgaccgccac
     5521 ccccagcatg ttcaagccca gcaacgcagt ggcgtaggcc gccggcgcca cccggatcgc
     5581 cgcgccggtc aggttcacca gcagtgcgcc gaacgcggaa agtgccagcc atgcgtgcga
     5641 gcgctgctcg cgccggcgcc accacaggtc ggcatgcgag gccagggtca gcgcatacac
     5701 gccagccccg aaggcttcga tcaactggac ctgcagcggg acggccattg ccgcaggata
     5761 ccgccgccag cgcgcggtgg ccgggccggg gcgctactgc gccgcgatcc gcagctggat
     5821 gccgagcccc agcatgtcct cgggttcgcc ctcgaggtcc gcgcgcagca gcgggcgcgc
     5881 ctgcagccag cgcttgtcga gggccagcgc aagctgcccg gcatccgcgc gcagggtgag
     5941 gtcggggatc cgctcgttgt cgtgcgaacg gtgcagcagc accgccagcc gcagcagcgc
     6001 ggcagtgcgg cgcgcgttgc ccagcaggcg gtcggggatc atctcgaacg cggccttcgg
     6061 gatcccgcgg cgatgggtgc gcaccagcgc ggcgaggaag cgctgctcct gctgcgagaa
     6121 gccggcgatg tccgagttct cgaggatgta ggcgccgtgc acgtggtagc cgctgtgggc
     6181 gatcgccagc ccgatctcgt gcaggcgcgc cgcgcgcgcc agcatgcggc ggtcgtcgcc
     6241 atccagggcc caggttcgcg cgacctggtc gaacaggtgc agggcgctgg cctcgacccg
     6301 cctggcctgg gcggcgtcga tcccgtaacg ctgcaccagc gccgccaccg acacgtcgcg
     6361 cgggtcgtcg gcgccgccgc ggccaagcat gtcgtgcagc acgccttcgc gcagcgcggc
     6421 cttgctcacc gccatccgct gcaggccgag cgcggcgaat accgcctcca gcaccagcag
     6481 gccgccggcg atgatcgggc gccggtccac ggacaggccg ggcaggtcga tgtcctcgat
     6541 gcgttcggcc tgcagcaggc gctcgcgcag cagcggcagc gcctccgcgg tcaccgcgcc
     6601 cttggtcagc ttgagcgcgg cgcagatctc gccgatcgcc ttgttggtgc cggacgagcc
     6661 gatcgcctcg tcccagccga gcgcgcggta ggccgacgcg aactgctgga actcggcggt
     6721 gacttcggtc agcgcgtcct tccagcgctt gcgcgacagc ttgccgttgg ggaagaagcg
     6781 gcgggtgctg gcgacgcagc cggcctgcag gctctcgcgc tcgatcgcat ccatcccgct
     6841 gccgatgatg cattcggtgg aaccgccgcc gatgtcgatc accagccgca
//
LOCUS       DAORUK010000113         6780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_12955_length_6780_cov_19.025725, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000113 DAORUK010000000
VERSION     DAORUK010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12955_length_6780_cov_19.025725"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..2931
                     /gene="hrpB"
                     /locus_tag="PK818_07975"
     CDS             295..2931
                     /gene="hrpB"
                     /locus_tag="PK818_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011050223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HQY82703.1"
                     /translation="MHFPINDLQPEIRASLQDHTRLVLEAPPGAGKTTQVPLALLDAP
                     WLAGRKIVMLEPRRVAARAAAGFMAKQLGEAVGETVGYRIRFENKVSTNTRIEVVTEG
                     ILTRMLQDDPLLEGVGAILFDEFHERHLAGDLGLALALDVQAGLREDLRIVAMSATLD
                     GERLAGFLDAPRLSSAGRSFPVAIAHYPARRDEALEHQARRAIEHALSTHPGDVLVFL
                     PGQREIAKVAALLANTHAEVQPMHAAGAARPDAGELLFPQTGLPAPIRARAIHGALEA
                     TPRRPATPPDGFEVLPLHGELPVEQQARVLQPAPDGRRRVVLATNVAESSVTLPGVRV
                     VIDSGLAREPRFDPNSGFSRLDSVAISQASADQRAGRAGRVAEGFAYRLWPASQRLEP
                     QRKPEIAQVELAALALELAAWGSAALRFVDPPPPGALAAGRDLLQRLDALDAGKLTAR
                     GRRMLALGTHPRLAALLLASSDPARIALACDIAALVEARDPLRSRSDALAERWQALAA
                     FRNGRVPADANRSALAAIDAAAKQWRRRLRCDATPPASAPAHAIGDLLAHAFPDRIAR
                     QHPQDPRRYQLANGRMARLFDDSALYGEPWLAISELRFEAKDALLLRAAPVDEVRLRA
                     EFATRFSDADEVRWDAEKRALRSERIERFDGIVLAAKSAGRVDPALAARALTDAVREL
                     GLSVLPWSEALSQWRIRVQCLRAWMPELGLPDLSDAALLASLERWLQPAFAGKTRLDA
                     LDGGELAEALKSGIDWRLRQRIDPLAPTRIVVPSGLERGIDYRVDDHGEPAPPVLAVK
                     LQELFGLAQTPRIADGRVPLLLHLLSPGGKPLQVTADLHNFWASTYAEVKKEMKGRYP
                     RHPWPDDPWNAVATHRAKPRGT"
     gene            3032..3382
                     /locus_tag="PK818_07980"
     CDS             3032..3382
                     /locus_tag="PK818_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82704.1"
                     /translation="MDKLDKAQDRALELLGEIGDGLRKAVPGKAIQWVETGAAIGVVK
                     TGARVATKFVRRNPILATAAIAGAGLLWYAARRRARQAEAGPIEGSATRVEATAGSGA
                     PRAPRKRRTETDAG"
     gene            complement(3404..4528)
                     /locus_tag="PK818_07985"
     CDS             complement(3404..4528)
                     /locus_tag="PK818_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HQY82705.1"
                     /translation="MPHTVIRGPTTGGTILVVDDQAANVRVVGALLGRNGYEVMTASS
                     GPEALAAARTRAPDLILLDMVMPGMDGFDLLAALRSEPALRAVPAVFLTAAQDRDLLL
                     RAFEAGAVDYVTKPFMPGELLVRVGTHIGLKQARDRLERVARERQELVNLVAHDLKNP
                     LTCVLFASQMLRQPDCLPERVPRYLQIIEDSAQDAVGYIRKYLEGQARALDSAQARAA
                     TAATACLQETVDWLARRYDLQLEGCGMHLALRVPERSGRVAIEPMVLRQVAGNLVSNA
                     MKYAQSGGELELVVRASAPGFWQLLARDRGPGIPAEKQRQLFKPFQRLGAQDPSDSLS
                     SGLGLSLARQIIAEAGGQLWYEDREGGGACFVIELPQAAG"
     gene            complement(4674..>5201)
                     /locus_tag="PK818_07990"
     CDS             complement(4674..>5201)
                     /locus_tag="PK818_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQY82706.1"
                     /translation="FNKPFGVLCQFTDRSTPARPTLAGFGLPAGVYPAGRLDFDSEGL
                     LLLTDDGRLAHRLTDPRHKQPKTYWVQVEGDPGTAQLEALRTGVVLNDGPTLPARVRR
                     VDAPPLWPRDPPVRVRKTVPDAWLEITLTEGRNRQVRRMTAAVGLPTLRLVRAAIGGH
                     GLDTLAPGGWRQVQA"
     assembly_gap    5202..5301
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5327..6154)
                     /locus_tag="PK818_07995"
     CDS             complement(5327..6154)
                     /locus_tag="PK818_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQY82707.1"
                     /translation="MIRPLALACCLALLAGCAGTPTAATPAATVAATSFDGVLAGSWR
                     DPANVARDRYRHPAETLAFFGLAPTHTVIEITPGGGWYSELLAPYVASGGRYIGAMPA
                     AAPDSGGGKRNAALRQKLAGNPAYGAARLVEFDPKAPVFGPAASADVVLTFRNLHNWL
                     AAGTTEYHLRAAYDALKPGGVLGVVDHRARPGTDIETSKKSGYVSEQLVIDLAGEVGF
                     ALEARSEVNANPKDDTDHPNGVWTLPPVNRHDAADDATYKAIGESDRMTLRFRKPAR"
     gene            complement(6202..>6780)
                     /locus_tag="PK818_08000"
     CDS             complement(6202..>6780)
                     /locus_tag="PK818_08000"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HQY82708.1"
                     /translation="TDLFPIMELGTSAKMLSIVPLMNGGGLFETGAGGSAPKHVQQFT
                     EEDYLRWDSLGEFLALAASLEHLANTTGNARAQVLADALDAANAEFLDHDRSPGRKLG
                     TIDNRGSHFYIALYWAQALAGQAADPELAALFAPLAKVLAESEEKIVAELVAVQGKPV
                     DIGGYYQPDPSRLSAAMRPSPTFNAALAMLAG"
BASE COUNT          920 a         2365 c         2443 g          952 t
ORIGIN      
        1 tcgcggtcgt ggtcgaagct gtcgtgggtg aggttggcgc cgatgtcgat gagatccatg
       61 tgcgcagttt agcgaggcac ggatcgaccc tcggcaggca catcgtgcga cggaatctcg
      121 acgggacggg cgcggcgcgg ggtgtcgcgt caagccggcc atggatggcc ggcgcgcgag
      181 tcagggcagg acgccctgac cgagcggaga gcgatacccc gcgccgcgcc cggtccgtcc
      241 ggcgttgatc gccatgtgtc gggtcggact gcgcggccct cctacaatgc gcacatgcat
      301 ttccccatca acgacctgca gccggagatc cgcgcctcgt tgcaggacca cacgcggctg
      361 gtgctggaag cgccgcccgg cgccggcaag accacccagg tgccgctggc gctgctggac
      421 gcaccctggc ttgcgggcag gaagatcgtg atgctggagc cgcgccgggt cgccgcgcgt
      481 gcggccgccg gcttcatggc caagcagttg ggcgaagcgg tgggcgagac cgtcggctac
      541 cgcatccggt tcgagaacaa ggtgtcgacg aacacccgca tcgaggtcgt caccgaaggc
      601 atcctcaccc gcatgctgca ggacgacccg ctgctggaag gcgtcggcgc gatcttgttc
      661 gacgaattcc acgaacgcca cctggccggc gacctgggcc tggcgctggc gctggacgtg
      721 caggcaggac tgcgcgagga cctgcgcatc gtggcgatgt cggccacgct ggacggcgag
      781 cggctggcgg gcttcctcga cgcgccgcgc ctttcgagtg cgggccgcag ctttccggtc
      841 gcgatcgcgc actacccggc gcgccgcgac gaggcgctgg aacaccaggc aaggcgcgcc
      901 atcgaacacg cgctgtccac gcatcccggc gacgtgctgg tgttcctgcc ggggcagcgc
      961 gagattgcga aggtggcggc gctgcttgcg aatacgcatg cggaagtgca gccgatgcac
     1021 gctgcgggag cggcgcggcc ggacgccggg gaattgctct tccctcaaac ggggcttcct
     1081 gccccgattc gggcgcgcgc catccatggc gcgcttgaag caacaccccg gcgcccggcc
     1141 acgccgcccg acggattcga agtcctcccc ctgcacggcg agctccccgt cgaacagcaa
     1201 gcgcgcgtgc tgcaacccgc gcccgacggc cggcggcggg tggtgctggc caccaacgtg
     1261 gccgagtcct ccgtcaccct gcccggcgtg cgcgtggtga tcgacagcgg gctggcgcgc
     1321 gagccgcgct tcgatcccaa cagcggcttc tcgcggctgg acagcgtggc gatttcgcag
     1381 gccagcgccg accagcgcgc cggccgcgcc gggcgcgtgg cggagggttt cgcgtaccgg
     1441 ctgtggccgg catcgcagcg gctggagccg cagcgcaagc ccgagatcgc gcaggtggaa
     1501 ctggccgcgc tggcgctgga gctggcggcc tggggcagcg ctgcgctgcg cttcgtcgat
     1561 ccaccaccgc ccggtgcgct ggcggccggc agggatctgc tgcagcggct ggatgcgctg
     1621 gatgccggca agctcaccgc gcgcggccgg cgcatgctgg cgctgggcac gcacccgcgg
     1681 ctggcggcgc tgctgctggc ctccagcgac ccggcgcgga ttgcgctggc ctgcgacatc
     1741 gccgctctgg tggaggcgcg cgatccgctg cgcagccgct cggatgcgct ggccgaacgc
     1801 tggcaggcat tggcggcatt ccgcaacggg cgcgtgccgg ccgatgccaa ccgttccgcg
     1861 ttggccgcga tcgatgccgc agcgaaacag tggcggcgcc ggctgcgttg cgacgccacg
     1921 ccgcctgcgt ctgcacccgc gcatgcgatc ggcgacctgc tggcgcacgc gttccccgac
     1981 cgcatcgccc gccagcatcc gcaggacccg cgtcgctacc agctggccaa cgggcggatg
     2041 gcgcggctgt tcgacgattc cgccttgtac ggcgaaccgt ggctggcgat cagcgagttg
     2101 cgtttcgagg ccaaggacgc gctgctgctg cgcgccgcgc cggtggacga ggtgcggctg
     2161 cgggccgagt tcgcgacccg tttttccgat gccgacgagg tgcgctggga tgccgaaaag
     2221 cgggcgctgc gcagcgaacg catcgagcgt ttcgacggca tcgtgctggc ggcgaaatcg
     2281 gcggggcggg tcgatcccgc gctggcggcg cgggcgctga ccgacgcggt gcgcgaactc
     2341 ggcctgtcgg tgttgccgtg gagcgaagcg ctttcgcagt ggcgcatccg cgtgcagtgc
     2401 ctgcgcgcgt ggatgccgga gctgggcctg ccggatcttt ccgatgccgc gctgctggcg
     2461 tcgctggagc gctggctgca gcctgcgttc gccggcaaga cccggctgga tgcgctggat
     2521 ggcggggagc tggccgaagc actgaagtcc ggcatcgact ggcgcctgcg ccagcgcatc
     2581 gacccgctgg cgccgacccg catcgtggtg ccgtccgggc tcgagcgcgg catcgactac
     2641 cgagtggacg accacggcga gccggcgccg ccggtgctgg cggtgaagct gcaggaactg
     2701 ttcgggctgg cgcagacccc gcgcatcgcc gacggccggg tgccgctgct gctgcacctg
     2761 ctctcgcccg gcggcaagcc gctgcaggtg accgccgacc tgcacaactt ctgggccagc
     2821 acttatgccg aggtgaagaa ggagatgaag ggccgctacc cgcgccatcc gtggccggac
     2881 gacccgtgga atgcggtggc gacccaccgg gcgaaaccgc ggggaacctg acccctgccg
     2941 gccgctgaac gccgggggca gcgctcacgc ctcatgaacg cgcggatacc gagactgccg
     3001 tcaaactccg ggattcattc gaggatcgac catggacaag ctggacaagg cacaggaccg
     3061 cgcgctggaa ctgctcggcg agatcggcga cggcctccgc aaggcggtac cgggcaaggc
     3121 gatccagtgg gtcgagaccg gcgccgcgat cggcgtggtg aagaccggtg cgcgggtcgc
     3181 gacgaaattc gtgcgccgca acccgatcct ggccacggcc gcgatcgccg gtgccggcct
     3241 gctctggtac gccgcgcgcc gacgtgcccg ccaggccgaa gccggaccga tcgagggcag
     3301 tgcgacccgg gtcgaggcca ccgccggcag cggcgcaccc cgcgccccgc gcaagcgccg
     3361 caccgaaacc gacgccggct gaccgcgccg cccgctgccg gcgtcatccg gcggcttgcg
     3421 gcagctcgat cacgaagcag gcgccgccgc cttcgcggtc ctcgtaccac agctgtccgc
     3481 cggcttcggc gatgatctgc cgcgccagcg acaggccaag accgctggag aggctgtcgg
     3541 aggggtcttg cgcgcccagc cgctggaacg gcttgaacag ttgccgctgc ttctcggcgg
     3601 gaatcccggg gccacgatcg cgcgccagca actgccagaa gcccggtgcc gaggcgcgca
     3661 ccaccagttc cagttcgccg ccggactgcg cgtacttcat cgcgttgctg accaggttcc
     3721 cggccacctg gcgcagcacc atcggctcga tcgcaacccg tccgctgcgt tcggggacac
     3781 gcagcgccag gtgcatgccg cagccctcca gttgcaggtc gtaacgccgc gccagccagt
     3841 cgacggtctc ctgcaggcag gcggttgccg ccgtcgctgc gcgtgcctgc gccgaatcca
     3901 gtgcgcgggc ctggccttcc aggtacttgc ggatgtagcc caccgcgtcc tgcgcactgt
     3961 cctcgatgat ctgcaggtag cgcggcaccc gctccggcag gcagtccggc tggcgcagca
     4021 tctggctggc gaacagcacg caggtcagcg ggttcttgag gtcgtgggcc accaggttga
     4081 ccagctcctg gcgttcgcgc gccacccgtt ccaggcggtc gcgggcctgc ttcaggccga
     4141 tgtgggtgcc cacccgcacc agcagttccc cgggcatgaa cggcttggtg acgtagtcca
     4201 ccgcaccggc ctcgaacgcc cgcagcagca ggtcgcggtc ctgcgcggcg gtcaggaaca
     4261 ccgccggcac cgcgcgcagc gccggctccg accgcagcgc cgccagcagg tcgaacccgt
     4321 ccatccccgg catcaccatg tcgagcagga tcaggtccgg cgcccgcgtg cgcgccgcgg
     4381 ccagcgcttc cggtccgctg ctggcggtca tcacctcgta gccgttgcgg ccaagcaggg
     4441 ccccgaccac ccgcacgttc gcggcctggt cgtcgaccac caggatggtg ccgccggtcg
     4501 tggggccacg gatcaccgtg tgtggcatgg agggcgtgtc cggacgccgg cgcatggaaa
     4561 gggcatccag tgtcggcgga tgacgtgccg ccgacaaccg gcattcgccg caagcccggg
     4621 tcgggacctg cctgcccacc gggacgatcc acccgcaggc ctgggacccg gcttcaggcc
     4681 tggacctgcc gccatccgcc gggggccagt gtgtccaggc catggccgcc gatcgccgca
     4741 cgcaccagcc gcagggtggg caggccgacc gcagcggtca tccgtcgcac ctggcggttg
     4801 cggccctcgg tcagggtgat ctccagccag gcatccggca cggtcttgcg gacccgcacc
     4861 ggcgggtcgc gcggccacag cggcggggcg tcgacgcggc gcacgcgtgc cggcagggtg
     4921 gggccatcgt tcagcaccac cccggtgcgc agtgcctcga gttgcgccgt cccggggtcg
     4981 ccttcgacct gcacccagta ggtcttcggc tgcttgtgcc gcggatcggt caggcggtgc
     5041 gccaggcggc cgtcatcagt gagcagcagc agcccctcgg aatcgaaatc caggcgtccg
     5101 gccggataca cccccgccgg caggccgaag ccggcaaggg tggggcgcgc cggggtgctg
     5161 cggtcggtga actggcacag caccccgaac ggcttgttga annnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn ngccggcagc ggcgtccgcg ggtcttctag cgggcgggct
     5341 tgcggaagcg cagggtcatg cggtcgctct cgccgatcgc cttgtacgtg gcatcgtcgg
     5401 cggcgtcgtg gcggttcacc ggcggcagcg tccacacccc gttcggatgg tcggtgtcgt
     5461 ccttcggatt ggcgttgact tcgctgcgcg cctccagcgc gaagccgact tcgccggcca
     5521 ggtcgatcac cagctgttcg ctgacgtagc cgctcttctt cgacgtctcg atgtcggtcc
     5581 cgggcctggc gcgatggtcg accacgccca gcacgccgcc gggcttgagc gcgtcatagg
     5641 cggcgcgcag gtggtattcg gtggtgccgg cggccagcca gttgtgcagg ttgcggaagg
     5701 tcagcaccac gtcggcgctg gcggccgggc cgaacaccgg ggccttgggg tcgaactcga
     5761 ccagccgcgc cgcgccgtag gccgggttgc ccgccagctt ctggcgcagg gcggcattgc
     5821 gcttgccgcc gcccgagtcc ggcgcggccg cgggcatggc gccgatgtac cggccgccgc
     5881 tcgccacgta cggcgccagc agttcgctgt accagccacc gcccggggtg atctcgatga
     5941 ccgtatgggt gggcgccagt ccgaagaacg ccagggtctc ggccggatgg cgatagcggt
     6001 cgcgggcgac gttggcaggg tcgcgccagc tgccggcgag gacgccatcg aacgacgtgg
     6061 ccgcgacggt cgctgccggg gtggcggcgg tgggcgtacc ggcgcagccg gccagcaggg
     6121 cgaggcagca ggcgagggcg aggggacgga tcatgcgggc gctccgggga aggacgggcg
     6181 cccagcctac cgatgccggg gtcagccggc cagcattgcc agtgccgcgt tgaaggtcgg
     6241 gcttggccgc atcgcggcgg acagccgcga cgggtccggc tggtagtagc cgccgatgtc
     6301 caccggcttg ccctgcaccg ccaccagttc cgcgacgatc ttctcctcgc tttccgccag
     6361 caccttcgcc agcggcgcga acagcgcggc cagttcggga tccgccgcct gcccggccag
     6421 cgcctgcgcc cagtacagcg cgatgtagaa atggctgccg cggttgtcga tggtgccgag
     6481 cttgcggccc ggcgagcggt cgtggtcgag gaactccgcg ttggccgcgt ccagcgcgtc
     6541 cgccagcacc tgcgcgcggg cgttgccggt ggtgttggca aggtgctcca gcgaggccgc
     6601 cagcgccagg aattcgccca gcgaatccca gcgcaggtag tcctcctcgg tgaactgctg
     6661 gacgtgcttg ggcgcgctgc cgccggcgcc ggtctcgaac aggccaccgc cgttcatcag
     6721 cggcacgatc gacagcatct tcgcgctggt acccagctcc atgatcggga acaggtcggt
//
LOCUS       DAORUK010000114         6635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_13384_length_6635_cov_21.168541, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000114 DAORUK010000000
VERSION     DAORUK010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13384_length_6635_cov_21.168541"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /gene="fghA"
                     /locus_tag="PK818_08005"
     CDS             complement(<1..560)
                     /gene="fghA"
                     /locus_tag="PK818_08005"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005413342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HQY82709.1"
                     /translation="MQRIEHRACFGGWQDVYEHASATLGCTMRFAIYLPPQAADGPVP
                     VLYWLSGLTCTEQNFITKAGAQRYAAEHGIAIVCPDTSPRGDDVVDAAGYDLGMGAGF
                     YVNATQAPWSVHYRMYDYVVEELPALVAANFPVTEARAISGHSMGGHGALVIALRNPG
                     RYRSVSAFAPIVAPSQVPWGQKAFAA"
     gene            complement(560..1669)
                     /locus_tag="PK818_08010"
     CDS             complement(560..1669)
                     /locus_tag="PK818_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HQY82710.1"
                     /translation="MKSRAAVAFAAGQPLQIVEIDVAPPKAGEVLVKITHTGVCHTDA
                     FTLSGDDPEGLFPCVLGHEGAGIVVEVGEGVTSVAPGDHVIPLYTAECGECLFCKSGK
                     TNLCTSVRATQGKGVMPDGTTRFSYDGQPLYHYMGCSTFSEYTVVAEVSLARINPEAN
                     HEQVCLLGCGVTTGLGAVQNTAKVQEGDSVAVFGLGGIGLAVIQGAQRAKAGRIIAVD
                     TNPSKFGMARDMGATDCVNPKDFDKPIQQVIVEMTGWGVDHSFECIGNVNVMRAALEC
                     AHRGWGQSVIIGVAGAGQEISTRPFQLVTGRKWLGTAFGGVKGRSQLPGMVEEAMAGE
                     IRLAPFVTHTMGLDEINIAFDLMHEGKSIRSVVHF"
     gene            complement(1749..4463)
                     /gene="ppc"
                     /locus_tag="PK818_08015"
     CDS             complement(1749..4463)
                     /gene="ppc"
                     /locus_tag="PK818_08015"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008207891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HQY82711.1"
                     /translation="MTPSASPLREKLEFTGTDAPLRDDVRRLGAMVGEMLAEQVSEQF
                     LAQVEQLRRIAIARREQGEPVDTLAARLAGVPLADAEALVRAFAAYFGATNLAERVHR
                     IRRRRDYQRQGGAPQPGGLHAVLHELRVAGVRLAELQALLARMHVEPVFTAHPTEAIR
                     RALLLKERTVVERLVADIDRTRTPPERGADDARIRQALTTAWQTTEAPPHKPSVADEV
                     EHIGFYLSSVLYRVLPVFYESFADAVEAVYGERIALPPLLRFGSWVGGDMDGNPNVGA
                     ASVGDALAAQRALALSCYQADLRALGDSLTQSRGRIGFDAEIETRAAAHRALFAEGAV
                     RSRPRLADMPYRQLLWAMRARLHATAGDSPGGYPDAAAFIDDLELIDASLARHQGDHA
                     GRFALQRVLWRAKTFGLHMAALDLRQDSAAHDEALAALEGDIDWAGLPLAARIERLHA
                     VIGGAPVVAPAAPAAQATLDVFRSVRALRQSLGEHAFGPYIISMSRTAADALAVLALA
                     RVAGCTGGDAVPLDVAPLFETVDDLDAAPAVMRALFADPAYRAHLAARGDRQTVMLGY
                     SDSAKDGGLLASRWALQRAQVELLELARDAGVAIVFFHGRGGSVSRGGGKTERAIIAA
                     PRGSVDGRMRVTEQGEVIHRKYGIRALALRNLEQMTGAVLRASLRPRPPEPREAAWRT
                     IAAELATVSRTHYRALVHAHPDFPAYFRAATPIDVIERLHIGSRPSRRRDGGIANLRA
                     IPWVFAWSQNRSGLTGWHGLGTALRHGIDVHGLDAMADMARDWPFFAALVDDVEMLMA
                     KSDLAIFECYSRLAGTQHEAFFPAIAAEFERTREAILAIKDRDELLADDYRLRLSIRL
                     RNPYVDPISLLQVDLLQRWRAGDRADEDLLRALVATVNGISAGIQNTG"
     gene            complement(4556..5920)
                     /locus_tag="PK818_08020"
     CDS             complement(4556..5920)
                     /locus_tag="PK818_08020"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HQY82712.1"
                     /translation="MSGPSSANDTPITDHRQLVDVLASGEKPREAWRIGTEHEKFGFR
                     LDDLRPPTFDGERGIEALLKGLARFGWTPFEEHGHVIALTKGLASVTLEPAGQLELSG
                     APVETLHDTCREVGSHLREVREVADELGLGFLGMGFQPKWRRDEMPWMPKGRYRIMRE
                     YMPKVGTLGLDMMTRTCTVQVNLDYASEADMVKKFRVSLALQPVATALFADSPFTDGK
                     PNGYLSYRSHIWTDTDRDRTGMLDFVFEDGFGYERYVDYLLDVPMYFSYRDGEYIDAS
                     GKSFRTFLRGELDVLPGALPTIRDWNDHMTTAFPEVRLKKFLEMRGADGGPWNRLCAL
                     PAFWVGLLYDDAALDAAWDLVRDFSMAERNALRDDVPRHALKTPFRGGTLRDIAIEAL
                     KIADAGLRRRARADSCGVDESGFIAPLLEIAEAGETPAERKLALFHGAWNGSVDPVFR
                     EFAY"
     gene            6088..>6635
                     /locus_tag="PK818_08025"
     CDS             6088..>6635
                     /locus_tag="PK818_08025"
                     /inference="COORDINATES: protein motif:HMM:NF020653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrinase N-terminal
                     domain-containing protein"
                     /protein_id="HQY82713.1"
                     /translation="MRLLLACCLWLITFAVPAATIERVEPGNWWVGMQHNRVELLVHG
                     NGIGTATPRLVHAGVSIAGVHRTDNPNYLFVTVQLAGDAAPGVFDIEFVEAGKVVATH
                     PWRLDAREPGSAQRRGFDARDAIYLVTPDRFANGDPSNDSVAGMAEAADRADPNGRHG
                     GDIAGIRKHLDYIAGMGFTQLWP"
BASE COUNT          975 a         2378 c         2280 g         1002 t
ORIGIN      
        1 taggcggcga acgccttctg gccccatggc acctgcgacg gcgccacgat cggcgcgaac
       61 gcggacacac tgcggtagcg cccggggttg cgcagggcga tcaccagcgc gccgtggccg
      121 cccatcgaat gcccgctgat cgcgcgggct tccgtcaccg ggaaattcgc cgcgaccagc
      181 gccggcagtt cttcgaccac gtagtcgtac atccggtaat gcacggacca cggcgcctgc
      241 gtcgcgttga cgtagaagcc cgcacccatg ccgaggtcgt agcccgccgc atcgaccaca
      301 tcgtcgccgc gcgggctggt atccgggcag acgatggcga tgccgtgctc cgccgcgtag
      361 cgctgcgcgc cggccttggt gatgaagttc tgttcggtgc aggtcagccc cgacagccag
      421 tacagcaccg gcaccgggcc atccgcggcc tgcggcggca ggtagatcgc gaagcgcatc
      481 gtgcagccga gggtggccga cgcatgctcg taaacgtcct gccagccgcc gaagcaggcg
      541 cgatgttcga tgcgttgcat cagaagtgca cgaccgagcg gatcgacttg ccttcgtgca
      601 tcaggtcgaa ggcgatgttg atctcgtcca ggcccatcgt gtgggtgacg aacggcgcca
      661 gccggatctc gccggccatc gcctcttcca ccatcccggg caattgcgaa cgccccttca
      721 ccccgccgaa cgcggtgccc agccacttgc gcccggtgac gagctggaac ggccgcgtgc
      781 tgatctcctg cccggcaccc gccacgccga tgatcacgct ctggccccag ccacggtgcg
      841 cgcactccag cgcggcgcgc atcacgttga cgttgccgat gcactcgaag ctgtggtcca
      901 cgccccagcc ggtcatctcc acgatcacct gctggatcgg cttgtcgaag tccttcgggt
      961 tcacgcagtc ggtggcaccc atgtcgcgcg ccatgccgaa cttcgaggga ttggtgtcga
     1021 cggcgatgat ccgccccgcc ttcgcccgct gcgcgccctg gatcaccgca aggccgatgc
     1081 cgccgaggcc gaacaccgcg accgaatcgc cttcctgcac cttggcggtg ttctgcaccg
     1141 cgcccaggcc ggtggtgaca ccgcagccga gcaggcagac ctgctcgtgg ttggcttcgg
     1201 ggttgatcct ggccagcgag acttcggcca ccacggtgta ttcgctgaag gtgctgcagc
     1261 ccatgtagtg gtacagcggc tggccgtcgt agctgaagcg ggtggtgccg tccggcatca
     1321 cgcccttgcc ctgggtcgcg cgcaccgagg tgcacaggtt ggtcttgccg ctcttgcaga
     1381 acaggcactc gccgcattcg gcggtgtaca gcgggatcac gtggtcgccg ggcgcgacgc
     1441 tggtgacgcc ctcgccgact tcgaccacga tgcccgcgcc ctcgtggccc agcacgcagg
     1501 ggaacaggcc ctccggatcg tcgccggaca gggtgaacgc gtcggtgtgg cagacgccgg
     1561 tgtgggtgat cttcaccagc acttcgccgg ctttcggcgg ggcgacgtcg atctcgacga
     1621 tctgcagcgg ctggccagcg gcgaaggcga cggcggcgcg tgatttcatt ggcatgttcc
     1681 tcgatggcat cttcgcaatt gtagagcggg gcttgcccca tgctgccgcg acgcccgcgc
     1741 acccggcctc acccggtgtt ctggatcccc gcggaaatgc cgttgacggt ggccaccagc
     1801 gcgcgcagca ggtcctcgtc cgcgcgatcg ccggcgcgcc agcgctgcag caggtcgacc
     1861 tgcagcaggc tgatcggatc gacgtaggga ttgcgcaggc ggatcgacag ccgcagccgg
     1921 tagtcgtcgg ccagcagttc gtcgcggtcc ttgatcgcca ggatcgcctc gcgggtgcgc
     1981 tcgaattcgg cggcgatggc ggggaagaat gcctcgtgct gcgtaccggc cagccgcgaa
     2041 tagcattcga agatggcgag gtccgacttc gccatcagca tctcgacgtc gtcgaccagc
     2101 gccgcgaaga acggccagtc gcgggccatg tccgccatcg catcgaggcc atgcacgtcg
     2161 atgccgtggc gcagcgcggt gccgaggcca tgccagccgg tcaggccgga gcggttctgc
     2221 gaccacgcga acacccacgg gatcgcgcgc aggttggcga tcccgccatc gcggcggcgc
     2281 gagggccgcg aaccgatgtg caggcgctcg atcacgtcga tcggggtggc cgcgcggaag
     2341 tacgcgggga aatccgggtg cgcgtgcacc agcgcgcggt agtgcgtgcg cgagaccgtg
     2401 gcgagttcgg cggcgatcgt acgccaagcc gcctcgcgcg gttccggcgg gcgcgggcgc
     2461 aggctggcgc gcagcaccgc gccggtcatc tgctcgaggt tgcgcaaggc cagcgcgcgg
     2521 atgccgtact tgcgatggat cacctcgccc tgctcggtga cccgcatccg cccgtccacc
     2581 gaaccgcgcg gcgcggcgat gatcgcgcgt tcggtcttgc cgccaccacg gctgaccgaa
     2641 ccgccacggc catggaagaa cacgatcgcc accccggcat cgcgggccag ctccagcagt
     2701 tccacctgcg cgcgctgcaa ggcccagcgc gaggccagca agccgccgtc cttggcgctg
     2761 tcgctgtagc ccagcatcac cgtctggcgg tcgccgcgcg ccgccaggtg ggcgcggtac
     2821 gccgggtccg cgaacagcgc gcgcatcacc gccggcgcgg catcgaggtc atccaccgtt
     2881 tcgaacagcg gcgccacgtc cagcggcacc gcatcgcccc cggtgcagcc ggcgacccgc
     2941 gccagcgcca gcaccgccag cgcatcggcg gcggtccggc tcatgctgat gatgtacgga
     3001 ccgaacgcat gctcgccgag cgactggcgc agcgcgcgca cgctgcggaa cacgtcgagt
     3061 gttgcctgcg cggctggcgc cgcgggcgcc accaccggcg cgccgccgat caccgcgtgc
     3121 aggcgctcga tgcgcgccgc cagcggcagg cccgcccagt cgatgtcgcc ttccagagcc
     3181 gccagcgctt cgtcgtgcgc cgccgaatcc tggcgcaggt cgagcgcggc catgtgcagg
     3241 ccgaaggtct tcgcgcgcca cagcacgcgc tgcagggcga agcggccggc gtggtcgccc
     3301 tggtggcggg ccaggctggc atcgatcagc tcgaggtcgt cgatgaacgc ggccgcatcc
     3361 ggatagccgc ccggactgtc gccggcggtc gcgtgcaggc gcgcgcgcat cgcccacagc
     3421 agttgccggt atggcatgtc ggccagccgc gggcgcgaac gcaccgcgcc ctcggcgaac
     3481 agcgcgcgat gcgccgcggc gcgggtttcg atttccgcat cgaagccgat ccggccgcgg
     3541 ctctgggtca ggctgtcgcc gagcgcgcgc aggtcggcct ggtagcagga cagcgccagc
     3601 gcgcgttgcg ccgccagcgc gtcgcccacg ctggcggcac ccacgttcgg attgccgtcc
     3661 atgtcgccgc cgacccagct gccgaagcgc agcagcggcg gcaacgcgat ccgctcgccg
     3721 tagaccgcct ccaccgcatc ggcgaacgac tcgtagaaca ccggcagcac gcggtacagc
     3781 acgctcgaca ggtagaagcc gatgtgctcc acctcgtcgg ccacgctggg cttgtgcggc
     3841 ggcgcctcgg tggtctgcca ggcggtggtc agcgcctggc ggatgcgggc gtcgtcggcg
     3901 ccgcgctcgg gcggggtgcg ggtgcggtcg atgtcggcga ccaggcgctc gaccaccgtg
     3961 cgttccttca gcagcaacgc gcgccggatc gcctcggtcg ggtgcgcggt gaacaccggc
     4021 tcgacgtgca tccgcgccag cagcgcctgc agttcggcca ggcgcacgcc ggcaacgcgc
     4081 agttcgtgca ggaccgcgtg caggccgccg ggttgcggcg cgccgccctg ccgctggtag
     4141 tcgcggcggc ggcggatgcg gtgcacccgc tcggccaggt tggtggcccc gaaataggcg
     4201 gcgaacgcgc gcaccagcgc ctccgcgtcc gccagcggca cccccgccag ccgcgccgcc
     4261 agcgtatcca ccggctcgcc ctgctcgcgc cgcgcgatcg cgatccggcg cagctgctcg
     4321 acctgcgcca ggaactgctc ggacacctgt tcggccagca tctcgccgac catcgcgccc
     4381 aaccggcgca cgtcgtcgcg cagcggggca tcggtgccgg tgaattcgag tttttcgcgt
     4441 aacggactgg cgctcggtgt catcaccgca ggctaacggg tttcatgcgg cagtgcagca
     4501 atggtttccg gcgtgaccat tggtgtcccg gggacaatcc cggagcgggc cgccctcagt
     4561 acgcgaactc gcggaacacc gggtccacgc tgccgttcca cgcgccatgg aacagggcga
     4621 gcttgcgctc ggccggggtt tcgccggcct cggcgatttc cagcaatggt gcgatgaagc
     4681 cggactcgtc gacgccgcag ctgtccgcgc gcgcgcggcg gcgcaggccg gcatcggcga
     4741 tcttcaatgc ctcgatcgcg atgtcgcgca gggtgccgcc gcggaacggg gtcttcagcg
     4801 cgtgcctggg cacgtcgtcg cgcagtgcgt tgcgctcggc catcgagaaa tccctgacca
     4861 ggtcccaggc cgcatccagc gcggcatcgt cgtacagcag gccgacccag aacgccggca
     4921 gcgcgcacag ccggttccac ggcccaccgt cggcaccgcg catctccagg aatttcttca
     4981 gccgcacttc cgggaaggcg gtggtcatgt ggtcgttcca gtcgcggatg gtcggcagcg
     5041 cgccgggcag cacgtccagt tcgccgcgca ggaacgtgcg gaacgacttg ccgctggcgt
     5101 cgatgtattc gccatcgcga tagctgaagt acatcggcac gtcgagcagg tagtcgacgt
     5161 agcgttcgta gccgaagccg tcctcgaaca cgaaatcgag catcccggtg cggtcgcggt
     5221 cggtgtcggt ccagatgtgc gagcggtagc tgaggtagcc gttcggcttg ccgtcggtga
     5281 acggcgagtc cgcgaacagc gcggtggcga ccggctgcaa cgccagcgac acccggaact
     5341 tcttcaccat gtccgcttcg ctggcgtaat ccaggttgac ctgcaccgtg caggtgcggg
     5401 tcatcatgtc gaggccgagc gtgccgacct tcggcatgta ctcgcgcatg atcctgtagc
     5461 ggcccttcgg catccacggc atctcgtcgc gccgccactt gggctggaaa cccatgccga
     5521 ggaagccaag cccgagttcg tcggcgacct cgcgcacttc gcgcaggtgg ctgccgactt
     5581 cccggcaggt gtcgtgcagg gtttccaccg gcgcgccgga caattcgagt tgcccggcgg
     5641 gctccagggt caccgaggcc aggcccttgg tcagcgcgat gacatggcca tgctcctcga
     5701 acggcgtcca gccgaaacgc gcgaggccct tcagcaacgc ctcgatcccg cgttcgccgt
     5761 cgaaggtggg cgggcgcagg tcgtcgaggc ggaacccgaa tttctcgtgc tcggtgccga
     5821 tccgccaggc ctcgcgcggc ttctcgccgg aggccagcac gtcgaccagt tgccgatggt
     5881 cggtgatcgg cgtgtcgttg gcgctgctgg gtccggacat ggcgggcttc cgcggggagt
     5941 cagccagcat agccgctgcc gccagccgcc ggcagcccgg cagccggcct tcagtgttcc
     6001 ggctgcatac gtattcctga gcacgcgcgt tgtcggccac cacccgcgcg ggtagcctcg
     6061 acccgcactc cctcccgcgg cccgcccatg cgcctcctgc tcgcctgctg cctgtggctg
     6121 atcacgttcg cggtacccgc ggcgaccatc gaacgggtgg agcctggcaa ctggtgggtc
     6181 ggcatgcagc acaaccgggt cgaattgctg gtccacggca atgggatcgg caccgcgacg
     6241 ccaaggctcg tacatgccgg cgtatcgatc gccggggtgc accggaccga caacccgaac
     6301 tacctgttcg tcaccgtcca gctcgcgggc gatgcggcgc caggcgtgtt cgacatcgaa
     6361 ttcgtcgaag ccggcaaggt cgtcgccacc catccgtggc ggctcgacgc gcgcgaaccc
     6421 ggttccgcgc aacgccgtgg cttcgacgcg cgcgacgcga tctacctggt cacgcccgac
     6481 cgcttcgcca acggcgatcc gtccaacgac agcgtcgccg gcatggccga ggcggccgat
     6541 cgcgcggacc cgaacggccg ccacggcggc gacatcgccg gcatccgcaa gcacctggac
     6601 tacatcgccg gcatgggctt cacccagctg tggcc
//
LOCUS       DAORUK010000115         6618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_13440_length_6618_cov_17.511047, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000115 DAORUK010000000
VERSION     DAORUK010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13440_length_6618_cov_17.511047"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PK818_08030"
     CDS             <1..217
                     /locus_tag="PK818_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008576699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HQY82714.1"
                     /translation="GVFLGQSTRVYNRATGEVSYGRVPPYSVVVSGQLPAADGSHSLY
                     CAVIVKQVDAKTRSKTSVNELLRGLAD"
     gene            298..2187
                     /locus_tag="PK818_08035"
     CDS             298..2187
                     /locus_tag="PK818_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HQY82715.1"
                     /translation="MKTRLACCLLAAAVTAASAAPAQDLSRYSDEGLRAELAGIADAE
                     TAVLVPMRDGKGLSTNIWRPKGASGKLPVVLWKTPYDEHVPAGSTARYALEAVRRGYA
                     FIVQNERGRYFSQGTYEILGRPQTDGYDTLSWIAAQPWSNGKVGTLGCSSSAEWQLAL
                     AGMNHPAHAAMVPMAAGAGIGKVGRFLEQGNFYTGGVPRTLFGVWLYGVDNPLRAELP
                     QDLNGPMRARVARYNSLDADKPDVDWKTHVRRLPYAELLSSLGEPPGTFESLIARGPA
                     DPAWKTGGLYHDDMGWGVPALWFNSWYDVSIGPNMALYNHARTVASDREASANQYVVV
                     GPNPHCQFARLSEDYKVGERAMGDARFPVDQQLWAFFDRWLKGKPAAFPDGTAHVRYF
                     TMGANRWQSDAEWPPKAARTLRMYLRSGGRANSMFGDGRLAADAPPAGEAKDSYRYDP
                     RNPVQTIGGGDCCNGGLVTAGAFDQRQISARNDVLVYDSEPLQAPMEVSGFVDAVLKV
                     SSSARDTDFAVKLVDVAPDGTAWIVGDTIQRARYRDSYTAPAPMQPGQVYTIRPTPIT
                     TSIQFGKGHRIRVEVTSSNFPKFARNLNTGGPNETESTPVVADNAIHHDADNASYIEL
                     PVVGG"
     gene            2253..2612
                     /locus_tag="PK818_08040"
     CDS             2253..2612
                     /locus_tag="PK818_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HQY82716.1"
                     /translation="MAATLYGLDNCDTCRKARNWLLRFGIEHRFVDYREQRQPPETLL
                     AWKDAVGGWDALVNKSSTTWRQLAPNRKAPGSDPEWKLLLKEYPQLVRRPVVVLDGGS
                     VSQGFSDNGFKKLFGLL"
     gene            2687..3817
                     /gene="dapE"
                     /locus_tag="PK818_08045"
     CDS             2687..3817
                     /gene="dapE"
                     /locus_tag="PK818_08045"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010366193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HQY82717.1"
                     /translation="MSDVLQLTRDLIARPSVTPDDAGCQQLVAERLGRAGFACERLRF
                     GAVDNLWATHGGGDGPVLVLLGHTDVVPPGPRDAWASDPFAPEVRDGVLFGRGAADMK
                     GSVAAFVVALERFVAVHPDHAGTVALLLTSDEEGDAVDGVRRVAELFRERGQRIDWCI
                     TGEPSSTSTLGDLLRVGRRGTLSATLTVKGIQGHVAYPEKARNPIHQSLAALAELAWK
                     HWDQGYESFPPTSLQISNIHAGTGANNVIPGELQVLFNLRYNPNWTAAQLEAECEALL
                     GEYGLDFSVQWQRGGEPFHTPEGPLRAAAREVLAGFAGAPPEESTGGGTSDARFIAPL
                     GAQCVEIGPVNASIHKVDEHIAVADLEALPDLYHALLERLLG"
     gene            complement(3824..4297)
                     /locus_tag="PK818_08050"
     CDS             complement(3824..4297)
                     /locus_tag="PK818_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HQY82718.1"
                     /translation="MTHDLDRTDRRLLAALQDDARLTVAELAERVALSTSPCWRRVRR
                     LEQAGYVTGYHAQLSAERLGYGITAFVSVVMGSHTQEVARAFEARLRDIPEVIACHNV
                     SGRYDFLLEVVATDLAAYGEFARNVLQALPGVTELHSSFSLKSLKAERRLPLHGT"
     gene            4423..5640
                     /locus_tag="PK818_08055"
     CDS             4423..5640
                     /locus_tag="PK818_08055"
                     /EC_number="4.4.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF005695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018826 - methionine gamma-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase"
                     /protein_id="HQY82719.1"
                     /translation="MGMPGFSTTAIHHGYDPRDHHGALVPPVYQSATFTFASAAGGGR
                     CFAGEADGYIYTRIANPTLALLESRLAVLEGGAAAIAFGSGMGAITATLWTLLRPGDE
                     IVVDLTLYGCTFAFLHHGIGEFGVRVTHVDMTDPSAVEAAMAGKPKVVYFESPANPNM
                     RLVDIEAVAAAAHRAGALVVVDNTYCTPYLQRPLALGADVVVHSATKYISGHGDVTAG
                     IAVAADAELAQRIRLQGLKDMTGAVLSPQDAALLMRGLKTLALRMDRHCTNAMAVARL
                     LQAHPAVAKVHYPGLEADPQHGLAKRQMRQPGGMIAFELQGGYEAGMRFIDALGMVAC
                     AVSLGDAESLAQHPASMTHSTYTPEQREAAGISDGLVRLSAGLEDTDDLVADVARALS
                     ACAAPKLRAIVAA"
     gene            5798..>6618
                     /locus_tag="PK818_08060"
     CDS             5798..>6618
                     /locus_tag="PK818_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HQY82720.1"
                     /translation="MCSILGVFDLRPGADLHPLRPLALSLSARQRHRGPDWSGVHVDA
                     NALLVHERLAIVDPAGGAQPIHSADGELALAVNGEIYNHRDLERRLARPYAFQTGSDC
                     EVINALHRDGEAPETWLGRLNGIFAFALWDAAAKRVLIARDHMGICPLYWGHDADGRL
                     WVASEMKSLVRVCDDVAAFPPGHAYDSATGELVHWYKRAWRDYDAVQGVAADKAELRE
                     AFERAVHRQLMSDVPYGVLLSGGLDSSLVAACAAKFARKRVEDDDKGEAWWPRLH"
BASE COUNT         1034 a         2275 c         2334 g          975 t
ORIGIN      
        1 gggcgtgttc ctgggccaga gcacgcgcgt ctacaaccgc gccaccggcg aggtctccta
       61 cggccgggtg ccgccctaca gcgtggtcgt ctccggccag ctgccggcgg ccgatggcag
      121 ccattcgctg tactgcgcgg tgatcgtcaa gcaggtcgac gcgaagacgc gaagcaagac
      181 gtcggtcaac gaattgctgc gcggcctggc ggattgaccc gctggcgtga cttccggcag
      241 gggtggcggt gcgcgcttcg tgccaaacag gaggcattgc ctgcccgggg acctgccatg
      301 aagacacgcc ttgcctgctg cctgctcgcc gccgccgtga ccgcggccag cgcggcgccg
      361 gcgcaggacc tttcgcgcta cagcgacgag ggcctgcgcg ccgagctcgc cgggatcgcc
      421 gatgcggaga ccgcggtcct ggtgccgatg cgcgacggca agggcctgtc caccaacatc
      481 tggcggccga agggggccag cggcaagctg ccggtggtgc tctggaagac cccctacgat
      541 gaacacgtgc cagcgggcag cactgcgcgc tatgcgctcg aggcggtgcg gcgcggctat
      601 gccttcatcg tccagaacga acgcggacgc tacttcagcc agggcaccta cgagatcctc
      661 ggacgtccgc agaccgacgg ctacgacacc ctcagctgga tcgccgcgca gccgtggtcg
      721 aacggcaagg tcggcaccct cggctgttcg tcctcggcgg agtggcagct ggcgctggcg
      781 gggatgaacc acccggcgca cgcggcgatg gtgccgatgg ccgcaggcgc ggggatcggc
      841 aaggtcggcc ggttccttga gcaggggaac ttctacacgg gcggggtccc gcgcaccctg
      901 ttcggggtgt ggttgtacgg cgtcgacaat ccgctgcggg cggaattgcc gcaggacctc
      961 aacggcccga tgcgcgcgcg cgtcgcccgc tacaacagcc tggatgcgga caagccggac
     1021 gtcgactgga agacccacgt gcgccgcctg ccgtatgcgg agctgctgtc ctcgctgggt
     1081 gagccgccgg ggacgttcga gtcgctgatc gcgcgcggcc ccgccgaccc cgcatggaag
     1141 accggcggcc tgtaccacga cgacatgggt tggggcgtgc cggcgctgtg gttcaacagc
     1201 tggtacgacg tctcgatcgg gccgaacatg gcgctgtaca accatgcgcg cacggtcgcc
     1261 agcgaccgcg aggcgagcgc gaaccagtac gtggtggtcg gtcccaaccc gcattgccag
     1321 ttcgcccgcc tcagcgagga ctacaaggtc ggcgagcgcg cgatgggcga cgcccggttc
     1381 ccggtcgacc agcagctgtg ggccttcttc gaccgctggc tgaagggcaa gccggcggcg
     1441 ttcccggacg gcaccgcgca cgtccgctac ttcacgatgg gcgccaaccg ctggcagtcc
     1501 gacgccgagt ggccgccgaa ggcggcgcgc acgctgcgga tgtacctgcg ctccggtggc
     1561 cgcgccaatt cgatgttcgg cgacggccgc ctggccgcgg acgcaccgcc tgcaggcgag
     1621 gcgaaggaca gctaccgcta cgacccgcgc aacccggtcc agaccatcgg cggcggcgac
     1681 tgctgcaacg gcgggctggt caccgccggc gcgttcgacc agcgccagat ctctgcgcgc
     1741 aacgacgtgc tggtctacga cagcgagccg ctgcaggcgc cgatggaagt cagcgggttc
     1801 gtcgatgcgg tgctgaaggt ctcgtcctcc gcgcgcgaca ccgacttcgc ggtgaagctg
     1861 gtggacgtgg cccccgacgg caccgcgtgg atcgtgggcg acaccatcca gcgcgcgcgc
     1921 tatcgcgaca gttacaccgc gcccgcgccg atgcagccgg gccaggtgta cacgatccgg
     1981 ccaaccccga tcaccacctc gatccagttc ggcaagggcc accgcatccg ggtcgaggtg
     2041 accagctcga acttcccgaa gttcgcgcgc aacctcaaca ccggtggccc gaacgagacc
     2101 gagtcgacgc cggtggtcgc cgacaacgcg atccaccacg acgccgacaa cgccagctac
     2161 atcgagctgc cggtggtcgg cggctgagcc gcggctcgcc gccggccatg gctttcggca
     2221 tcatgcccgc actccacgca acggatacgg gcatggccgc caccctgtac gggctcgaca
     2281 attgcgacac ctgccggaag gcgcgcaact ggctgctgcg cttcggcatc gagcaccgtt
     2341 tcgtggatta ccgcgagcag cggcagccgc cggaaacgct gctggcctgg aaggatgcgg
     2401 tgggcggctg ggatgcactg gtcaacaagt cctccacgac ctggcgccag ctggcgccga
     2461 accgcaaggc gccgggcagc gatcccgaat ggaagttgct gctgaaggaa tatccgcaac
     2521 tggtgcgacg gccggtggtg gtgctggacg gcggcagcgt gtcgcagggg ttcagtgaca
     2581 acggattcaa gaagctgttc ggactcctgt aggagcggcg caagccgcga ccaatagtca
     2641 cgaacaacca catcgcggcc tgcgccgcgc ctacgacggg caacccatga gcgacgttct
     2701 ccaactcacc cgcgacctga tcgcgcggcc ctcggtcacc cccgacgatg ccggctgcca
     2761 gcaactggtc gcggagcgcc tggggcgcgc cggtttcgcc tgcgaacgcc tgcgcttcgg
     2821 cgcggtcgac aacctgtggg cgacgcatgg cggtggcgat ggtcctgtgc tggtgctgct
     2881 gggccacacc gacgtggtgc ccccgggccc gcgcgatgcc tgggccagcg atccgttcgc
     2941 gccggaggtg cgcgacggcg tgctgttcgg ccgcggcgcg gcggacatga agggcagcgt
     3001 ggcggcattc gtggtggcgc tggagcggtt cgtggccgtg catccggacc atgccggcac
     3061 cgtcgcgttg ctgctgacct cggacgagga gggcgatgcc gtcgatggcg tgcgccgggt
     3121 cgccgagctg ttccgcgaac gcggccagcg gatcgactgg tgcatcaccg gcgagccgtc
     3181 atcgacatcg acgcttggtg acctgctgcg cgtcggccgc cgcggcacgc tgtctgcgac
     3241 gctgacggtg aagggcatcc agggccacgt ggcctatccg gaaaaggcgc gcaacccgat
     3301 ccaccagtcg ctggcggcgc tggccgagct cgcctggaag cattgggacc agggctacga
     3361 gagcttcccg ccgacaagcc tgcagatcag caacatccac gcaggcaccg gcgccaacaa
     3421 cgtgatcccc ggcgagctgc aggtgctgtt caacctgcgc tacaacccca actggaccgc
     3481 tgcgcaactg gaggcggaat gcgaggccct gctcggcgag tacgggctgg acttcagcgt
     3541 gcagtggcag cgcggcggtg aaccgttcca cacccccgag ggcccgttgc gcgccgccgc
     3601 gcgcgaggtg ctggccgggt tcgccggggc gccgccggag gaaagcaccg gcggcggcac
     3661 ctcggatgcg cgcttcatcg ccccgctcgg cgcgcagtgc gtggagatcg ggccggtcaa
     3721 cgcgagcatc cacaaggtcg acgaacacat cgcggtagcc gacctcgagg ccttgcccga
     3781 cctctaccac gcgttgctgg agcggttgct gggctgagcc cgctcaggtg ccgtgcagcg
     3841 gcagccggcg ttccgccttg agcgacttca gcgagaagct ggagtgcagt tcggtcacgc
     3901 cgggcaaggc ctgcagcacg ttgcgcgcga actcgccgta ggcggcaagg tcggtggcga
     3961 ccacttccag caggaagtcg tagcgcccgg acacgttgtg gcaggcgatc acttccggga
     4021 tgtcgcgcag gcgcgcctcg aaggcgcgcg ccacctcctg ggtgtggctg cccatcacca
     4081 cgctgacgaa ggcggtgatc ccgtaaccca gccgctccgc cgacagttgc gcgtggtaac
     4141 cggtcacgta gccggcctgc tccagccggc gcacccgccg ccagcatggc gaggtcgaca
     4201 gcgccacccg ttcggcgagt tcggccacgg tcaggcgcgc gtcgtcctgc agcgcggcca
     4261 gcaggcggcg gtcggtgcgg tccaggtcat gggtcatgga aggatcaacc tcgtttatct
     4321 tcaagttggg aacaatttac cccggttgtg ggtgaatggc tgcatcttcg gaaaatgctt
     4381 tcgccagggg aggcatacgc tatccgcatc gaatagcgga gcatgggcat gcccggtttc
     4441 tccaccaccg ccatccacca tggctacgac ccgcgcgacc accacggcgc gctggtgccg
     4501 ccggtgtacc agagcgccac cttcactttc gccagcgcgg ccgggggcgg gcgctgcttc
     4561 gccggcgagg cggacggcta catctacacg cgcatcgcca acccgaccct ggccctgctg
     4621 gagtcgcggc tggcggtgct ggaaggcggc gccgccgcga tcgcgttcgg ctccggcatg
     4681 ggcgcgatca ccgccaccct gtggaccctg ctgcgccccg gcgacgagat cgtggtcgac
     4741 ctcaccctgt acggctgcac cttcgcgttc ctgcaccacg ggatcggcga gttcggggtg
     4801 cgcgtgaccc atgtcgacat gaccgacccg tcggcggtcg aggccgcgat ggccggcaag
     4861 cccaaggtcg tgtacttcga aagcccggcc aatcccaaca tgcggctggt ggacatcgaa
     4921 gcggtcgcgg ccgcggcgca tcgcgccggt gcgctggtgg tggtcgacaa cacctactgc
     4981 accccgtacc tgcagcgccc gctcgcgctg ggtgcggacg tcgtggtgca ttcggcgacc
     5041 aagtacatca gcggccatgg cgacgtcacc gccggcatcg cggtggccgc ggacgccgag
     5101 ctggcccagc gcatccgcct gcagggcctg aaggacatga ccggcgcggt gctctcgccg
     5161 caggacgccg cgctgctgat gcgcgggctg aagacgctgg cgctgcggat ggaccgccac
     5221 tgcaccaacg cgatggcggt ggcgaggctg ctgcaggcgc atccggcggt ggcgaaggtg
     5281 cattaccccg gcctggaagc cgacccccag cacggattgg cgaagcggca gatgcgccag
     5341 cccggcggga tgatcgcgtt cgagctgcag ggcggctacg aggcgggcat gcgcttcatc
     5401 gacgcgctcg ggatggtggc ctgtgcggtc agcctgggcg atgccgaatc gctggcgcag
     5461 caccccgcca gcatgaccca ctcgacctac acgccggaac aacgcgaggc cgcgggcatc
     5521 tccgacggac tggtgcggct gtcggcgggg ctggaggata ccgacgacct ggtggccgac
     5581 gtcgcgcgcg cgctgtcggc ttgcgcggcg ccgaagttgc gtgcaatagt cgccgcatga
     5641 gcctgcagcg cgccagcaac aataatcgcc cgaacgccag ccccccgcgg gtcggcgatt
     5701 agcgcgcgca tcacacgctg atcttcccga ggcccgcact gcaaagtgcg ggcctcttcg
     5761 ttttcccctc gaaaaacacc ccacaaggac ttccacgatg tgctcgatcc tcggcgtctt
     5821 cgacctccgc cccggcgcgg acctgcatcc cctgcggcca ttggcgctgt ccctgtccgc
     5881 gcgccagcgc caccgcgggc cggactggag cggggtgcac gtggatgcga acgcgctgct
     5941 ggtccatgaa cggctggcga tcgtcgatcc cgccggcggc gcgcagccca tccattccgc
     6001 ggacggcgaa ctggcgctgg cggtcaacgg cgagatctac aaccatcgcg acctcgagcg
     6061 ccggctggca aggccctatg ccttccagac cggttccgac tgcgaggtga tcaacgcgtt
     6121 gcatcgcgat ggcgaagcgc ccgagacctg gctgggccgg ctcaacggca tcttcgcctt
     6181 tgcgttgtgg gatgcggccg cgaagcgcgt gttgattgcg cgcgaccaca tgggcatctg
     6241 tccgctgtac tggggccacg acgccgatgg ccgcctgtgg gtggcctcgg agatgaagtc
     6301 gctggtgcgc gtctgcgacg acgtcgccgc gtttccgccc ggccatgcct acgacagcgc
     6361 caccggcgag ctggtgcatt ggtacaagcg tgcgtggcgc gactacgacg cggtgcaggg
     6421 cgtggctgcg gacaaggccg aactgcgcga ggccttcgag cgcgcggtgc atcgccagct
     6481 gatgagcgac gtgccgtacg gcgtgctgct gtcgggcggg ctggattctt cgctggttgc
     6541 ggcctgcgcg gccaagttcg cgcgcaagcg ggtggaggac gatgacaagg gcgaggcctg
     6601 gtggccgcgc ctgcacag
//
LOCUS       DAORUK010000116         6609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_13464_length_6609_cov_16.949954, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000116 DAORUK010000000
VERSION     DAORUK010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13464_length_6609_cov_16.949954"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..787)
                     /gene="lolA"
                     /locus_tag="PK818_08065"
     CDS             complement(170..787)
                     /gene="lolA"
                     /locus_tag="PK818_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017158314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HQY82721.1"
                     /translation="MKPATSILAAALLLAATAANAGGRDQLDTFTKGLKGLDGQFSQQ
                     VFDARGKQKEASTGRVALSAPRLFRWEYVKPYPQLIVADGTTVWVHDPDLQQVSKRPQ
                     GVEEANSPLAILLDPSRLDREFTVQDAGTVAGVEWLQLTPKQATAPFKSARLGFGKAG
                     LVQMEYIDALGQRTTISFSGWKRNPTFAKGTFVYRPGKGVDVIGG"
     gene            complement(1004..3358)
                     /locus_tag="PK818_08070"
     CDS             complement(1004..3358)
                     /locus_tag="PK818_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HQY82722.1"
                     /translation="MGKAGRKPASRASKPAMSPGRQRLWRDLALIAVAPLLLFLLACL
                     VTYSPADPGFSSSGSITAPVHNACGRVGAWIADVLLMLTGYVAFLLPVVLGAIAWIAL
                     FGMDSDGDGDADLGPALRLVGIVGFLVSATGLLALRVAGSHDLPEGSGGVVGQLIGNS
                     LGMLFGALGANLFLLSLFLISVTLATGLSWLAVMDRIGGWVLALPGLLSRGARKSAQQ
                     ASEWQETRALREERGEARKVDTELRAKRAPVKIEPPAAPVVEKSDRAKRETQIPLFQG
                     GDGSGIPPLALLDDPKPQPKGYSEETLETLSRQIEFKLKDFRIDAEVVGAYPGPVITR
                     FEVQPAAGVKVSQISSLDKDIARGLSVKSVRVVDVIPGKSVIGLETPNTHREMIYLSE
                     LLRSKEYDKSNSPLTLALGKDIAGRPTVADLARMPHLLVAGTTGSGKSVAVNAMVLSL
                     LYKASAKDLRMLMIDPKMLELSVYQGIPHLLAPVVTDMKEAANGLRWCVAEMERRYKL
                     MSAVGVRNLAGFNKKVKDAQDSGQPMMDPLFKPNPDLDEVPRQLETLPFIVIFIDEFA
                     DMMMIVGKKVEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRIAFQVSS
                     KIDSRTILDQSGAETLLGHGDMLYLPPGTAMPERVHGAFVSDEEVHRVVDHLKQSGGG
                     PDYIEGVLEEAQTMYDGSSVGATGLPETASGSDDPEADPLYDQAVQIVTETRRASISG
                     VQRRLKIGYNRAARMIEAMEAAGIVSTPEHNGDRSVLAPPPPRP"
     gene            3560..4513
                     /gene="trxB"
                     /locus_tag="PK818_08075"
     CDS             3560..4513
                     /gene="trxB"
                     /locus_tag="PK818_08075"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HQY82723.1"
                     /translation="MTPTKHTRLLILGSGPAGWTAAVYAARANLKPVVVTGMAMGGQL
                     MTTTEVDNWPGDAHGLLGPDLMARMQAHAERFDTDVVFDHIHSADLSQRPFRLKGDSG
                     DYTCDALVIATGATAKYLGIPSEEAFKGRGVSACATCDGFFYKDQDVAVIGGGNTAVE
                     EALYLSNIARKVYLVHRRDTLRAEKIMQDKLFAKIASGKIEAVWHHAVDEVLGNEAGV
                     TGMRVKSVLDGSTREIAVQGFFVAIGHAPNTALFDGQLEMKNGYLKIQTGLDGNATQT
                     SVPGVFAAGDVADQVYRQAITSAGFGCMAALDAEKFLDQHD"
     gene            4510..5664
                     /locus_tag="PK818_08080"
     CDS             4510..5664
                     /locus_tag="PK818_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY82724.1"
                     /translation="MMAAARLHRALSEIPADAWDALHDGRNPFVAHAFLAGLEAHGCL
                     REAWGWTPCHLGLWDGDVLVAAAPGYLKSNSHGEFVFDHAWAQAHAQHGLEYFPKWLG
                     AVPYSPVTGPRLLALEDGARRDLVAAIAGMVRASGLSSAHVNFHDGPDEAAFAHGQSP
                     DDRNWSLREDIQYHWHNEAGWTGFDAFLAAMDHKHRKNIRQERRKLREAGIDFRIVHG
                     DEASDADLAAMHGFYLATFADYGNSPALTLDFLRHLAREMPRSLVIVLGQRDGAPVAG
                     ALCLRGGDTLYGRYWGASELHPGLHFETCYYQGIEYCLREGLARFEPGAQGHHKIARG
                     FLPTLVRSRHWIADPRFARAIGAWCMEEREAVRAHLQQSLAHSPFRAGPA"
     gene            5661..6410
                     /gene="aat"
                     /locus_tag="PK818_08085"
     CDS             5661..6410
                     /gene="aat"
                     /locus_tag="PK818_08085"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006448846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HQY82725.1"
                     /translation="MSLRLLQVDNDPGAPFPPVDQALAEPDGLLAFGGDLSPQRLLNA
                     YRHGIFPWFSDGEPILWWSPSRRAVFRTDGVHLSSRFRRGLRTSRLLVRADTAFDAVI
                     GACAHNPRPGQSGGTWITRGMIQAYARLHRLGHAHSIEVWDGEHLVGGLYGLAIGRMF
                     CGESMFSAQSGASSVALAALARRLHGWGWPLIDAQVPNDHTRRLGVETWSRGAYLQAL
                     AGLRDAPDAVEAWSGRFGDIAAAELAHPAAA"
     gene            6510..>6609
                     /locus_tag="PK818_08090"
     CDS             6510..>6609
                     /locus_tag="PK818_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HQY82726.1"
                     /translation="MAKDDVIEFEGTISETLPNTMFRVKLENGHEII"
BASE COUNT         1007 a         2295 c         2226 g         1081 t
ORIGIN      
        1 gtctgcttgc ttgggtggtg cgggcatccc gcgattctac gcgggcatcg tcaagacgat
       61 ttcaggcagc cacccatggc gcgacaccgg cggcatgcga gcccctgccc ggccatccaa
      121 gggcgggcgt tcgacgagcc gcgcgccagt ggcgcgcaag cggctcgttt cacccgccga
      181 tcacgtccac gcccttgccc ggccggtaga cgaaggtgcc cttcgcgaag gtcgggttgc
      241 gcttccagcc gctgaaggaa atcgtggtgc gctggcccag cgcatcgatg tattccatct
      301 gcaccagccc ggccttgcca aagccgagcc gcgcggactt gaacggcgcc gtggcctgct
      361 tcggggtgag ctgcagccat tccacgccag cgaccgtgcc ggcatcctgg accgtgaatt
      421 cgcggtcgag cctggacgga tccagcagga tcgccagcgg gctgttggct tcctccacgc
      481 cctgcgggcg cttgctgacc tgctgcaggt cggggtcatg cacccaaacg gtggtgccgt
      541 cggccacgat cagctgcgga tacggcttga cgtattccca gcggaacagc cgcggcgcgg
      601 acaacgccac ccgtccggtc gaagcttcct tctgcttgcc gcgggcgtcg aacacctgct
      661 ggctgaactg cccgtcgagg cccttcaggc ccttggtgaa ggtgtcgagc tggtcgcgcc
      721 cgccggcatt ggcggcagtc gcggccagca gcagtgcggc ggccaggatg gatgtcgcag
      781 gcttcatggg gagtctcctt cggtaggcgc ggcgtcggca caggcggtag gcggcccttg
      841 ccacgaaatc aaggaggagg cagtggccgc aaggccagtg ccgcgggacg ttcgcggcaa
      901 gggccgcgta ccgcccgtgc cgcgaccttg cgatgcagtg tgtgcgaaga aacctgaatg
      961 cgactgcacg caaaccgagc ggggctgaat cgcctgctcg tggtcaggga cgcggcggcg
     1021 gcggtgccag cacggagcgg tcgccattgt gttccggcgt gctgacgatg cccgccgctt
     1081 ccatcgcctc gatcatgcgc gcggcgcggt tgtagccgat cttgagtcgc cgctgcacgc
     1141 cggagatcga ggcgcggcgg gtttcggtga cgatctgcac ggcctggtcg tacagcgggt
     1201 cggcttccgg atcgtcgctg ccggatgcgg tctccggcag cccggtggca cccacgctgc
     1261 tgccgtcgta catcgtctgc gcttcttcca gcacgccttc gatgtagtcc ggccctcccc
     1321 cgctctgctt gagatggtcg accacccgat gcacttcctc gtcgctgacg aaggcgccat
     1381 gcacgcgctc cggcatcgcg gtgccgggcg gaaggtacag catgtcgccg tggcccagca
     1441 gtgtttccgc gccgctctgg tcgaggatgg tgcggctgtc gatcttgctg ctcacctgga
     1501 acgcgatgcg ggtcgggatg ttggccttga tcaggccggt gatcacgtcc accgacgggc
     1561 gctgggtggc caggatcagg tggatgccgg ccgcacgtgc cttctgcgcc agccgcgcga
     1621 tgagttcctc gaccttcttg ccgacgatca tcatcatgtc ggcgaattcg tcgatgaaga
     1681 tgacgatgaa cggcagcgtt tccaactggc gcggcacttc gtccaggtcg gggttcggct
     1741 tgaacagcgg atccatcatc ggctgcccgc tgtcctgcgc gtccttcacc ttcttgttga
     1801 agccggccag gttgcgcacg ccgaccgcgc tcatcagctt gtagcggcgc tccatttccg
     1861 ccacgcacca gcgcaggccg ttggcggcct ccttcatgtc ggtgaccacc ggcgccagca
     1921 ggtgcgggat gccctggtag acgctgagct ccagcatctt cgggtcgatc atcagcatcc
     1981 gcaggtcctt cgcggatgcc ttgtacagca ggctcaacac catcgcgttg accgcgaccg
     2041 acttgcccga accggtagtg ccggcgacca gcaggtgcgg catccgcgcc aggtcggcca
     2101 ccgtcggccg gccggcgatg tccttgccca gcgccagcgt cagcgggctg ttggacttgt
     2161 cgtattcctt ggagcgcagc agttcgctca ggtagatcat ctcgcggtgg gtgttcggcg
     2221 tttccaggcc gatcaccgac ttgcccggga tcacgtcgac cacccgcacc gacttcaccg
     2281 acagcccgcg cgcgatgtcc ttgtccagcg aggaaatctg gctgaccttg acccccgccg
     2341 ccggctgtac ctcgaagcgg gtgatcacag ggcccggata ggcacccacc acttccgcgt
     2401 cgatgcggaa gtccttgagc ttgaattcga tctgccgcga cagcgtttcc agggtctcct
     2461 cgctgtagcc cttgggctgc ggcttgggat cgtccagcag cgccagcggc gggatgccgg
     2521 agccgtcgcc gccttggaac agcgggatct gggtctcgcg cttggcgcgg tcgctcttct
     2581 cgaccaccgg cgcggccggc ggctcgatct tcaccggtgc acgcttcgcg cgcagctcgg
     2641 tatcgacctt gcgcgcttcc ccgcgttcct cgcgcagcgc ccgggtttcc tgccattcgc
     2701 tggcctgctg cgcactcttg cgcgcgccgc ggctgagcag gccgggcagc gccagcaccc
     2761 agccgccgat ccggtccatc accgccagcc acgacaaccc ggtggccagg gtcaccgaga
     2821 tcaggaacag gctgagcagg aacaggttgg cgccgagcgc gccgaacagc atgcccagcg
     2881 agttgccgat cagctgcccc accacaccgc cgctgccttc gggcaaatca tgggaacccg
     2941 ctacacgaag ggccagcagg ccggtggcgg agacaaggaa cccaacgatg ccgaccaggc
     3001 gcagcgccgg accgaggtcg gcatcgccat cgccatcgct gtccatcccg aacagcgcga
     3061 tccaggcgat cgcaccgagc accaccggca gcaggaacgc cacgtagccg gtcagcatca
     3121 gcagcacgtc ggcaatccac gcaccgacgc ggccacaggc gttgtggacc ggcgcggtga
     3181 tgctgccgga cgacgagaac ccgggatcgg ccggcgaata ggtgaccagg caggcgagca
     3241 ggaacagcag cagcggcgcc accgcgatca gcgcgaggtc gcgccacagc cgctggcgcc
     3301 ccgggctcat cgccggcttg ctggcccggg acgcaggctt gcgccctgcc ttgcccattt
     3361 cgggagccgt gcgtgccacc tgtgatcaga cctcaggaaa ttcccctagt ggtttgatat
     3421 tacgacattt gtcgccttcg gcacagcgcg tgcgggcggc caggtggcgg gcagcgttgc
     3481 ggacatcgcg ccttgtgctg gcgctggccc gcctccaagc tatggaactc ccgcgacccg
     3541 tcgccccaag cctgcactca tgaccccgac caagcacacc cgcctgttga tcctcggttc
     3601 cggccccgcc ggctggaccg ccgccgtgta cgcggcgcgc gccaacctca agccggtggt
     3661 cgtcaccggc atggccatgg gcggccagct gatgaccacc accgaggtcg acaactggcc
     3721 gggcgatgcc cacggcctgc tgggcccgga cctgatggcg cggatgcagg cgcatgccga
     3781 gcgcttcgac accgacgtgg tgttcgacca catccacagc gccgacctct cgcagcggcc
     3841 gttccggctc aagggcgatt ccggcgacta cacctgcgat gcgctggtga tcgccaccgg
     3901 tgccaccgcc aagtacctcg gcatcccgtc cgaggaagcg ttcaagggcc gcggcgtgtc
     3961 cgcctgcgcc acctgcgacg gcttcttcta caaggaccag gacgtggccg tgatcggcgg
     4021 cggcaacacc gccgtggaag aggccctgta cctctccaac atcgcccgca aggtctacct
     4081 ggtgcaccgc cgcgacacct tgcgcgcgga gaagatcatg caggacaagc tgttcgcgaa
     4141 gatcgcgtcc ggcaagatcg aggcggtctg gcaccacgcg gtggacgaag tgctgggcaa
     4201 tgaagccggc gtcaccggca tgcgcgtgaa gtcggtgctc gacggcagta cccgcgagat
     4261 cgcggtccag ggcttcttcg tcgcgatcgg gcacgcgccc aacaccgcgc tgttcgacgg
     4321 ccagctggag atgaagaacg gctacctgaa gatccagacc gggctggacg gcaacgccac
     4381 ccagaccagc gtccccggcg tgttcgccgc cggcgacgtc gccgaccagg tgtaccggca
     4441 ggcgatcacc tccgccggct tcggctgcat ggcggcgctg gatgcggaga agttcctgga
     4501 ccagcacgac tgatggcggc tgcgcgcctg caccgcgcgc tgtccgaaat ccccgccgat
     4561 gcctgggatg cgctgcacga tggccgcaac cccttcgtcg cccacgcctt cctggccggg
     4621 ctggaagcgc acggctgcct gcgcgaggcg tggggctgga caccgtgcca cctgggcctg
     4681 tgggacggcg atgtgctggt cgcggccgcg cccggctacc tgaagtccaa ctcccacggc
     4741 gaattcgtgt tcgaccatgc gtgggcgcag gcgcatgcgc agcatggtct cgaatacttc
     4801 ccgaaatggc tgggcgcggt gccctactcc ccggtcaccg ggccgcggct gctggccctg
     4861 gaggatggcg caaggcgcga cctggtcgcg gcgatcgcag gcatggtgcg tgcgtccggg
     4921 ttgtcgtcgg cgcacgtcaa cttccacgat ggaccggacg aggcggcgtt cgcccacgga
     4981 cagtccccgg atgaccggaa ctggtcgctg cgcgaagaca tccagtacca ctggcacaac
     5041 gaagcgggct ggaccggctt cgacgccttc ctggcggcga tggaccacaa gcaccgcaag
     5101 aacatccgcc aggagcggcg caagctgcgc gaggccggca tcgatttccg catcgtgcac
     5161 ggcgacgagg ccagcgacgc ggaccttgcc gcgatgcatg gcttctacct ggccaccttc
     5221 gccgactacg gcaattcgcc cgcgctgacc ctcgatttcc tccgccacct ggcgcgcgag
     5281 atgccccgtt cgctggtgat cgtgctgggc cagcgggatg gcgcgcctgt cgcgggcgcg
     5341 ctgtgcctgc gcggcggcga cacgctctac gggcgctact ggggcgcgtc cgagctacat
     5401 cccggcctgc atttcgagac ctgctactac cagggcatcg agtactgcct gcgcgaaggc
     5461 ctggcccgct tcgagccggg ggcacagggc caccacaaga tcgcgcgcgg cttcctgccc
     5521 actttggtgc gcagccggca ctggatcgcg gatccacgct tcgcccgggc catcggcgca
     5581 tggtgcatgg aggaacgcga ggccgtccgc gcccacctgc agcagtcgct ggcgcattcc
     5641 cccttccgcg caggcccggc atgagcctgc gcctgttgca ggtggacaac gatcccgggg
     5701 cgccgttccc gccggtggac caggcgcttg ccgaaccgga tggcctgctc gccttcggtg
     5761 gcgacctgtc gccgcagcgc ctgctcaacg cctaccggca cggcatcttc ccgtggttct
     5821 cggatggcga gccgatcctg tggtggagtc cgtcgcggcg cgcggtgttc cggaccgatg
     5881 gcgtgcacct gtcgtcgcgg ttccggcgcg ggttgcggac ctcgcgactg ctggtgcgcg
     5941 cggataccgc gttcgatgcg gtgatcggtg cctgcgcgca caacccgcgc ccgggccagt
     6001 ccggcgggac gtggatcacc cgggggatga tccaggcgta cgcacggctg catcggcttg
     6061 gccatgcgca cagcatcgag gtgtgggatg gcgaacacct cgtcggcggc ctgtacggcc
     6121 ttgcgatcgg tcggatgttc tgcggcgaaa gcatgttctc cgcgcaatcc ggcgcatcca
     6181 gcgtggccct ggcggccctc gcccgccggc tgcacgggtg gggctggccg ctgatcgacg
     6241 cgcaggtgcc gaacgaccat acgcgtcggc tgggcgtgga aacctggtcg cggggcgcgt
     6301 acctgcaggc gctggcgggc ttgcgcgacg caccggacgc ggtggaagcg tggagcggac
     6361 gcttcggcga catcgcggcg gcggagctcg cccacccggc ggcggcttaa cgggtttttt
     6421 gcaagccctc gccacgcgtg tcagaatgcc gcgcttcggg tcgcggtcgc ggcccggctt
     6481 cacgtttcaa tccgggaata ccagccacag tggcaaaaga cgacgtcatc gaatttgaag
     6541 gcacgatctc cgagaccctc ccgaacacca tgttccgggt caagctggag aacggccacg
     6601 agatcatcg
//
LOCUS       DAORUK010000117         6597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_13503_length_6597_cov_18.514827, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000117 DAORUK010000000
VERSION     DAORUK010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13503_length_6597_cov_18.514827"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2115
                     /locus_tag="PK818_08095"
     CDS             <1..2115
                     /locus_tag="PK818_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY82727.1"
                     /translation="SIVVLESIAKARERGLGILEAAITGTREVSMAVVASTLTTIAVF
                     LPLVFVQGIAGQLFRDQALTVAIAIAVSLVVAMTLIPMLSALKGRPPMGFPEEPGHPR
                     WVPGKAWQKPAMYAGRGLGGLFRHGFFAVAWAITRLWRGIAALVGPVMRKASDLAMAP
                     YARAERTYLRILPNALARPTPVLLVASLAFLLTMAAVPLLGTDLIPQLAQDRFEMTAK
                     LPPGTPLAQTDALIREVQGKHAKDDGVRVLYGVSGTGTRLDASPTESGENIGKLSVVM
                     ADNDAEARLTDALRATMQANPSAQVDFSRPELFSLSPPLEIEVEGNDLESIRAAGAKL
                     AGMLRGNPHYADVKSTVEQGFPEIQIRFDQERAAALGLTTRQIADVVVNKVRGNVATR
                     YSFRDRKIDVLVRVQESDRASVEDIRRLIVNPGRGAPVELASVAEVVSTTGPSEIHRV
                     DQRRVAIVSANLRDVDLGTAISEVESMVGANPLGADIDMRIGGQGQELDESIRSLLFA
                     FALAIFLVYLVMASQFESLLHPFVILFTIPLALVGAVAALLLTRSPASVVVFIGLILL
                     VGLVVKNAIILIDKVNQLREAGVAKRAALVEGARSRLRPIIMTTMTALFGFMPLALAF
                     GEGSEVRSPMAITVIGGLLVSTLLTLVVIPIVYDLLDRKPDEAYAERGRSARRDAQAV
                     AEVLSHEHDPLGHGGADDGAKA"
     gene            2123..5203
                     /locus_tag="PK818_08100"
     CDS             2123..5203
                     /locus_tag="PK818_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY82728.1"
                     /translation="MNITELSIRRPVTTIMIFVSLVVIGLIAAFRLPLESQPEVSPPF
                     FFVQLPYPGSTPEEVERNLVRPVEEALATMPGIKSMNANATADGGGVFVQFSDWSRDV
                     AIAASEARERIDVVRDELPDDFRRYFVRRWSTGDQEAISLRLTSDDDLTARGDLIERS
                     LKQRLERLPGVARVEVEGVPTEEVLIAIEPDRLGANGVGLNELVARLQASNFSVSAGQ
                     ITDGGRRLQVQPKGELVDLQALRDLPIAANGTRLSDIAEVVMQPQRMNYVRQIDGKPA
                     VGVDIYKERAANLVELSAAVRREVQALQRDPALRGIAIEVTDDQGKEVTSSLIALAEA
                     GGIGLLLSIAVLFFFLRHWPSTLMVTTAIPICFVMTLGFMYFVGITLNILSMMGLLLA
                     VGMLVDNAVVVVESIYQEREKYPDQPRLASVIGTRHVAIALSAGTLCHCIVFLPMMFG
                     ETNMITIYLTQLAVTISVSLLASWLVAISLIPMLSARMQTPPAVKSGLISRLQERYAA
                     VLRWTLGHRGWSVLGIVLISLLSIIPMKMVKGDSGDEEDPSEIAIFYQWRGSYSKEEM
                     GKEVARVEAFLEANRKRFQVEQVFSRYSEQGWAQTRIDIGVNDPAESKQIQEALRESL
                     PKSARANIGIGRQGGLGASDKGIQFSLTGDSTETLQELSADIIPILARGKHLRDVRVD
                     TGDSNSELSVRVNRERAAAFGFSADQVAQFIGMALRGSSLREFQRGDVEIPVNVRFAG
                     AEHFGAADLESFTVRATDGRTVPLLAMVDVSTRPASTTIQRMNRQTMLKVEAGLASGA
                     TMPEAKAEIEATLGNVQFPPGYGYSFEGGGFGFNMDANAQMFRNIGIALVLMFIVMAA
                     VFESLLFPLAIMSCVLFSVFGVYWLFWLTGTEFNIMAIIGILVLMGVVVNNGIVMVEH
                     INNLRRRGLGRTDALVEGSRERLRPIMMTMGTAILAMVPIALSDTQIAGEGPAYYPMA
                     RAIAGGLAFSTVVSLLFLPTIYAMLDDLRAGSANLIRKARSTRAQRRALAAAAS"
     gene            complement(5274..6485)
                     /locus_tag="PK818_08105"
     CDS             complement(5274..6485)
                     /locus_tag="PK818_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HQY82729.1"
                     /translation="MTESTLPANTGFAGQPIVEIERDGVHYTLLGTAHVSKASIDAVL
                     AAIDSGRFDAVAVELDEQRHKALTNPDALAQLDLVKVIREGKVAPFAANLALAAYQRR
                     LAEQLGIEPGAELKAAAVEAAARNLPLQLIDRDVGITFRRILQGLSWWDRTKLIAGVA
                     GGLFDREEVSEQDIERLKEGDMLESSFGEFARDTPSLYATLIDERDQYMAAKLRERAD
                     GASHVLAVVGAGHLKGMATYLADEQRAPAELTGALSHVRRKRNIPWITLALMLLICGG
                     IAWGYAQGGADLGKDLLLQWVAWTAGLAGLGALLARGHPLSILAAAVSAPLKPFRPGV
                     PPGMFSALAEVHLRKPAYPDFLALRDDTQTLGGWYRNRVCRAVLVFLLTNLGSMVGVW
                     ISGAAIIGKLV"
     gene            complement(6478..>6597)
                     /locus_tag="PK818_08110"
     CDS             complement(6478..>6597)
                     /locus_tag="PK818_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQY82730.1"
                     /translation="TLLLAEVDMQRSEHVRRIWPFLRDRRIDAYADLTKRYRD"
BASE COUNT         1004 a         2216 c         2328 g         1049 t
ORIGIN      
        1 tcgatcgtgg tgctggagtc gatcgccaag gcgcgcgagc gcgggctcgg catcctcgag
       61 gcggcgatta ccggcacccg cgaggtcagc atggcggtgg tggcctccac cctcaccacc
      121 atcgcggtgt tcctgccgct ggtgttcgtg cagggcatcg ccggccagtt gttccgcgac
      181 caggcgctga cggtggcgat cgccattgcg gtgtcgctgg tggtggcgat gaccctgatc
      241 ccgatgctga gcgcgctcaa ggggcgtccg ccgatgggat tcccggagga gcccgggcat
      301 ccgcgctggg tgcccggcaa ggcctggcag aagccggcga tgtacgccgg gcgcggcctc
      361 ggcgggttgt tccggcacgg cttcttcgcc gtggcctggg cgatcacccg cctctggcgc
      421 gggatcgcgg cgctcgtcgg cccggtcatg cgcaaggcca gcgacctggc gatggcgccg
      481 tatgcgcgcg ccgagcgcac ctacctgcgg atcctcccga atgcgctggc gcggccaacc
      541 ccggtgctgc tggtcgccag cctggcgttc ctgctgacga tggcggcggt gccgctgctg
      601 ggcaccgacc tgatcccgca actggcgcag gaccggttcg agatgaccgc caagctgccg
      661 cccggcactc cgctggcgca gaccgacgcg ctgatccgcg aggtccaggg caagcacgcc
      721 aaggacgacg gcgtgcgcgt gctgtacggg gtgtccggca ccggcacccg gctcgacgcc
      781 agcccgaccg agagcggcga gaacatcggc aagctgtcgg tggtgatggc ggacaacgac
      841 gccgaagcgc ggctcaccga tgcgctgcgc gcgaccatgc aggcgaatcc atcggcgcag
      901 gtcgacttca gccggccgga actgttcagc ctgtcgccgc cgctggaaat cgaggtggag
      961 ggcaacgacc tcgagtcgat ccgcgccgcc ggcgccaagc tggccgggat gctgcgcggc
     1021 aacccgcact atgccgacgt caagtcgacc gtggagcagg gcttcccgga gatccagatc
     1081 cgcttcgacc aggaacgcgc cgccgcgctg ggcctgacca cccgccagat cgccgacgtg
     1141 gtggtcaaca aggtgcgcgg caacgtggcc acgcgctaca gcttccgcga ccgcaagatc
     1201 gacgtgctgg tgcgcgtgca ggagtccgat cgcgcatcgg tcgaagacat ccgccgcctg
     1261 atcgtgaacc ccggccgcgg cgcgccggtg gaactggcat cggtggcgga ggtggtctcc
     1321 accaccggcc ccagcgagat ccaccgcgtc gaccagcgcc gggtcgccat cgtttccgcc
     1381 aacctgcgcg acgtcgacct cggcaccgcg atcagcgaag tggagtcgat ggtgggcgcc
     1441 aatccattgg gcgccgacat cgacatgcgg atcggcggcc agggccagga actggacgaa
     1501 tcgatccgct cgctgctgtt cgcgttcgcg ctggcgatct tcctggtgta cctggtgatg
     1561 gcctcgcagt tcgaatcgct gctgcacccg ttcgtgatcc tgttcaccat cccgctggcg
     1621 ctggtgggcg cggtggccgc gctgttgctg acccgctcgc cggcctcggt ggtggtgttc
     1681 atcggcctga tcctgctggt cgggctggtg gtgaagaacg cgatcatcct gatcgacaag
     1741 gtcaaccagc tgcgcgaggc cggcgtggcc aagcgcgccg cgctggtcga gggcgcgcgt
     1801 tcgcgcctgc gcccgatcat catgaccacg atgaccgcgc tgttcggctt catgccgctg
     1861 gcgctggcgt tcggcgaagg ttccgaggtg cgttcgccga tggcgatcac ggtgatcggc
     1921 ggcctgctgg tgtcgaccct gctgaccctg gtggtgatcc cgatcgtgta cgacctgctg
     1981 gaccgcaagc cggacgaggc ctatgccgaa cgcggccgca gcgcgcgcag ggatgcgcag
     2041 gcggtggcgg aggtgctgtc gcacgaacac gatccgctcg gccatggcgg cgccgacgac
     2101 ggcgcgaagg cgtgagcggg cgatgaacat caccgagctg tcgatccgcc gcccggtcac
     2161 cacgatcatg atcttcgtgt cgctggtggt gatcggcctg atcgccgcgt tccggctgcc
     2221 gctggagtcg cagccggaag tctcgccgcc gttcttcttc gtgcagttgc cgtacccggg
     2281 ttcgacaccg gaagaggtgg agcgcaacct ggtgcggccg gtggaggaag ccctcgccac
     2341 catgcccggg atcaagtcga tgaacgcgaa cgccactgcc gacggcggcg gcgtgttcgt
     2401 gcagttcagc gactggagcc gcgacgtggc gatcgccgcc tccgaggcgc gcgagcgcat
     2461 cgacgtggtc cgcgacgaat tgccggacga cttccgacgc tatttcgtgc gccgctggag
     2521 caccggcgac caggaggcga tctcgctgcg cctgaccagc gacgacgacc tcaccgcccg
     2581 cggcgacctg atcgagcgca gcctgaagca gcgcctggag cgcctgccgg gcgtggcccg
     2641 ggtggaagtg gagggcgtgc cgaccgagga ggtgctgatc gcgatcgagc cggaccgcct
     2701 gggcgccaac ggggtcgggc tgaacgagct ggtcgcgcgc ctgcaggcgt cgaacttctc
     2761 ggtgtccgcc ggccagatca ccgatggcgg gcgccgcctg caagtccagc cgaagggcga
     2821 gctggtcgac ctgcaggcgc tgcgcgacct gccgatcgcc gccaacggca cccgcctgtc
     2881 ggacatcgcc gaggtggtga tgcagccgca gcgcatgaac tacgtgcgcc agatcgacgg
     2941 caagccggcg gtcggggtcg acatctacaa ggagcgcgcc gccaacctgg tggagctgtc
     3001 cgccgccgtg cgtcgcgagg tgcaggcgct gcagcgggac ccggcgctgc gcgggatcgc
     3061 gatcgaggtg accgacgacc agggcaagga ggtgaccagt tcgttgatcg cgcttgccga
     3121 ggcgggcggg atcggcctgc tgctgtcgat cgcggtgctg ttcttcttcc tccgccactg
     3181 gccctccacc ctgatggtga ccacggcgat cccgatctgc ttcgtgatga ccctgggctt
     3241 catgtatttc gtcggcatca ccctcaacat cctgtcgatg atgggtttgc tgctggcggt
     3301 cgggatgctg gtcgacaacg cggtggtggt ggtcgagagc atctaccagg agcgcgagaa
     3361 atacccggac cagccacgcc tggcctcggt gatcggcacc cgccacgtgg cgatcgcgct
     3421 gagcgcgggc accctgtgcc actgcatcgt gttcctgccg atgatgttcg gcgagaccaa
     3481 catgatcacc atctacctga cccagctggc ggtcaccatc tcggtgtcgc tgctggcctc
     3541 gtggctggtc gcgatcagcc tgatcccgat gctgtcggcg cgcatgcaga ccccgcccgc
     3601 ggtcaagtcg ggcctgatct cccggctgca ggagcgctac gcggcggtgt tgcgctggac
     3661 cctgggccat cgcggctgga gcgtgctcgg catcgtcctg atcagcctgc tcagcatcat
     3721 cccgatgaag atggtcaagg gcgactccgg cgacgaggag gatcccagcg agatcgcgat
     3781 cttctaccag tggcggggct cgtactcgaa ggaggaaatg ggcaaggaag tcgccagggt
     3841 cgaggcgttc ctggaagcga accgcaagcg cttccaggtc gagcaggtgt tcagccgcta
     3901 cagcgagcag ggctgggcgc agacccggat cgacatcggc gtcaacgacc ccgcggagag
     3961 caagcagatc caggaggcat tgcgcgaaag cctgccgaag tcggcgcgcg ccaacatcgg
     4021 catcggccgc cagggcgggc tgggcgcaag cgacaagggc atccagttca gcctcaccgg
     4081 cgactccacc gaaaccctgc aggaactgtc cgccgacatc atcccgatcc ttgcgcgcgg
     4141 caagcacctg cgcgacgtgc gggtggacac cggcgacagc aacagcgagc tgtcggtgcg
     4201 ggtcaaccgc gagcgcgccg ccgccttcgg cttcagcgcc gaccaggtcg cgcagttcat
     4261 cgggatggcg ctgcgcggtt cctccctgcg cgaattccag cgcggcgacg tcgagatccc
     4321 ggtcaacgtg cgcttcgccg gcgccgagca cttcggcgcg gccgacctgg agtcgttcac
     4381 ggtgcgcgcg accgacggcc gcacggtgcc gttgctggcg atggtcgatg tcagcacgcg
     4441 gcccgcgtcc accaccatcc agcggatgaa ccggcagacc atgctcaagg tcgaggccgg
     4501 gctggccagc ggcgcgacca tgccggaggc gaaggcggag atcgaagcca ccctcgggaa
     4561 cgtgcagttc ccgccgggct acggctattc gttcgagggc ggcgggttcg gcttcaacat
     4621 ggatgccaac gcgcagatgt tccgcaacat cgggatcgcg ctggtgctga tgttcatcgt
     4681 gatggcggcg gtgttcgaat cgctgctgtt cccgctggcg atcatgagtt gcgtgctgtt
     4741 ctcggtgttc ggcgtgtact ggctgttctg gctgaccggc accgagttca acatcatggc
     4801 catcatcggc atcctggtgc tgatgggggt ggtggtgaac aacggcatcg tcatggtcga
     4861 gcacatcaac aacctgcggc ggcgcgggtt gggccgcacc gatgcactgg tggaaggcag
     4921 ccgcgaacgc ctgcgtccga tcatgatgac gatgggcacg gcgatcctgg cgatggtgcc
     4981 gatcgcgttg tcggacaccc agatcgccgg cgagggccct gcctattacc cgatggcgcg
     5041 cgcgatcgcc ggtggccttg cgttctccac cgtggtcagc ctgctgttcc tgccgaccat
     5101 ctacgcgatg ctggacgacc tgcgcgcggg cagcgccaac ctgatccgga aggcgcgcag
     5161 cacgcgggcg cagcggcggg cgttggcggc ggccgccagc tagccagcgg accgcgcggg
     5221 atcacgaaag ccgcgcggca accatgcgcg gcggtggcac ccgtgccgcg cggctagacc
     5281 agcttgccga tgatggcggc gccggagatc cacacgccca ccatgctgcc caggttggtg
     5341 agcaggaaca ccagcaccgc ccggcacacc cggtttcggt accagccacc cagggtctgc
     5401 gtgtcgtcgc gcagggccag gaaatccggg taggcgggct tgcgcaaatg cacttcggcc
     5461 agcgcgctga acatgccggg cggtacgccg ggtcggaacg gcttcagcgg cgccgagacc
     5521 gccgcggcaa ggatgctcag cgggtggccg cgtgcaagca gggcgcccag cccggccagg
     5581 ccggcggtcc acgccaccca ttgcagcaac aggtccttgc ccaggtcggc gccgccctgc
     5641 gcgtagcccc aggcgatgcc accgcagatc agcaacatca gcgccagcgt gatccacggg
     5701 atgttgcgct tgcgccggac atgcgaaagc gcgccggtga gttcggccgg cgcgcgttgt
     5761 tcatcggcga ggtatgtcgc catgcccttg aggtggcccg cgccgaccac cgccagcacg
     5821 tggctcgcgc catcggcgcg ctcgcgcagt ttcgcggcca tgtactggtc gcgctcgtcg
     5881 atcagggtgg cgtacagcga gggcgtgtcg cgcgcgaatt cgccgaagct ggattcgagc
     5941 atgtcgcctt ccttcaggcg ctcgatgtcc tgctcggaca cttcttcgcg gtcgaacagg
     6001 ccgccggcca cgccggcgat cagcttggtg cggtcccacc agctcaggcc ctgcaggatg
     6061 cggcggaagg tgatgccgac atcgcgatcg atcagctgca gcggcaggtt gcgcgcggcg
     6121 gcttcgaccg cggccgcctt cagttcggcg ccgggctcga tcccgagctg ttcggccagc
     6181 cggcgctggt aggccgccaa cgccaggttg gcggcgaacg gcgcaacctt gccctcccgg
     6241 atcaccttga ccaggtccag ctgcgccagc gcgtccgggt tggtcagcgc cttgtgccgc
     6301 tgttcgtcca gttccaccgc gaccgcgtcg aagcggccgc tgtcgatcgc cgcgagcacc
     6361 gcatcgatgc tggccttcga gacgtgcgcg gtgcccagca gggtgtagtg cacgccatcg
     6421 cgttcgatct cgacgatcgg ttgcccggcg aaaccggtgt tcgccggcag cgtggattca
     6481 gtcacggtag cgcttcgtca ggtcggcgta ggcgtcgatg cggcggtcac gcaggaacgg
     6541 ccagatccgg cgcacgtgct cgctgcgctg catgtccacc tcggccagca gcagggt
//
LOCUS       DAORUK010000118         6532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_13725_length_6532_cov_19.403736, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000118 DAORUK010000000
VERSION     DAORUK010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13725_length_6532_cov_19.403736"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PK818_08115"
     CDS             <1..647
                     /locus_tag="PK818_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQY82731.1"
                     /translation="VQPSPDGLAQAFLIGREFLAGGPSCLVLGDNIFHGHGLTELLQR
                     ADARAHGATVFGYWVRDPDRYGVAEFDASGKVVGLEEKPATPKSNYAVTGLYFYDGRV
                     CDYAAALKPSRRGELEITDLNTCYLEDGSLHLEKMGRGYAWLDTGTHESLVEASTFIE
                     TIEKRQGLRVCCPEEIAYFNGWIDAARLRELAAPLAKNGYGQYLLGLLEHGRIQ"
     gene            644..1204
                     /gene="rfbC"
                     /locus_tag="PK818_08120"
     CDS             644..1204
                     /gene="rfbC"
                     /locus_tag="PK818_08120"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HQY82732.1"
                     /translation="MKIIETRLSGCVVVEPAVFGDERGFFYEGWNAQRFGQHGLPTAF
                     VQHNVSRSQKGVLRGLHYQWPDNVQGKLVSVLEGEVYDVAVDIRRGSPTFGQHVAAIL
                     SADNKRHFWIPEGFAHGFLVLSETALFTYLCTAPYDRASDNSLRWDDPALAIDWPLAE
                     VSLSPKDAAAPPLASLPAERLPLFSE"
     gene            1201..2091
                     /gene="rfbD"
                     /locus_tag="PK818_08125"
     CDS             1201..2091
                     /gene="rfbD"
                     /locus_tag="PK818_08125"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HQY82733.1"
                     /translation="MKLLLLGGTGQVGRELRRSLLPLGELLVATRDGGDADLRADFDV
                     PESLPALIARIAPDVVVNAAAYTAVDKAETDAAAAFRINADAVAAIARACAACGALLV
                     HYSTDYVFDGGATQPYREDHATTPLGAYGESKLAGEQAIRASGARHAILRTAWVYAAH
                     GKNFLLTMLRMAGEREELRVVADQVGAPTPAAWIADATAALIRQGVETSGTWHLTAGG
                     ETSWHGFAEAIIDEAQVRGLVGRKPRVVGIPTSDYPTPARRPAYSVLDTSLLQRDFGI
                     LPPDWREGLRRTLDEMAPPR"
     gene            complement(2095..3153)
                     /locus_tag="PK818_08130"
     CDS             complement(2095..3153)
                     /locus_tag="PK818_08130"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQY82734.1"
                     /translation="MTPPSIAGAWAGQRNNFNLMRLVAAWMVIYGHAHAVTGIAGNDG
                     IAWLTQLRGAGAVAVDMFFVISGFLIAASLERNSVRGYLVSRGLRILPALVVCVALTT
                     FVLGPMLTTAADYWRQPMTWNYFLVNASLLLSRFQLPGVFEGHPLDVVNGSLWTLPNE
                     AKLYLLLMVAWLLGLLAPRRYTPLWAMAMLAGYALARHYWPLPDHIQKYGECTAFFIT
                     GTLLWVNRARIPLSGWAVLGLVVAFAALRGTSWSHLPYFGLLAYGSLWLGLCVRLPMI
                     RHTDLSYGLYLYGWPAQQLAWMLPTGKTIAGNIAIATAIALACAALSWYLVERPALRL
                     KKRFVARGPAASPVAAQD"
     gene            complement(3150..3968)
                     /locus_tag="PK818_08135"
     CDS             complement(3150..3968)
                     /locus_tag="PK818_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HQY82735.1"
                     /translation="MPHLPPVPLKIALRNTPEIELWPAALLHARSNHDARALSRADRV
                     LRRKRDGAYLAADLPEGLLPLVPNLRRERGLDDALDALESTSPHARHGVEHVGELPLH
                     RLHERLDALGIDEADYAARTGLDLVAEPDWLAFAGFDRYRRPLWLRIDAARHWLRLRE
                     HALREGILLEAISGYRSHDYQLGIFARKLARGLAVDAILAVNAAPGFSEHHAGLALDI
                     GAPDEPPAEESFERTAAFAWLQEHAGEHGFAMSYPRDNPHGIVYEPWHWRYAAA"
     gene            complement(3995..4537)
                     /locus_tag="PK818_08140"
     CDS             complement(3995..4537)
                     /locus_tag="PK818_08140"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HQY82736.1"
                     /translation="MKTHGSREFAVTPVSAHAVWLMAMLLVLPAVVVVALFASQDLPD
                     PGARPGLGLALLVIVAVSLVSAVGLRRRRVALDGDHLRVVAGLFSQAVAIGAIELERA
                     RIVDLAEHSELRPAIKTLGMSLPGYQAGHFRLRAKLGKAFCLVTDTRRVLWLPMRDGK
                     QQLLLSLERPQALLDALRGR"
     gene            complement(4541..5929)
                     /locus_tag="PK818_08145"
     CDS             complement(4541..5929)
                     /locus_tag="PK818_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQY82737.1"
                     /translation="MIHLLERRHGNHRAHRIAMRLLLASMLLLMLGLGLLLPGRAAAQ
                     DATPVDGAAIATRLLDHLDAGRYADAEAMFSPEMAAAVPADKLKGVWETLPAQAGPAQ
                     GRGIATSLVRDGTTWVEQTLQYSKATLVAKLAIDAQGRIAGFLIQPAQAPVAPAAAVA
                     EDAAFVERDFSVGEGERALPGTLAMPKGDGPFAAVVLVHGSGAHDRDETVGPNKPFLD
                     IARGLAAHGIAVLRFDKRSKARPQDFANATFGVDDETTNDAVLAVDALRKAEGIDPAR
                     VFVLGHSQGGMLAPRIAAVSGHVAGLVLMAAPARSLLDIVIEQNRRLAVLDDGKTSDA
                     ERDAIAQLVEQVRVTRDPATDPATRTVMGQPAGYWRSIDAVDAVAEAKSVALPMLVLQ
                     GARDIQVVDADWGRWRGAFHANDKVAFKLYEKLNHLGIAGEGEGNLAEYSTPGHVDQA
                     LIDDVASWIKAR"
     gene            complement(5940..6110)
                     /locus_tag="PK818_08150"
     CDS             complement(5940..6110)
                     /locus_tag="PK818_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HQY82738.1"
                     /translation="MSKRWAYKVVEVKPQWLGIKPKQIEETLAPHGQQGWELVSVVNN
                     GINALLYLKKEL"
     gene            complement(6107..6292)
                     /locus_tag="PK818_08155"
     CDS             complement(6107..6292)
                     /locus_tag="PK818_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA binding domain-containing
                     protein"
                     /protein_id="HQY82739.1"
                     /translation="MSEKKAYPLRINADVLAAAQRWADDELRSLNAQIEYVLRESLRK
                     AGRLPKAAANKQDGEAK"
     gene            complement(6306..>6532)
                     /locus_tag="PK818_08160"
     CDS             complement(6306..>6532)
                     /locus_tag="PK818_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQY82740.1"
                     /translation="QAMLQRQQANAIIAARTRIVAGAVGMVQMALAELEKNGVVNLDE
                     ERKAQMVSNLLVVLCGERGTQPIVNAGTLY"
BASE COUNT          984 a         2272 c         2260 g         1016 t
ORIGIN      
        1 cggtgcagcc gagcccggac gggctggcgc aggcgttcct gatcggccgc gagttcctgg
       61 ccggcgggcc gtcctgcctg gtgctgggcg acaacatctt ccatggccac ggcctgaccg
      121 agctgctcca gcgcgccgat gcgcgcgcgc acggcgctac cgtgttcggc tactgggtgc
      181 gtgatccaga tcggtatggc gtcgccgagt tcgatgcgtc cggcaaggtg gtgggcctcg
      241 aggaaaagcc ggccacgcca aagtccaatt acgccgtcac cggcctgtat ttctacgacg
      301 ggcgtgtctg cgattacgcg gcggcattga aaccttcgcg gcgcggcgag ctggaaatca
      361 ccgacctcaa cacgtgctac ctcgaggacg gcagcctgca cctggaaaag atgggccgcg
      421 gttacgcctg gctggacacc ggcacccacg agtcgctggt cgaggcctcg accttcatcg
      481 aaaccatcga gaagcggcag gggctgcggg tgtgctgccc ggaagagatc gcctacttca
      541 acggctggat cgacgccgcc cgcctgcgtg agctggccgc gccgctggcc aagaacggct
      601 acgggcagta cctgctgggc ctgttggagc acggccgcat ccaatgaaga tcatcgaaac
      661 ccgtttgtcc ggctgcgtgg tggtcgagcc ggcggtgttc ggcgacgagc gcggcttctt
      721 ctacgaaggc tggaacgcgc agcgcttcgg ccagcacggc ctgcccaccg cgttcgtgca
      781 gcacaacgtc agccgctcgc agaagggcgt gctgcgcggg ctgcactacc agtggccgga
      841 taacgtgcag ggcaagctgg tcagcgtgct ggaaggcgag gtgtacgacg tggcggtgga
      901 tatccgtcgc ggctcgccga cgttcggcca gcatgttgcc gccatcctca gcgccgacaa
      961 caaacgccac ttctggatcc cggaagggtt cgcgcacggc ttcctggtgc tgagcgagac
     1021 ggcgctgttc acctacctgt gcaccgcgcc ctacgaccgc gccagcgaca actcgttgcg
     1081 ttgggacgat cccgcgcttg ccatcgattg gccgctggcc gaagtatcgc tgtcgcccaa
     1141 ggatgcggcc gcgccgccgc tggcaagcct gccggcggag cggcttccgc tcttcagcga
     1201 atgaagctgc tgctgctcgg cggcaccggc caggtcgggc gtgaactgcg gcgcagtctg
     1261 ttgcccttgg gcgaactgct ggttgccacg cgtgatggcg gcgatgccga cctgcgggcg
     1321 gatttcgacg tgccggaatc gctgccggca ttgatcgccc gcatcgctcc cgacgtggtg
     1381 gtgaacgcag cggcctacac tgcggtggac aaggccgaga ccgacgctgc cgccgcgttc
     1441 cgcatcaacg cggacgccgt tgcggcgatt gcacgcgcct gtgccgcctg cggcgcgttg
     1501 ctggtgcatt actcgaccga ctacgtgttc gatggcggcg ccacgcagcc gtatcgcgag
     1561 gaccacgcca ccacgccgct aggggcatat ggcgagagca agctcgcggg cgagcaggcg
     1621 atccgcgcca gcggcgcgcg ccacgcgatc ctgcgcacgg catgggtcta cgcggcgcac
     1681 ggcaagaact tcctgctgac gatgttgcgc atggcaggcg agcgcgagga attgcgcgtg
     1741 gtcgccgacc aggtcggggc gccgacgcca gccgcgtgga tcgctgacgc cacggcagcg
     1801 ttgatccggc agggtgtgga gacatccggc acctggcacc tcaccgccgg cggcgagacc
     1861 agctggcacg gtttcgccga agcgatcatc gatgaagcgc aggtgcgtgg gttggtcggc
     1921 cgcaagccgc gcgtcgtcgg catcccgacg tcggactatc cgacgccggc gcggcggcct
     1981 gcgtattcgg tactggacac gtcgctgctg cagcgcgact tcggcatcct cccgcccgat
     2041 tggcgcgaag gcttgcggcg cacgctcgat gagatggcgc cgccgcgctg aggctcagtc
     2101 ttgggccgca accggcgagg cggccggccc gcgtgccacg aaccgcttct tcagccgcag
     2161 cgcggggcgt tcgaccagat accaggacag tgccgcgcaa gccagggcaa tcgccgtcgc
     2221 gatggcgatg ttgccggcga tggtcttgcc cgtgggcagc atccacgcca gctgctgcgc
     2281 gggccagcca tacaggtaca ggccgtagga caggtcggtg tgacggatca tcggcaggcg
     2341 tacgcacagc ccgagccaca gcgacccgta ggccagcagc ccgaagtagg gcaggtgcga
     2401 ccagctcgtc ccgcgcaggg cggcgaacgc gaccaccaga cccagcacgg cccagccgga
     2461 cagcgggatg cgcgcgcggt tcacccacag cagggtgccg gtgatgaaga atgccgtgca
     2521 ctcgccgtat ttctggatgt ggtcgggcaa gggccagtaa tggcgggcga gggcatagcc
     2581 cgccagcatc gccattgccc acagcggcgt gtagcgcctg ggcgcgagca gcccgagcag
     2641 ccaggcaacc atcagcagca ggtagagctt cgcctcgttc ggcagcgtcc acagtgagcc
     2701 gttgaccacg tccagcggat ggccctcgaa cacgcccggc aactggaacc ggctcagcag
     2761 cagcgaggcg ttgaccagga aatagttcca cgtcatcggc tgccgccagt agtcggctgc
     2821 ggtggtgagc atcggcccga gcacgaacgt ggtcagtgcc acgcacacca ccagcgcggg
     2881 caggatgcgc aggccgcgcg agaccaggta gccgcgcacg ctgttgcgtt ccaggctggc
     2941 ggcgatcagg aagccgctga tgacgaagaa catgtccacc gccaccgcgc cggcgccgcg
     3001 caactgggtc agccacgcga tgccgtcgtt gccggcgatg ccggtcactg catgcgcgtg
     3061 cccgtagatc accatccacg ccgcgaccag gcgcatcagg ttgaagttgt tgcgttgccc
     3121 ggcccaggcc ccggcgatgc tgggcggcgt catgcggcgg cgtaacgcca gtgccagggt
     3181 tcgtagacga tgccgtgcgg gttgtcgcgc ggatagctca tggcgaaccc gtgttccccg
     3241 gcatgctcct gcagccaggc gaacgcggcg gtccgttcga agctttcctc ggccggcggt
     3301 tcgtccggcg cgccgatgtc cagcgccagc ccggcatggt gttcgctgaa ccccggtgcg
     3361 gcgttgacgg ccaggatggc gtcgaccgcc aggccacgcg cgagtttccg cgcgaagatc
     3421 cccagctggt agtcgtggct gcggtagccg gaaatggcct ccagcaggat gccctcgcgc
     3481 agcgcatgtt cgcgcaggcg cagccagtgg cgcgcggcgt cgatgcgcag ccacagcggg
     3541 cgtcggtagc ggtcgaagcc ggcgaacgcc agccaatcgg gttccgcgac caggtccagg
     3601 ccggtgcgcg cggcgtagtc cgcctcgtcg atgccgagcg cgtccagccg ttcgtgcagg
     3661 cggtgcagcg gcagttcgcc gacatgctcc accccgtggc gcgcatgcgg tgatgtcgat
     3721 tccagtgcgt ccagggcatc gtccaggccg cgttcgcggc gcaggttcgg caccagtggc
     3781 aacaggcctt ccggcaggtc cgcggccagg taggcgccgt cgcgcttgcg gcgcagcacg
     3841 cgatcggcgc gggacagcgc gcgcgcatcg tggttgctgc gcgcgtgcag cagcgcggcc
     3901 ggccagagtt cgatctccgg ggtgttgcgc agcgcgatct tcagcggaac cggtggcagg
     3961 tggggcatcc gcgcaggata cccgggtgcg ccggtcagcg tccgcgcagg gcgtccagca
     4021 acgcctgcgg gcgctccagg ctgagcagga gctgctgctt gccatcgcgc attggcagcc
     4081 acagcacccg gcgggtgtcg gtcaccaggc agaacgcctt gccgagcttt gcgcgcagcc
     4141 ggaagtgccc ggcctggtag ccgggcaggg acatgccgag ggtcttgatc gccggccgca
     4201 gctcgctgtg ttcggcgagg tcgacgatgc gtgcgcgctc gagctcgatc gcgccgatcg
     4261 ctaccgcctg cgagaacagg ccggccacca cccgcaggtg gtcgccatcc agcgcgaccc
     4321 gccggcgcct caggccgaca gcactgacca gcgagacggc gacgatcacc agcagcgcaa
     4381 ggccgagacc cggccgcgcg cccggatccg gcaggtcctg gctggcgaac agcgccacca
     4441 ccacgaccgc ggggagcacc agcagcatgg ccatcagcca caccgcatgt gccgataccg
     4501 gggtcaccgc gaactcgcgc gaaccgtggg tcttcatcgc tcagcgcgcc ttgatccagc
     4561 tggcgacgtc gtcgatcagt gcctgatcga catggcccgg cgtgctgtat tcggccaggt
     4621 tgccttcgcc ttcgccggcg atgccgaggt ggttgagctt ttcgtacaac ttgaacgcga
     4681 ccttgtcgtt ggcatggaat gcgccgcgcc agcggcccca gtcggcgtcc accacctgga
     4741 tgtcgcgcgc gccctgcagc accagcatcg gcaacgccac cgacttcgcc tcggcgacgg
     4801 catccaccgc gtcgatgctg cgccagtagc cggccggctg gcccatcacg gtcctggtgg
     4861 cggggtcggt ggccgggtcg cgggtcacgc gcacctgttc gaccagttgc gcgatggcgt
     4921 cgcgctcggc gtcgctggtc ttgccgtcgt caagcaccgc aagccggcgg ttctgctcga
     4981 tcacgatgtc cagcagcgag cgtgccggcg ccgccatcag caccagcccg gcgacgtgcc
     5041 cggacaccgc ggcaatccgc ggcgccagca tcccgccctg gctgtggcca agcacgaaca
     5101 cgcgcgcggg atcgatgcct tcggccttgc gcagcgcatc caccgccagc acggcgtcat
     5161 tggtggtttc gtcgtcgacg ccgaacgtgg cgttggcgaa gtcctgcggt cgcgccttgc
     5221 tgcgcttgtc aaagcgcagg accgcgatgc cgtgcgcggc cagcccgcgg gcgatgtcga
     5281 ggaacggctt gttggggccc acggtttcgt cgcggtcgtg cgcgccggag ccgtgcacca
     5341 gcaccaccgc ggcgaacggg ccgtcaccct tgggcatcgc caacgtgccg ggcagggcgc
     5401 gttcgccctc gccgacgctg aagtcgcgtt cgacgaacgc agcatcctcg gccactgccg
     5461 cggccggcgc gaccggtgcc tgcgcgggct ggatcaggaa gcccgcgatc ctgccctgcg
     5521 cgtcgatcgc cagcttcgcc accagcgtcg ccttgctgta ctgcagggtc tgttccaccc
     5581 aggtggtgcc atcccggacc aggctggtgg cgatgccgcg cccctgcgca ggacccgcct
     5641 gcgcgggcag cgtttcccac acccccttca gcttgtccgc cggcactgcc gcggccatct
     5701 ccgggctgaa catcgcctcg gcatccgcat agcggcctgc atcgaggtgg tcgagcaggc
     5761 gggtcgcgat cgcggcgcca tcgacgggcg tggcgtcctg cgccgcggcg cgccccggca
     5821 gcaacaggcc cagcccgagc atcagcagca gcatcgacgc cagcagcagg cgcatcgcga
     5881 tgcggtgcgc acggtggttg ccgtggcggc gttcaagcag gtggatcatg tgcagcacgt
     5941 cagagctcct tcttcagata cagcagcgcg ttgatcccgt tgttcaccac cgagaccagc
     6001 tcccagccct gctggccgtg cggcgccagc gtctcttcga tctgcttcgg cttgatgccc
     6061 agccattgcg gcttgacctc gaccaccttg tacgcccagc gcttgctcat tttgcttccc
     6121 cgtcctgctt gttggcggcc gccttcggca gccggccggc cttccgcaac gattcgcgca
     6181 gcacgtactc gatctgcgcg ttgaggctgc ggagctcatc gtcggcccag cgctgcgccg
     6241 ccgccaggac gtcggcattg atgcgcagcg gataggcctt cttctcgctc acgaccggtg
     6301 ccggctcagt acagggtgcc ggcgttgacg atcggctggg tgccgcgctc gccgcacagc
     6361 accaccagca ggttgctgac catctgcgcc ttgcgctctt cgtccaggtt gaccacgccg
     6421 ttcttctcca gctccgccag cgccatctgc accatgccga ccgcgccggc gacgatccgc
     6481 gtgcgcgcgg cgatgatcgc gttggcctgc tggcgctgca gcatcgcctg gg
//
LOCUS       DAORUK010000119         6498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_13832_length_6498_cov_20.499612, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000119 DAORUK010000000
VERSION     DAORUK010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13832_length_6498_cov_20.499612"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..1289
                     /gene="hppD"
                     /locus_tag="PK818_08165"
     CDS             189..1289
                     /gene="hppD"
                     /locus_tag="PK818_08165"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017112910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HQY82741.1"
                     /translation="MSAQPASNSMNPGMQVTTFENPMGIDGFEFVEFAVPPGHAEAMH
                     RYLCSMGFSATRRHRARAITLYRQGGVNFLLNEAPDSFASDFAAAHGPSACGFAIRFH
                     APVASVLETVIGNGGEVIDHKPQTRAIDAPVVKGIGDCMLYLVDAEAGDPYADYVAIE
                     GAGQDPRGFGLTFIDHLTHNLYFGNMQKWSDYYEKLFNFREIRYFDIKGAKTGLVSKA
                     MTAPDGIVRIPLNESSDPKSQINEYLDAYHGEGIQHIALFTDDIYASVEAMHAAGVQF
                     LDTPDTYFEVIDLRVPGHGEDVARLARNKILIDADPETHQRKLLQIFTQNAFGPIFFE
                     IIQRKGNEGFGEGNFQALFESIERDQMRRGVL"
     gene            1303..2598
                     /gene="hmgA"
                     /locus_tag="PK818_08170"
     CDS             1303..2598
                     /gene="hmgA"
                     /locus_tag="PK818_08170"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HQY82742.1"
                     /translation="MAIASTGYQSGFGNEFASEALAGTLPDGRNSPQRVAHGLYAEQL
                     SGTAFTAPRHSNRRSWLYRIRPAAIHGPFALLDHAAFHNDFGSGPVTPDQLRWNPLPM
                     PEAAADFVDGLVTMAGNGSPASQSGIGIHLYAANRDMHGRFFYDADGELLVVPQQGRL
                     HIETELGVLDVAPQEIAVIPRGVRFRVALPDGSARGYVCENFGAFLRIPDLGPIGSNG
                     LANPRDFLTPNAAFEDVEGDFELVAKFQGHLWRAAIGHSPLDVVGWHGNHVPCKYDLR
                     LFNTIGSISYDHPDPSIFLVLTSPTDTPGVGNLDFAIFPPRILAAQDTFRPPWFHRNI
                     ASEFMGLVHGEYDAKAEGFAPGGCSLHNCMTGHGPDASTFDKASNADTSKPDYIVDTM
                     AFMFETRNVIRPTRQALDAGHRQHDYQACWTGLRKHFRG"
     gene            2651..3880
                     /gene="nhaA"
                     /locus_tag="PK818_08175"
     CDS             2651..3880
                     /gene="nhaA"
                     /locus_tag="PK818_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005393226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HQY82743.1"
                     /translation="MSDEPTILNRARHALGRFLQLEASAGILLIGAALVALAWANSPL
                     AATYDGIRGVPVAVQFGALKIAKPLLLWINDGLMAIFFLLVALELKREALSGQLSTPS
                     QLSLPVLCALAGVAVPAAIYAWINRGDPQAMQGWAVPAATDIAFALGILSLLGSRVPV
                     AMKLLLSTIAVIDDLAAIIIIALFYTSELSVLALAWAGAAIAAMVVLNRRGVQALTPY
                     LLLGLVAWVAVLKSGVHATLAGVATGLLIPHLDRKDAIDDETQHSPLETLEHALHPWV
                     AYLILPVFAFANAGLALGGFALADVLAPVPLGVALGLVLGKPVGVLAAAGLARASGIA
                     RFPEGMDWKAMVGLGLLCGIGFTMSLFIASLAFADDGGLFPSAVLGVLCASLVSALAG
                     YAWLRVTLPPGPSETAG"
     gene            3936..5342
                     /gene="nhaD"
                     /locus_tag="PK818_08180"
     CDS             3936..5342
                     /gene="nhaD"
                     /locus_tag="PK818_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HQY82744.1"
                     /translation="MRLAAGALLAAMPLAASAASPVQAGFASHWVGYVALLWFVVAYL
                     FVIAEEFTGLRKSQPVMLAAGAIWALLAFASAQAGQSAELHAAVRDYLLEFAELLLFL
                     LAAMTYVNAMSERGLFEALRVWLLRRGLGYRALFWATGALAFFLSPLIDNLTTALVMC
                     AVVLAVGRDDPRFVALACINIVVAANAGGTFSPFGDITTLMVWQAGKVPFAGFFALFL
                     PALVNWGVPALCMHAAVPRGTPALADEQVRLRRGAWVIVILFGLTVALAVGFHQFLHL
                     PPFMGMMTGLALLKLYGWHLTRVANAQQASEFAREGAPGDIDAFDSYEQVARAEWDTL
                     LFFFGVIMCVGALGYCGYLALLSQQFYGGLGPTTANVLVGLMSAVLDNIPMMVAVLQM
                     APEMDGGQWLLVTLTAGVGGSLLSIGSAAGVALMGQAHGQYTFFSHLKWSWAIALGYA
                     ASVAVHLLVNARLFTGLA"
     gene            5350..6225
                     /locus_tag="PK818_08185"
     CDS             5350..6225
                     /locus_tag="PK818_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar epimerase"
                     /protein_id="HQY82745.1"
                     /translation="MKTALVFGASGQLGAPLLERLNDAGWRVFAVSRQSHSDAPGRHW
                     LGGDLAGVRGLPDAVDAIFSCGPLDLFARWHAQATIRAPRIVAFGSTSAATKHGSADD
                     DERDLARRLLGAEAALFAQAELRGAHATMLRPTLVYGAGRDATLTRIAALAGRWGHFL
                     LPRRSDGLRQPVHVHDLAAAAFAACTAAATHGRSYDLPGGETLPYREMVQRVLACLQP
                     RPVLHELPMPLFRLALEAARARGIATDISAAAVERMRHDMLFDPQPAQRDFGFAPRAF
                     KPEARMFENSIPSRE"
     gene            complement(6322..>6498)
                     /locus_tag="PK818_08190"
     CDS             complement(6322..>6498)
                     /locus_tag="PK818_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HQY82746.1"
                     /translation="GLARLALGVFVLAWIAQFIGHGKRYEGKRPSFFTDLKYLLVGPA
                     WVLAKLYRKLGWSW"
BASE COUNT          925 a         2249 c         2278 g         1046 t
ORIGIN      
        1 gggcgatcgc gctgctgatc cggttggaca gcacgctcag ccggtacggc aggaagtggt
       61 cgaggtccag ggtcgggtgc gcctgcgcgg cgggcctggg tttgccgggt ggggacatct
      121 gctgcaccgc cgcttgagat ggttgcaagt gaaactataa gatggacggc cccagcgagg
      181 aagcctccat gagcgcccag ccagcttcga acagtatgaa ccccggcatg caggtcacca
      241 ccttcgagaa cccgatgggg atcgacggct tcgagttcgt cgaattcgcg gtcccgccgg
      301 gccacgccga ggccatgcac cgctacctgt gcagcatggg cttcagcgcg acccgccgcc
      361 acagggcccg cgcgatcacc ctgtaccggc agggcggggt gaacttcctg ctcaacgagg
      421 cgccggactc gttcgcgtcg gatttcgccg ccgcgcacgg cccgtccgcc tgcggcttcg
      481 cgatccgctt ccacgcaccg gtcgcgtccg tgctggaaac ggtgatcggc aacggcggcg
      541 aagtgatcga ccacaagccg cagacgcgcg cgatcgacgc gccggtggtg aagggcatcg
      601 gcgactgcat gctgtacctg gtggatgccg aggccggcga cccgtatgcc gactacgtcg
      661 ccatcgaggg cgccgggcag gacccgaggg gcttcggcct gaccttcatc gaccacctga
      721 cccacaacct ctacttcggc aacatgcaga agtggtcgga ctactacgag aagctgttca
      781 acttccgcga gatccgctac ttcgacatca agggcgccaa gaccgggctg gtgtcgaagg
      841 cgatgaccgc gccggacggc atcgtgcgca tcccgctcaa cgaatccagc gacccgaagt
      901 cgcagatcaa cgaatacctc gacgcctacc acggcgaggg catccagcac atcgcgctgt
      961 tcaccgacga catctacgcc tcggtggagg cgatgcacgc cgccggcgtg cagttcctgg
     1021 acaccccgga cacctacttc gaggtgatcg acctgcgggt accgggccac ggcgaggacg
     1081 tggcgcggct ggccaggaac aagatcctga tcgatgccga tccggagacc caccagcgca
     1141 agctgctgca gatcttcacc cagaacgcgt tcggcccgat cttcttcgag atcatccagc
     1201 gcaagggcaa cgagggcttc ggcgaaggca acttccaggc gctgttcgag agcatcgagc
     1261 gcgaccagat gcgccgcggc gtgctgtaag ggacacgaca ccatggccat cgccagcacc
     1321 ggataccaga gcggcttcgg caacgaattc gccagcgaag cgcttgccgg caccctgccg
     1381 gacgggcgca actcgccgca gcgcgtggcc cacggcctgt acgcggagca gctcagcggc
     1441 accgcgttca ccgcgccgcg gcacagcaac cgccgcagct ggctgtaccg gatccgcccg
     1501 gcggcgatcc acgggccgtt cgccctgctc gaccacgcgg ccttccacaa cgatttcggc
     1561 agcggcccgg tgaccccgga ccagctgcgc tggaacccgc tgccgatgcc ggaggccgcg
     1621 gccgacttcg tcgatggcct ggtcacgatg gccggcaacg gttcgccggc ttcgcagtcc
     1681 ggcatcggca tccacctgta cgcggccaac cgcgacatgc acggccgctt cttctacgac
     1741 gccgacggcg aactgctggt cgtgccgcag cagggccgcc tgcacatcga aaccgaactc
     1801 ggcgtgctgg acgtggcacc gcaggaaatc gcggtgatcc cgcgcggagt ccgcttccgg
     1861 gtggcgctgc cggatggcag cgcgcgcggc tacgtgtgcg agaacttcgg cgcgttcctg
     1921 cggatcccgg accttggccc gatcggcagc aacgggctgg ccaacccgcg cgatttcctg
     1981 accccgaatg cggcgttcga ggacgtcgag ggcgacttcg aactcgtcgc caagttccag
     2041 ggccacctgt ggcgcgcggc catcggccat tcgccgctgg acgtggtcgg ctggcacggc
     2101 aaccacgtgc cctgcaaata cgacctgcgg ctgttcaaca ccatcggttc gatcagctac
     2161 gaccacccgg acccttcgat cttcctggtg ctgacctcgc ccaccgacac cccgggggtc
     2221 ggcaacctgg acttcgcgat cttcccgccg cggatcctgg ccgcgcagga cacgttccgc
     2281 ccgccgtggt tccaccgcaa catcgccagc gagttcatgg gcctggtgca tggcgaatac
     2341 gacgccaagg cggagggttt cgcgcccggc ggctgctcgc tgcacaactg catgaccggc
     2401 cacggcccgg acgcttcgac cttcgacaag gccagcaacg ccgacacctc gaagccggat
     2461 tacatcgtcg acaccatggc cttcatgttc gagacccgca acgtgatccg gccgacccgg
     2521 caggcactcg acgccggcca ccggcaacac gattaccagg cctgctggac cggattgcgc
     2581 aagcacttcc gggggtgaac gcggccggcc ggcggcgaca cgccggccac gcttcgtata
     2641 ctgcggtgca atgagcgatg aaccgaccat cctgaatcgc gcccggcatg cactgggacg
     2701 cttcctgcag ctggaggcgt cggccggcat cctgctgatc ggcgcggcgc tggtggcgct
     2761 ggcctgggcc aactcgccgc tggccgcgac ctacgacggc atccgcgggg tgccggtggc
     2821 agtccagttc ggcgcgctca agatcgccaa gccgctgctg ctgtggatca acgacggcct
     2881 gatggcgatc ttcttcctgc tggtggcgct ggagctcaag cgcgaggcgc tgtccggcca
     2941 gctgtccacc ccctcgcaac tgtcgctgcc ggtgctgtgc gcgctggccg gggtggcggt
     3001 gccggcggcc atctacgcct ggatcaaccg cggcgacccc caggcgatgc agggctgggc
     3061 ggtgccggcg gcgaccgaca tcgcgtttgc gctggggatc ctgtcgctgc tgggctcgcg
     3121 ggtgccggtg gcgatgaagc tgctgttgtc caccatcgcg gtgatcgacg accttgccgc
     3181 gatcatcatc atcgcgctgt tctacaccag cgagctgtcg gtgctggcgc tggcgtgggc
     3241 gggcgcggcg atcgcggcga tggtggtgct gaaccggcgc ggggtgcagg cgctgacgcc
     3301 gtacctgctg ctggggctgg tggcgtgggt ggcggtgctc aagtccggtg tccacgcaac
     3361 cctggccggg gtcgccaccg gcctgctgat cccgcacctg gaccgcaagg acgcgatcga
     3421 cgacgagacc cagcattcgc cgctggaaac gctggagcac gcgctgcacc cgtgggtggc
     3481 gtacctgatc ctgccggtgt tcgcgttcgc caatgccggg ctggcgctcg gcggcttcgc
     3541 gctggccgac gtgctcgcgc cggtaccgtt gggggtggcg ctgggactgg tgctgggcaa
     3601 gccggtcggg gtgctggccg cggccgggct ggcgcgtgcc agcgggattg cacggttccc
     3661 cgaaggcatg gactggaagg cgatggtcgg gctggggctg ctgtgcggga tcggcttcac
     3721 catgagcctg ttcatcgcct cgctggcgtt cgccgacgac ggcggcctgt tcccgagcgc
     3781 ggtgcttggc gtgctgtgcg cctcgctggt gtcggcgctg gccggctacg cctggttgcg
     3841 ggtgaccttg ccgcccggcc catcggaaac cgcgggttag actcccgcaa ttgcctccac
     3901 ccggttccgt gatgccctcg atcccactgc cggccatgcg cctggccgcc ggcgcgctgt
     3961 tggcggcgat gccgctggcg gcctcggcgg cgtccccggt gcaggccggg ttcgcctcgc
     4021 actgggtggg ctatgtcgcc ctgctttggt tcgtggtcgc ctacctgttc gtgatcgccg
     4081 aggaattcac cgggctgcgc aagtcgcagc cggtgatgct ggccgcgggc gcgatctggg
     4141 ccctgctggc gtttgcgtcc gcgcaggccg ggcagtccgc ggaactgcat gcggcggtgc
     4201 gcgactacct gctggaattc gccgagttgc tgctgttcct gctggcggcg atgacctacg
     4261 tcaacgcgat gagcgagcgc ggcctgttcg aggccctgcg cgtgtggttg ctgcgcaggg
     4321 ggctggggta tcgcgcgctg ttctgggcca ccggcgcgct ggcgttcttc ctgtcgccgc
     4381 tgatcgacaa cctgaccacc gcgctggtga tgtgcgcggt ggtgctggcg gtgggccgcg
     4441 acgatccgcg cttcgtcgcg ctggcgtgca tcaacatcgt ggtggcggcc aacgccggcg
     4501 gcaccttcag cccgttcggc gacatcacca ccctgatggt ctggcaggcg ggcaaggtgc
     4561 cgttcgccgg cttcttcgcg ctgttcctgc cggcgctggt gaactggggg gtgccggcac
     4621 tgtgcatgca cgcggcggtc ccgcgcggca ccccggcgct ggccgacgag caggtgcgcc
     4681 tgcgccgcgg ggcgtgggtg atcgtcatcc tgttcgggct gacggtggcg ctggcggtgg
     4741 ggttccacca gttcctgcac ctgccgccat tcatggggat gatgaccggg ctggcgctgc
     4801 tcaagctgta cggctggcac ctgacccgcg tcgccaacgc ccagcaggcc agcgagttcg
     4861 cgcgcgaggg cgcgcccggc gacatcgacg cattcgacag ctacgagcag gtcgcgcgcg
     4921 ccgagtggga caccctgctg ttcttcttcg gggtgatcat gtgcgtgggc gcgctgggtt
     4981 actgcggcta cctggccctg ctgtcgcagc agttctacgg cggattgggg ccgaccaccg
     5041 ccaacgtgct ggtggggctg atgtcggcgg tgctggacaa catcccgatg atggtggcgg
     5101 tgctgcagat ggcgccggag atggatggcg gccagtggct gctggtgacc ctgaccgccg
     5161 gcgtcggcgg cagcctgctg tcgatcggct ccgccgccgg ggtggcgctg atgggccagg
     5221 cgcacggcca gtacacgttc ttcagccacc tcaagtggag ctgggcgatc gcgctgggct
     5281 atgccgccag cgtcgcggtg cacttgctgg tgaacgcgcg cctgttcacc gggctggcgt
     5341 gagcgcggca tgaagacagc cctggtcttc ggtgccagcg gccagcttgg cgcacccttg
     5401 ctggagcgcc tgaacgacgc cggctggcgg gtcttcgcgg tttcccggca gtcgcattcg
     5461 gatgcgcccg gccggcactg gctgggcggc gacctggccg gcgtgcgcgg cctgccggac
     5521 gcggtggacg cgatcttcag ctgcggcccg ctggacctgt tcgcgcgctg gcacgcgcag
     5581 gccacgatcc gggcgccgcg catcgtcgcg ttcggctcca ccagcgcggc caccaagcat
     5641 ggctccgcgg acgacgacga acgcgacctc gcgcgccggc tgctgggcgc ggaggcggcg
     5701 ctgttcgcgc aggcggaact gcgcggcgcc cacgcgacca tgctgcgccc gaccctggtg
     5761 tacggcgccg gccgcgatgc caccctcacc cgcatcgccg cgctggccgg ccgctggggc
     5821 cacttcctgc tgccccggcg cagcgatggc ctgcgccagc cggtgcacgt gcacgacctc
     5881 gccgccgccg cgttcgcggc ctgcaccgcg gcggcgaccc acggccgcag ctacgacctg
     5941 ccgggtggcg agaccctgcc gtaccgggag atggtgcagc gcgtgctggc ctgcctgcag
     6001 ccgcggccgg tcctgcacga attgccgatg ccgctgttcc ggctggcact ggaagcggca
     6061 cgcgcgcgtg gcatcgcaac cgacatcagc gccgccgcgg tcgaacgcat gcgccacgac
     6121 atgctgttcg acccgcagcc ggcgcagcgg gatttcggct tcgcgccgcg cgcgttcaag
     6181 ccggaagcgc ggatgttcga gaactcgatt ccaagtcgcg agtgactacc cacgatggcg
     6241 agaccgtcgg gtgttggcgt cgccgcatct tgagccgcgg tgcgaacacg cgacggtagg
     6301 cgccatcgca gcctcaggcc ctcaccagga ccagccgagc ttcctgtaca gcttggccag
     6361 cacccaggcc ggaccgacca gcaggtactt gaggtcggtg aagaagctcg gccgcttgcc
     6421 ctcgtagcgc ttgccgtggc cgatgaactg cgcgatccag gcgagcacga acacgcccag
     6481 cgccagcctg gccaggcc
//
LOCUS       DAORUK010000120         6480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_13903_length_6480_cov_20.015720, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000120 DAORUK010000000
VERSION     DAORUK010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13903_length_6480_cov_20.015720"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PK818_08195"
     CDS             <1..485
                     /locus_tag="PK818_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQY82747.1"
                     /translation="PKSEPFSKIALDAAQAAAHHQRHDGSRLAESLNRSEFVDRALRQ
                     AVTRESMRLETGLTVLATVGSTAPFVGLLGTVWGIYRALIKIGASGDASISAVAGPVG
                     EALIMTAIGLFVAIPAVLAYNFFVRLNRVTNNQFDTFAHDLHDFFATGSRVGEVAGKR
                     "
     gene            516..944
                     /locus_tag="PK818_08200"
     CDS             516..944
                     /locus_tag="PK818_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQY82748.1"
                     /translation="MSFSSGNESGGPMAEINVTPLVDVMLVLLIIFMITTPLMNHRVK
                     VQLPEAVVDKKEEKKQAVPPVTIAVTESGALFWNDEPATRQLLESKLSVEAQKTPQPP
                     VNIRGDKTTPYRLINDVVKIAQAQGIAKVGFVTTPPKKNL"
     gene            955..1368
                     /locus_tag="PK818_08205"
     CDS             955..1368
                     /locus_tag="PK818_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQY82749.1"
                     /translation="MAFASNSGGGAMADINVTPLVDVMLVLLIIFMVTMPIQSIPVDV
                     DLPQKTEKPPENPKEPPDPIELRIDAAGQVFWNNTPTPITALQQMMRNEVDRDPTNQP
                     TLEIDTNEAAEYGVLAKVLTAAKNAEMVKIGFVQN"
     gene            complement(1439..2194)
                     /locus_tag="PK818_08210"
     CDS             complement(1439..2194)
                     /locus_tag="PK818_08210"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007968197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HQY82750.1"
                     /translation="MSTALSVNLNKIAVLRNSRGGSEPDVARAGIACLDAGAHGLTVH
                     PRPDARHIREDDVHVLAAVCHARGVELNIEGNPFAPARAGYPGLLALCGQARPAQVTL
                     VPDGDGQLTSDHGFDFRRDGAALRPLVAAFRALGCRVSVFVDAGDPEVARAADCGADR
                     VELYTGPFAEAFAAGDAAETLAQAAATARRAQAAGLAVNAGHDLSQANLGAFLAAVAD
                     VREVSIGHALVGEALYAGLDATVRSYRRIVDAA"
     gene            complement(2191..2448)
                     /locus_tag="PK818_08215"
     CDS             complement(2191..2448)
                     /locus_tag="PK818_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82751.1"
                     /translation="MHWLYLLLALAALVLAIKTTSTSLMVVSLLASLVLLLLWLAGLY
                     SARMAGRSRDPSAMIDPTELRRLREQAEARKAANQSESQSP"
     gene            complement(2510..3949)
                     /gene="cls"
                     /locus_tag="PK818_08220"
     CDS             complement(2510..3949)
                     /gene="cls"
                     /locus_tag="PK818_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HQY82752.1"
                     /translation="MPTLLSSWEALWAIPHIGLYVGLGWLAYLLGLGVWIVLQKREPA
                     ATISWLVSLAALPYIGFFVYHVFGPQKIRRHRLRRSRARVGMPGPPGEGEHEAVELAR
                     LGQATTGLPPTTAQRVDLLIDGAATYDALLDAVRGARDHVHLEYYIYHPDRSGTALRD
                     ALVERARAGVKVRLLLDAVGSAGTADAFFADLRAAGGELAWFHPMRFGHVWKRPWLNM
                     RTHRKIVVVDGRVGFAGGINITDEENERLRADAYRDLHLRIEGDSVRALQLVFVEDWV
                     YATGSRDFIGEVARAMPPPAHGPVHAQVLTSGPDSDWEAIHRLHVGAIHAAKRRVWLV
                     TPYFVPGEAAMMALTSAGFGGLDVRLLVPKTSDSTLVTYAARSYFDSLLEAGVKVYEY
                     GPRMLHTKALLVDDELALVGSANFDHRSFRLNFEVCLLIRDAGVAGDLAKLIEGEFAQ
                     APRVRADRERPLWRARLPEAFARLLSPLL"
     gene            complement(4081..5898)
                     /locus_tag="PK818_08225"
     CDS             complement(4081..5898)
                     /locus_tag="PK818_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQY82753.1"
                     /translation="MLSTYPLLSSQIAFGLILAVGLYLFISERLRVDVTAMLILLALV
                     LTGVLDSKQALSGFASEPAIIVAAVFVISGALAATGISERLGAWIGRAAGVHEWRTIA
                     VVMPAVALLAAFTHHVMVTAMMLPLLLRHARDRQLPASRLLMPMSLAASLGTTLTLFS
                     APAFLLASDMLQRAGTDGLGIFSITPIGIALVLVGTSYMLGARWLLPKRSGTHGDDDY
                     LRLDRYRTELLVVKDGRWTTRPLSELHKALGARFRLLGWLRDGVRRDDLTATSPLLAG
                     DVLLVEASADELLSLHDDPGLDLNAIVRFNDTVSGEGAPQLVQAVVAPGSEFIGRSIR
                     ELDFARQFHAVIAGLWRREGDMADRLSDARLREGDLLVLYGRPSRFSDLAAHHGFLML
                     VPFAGEAKRRVHAPLALLILGVTILAAATEWLSAPLAFLMGAVAMVSTRCVDIQQAYR
                     GIDVRIFVMIAGVIPLGIAMEQTGTADLLARGLSHMVAGWPALPILLVMFTAAALLTQ
                     VMSDAATTVLLGPVAISLARTLDLPPTPFVVCTALGAVVAFLTPIGHHGNLLILGPGQ
                     YRFGDFLRIGLPLTVVIALVSAWMARWLWLGGPLLPLSG"
     gene            complement(6161..>6480)
                     /locus_tag="PK818_08230"
     CDS             complement(6161..>6480)
                     /locus_tag="PK818_08230"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HQY82754.1"
                     /translation="PRGKDFNMRWVASMVADVHRILTRGGIFSYPLDSKCAPKGGKLR
                     LMYEANPMALLVEQAGGAASTGRQRMLEVQPTGLHQRVPVFLGSKAEVEAAVRYHHAA
                     DAA"
BASE COUNT         1068 a         2290 c         2252 g          870 t
ORIGIN      
        1 agccgaagtc cgagccgttc tccaagatcg ccctcgacgc cgcccaggcc gccgcccacc
       61 accagcgcca cgatggttcg cgcctggccg agtcgctgaa ccgctccgag ttcgtggacc
      121 gcgccctgcg ccaggccgtg acccgcgaga gcatgcgcct cgagaccggc ctgaccgtgc
      181 tggccaccgt cggttcgacc gcgccgttcg tcggcctgct cggtaccgtt tggggcatct
      241 accgcgccct gatcaagatc ggcgcctccg gcgacgcctc gatctccgcg gtggcgggtc
      301 cggtcggtga agccctgatc atgaccgcga tcggtctgtt cgtggcgatc ccggcggtgc
      361 tggcgtacaa cttcttcgtg cgcctgaacc gcgtgaccaa caaccagttc gacaccttcg
      421 cgcacgacct gcacgacttc ttcgccaccg gctcgcgcgt cggcgaagtg gccggcaagc
      481 gctgatcgac ctgactgctt cgacggagcc ccaccatgtc cttcagttcc ggtaacgaaa
      541 gcggcggccc gatggccgag atcaacgtca cgcccctggt ggacgtgatg ttggtgctgc
      601 tgatcatctt catgatcacc accccgctga tgaaccatcg ggtcaaggtg cagctgccgg
      661 aagcggtggt cgacaagaag gaagagaaga agcaggcggt cccgccggtc accatcgcgg
      721 tgaccgagtc cggggcgctg ttctggaacg atgaaccggc gacccgccag ctgctggaaa
      781 gcaagctgtc ggtcgaggcg cagaagaccc cgcaaccgcc ggtcaacatc cgcggcgaca
      841 agaccacgcc gtatcgtttg atcaacgacg tggtcaagat cgcccaggcc cagggcatcg
      901 ccaaggtcgg cttcgtcacc acgccgccca agaagaacct gtaaggagac cgccatggca
      961 tttgcaagca attcgggcgg tggggcgatg gccgacatca acgtcacgcc cctggtggac
     1021 gtgatgctgg tgctgctgat catcttcatg gtgaccatgc cgatccagtc gatcccggtc
     1081 gacgtcgacc tgccgcagaa gaccgagaag ccaccggaga acccgaagga accaccggat
     1141 ccgatcgagc tgcgcatcga cgccgccggc caggtgttct ggaacaacac gccgaccccg
     1201 atcactgccc tgcagcagat gatgcgcaac gaggtcgacc gcgatccgac caaccagccg
     1261 accctcgaga tcgacaccaa cgaagccgcc gaatacggcg tgctggcgaa ggtcctgacc
     1321 gcggccaaga acgccgaaat ggtcaagatc ggtttcgtcc agaactgaac cagacaactc
     1381 aggctccgct caggagcccg tcgtggatga cggcgccgcc cgcgagggcg gcgttttttt
     1441 atgcggcgtc gacgatccgg cgatagctgc ggacggtggc gtccagcccc gcgtacagcg
     1501 cctcgccgac cagcgcgtgg ccgatcgaca cttcgcgcac gtccgcaacc gccgccagga
     1561 acgcaccgag gttggcctgg ctgaggtcgt ggccggcatt caccgccagt cccgcggcct
     1621 gcgcgcggcg cgcggtggcc gcggcctgtg ccagggtttc agcggcatcg ccggcggcga
     1681 aggcctccgc aaacgggccg gtatacagct cgacgcggtc ggcgccgcag tcggcggcac
     1741 gcgcgacctc ggggtcgccg gcatcgacga acacgctgac ccggcaccca agcgccctga
     1801 acgcggcgac cagcggccgc aacgcggcgc catcgcgccg gaaatcgaaa ccgtggtccg
     1861 aggtcagctg gccgtcgcca tcagggacca gcgtgacctg cgccggccgg gcttgcccgc
     1921 acagggccag cagccccgga tagccggcgc gcgccggcgc gaacggattg ccctcgatgt
     1981 tgagctcgac cccgcgcgca tggcacaccg ccgccagtac gtggacgtcg tcttcgcgaa
     2041 tgtggcgtgc gtcggggcgc ggatgcacgg tcaggccatg cgcgccggca tccaggcagg
     2101 cgatccccgc gcgcgccacg tcgggttcgc tgccgccgcg tgaattgcgc aggaccgcga
     2161 tcttgttgag gttgacgctg agggcggtgc tcaaggcgac tgcgattcgg actggttcgc
     2221 ggccttgcgc gcctccgcct gttcgcgcag gcggcgcagt tcggtggggt cgatcatcgc
     2281 cgagggatcg cggctgcggc cggccatgcg cgccgagtac agcccggcca gccacagcag
     2341 cagcaacacc agcgacgcca gcaggctcac caccatcagg ctggtggagg tggtcttgat
     2401 ggcgagcacc agcgcggcga gcgcgagcag gaggtacagc caatgcatgc aggcggctcc
     2461 gggcggaatt ggcggcagtg tacgcccgcg gccggcgccg aggaatccgt cagagcaggg
     2521 gcgacagcag ccgtgcgaaa gcctccggca gccgcgcgcg ccatagcggg cgctcgcggt
     2581 cggcgcgcac ccgcggtgcc tgcgcgaatt cgccctcgat cagtttcgcg aggtcgccag
     2641 ccacgccggc atcgcggatc agcaggcaga cctcgaagtt gaggcgaaag ctgcggtggt
     2701 cgaaattcgc gctgccgacc agggcgagct cgtcgtccac cagcagcgcc ttggtgtgca
     2761 gcatgcgcgg gccgtattcg tagaccttga ccccggcttc cagcaggctg tcgaagtagg
     2821 agcgtgcggc gtaggtgacc agggtgctgt cgctggtctt cggcaccagc aggcgcacgt
     2881 cgagcccgcc gaacccggcc gaggtgagcg ccatcatcgc cgcctcgccg ggcacgaagt
     2941 acggggtgac cagccacacc cgccgcttcg ccgcgtggat cgcgccgacg tgcaggcggt
     3001 ggatcgcctc ccagtcggag tccgggccgg aggtcagtac ctgcgcatgg accggcccgt
     3061 gcgcgggtgg cggcatcgcc cgcgccacct cgccgatgaa gtcgcggctg ccggtggcgt
     3121 acacccagtc ctcgacgaac accagctgca gggcgcgcac gctgtcgccc tcgatccgca
     3181 ggtgcaggtc gcggtaggcg tctgcgcgca ggcgctcgtt ctcctcgtcg gtgatgttga
     3241 tgccgccggc gaagccgacc cggccatcga ccaccacgat cttgcggtgg gtacgcatgt
     3301 tcagccacgg ccgcttccac acgtgcccga agcgcatcgg atggaaccag gccagttcgc
     3361 cgccggctgc ccgcaggtcg gcgaagaacg catccgcggt gccggcgctg cccaccgcat
     3421 ccagcagcag gcgcaccttc accccggcgc gggcgcgttc gaccagcgca tcgcgcaggg
     3481 cggtgccgct gcggtcgggg tggtagatgt agtactcgag gtggacgtgg tcgcgggcac
     3541 cacgcactgc gtccagcagc gcgtcgtagg tcgccgcacc gtcgatcagc aggtccacgc
     3601 gttgcgcggt ggtcggcggc agcccggtgg tcgcctgccc gagtcgcgcc agttccactg
     3661 cctcgtgctc accttcgcct ggtggtcccg gcatgcccac ccgcgcccgc gagcgccgca
     3721 gccggtggcg gcggatcttc tgcggaccga acacgtggta gacgaagaag ccgatgtagg
     3781 gcagggcggc caggctgacc agccagctga tcgtcgccgc cggctcgcgc ttctgcagca
     3841 cgatccacac ccccagtccg agcaggtacg caagccagcc caggccgacg tagaggccga
     3901 tgtgcgggat cgcccacagg gcctcccagg acgacagcag cgtgggcatt cggtctccgg
     3961 tggtcgcgcc aggcgcgcgc gctacgacca cgacagggta gggcgcgcgg ccgcgaatgt
     4021 cgcgtgacgg gccggccgca gccgggcgcc ggctacgggc ggccgccgtg ttcgccaggc
     4081 tcagccggac agcggcagca gcggaccccc gagccacagc cagcgcgcca tccacgcgct
     4141 gaccagggcg atgaccacgg tcagcggcag gccgatgcgc aggaaatcgc cgaaccggta
     4201 ctggcccggc ccgaggatca gcaggttgcc gtggtggccg atcggggtca ggaacgcgac
     4261 caccgccccc agcgcggtgc acaccacgaa gggcgtcggc ggaaggtcca gggtgcgtgc
     4321 cagcgagatc gcgaccggcc ccagcaacac ggtggtggcc gcgtccgaca tcacctgggt
     4381 gagcaacgcc gcggcggtga acatcaccag caggatcggc agcgccggcc atcccgccac
     4441 catgtgcgac agcccgcgcg ccagcaggtc ggcggtgccg gtctgttcca tcgcgatgcc
     4501 gagcgggatc accccggcga tcatcacgaa gatgcgtacg tcgatgcccc ggtaggcctg
     4561 ctggatgtcg acgcagcggg tcgagaccat cgccaccgcg cccatcagga acgccagcgg
     4621 ggcggacagc cactcggtgg cggcggcgag gatggtgacg ccgaggatca gcaacgccaa
     4681 cggcgcatgc acgcggcgct tggcctcgcc ggcaaacggc accagcatca ggaagccatg
     4741 gtgcgccgcg aggtcggaga accgcgacgg gcgcccgtac agcaccagca ggtcgccctc
     4801 gcgcaggcgc gcgtccgaca gccggtcggc catgtcgccc tcgcgccgcc acaggccggc
     4861 gatcactgcg tggaactggc gggcgaagtc gagctcgcgg atgctgcggc cgatgaattc
     4921 agagcccggg gcgaccaccg cctgcaccag ctgcggcgcg ccctcgccgc tgacggtgtc
     4981 gttgaagcgc acgatcgcgt tgaggtccag gccggggtcg tcgtgcagcg acagcagctc
     5041 gtcggcggag gcctcgacca gcagcacgtc gccggccagc agcggactgg tcgcggtgag
     5101 gtcgtcgcgc cgcacgccat cgcgcagcca gccgagcagg cggaagcgcg cgcccagcgc
     5161 cttgtgcagt tcgctgagcg ggcgggtggt ccagcgcccg tccttcacca ccagcagttc
     5221 ggtgcggtag cggtccagcc gcaggtagtc gtcgtcgccg tgggtgccgc tgcgcttggg
     5281 cagcagccag cgcgcgccga gcatgtagct ggtcccgacc agcaccaggg cgatgccgat
     5341 cggggtgatc gagaagatcc cgaggccgtc ggtcccggcg cgttgcagca tgtcgctggc
     5401 gagcaggaac gccggcgcgc tgaacagggt cagggtggtg cccagcgacg ccgccagcga
     5461 catcggcatc agcagccgcg aagccggcaa ctggcggtcg cgcgcatgcc gcagcagcag
     5521 cggcagcatc atcgcggtca ccatcacgtg gtgggtgaac gccgccagca gggccaccgc
     5581 cggcatcacc accgcgatcg tgcgccactc gtggacgccg gcggcgcgcc cgatccacgc
     5641 ccccagccgt tcgctgatgc ccgtggcggc gagcgccccg gaaatcacga agaccgcggc
     5701 gacgatgatc gccggctcgc tggcgaagcc ggacagcgcc tgcttgctgt ccagcacgcc
     5761 ggtcagcacc agcgccagca ggatcagcat cgcggtgacg tcgactcgaa gcctctcgga
     5821 aatgaacagg tacagcccga cggccaggat caggccgaag gcgatctgcg aggacagcag
     5881 cggataggtc gagagcatcc ggccattgtg cccgctcgcg gcgggcgcgg gcatcgtcgc
     5941 ggccatgtgc acatgcgctt tacgacgcag acgcctggat tccgcccacc caggaccgac
     6001 cgcccatgcc ccagccgcag gccgcgaagt gctgcaggcg atccgcgcca accgctacat
     6061 cagaagccgg tggaccacga ccgcttcgtc gagcgggtgc gccggatcgg gctgtactgg
     6121 ctggccctgg acccgccgcc gggaaagccc tggaccggga tcaggcggcg tccgccgcgt
     6181 ggtggtagcg gaccgcggcc tcgacctccg ccttcgaccc caggaacacc ggcacccgct
     6241 ggtgcaggcc ggtcggctgc acctccagca tgcgctggcg gccggtgctg gcggcgccgc
     6301 cggcctgttc gaccagcagc gccatcgggt tggcttcgta catcagccgc agcttgccgc
     6361 ccttgggcgc gcacttgctg tccagcggat agctgaagat gccgccacgg gtgaggatgc
     6421 gatggacgtc ggcgaccatg ctcgccaccc agcgcatgtt gaagtccttg ccgcgcgggc
//
LOCUS       DAORUK010000121         6368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_14299_length_6368_cov_15.852843, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000121 DAORUK010000000
VERSION     DAORUK010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14299_length_6368_cov_15.852843"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..966)
                     /locus_tag="PK818_08235"
     CDS             complement(76..966)
                     /locus_tag="PK818_08235"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQY82755.1"
                     /translation="MPRALVNGVELDYEIFGPEQGEPLLLVMGLAMQRVAWPAALLDA
                     LVARGFRCITVDNRDVGFSTRYEDIRAPSLPTSIAARLFGLRPRLPYRLADLADDDMA
                     LLTHLGIHAAHVAGISMGGMVAQHVAGHHGERVRSLTLMATSSGRLGLPPPRPKVLRL
                     MMSRPTGRVSVDEGVAYVTRLYRTIGSPAWPMSPADMEGRARIGVMRAAAGTGVTRQL
                     AAIIADGDRSPMLRRLKVPTLVLHGSADPMVPIAHGIDLARVIPGARFESIEGWGHDL
                     PDALAPVFADHIAAQARKAR"
     gene            complement(966..1784)
                     /locus_tag="PK818_08240"
     CDS             complement(966..1784)
                     /locus_tag="PK818_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQY82756.1"
                     /translation="MSKRKPRRSASAAPTPAPAHAHLRAPGWRELALEARAPWEFAGG
                     LLSWWMAHRLPRGDGQPVVVFPGLAASDTSTLLLRQVLKRLGYVPYGWGQGFNAGPRH
                     GVLKNCIERIEDIHVRHGRSVSLVGWSLGGLYAREMAKLAPEQVRQVITMGSPFAGHP
                     RATNAWPLFRLLSGHHEVDPHLQARLREPPPRPTTSIFSRSDGVVAWQCSVQEAEHPH
                     QNENVELSASHFGMGFNPMVLYVIADRLAQGENAWRPFEREGLRRLFYPEPVNG"
     gene            complement(1781..3250)
                     /locus_tag="PK818_08245"
     CDS             complement(1781..3250)
                     /locus_tag="PK818_08245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQY82757.1"
                     /translation="MALKALSGLDAGFLYIETPETLMHVGSLCSFERPADLRGAFLPR
                     VRKHIAGRMHLAPIFTRRLALMPLDLGHPVWVEAGKVDLDYHIQGARLPKPGSERQLE
                     ALVAKLHAQPLDRERPLWQFTVIEGLADGQVALYSKVHHAALDGQAGVALAQAILDLG
                     PVPREVPPPHEAHRARAPSRRLMLGTLFSNSLAQYVKLVRSVPSAVRAAAGAAVSLTD
                     KIVAKRGLPDDLMAPRTRFNTQIGASRAFATCTLPLRDFKAVAAACEATINDLLLATC
                     SGALRQYLAGHHELPKRSLVAAVPLSLRESGESAAGNQVTMLPCPLGTDQKTPRARLA
                     AIQAGMTKVKGTKTSFRDLIPTDYPSLGSPWLMSGLAQLYARTRLADRIPLPANLVIS
                     NVPGPPVTLYLAGARMTTYFPVSIVVHGLALNITVHSYDGKLHLGLIACRKNVPDLPK
                     LVAALYESFDELKAMAASELSAATPRPGARPAAKRRKPR"
     gene            3524..5206
                     /locus_tag="PK818_08250"
     CDS             3524..5206
                     /locus_tag="PK818_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82758.1"
                     /translation="MAILSTLPDRIRRFPIVLLALVACGSASAFEVIIRIGDPAPQIG
                     TSGYTIESFVEPPSIISGSGPDSGYSVFHVTIAGPGVDANNSHVIYRWSRGVGLVRVA
                     RGGDSIVFPDGTRILTILESPLASVYGAVAYTSQLDDGRRGLVLWRPGVPSLGIARVG
                     QSVVIPCDALAGGPCGPVAGTLGNLPTFADRKGMAFVSEFDLLGILRHQFAFRGVVQD
                     TGGFGTPDALWYRELRENDIAGTLLNLSNSLRKAEGLGANNIYFDDFTDLSFCTASYL
                     LGLATVSRDAPFVVYRFRETGVHTPLGRDYAPDMVPRRDLHCVGATGSYVGGLSNAPF
                     DGVVANKGAWLVDTATAVQSQVYSNTVTPAPAPAGATLGVPLGQAPDILRGGGSVFAA
                     RLIGSAANGRVGIYRGAPGSTLVPLIHETESLPGQSQVVDRIWSVAVDVFGLALVHLR
                     LVDQRQAIRLVGTLPGDSRAVLASGDSITVAPGDVRTLTSFWLLGGTFGTDPVVTGYG
                     GDGLQGAFARNGDAVLVAYYAASSGAPAGAAMVAAQLSVPLFADGFEDPPPT"
     gene            complement(5285..6364)
                     /locus_tag="PK818_08255"
     CDS             complement(5285..6364)
                     /locus_tag="PK818_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY82759.1"
                     /translation="MNSLRRLLAIVRKELMQLRRDRLTFAMIVGIPTIQLLLFGYAIN
                     MDVRGLDAAIVDQADTHASRALAQELANSQVITVRYRMADPAPVQELIRQGRISVALV
                     LPPDLDRRLARRELPAMQLIVDGSDPVMLSAAQQLASLPLDGSARPALQVLNLYNPER
                     RSAINTVPGLVGVILTMTMTLFTAVAIVRERERGNLELLITTPVQPLELMLGKVVPFI
                     GIGLVQVTLVLGLGVLIFGVPVRGALLDFYLAALAYIVAALSLGVFISTMTRTQFQAM
                     QGAFFLFLPQILLSGFMFPYAGMPRPAQWIAEFLPLTHFIRLVRGILLRGASLADLWL
                     PLVTLLGFSVLMLTFAVGRFRKRLD"
BASE COUNT          947 a         2110 c         2348 g          963 t
ORIGIN      
        1 attcccgctt gcgcgggaat gacgccgggt ggtggaggcg atacgccgcg tgggaaggcg
       61 gttcgggttc ggcgctcagc gcgccttgcg ggcctgcgcg gcgatgtggt cggcgaagac
      121 cggggcgagg gcgtcgggga ggtcgtggcc ccagccttcg atggattcga agcgggcgcc
      181 ggggatcacg cgggcgaggt cgatgccgtg ggcgatcggg accatcgggt cggcgctgcc
      241 gtgcaggacc agggtcggca ccttgaggcg gcgcagcatc gggctgcggt cgccatcggc
      301 gatgatcgcg gcgagctggc gggtgacgcc ggtgccggcg gcggcgcgca tcacgccgat
      361 gcgggcacgg ccttccatgt ccgcgggcga catcggccag gccgggctgc cgatggtgcg
      421 gtacaggcgc gtgacgtagg ccacgccctc gtcgacgctg acccggccgg tgggccgcga
      481 catcatcaac cgcagcacct tgggccgcgg cgggggcagg ccgaggcggc cgctgctggt
      541 cgccatcagg gtcaggctgc gcactcgctc gccgtggtgg ccggcgacgt gctgcgccac
      601 catgccgccc atcgagatgc cggcgacgtg cgcggcgtgg atcccgaggt gcgtgagcag
      661 cgccatgtcg tcgtcggcga ggtcggcgag gcgatagggc aggcgcggcc gcaggccgaa
      721 caggcgcgcc gcgatcgagg tcggcagcga gggcgcgcgg atatcctcgt agcgggtcga
      781 gaagccgacg tcgcggttgt cgaccgtgat gcagcggaag ccgcgcgcga ccagcgcatc
      841 gagcagcgcg gccggccagg ccacgcgttg catggccagg cccatgacca gcagcagcgg
      901 ctcgccctgc tcgggcccga agatctcgta gtcgagctcg acgccgttga ccagggcccg
      961 cggcatcagc cgttcaccgg ctccgggtag aacaggcgcc gcaggccctc gcgctcgaac
     1021 gggcgccagg cgttctcgcc ctgcgccagg cggtcggcga tcacgtacag caccatcggg
     1081 ttgaagccca tgccgaagtg gctggccgac agctccacgt tctcgttctg gtgcggatgc
     1141 tcggcttcct gcacgctgca ttgccacgcc accacgccgt cgctgcgact gaagatcgag
     1201 gtcgtcggtc gcggcggcgg ttcgcgcagc cgcgcctgca ggtgcggatc gacctcgtgg
     1261 tgtcccgaaa gcaggcggaa cagcggccag gcgttggtcg cgcgcggatg cccggcgaag
     1321 ggcgagccca tggtgatgac ctggcgcacc tgctcgggcg cgagcttggc catctcgcgc
     1381 gcgtagaggc cgcccaggct ccagccgacc aggctgacgc tgcgcccgtg gcgcacgtgg
     1441 atgtcctcga tgcgctcgat gcagttcttg agcacgccgt ggcgcggacc ggcgttgaag
     1501 ccctggcccc aaccgtaggg aacgtagccc aggcgcttga gcacctggcg cagcagcagc
     1561 gtgctggtgt cgctggcggc gaggccgggg aataccacca ccggttgacc gtcgccgcgc
     1621 ggcagccggt gcgccatcca ccagctcagg aggccgccgg cgaactccca gggggcgcgc
     1681 gcctcgagcg cgagttcgcg ccagccgggc gcgcgcaaat gcgcatgggc cggcgccggc
     1741 gtcggcgcag cggatgcgct gcgtcgcggc ttgcgcttgc tcaccgcggc ttgcggcgct
     1801 tggcggcggg ccgagcgccg ggacgcggcg tggccgcgga caattcactc gccgccatcg
     1861 ccttgagttc gtcgaaggat tcgtacagcg cggccaccag cttcggcagg tcgggcacgt
     1921 tcttgcggca ggcgatgagg ccgaggtgga gcttgccgtc gtagctgtgc accgtgatgt
     1981 tgagcgccag cccgtggacc acgatcgaga ccgggaagta cgtggtcatg cgcgcgccgg
     2041 ccaggtacag cgtcaccggt ggtccgggca cgttggagat cacgaggttg gccggcaagg
     2101 ggatgcggtc ggccaggcgc gtgcgcgcat acagttgcgc cagcccggac atcagccagg
     2161 gcgagcccag cgagggatag tccgtgggaa tcaggtcgcg gaacgaggtc ttggtcccct
     2221 tgaccttggt catgccggcc tggatcgcgg ccaggcgcgc gcgcggcgtc ttctggtcgg
     2281 tgcccagcgg gcagggcagc atggtcacct ggttgccggc ggcgctttcg cccgattcgc
     2341 gcagcgacag cggcaccgcc gccaccagcg agcgcttggg cagttcgtgg tgcccggcaa
     2401 ggtactgccg cagcgcgccc gagcaggtcg ccagcagcag gtcgttgatg gtcgcctcgc
     2461 aggccgccgc caccgccttg aaatcgcgca gcggcagggt gcaggtggcg aaggcgcggc
     2521 tggcgccgat ctgggtgttg aagcgcgtgc gcggcgccat caggtcgtcg ggcaggccgc
     2581 gcttggccac gatcttgtcg gtcaatgaaa ccgccgcgcc ggccgcagcg cgcaccgcgc
     2641 tcggcaccga acgcaccagc ttcacgtact gcgccagcga gttcgagaac agcgtgccca
     2701 gcatcagccg gcggctgggt gcgcgcgcgc gatgcgcctc gtgcggcggc gggacctcgc
     2761 gcggcaccgg cccgaggtcg aggatcgcct gcgccagcgc gaccccggcc tgtccgtcga
     2821 gcgcggcgtg gtgcaccttc gagtacagcg ccacctggcc gtcggcgagg ccctcgatca
     2881 ccgtgaactg ccacagcggg cgctcgcgat ccagcggctg cgcatgcaac ttcgccacca
     2941 gcgcctcgag ctggcgctcg ctgcccggct tgggcaggcg cgcgccctgg atgtggtagt
     3001 cgagatcgac cttgccggcc tcgacccata ccgggtggcc aaggtccagc ggcatcaggg
     3061 cgaggcgacg ggtgaaaatc ggcgcgaggt gcatgcgccc ggcgatgtgc ttgcgcacgc
     3121 gcggcaggaa agcgccgcgc aggtccgccg gacgctcgaa gctgcacagg ctgcccacgt
     3181 gcatcagcgt ttccggcgtc tctatgtaga ggaagccggc gtcgagtccg ctcagtgcct
     3241 tcagggccat gccgcgcctt ggttcatgca gccgggcgga gcatggcgga ggcgggggcc
     3301 gaggtcaaag gcggtgtgct catgttgcag cgcagcttgg acgaacgtcc agttgcggcg
     3361 ggagttctgc tgcgctgcaa gattgccgac ctcggaaatg aacctgggcg cttcgcggta
     3421 ggcggccaag ccaagcccgt ccgccccggc ttgtcgcgat gcggttggtt tcgtcgcttg
     3481 gaggctatca ggggccgctc gggcccatgc aggaggtacc gccatggcga tcctgtccac
     3541 gctgccggac cgcattcgcc gcttcccgat cgtgctgctg gcgctggtag cctgcggttc
     3601 cgcgagtgcg ttcgaggtga tcatccggat cggcgatccg gcgccgcaga tcggcaccag
     3661 cggctacacc atcgagagct tcgtcgagcc gccatcgatc atcagcggat ctggcccgga
     3721 ttcaggctat tccgtattcc acgtcaccat cgccggtccc ggcgtggatg cgaacaacag
     3781 ccacgtcatc taccgctggt cgcgtggcgt cggcctggtg cgggtcgcac gcggtggcga
     3841 cagcatcgtg ttcccggacg gcacccggat cctcaccatc ctcgaatcgc ctctggccag
     3901 cgtttacggc gccgtggcct acacctcgca actggacgac gggcgacgcg gcctggtgct
     3961 gtggcgcccc ggcgtgccgt ccttgggtat cgcgcgggtc gggcaaagcg tggtgatccc
     4021 ctgcgacgct ctcgccggcg gtccctgcgg gccggtggcg ggaaccctgg gcaacctgcc
     4081 gacctttgcc gatcgcaagg gcatggcctt cgtttcggaa ttcgacctgc tgggcatcct
     4141 ccgtcaccaa ttcgcattcc gcggcgtggt ccaggacacc ggtggattcg gcacgccgga
     4201 tgcgctctgg taccgcgaac tgcgtgagaa tgacatcgca ggaacgctgc tcaacctgag
     4261 caactcgctg cgcaaggccg aaggcctggg cgccaacaac atctacttcg acgacttcac
     4321 cgacctcagc ttctgcactg ccagttacct gctgggcctg gcgaccgtgt cccgggatgc
     4381 gcccttcgtg gtctaccggt tcagggaaac cggcgtgcac acgccgctcg gccgggacta
     4441 cgcacccgac atggtcccgc gacgcgacct gcattgcgtt ggcgcgacgg gcagctacgt
     4501 gggtggactt tccaacgctc cgttcgatgg ggtcgtcgcc aacaagggcg catggctggt
     4561 agacaccgca accgcggtgc aatcgcaggt ctattcgaac acggtaacgc cggcgcccgc
     4621 gccggcgggc gcgaccctgg gtgtaccgct gggtcaggcg ccggacatcc tccgaggggg
     4681 cggatcggtg ttcgcagccc ggctgatcgg ctccgcagcc aacggtcgcg ttggcatcta
     4741 tcgcggcgcg ccgggatcca cgctggtgcc gctcatccat gagaccgaga gtttgcctgg
     4801 ccagtcccag gtggtggacc ggatatggag cgtggcggtg gacgtattcg gcctggccct
     4861 cgtgcacctg cgcctggtcg accagcgcca ggcgataaga ctggtcggca cgctgcccgg
     4921 cgactcacgc gcagtactcg cttcgggcga ttcgatcacg gtggcgccgg gcgacgtgcg
     4981 taccctgacc tcgttctggc tgctaggcgg cacgttcggt actgatccgg tcgtcaccgg
     5041 ctacggcggg gacgggctgc aaggcgcctt cgcacggaac ggcgatgcgg tgctggtggc
     5101 ctactacgcg gcatcgtccg gcgcgccggc cggtgctgcg atggtcgccg cacagctctc
     5161 ggtgccgctg ttcgccgacg gcttcgagga tccgccgccg acctgaggcg gcggggtcgc
     5221 ggtcctggcg ttgcaagaat ggggactgag gggattaagg cattaatccc ctcggcccca
     5281 tcttctaatc gaggcgcttg cggaatcggc cgacggcgaa ggtcagcatc aggaccgaga
     5341 accccagcag ggtcaccagc ggcagccaca ggtcggcgag gctggccccg cgcaacagga
     5401 tcccgcgcac caggcggatg aagtgggtca gtggcaggaa ctcggcgatc cattgcgcag
     5461 ggcgcggcat gccggcatag gggaacatga agccggagag caggatctgc ggcaggaaca
     5521 ggaagaaggc gccctgcatg gcctggaact gggtcctggt catggtcgag atgaacacgc
     5581 ccagcgagag cgcggccacg atgtaggcca gcgcggcgag gtagaaatcc agcagcgcgc
     5641 cgcgcaccgg cacgccgaag atcaggaccc cgaggcccag caccagcgtg acctggacca
     5701 ggccgatgcc gatgaagggc acgaccttgc ccagcatcag ttccagcggc tgcaccgggg
     5761 tcgtgatcag cagttccagg ttcccgcgct cgcgctcgcg cacgatcgcg accgcggtga
     5821 acagcgtcat ggtcatggtc aggatcacgc cgaccagccc gggcacggtg ttgatcgccg
     5881 agcggcgctc ggggttgtag aggttgagga cctgcagcgc cggccgcgca ctgccgtcga
     5941 gcggcagcga ggcgagttgc tgcgcggcgc tcagcatcac cggatcggaa ccgtcgacga
     6001 tgagctgcat cgccggcagc tcgcggcggg cgaggcggcg gtcgaggtcc ggcggcagca
     6061 ccagcgcgac gctgatgcgg ccctggcgga tcagttcctg caccggcgcc gggtcggcca
     6121 tgcggtagcg caccgtgatg acctggctgt tggccagttc ctgcgccagc gcgcgcgagg
     6181 catgggtgtc ggcctggtcc acgatcgcgg cgtcgaggcc gcgcacgtcc atgttgatgg
     6241 cgtagccgaa cagcagcagc tggatggtgg ggatgcccac gatcatggcg aaggtgaggc
     6301 gatcgcggcg cagctgcatc agctccttgc gcacgatggc gagcaggcgg cgcagggagt
     6361 tcatcggc
//
LOCUS       DAORUK010000122         6318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_14475_length_6318_cov_21.834903, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000122 DAORUK010000000
VERSION     DAORUK010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14475_length_6318_cov_21.834903"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="PK818_08260"
     CDS             complement(<1..1408)
                     /locus_tag="PK818_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 1"
                     /protein_id="HQY82760.1"
                     /translation="MSATHPTAVAASDKHGHDDHGHKQGFVERWFFSTNHKDIGTLYL
                     VFSFIMFIIGAAFSVVIRTELATPGLQHVSPEFFNQMTTLHALVMIFGGVMPAFVGLA
                     NWMVPLQIGAPDMALPRMNNWSFWIMPFAFSLLLMTLFLPGGGPAGGWTLYPPLSLQG
                     GNNVAFVIFAIHMMGISSIMGAINIIATILNMRAPGVDLLKMPIFCWTWLITAFLLIA
                     VMPVLAGAVTMLLTDKFFATSFFNAAGGGDPVMFQHIFWFFGHPEVYIMILPAFGIAS
                     EIIPTFARKPLFGYQAMVYATAAIAFLSFIVWAHHMFTVGMPLGGEIYFMFATMLISI
                     PTGVKVFNWVTTMWRGSISFESPMLWAVAFVILFTIGGFSGLMLAIVPADFQYHDTYF
                     VVAHFHYVLVTGALFAIIAAVYYWWPKWTGRMYNETFAKIHFWWSVVFVNLLFFPQHF
                     LGLAGMPRRIPDYNVVFAD"
     gene            complement(1438..2361)
                     /gene="coxB"
                     /locus_tag="PK818_08265"
     CDS             complement(1438..2361)
                     /gene="coxB"
                     /locus_tag="PK818_08265"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HQY82761.1"
                     /translation="MLAQAQDAANHADPHRWQLNMAPGVTPTSQAAYDAHMIVLWICV
                     VIGILVFGAMAYAMFRFRKSKGAVPDKEFTHSTRLEIIWTAVPVLILIGLAFPATSGL
                     MRMYDTRDAEMTVKVTGYQWMWKYEYLGEGVEFTSRLDRRSEEVRQSGVDARTANHPH
                     YLLDVDNALVLPVGTKVRFVLTADDVIHAWWVPALGWKQDAIPGLVNEAWAQIEKPGV
                     YRGQCAELCGKDHGFMPIVVKAVSKPEFDTWLAAEKARLAPPAAAEADAAAPAPAAGA
                     APGTEANDAAPAEADAAPQAEQAPADAAAAA"
     gene            2649..5864
                     /gene="putA"
                     /locus_tag="PK818_08270"
     CDS             2649..5864
                     /gene="putA"
                     /locus_tag="PK818_08270"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HQY82762.1"
                     /translation="MSNQASTSQTPAPILSPELAPVPDPGRAAITAGWILDEATHVRE
                     MLALAALPEREHAAAQAVAADLVRRVRARARDQGVIEAFMRQYDLGSEEGVLLMCVAE
                     ALLRIPDQATADALIRDKLGDANWKRHLGQSDSVLVNASTWGLLLTGKLVDLADDTKR
                     DAHGAFQRLVGRAGEPVIRLAVRQAMKIMGHQFVLGRTIDEALARSRKGDNAAYRYSF
                     DMLGEGALTTRDALRYQDAYRSAIHAIGQTGPGGSWVGSDVFAAPSISVKLSALHPRY
                     EHAKRARVLAELGERLLELAQLARRYGIGLTIDAEETDRLELSLDLIFQVLAEPSLSG
                     WNGFGIVVQAYQKRAPFVIDYIAHMAAKLDRRIPVRLVKGAYWDAEIKRAQMDGQAGY
                     PLFTRKQNTDVSYLANARRLLDAGNAIYPMFATHNAQTIATVHRMARAMRGRRDFEFQ
                     KLHGMGDDLYAEVIPADRLDVPCRVYAPVGSHEDLLPYLVRRLLENGANSSFVNRITD
                     ESIPVEELVRDPVEFVSALEHIQHPRIPLPVNLYRSHHQHRDNSMGINLANDDQLREL
                     AAALNTSGAGSWAAGPLVPGAANAGDRIAVSNPADRREVVGHWQAADAATVEQALRNA
                     SAAHAGWDATPAGSRAAILEHAASLLEARMPAYMAMCTKEAGKTLADGIAEVREAVDF
                     LRYYALQAREHFAPVALPGPTGEANQLQLAGRGVFACISPWNFPLAIFLGQVAAALAA
                     GNTVIAKPAEQTNLIGFAAVRLLHEAGIPQDVLQFVPGDGATVGAALTRDPRVAGVCF
                     TGSTETARLINRALASREAGPIATLIAETGGQNAMIADSSSLPEQVVKDALGSAFTSA
                     GQRCSAARVLLVQEDIADKVIGMLAGAMAELTVGDPGLPSTDVGPVIDEDARAILVEH
                     AARMDAEARPIAKAALGAGCEHGTFFAPAAWELRSIAQLDREKFGPALHVVRWKAEEL
                     DAVIDTINATGYGLTLGIHSRIDATIDRIVSRAKVGNIYVNRNQIGAVVGVQPFGGQG
                     LSGTGPKAGGPHYLPRFATEKTVTVNTTAAGGNASLLTLGD"
     gene            complement(5958..>6318)
                     /locus_tag="PK818_08275"
     CDS             complement(5958..>6318)
                     /locus_tag="PK818_08275"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HQY82763.1"
                     /translation="GTVVGSEDFSWEDTDFFSVGMEYDLSDAFTLRGGIGWDESPTND
                     VTRTPRLPDNDRMLYSVGMSWAFSDNLTVDAAYQRIEIDDPTIDLGVHVPSDLSTLHG
                     TFGGYANLFGVSMQYRF"
BASE COUNT         1068 a         2203 c         2133 g          914 t
ORIGIN      
        1 agtccgcgaa caccacgttg tagtccggga tgcggcgcgg catgccggcc aggcccagga
       61 agtgctgcgg gaagaacagc aggttgacga acaccaccga ccaccagaag tggatcttcg
      121 cgaaggtctc gttgtacatg cgcccggtcc acttcggcca ccagtagtac accgcggcga
      181 tgatcgcgaa cagcgcgccg gtcaccagca cgtagtggaa gtgggcgacc acgaagtagg
      241 tgtcgtggta ctggaagtcc gccggcacga tggccagcat caggccggag aagccgccga
      301 tggtgaacag gatcacgaag gccaccgccc acagcatcgg cgactcgaag ctgatcgagc
      361 cgcgccacat ggtggtgacc cagttgaaca ccttcacgcc ggtcgggatc gagatcagca
      421 tcgtcgcgaa catgaagtag atctcgccgc ccagcggcat gcccaccgtg aacatgtggt
      481 gcgcccacac gatgaacgac aggaacgcga tcgccgcggt ggcgtacacc atcgcctggt
      541 agccgaacag cggcttgcgg gcgaaggtcg ggatgatctc gctggcgatg ccgaacgccg
      601 gcaggatcat gatgtagacc tccgggtgcc cgaagaacca gaagatgtgc tggaacatca
      661 ccgggtcgcc gccgccggcc gcgttgaaga agctggtggc gaagaacttg tcggtgagca
      721 gcatggtcac cgcgccggcc agcaccggca tcaccgcgat cagcaggaac gcggtgatca
      781 gccaggtcca gcagaagatc ggcatcttca gcaggtccac gcccggggcg cgcatgttga
      841 ggatggtggc gatgatgttg atcgcgccca tgatcgagct gatgcccatc atgtggatgg
      901 cgaagatcac gaaggccacg ttgttgccgc cctgcagcga cagcggcggg tacagcgtcc
      961 agccaccggc aggcccgccg cccggcagga acagggtcat cagcagcagg ctgaaggcga
     1021 acggcatgat ccagaacgac cagttgttca tgcgcggcag cgccatgtcc ggcgcgccga
     1081 tctgcagcgg caccatccag ttggccaggc ccacgaaggc cggcatcacc ccgccgaaga
     1141 tcatcaccag cgcatgcagg gtggtcatct ggttgaagaa ctcggggctg acgtgctgca
     1201 ggcccggtgt ggccagttcg gtgcggatca ccaccgagaa cgccgcgccg atgatgaaca
     1261 tgatgaagct gaacaccagg tacagggtgc cgatgtcctt gtggttcgtg gagaagaacc
     1321 agcgctcgac gaagccctgc ttgtggccgt ggtcgtcgtg accgtgcttg tcggaggcag
     1381 cgacggcggt gggatgggtg gcggacatgc gctggacctc gggattcggt gcgtggatca
     1441 agcggccgcc gcggcgtcag ccggggcttg ttcggcctgc ggggcggcat cggcctcggc
     1501 cggtgctgca tcgtttgctt cggtgccggg cgccgccccg gcggcgggcg cgggtgccgc
     1561 ggcgtcggct tcggcggccg ccggtggcgc cagcctggcc ttctcggccg caagccacgt
     1621 atcgaattcg ggcttgctca ccgccttcac cacgatcggc atgaagccat ggtccttgcc
     1681 gcacaattcg gcgcactggc cgcggtacac accgggcttt tcgatctgcg cccacgcctc
     1741 gttgaccagg cccgggatcg cgtcctgctt ccagcccagc gccggcaccc accaggcgtg
     1801 gatgacgtcg tcggcggtca gcacgaagcg gaccttggtc ccgaccggca gcaccagcgc
     1861 gttgtcgaca tccagcaggt agtgcgggtg gttggcggta cgcgcgtcca ccccgctctg
     1921 gcggacttcc tcgctcctgc ggtcgagccg gctggtgaac tccacccctt cgcccaggta
     1981 ttcgtacttc cacatccact ggtagccggt gaccttgacg gtcatctcgg catcgcgggt
     2041 gtcgtacatg cgcatcaggc cgctggtggc cgggaacgcc agcccgatca ggatcagcac
     2101 cggcaccgcg gtccagatga tctccagcct ggtgctgtgg gtgaactcct tgtccggcac
     2161 cgcgcccttg gacttgcgga acctgaacat ggcataggcc atcgccccga acacgaggat
     2221 gccgatcacc acgcagatcc acagcacgat catgtgcgcg tcgtaggcgg cctgcgaggt
     2281 cggggtgacc ccgggcgcca tgttgagctg ccagcggtgc ggatccgcat ggttcgcggc
     2341 gtcctgcgcc tgcgccagca tcggcagcgc gagtgccgcc gccaacgccc acaacctggg
     2401 gacgcacgtc ctgaatcgac cgggtttgat cacatcgagc cccattggga tgcctggaga
     2461 gcggacgggc cgatccccgg gtcgcttgac ccagatcaac cccgaaccga acgcgcgatg
     2521 ttaaaggccg cgcgctcggg cggcaacccg caaacggagc ggagcccgcc aacggtgccc
     2581 cgggcgaccc accgcactac actaggcgac cgcatttccc ttgggcccgc aggcggccag
     2641 acggcgcgat gagcaaccaa gctagcactt cccagacccc cgcgccgatc ctttcgccgg
     2701 aactcgcccc cgtgccggat ccgggacgcg cggcgatcac cgccggctgg atcctcgacg
     2761 aggccaccca tgtgcgcgaa atgctggcac tggcggcatt gccggagcgg gagcacgccg
     2821 ccgcgcaggc ggtggccgcc gacctggtgc gccgggtccg cgcccgcgcc cgcgaccagg
     2881 gcgtgatcga ggccttcatg cgccagtacg accttggcag cgaggaaggc gtgctgctga
     2941 tgtgcgtggc cgaggcattg ctgcgcatcc ccgaccaggc caccgccgac gcactgatcc
     3001 gcgacaagct gggcgatgcc aactggaagc gccacctcgg ccagtccgat tcggtgctgg
     3061 tcaacgcctc cacctggggc ctgctgctga ccggcaagct ggtggacctg gccgacgaca
     3121 ccaagcgcga cgcccacggc gcgttccagc gcctggtcgg acgcgccggc gaaccggtga
     3181 tccggttggc ggtgcgccag gcgatgaaga tcatgggcca ccagttcgtg ctggggcgga
     3241 ccatcgacga agcgctggcg cgcagccgca agggcgacaa cgccgcttac cgctactcct
     3301 tcgacatgct gggcgaaggc gcactgacca ccagggacgc gctgcgctac caggacgcct
     3361 accgctccgc catccatgcg atcggccaga ccggccccgg cggcagctgg gtcggcagcg
     3421 acgtgttcgc cgcgcccagc atctcggtca agctctccgc gctgcacccg cgctacgagc
     3481 acgccaagcg cgcgcgggtg ctggccgagc tgggcgagcg cctgctggag ctggcgcagc
     3541 tggcgcgccg ctacgggatc ggcctgacca tcgacgccga ggaaaccgac cgcctggagc
     3601 tgtcgctgga cctgatcttc caggtcctgg ccgagccctc gctgagtggc tggaacggct
     3661 tcggcatcgt ggtgcaggcc taccagaagc gcgcgccgtt cgtgatcgat tacatcgccc
     3721 acatggccgc caagctggat cgccggatcc cggtgcgcct ggtcaagggc gcgtactggg
     3781 atgccgagat caagcgcgcg cagatggatg gccaggccgg ctatccgctg ttcacccgca
     3841 agcagaacac cgatgtcagc tacctggcca acgcgcgccg cctgctggac gccggcaatg
     3901 cgatctaccc gatgttcgcc acccacaacg cgcagaccat cgccaccgtg caccggatgg
     3961 cacgggcgat gcgcggccgc cgcgacttcg aattccagaa gctgcacggc atgggcgacg
     4021 acctgtacgc cgaggtgatc ccggccgacc ggctggacgt gccgtgccgg gtctacgcac
     4081 cggtcggcag ccacgaagac ctgctgccgt acctggtgcg ccggctgctg gagaacggcg
     4141 ccaactccag cttcgtcaac cgcatcaccg acgaatccat cccggtcgag gaactcgtgc
     4201 gcgacccggt ggagttcgtg tccgcgctgg agcacatcca gcatccccgc atcccgctgc
     4261 cggtcaacct gtaccgcagc caccaccagc atcgagacaa ttccatgggc atcaatctgg
     4321 ccaacgacga ccagctgcgc gaacttgccg cggcgctcaa cacgtccggc gcgggcagct
     4381 gggcggccgg cccgctggtg ccgggcgccg ccaatgccgg cgaccgcatc gcggtcagca
     4441 atcccgccga ccgtcgcgag gtggtgggcc actggcaggc cgccgacgcc gccacggtgg
     4501 aacaggcgct gcgcaacgcc agcgccgccc acgccggctg ggacgccacc ccggccggct
     4561 cgcgcgccgc catcctggag cacgccgcca gcctgctgga agcacgcatg ccggcctaca
     4621 tggcgatgtg caccaaggaa gccggcaaga ccctggccga cggcatcgcc gaagtgcgcg
     4681 aggcagtgga cttcctgcgc tactacgcgc tgcaggcacg cgagcacttc gccccggtcg
     4741 ccctgcccgg ccccaccggc gaggccaacc agctgcagct ggccgggcgc ggcgtgttcg
     4801 cctgcatcag cccgtggaat ttccccctgg ccattttcct cggccaggtc gcggccgcac
     4861 tggcggcggg caataccgtg atcgccaagc cggcggagca gaccaacctg atcggctttg
     4921 ccgcggtgcg gctgctgcac gaggccggca tcccgcagga cgtgctgcag ttcgtgccgg
     4981 gcgacggcgc caccgtgggt gcggcgctga cccgcgatcc gcgcgtggcc ggcgtctgct
     5041 tcaccggctc caccgaaacc gcgcgcctga tcaaccgcgc gctggccagc cgcgaggccg
     5101 gtccgatcgc caccctgatc gccgaaaccg gcggccagaa cgcgatgatc gccgactcct
     5161 cctcgctgcc ggaacaggtg gtcaaggatg cgctgggctc ggcctttacc tcggccggcc
     5221 agcgctgctc ggcggcacgc gtgctgctgg tgcaggagga catcgccgac aaggtgatcg
     5281 gcatgctggc cggcgcgatg gccgaactca ccgtcggcga ccccggcctg ccgtccaccg
     5341 acgtcggccc ggtgatcgac gaggacgccc gggcgatcct ggtcgaacat gccgcgcgca
     5401 tggatgccga ggccaggccg atcgcgaagg ccgcgctggg cgcgggctgt gagcacggca
     5461 ccttcttcgc gccggccgcg tgggagctgc gttcgatcgc ccaactggac cgcgagaagt
     5521 tcggcccggc gctgcacgtg gtgcgctgga aggcggagga actcgatgcg gtgatcgaca
     5581 ccatcaacgc caccggctac ggcctgaccc tgggcatcca ctcgcgcatc gacgccacca
     5641 tcgaccggat cgtctcgcgg gcgaaggtcg gcaacatcta cgtcaaccgc aaccagatcg
     5701 gcgcggtggt cggggtgcag ccgttcggcg gccagggcct gtccggcacc ggcccgaagg
     5761 ccggcggccc gcactacctg ccgcgcttcg ccaccgagaa gaccgtcacc gtcaacacca
     5821 ccgccgccgg cggcaacgcc agcctgctga ccctgggcga ctgaggcagg cctccccccc
     5881 gccaccgcgg gtggggggcg caacaacgaa aaagccccgc cgaagcgggg cttttcctgt
     5941 tgcgctggtg ccgtggatca gaagcggtac tgcatcgaca cgccgaacag gttggcgtag
     6001 ccaccgaagg taccgtgcag ggtcgacagg tcgctcggca cgtgcacgcc caggtcgatc
     6061 gtcggatcgt cgatctcgat gcgctggtag gcggcatcca cggtcaggtt gtcgctgaac
     6121 gcccagctca tgccgaccga gtacagcatg cggtcgttgt cgggcaggcg cggggtgcgg
     6181 gtcacgtcgt tggtcgggct ttcgtcccag ccgatgccgc cacgcagggt gaaggcgtcg
     6241 ctgaggtcgt actccatgcc gaccgagaag aagtcggtgt cttcccagct gaagtcctcg
     6301 ctgccgacca cggtgccg
//
LOCUS       DAORUK010000123         6279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_14621_length_6279_cov_17.570855, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000123 DAORUK010000000
VERSION     DAORUK010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14621_length_6279_cov_17.570855"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PK818_08280"
     CDS             complement(<1..472)
                     /locus_tag="PK818_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HQY82764.1"
                     /translation="MIFETLDTFGHEQVVFCHNKDAGLKAIIAIHNTTLGPALGGTRM
                     WPYKSEAEALNDVLRLSRGMTYKNAVAGLNIGGGKAVIIGDPATDKSEALFRAFGQFV
                     ESLGGRYITAEDVGIDVNDMEYVYRETEYVTGVHQVHGGSGDPSPFTAYGTLQGL"
     gene            complement(649..3672)
                     /locus_tag="PK818_08285"
     CDS             complement(649..3672)
                     /locus_tag="PK818_08285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQY82765.1"
                     /translation="MPAKHIALAALLCASALPCTAAAGAAVPKDLDLQRMGWLPSEVY
                     DVGEGLPDPTVNAIATLPNGQMWFGTMRGLARQSGARMQAEPGPDGILSGAILDLAAT
                     DSGDLLAATDARGIWRLRDGTWSALGAPFGKLRVQRLRLFGKGKLQRVFAIGGGVAEL
                     VDGRWRALALPPAVRAGEQFDIALEAARDGQPETLWVASYGPGLYRCAGPRDCSAVTI
                     PGPGPRTDEIRSLQLQPMAAGRASLWVGMQGGGVARLQDGAWTRWHIGNSTLPSDFVA
                     DLEVVPTPGGDTEVWAGTRSGLAILRGDGSWSGADPRVSLLRERVRTLASTRNSQGVP
                     VLWVGTDGGAVRSPLQGPWHLVSTLGKHGNGIWSLRVERAADGGKRLWLGSDGDGLAR
                     YEQGRWRIHRTADGLPNDTVRSLLRVPDGSAEGALWVGTWGGHVARLQGERFVEMPTP
                     WPKQDSEALSLLLADGDHVWGSTRQQGIVHWDGRQWHLLPPGPAAPARAYAALRHGDD
                     VWFSTSDKGLARYRNGQWRLFRADIGLPEDALYDMRLIADGTGAPLLWIGSKQHGLLR
                     IDIRDPDRPRLVTRPALPVLPVSYVYGVVRDGRGDLMVCTDYGVFSWRRSGDGYLSTA
                     YHREDGLPHDECNANAMQVDEHGRVWIGTVGGAAVYTPPEARPRQPSPLLLTRLLVDG
                     KPLAPPDGVLRLPRPDSALELEYDLLSGDKEDHSRYRVTLVNGGIEAGDWQAANSHHF
                     ARLPSGSRVVRIEARDASGIAATPIELRIEVPQVWWRTPGARALQVLAALLLVWGLLK
                     LRERQLRHREEQLLGMVKERTAQLQKRESELRGANDELRRLSYTDPLTGLGNRRRLFE
                     TLDLHWRDAARKRSSLALLLIDLDHFKRFNDAHGHLAGDARLQQVARNVQSMLPIGAS
                     AARYGGEELCVLLPGHDNAAAMRVAERMRNAIAVLPADAALPEIEGITVTASIGVAAC
                     IPGLEQRPDVLIARADRALYAAKAAGRNRVELALS"
     gene            complement(3815..4621)
                     /locus_tag="PK818_08290"
     CDS             complement(3815..4621)
                     /locus_tag="PK818_08290"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HQY82766.1"
                     /translation="MPMLRPVPALALLVALAACRHAPEPAPQVLLLGEVHDNAAGHAA
                     RADLLRERIEAGWRPAIAMEQFDTAQQAALDAAMASCADAECVVAKVAPAKSSWTWAH
                     YTPVIALALQYKLPLRAANLSRADASKIVKGGFADGLPADVIARYQLDALPGPLLAAQ
                     ETEVRDSHCGALPEPLVSPMAKAQIARDVVMAETLRAHAANGVVLIAGNGHVRTDIGV
                     PFWLRRDALASHATGFLEPGSDATGFDAVRRIPVQARPDPCAGFRPPTPG"
     gene            4740..>6279
                     /locus_tag="PK818_08295"
     CDS             4740..>6279
                     /locus_tag="PK818_08295"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQY82767.1"
                     /translation="MHPSFDKAVLALAISLSLAVPSLLPLTANAAEPQATAAGAEQSV
                     TTPGGRATFLLPAGWTTRVQGNAVIIMPPEADGSRTAIVDTPAATADEAVAEAWKVLG
                     LTPKLLVATDAAPRDGWEQRRFYDYDIPANAKRVVNSAAYRKDKHWTVIAMDVDQAIA
                     EKRGSQFAKINQRFQPAGYTRETFAGRTAHKLDAARLQQVSDFIEQMRKDFEVPGVAI
                     GIVQDGKVVLSRGYGMRDLGKPDPVDGDTRFMIASNTKALTTLMLAKLVDAGKLDWNA
                     RARDVYPAFKLGDTATSDQVLVKHLICACTGVPRQDYEWLFEGEKQTPQSVMATLGTM
                     QPTSGFGELFQYSNPMAAAAGFIGGQVAHPGMELGAGYDKAMQSLVFDPLGMRNTTFD
                     YDRAQTGNYAAPHGYDIAHKVQVMDMGLNDTIKASRPAGAAWSTVNDLLRYVQMEIDH
                     GVLPGGQRYIGEAALLQRREPQIATGVDRHYAMALGVDNSDGVTVIDHGGDMGGFHSN
                     MMWWP"
BASE COUNT          940 a         2211 c         2221 g          907 t
ORIGIN      
        1 tcaggccctg cagggtgccg tacgcggtga acggggaggg atcgccggag ccgccgtgga
       61 cctggtgcac gccggtcacg tattcggttt cccgatacac gtattccatg tcgttgacgt
      121 cgatgccgac gtcctcggcg gtgatgtagc ggccgcccag cgattccacg aactggccga
      181 acgcgcggaa cagcgcctcc gacttgtcgg tggccgggtc gccgatgatc accgccttgc
      241 cgccgccgat gttcaggccg gcgaccgcgt tcttgtaggt catgccgcgg ctcaggcgca
      301 gcacgtcgtt cagcgcttcg gcttcgctct tgtacggcca catgcgggtg ccgcccaggg
      361 ccgggccgag ggtggtgttg tggatggcga tgatcgcctt caggccggcg tccttgttat
      421 ggcagaacac cacctgttcg tggccgaagg tgtcaagggt ctcgaaaatc attgcgggaa
      481 ctccggtcgg gaccgcccgc gggtgggtgg gtgccgacgg caaagtgggg aatggaagcg
      541 tcgtggcccg catcggcacc ggcacaggcc gcgatgcagg gcgcgcattc taagccatcg
      601 accgcggccg ggcttgaatg cgccggggta tggcgccgtg cccgcgggtc acgacagcgc
      661 cagctccacc cggttgcggc ctgctgcctt ggctgcgtaa agggcacggt ccgcgcgtgc
      721 gatcagcacg tcggggcgct gttccaggcc ggggatgcat gccgccacgc cgatgctggc
      781 ggtgaccgtg atgccctcga tctccggcag ggcggcatcc gcgggcagca ccgcgatggc
      841 gttgcgcatg cgctcggcga cccgcatggc ggccgcgttg tcgtggccgg gcagcagcac
      901 gcacagttcc tcgccaccgt agcgcgcggc gctggccccg atcggcagca tcgactgcac
      961 gttccgcgcg acctgctgca ggcgggcgtc cccggcaagg tggccgtggg cgtcgttgaa
     1021 gcgcttgaaa tggtcgaggt cgatcagcag cagggccagc gaactgcgct tgcgcgcggc
     1081 atcgcgccag tgcaggtcga gcgtctcgaa caggcggcgg cggttgccga gcccggtcag
     1141 cgggtcggtg taggacagcc gccgcagttc gtcgttggcg ccgcgcagtt cggactcgcg
     1201 cttctgcagc tgcgcggtgc gttccttgac catcccgagc agttgttcct cgcgatgccg
     1261 gagctggcgt tcgcgcaact tcagcaggcc ccacaccagc agcagtgcgg ccagcacctg
     1321 cagggcgcgc gcgccgggcg tgcgccacca cacctggggc acctcgatcc gcagctcgat
     1381 cggcgttgcg gcgatgccgc tggcatcgcg ggcctcgatc cggaccacgc ggctgccgga
     1441 gggcaaccgc gcgaagtgat ggctgttggc ggcctgccag tcgccggcct cgatcccgcc
     1501 gttgaccagg gtgacgcgat agcggctgtg gtcttccttg tcgccgctga gcaggtcgta
     1561 ctccagctcc agcgcgctgt ccggacgcgg caggcgcagc acgccgtcag gtggcgcgag
     1621 cggcttgcca tcgaccagca ggcgggtgag caggagcggc gagggctggc gcggacgtgc
     1681 ctccggcggc gtgtaaaccg cggccccgcc gacggtaccg atccacaccc tgccgtgctc
     1741 gtccacctgc atcgcattgg cgttgcattc gtcgtgcggc aggccgtcct cgcggtggta
     1801 ggcggtggac aggtatccgt cgccgctgcg gcgccagctg aagacgccgt agtcggtgca
     1861 gaccatcagg tcgccgcggc cgtcgcggac cacgccgtag acatacgaca ccggcagcac
     1921 cggcagcgca gggcgggtga ccagccgcgg ccggtccgga tcgcggatgt cgatccgcag
     1981 cagtccatgc tgcttgctgc cgatccacag cagcggtgcg ccggttccat ccgcgatcag
     2041 gcgcatgtcg tacagggcat cctccggcag cccgatgtcg gcccggaaca gccgccactg
     2101 gccgttgcgg tagcgcgcaa ggcccttgtc ggaggtgctg aaccagacat cgtcgccatg
     2161 ccgcaacgcg gcgtatgcgc gggccggcgc agccggtccg ggcggcagca aatgccattg
     2221 gcgtccgtcc cagtggacga tgccctgctg gcgggtgctg ccccagacat ggtcgccgtc
     2281 ggccagcagc aggctcagcg cctcggagtc ctgcttgggc cagggcgtcg gcatttcgac
     2341 gaagcgttcg ccctgcaggc gggccacgtg cccaccccag gtgccgaccc acagcgcgcc
     2401 ctctgcgctg ccatcgggaa cccgcagcag gctgcggacg gtgtcattgg gcaggccatc
     2461 agcggtgcgg tggatgcgcc agcgcccctg ttcgtagcgc gccagcccat cgccgtcgct
     2521 acccagccac aggcgcttgc cgccgtcggc ggcgcgttcg acgcgcaggc tccagatccc
     2581 gttgccgtgc ttgccgaggg tactgaccag atgccacgga ccctgcagtg gcgaacgcac
     2641 cgcgccgcca tccgtcccca cccacagcac cggcacgccc tggctgttgc gggtgctggc
     2701 cagcgtgcgt acgcgttcgc gcaggagcga aacccgcgga tcggccccgc tccagctgcc
     2761 gtcgccgcgc aggatcgcca gcccgctgcg ggtgccggcc cagacctcgg tgtcgccgcc
     2821 gggggttggt accacctcca ggtcggcgac gaagtcgctg ggcagggtgc tgttgccgat
     2881 atgccagcgg gtccacgcgc catcctgcag ccgcgccacc ccgcccccct gcatgcccac
     2941 ccacaggctg gcccggcccg cggccatcgg ttgcaactgc aggctgcgga tctcgtcggt
     3001 gcgcggcccg ggtccgggga tcgtgaccgc gctgcagtcc cggggtcccg cgcagcgata
     3061 caggccggga ccgtagcttg cgacccacag cgtctccggc tgcccgtcgc gggcggcttc
     3121 gagcgcgatg tcgaactgct cgcccgcgcg caccgcgggt ggcagggcga gcgcgcgcca
     3181 gcggccgtcg accagctcgg ccaccccgcc gccgatcgcg aacacccgct gcagcttgcc
     3241 cttcccgaac agccgcaggc gctgcacgcg gagcttgccg aacggtgcac ccagcgcgga
     3301 ccaggtgcca tcgcgcaggc gccagatgcc acgggcgtcg gtcgcggcca gcaggtcgcc
     3361 actgtcggtt gcggccaggt cgaggatcgc gccggacagt atgccgtccg gccccggctc
     3421 ggcctgcatg cgcgcaccgc tctggcgcgc cagcccgcgc atggtgccga accacatctg
     3481 cccgttcggc agggtggcga tcgcgttgac ggtggggtcg ggcaggccct cgccgacgtc
     3541 gtagacctcg gagggcagcc agcccatgcg ctgcaggtcg aggtccttcg gcacggccgc
     3601 gcccgccgcc gccgtgcagg gcagggcgga cgcgcatagc agtgcagcaa gggcgatgtg
     3661 cttggcgggc atggctcggg ccgggcttct cccgaaggaa gctagcaatc tccgtgccac
     3721 cgtaatgcga acgtcgtcac agaggccgcg tgccgcaatc gacatccacc ggtcggggcg
     3781 tcccccggcc ggcgcgggca gcggcgggca gcgctcaacc gggcgtgggc ggcctgaacc
     3841 cggcgcacgg atccggacgc gcctgcaccg ggatgcgacg caccgcgtcg aatccggtgg
     3901 cgtcagagcc gggttcgagg aaaccggtcg cgtgcgacgc cagcgcgtcg cggcgcagcc
     3961 agaacggcac cccgatatcg gtgcgcacgt ggccgttgcc ggcgatcagg accacgccat
     4021 tggcggcatg cgcgcgcagc gtctccgcca tcaccacgtc gcgcgcgatc tgcgccttcg
     4081 ccatcggcga caccagcggt tccggaagcg ccccgcaatg gctgtcgcgc acttcggttt
     4141 cctgcgcggc cagcagcggg ccgggcaggg cgtccaactg gtagcgcgcg atgacgtccg
     4201 caggcaagcc atcggcgaaa ccgcccttga cgatctttga agcatccgca cgcgacaggt
     4261 tggccgcgcg cagcggcaac ttgtactgca gggccagcgc gatgaccggc gtgtagtggg
     4321 cccaggtcca actggacttc gccggcgcca ccttcgcgac cacgcactcg gcatccgcac
     4381 agctcgccat cgccgcgtcc agcgcggcct gctgggcggt gtcgaactgc tccatcgcga
     4441 tcgccggccg ccagccggct tcgatccgtt cgcgcagcag gtccgcgcgg gcagcgtggc
     4501 cggcggcgtt gtcgtgcacc tcgcccagca acagcacctg cggcgcgggc tccggcgcgt
     4561 gccggcacgc ggccagggcg acaagcaggg caagggcggg gacgggacgc agcatgggca
     4621 tgaccggatc gggaaacgtc gcccagcttg ccacgggtcg gcatgaccga agtggggacg
     4681 gcatcccgtt atcacccccg tacgttccgc gcccgcgcat ccgccttctg gagagcacca
     4741 tgcacccttc gttcgacaag gccgtcctgg ccctcgcgat atcgctttcg ctggcggttc
     4801 cctcgttgct gccgctgacc gcaaacgccg ccgagccgca ggcgaccgcg gccggcgcgg
     4861 agcagtcggt gaccacgcca ggcggcaggg cgaccttcct gctgccggcg ggatggacca
     4921 ccagggtgca aggcaacgcg gtgatcatca tgccgcccga agcggacggc tcgcgcaccg
     4981 cgatcgtcga tacccccgcc gcgaccgcgg acgaggcggt cgccgaagcg tggaaggtgc
     5041 tcgggctcac gccgaaactg ctggtcgcca ccgatgccgc gccgcgcgac ggctgggagc
     5101 agcgccgctt ctacgattac gacatcccgg cgaacgccaa gcgggtggtc aactctgcgg
     5161 cctatcgcaa ggacaagcac tggacggtga tcgcgatgga cgtggaccag gccattgccg
     5221 agaagcgcgg ctcgcagttc gccaagatca accagcgctt ccagccggcc ggctacaccc
     5281 gcgagacctt cgccgggcgc accgcgcaca agctggatgc cgcgcgcctg cagcaggtca
     5341 gcgatttcat cgagcagatg cgcaaggact tcgaagtgcc gggcgtggcc atcggcatcg
     5401 tgcaggacgg caaggtggtg ctgtcgcgcg gctacggcat gcgcgatctc ggcaagccgg
     5461 acccggtgga tggcgacacc cgtttcatga tcgcttccaa caccaaggcg ctgaccaccc
     5521 tgatgctggc caagctggtc gatgccggca agctggactg gaacgcgcgc gcccgggacg
     5581 tgtatccggc cttcaagctg ggcgacaccg ccaccagtga ccaggtgctg gtgaagcacc
     5641 tgatctgcgc ctgcaccggc gtgccgcgcc aggactacga atggctgttc gagggcgaaa
     5701 agcagacccc gcagagcgtg atggccacgc tgggcaccat gcagccgacc agcggcttcg
     5761 gtgaactgtt ccagtattcg aacccgatgg cggcggcggc cggtttcatc ggcggccagg
     5821 tcgcgcatcc gggcatggag ctgggtgccg gttacgacaa ggcgatgcag tcgctggtat
     5881 tcgatccgct gggcatgcga aataccacct tcgactacga ccgggcgcag accggcaatt
     5941 acgccgcgcc gcacggctac gacatcgcgc acaaggtcca ggtcatggac atgggcctga
     6001 acgacaccat caaggcctcg cgcccggcgg gcgccgcgtg gagcacggtc aacgacctgc
     6061 tcaggtacgt gcagatggag atcgaccacg gggtgttgcc cggcggccag cgctacatcg
     6121 gcgaagccgc gctgctgcag cgccgcgagc cgcagatcgc gaccggcgtg gaccgccatt
     6181 acgcgatggc gctgggggtg gacaacagcg acggcgtcac cgtgatcgac cacggcggcg
     6241 acatgggcgg cttccactcc aacatgatgt ggtggccgg
//
LOCUS       DAORUK010000124         6149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_15133_length_6149_cov_20.241057, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000124 DAORUK010000000
VERSION     DAORUK010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15133_length_6149_cov_20.241057"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PK818_08300"
     CDS             complement(<1..229)
                     /locus_tag="PK818_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010380232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HQY82768.1"
                     /translation="MGKIIGIDLGTTNSCIAIMEGGKAKVIENSEGDRTTPSIVAWTK
                     DGEVLVGASAKRQAVTNPKNTFYAVKRLIGRK"
     gene            complement(330..833)
                     /gene="grpE"
                     /locus_tag="PK818_08305"
     CDS             complement(330..833)
                     /gene="grpE"
                     /locus_tag="PK818_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HQY82769.1"
                     /translation="MTHDPMQEANEAAFDTPPTDETEALRAELEQLKAQSLLERADLE
                     NQRKRMAREVEMARKFANERLLGDLLPVLDSLEAGLAAAPADDPLRAGLELTQRQLVR
                     VAEINGLVAVMPAAGDAFDPERHQAMSMIDADGIAPGAVAQTFQKGYLLNDRLLRPAM
                     VVVARQD"
     gene            complement(901..1950)
                     /gene="hrcA"
                     /locus_tag="PK818_08310"
     CDS             complement(901..1950)
                     /gene="hrcA"
                     /locus_tag="PK818_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HQY82770.1"
                     /translation="MARPGAPDIGNDPRARQLLRTLVSRHIRDGEPVGSQTLARHAGL
                     DVSAATIRNILASMEDAGLLAAAHSSAGRVPTAQGYRLFVDSLLQVKPLPEGELIRLR
                     HELAAGAGTQALLGNTSELLSAMTHFVGMVSVPSRASFAFRQIEFVALDPQRVLAILV
                     FADGEVQNRVVQVRRAFDPSELEQAANYFNSHFAGQPLAHIRAALLHDLRHARSEMER
                     LLASSVELAEQAFAPGREDMLVAGQTRLMGLQELSDLDRLRALFEAFAEKRELLQLLE
                     RTARAPGMRVFIGEETGLAPLEGMSLVTAPYGSGGRMLGVLGVIGPSRMAYERVIPVV
                     EATAAVLGAALSPDA"
     gene            2028..3692
                     /gene="recN"
                     /locus_tag="PK818_08315"
     CDS             2028..3692
                     /gene="recN"
                     /locus_tag="PK818_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017116147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HQY82771.1"
                     /translation="MLSRLSIRDFAVVAHAELEFGAGLTVVSGETGAGKSLLVDALGF
                     LCGARADAGAVRHGAGRAELEADFSLADCPAALAWLRESELDDGGACQLRRTLRADGG
                     SRAWINGRGASLAQLAELAGLLVEIHGQHEQQALLSRATQTGLLDGFARHPELLAAVR
                     ETALRWRGLRDARDALLGRGDVGERKAWLQHQLDELQAETLDPGALAELDASHRRHAH
                     AASLIAACEHALEQLGGEQALAAQLQQVRSGLLREAGNEPRLHEVEAMLDSAAIELDE
                     ALGLLARIRDDLDLDPAVLQQIEARLVRVQDLARKHRIAPAGLSAHRDALAAELESLA
                     DADARLLALDRDIALALADWNAAASALTASRRKAGDALAQSVQALIAELGMGGGRFEV
                     ELETVADSRSPQANGAERAEFLVSANAGQLPRPLRKVASGGELSRISLAIEVATLGVD
                     AVPTMVFDEVDSGIGGAVAAAVGARLRALGETRQVLCVTHQPQVAAAGHAHYRVSKAA
                     RDGMTQSSVQVLDADGRVGEIARMLGGARVTTEAQAAARTLLVESR"
     gene            complement(3724..4125)
                     /gene="fur"
                     /locus_tag="PK818_08320"
     CDS             complement(3724..4125)
                     /gene="fur"
                     /locus_tag="PK818_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002804358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HQY82772.1"
                     /translation="MESQDLRKAGLKVTHPRMRILELLEQTRPRHMTAEDIYRHLLGG
                     GEEIGLATIYRVLTQFESAGLVLKHNFEGGHAVYELDRGEHHDHMVDVDTGKVIEFHS
                     DEIEALQHRIAAEHGYLIEDHALVLYVRKRT"
     gene            4253..4639
                     /gene="bamE"
                     /locus_tag="PK818_08325"
     CDS             4253..4639
                     /gene="bamE"
                     /locus_tag="PK818_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006449455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HQY82773.1"
                     /translation="MRKLLALVLVIMFASGCGVLYRQPIYQGNLLDKAAVEQLQQGMD
                     KRQVMTLLGTPSIRDPFHQDRWDYTASRRTGRSGPTDVKNMTLFFENEALAKWEGEYY
                     PEEDSELAKTMAQFGNLAKDKKKGGR"
     gene            complement(4653..4922)
                     /locus_tag="PK818_08330"
     CDS             complement(4653..4922)
                     /locus_tag="PK818_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HQY82774.1"
                     /translation="MIRVELVRAWPHRHDSVAVDVADGASVADALEAAGWRLDAEFMG
                     LAVFGQAATMETPLHDDDRIELLRALQIDPKQARRLRAARAKGAP"
     gene            complement(4919..5356)
                     /locus_tag="PK818_08335"
     CDS             complement(4919..5356)
                     /locus_tag="PK818_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016901567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HQY82775.1"
                     /translation="MPEIVRSALVEHSAARMFALVNDIAAYPRRFDWCRAASVLETGE
                     DRMLARLELGVGGFHTWFTTENALVPPHHIDMALRDGPFKRLHGRWQFHALDESACKV
                     TLTLDFEPQTRLLGPALALGMQGLADRMVDDFVREADRGGQAA"
     gene            5425..5919
                     /gene="smpB"
                     /locus_tag="PK818_08340"
     CDS             5425..5919
                     /gene="smpB"
                     /locus_tag="PK818_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011050971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HQY82776.1"
                     /translation="MNKKAGKDKPNGGGTIALNKRARHEYHLEQRFEAGMALQGWELK
                     AIRAGRANIGDAYAVVLEGELFLIGSQITPLISASTHVVANDRRTRKLLMHRREIDVL
                     IGRVQREGYTLVPTALYWKGNKVKMELALAHGKQSHDKRDATRERDWNREKQRVMRRH
                     NKNA"
BASE COUNT          910 a         2088 c         2207 g          944 t
ORIGIN      
        1 acttgcggcc gatcaggcgc ttcaccgcgt agaaggtgtt cttcgggttg gtgaccgcct
       61 ggcgcttggc cgaggcgccg accagcactt cgccgtcctt ggtccaggcg acgatggacg
      121 gcgtggtgcg gtcgccctcg ctgttctcga tgacctttgc cttgccgcct tccatgatgg
      181 cgatgcagga gttggtggtg ccgaggtcga tgccgatgat cttgcccatg tgtgtgtcct
      241 cttcgatctg gatggcgggg gcgccgtgcg ccgatgtccg cgatgtgggg ttgccgcgtg
      301 cggcttcaag tgccgcggct gcgggtcgtt cagtcctggc gcgcgaccac gaccatggcc
      361 gggcgcagca ggcggtcgtt gagcaggtag cccttctgga aggtctgcgc gaccgcgccc
      421 ggcgcaatgc cgtcggcgtc gatcatgctc atcgcctggt ggcgttccgg gtcgaaggcg
      481 tcgcccgcgg cgggcatcac cgcgaccagg ccgttgatct cggcgacccg caccagctgg
      541 cgctgggtca gctcgaggcc ggcgcgcagc gggtcgtcgg cgggtgccgc ggccaggccg
      601 gcttccaggc tgtcgagcac cggcagcagg tcgccgagca ggcgctcgtt ggcgaacttg
      661 cgcgccatct ccacttcgcg ggccatgcgc ttgcgctggt tctcgaggtc ggcgcgctcc
      721 agcagggatt gcgccttcag ttgttcgagt tcggcgcgca gcgcctcggt ttcgtcggtc
      781 ggcggcgtat cgaaggccgc ttcgttggct tcctgcatgg gatcgtgggt catggtccaa
      841 gcgctgattg cggcaccgtg ccgcgtccac cgccagtgat ggggccggcc cggcggggat
      901 tcaagcgtcc ggagacagcg ccgcgcccag caccgcggcg gtggcctcca ccaccgggat
      961 cacccgctcg tacgccatcc ggctggggcc gatcaccccg agcacgccca gcatgcggcc
     1021 accgctgccg tagggcgcgg tcaccagcga catcccctcc agcggtgcca acccggtttc
     1081 ctcgccgatg aacacccgca tgcctggcgc cctggcggtg cgttccagca actgcagcag
     1141 ttcgcgcttc tcggcgaagg cctcgaacaa cgcgcgcagg cgatcgaggt cgctgagctc
     1201 ctgcagcccc atcagccggg tctggccagc caccagcatg tcctcgcggc cgggggcgaa
     1261 cgcctgctcg gcgagttcga ccgaactcgc cagcagccgt tccatctcgc tgcgggcgtg
     1321 gcgcaagtcg tgcagcagtg cagcgcggat gtgcgccagc ggttgcccgg cgaagtggct
     1381 gttgaagtag ttggcggcct gttccagttc ggacgggtcg aacgcgcgcc ggacctggac
     1441 cacccggttc tgcacttcgc catcggcgaa caccaggatc gccagcaccc gctgcgggtc
     1501 cagcgcgacg aactcgatct ggcggaaggc gaagctcgca cgcgaaggca cgctgaccat
     1561 gccgacgaaa tgggtcatcg ccgacagcaa ctccgaggtg tttccgagca gggcctgggt
     1621 gccggcgccg gccgccagtt cgtggcgcag gcggatcagc tcgccctccg gcagcggctt
     1681 gacctgcagc agcgagtcca cgaacagccg gtagccctgc gcggtcggca cccgcccggc
     1741 cgagctgtgg gcggcggcca gcaggccggc gtcctccatc gaagcgagga tgttgcggat
     1801 cgtcgccgcg ctgacgtcga ggccggcatg ccgggccagg gtctgcgatc ccaccggctc
     1861 gccgtcgcgg atgtgccgcg acaccagcgt gcgcagcaat tgccgtgcgc gcgggtcgtt
     1921 gccgatgtcg ggggcgccgg ggcgtgccat gccgatgttg taagcgccgc tcccgccgcg
     1981 cgcaagcgct atcgaagcgg cgcccggagc aggcacaatg cgcgccgatg ctgagccgcc
     2041 tgtccatccg cgatttcgcc gtggtcgccc atgccgagct ggaattcggc gcgggcctga
     2101 ccgtggtgtc cggcgaaacc ggtgccggca agtcgctgct ggtggacgcg cttggcttcc
     2161 tgtgcggcgc ccgcgcggat gccggcgcgg tgcgccacgg cgccgggcgc gccgagctgg
     2221 aggcggactt ctcccttgcc gattgcccgg cggcgctggc ctggctgcgc gaatcggaac
     2281 tggacgacgg cggcgcctgc cagttgcgac gcaccctgcg cgcggatggt ggttcgcgcg
     2341 cctggatcaa tggccgcggc gcgagcctgg cccagctggc ggaactggcg ggcctgctgg
     2401 tggagatcca cggccagcac gagcaacagg ccctgctctc gcgcgcgacc cagaccggcc
     2461 tgctggacgg ctttgcgcgg catccggagt tgctggccgc ggtgcgcgaa accgcactgc
     2521 gctggcgcgg cttgcgcgat gcgcgcgacg ccctgctcgg gcgcggcgat gtcggcgagc
     2581 gcaaggcgtg gctgcagcac cagctggatg agctgcaagc ggaaacgctg gacccggggg
     2641 cattggcgga gctggatgcc agccatcgcc ggcatgcgca cgcggcgtcg ctgatcgcag
     2701 cctgcgagca tgcgctggag caactcggcg gcgagcaggc gcttgccgcg cagttgcagc
     2761 aggtgcgttc cgggctgctg cgcgaagcgg gcaacgagcc acgcctgcac gaggtggagg
     2821 cgatgctgga ttcggccgcg atcgagctcg acgaggcgct tggcctgctc gcgcgcatcc
     2881 gcgacgacct cgacctggat cccgcggtgc tgcagcagat cgaagcgagg ctggtgcgcg
     2941 tgcaggacct ggcccgcaag caccgtatcg cgccggccgg actgtccgcg catcgcgacg
     3001 cgctggccgc cgagctggaa tcgctggccg acgcggatgc gcggttgctg gccctggatc
     3061 gcgacatcgc gctggcactc gcagactgga acgccgcggc atccgcactc accgcctccc
     3121 gccgcaaggc cggtgatgcg ctggcgcagt cggtgcaggc actgatcgcc gagctcggga
     3181 tgggcggcgg ccgcttcgaa gtggaactgg aaacggtcgc ggattcgcgc tcgccgcagg
     3241 ccaatggcgc cgaacgcgcg gaattcctgg tctccgccaa cgccggccag ctgccgaggc
     3301 cgttgcgcaa ggtcgcctcc ggcggcgagc tgtcgcgcat ttcgctggcg atcgaagtcg
     3361 ccaccctcgg ggtggacgcg gtgccgacca tggtcttcga cgaagtggat tccgggatcg
     3421 gcggcgcggt cgcggccgcg gtcggtgcca ggctgcgtgc gctcggcgaa acccggcagg
     3481 tgttgtgcgt gacccaccag ccgcaggtcg cggccgccgg ccatgcgcat taccgggtca
     3541 gcaaggccgc ccgcgacggc atgacccagt cgtcggtgca ggtgctggac gcggacggac
     3601 gggtcggcga gatcgcgcgc atgcttggcg gtgcgcgggt aaccaccgag gcgcaggccg
     3661 cggcgcggac gctgctggtg gaaagccgct agtcgcggcc tcggtgatgc gcggcgggcc
     3721 ggctcaggtc cgcttgcgca cgtacagcac cagcgcgtgg tcctcgatca ggtagccatg
     3781 ctcggcggcg atccggtgct gcagggcttc gatctcgtcg ctgtggaatt cgatcacctt
     3841 gccggtatcg acgtcgacca tgtggtcgtg gtgctcgccg cggtcgagtt cgtagaccgc
     3901 gtggccgccc tcgaaattgt gcttgagcac cagcccggcg gattcgaact gggtgagcac
     3961 gcgatagatc gtggccaggc cgatttcctc gccgccaccg agcaggtgcc ggtagatgtc
     4021 ttccgcggtc atgtgccgcg gccgggtctg ttccagcagc tccaggatcc gcatccgcgg
     4081 atgggtcacc ttcagtccgg ccttgcgtag gtcctgcgat tccatcggtt ctccgtcgcg
     4141 cgaacggcgc atgaatgccg gctgcggagg cggcccggga cgcggccgca ggcgccgtgc
     4201 tgcgaggcct tcagtgtatc atcgggcctt cgttcactgc cccctgccgc cgatgcgcaa
     4261 gctgctcgct cttgtcctcg tgatcatgtt cgccagcggt tgcggcgtgc tgtaccggca
     4321 gccgatctac cagggcaacc tgctcgacaa ggccgcggtg gagcaactgc agcagggcat
     4381 ggacaagcgc caggtgatga ccctgctggg cacgccctcg atccgcgacc cgttccacca
     4441 ggatcgctgg gactacaccg ccagccgccg caccggccgt tcgggcccga ccgacgtgaa
     4501 gaacatgacc ctgttcttcg agaacgaggc gttggcgaag tgggaaggcg agtactaccc
     4561 cgaggaagat tccgaactcg ccaagaccat ggcccagttc ggcaacctcg ccaaggacaa
     4621 gaagaagggc ggccgctgac gcaaggccct gctcagggcg cgcccttcgc gcgcgccgcg
     4681 cgcagccgcc gcgcctgctt cggatcaatt tgcagcgcac gcagcagttc gatgcggtcg
     4741 tcgtcgtgca gaggtgtttc catggtcgcg gcctgaccga acaccgccag gcccatgaac
     4801 tccgcgtcca gccgccagcc ggcggcctcc agcgcatcgg cgacgctggc gccatccgcg
     4861 acatcaaccg cgaccgagtc atgccggtgc ggccacgccc gcaccagttc gacccggatc
     4921 acgccgcctg cccgccgcgg tccgcctcgc gcacgaaatc gtcgaccatg cggtcggcca
     4981 ggccctgcat gcccagcgcc agcgccggcc caagcagccg ggtctgcggc tcgaagtcga
     5041 gggtcagggt caccttgcac gcggactcgt ccagtgcatg gaattgccag cgtccgtgca
     5101 ggcgcttgaa cgggccgtcg cgcagcgcca tgtcgatgtg gtgcggcggc accagcgcgt
     5161 tttcggtggt gaaccaggtg tggaagccgc cgacgccgag ctccagccgg gcgagcatgc
     5221 ggtcctcgcc cgtttccagc acgctggccg cgcggcacca gtcgaagcgc cgcggatagg
     5281 cggcgatgtc gttgaccagc gcgaacatgc gcgcggcgga atgctcgacc agggcgctgc
     5341 ggacgatttc gggcatcgcg gttccatggg gctgcgccgg tcgccggcga gacgccttcg
     5401 cccggatcgg cgacaatgcc gacgatgaac aagaaggccg gcaaggataa gccaaacggc
     5461 ggcggcacca tcgccctgaa caagcgggcg cgccacgaat accacctgga gcagcggttc
     5521 gaggccggca tggccctgca gggctgggag ctgaaggcga tccgcgccgg ccgcgccaac
     5581 atcggcgatg cctacgcggt ggttctggaa ggcgagctgt tcctgatcgg ctcgcagatc
     5641 accccgctga tctcggcgtc gacccacgtt gttgccaacg atcgcaggac ccgcaagctg
     5701 ctgatgcacc ggcgcgagat cgacgtgctc atcggtcgcg tgcagcgcga gggctacacg
     5761 ctggtaccga ccgcgctgta ctggaagggc aacaaggtga agatggaact ggcgctggcg
     5821 cacggcaagc agagccacga caagcgcgac gccaccaggg aacgcgactg gaaccgcgag
     5881 aagcagcggg tgatgcggcg ccacaacaag aatgcgtgag gggccaggca tcgccgggac
     5941 cgcgctgggc gggatcggca tcgcccggca aggacaagga catgagcgca ggataaccgc
     6001 ggcgccggca acgccgcgtc aaccttgtgt cgcgcgcacc cgcccctata ctgcgactgt
     6061 cggtgagatc ggcaattccc gggggtgcac tggcttcgac gggggtagcg aaatcgcacg
     6121 gcgcatgccg agggggtagc tttcctcgt
//
LOCUS       DAORUK010000125         6144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_15148_length_6144_cov_18.525702, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000125 DAORUK010000000
VERSION     DAORUK010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15148_length_6144_cov_18.525702"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PK818_08345"
     CDS             <1..645
                     /locus_tag="PK818_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82777.1"
                     /translation="PPPPPPPPTQVGGEQAIPAFPWPPPKPTSRTTMDRSLLARNDGA
                     TLGEVARHLEGALSGVGYGERSYYTVPGGFALATRLEQITPDGTPKPDPQRWSTALPE
                     QPVFSLGDYLRALFAAPQGDYRVIVFIVTNRAFATSGRPATNEQAEAWLSGGLDRLPR
                     SIQALPFDADVEASALVYQFRKSGHGDPAVALASSTTSAATQLDRSGIVAALRR"
     gene            653..1123
                     /locus_tag="PK818_08350"
     CDS             653..1123
                     /locus_tag="PK818_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82778.1"
                     /translation="MTLTRSRWMFAGIWLGGAGLVFLILVVQSLVGRYGSQSEDAWAW
                     YLPTVMPTLSLIIGVLATDFRVAASAAAAATTTTGTSAASPDANILPVAARGLLWLGV
                     ALSVFYLLLVAVTILAQPFLQGVSPIELMHRSNLWLGPLQGLTAGVLAAFFRSG"
     gene            complement(1151..4660)
                     /locus_tag="PK818_08355"
     CDS             complement(1151..4660)
                     /locus_tag="PK818_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQY82779.1"
                     /translation="MPRRLGTLLGLWLLCACALAAVPERPRLRIVGAGQGLPSTAIKA
                     LARDRDGYLWVATADGLARHDGVGMRLWRHQPGDPRGLPGNNVQALLVDARDRVWVAT
                     EGGGISVLDAQRQGFVHYRKATHPQLGSDDIWAFAQHAGSVWFGAYDGGLHRIGDDGT
                     IHRYAAASDGLPSDTVLALAVDAGGALWIGTDAGLARMQGGRIEGVRLPGTDAPPLVY
                     SLTMQADGLWVGTSDGVWRRDAGGDWTQPAWSPMFRRPNAMNAIVRARDGGSWIASQR
                     GLWRQQGAEPPSPVRLGGPDIPRGITAMLQDADGALWVPVAGVGLGYLRPDWRQLAQY
                     AGETDGLQGAMYRALAPARDGGFWLGGFNGMVERLATDGGIGRLDDDSLARLRSIKLL
                     AIAEDTGGRLWLGHRNGLLRVGSDGAIDEWRSGDPRDATPPGQVDQLQVAPDGSLWLS
                     APGAGVQQRDPASGRVLREIPADAAHGLATGDIEALVVSPRGEVWVAGADGVAVLDAA
                     QGTFRRLPGFGGERVYALAFDGDRMLWLQRQSGLVHYVARGGRWQAADRVDSAHGMPA
                     VGASGMRVDLRHRPWVSTSRGLYRYDPRTRNLRRHGIRDGSGSQEFVDHALAMSAEGV
                     LAAATADGGIVLVDTTAADPVATVPSLRYDRLSVRRNGEWRDIPLQAGLRLGRDDREF
                     RIRARLLAFEDPESNRYWSRLDGFDRAWVALGASGDRVFTGLAPGRYTLRMRARDAAG
                     NAAREQRLAFAVPPPWWRSWWALLLYLVLALLAVAAAAAAYRARLKRRHALQLTEAKR
                     VLAEQASEAKSRFLATLGHEVRTPMTGVLGMSELLRATRLDDRQRSQVDAIHRAGEHL
                     LRLVNDALDLARIEAGKLELATADFALRPLLDEVAALMAPVAERKGLAFVDSMDAGTP
                     AALHGDRTRIQQILLNLLGNAIKFTESGHVSLETSALAPRGVRFVVADSGPGLSREQQ
                     ARLFRRFEQADGARTAARYGGSGLGLAISQELATAMGGGIAVESAPGQGSRFIVELPL
                     VHADMQPPPASAAPLRAVAGSLRLLLVEDDPIVAEVMQGLLREQGHVVVHAGHGLGAL
                     AEVASQRFDAGLLDLDLPGLDGLALARMLRAQGFVAPLLAVTARSDADAEAQAHAAGF
                     DDFLRKPVSGAMLAQALHARVPQ"
     gene            complement(4702..4929)
                     /locus_tag="PK818_08360"
     CDS             complement(4702..4929)
                     /locus_tag="PK818_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HQY82780.1"
                     /translation="MGLILYQRDHCHLCDLALEALAQARTPEFESVFIDGDAALEARF
                     GVRVPVLRDAASGRELDWPFDPEGVRRFLLA"
     gene            4997..>6144
                     /locus_tag="PK818_08365"
     CDS             4997..>6144
                     /locus_tag="PK818_08365"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HQY82781.1"
                     /translation="MAVSTFDLYKIGIGPSSSHTVGPMRAAARFVEHHLAEAGRLPQV
                     ARVRAEVFGSLALTGRGHGTDKAVMMGLEGHWPNRIDPDIIPAALQRIRGEKRITLHG
                     THEVGFDEKRDLVMNKRQKLPFHTNGMRFIAYDAAGNELASRDYYSVGGGFVVNQDEA
                     AEDRIVADTTVVAHPYASGDELLARCAQSGLSIAQLMLANERAWRSDDAIHAGLRELW
                     QAMQDCVARGIRASGTLPGGLHVVRRAPSLHAELAAKPEAAMRDPLTVLDWVNLYALA
                     VNEENAAGGRVVTAPTNGAAGIIPAVLHYYDRFIPGANEQGVFDFLLTAAAVGILYKE
                     NASISGAEVGCQGEVGVACSMAAAGLAAALGGSPGQIENAAEIGMEHNL"
BASE COUNT          883 a         2356 c         2092 g          813 t
ORIGIN      
        1 ccaccgccgc ctcccccacc gcccacgcag gtcggtggcg aacaggcgat ccccgcgttt
       61 ccatggccgc cgccgaagcc cacctcgcgc accacgatgg atcgcagcct gctggcgcgc
      121 aacgacgggg ccacgctggg cgaggtcgcc cgccacctgg aaggcgcact gtcgggcgtc
      181 ggctatgggg aacgcagcta ctacacggtg cccggcggct tcgcgctggc gacgaggctc
      241 gaacagatca cgcccgacgg cacgccgaag ccggacccgc agcgctggtc caccgcgctg
      301 cccgaacagc cggtgttctc gctgggcgac tacctgcgcg ccctgttcgc cgcgccgcag
      361 ggcgactacc gggtcatcgt cttcatcgtc accaaccggg cgttcgccac ctccggacgc
      421 ccggcgacga acgagcaggc cgaggcctgg ctgagtggcg ggctggaccg cctgccgcgc
      481 tcgatccagg cactgccgtt cgatgccgat gtcgaggcca gcgcgctggt ctaccagttc
      541 cgcaagagcg gccatggcga tccggcggtt gcgctggcaa gcagcaccac atccgccgca
      601 acccaactcg atcgcagcgg catcgtcgct gcgctgcgcc gctgaggccc gcatgaccct
      661 gaccagatcc agatggatgt tcgccggcat ctggctcggc ggcgcgggcc tggtgttcct
      721 gatcctggtg gtgcagagcc tggtcggccg ctacggctcg caaagcgagg acgcctgggc
      781 gtggtacctg cccaccgtga tgccgaccct gtcgctgatc atcggcgtgc tggccaccga
      841 tttccgcgtc gccgcgagcg cagccgcggc cgccacgacg acgaccggta cctctgcagc
      901 cagccccgac gcgaacatcc tgccggttgc cgccaggggc ctgctctggc tgggcgtggc
      961 gctgtcggtg ttctacctgt tgctggtcgc ggtcaccatc ctcgcgcagc cgttcctgca
     1021 gggcgtgtcg ccgatcgagc tgatgcaccg ctccaacctg tggctgggcc cgttgcaggg
     1081 gctgactgcc ggcgtgctcg ccgcgttttt ccgctcgggt tgatcgcgac gccttgccgg
     1141 cgccgcgttg ctactgcggc acgcgggcgt gcagcgcctg cgccagcatc gccccgctga
     1201 ccggcttgcg caggaaatcg tcgaagccgg ccgcgtgggc ctgcgcctcg gcatccgcgt
     1261 ccgaacgcgc ggtcaccgcc agcagcggcg ccacgaagcc ctgtgcgcgc agcatgcgcg
     1321 cgagcgcaag gccatccagt cccggcaggt cgaggtcgag caaaccggcg tcgaagcgct
     1381 ggctggcgac ttccgccagc gcacccagcc catgcccggc atgcaccacc acatgcccct
     1441 gttcgcgcaa caagccctgc atgacctcgg ccacgatcgg atcgtcctcg accagcagca
     1501 gccgcagcga tcccgcgacc gcccgcaacg gtgccgcgga tgcaggcggc ggctgcatgt
     1561 cggcatgcac cagcggcaac tcgacgatga agcggctgcc ctgccccggc gcactttcga
     1621 ccgcgatccc gccgcccatc gccgtcgcca gctcctggct gatcgcaagc cccagcccgc
     1681 tgccgccgta gcgcgcggcg gtgcgtgcgc catcggcctg ctcgaagcgg cggaacagcc
     1741 gggcctgctg ctcgcggctc aagcccgggc cactgtcggc gaccacgaag cgcacacccc
     1801 ggggtgcaag cgcactggtt tccagcgaca cgtggccgga ctcggtgaac ttgatcgcat
     1861 tgccgagcag gttgagcagg atctgctgga tgcgggtgcg gtcgccgtgg agcgccgctg
     1921 gcgtgccggc atccatgctg tcgacgaatg ccaggccctt gcgttccgcc accggcgcca
     1981 tcagcgcggc gacctcgtcc agcagcgggc gcagcgcgaa gtcggcggtc gccagctcca
     2041 gcttgccggc ttcgatccgg gccaggtcca gcgcatcgtt gaccagccgc agcaggtgct
     2101 cgccggcgcg atggatggca tcgacctggc tgcgctgcct gtcgtcgagc cgggtcgcgc
     2161 gcagcagttc gctcatgccg agcacgccgg tcatcggcgt gcgcacctcg tggccgagtg
     2221 tcgccaggaa gcgcgacttc gcctccgaag cctgttcggc cagcacccgt ttcgcctcgg
     2281 tcagctgcag tgcgtgccgg cgcttgagcc gggcccgata ggccgcggcc gcggcggcca
     2341 ccgccagcag cgcgagcacc aggtacagca gcagcgccca ccagctgcgc caccacggcg
     2401 gcggcaccgc gaacgccagc cgctgctcgc gcgcggcgtt gccggcggca tcgcgcgcgc
     2461 gcatgcgcag ggtgtagcgg cccggtgcca ggccggtgaa cacgcggtcg ccgctggcgc
     2521 cgagcgcgac ccaggcgcgg tcgaagccat ccagcctcga ccaatagcgg ttggattcgg
     2581 gatcctcgaa cgcaagcagg cgcgcgcgga tccggaattc gcggtcgtcg cggcccaggc
     2641 gcaggcccgc ctgcagcggg atatcgcgcc attcgccgtt gcgacgcacc gacagccggt
     2701 cgtagcgcag cgaaggtacc gtggccacgg gatccgccgc ggtggtatcg accagcacga
     2761 tgccgccgtc cgcggtcgcc gccgccagca cgccctcggc gctcatcgcc agcgcgtggt
     2821 cgacgaactc ctggctgcca ctgccgtcgc gaatgccatg gcgacgcagg ttgcgggtgc
     2881 gcggatcgta gcggtacagg ccgcgcgagg tggacaccca tgggcgatgg cgcaggtcca
     2941 cccgcatgcc ggatgcgcca accgccggca taccgtgcgc gctgtcgacc cggtccgcgg
     3001 cctgccaacg cccgccgcgc gccacgtagt gcacgagccc ggactgccgc tgcagccaca
     3061 gcatgcggtc gccatcgaag gccagcgcat acacgcgttc gccgccgaac ccgggcaggc
     3121 gccggaacgt gccctgcgcc gcgtccagca ccgccacgcc atccgccccc gccacccaga
     3181 cctcgcctcg cggcgacacc accagcgcct cgatgtcgcc ggtcgccagg ccatgcgcgg
     3241 catccgccgg gatctcgcgc agcacccgcc cgctggcggg gtcgcgctgc tgcacgccgg
     3301 cacccggcgc cgacagccac aggctgccat cgggcgcaac ctgcagctgg tcgacctggc
     3361 caggcggggt cgcgtcgcgc gggtcgccgc tgcgccattc gtcgatcgcc ccgtcactgc
     3421 cgacccggag caggccattg cgatgcccca gccacaggcg cccgccggtg tcttcggcga
     3481 tcgccagcag cttgatgctg cgcaggcgcg ccaggctgtc gtcgtcgagc cggccgatgc
     3541 cgccatccgt cgccagccgc tcgaccatgc cgttgaaccc gcccagccag aacccgccgt
     3601 cgcgcgcggg ggcgagtgcg cggtacatcg ccccctgcag gccatcggtt tcgcccgcgt
     3661 actgcgccaa ttgccgccag tccgggcgca ggtagcccag gcccacgccc gccaccggca
     3721 cccacaacgc gccatcggca tcctgcagca tcgcggtgat gccgcgcggg atgtcggggc
     3781 cgcccaaccg caccgggctc ggcggttccg cgccctgctg ccgccacagc ccgcgctggc
     3841 tggcgatcca gctgccgcca tcgcgcgcgc ggacgatcgc gttcatcgcg ttcgggcggc
     3901 ggaacatcgg cgaccacgcc ggctgcgtcc agtcgccccc cgcatcgcgc cgccagacgc
     3961 cgtccgaggt gccgacccac aggccatcgg cctgcatggt gagcgagtac accagcggcg
     4021 gcgcatcggt ccccggcagg cgcacgccct cgatgcggcc gccctgcatg cgcgccaggc
     4081 cggcatcggt accgatccac agcgcgccgc ccgcatccac cgccagcgca agcacggtat
     4141 cggagggcag gccgtcgctt gccgccgcgt agcgatggat cgtgccgtcg tcgccgatcc
     4201 ggtgcaggcc gccgtcgtag gcaccgaacc agacgctgcc ggcgtgctgc gcgaacgccc
     4261 agatatcgtc gctgcccagt tgcggatggg tggccttgcg gtaatgcacg aagccctgcc
     4321 gttgcgcatc cagcacgctg atgccgccgc cttcggtcgc cacccacacg cggtcgcgcg
     4381 catcgaccag cagcgcctgc acgttgttgc cgggcaggcc ccgcgggtcg cccggctggt
     4441 gccgccacag ccgcatgccg acgccgtcgt ggcgcgccag cccatcggcg gtggcaaccc
     4501 acaggtagcc gtcgcggtcg cgcgcaagcg ccttgatcgc ggtcgaaggc aagccctggc
     4561 cggcaccgac gatgcgcaac cgcggccgct ccggcaccgc cgccagcgcg cacgcgcaca
     4621 gcagccacag tcccagcaag gtgcccaacc tgcgtggcat gcgtgccctc ccctgcccgc
     4681 atgctggcga cccgggttgg ctcatgcaag caggaagcgc cgcaccccct ccggatcgaa
     4741 cggccagtcg agttcgcgcc cgctggcggc atcgcgcagg accggcacgc gcacgccgaa
     4801 gcgcgcttcc agtgccgcgt cgccatcgat gaacacgctc tcgaattcgg gcgtgcgcgc
     4861 ctgcgccagc gcctccaggg cgaggtcgca gaggtggcag tggtcgcgct ggtagaggat
     4921 caggcccata cgcctgcgtc gttcgccgga tggcggtcgc cctagaatag tcgccccagc
     4981 ccccaggcag cgccgcatgg ccgtttccac cttcgacctc tacaagatcg gcatcggccc
     5041 cagttcctcg cacacggtcg gtccgatgcg cgccgccgcg cgcttcgtcg agcaccacct
     5101 ggcggaagcc ggccgcctgc cgcaggtcgc gcgggtgcgc gcggaggtgt tcggatcgct
     5161 ggccctgacc ggccgtggcc acggcaccga caaggcggtg atgatgggcc tggaaggcca
     5221 ttggccgaac cggatcgacc ccgacatcat ccccgcggcg ctgcagcgga tccgcggcga
     5281 aaagcggatc accctgcacg ggacgcacga ggtgggcttc gacgagaagc gcgacctggt
     5341 gatgaacaag cgccagaagc tgccgttcca caccaacggc atgcgcttca tcgcctacga
     5401 cgccgccggc aacgaactcg ccagccgcga ctactactcg gtcggcggcg gcttcgtggt
     5461 caaccaggac gaggccgcgg aggaccgcat cgtcgccgac accaccgtgg tggcgcatcc
     5521 gtatgccagc ggcgacgagc tgctggcacg ctgcgcgcaa tcgggcctgt cgatcgcgca
     5581 gctgatgctt gcgaacgaac gcgcctggcg cagcgacgac gccatccacg ccgggctgcg
     5641 cgagctgtgg caggcgatgc aggactgcgt cgcgcgcggc atccgcgcat ccggcaccct
     5701 gcccggcggc ctgcacgtgg tgcggcgcgc gccgtcgctg cacgcggagc tcgccgccaa
     5761 gccggaggcg gcgatgcgcg acccgctgac cgtgctcgac tgggtcaacc tgtacgcgct
     5821 ggcggtgaac gaggagaacg cggccggcgg gcgcgtggtc accgcgccca ccaacggcgc
     5881 cgccggcatc atcccggcgg tgctgcacta ctacgaccgc ttcatccccg gcgccaacga
     5941 gcagggggtg ttcgacttcc tgctgaccgc cgccgcggtc ggcatcctgt acaaggaaaa
     6001 cgcctcgatc agcggcgccg aggtcggctg ccagggcgag gtcggggtgg cctgctcgat
     6061 ggccgcggcc ggcctggccg ctgcgctcgg cggcagcccg gggcagatcg agaacgccgc
     6121 cgagatcggc atggagcaca acct
//
LOCUS       DAORUK010000126         6062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_15477_length_6062_cov_16.214583, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000126 DAORUK010000000
VERSION     DAORUK010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15477_length_6062_cov_16.214583"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PK818_08370"
     CDS             complement(<1..451)
                     /locus_tag="PK818_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005413670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HQY82782.1"
                     /translation="METDLLPDPRITCHVVDIAEGRLDPAAAIEFVSDDAHGGIDVFI
                     GRVRASSHGRQCVAIHYDMFDPLALTVFRSTAAAAVERFGPKAKCYVAHARGKLAVGD
                     LAVVIAFATPHRDEAFRGCREVIEAVKHQAPIWKQEHFIDGHSEWSEG"
     gene            complement(454..714)
                     /locus_tag="PK818_08375"
     CDS             complement(454..714)
                     /locus_tag="PK818_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HQY82783.1"
                     /translation="MKLTVQLFGRFREFDAAPEITLDLPGIATVADFRAAFDAWAGAH
                     WPGYAPGLLKASAIATASTLLRADSPLPADGRLALLPPVSGG"
     gene            complement(711..1970)
                     /locus_tag="PK818_08380"
     CDS             complement(711..1970)
                     /locus_tag="PK818_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HQY82784.1"
                     /translation="MKTMLSVDEALAMIRECCTPLPVESVPLADACGRVLAAAVDAPR
                     DLPPFANSAMDGFALRWREGLVEDTELPVLAEQAAGDGIAGQSDGACSIMTGARIPDG
                     LDTVVPVEDCTVLARDGEGRPVSIALSHAPQPGQHVRHAGEDVAVGQRVLETGVRLDE
                     EALLVLRGIGIGTVMVHRRPRLALACTGRELVDVDGAELAPGQIANTNGPYLERLFAE
                     AGAEVVERVTLPDEPAVFAAWLTRWLEAGIELVVTTGAVSMGRYDFVPDVLREAGADL
                     RFHKLKMRPGKPLLFATAGRGTLVFGLPGNPVSSTVGARFFVDAALRVLGGRSPEAAL
                     LLPLAAAARKKPGFSMLQKAALALDAGGRLQVRLLKGQESFKTAPLLAARAWAVLPQD
                     AAELPAGTPVPVYPLRPGGAPFDGLIR"
     gene            complement(1967..2968)
                     /gene="moaCB"
                     /locus_tag="PK818_08385"
     CDS             complement(1967..2968)
                     /gene="moaCB"
                     /locus_tag="PK818_08385"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014647394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HQY82785.1"
                     /translation="MDAPTPASGFHMADVRRKRPSERRAVAVGELRAGPVGFAALAGK
                     TLPKGDALAMAEVAGLQGAKNAAQLMPLCHPLSLEYVDVRCVPVPERHAIRVYCETSL
                     TAKTGVEMEALAGASAALLTLYDLTKPVEPALTIEAVRLLFKEGGKKGLWIHPAGMSD
                     DERTYYRPQSDPRLDGESVAVITLSDRAAAGVYEDKSGALLEQRLQAMGADVHRRLLP
                     DGIEPLADALRQEAARGTPLVLCTGGTGFGPRDLAPEALAQVATRPVPGLAELFRNAS
                     SAHTPLAWLSRNTCALVGDGTLVVLLPGSPKAVAQGMDILAPLLPHALAMIRGLPHA"
     gene            complement(2969..3952)
                     /gene="moaA"
                     /locus_tag="PK818_08390"
     CDS             complement(2969..3952)
                     /gene="moaA"
                     /locus_tag="PK818_08390"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012511281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HQY82786.1"
                     /translation="MAILEDRFGRSFPYLRLSVIEACNFRCTYCLPDGFQARAGRPQP
                     LTREEIARLLRGFAGVGLRKLRLTGGEPTLRRDLPEIIASAAAVGGIDSIALTTNGTL
                     LDRRVARWREAGLTNLNVSVDALDRERFHAITGHDRLAEILRGIELARGMGFKAVKLN
                     AVLLKGLNDDQLPAWLDYLRERDVSVRFIELMRTGDNLAYFERHHLRAEALETQLQEA
                     GWRLLPRAPDAGPAREYAHPGYAGRIGVIAPYSKDFCAGCNRLRVTHTGDLRLCLFGN
                     VGIPLRPWLQDDGDRDRLVAALQAQLGLKAIGHRLHEGETGMTPNLSTIGG"
     regulatory      complement(3946..4079)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(4263..5474)
                     /locus_tag="PK818_08395"
     CDS             complement(4263..5474)
                     /locus_tag="PK818_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HQY82787.1"
                     /translation="MRAALPLALAVALGGLAAAPTPALAAGPAQAAPKPWALNLRYRH
                     ESVDDDAFARTAEADTLRLRATFNHRFDPHWTAQVEAEGVAELGDRFNSSANGQVQYP
                     LVPDARALELNQAWLDWSGDKTGVRVGRQMLVLDNARFVGNVAWRQNMQTFDSAQLRW
                     KPSKAIEVQALYLDRVHRINGDEARDPLARERRLKAPLLRVSHAVPGGSLVGYGYWLE
                     DRDVASASTRSLGLRWNASRALSTDWKGGLVLERAQQVPWADAVGGSTAYMLVEPRLE
                     RGPLLLKAGWERLGAGRGRAFQTPLATLHAFNGWADKFLATPVNGLRDTYVSAQRGIE
                     VGGRKGKWEVAYHDFRGDRGGMDYGRELDASLGVTLRPGLDVLAKLASYQSEGFARDT
                     DKLWLQLEWSL"
     gene            complement(5572..5913)
                     /locus_tag="PK818_08400"
     CDS             complement(5572..5913)
                     /locus_tag="PK818_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82788.1"
                     /translation="MLQAYFDDRVPGNLRLARVFWLEGVLASHVLFLALLAIYQYGPA
                     IALVAAVAGFMAYTAWIMRRIWLNAPNVERAELGTMARVLTIGWTINAVTVCTFLVLA
                     KLGGQPLQFLG"
BASE COUNT          893 a         2179 c         2124 g          866 t
ORIGIN      
        1 agccctcgct ccattcgctg tggccgtcga tgaaatgctc ctgcttccag atcggcgcct
       61 ggtgcttgac cgcttcgatg acctcgcggc agccgcggaa ggcttcgtcc cggtgcggcg
      121 tggcgaacgc gatcaccacc gccaggtcgc cgaccgccag cttgccgcgg gcatgggcga
      181 cgtagcactt cgccttcggg ccaaaacgct cgaccgccgc ggcggcggtg ctgcggaaca
      241 ccgtgagcgc cagcggatcg aacatgtcgt agtggatggc gacgcactgg cgtccgtggc
      301 tggacgcgcg cacgcggccg atgaacacgt cgatgccgcc gtgggcatcg tcgctgacga
      361 actcgatcgc cgccgccgga tccagccggc cctcggcgat gtcaactacg tggcaggtga
      421 tgcgcggatc cgggagcagg tcggtctcca tgctcagcct ccgctcactg gcggcagcaa
      481 cgccagccgg ccatcggcgg gcagcgggct gtcggcgcgc aacagggtgg atgcggtggc
      541 gatcgccgaa gccttcagca ggcccggcgc gtaacccggc cagtgcgcgc cggcccacgc
      601 gtcgaatgcg gcgcggaagt ccgcgacggt ggcgatgcca ggcaggtcca gcgtgatctc
      661 gggggctgca tcgaattcgc ggaagcggcc aaacagttgc acggtgagtt tcatcggatc
      721 agtccatcga acggggcgcc gccggggcgg agcgggtaca ccggcacggg cgtcccggcg
      781 ggaagctcgg cggcgtcttg gggcagcacg gcccaggcgc gcgcagccag cagcggcgcg
      841 gtcttgaagc tttcctggcc tttcaggagg cgcacctgca ggcggccacc ggcatccagc
      901 gccagtgcgg ccttctgcag catggaaaag ccgggtttct tgcgcgcggc ggcggccagc
      961 gggagcagca gtgccgcttc cggcgatcgg ccgcccagca cacggagcgc ggcatcgacg
     1021 aagaagcgcg cgcccacggt gctcgacacc ggattgcccg gcaggccgaa caccagggtg
     1081 ccgcggccag cggtggcgaa cagcagcggc ttgcccgggc gcatcttcag cttgtggaag
     1141 cgcaggtccg cgccggcctc gcgcaacacg tccggcacga agtcgtaacg gcccatcgac
     1201 accgcgccgg tggtgacgac gagttcgatc ccggcctcca gccagcgcgt cagccaggcc
     1261 gcgaagacgg cgggttcgtc gggcagggtg acccgttcga ccacctcggc gccggcctcg
     1321 gcgaacagcc gttcaaggta cgggccgttg gtgttggcga tttgtccggg ggcgagctcg
     1381 gcaccatcga catcgaccag ttcgcggcca gtgcaggcca gcgccaagcg cggccggcgg
     1441 tgcaccataa ccgtgccgat gccgatcccg cgcagcacca acagcgcttc ctcgtccagc
     1501 cgcacgcccg tctccagcac gcgctggccg acggccacgt cctcgcccgc atgccgcacg
     1561 tgctgtcccg gctgcggcgc atgcgagagc gcgatcgaca ccgggcggcc ttcgccatcg
     1621 cgcgcgagca ccgtgcagtc ctcgaccgga accaccgtgt ccagcccgtc cgggatacgc
     1681 gcgccggtca tgatgctgca ggcgccgtcg gattggccgg cgatgccgtc gccagcagcc
     1741 tgttcggcca gcactggcaa ctccgtgtcc tcgaccaggc cttcgcgcca gcgcagggcg
     1801 aacccgtcca tcgccgagtt ggcgaatggc ggcaggtcgc gcggcgcgtc caccgccgcc
     1861 gccaacaccc ggccacaggc gtctgccagc ggcaccgatt cgaccggcaa cggcgtgcag
     1921 cactcgcgga tcatcgccag cgcctcgtcc acggagagca tcgtcttcat gcgtgcggca
     1981 acccgcggat catcgccagt gcatgcggca gcagcggggc gaggatatcc atgccttgcg
     2041 ccaccgcctt cgggctgccc ggcagcagga ccaccagggt gccgtcgccg accaacgcgc
     2101 aggtgttgcg gctcagccat gccagcgggg tgtgcgcgct gctggcattg cggaacagtt
     2161 cggcgagtcc cggcaccggg cgggtggcga cctgtgccag cgcctccggt gccaggtcgc
     2221 gcggaccgaa gccggtgccg ccggtacaca gcaccagcgg ggtgccgcgc gcagcttcct
     2281 ggcgcagcgc atcggccagc ggctcgatgc cgtccggcag caaccggcgg tgcacgtccg
     2341 cacccatcgc ctgcaaccgc tgttccagca gagcaccact cttgtcttcg tacacgccgg
     2401 cggcggcgcg atcgctcagg gtgattaccg cgaccgattc gccatcgagc cgcggatcgg
     2461 attgcgggcg gtagtacgtg cgttcgtcgt cgctcatgcc cgccggatgg atccacaggc
     2521 ccttcttgcc gccttccttg aacagcaggc gcaccgcctc gatggtcagc gccggctcga
     2581 ccggcttggt caggtcgtac aacgtgagca gggcggcgct ggcgccggca agcgcttcca
     2641 tctcgacacc ggtcttcgcg gtgagcgagg tctcgcaata cacgcggatc gcgtgccgct
     2701 cgggtaccgg cacgcagcgc acgtccacgt attccagcga cagcggatgg cacagcggca
     2761 tcagttgcgc ggcgttcttc gcgccctgca ggccggccac ttccgccatc gccagcgcat
     2821 cgcccttggg cagggtcttg ccggccagcg cggcgaagcc gaccggcccc gcgcgcagtt
     2881 cgccgaccgc caccgcgcgc cgctcgctgg ggcgcttgcg gcggacgtcg gccatgtgga
     2941 agccggaggc gggggtgggt gcgtccattc agccgccgat ggtggaaagg ttgggggtca
     3001 tgccggtctc gccctcgtgc aggcggtggc cgatcgcctt caggcccagc tgcgcctgca
     3061 gtgcggcgac caggcggtcg cggtcgccgt cgtcctgcag ccatggccgc agcgggatgc
     3121 cgacgttgcc gaacaggcac agccgcaggt cgccggtgtg ggtcacccgc aggcggttgc
     3181 agcccgcgca gaagtccttc gagtacggcg cgatcacccc gatccgcccg gcgtagcccg
     3241 gatgcgcgta ttcgcgcgcc gggccggcat cgggcgcgcg cggcagcagg cgccagccgg
     3301 cctcctgcag ctgggtctcc agcgcctccg cacgcaggtg gtggcgctcg aagtaggcga
     3361 ggttgtcgcc ggtgcgcatg agttcgatga agcgcacgct gacgtcgcgc tcgcgcaggt
     3421 agtccagcca cgccggcagc tggtcgtcgt tgaggccctt cagcagcacc gcgttgagct
     3481 tgaccgcctt gaagcccatc ccgcgcgcca gctcgatccc gcgcaggatc tccgccaggc
     3541 ggtcgtggcc ggtgatggcg tggaagcgct cgcggtccag cgcatccacg ctgacgttga
     3601 ggttggtcag cccggcctcg cgccagcgcg ccacccggcg gtcgagcagg gtgccgttgg
     3661 tggtcagggc gatgctgtcg atgccgccca ccgccgccgc ggaggcgatg atctcaggca
     3721 ggtcgcggcg cagggtgggc tcgccgccag tcagccgcag cttgcgcagg ccgaccccgg
     3781 cgaatccgcg cagcagccgc gcgatctcct cgcgggtgag cggctgcggc cgcccggcac
     3841 gcgcctggaa gccatccggc aggcagtagg tgcaacggaa attgcaggcc tcgatcaccg
     3901 agagccgcag gtacgggaag ctccggccga accggtcctc caggattgcc atcaggttgt
     3961 gctcctgtcc aagcgcgggc ggcgacaccg tttccagtgg caccgggtgg cgggatccgc
     4021 cacggtcgcg ggccatgcca gggtggcgaa ggccatgcga ctcggagtgc agcgcgagct
     4081 gcggcgccag tctaatccag cgcccgcggc accgacattg attcggatca atcgccggcg
     4141 gcggtcttca cccgtgccgg ctatcgatcc gcggcttccg cgccggcgcg cgcgacacca
     4201 catcggccgc agcccatgca acctctaggt gcggggcttg ccccgcacgg acgcgcgtcc
     4261 gcctacaggc tccactccag ctgcagccac agcttgtcgg tatcgcgcgc gaagccctcg
     4321 ctctggtaac tggcgagctt ggccagcacg tcaagacccg ggcgcagggt cacgcccagc
     4381 gaggcgtcaa gctcgcggcc atagtccatg ccgccgcggt cgccgcggaa gtcgtggtag
     4441 gccacttccc acttgccctt gcggccgccg acctcgatcc cgcgctgcgc cgagacatag
     4501 gtatcgcgca ggccgttgac tggcgtggcc aggaacttgt cggcccagcc gttgaaggcg
     4561 tgcagggtcg ccagcggggt ctggaaggcg cggccgcggc cggcgccaag ccgttcccag
     4621 ccggccttga gcagcagcgg gccacgttcc agccgcggtt ccaccaacat gtacgcggtc
     4681 gagccgccca ccgcatccgc ccacggcacc tgctgcgcgc gttccagcac cagcccgccc
     4741 ttccagtccg tcgacagcgc gcggctggca ttccagcgca ggcccaggct gcgggtggag
     4801 gcgctggcca cgtcgcggtc ctccagccag tagccgtagc cgaccaggct gccgcccggc
     4861 accgcgtgcg aaacccgcag cagcggcgcc ttcagccggc gctcgcgcgc cagcgggtcg
     4921 cgcgcctcgt cgccgttgat ccggtgcacg cggtccaggt agagcgcctg cacctcgatc
     4981 gccttgctcg gcttccagcg cagctgcgcg gaatcgaagg tctgcatgtt ctgccgccag
     5041 gccacgttgc cgacgaagcg ggcgttgtcc agcaccagca tctgccggcc cacgcgcacc
     5101 ccggtcttgt cgccggacca gtccagccag gcctggttga gttccagcgc gcgcgcgtcc
     5161 ggcaccagcg ggtactggac ctggccgttg gcgctggaat tgaagcggtc gcccagttcg
     5221 gccacgccct cggcctcgac ctgcgcggtc cagtgcggat cgaagcggtg gttgaaggtt
     5281 gcgcgcaggc gcagggtgtc ggcttcggcg gtacgggcga aggcgtcgtc gtcgacgctc
     5341 tcgtggcggt agcgcaggtt cagcgcccat ggcttgggcg ccgcctgggc gggaccggca
     5401 gcgagcgccg gggtgggggc cgcggccagg ccacccaggg ccaccgcgag tgcaagggga
     5461 agggcagcac gcatggaacg ctccggcagt ttcatctgcc ggaaattgtc cgcaggtggg
     5521 gccggtgccg ccttgtctca ggtcaaggcg acggaagcga ggatgccgcg ttcagcccag
     5581 gaactgcagc ggctgcccgc ccagcttcgc cagcacaagg aaggtgcaca cggtcaccgc
     5641 attgatggtc cagccgatgg tcagcacccg cgccatggtg cccagttccg cccgttccac
     5701 gttgggcgcg ttcaaccaga tccggcgcat gatccacgcg gtgtaggcca tgaaccccgc
     5761 caccgcggcc accagcgcaa tcgccgggcc gtactggtag atcgcaagca gcgccaggaa
     5821 cagcacatgg ctggccagca cgccttccag ccagaacacc cgcgccagcc gcaggttgcc
     5881 tggaacgcga tcatcgaagt aggcctgcag catgggcatc tccattggcg tggtcgtccg
     5941 aaccggttgt cgccacgtta gccccgccgc ggcctgcagg caactgccgt gcgcggcggg
     6001 ccggggccgc cgccatcatg caaaaggccc gcttgcgcgg gccccttgca acatcacgtg
     6061 cc
//
LOCUS       DAORUK010000127         5995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_15703_length_5995_cov_19.273401, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000127 DAORUK010000000
VERSION     DAORUK010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15703_length_5995_cov_19.273401"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..918
                     /locus_tag="PK818_08405"
     CDS             151..918
                     /locus_tag="PK818_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004090267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HQY82789.1"
                     /translation="MRSGNPALKESTFLDLGTGTLVRNDAGAMSLNGTVNKTALLLVL
                     TLAGALFTWSQFSAAMAAGNPGAVMPYVWGGAIGGFVVALVTVFRKEWAMYTAPLYAV
                     LEGLFLGAVSAMFELRFPGIVMQAVGLTFGTMAALLMAYRSGLIKVTENFKLGIVAAT
                     GGIALLYLVNIGMRLFGFEGMAFIHEGSLLGIAFSGFVVVIAALNLVLDFDFIETGVE
                     QGAPKYMEWYAAFGLLVTLVWLYLEMLRLLSKLQSRN"
     gene            complement(1013..2017)
                     /locus_tag="PK818_08410"
     CDS             complement(1013..2017)
                     /locus_tag="PK818_08410"
                     /EC_number="1.1.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020852046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase"
                     /protein_id="HQY82790.1"
                     /translation="MTTITIIRGDGIGPEIMDATLHVLDAMQLGLAYEFADAGLAALE
                     KHGELLPAATMDSIRRNSVALKSPLTTPVGEGFSSINVELRKRFDLYANVRPAKSFPN
                     TKSRFPAGVDLVIVRENTEGAYSGEGQQVSEDGETATLIQKITHRGSERIVRYAFELA
                     RKTGRKKVTVVHKANILKSTSGLFLKTAREVAALYPDIQCNEMIVDNTCMQLVMRPEQ
                     FDIIVTTNLFGDIISDLCAGLVGGLGLAPGANIGTDAAIFEAVHGSAPDIAGKGIANP
                     CALLLGAAQMLDHLARPEQAERLRKAIIGTLEAKDSLTPDLGGEGNTMSFAQAIASRL
                     "
     gene            complement(2128..2511)
                     /locus_tag="PK818_08415"
     CDS             complement(2128..2511)
                     /locus_tag="PK818_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HQY82791.1"
                     /translation="MSGGDRVSEFTEFRQRMNKRILDEPNQVVRRFFALDTQAYQAGA
                     LDVKTKELMGLTASMVLRCDDCISYHVAQCKDAGVTREEMFEAFSVGLVVGGSIVIPH
                     LRRAVDFLDRLEQGEAQAPATHDHG"
     gene            complement(2508..2786)
                     /gene="grxC"
                     /locus_tag="PK818_08420"
     CDS             complement(2508..2786)
                     /gene="grxC"
                     /locus_tag="PK818_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HQY82792.1"
                     /translation="MSDSSPPGITIYSSAICPYCVAAKNFLKSKGLAWTEVRIDTDPA
                     QREAMVQKTRRTSVPQIFIGDTHVGGYDDMMALHRAGKLEPLLAGGGA"
     gene            2906..4537
                     /locus_tag="PK818_08425"
     CDS             2906..4537
                     /locus_tag="PK818_08425"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HQY82793.1"
                     /translation="MTRPRFRRRWPAALAFALCCATASAQQARLPDIGSSAGAVLSPA
                     RQAEYGSMVLAQLRHEGYILDDPLMDGWLRDVGLRLAGASDRPQQGFTFFLLRDRQIN
                     AFATLGGYIGMNAGLVLAADREDQVAGVLAHEIAHVTQQHVLRGAEKAQRDSLPILLA
                     TLGAIIAAQQAGGNSSGDASQAALVTGLGLLQQRQIDYTRDNEAEADRVGIRTLANGG
                     FAPEAMAEFFETLQAVVRVNQGDERSRVPGYLQTHPVTLSRISEARERAAKLEVGNAT
                     GSTAIATNPLLPAGLRIQTQGARSRGTTGDFGWARERLRVLSANTPAQAIGEYRQMQA
                     SKPLDDAQRYGLAYAHLRAGDATTALQELQPVQAAHPDSLWLQISLGETEARAGRTAD
                     ADRRFETLLGRMPTHRALALSYARVLAERNTKASGARAVAVLRPLLATAASDALFQQS
                     YARANEIAGDDIRAGEAWAEAAYLGGRPEQALVQLNNLKKRANLDYYARSRIDARIAA
                     ITPTVLELRRQGVHDEEATGKWRVGLRAGEPAGAW"
     gene            4627..5328
                     /gene="phoB"
                     /locus_tag="PK818_08430"
     CDS             4627..5328
                     /gene="phoB"
                     /locus_tag="PK818_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HQY82794.1"
                     /translation="MQKRILIVDDEPAIRDMVAYALRKGDFDPVLAGDAREAQAAISE
                     RVPDLILLDWMLPGTSGLELARRWRREALTRDIPIIMLTARGEENDRVGGLEAGVDDY
                     VVKPFSARELLARIRAVMRRSREDDEDGSVQVGGLRIDGAAHRVFADGSAGPTPVHIG
                     PTEYRLLHFFMTHPERVYSRSQLLDHVWGGSVYVEERTVDVHIRRLRKTLEPHRLDPM
                     VQTVRGSGYRFSAAA"
     gene            5376..>5995
                     /locus_tag="PK818_08435"
     CDS             5376..>5995
                     /locus_tag="PK818_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017158112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3329 domain-containing protein"
                     /protein_id="HQY82795.1"
                     /translation="MSPDARNAWFRTLGQLALVLAAGLLVGLLLGQPWPTLTVAALGV
                     VAWHYWKLRHVLLRLTARQRLEPPQGVGVWNELDRLLFRSQSEMRARKRRLLAMLRAY
                     RAAAAALPDAVVVVERNSQRVLWFNEAAGPLLGLQFPRHQGSSIVGALQPLPMAHWLA
                     AGRNAEPMTDVASPADPDLRLGLRLIPYSDELWLLVARDVTRTMRL"
BASE COUNT          891 a         2135 c         2054 g          915 t
ORIGIN      
        1 gatttaggtt ccagtgccgc aaggcgtgcg ggttcgagtc ccgcctttcg caccagccac
       61 gcatccaggc cgccgtcggc aaacgcgccc gccccgcgat atgctccgcg catgcggcga
      121 ccgccgcgct tccgaggacc cacgaccatg atgcgcagcg gcaatcccgc actgaaggaa
      181 agcaccttcc tcgacctcgg caccggcacc ctggtgcgca acgacgccgg ggcgatgagc
      241 ctgaacggca ccgtcaacaa gaccgcgctg ctgctggtgc tgaccctggc cggcgcgctg
      301 ttcacctggt cgcagttctc ggccgcgatg gccgccggca accccggcgc ggtgatgccg
      361 tatgtctggg gcggcgcgat cggcggcttc gtggtcgcgc tggtcacggt gttcaggaag
      421 gaatgggcga tgtacaccgc gccgctgtac gcggtgctgg aaggcctgtt cctgggcgcg
      481 gtctcggcaa tgttcgagct gcgcttcccg ggcatcgtga tgcaggcggt tggcctcacc
      541 ttcggcacca tggcggcgct gctgatggcc taccgcagcg ggctgatcaa ggtgaccgag
      601 aacttcaagc tcggcatcgt cgccgccacc ggcggcatcg ccctgctgta cctggtcaac
      661 atcggcatgc gcctgttcgg gttcgagggc atggccttca tccacgaggg cagcctgctc
      721 ggtatcgcgt tctccggctt cgtggtggtg atcgcggcac tcaacctggt gctggatttc
      781 gacttcatcg agacgggcgt ggaacagggc gcgccgaagt acatggagtg gtacgccgcg
      841 ttcggcctgc tggtcacgct ggtgtggctg tacctggaga tgctgcgcct gctcagcaag
      901 ctgcaatcgc gcaactgatc gcgctttcga tgcaacgaaa gaagggcgcc gctggcgccc
      961 ttctgcattt tcgacaccgc tgccggcggc aggcgccggc ggcgacccgc gcctacagcc
     1021 gcgacgcgat cgcctgcgcg aaagacatcg tgttgccctc gccgccgagg tccggggtca
     1081 gcgaatcctt cgcttccagg gtgccgatga tcgccttgcg caggcgctcg gcctgctccg
     1141 gccgggccag gtggtcgagc atctgcgccg cgcccagcag cagggcgcac gggttggcga
     1201 tgcccttgcc ggcgatgtcc ggggccgagc cgtggaccgc ctcgaagatc gccgcgtcgg
     1261 tgccgatgtt ggcgcccggg gccagcccca ggccaccgac caggcccgcg cacaggtcgg
     1321 agatgatgtc gccgaacagg ttggtggtga cgatgatgtc gaactgctcc ggccgcatca
     1381 ccagctgcat gcaggtgttg tcgacgatca tctcgttgca ctggatgtcc gggtacagcg
     1441 cggcgacttc gcgtgcggtc ttcaggaaca ggccggaggt cgatttcagg atgttggcct
     1501 tgtgcaccac cgtcaccttc ttgcggccgg tcttgcgggc aagctcgaac gcatagcgga
     1561 cgatccgctc ggagccgcga tgggtgatct tctggatcag ggtggcggtc tccccgtcct
     1621 ccgagacctg ctggccttcg ccggaatacg cgccctcggt gttctcgcgg acgatgacca
     1681 ggtcgacgcc ggccggaaag cgcgacttgg tgttcgggaa gctcttggcc gggcgcacgt
     1741 tggcgtacag gtcgaagcgc ttgcgcaact cgacgttgat cgatgagaag ccctcgccga
     1801 ccggcgtggt cagcggcgac ttcagggcca cgctgttccg gcggatcgag tccatggtcg
     1861 ccgccggcag cagctcgccg tgcttctcca gcgcggccag gccggcgtcg gcgaattcgt
     1921 acgcaaggcc aagctgcatc gcatccagca cgtgcagggt ggcgtccatg atttccgggc
     1981 cgatgccgtc gccgcggatg atcgtgatgg ttgtcatttt tgcaggattc cagacaggct
     2041 gaacgccacg cacggggcgt ggcgtcatgg ggatttacag cccgccatta tggcagatgg
     2101 cgtgcgccgc cgtatccgcc ggcgcattca accatggtcg tgggtggccg gtgcctgcgc
     2161 ctcgccctgc tccagccggt cgaggaaatc gaccgcgcgg cgcaggtgcg ggatcacgat
     2221 cgatccgccc accaccagcc ccaccgagaa cgcctcgaac atctcctcgc gggtcacgcc
     2281 ggcgtccttg cactgggcga cgtggtagct gatgcagtcg tcgcagcgca gcaccatcga
     2341 cgcggtcagc cccatcagct ccttggtctt cacgtccagc gcgccggcct ggtaggcctg
     2401 ggtatcgagc gcgaagaagc gccgcaccac ctggttgggc tcgtccagga tgcgcttgtt
     2461 catgcgctgg cggaattcgg tgaattcgct gacccggtcg ccgccgctca tgcgccgccc
     2521 ccggccagca gcggttcgag cttgccggcg cgatgcagcg ccatcatgtc gtcgtagccg
     2581 ccgacgtggg tgtcgccgat gaagatctgc ggcacgctgg tgcggcgggt cttctgcacc
     2641 atcgcctcgc gctgggcggg atcggtgtcg atcctgacct cggtccacgc caggcccttg
     2701 ctcttcagga aattcttggc cgccacgcag tacgggcaga tcgcgctgct gtagatggtg
     2761 atgccggggg ggctgctgtc gctcaagacg gactccggga acctgcaagg atgctggcgg
     2821 tcgaatatgc agccgggcgc cgcgtttttc cagccgctca acccgcattc acaccaagcg
     2881 caccacaaca cagcatcctg cccgcatgac ccgaccccga ttccgccgcc gctggcccgc
     2941 cgcgctcgcc ttcgccctgt gctgcgcgac ggcgagcgcg cagcaggccc ggctgccgga
     3001 catcggctcg tccgccggcg cggtgctgtc gccggcgcgg caggccgaat acggctcgat
     3061 ggtgctggcc cagctgcggc acgagggcta catcctcgac gacccgctga tggacggctg
     3121 gctgcgcgat gtcggcctgc gcctggccgg cgccagcgac cgcccgcagc agggcttcac
     3181 cttcttcctg ctgcgcgacc gccagatcaa cgcctttgcc accctcggcg gctacatcgg
     3241 catgaacgcc gggctggtgc tggccgcgga ccgcgaggac caggtcgccg gcgtgctcgc
     3301 ccacgagatc gcccacgtca cccagcagca cgtcctgcgc ggtgccgaaa aggcgcaacg
     3361 cgatagcctg ccgatcctgc tcgccaccct cggcgcgatc atcgccgcgc agcaggccgg
     3421 cgggaactcc agcggcgatg ccagccaggc cgcactggtg accgggcttg gcctgctgca
     3481 gcagcgccag atcgactaca cccgcgacaa cgaggccgag gccgaccgcg tcggcatccg
     3541 caccctcgcc aatggcggct tcgcgccgga ggcgatggcg gaattcttcg agaccctgca
     3601 ggcggtggtg cgggtgaacc agggcgacga gcgcagccgc gtccccggct acctgcagac
     3661 ccacccggtg accctcagcc ggatcagcga ggcacgcgaa cgcgcggcca agctggaggt
     3721 cggcaacgcc accggcagca ccgccatcgc caccaatccg ttgctgcccg ccggcctgcg
     3781 gatccagacc cagggcgcgc gcagccgcgg caccaccggc gatttcggct gggcgcgcga
     3841 gcgcctgcgc gtgctcagcg ccaacacccc ggcgcaggcg atcggcgaat accggcagat
     3901 gcaggccagc aagccgctcg acgacgccca gcgctacggc ctggcctacg cccacctgcg
     3961 cgccggcgac gccaccaccg cgctgcagga actgcagccg gtgcaggccg cgcaccccga
     4021 cagcctgtgg ctgcagatca gccttggcga aaccgaggcc agggccgggc gcacggcgga
     4081 cgcggacagg cgcttcgaga ccctgctggg gaggatgccg acccaccgcg cgctggcgct
     4141 gagctacgcg cgggtactgg ccgaacgcaa caccaaggcg agcggtgcgc gtgcggtcgc
     4201 ggtgctgcgg ccgctgctgg ccaccgccgc cagcgacgcc ctgttccagc agtcgtatgc
     4261 acgcgccaac gagatcgccg gcgacgacat ccgcgccggc gaggcatggg cggaggcggc
     4321 ctacctcggc ggccggccgg aacaggcgct ggtgcagctg aacaacctga agaagcgcgc
     4381 caacctggac tattacgcgc gcagccggat cgacgcgcgg atcgccgcga tcaccccgac
     4441 cgtgctggaa ctgcgccggc agggcgtgca tgacgaggaa gccaccggca aatggcgggt
     4501 cggcctgcgc gcgggcgagc ccgcgggcgc ctggtgaccc ggcgcaggtg acaacgtcac
     4561 aaagcggtca taaaaccgtc gtgtaatggc aggatagccg ctcccgcggt gcctacgtgc
     4621 cctgccatgc aaaagcgcat cctcatcgtc gacgacgaac ccgcgatccg cgacatggtc
     4681 gcctacgccc tgcgcaaggg cgacttcgac ccggtcctcg ccggcgatgc gcgcgaggcc
     4741 caggccgcga tctccgaacg cgtgccggac ctgatcctgc tggactggat gctgcccggc
     4801 accagcggcc tggaactggc gcggcgctgg cggcgcgagg cgctgacccg cgacatcccg
     4861 atcatcatgc tgaccgcgcg cggcgaggaa aacgaccgcg tcggcgggct cgaggccggg
     4921 gtcgacgact acgtggtcaa gccgttctcg gcgcgcgagt tgctggcccg catccgcgcg
     4981 gtgatgcgcc gctcgcgcga ggacgacgag gacggcagcg tccaggtcgg cggcctgcgc
     5041 atcgacggcg ccgcccaccg ggtgttcgcg gatggcagcg ccggaccgac cccggtgcac
     5101 atcggtccca ccgaataccg cctgctgcac ttcttcatga cccacccgga gcgggtgtac
     5161 agccgttcgc agctgctgga ccacgtctgg ggcggcagcg tgtacgtcga ggagcgcacc
     5221 gtcgacgtgc acatccggcg cctgcgcaag accctggagc cgcaccggct cgacccgatg
     5281 gtgcagaccg tgcgcggctc cggttatcgg ttctcggcgg ccgcctgaca tcccgccgca
     5341 acccaaccgg cgcgtccagc gcctagactg gccgcatgtc ccccgacgcc cgcaacgcct
     5401 ggttccgcac cctcggccag ctcgccctcg tgctggccgc cggcctgctg gtcgggctgc
     5461 tgctcggcca gccatggccg acgctgaccg tggccgcgct cggcgtggtc gcctggcact
     5521 actggaagct gcgccacgtg ctgctgcgcc tgaccgcgcg ccagcggctc gaaccgccgc
     5581 agggcgtggg cgtgtggaac gagctcgacc gcctgttgtt ccgcagccag tccgagatgc
     5641 gcgcgcgcaa gcgccgcctg ttggcgatgc tgcgcgccta ccgggccgcg gcggccgcat
     5701 tgccggacgc ggtggtggtg gtcgaacgca acagccagcg cgtgctctgg ttcaacgagg
     5761 cggcgggtcc attgctcggc ctgcagttcc cgcgccacca gggcagctcg atcgtcggcg
     5821 ccctgcagcc gttgccgatg gcgcactggc tggccgccgg gcgcaatgcc gagccgatga
     5881 ccgacgtcgc ctcgccggcc gaccccgacc tgcgcctggg cctgcgcctg atcccgtatt
     5941 ccgacgagct gtggctgctg gtcgcgcgcg acgtgacccg caccatgcgc ctgga
//
LOCUS       DAORUK010000128         5909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_16063_length_5909_cov_22.238469, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000128 DAORUK010000000
VERSION     DAORUK010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16063_length_5909_cov_22.238469"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="PK818_08440"
     CDS             complement(<1..152)
                     /locus_tag="PK818_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HQY82796.1"
                     /translation="MLFLLFACGNLFAQDAAPAPTISKADTVWVLVSAALVIFMTLPG
                     LALFYGG"
     gene            complement(211..549)
                     /locus_tag="PK818_08445"
     CDS             complement(211..549)
                     /locus_tag="PK818_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HQY82797.1"
                     /translation="MKLITAIIRPFKLDEVREALTEVGVSGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKLKIECAVADGDFDAALDAISKSAATGKVGDGKIFVVALDHVIRI
                     RTGELGADAL"
     gene            complement(563..1405)
                     /locus_tag="PK818_08450"
     CDS             complement(563..1405)
                     /locus_tag="PK818_08450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HQY82798.1"
                     /translation="MSSHTVATPFHASTTTGILSNGCTHTRTTTTRSTRTRLAMALLA
                     LFGIADAQAGTTGNVSLTSDYVFRGVSQSNSDPALQGGVEFSAESGAYIGAWGSSISW
                     LSDLSTTAAPLSSSLELDVYGGYRGTFSDAVSYDVGALYYWYPGDFPAGFNSADTLEV
                     YAGITVAASEKISLGAKYSVSTTDLFGYVDSGGSGYLDLTANLAVAEGWTIGAHAGRQ
                     WIAGNDAFEYTDWKLGVTRAFDNGFSVGLAYTGTDADDALYTNPFGNKVADDTVALAI
                     TKAF"
     gene            complement(1711..3120)
                     /gene="glnA"
                     /locus_tag="PK818_08455"
     CDS             complement(1711..3120)
                     /gene="glnA"
                     /locus_tag="PK818_08455"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HQY82799.1"
                     /translation="MSFENVEKLIRDHKVEFVDLRFTDLRGVQHHVTFPKSIVDASLF
                     EDGKMFDGSSISGWKGINESDMVLLPDASTAVIDPFTADPTLILTCDILDPATMQAYS
                     RDPRGVAKRAEAFLKASGIADTAFFGPEPEFFIFDSVRFANDMGHTFFHVDSEEAHWN
                     SGKEYEGGNTGYRPMVKGGYFPVAPLDSLHDIRAEMCKTLEQVGIEVEVHHHEVANAG
                     QCEIGTRFNTLVQKADELLSMKYIIKNVAHRNGKTATFMPKPIVGDNGSGMHVHMSLA
                     KGGVNLFSGDGYGGLSQVALWYIGGIFKHARAINAFANSTTNSYKRLVPGYEAPVMLA
                     YSARNRSASCRIPYVTNPKARRIEIRFPDPMNSGYLIFAALMMAGLDGIKNQIDPGAP
                     SDKDLYDLPPEEEKNIPTVCHSLDQALEALDKDRDFLKAGGVFTDDFIDGYIALKMQE
                     VTRFRAATHPLEYQMYYTI"
     gene            3327..4181
                     /locus_tag="PK818_08460"
     CDS             3327..4181
                     /locus_tag="PK818_08460"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HQY82800.1"
                     /translation="MNDLLAALLLGIIEGITEFLPISSTGHLLIAERWLGHRSDLFNI
                     AIQAGAILAVVLIYWKRLWGMLSAFAGHDAPAPYDPLGVTATPAQSRDYAFKLLLAFA
                     VTAVGGLLVKKLGWELPDTVRPIAWALIIGAFWMVIAEQLAARRAARDGERTAISWTT
                     AILVGVAQVVAGVFPGTSRSAATIFVALLAGTTSRAAATEFAFVVGIPTMFAATAYEL
                     AAMLAGGGIGHEDWSALGVAFVASAITAFVAVKWLLHYIQSHRFTAFAVYRLALGIAL
                     LLWLPAGS"
     gene            4245..5138
                     /locus_tag="PK818_08465"
     CDS             4245..5138
                     /locus_tag="PK818_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4377 domain-containing protein"
                     /protein_id="HQY82801.1"
                     /translation="MKRLLLLALPALLAGCPKPPDGGAPTPASTPAASTETAKPEPVS
                     EQKPAAADTVAKVEFGSPGTIRAAGALPKFRWTLASAVDAKGQPVDALLVRPDRPLTL
                     EFKQGKLAIGNTCNRMLGAYSLRGNGMTVGELASTLMACPDPKLMALDREVGKRLQGK
                     LGLRLSKGDARQLELTTASGDVLVFTSEETAQTKYGGAGGRVFLEVAAQTAPCSHPLI
                     PNMQCLQVRELKYDEKGLKVGTPGPFGNFYSTIEGYTHQPGVRNVLRVDRYTVKMPPA
                     DAPSQVFVLDMVVESDASAKP"
     gene            complement(5142..5741)
                     /locus_tag="PK818_08470"
     CDS             complement(5142..5741)
                     /locus_tag="PK818_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HQY82802.1"
                     /translation="MRIHSDSFENGKPIPPEFAMGAPGGFGGNRNPHLAWDGAPAGTR
                     SFALLCIDTDAPTDGALVADAATPIPVEHPRGDFVHWVVADLPADLREIAAGSCSAGI
                     ATGGKPAGHDAGGMRGLNDYTGWFAGDAAMGGQYFGYDGPYPPPHDLRTHRYFFRLFA
                     LDVERIDLPPAFTAGDVFRAIRGHVLAEASTHGTYSLNG"
BASE COUNT          908 a         1913 c         2053 g         1035 t
ORIGIN      
        1 ccgccgtaga acagcgcaag gccgggcagg gtcatgaaga tcaccagcgc cgcggacacc
       61 agcacccaca cggtgtccgc cttgctgatg gtgggtgcgg gcgcggcgtc ctgcgcgaac
      121 aggttgccgc aggcgaacaa caggaacagc atcgccgcga tgcgtgcgcg ggattcgatg
      181 gaaggaagtc tcattgcgtc ctcgcgtggg ttagagcgca tcggcaccga gctcgccggt
      241 gcggatgcgg atgacgtggt cgagggcgac gacgaagatc ttgccgtcgc ccactttccc
      301 ggtcgcggcc gacttgctga tcgcgtcgag cgcagcgtcg aagtcgccgt cggcgaccgc
      361 gcattcgatc ttcagcttgg gcaggaaatc gacgacgtat tcggcgccgc ggtagagctc
      421 ggtatggccc ttctgccggc cgaagccctt gacctcggtg acggtgatgc cggacacccc
      481 gacttcggtg agcgcctcgc gcacctcgtc gagcttgaac gggcggatga tcgcggtgat
      541 cagtttcatc gttcgcagct cctcagaacg ccttggtgat ggccagggca acggtgtcgt
      601 cggcgacctt gttgccgaac gggttggtgt acagcgcgtc gtcggcgtcg gtgccggtgt
      661 aggccagccc gaccgagaag ccgttgtcga aggcgcgggt cacgcccagc ttccagtcgg
      721 tgtattcgaa ggcgtcgttg cccgcgatcc actgcctgcc ggcgtgcgcg ccgatggtcc
      781 agccttcggc caccgccagg ttcgcggtca ggtcgaggta gccgctgcca ccggaatcga
      841 catagccgaa caggtcggtg gtggagaccg agtacttcgc gcccaggctg atcttctcgc
      901 tggcggcgac cgtgatgccg gcatagacct caagggtgtc ggcgctgttg aagccggccg
      961 ggaagtcgcc gggataccag tagtacagcg cgcccacgtc gtagctgacg gcgtcgctga
     1021 acgtgccgcg atagccgccg tagacgtcga gctcgaggct gctggagagt ggcgccgcgg
     1081 tggtggagag atccgacagc cagctgatgc tgctgcccca ggcaccgatg taggcgccgc
     1141 tttcggcgct gaattcgacg cccccttgca gggcgggatc cgagttgctt tgcgagacgc
     1201 cgcggaacac gtagtcgctg gtcagcgaga cgttgccggt ggtgccggcc tgtgcatccg
     1261 cgatgccgaa cagggccagc agcgccatgg cgagacgcgt gcgggtgctg cgggtggtgg
     1321 tggtgcgcgt gtgcgtgcag ccgttggaaa ggatgcccgt cgtggtcgat gcatggaacg
     1381 gtgtagccac agtgtgagac gacatgacgc aggctcctca taagcggtgc cctccccggt
     1441 catttgtttc gtgctcgtgc cgtgcaggaa cgcgtcgtgc cctgccggct gcgatcgtcg
     1501 atcgcgtgct ccctccttgc gttggtgtcg cgcggcaccg ccgcgcatgg cccgccatgc
     1561 tcccttcccc tggccaaaag aggcgcggga ggcgcgcatc cgtcgatgcg cgctcccggc
     1621 ctgtccgatc ctcgccgggg agggagcgaa gcgaggatca gctcgcttcg tcccgatgca
     1681 tcgctgccgc gcagagcggc agcggccaca tcagatcgtg tagtacatct ggtattccaa
     1741 ggggtgcgtg gcggcgcgga agcgggtcac ttcctgcatc ttcagcgcga tgtagccgtc
     1801 gatgaagtcg tcggtgaaca cgccgccggc cttgaggaag tcgcggtcct tgtcgagcgc
     1861 ttccagcgcc tggtccaggc tgtggcagac ggtcgggatg ttcttctctt cttccggcgg
     1921 caggtcgtac aggtccttgt cgctcggcgc gcccgggtcg atctggttct tgatgccgtc
     1981 caggccggcc atcatcagcg cggcgaagat caggtagccg gagttcatcg gatccgggaa
     2041 gcggatctcg atgcggcgcg ccttcgggtt ggtcacgtac gggatgcggc acgacgccga
     2101 gcggttgcgc gccgaatacg ccagcatcac cggcgcctcg tagcccggca ccagccgctt
     2161 gtagctgttg gtggtggagt tggcgaaggc attgatcgcg cgggcatgct tgaagatgcc
     2221 gccgatgtac cacagcgcca cctgcgacag gccgccgtag ccgtcgccgc tgaacaggtt
     2281 gaccccgccc ttggccagcg acatgtgcac gtgcatgccg ctgccgttgt cgccgacgat
     2341 cggcttgggc atgaaggtcg cggtcttgcc gttgcggtgg gcgacgttct tgatgatgta
     2401 cttcatcgac agcagttcgt cggccttctg caccagggtg ttgaagcggg tgccgatctc
     2461 gcactggccg gcgttcgcca cctcgtggtg gtgcacttcc acctcgatcc cgacctgctc
     2521 cagcgtcttg cacatctcgg cgcggatgtc gtgcagcgaa tccagcggcg ccaccgggaa
     2581 gtagccgccc ttcaccatcg gccggtagcc ggtgttgccg ccctcgtatt ccttgccgga
     2641 attccagtgc gcttcctcgg aatcgacgtg gaagaaggtg tggcccatgt cgttggcgaa
     2701 gcggacgctg tcgaagatga agaactccgg ctccgggccg aagaacgcgg tgtcggcgat
     2761 gccgctggcc ttgaggaagg cctcggcgcg cttggccacg ccgcgcgggt cgcgcgagta
     2821 ggcctgcatg gtcgccgggt cgaggatgtc gcaggtcagg atcagggtcg ggtcggcggt
     2881 gaacgggtcg atgaccgcgg tcgatgcgtc cggcagcagg accatgtcgg actcgttgat
     2941 gcccttccag ccgctgatcg agctgccgtc gaacatcttg ccgtcctcga acaggctggc
     3001 gtcgacgatc gacttcggga aggtgacgtg gtgctgcacg ccacgcaggt cggtgaagcg
     3061 caggtcgacg aattcgacct tgtggtcgcg gatcagtttt tcgacgttct cgaacgacat
     3121 ggcaggactc cgggcaggat gtgggaagac gatgcaggga ttaatgcaag ctgcatgcca
     3181 agtctgcaag acattgattt tattggttat acgaaacttc gacgatcttc tttgcacgtg
     3241 tttggtgctg cctcgcgcag cgatggcacc aatctggtgc cagtgcgttg cgctatcctg
     3301 cgccactgcc cattcgccgg ttccgcatga acgacctgct cgccgcgctg ctgctcggca
     3361 tcatcgaggg gatcaccgaa ttcctgccga tttccagcac cggccacctg ctgatcgccg
     3421 aacgctggct gggccatcgc agcgacctgt tcaacatcgc gatccaggcc ggggcgatcc
     3481 tggcggtggt gctgatctac tggaagcggc tgtgggggat gctctccgcc ttcgccgggc
     3541 atgacgcccc ggcgccctac gacccgctgg gggtgaccgc aaccccggcg cagtcgcgcg
     3601 actacgcgtt caagctgctg ctcgcgttcg ccgtcaccgc tgtcggcggc ctgctggtca
     3661 agaaactggg ctgggagctg ccggacaccg tgcgcccgat cgcctgggcg ctgatcatcg
     3721 gcgcgttctg gatggtgatc gccgagcaac tggcggccag gcgcgcggcg cgcgacggcg
     3781 agcgcaccgc gatctcgtgg accaccgcga tcctggtcgg cgtcgcccag gtggtggccg
     3841 gcgtgttccc gggcacctcg cgcagcgcgg ccaccatctt cgtcgccctg ctcgccggca
     3901 ccaccagccg cgccgcggcc accgaattcg cgttcgtggt cggcatcccc accatgttcg
     3961 cggcgaccgc ctacgaactg gcggcgatgc tcgccggcgg cggcatcggc cacgaggact
     4021 ggagcgcgct gggggtggcc ttcgtcgcct ccgcgatcac cgccttcgtg gcggtgaaat
     4081 ggctgctgca ctacatccag agccaccgct tcaccgcgtt cgcggtgtac cgtctcgccc
     4141 tcggcatcgc cctgctgctg tggctccccg cgggcagctg agcgaaacgg cgcaaatgcc
     4201 gtgaacccgc cacacttccg atccacgctt gccggagaaa tgccatgaaa cgcttgctgc
     4261 tgctcgcctt gccggccctg ctcgccggtt gcccgaaacc accggacggc ggcgcgccaa
     4321 cgcctgcgtc aacgcctgcg gcgtccacgg aaacggccaa accggaaccg gtgagcgagc
     4381 aaaagcccgc ggccgcggac accgttgcca aggtcgagtt cggcagcccc gggaccatcc
     4441 gcgccgccgg tgccctgccc aagttccgct ggacgctggc cagcgcggtc gatgccaagg
     4501 gccagccggt cgatgccctg ctggtgcgtc ccgaccggcc gctcacgctg gagttcaagc
     4561 agggcaagct ggccatcggc aacacctgca accgcatgct tggcgcttac tcgctgcgcg
     4621 gcaacgggat gaccgtgggc gagctcgcct cgaccctgat ggcctgcccg gacccgaagc
     4681 tgatggcgct ggaccgggaa gtgggcaagc gcctgcaggg caagctgggg ctgcgcctgt
     4741 ccaagggcga tgcgcgccaa ctggagctga ccaccgcctc cggcgacgtg ctggtgttca
     4801 ccagcgagga aaccgcccag accaagtacg gcggcgccgg cgggcgcgtg ttcctggaag
     4861 tggcggcgca gaccgcgccg tgcagccatc cgctgatccc gaacatgcag tgcctgcagg
     4921 tgcgcgagct caagtacgac gagaagggcc tgaaggtcgg taccccggga ccgttcggca
     4981 atttctactc gacgatcgag ggttacaccc accaacccgg agtgcgcaac gtgctgcggg
     5041 tcgaccgcta caccgtcaag atgccgccgg cggatgcgcc atcgcaggtt ttcgtgctgg
     5101 acatggtggt ggagtcggac gcctccgcga agccgtaagg ttcagccgtt gaggctgtag
     5161 gtcccgtgcg tgctggcctc ggccagcacg tgcccgcgga tggcgcggaa cacgtcgccg
     5221 gcggtgaatg caggcggcaa gtcgatgcgt tccacgtcca gcgcgaacag cctgaagaag
     5281 tagcggtgcg tgcgcaggtc gtgcggcggc gggtacgggc cgtcgtagcc gaagtactgg
     5341 ccgcccatcg ccgcatcgcc ggcgaaccag ccggtgtagt cgttgaggcc gcgcatgccg
     5401 ccggcgtcgt ggcccgcggg cttgccgccg gtggcgatgc ccgcgctgca gctgccggcc
     5461 gcgatctcgc gcaggtcggc gggcaggtcc gccaccaccc agtgcacgaa atcgccgcgc
     5521 gggtgttcga ccgggatcgg ggtggcggca tcggccacca gcgcgccatc ggtgggggcg
     5581 tcggtgtcga tgcacagcag cgcgaaggag cgggtcccgg cgggtgcgcc gtcccaggcc
     5641 aggtgcggat tgcggttgcc gccgaagcca ccgggcgcgc ccatcgcgaa ttcgggcggg
     5701 atcggcttgc cgttctcgaa gctgtcgctg tggatgcgca tgggagtctc cgtccggatg
     5761 cccgtgattc tacgcagcgc cgggaatgga aacggggcca gagtgaagtt tcccgtccag
     5821 cattccggaa ccggtgaact tggtcgacgg gaaattcact ctggcccctt ttcccggacg
     5881 cacacacggt ctcgctagac cgctgcggc
//
LOCUS       DAORUK010000129         5891 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_16151_length_5891_cov_18.328307, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000129 DAORUK010000000
VERSION     DAORUK010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16151_length_5891_cov_18.328307"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PK818_08475"
     CDS             complement(<1..679)
                     /locus_tag="PK818_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005913582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HQY82803.1"
                     /translation="MRMDKLTSRFQQAIADAQSLAVGRDHTIIEPAHLLLALLDQQGG
                     GTRPLLAQAGVNVPVLRERLAEALDRLPKVSGQAGNVQAGNDLVRLLNVTDKLAQQRG
                     DAYIASELFLLAALEESGPAGKALKAAGGDKAKLDAAIDKLRGGEKVTDENAEDARQA
                     LEKYTIDMTARAESGKLDPVVGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIING"
     gene            820..1095
                     /locus_tag="PK818_08480"
     CDS             820..1095
                     /locus_tag="PK818_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HQY82804.1"
                     /translation="MTRGAWTLYLLRCRDGSYYAGITTDLDRRFREHAAGKGAKYTRA
                     HPPECVLGSRSYPDRAAASRAEHALKRQPKARKLAWLLAAADAASAG"
     gene            complement(1084..1833)
                     /gene="pgeF"
                     /locus_tag="PK818_08485"
     CDS             complement(1084..1833)
                     /gene="pgeF"
                     /locus_tag="PK818_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007973887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HQY82805.1"
                     /translation="MSAWLEADWPAPAGVRALTTTRHGLGVSRPPFDAFNLGTRCGDD
                     PAAVTENRRQLDAALRLPSPPRWLRQVHGIAVVPEPGFDEPEADAAVTAEAGVVLAIL
                     TADCLPVVFAARDGREIAAAHAGWRGLANGVLETTLAAMRTPAREVIAWLGPAAGPQA
                     YEIGEEVYDAFASRDPRALPAFVATRRGHWRVDLFALARLRLVDAGLTAAAIHGGGLC
                     TISDPQRFFSHRRDQRGGRIATLAWIVPSAG"
     gene            complement(1830..2813)
                     /gene="rluD"
                     /locus_tag="PK818_08490"
     CDS             complement(1830..2813)
                     /gene="rluD"
                     /locus_tag="PK818_08490"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HQY82806.1"
                     /translation="MNASAEPRHAIVPESAAGRRLDAVVAELFPEYSRSRLSAWIKSG
                     DVSVDGAPARGRDPVRGGEQVALNVVQETQTHAVAEDIPLDVLYEDEHVFVIDKPAGL
                     VVHPGAGNPAGTLVNALLFRDPALELLPRAGIVHRLDKDTSGVMVVARTLTAHTALVE
                     QLSNRQVHRQYLAVVVGALVSGGTANAAIDRHPRDRLRMAVREDGKDAVTHYRLRERF
                     RAHTALECRLETGRTHQIRVHMQHLKHPIVGDPLYGGSFKLPKGASEELIAALRAFKR
                     QALHAEVLEFMHPASGEPIRCSAPLPADMQQLLALLRADAAAHAEAERARR"
     gene            2925..3770
                     /locus_tag="PK818_08495"
     CDS             2925..3770
                     /locus_tag="PK818_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HQY82807.1"
                     /translation="MTLRSSLLRPIFVLALFALVAGSGCARMKGMFKDEDKNEGLPVS
                     QLYDKAHGDMEQGRWSSASETWGRLIAQYPYGPYTEQALMEQAYAEYKAGKHDDAVSS
                     IDRFIRTYPTHKNIAYLYYLRGLSNMARNAVFLSRAFKLDMSNRDLQAPQQAYNDFNT
                     VASRYPNSRYAADARQRMIFLRNEFARFELTTGLYYLRRGAWVAAADRATYLLETYPQ
                     SEYQDDAVAMLGESYTRLGNTALAADARRVLEQNDPQHPWLSGKWPRESRFYNRLNPF
                     AGDTH"
     gene            complement(3832..5472)
                     /locus_tag="PK818_08500"
     CDS             complement(3832..5472)
                     /locus_tag="PK818_08500"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011348332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HQY82808.1"
                     /translation="MAASTLRIALAQFDFPVGDVAGNRDRIAALIAGARDEYDADIVL
                     FPELALSGYPPEDLLLRPAFLRECEAALQAIAAGVHGIVAVVGWPQAAGAVVYNAASV
                     LRDGAVEQTYRKRELPNYAVFDERRYFDVDPDRDDCVFEVKGTPVGLVICEDLWFAEP
                     VASTVAKGAQLLLVPNASPFERDKHAQRDALLAQRVAETGAAIAYLNLVGGQDALVFD
                     GASVVADGDGSVHPAAAAFTDQWLVVDFDPSSRRFTPLQWMEDGDESRDALAWRAVVR
                     GTRDYCRKNGFDKAWLGLSGGIDSALVLAIAADALGAGNVTAVRMPSRYTAGLSNDLA
                     QEQCDAMGVELVTLPIEKPFQGYLDALGPVFAGKAVDVTEENLQSRVRGALMMALSNK
                     FGGLLLTTGNKSEYAVGYATIYGDMCGGYAPLKDLYKTEVFALARWRNTVGDRVVPQA
                     VIDRPPSAELRENQKDQDSLPPYDVLDAILLRHVDQEQSRAEIVAAGFDAATVDKVLR
                     LVRIGEWKRHQAAPGPKVSRRAFGRERRYPVSSGWREN"
     gene            complement(5536..>5891)
                     /locus_tag="PK818_08505"
     CDS             complement(5536..>5891)
                     /locus_tag="PK818_08505"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418610.1"
                     /note="Catalyzes the only substrate-level phosphorylation
                     in the TCA cycle; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HQY82809.1"
                     /translation="TCIGIGGDPINGTNFIDALKLFQDDPQTEGIIMVGEIGGSAEEE
                     AAEFIAKHVTKPVVGFIAGASAPKGKRMGHAGAIASGGTGTAEGKFAALEAAGVTTVK
                     SPGDLGAAIAARLAK"
BASE COUNT          868 a         2162 c         1975 g          886 t
ORIGIN      
        1 cgccgttgat gatgcgctgg gcgaggcctt ccacgatcgc ggtcttgccc acgcccggct
       61 cgccgatcag gaccgggttg ttcttggtgc gccgctgcag cacctggatg gtgcggcgga
      121 tttcctcgtc gcggcccacc accggatcga gcttgccgct ctccgcgcgg gcggtcatgt
      181 cgatggtgta tttctccagc gcctggcgcg catcttccgc gttctcgtcg gtcactttct
      241 cgcctccgcg cagcttgtcg atcgccgcat ccagtttcgc cttgtcgccg ccagccgcct
      301 tcagtgcctt gccggccggg ccggactcct ccagtgccgc cagcaggaac agctcgctgg
      361 cgatgtaggc atcgccgcgc tgctgggcca gcttgtcggt gacgttgagc aggcggacca
      421 ggtcgttgcc ggcctgcacg ttgccggcct ggccgctgac ctttggcagc cggtcgagcg
      481 cctcggcgag ccgctcgcgc agcaccggca cgttgacgcc ggcctgcgcc agcagcgggc
      541 gggtgccgcc gccctgctgg tccagcagcg ccagcagcag gtgcgcgggt tcgatgatgg
      601 tgtggtcgcg gcccacggcc agcgattgcg cgtcggcaat cgcctgctgg aagcgcgagg
      661 tgagtttgtc catgcgcatg ggggaattcc tgcaggaagg ggccagcggg tggccgatac
      721 ttcccacatg cggttgccgc gccgtgattc aagtcggcac gcgcaccgcg ttcgcggccg
      781 ggttatggtc gccgcaccga cccgccagcc ggatcctgga tgacccgcgg agcctggacc
      841 ctttacctgc tgcgctgccg cgacggcagc tactacgccg ggatcaccac cgaccttgat
      901 cgccgcttcc gcgagcacgc cgccggcaag ggcgcgaagt acacgcgcgc gcatccgccc
      961 gagtgcgtgc tgggcagccg cagctacccg gaccgggccg ccgcatcgcg cgcggagcac
     1021 gccctcaagc gccagccgaa ggcgcgcaag ctggcctggc tgctggctgc cgccgacgcc
     1081 gcttcagccg gctgagggca cgatccaggc cagggtcgcg atccggccgc cgcgctggtc
     1141 gcgacggtgc gaaaagaagc gctgcgggtc ggagatcgtg cacaggccac cgccgtggat
     1201 cgcggccgcg gtgaggcccg catccaccag ccgcaagcgc gccagcgcga acaggtcgac
     1261 ccgccagtgc ccgcggcggg tggcgacgaa cgccggcaac gcacgcggat cgcgcgaggc
     1321 gaatgcgtcg tagacctcct cgccgatctc gtaggcctgc ggcccggcgg ccgggccaag
     1381 ccaggcgatg acctcgcgcg cgggcgtgcg catcgccgcc agcgtcgttt ccagcacgcc
     1441 attcgccagc ccgcgccaac cagcgtgggc ggcggcgatc tcgcggccat cccgcgcggc
     1501 gaacaccacc ggcaggcagt cggcggtgag gatcgccagc accacgcccg cctcggcggt
     1561 caccgcggca tccgcctcgg gctcgtcgaa gcccggctcc gggaccaccg cgatgccatg
     1621 cacctgccgc aaccaacgtg gcggcgaagg cagccgcaac gcagcgtcga gctggcgccg
     1681 gttttcggtg accgccgcgg ggtcgtcgcc gcagcgcgtg ccgaggttga acgcatcgaa
     1741 ggggggccgc gagaccccga ggccatgccg ggtcgtggtc aacgcccgca cccccgctgg
     1801 cgccggccag tcggcctcca gccacgcgct catcggcgcg cgcgctccgc ctccgcgtgc
     1861 gctgcggcat ccgcgcgcag cagcgccagc aattgctgca tgtccgccgg cagcggggcg
     1921 ctgcaacgga tcggctcgcc gctggcggga tgcatgaatt ccaggacttc cgcatgcagg
     1981 gcctggcgtt tgaacgcgcg cagggcggcg atcagctcct ccgacgcccc cttcggcagc
     2041 ttgaacgaac cgccgtacag cggatcgccg acgatcgggt gcttgaggtg ctgcatgtgc
     2101 acccggatct ggtgggtgcg cccggtttcc agccggcatt ccagcgcggt gtgggcgcgg
     2161 aagcgttcgc gcaggcggta gtgggtgacg gcgtccttgc catcctcgcg cacggccatg
     2221 cgcaggcggt cgcgcgggtg gcggtcgatc gcggcattcg cggtgccgcc ggagaccagc
     2281 gcgccgacga ccaccgccag gtactggcga tgcacctgcc ggttggacag ctgctccacc
     2341 agcgcggtat gcgcggtcag ggtgcgcgcc accaccatca ccccgctggt gtccttgtcc
     2401 agccgatgca cgatgccggc gcgcggcagc aactcgagcg cggggtcgcg gaacagcagg
     2461 gcattgacca gggtgccggc cggattgccc gcgcccggat gcaccaccag ccctgccggc
     2521 ttgtcgatca cgaacacgtg ttcgtcctcg taaagcacgt ccagcgggat gtcctcggcg
     2581 accgcatggg tctgggtctc ctgcaccacg ttcagcgcaa cctgctcgcc accgcggacg
     2641 ggatcccggc cccgcgcggg cgcgccgtcc acgctcacgt cgcccgactt gatccaggcg
     2701 gacaaccgcg agcgcgagta ctcggggaac aattcggcca ccaccgcgtc cagccggcgg
     2761 ccggcggcgg attcggggac gatggcgtgg cgcggttcgg cactggcatt cataggcggg
     2821 tccggacccg gcggcgcggc aagcgccggc gccgggttgg gcttggttca accatgggac
     2881 tgttattatc gttgcttcat gcccccggcg cccgttcgat gcccatgacc ctgcgttcat
     2941 ccctgctgcg cccgatcttc gtgcttgcgc ttttcgccct tgtcgccggc agcggttgcg
     3001 cccggatgaa gggcatgttc aaggacgagg acaagaacga aggcctgccg gtcagccagc
     3061 tctacgacaa ggcccatggc gacatggagc agggccgctg gagcagcgcc agcgaaacct
     3121 ggggccgcct gatcgcgcag tacccgtatg gcccgtacac cgaacaggcg ctgatggaac
     3181 aggcctatgc cgagtacaag gcgggcaagc acgacgacgc ggtctccagc atcgaccgct
     3241 tcatccgcac ctacccgacc cacaagaaca tcgcctacct gtattacctg cgcggcctgt
     3301 ccaacatggc ccgcaacgcg gtgttcctgt cccgcgcgtt caagctggac atgagcaacc
     3361 gcgacctgca ggcaccgcag caggcctaca acgacttcaa taccgtcgcg tcgcgctacc
     3421 cgaacagccg ctacgccgcc gacgcgcgcc agcgcatgat cttcctgcgc aacgaattcg
     3481 cccgcttcga gctcaccacc ggcctgtact acctgcgccg cggcgcctgg gtggccgccg
     3541 ccgaccgtgc cacctacctg ctggaaacct acccgcagag cgaataccag gacgacgcgg
     3601 tggccatgct gggcgagtcc tacacccgcc tcggcaacac cgcgctggcc gcggacgccc
     3661 gccgcgtgct ggagcagaac gacccgcagc atccgtggct gtccgggaag tggccgcgcg
     3721 aaagccgctt ctacaaccgc ctgaatccgt tcgccggcga cacccactga ggtcgccgcc
     3781 cgaaaggggg tccggttcac ttcccgatgc ggacgtgaac cggaccccct ttcagttttc
     3841 gcgccagccg ctgctcaccg ggtagcgccg ctcgcggccg aacgcgcgcc ggctgacctt
     3901 cggccccggc gcggcctggt gccgcttcca ctcgccgatc cgcaccagcc gcagcacctt
     3961 gtccacggtg gcggcatcga agccggcggc cacgatttcc gcgcgcgact gttcctggtc
     4021 gacatgccgc agcaggatcg cgtccagcac gtcgtagggt ggcagcgagt cctggtcctt
     4081 ctggttttcg cgcagctccg cagatggcgg ccggtcgatc accgcctgcg ggaccacccg
     4141 gtcgcccacc gtgttgcgcc agcgcgccag cgcgaacacc tcggtcttgt agagatcctt
     4201 gagcggcgca tagccgccgc acatgtcgcc gtagatggtg gcgtagccga ccgcgtactc
     4261 gctcttgttg ccggtggtca gcagcagtcc gccgaacttg ttggacagcg ccatcatcaa
     4321 cgcaccgcgc acgcgcgact gcaggttttc ctcggtgaca tccaccgcct tgccggcgaa
     4381 caccggcccc agcgcatcca gatagccctg gaacggcttc tcgatgggca gcgtgaccag
     4441 ctccacgccc atcgcgtcgc attgttcctg cgccaggtcg ttggacaggc cggcggtgta
     4501 gcgcgacggc atgcggaccg cggtgacgtt gcccgcgccc agcgcatcgg cggcgatcgc
     4561 cagcaccagc gccgaatcga tgccgccgga cagccccagc caggccttgt cgaagccgtt
     4621 cttgcggcag tagtcgcggg tgccgcgcac caccgcccgc caggccagcg catcgcggct
     4681 ctcgtcgcca tcctccatcc actgcagcgg ggtgaagcgg cgcgatgacg gatcgaaatc
     4741 caccaccagc cactggtcgg tgaaggccgc cgcagcgggg tgcacgctgc cgtcgccgtc
     4801 ggccaccacc gacgcgccgt cgaacaccag cgcgtcctgg ccaccgacca ggttgaggta
     4861 ggcgatcgcc gccccggttt ccgcaacccg ctgcgccagc agggcatcgc gctgcgcgtg
     4921 cttgtcgcgc tcgaacggcg aggcgttcgg caccagcagc agttgcgcgc ccttcgccac
     4981 cgtggaggca accggctcgg cgaaccacag gtcctcgcag atcaccaggc caaccggcgt
     5041 gcccttgacc tcgaacacgc agtcgtcccg gtccgggtcc acgtcgaaat aacggcgttc
     5101 gtcgaacacc gcgtagttcg gcagctcgcg cttgcggtag gtctgctcga cggcgccgtc
     5161 gcgcagcacg ctggccgcgt tgtagaccac cgcccccgcc gcctgcggcc agccgaccac
     5221 cgcgacgatg ccatgcacgc ccgcggcgat cgcctgcaac gcggcctcgc attcgcgcag
     5281 gaacgccggc cgcagcagca ggtcctccgg tggatagccg ctcagcgcga gctccgggaa
     5341 cagcacgatg tcggcgtcgt actcgtcgcg cgccccggcg atcagggcgg cgatgcgatc
     5401 gcggttgccg gccacgtcgc cgaccgggaa atcgaactgc gccagcgcga tgcggagcgt
     5461 ggatgcggcc atcgaacctc ctggaaaacg aaaggccgca cgcggcggcc ttccggtctt
     5521 gcgttgcagc gaagtctact tcgccaggcg tgccgcgatc gccgcgccga ggtcgccggg
     5581 gctcttcacg gtggtcacgc cggccgcttc cagcgcggcg aacttgcctt ccgcggtacc
     5641 ggtgccgccg gaggcgatcg caccggcgtg gcccatgcgc ttgcccttcg gcgcggacgc
     5701 gccggcgatg aagccgacca ccggcttggt gacgtgcttg gcgatgaact cggccgcttc
     5761 ctcttcggcg gaaccgccga tctcgccgac catgatgatg ccttcggtct gcggatcgtc
     5821 ctggaacagc ttcagggcgt cgatgaagtt ggtgccgttg atcgggtcgc cgccgatgcc
     5881 gatgcaggtc g
//
LOCUS       DAORUK010000130         5816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_16452_length_5816_cov_22.046173, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000130 DAORUK010000000
VERSION     DAORUK010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16452_length_5816_cov_22.046173"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3165)
                     /locus_tag="PK818_08510"
     CDS             complement(<1..3165)
                     /locus_tag="PK818_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY82810.1"
                     /translation="MKFTDLFIRKPVLALVVSLFILLFGLRAFSELNVRQYPEMRNAV
                     VNVSTTYFGADADLIQGFITTPLEREIASAEGIDYLTSTSAAGISTIQAYIQLDRDPN
                     EALTQISAKVNKMRGQLPAESEAPVIDLQQGQQIAAMYVSLASETLDNNQITDYITRV
                     IQPKLVTIDGVQRADILGAGTFAMRIWLKPDRMTAYKVTASDVSTALSSNNVLAAVGS
                     TKGQMVAIDMTARTDLRNAEEFRKLVVREEGGAIVRLGDVADVVLGSESYGTSVRING
                     EAATFMGIFVAPDANSLDVIREVRKVWDAEIVPQLPQGIKATIPYDSTEAIQDAINEV
                     ISTIVEALVIVIVVIFLFLGSLRSVLIPAVTIPLSLVGALFLMLLMGFTINLLTLLAM
                     VLAIGIVVDDAIIVLENIHRHIEEGMTPFDASIRGARELAWPVVAMTTTLVAVYLPIG
                     FQGGLTGVLFTEFAFTLAGAVLLSGVIALTLTPMMSSRILTSHDPEHPKSRLENWLDE
                     HFERLRLAYQRRLHGAMETRSVVAAFGLIVLVSCAFLYTGAPKEQAPLEDEGFIFSIA
                     SADPTATLDYVERYTGEITRIAKGVPEVQDYFLFNGGFGGSGGGASPMAMAGFVLKPW
                     SERTRSTNQVLQQELQPKMAQVTGLNVFALVPPSLPSAGGDGGGGEFVIGGVGGLGQL
                     AELADQVLLKAMASKRFIFLDKDLKIDKPQIEVNVDRDKAATLGIDMRTLAADMAAML
                     SGGYSNRFAMQNRSYLVIPQVQRSDRLNASALENYYTRTRDGQLIPLSTIVTLKDSTQ
                     PQSLKRFQQLNAVSITFAARPGVSKGEALAILEQAAKDVLPQGYSVDYAGESRQFKQE
                     GQAMLMTLALALVVIFLVLSAQFESFRDALIMLLTVPMAICGALLALNVLALVANVLQ
                     FAGIEAFPGMSINIYTQVGLVTLVGVISKHGILIVEFANKLQSEQGLSKREAIEEATG
                     IRLRPVLMTTAALVFAMIPLLVASGPGAASRFSMGVVIASGMTIGTLFTLFVLPAFYL
                     YLARDHEHDADHEKVIEGDASSLPP"
     gene            complement(3167..4357)
                     /locus_tag="PK818_08515"
     CDS             complement(3167..4357)
                     /locus_tag="PK818_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY82811.1"
                     /translation="MSKPSPKRPPNPTLRWVLMLAGVIVVFGGVFAIKAFFAKQTDNF
                     FDNMPQPAVAVSSAVAKVQRWSDDGEAVGTFVAVNGTDVTTEAGGVVRSIAFEAGQPV
                     AAGTVLLRLNTANEQATLDALEASARLARVQANRWQQLGKEKLVSLDDVQSRVTAAAT
                     AQAQVDAQRALIAQKTIRAPFSGVLGLRKVNLGQYIAPGAAIVSLQQLDPIHLDFSLP
                     EQRMGKLVEGSAVRAGIDALPGQVFEGQVTAIEPQVDPATRNFKAQATFRNPDGALRP
                     GSFASITFAQGGERDVLVIPQTAVSFNPYGNAVFVIGKQKRKPGETDMQGQPLSGDKL
                     VVTQRFVKTGATRGDLVVVSEGLKAGEQVVTSGLLKLRNDAEVTINDSIQPAADARPT
                     PENR"
     gene            complement(4551..4943)
                     /locus_tag="PK818_08520"
     CDS             complement(4551..4943)
                     /locus_tag="PK818_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HQY82812.1"
                     /translation="MYQFNEQFTKSTAQFADVAANVNRLALENVQNAFGLQLAAIEEN
                     ATAAFAFIGELTNVRDFDGLKAVLPKGAQIARTSTERSLGTAQEVFANTIKTNEAIGA
                     LAKGQFEKATAQAQAEVEKVTKAASKAK"
     gene            complement(5148..5642)
                     /locus_tag="PK818_08525"
                     /pseudo
     CDS             complement(5148..5642)
                     /locus_tag="PK818_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
BASE COUNT          898 a         1909 c         1981 g         1028 t
ORIGIN      
        1 gggtggcagc gatgacgcat cgccttcgat caccttttcg tggtcggcat cgtgctcatg
       61 gtcgcgtgcc aggtacaggt agaacgccgg cagcacgaac agggtgaaca gggtaccgat
      121 ggtcatgccg gaggcgatca ccacgcccat cgagaagcgc gacgcggcac ccgggccgct
      181 ggcgaccagc agcgggatca tcgcgaacac cagcgcggcg gtggtcatca gcaccgggcg
      241 caggcggatg ccggtggctt cctcgatcgc ctcgcgcttg gacaggcctt gctcgctctg
      301 cagcttgttg gcgaactcca cgatcaggat gccgtgcttg gagatcacgc cgaccagcgt
      361 caccaggccg acctgggtgt agatgttgat gctcatgccg gggaaggctt cgatcccggc
      421 gaactgcagc acgttggcca ccagcgccag cacgttcagc gccagcagcg cgccgcagat
      481 cgccatcggc accgtcagca gcatgatcag cgcgtcgcgg aaactctcga actgcgccga
      541 cagcaccagg aagatcacca ccagcgccag cgccagggtc atcagcatgg cctggccttc
      601 ctgcttgaac tgccgcgact cgccggcgta gtccacgctg tagccctgcg gcaggacgtc
      661 cttcgccgcc tgttccagga tcgccagtgc ctcgcccttg ctcacgccgg ggcgcgcggc
      721 gaaggtgatc gacaccgcgt tgagctgctg gaagcgcttc agcgactgcg gctgggtgct
      781 gtccttcagg gtcacgatgg tggacagcgg gatcagctgg ccgtcgcggg tgcgggtgta
      841 gtaattctcc agcgccgagg cgttcaggcg gtcgctgcgc tgcacctgcg ggatcaccag
      901 gtacgagcgg ttctgcatcg cgaagcggtt ggaatagccg ccggacagca tcgccgccat
      961 gtccgcggcc agcgtgcgca tgtcgatgcc gagcgtcgcc gccttgtcgc ggtccacgtt
     1021 gacctcgatc tgcggcttgt cgatcttcag gtccttgtcg aggaagatga agcgcttgct
     1081 tgccatcgcc ttcagcagga cctggtcggc cagttccgcc agctggccga gcccgccaac
     1141 cccgccgatg acgaattcgc cgccaccgcc gtcgccgccg gcgctgggca gcgagggcgg
     1201 caccagcgcg aacacgttca ggccggtcac ctgcgccatc ttcggctgca gctcctgctg
     1261 cagcacctgg ttggtcgagc gcgtgcgctc gctccacggc ttgagcacga agccggccat
     1321 cgccatcggg ctggcgccac cgccgctgcc gccgaagccg ccgttgaaca ggaagtagtc
     1381 ctgcacctcg ggcacgccct tggcgatccg ggtgatttcc ccggtgtagc gctccacgta
     1441 gtcgagggtg gcggtggggt cggcgctggc gatcgagaag atgaagccct cgtcttccag
     1501 cggcgcctgt tccttcggcg ccccggtgta caggaacgcg cagctgacca gcacgatcag
     1561 gccgaacgcg gcgaccaccg aacgggtttc catcgccccg tgcaggcgcc gctggtaggc
     1621 caggcgcagg cgttcgaaat gctcgtccag ccagttctcg aggcggctct tggggtgctc
     1681 ggggtcgtgc gaggtgagga tgcgcgagct catcatcggc gtcagcgtca gcgcgatcac
     1741 ccccgacagc agcaccgcgc cggccagggt gaacgcgaat tcggtgaaca gcaccccggt
     1801 cagcccgccc tggaagccga tcggcaggta caccgcgacc agggtggtgg tcatcgccac
     1861 caccggccac gccagttcgc gcgcgccgcg gatcgaggcg tcgaacggcg tcatgccttc
     1921 ctcgatgtgc cggtggatgt tctccagcac gatgatcgcg tcgtcgacca cgatcccgat
     1981 cgccagcacc atcgccagca gggtcagcag gttgatggtg aagcccatca gcagcatcag
     2041 gaacagcgcg ccgaccagcg acagcgggat ggtcaccgcc gggatcagca cgctgcgcag
     2101 cgacccgagg aacaggaaga tcaccacgat cacgatgacc agcgcttcca cgatggtgct
     2161 gatgacctcg ttgatcgcgt cctggatcgc ctcggtgctg tcgtagggga tggtcgcctt
     2221 gatgccctgc ggcagctgcg ggacgatttc ggcatcccac accttgcgca cctcgcggat
     2281 cacgtccagc gagttggcgt cgggggcgac gaagatgccc atgaaggtgg ccgcttcgcc
     2341 gttgatgcgc accgatgtgc cgtagctctc cgagcccagc accacgtcgg ccacgtcgcc
     2401 caggcggacg atggcgccgc cttcctcgcg caccaccagc ttgcggaatt cctcggcgtt
     2461 gcgcaggtcg gtgcgcgcgg tcatgtcgat cgcgaccatc tggcccttgg tcgagccgac
     2521 cgcggccagc acgttgttcg acgacagcgc ggtggagacg tcgctggcgg tgaccttgta
     2581 cgcggtcatc cggtccggct tcagccagat gcgcatggcg aaggtgccgg cgccgaggat
     2641 gtccgcgcgc tgcacgccgt cgatggtgac cagcttgggc tggatcacgc gggtgatgta
     2701 gtcggtgatc tggttgttgt cgagcgtctc gctggccagc gacacgtaca tggccgcgat
     2761 ctgctggccc tgctgcaggt cgatcaccgg cgcttccgac tcggccggca gctgcccgcg
     2821 catcttgttg accttggccg agatctgggt gagcgcctcg ttcgggtcgc ggtccagctg
     2881 gatgtaggcc tggatcgtgc tgatgccggc ggcgctggtc gaggtcaggt agtcgatgcc
     2941 ctcggcgctg gcgatctcgc gctccagcgg ggtggtgatg aagccctgga tcaggtcggc
     3001 gtcggcgccg aagtaggtgg tgctgacgtt gaccaccgcg ttgcgcattt ccgggtactg
     3061 gcgcacgttc agttcgctga aggcgcgcag cccgaacagc aggatgaaca ggctgacgac
     3121 cagcgccagc accggtttcc ggatgaagag atcggtgaat ttcatctcag cggttttccg
     3181 gcgtgggccg cgcgtcggcg gccggctgga tgctgtcgtt gatcgtcacc tcggcgtcgt
     3241 tgcgcagctt cagcaggccg ctggtcacca cctgctcgcc ggccttgaga ccctcgctga
     3301 ccaccaccag gtcgccgcgg gtcgcgccgg tcttgacgaa gcgctgggtc accaccagct
     3361 tgtcgccgct cagcggctgg ccctgcatgt cggtttcacc gggtttgcgc ttctgcttgc
     3421 cgatcacgaa caccgcattg ccgtaggggt tgaagctgac cgcagtctgc gggatcacca
     3481 gcacgtcgcg ttcgccaccc tgggcgaagg tgatgctggc gaagctgccc gggcgcagcg
     3541 cgccatccgg gttccggaag gtggcctggg ccttgaaatt gcgggttgcg gggtcgacct
     3601 ggggctcgat cgcggtgacc tggccttcga acacctggcc gggcaaggcg tcgatgccgg
     3661 cgcggaccgc gctgccttcg accagcttgc ccatccgctg ttccggcagg ctgaagtcga
     3721 ggtggatcgg atccagttgc tgcaggctga cgatggccgc gccgggcgcg atgtactggc
     3781 cgaggttgac cttgcgcagg ccaagcacgc cgctgaacgg cgcgcggatg gtcttctgcg
     3841 cgatcagcgc gcgctgggca tcgacctgcg cctgcgcggt ggcggcggcg gtgacccggc
     3901 tttgcacgtc gtccagcgag accagctttt ccttgcccag ttgctgccag cggttggcct
     3961 gcacgcgggc aagcctggcg gatgcttcca gcgcatccag cgtggcctgt tcgttggcgg
     4021 tgttgaggcg cagcagcacg gtgccggcgg caaccggttg cccggcttcg aacgcgatcg
     4081 aacgcaccac gccacccgct tcggtggtga cgtcggtgcc gttgaccgcg acgaaggtgc
     4141 cgaccgcttc gccgtcatcg ctccagcgct gcaccttcgc gaccgcggaa ctgaccgcca
     4201 ccgccggctg cggcatgttg tcgaagaagt tgtcggtctg tttggcgaag aacgccttga
     4261 tcgcgaacac cccgccgaac acgacgatga ccccggccag catcagcacc cagcgcaagg
     4321 tgggattcgg cgggcgcttc ggggacggtt tcgacatggt ggcctctgct actgcgggat
     4381 gttattgaac cgactggtcc aatattctgc gcccgaaagc gagtgggacg caatcgtgac
     4441 cgacggcagg gtatgtcctg cagccagcaa cgaaaaagcc ccgcagcttg cgctgcgggg
     4501 cctgggttcg cggggcggat ggagggggag cgttccgcgc ccgcgggggg tcacttggcc
     4561 ttggacgcgg ccttggtcac cttttccact tcggcctggg cctgggcggt ggccttctcg
     4621 aactggccct tggccagcgc gccgatggct tcgttggtct tgatggtgtt ggcgaacact
     4681 tcctgcgcgg tgccgaggct gcgctcggtg ctggtgcggg cgatctgggc acccttcggc
     4741 agcacggcct tcaggccgtc gaaatcgcgc acgttggtca gctcgccgat gaaggcgaac
     4801 gcggcggtgg cgttctcttc gatcgcggcc agctgcaggc cgaacgcgtt ctggacattc
     4861 tccagggcga ggcggttgac gttggcggcg acgtcggcga actgggcggt ggacttggtg
     4921 aactgctcgt tgaactggta catggcgggt ctcctgcttg ttccggcagg ggttgccggg
     4981 tttgtgcggt gcagcatatg cgccgctatg ttgcgatgca acatcgaacc gacgaacggt
     5041 ctcccgccat tcagcttcaa tcaaatcaac cgcttaggcc ggcgccgggc tcagccgcgg
     5101 cggcgcaggc tggcgtgcat cggcggcaac agcccttcgg ctggcggtgg caccgccacc
     5161 acccccgcga ccgcccgtgc gaacggcgcc aggtggtcca gcgcgcgcgc gtacacggtg
     5221 cgcttgaagg tcaccacgtg ctgcatcggg taccagaaat cgacccagcg ccagccgtcg
     5281 aattccggtt tgtcggtcag gtccaggcgc aggtcggcgt cggcaccctt gaaccgaagc
     5341 aggaaccaga cctgcttctg gccgatgcag accagccggt catggcggcg caccgcccgc
     5401 ggcggcagcc ggtaacgcag ccagcccggg gtggtaccca gcacgtccac gtgctccggg
     5461 agcaggccgg tctcttcccg cagttcgcgg tacatcgctt ccagcggggt ctcgtcggtg
     5521 ttcatcccgc cctgcgggaa ttgccaaccg tcccggtgca cgcggcgtgc ccagaacacc
     5581 cgaccatcct cgtgcatcag cacgatgccc acgttggggc ggaagccgtc cggatcgatc
     5641 acgatcggac tccggcaaat tcactggttc cgactctgcc accaccccgc acatgcggca
     5701 agcatggcct gcatggattg acgacagcgt gcccgacgtg aataattcgc ggttctcagc
     5761 gtggctatgt agctcagccg gttagagcac agcactcata atgctggggt cggtgg
//
LOCUS       DAORUK010000131         5761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_16677_length_5761_cov_20.020505, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000131 DAORUK010000000
VERSION     DAORUK010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16677_length_5761_cov_20.020505"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3878)
                     /locus_tag="PK818_08530"
     CDS             complement(<1..3878)
                     /locus_tag="PK818_08530"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HQY82813.1"
                     /translation="MSGHERDHRVHRHGLAPLPADASGEQREARIAELRQLRRTRRRT
                     LALRGGIATLAMAAGVALLLYWLLMTIGGRDLLLRELVARLPAGTELTWDTAEGPASG
                     PMVLRGVHFSVPRQRDPDCVATTRTRCAMGRIVLDAEQVTLDPSLRPLLGRTLRLDTL
                     DIAGATLDLPRSDKPFELPRWPEVLPQVELPLALRAGAIRIDGLKVVQEGEPLIDIRS
                     ARGGLDAAPGSLHIERLAVDSDRGRFTLHGDYIPRRNFRSDLRATAVLPAPAGRTAPR
                     FGLIARGDLSKLDVAIGGRAPAPLRAVLTLRGDADRPRWQLRANSDALDIGLLGGSGE
                     ASTPLAFNLGADGIGGSANLRGTLKQGDFAATLQPSKLRLEDRVLQLQPLVLDVFGGR
                     VNANGSADLRDPDDASLKFAVNARGLRWQGEGGDTMVAGDADFGIAGTPARWAVKGQA
                     RLQRGQERATVDLAGRGFRERIQVDVLRARMPQGRLDASGEFAWSPRLSWTAKATLAG
                     FDPGYFAPDWPGAVDGVVLSSGVRRDNGSLLADAEAGNLGGTLRGRALGGRGTLNIDG
                     DNYAGDVALSLGGSRIDARAKIAAGIDIEANLSPLQLDDLLPDGRGSMRGMFTLRGAR
                     TAPDVDIDLRGEGVAFGDYRAEQFIAKGRLPWRTGNGMLAVEAQGLQLGLPLSSLRAG
                     LRGSVERLQFDADAQSEFGALALRGDANKQGTRWQGALASLQFDPAKGAAWTLQQPAR
                     WSWDGSSGALSRACLLANTGGTLCADADWPRRGVELQGDALPLALLVPYLPEREDSRP
                     WVFNGEAGLRVRIVPAANAWRGTASITSAAGGIRNRPRARRDLAGYRDLQLDATFDPQ
                     AIGISLGTVFNDDGRIDARISTGWDGYAPLAGEIKVSTDELTWMELFSPDIVAPTGRL
                     DVDLRLGGTRAAPAIGGEGHLQGFASELPALGIAARDGDLRLQAQADGNARIVGRVRS
                     GDGVLDVDGSLGWQAQDTPLVLNLRGTNVLIAETRQLRAVANPDVVVRYRAGEPLQVS
                     GSVTLPEADIHLERLDEGVSRSDDVVVLDPVDPERTRANPLDLDLTLVMGEQVAINGF
                     GLVGTLGGRLRVRALPGREVRGTGALDVAGRYTAYAQRLQITRGRLLWSDTPVGDPLL
                     DIRAERQVGEVTAGIRVEGRASAPRATVYSNPAKSESEALAYLTLGRPLSSLTGDEAR
                     QIGTARAALAAGTALVASELGQRLGLDDAGVVGSRALGADVLSIGKYLSPKLYVGYGV
                     SLLGTGQVLMLKYLLRKGFDIQIESSTVENR"
     gene            complement(3875..5728)
                     /locus_tag="PK818_08535"
     CDS             complement(3875..5728)
                     /locus_tag="PK818_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HQY82814.1"
                     /translation="MPPRPARLLIALALAGVALPASAITVTRIEVSGLADERMAQNVR
                     LSLSLNDELGKDLSARRLSYLLREAEAETREALEPFGYYTPTIVIQRSDRQRPEGADA
                     EAGTSASVRTDAEGDGERSVSVAISIAPGNQVRVRAWNVGIDGAGADDPHVAAALAGF
                     APRSGEVLDHAVYEASKSKVSRMLAGRGYFDADFSAHRVAVTRAEHAADIDLRWRSGA
                     RHALGSVDFGQEPQAVIDDSLLRKLVGWEPGEPWDDAELERLRRSLVALDYFGLVEVL
                     PQPAEKTGDVVPVQVHLTPAPRSIYSIGASYGTQSGAGVSLGFERRYLNARGHKALAQ
                     IDYASKRKLATVQYRVPAFAWLDGWYTASLQAADEQTDSLDNRRLEFVASRSGQYNQH
                     LNLVASAHVLRERWAYFTITKPTQSYEYASFAFPSLRAEYIDVDDRLAPRRGTGGTLE
                     LRGGNGGADGTTGFVQLHADAHWFHGMQDDSRLLVRGEAGHTFTNELLELPPSLRYYA
                     GGDRSIRGYGWREVGPRIIDSAGNAYAVGASNVVTASIEYERYFKGPWGAAVFVDSGS
                     AFDGSKPDLHTGVGFGVRWRSPVGPVRVDIARGLKSPDSPFTLHLNIGADL"
BASE COUNT          782 a         2081 c         2088 g          810 t
ORIGIN      
        1 cggttctcca ccgtgctgga ttcgatctgg atgtcgaagc ccttgcgcag caggtacttc
       61 agcatcagca cctggccggt gccgagcaat gagacgccgt agccgacgta gagcttgggc
      121 gacaggtact tgccgatgct gagcacatcg gcacccaatg cacgcgagcc gaccacgccg
      181 gcatcgtcca ggcccagccg ctggccgagt tcggaggcga ccagcgcggt accggcggcc
      241 agcgcggccc gggcggtgcc gatctggcgc gcctcgtcgc cggtcaggct ggacagcgga
      301 cggccgaggg tgaggtaggc cagtgcctcg gattcgctct tcgccgggtt cgaatacacc
      361 gtcgcccgcg gcgcggaggc gcggccttcg acgcggatgc cggcggtcac ctcgccgacc
      421 tggcgctcgg cgcggatgtc cagcaacgga tcgccgaccg gcgtgtccga ccacaacagg
      481 cggccgcggg tgatctgcag ccgctgggcg taggcggtgt agcggccggc aacgtcgagc
      541 gcgccggtgc cgcgcacctc gcgtcccggc agggcgcgca cgcgcagccg cccgcccagg
      601 gtgccgacca ggccgaagcc gttgatcgcg acctgttcgc ccatcaccag ggtgaggtcg
      661 aggtccagcg gattggcgcg ggtgcgctcc ggatccaccg ggtccagcac caccacgtcg
      721 tcggagcggg agacgccctc gtcgaggcgt tccaggtgga tgtcggcctc cggcagggtc
      781 acgctgccgc tgacctgcag cggctcgccc gcgcggtagc gcaccaccac gtccggattc
      841 gcgaccgcac gcaactgccg ggtctccgcg atcagcacgt tggtgccgcg caggttcaat
      901 accagtggcg tgtcctgcgc ctgccagccg aggctgccgt cgacgtccag cacgccgtcg
      961 ccggagcgca cgcggccgac gatgcgcgca ttgccgtccg cctgggcctg caggcgcagg
     1021 tcgccgtcgc gtgcggcgat gccgagcgcc ggcagttcgc tggcgaagcc ctgcaggtgg
     1081 ccctcgccgc cgatcgccgg tgccgcgcgg gtgccgccca gccgcaggtc aacgtccagc
     1141 cggccggtcg gcgcgacgat gtccggcgag aacagctcca tccaggtcag ttcgtcggtg
     1201 ctgaccttga tctcgcccgc cagcggcgcg tagccgtccc agccggtgct gatgcgtgca
     1261 tcgatgcgac catcgtcgtt gaacacggtg ccgaggctga tgccgatcgc ttgcgggtcg
     1321 aaggtggcat cgagctgcag gtcgcggtaa ccggccaggt cgcggcgtgc gcgcgggcgg
     1381 ttgcggatgc cgccggccgc cgaggtgatc gaagcggtgc cgcgccacgc attggccgcc
     1441 ggcacgatgc gcacgcgaag cccggcctcg ccattgaaca cccagggccg gctgtcctcg
     1501 cgctccggca ggtacggcac cagcagcgcc agtggcaatg catcgccctg cagctccacg
     1561 ccacgacgcg gccagtcggc gtccgcgcac agggtgccgc cggtgttcgc cagcaggcag
     1621 gcgcgcgaca gcgcaccgct gctgccatcc cacgaccagc gcgccggttg ctgcagcgtc
     1681 cacgccgcgc ccttggccgg atcgaactgc agcgacgcca gcgcgccctg ccagcgggtg
     1741 ccctgtttgt tcgcatcgcc gcgcagcgcc agcgcgccga attcgctttg cgcgtccgca
     1801 tcgaattgca ggcgttccac cgacccgcgc aagcccgcgc gcaggctgga cagcggcagg
     1861 ccgagttgca ggccctgcgc ctcgaccgcc agcatgccgt tgccggtgcg ccatggcagc
     1921 cggcccttgg cgatgaactg ttcggcgcgg tagtcgccga aggccacgcc ttcgccgcgc
     1981 aggtcgatgt ccacgtccgg tgccgtgcgc gcgccgcgca gggtgaacat gccgcgcatg
     2041 ctgccgcggc catccggcag caggtcgtcc agttgcagcg gcgacaggtt ggcttcgatg
     2101 tcgatgccgg cggcgatctt ggcgcgcgca tcgatgcggc tgccaccgag cgatagcgcg
     2161 acatcgccgg cgtagttgtc gccgtcgatg ttgagcgtgc cgcgcccgcc cagcgcgcgc
     2221 ccgcgcaggg tgccgcccag gttgcccgcc tccgcgtcgg cgagcaggga accgttgtcg
     2281 cggcgcacgc cggaggacag caccacgccg tcgaccgcgc cgggccagtc gggcgcgaag
     2341 tagccggggt cgaagccggc cagggtcgcc ttcgccgtcc acgacagccg cggcgaccat
     2401 gcgaactcgc cgctggcgtc cagccgcccc tgcggcatgc gcgcgcgcag cacgtcgacc
     2461 tggatgcgct cgcgaaagcc gcgcccggcg agatccacgg tcgcgcgctc ctggccgcgc
     2521 tgcagccgcg cctggccctt gaccgcccac cgggcgggcg tgccggcgat gccgaaatcc
     2581 gcatcgccgg cgaccatggt gtcgccgcct tcgccttgcc agcgcaggcc gcgcgcgttg
     2641 acggcgaact tcaacgaagc atcgtccggg tcgcgcaggt cggcgctgcc attggcgttg
     2701 acgcggccac cgaacacgtc cagcaccagt ggctgcagtt gcagcacccg gtcctccagc
     2761 cgcagtttcg atggctgcag ggtggcggcg aagtcgccct gcttcagcgt gccgcgcagg
     2821 ttggcgctgc cgccgatgcc gtccgcgccg aggttgaagg cgagcggcgt cgaggcctcg
     2881 ccgctgccgc cgagcaggcc gatgtcgagc gcgtcgctgt tggcgcgcaa ctgccagcgc
     2941 gggcggtcgg cgtcgccgcg cagggtaagg accgcgcgca ggggcgcggg tgcgcggccg
     3001 ccgatcgcca cgtccagttt cgacaggtcg ccgcgcgcaa tcaggccgaa gcgcggcgcc
     3061 gtgcggccgg ccggcgcggg cagcaccgcg gtggcgcgca ggtcgctgcg gaaattccgg
     3121 cgcgggatgt aatcgccatg cagggtgaag cggccgcggt cgctgtcgac cgccagccgt
     3181 tcgatgtgca gcgaaccggg cgccgcatcc agcccgccac gcgcgctgcg gatgtcgatg
     3241 agcggctcgc cttcctgcac caccttgagg ccatcgatgc ggatcgcacc ggcacgcagc
     3301 gccagcggca gctcgacctg cggcagcacc tccggccagc gcggcaattc gaacggcttg
     3361 tcgctgcgcg gcaggtccag ggtggcgccg gcgatgtcca gcgtgtccag ccgcagggtg
     3421 cggccgagca gcgggcggag cgacggatcc agggtgacct gttcggcgtc gagcacgatc
     3481 cgccccatcg cgcagcgggt ccgggtggtg gccacgcagt cggggtcgcg ctggcgcggc
     3541 accgagaagt gcacgccgcg cagcaccatc ggccccgacg ccggcccctc ggcggtgtcc
     3601 caggtcagct cggtgccggc cggcagccgc gccaccagct cccgcagcag caggtcgcgg
     3661 ccgccgatcg tcatcagcag ccagtacagc agcagggcaa cgccggccgc catcgccagc
     3721 gtggcgatgc cgccgcgcag cgcgagcgtg cgccggcgcg tgcggcgcag ctgccgaagt
     3781 tcggcgatgc gcgcctcgcg ttgttcgccg ctggcatcgg ccggcagtgg cgccagccca
     3841 tggcggtgga cacggtggtc gcgttcgtgc ccgctcacag gtcggcgccg atgttgaggt
     3901 gcagggtgaa cggcgagtcc ggcgacttca gcccgcgcgc gatgtcgacc cgcaccggcc
     3961 ccaccggcga tcgccagcgc acgccgaagc cgaccccggt atgcaggtcc ggcttgctgc
     4021 catcgaatgc gctgccgctg tcgacgaaca ccgccgcccc ccacggaccc ttgaagtaac
     4081 gctcgtactc gatgctggcg gtgaccacgt tgctcgcgcc gaccgcgtag gcgttgccgg
     4141 cggaatcgat gatgcgcggg ccgacctcgc gccagccata gccgcggatg ctgcggtcgc
     4201 caccggcgta gtaacgcagg ctcggcggca gctcgagcag ttcattggtg aaggtgtggc
     4261 cggcctcgcc gcgcaccagc agccggctgt catcctgcat gccatggaac caatgcgcgt
     4321 ccgcgtgcag ctggacgaag ccggtagtgc catccgcgcc accatttcca ccgcggagct
     4381 cgagggtgcc cccggtgccg cggcgcgggg ccaggcggtc gtcgacgtcg atgtattcgg
     4441 cgcgcagcga ggggaaggcg aagctggcgt attcgtacga ctgggtgggc ttggtgatgg
     4501 tgaagtacgc ccagcgctcg cgcagcacgt gcgcggacgc gaccaggttc aggtgctggt
     4561 tgtactgccc gctgcggctg gcgacgaatt cgaggcggcg gttgtcgagc gagtcggtct
     4621 gctcgtccgc ggcctgcagg ctggcggtgt accagccatc cagccaggcg aaggcgggca
     4681 cccggtattg cacggtggcc agcttgcgct tgctcgcgta gtcgatctgg gccagcgcct
     4741 tgtgcccgcg ggcattcagg tagcggcgct cgaagccgag gctgacgccg gcgccgctct
     4801 gggtcccgta gctggcaccg atgctgtaga tgctgcgtgg cgcaggggtc aggtggacct
     4861 gcaccggaac cacgtcgccg gtcttttccg cgggctgcgg cagcacctcg accaggccga
     4921 agtagtccag cgccaccagc gagcgccgca ggcgctccag ctcggcgtcg tcccacggct
     4981 cgccgggctc ccagccgacc agcttgcgca gcagcgaatc gtcgatgacc gcctgcggct
     5041 cctggccaaa gtcgaccgaa cccagcgcgt ggcgcgcgcc gctgcgccag cgcaggtcga
     5101 tgtccgcggc gtgttcggcc cgtgtcaccg cgacccggtg ggcgctgaaa tcggcgtcga
     5161 agtagccacg gccggccagc atgcggctga ccttcgactt gctcgcttcg tacacggcgt
     5221 ggtcgagcac ctcgccgctg cgcggtgcga acccggccag cgctgcggcc acgtgcggat
     5281 cgtcggcgcc cgcaccgtcg atgcccacgt tccaggcgcg cacgcgcacc tggttgccgg
     5341 gcgcgatgct gatcgccacg ctcacgctgc gctcgccgtc gccttcggcg tccgtgcgta
     5401 ccgaggcact ggtgcccgct tcggcatccg cgccctccgg ccgctggcgg tcgctgcgct
     5461 ggatgacgat ggtcggcgtg tagtagccga acggttccag cgcctcgcgg gtttccgcct
     5521 ccgcttcgcg cagcaggtag ctcaagcggc gcgccgacag gtccttgccc agttcgtcgt
     5581 tgagcgacag cgagaggcgc acgttctgcg ccatccgctc gtcggcgagg ccgctgacct
     5641 cgatccgggt caccgtgatc gccgaggccg gcaacgccac gccggcaagg gcaagggcga
     5701 tcagcaggcg ggcggggcgt ggcggcatcg gtgaaggata ccggggcagt gccaaagcgc
     5761 g
//
LOCUS       DAORUK010000132         5749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_16732_length_5749_cov_19.332455, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000132 DAORUK010000000
VERSION     DAORUK010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16732_length_5749_cov_19.332455"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1660
                     /locus_tag="PK818_08540"
     CDS             <1..1660
                     /locus_tag="PK818_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014645937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQY82815.1"
                     /translation="FYQQQGAARFLREIPIATSRGMITDRNGEPLAVSSPVESIWANP
                     QELLKAQARLPQLADALGMDRDELVRRLSQRADKEFVYLKRRINPDEAKAILALEIPG
                     VSSQREYRRFYPQGEAIAHVLGFTNIDDRGQEGLELAFDDWLRGTPGAKRVIRDRRGR
                     IVENVDLVRAAQPGRELALSIDRRIQYLAHRELRTAITETGASSGSAVVLDVATGEVL
                     AMVNLPTFNPNAVGTSPRDAHRNRAVTDLLEPGSTMKPLTVAAGLEAGAITADSLFNT
                     HPGWIANGKYRTTDTHNYGVLDTTGVIRKSSNIGAALIARRLSDAQFDAFLRRFGYGR
                     VTGSGFPGEAAGLFPTPDRWSGTTKQTMSYGYGLNATPLQIATAYAALGNGGLLHQPS
                     FVKGQHATPRQVLDPKIAAEVLRMMQTVTEPGGTATQAAILGYHVAGKTGTSRKASAG
                     GYSRRYIGYFAGLVPVDKPRFSMVVAVNDPTKGSYYGGIVSAPVFRNVMEGALRMMDV
                     APDNLDAWLAAQAEAEARRLKASGHALPADATAAVPAPAARAVQ"
     gene            1657..>3176
                     /locus_tag="PK818_08545"
     CDS             1657..>3176
                     /locus_tag="PK818_08545"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HQY82816.1"
                     /translation="MSRAMLLGELLPELEGLSADLAIAGLVQDSREIRPGDAFVAIAG
                     FGAHGLNFVDAAREAGAAAILFEPPAPAELPPPADAIPVAGLRARMGAMADTFHGHPS
                     AAMTTVGVTGTNGKTSTVQLLAQAWTLRGQKAATIGTLGAGVWPKIVPTGFTTPLVLR
                     MHALLAELRDEGASTVAMEVSSHALDQGRVDGVHFDVAVFTNLTRDHLDYHGDMASYG
                     AAKARLFDWPGLRAATVNLDDGFGRELFEAVGGRVRVFGFSSRGAAGASVRAEEVGLD
                     AGGIRFMLHAGNQAHPVRSPLLGRFNVDNLLGVATTLVALGMAPALVAETLSQLAPVD
                     GRMSRLGGEGGLPLVVVDYAHTPDALEQALASLRAHTAGKLVCVFGCGGERDRGKRPQ
                     MAAIAERGADVAIVTDDNPRGEDGDAIIADIVAGFADRARAIVLRDRARAIAEAIGMA
                     GADDVVLVAGKGHEPYQEINGVRHPFDDTATARELLRRRAQHMDVQVLRPQDAEEQP"
     assembly_gap    3177..3276
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3329..4714
                     /gene="murF"
                     /locus_tag="PK818_08550"
     CDS             3329..4714
                     /gene="murF"
                     /locus_tag="PK818_08550"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HQY82817.1"
                     /translation="MTPRSLAWIAQATSGRLVGEDRLVEAIATDTRALPQGRDALFVA
                     LKGGNFDGHDHVATAALGGCVAALVSREVEASIPQVVVADSERALGDFAAAVQRERAT
                     KVLAITGSNGKTSVKALLLSILGRVGGAYANPGNRNNEIGLPLAVLDAPDDARFAVYE
                     MGAGKPGDIAYLTAIARPDVALVNNIAPAHLQRMGSLLGVADTKAAIYDALADDGVAV
                     VNADDAFAPYFLARAQGRRSVRFGLEASAEISARGIDGDGEGSRFVLVAPAGEVAVAL
                     PMPGRHNVRNALAAAAMALAAGATLGQVRDGLDNVQPVAGRLVSHRLRNGAVLVDDSY
                     NANPGSLDAAIDTLAASGDEAWLVLGDMRELGADEVALHAQAGVRAKAAGLSRLFTLG
                     SLSQAASDAFGDGARGFATHAQLVDALRAELHAGVRLLVKGSRGSAMDRVVKALLAAD
                     AGTAIGEADAA"
     gene            4704..>5749
                     /gene="mraY"
                     /locus_tag="PK818_08555"
     CDS             4704..>5749
                     /gene="mraY"
                     /locus_tag="PK818_08555"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006452808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQY82818.1"
                     /translation="MLLELTRWLEQLQSLFGLFNYLTFRAILGALTALALSLWWGPAM
                     IRYLATLKGGQPIRSDGPQSHFSKAGTPTMGGALILTSIAASVLLWADLRNKYVWVVL
                     VVMLGFGAIGWWDDWIKIVKRDPNGMRSRTKYALQSLLGLAAGIYLFAFADVPAATTF
                     YVPFLKSIALPLAGVGFVAIAYFWIVGFSNAVNLTDGLDGLAIMPTVLVSCALGVFAY
                     ASGNAVFAEYLQIPRVPGAGELVIICAAIAGAGLGFLWFNTYPAMVFMGDIGALSLGA
                     VLGTIAVIVRQELVLVIMGGIFVIETLSVMIQVASFKLTGKRVFRMAPIHHHFELKGW
                     PEPRVIVRFWIISVV"
BASE COUNT          809 a         1965 c         2050 g          825 t
ORIGIN      
        1 gttctaccag cagcagggcg cggcgcgctt cctgcgcgag atcccgatcg ccacctcgcg
       61 cgggatgatc accgaccgca acggcgagcc gctggcggtc agctcgccgg tggaatcgat
      121 ctgggcgaac ccgcaggaac tgctgaaggc gcaggcgcgg ctgccgcagc tggcggacgc
      181 gctgggcatg gaccgcgacg agctcgtccg tcgcctgagc cagcgcgccg acaaggagtt
      241 cgtctacctc aagcgccgca tcaacccgga cgaagcgaag gcgatcctgg cgctggagat
      301 cccgggcgtg tccagccagc gcgagtaccg ccgcttctac ccgcagggcg aggcgatcgc
      361 gcacgtgctc ggcttcacca acatcgacga ccgtggccag gaaggcctgg agctggcgtt
      421 cgacgactgg ttgcgcggca ccccgggcgc caagcgggtg atccgcgacc gtcgtggccg
      481 gatcgtcgag aacgtggacc tggtgcgcgc ggcgcagccg ggccgcgagc tggccctgag
      541 catcgaccgc cgcatccagt acctggcgca ccgcgagctg cgcaccgcga tcaccgagac
      601 cggcgccagc agcggctcgg cggtggtgct cgacgtcgcc accggcgaag tgctggcgat
      661 ggtcaacctg ccgaccttca acccgaacgc ggtcggcacc agcccgcgcg acgcgcaccg
      721 caaccgtgcg gtgacggact tgctggagcc gggttcgacg atgaagccgc tgaccgtggc
      781 cgccggcctg gaagcgggcg cgatcaccgc cgattcgctg ttcaacaccc atccgggctg
      841 gatcgcgaac ggcaagtacc gcaccaccga cacccacaac tacggcgtgc tggacaccac
      901 cggggtgatc cgcaagagct ccaacatcgg cgcggcgctg atcgcccggc gcctgagcga
      961 tgcgcagttc gacgccttcc tgcgccgctt cggctacggc cgggtgaccg gcagtggctt
     1021 ccccggggag gccgccgggc tgttcccgac cccggaccgc tggagcggta ccaccaagca
     1081 gaccatgagc tacggctatg gcctcaatgc caccccgctg cagatcgcca ccgcctatgc
     1141 ggcgctcggc aacggcggcc tgctgcacca gccgagcttc gtcaagggcc agcacgccac
     1201 cccgcgccag gtgctggacc cgaagatcgc ggccgaagtg ctgcggatga tgcagaccgt
     1261 gaccgagccg ggcggcaccg ccacccaggc cgcgatcctc ggctaccacg tggccggcaa
     1321 gaccggcacc tcgcgcaagg ccagcgccgg cggctattcg cgtcgctaca tcggctactt
     1381 cgccggcctg gtgccggtgg acaagccgcg tttctcgatg gtggtcgcgg tcaatgatcc
     1441 gaccaagggc tcctactacg gcggcatcgt ctcggcgccg gtgttccgca acgtgatgga
     1501 aggcgcgctg cggatgatgg acgtggcgcc ggacaacctc gatgcgtggc tggcggcgca
     1561 ggccgaggcc gaagccaggc ggctgaaggc cagcggccac gcgctgccgg cggacgccac
     1621 tgccgcggtg ccggcaccgg cggcgagggc ggtgcaatga gccgcgcgat gctgctcggc
     1681 gaactgctgc cggaactgga agggctgtcc gcggaccttg ccatcgccgg gctggtgcag
     1741 gacagccgcg agatcaggcc gggcgatgcg ttcgtggcca tcgccgggtt cggcgcgcat
     1801 gggctgaact tcgtggatgc cgcgcgcgag gccggcgccg ccgcgatcct gttcgagccg
     1861 ccagcgcccg ccgaactgcc gccgccggcg gatgcgattc cggttgccgg cctgcgcgcg
     1921 cggatgggcg cgatggccga caccttccat ggccacccca gcgcggcgat gaccacggtc
     1981 ggggtgaccg gcaccaacgg caagacctcg accgtgcagt tgctggcgca ggcgtggacc
     2041 ctgcgcgggc agaaggccgc gaccatcggc accctcggcg ccggggtctg gccgaagatc
     2101 gtgccgaccg gtttcaccac cccgctggtg ctgcgcatgc acgcgctgct ggccgaactg
     2161 cgcgacgaag gcgcgtccac ggtggcgatg gaagtgtcct cgcacgcgct cgaccagggc
     2221 cgggtggacg gcgtccattt cgatgtcgcg gtgttcacca acctcacccg cgaccacctc
     2281 gactaccacg gcgacatggc cagctacggc gccgccaagg cgcgcctgtt cgactggccc
     2341 ggcctgcgcg cggccaccgt caacctcgac gatggcttcg gccgtgaact gttcgaggcg
     2401 gtcggtggcc gggtgcgcgt gttcggcttc tcctcgcgcg gcgccgccgg tgccagcgtg
     2461 cgtgcggagg aggtcgggct cgacgccggc gggatccgtt tcatgctgca cgccggcaac
     2521 caggcgcatc cggtgcgctc gccgctgctg ggccgcttca acgtcgacaa cctgctcggg
     2581 gtggcgacca ccctggtcgc gctgggcatg gccccggcac tggtggccga gaccctgtcg
     2641 cagctggcgc cggtcgatgg ccgcatgagc cggctcggcg gcgagggcgg gttgccgctg
     2701 gtggtcgtcg actacgcgca caccccggac gcgctggaac aggcgctggc ctcgctgcgc
     2761 gcgcataccg ccggcaagct ggtctgcgtg ttcggctgcg gcggcgagcg cgaccgcggc
     2821 aagcgcccgc agatggcggc gatcgccgag cgcggcgccg atgtcgcgat cgtcaccgac
     2881 gacaatccgc gcggcgagga tggcgacgcg atcatcgccg acatcgtcgc cggcttcgcc
     2941 gaccgcgccc gcgcgatcgt cctgcgcgac cgcgcgcgcg cgatcgccga ggcgatcggc
     3001 atggccggcg ccgacgacgt ggtgctggtg gccggcaagg gccacgagcc ctaccaggaa
     3061 atcaacggcg tcaggcatcc gttcgacgac accgcgaccg cgcgcgagct gctgcggagg
     3121 cgcgcgcagc acatggatgt gcaagtgctg cgtccgcagg acgcggagga gcagccnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgca gcacatggat gtgcaagtgc
     3301 tgcgtccgca ggacgcggag gagcagccat gacgccgcgt tcgttggcgt ggatcgcgca
     3361 ggcgacgtcg ggccggctgg tcggcgagga ccgactggtc gaagccatcg ccaccgatac
     3421 gcgtgccctg ccgcagggcc gcgatgcctt gttcgtcgcg ctgaagggcg gcaacttcga
     3481 tggccacgac cacgtcgcca cggcggccct gggtggctgc gtggcggcgc tggtgtcgcg
     3541 cgaggtcgag gcatcgatcc cgcaggtcgt cgtggccgat agcgaacgcg cgctcggcga
     3601 tttcgccgcc gcggtgcagc gcgaacgcgc gaccaaggtg ctggcgatca ccggcagcaa
     3661 cggcaagacc agcgtcaagg cgctgctgct ctcgatcctc gggcgggtcg gcggcgccta
     3721 tgccaatccc ggcaaccgca acaacgagat cggcctgccg ctggcggtgc tggacgcgcc
     3781 ggacgacgcc cgcttcgcgg tgtacgagat gggcgcgggc aagcccggtg acatcgccta
     3841 cctcaccgcg atcgcgcggc cggacgtggc gctggtcaac aacatcgcgc cggcgcacct
     3901 gcaacggatg ggcagcctgc ttggcgttgc cgacaccaag gccgcgatct acgacgcgct
     3961 ggcggacgat ggggtggcgg tggtcaacgc cgacgacgcg ttcgcgccgt acttcctggc
     4021 gcgtgcgcag ggccgccgca gcgtccgctt cggactggag gccagcgccg agatcagcgc
     4081 gcgcggcatc gacggcgatg gcgagggctc gcgcttcgtg ctggtcgcgc cggccggcga
     4141 ggttgcggtc gcgctgccga tgccgggccg ccacaacgtg cgcaacgcgc tggccgcggc
     4201 ggcgatggcg ctggccgccg gcgccaccct cgggcaggtg cgtgacggtc tcgacaacgt
     4261 gcagccggtg gcggggcgcc tggtttcgca tcgcctgcgc aatggcgcgg tgctggtcga
     4321 cgacagctac aacgccaacc cgggttcgct ggatgccgcc atcgacaccc ttgccgcaag
     4381 cggcgacgaa gcctggctgg tgctgggcga catgcgcgaa ctcggcgccg acgaagtcgc
     4441 gctgcacgcg caggccggcg tgcgtgcgaa ggcggccggg ctgtcccggc tgttcacgct
     4501 gggcagcctg agccaggccg ccagcgatgc cttcggcgat ggcgcgcgcg ggttcgcgac
     4561 gcatgcgcaa ctggtcgacg ccctgcgcgc cgagttgcac gccggggtgc ggttgctggt
     4621 caaggggtcg cgcggcagtg cgatggaccg ggtggtcaag gcgctgctgg cagcggatgc
     4681 cggcaccgcg attggagagg ccgatgctgc ttgaactgac ccgctggctg gaacaattgc
     4741 agagcctgtt cggcctgttc aattacctga ccttccgcgc catcctcggt gcgctgaccg
     4801 cgctggcgct gtcgctgtgg tgggggccgg cgatgatccg ctacctggcc acgctgaagg
     4861 gcggccagcc gatccgcagc gacggcccgc agtcgcattt ctccaaggcc ggcaccccga
     4921 ccatgggcgg cgccctgatc ctgaccagca tcgccgcctc ggtgctgctg tgggcggacc
     4981 tgcgcaacaa gtacgtgtgg gtggtgctgg tggtgatgct gggcttcggc gcgatcggct
     5041 ggtgggacga ctggatcaag atcgtcaagc gcgaccccaa cggcatgcgc tcgcgcacca
     5101 agtacgccct gcagtcgctg ctcgggctgg cggccggcat ctacctgttc gcgttcgccg
     5161 acgtgcccgc ggcgaccact ttctacgtgc cgttcctgaa gtcgatcgcg ctgccgctgg
     5221 ccggggtcgg gttcgtcgcg atcgcctatt tctggatcgt cggcttctcc aacgcggtca
     5281 acctgaccga cggcctcgat ggcctggcga tcatgcccac cgtgctggtg tcttgcgcgc
     5341 tcggcgtgtt cgcgtacgcc tccggcaacg cggtgttcgc cgagtacctg cagatcccgc
     5401 gggtgcccgg cgccggcgag ctggtgatca tctgcgccgc gatcgccggc gccggcctcg
     5461 gcttcctgtg gttcaacacc tacccggcga tggtgttcat gggcgacatc ggcgcgctgt
     5521 cgctgggcgc ggtgctcggc accatcgcgg tgatcgtgcg ccaggaactg gtgctggtga
     5581 tcatgggcgg catcttcgtc atcgagaccc tgtcggtgat gatccaggtg gcgagcttca
     5641 agctcaccgg caagcgcgtg ttccggatgg cgccgatcca ccaccacttc gaactgaagg
     5701 gctggccgga gccgcgggtg atcgtgcgct tctggatcat cagcgtggt
//
LOCUS       DAORUK010000133         5721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_16847_length_5721_cov_20.456230, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000133 DAORUK010000000
VERSION     DAORUK010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16847_length_5721_cov_20.456230"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1405)
                     /locus_tag="PK818_08560"
     CDS             complement(<1..1405)
                     /locus_tag="PK818_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HQY82819.1"
                     /translation="MSNSGLRAAGAGTPQLTLRAVILAVVLAMILSAANAYLGLFAGL
                     TIATAIPAAVVSMGVLRLLGGGTILENNIVQTGASAGSSIAAGVIFTIPALIILGYWQ
                     DFKYSWVLAIAGLGGLLGVLFSVPLRRSMIVEDPLPFPEGKAAAEVLKAGENPGPGLK
                     ILGISATIGALVKLAAESGLRMIPDNAAGAGFFGKYLGYMGTGLSPALLGVGYIVGLN
                     IGIVVVSGSILAYNIAIPIYHAFFASGDPEMAAKLVGKTAAEAAAIIRGDKIRYLGVG
                     TMLVGGIWTLFSLRNSLLSGVRSGIAAARKGSSLADLPETERDLPMKWMLVALLLFVL
                     PLLGLYQAIVQNWGVSIPMTVIMIVAGFLFVSVSAYLAGLVGSSNNPVSGITIATILF
                     ASVVLMLLLGSDSKIGAVAAIMIGAVVCCAAAVGGDNLQDLKAGYIVGATPWKQQLML
                     GIGAFSCALIMAPVLNLL"
     gene            1594..3378
                     /locus_tag="PK818_08565"
     CDS             1594..3378
                     /locus_tag="PK818_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HQY82820.1"
                     /translation="MPATTTYSDIPLRNDFLGHPKGVYVCFFTEMWERFSFYGMKALL
                     LLYLTKYHLFDDTGGLNLLGAYGGLVYCLPVLGGLLADRWLGMRKAVVFGGVLLVLGH
                     FGMAFEGEQARQVGTAVVRDERALLVFYASLALIIMGVGFLKPNISVIVGRLYADDDP
                     RRDSGFSLFYAGINLGAMMASLVCGVLGETWGWKYGFGAAGVGMIAGLAMFLWGQKYL
                     RGLAEPPRPERLRERVAGIPREWAIYLAALLGLLPIAGLMWATGNNAFTLSAGIDLTL
                     AQLFMLVVLAFVLAWFVRFILRECDRRERAQMLTLMVLIVLAVVFYTLYEQTYGSWVV
                     FTDRMLTKDFFPSLVQAEGLPFAYFVVATLLLPLAFMLAAPWHDRRPGSPLSATLFLL
                     AAAATVLMTVGMVVLQPQTAGSLTFVAGPFILALAPLFSWLWGWLDRRGRNPGKPAKS
                     VLGLLFAALSFLPLVLAAQSAGETGSWASVWWLVLAYGLLQVGEICLYPVGLSAVTQL
                     SVPRVASLMMGTWFLATAFSELLAHKLGTLAAVEDPGALLEHSQAWSAAAATYGDYFR
                     MLLLAGLACTALALLALPWLNRGMHGVR"
     gene            3456..5465
                     /locus_tag="PK818_08570"
     CDS             3456..5465
                     /locus_tag="PK818_08570"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009584246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HQY82821.1"
                     /translation="MPTPRPLALALVLALAAGLAAPDGHAAKKAAPKKAPAVSGVCSD
                     FYANANADWLKANPMPASGAISALGQLTARAQLQQRELLDAAMQSPQGNVQKLLGDFW
                     ASGLDEAAVERDGAGPVAPLLQRIDGIRKTGDVAPAIAALHQVGIPVLFNFTADIDLR
                     DFDRHLGYFSQGGLGLPDPAYYTRGDADTQALLARYADYIRKVLTLTGVPAKDLERET
                     ALVLDLEKRIAAAARPLVELRDPRTNFAAVATAGLGKQYRNLQLDAFLKAQAVSDDAV
                     SIANPAMFAQLDAMVGGLKPAQWKSYLRWRVGDAMAPYLARPWRDASFEFRGKVLLGE
                     GAPAPRWQQVLDAINLAAGPMLGREYAARHLADATRQQAVAIAGQVRDALAASIGNST
                     WLGAAAKAEAQAKLGKLRIEVGTPRRDLDYTVQPMGRGSFGGNMLIASSWRHREEMKR
                     IGRGNADRRWDVLPQQPALAYEPAHNRLIVTAAMLQAPVLDPAMPQAAQYGAFGALVG
                     HELSHAVDARGRMVDAGGNLRDWWDSGEAAAWDANGQRVAALYDTFAYPQLKGVKVNG
                     RLTREENMADIAGLELARAALAAAQPAGGAEAGKAFYRGWAQVWAQQVGMDEAQRRSL
                     QDVRAPGPWRSNGPVMQQAAFGTAFGCKAGTPMQARPADRISILP"
     gene            complement(5480..>5721)
                     /locus_tag="PK818_08575"
     CDS             complement(5480..>5721)
                     /locus_tag="PK818_08575"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010366739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQY82822.1"
                     /translation="LFPNQRVMLLFPPIPMKARTLVIVYGLFELGLGLTGLQPGVAHF
                     AHLGGMLFGWLLIRYWRGQPPFGGPGRKPRMRIVR"
BASE COUNT          890 a         1992 c         1970 g          869 t
ORIGIN      
        1 ccagcaggtt cagcaccggc gccatgatca gcgcgcagga gaacgcgccg atgccgagca
       61 tcagctgctg cttccacggg gtggcgccga cgatgtaacc ggccttgagg tcctgcaggt
      121 tgtcgccacc gaccgccgcc gcgcagcaca ccactgcgcc gatcatgatc gcggcgaccg
      181 cgccgatctt ggagtcgctg cccagcagca gcatcagcac gacggacgcg aacaggatgg
      241 tggcgatggt gatgccggac accgggttgt tcgacgaacc aacgaggccg gcgaggtaag
      301 cggagaccga cacgaacagg aacccggcga cgatcatgat gaccgtcatc gggatgctca
      361 cgccccagtt ctgcacgatg gcctggtaca ggcccagcag cggcagcacg aacaacagca
      421 gcgccaccag catccacttc atcggcaggt cgcgttcggt ctccggcagg tcggccaggc
      481 ttgagccctt gcgggcggcc gcgatgccgc tcctgacgcc ggacagcagc gagttgcgca
      541 gcgagaacag ggtccagatg ccgccgacca gcatggtgcc gacgccgagg tagcggatct
      601 tgtcgccacg gatgatcgcc gccgcttccg ccgcggtctt gccgaccagc ttcgccgcca
      661 tttcaggatc gccgctggcg aagaacgcgt ggtagatcgg gatcgcgatg ttgtaggcga
      721 ggatgctgcc ggacacgacc acgatgccga tgttgaggcc gacgatgtag cccaccccga
      781 gcagcgccgg cgacaggccg gtgcccatgt aaccgaggta cttgccgaag aatccggcgc
      841 ctgcggcgtt gtcagggatc atgcgcaggc cgctttccgc ggccaacttg accagcgcgc
      901 cgatggtcgc ggaaatgccg aggatcttca ggccgggacc cgggttctcg ccggccttca
      961 gcacttccgc ggcggccttg ccctccggga acggcagcgg gtcctcgacg atcatcgagc
     1021 gccgcaaggg caccgagaac agcacgccga gcaggccgcc gaggccggcg atcgccagca
     1081 cccaggaata tttgaagtcc tgccagtagc ccaggatgat cagcgcgggg atggtgaaga
     1141 tcacgcccgc cgcgatcgag gaaccggcgg aggcaccggt ctgcacgatg ttgttctcga
     1201 ggatggtgcc gccgccgagc aggcgcagca cgcccatcga gaccaccgcc gcggggatcg
     1261 cggtggcgat ggtcaggccg gcgaacaggc ccaggtacgc attcgcggcg gacaggatca
     1321 tcgccagcac caccgcaagg atcaccgcgc gcagcgtcaa ctgcggcgta cctgcacccg
     1381 ccgccctcaa gccggaattc gacatcgtca cccctcccag ggcatgcaat cgacgaaacc
     1441 tagcggctgg gggacggcaa gtccagtgcg cgagcccgcg cgacatcgca aggatgcgga
     1501 ccagggccac cccaagtcgc tccccgatac tcgcttggtt ggggtggccc cggcccgcat
     1561 cccccatact tcttcgcatc ccgggggagg cacatgccag caacaacgac ctacagcgac
     1621 atcccgctgc gcaacgactt cctcggccat cccaagggcg tctacgtctg cttcttcacc
     1681 gagatgtggg agcgcttctc gttctacggg atgaaggcgc tgctgctgct gtacctgacc
     1741 aagtaccacc tgttcgacga caccggcggg ctcaacctgc tcggcgcgta cggcggcctt
     1801 gtgtattgcc tgccggtgct cggcggcctg ctcgcggacc gctggctggg catgcgcaag
     1861 gcggtggtgt tcggtggcgt gctgctggtg ctgggccact tcgggatggc gttcgagggc
     1921 gaacaggcgc gccaggtcgg cacggcggtg gtccgcgacg agcgcgcgct gctggtgttc
     1981 tatgcgtcgc tggcgctgat catcatgggc gtgggcttcc tcaagcccaa catctcggtg
     2041 atcgtcggcc ggctgtacgc ggacgacgat ccgcgccggg attccggctt ttcgctgttc
     2101 tacgccggga tcaacctcgg cgcgatgatg gcctcgctgg tctgcggcgt gctcggcgag
     2161 acctggggct ggaagtacgg attcggcgcc gccggcgtcg gcatgattgc cggcctggcg
     2221 atgttcctgt ggggccagaa gtacctgcgc ggcctggccg aaccgccgcg gccggaacgc
     2281 ctgcgcgagc gcgtcgccgg gatcccgcgc gagtgggcga tctacctggc cgcgctgctc
     2341 ggcctgctgc cgatcgccgg gctgatgtgg gcgaccggca acaatgcgtt caccctgtcc
     2401 gcgggcatcg acctgaccct ggcgcaactc ttcatgctgg tggtgctggc cttcgtgctg
     2461 gcctggttcg tgcgcttcat cctgcgcgaa tgcgaccgcc gcgagcgcgc gcagatgctg
     2521 accctgatgg tgctgatcgt actcgcggtg gtgttctaca ccctttacga acagacctac
     2581 ggttcgtggg tggtgttcac cgaccgcatg ctgaccaagg atttcttccc ttcgctggtg
     2641 caagctgaag gccttccgtt tgcctacttc gtagtcgcca cgctgttgct gccgctcgcc
     2701 ttcatgctgg ccgcgccctg gcacgaccgc cgccccggct cgccgttgtc cgccacgctg
     2761 ttcctgctgg ctgcagccgc aaccgtgctg atgacggtgg ggatggtggt gctgcagccg
     2821 cagaccgcgg gctcgctgac gttcgtcgct ggtcctttca tcctggcgct ggcaccgttg
     2881 ttctcgtggt tgtggggctg gctggaccgc cgcgggcgca accccggcaa gccggcgaag
     2941 agcgtgcttg gcctgctgtt cgcggcgctg tcgttcctgc cgctggtgct tgccgcgcaa
     3001 tcggctggcg aaaccggcag ctgggccagc gtgtggtggc tggtgctggc ctacgggttg
     3061 ctgcaggtcg gcgagatctg cctgtacccg gtcgggctgt ccgcggtcac ccagctgtcg
     3121 gtgccgcgcg tcgccagcct gatgatgggc acctggttcc ttgccaccgc attctcggaa
     3181 ttgcttgcgc acaagctggg cacgctggcg gcggtcgaag accccggcgc gttgctggag
     3241 cacagccagg cgtggtcggc tgccgcggcc acctacggcg actacttccg gatgctgctg
     3301 ctggccgggc ttgcatgcac cgcgctcgcc ttgctggcgt tgccgtggct caaccgcggc
     3361 atgcacgggg tgcgctgaac gaggccatct tgatgccccg ggccgcacgt gggccgctgc
     3421 tacactccgg ccactacccg cacgagatcc accgcatgcc gaccccgcgc ccgctcgccc
     3481 tcgccctggt cctcgcgctc gcagccggcc tcgccgcgcc cgacgggcat gcagcgaaga
     3541 aggccgcgcc gaagaaggcg ccagcggtct cgggcgtgtg ttccgacttc tacgcgaatg
     3601 ccaacgcgga ctggctgaag gccaacccga tgcccgccag cggcgcgatc tccgcgctcg
     3661 gccaactgac agcgcgcgcg cagctgcagc agcgcgaact gctggacgcg gcgatgcagt
     3721 cgccgcaggg caacgtgcag aagctgctcg gcgatttctg ggccagcggc ctggacgaag
     3781 ccgcggtcga gcgcgacggc gccggcccgg tggcaccgct gctgcagcgg atcgacggca
     3841 tccgcaagac cggcgacgtc gccccggcga tcgccgccct gcaccaggtc ggtatcccgg
     3901 tgctgttcaa cttcaccgcg gacatcgacc tgcgcgactt cgaccgccac ctcggctatt
     3961 tctcgcaggg cgggctgggc ctgccggatc cggcctacta cacccgcggg gacgccgaca
     4021 cccaggccct gctggcgcgc tacgccgact acatccgcaa ggtgctgacc ctgaccggcg
     4081 tgccggccaa ggacctggaa cgcgagacgg cgttggtgct ggacctcgag aagcgcatcg
     4141 ccgcggccgc gcggccgctg gtggagctgc gcgaccccag gaccaacttc gccgcggtcg
     4201 cgaccgccgg cctcggcaag cagtaccgca acctgcagct ggacgcgttc ctgaaggcac
     4261 aggcggtgag cgacgacgcg gtgtcgatcg ccaacccggc gatgttcgcc cagctcgatg
     4321 cgatggtcgg cggcctcaag ccggcgcaat ggaagagcta cctgcgctgg cgggtcggcg
     4381 atgcgatggc gccgtacctc gccaggccat ggcgcgacgc ctcgttcgag ttccgcggca
     4441 aggtgctgct cggcgagggc gcgcccgcgc cgcgctggca gcaggtgctg gacgcgatca
     4501 acctggccgc cggcccgatg ctcggccgcg agtacgccgc gcgccacctg gccgatgcca
     4561 cccgccagca ggcggtcgcc atcgccggcc aggtgcgcga cgcgctggcc gccagcatcg
     4621 gcaacagcac ctggctgggc gcggcggcga aggcggaagc gcaggccaag ctcggcaagc
     4681 tgcgaatcga agtcggcacc ccgcgccgcg acctcgacta cacggtgcag ccgatgggcc
     4741 gcggcagctt cggcggcaac atgctgatcg cctcgagctg gcggcatcgc gaggaaatga
     4801 agcggatcgg ccgcggcaat gccgatcgcc gctgggacgt gctgccgcag cagccagctt
     4861 tggcctacga gccggcgcac aaccgtctga tcgtgaccgc ggcgatgctg caggcaccgg
     4921 tgctcgaccc cgcgatgccg caagccgcgc agtacggcgc gttcggcgcg ctggtcggcc
     4981 acgaactgag ccacgcggtg gatgcgcgcg gccgcatggt cgatgccggc ggcaacctgc
     5041 gcgactggtg ggacagcggc gaagccgccg cctgggatgc caacgggcaa cgcgttgcgg
     5101 cgctctacga caccttcgcc tatcctcagc tgaagggggt caaggtcaac ggccgcctca
     5161 cccgcgagga aaacatggcg gacatcgccg gcctcgagct ggcccgcgcc gcgctggccg
     5221 cggcgcagcc cgctggcggc gccgaggcgg gcaaggcctt ctaccgcggc tgggcccagg
     5281 tctgggcgca gcaggtgggc atggacgaag cccagcgccg cagcctgcag gacgtgcgcg
     5341 caccgggccc gtggcgcagc aatggcccgg tcatgcagca ggccgcattc ggcactgcct
     5401 tcggctgcaa ggccggcacg ccgatgcagg ccaggccagc ggatcggatc agcatcctgc
     5461 cctgagcagg acgctattcc taacggacga tccgcatcct cggcttgcgc ccgggaccac
     5521 cgaatggcgg ctgcccgcgc cagtagcgga tcagcagcca gccgaacagc atcccgccga
     5581 ggtgggcgaa atgcgccacc cccggctgca ggccggtcag gccaagtccc agctcgaaca
     5641 ggccatacac gatgaccagg gtgcgcgcct tcatcgggat cggcgggaac agcagcatca
     5701 cccgctggtt cgggaacagc a
//
LOCUS       DAORUK010000134         5721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_16848_length_5721_cov_16.814684, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000134 DAORUK010000000
VERSION     DAORUK010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16848_length_5721_cov_16.814684"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /gene="ssrA"
                     /locus_tag="PK818_08580"
     tmRNA           <1..340
                     /gene="ssrA"
                     /locus_tag="PK818_08580"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(624..1298)
                     /locus_tag="PK818_08585"
     CDS             complement(624..1298)
                     /locus_tag="PK818_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQY82823.1"
                     /translation="MTSPLRLAALAATLAAFTAGCATYTGQTSDPDDPNRTRQGALIG
                     AAIGAAAGLLSGGDAVERRQRAMVGAGIGALAGGGIGAYQDRQEAELRRETAGTGIDV
                     TRDGDVIKLNLPDGVTFDFARHDLKPQFYPALNTIASTLKDYDQTIVEVSGHTDSIGS
                     DAANQILSERRANAVSSYLIGQGVVRERFEVVGMGERYPVAGNDTDPGRALNRRVEIR
                     LLPLQG"
     gene            complement(1383..2885)
                     /locus_tag="PK818_08590"
     CDS             complement(1383..2885)
                     /locus_tag="PK818_08590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQY82824.1"
                     /translation="MATSKPRSRRTAEEPARRARREAMSRVDTAWLRMERPTNPMMIT
                     GVLMFSEPMSLARLKRVIEQRFLAYARFRQKPVEGATGAQWVEDETFDLDWHVRLSGL
                     PGKHGPAAEKKALERFASQLASSPLDPTKPLWQFHLVERYQGGSAVVARIHHCYADGI
                     ALVQVLLSLTDTTRDSSRASRLDKAWLKHEAVPVARRVGAIDKYMKLGGKVLEQGMAM
                     YRDPTLAGLIAKEGGELARELMNALALPDDPPSLLRGRLGVSKRVAWADPLDLEEVKA
                     VGRACDCTVNDVLMAAAAGALRSYMLERGEHLEGVTLRATVPVNLRPLEHSRKLGNHF
                     GLVFLELPVGEDNPLRRLERVAECMNNLKNSRQAIVAYGLLAALGVAPPPVQELALEM
                     FSRKASAVATNVPGPQQPLYMAGCTLREMMFWVPQTGSIGIGLSILSYRGQVHFGLIS
                     DARLIPDPDAVIRRFGTEFEKLLYLAMMGNWDQRLDAAGAMALLEDATGA"
     gene            complement(2963..4090)
                     /gene="alr"
                     /locus_tag="PK818_08595"
     CDS             complement(2963..4090)
                     /gene="alr"
                     /locus_tag="PK818_08595"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQY82825.1"
                     /translation="MSEPFDQRPTRIRVDLDALTHNLGRIRAHAGVPVLGVVKANAYG
                     HGLVPVARHLARQGIDQLGVAFVEEGMELRRAGITTPILVMGGIFGPQVAQFIVHDLE
                     ITVSSLDKLRQVEAAAQSLGRQATIHLKIDTGMERIGVHSYSAAPFIEAAVASRWCRL
                     KGIYSHLACADDPASPMTLEQLDRFLDACAHITRIGAPMPVRHLANSGGVLHFPQTHL
                     DMVRPGILLYGVLPDPASIPAIDVRPALSLVSQVVYFKVVRAGHPVSYGATWAPAADT
                     RVVTVPIGYGDGFPRALSSRGQVLVRGQRRQLVGRVCMDQFMVDLGPAGTAYNEDEVV
                     LVGSQQGEAIRAEDLARLAGTIPYEILTGLNQRIPREYRGG"
     gene            complement(4161..5552)
                     /locus_tag="PK818_08600"
     CDS             complement(4161..5552)
                     /locus_tag="PK818_08600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HQY82826.1"
                     /translation="MSARPQQRPFDAKIEQLRVPPQSVDAEQAVLGGLMLDANAFDRI
                     ADQLNDADFYRRDHQLIYRAIRELAEKNKPFDAVTLGEWFDSQGLGEQVAGGAYLVEL
                     ASTTPSAANIKAYAEIVRDKAVLRQLIDVGTGIVNDGFQPEGRDSAELLAKAEQEVFA
                     IAEAGARGKQDFTPVRKALIEAFDVLQTRNANGGGITGLPTGYVEFDEMTAGLQPTDL
                     IILAARPSMGKTTLAINMAEYAALKSKKAVAIFSMEMSASQLALRMISSVGRVNATRL
                     RTGALEDEDWSRVSSAIQLMRDVKVFIDDTPALSPDVLRAKARRLKREHDLGLIVIDY
                     LQLMAVPGNSENRATEISEISRGLKALAKELNVPVIALSQLNRSLETRTDKRPVMADL
                     RESGAIEQDADVIVFIYRDEYYNKENSPDKGLAEIIIGKQRNGPTGALKLKFFGEYTR
                     FDNLAHDMVGSFE"
BASE COUNT          867 a         1933 c         1960 g          961 t
ORIGIN      
        1 ttcgacgggg gtagcgaaat cgcacggcgc atgccgaggg ggtagctttc ctcgttaatc
       61 cagctgcaaa aacccagacg ccaacgacga cgtctacgct ctcgccgctt aaggcgtaag
      121 ccccgaaccc acttgtgcct gtgctcgtgg atgtagggtc atcatcacgg gataggaaac
      181 ggtggcgacc cgccagccgg ctccgaaaca aagcgggtgt ggatggcggc gtgcctcgca
      241 cgttgtgttg ccgccatccg agaccaaaca acgagctaag catgtagtgc cgggcatgga
      301 gcatcttcgg acgggggttc gattcccccc acctccacca acctgaatgg gccgcagcga
      361 tgcggccctt tcttttttcg gccggcctag ctcgacgccc atcgcaggac cggagccgcg
      421 cgcaagcctg ggtgctgcag tggtggccac gatcgaaccg gcgatcgcga ccggccttga
      481 gtcggtcgca agcgggccct gcggcggcag cctgtcggca cagggtccgt cggtgcttgc
      541 aagcggcctc gccgtgtccc catcgggcaa acggaacggg ccgctttcgc ggcccgttcc
      601 gtttgcagca tgccgcgggc ggatcagccc tgcagcggca gcagccggat ctcgacgcgg
      661 cggttgagcg cacggcccgg atcggtgtcg ttgccggcga ccgggtagcg ctcgcccatg
      721 ccgaccacct cgaagcgctc gcgcaccacg ccctgcccga tcaggtagct ggagaccgcg
      781 ttggcgcggc gctccgacag gatctggttg gcggcatcgg agccgatgct gtcggtgtgg
      841 ccgctgacct cgacgatggt ctggtcgtag tccttcaacg tgctggcgat ggtgttgagc
      901 gccgggtaga actgcggctt gaggtcatgc ctggcgaagt cgaaggtcac gccgtccggc
      961 aggttgagct tgatgacgtc gccgtcgcgc gtcacgtcga tgccggtgcc agcggtttcg
     1021 cggcgcagtt cggcttcctg gcgatcctgg taggcgccga tgccgccacc ggcgagcgca
     1081 ccgatgccgg cgccgaccat cgcgcgctgg cggcgctcca cggcatcgcc gccactgagc
     1141 aggcccgcgg ccgcgccgat cgcagcgccg atcagggcac cctgcctggt gcggttgggg
     1201 tcatccgggt cgctggtctg gccggtatag gtcgcgcagc cggcggtgaa cgccgccagc
     1261 gttgccgcaa gtgcggcgag tctgaggggg gacgtcatgg catggctcct tgggggcgtg
     1321 gcagaagacg cccgaacgtt aggtgcccgc gtctggccct gacgtgaatg gggcaaccgc
     1381 cgttaagccc cggttgcgtc ttccagcagc gccatggccc cggcggcatc caggcgctgg
     1441 tcccagttgc ccatcatcgc caggtacagg agcttctcga actcggtgcc gaagcggcgg
     1501 atcaccgcgt ccggatccgg gatcaggcgc gcatcggaaa tcaggccgaa gtggacctgg
     1561 ccgcggtagc tgaggatcga caggccgatg ccgatggacc cggtctgcgg gacccagaac
     1621 atcatctcgc gcaaggtgca gccggccatg tacaggggct gctgcggtcc cgggacattg
     1681 gtcgcgaccg cgctcgcctt gcggctgaac atctccagcg cgagctcctg taccggtggc
     1741 ggcgccaccc caagtgcagc caaaagacca tatgcaacaa tagcttggcg tgagtttttc
     1801 aggttattca tgcactcggc gacgcgctcc aggcgccgca atggattgtc ctcgccaacc
     1861 ggcaattcga ggaacaccag gccgaagtgg ttgcccagct tccgcgaatg ttcgagcggg
     1921 cgcaggttga ccggcaccgt cgcgcgcagg gtcacgcctt cgaggtgctc gccgcgctcc
     1981 agcatgtagc tgcgcagggc gccggcggcc gcggccatca gcacatcgtt gaccgtgcaa
     2041 tcgcaggcac gcccgaccgc cttcacctcc tccaggtcca gcgggtcggc ccatgccacc
     2101 cgcttgctga ccccgagccg gccacgcagc agcgagggag ggtcgtccgg cagggccagc
     2161 gcgttcatca gctcgcgcgc gagttcgccg ccttccttcg cgatcagccc ggccagggtc
     2221 ggatcgcggt acatcgccat cccctgctcg agcaccttgc cgcccagctt catgtacttg
     2281 tcgatcgccc ccacccggcg cgccaccgga accgcctcgt gcttcagcca ggccttgtcg
     2341 aggcggctgg cgcgcgacga atcgcgggtg gtatcggtca gcgacagcag cacctgcacc
     2401 agcgcgatgc cgtccgcata gcaatggtgg atgcgcgcga ccaccgccga accgccctgg
     2461 taacgctcca ccaggtggaa ttgccacagt ggcttggtcg ggtccagcgg gctggaggcg
     2521 agctggctgg cgaagcgctc cagcgccttc ttctccgcgg cggggccatg cttgcccggc
     2581 agccccgaca ggcgcacgtg ccagtcgagg tcgaaggtct cgtcctccac ccactgcgcg
     2641 ccggtggcgc cttccaccgg cttctgccgg aaccgcgcat atgccaggaa gcgctgttcg
     2701 atcacccgct tgagccgtgc cagcgacatc ggttcgctga acatcagcac gccggtgatc
     2761 atcatcgggt tggtcggccg ctccatccgc agccacgcgg tgtccacccg cgacatcgcc
     2821 tcgcgcctgg cgcgccgcgc gggctcttct gcagtgcgcc tggagcgcgg tttcgacgtg
     2881 gccatgccaa cctccccggg ggtgacgcga gtgaatcacg cccttgcgcg ggcggtcaac
     2941 gcgcaggagc ggcgcagtgc gctcagccgc cgcgatattc gcgcgggatg cgctggttga
     3001 ggccggtgag gatctcgtac gggatggtgc cggcgagccg ggccaggtct tctgcgcgaa
     3061 tcgcctcgcc ctgctgggag ccgaccagga ccacttcgtc ctcgttgtag gcggtgcctg
     3121 cggggccgag gtcgaccatg aactggtcca tgcacacgcg cccgaccaac tgccgtcgct
     3181 gcccgcgcac cagcacctgc ccgcgcgagg acagtgcccg cgggaatccg tcgccgtagc
     3241 cgatcgggac cgtcaccacc cgggtatcgg cggccggcgc ccaggttgcg ccgtagctca
     3301 ccgggtgccc ggcccgcacc accttgaagt acacgacctg cgaaaccagc gaaagcgccg
     3361 gccgcacgtc gatggcggga atcgaggcgg gatcgggcag caccccgtac agcaggatcc
     3421 cgggccgcac catgtccagg tgggtctgcg ggaaatgcag cacgccgccg gagtttgcga
     3481 ggtggcggac cggcatcggc gcgccgatcc gcgtgatgtg tgcacatgcg tccaggaagc
     3541 ggtccagctg ctccagcgtc atcggcgacg ccgggtcgtc ggcgcaggcc aggtgcgaat
     3601 agatgccctt cagccggcac cagcgggatg cgaccgccgc ctcgatgaag ggcgctgccg
     3661 aatagctgtg cacgccgatc cgttccatcc cggtgtcgat cttgagatgg atggtcgcct
     3721 gccggcccag cgactgcgcg gcggcctcga cctggcgcag cttgtccagc gaggacaccg
     3781 tgatctcgag gtcgtggacg atgaactgcg cgacctgcgg gccgaagatc ccgcccatca
     3841 ccaggatcgg ggtggtgatc ccggccctgc gcagttccat gccttcctcg acgaaggcca
     3901 cgccgagctg gtcgatgccc tgccgcgcaa ggtgccgtgc caccggcacc aggccatggc
     3961 cgtaggcatt ggccttgacc acgcccagca ccggcacgcc ggcatgcgcg cggatgcggc
     4021 ccaggttgtg ggtcagcgca tcgaggtcga cccggatgcg ggtggggcgc tggtcgaagg
     4081 gttcgctcat ggcgatattc gaacatatcc gggcgccacc cacggcatgc ccgcgccgtg
     4141 gagggaaccc gcgcggccgg ctactcgaag ctgcccacca tgtcgtgggc gaggttgtcg
     4201 aagcgggtgt attcgccgaa gaacttcagc ttgagcgcgc cggtggggcc gtttcgctgc
     4261 ttgccgatga tgatttcggc gagccccttg tccggcgaat tttccttgtt gtagtactcg
     4321 tcgcggtaga tgaacacgat cacgtccgcg tcctgctcga tcgcgccgga ctcgcgcagg
     4381 tcggccatca ccgggcgctt gtcggtgcgc gtttccagcg agcggttgag ctgggaaagc
     4441 gcgatcaccg gcacgttgag ttccttcgcc agcgccttca ggccgcgcga gatctcggag
     4501 atctcggtcg cgcggttctc gctgttgccg ggtaccgcca tcagctgcag gtagtcgatc
     4561 acgatcaggc cgaggtcgtg ctcgcgcttc aggcgccgcg ccttggcccg cagcacgtcg
     4621 ggcgacagcg ccggggtatc gtcgatgaac accttgacgt cgcgcatcag ctggatcgcg
     4681 ctggacacgc ggctccagtc ctcgtcctcg agcgcgccgg tgcgcaggcg ggtggcgttg
     4741 acccggccga ccgaggagat catgcgcagc gccagctggc tggcggacat ttccatcgag
     4801 aagatcgcga ccgccttctt cgacttcagc gcggcgtact cggccatgtt gatggccagc
     4861 gtggtcttgc ccatcgacgg acgcgcggcg aggatgatca ggtcggtggg ctgcaggccg
     4921 gcggtcattt catcgaattc gacgtacccg gtgggcagcc cggtgatccc gccgccgttg
     4981 gcgttgcggg tctgcagcac gtcgaacgcc tcgatcagcg ccttgcgcac cggggtgaag
     5041 tcctgcttgc cgcgcgcacc ggcctcggcg atggcgaaca cctcctgctc cgccttggcc
     5101 agcagttcgg cggagtcccg gccctccggc tggaagccgt cgttgacgat gccggtgccg
     5161 acgtcgatca gctgccgcag caccgccttg tcgcgcacga tctcggcata ggccttgatg
     5221 ttcgccgcgc ttggggtggt gctggccagc tccaccaggt aggcgccgcc ggcgacctgt
     5281 tcgccgagcc cctgcgagtc gaaccactcg cccagggtca ccgcgtcgaa cggcttgttc
     5341 ttctccgcca gttcgcggat cgcgcggtag atcagctggt ggtcgcggcg atagaaatcg
     5401 gcatcgttga gctggtcggc gatgcggtcg aacgcgttcg cgtcgagcat caggccgccg
     5461 agcacggcct gctcggcatc cacggattgc gggggcaccc gcagttgctc gatcttggcg
     5521 tcgaacgggc gttgctgcgg acgtgcgctc atcaggtgga aggctccccg ttgcgggccg
     5581 tcatcgcgga ccgcttgtta ttgttcgatg gcaggcatcg tagggcgcat cgagcgcgga
     5641 gggttgcaga caagtcgtgg gacatcctgt ggataacccg tctcaccggc tgcgacgggc
     5701 gagaaccgag tagcgccacg c
//
LOCUS       DAORUK010000135         5693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_16979_length_5693_cov_20.509578, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000135 DAORUK010000000
VERSION     DAORUK010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16979_length_5693_cov_20.509578"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PK818_08605"
     CDS             complement(<1..893)
                     /locus_tag="PK818_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQY82827.1"
                     /translation="MSEVATSITYKRLRQYAKPYWGLIAIGMLAMALEAAASAGTLKL
                     LGPIVDDIFVEKAFSYWLPAGLVILLLARGLFGLAADYTIARSGRGVARDLRMQLMDK
                     YLHLPGARFDTEPVPSMLTRLGADSEQVAQAAVDALKVMASNTLGILATLAVMFWTSW
                     RVSLVLLLLAPAMAWMMGKVGRRYRRLNHAIQESAAGMLQTADQALHAQQDVKAYGAQ
                     PAEMARYGEQTLQNLRLALKIEVTRGTLSMLVQGLGAVGVAVMLVAAGFEAAQGRLTA
                     GDFMTLMTAMVSLVPMLRQLT"
     gene            complement(890..1324)
                     /locus_tag="PK818_08610"
     CDS             complement(890..1324)
                     /locus_tag="PK818_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQY82828.1"
                     /translation="MRIRDFRDDDTPEINLIPLIDVLLCLLIFFVVTTTFDARSVLKL
                     ELPRADGSPSQAQSKALSVLVNAQGRYFVGDREVLRTDLESLKQSLRDVAGDDRERAV
                     LLRADARTPHQSVVTALDALGQLGFRRVAIATAPSPGGASAR"
     gene            complement(1332..1982)
                     /locus_tag="PK818_08615"
     CDS             complement(1332..1982)
                     /locus_tag="PK818_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005916730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQY82829.1"
                     /translation="MLELIRAGGWPMIPLLLLSALALAIIVERFWSLRRERVLPPGLG
                     DEVRAWVARGKSLDPAHVDSLRSTAPLGALLAAALDVRNRSREEIRERVEDVGRHLVH
                     RMERYLNTLGTIAAAGPLLGLFGTVVGMIEMFLGILDHGIGDANQLAGGIGKALVCTA
                     TGMLVAIPALAFHRYFRGRIAGYIVDMEHEAIRLMDVLDAAPAPAAPGTNPAVKPG"
     gene            complement(2001..4382)
                     /locus_tag="PK818_08620"
     CDS             complement(2001..4382)
                     /locus_tag="PK818_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HQY82830.1"
                     /translation="MSNDSPFSGPTCAAALLAGVCACLWWPFLPAWPLLVMSLLAGIA
                     LAWRARRARVLGVLLLGFGFAGLHAAAALHRQIPAALEGKAHEVSGRIVGLPLHEVRR
                     TRFEFVVDADPTQPPALRGKRLRIGSFDDDPRNRAGLVAGSRWRFHVQLRVPQALRNP
                     GGNDGEMQAMAARIAATGYVLEPALALRLAAPTGIDAWRERMSGRIAAALPVASARFI
                     RALALGDTRGLDDRDWSRLRAAGLTHLIAISGFHVGLVAGFFALFAAAAWWVVPGLCR
                     HVPRPFASGFAAVAGAFAYAAMTGFALPTLRTAVMIAALVAARCLRRRQRLADTLALG
                     CIVLLLLDPLAVLGAGFWLSFAGVAWLLWCLPPDQGRRRWAMLRGFVAAQAVATLGLL
                     PLTVMLFGQASFAGPLANLAAVPWWSLVVIPLALLGVLAESLHGGWGEAGWWLSAQAF
                     DLLWPALARIADSPLAMAWLPEPRWFALPLALLSAFWLLLPRGVPGKPLALLLGLPLL
                     HPPLDLPRHGEVELVVIDVGQGLSVLVRTARHSLLYDMGPAMRDGFDAGERAVVPALH
                     ALGVRRLDAAVLSHGDNDHAGGWASVAKAFPVAALLAPAGSPAPGITACSAGQSWEWD
                     GVRFRFLHPTPWFPYLGNEASCVLRIESAHGSALLTSDIGNVVESKLLRVAPRALRTD
                     VLVVPHHGSAGSSSKAFVAATRPRLAVVSSGAGNRFGHPRAEVVRRWCDGGAEVLDTA
                     RAGAVRIWLRQAGVQRPGLLVDERRMARPRLWDAARRRHGTAGLCYAPEMQRP"
     gene            complement(4595..5314)
                     /gene="lolD"
                     /locus_tag="PK818_08625"
     CDS             complement(4595..5314)
                     /gene="lolD"
                     /locus_tag="PK818_08625"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HQY82831.1"
                     /translation="MSEQALDACGDEVIRAEGLAKTYAEGSLHTRVFDGLDIAVRAGE
                     TVAILGASGAGKSTLLHLLGGLDVPSAGEVYVTGRKMSALSDAERGRLRNRALGFVYQ
                     FHHLLPEFTALENVMMPVLLAGQATSEAVRRAQALLESVGLGHRLQHKPGELSGGERQ
                     RAAVARALVNAPACVLGDEPTGNLDEKTAAHVFAQMLELNRAQRTSLVLVTHDRSLAR
                     RLDRVLELHEGRLRELATHEV"
     gene            complement(5307..>5693)
                     /locus_tag="PK818_08630"
     CDS             complement(5307..>5693)
                     /locus_tag="PK818_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQY82832.1"
                     /translation="LVSSQVMLVTDKQADIAILRTLGLTPRQVMQVFMVQGSLIGVIG
                     TVAGVLGGILLTLNLDRILQAIERVLGVQLLPQDVYYITGLPTDLQAGDVAAIACVAL
                     AMAFVATLYPAWRAARTAPAEALRYE"
BASE COUNT          914 a         2144 c         1932 g          703 t
ORIGIN      
        1 ttggtgagct ggcgcagcat cggcaccagg ctgaccatcg cggtcatcag ggtcatgaag
       61 tcgccggcgg tcaaccggcc ctgcgcggcc tcgaagccgg ccgcgaccag catcaccgcc
      121 accccgaccg cgcccagtcc ctgcaccagc atcgacaggg tgccgcgggt gacctcgatc
      181 ttcagcgcca gccgcaggtt ctgcagggtc tgctcgccgt agcgcgccat ttccgccggt
      241 tgcgcgccgt aggccttgac gtcctgctgc gcatgcaggg cctggtcggc ggtctgcagc
      301 atgcccgccg cggattcctg gatggcgtgg ttcagccgcc ggtagcgccg gcccaccttg
      361 cccatcatcc aggccatcgc cggcgccagc agcagcaaca ccagcgaaac ccgccagctg
      421 gtccagaaca tcaccgcgag cgtggcgagg atgcccaggg tgttgctggc catcaccttc
      481 agtgcatcca ccgcggcctg cgccacctgc tcgctgtcgg cgcccaggcg ggtcagcatc
      541 gacggcaccg gctcggtgtc gaagcgcgcc cccggcaggt gcaggtactt gtccatcagc
      601 tgcatgcgca ggtcgcgcgc caccccgcgc ccggagcggg cgatcgtgta gtccgcggcc
      661 aggccgaaca gcccgcgcgc cagcagcagg atcaccaggc cggccggcag ccagtagctg
      721 aacgccttct cgacgaagat gtcgtcgacg atcggcccga gcagcttcag ggtcccggca
      781 ctggccgcgg cttccagcgc catcgccagc atgccgatcg cgatcagtcc ccaatacggc
      841 ttcgcgtatt ggcgcaggcg cttgtaggtg atggaggtcg cgacctcgct caccgggcgc
      901 tggccccgcc cgggcttggc gcggtggcga tcgcgaccct gcggaagccg agctggccca
      961 gcgcgtcgag ggcggtcacc accgactggt gcggggtgcg tgcatccgcg cgcagcagca
     1021 ccgcgcgctc gcggtcgtcg ccggcgacgt cgcgcagcga ctgcttcagg gactccaggt
     1081 cggtgcgcag cacctcgcgg tcgccgacga agtagcgccc ctgcgcattg accagcacgc
     1141 tcagcgcctt cgattgcgct tgcgaagggc tgccatcggc gcgcggcaat tccagcttca
     1201 ggaccgagcg cgcatcgaag gtggtggtga ccacgaagaa gatcagcagg cacagcagca
     1261 cgtcgatcaa cgggatcagg ttgatctccg gggtatcgtc gtcgcgaaag tccctgatcc
     1321 gcacgcgcgc ctcagcctgg cttcacggcc gggttcgtcc ccggcgcagc cggcgcgggg
     1381 gcggcgtcga gcacgtccat gaggcggatc gcctcgtgct ccatgtcgac gatgtagccg
     1441 gcgatgcggc cgcggaaata gcggtggaag gccagcgcag ggatcgccac cagcatcccg
     1501 gtggcggtgc acaccagcgc cttgccgatg ccgccggcca gctggttggc gtcgccgatg
     1561 ccgtggtcga ggatgccgag gaacatctcg atcatgccga ccacggtccc gaacaggccc
     1621 agcaacggcc ccgccgcggc gatcgtgccc agcgtgttca ggtagcgctc catccggtgc
     1681 accaggtggc ggcccacgtc ctccacccgc tcgcggatct cttcgcggct gcggttgcgc
     1741 acgtccagcg cggccgccag cagcgcgccc agcggcgcgg tggagcgcag cgaatcgacg
     1801 tgcgccggat ccagcgactt gccgcgcgcg acccacgccc gcacctcgtc gccaagcccc
     1861 ggcggcagca cgcgttcccg gcgcaggctc cagaagcgct cgacgatgat cgccagcgcc
     1921 agcgccgaca gcagcagcag cgggatcatt ggccagccac cggcccggat cagttccagc
     1981 acgcgccaat cctccggccc tcacggccgt tgcatctccg gcgcgtagca tagcccagcc
     2041 gttccgtgcc gccgtcgcgc cgcatcccac aagcgcggcc gggccatgcg gcgttcgtcc
     2101 accagcaggc ccgggcgctg cacccctgcc tgccgcagcc agatccgcac cgcgcctgcg
     2161 cgcgccgtat ccagcacttc cgcgccacca tcgcaccagc gccgcaccac ctccgcgcgc
     2221 ggatggccga agcggttgcc ggcgccggac gacaccaccg ccagccgcgg ccgggtggcg
     2281 gcgacgaagg ccttgctgga ggagccggcg ctgccgtggt gcgggaccac cagcacgtcg
     2341 gtgcgcaggg cccggggggc gacacgcagg agtttcgatt cgacgacgtt gccgatgtcg
     2401 ctggtcagca acgcgctgcc gtgcgcactt tcgatgcgca acacgcaact ggcttcgttg
     2461 ccgagatagg ggaaccaggg cgtcggatgc aggaagcgga aacgcacgcc gtcccattcc
     2521 caggactgcc cggcgctgca cgccgtgatc ccgggcgcgg ggctgccggc cggcgccagc
     2581 agcgccgcca ccgggaacgc cttcgccacc gatgcccagc cgccggcgtg gtcgttgtcg
     2641 ccatggctga gtacggccgc atccagcctg cgcacgccca gcgcgtgcag ggccggcacc
     2701 accgcacgtt cgccggcatc gaagccgtcc cgcatcgccg gccccatgtc gtacagcagg
     2761 ctgtggcgcg cggtccgcac caggaccgac agcccctgcc cgacatcgat caccaccagc
     2821 tccacttcgc catggcgcgg caagtccagc ggcggatgca gcaatggcaa ccccagcaac
     2881 agcgccagcg gcttgccggg cactccgcgc ggcagcagca gccagaacgc cgacagcagt
     2941 gccagcggca acgcgaacca gcgcggctcc ggcagccagg ccatcgccag cgggctgtcg
     3001 gcgatccgcg cgagcgccgg ccacagcagg tcgaaggcct gcgcggacag ccaccagccg
     3061 gcttcacccc acccgccatg caggctttcc gcaagcacgc ccagcaaggc cagcggaatc
     3121 accaccaggc tccaccacgg caccgccgcc aggttggcca gcgggccggc gaacgaggcc
     3181 tgcccgaaca gcatcacggt cagcggcagc aagcccagcg tggccaccgc ctgcgccgcg
     3241 acgaatccgc gcagcatcgc ccagcgtcgc cgcccctggt ccggcggcag gcaccacaac
     3301 agccacgcca ccccggcaaa gctgagccag aatcccgcgc ccagtaccgc cagcggatcc
     3361 agcagcagca ggacgatgca gcccaacgcg agggtgtcgg caagccgctg gcggcgtcgc
     3421 aggcagcgcg ccgcgaccag cgccgcgatc atcaccgcgg tacgcagggt cggcagcgcg
     3481 aagccggtca tcgccgcata cgcaaacgca ccggccaccg ccgcgaagcc ggaggcgaat
     3541 ggccgcggca cgtggcggca caggccggga acgacccacc acgccgccgc cgcgaacagc
     3601 gcgaagaagc ccgcgaccag gccgacgtgg aagccggaga tcgcgatcag gtgggtgagc
     3661 ccggccgcgc gcaggcgcga ccagtcgcga tcgtcgaggc cacgggtatc gccaagcgcc
     3721 agcgcacgga tgaagcgcgc ggaagccacc ggcagcgccg ccgcgatccg cccgctcatg
     3781 cgttcgcgcc aggcatcgat cccggtcggt gccgcgagcc gcagcgccag cgccggttcc
     3841 agcacgtagc cggtggccgc gatgcgcgcc gccatcgcct gcatttcgcc atcgttgcca
     3901 ccggggttgc gcagcgcttg cggtacccgc agctgcacgt ggaagcgcca gcggctgccg
     3961 gcgaccaggc cggcccggtt gcgcggatcg tcgtcgaacg acccgatccg caaacgcttg
     4021 ccgcgcagcg ccggcggctg ggttggatcg gcatccacca cgaattcgaa gcgggtgcgg
     4081 cggacttcgt gcagcggcag gccgacgatg cgcccgctca cctcatgcgc cttgccctcc
     4141 aacgcggccg ggatctgccg gtgcagcgcg gccgcggcgt gcagcccggc gaagccgaag
     4201 ccgagcagca agacccccag cacccgcgcg cgccgcgcgc gccacgccag cgcgatcccg
     4261 gcgagcaacg acatgaccaa cagcggccat gccggcagga acggccacca caggcaggcg
     4321 cacacgccag ccagcagggc cgcggcgcag gtgggaccgg agaatggaga gtcattgctc
     4381 acgccgacag cgtggcgatg ctcccgatga ttcgcgatcg gcaatgaacc cgctgtttcc
     4441 tcagggaact ccgacatccg cggaatcgtc cgggccgggc ccgggtgggc aaggttcgag
     4501 cgcgccatgg atcacgcgcg gccgcgcatc ggagccggat gcgaagcctg agcagcgaac
     4561 attgcccgcc ggggaccggc ccggacgcga cgggctagac ctcgtgcgta gccagttccc
     4621 gcagcctgcc ctcgtgcaac tccagtacgc gatccagcct gcgcgccagg ctgcggtcgt
     4681 gggtcaccag caccaggctg gtgcgctggg cgcggttgag ctccagcatc tgcgcgaaca
     4741 cgtgcgccgc ggtcttctcg tcgaggttgc cggtgggttc gtcgccaagc acgcaggccg
     4801 gcgcgttgac cagcgcacgc gccaccgccg cgcgctggcg ctcgccgccg gacagttcgc
     4861 ccggcttgtg ctgcaggcga tggcccaggc ccaccgattc cagcaatgcc tgcgcgcgcc
     4921 gcaccgcctc ggacgtggcc tgcccggcca gcagcaccgg catcatcacg ttctcgagcg
     4981 cggtgaactc gggcaacagg tggtggaact ggtagacgaa gccgagcgcg cggttgcgca
     5041 ggcggccgcg ttcggcgtcg gacagcgcgc tcatcttgcg accggtcacg tacacctcgc
     5101 cggcactggg cacgtcgagg ccgccgagca ggtgcagcag cgtgctcttg ccggcgccgg
     5161 aggcaccaag gatggccacg gtctcgcccg cgcgcaccgc gatgtccagc ccgtcgaaca
     5221 cccgcgtatg caggctgcct tcggcatagg tcttggcgag gccctcggcg cggatcacct
     5281 cgtcgccgca cgcgtccaat gcttgctcac tcatagcgca gcgcctccgc gggggcggtc
     5341 cgcgccgccc gccaggccgg gtagagggtg gccacgaacg ccatcgccag cgccacgcag
     5401 gcgatcgcgg cgacgtcgcc cgcctgcagg tcggtcggca ggccggtgat gtagtagacg
     5461 tcctgcggca gcaattgcac gccgagcacg cgttcgatcg cctgcaggat gcggtccagg
     5521 ttgagggtca gcaggatgcc gccaagcacg ccggccacgg tcccgatcac cccgatcagg
     5581 ctgccctgca ccatgaacac ctgcatcacc tgccgcgggg tcaggccaag ggtgcgcagg
     5641 atcgcgatgt cggcctgctt gtcggtcacc agcatcacct gcgacgacac cag
//
LOCUS       DAORUK010000136         5679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_17044_length_5679_cov_16.943101, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000136 DAORUK010000000
VERSION     DAORUK010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17044_length_5679_cov_16.943101"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PK818_08635"
     CDS             complement(<1..1033)
                     /locus_tag="PK818_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HQY82833.1"
                     /translation="MRVRSCKRPHVSDSASNPALAADDNTPEKRDFIRSIVREDLASG
                     RHAAIRTRFPPEPNGYLHIGHAKAICLDFGIAAEFGGDCNLRLDDTNPAKEDPEYVRA
                     IQDDVRWLGFDWHSLRHASDYFEVFYLAAEKLIRQGDAFVCDLSADDVRAYRGSLTEP
                     GRNSPYRERSPEENLDLFRRMRAGEFVDGARTLRAKIDMASGNINLRDPALYRIKHVE
                     HQNTGSEWPIYPMYDYAHSLSDAVEGITHSLCTLEFEDHRPLYDWCVDKVDLAHSPEL
                     VQPLTDRGLPFEAAKPRQIEFSRLNFNWLVMSKRKLLQLVESGRVHGWDDPRMPTLQG
                     IRRRGYTPSA"
     gene            1100..1363
                     /locus_tag="PK818_08640"
     CDS             1100..1363
                     /locus_tag="PK818_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HQY82834.1"
                     /translation="MRVVYEAANVIDAHLVRQALEHAGLPAWVRGEALTGGMGELGVF
                     GLVAVMVPDDCVPAAREVVEALQLGEPLQALDDEDDLPGTVPA"
     gene            1360..2280
                     /locus_tag="PK818_08645"
     CDS             1360..2280
                     /locus_tag="PK818_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQY82835.1"
                     /translation="MTAAARAREAAIAQLLVGAAIIGTNGLMVRLAGMPPTAVAFWRM
                     LLAGLMLGALVLARHGWQPLSRRAWWWCAVPALAFAIDLWLWHRSILLVGPGLATLLA
                     NAQVFFMALAGVLLFREPLGLRFACGVLLAFFGLWLMLGGDWASLPADYRWGVWLGLA
                     TGLAYAVYNIGIKRSQGVAAAAKARAPVEQVLCIAAFGSALCLGLMGWAEGSAFTPPD
                     LRAWGVLLVLAAVGHCLSWVLISRAMVALPVALAGLLLLAQPIVAYLLDVALFDLPTS
                     PRQWLGLVVSLAGIFVAGMKSRSVTVADEP"
     gene            complement(2293..2907)
                     /locus_tag="PK818_08650"
     CDS             complement(2293..2907)
                     /locus_tag="PK818_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82836.1"
                     /translation="MTLVCVLVWWQATRPATTPATTAAGSAATRGCPLPPRIQPGDPP
                     LQTPVPGGMALPAIGDARLQPLAGFSIQARVLGREDYRLGREAEFSPTDLALGWGRMT
                     EDAVLSHLQISQSSRWYRYRWSDAPPIPLQEIIRSSANMHMIPADDAVAAALRRIERG
                     DRVRIDGWLVQVEGEDWRWRSSLTRDDSGGGACELVYACSITPE"
     gene            complement(2954..3859)
                     /gene="oxyR"
                     /locus_tag="PK818_08655"
     CDS             complement(2954..3859)
                     /gene="oxyR"
                     /locus_tag="PK818_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HQY82837.1"
                     /translation="MNLRDLKYLVALADLRHFGKAADACFVSQPTLSTQIRKLEDELG
                     VALVERAPRKVMLTAAGQDVVLRARRIVAEVEQMKESARRSRDPESGSMRLGVFPTLG
                     PYLLPHIVPQLRERFPQLELLLVEEKSDVLLQRLRDGKLDAALLALPVHDEQLHAELL
                     FEEPFVFAAPQRHPLAARASVSMAQLSDETLLLLEDGHCLRDQALDVCRLSGANEKSG
                     FRATSLETLRQMVAAGVGVTLLPALSVHAPVAQPPNIRVVPFRKPAPSRRIALVWRKS
                     SALDGFLHTLADAIGPLARAQLPAL"
     gene            complement(3881..5467)
                     /gene="ahpF"
                     /locus_tag="PK818_08660"
     CDS             complement(3881..5467)
                     /gene="ahpF"
                     /locus_tag="PK818_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HQY82838.1"
                     /translation="MLDQDLKTQLAAYLEKLQQPIELVASLDDSDAARELDALLADIA
                     ALSPMISRAAGNDPRTPSFLIRRTGSDVQVGFAAIPLGHEFTSLVLALLQVGGHPVKI
                     DAALAAQVRALPGDYAFETYMSLHCQSCPDTIQALNAMSVLNPRIKHVAIDGALFQAE
                     VEAREILSVPTIYLNGQPFDAGRMSIEQIVARLDTGAAARAAAALHDVAPYDVLVVGG
                     GPAGAAAAIYAARKGIRTGLLAERFGGQVLDTMAIENFPSVEYTEGPKLAASLEAHVR
                     SYGVELVTSQRAKALQPAAAEGGMVGIELDSGATLRAKTVVLAPGARWRQTGVPGEAE
                     YRNKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFDGRLRADE
                     VLQRKLYSLPNASVHVNAQTTEFVGDGSKLQALRYTDRGTGASHQVEVAGVFVQIGLL
                     PNSEWLQGAVQLSPRGEIVIDDRGHTDLHGVFAAGDATTEPYKQIVVAMGAGSTAALS
                     AFDFLIRHSTPADAPAANAA"
BASE COUNT          762 a         1919 c         2107 g          891 t
ORIGIN      
        1 gcgcgctcgg cgtgtagccg cggcggcgga tgccctgcag ggtcggcatg cgcggatcgt
       61 cccagccgtg cacgcggccg gattcgacca gctgcagcag cttgcgcttg ctcatcacca
      121 gccagttgaa gttgagccgg gagaattcga tctggcgcgg cttggcggct tcgaacggca
      181 ggcccctgtc ggtcagcggc tgcaccagtt ccggcgagtg ggccaggtcc accttgtcca
      241 cgcaccagtc gtacagcggg cggtggtcct cgaactccag cgtgcacagc gaatgggtga
      301 tgccttccac cgcatcgctc agggaatgcg cgtagtcgta catcgggtag atcggccatt
      361 cgctgccggt gttctggtgc tcgacgtgct tgatccggta cagcgccggg tcgcgcaggt
      421 tgatgttgcc gctggccatg tcgatcttcg cgcgcagggt gcgcgcgccg tccacgaact
      481 cgcccgcgcg catccgccgg aacaggtcga ggttctcttc cggcgaacgc tcgcggtacg
      541 gcgaattgcg gcccggctcg gtcaggctgc cgcggtaggc gcgcacgtca tcggcggaga
      601 ggtcgcagac gaacgcatcg ccctggcgga tcagcttctc ggcggccagg tagaacacct
      661 cgaaatagtc ggaggcgtgg cgcagcgaat gccagtcgaa gcccagccag cgcacgtcgt
      721 cctggatcgc gcgcacgtac tcggggtctt ccttggccgg gttggtgtcg tccaggcgca
      781 ggttgcagtc gccgccgaac tcggccgcga tgccgaagtc caggcagatc gccttggcgt
      841 ggccgatgtg caggtagccg ttcggctccg gcgggaagcg cgtgcggatc gcggcgtgcc
      901 tgccgctggc aaggtcctcg cggacgatgc tgcggatgaa gtcccttttc tccggcgtgt
      961 tgtcgtcggc ggcgagggcg gggttggaag cggagtcgga cacgtgggga cgcttgcagg
     1021 aacggacccg cattctaacg gacgcgcggt ggcggccgcg gtatggcagc atcgccgcat
     1081 cccccgcctg aagcccgcga tgcgcgtcgt ctacgaagcc gccaatgtga tcgatgcgca
     1141 cctggtgcgg caggcgctgg agcatgccgg cctgccggca tgggtgcgcg gggaggcgct
     1201 gaccggcggc atgggcgagc tgggcgtgtt cggcctggtc gcggtgatgg tgccggacga
     1261 ctgcgtgccg gccgcgcgcg aggtggtgga agcgctgcag ctgggcgagc cgctgcaggc
     1321 actggacgac gaagacgacc ttcccggcac cgtgccggca tgaccgcggc ggcgcgcgcg
     1381 cgcgaggcgg ccatcgcgca gttgctggtc ggcgccgcga tcatcggcac caacggcttg
     1441 atggtgcggc tggcggggat gccaccgacc gcggtcgcgt tctggcggat gctgctggcc
     1501 gggctgatgc tgggcgcgct ggtgctggcg cggcatggct ggcaaccgct gtcgcgccgg
     1561 gcctggtggt ggtgcgcggt gccggcgctg gcgttcgcca tcgacctgtg gctgtggcac
     1621 cggagcatcc tgctggtcgg gcccggcctt gcgaccctgc tggccaatgc gcaggtgttc
     1681 ttcatggccc tggccggcgt gctgctgttc cgcgaacccc tcggactgcg cttcgcctgc
     1741 ggcgtgctgc tggcgttctt cgggctgtgg ctgatgctcg gcggcgactg ggccagcctg
     1801 ccggcggact accgatgggg cgtgtggctg gggctggcga ccgggcttgc gtacgcggtc
     1861 tacaacatcg gcatcaagcg ctcgcagggc gtggcggcgg ccgccaaggc gcgcgcgccg
     1921 gtggagcagg tgctgtgcat cgccgcgttc ggcagcgcgc tgtgcctggg gctgatgggg
     1981 tgggccgagg gcagcgcgtt caccccgccc gacctgcgtg cctggggcgt cttgctggtg
     2041 ctggctgcag tcggccattg cctgagctgg gtgctgatct cgcgggcgat ggtggccctg
     2101 ccggtggcgc tggccgggct gttgctgctg gcgcagccga tcgtggccta cctgctggac
     2161 gtcgccctgt tcgacctgcc cacctcgccg cggcaatggc tggggctggt ggtcagcctg
     2221 gccgggatct tcgtggcggg gatgaagtcg cgcagcgtga ccgtcgccga cgagccttga
     2281 ccgccgcgtg cgtcactccg gcgtgatcga acacgcgtaa accagttcgc aggcgccacc
     2341 gccgctgtcg tcgcgggtca gcgaactgcg ccagcgccag tcttcgccct cgacctgcac
     2401 gagccagccg tcgatgcgca cgcggtcgcc gcgttcgatc cggcgcagcg cggcggcgac
     2461 cgcgtcgtcg gccgggatca tgtgcatgtt ggcgctgctg cggatgatct cctgcagcgg
     2521 gatcggcggc gcgtcgctcc agcggtagcg gtaccagcgc gaggactggc tgatctgcag
     2581 gtgcgacagc accgcgtcct cggtcatccg gccccagccc agcgcgaggt cggtgggcga
     2641 gaattccgct tcgcggccca ggcggtagtc ctcgcggccg agcacgcgcg cctggatgct
     2701 gaaccccgcc aggggctgca acctcgcatc gccgatcgcc ggcagggcca tgccccccgg
     2761 caccggcgtc tgcagtggcg ggtcacccgg ctggatgcgg ggaggcagtg ggcaaccacg
     2821 cgtggccgca ctgccggcag cagtcgtggc gggcgtggtg gccgggcgcg tcgcctgcca
     2881 ccagaccagc acgcagacca gggtcaacac ggcgagcgaa gtacgcgcac ccggcatcga
     2941 aaaacgcccg cgatcaaagt gccggcaatt gtgcccgtgc cagcgggccg atggcatcgg
     3001 ccagggtgtg caggaagccg tccagcgccg aagacttgcg ccagaccagc gcgatcctgc
     3061 ggctcggggc gggcttgcgg aacggaacca cgcggatgtt cggcggctgc gcgaccggcg
     3121 catgcaccga cagcgccggc agcagggtca cgccaacgcc tgcggccacc atctgccgca
     3181 gggtctccag gctggtggcg cggaagccgg acttctcgtt ggcgccggac aaccggcaca
     3241 cgtccagcgc ctggtcgcgc aggcagtggc cgtcctccag cagcagcagg gtctcgtcgg
     3301 acagttgcgc catcgagacc gaggcccgcg ccgccagcgg gtgccgctgc ggcgccgcga
     3361 acacgaacgg ctcctcgaac agcagctccg cgtgcaactg ctcgtcgtgc accggcaatg
     3421 ccagcagcgc ggcgtccagc ttgccgtcgc gcaggcgctg cagcagcacg tcgctcttct
     3481 cctccaccag cagcagttcc agttgcggga agcgctcgcg cagctgcggc acgatgtgcg
     3541 gcagcaggta cgggccgagg gtcgggaaca cgccaagccg catggagccg gattccgggt
     3601 cccggctgcg ccgcgccgac tccttcatct gctcgacctc ggccacgatc cgacgcgcgc
     3661 gcagcaccac gtcctgcccg gcggcggtca gcatcacctt gcgcggcgcg cgttcgacca
     3721 gcgccacccc gagttcgtcc tccagcttgc ggatctgggt cgacagcgtg ggctggctga
     3781 cgaagcaggc gtcggcggcc ttgccgaagt ggcgcaggtc ggccagcgcg acgaggtatt
     3841 tcaggtcgcg caggttcatt cgtgcaggtc cggtccgagg tcaggccgca ttcgccgccg
     3901 gcgcatccgc cggggtcgag tggcggatca ggaaatcgaa ggccgatagc gccgcggtgg
     3961 agccggcgcc catcgccacc acgatctgct tgtacggctc ggtggtggcg tcgccggcgg
     4021 cgaacacgcc gtgcaggtcg gtgtggccgc ggtcgtcgat cacgatctcg ccgcgtgggc
     4081 tgagctgcac cgcgccctgc agccattcgc tgttgggcag caggccgatc tgcacgaaca
     4141 cgccggcgac ctcgacctgg tggctggcgc cggtgccacg gtcggtatag cgcagcgcct
     4201 gcagcttgct gccatcgccg acgaattcgg tggtctgcgc gttcacgtgc acgctggcgt
     4261 tgggcaggct gtaaagcttg cgctgcagca cctcgtcggc gcgcagcctg ccgtcgaact
     4321 cgagcagggt gacgtgctcg acgatgccgg ccaggtcgat cgccgcctcc accccggaat
     4381 tgccgccgcc gatcaccgcc acccgcttgc ccttgaacag cgggccatcg cagtgcgggc
     4441 agtaggtgac gcccttgttg cggtattcgg cctcgccggg cacgccggtc tgccgccagc
     4501 gcgcgcccgg ggcgagcacc acggtcttcg cgcgcagggt ggcgccgctg tcgagttcga
     4561 tgccgaccat cccgccctcg gccgccgccg gctgcagggc cttggcccgc tgcgaggtca
     4621 ccagttccac gccgtagctg cgcacgtgcg cctccagcga cgccgccagc ttcgggcctt
     4681 cggtgtattc gaccgagggg aagttctcga tcgccatggt atccagcacc tggccgccga
     4741 aacgctcggc cagcaggccg gtgcggatgc ccttgcgcgc ggcgtagatc gccgcggcgg
     4801 caccggccgg gccgccgccg acaaccagca cgtcgtaggg cgcgacatcg tgcagcgctg
     4861 cggccgcgcg cgcggcggcg ccggtatcca gcctggccac gatctgctcg atgctcatgc
     4921 ggccggcatc gaacggttgc ccgttgaggt agatcgtcgg caccgacagg atctcgcgcg
     4981 cttcgacttc ggcctggaac agcgcgccgt cgatcgcgac atgcttgatc cgcgggttca
     5041 gcacgctcat cgcattgagc gcctggatgg tgtccgggca gctctggcaa tgcagcgaca
     5101 tgtaggtttc gaaggcgtag tcgccgggca gcgcgcgcac ctgcgcggcc agcgccgcgt
     5161 cgatcttcac cggatgcccg cccacctgca gcagggccag caccagcgag gtgaattcgt
     5221 ggcccagcgg gatcgcggcg aagccgacct gcacgtcgct gccggtgcgg cggatcagga
     5281 acgagggcgt gcgcgggtca ttgccggccg cgcgcgaaat catcggcgac agcgcggcga
     5341 tgtcggcaag caacgcgtcc agttcgcggg cggcgtcgga gtcgtcaagc gaggccacca
     5401 gttcgatcgg ctgctgcagc ttctcgaggt aggcggcgag ctgggtcttc aggtcctggt
     5461 cgagcatggg gatcctcggg caaggctggg cggcgcgcgg cgcgcggccg gatgcgggaa
     5521 agggctgcgg ccaaccgccc gctgggggct ggccggagcc ctctccccgt gacggggaga
     5581 ggggcgggct gcgcgcgacg gacaggaacg ccgcgtgcag cggtggtcag atcttgccga
     5641 ccaggtccag cgacggcttg atggtcttgg cgccgtcgt
//
LOCUS       DAORUK010000137         5584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_17449_length_5584_cov_18.625430, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000137 DAORUK010000000
VERSION     DAORUK010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17449_length_5584_cov_18.625430"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PK818_08665"
     CDS             complement(<1..431)
                     /locus_tag="PK818_08665"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009606259.1"
                     /note="penicillin-binding protein 5; removes C-terminal
                     D-alanyl residues from sugar-peptide cell wall precursors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HQY82839.1"
                     /translation="MRLRSLLFVALATAGLAFAQTPVPAPAAKPAALSDNLPIPDAPA
                     PATSKAWLVMDYATGEVLAGENVDAAVDPASITKVMTSYVIAAEMAAGKVKPTDQVMM
                     SENAWRSGGAGTDGSYSGFEVNQAAPLVEMEKGMVVQSGNDA"
     gene            complement(540..1817)
                     /locus_tag="PK818_08670"
     CDS             complement(540..1817)
                     /locus_tag="PK818_08670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HQY82840.1"
                     /translation="MRAHRLALALLPLALAACATTGGTATPSKATDHHRKPVDATPAE
                     GIGPSPRRVSPYAPAQEDPTKRGDYVAGGLFRPAESDAVPDYIPDVDAIPEPEVRAEP
                     RARSGNRDYAVLGKRYKVLDSAHGYMEQGGASYYGNKFHGRRTSSGEVYDMYAFTAAH
                     KSLPLPSYARVTNLDNGKSVVVRVNDRGPFHSNRIIDLSYAAAVKLGYREKGVARVEV
                     RALTPGASSELAVAPAAADVSAMDRLVGTLPEALAAAGPGQPVAAPPKADAGYRFDMQ
                     QDGKVMTADEFDAWMTSRRVRVATGKPGTPDPAVAAATAAGDGSPQPAASPPPSPVNA
                     AALVAASAPPAGGSAEQVTLQVASFANQQNAQHALAMLQGAAIGRAQLFDTDVNGKKV
                     WRLRIGPVDVATAPELAARVVGLGFGQPQRVRD"
     gene            complement(1817..2932)
                     /gene="mltB"
                     /locus_tag="PK818_08675"
     CDS             complement(1817..2932)
                     /gene="mltB"
                     /locus_tag="PK818_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005911174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HQY82841.1"
                     /translation="MIRRALLPSLAALALAACAVPPPKPMPQPSAPPVPPPPVAPTPP
                     PVVFGDVSAARAEFVRSTSQRFGIPAAEIEAVLAKAQLREGIVKAMARPAEAKPWRDY
                     RPIFIQPRRIDGGRAFLAEHRAALQRAEDKYGVPKEVVTAILGVETSYGGNKGSHLVV
                     DALYTLAFAYPRSGDPAKVDRENKREAFFRDELAQLFALGKEQGFDIATLKGSYAGAM
                     GWGQFMPSSYRDYAVDGDGDGRVDLFDNLDDVFASVANYFAKKGKWQANGPVMERARR
                     DAGAADFANPGNAVTLDQGLAGLAAKGYRPAAMRFIHTENGPPVTLVTLEGAAGPEYW
                     IVYNNFKAITTYNISRLYATAVYQLAEAIAGRDPDPQ"
     gene            complement(2994..4121)
                     /gene="rodA"
                     /locus_tag="PK818_08680"
     CDS             complement(2994..4121)
                     /gene="rodA"
                     /locus_tag="PK818_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HQY82842.1"
                     /translation="MNIILRYLADLLLRLLRSIDLPLLAALLALMGIGLATLYSASNE
                     SSRMLATQGTYFGIGLGMLWLVSRIPAHVLKQLTPPIYVASLLPLVLVLFIGSGKYGN
                     HWINLGVFNFQPSELAKLTLPMMLAWHLDRNRLPPALSVLPMALLIIALPVGLILLQK
                     DLGTAVLVLGSGVFVLFLAGLSWWWFAVAGTVGISGFSLAMFAPIAWFSFLRPYQQDR
                     ILTFRDPENDPMGAGWNILQSKIAIGGGGLTGKGWTEGTQSHLDYLPEHTTDFVFSVL
                     SEEFGWLGVALVLALYLAVVGRCLWIAKESRDGYSRLLAGALGLSLFAYVLVNGAMVS
                     GLLPVVGVPMPLLSRGGTSSIILLASFGVVMALGARRAVHR"
     gene            complement(4223..5584)
                     /gene="mrdA"
                     /locus_tag="PK818_08685"
                     /pseudo
     CDS             complement(4223..5584)
                     /gene="mrdA"
                     /locus_tag="PK818_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019303045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
BASE COUNT          828 a         1968 c         1950 g          838 t
ORIGIN      
        1 gcatcgttgc cggactgcac caccatgccc ttttccatct cgaccagcgg cgcggcctgg
       61 ttgacctcga agccgctgta gctgccatcg gtgccggcgc caccgctgcg ccaggcattc
      121 tcgctcatca tcacctggtc ggtcggcttg accttgccgg cggccatttc ggcggcgatc
      181 acgtagctgg tcatgacctt ggtgatgctg gccggatcca ccgcggcatc gacgttttcg
      241 cccgccagca cctcgccggt ggcgtaatcc atcaccagcc aggccttgga ggtggccggc
      301 gcgggtgcgt cggggatcgg caggttgtcg ctgagcgcgg cgggcttcgc cgccggcgcg
      361 ggcaccgggg tctgggcgaa ggcgaggccg gcggtggcca gggcgacgaa cagcagggaa
      421 cgcagacgca tcaacaacac tcctcaaggc cgcgcaggcg cggcgcgaaa cggttcaatc
      481 ggccatttta agccgcaatc gcgggccgga cgccgtgacg tgccgccgaa agccaggctt
      541 cagtcgcgca cgcgctgcgg ttgcccgaag cccagaccga cgacccgggc cgcaagttcc
      601 ggcgccgtgg ccacgtccac cggcccgatc cgcaggcgcc agaccttctt gccattgacg
      661 tcggtgtcga acaactgggc gcggccgatg gcggcaccct gcagcatcgc cagcgcgtgc
      721 tgcgcgttct gctggttggc gaagctggcc acctgcaggg tcacctgctc cgccgaaccg
      781 cctgctggtg gcgcactcgc ggcgaccagc gccgccgcat tcaccggcga aggcgggggc
      841 gatgcagccg gctggggaga cccgtcgcct gccgccgtcg cggctgcaac cgccgggtcc
      901 ggcgtgcccg gcttgccggt ggccacccgc acccgccgcg acgtcatcca cgcgtcgaat
      961 tcgtcggcag tcatcacctt gccgtcctgc tgcatgtcga agcggtagcc ggcgtcggcc
     1021 ttcggcggcg ccgccaccgg ctggcccggg ccggccgcgg ccagcgcctc cggcagcgta
     1081 ccgaccagcc ggtccatcgc gctcacatcg gccgccgccg gtgcgaccgc cagctcgctc
     1141 gacgcgcccg gggtcagcgc gcggacctcg acccgggcca cgcccttttc ccgatagccc
     1201 agcttcaccg cggcggcgta gctgaggtcg atgatccggt tggaatggaa cggcccgcga
     1261 tcgttgacgc gcaccaccac cgacttgccg ttgtccaggt tggtgacccg cgcgtagctc
     1321 ggcagcggca gcgacttgtg cgcggcggtg aacgcgtaca tgtcgtagac ctcgccgctg
     1381 gaggtgcggc ggccgtggaa cttgttgccg tagtaggacg cgccgccctg ctccatgtag
     1441 ccatgcgcgc tatcgagcac cttgtagcgc ttgcccagca ccgcgtagtc gcggttgccg
     1501 ctgcgcgctc gcggctccgc gcggacttcg ggttccggga tcgcatccac gtccgggatg
     1561 tagtccggca ccgcatccga ttcggccggg cggaacagcc caccagccac gtaatcgccg
     1621 cgcttggtcg ggtcttcctg cgccggcgca tatggcgaga cccggcgcgg cgacggcccg
     1681 ataccctcgg cgggcgtggc atcgacgggc ttgcgatggt gatccgtggc cttcgatgga
     1741 gtagcggtgc cacctgtggt ggcacaagcg gccagcgcga gcggcaacag cgccagcgcg
     1801 agtcggtgcg cccgcattac tgcggatccg ggtcccgccc ggcgatcgct tcggccagct
     1861 ggtagaccgc ggtcgcgtac aaccgcgaga tgttgtaggt ggtgatcgcc ttgaagttgt
     1921 tgtagacgat ccagtattcg ggaccggcgg cgccttccag ggtgaccagg gtcaccggcg
     1981 gcccgttttc ggtgtggatg aagcgcatcg ccgcaggccg gtagcccttc gcggcgagcc
     2041 cggccaggcc ctggtccagg gtcaccgcgt tgcccgggtt ggcgaagtcg gcggcgccgg
     2101 cgtcgcgcct ggcgcgctcc atcaccgggc cgttcgcctg ccacttgccc ttcttcgcga
     2161 agtagttggc gaccgaggcg aacacgtcgt cgaggttgtc gaacaggtcg acgcggccgt
     2221 cgccatcgcc gtccaccgcg tagtcgcgat agctggacgg catgaactgg ccccagccca
     2281 tcgcgccggc atagctgccc ttgagcgtgg cgatgtcgaa gccttgttcc ttgccgagcg
     2341 cgaacagctg cgcgagttca tcgcggaaga acgcctcgcg cttgttttcg cgatcaacct
     2401 tggcggggtc gccgctgcgc ggataggcga aggcgagcgt gtacagggca tcgaccacca
     2461 ggtgactgcc cttgttgccg ccgtagctgg tttccacgcc gaggatcgca gtcacgactt
     2521 ccttcggcac gccgtatttg tcctcggcgc gctgcagcgc tgcgcgatgc tcggcgagga
     2581 aggcacggcc gccgtcgatg cgcctgggct ggatgaagat cggccggtag tcgcgccagg
     2641 gcttggcttc cgccggccgc gccatcgcct tgacgatgcc ctcgcgcagt tgcgccttgg
     2701 ccagcaccgc ctcgatctcc gcggcgggaa tcccgaagcg ctgcgaggtg ctgcgcacga
     2761 actcggcgcg cgcggcgctc acgtcgccaa acacgaccgg tggtggcgtc ggcgccaccg
     2821 gcggcggcgg aacgggcggt gccgacggct gcggcatcgg cttcggcggc gggacggcgc
     2881 aggcggcaag cgcaagcgcg gcgaggctgg ggagcagggc gcgtcggatc atcggcggga
     2941 gggtagcacg ggcctccgcg cacgccggaa ccggcgattc agcttgcgcg cattcagcga
     3001 tggaccgcgc gccttgcgcc cagcgccatc accacgccga agctggccag caggatgatc
     3061 gacgaggtgc cgccgcggct gagcaggggc atcggcaccc cgaccaccgg cagcaggccg
     3121 gagaccatcg cgccgttgac cagcacgtag gcgaacagcg acagccccag cgcgccggcc
     3181 agcagccgcg agtagccatc gcgcgattcc ttcgcgatcc acaggcagcg cccgaccacc
     3241 gccaggtaca gcgccagcac cagcgccacc cccagccagc cgaattcctc gctgagcacg
     3301 gagaacacga agtcggtggt gtgttccggc aggtagtcca ggtgcgactg ggtgccttcg
     3361 gtccagccct tgccggtcag gccgccgcca ccgatcgcga tcttgctttg caggatgttc
     3421 cagccggcgc ccatcgggtc gttctcggga tcgcggaagg tcaggatgcg gtcctgctgg
     3481 tacgggcgca ggaacgagaa ccacgcgatc ggcgcgaaca tcgcaagcga gaaaccgctg
     3541 atcccgacgg tgccggcgac cgcgaaccac caccacgaca acccggccag gaacagcacg
     3601 aacacgccgc tgcccagcac cagcaccgcc gtgcccaggt ccttctgcag caggatcagg
     3661 cccaccggca gggcgatgat cagcaacgcc atcggcagca ccgacagcgc cggcggcagc
     3721 cggttccggt ccaggtgcca ggccagcatc atcggcaggg tcagcttggc cagctccgag
     3781 ggctggaaat tgaacacgcc gaggttgatc cagtggttgc cgtacttgcc gctgccgatg
     3841 aacagcacca gcaccagcgg cagcagcgag gccacgtaga tcggcggggt gagctgcttg
     3901 agcacgtgcg cggggatccg cgaaaccagc cacaacatgc ccaggccaat cccgaaatag
     3961 gtgccctggg tggccagcat gcggctggat tcgttgctcg cgctgtacag ggtggccagg
     4021 ccgatcccca tcagcgccag cagcgcggcc agcagcggca ggtcgatgct gcgcagcagg
     4081 cgcagcagca ggtcggcgag gtagcgcagg atgatgttca tcgcgccggt tccgttgcca
     4141 cggggggcac cgcctgcgga tccgcgtccg cgctggcggc gaccgccgga tcgccggcgg
     4201 cggcttgcgc aggcgcgggc accggcatct tgcccagcag ccacgcatcc agcaccttgc
     4261 gcgcgaccgg tgcggcactg gacgcgccat agccgccgcc ctcgatcgcc accgcgatgg
     4321 cgatggtcgg cgcatccgcc ggcgcatagg cgatgaacag gccacggtgg cgcaggtgca
     4381 tcggcagcga gcgcgggttg accgcggcgt tgccgcgccg gctgaccacc tgcgcggtgc
     4441 cggtcttgct ggcgatcagg taggccaggc cgggccggat gttggtggcg gtaccgggac
     4501 cgtgcacggt ggcgatcatg ccctcgcgca ccgcctgcag gtgcgcgggg ttgtcgctga
     4561 tccgggtcgg cggcaccagc tcgacctgcg tccactcggc ggcgaacccg gcgcgcatgt
     4621 ccttgaccag gtgcggccgc cgcagccagc catcggccag cgccgcggtg gcctgcgcca
     4681 tctgcatcgg cgtcaccttc cagaagccct ggccgatgcc ggagatcacc gtgtcgcccg
     4741 gataccagcg gccctgcgcg ggcgcgttct tcgccttcca ctccggcgag ggcaggatcc
     4801 cggcgatctc gccggacagg tcgaccccgg tcggcttgcc gaacccgtag cggtccatgt
     4861 actcgtcgaa gcgggcgatg cccatgtcca gcgccagccg gtagtagtag gtgttgaccg
     4921 attcgtagat cgacttgcgc aggtcggtcc agccatgccc gccacgatgc gagtcgccat
     4981 agccgcggcg ctggccgccg acatggaaca tgccggtcga cagggtcttg tcctcggccc
     5041 tgcgcacccc gctgtcgagg ccggcaagcg cgagcaacgg cttgatcgtg gaccccggcg
     5101 ccaccccgcc gagcaccgcg cggttgaact gcgggcgcga gggattgtcg ttgagcgccc
     5161 tgtaatcggc atggctgatg ccgttgacga acaggttggt gtcgtaggcc ggcaggctga
     5221 ccatcgccag gatctcgccg ctgcgcggat ccaccgccac cgcggtgcct tccagttcgc
     5281 cgaacgcgtc caccatcgcg cgctgcaggt cgaggtcgat cgacagccgc aggtcggtgc
     5341 ccggggtcgc cggcacggtg tccagcgcgc gcacggtgcg gccgtcgaca ttggtctcca
     5401 gccgcctgta gccgggcttg ccgcgcagct gcccgtcgta atagcgctcc agcccggtct
     5461 tgccggtatg cgagtacgca ctgccggcgg gaccgagcgc ggcctggtcc ttctcgtcga
     5521 tgcggccgac gtagccgacc acgtgcgcga acagcggtcc gtgcggatac acccggccga
     5581 ggta
//
LOCUS       DAORUK010000138         5535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_17671_length_5535_cov_19.763686, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000138 DAORUK010000000
VERSION     DAORUK010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17671_length_5535_cov_19.763686"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PK818_08690"
     CDS             complement(<1..586)
                     /locus_tag="PK818_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HQY82843.1"
                     /translation="MNRPAARLLAVSKTRSTGEVAALADAGQQAFGENYVQEALPKIE
                     ALRGRGLEWHLIGHLQSNKADLAARHFDWVQSLDRAKLVTALARHRPAGAPPLNVLVQ
                     VNIDDETSKHGCLPGDVPALAKAIEAEPSLRLRGLMAIPAPHADMTRRRDAFRAMHAL
                     FEALQAGHAGIDTLSMGMSEDFVPAIEEGATMVRI"
     gene            775..1812
                     /locus_tag="PK818_08695"
     CDS             775..1812
                     /locus_tag="PK818_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HQY82844.1"
                     /translation="MDIAELLAFSVKNRASDLHLSAGLPPMIRVDGDVRRINIPAFDH
                     KQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPGLARFRVNAFNQNRGAGAVFRTIPSE
                     VLTLEDLGCPRIFKELIEQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYGHILSVED
                     PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAA
                     ETGHLVFATLHTSSAAKTIDRIIDVFPAGEKPMVRSMLSESLRAVIAQALLKKTGGGR
                     TAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQNAGMQTLDQHLQDLVKRGLVSRQQA
                     REYAKDKRLFE"
     gene            1859..2989
                     /locus_tag="PK818_08700"
     CDS             1859..2989
                     /locus_tag="PK818_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003489921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HQY82845.1"
                     /translation="MSSIDFTSFLKLMAHQKASDLFITAGMPPSMKVHGKISPITQAP
                     LTPQQSRDLVLNVMTPQQREEFEKTHECNFAIGVSGVGRFRVSCFYQRNQVGMVLRRI
                     ETRIPTVEELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNMNSTGHIIT
                     IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAI
                     AFAETGHLVLCTLHANNANQAMDRVINFFPEDRRNQLLMDMSLNLKGVVAQQLIPTPD
                     GKARRVAMEILLGTPLVQDYIRDGEIHKLKEVMKESVQLGMKTFDQSLFELYQAGEIS
                     YEDALRFADSTNEVRLRIKLAQGGDASTLSQGLDGVEVAEAR"
     gene            3046..3612
                     /locus_tag="PK818_08705"
     CDS             3046..3612
                     /locus_tag="PK818_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002802054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HQY82846.1"
                     /translation="MSIEPSPLSNQLLVALPALHDPHFARSVALICQHDGDGAMGVVI
                     NRPSEYSLGEVLQQMGIASDSEALQSQVVLAGGPVHPERGFVLHDGDREWDSTLAVGD
                     GLYLTTSRDVLEALARGDGPAQAVVALGCAGWGAGQLEQELVEDSWLMVPAQRAVLFD
                     LPLDQRWQAAAGSIGVDLVNYASHSGHA"
     gene            3609..4088
                     /gene="ruvX"
                     /locus_tag="PK818_08710"
     CDS             3609..4088
                     /gene="ruvX"
                     /locus_tag="PK818_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HQY82847.1"
                     /translation="MSDVVDPQPAIRRDGIVLGFDVGARRIGVAIGSAFASGARALAV
                     VDVHAAGPDWNRIEALRREWRPDGLVVGDPMGLDGGDQPVRKLAQAFARELRRRTGLP
                     VVMVDERNSSQDAARRFAADRAEGKRKRRDAEVLDAVAAAVIVDRWFASPQDATVIA"
     gene            4085..5029
                     /locus_tag="PK818_08715"
     CDS             4085..5029
                     /locus_tag="PK818_08715"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005913284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HQY82848.1"
                     /translation="MTGLFGTSGRMHHLLTLEHMPRAMLEHLLVRAQDLAADAEGGVA
                     RRHVLAGKAVCTLFFEPSTRTRSSFSLAAQRLGADLLNFDASTASTTKGEADLDTLKT
                     LEAMGVHAFVVRHKRDGAVAELAAAANPGTVLLNAGDGRSNHPTQGLLDMLTLRQAKG
                     EDFAKLRIVIVGDVKHSRVARSDLHALRALGCGEIRVCGPAALLPEDGTLDGCVVSHD
                     FDAAIEGVDAAMMLRLQRERMAEGLVASLEDYHRDFGLDAARLRRAAPEAIVMHPGPM
                     NRGIEITDEVADGPQSRVLRQVANGVAVRMAVLEALLG"
     gene            complement(5026..>5535)
                     /locus_tag="PK818_08720"
     CDS             complement(5026..>5535)
                     /locus_tag="PK818_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014502484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HQY82849.1"
                     /translation="FRNRLIWLGVNLCTAFLAASVVDRFSGSIEQLAALAVLMPIVAG
                     MGGNAGTQVLALMIRGLALGQIGASNVPTLVWKEVRVALINGLALGAVLGLVVLAWFR
                     NPALAAVIASALTINLCFAALGGVFVPVTLKRLGFDPALAGGVILTTITDVMGFLTFL
                     GLATLVLLG"
BASE COUNT          931 a         1888 c         1910 g          806 t
ORIGIN      
        1 cgatgcgcac catggtcgca ccttcctcga tggcgggcac gaagtcctcg ctcatcccca
       61 tcgacagggt gtcgatcccg gcatggcccg cctgcagagc ttcgaacagg gcgtgcatgg
      121 cgcggaatgc gtcgcgccgg cgggtcatgt cggcgtgcgg cgcggggatc gccatcagtc
      181 cgcgcaggcg caaggacggc tcggcttcga tggccttggc cagtgccggt acgtcgcccg
      241 gcaggcagcc gtgcttgctg gtttcatcgt cgatgttcac ctgcaccagc acgttcaagg
      301 gcggtgcgcc ggcgggccgg tggcgggcca gtgcggtcac cagcttggcg cggtccagcg
      361 attgcaccca gtcgaaatgg cgcgccgcca ggtccgcctt gttcgattgc aggtggccga
      421 tcaggtgcca ttccagcccg cgtccccgca gcgcttcgat cttcggcaat gcttcctgca
      481 cgtagttctc gccaaaggcc tgctggccgg catccgccag cgcggcgacc tcgcccgtgc
      541 tgcgcgtttt cgagaccgcc agcaagcggg ccgctggccg gttcatgcgc gtggccacgt
      601 tctcgatagg caacaggact gcgccaagtg cgctgctggg gtcgggcaag gagggctcgg
      661 gcacggcggt tgacactccg gggcgggcgc tcatactgcc cgcggggtgc gtgcgacgcc
      721 actcgggaag gtggccgcag gcgcgacatc acaacgaggg gcggggagca cctaatggat
      781 attgccgagc tgctggcgtt ttcggtcaag aacagggcct cggacctgca cctttccgcg
      841 gggctgccgc cgatgatccg cgtcgatggc gacgtgcgcc gcatcaacat ccccgccttc
      901 gaccacaagc aggtgcacgc gctggtctac gacatcatgt cggacaagca gcgccgcgac
      961 tacgaggaat tcctcgaggt cgacttcagc ttcgagatcc cgggcctggc gcgcttccgc
     1021 gtcaacgcat tcaaccagaa ccgcggtgcc ggcgcggtgt tccgcaccat tccctcggaa
     1081 gtgctgaccc ttgaggacct gggctgcccg cgcatcttca aggaactgat cgagcagccg
     1141 cagggcctga tcctggtcac cggcccgacc ggctccggca agtcgaccac gctcgcggcg
     1201 atgatcgacc acatcaacaa gaacgaatac ggccacatcc tctcggtcga ggatccgatc
     1261 gagttcgtgc acacctcgca gaagtgcctg atcaaccagc gcgaggtcca tcgcgacacc
     1321 cacggcttca acgaggccct gcgctcggcc ctgcgcgagg acccggacat catcctggtc
     1381 ggcgagttgc gcgacctgga aaccatccgc ctggcgctga ccgccgcgga gacgggccac
     1441 ctggtgttcg ccaccctgca tacctccagc gcggccaaga ccatcgaccg catcatcgac
     1501 gtgttccccg ccggcgagaa gccgatggtg cgctcgatgc tgtccgaatc gctgcgtgcg
     1561 gtgatcgccc aggcgctctt gaagaagacc ggcggcgggc gcaccgcggc gtgggaaatc
     1621 atggtcggca cccccgccat ccgcaacctg atccgcgagg acaaggtcgc gcagatgtac
     1681 tcgtcgatcc agaccggcca gaacgccggc atgcagaccc tggaccagca ccttcaggac
     1741 ctggtcaagc gcggcctggt gtcgcgccag caggcccgcg agtacgccaa ggacaagcgc
     1801 ctgttcgaat gaaccgggca atcacggatc gacgcgccac cggaccggag ccacgcagat
     1861 gagcagcatc gacttcacct cgttcctcaa gctgatggcg caccagaagg cgtccgactt
     1921 gttcatcacc gccggcatgc cgccgtcgat gaaggtgcac ggcaagatca gcccgatcac
     1981 ccaggcgccg ctgaccccgc agcagtcgcg cgacctggtg ctgaacgtga tgacgccgca
     2041 gcagcgcgag gagttcgaga agacccacga gtgcaacttc gcgatcggcg tgtccggggt
     2101 cggtcgcttc cgcgtgagct gcttctacca gcgcaaccag gtgggcatgg tgctgcgccg
     2161 gatcgagacc cgcatcccga ccgtggaaga gctcaacctg ccgccggtca tcaagaccct
     2221 ggcgatgacc aagcgcggca tcatcatctt cgtcggcgcc accggcaccg gcaagtcgac
     2281 ctcgctggcg gcgatgatcg gctaccgcaa catgaactcc accgggcaca tcatcaccat
     2341 cgaggacccg atcgaattcg tgcacaagca cgagggctgc atcatcaccc agcgcgaggt
     2401 cgggatcgac accgacagct gggagaacgc gctgaagaac accctgcgcc aggccccgga
     2461 cgtgatcatg atcggcgagg tgcgcacccg cgagggcatg gaccacgcga tcgcgttcgc
     2521 cgaaaccggc cacctggtgc tgtgcacgct gcacgccaac aacgccaacc aggcgatgga
     2581 ccgggtcatc aacttcttcc cggaagaccg ccgcaaccag ctgctgatgg acatgtcgct
     2641 gaacctcaag ggcgtggtcg cgcagcagct gatcccgacc ccggacggca aggcgcgccg
     2701 ggtcgccatg gagatcctgc tgggcacccc gctggtgcag gactacatcc gcgacggcga
     2761 gatccacaag ctgaaggagg tcatgaagga atccgtgcaa ctgggcatga agaccttcga
     2821 ccagagcctg ttcgagctgt accaggccgg cgagatcagc tacgaagacg cgctgcgctt
     2881 cgccgattcg accaacgagg tgcgcctgcg gatcaagctg gcgcagggcg gcgacgcgtc
     2941 gaccctgtcg caggggctgg atggcgtcga ggtcgcggaa gcgcgctgaa cgcgttggcc
     3001 tggcgccggc gtgcgccagc gggcatgcct cgctagaatt gcggcatgtc gatcgagcct
     3061 tcgccgctgt ccaaccaact gctggtggcg ctgcccgcgc tgcacgaccc gcatttcgcg
     3121 cgcagcgtcg cgctcatttg ccagcacgat ggtgacggcg cgatgggggt ggtgatcaac
     3181 cggccctccg aatactcgct gggcgaagta ctgcagcaga tgggcatcgc cagcgacagc
     3241 gaggccctgc agtcgcaggt cgtgctcgcc ggcggcccgg tgcatccgga gcgcggcttc
     3301 gtcctccacg acggcgaccg cgaatgggat tcgaccctgg cggtcggcga cggcctgtac
     3361 ctgaccacct cgcgcgacgt gctcgaggcg ctggcgcgcg gcgacggccc ggcgcaggcg
     3421 gtggtggcgc tgggctgcgc cggatggggc gcgggccagc tggaacagga actggtcgag
     3481 gacagctggt tgatggtgcc cgcgcagcgc gcggtgctgt tcgacctgcc gctggaccag
     3541 cgctggcagg cagcggccgg cagcatcggc gtggacctgg tcaattacgc cagccacagc
     3601 ggccacgcat gagcgacgtc gttgatccgc agcccgcgat ccgccgcgac ggcatcgtcc
     3661 tcggcttcga tgtcggcgcg cgccggatcg gggtggcgat cggcagtgcg ttcgccagcg
     3721 gcgcccgtgc gctcgcggtg gtcgacgtgc acgccgccgg cccggactgg aaccggatcg
     3781 aggcgctgcg ccgcgaatgg cggccggatg ggctggtggt cggcgatccg atggggctgg
     3841 atggcggcga ccagccggtc cgcaagctcg cgcaggcgtt cgcccgcgaa ttgcggcggc
     3901 gcaccggcct gccggtggtg atggtcgacg aacgcaacag ctcgcaggac gccgcgcgcc
     3961 gattcgccgc ggatcgcgcc gaaggcaagc gcaagcggcg cgatgccgag gtgctggacg
     4021 cggtggccgc ggcggtgatc gtcgatcgct ggttcgcgtc gccgcaggat gcaacggtga
     4081 tcgcatgacc ggcctgttcg gcacgagcgg caggatgcac cacctgctga ccctcgagca
     4141 catgccgcgg gcgatgctgg aacacctgct ggtgcgcgcg caggacctcg ccgccgatgc
     4201 cgagggcggg gtcgcgcggc gccacgtgct ggccggcaag gcggtgtgca cgctgttctt
     4261 cgagccgtcc acccgcaccc gctccagttt ttcgctggca gcgcagcggc ttggcgccga
     4321 cctgctcaat ttcgacgcct ccaccgcctc caccaccaag ggcgaggccg acctcgacac
     4381 cctgaagacg ctggaagcga tgggcgtgca cgcgttcgtg gtgcgccaca agcgcgacgg
     4441 cgcggtggcg gaactcgcgg cggcggccaa ccccggcacc gtgctgctga acgcgggtga
     4501 tggccgcagc aaccatccca cccagggcct gctggacatg ctgacgctgc gccaggccaa
     4561 gggcgaggat ttcgcgaaac tgaggatcgt gatcgtcggc gacgtgaagc attcgcgggt
     4621 cgcccgttcc gacctgcacg cgttgcgtgc gctcggctgc ggcgagatcc gcgtgtgcgg
     4681 cccggccgcg ctgttgccgg aagacggcac cctggatggc tgtgttgtca gccacgactt
     4741 cgacgcggcg atcgaaggcg tggatgcggc gatgatgctg cgcctgcagc gcgagcgcat
     4801 ggccgaaggc ctggtcgcct cgctcgagga ctaccaccgc gacttcgggc tggatgccgc
     4861 gcgcctgcgc cgcgccgcgc cggaggcgat cgtgatgcac cccgggccga tgaaccgcgg
     4921 catcgagatc accgacgaag tcgcggacgg cccgcagtcg cgggtgctgc ggcaggtcgc
     4981 gaacggcgtg gcggtgcgga tggcggtgct ggaggcgttg ctgggttagc ccagcagcac
     5041 cagcgtcgcc agcccgagga aggtcaggaa gcccatcacg tcggtgatgg tggtcaggat
     5101 cacgccgccg gccagcgccg ggtcgaagcc cagccgcttg agcgtgaccg gcacgaacac
     5161 gccgccgagc gcggcgaaac acaggttgat ggtcaatgcg ctggcgatca ccgcggccag
     5221 cgccgggttg cggaaccagg ccagcaccac caggccgagc acggcaccga gcgcgaggcc
     5281 gttgatcagc gccacccgca cttccttcca gaccagggtc ggcacgttgg acgcgccgat
     5341 ctggcccagc gccaggccgc ggatcatcag cgccagcacc tgggtgccgg cgttgccgcc
     5401 catcccggcc acgatcggca tcagcaccgc cagcgcggcg agctgttcga tggagccgga
     5461 gaaacggtcc accaccgagg ccgccaggaa cgcggtgcac aggttcacgc ccagccagat
     5521 caggcggttg cggaa
//
LOCUS       DAORUK010000139         5490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_17892_length_5490_cov_19.109292, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000139 DAORUK010000000
VERSION     DAORUK010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17892_length_5490_cov_19.109292"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3260)
                     /locus_tag="PK818_08725"
     CDS             complement(<1..3260)
                     /locus_tag="PK818_08725"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HQY82850.1"
                     /translation="MMSGWILLLVALAYVGLLFTVAWLGERRPQLAQGPRARATVYAL
                     ALAVYCSSWTFYGAVGTAARAGLGFLPIYLGPILLLLFGWRILERLVLASTRHRIVSI
                     ADFLSSRYGRARGLAAAVAVLAVIAAIPYFALQFKAVGMSVGVLSGQPLAAQWYADPA
                     LYVALLLAAFAILFGTRRIDATEHHRGMVLAIALESLVKLLAFVAIGALAILHLPGGG
                     GIGTRVAAGAGAFASATLPSGFLAQTLLAFTAIVCLPRQFHVAVVECQDPADLQPARW
                     LFIGYLVLVSAMVLPIALAGQALPGADGLAADSFVLALPLAHGHPWLALAAYIGGLSA
                     ATGMVIVASVALSTMVSNDLVLPLLLRRGVLSQRMAIERQVLWVRRVAILLLALVAFA
                     YHRHALGQQFLAQHGLLAFAAVAQFAPALIGGLYWRGASRAGAYAGLFSGAALWAWCL
                     LLPALLQGNGPSPDWLREGPLGLAWLRPESLFGLRGWDPLTHGVFWSLLGNVAAFVFV
                     SMRYRPLLQDQLAAAAYLDPYAHQPALAPGGWAGKVRGSELLTLAERILGEAHARRAF
                     QEYAGSLARPWDPEQPADRALVQHTERLLASALGAASARSTLTSVLRGSGMELGEVVS
                     LLDETSNALRYNREVLTTTLENIAQGVSVVDAQMRLVAWNRRYQQLFDYPDGMLYVGR
                     PVADLLRWNAARGEMGPGDAEAHIERRLEHLRAGTPHVFERVRANGQVIEMHGQPLPG
                     GGYVTSYSDISDYKRVEQALREVNETLEQRVELRTREAEAAQQSKTRFLAAISHDVLQ
                     PMNAARLFASAMRESDDPAEQARLATRVDASLRAAEELLDGLLDVSRLDAGVLQPQPA
                     DFDAAELLRELAAQYAPTAAQRGLALRVHASRAIPVRSDRHLLRRALQNFIGNALRYT
                     QAGGVLLAARQRRDGIALEVWDTGPGIPRAHLRQIFEEFHRFDQPGDRGERGLGLGLS
                     ICQRISHVLGHPLAVQSRVGRGSVFAIRVPRAARLPAPAPERDARPVQDDALHGLRVL
                     CLDNDREILAGMQALLARWGVQVAVAATVDEALAAMPTSPQVLLVDYHLHDRMDGL"
     gene            3343..4731
                     /locus_tag="PK818_08730"
     CDS             3343..4731
                     /locus_tag="PK818_08730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQY82851.1"
                     /translation="MDHAPETPPLALPQSRPWGRVFVRRVGLGIAGFALLVLLDWQPS
                     LGLLRGLLPGMVLKPLAALGFLLAGLVLANEARRVPWPAVSVLLSVAIAVAGAASLHA
                     DLAGVALASDRWFPDPEAAITSGIAGRMSPLTACGLLLLGLLGALRLDGACLRLRHGL
                     ALLVLGIAMFTLAAYGQQVGDAAFLPVPLLAAGLFLAAALAWLAVLPARGLLHVVHST
                     GIGGLVSRRLLLPALLLPGLLGVLARLAGSRAGWSAITVASIQALATGALLAWMIWWV
                     ATLLERLQRQRDEVRVLHDESHTDALTGLPNRRAFDEAIAVLGQGRRGHDHGGSLLLL
                     DLDRFKDYNDRFGHQAGDAVLRQVGALLRHAVRPSDLPARYGGEEFAVLLPEAGIEEA
                     MQVAERIVRAFRAVAWPGRAVTISVGAAALAGSESADALVGRADAALYAAKAAGRDRV
                     VQAAAPAQPAPA"
     gene            complement(4798..4962)
                     /gene="rpmG"
                     /locus_tag="PK818_08735"
     CDS             complement(4798..4962)
                     /gene="rpmG"
                     /locus_tag="PK818_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HQY82852.1"
                     /translation="MASKRDKIRLISSAGTGHFYTTDKNKKNTPAKMEVSKYDPVVRK
                     HVTYKEGKIK"
     gene            complement(4975..5211)
                     /gene="rpmB"
                     /locus_tag="PK818_08740"
     CDS             complement(4975..5211)
                     /gene="rpmB"
                     /locus_tag="PK818_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HQY82853.1"
                     /translation="MSKVCQVTGKRTVSGNNVSHSNRKTRRRFMPNLHERRFWVASEN
                     RWVKLRVSAAALRTIDKNGIDAILADLRKQGEKV"
BASE COUNT          754 a         1911 c         2029 g          796 t
ORIGIN      
        1 tcgaggccgt ccatgcggtc gtgcaggtgg tagtcgacca gcaacacctg cggcgaggtc
       61 ggcatcgccg ccagcgcctc gtccaccgtt gccgccaccg cgacctgcac gccccagcgc
      121 gccagcaggg cctgcatgcc ggcgaggatc tcgcggtcgt tgtccaggca cagcacgcgc
      181 agtccgtgca gcgcatcgtc ctgcaccggc cgtgcatcgc gttccggcgc cggcgcgggc
      241 aggcgggcgg cgcgcggcac ccggatcgcg aacacgctgc cgcggccgac ccgcgactgc
      301 accgccagcg gatgccccag cacgtgcgaa atgcgctggc agatcgacag gcccaggccc
      361 agcccgcgtt cgccacggtc gccgggttgg tcgaagcggt ggaactcctc gaagatctgc
      421 cgtaggtgcg cccgcgggat gccgggcccg gtgtcccaca cttccagcgc gatgccgtcg
      481 cggcgctggc gcgccgccag cagcacgccg ccggcctggg tgtagcgcag cgcattgccg
      541 atgaagttct gcagcgcgcg ccgcagcagg tggcggtcgc tgcgcaccgg gatcgcgcgc
      601 gacgcatgca cgcgcagggc aagcccgcgc tgggccgccg ttggcgcgta ttgcgcggcg
      661 agttcgcgca gcagttcggc ggcgtcgaaa tcggccggct gcggctgcag cacgcccgca
      721 tccagccggg agacgtcgag caggccgtcc agcagttctt ccgccgcgcg cagcgaggcg
      781 tccacgcggg tcgccaaccg cgcctgttcg gcggggtcgt cgctttcgcg catggcggag
      841 gcgaacaggc gcgcggcgtt catcggctgc aacacgtcat ggctgatcgc ggcgaggaag
      901 cgggtcttgc tctgctgcgc ggcctcggcc tcgcgggtgc gcagttcgac ccgctgctcc
      961 agggtctcgt tgacctcgcg cagcgcctgt tcgacgcgct tgtagtcgct gatgtcgctg
     1021 tagctggtga cgtaaccgcc gccgggcagc ggctgtccgt gcatttcgat gacctggccg
     1081 ttggcgcgca cgcgctcgaa cacgtgcggc gtgcccgcgc gcaggtgctc cagccggcgt
     1141 tcgatgtgcg cctcggcgtc gcccgggccc atctcgccgc gggcggcgtt ccagcgcagc
     1201 aggtcggcga ccggccggcc gacgtagagc atgccgtcgg ggtagtcgaa cagctgctgg
     1261 tagcggcggt tccaggcgac aagccgcatc tgcgcatcga ccacgctcac cccctgcgcg
     1321 atgttctcga gggtggtggt cagcacctcg cggttgtagc gcagggcgtt gctggtttcg
     1381 tccagcaggc tgaccacttc gcccagctcc atgcccgaac cgcgcagcac cgaggtcagg
     1441 gtgctgcgtg cggaggcggc gcccagcgcg gacgccagca ggcgctcggt gtgctgcacc
     1501 agcgcgcggt cggccggctg ttcgggatcc cacggccgcg ccaggctgcc ggcgtattcc
     1561 tggaaggccc gccgcgcatg cgcctcgccg aggatgcgtt cggccagggt cagcagttcg
     1621 ctgccgcgca ccttgccggc ccagccgccg ggcgccagcg ccggctggtg cgcgtaggga
     1681 tccaggtatg cggcggcggc cagctggtcc tgcagcagcg gccggtagcg catcgacacg
     1741 aacacgaacg cggccacgtt gcccagcagc gaccagaaca cgccgtgggt cagcggatcc
     1801 cagccgcgca gcccgaacag cgactccggg cgcagccagg ccaggcccag cgggccttcg
     1861 cgcagccagt cggggctggg tccattgccc tgcagcagcg ccggcagcag caggcaccag
     1921 gcccacaacg cggccccgct gaacaggcct gcataggcgc cggcacggct ggcgccgcgc
     1981 cagtacaacc cgccgatcag ggccggcgcg aattgcgcca ccgcggcgaa tgcaagcagg
     2041 ccgtgctggg caaggaactg ctggcccagc gcgtgccggt ggtaggcgaa tgcgaccaat
     2101 gccagcagca ggatcgccac ccggcgcacc cacagcacct ggcgttcgat cgccatccgc
     2161 tgcgaaagca ccccgcgccg cagcagcagc ggcagcacca ggtcgttgct gaccatggtc
     2221 gacagcgcca cgctggccac gatcaccatg ccggtggccg cggacaggcc gccgatgtag
     2281 gccgccagcg ccagccacgg gtggccgtgc gccagcggca gcgccagcac gaagctgtcc
     2341 gcggccagcc cgtccgcgcc gggcagcgcc tgcccggcca gcgcgatcgg cagcaccatc
     2401 gcgctgacca gcaccaggta gccgatgaac agccagcgcg ccggctgcag gtcggccggg
     2461 tcctggcact cgaccaccgc cacgtggaac tggcgcggca ggcacacgat cgcggtgaac
     2521 gcgagcaggg tctgggcgag gaagcccgat ggcagggtgg cgctggcgaa tgcgccggca
     2581 ccggccgcga cccgggtgcc gatgccgccc ccgcccggca ggtgcaggat tgccagcgcc
     2641 ccgatcgcca cgaacgccag cagcttgacc agcgattcca gcgcgatcgc cagcaccatc
     2701 ccgcggtggt gctcggtggc atcgatccgg cgggtgccga acaggatcgc gaaggccgcc
     2761 agcagcagcg ccacgtacag ggccgggtcg gcataccact gcgccgccag cggttgcccg
     2821 ctgagcacgc ccacgctcat ccccaccgcc ttgaactgca gcgcgaagta ggggatcgcg
     2881 gcgatcaccg ccagcaccgc caccgccgcc gccagcccgc gtgcgcgccc gtagcgcgac
     2941 gacaggaagt cggcgatcga gacgatccgg tggcgggtcg aggccagcac cagccgctcg
     3001 aggatgcgcc agccgaacag cagcagcagg atcggcccca ggtagatcgg caggaacccc
     3061 agtccggcgc gcgcggcggt tccgaccgcg ccgtagaagg tccacgacga gcagtacacc
     3121 gccagcgcca gggcatagac ggtggcgcgc gcgcgcggtc cctgcgccag ctgcggccgg
     3181 cgttcgccca gccaggcgac ggtgaacagc aggccgacgt aggcaagcgc caccaacagc
     3241 aggatccagc cggacatcat gcgtgcgcgc tccgcatccg cgatccggcc gagtgtacgg
     3301 ggcggcgggc gtggcccgcg cgcccggtat catccttgct gcatggacca cgcgcccgaa
     3361 accccaccgc tggccttgcc gcaatcccgc ccgtgggggc gggtgttcgt gcgacgggtc
     3421 gggctgggga ttgccggctt cgcgctgctg gtgctgctgg actggcagcc gtcgctgggc
     3481 ctgctgcgtg gcctgctgcc ggggatggtg ctgaagccgc tcgccgccct tgggttcctg
     3541 ctggcggggc tggtgctggc caacgaagcg cggcgggtgc cgtggccggc ggtttcggtg
     3601 ctgttgtcgg tcgcgatcgc ggtggccggc gcggccagcc tgcacgcgga ccttgccggc
     3661 gtggccctgg ccagcgatcg ctggttcccg gatccggaag cggccatcac cagcggcatc
     3721 gctggccgga tgtcgccgct caccgcatgc ggcctcttgt tgctgggcct gctgggcgcg
     3781 ctgcgcctcg atggtgcctg cctgcgcctg cgccacgggc tggcgctgct ggtgctgggc
     3841 atcgcgatgt tcaccctcgc cgcgtacggc cagcaggtgg gggacgccgc gttcctgccg
     3901 gtgccgctgc ttgccgccgg attgttcctg gccgcggcgc tggcatggct ggcggtgctg
     3961 cccgcgcgcg ggctgctgca cgtggtccat tccaccggca tcggcgggct ggtctcgcgc
     4021 cggctgttgt tgccggcgct gctgttgccc ggcctgctgg gggtgctggc gcggttggcc
     4081 gggtcgcgtg cggggtggtc ggccatcacc gtggcgtcga tccaggcgct tgccaccggg
     4141 gcgttgctgg cgtggatgat ctggtgggtg gcgaccctgc tggaacgcct gcagcggcag
     4201 cgcgacgagg tgcgggtgct gcatgacgaa agccacaccg atgcgctgac cggcctgccc
     4261 aaccggcgcg cgttcgacga ggcgatcgcc gtcctgggcc aagggcggcg cgggcacgac
     4321 cacgggggtt cgctgctgtt gctcgacctc gaccggttca aggactacaa cgaccgcttc
     4381 ggccaccagg cgggggacgc ggtgttgcgc caggtcggtg ccttgctgcg gcatgcggtg
     4441 cgcccgagcg acctgccggc gcgctacggc ggcgaggagt tcgcggtgtt gctgccggag
     4501 gccgggatcg aagaggcgat gcaggtggcg gaacgcatcg tccgtgcatt ccgggccgtg
     4561 gcatggcccg ggcgggcggt cacgatcagc gtgggcgccg cggcattggc cggttccgag
     4621 tccgcggacg cactggtcgg acgcgccgat gcggcgctgt acgcggccaa ggcggcgggc
     4681 cgcgaccgcg tggtgcaggc ggcggcgcct gcgcagcctg caccggcata gggatgccgg
     4741 aaacgcgaaa gcccgccgat gaggcgggct tcctgggtat ccggtgatac cgaaggctta
     4801 cttgatcttg ccttccttgt aggtcacgtg cttgcggacg acggggtcgt acttgctgac
     4861 ctccatcttc gccggcgtgt tcttcttgtt cttgtcggtg gtgtagaagt ggccggtgcc
     4921 ggccgaggag atcagacgga tcttgtcgcg cttgcttgcc atggtgtgat ctccttagac
     4981 cttctcgccc tgcttgcgca ggtccgcgag gatggcgtcg atgccattct tgtcgatggt
     5041 gcgcagggcc gcggcggaaa cgcggagctt gacccaacgg ttctcggaag cgacccagaa
     5101 gcggcgctcg tggaggttgg gcatgaagcg acggcgggtc ttgcgattgg aatgcgagac
     5161 gttgttaccg gaaaccgtgc gcttgccggt cacttggcag actttggaca ctgtgcacct
     5221 cgatggtgtt ttggttgtcg cccgtagccc gggagacggc ggccccgacg cagcccggag
     5281 ggcatgcggc acacgaaacg ggaaggcatg tcgcgctgcc aatggcgggc cggattcgtg
     5341 ttccggggcc gcccggaacc agccagaagg cggtgacgga cgcagcgagc cgcgcattat
     5401 gcggacaaat gcctttgcag gcaagcactt ggggttatgg gcggccgccc accagcgcgg
     5461 ggcgtccggc tagcggccct tggcctcggc
//
LOCUS       DAORUK010000140         5389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_18440_length_5389_cov_18.213348, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000140 DAORUK010000000
VERSION     DAORUK010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18440_length_5389_cov_18.213348"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PK818_08745"
     CDS             <1..224
                     /locus_tag="PK818_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HQY82854.1"
                     /translation="YNAANGEFGDMIEFGILDPTKVTRTALQNAASIAGLMITTEAMV
                     ADAPKKDEPAMGGAGGGMGGMGGMGGMDF"
     gene            446..1777
                     /locus_tag="PK818_08750"
     CDS             446..1777
                     /locus_tag="PK818_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82855.1"
                     /translation="MSQLQWDVRVARSPEPEAQAIVRATHALDAGDRARADQGYRDLQ
                     RQGMAHPASWSNLAALGIALGDAGGARANAQRALQLDRDSVDAWVNLGVASWQLGQRR
                     DAAMAMHRALALDPGLEAAALNYARMLQLVDRPGEARKTLELAVLANPDAWRLRQALA
                     QQARLDGDAAAARQQALEALARLGPHLQPQVDAAPRPGAENEAAAAAASANVQRALFA
                     AGDALDAAGVPFHLIGGTLLAIHRDGRPFPHDKDVDLALPYDSDRDAVAAAFAGEFRP
                     VLRAEDPRSHAAREWVMGFTHQQSGIGVDLMFAREQDGMMRFELGWPDYLACEMPAYP
                     LQPLHWGGRDWLAPAPPRQYLDAIYGPDWAGPAVIRGFDRRWFDTQVSNPSRTPESLP
                     RAVTLVLLRLLQALQAGQWEKARALCIQVQAREPLPEVGAMLARLERVQAT"
     gene            1777..2442
                     /locus_tag="PK818_08755"
     CDS             1777..2442
                     /locus_tag="PK818_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HQY82856.1"
                     /translation="MTPAAGDVSFLVPAAGIGQRLGMGPKAFLELRGRPLLCWIADKA
                     LQVAGEVLVAVPADRVDAAAELLPGCRVIAGGGSRHDSVARLARAATGEWCLVHDAAR
                     PFASVALFRGVLAAARGSGCAGAFLDPEVPVARIGGGLVLEGFDRDQVGVFQAPQAFA
                     RALLLDLFAQAEAAGRRPQSTLQLALQAGVPVAAVPGEKHNIKLTTPEDWRHAALLED
                     HLK"
     gene            2439..3674
                     /locus_tag="PK818_08760"
     CDS             2439..3674
                     /locus_tag="PK818_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HQY82857.1"
                     /translation="MSSGNLPRTQHPELFRWSPALDPADYVQARYSVTSVHDGEATAL
                     GMAMEQSAGTVMIRGYVSPGQVERWTIRVLAIEAQAEPPASGLQPYQLATDVYAEQQQ
                     QAQTWSVTLAIPRVLLAGKAGQLFNVVVGELPRLGFLTRFRLEALALPEDFGPGPGFG
                     VAGIRARLQCPRGPLLCRSMRPAVGLDADTMARLNRDVLEAGYHLVKDDELQVFADNH
                     AFRQHVEAMVAARDQARATTGERKGYLANLICEADELAERWDIIKTLGVDGALVAPAL
                     QGFSTLSMLARERRMPLLAHNTFSDLLTRHPGWGIEHAVLCGLYQAFGADWIVTSGEY
                     GATDGVGMEDAWPFGRPQRAMPILQGGKHPQGLPDYRAAIGSDDYMLIVASWVDGHAD
                     GLPAAARAFRTAIDAQPPA"
     gene            3973..5235
                     /locus_tag="PK818_08765"
     CDS             3973..5235
                     /locus_tag="PK818_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82858.1"
                     /translation="MAAEGIQQLQAGDRERAQRSYAEASTQANSWQCWTNLAALALGL
                     EDFEAALANARKAIVIDPGHADAWVNAGVASWRKGARKDGARLMAHALQLAPAHATAA
                     INYSYMLRTVERLREAHEVLAGARSRGARTPGVLRAMAELERILERSGECRANALEAL
                     RILAGNPWPPPVTGTLSARVAFEAATWRGVLVDCLRRLAPLHCDPCMAGGTVRMFAFE
                     GGLPAARKDIDIVIDARVSHELLRQAFAQGYRPFSRQGQLVPGSGGIGVLGLVHEASN
                     IPIDLMLQDRANGRWRGSFGAPDHLVFDTPAFSIESVACAGIEVPLPVPQPMHEYLRW
                     FYGDDWTQPTQAVAGRAVPREYVQKGMHCPALDPGSRPAALNRALLSLLTALHEGWVE
                     MAAALCWQILAMEPLDEVQRVQARLSQA"
BASE COUNT          794 a         1740 c         2010 g          845 t
ORIGIN      
        1 gctacaacgc cgccaacggc gagttcggcg acatgatcga gttcggcatc ctggacccga
       61 ccaaggtcac ccgcaccgcg ctgcagaacg cggcgtcgat cgccggcctg atgatcacca
      121 ccgaagcgat ggtggcggat gcgccgaaga aggacgagcc cgccatgggc ggcgccggcg
      181 gcggcatggg cggcatgggt ggcatgggcg gcatggattt ctgatccgcg ccaccgcagc
      241 aacggaaagg ccccgcttcg gcggggcttt ttcgttgcga cgtatcattg gcactcgaca
      301 catcgacgga aaacggatgt ccacattgca tttcgatgtg cgcgtggtgc cgccggccga
      361 accggcggcg cagcggttgc tgctgccggc acggcaaccc gcgccgggcg ccgggtgaac
      421 gcaggtcgat ccagcagggg gattcgtgag ccagttgcag tgggacgtcc gggttgcgcg
      481 atccccggaa ccggaggcgc aggcgatcgt gcgggcaacg cacgcgctgg atgccggcga
      541 tcgcgcgcgt gccgaccagg gctaccgcga tctgcagcgg cagggcatgg cgcatcccgc
      601 gagctggtcc aacctggccg cgctcggcat cgccctcggg gatgccggcg gcgcgcgtgc
      661 gaacgcgcag cgggccttgc agctggatcg cgacagcgtc gatgcgtggg tcaacctcgg
      721 ggttgccagc tggcagctgg gccagcggcg cgatgctgcc atggccatgc accgcgcgct
      781 ggcgctggat ccgggcctgg aggccgcggc cttgaactat gcccggatgc tgcaactggt
      841 cgatcgcccg ggcgaggcgc gcaagacact ggaactggcg gtgctggcga acccggacgc
      901 atggcggctg cgccaggcgc tggcgcaaca ggcgcgcctg gatggggacg ccgcggccgc
      961 gcggcagcag gcactcgagg ccctggcgcg cctgggaccg cacctgcagc cgcaggtcga
     1021 cgccgcaccc cggcctggtg cggagaacga agccgcggcg gcagcggcaa gcgccaacgt
     1081 gcagcgggcg ctgttcgccg ccggcgatgc gctggatgcg gccggcgtgc ccttccacct
     1141 gatcggcggg accctgctgg cgatccatcg ggatggccgt ccatttccgc atgacaagga
     1201 cgttgacctc gcgctgccgt acgacagcga ccgcgatgcc gtcgcggcgg cgttcgccgg
     1261 ggaattcagg ccggtcctgc gcgcggagga tccacgcagc cacgcggcgc gcgaatgggt
     1321 catgggattc acccatcagc agagcggaat cggcgtcgac ctgatgtttg cccgcgagca
     1381 agacgggatg atgcgctttg aacttggctg gccggattac ctggcctgcg aaatgccggc
     1441 ctatccgctg cagcccttgc attggggcgg acgcgactgg ctggcgcctg cgccaccacg
     1501 gcagtacctg gacgccatct acggcccgga ctgggcgggt ccggccgtga tccgcggttt
     1561 cgatcgccgc tggttcgata cccaggtatc gaacccgtcg cgcacgccgg agtcgctgcc
     1621 gcgtgcggtg accctggtgc tgttgcgcct gctgcaggca ttgcaggccg ggcagtggga
     1681 gaaggcgcgc gcgctttgca tccaggtgca ggcgcgtgag ccgcttcccg aggtcggcgc
     1741 gatgctggcg cggcttgaac gcgtgcaggc gacctgatga cgccggccgc cggcgatgtg
     1801 tcgtttctgg tccccgccgc cggcatcggc cagcgcctgg gcatgggtcc gaaggccttc
     1861 ctcgaactgc gcgggcgccc gttgctgtgc tggatcgccg acaaggcgct gcaggtggcc
     1921 ggcgaggtgc tggtcgcggt gcctgcggat cgcgtcgatg ccgcggcgga attgctgccg
     1981 gggtgccggg tgatcgccgg cggcggaagc cggcatgaca gcgtggcgcg gctggcgcgg
     2041 gcggcgaccg gcgaatggtg cctggtgcac gacgcggcgc gcccgttcgc cagcgtcgcg
     2101 ctgttccgcg gcgtgctggc cgcggcgcgc ggatccgggt gcgccggcgc gttcctggat
     2161 cccgaggtgc cggtggcgcg cattggcggc gggctggtgc tggagggctt cgaccgcgac
     2221 caggtcgggg tgttccaggc gccgcaggcg ttcgcgcgcg cgctgctgct ggacctgttc
     2281 gcgcaggcgg aggccgcggg ccggcgcccg caatcgaccc tgcaactggc gctgcaggcg
     2341 ggtgtgccag tggcagccgt gcctggcgaa aaacacaaca tcaagctgac gacgccagaa
     2401 gactggcggc atgcggcact actggaggac cacctgaaat gagttccgga aacctgccac
     2461 gcacgcaaca cccggagctg ttccggtggt ctcccgcgct ggatcctgcg gactacgtgc
     2521 aggcgcgcta cagcgtgacg tcggtgcacg atggcgaggc cactgcgctc gggatggcga
     2581 tggaacagag cgcgggaacc gtgatgatcc gcggttacgt ttcgcccggg caggtggaac
     2641 gctggaccat ccgggtgctg gcgatcgagg cgcaggccga acctccggca tccgggctgc
     2701 agccgtacca gctggccacc gatgtctacg cggaacagca gcagcaggcg caaacctggt
     2761 cggtgaccct ggcgattccc cgcgtcctgc tggcaggcaa ggcgggacag ctgttcaacg
     2821 tcgtcgtcgg cgaattgccg cggctgggct tcctcacccg cttccggctg gaggcgctgg
     2881 ccttgccgga ggatttcggg cccgggccgg gcttcggggt ggcgggcatc cgcgcgcgcc
     2941 tgcagtgccc gcggggtccc ttgctgtgcc gttcgatgcg cccggcggtg ggcctggatg
     3001 ccgacaccat ggcgcggctc aatcgcgatg tgctggaagc cggctatcac ctggtcaagg
     3061 acgacgagtt gcaggtgttc gcggacaacc acgcatttcg ccagcatgtc gaggcgatgg
     3121 tcgccgcccg tgaccaggcg cgcgccacca ctggcgagcg caagggctac ctcgcgaacc
     3181 tgatctgcga ggccgatgag ctggccgaac gctgggacat catcaagacg ctgggggtgg
     3241 atggcgcgct ggtggcgccg gcgctgcagg gattcagcac cctgtcgatg ctggcgcgcg
     3301 aacggcgcat gccgctgctg gcgcacaaca ccttcagcga cctgctgacc cgccacccgg
     3361 gctggggcat cgaacatgca gtgctgtgcg gcctctacca ggcgttcggt gccgactgga
     3421 tcgtgacctc gggcgagtac ggggccaccg acggcgtcgg catggaggac gcatggccat
     3481 tcgggcgtcc gcagcgggcg atgccgatcc tgcagggcgg caagcatccg caagggctgc
     3541 cggattaccg cgcggcgatc ggcagcgacg actacatgct gatcgtggcg tcgtgggtgg
     3601 atggccatgc cgatggcctg cccgccgccg cacgcgcgtt ccgcaccgcg atcgacgccc
     3661 agccgccggc ctgaggcgtg ggcaacagga ccggtggcac ccggggcttg gcgcgctacc
     3721 gtggggcaat cgggagcaac gattccattc gggtcgtcgc gtcgtcggtt gatggccatg
     3781 cgcgcggttt gaatggccct gtctgcgcgc cctgcaacac catctgcacc tggtcatgcg
     3841 catgagcggg atgattcttg caggcgacgc atctcgctat gctgatcgtc ccttcgcaga
     3901 ctggaaggcg ccatgttgtc cgcatgcgtg atcagttccg ccgcgccgtc caggttgcct
     3961 ctcggcgcgc acatggccgc cgaaggcatt cagcagttgc aggcgggcga tcgcgagcgc
     4021 gcgcagcgtt cgtatgcaga ggcgtccacc caagcgaaca gctggcagtg ctggacgaac
     4081 ctggcggcgc tcgcgctggg attggaggac ttcgaagccg cccttgccaa tgcacgcaag
     4141 gcaatcgtca tcgatccggg acatgcggat gcctgggtga atgccggcgt ggccagttgg
     4201 cggaaaggtg cgcgcaagga tggggcccgc ctgatggcgc atgcactgca actggcgccg
     4261 gcgcacgcaa ccgcggcgat caactattcc tacatgttgc ggacggtgga gcggctccgg
     4321 gaggcgcatg aggtgcttgc aggggcacgc agccggggtg cgcgcacgcc aggggtgctg
     4381 cgggcgatgg cggaactgga acggatcctg gaacggtccg gcgagtgccg cgcgaacgcg
     4441 ctggaagcgt tgcggatcct ggccgggaat ccctggccgc cgcccgtcac gggcacattg
     4501 tccgcgcgcg ttgcattcga agcagcgaca tggcgtggcg tactggtcga ttgcctgcgg
     4561 cggctggcgc cattgcactg cgacccctgc atggcgggcg gcacggtgcg catgttcgcg
     4621 ttcgaaggcg ggctgccggc ggcgcgcaag gacatcgaca tcgtcatcga cgcgcgggta
     4681 tcccacgaac tgttgaggca ggcctttgca cagggctacc gcccgttttc gcgccagggg
     4741 caactggtcc ccggcagtgg cgggatcggc gtgctgggac tggtccacga ggcaagcaat
     4801 atcccgatcg acctgatgct gcaggaccgg gccaacgggc gctggcgggg atcgttcggc
     4861 gcgccggatc acctggtgtt cgataccccg gcgttctcca tcgagagcgt ggcctgcgcc
     4921 ggcatcgagg ttccgctgcc ggtgccgcag cccatgcacg agtatctccg ctggttttac
     4981 ggcgacgact ggacgcagcc cacgcaggcg gttgcgggac gtgcggtgcc cagggagtac
     5041 gtgcagaagg gaatgcactg cccggccctg gacccgggta gccgcccggc ggcgctcaac
     5101 cgggcgttgt tgtcgttgtt gaccgcattg catgagggat gggtcgagat ggcggctgcg
     5161 ctgtgctggc agatcctggc gatggagccg ctggatgaag tccagcgcgt gcaagcgcga
     5221 ctgtcgcagg cttgatccga tcgcaggccc ggcggttcgc gggcgcgtga attcagcggc
     5281 aatgcgatat ccggcctgca tcgccgcttc cgttttgccg gccgggcgcg gatcatcggc
     5341 tgtttcccga atcggtgccg cgcgtggcga cttcccggaa cgacgatgt
//
LOCUS       DAORUK010000141         5375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_18521_length_5375_cov_18.903195, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000141 DAORUK010000000
VERSION     DAORUK010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18521_length_5375_cov_18.903195"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="PK818_08770"
     CDS             complement(<1..142)
                     /locus_tag="PK818_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EtfB protein"
                     /protein_id="HQY82859.1"
                     /translation="MKILVAYKRVVDYNVRIQVKPDGSGVVTDGVKLSANPFDDIALE
                     EAL"
     gene            303..1019
                     /locus_tag="PK818_08775"
     CDS             303..1019
                     /locus_tag="PK818_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014506572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HQY82860.1"
                     /translation="MAAKSKQYPDAATALAGLVADGQTLAVGGFGLCGIPEALIAALR
                     DSGVTGLTAISNNAGVDGFGLGQLLETRQIRKMISSYVGENKEFERQFLGGELELEFN
                     PQGTLAERLRAGGAGIPAFFTATGYGTVVAEGKETRQIDGRWYVLETALKADVSLVKA
                     WKADTAGNLVFRKTARNFNPAVAMAGKVCVAEVEELVEIGAIDPDHVHLPGIYVDRIV
                     VNAIPEKRIEQRTIRQPEEN"
     gene            1021..1647
                     /locus_tag="PK818_08780"
     CDS             1021..1647
                     /locus_tag="PK818_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HQY82861.1"
                     /translation="MAWTRDQMAERAARELSDGAYVNLGIGLPTLVANHIPDGMHVWL
                     QSENGLLGIGPYPLDEEVDADLINAGKQTVTAVDGASYFGSQESFAMIRGGHIDLAIL
                     GAMEVTDKGDLANWMVPGKLVKGMGGAMDLVAGVKRVVVLMEHTTKTGAPKIIPQCTL
                     PLTGLGVISRIITELGVFDVTAEGLKLVELADGVSREEAVQKTGVPLH"
     gene            complement(1788..3413)
                     /locus_tag="PK818_08785"
     CDS             complement(1788..3413)
                     /locus_tag="PK818_08785"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HQY82862.1"
                     /translation="MSQPEPIERDVMEYDVVAVGAGPAGLAFAIRLKQLDPALSVCVI
                     EKASTIGAQILSGAVIETGPLDALLPDWRKSPPPICVDATDDEFWLLSKTGGRKLPVP
                     PGMHNRGNVIVSLGAMCAWLAPQAEALGVEIYPGFAAAETLHDDTGKVIGVRIGDMGV
                     ARDGSHKPGYTAGIDIRAKVTVLAEGARGHLTKRLVKRFKLDADSDPQGYSIGIKELW
                     QVPEERVSPGRIVHSFGWPADSRTYGGSFLYHLDKGRIALGYVSGLDYQDPNYQPWEA
                     FQQWKNHPMVKPLLEGGSIVSSGARAIVTGGYQSLPKVEMPGALLIGDTAGLLNVPKV
                     KGTHQAIRSGMLAAEHLVATRLAAEGFDARLRGSEAMAELKKVRNVKPGFKRGLWFGM
                     LNAAWETVTGGLSPWTLKNKPDWSALHKLGEHQQPKRDYLERTLAPRDRLASVYFAAT
                     EHDEDQPVHLKVADTDLCASRCVVEYDNPCTRFCPAGVYEMVADDNAPHGKRLQVNSA
                     NCVHCKTCDIKDPYEIITWVTPEGGSGPNYQNL"
     gene            complement(3490..4119)
                     /locus_tag="PK818_08790"
     CDS             complement(3490..4119)
                     /locus_tag="PK818_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011052365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HQY82863.1"
                     /translation="MLRIAAALLAAATLAGCALGGDKVAPTVFAPQPAYQADPAWPTV
                     RWQLGISRPEASRMLDSTRIAVRPVPGELQVYKGASWARTPVEMLEDGVLRTLEDSGR
                     IPAVARQGSGMSADYRLLMDLRHFEADYAAGATPAAVLEVSAKLLHAQDKSVVASRVF
                     RQAQPAAGTEVAQVADAFAQALAATSQELAGWVLASGQAHAAAHPDGNR"
     gene            complement(4128..5054)
                     /locus_tag="PK818_08795"
     CDS             complement(4128..5054)
                     /locus_tag="PK818_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HQY82864.1"
                     /translation="METRANYVLIGAFTLLTAVFLLLFTLWATKYSADRNWRQFQVIF
                     SEPVTGLTEGGGVQYNGIAVGTVDKLRLDPDDARKVIALLKVQADTPVKVDTKAKLSQ
                     QGITGVPFIQLTGGSPQAALLEPDDRGDVPVIHTEPSALQNIADTANRLVARMDQVLS
                     EQNIERITATIGHIEQATGSIAGQREDIAALIVNTREATDSLKATLATANGSVERIDR
                     EVVAQLPALMAKLDATVGKLDSVAGNANAMIAENRPALQSFTNDGLAQLGPTLAELRV
                     LVRDLRRITDRMEGSPARYLLGRDAPKEFEPE"
     gene            complement(5091..>5375)
                     /locus_tag="PK818_08800"
     CDS             complement(5091..>5375)
                     /locus_tag="PK818_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007969506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY82865.1"
                     /translation="PPLLFLDEPTAGLDPIGAAAFDELIRNLQQALGLTVFLITHDLD
                     TLYAICDRVAVLADRKLLAAAPVAELETLDHPWVQEYFNGPRGRAAREAT"
BASE COUNT          786 a         1828 c         1876 g          885 t
ORIGIN      
        1 gcagggcttc ttccagcgcg atgtcgtcga acgggttggc cgacagcttg acgccatcgg
       61 tgaccacgcc ggaaccgtcc ggcttgacct ggatgcggac gttgtagtcc accacgcgct
      121 tgtaggcgac gaggatcttc atcgggcggg gaatcctggg aaagacgtgg ccgccatttt
      181 acccgagggt gactgggtat ttgctcgaag cgcggggccc cgcgccgggt gcccgaaggt
      241 acggtgggcg agccgcggct tgccggtaaa ataccggttc cccgccccga tggagccgcc
      301 ccttggccgc caagagcaag caatacccgg atgccgcgac tgcccttgcc gggctggtgg
      361 cggacggcca gaccctcgcc gtcggcggct tcggcctgtg cggcatcccc gaggccctga
      421 tcgccgcgct gcgcgattcc ggggtcaccg gcctgaccgc gatctccaac aacgccggcg
      481 tcgacggctt cggcctcggc cagctgctgg aaacccgcca gatccgcaag atgatttcgt
      541 cctacgtcgg cgagaacaag gagttcgagc gccagttcct cggcggcgaa ctggagctgg
      601 agttcaaccc gcagggcacc ctcgccgagc gcctgcgcgc cggcggtgcc ggcatcccgg
      661 cgttcttcac cgccaccggc tacggcacgg tggtggccga gggcaaggaa acccggcaga
      721 tcgacggccg ctggtacgtg ctggaaaccg cgctgaaggc ggacgtctcg ctggtcaagg
      781 catggaaggc ggacaccgcc ggcaacctgg tgttccgcaa gaccgcgcgc aacttcaacc
      841 cggcggtggc gatggccggc aaggtctgcg tggccgaggt cgaggaactg gtggagatcg
      901 gcgcgatcga tcccgaccac gtgcacctgc ccggcatcta cgtggaccgg atcgtggtca
      961 acgccatccc ggagaagcgc atcgaacaac gcaccatccg ccagccggag gagaactgac
     1021 atggcctgga cccgcgacca gatggccgag cgcgccgcca gggaactgag cgatggcgcc
     1081 tacgtgaacc tcggcatcgg cctgccgacg ctggtggcca accacatccc ggacggcatg
     1141 cacgtgtggc tgcagagcga gaacggcctg ctcgggatcg gcccgtaccc gctggacgaa
     1201 gaggtcgatg ccgacctgat caacgccggc aagcagaccg tcaccgcggt cgatggcgcc
     1261 agctacttcg gcagccagga gtcgttcgcg atgatccgcg gcggccacat cgaccttgcg
     1321 atcctcggtg cgatggaggt caccgacaag ggcgacctcg ccaactggat ggtgccgggc
     1381 aagctggtca agggcatggg cggcgcgatg gacctggtgg ccggggtcaa gcgggtggtg
     1441 gtgctgatgg aacacaccac caagaccggc gcgccgaaga tcatcccgca atgcacgctg
     1501 ccgctgaccg gacttggcgt gatcagccgc atcatcaccg agctgggcgt gttcgacgtg
     1561 accgcagaag gcctgaagct ggtcgaactg gcggacggcg tgagccgcga ggaagcggtg
     1621 cagaagaccg gcgttccgct gcactgaggc atcgcggggg ccgctgagcg gcgagcccct
     1681 tccgcgaatg tcccccggca gcgacctgtc ggccgctgcc tcctccttga ctccgctaca
     1741 ggggcccgcc gctcagcggc ccccgcgcgt gttgcgcgcg cgcaaggtca caggttctgg
     1801 taattcggcc cgctgccgcc ttccggcgtg acccaggtga tgatctcgta cgggtccttg
     1861 atgtcgcagg tcttgcagtg cacgcagttg gcggaattga cctgcaggcg cttgccgtgc
     1921 ggggcgttgt cgtcagccac catttcgtag acgccggcgg ggcagaagcg ggtgcaggga
     1981 ttgtcgtatt ccaccacgca gcggcttgcg cacaggtcgg tgtcggcaac cttgaggtgg
     2041 accggctggt cctcgtcgtg ctcggtggcg gcgaagtaca cgcttgccag gcggtcgcgc
     2101 ggcgcgagcg tgcgttcgag gtagtcacgc ttcggctgtt ggtgttcgcc gagcttgtgc
     2161 agggcgctcc agtccggctt gttcttcagc gtccacggcg acagcccgcc ggtgacggtt
     2221 tcccacgccg cgttcaacat gccgaaccac agcccgcgct tgaagcccgg cttgacgttg
     2281 cggaccttct tcagttccgc catcgcctcc gagccgcgca gcctggcgtc gaagccttcg
     2341 gccgcgagcc gggtggcgac caggtgctcg gcggcgagca tgccgctgcg gatcgcctgg
     2401 tgggtcccct tcaccttcgg cacgttgagc aggccggcgg tgtcgccgat cagcagcgca
     2461 cccggcatct ccaccttcgg cagcgactgg tagccgccgg tcacgatcgc ccgcgcgccg
     2521 gaggacacga tgctgccgcc ttccagcagc ggcttcacca tcgggtggtt cttccattgc
     2581 tggaaggctt cccacggctg gtagttcggg tcctggtaat ccagcccgct gacgtagccc
     2641 agcgcgatcc ggcccttgtc caggtggtac aggaagctgc cgccgtaggt cctgctgtcc
     2701 gccggccagc cgaagctgtg cacgatcctg ccgggtgaca cgcgctcttc cggaacctgc
     2761 cacagctcct tgatgccgat cgaataaccc tgcgggtcgc tatccgcgtc gagcttgaag
     2821 cgcttgacca gccgcttggt caggtgcccg cgcgcgcctt cggcaagcac cgtcaccttg
     2881 gcgcggatgt cgatcccggc ggtgtagccg ggcttgtgcg atccgtccct ggccacgccc
     2941 atgtcgccga tgcgcacgcc gatgaccttg ccggtgtcgt cgtgcagggt ttcggcggcg
     3001 gcgaagccgg gatagatctc gacccccagc gcttccgcct gcggcgccag ccaggcgcac
     3061 atcgcgccca ggctgacgat cacgttgccg cggttgtgca tcccgggcgg caccggcaac
     3121 ttgcggccgc cggtcttgct gagcagccag aactcgtcgt cggtggcgtc cacgcagatc
     3181 ggcggcgggc tcttgcgcca gtccggcagc agcgcatcca gcgggccggt ctcgatcacc
     3241 gcgccggaca ggatctgcgc cccgatcgtg ctggccttct cgatcacgca caccgaaagc
     3301 gcggggtcca gttgcttgag ccggatcgcg aacgcaaggc cggccggccc cgcgccgacg
     3361 gcgacgacgt cgtactccat gacgtcccgt tcgatcggct caggctggct catgctcgcg
     3421 ctccgcggtg gatgccgcga ttttccccgg tttgcaccgg atgcggcaaa tccccgcggc
     3481 gcgacgcggt cagcggttgc cgtccgggtg cgcggcggcg tgcgcctggc cgctggccag
     3541 cacccagccg gcaagctcct ggctggtggc cgccagcgcc tgcgcgaagg catcggcgac
     3601 ctgcgccacc tcggtgccgg ccgcgggctg cgcctgccgg aacacacggc tggcgaccac
     3661 cgacttgtcc tgtgcgtgca gcagcttggc gctcacctcc agcaccgcgg cgggggtggc
     3721 gccggcggcg tagtcggcct cgaagtggcg caggtccatc agcagccggt aatcggccga
     3781 catcccgctg ccctggcggg ccaccgcggg gatccgcccg gaatcctcca gcgtgcgcag
     3841 cacgccgtcc tccagcatct ccaccggcgt gcgcgcccat gacgcgccct tgtagacctg
     3901 cagttcgccc ggtaccggcc gcaccgcgat gcgggtgctg tccagcatgc gcgaggcttc
     3961 cgggcgggag atcccgagtt gccagcgaac cgtcggccag gctggatcgg cctggtaggc
     4021 gggctgcggc gcgaacacgg tcggtgcgac cttgtcgccg cccagcgcgc agccggcgag
     4081 cgtggcggcg gccagcaggg cggccgcgat gcgaagcatt ggcgtgttca ttcgggttcg
     4141 aactccttgg gcgcgtcgcg gcccagcagg tagcgtgccg ggctgccttc catgcggtcg
     4201 gtgatccggc gcaggtcgcg gaccagcacc cgcaactcgg ccagcgtcgg gccaagctgg
     4261 gcgaggccgt cgttggtgaa gctctgcagc gcgggtcggt tctccgcgat catcgcgttg
     4321 gcgttgccgg cgaccgaatc cagcttgccg acggtggcat cgagcttggc catcagcgcc
     4381 ggaagctgcg ccaccacctc gcggtcgatg cgctcgaccg agccgttggc ggtggcgagg
     4441 gtggccttca gcgaatcggt ggcctcgcgg gtgttgacga tcagggcggc gatgtcctcg
     4501 cgctggccgg cgatgctgcc ggtggcctgc tcgatgtgcc cgatggtggc ggtgatgcgc
     4561 tcgatgttct gctcgctcag cacctggtcc atccgcgcca ccaaccggtt ggcggtgtcg
     4621 gcgatgttct gcagggcgga aggttcggtg tggatcaccg ggacgtcgcc gcgatcatcc
     4681 ggctccagca gcgccgcctg cgggctgccg ccggtgagct ggatgaacgg caccccggtg
     4741 atgccctgtt gcgacagctt ggccttggta tccaccttga ccggggtgtc cgcctgcacc
     4801 ttgagcagcg cgatcacctt gcgcgcgtcg tcgggatcca ggcgcagctt gtcgacggta
     4861 ccgactgcga tgccgttgta ctgcacgccg ccaccctcgg tcaggccggt caccggctcg
     4921 gagaagatca cctggaactg gcgccagttg cgatcggcgc tgtacttggt cgcccacagc
     4981 gtgaacagca gcaggaacac cgcggtcagc agggtgaagg cgccgatcag tacgtagttg
     5041 gcacgggttt ccatctcgca gtccttcttg cttgcgccga caccggtggc tcaggtcgct
     5101 tcacgcgcgg ctcgcccacg cggcccgttg aaatattcct gcacccaggg gtggtccagc
     5161 gtttccagtt cggcgacggg ggccgccgcc agcaacttgc ggtcggccag caccgccacc
     5221 cggtcgcaga tagcgtatag cgtgtcgagg tcgtgggtga tcaggaacac ggtcaggccc
     5281 agcgcctgct gcaggttgcg gatcagttcg tcgaacgcgg ccgcgccgat cgggtcgagg
     5341 ccggcggtcg gttcgtcgag gaacagcagc ggcgg
//
LOCUS       DAORUK010000142         5309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_18879_length_5309_cov_16.921013, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000142 DAORUK010000000
VERSION     DAORUK010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18879_length_5309_cov_16.921013"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1207
                     /locus_tag="PK818_08805"
     CDS             110..1207
                     /locus_tag="PK818_08805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQY82866.1"
                     /translation="MTAVRVLHFVTGGFSGGATQVAIQLVNAAREGDAVEPLLVLRRK
                     RRTPPERIAELARAGVPLRMVPGWSHLASVIALVRICREFRPDVLVAHGFSEHLWGRY
                     AGVLAKVPHLVHVEHNTRERYTAWRLAQARWLAKRTDRIVGCSEGVRRVLLEMGMPAA
                     RTIAIPNGIRLEPFAEADAHPFAQRVPGIVMVARYSKQKDHATLLRAVALLRERGLAP
                     PVLFAGGGKALHRKPLEKLANQLGIGEQVQFPGVVRNVPELLLGHQLAVLATHYEGMP
                     LALLEGMAAGCAVIGSAVPGVREVIEDGIDGRLVAESDPVAMADAIEQLLRDPAQASR
                     LAVNARKVALERHGRELMNQRYQALFLELAR"
     gene            complement(1204..2235)
                     /gene="galE"
                     /locus_tag="PK818_08810"
     CDS             complement(1204..2235)
                     /gene="galE"
                     /locus_tag="PK818_08810"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HQY82867.1"
                     /translation="MRDNRRTTSLLAYMPDSILVVGGAGYIGSHMVKRLRRAGLHAVV
                     ADDLSSGHREAVLGAPLHVGDIGDAAFVDALLGEVRPAAVMHFASCIQVGESVAEPAK
                     YYRNNLTATQVLLDGMRAHGVNRFIFSSTAAIFGDPQYVPIDEAHPKAPINPYGRSKW
                     FVEQMLEDFDRAYGLKAVCLRYFNAAGADPEGELGECHQPETHLVPLILQVAAGRRPH
                     ITVYGEDYATADGTCVRDYIHVEDLADAHLLALRQLLAGGGSARYNLGNGNGYSVREV
                     IAAVRRVTGHPIPVVVGQRREGDPPVLVADARAARESLGWTPRFADLDTLVAHAWAWE
                     RRHARWARS"
     assembly_gap    2416..2515
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2516..4086
                     /locus_tag="PK818_08815"
     CDS             <2516..4086
                     /locus_tag="PK818_08815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQY82868.1"
                     /translation="WAAVALVSIFLHGPLPLYSTRSLAVAWEMWDAGSWLVPLFNGEP
                     YSHKTPLLPWLIHLGWLLGGVSDLWPRVLVVLLGTLAIAQTGLLAGRLYPRRPALPAL
                     AAWAMAGFWYFFLFSLQVMYELPLMVAVLGGLLALCRRDGAGWNPWWPGVALAVGFGL
                     LAKGPVVLLHLGVPLLAARWWHPAAQRDGARFAKRALLAVLAGIGVFALWLLPALTLG
                     DPEYRQALLVTQTAGRIRDSFDHAEPWWWYLPLLLVLMAPWWWSRWWWAQVPVLWRDA
                     GNRFAWIWVIGVVLAFSAISGKNPYYLLPEFAGFAILLALAADTRPRLGIAAGVGVLL
                     LAALLLGARVLAGHVERPFPEALLDSLPLAGGVLAVVGIGLLRWRSLPVQAGGVLLAT
                     ALLHAALTPLIKAQYDMTATAQALGQAQRAGTRIAFLGEYQLQFHFAGRLRAPIEVVD
                     EAGARAWATQHPRDLIVVNAREDWRAPGPHPVIQQRFRSRWVQVWRASDWRALPAQQL
                     PLQAAARQLRSQPR"
     gene            complement(4039..>5309)
                     /locus_tag="PK818_08820"
     CDS             complement(4039..>5309)
                     /locus_tag="PK818_08820"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HQY82869.1"
                     /translation="IRIRSGWLHRSLREIPFARIHNVNLHQSLLHRMFGVAEVRLESA
                     GGTKPEAQMRVLRLDQALALEALVRRRGNPGVAAGEADAAPPLLALSTGELVRLGLIS
                     NRGMLVVAAAFGALAQTGEDVFGVLVERWGRLLFGRVSEFMGEHAHDSTLGIGLAAVS
                     LVVVALVLVRLLSVLLAILQFHGFTLREDDGRLSVERGLLARSRSSARRRRIQAWSLR
                     EGVLHRWFRRRSLRVDTAAGQRGAQEQHTLKDIAPIASPGHCDALIRHFLPDAGWDAL
                     DWQPLHRGAWLRIAMPGLLLVLVAVVAGCWRFGGIGLLGLLLLPLQLWRARRIAAACG
                     YADNGRLVAWRTGWLAKTWNFAEVDKVQALRLAQSPLDRRLGMASLLLDTAGASPFGA
                     PLHLHHLPLAAAQALSARLGAQLARRRLQW"
BASE COUNT          695 a         1788 c         1923 g          803 t
ORIGIN      
        1 agggcagcgt gctgccggcg tgcgtggaca ccgcgcgcgc gcgcgacatg ggcaatgcca
       61 accggcgccg ctggtggctg ctgcctggcg cggcgacccg ggagtgccga tgacggcggt
      121 gcgcgtactg cacttcgtca ccggcggctt ctcgggtggc gcgacccagg tggcgatcca
      181 actggtgaac gcagcccgcg agggcgatgc ggtggaaccg ctgctggtgc tgcggcgcaa
      241 gcggcgcacg ccgccggagc gcatcgccga gctcgcgcgt gcaggggtgc cgctgcgcat
      301 ggtgccgggc tggtcgcacc tggcgtcggt catcgcgctg gtccgcatct gccgtgaatt
      361 ccgtccggac gtgctggtcg cgcacggctt cagcgaacac ctgtgggggc gctatgccgg
      421 cgtgctggcc aaggtgccgc acctggtgca cgtcgagcac aacacccgcg agcgctacac
      481 cgcgtggcgg ctggcacagg cgcgctggtt ggcgaagcgc accgaccgca tcgtcggttg
      541 ttccgaaggc gtgcgccggg tgctgctgga gatgggcatg ccggcggcgc gcaccatcgc
      601 gatcccgaac ggcatccgcc tcgaaccctt cgccgaagcc gacgcgcatc cgttcgcgca
      661 gcgggttccc ggcatcgtga tggtcgcccg ctattcgaag cagaaggacc acgcaacgct
      721 gttgcgcgcg gtggcgctgt tgcgcgaacg cgggctggca ccgcccgtgc tgttcgccgg
      781 tggcggcaag gcgctgcacc gcaagccact ggagaagctg gcgaaccagc tcggcatcgg
      841 cgaacaggtg cagtttcccg gcgtggtgcg aaacgtcccg gagttgttgc ttggccacca
      901 gctggcggtg cttgccaccc attacgaggg catgccgctg gccctgctcg aaggcatggc
      961 ggccggatgc gcggtgatcg gcagtgcggt gccgggcgtg cgcgaggtga tcgaagacgg
     1021 gatcgatggc cgcctcgtcg ccgagtcgga cccggtggcc atggccgatg ccatcgaaca
     1081 actccttcgc gatccggcgc aggcatcacg gctcgcggtg aacgcacgca aggtggcgct
     1141 ggaacggcat ggccgcgaat tgatgaacca gcgttaccag gcgctgttcc tggaactggc
     1201 gcgctagctg cgcgcccacc gtgcatgccg gcgttcccac gcccaggcat gcgccacgag
     1261 ggtgtcgagg tcggcgaaac gcggcgtcca accgagtgat tcccgcgcgg cgcgtgcatc
     1321 ggccaccagc accggcgggt cgccttcgcg gcgttggccg accaccaccg ggatcggatg
     1381 gcccgtcacc cggcgtaccg ctgcgatcac ctcgcgcacc gaatagccat tgccgttgcc
     1441 gaggttgtag cgcgcgctgc cgccgcccgc cagcagctgc cgcaacgcca gcaggtgggc
     1501 atcggcgagg tcttcgacgt ggatgtagtc gcgcacgcag gtgccgtctg cggtggcgta
     1561 gtcctcgcca tacacggtga tgtgcgggcg gcgtcccgcg gccacctgca ggatcagcgg
     1621 gaccaggtgg gtctccggct ggtggcattc gcccagctcg ccttccgggt cggcgccggc
     1681 ggcattgaag tagcgcaggc agaccgcctt cagtccgtag gcgcggtcga aatcctccag
     1741 catctgctcg acgaaccact tgctgcggcc gtaggggttg atcggtgcct tcggatgcgc
     1801 ctcgtcgatc ggtacgtact gcgggtcgcc gaagatcgcc gcggtggagg agaagatgaa
     1861 acggttgacg ccatgcgcgc gcatgccgtc cagcagcacc tgggtggcgg tgaggttgtt
     1921 gcggtagtac ttcgccggct cggccaccga ttcgccgacc tggatgcagc tggcgaagtg
     1981 catcaccgct gccggcctga cctcgcccag cagcgcatcc acgaaggccg catcgccgat
     2041 gtcgccgacg tgcagcggtg ctccgagcac cgcttcgcga tggccggagg aaagatcgtc
     2101 ggccaccacc gcgtgcaagc cggcgcggcg cagccgcttg accatgtgcg agccgatgta
     2161 gccggcgccg ccgacgacga ggatggaatc gggcatgtag gcgaggagtg acgtggtgcg
     2221 gcgattgtcg cgcatgcggc gtggccggtg cgcgcggcaa tcgtgctgcc gtatccttcg
     2281 cgcgatgacg attggcccca tgactgcccg ccagatgacc gccaccaccg gcctgcgcgt
     2341 cctgcgcgca ccgctgctgg catgggccgc ggtggcgctg gtctcgatct tcctgcacgg
     2401 gccgctgccg ctgtannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncatgg
     2521 gccgcggtgg cgctggtctc gatcttcctg cacgggccgc tgccgctgta ttccacccgc
     2581 agcctggcgg tggcgtggga gatgtgggat gcgggcagct ggctggtgcc gctgttcaat
     2641 ggcgagccgt attcgcacaa gacgccgttg ctgccgtggc tgatccacct cggctggctg
     2701 ctcggcgggg tcagtgacct ctggccgcgc gtgctggtgg tgctgctggg cacgctggcg
     2761 atcgcgcaga ccggcttgct ggcgggccgg ctgtatccgc ggcggccggc attgcccgcg
     2821 ctggcggcat gggcgatggc cggcttctgg tacttcttcc tgttctcgct gcaggtgatg
     2881 tacgaattgc cgctgatggt ggcggtgctc ggcggcctgc tggcgctgtg ccggcgcgac
     2941 ggcgccggct ggaatccgtg gtggccgggc gtggcgttgg cagtcggctt tggcttgctg
     3001 gcgaagggcc cggtggtcct gctgcacctg ggcgtgccgc tgctggcggc gcgctggtgg
     3061 cacccggccg cgcaacgcga tggcgcgcgc ttcgcgaagc gggcgttgct cgcggtgctg
     3121 gccggcatcg gcgtgttcgc actgtggctg ttgccggccc tgaccctggg cgatccggaa
     3181 taccgccagg ccctgttggt cacccagacc gccggccgca tccgcgacag cttcgaccat
     3241 gccgaaccgt ggtggtggta cctgccgctg ctgctggtac tgatggcgcc gtggtggtgg
     3301 tcgcgctggt ggtgggcgca ggtgccagtg ctctggcggg atgccggcaa ccggttcgcg
     3361 tggatctggg tgatcggcgt ggtgctggcc ttcagcgcga tcagcggcaa gaacccgtac
     3421 tacctgctgc cggagttcgc ggggttcgcc atcctgttgg cgctggcggc ggatacgcgg
     3481 ccgcggctgg gcatcgccgc cggggtgggc gtgctgctgc tggcggcgct gctgctgggc
     3541 gcgcgcgtgc tcgccgggca cgtcgagcgg ccgttcccgg aggcgctgct ggacagcctg
     3601 ccgctcgcgg gcggggtgct ggcggtggtc ggcatcggct tgttgcgctg gcgttcgctg
     3661 ccggtgcagg ccggcggcgt gctgctggcg accgccctgc tgcatgcggc gttgacgccg
     3721 ctgatcaagg cgcaatacga catgaccgcg accgcgcagg cgctgggcca ggcgcaacgc
     3781 gcgggcaccc ggatcgcgtt cctcggcgaa taccagttgc agttccattt cgccgggcgc
     3841 ctgcgcgcgc ccattgaagt ggtcgacgaa gccggcgcgc gtgcctgggc gacgcaacac
     3901 ccgcgtgacc tgatcgtggt caacgcccgc gaagactggc gcgcgccggg accgcatccg
     3961 gtgatccagc agcgtttccg ctcgcgctgg gtgcaggtct ggcgggcgtc cgactggcgc
     4021 gcgctgcccg cgcagcagct accactgcag gcggcggcgc gccagctgcg ctcccagccg
     4081 cgctgacagc gcctgcgccg cggccagcgg caggtggtgc aggtgcaacg gcgcgccgaa
     4141 cggcgaggca ccggcggtat ccagcagcag gctggccatg cccagccggc ggtccagcgg
     4201 cgactgcgcc agccgcagcg cctgcacctt gtcgacttcg gcgaagttcc aggtcttcgc
     4261 cagccagccg gtgcgccagg ccaccagccg gccgttgtcg gcataaccgc aggcggcggc
     4321 gatccggcgt gcgcgccaca gctgcaacgg cagcagcaac aacccgagca ggccaatgcc
     4381 gccgaagcgc cagcaccccg cgacgacggc caccagcacc agcagcagcc ccggcatcgc
     4441 gatccgcagc catgcaccgc ggtgcagcgg ttgccagtcc agcgcgtccc aacctgcatc
     4501 gggcaggaag tgcctgatca gcgcgtcgca gtggccgggg ctggcgatcg gcgcgatgtc
     4561 cttcagcgtg tgctgttcct gcgcgccgcg ctggccggcg gcggtgtcca cgcgcaggct
     4621 gcggcggcgg aaccagcggt gcagaacgcc ttcgcgcagc gaccacgcct ggatccggcg
     4681 gcgcctggcg gatgagcgcg aacgcgccag caggccgcgc tccacgctca ggcggccatc
     4741 gtcctcgcgc agggtgaagc cgtggaactg caggatggcg agcagcaccg acagcagccg
     4801 taccagcacc agcgcgacca ccaccaggct gaccgcggcc aggccgatac cgagcgtgct
     4861 gtcgtgcgcg tgctcgccca tgaattcgga gacgcggccg aacagcagcc tgccccagcg
     4921 ctccaccagc acgccgaaca cgtcctcgcc ggtctgcgcc agggcgccga acgcggcggc
     4981 caccaccagc atgccgcggt tgctgatcag gcccagccgc accagttcgc cggtggacag
     5041 ggccagcagc ggtggcgccg cgtccgcctc gccggcggca acgcccgggt tgccgcgcct
     5101 gcgcaccagc gcttccagcg ccagcgcctg gtccagccgc agcacgcgca tctgcgcttc
     5161 cggcttggtc ccgccggccg attccagccg cacctcggcg accccgaaca tccggtgcag
     5221 cagcgactgg tgcaggttga cgttgtggat gcgtgcgaac gggatctcgc gcaggctgcg
     5281 gtgcagccag ccgctgcgga tgcggatgc
//
LOCUS       DAORUK010000143         5230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_19272_length_5230_cov_18.657778, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000143 DAORUK010000000
VERSION     DAORUK010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19272_length_5230_cov_18.657778"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PK818_08825"
     CDS             <1..270
                     /locus_tag="PK818_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HQY82870.1"
                     /translation="LRRAKMYEFLDRLINISLPRVRDFRGVSGRSFDGRGNYNMGVKE
                     QIIFPEIDFDQVDAMRGMDIAITTTAKTDAEAKALLEAFRFPFRN"
     gene            283..588
                     /gene="rpsN"
                     /locus_tag="PK818_08830"
     CDS             283..588
                     /gene="rpsN"
                     /locus_tag="PK818_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005917137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HQY82871.1"
                     /translation="MAKTSMVNREAKRAKLAKQHGAKRDALKKIISSQDASYEEKMDA
                     AVKLQKLPRDSSPSRQTTRCVLTGRPRAVYSKFGLGRNALRKATMNGDVPGLRKASW"
     gene            767..1162
                     /gene="rpsH"
                     /locus_tag="PK818_08835"
     CDS             767..1162
                     /gene="rpsH"
                     /locus_tag="PK818_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HQY82872.1"
                     /translation="MSMTDPIADMLVRIKNAAAVGKQTVKMPSSKTKVAIANVLKDEG
                     YIADARVTQQGAKAELEIALKYYEGKPVIETLKRVSRSGLRQYRGKDALPKVLHGLGV
                     AIISTSKGIMTDSQARQQGVGGEVLCIVA"
     gene            1176..1703
                     /gene="rplF"
                     /locus_tag="PK818_08840"
     CDS             1176..1703
                     /gene="rplF"
                     /locus_tag="PK818_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006450642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HQY82873.1"
                     /translation="MSRVAKKPVALTQGVELNVQPESISVKGPKGTLSVPKPAGIEVR
                     IEDGNALLSANDASLVPLTGTLRAIVANMVHGVSAGFERKLELVGVGYRAAMQGKDLS
                     LSLGFSHPILFPSPEGITISTPTQTEILVQGADKQRVGEVAAKIRGYRPPEPYKGKGV
                     KYAGEVIIRKEAKKA"
     gene            1748..2104
                     /gene="rplR"
                     /locus_tag="PK818_08845"
     CDS             1748..2104
                     /gene="rplR"
                     /locus_tag="PK818_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HQY82874.1"
                     /translation="MSIKNTARLRRAKSTRAHIRNLGVPRLTVLRTGQHLYAQLFTAD
                     GSKVLASASTVQADVMAGLKNGKNAEAAAKVGQVIAERAKAAGFEKVAFDRSGYRYHG
                     RIKALADAAREGGLQF"
     gene            2268..2801
                     /gene="rpsE"
                     /locus_tag="PK818_08850"
     CDS             2268..2801
                     /gene="rpsE"
                     /locus_tag="PK818_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004145411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HQY82875.1"
                     /translation="MAEPRESRGRGRDREEVDDGMIEKLVAVNRVSKTVKGGRQFTFT
                     ALTVVGDGAGKVGFGYGKAREVPVAIQKSMEQARRGMSSVDLNSGTLFHQVKARHGAA
                     SVFMMPASEGTGVIAGGAMRAVLEAVGVKNVLAKATGSRNPINLVRATIRGLESMTSP
                     AKIAAKRGKKLEDVVHG"
     gene            2794..2985
                     /gene="rpmD"
                     /locus_tag="PK818_08855"
     CDS             2794..2985
                     /gene="rpmD"
                     /locus_tag="PK818_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HQY82876.1"
                     /translation="MAKNETGTVTVRLVKGLRGTQSRHRLSVKALGLGKLNSVRVLKD
                     SPQVRGLINQLHYLVKVED"
     gene            2990..3427
                     /gene="rplO"
                     /locus_tag="PK818_08860"
     CDS             2990..3427
                     /gene="rplO"
                     /locus_tag="PK818_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HQY82877.1"
                     /translation="MKMNTLSPAEGARQERKRVGRGIGSGLGKTCGRGHKGSFARAGK
                     GKIKAGFEGGQMPMQRRLPKIGFRSKLANDCAEVLLYKLDTLPAGDVDFAALRAAKLV
                     PSTAKRAKIVVKGEVSKAYVLKGIAATAGAKAAIEAAGGKLAE"
     gene            3435..4787
                     /gene="secY"
                     /locus_tag="PK818_08865"
     CDS             3435..4787
                     /gene="secY"
                     /locus_tag="PK818_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HQY82878.1"
                     /translation="MSRNANALSGLAGAGKFTELRSRLLFVLGALIVYRIGCFIPVPG
                     VNPEAMLQLMESQQGTIVDMFNMFSGGALSRFSLFALNVIPYISASIVMQLMVQILPS
                     LKALQKEGESGRRKITQYSRYGAVFLAVFQAAGIAIALQGQGASGGIPVVYNPGPGFV
                     MTAVVSLTAGTMFLMWMGEQITERGIGNGVSLIIFAGIVAGLPGAVLNTLQQANSGDM
                     QWITVFFILAIVMGFTWFVVFMERGQRRITVNYARRQGGRGAYMNQSSFLPLKLNMSG
                     VIPPIFASSIIMFPATASTWFGQSGNAATSWLQKVSQMLSPGEPLHMILLAALIIGFA
                     FFYTALVFNSQETADNLKKSGALIPGIRPGKATADYVDGVLTRLTLAGSLYLVLVCLL
                     PEIMRTQLGTSFYFGGTSLLIVVVVVMDFIAQIQAHLMSHQYESLLKKANLKGRGGSR
                     "
BASE COUNT          967 a         1710 c         1724 g          829 t
ORIGIN      
        1 ctgcgccgcg ccaagatgta cgagttcctg gaccgcctga tcaacatctc gctgccgcgc
       61 gtgcgcgact tccgtggggt gtcgggccgt tcgttcgacg gtcgtggcaa ctacaacatg
      121 ggcgtgaagg aacagatcat cttcccggag atcgatttcg accaggtcga cgcgatgcgc
      181 ggcatggaca tcgccatcac caccaccgcg aagaccgatg ccgaggccaa ggccctgctc
      241 gaggccttcc gcttcccgtt ccgcaactaa gggcaagaca agatggccaa gacttcaatg
      301 gtgaaccgcg aggccaagcg cgccaagctt gccaagcagc atggcgccaa gcgcgacgcg
      361 ctgaagaaga tcatctccag ccaggatgcg tcctacgagg agaagatgga tgcggcggtc
      421 aagctgcaga agctgccgcg cgattcctcg ccgagccgcc agaccacccg ttgcgtcctg
      481 accggccgcc cgcgcgccgt gtacagcaag ttcggcttgg gccgcaacgc gctgcgcaag
      541 gcgacgatga acggcgacgt cccgggcctg cgcaaggcta gctggtaacc gaaactcctg
      601 ctatattggg cggcttcccg tcctcaaggc aggaacaacc cgaggcccgc agcgatgcgg
      661 gccttcggtc gcgacaagcg ccaccgccgt tccccggctg gcggcgtcca caatccgaac
      721 agattttcgc gaaagcggat atcggtgcta ctcataaggt gaatccatga gcatgactga
      781 tcccatcgcc gacatgctgg tccgcatcaa gaatgcggcg gcggttggca agcagacggt
      841 gaagatgccg tcgtccaaga ccaaggtcgc gatcgccaac gtcctcaagg acgagggtta
      901 catcgccgac gcgcgcgtca cccagcaggg cgccaaggcc gagctggaaa tcgccctgaa
      961 gtactacgag ggcaagccgg tgatcgagac cctgaagcgc gtgtcgcgct cgggactgcg
     1021 ccagtatcgc ggcaaggacg cgctgccgaa ggtcctgcac ggactcggcg tcgccatcat
     1081 ttccacctcc aagggcatca tgaccgactc gcaggcgcgc cagcagggcg tcggtggtga
     1141 agtcctgtgc atcgtggcct aagggagtaa cgaccatgtc ccgagtcgcc aagaagccgg
     1201 ttgccctgac ccagggtgtc gagctcaacg tccagccgga gtcgatcagc gtcaagggcc
     1261 cgaaggggac cctgagcgtg ccgaagcccg ctggaatcga agtcaggatc gaggacggca
     1321 acgcgctgtt gtcggccaat gacgcctcgc tggtgccgct gaccggcacc ctgcgtgcga
     1381 tcgtcgccaa catggtgcac ggcgtgtccg ccgggttcga gcgcaagctc gagctggtcg
     1441 gcgtcggcta ccgcgccgcc atgcagggca aggacctgag cctgtcgctc gggttctcgc
     1501 acccgatcct gttcccgtcg ccagaaggca tcaccatcag caccccgacc cagaccgaaa
     1561 tcctggtgca gggcgcggac aagcagcgcg ttggcgaagt cgccgccaag atccgcggct
     1621 accgcccgcc ggagccctac aagggcaagg gtgtgaagta cgccggcgaa gtcatcatcc
     1681 gcaaggaagc caagaaggcg taatcccccg cgggatgcac gcgctttcag cttcgaggaa
     1741 ccagaccatg agcatcaaga acaccgcccg cctgcgccgc gccaagtcca cccgcgcgca
     1801 catccgcaat ctcggcgtgc cgaggctgac cgtgctgcgc acgggccagc acctgtacgc
     1861 acagctgttc accgccgatg gctccaaggt gctggcatcc gcctccaccg tgcaggcaga
     1921 cgtgatggcc ggcctgaaga atggcaagaa tgccgaagcc gccgccaagg tcggccaggt
     1981 gatcgccgag cgtgccaagg ccgcgggctt cgagaaggtc gcgttcgacc gctcgggcta
     2041 ccgctaccac ggccgcatca aggcgctggc cgatgccgcg cgcgaaggcg gcctgcagtt
     2101 ctgatccacg atgagggcgc agggcttcgt ccccacgccc acatccgctt gccgatgcaa
     2161 ggtgcatcgg gcgtcctggc cggttgcggc catggcggaa catccgcacc acatcccaac
     2221 aacaagcggc gccgccgcaa atcccttcaa cgaaacgaga tatcgaaatg gcagaaccac
     2281 gcgaatcccg cggccgcgga cgcgaccgcg aagaggtcga tgacggcatg atcgaaaagc
     2341 tggtcgcggt caaccgcgtc agcaagaccg tcaagggcgg tcgccagttc accttcaccg
     2401 cgttgacggt ggtgggcgac ggcgccggca aggtcggctt tggctatggc aaggcgcgcg
     2461 aagtgccggt ggccatccag aagtcgatgg agcaggcgcg ccgtggcatg agctcggtcg
     2521 acctcaacag cggcaccctg ttccaccagg tgaaggcccg ccacggtgca gccagcgtgt
     2581 tcatgatgcc ggcctccgaa ggtaccggcg tgatcgccgg cggcgcgatg cgcgcggtgc
     2641 tggaagcggt gggcgtgaag aacgtgctgg ccaaggccac cggttcgcgc aacccgatca
     2701 acctggtgcg cgccaccatc cgcggcctgg agtcgatgac ctcgccggcc aagatcgcgg
     2761 ccaagcgcgg caagaagctg gaggacgtgg tccatggcta agaatgaaac cggaacggtc
     2821 accgttcgcc tggtcaaggg cctgcgcggt acccagtcgc gccatcgcct gtcggtgaag
     2881 gcgctcgggc tgggcaagct gaacagcgtc cgcgtgctga aggacagccc gcaggtgcgt
     2941 ggcctgatca accagctgca ttacctggtc aaggtcgagg attgatccca tgaaaatgaa
     3001 tactttgagc cccgccgagg gcgcccgcca ggaacgcaag cgcgtcggcc gcggcatcgg
     3061 ttccggcctc ggcaagacct gtggccgcgg ccacaagggc tcgttcgcgc gcgccggcaa
     3121 gggcaagatc aaggccgggt tcgaaggcgg ccagatgccc atgcagcgcc gcctgccgaa
     3181 gatcggcttc cgttcgaagc tggccaacga ctgtgcggaa gtgctgctgt acaagctgga
     3241 cacgctgccg gccggtgatg tcgatttcgc cgccctgcgt gccgccaagc tggtgccgag
     3301 caccgccaag cgcgccaaga tcgtggtcaa gggcgaggtc agcaaggcgt acgtgctgaa
     3361 gggcatcgct gcgaccgccg gcgccaaggc ggcgatcgag gccgccggcg gcaagctggc
     3421 ggagtaaccg gcgaatgtcg cgaaacgcga atgcgttgtc gggcctcgcg ggcgcgggca
     3481 agttcactga acttcgctcg cgcctgctgt tcgtcctcgg tgcgttgatc gtctatcgca
     3541 tcggctgctt catcccggtg ccgggcgtga acccggaagc catgctgcag ctgatggaat
     3601 cgcagcaggg caccatcgtg gacatgttca acatgttcag cggcggcgcg ctttcgcggt
     3661 tctcgctgtt cgcgctgaac gtgatcccgt acatctccgc gtcgatcgtg atgcagctga
     3721 tggtgcagat cctgcccagc ctgaaggcgt tgcagaagga aggcgaatcc ggccgtcgca
     3781 agatcaccca gtattcgcgc tacggcgcgg tgttcctggc cgtgttccag gccgccggca
     3841 tcgccatcgc cctgcagggc cagggcgcca gcggcgggat cccggtggtg tacaacccgg
     3901 gccccggctt cgtgatgacc gcggtggtct cgctgaccgc cggcaccatg ttcctgatgt
     3961 ggatgggcga gcagatcacc gagcgcggga tcggcaacgg cgtgtcgctg atcatcttcg
     4021 ccggcatcgt cgccggcctg ccgggcgcgg tgctcaacac cctgcagcag gccaacagcg
     4081 gcgacatgca gtggatcacg gtgttcttca tcctcgccat cgtgatgggc ttcacctggt
     4141 tcgtggtgtt catggagcgc ggccagcgcc gcatcaccgt gaactacgcg cgtcgccagg
     4201 gtggccgcgg cgcgtacatg aaccagagct cgttcctgcc gctcaagctc aacatgtcgg
     4261 gcgtcatccc gccgattttc gcctcgtcga tcatcatgtt cccggccacc gcctcgacgt
     4321 ggttcggcca gtccggcaat gcggcgacca gctggctgca gaaggtgagc cagatgctgt
     4381 cgcccggcga gccgctgcac atgatcctgc tggccgcgct gatcatcggt ttcgcgttct
     4441 tctacaccgc gctggtgttc aattcgcagg aaaccgcgga caacctgaag aagtcgggcg
     4501 cgctgatccc gggcatcagg ccgggcaagg cgaccgcgga ctatgtcgac ggcgtgctga
     4561 cccggctgac gctggcgggt tcgctgtacc tggttctggt ctgcctgttg ccggagatca
     4621 tgcgcaccca gctggggacc tcgttctact tcggcggaac ctcgctgttg atcgtggtgg
     4681 tcgtggtgat ggatttcatc gcgcagatcc aggcgcacct gatgtcgcac cagtacgaga
     4741 gcctgctgaa gaaggcgaac ctgaaggggc gagggggttc gcgctaagcg cagcactgct
     4801 gcgctgaacc gccgagcgcc cggacaggag tccgggcagg gctccacgga gcccgtgaac
     4861 cagcgccatg gatggcaacg aacaaccagc caaagccgac atctgcgcgg cttggcaaat
     4921 gggcgacccg ggcggcagtc tcgcgcacgg gtggccccga accgacccgc gcgccggagt
     4981 agcgcgcggg caggccgggg agcctttccg cgagggaagc ctgccgggta ctgccagttc
     5041 ggggccatcg ccggagcatg ggcacactcc ccatgccggg ttcccgcgcc attccgggcg
     5101 ggggcttcct gaaaacccga gacctcgcta taatttcatg ttcactgcgc cggattgcca
     5161 aatccgccgc ctttacgtta tcggagatcg ccttatggcg cgtattgcgg gcgtcaacct
     5221 gcctgcccag
//
LOCUS       DAORUK010000144         5211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_19391_length_5211_cov_16.870054, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000144 DAORUK010000000
VERSION     DAORUK010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19391_length_5211_cov_16.870054"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..248
                     /locus_tag="PK818_08870"
     tRNA            164..248
                     /locus_tag="PK818_08870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:198..200,aa:Leu,seq:gag)
     gene            complement(321..755)
                     /locus_tag="PK818_08875"
     CDS             complement(321..755)
                     /locus_tag="PK818_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HQY82879.1"
                     /translation="MSDSGFDLGLDDPARAGVFFVTADDIATLDMATRDAGLLARRID
                     LRGCANKPTLLLRLATGLDFPAGSGRNWDAASDRLRDLSWLPAPGYALLFEAASGLRD
                     ADESSFDTLLDVLEEAAADWAQRDIPFWAFLALPEDEFEPAP"
     gene            complement(752..1243)
                     /locus_tag="PK818_08880"
     CDS             complement(752..1243)
                     /locus_tag="PK818_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006377728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HQY82880.1"
                     /translation="MNRSPGAWVTWALLLLVLVGGWLAWPQAQPPQAPAGRDGAPALP
                     RDPSARPLPPAAPSQPPPVAEPATRLPGFLPAEAHRTIALVQRGGPFPHRQDGGVFGN
                     REGRLPRKPRGWYREYTVDTPGLRHRGARRIVTGGDPPREWYYTDDHYDSFRSIAVTG
                     NLQ"
     gene            complement(1249..1497)
                     /locus_tag="PK818_08885"
     CDS             complement(1249..1497)
                     /locus_tag="PK818_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82881.1"
                     /translation="MTQSERRKDQQRAARELRAELNESQRDTLAELERYGWQLKFVRR
                     PIFQPSIPVVFDGDRKSWAVLEQDGSLNEHPPFVIREG"
     gene            1566..2165
                     /locus_tag="PK818_08890"
     CDS             1566..2165
                     /locus_tag="PK818_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HQY82882.1"
                     /translation="MARDPRVDAYIERAAPFAQPILAHVRALVHQACPRVEESIKWGM
                     PHFSHAGAILCGMAGFKRHAAFGFRKHAQVVGEGEPREGMGSYGKIASLADLPPDPVL
                     LAHIRRAVALNEDGGKAPAVRKAVAKPPPQAPADLVAALAQHPAAQATFDAFPPGCRR
                     EYIEWIVEARRPETRAKRLAQALEWMGAGKRRNWKYERC"
     gene            complement(2182..3906)
                     /locus_tag="PK818_08895"
     CDS             complement(2182..3906)
                     /locus_tag="PK818_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HQY82883.1"
                     /translation="MAPAFAASTALTTTAERTGFRQTGRYDEVIALCDAFQQAYPQAV
                     RCFDFGTTPAGRPMKAMAVSTSGALDAKTAQAQGLPVVLAQGGIHAGEIDGKDAGFWL
                     ARDLLQGKVGRGVLDKAVLLFVPVFNADGHENFRAWNRPNQRGPEQMGHRVTAQRYNL
                     NRDYVKADSPEMQAMLGLVNQWDPLAMLDLHVTDGAKFRHDISITGEPTNSGDAGLRE
                     AGNALKDGIIARLAAQGSLPVGFYPSFVEDDDPASGFVHGVSTPRFSNGYFPLRNRLA
                     ILVETHSWRTYPERVRSTYNTVLNALELVAANGTRWLQLARDADARAARLGGQSVPLD
                     YRTTDAVRTIDFLGYEYTRTPSDISGTSMTRYDERKPQVWKIPLRDQIVPGNAIVAPK
                     GGYLVPPEHAVAVARHLLVHGIDFRVLASPLRDAGAQAFVADGVQFSPASVEGHQRTS
                     LTGGWTTQPAQVGPGGLYVPIAQAKARLLVNILEPTAGDSMAAWGDFNNAFERKEYME
                     AYVAEDEARTMLARDPALKAEFERKLREEPGFANSPGARLDFFYRRHPAWDHGTNRYP
                     VWRTDTAH"
     gene            4088..4723
                     /locus_tag="PK818_08900"
     CDS             4088..4723
                     /locus_tag="PK818_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HQY82884.1"
                     /translation="MDMTLYYAPGAASLLVHWLLIELELPHRLQLVDTAAKAQKSPEY
                     LAMNPNGVVPTLVIDGRPQYEAAALAMWLAERHPQARLAPAFDDPRRADHLQWMCNLA
                     NMVQPLFRQWWYPAEPAGDAHADAVRAHCAPRIEAQWQRIDAHLSINGPFLLGDAPTV
                     ADFYLAMLMRWSRNMPKPATEWPQLAALAQRMKARPSFAALYAREGLTEWA"
     gene            4770..>5211
                     /locus_tag="PK818_08905"
     CDS             4770..>5211
                     /locus_tag="PK818_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY82885.1"
                     /translation="MSQTTVADPSARLPRQIPFIIGNEACERFSFYGMRNILVQFLVS
                     SVILAYVPEAERQGVAKDVFHSFVIGVYFFPLLGGWLSDRFFGKYNTVLWFSLVYCAG
                     HACLAMFEDNRTGFYTGLFLIALGSGGIKPLVVSFCGDQFTTANK"
BASE COUNT          765 a         1772 c         1861 g          813 t
ORIGIN      
        1 ccgagcgcgc aggtgccggc cgcaccggca caggcaccgg cggccccgct ggcagcaccg
       61 gattacaccc agccgccggc gtccgcaccc gaacccgcgc ccgcgcccgc gccgggcagc
      121 tgatcggctt tacaatgttg tggcggcgcg agccgcatca cctgcccagg tggcggaatt
      181 ggtagacgca ctaccttgag gtggtagcga cttaggtcgt aggggttcga gtcccctctt
      241 gggcaccatt tacggaaacc cgcttcggcg ggttttttgt tggagtcggg gtcccaagcg
      301 gggacccgga cccggcaggg tcacggtgcc ggttcgaatt cgtcttccgg cagggcgagg
      361 aaggcccaga acgggatgtc gcgttgcgcc cagtcagccg ccgcttcctc gagcacgtcc
      421 agcagggtgt cgaagctgga ttcgtcggcg tcgcgcaaac cgctggcagc ttcgaacaac
      481 agggcgtagc cgggcgcggg cagccacgac aggtcgcgca ggcggtcgct cgcggcgtcc
      541 cagttgcgtc cgctgccggc ggggaagtcc agcccggtgg ccagccgcaa cagcagggtc
      601 ggcttgttcg cgcagccgcg caggtcgatg cggcgggcca gcaggccggc gtcgcgggtg
      661 gccatgtcga gggtggcgat gtcgtcggcg gtgacgaaga acacgccggc gcgcgccggg
      721 tcgtccaggc ccaggtcgaa gccggaatcg ctcactgcag gttccccgtg accgcaatgc
      781 tgcggaagct gtcgtaatgg tcgtccgtgt agtaccactc gcgcggcggg tcgccgccag
      841 tgacgatccg gcgcgcgccg cgatggcgca gccccggcgt gtccacggtg tactcgcgat
      901 accagccgcg cggcttgcgc ggcagccggc cctcgcggtt gccgaacacg ccgccgtcct
      961 gccggtgcgg gaacggaccg ccgcgctgga ccagggcaat ggtccggtgc gcttccgcgg
     1021 gcaggaaacc gggcagtcgg gtggcgggtt cggccacggg tggtggctgc gacggcgcag
     1081 ccggcggcaa tggccgcgcg ctcgggtcgc gcggcagcgc gggcgcgcca tcgcggccgg
     1141 ccggcgcctg cgggggctgc gcttgcggcc atgccagcca gccgccgacc agcaccagca
     1201 acaacagcgc ccacgtgacc cacgcgccgg gactgcggtt caccaccatc aaccttcccg
     1261 gatgacgaag ggcgggtgct cgttgaggct gccgtcctgc tcaagcaccg cccatgactt
     1321 gcggtcgccg tcgaacacga ccgggatcga cggctggaag atcggcctgc gcacgaactt
     1381 caattgccag ccgtagcgtt ccagttcggc aagcgtgtcg cgctgcgact cgttcagttc
     1441 ggcgcgcagt tcgcgcgcgg cgcgttgctg gtccttgcgg cgttcggatt gggtcatgcg
     1501 cggtcccctg tgcggctacg agcaatggcg gcagaatggc cccgtccccc gatgcgtgca
     1561 agcacatggc ccgcgatccc cgcgtcgatg cctacatcga gcgtgccgcc ccgttcgcgc
     1621 aaccgatcct cgcccacgtg cgcgcgctgg tgcaccaggc ctgtccgcgg gtggaggaat
     1681 cgatcaagtg gggcatgccc catttcagcc atgccggcgc catcctgtgc gggatggcgg
     1741 ggttcaagcg gcatgccgcg ttcgggttcc ggaagcacgc gcaagtggtg ggcgagggcg
     1801 agccgcgcga gggaatgggc agctacggca agatcgcgtc gctggcggac ctgccgccgg
     1861 atccggtcct gcttgcgcac atccgccggg cggtggcgct caacgaagat ggcggcaagg
     1921 cgccggcggt gcgcaaggcc gtggcgaagc cgccaccgca agcgccggcc gacctggtcg
     1981 ccgcgctcgc gcagcacccg gcggcgcagg cgaccttcga tgcgttcccg cccggctgca
     2041 ggcgcgagta catcgagtgg atcgtcgagg cgaggcgccc ggaaacccgc gcgaagcgac
     2101 tggcgcaggc gcttgaatgg atgggcgcgg gcaagcgccg caactggaag tacgagcgct
     2161 gctgaagccg tccagcggcg gtcaatgggc ggtatcggtg cgccacaccg gatagcggtt
     2221 ggtgccgtgg tcccaggccg ggtgccggcg gtagaagaag tccaggcgcg cgccggggct
     2281 gttcgcgaag ccaggttcct cccgcagctt gcgctcgaac tccgccttca gcgccgggtc
     2341 ccgggccagc atcgtgcggg cttcgtcctc ggccacgtac gcctccatgt attccttgcg
     2401 ctcgaaggcg ttgttgaagt cgccccatgc ggccatcgaa tccccggcgg tcggctccag
     2461 gatgttcacc agcagccgcg ccttggcctg cgcgatcggc acgtacaggc cgcctgggcc
     2521 gacctgcgcc ggctgcgttg tccagccacc cgtcaggctc gtccgctggt ggccttccac
     2581 cgacgccggc gagaactgca cgccatcggc gacgaaggct tgcgcgccgg catcgcgcag
     2641 cggcgaggcc agtacccgga agtcgatgcc gtgcaccagc aagtgccttg ccaccgcgac
     2701 cgcatgttcc ggcggcacca ggtagccacc cttcggcgcg acgatcgcgt tgcccggcac
     2761 gatctggtcg cgcaggggga tcttccacac ctgcggcttg cgctcgtcgt agcgggtcat
     2821 gctggtgccc gagatatccg atggcgtgcg ggtgtattcg tagccgagga aatcgatggt
     2881 gcggacggca tcggtggtcc tgtagtccag cggcaccgac tggccgccca gccgcgctgc
     2941 gcgcgcatcg gcgtcgcgcg ccagttgcag ccagcgggtg ccgttcgccg cgaccagctc
     3001 cagtgcgttg agcacggtgt tgtaggtgct gcgcacgcgt tccggatagg tgcgccagga
     3061 gtgggtctcg accaggatcg caagccggtt gcggagcggg aaatagccgt tggagaagcg
     3121 cggcgtgctc accccatgca cgaagccgga ggccggatcg tcgtcctcga cgaagctcgg
     3181 gtagaagccg accggcagcg aaccttgcgc ggcgagcctg gcgatgatgc cgtccttcag
     3241 cgcattgccg gcctcgcgca ggccggcgtc gccggaattg gtgggttcgc cggtgatcga
     3301 gatgtcgtgc cggaacttgg cgccgtcggt gacgtgcagg tccagcatcg ccagcgggtc
     3361 ccactggttg accagcccca gcatcgcctg catttcgggg ctgtcggcct ttacgtaatc
     3421 gcggttgagg ttgtagcgct gcgcggtgac gcggtggccc atctgctcgg ggccgcgctg
     3481 gttgggtcgg ttccaggcgc ggaagttctc gtggccgtcg gcgttgaaca ccggcacgaa
     3541 cagcagcacc gccttgtcca gcacgccgcg gccgaccttg ccctgcagca ggtcgcgggc
     3601 cagccagaag ccggcgtcct tgccgtcgat ctcgccggcg tggatgccgc cctgcgccag
     3661 caccactggc agtccttgcg cctgtgcggt cttcgcatcc agcgcgccgc tggtggagac
     3721 cgccatcgcc ttcatcggcc tgcccgcggg cgtggtgccg aagtcgaagc agcgcaccgc
     3781 ttgcgggtac gcctgctgga acgcatcgca aagtgcgatg acctcatcgt agcggccggt
     3841 ctgccggaac cccgtgcgtt cggcggtggt ggtgagcgcc gtgctggcgg cgaaggcggg
     3901 cgccatcccc agtgacagcg ggcaggcaag cagggcggca gcgagcaagg tacggcgcat
     3961 cggcgggtct ccggtggatc gcccgatggt aaccggcacc agcgtcgggc cggccctgcc
     4021 catggtcatg ccgcggcacc ccgacggggc gtgcgaaaat ccgccaccac ccgcgcgagg
     4081 cctggccatg gacatgacgc tctactacgc ccccggtgcc gccagcctgc tcgtgcactg
     4141 gctgctgatc gaactcgagc tgccgcaccg gttgcagctg gtggacaccg cggcgaaggc
     4201 gcagaagtcg cccgagtacc tggcgatgaa cccgaacggg gtggtgccga ccctggtcat
     4261 cgacggccgg ccgcagtacg aggccgcggc actggcgatg tggctggcag aacgccatcc
     4321 gcaggcgcgg ctggcgcctg cattcgacga tccgcggcgt gccgaccacc tgcagtggat
     4381 gtgcaacctc gccaacatgg tgcagccgct gttccggcaa tggtggtatc cggcggagcc
     4441 ggccggcgac gcgcatgcgg atgcggtgcg tgcgcattgc gcgccgcgga tcgaggccca
     4501 gtggcagcgc atcgacgccc acctttcgat caacggcccc ttcctgttgg gcgatgcgcc
     4561 cacggtcgcg gacttctacc tggccatgct gatgcgctgg tcgcgcaaca tgccgaagcc
     4621 ggcaacggaa tggccgcagc tggcggccct ggcgcagcgg atgaaggcgc ggccgtcgtt
     4681 cgcggccctg tacgcacgcg aaggactgac cgaatgggcc tgatcgacgg atcgggtagg
     4741 cttgcgcaaa ccgaaggacc cgccaacgca tgagccagac caccgtcgcc gacccgagcg
     4801 cgcgcctgcc ccggcagatc cccttcatca tcggcaacga ggcctgcgag cgcttcagct
     4861 tctacgggat gcgcaacatc ctggtgcagt tcctggtcag cagcgtgatc ctggcctacg
     4921 tgccggaggc ggagcgccag ggcgtcgcca aggacgtgtt ccacagcttc gtgatcggcg
     4981 tgtatttctt cccgttgctc ggtggctggc tgtccgaccg cttcttcggc aagtacaaca
     5041 cggtgctgtg gttctcgctg gtctattgcg cgggccacgc ctgccttgcg atgttcgagg
     5101 acaaccgaac cggcttctac accggcctgt tcctgatcgc gctgggcagc ggcgggatca
     5161 agccgctggt ggtcagcttc tgcggcgacc agttcaccac cgccaacaag c
//
LOCUS       DAORUK010000145         5205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_19422_length_5205_cov_19.497282, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000145 DAORUK010000000
VERSION     DAORUK010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19422_length_5205_cov_19.497282"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /gene="hemW"
                     /locus_tag="PK818_08910"
     CDS             <1..1125
                     /gene="hemW"
                     /locus_tag="PK818_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014504451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HQY82886.1"
                     /translation="YVHLPWCVRKCPYCDFNSHEQRGALQFEAYVDALIADLDHDLPL
                     AWGRTVQTVFFGGGTPSLFPPAAIDRFLQLASARLRFAPNAEVTLETNPGTVEHGPFA
                     GYRTAGVNRLSFGVQSFDDGCLQRLGRIHSSGDAERAVKAAQDAGFDNCNLDLMYALP
                     QQTLAMAEHDVERAIALQPTHVSHYQLTLEPNTVFAARTPPGIPDEDGAWDMQEACQA
                     RLAGAGFAQYEVSAYAREGRQCAHNLNYWRFGDYLGIGAGAHAKLTLGASQQVLRRWK
                     VRHPGEYLLKAGTAAAVGGDDFLDAQRLPFDFMLNALRLNDGVPMAMFEARTGLPRAA
                     IADTLAVARDRGWLEAEDDWLRPTELGRRFGNDVIGLFLH"
     gene            1251..3473
                     /locus_tag="PK818_08915"
     CDS             1251..3473
                     /locus_tag="PK818_08915"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HQY82887.1"
                     /translation="MTLPRRVRRALENQLQTANADLNRQLQVVLQETGLELGRQAEHA
                     SDHNVQASLFESIRKLRGAEAGFSDQFARSLEATLAALRAPRVSRRLEELALPAQGLS
                     LVEDVEMDEGAVLGSIAARGDSRNSLALQLMGYRFGVLAGGPAFDAEHLPLGPHALCH
                     ALRSACDWLMLSMDARLLLYRQFEKVAMAHYPALLETLNARLVGDGILPHLSFVPVRM
                     RAATPGIGRGSESAAAVATRSAPTPPAAAAADASPPPASIPPVDGAGAPRTADAPRES
                     AFAALQGLLARRRKLLAKLRPGGAEERVREPLSRDEVLASLRRLRGNGGKHGNLADIR
                     QTLLAQARQMHGHGVTLTDADGDGFELFSLFLAQLQRELRAGSPGHALVDSLKLPLLQ
                     LALRDHRFFTDAAHPARMLLDAVSLAGARWLGEDDLDPQWLGLLQRAVGTVHEDPEAG
                     NDTFVAANHALQGGLQAAARKNEMSERRQVEAARGREKLGLARQRATNEIQRLVAGRP
                     LPRFHLILLEQAWADVLSLALLRGGEDSDGWRDLREATAAIVEASATPIQSTRDPAFV
                     AKLQDALGQVGYHAEDAGAIARQLANGRTEDADLASRTELIVQLRARARLGEENVART
                     AVAQAPHNPAEQAAHATLAIHADGCWVDLFDPADDSVVRRRLAWLSPRSGQALLLNRR
                     GLRVAGDESLDGLARKLANSRLQLVESDIHPAELAWQATMANLSRIAGDATQETAHGR
                     "
     gene            3463..3807
                     /locus_tag="PK818_08920"
     CDS             3463..3807
                     /locus_tag="PK818_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQY82888.1"
                     /translation="MGAESRRMPRRTVSGLNEVVDVMTEETIGHLGNLSVGGMLLIAN
                     TRLPEDALCQLRFTLPESDGHSIEVGAHVLWSDDASAPGQSWAGLRFLGLAPQVTRSL
                     REWVGRSGSDAR"
     gene            3882..5204
                     /gene="pepQ"
                     /locus_tag="PK818_08925"
     CDS             3882..5204
                     /gene="pepQ"
                     /locus_tag="PK818_08925"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HQY82889.1"
                     /translation="MSATTLASLYPSHLATLQARAAEALARGGFDHLLVPSGNLHYQL
                     FDDRDYPYAVNPQFKAWLPLTRAPGSWLVVTPGAKPKLLFLQPHDYWHVVPAAPGGHW
                     VEHFDIVVIRTPEEATRHLPKDASRCAILGEPQGMTGDHLPNNPQAVLAYLEYHRAFK
                     TPYEIAMMREASRIGVRAHRAAERAFRAGSSEFGIHLAYCQAAGQDANDLPYGNIVCL
                     NEHAAVLHYTDRDRIPPTQARSFLIDAGASFGGYASDITRTYSAQADEFAALIEAVDA
                     AQLKMCDAVRAGTDYRQLHLDAHLALAGILKDFGVITVSPEAAVQTGVSRAFFPHGIG
                     HGIGLQVHDVAGFAESDHGGTIPKPDGHPYLRLTRVLEPGMVVTIEPGLYFIDMLLDE
                     LKAKGQGDAVDWDRVDAFRPCGGIRIEDDVVCTEGVPLNLTREAFATA"
BASE COUNT          824 a         1875 c         1769 g          737 t
ORIGIN      
        1 tacgtgcacc tgccgtggtg cgtgcgcaag tgcccgtact gcgatttcaa ctcgcacgaa
       61 caacgcgggg cgctgcagtt cgaggcgtat gtcgatgcat tgatcgccga cctcgaccac
      121 gacctgccgc tggcatgggg ccgcaccgtg cagaccgtgt tcttcggcgg cggcacgccc
      181 tcgctgttcc cgccggcggc gatcgaccgt ttcctgcaac tggccagcgc gcgcctgcgc
      241 ttcgccccga acgccgaggt caccctggaa accaatccgg gcacggtcga gcacggcccg
      301 ttcgccggct atcgcacggc cggggtgaac cgcctgagct tcggcgtgca gagcttcgac
      361 gatggctgcc tgcagcgcct cggccgcatc cattcaagcg gcgacgcgga gcgcgcggtg
      421 aaggccgcgc aggatgccgg cttcgacaac tgcaacctcg acctgatgta tgcgctgccg
      481 caacagacgc tggcgatggc ggagcacgac gtcgaacgcg cgatcgcgct gcagccgacc
      541 cacgtcagcc attaccagct gacgctggag ccgaacacgg tattcgccgc gcggaccccg
      601 cccgggatcc ccgacgagga cggcgcatgg gacatgcagg aggcctgcca ggcacgcctg
      661 gccggggccg gcttcgccca gtacgaggtc agcgcgtatg cgcgcgaagg ccggcaatgc
      721 gcgcacaacc tgaactactg gcggttcggt gactacctcg ggatcggcgc cggcgcgcac
      781 gcgaagctca cgctgggcgc cagccagcag gtcctgaggc gctggaaggt caggcatccc
      841 ggcgagtacc tgctgaaggc cggcaccgcc gccgcggtcg gcggcgacga cttcctcgac
      901 gcccaacggc tgccattcga cttcatgctg aacgcgctgc gcctgaacga cggcgtgccg
      961 atggcgatgt tcgaggcccg caccggcctg ccgcgcgcgg ccatcgccga taccctggcg
     1021 gtcgcccgcg accgcggctg gctggaagcc gaggacgact ggctgcgtcc gaccgagctt
     1081 ggccgccgct tcggcaatga cgtgattggc ctgttcctgc actgaatgcc gtggattgag
     1141 gcattgccgc ttcgcgcata ccattgatcg gattgccgac gcgttggggg acgccgatgg
     1201 ttgctaacgg ttccacacag ctctcagcca caccgaagac gctggccgcg atgacgctgc
     1261 cgcggcgggt acggcgtgcg ctggaaaacc agttgcagac cgccaacgcc gacctcaacc
     1321 ggcaactgca ggtcgtactg caggaaaccg ggctggagct gggccggcag gcggaacacg
     1381 cgagcgacca caacgtgcag gcgtcgctgt tcgaatccat ccgcaagctg cgcggcgccg
     1441 aggcgggctt ctcggatcag ttcgcgcgga gcctcgaggc gacccttgcg gccctgcgcg
     1501 caccgcgcgt gtcgcgccgg ctggaggaac tggcattgcc cgcgcagggc ctgagcctgg
     1561 tcgaggacgt ggagatggac gaaggcgcgg tgctcggcag catcgcggcg cgcggcgatt
     1621 cgcgcaacag cctggcactg caattgatgg ggtatcgctt cggggtgctt gccggcggtc
     1681 cggcgttcga cgccgagcac cttccgctgg gcccgcatgc gctgtgccat gcgctgcgca
     1741 gcgcctgcga ctggctgatg ctgtcgatgg atgcgcgcct gctgctgtac cgccagttcg
     1801 aaaaggtggc gatggcgcat tacccggcgc tgctggagac gctcaatgca cgcctggtcg
     1861 gcgacggcat cctgccgcac ctgagcttcg tcccggtgcg catgcgcgcc gccacgcccg
     1921 ggatcgggcg cggcagcgaa tcggccgcgg ccgtggccac gcgttcggct cccacgcctc
     1981 ctgcagcggc ggcggccgat gcatcgccgc cgccggcgtc gatcccgccg gtcgatggtg
     2041 cgggcgcgcc acgcacggcc gatgcgccgc gcgaaagcgc ctttgccgcc ctgcaggggc
     2101 tgcttgcacg ccgccgcaag ctgctggcga agctgcgccc gggcggcgcc gaggaacgcg
     2161 tgcgcgagcc gctgtcccgc gacgaagttc tcgcatcgct gcgccgcctg cggggcaacg
     2221 gcggcaagca cggcaacctg gccgacatcc gccagaccct gctggcgcag gcgcggcaga
     2281 tgcacggcca tggcgtcacc ctcaccgatg ccgacggcga cggcttcgag ctgttcagcc
     2341 tgttcctggc ccagctgcaa cgcgaactgc gagccggcag ccccggccat gccctggtcg
     2401 atagcctcaa gctcccgctg ctgcagctgg ccttgcgcga ccaccgcttc ttcaccgatg
     2461 ccgcgcatcc ggcgcggatg ctgctggatg cggtgtcgct ggccggtgcg cgctggctgg
     2521 gcgaggatga cctcgaccca caatggcttg gcctgctgca acgcgcggtc ggtaccgtgc
     2581 atgaagaccc ggaagccgga aacgacacct tcgtcgccgc caaccatgca ctgcagggcg
     2641 gcctgcaggc cgcggcacgc aagaacgaga tgagcgagcg ccgccaggtc gaggccgcac
     2701 gcgggcgcga gaagctgggc ctcgcacggc agcgagccac caacgagatc cagcgcctgg
     2761 tcgccgggcg cccgctgccg cggttccacc tgatcctgct ggagcaggcc tgggccgacg
     2821 tgctgtcgct cgcgctgttg cgtggcggcg aggattccga tggctggcgc gacttgcgcg
     2881 aggcaaccgc cgccatcgtc gaggccagcg ccaccccgat ccagagcacc cgggaccccg
     2941 ccttcgtggc caagctgcag gacgcgctcg ggcaggtcgg ctaccacgcc gaggatgcag
     3001 gtgcgattgc ccgccagctg gccaacggcc gtaccgagga tgcggacctg gcgtcgcgca
     3061 ccgaactgat cgtgcagctg cgcgcgcgcg cgcgcctggg cgaggaaaac gtggcccgca
     3121 ccgccgttgc acaggcaccg cacaacccgg ccgagcaagc cgcgcatgca acgctcgcaa
     3181 tccatgcgga cggttgctgg gtcgacctgt tcgatcccgc cgacgacagc gtggtccgcc
     3241 gccgcctggc ctggctgagt ccgcgcagcg gacaggccct gctgctgaac cgccgcggcc
     3301 tgcgggtcgc gggcgatgaa agcctggatg gcctcgcccg caagcttgcc aacagccgcc
     3361 tgcaactggt cgagtccgac atccacccgg ccgaactcgc atggcaggcg acgatggcca
     3421 acctttcgcg catcgccggc gacgccaccc aggagaccgc acatgggcgc tgaatcgagg
     3481 cgcatgccgc gacgaaccgt cagcggcctg aacgaagtcg tggacgtgat gaccgaggaa
     3541 accatcggcc acctcggcaa cctgtcggtc ggcgggatgc tgctgatcgc caacacccgg
     3601 ctgccggagg atgcgctttg ccagctccgc ttcaccttgc cggagagcga cgggcacagc
     3661 atcgaggtcg gcgcgcatgt gctgtggagc gatgacgcca gcgcccccgg ccagtcctgg
     3721 gccgggctgc gcttcctcgg gctggccccg caggtcaccc gcagcctgcg cgagtgggtc
     3781 ggccgcagcg gcagcgacgc gcgctgacgc atcggcgcgc cggcacggcg gttgcaggcg
     3841 caatcggtca aaatcgcggt tcccgaccca cggaacgcgc catgtccgcg accaccctcg
     3901 ccagcctcta cccctcccat ctcgccaccc tgcaggcgcg cgccgccgag gcgctggccc
     3961 gcggcggatt cgaccacctg ctggtgccca gcggcaacct gcactaccag ctgttcgacg
     4021 accgcgacta cccgtatgcg gtgaacccgc aattcaaggc atggttgccg ctgacccgcg
     4081 caccgggcag ctggctggtg gtgacccccg gcgcgaagcc gaagctgctg ttcctgcaac
     4141 cgcacgacta ctggcacgtg gtgccggccg cacccggcgg gcattgggtc gaacatttcg
     4201 acatcgtcgt catccgcacg ccggaagaag ccacccggca ccttccgaag gatgcatcgc
     4261 gctgcgcgat cctcggcgag ccgcagggca tgaccggcga ccacctgccg aacaatccgc
     4321 aagcagtgct cgcttacctg gaataccacc gcgcattcaa gacgccgtat gagatcgcga
     4381 tgatgcgcga ggccagccgc atcggcgtgc gcgcgcatcg tgccgccgag cgcgcgttcc
     4441 gtgccggctc cagcgaattc ggcatccacc tggcctactg ccaggccgcc ggccaggacg
     4501 ccaacgacct gccctacggc aacatcgtct gcctcaacga gcacgccgcg gtgctgcact
     4561 acaccgaccg cgaccgcatc ccgccgacgc aggcgcgcag cttcctgatc gacgccggcg
     4621 ccagcttcgg cggctatgcc agcgacatca cccgcaccta ttccgcgcag gccgacgaat
     4681 tcgccgcgct gatcgaggcg gtcgacgccg cgcaattgaa gatgtgcgac gcggtgcgcg
     4741 ccggcaccga ctaccgccag ttgcacctcg acgcgcacct cgcgctggcc gggatcctca
     4801 aggacttcgg cgtgatcacg gtaagcccgg aagcggcggt gcagaccggc gtcagccgcg
     4861 cgttcttccc gcacggcatc ggccacggca tcggcctgca ggtgcatgac gtggccggct
     4921 tcgccgagtc cgaccatggc ggcacgatcc cgaagccgga cggccatccc tacctgcgcc
     4981 tgacccgcgt gctggagcct ggcatggtgg tcaccatcga gccgggcctc tacttcatcg
     5041 acatgctgct ggacgagctc aaggccaagg gccagggcga tgcggtggac tgggatcgcg
     5101 tggacgcgtt caggccctgt ggcgggatcc gcatcgagga cgacgtggta tgcaccgagg
     5161 gcgtgccgct caacctgacg cgggaagcgt tcgcgacggc ttgaa
//
LOCUS       DAORUK010000146         5154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_19740_length_5154_cov_17.549912, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000146 DAORUK010000000
VERSION     DAORUK010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19740_length_5154_cov_17.549912"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PK818_08930"
     CDS             complement(<1..565)
                     /locus_tag="PK818_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY82890.1"
                     /translation="MQTLDGVVIGDAPLIRVRGLVNRFGDQLVHDGVDLDVRRGEILG
                     VVGGSGTGKSVLMRSILALRSPEAGEIRLLGVDGRSRDPVQRRHIERNTGVLFQDGAL
                     FSSLTVGENVQVPLKEYYPELPDSLRYELALLKIKLAGLPADALDKLPSQLSGGMRKR
                     AGLARALALDPPLLFLDEPTAGLDPIGA"
     gene            complement(583..1704)
                     /locus_tag="PK818_08935"
     CDS             complement(583..1704)
                     /locus_tag="PK818_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HQY82891.1"
                     /translation="MSVAPNHHPPAAAVDPAEPGTLRLSGRWTLRFANEIGEALRASP
                     DGIVRVDARAVERIDTLGVLQLLRHADRRKLDFDHFEFRDDQKSLIAAIEDVHDERPK
                     PERDTGFKAALGRLGHAVVDNGREVAALVAFLGELLVKLLRLFKEPSRFRLTSTVHHM
                     EQVGLDSAPLVALLSFMVGAVIAFLGAMVLRDFGATIFVVELVNAAFLREFGVLLTAI
                     LLAGRTASAFTAQIGMMVNREEVDAIRVLGLDPVDLLVIPRVLALLAMLPLLTFIAMI
                     AGLAGGMAVGAFTLDIPAVAYFARMQEMMETRHFVVGMVKAPVFAVLIALIGCLEGLQ
                     VAGTAQSLGERTTSSVVQSISMVIVIDALAALWFMEMDY"
     gene            1790..3040
                     /locus_tag="PK818_08940"
     CDS             1790..3040
                     /locus_tag="PK818_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HQY82892.1"
                     /translation="MRPIDAPPEAGYAERIAFVVELGGHLHAYGTTAQRLEGAVEAVA
                     KRLGLECEPWVNPTGMILAFSDPARPAGESDTTRVIRLGLGDTDLYKLCEADRIAEDV
                     MAGRTQIADGRQALRALSRGTGWRGRGMQLLGFALASAAVAGLLRLPWLDIATAGVIG
                     LLIGLVDIAAQQRPRLQEAADAISGLVAGTVAILVSNLVAPLNLNTVIIASLIVLLPG
                     MSLTNAVNELTSRHLVSGTARFAGALTTILKLTIGTAIALTVANLAGLQPEVRALRPQ
                     PDVVMWAALLVGAYAFAVLFRAHRRDYPLVMLAAISGYLISRHGGEAFGPQAGVFLAA
                     LVTTAAGNAYARWMNRPGALIRLPGIIMLVPGSVALRGVISLVQAQDLGAGQEAAMAA
                     LNTLMALLAGLLFGNLLVSARRNL"
     gene            complement(3133..3507)
                     /locus_tag="PK818_08945"
     CDS             complement(3133..3507)
                     /locus_tag="PK818_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010480700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HQY82893.1"
                     /translation="MSVNIEGLSSKELAELISRANQRKKVLAKRKPAAQVKAAVSKLL
                     KASGWTFAELYGAAGAARPAAAAAPKPARKSTKGRTLGKVAPKYRNPANAKDTWAGRG
                     KQPKWLAAETAKGRKLEEFLIK"
     gene            complement(3605..>5154)
                     /locus_tag="PK818_08950"
     CDS             complement(3605..>5154)
                     /locus_tag="PK818_08950"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HQY82894.1"
                     /translation="GIVREEMNRAGAIEMAMPSIQPKELWDETGRWAKFGPQLLKIRD
                     RKDQDYCFTPTAEEAVTDFFRQEVASYKQLPVNFYQVSTKFRDEIRPRFGVMRAREFI
                     MKDAYSFHIDEDSLAAEYRNMYDTYSRIFARLGLKFRAVFADTGAIGGSASHEFHVLA
                     DSGEDAIAFSDGSDYAANVELAEAVSPGERPAPSEALREIDTPTQKTCEDVAALMGIP
                     LARTAKSVALVGTDADGKDQLVLALVRGDHMVNEIKLAKLPGLAEYRLASEAEIAESL
                     GSEPGFLGPLHARKPIRVIADRSVAALADFVVGANRPGFHIAGVNWGRDLPEPEIADI
                     RNVVAGDASPDGNGTLGLARGIEVGHVFALGRKYSEAMDATVLDTNGKAVHPAMGCYG
                     IGVSRIVAAAIEQNHDDNGILWPAAMAPWQVAVCVINPKNDPNVAAAADALYAELNAR
                     GLDAVLDDRGLRAGSMFADIELIGIPHRVVVSGRGLEAGTFEYRARNAAEAEQLDRES
                     LLHRLAG"
BASE COUNT          738 a         1803 c         1784 g          829 t
ORIGIN      
        1 ccgcgccgat cgggtcgagg ccggcggtcg gttcgtcgag gaacagcagc ggcgggtcca
       61 gcgccagcgc gcgcgccagc ccggcgcgct tgcgcatgcc gccggacagt tgcgagggca
      121 gcttgtcgag ggcgtcggcg ggcagcccgg ccagcttgat cttcagcagc gccagttcgt
      181 agcgcagcga atccggcagc tccgggtagt actccttcag cggcacctgc acgttttcgc
      241 ccacggtcag cgaggagaac agcgcgccgt cctggaacag caccccggtg ttgcgctcga
      301 tgtggcggcg ctgcaccgga tcgcgcgagc gcccgtccac ccccagcagc cggatctcgc
      361 cggcctccgg gctgcgcagg gccaggatcg agcgcatcaa caccgacttg ccggtgccgg
      421 agccgccgac cacgccgagg atctcgccgc ggcggacgtc gaggtcgacg ccgtcgtgca
      481 ccagctggtc gccgaagcgg ttgaccagcc cgcgcacccg gatcagcggc gcatcgccga
      541 tcaccacccc atcgagggtc tgcatcgtgg cggcgggcat gctcagtagt ccatttccat
      601 gaaccacagc gccgccagcg catcgatcac gatcaccatc gagatcgact gcaccacgct
      661 ggaggtcgtg cgttcgccca gcgactgcgc ggtgccggcg acctgcaggc cctccaggca
      721 gccgatcagc gcgatcagca ccgcgaacac cggcgccttc accatcccga ccacgaaatg
      781 gcgggtctcc atcatttcct gcatccgcgc gaagtaggcg accgcgggga tgtcgagggt
      841 gaacgcgccc accgccatgc caccggcgag gccggcgatc atcgcgatga aggtcagcag
      901 cggcagcatc gccagcagcg ccagcacgcg cgggatcacc agcaggtcga ccggatcgag
      961 gccgagcacc cggatcgcgt cgacttcctc gcggttgacc atcatcccga tctgcgcggt
     1021 gaacgcgctg gcggtgcggc cggccagcag gatcgcggtc agcagcacgc cgaactcgcg
     1081 caggaacgcg gcgttgacca gctcgaccac gaagatggtg gcgccgaagt cgcgcagcac
     1141 catcgccccg aggaaggcga tcaccgcgcc gaccatgaac gacagcagcg ccaccagcgg
     1201 cgccgagtca agtcccacct gctccatgtg gtggacggtg gaggtgaggc ggaagcggct
     1261 cggttccttg aacaggcgca gcagcttgac cagcagctcg ccgaggaacg cgaccagcgc
     1321 cgcgacctcg cggccgttgt cgaccaccgc atggccaagc cgccccagcg ccgccttgaa
     1381 gccggtgtcg cgttcgggct tggggcgctc gtcgtggacg tcctcgatcg cggcgatcag
     1441 cgatttctgg tcgtcgcgga actcgaagtg gtcgaagtcg agcttgcggc ggtccgcgtg
     1501 gcgcagcagc tgcagcacgc cgagggtatc gatccgctcc accgcgcgcg catccacgcg
     1561 gacgatgccg tccgggctcg cgcgcaacgc ttcgccgatc tcgttggcga agcgcagcgt
     1621 ccagcgcccg gacaggcgca gcgtccccgg ctccgcggga tcgacggcgg cggcaggcgg
     1681 atggtggttg ggggcgacgc tcatgcgggc tcgggttcgg cgtgcgccaa gcttacgcaa
     1741 ctccatgcat ccggtgtgaa ccaccggccg cgcgtgcgat ccttgccgga tgcgacccat
     1801 cgacgccccg ccagaggccg gctacgccga gcgcatcgcc ttcgtggtcg agctgggcgg
     1861 ccacctgcac gcgtacggca ccaccgcgca gcggctggaa ggcgcggtcg aggcggtggc
     1921 caagcggctc gggctggagt gcgagccgtg ggtgaatccc accggcatga tcctggcctt
     1981 cagcgacccc gcgcgcccgg ccggcgaatc cgataccacc cgggtgatcc gcctgggact
     2041 tggcgatacc gacctctaca aattgtgcga ggccgaccgc atcgccgaag acgtgatggc
     2101 cgggcgcacc cagatcgccg acggccgcca ggcgttgcgc gcgctgtcgc gcggcaccgg
     2161 ctggcgcggg cgcggcatgc agctgctggg cttcgcgctg gcgtcggcgg cggtggccgg
     2221 gttgctgcgg ctgccgtggc tggacatcgc caccgccggg gtgatcggcc tgttgatcgg
     2281 gctggtcgac atcgccgcgc agcagcgccc gcgcctgcag gaagccgcgg acgcgatttc
     2341 cgggctggtt gccggcacgg tggcgatcct ggtgtcgaac ctggtcgccc cgctcaacct
     2401 caacacggtg atcatcgcct cgctgatcgt gttgctgccg ggcatgtcgc tgaccaacgc
     2461 ggtcaacgaa ctcaccagcc ggcacctggt gtccggcacc gcgcgcttcg ccggggcgct
     2521 gaccaccatc ctcaagctca ccatcggcac cgcgatcgcg ctcaccgtcg ccaacctggc
     2581 ggggctgcag ccggaagtgc gcgcgctgcg cccgcagccg gacgtggtca tgtgggcggc
     2641 actgctggtc ggcgcgtatg cgttcgcggt gctgttccgc gcgcatcgcc gcgactaccc
     2701 gctggtgatg ctggcggcga tctccggcta cctgatttcg cggcatggcg gcgaggcctt
     2761 cggcccgcag gccggggtgt tcctcgccgc gctggtcacc accgccgccg gcaatgccta
     2821 tgcgcgatgg atgaaccggc cgggcgcgct gatccggctg cccgggatca tcatgctggt
     2881 gcccggcagc gtggccctgc gcggggtgat ctcgctggtg caggcgcagg acctcggcgc
     2941 cgggcaggag gccgcgatgg cggcgctgaa cacattgatg gcgctgctcg cgggcctgtt
     3001 gttcggcaac ctgctggtgt cggcgcggcg aaacctctag cggccaggcg cggcagcggg
     3061 cccggccagg cggtcgttcc cgaaacaaga acaccggcga atgccggtgt cttcgcgata
     3121 ttcccggtcg atttacttga tcaggaattc ttccagcttg cggcccttgg cggtttccgc
     3181 cgccagccac ttcggctgct tgccgcggcc ggcccaggtg tccttggcat tggccgggtt
     3241 gcggtacttg ggggcgacct tgcccagggt gcggcccttg gtcgacttgc gtgccggctt
     3301 cggggccgcg gccgctgcgg gcctggcggc gccggccgcg ccgtacagct cggcgaaggt
     3361 ccagccgctg gccttgagca gcttgctgac cgccgccttc acctgcgcgg ccggcttgcg
     3421 cttggccagt actttcttgc gctggttggc gcgggaaatc agttccgcca gttccttgct
     3481 gctcaggcct tcgatgttga cgctcatgcg cttgtttcct cgttcgtatc ggttcaaccc
     3541 gatcgggtta atccgatact aaatgccggg gataacccgc gcaaaccgcg gccgggtttc
     3601 ccgttcagcc agcgagcctg tgcagcaggg attcgcggtc cagttgttcg gcctcggcgg
     3661 cgttgcgcgc acggtattcg aaggtgccgg cttccagccc gcgcccggac accaccaccc
     3721 ggtgcgggat gccgatcagc tcgatatccg cgaacatcga acctgcgcgc agcccgcggt
     3781 cgtcgagcac ggcatcgagg ccgcgcgcgt tcaactccgc gtaaagggcg tccgccgcgg
     3841 cagccacgtt cgggtcgttc ttcgggttga tcacgcacac cgcgacctgc cacggcgcca
     3901 tcgccgccgg ccagaggatg ccgttgtcgt cgtggttctg ttcgattgcc gcggcgacga
     3961 tgcgcgacac gccgatgccg tagcagccca tcgccggatg caccgccttg ccgttggtgt
     4021 ccagcacggt ggcgtccatc gcctcggaat acttgcggcc cagcgcgaac acgtggccga
     4081 cctcgatccc gcgggccagg ccgagggtgc cgttgccgtc cggcgaggca tcgccggcga
     4141 ccacgttgcg gatgtcggcg atttccggct ccggcaggtc gcggccccag ttgaccccgg
     4201 cgatgtggaa gcccggcctg ttggcgccga ccacgaagtc cgccagcgcc gccacgctgc
     4261 ggtcggcgat cacgcggatc ggcttgcgcg cgtggagcgg gcccaggaag cccggctcgc
     4321 tgcccaggga ctcggcgatc tccgcttcgc tcgccagccg gtactcggcc aggcccggca
     4381 gcttggccag cttgatctcg ttgaccatgt ggtcgccgcg caccagcgcc agcaccaact
     4441 ggtccttgcc gtccgcatcg gtgccgacca gcgccaccga cttggcggtg cgcgccagcg
     4501 ggatgcccat cagcgcggcc acgtcctcgc aggttttctg ggtgggcgtg tcgatctcgc
     4561 gcagggcctc ggacggcgcc ggacgttcgc ccggcgaaac cgcctcggcc agttccacgt
     4621 tcgccgcgta gtcggagccg tcggaaaacg cgatggcgtc ttcgccggaa tcggccagca
     4681 cgtggaattc gtgcgaggcg ctgccgccga tcgcgccggt atcggcgaac accgcgcgga
     4741 acttcaggcc cagccgcgcg aagatgcgcg agtaggtgtc gtacatgttc cggtattcgg
     4801 cggccagcga gtcttcgtcg atgtggaagg aatacgcgtc cttcatgatg aattcgcgcg
     4861 cgcgcatcac cccgaagcgc gggcggatct cgtcgcggaa cttggtgctg acctggtaga
     4921 agttcaccgg cagctgcttg tagctggcca cttcctggcg gaagaaatcg gtgacggctt
     4981 cctcggcggt gggggtaaag cagtagtcct ggtccttgcg gtcgcggatc ttcagcagct
     5041 gcgggccgaa ctttgcccag cggccggttt cgtcccacag ttccttcggc tggatcgagg
     5101 gcatcgccat ctcgatcgcg ccggcgcggt tcatttcctc gcggacgatg ccct
//
LOCUS       DAORUK010000147         5131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_19871_length_5131_cov_19.565603, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000147 DAORUK010000000
VERSION     DAORUK010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19871_length_5131_cov_19.565603"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..165
                     /locus_tag="PK818_08955"
     CDS             10..165
                     /locus_tag="PK818_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HQY82895.1"
                     /translation="MARGETPKQAAIDMGISDKTVYLHRASLRAKLGVNNDLALHKLA
                     LERGLLG"
     gene            complement(162..1400)
                     /locus_tag="PK818_08960"
     CDS             complement(162..1400)
                     /locus_tag="PK818_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HQY82896.1"
                     /translation="MSATARVLTGLVAGIVIGMLLAWTDPALAATVAGIVEPFGKLWI
                     NALQMTVVPLVLSLVIVGVNHASDAAHSGRVARRAIIIFLVLLAGAATMTAVLAPMAL
                     SLLQPDPAVAAALRGHEAELPQTTGGWANALTAIVPSNAVAAAAAGAMLPLVTFALFF
                     GFALTRIEEARRTQVVDFFKSISDAMIVIVRWVLWVAPVGIFALVLALTARAGLGMLQ
                     ALGIYILLECALYLLAIVLLIAIAATAGRQRLGHFLAGIAPVQAVAASTQSSLATLPA
                     MLESSDRRLGFPQSVSGLVLPMAVSLFRITSPIQYLASASFIAWAMGIDLTTAQLATG
                     VALSVLVSMGSVGLPGQAIFIATNLPVTQAMGLPIAPLPVLMAVDAIPDVLATVGNVT
                     ADMTANALVASRSGDPKAAG"
     gene            complement(1397..2185)
                     /locus_tag="PK818_08965"
     CDS             complement(1397..2185)
                     /locus_tag="PK818_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82897.1"
                     /translation="MTIRRTAPLLLSLVLATACAHRQPAGAEAARWAGAGQLVLVTTA
                     DWDATTGTLRYYQREGTRWRQVGGPFDITVGRTGTAWGIGLHAARNDGPVKREGDGKA
                     PAGVFAIGTAFGYAPSATTGLAYKGMGSNDWCIDVPESSYYNRIIDRSLVKAPHLDKS
                     SEPMRRDIHADGDQRYREGFVIEHNAEGAVANGGSCIFAHLWKAPGEATAGCTAMAPG
                     SMAPLLAWLDARRQPVFVELPLAQYAQLRTAWNLPAIADAEPAR"
     gene            complement(2239..3087)
                     /locus_tag="PK818_08970"
     CDS             complement(2239..3087)
                     /locus_tag="PK818_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HQY82898.1"
                     /translation="MTALVRIRSERDRMSAIERRIADFVLENAHLLRDYSSQQLADAL
                     GISQSSVVKFCQKQGFRGYPDLKLSINEAVVRADSGTADALAHAHAGELPRPAAGLWQ
                     RKSEAEQATRLINPPEALDAAATAIGGARAVFIIGLGEDDIHARAFALRLSLLGILTV
                     HNLDPAHMTASLSAAVPGDVLLVFSEHGMQPALCQLSRHFRAHQHGKVVTITRHNSNA
                     LRAHADIPLLVSAHDERPHVEPLLYHSALQHLLDRVYIRLCEADAGRRARLQDNLVRI
                     QPLLES"
     gene            complement(3098..4204)
                     /locus_tag="PK818_08975"
     CDS             complement(3098..4204)
                     /locus_tag="PK818_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HQY82899.1"
                     /translation="MKITEIRFGMLRVPLKTPFKTALRTVEQVEDVVVMVHTDSGHVG
                     YGSAPATAVITGDTHGSIVEAVRHYIAPRLLGEEIANLNRVTHLVQGALEKNTSAKAA
                     VEIAVHDLWGQLYGAPLYQLLGGGEPAITTDITISVDYIDKMVADSLAAVERGFESLK
                     IKVGKDIGVDIERTRAIHAAVEGRALLRLDANQGWTPKQAVFALQTLEDAGVKLELVE
                     QPVKAHDLAGLRYVTERVHTPVMADESVFGPREVIELIRMRAADIVNIKLMKTGGISN
                     AVKIADICGLYGVDCMIGCMLESSIGVAAAVHLAVAKSDVISKVDLDGPSLCRHDPVA
                     GGVIFNESEITVTDAPGLGIRAIEGLVPIADRAA"
     gene            complement(4215..>5131)
                     /locus_tag="PK818_08980"
     CDS             complement(4215..>5131)
                     /locus_tag="PK818_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004139140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQY82900.1"
                     /translation="QGETDIDRFQESALFPGDAVAIAHASRDGQWLFVVSARYAAWVE
                     AKAIAEGSRDAVLGHGQRTPFRVITGAKPRTVHTREEPRVSELQLDMGTRIPLAGVAP
                     DQPVNGQHPYAAWILELPVRGADGRLGFAPALLQRNSDSAADVLPLTRANLIRQAFRF
                     LGERYGWGHAYNGRDCSGFVSEVYRSMGVQLPRNTSDQAVSPVFARTRFEPGDSREKR
                     MAAVDALDVGDLIYIPGHVMMFLGRIDGMPYVIHDTNGGTILGADGEPRSLHLNGVSV
                     TPLLPLRFGAGHDYVDRITNIVRVAAPQ"
BASE COUNT          771 a         1735 c         1833 g          792 t
ORIGIN      
        1 ttcctgcgcc tggcgcgcgg ggaaaccccc aagcaggcgg ccatcgacat ggggatcagc
       61 gacaagaccg tgtacctgca ccgcgcctcg ctgcgagcca agctcggcgt caacaacgac
      121 ctggccttgc acaagctggc gctggaacgg ggcttgctgg gctagcctgc ggctttggga
      181 tccccgctgc gcgacgccac cagagcgttg gcggtcatgt cggcggtgac gttgccgacg
      241 gtggccagca cgtccgggat cgcgtccacc gccatcagca ccggcagcgg ggcgatcggc
      301 aggcccatcg cctgggtcac cggcaggttg gtggcgatga agatcgcctg cccgggcagc
      361 ccgaccgaac ccatgctgac cagcaccgac agggcgacgc cagtggccag ttgcgcggtg
      421 gtcaggtcga tgcccatcgc ccaggcgatg aagctggcgc tggcgaggta ctggatcggc
      481 gaggtgatgc ggaacagcga gaccgccatc ggcagcacca gccccgacac cgattgcggg
      541 aagccgagcc ggcggtccga gctctccagc atcgccggca aggtggccag cgaggactgc
      601 gtgctggcgg cgaccgcctg caccggcgcg atgccggcca ggaaatgccc cagccgctgg
      661 cgcccggcgg tggcggcgat ggcgatcagc agcacgatcg ccagcaggta cagcgcgcac
      721 tccagcagga tgtagatgcc cagcgcctgc agcatgccca gcccggcgcg cgcggtgagt
      781 gccagcacca gcgcgaagat ccccaccggc gccacccaca gcacccagcg cacgatcacg
      841 atcatcgcgt cgctgatcga cttgaagaag tcgaccacct gggtgcgcct ggcttcctcg
      901 atgcgggtca gggcgaagcc gaagaacagc gcgaaggtca ccagcggcag catcgcgccg
      961 gcggccgcgg cggccacggc gttgctggga acgatcgcgg tcagtgcgtt ggcccagccg
     1021 ccggtggtct gcggcaattc ggcttcatgg ccgcgcaacg cggcggcgac cgccggatcc
     1081 ggctgcagca gcgacagcgc catcggcgcc agcaccgcgg tcatggtcgc ggcgccggcc
     1141 agcagcacca ggaagatgat gatcgcgcgg cgggcgacgc gcccggagtg ggcggcgtcg
     1201 gatgcatggt tgacgccgac gatcaccagc gacagcacca gcggcaccac ggtcatctgc
     1261 agcgcattga tccacagctt gccgaacggt tcgacgatgc cggcgacggt ggcggccagc
     1321 gctggatcgg tccatgccag cagcatgccg atcacgatgc cggcgaccag gccggtgagg
     1381 acgcgtgcgg tggcgctcat cgcgccggct ccgcgtccgc gatcgccggc aggttccatg
     1441 ccgtgcgcaa ttgcgcgtac tgcgccagcg gcaattccac gaacaccggt tgcctgcggg
     1501 cgtccagcca ggccagcaac ggcgccatgc tgccgggcgc catcgcggtg caaccggcgg
     1561 tggcctcgcc cggtgccttc cacaggtggg cgaagatgca gctgccgccg ttcgccaccg
     1621 cgccctcggc gttgtgttcg atcacgaagc cctcgcgata gcgctggtcg ccatcggcgt
     1681 ggatgtcgcg gcgcatcggc tcgctggatt tgtcgaggtg cggcgccttg accaggctgc
     1741 ggtcgatgat gcggttgtaa taggacgatt cgggcacgtc gatgcaccag tcgttgctgc
     1801 ccatgccctt gtaggcaagg ccggtggttg cgctgggtgc gtagccgaac gcggtgccga
     1861 tcgcgaacac gccggcgggc gccttgccgt cgccctcgcg cttgaccggg ccatcgttgc
     1921 gcgccgcatg caggccgatg ccccaggcgg tgccggtgcg gcccacggtg atgtcgaacg
     1981 gcccaccgac ctggcgccag cgggtgcctt cgcgctggta gtagcgcagg gtgccggtcg
     2041 tggcatccca gtcggcggtg gtgaccagca ccagttgccc ggcgcctgcc cagcgcgcgg
     2101 cttcagcccc cgcgggttgg cgatgcgcgc aggcggtggc caggaccagc gacagcagca
     2161 ggggggcggt ccggcgaatc gtcatgcggg cgttccgggt ggggaaggtg gatcgtagtg
     2221 gaaagccggc aagaccggtc acgactcaag cagcggctgg atgcggacca ggttgtcctg
     2281 caggcgcgcg cggcggccgg cgtcggcttc gcacaggcgg atgtagacgc ggtcgagcag
     2341 gtgctgcagc gcggagtggt agagcagggg ttcgacgtgc gggcgttcgt cgtgggcgga
     2401 gaccagcagg gggatgtccg cgtgcgcgcg cagcgcattc gagttgtggc gggtgatggt
     2461 gaccaccttg ccgtgctggt gggcgcggaa atgccggctc agctggcaca gcgccggttg
     2521 catgccatgc tcggagaaca ccagcagcac gtcgccgggc actgccgcgg acaggctggc
     2581 ggtcatgtgc gccgggtcga ggttgtgcac ggtcaggata cccagcaacg acagccgcag
     2641 cgcgaatgcg cgcgcatgga tgtcgtcctc gcccaggccg atgatgaaca ccgcgcgcgc
     2701 gccgccgatc gccgtggccg ccgcgtccag cgcctcgggc gggttgatca ggcgggtggc
     2761 ttgctccgct tccgacttgc gctgccacag gcccgccgcg ggccgcggga gctcgcccgc
     2821 atgcgcgtgg gccagcgcgt ctgcggtgcc gctgtccgcg cgcaccaccg cctcgttgat
     2881 cgacagcttg aggtcgggat agccgcggaa gccctgcttc tggcagaact tgaccacgct
     2941 ggactggctg atcccgagcg cgtccgccag ctgctgcgag gagtagtcgc gcagcaggtg
     3001 cgcgttctcc agcacgaagt cggcgatccg gcgctcgatc gccgacatcc ggtcgcgctc
     3061 ggaacggatc ctgaccagcg ccgtcatcgt gcgtgcctca ggcggcccgg tccgcgatcg
     3121 gcaccaggcc ctcgatcgcg cggatgccca gccccggcgc atcggtcacg gtgatctccg
     3181 actcgttgaa gatcacgccc ccggccaccg ggtcgtggcg gcacagcgaa ggaccatcga
     3241 ggtcgacctt gctgatcacg tcggacttcg ccaccgccag gtgcaccgcg gcggccacgc
     3301 cgatgctgga ctcgagcatg cagccgatca tgcagtccac gccgtacagg ccgcagatgt
     3361 cggcgatctt caccgcgttg gagatgccgc cggtcttcat cagcttgatg ttgacgatgt
     3421 cggcggcgcg catgcggatc agttcgatca cctcgcgcgg gccgaacacg ctctcgtcgg
     3481 ccatcaccgg cgtgtgcacg cgttcggtca cgtagcgcag gccggccagg tcgtgcgcct
     3541 tcaccggctg ttcgaccagt tccagcttca ccccggcgtc ctccagcgtc tgcaacgcaa
     3601 acacggcctg tttcggggtc cagccctggt tggcgtccag ccgcagcagg gcgcggccct
     3661 cgaccgcggc atggatcgcc cgggtgcgct cgatatcgac cccgatgtcc ttgccgacct
     3721 tgatctttag cgactcgaag ccgcgttcga ccgcggccag cgagtccgcc accatcttgt
     3781 cgatgtagtc cacgctgatg gtgatgtcgg tggtgatcgc cggttcgccg ccacccagca
     3841 actggtagag cggtgcgccg tacagctggc cccacaggtc gtgcaccgcg atctcgaccg
     3901 cggccttggc gctggtgttc ttttccagcg cgccctgcac caggtgggtg acccggttga
     3961 ggttggcgat ctcctcgccg agcaggcgcg gggcgatgta atggcgcacc gcctcaacga
     4021 tggagccgtg ggtatcgccg gtgatcaccg cggtcgccgg ggcgctgccg tagccgacgt
     4081 ggccgctgtc ggtatgcacc atcaccacca cgtcctcgac ctgctcgacg gtgcgcagcg
     4141 cggtcttgaa cggcgtcttc agcggcacgc gcagcatgcc gaagcggatt tcggtgatct
     4201 tcatgggggt gtcctcattg cggcgctgcc accctgacga tgttggtgat gcggtccacg
     4261 taatcgtgcc cggcgccgaa gcgcagcggc agcagcggcg tcaccgatac gccgttgagg
     4321 tgcagcgagc ggggttcgcc gtcggcaccg aggatggtgc cgccgttggt gtcgtggatc
     4381 acgtagggca tgccgtcgat gcgaccgagg aacatcatca cgtgcccggg gatgtagatc
     4441 aggtcgccca cgtccagcgc atcgaccgcg gccatgcgct tttcgcgcga atcgccgggt
     4501 tcgaagcggg tgcgcgcgaa caccgggctg actgcctggt cgctggtgtt gcgcggcagc
     4561 tgcacgccca tgctgcggta gacctcggac acgaagccgc tgcagtcgcg gccgttgtag
     4621 gcatggcccc agccatagcg ctcgccaagg aacctgaatg cctggcggat gagattcgcg
     4681 cgggtcagcg gcaggacgtc ggcggcgctg tcggaattgc gttgcagcag cgcaggcgcg
     4741 aagccgagtc gcccatcggc gccgcgcacg ggcaactcga ggatccacgc ggcatatgga
     4801 tgctggccgt tgaccggctg gtccggggcg acaccggcca gcgggatgcg cgtgcccatg
     4861 tccagttgca gttcggacac gcgcggttcc tcgcgggtgt ggacggtgcg cggcttggcg
     4921 ccggtgatca cgcggaacgg cgtgcgctgg ccatggccca gcaccgcatc gcggctgcct
     4981 tccgcgatcg ccttcgcttc cacccaggcg gcatagcggg cgctgaccac gaacagccat
     5041 tgcccgtccc ggctggcatg cgcgatcgcc accgcgtcgc ccgggaacag cgcgctttcc
     5101 tggaagcggt cgatgtcggt ttcgccctgg c
//
LOCUS       DAORUK010000148         5042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_20430_length_5042_cov_15.102867, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000148 DAORUK010000000
VERSION     DAORUK010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20430_length_5042_cov_15.102867"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PK818_08985"
     CDS             complement(<1..809)
                     /locus_tag="PK818_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82901.1"
                     /translation="MKLQLNALCLALLGGAASLLAPVEAQAGNVGHFSACTGGSAATL
                     ITLAGHTPVSVATLDNASLAPLDGLVISQCGAPYAGSSAVDAAVANGLRVLIDTQSVP
                     PASDLPGDPALTYDGAGMCASGLSITPGAAITSGPGGTLTDSSLDQTSICSSMGSALV
                     SSLPAGGRSFVEVAGNPGSSIAFAYPSGNGWVAVSFSQWMFTLPGGGYESDSIAPGAY
                     TYYANTIAWMMGEILPVTTCASEGYTGTKLEWCKNICERGYAGSTLAMWIRR"
     gene            1022..1978
                     /gene="deoC"
                     /locus_tag="PK818_08990"
     CDS             1022..1978
                     /gene="deoC"
                     /locus_tag="PK818_08990"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HQY82902.1"
                     /translation="MTVPVRDRDGSLRGIPRVAVDAVGAGERAGFFAKRSVKAASKLD
                     LLYLAIRMMDLTTLEGADTPARVRQMCRKAMQPAAPALTEALQKVEGFRPIPPVAAVC
                     VYPLLVPHAVKALEGSGVHVASVATAFPSGQATLKTRLVDVHEAVEGGADEIDMVINR
                     GAFLAGEYQQVFDEIAAVREACGAAHLKVILETGELGTLDNVRLASDLAIAAGAHFIK
                     TSTGKIVPAATPPVTLVMLQAIADHHARTGEKIGMKPAGGIRNAKQSIQYLCMLQETL
                     GPAWMTPELYRFGASALLNDVLRQVYRQYTGRYYYDKGYSLD"
     gene            1971..3461
                     /locus_tag="PK818_08995"
     CDS             1971..3461
                     /locus_tag="PK818_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQY82903.1"
                     /translation="MTKTTAKGGRAAAAPGTVIDYAPSIESDKIVTIAPRHELYIGGR
                     WTAPAEGRYFASVNPATLKPLSQIAEAGSADVDTAVRAAKRAYDRTWSKLSGRERGRY
                     LFRIARLIQERARELAVLETLDNGKPIRETRDFDIPLVAAHFFYYAGWADKLGYALPG
                     ATLAPLGVAGQIIPWNFPLLMAAWKIAPALACGNTVVLKPAEQTSLSVLKLVEIFEAA
                     GLPPGVVNIVTGAGATGAALVAHPLVRKVAFTGSTEVGKAIARSVAGSGKKLTLELGG
                     KSAHIVFADAAIDQAVEGVVNGIFFNQGHVCCAGSRLLVEESVAERVLDKLKARMRTL
                     RVGNPLDKNTDIGAINSTAEFDKIRGLVETGKREGATYFEPDNVVLPAKGNFLRPCLF
                     TDVASAHTICRVEVFGPVLAVSTFRTPAEAIAKANDTPYGLAAGIWSEKGSKVHAVAA
                     QLRAGVVWANTYNKFDPAAPFGGYGESGYGREGGRHGLLPYLEVVA"
     gene            3458..4321
                     /locus_tag="PK818_09000"
     CDS             3458..4321
                     /locus_tag="PK818_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQY82904.1"
                     /translation="MSRTESARLPVPKTHKLYIGGEFPRSESGRSYVLAKPGAKHAYA
                     QLCLASRKDLRAAVDAAKAAQPGWAGRSAYNRGQILYRMAEMIEGRGESWRALLRDGL
                     GLTAAQATRAFEGVIDALVYFAGATDKYPQVIGAVNPVAGPHHSFTTPEPTGVIGLIF
                     PDEPDPAAIAARIAAAIAAGNSVIALLPGRAGGLVAEFGEAFATADLPGGVVNLLSGD
                     AAELTPQFASHMEIQGLAFLRDEPQQRTELERAAADNMKRCVASHGPLLALERVLDFV
                     EYKTVWHPIGS"
     gene            complement(4340..5041)
                     /locus_tag="PK818_09005"
     CDS             complement(4340..5041)
                     /locus_tag="PK818_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQY82905.1"
                     /translation="MPLHFTQRGVNRAATFLDDEDFAQYRQKLGEVLAEHDIALHAYV
                     LMTNHVHLLLTPPLAGRLSAAMCQLGRRYVPAFNRKHGRTGTLWEGRFKTCLVDSDRY
                     ALTVQRYIELNPVRAGMTASAGAYRWSSASASLGGAADALLSFHPVYLALGEDARARA
                     SAYREWLALGIGADEARSIREHLRQERALGQPHFQEMAEKALNRPVAIRGRGRPRTSR
                     VPGVPAGLLPDSNAN"
BASE COUNT          831 a         1773 c         1710 g          728 t
ORIGIN      
        1 cggcggatcc acatcgccag ggtgctgccg gcatagccgc gttcgcagat gttcttgcac
       61 cattccagct tggtaccggt gtagccctcg ctggcgcagg tggtcaccgg gaggatttcg
      121 cccatcatcc aggcgatggt attggcgtag taggtataag cgccgggagc gatcgaatcg
      181 ctctcgtagc caccacccgg caaggtgaac atccattgcg agaaggaaac cgccacccag
      241 ccgttgccag agggataggc gaacgcgatg gacgagccgg gattgcctgc gacctccacg
      301 aacgatcgcc cgcccgcagg caagctggac accagcgccg aacccatcga agagcagatc
      361 gacgtctggt ccaggctgct gtcggtcagg gtgccgccgg ggccgctggt gatcgctgcg
      421 cccggggtga tcgaaaggcc gctggcgcac atgccggcgc catcgtaggt cagcgcaggg
      481 tcgcccggca ggtcggatgc tggggggacg ctctgggtgt cgatcaggac gcgcaggcca
      541 ttggcaactg ctgcgtcgac cgcgctggaa cctgcgtacg gtgcaccgca ctgcgagatc
      601 accaggccat ccagaggtgc cagcgacgcg ttgtccagcg tggcgacgct taccggcgta
      661 tgtccggcca gcgtgatcag ggttgccgca ctgccaccgg tgcaggccga gaaatggccc
      721 acgttgccgg cttgcgcctc gacaggcgcc agcaggcttg cagcaccacc cagcaatgca
      781 aggcagagcg cattcaactg caacttcatg actcttcccc tctttccgcc cgacccccgg
      841 gcgcatgcgt ccagtgtacg actaacggcc aaaaaggggc ggccaaaaag gggccagagt
      901 caattaccgt ttgaatcgcg ccgaaccgcc gcgcccgctg gccaccgggc gccgctgccg
      961 tgacggcaac cgggccagca cgtatccttg gcccctgctt tcccgtgaga gtccggtgtc
     1021 catgactgtt cccgtccgcg accgcgacgg ttcgctgcgc ggcatcccga gggtggcggt
     1081 cgatgccgtg ggcgcaggtg agcgcgccgg cttcttcgcc aagcgcagcg tcaaggcggc
     1141 gagcaagctc gacctgctct acctcgccat ccgcatgatg gacctgacca cgctggaggg
     1201 cgccgacacc ccggcgcggg tgcggcagat gtgccgcaag gcgatgcagc cggcggcgcc
     1261 ggcgctgacc gaggcgctgc agaaggtcga gggcttccgg ccgatcccgc cggtggccgc
     1321 ggtctgcgtg tatccgctgc tggtgccgca tgcggtcaag gcgctggagg gctcgggcgt
     1381 gcacgtggcg tccgtggcca ccgcgttccc gtccgggcag gccacgctga agacgcggct
     1441 ggtcgacgtg cacgaggcgg tcgagggtgg cgccgacgag atcgacatgg tgatcaaccg
     1501 cggcgcgttc ctggccggcg agtaccagca ggtgttcgac gagatcgccg cggtgcgcga
     1561 ggcctgcggc gcggcgcacc tcaaggtgat cctggaaacc ggcgaactcg gcacgctcga
     1621 caacgtccgg cttgcgtcgg acctcgccat cgccgccggc gcgcatttca tcaagacctc
     1681 gaccggcaag atcgtgccgg ccgcaacgcc gccggtgacg ctggtgatgc tgcaggcgat
     1741 cgccgaccac cacgcccgca ccggcgagaa gatcggcatg aagccggccg gcggcatccg
     1801 caacgccaag cagtcgatcc agtacctgtg catgctccag gagaccctgg gtccggcgtg
     1861 gatgacgccg gagctgtacc gcttcggcgc cagcgccctg ctcaacgacg tgctgcggca
     1921 ggtctaccgc cagtacaccg gccgctacta ctacgacaag ggctactcgc ttgactaaga
     1981 ccaccgccaa gggcggccgc gccgccgcag cgcccgggac cgtcatcgac tacgccccga
     2041 gcatcgaatc cgacaagatc gtgaccatcg cgccgcgcca tgagctgtac atcggcgggc
     2101 gctggaccgc gcctgccgag ggccgctact tcgccagcgt caacccggcc acgctgaagc
     2161 cgctgtcgca gattgccgag gccggcagcg ccgacgtcga taccgcggtg cgcgccgcca
     2221 agcgcgccta cgaccggacc tggagcaagc tgtccgggcg cgagcgcggc cgctacctgt
     2281 tccgcatcgc gcggctgatc caggagcgtg cgcgcgaact ggccgtgctg gagacgctgg
     2341 acaacggcaa gccgatccgc gagacccgcg acttcgacat cccgctggtc gccgcgcact
     2401 tcttctacta cgccggctgg gccgacaagc tcggctatgc gctgcccggg gccacgctgg
     2461 cgccgctggg cgttgccggc cagatcatcc cgtggaactt cccgctgctg atggcggcgt
     2521 ggaagatcgc gccggcgctg gcctgcggca acaccgtggt gctcaagccc gccgagcaga
     2581 cctcgctgtc ggtgctcaag ctggtggaga tcttcgaggc cgccggcctg ccgccggggg
     2641 tggtcaacat cgtcaccggg gccggcgcca ctggcgctgc actggtcgcg catccgctgg
     2701 tgcgcaaggt cgccttcacc ggcagcaccg aggtcggcaa ggcgatcgcc cgcagcgtcg
     2761 ccggcagtgg caagaagctg accctggaac tgggcggcaa gtcggcgcac atcgtgttcg
     2821 ccgatgccgc catcgaccag gcggtggagg gcgtggtcaa cggcatcttc ttcaaccagg
     2881 gccacgtctg ctgcgccggt tcgcggctgc tggtcgagga atcggtggcc gaacgcgtgc
     2941 tcgacaagct caaggcgcgc atgcggaccc tgcgcgtcgg caacccgctg gacaagaaca
     3001 ccgacatcgg cgcgatcaac tccaccgccg agttcgacaa gatccgcggc ctggtcgaaa
     3061 ccggcaagcg cgagggcgcg acgtatttcg agccggacaa cgtcgtgctg ccggccaagg
     3121 gcaacttcct gcggccgtgc ctgttcaccg acgtcgcctc cgcgcacacc atctgccggg
     3181 tcgaggtgtt cggcccggtg ctggcggtgt cgaccttccg caccccggcc gaggcgatcg
     3241 ccaaggccaa cgacacgccg tatggcctgg ctgccggcat ctggagcgag aagggctcga
     3301 aggtgcatgc ggtcgccgcg caactgcgcg ccggcgtggt ctgggccaac acctacaaca
     3361 agttcgatcc ggcggcaccg ttcggcggct acggcgaaag cggctatggc cgcgaaggcg
     3421 ggcgccatgg gctgctgccc tacctggagg tggtggcatg agccgcaccg agtccgcgcg
     3481 cctgccggtg ccgaagacgc acaagctgta catcggcggc gaattcccgc gcagcgaatc
     3541 cgggcgcagc tacgtgctcg ccaaaccggg tgcgaagcac gcctacgcgc agctgtgcct
     3601 ggcctcgcgc aaggacctgc gcgccgcggt ggatgcagca aaggccgcgc agcccggctg
     3661 ggcggggcgt tcggcgtaca accgcgggca gatcctgtac cgcatggccg agatgatcga
     3721 aggccgcggc gaatcctggc gtgcgctgtt gcgtgatggc cttggcctca ccgcggcgca
     3781 ggcgacgcgc gcgttcgagg gcgtgatcga cgcgctggtc tacttcgccg gcgccaccga
     3841 caagtacccg caggtcatcg gcgcggtgaa cccggtggcc gggccgcacc acagcttcac
     3901 cacccccgaa cccaccggcg tcatcggcct catctttccc gacgaaccgg acccggccgc
     3961 gatcgccgcg cgcatcgccg ccgcgatcgc cgccggcaac agcgtgatcg cgctgctgcc
     4021 gggccgcgcc ggtggcctgg tcgccgaatt cggcgaagcc ttcgccaccg ccgacctgcc
     4081 cggcggcgtg gtcaacctgc tctccggcga tgccgccgaa ctcaccccgc agttcgccag
     4141 ccacatggaa atccagggcc tggccttcct gcgcgacgaa ccgcagcaac gcaccgaact
     4201 cgagcgcgcc gccgccgaca acatgaagcg ctgcgtcgcc agccacggcc cgctgctggc
     4261 gctggagcgg gtgctcgact tcgtcgaata caagaccgtc tggcacccca tcggcagcta
     4321 aaacaaaaag gggccagagt caattagcgt ttgaatccgg gagcagtccc gcaggcacgc
     4381 ctggaacgcg tgatgtccgc ggccgccccc gcccgcggat ggcgacgggt cggttcaggg
     4441 ctttctcggc catttcctgg aagtgcggct ggccgagtgc gcgctcttgc cgcaggtgct
     4501 cgcggatcga ccgcgcctcg tccgcgccaa tgcccagcgc cagccattcc cgataggccg
     4561 aagcccgggc acgcgcgtcc tcgcccagcg ccaggtacac cggatggaac gacagcagcg
     4621 cgtccgccgc accgccaagg ctggcactcg cactggacca gcggtacgcc ccggctgatg
     4681 ccgtcatccc ggcgcgcacg ggattcagct cgatgtagcg ctgcacggtc agcgcatagc
     4741 ggtcggagtc gaccaggcag gtcttgaacc gcccctccca caaggtgccg gtccgcccat
     4801 gcttgcgatt gaaggccggc acgtagcgcc gccccaactg gcacatggcc gcggacaagc
     4861 ggcccgccag cggcggcgtc agcaacaggt ggacgtggtt cgtcatcagc acataggcgt
     4921 gcagggcgat gtcgtgctcg gccagcacct cgcccagttt ctggcggtac tgcgcgaagt
     4981 cctcgtcatc caggaaggtc gccgcccggt tcacgccacg ctgggtgaag tgaagcggga
     5041 tg
//
LOCUS       DAORUK010000149         4982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_20800_length_4982_cov_16.328800, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000149 DAORUK010000000
VERSION     DAORUK010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20800_length_4982_cov_16.328800"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PK818_09010"
     CDS             <1..302
                     /locus_tag="PK818_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005913764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY82906.1"
                     /translation="HQVEEIEHILTDVMFIRDGKIVLNAPMDELGNRYAEVLVNADKL
                     DAARGLKPIDERALPFGKTVMLFDGANPGQLALLGETRTPGLADLFVATMKGTYA"
     gene            320..1366
                     /locus_tag="PK818_09015"
     CDS             320..1366
                     /locus_tag="PK818_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82907.1"
                     /translation="MALDADRPRAPRAAPHATHKLKLLLRREFWEHKGGFLWAPVWAG
                     GISLVLTLMALIFGEVAARRAAASGKLQIDGDVMINGLDLGMITSKMDAEDLRKLAGG
                     IDLSMLSSSMWPLIVLGFVVFFYCLGSLYDERKDRSVLFWKSLPVSDRDTVLSKAASA
                     LLVAPTLAIGAAIATMFAFLLLLSVFVLLHNGNPIQLLWGAGNPLANAGLMLAAIPVY
                     ALWALPTVGWLLLCSAWSRSKPFLWAIVIPLFAGIFITWFDVMNLFDLESGWFWKNVV
                     ARLLGGVFPGTWIDVANTDGLRFKTPGDMFGHLDALKAMYSVLATTQLWIGAAAGIAM
                     ITGAIRLRRWRDEG"
     gene            1405..2073
                     /locus_tag="PK818_09020"
     CDS             1405..2073
                     /locus_tag="PK818_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82908.1"
                     /translation="MKRILAVALLASAPLSGVQAHGNHGTQVGIGAEIRHELADARKE
                     VRIDLAKARRELETDNLRLDKGLRFGQDGTRKQARNTALPQAEITPRGDFLIDGKPQA
                     IDGGQRRQLLAYRGQVIGIAKSGIDIGQRSAEAALDAVGNGSLFGLVVGAMTGSLERR
                     IERLVRAEVEPGVRGICRQLPALLQAQQRLASSLAQFRPYATLEAGDVDDCEKQVRRD
                     FASL"
     gene            2090..2779
                     /locus_tag="PK818_09025"
     CDS             2090..2779
                     /locus_tag="PK818_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82909.1"
                     /translation="MKQIHALALALLAGTTLLACSEPPAPPAPPAPPAPPAGSASPKG
                     FIGREVEKAITEARQELRNGNISISDGFNLNVNGTQVHTTDAGLPKAEITPQGDLLVE
                     GKAVPVSAGQREQLLDYRGRIIGLAEAGMAIGVQGADIAGEAIAGVAGAIFGGKEGEQ
                     AFEQRMQAQGKKIEAEAMKLCTQLPGLLASQQALAASLPAFQPYARMTQEDIDDCGKH
                     KTGNGVSVTSN"
     gene            complement(2817..4226)
                     /locus_tag="PK818_09030"
     CDS             complement(2817..4226)
                     /locus_tag="PK818_09030"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009603394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HQY82910.1"
                     /translation="MARRSGFRIEHDSMGDLQVPAEALWGAQTQRALDNFRVSGRPMP
                     AGFVRALAQVKGAAAAVNGRLGLLDAARAEAIATAARAVADGGHDGQFPLDRYQTGSG
                     TSSNMNANEVVAHLAARASGLAIHPNDHVNLGQSSNDAVPTAIRVMAVHAATSDLLPA
                     LKHLRKSIDKRAKAIGKTVKTGRTHLMDAMPLTFAQEFGAWSAQLASAQARIEDTLER
                     VRRLPIGGTAIGTGINAHPKFGAGMAKALSATTGAKFESAADRFEGLAAQDDLVELSG
                     QLNVLAVALMKIGNDLRWMNSGPLAGLGEIELPALQPGSSIMPGKVNPVIPEALCMVC
                     AQVMGLHHAISIAGQSGNFQLNVMLPLIACDLDESIQLLANGMRALADKAIAGLTIRK
                     DRVAATLARNPILVTALNPVIGYEAAAKIAKRAYAENRPVLEVALEDSGLPEKTLRHL
                     LDPARLTKGGIGSGSASST"
     gene            4307..>4982
                     /locus_tag="PK818_09035"
     CDS             4307..>4982
                     /locus_tag="PK818_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HQY82911.1"
                     /translation="MPDALTALSPLDGRYAGKVDALRPIFSEYGLVRARVKVEVEWLL
                     ALAEAPAIAGLPPFSEAAAARLRALADGFSVADAARVKEIERTTNHDVKAVEYLIKER
                     LQDDAELGPALEFVHFACTSEDINNLSYALMLNQARLSVLLPKLDDLIQKLRAMAHEH
                     AALPMLSRTHGQTASPTTVGKELANVVARLQRQGEVLAGLPMPGKINGAVGNYNAHVA
                     SYPDIDW"
BASE COUNT          783 a         1738 c         1681 g          780 t
ORIGIN      
        1 cccaccaggt cgaggagatc gaacacatcc tcaccgacgt gatgttcatc cgcgacggca
       61 agatcgtgct gaacgcgccg atggacgagt tgggcaaccg ctacgccgag gtgctggtca
      121 atgcggacaa gctggatgcc gcgcgcggcc tgaaacccat cgacgagcgc gccctgccgt
      181 tcggcaagac cgtgatgctg ttcgacggcg ccaaccccgg gcagctggcc ctgctcggcg
      241 aaacccgtac gccgggcctg gccgacctgt tcgtcgccac catgaaggga acctacgcat
      301 gaacacctct gttgacgcga tggcactgga cgccgaccgt ccgcgcgcgc cgcgcgccgc
      361 gccgcacgcc acccacaagc tgaagctgct gctgcgccgc gaattctggg agcacaaggg
      421 cggattcctg tgggccccgg tctgggccgg cggcatttcg ctggtgctga ccctgatggc
      481 actgatcttc ggtgaagtcg cggcccgccg cgccgccgcc agcggcaagc tgcagatcga
      541 tggcgacgtc atgatcaacg gcctcgacct tggcatgatc acctcgaaga tggatgccga
      601 ggacctgcgc aagctggccg gcggcatcga cctcagcatg ctgtcctcgt cgatgtggcc
      661 gctgatcgtg ctcggcttcg tcgtgttctt ctactgtttg ggcagcctgt acgacgagcg
      721 caaggaccgc agcgtgctgt tctggaagtc gctgccggtt tccgaccgcg acaccgtgct
      781 gtcgaaggcc gccagtgccc tgctggtggc gccgaccctg gcgatcggcg cggcgatcgc
      841 gaccatgttc gcgttcctgc tgctgctcag cgtgttcgtg ctgctgcaca acggcaaccc
      901 gatccagttg ctgtggggcg cgggcaatcc gctggcgaac gccggcctga tgctggcggc
      961 gatcccggtg tacgcgctgt gggcgctgcc gaccgtgggc tggctgctgc tgtgctcggc
     1021 gtggtcgcgc agcaagccgt tcctgtgggc gatcgtgatc ccgctgttcg ccggcatctt
     1081 catcacctgg ttcgacgtga tgaacctgtt cgacctggaa agcggctggt tctggaagaa
     1141 cgtggtcgcc cgcctgctgg gcggcgtgtt cccgggcacc tggatcgacg tcgccaacac
     1201 cgacggcctg cgcttcaaga ccccgggcga catgttcggc cacctggatg cgctgaaggc
     1261 gatgtactcg gtgctggcca ccacccagct gtggatcggc gcggcggcgg gcattgcgat
     1321 gatcaccggc gcgatccgcc tgcgccgctg gagggacgag ggctgaggcc ctcgttccac
     1381 ccgaccccaa cacaggaccc tgacatgaag cgaatcctcg ccgtcgccct gctcgccagc
     1441 gccccgttgt ccggtgtgca ggcccacggc aaccatggca cccaggtcgg catcggtgcc
     1501 gagatccgcc acgaactcgc cgacgcgcgc aaggaagtgc gcatcgacct ggccaaggcc
     1561 aggcgcgagc tggagaccga caacctgcgc ctggacaagg gcctgcgctt cggccaggac
     1621 ggcacgcgca agcaggcacg caacacggcg ctgccgcagg ctgaaatcac cccgcgcggc
     1681 gatttcctga tcgacggcaa gccgcaagcc atcgacggcg gccagcgccg tcagctgctg
     1741 gcctaccgcg gccaggtgat cggcatcgcc aagagcggga tcgacatcgg ccagcgcagc
     1801 gccgaagcgg cgctggatgc ggtcggcaat gggtccctgt tcggacttgt cgtgggcgcg
     1861 atgaccggaa gcctggaacg ccgcatcgag cgcctggtca gggcggaagt cgagcccggc
     1921 gtgcgcggca tctgccggca actgccggcg ctgctgcagg cccagcagcg actggcgtcc
     1981 agcctcgcgc agttccggcc ctatgccacc ctcgaagcgg gtgacgtgga cgactgcgaa
     2041 aagcaggtcc gccgcgattt cgcatcactt tgacccggag ccacgcgcaa tgaagcagat
     2101 ccacgccctc gccctcgccc tgcttgccgg caccacgttg ctcgcctgca gcgagccccc
     2161 cgcgccgccg gcgccgcccg ccccaccagc gccgcccgcg ggcagcgcgt ccccgaaggg
     2221 cttcatcggt cgcgaggtgg agaaggcgat caccgaagcc cgccaggagc tgcgcaacgg
     2281 caacatcagc atcagcgacg gcttcaacct gaacgtcaac ggcacgcagg tgcacaccac
     2341 cgacgccggg ctgccgaagg cggagatcac cccgcagggc gacctgctgg tcgagggcaa
     2401 ggcggtgccg gtcagcgccg ggcagcgcga gcaattgctc gactaccgcg gccggatcat
     2461 cggccttgcc gaggccggca tggcgatcgg cgtgcagggt gcggacatcg ccggcgaggc
     2521 gatcgccggc gtggccggcg cgatcttcgg tggcaaggag ggcgaacagg ccttcgagca
     2581 gcggatgcag gcgcagggca agaagatcga ggccgaagcg atgaagcttt gcacgcaatt
     2641 gccggggctg ctggcgagcc agcaggcgct tgcggcctcg ctgcccgcgt tccagccgta
     2701 tgcgcggatg acccaggaag acatcgacga ttgcggcaag cacaagaccg gcaacggcgt
     2761 cagcgtcacc agcaactgat ggttgtcccg gtccggcgtg ctgcgccggg ccgggttcag
     2821 gtactgcttg ccgagccgct gccaatccct cccttcgtca atcgggccgg atcgagcagg
     2881 tggcgcaagg tcttttccgg cagtccgctg tcctccagcg ccacctccag caccgggcgg
     2941 ttttccgcat acgcgcgctt ggcgatcttc gccgccgcct cgtagccaat caccggattc
     3001 agcgcggtga ccaggatcgg gttgcgcgcc agcgtggccg ccacccggtc cttgcggatc
     3061 gtcagtcccg cgatcgcctt gtccgccagt gcgcgcatgc cattcgccag caactggatc
     3121 gactcgtcga ggtcgcaggc gatcagcggc agcatcacgt tgagctggaa gttgccgctc
     3181 tggccggcga tggagatggc gtggtgcagt cccatcacct gcgcgcacac catgcacagc
     3241 gcttccggga tgaccgggtt gaccttgccc ggcatgatcg aggaaccggg ctgcagcgcc
     3301 ggcagttcga tctcgcccag gccggccagc gggccggaat tcatccagcg caggtcgttg
     3361 ccgatcttca tcagcgccac cgccaacacg ttcaactgcc ccgacaattc gaccaggtcg
     3421 tcctgcgcgg ccaggccttc gaacctgtcg gcggcggatt cgaatttcgc accggtggtg
     3481 gccgacagcg ccttcgccat gcctgcgccg aacttcggat gcgcgttgat cccggtgccg
     3541 atcgcggtgc cgccgatcgg caggcggcgc acgcgctcca gggtgtcctc gatgcgtgcc
     3601 tgcgcggacg ccagctgcgc ggaccaggca ccgaattcct gggcaaacgt cagcggcatg
     3661 gcgtccatca ggtgggtgcg gccggtcttg accgtcttgc cgatggcctt ggcgcgcttg
     3721 tcgatggact tgcgcaggtg cttcagtgca ggcagcaggt cgctggtggc ggcatgcacg
     3781 gccatgaccc ggatcgccgt cgggaccgcg tcgttgctgc tctggcccag gttgacgtgg
     3841 tcgttgggat ggatcgccag cccggaggcg cgggccgcca ggtgcgcgac cacctcgttc
     3901 gcattcatgt tgctggaggt gccggaaccg gtctggtagc ggtccagcgg gaactgcccg
     3961 tcgtggccgc cgtcggcgac cgcgcgcgca gcggtggcga tggcctcggc acgcgccgca
     4021 tccagcagcc ccagcctgcc gttgaccgcg gccgccgccc ccttgacctg cgccagcgca
     4081 cgcacgaagc cggccggcat cggccgcccc gagacccgga agttgtccag cgcgcgttgc
     4141 gtctgcgcgc cccacagcgc ctcggcgggc acctgcaggt cgcccatgct gtcgtgttcg
     4201 atccggaatc cgctgcgcct ggccacgact cgctcccgcc gatgtccacc cgagcatagc
     4261 gccgccgggg tttagaattg cgcattcccc acgcagctgc cgccccatgc ctgacgccct
     4321 gaccgccctg tccccgctcg atggccgcta cgccggcaag gtcgatgccc tgcgcccgat
     4381 cttttccgaa tacggcctgg tccgcgcgcg ggtgaaggtg gaggtggaat ggttgctggc
     4441 gctggcggag gcgcccgcca tcgccggatt gccgcccttc tccgaggccg ccgcggcgcg
     4501 cctgcgcgca ctggccgatg gattctcggt ggccgacgcg gcacgggtga aggagatcga
     4561 gcgcaccacc aaccacgacg tcaaggcggt cgaatacctg atcaaggaac gcctgcagga
     4621 cgatgccgag cttggcccgg cgctggagtt cgtgcacttc gcctgcacca gcgaggacat
     4681 caacaacctg tcctacgcgc tgatgctcaa tcaagcgcgc ctgtcggtgc tgctcccgaa
     4741 gctggatgac ctgatccaga aactgcgggc gatggcgcac gagcatgcgg cgctgccgat
     4801 gctctcccgc acccacggcc agaccgcgtc gccgaccacg gtgggcaagg aactcgccaa
     4861 cgtggtcgcc cgcctgcagc gacagggcga agtgctggcc gggctgccga tgccgggcaa
     4921 gatcaacggc gcggtgggca actacaacgc gcatgtcgcg tcgtacccgg acatcgactg
     4981 gc
//
LOCUS       DAORUK010000150         4873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_21510_length_4873_cov_17.680365, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000150 DAORUK010000000
VERSION     DAORUK010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21510_length_4873_cov_17.680365"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PK818_09040"
     CDS             complement(<1..741)
                     /locus_tag="PK818_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HQY82912.1"
                     /translation="MNRLLDDRATRLFIGLAAFFCVNAVLAEFIGVKIFALEDTLGIA
                     PLQWNLFGQSGSLNFTAGTLLWPIVFIMTDTVNEYFGKRGVRLISWLAAGFIVYGFVF
                     AFVAIYLAPASWWIGVARDQGVPDYQAAFAAVFGQGLWTIAGSLVAFMIGQLIDVRVF
                     HRIRRATGERQAWLRATGSTAVSQLVDSFVVLYIAFVLGPQQWPTSLFLAVGTLNYAY
                     KMLAAIALIPLLYLMRAGIHRYLGKERAA"
     gene            complement(738..1418)
                     /locus_tag="PK818_09045"
     CDS             complement(738..1418)
                     /locus_tag="PK818_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010380413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HQY82913.1"
                     /translation="MSTSPTPLGPRLQRLFLTGLLTLLPLWLTWVVVKFVFVLLSDVS
                     RPLVEPSLATIATQNPHSFGWLVEPWVLGAIGLVATVCTILLVGWLARRVIGQRLLRW
                     FEALVARIPLANTIYGSARKLLDILQTKPDGTQRVVLIDFPHAEMKTLGFVTRVIREA
                     GTGRELAAVYVPTTPNPTSGYLEIVPMEKITPTDWTVDQAMSFIISGGAVAPEFIPFS
                     PPAHAPKP"
     gene            complement(1450..3429)
                     /locus_tag="PK818_09050"
     CDS             complement(1450..3429)
                     /locus_tag="PK818_09050"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HQY82914.1"
                     /translation="MSSIEQPQIDGYRILRVVGHGGMSTVYLAEQASLGRKVALKVML
                     PEALADEVSRARFENEARTIARLQHPHIVGIHEVGRTADGLPFYSMPYLPRGHLAQRN
                     LRGDQQKVAAILRAMLAALDFAHVRGVVHRDVKAENVLFDENERPMLADFGIALRRGN
                     NPRLTSTGLAVGSTAYMPPEQARGREVDRRADIYSIGVLAWEMLTGRLPFNAGDALSM
                     ALKHVQDPIPRLPPVLRHWQGFIDKSLAKQPADRYASAQEMLDALDELERRAGRNFGP
                     VEVPAPTDDDAPVQPARRAGWKPWALALAAVAALAFGAHALRGSRTDTLPASEDVARA
                     QAPAARGEALPAPAADPQTGLSEAAPGADASGIGAYLANAEQQLREGHLLAPANGNAW
                     DSLDAAWRVDATHPQTQMLTARVFDALADLGERSLGDGDLDAARTAFARARELDARRG
                     GDGSAIALLRKRLDGALGKRLDMLVARQDRAGAEALLAGTRWLGLEAPRSAALQARVA
                     GMAKAKATATTSAPASAAPVAKAGADVRTVSRGDYARFVAETSRASAECGRNGLFGGK
                     RDWKTAGNEGSPVVCVSAADAQAYAAWLSSRDKRRYRLPSAGELRAQATSPVSGWLTL
                     CADAGCRKRMASGKARALEAGRGYADVGIRLVREG"
     gene            complement(3491..3940)
                     /locus_tag="PK818_09055"
     CDS             complement(3491..3940)
                     /locus_tag="PK818_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HQY82915.1"
                     /translation="MNPNTLRHGMNLWPPFLFAGIHVTEISPDWRRVRVELRARPWNR
                     NFVGTHFGGSLFAMTDPFRMIPMLHCLGRDYIVWDKAGEIEFVKPGRGTMHATFELDD
                     AVLDALRAATANGEKVLRWFETDVVDADGEVVARTRKQLYVRRKPGR"
     gene            complement(4006..>4873)
                     /gene="trxA"
                     /locus_tag="PK818_09060"
     CDS             complement(4006..>4873)
                     /gene="trxA"
                     /locus_tag="PK818_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQY82916.1"
                     /translation="DTATPHVFDATAATFEDTVIRKSLETPVLVDFWATWCGPCKTLG
                     PLLDKLAGDYNGAFVLAKVDVDQEQELAAAFQIRSVPTVMLVKDGQLVDGFPGVLPEG
                     QLREFLKHHGIEPAALAEQAAAVETAPPLDPHAEVMRLRKAIEAEPDKSELALDLALA
                     LLQTGAAGEAEHLLDGLPANLATDDRTVKARARLGFAALLQDAPPAAVLETAIAADPA
                     DLRARHLLGVRRLVSGDAEAGLQQFIEMLQRDRGFGDGLAKKSLIDAFRVVEDEDLVG
                     RYRRKMASLLLV"
BASE COUNT          704 a         1684 c         1761 g          724 t
ORIGIN      
        1 cgccgcgcgt tccttgccga ggtagcggtg gatgcccgcg cgcatcaggt acagcagcgg
       61 gatcagggca atcgccgcca gcatcttgta ggcgtagttg agcgtgccga cggccaggaa
      121 cagcgaggtc ggccattgtt gcggacccag cacgaaggcg atgtagagca ccacgaagct
      181 gtccaccagc tgcgagaccg cggtggaacc ggtcgcacgc aaccaggcct ggcgttcgcc
      241 ggtggcgcgc cggatccggt ggaacacgcg gacgtcgatc agctggccga tcatgaaggc
      301 gaccagtgag cccgcgatcg tccacaggcc ttgcccgaac accgccgcga acgcggcctg
      361 gtaatccggc acgccctggt ccctggccac gccgatccac caggaggcgg gcgccaggta
      421 gatggcgacg aaggcgaaca cgaagccata cacgatgaac ccggcggcca gccagctgat
      481 caggcgcacg ccgcgcttgc cgaagtactc gttgacggtg tcggtcatga tgaacacgat
      541 cggccacagc agggtgccgg cggtgaagtt gagcgaaccg ctctggccga acaggttcca
      601 ctgaagcggc gcgatgccga gggtgtcttc cagcgcgaag atcttgaccc cgatgaactc
      661 ggcaagcact gcgttgacgc agaagaacgc agccaggccg atgaacagcc gggttgcgcg
      721 gtcgtcgagc agccggttca cggcttcggc gcgtgcgccg gcggcgagaa cgggatgaac
      781 tcgggggcga ccgcgccacc gctgatgatg aagctcatcg cctggtcgac ggtccagtcg
      841 gtcggcgtga tcttttccat cggcacgatc tcgaggtaac cggaggtcgg gttcggcgtg
      901 gtcggtacgt agaccgcggc cagctcgcgg ccggtgcccg cctcgcggat gacccgggtg
      961 acgaagccga gggtcttcat ctccgcgtgc gggaagtcga tcaggaccac gcgctgggtc
     1021 ccgtccggct tggtctgcag gatgtcgagc agcttgcgtg cgctgccgta gatcgtgttc
     1081 gccagcggga tgcgggccac cagcgcctcg aaccacctga gcaggcgttg cccgatcacc
     1141 cgccgggcca gccagccgac cagcaggatg gtgcacacgg tcgcgaccag cccgatcgca
     1201 cccagcaccc acggctcgac cagccagccg aacgaatgcg ggttctgggt cgcgatcgtc
     1261 gccagcgacg gttccaccag gggccggctg acgtcgctca gcagcacgaa cacgaacttg
     1321 accaccaccc aggtcagcca cagcggcagc agggtcagca gcccggtgag gaacaggcgt
     1381 tgcaggcgcg ggccgagcgg ggtgggggaa gtcgacatgc gcgccattgt aggggcgatg
     1441 ggcgcggcgt cagccctcgc gcaccaggcg gataccgaca tcggcgtagc cacggccggc
     1501 ctcgagggcg cgtgccttgc cactggccat gcgcttgcga cagcctgcgt ccgcgcacag
     1561 ggtcagccag ccggagaccg gcgaggttgc ctgcgcgcgc agttcgccgg cgctgggcag
     1621 gcgatagcgg cgcttgtcgc ggctgctcaa ccacgcggca tacgcctgcg cgtccgcggc
     1681 ggacacgcag accaccggtg agccctcgtt gcccgcggtc ttccagtcgc gcttgccgcc
     1741 aaacaggccg ttgcggccgc attccgcgga tgcgcgcgag gtctcggcga cgaaccgtgc
     1801 gtagtcgcca cggctcaccg tgcgcacgtc ggcgccggcc ttcgccaccg gggcggcgct
     1861 ggcgggggcg ctggtggtcg cggtcgcctt cgccttcgcc atgcccgcga cccgtgcctg
     1921 cagggccgcg ctgcgtggtg cctccagccc cagccagcgg gtcccggcga gcagggcttc
     1981 ggcgccggcg cgatcctgtc gcgcgaccag catgtccagc cgcttgccaa gggcgccgtc
     2041 cagccgcttg cgcagcagcg cgatcgcact gccgtcgccg ccgcgacgcg catccagttc
     2101 gcgcgcgcgc gcgaacgcgg tgcgggcggc gtcgaggtcg ccatcgccca ggctgcgctc
     2161 gccgaggtcg gccagtgcgt cgaacacgcg cgcggtcagc atctgcgttt gcgggtgggt
     2221 ggcatcgacc cgccacgccg cgtccaggct gtcccaggca ttgccgttcg ccggtgcgag
     2281 caggtggcct tcgcgcaact gctgctcggc gttggcaagg taggcgccga ttccgctggc
     2341 gtccgcgccg ggtgcggcct cggacagccc ggtctgcggg tccgccgccg gcgcaggcag
     2401 ggcctcgccg cgcgcggcgg gcgcttgcgc gcgggcgacg tcctcgctcg ccgggagcgt
     2461 gtcggtgcgg ctgccgcgca gcgcgtgggc accgaacgcg agcgcggcca ccgccgccaa
     2521 ggcgagcgcc cacggcttcc agccggcgcg gcgcgcgggt tgcaccggtg cgtcgtcgtc
     2581 ggtgggcgcc gggacctcga ccgggccgaa gttccggccc gcgcgccgct ccagctcgtc
     2641 gagcgcatcc agcatctcct gcgcactggc ataacggtcg gcgggttgct tcgccaatga
     2701 cttgtcgatg aagccctgcc agtgcctgag caccgggggc aggcgcggaa tcgggtcctg
     2761 cacgtgcttg agcgccatcg acagcgcatc gccggcgttg aacggcagcc gcccggtgag
     2821 catctcccag gccagcacgc cgatgctgta gatgtcggcg cgacggtcga cctcgcgtcc
     2881 gcgcgcctgt tccggcggca tgtaggcggt gctgccgacc gcgagcccgg tcgaggtcag
     2941 ccgcgggttg ttgccgcggc gcagggcgat gccgaagtcg gcgagcatcg gccgttcgtt
     3001 ctcgtcgaac agcacgttct ccgccttgac gtcgcggtgg accacgccgc gcacgtgcgc
     3061 gaagtccagt gccgccagca tcgcgcgcag gatcgccgcg accttctgct ggtcgccgcg
     3121 caggttgcgc tgggccaggt gcccgcgcgg gaggtagggc atcgaataga acggcagccc
     3181 gtcggcggtg cgcccgacct cgtggatgcc gacgatgtgc gggtgctgca ggcgggcgat
     3241 ggtgcgcgcc tcgttctcga agcgcgcgcg gctcacttcg tcggccagcg cttccggcag
     3301 catcaccttg agcgcgacct tgcgtcccag cgaagcctgt tcggccaggt acacggtgga
     3361 catgccgccg tggccgacca cgcgcaggat ccggtagccg tcgatctgcg gttgttcgat
     3421 gctggacaag ggcgcttccc ccgcgggtgt cggcgaagga tacgccgcgc gcccgcggcg
     3481 atggaaccgg tcagcgccct ggcttgcggc gcacgtacag ctgcttgcgg gtgcgcgcga
     3541 cgacctcgcc gtcggcgtcc accacgtcgg tttcgaacca gcgcaggacc ttttcgccgt
     3601 tggcggtggc cgcgcgcagc gcgtccagca cggcgtcatc cagttcgaag gtggcgtgca
     3661 tggtgccgcg ccccggcttc acgaattcga tttcgccggc cttgtcccag acgatgtagt
     3721 cgcggcccag gcagtgcagc atcgggatca tccggaacgg gtcggtcatc gcgaacaggc
     3781 tgccaccgaa gtgggtgccg acgaaattgc ggttccacgg ccgcgcccgc agttccaccc
     3841 gcacccgccg ccagtccggc gagatctcgg tgacatggat gccggcgaac aggaacggcg
     3901 gccacaggtt catcccgtgg cgcagggtgt tggggttcat cgcggcagcg cgtcggtgcg
     3961 ggcgcgccag cttaccatac gcaaaggtat ggtcttgccg ccgggtcaga ccagcagcag
     4021 cgaggccatc ttccggcgat agcggccgac caggtcttcg tcctcgacca cgcggaaggc
     4081 atcgatcagc gatttcttcg ccagcccgtc gccgaagccg cggtcgcgct gcagcatctc
     4141 gatgaactgt tgcaggccgg cttcggcatc gccggagacc agcctgcgca cgcccagcag
     4201 gtggcgcgcg cgcaggtcgg cggggtcggc ggcgatcgcg gtctccagta ctgcggccgg
     4261 cggtgcgtcc tgcagcagtg cggcgaagcc gaggcgcgcg cgcgccttca ccgtgcggtc
     4321 gtcggtggcg aggttggcgg gcaggccgtc cagcaggtgc tcggcttcgc cggcggcccc
     4381 ggtctgcagc agtgccagcg ccaggtccag cgcgagttcg gacttgtcgg gttcggcctc
     4441 gatggccttg cgcaggcgca tcacttccgc atgcggatcg agcggcggcg cggtctccac
     4501 cgcggcggct tgttccgcca gcgcggccgg ctcgatgccg tggtgcttca ggaattcgcg
     4561 cagctggcct tccggcagca cgccggggaa gccgtccacc agttgcccgt ccttgaccag
     4621 catcaccgtc ggcaccgagc ggatctggaa cgcagccgcg agttcctgct cctggtcgac
     4681 gtcgaccttg gccagcacga acgcgccgtt gtagtcgccg gccagcttgt ccagcagcgg
     4741 gcccagggtc ttgcaggggc cgcaccaggt ggcccagaaa tccaccagca ccggggtctc
     4801 cagcgacttg cggatgaccg tgtcctcgaa ggtcgcggcg gtggcgtcga agacgtgcgg
     4861 agtggcggtg tcg
//
LOCUS       DAORUK010000151         4868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_21547_length_4868_cov_15.393102, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000151 DAORUK010000000
VERSION     DAORUK010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21547_length_4868_cov_15.393102"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..512
                     /locus_tag="PK818_09065"
     CDS             207..512
                     /locus_tag="PK818_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82917.1"
                     /translation="MDLPDRFRVSTVDPAWSARIEGSAQVVLDGPGGQHRVLRVEMHE
                     AVFDGRYVQARDAQGMVEIRVTARLCRQDGSATADPYTARLTIEGSAPLMGCGQPLQ"
     gene            616..>3003
                     /locus_tag="PK818_09070"
     CDS             616..>3003
                     /locus_tag="PK818_09070"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQY82918.1"
                     /translation="MSVATLIRNLACALLLAAWMPGTAQEGLATADPTAPSAGEASGE
                     PAASPRAIPLAEITDRADADERFAKATRSQAGAIDFGAELGPRLDALRASVTSKQRGA
                     GDASLRMVPILRLESLSRHWSFDARQFERWKADYKATSEPLADGADQLSRRIADWEAT
                     RAALAPGSLPQALSARIDAVARELRAAQQALSAPLAAQIALGRRANLLEAEIQAGQAA
                     VDRAIADIDKRLLRRDAGVIWHAATAPDRASGHAASTRALELETHFLGQYADAGFPNQ
                     RILQFLQLLLLPLLLWLAWRVRIRPPTADVNAAQPLRRPISCWVLLAMIGVLVFEPEA
                     PLLMHQLAMALVLVPVLRLLPAEIGGRLGWWPHVVILQYLLLRLALLPLAGTGLFRWA
                     LLGLAVLVIAATLVLSWWVRRNRQPADRGGLERTVLAAGWLVVGALGVSVLANLLGNV
                     SLAEMLLSGVVESGYVALLVHAAYTVFAALLREVFSSRFAAPLGRAQVGGTTLLALLL
                     RLLAVAAVLGWLVSAMQEFRVYRPMSGLLQALLTHQVAVGELSLSLGDVAVFVIAVFL
                     AFWVASLVKVLLSEELLPRMALADGVGHSIAALTYYAVLMLGLLGALSAAGFKLSQLS
                     LLIGALGVGIGLGLQDVVKNFVSGLILMFERPLKPGDVVDIAGTTGRVHEIGMRATTL
                     RTFDGADVVVPNGTLLADKFTNWTLRDRGKRIDVDVGIAYGTDAALAIRVLERATRET
                     PKVAAEPAPAVLFLGLGDSALNFGVRAWTDEFDDSPKVRSDLVARLYAALGEAGIE"
     assembly_gap    3004..3103
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3104..3700)
                     /locus_tag="PK818_09075"
     CDS             complement(<3104..3700)
                     /locus_tag="PK818_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82919.1"
                     /translation="MKSRLSLAIATLAAGIGLAIGPVNVALAQNASQLGDLVGARAAG
                     GESDLEARGYTFIQGGDAYGDAKQAYYWNGRNKACVRVETYDGRFRSITNADAAQCNQ
                     RSSKGSDNGKAAMAILGLAAIAAAAAHQDNQHSNASNNSAQWDIGYNDGLHGVPYHNV
                     ARSDGYSGGYDAGVRQRNQNTSYHSGRGGYAQAARFKDL"
     gene            complement(3762..4223)
                     /locus_tag="PK818_09080"
     CDS             complement(3762..4223)
                     /locus_tag="PK818_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82920.1"
                     /translation="MNAPARFIVVTATAIALLPLAAQATDPAYRKKLERSGCTQVTEL
                     QGCDINKTKAENAKAGFGTAPKPAVEAASPYSGQWIARSTSGLTVATIGIDAKERVTV
                     NGKAVTARRSDGALVFKVGFITYTLQGDPRLANESSWFDSDAQTTGPIRRK"
     gene            complement(4452..>4868)
                     /locus_tag="PK818_09085"
     CDS             complement(4452..>4868)
                     /locus_tag="PK818_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009594336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HQY82921.1"
                     /translation="RYLLIGTGTGITPYRAMLPLLDRLIAARGVEVVLLQGARTPAEL
                     LYGDEFRAFANAHPGFRYLPCLSRELPAAGSPHAHAEVRHGYVQDALAELAPDAEGDI
                     AYLCGNPDMVDACFEALKGAGLPVPQVRREKYVSNK"
BASE COUNT          628 a         1590 c         1757 g          793 t
ORIGIN      
        1 ccgggcagga cagtcgcttg cctgcaagct tgtgtaggat cgaagcatcg ctacatgcag
       61 gcaaggccat gcgcagcgcg ttggcgacga tcctcctggt gggccatctc gcggcctgcg
      121 gcaccgcgac ccgatcgcct tcgccgcccg cgccgcccgc gccgggaaca ggcgccacgc
      181 aaccggcgcc ggtcctggat gccccggtgg acctgcccga tcgcttccgg gtgtccaccg
      241 tcgatcccgc gtggtcggcg cgcatcgaag ggtcggcgca agtggtgctg gatggaccgg
      301 gcggccagca ccgggtgttg cgggtcgaga tgcacgaggc cgtcttcgac gggcgctacg
      361 tgcaggcgcg cgatgcgcag ggcatggtcg agatccgggt gacagcgcgg ctgtgccggc
      421 aggacggctc tgccaccgcg gatccataca cggcgcggct caccatcgaa ggcagcgcgc
      481 cactgatggg gtgcgggcag cccttgcaat gaatgccatg ccggggcgtg cgcaggcgcg
      541 atgcacgcag ggttgcagtg cgcttccgga atccccgatc ctgatccccg atcccccgcc
      601 ccaggccgac ccgccatgtc cgtcgccacc ctgatccgca acctcgcctg cgcgctgctg
      661 ctggcggcat ggatgcccgg cactgcgcag gagggacttg cgacggccga cccgacggca
      721 ccgtccgccg gggaagcgag cggggagccc gcagcgtcgc cgcgtgccat tccgttggcc
      781 gagatcaccg atcgggccga cgcggacgag cgattcgcca aggcgaccag gagccaggcc
      841 ggggcgatcg atttcggcgc cgaattgggc ccgcgcctgg atgcgcttcg cgcctcagtc
      901 accagcaagc agcgtggcgc cggcgatgcg agcctgcgga tggtgcccat cctccgcctg
      961 gaaagcctgt cccggcactg gtcgttcgac gcgcgccagt tcgagcgatg gaaggcggat
     1021 tacaaggcca ccagcgaacc cctggccgac ggcgcggacc agctgtcccg gcgcatcgcg
     1081 gattgggagg ccacccgtgc ggcactggcg cccggcagcc tcccgcaggc gttgtcggcg
     1141 cggatcgatg cagtcgccag ggaattgcgc gccgcacagc aggcgttgtc ggcaccgttg
     1201 gccgcgcaga tcgcgctcgg tcgtcgcgcc aacctgctgg aggctgagat ccaggccggc
     1261 caggccgcgg tggaccgcgc catcgccgac atcgacaagc gcctgttgag gcgcgatgcc
     1321 ggggtcatct ggcatgccgc cacggcgccc gaccgcgcca gcggacacgc cgccagtacc
     1381 cgcgcccttg agctggagac acatttcctg gggcaatatg cggacgccgg gttccccaac
     1441 cagcgcatct tgcagttcct ccagctgctc ctgctgccgc tgctgctgtg gttggcctgg
     1501 cgcgtgcgga tccgcccgcc cacggccgac gtcaatgcgg cccagccgct gcgacgcccg
     1561 atctcgtgct gggtgctgct ggcgatgatc ggggtgctgg tgttcgaacc cgaggcgccg
     1621 ctgctgatgc accagctggc gatggcactg gtactggtcc cggtgttgcg cctgttgccc
     1681 gcggagatcg gcggacgcct gggctggtgg ccacacgtgg tgatcctgca gtacctgctg
     1741 ctgcggcttg cgttgctgcc gctggccggg accgggctgt tccgctgggc cttgctgggc
     1801 cttgccgtgc tggtgatcgc ggccaccctg gtgctgtcgt ggtgggtccg ccgcaacagg
     1861 cagcccgccg atcgcggcgg gttggagcgg accgtgttgg cggctggctg gctggtggtc
     1921 ggtgcgctgg gtgtctcggt gctggcgaac ctgctgggca atgtgtcgct ggcggaaatg
     1981 ctgttgtccg gggtggtcga gagcggctac gtcgcgttgc tcgtgcatgc ggcctacacc
     2041 gtgttcgccg cgctgctgcg tgaagtcttc agcagccgct tcgcggcacc gctgggacgg
     2101 gcgcaggtcg gtggcaccac cctgctggcg ctgctcctgc ggttgctggc ggttgcagcg
     2161 gtgctgggct ggctggtgtc ggcgatgcag gagttccggg tctacaggcc gatgtcgggc
     2221 ctgttgcagg ccctgctgac ccaccaggtg gccgtcgggg agctgtcgct cagcctcggc
     2281 gatgtcgccg tgttcgtgat cgcggtgttc ctggccttct gggtcgccag cctggtcaag
     2341 gtgctgttga gcgaggagtt gctgccacgg atggcactgg ccgacggcgt gggccacagc
     2401 atcgctgccc tgacctacta cgcggtgctg atgctcggcc tgcttggcgc gctgtccgcc
     2461 gcggggttca agctcagcca gttgtcgctg ctgatcggcg cgctcggcgt cggcatcggc
     2521 ctgggcctgc aggacgtggt caagaacttc gtgtcggggc tgatcctgat gttcgagcgt
     2581 ccgctgaagc cgggcgacgt ggtcgacatc gccggcacca ccggccgcgt gcacgagatc
     2641 ggcatgcgcg cgaccaccct gcgcacgttc gacggcgccg acgtggtggt gcccaacggc
     2701 accctgctgg ccgacaagtt caccaactgg acgctgcgcg atcgcggcaa gcggatcgac
     2761 gtggacgtcg gcatcgccta cggcaccgac gccgcgctgg cgatccgcgt gctcgagcgg
     2821 gccacccgcg agaccccgaa ggtggcggcg gaaccggcgc cggcggtgtt gttcctcggg
     2881 cttggcgaca gcgcgttgaa cttcggcgtg cgggcctgga ccgacgagtt cgacgatagc
     2941 ccgaaggtgc gcagcgacct ggtggcgcgc ctgtacgccg cgctcggcga ggccggcatc
     3001 gagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaggtcc ttgaaccgcg
     3121 ccgcctgcgc atagccgccg cgaccgctgt ggtagctggt gttctggttg cgctgccgaa
     3181 cgccggcgtc gtagcccccg gaataaccgt ccgagcgcgc gacgttgtgg tacggcacgc
     3241 cgtgcaggcc atcgttgtag ccgatgtccc actgcgcgga attgttgctg gcattggagt
     3301 gctggttgtc ctggtgcgcc gccgcggccg cgatcgcggc caggccgagg atcgccatgg
     3361 cggccttgcc gttgtcgctg cccttgctgc tgcgctggtt gcactgcgcg gcgtcggcat
     3421 tggtgatgga gcggaagcgg ccgtcgtagg tctcgacccg gacgcaagcc ttgttccggc
     3481 cgttccagta atacgcctgc ttcgcatcgc cgtaggcgtc gccgccctgg atgaaggtgt
     3541 agccacgcgc ctcgaggtcg gattcgccgc ctgcggcgcg cgcgccgacc agatcgccca
     3601 gctggctggc gttctgggca agggcgacgt tgaccggacc gatcgcaagg ccgatgccgg
     3661 cggcgagggt ggcgatggcg agggagagac gggatttcat ggtgcgctcc tggatggtgc
     3721 gccggtgggc gcggtatggg cgaagcgaga gcgtgcagtg cttacttgcg gcggatcggg
     3781 ccggtggtct gcgcgtccga atcgaaccag ctgctctcgt tcgcgaggcg cgggtcgccc
     3841 tggagggtgt aggtgatgaa gccgaccttg aacaccagcg caccgtcgct gcgcctggcg
     3901 gtcaccgcct tgccgttgac cgtcacccgt tccttggcat cgatgccgat cgtcgcgacc
     3961 gtcagacccg aggtggaccg ggcaatccac tggccgctgt agggcgacgc ggcctcgacc
     4021 gcgggcttgg gcgcggtgcc gaagccggcc ttggcgttct ccgccttggt cttgttgatg
     4081 tcgcagccct gcagttcggt gacctgggta cagcccgagc gctcgagctt cttccggtag
     4141 gccggatcag tggcttgcgc cgccaatggc agcagtgcga tcgcggtggc ggtgacgacg
     4201 atgaaacggg caggtgcatt catggggtgg agctccggtg gggtgcgtgg ggcaatccgc
     4261 aacgcatctg gcgggagcaa ccaggacccc gccccgcacg gtcttgccgg cacggagcat
     4321 ccagcgcctg caacgggcgt gtccatcggc gggaagccga tcccgcatca gcatttcgct
     4381 gacagcggcc gccgcgcggc gcgatcgggc cggatggcgc cgtggacgca gcggacggtc
     4441 cgggccgcgc cctacttgtt gctgacgtac ttctcgcggc gcacctgagg caccggcagg
     4501 ccggctccct tcagcgcctc gaagcaggca tccaccatgt ccgggttgcc gcacaggtag
     4561 gcgatgtcgc cttccgcgtc cggtgcgagt tccgccagcg cgtcctgcac atagccgtgg
     4621 cggacctcgg catgcgcatg cggggaacct gccgcgggca actcgcgcga caggcacggc
     4681 aggtagcgga accccgggtg ggcgttggcg aaggcgcgga actcgtcgcc gtacaggagt
     4741 tccgcggggg tgcgcgcgcc ctgcagcagc accacctcga ccccgcgcgc ggcgatcagc
     4801 cggtcgagca gcggcagcat cgcccggtac ggggtgatgc cggtgccggt gccgatcagc
     4861 aggtagcg
//
LOCUS       DAORUK010000152         4853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_21649_length_4853_cov_19.970821, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000152 DAORUK010000000
VERSION     DAORUK010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21649_length_4853_cov_19.970821"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1019)
                     /locus_tag="PK818_09090"
     CDS             complement(3..1019)
                     /locus_tag="PK818_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III"
                     /protein_id="HQY82922.1"
                     /translation="MLFQHVAIAGLAHIDAPRRLTSVEINARLRPTMERLGIKTDVLG
                     EIAGIHSRFLWEDNVLASDVATLAGIKALADAGIDPARVGLLVNTSVSRDYLEPSTAS
                     IVSGNLGLSDTCQNFDVANACLAFINGMDIASRMIERGEIDYALIVDGETANLAYEKT
                     LERLGREDATEEQFRNELATLTLGSGAAAMVLARAELAPGAPRYKGGVTRAATEWNKL
                     CRGNLDGMVTDTRMLLIEGIKLAQKTFAAAKLALGWASEELDQFVIHQVSKVHTAAFT
                     KAIGIDPKKVMTIFGEHGNIGPASVPIVLSKLRELGRLKKGDRVALLGIGSGLNCSMA
                     EVVW"
     gene            complement(1127..1438)
                     /locus_tag="PK818_09095"
     CDS             complement(1127..1438)
                     /locus_tag="PK818_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HQY82923.1"
                     /translation="MKVKLLASMLAFLLTACTFVHMAPGATGVKVLATAPANCQKRGE
                     VAVSVKDRLGPYERSEAQVRDELETLARNEAPGLGADRISPLSPVDDGEQRWALWRCG
                     R"
     gene            complement(1567..4398)
                     /locus_tag="PK818_09100"
     CDS             complement(1567..4398)
                     /locus_tag="PK818_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQY82924.1"
                     /translation="MSLFRPAALALALATALSAGAYVAPLQAANAPSVDIPHDSFTLP
                     NGLRVVVHTDRKAPVVAVNIWYHVGSKDEPSGRSGFAHLFEHLMFNGSENNPGEFFAP
                     FKQVGVTDQNGTTNTDRTNYFQNVPTTALDMALWMESDRMGHLLGAVDQATLDEQRGV
                     VQNEKRQGMNQPYGQLREVTTYTLYPKGHPYHHSVIGSMNDLNAAKLADVQAWFKTWY
                     GPNNAVLVLAGDIDLATAREKVTRYFGHIPASPTMAQPKVDVAVLEQDGRTVLEDKVP
                     QVMVRRMWNVPQTGTRDADMLDLFSDVLGGSASSRLDRRLVHQDKLVDSIGTANWASQ
                     LGGNFMVNAMVKQGVDPAKVEAAIDEELKKLIAEGPTAEELARAKTSYEAGFVRGVER
                     IGGFGGKADVLASCAIYERNPGCFRDSLQAVAGATPAQVRDAAAKWLGKPSHVFMVKP
                     GARKELAEAPAGTPSPFVLPKVDARYKTTASSVDRSKGVPVTASFPDLKFPAQQRATL
                     SNGTTVILAERHDVPVVQMSYQFAGGYSADPAGKPGIANFTASLLDEGAGDLDALGFA
                     ARKEALGAQLGAGASLDGNNAILSALKKHLEPSVALFADMLRKPRFDQAQVDRVKGEW
                     IASIRQEKAQPSGVALRVLPALMYGAGHPYGNPLSGTGTEAAIAALTRDDLVAFHQRM
                     MQPAGATLVVVGDTTLAEIVPLLEKHLGSWKVGSAGGAPAIPAVANARQSRVFLIDQP
                     GAVQANIFAAQLVPSSKDPGAIAFDMANQVFGGDFTARLNMNLREDKHWSYGARSGAA
                     SAVGQRMWRATAPVQIDKAGESVAEIRREIAEFFGGSRGTSAEELARQQKGLTLSLPG
                     AYETAGSVLATIGSNVLYGRPDDYVFRRKAEIEAITPAQVDAAAKTLDPGAITWVVVG
                     DLAKTEAPIRALKLGEVTILDAEGKPVSK"
     gene            4504..>4853
                     /locus_tag="PK818_09105"
     CDS             4504..>4853
                     /locus_tag="PK818_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY82925.1"
                     /translation="MRRNCLVTGANRGIGLEFVRQLLARGEHVVAACRHPGKATALNG
                     LAGEHPGRLHVLPLDVAEPKSRANLLHELPLVLAEEEDGSPGKLDLVVNNAGVLHSGE
                     RFGHMEQALLEDSLR"
BASE COUNT          681 a         1626 c         1713 g          833 t
ORIGIN      
        1 gctcaccaga caacttccgc catcgagcag ttcaggcccg aaccgatgcc cagcagggcc
       61 acccggtcgc ccttcttcag gcgcccgagt tcgcgcagct tggacagcac gatcggcacg
      121 ctggccggac cgatgttgcc gtgctcgccg aagatggtca tgaccttctt cgggtcgatg
      181 ccgatcgcct tggtgaaggc ggcggtgtgc accttgctga cctggtggat cacgaactgg
      241 tccagttcct cggaggccca gcccagtgcc agcttggccg cggcgaatgt cttctgcgcc
      301 agcttgatgc cttcgatcag cagcatgcgg gtgtcggtga ccatgccgtc gaggttgccg
      361 cggcacagct tgttccactc ggtggcggcg cgggtgacac cgcccttgta gcgcggcgcg
      421 cccggcgcca gttcggcgcg cgccagcacc atcgcggcgg caccggatcc cagggtcagg
      481 gtggccagtt cgttgcggaa ctgctcttcg gtggcgtctt cgcggcccag ccgttcgagg
      541 gtcttctcgt aggcgaggtt ggcggtttcg ccgtccacga tcagtgcgta gtcgatctcg
      601 ccgcgctcga tcatgcggct ggcgatgtcc atgccgttga tgaaggcgag gcaggcattg
      661 gccacgtcga agttctggca ggtgtccgac aggcccaggt tgccgctgac gatggaggcg
      721 gtggacggct ccaggtagtc gcggctgacc gaggtgttga ccagcaggcc cacccgggcc
      781 gggtcgatgc cggcatcggc gagcgccttg atcccggcga gggtggcgac gtccgaggcc
      841 agcacgttgt cttcccacag gaagcgcgaa tggatgccgg cgatctcgcc cagcacgtcg
      901 gtcttgatcc cgaggcgctc catggtgggg cgcaggcggg cgttgatttc gaccgaggtc
      961 agccggcgcg gcgcgtcgat atgggcaaga ccggcaatgg cgacatgttg gaacagcatg
     1021 ggcgatggct ttctgcgagg gcgccggtgg cgcatgaacc gcgcaggata acggttttca
     1081 cgtcagtacg ctggcgtact ggccgaaaag gtggccgctt ttcgcctcag cgcccgcagc
     1141 gccacagcgc ccagcgttgt tcgccgtcgt ccaccggcga cagcgggctg atgcggtcgg
     1201 cgcccaggcc cggggcttcg ttgcgggcca gggtctccag ttcgtcgcgg acctgcgcct
     1261 cgctgcgttc gtacgggcca agccggtcct tgaccgatac cgccacttcg ccgcgcttct
     1321 ggcaatttgc cggggcagtg gccagcacct tcacgccggt tgcacccggg gccatgtgca
     1381 cgaaggtaca ggcggtcaac aagaacgcga gcatcgatgc aagcaacttc actttcatgc
     1441 gggtctcctc ccggcccacc ggcttgcatg ccggtgggcg ttcgcaggct cgcagctacg
     1501 ctgctcgaaa accctgctca cccagcagtg tggccggcat cgtgcgtgcg tggctgaacg
     1561 gatcgcttac ttcgaaaccg gcttgccctc ggcgtcgagg atggtcacct cacccagctt
     1621 cagggcgcgg atcggggcct cggtcttggc caggtcgccg accaccaccc aggtgatcgc
     1681 gcccggatcc agggtcttcg ccgcggcatc gacctgggcc ggggtgatcg cctcgatctc
     1741 cgccttgcgc ctgaacacgt agtcgtccgg gcggccgtac agcacgttgc tgccgatggt
     1801 ggccagcacc gagccggcgg tctcgtaggc gccgggcagg ctcagggtca ggcccttctg
     1861 ctggcgcgcc agttcctccg cgctggtgcc gcgcgaaccg ccgaagaact cggcgatctc
     1921 gcgccggatc tcggccacgg attcgccggc cttgtcgatc tgcaccggtg ccgtggcacg
     1981 ccacatgcgc tggccgaccg cgctggcggc gccgctgcgc gcgccgtacg accagtgctt
     2041 gtcctcgcgc aggttcatgt tcaggcgcgc ggtgaagtcg ccgccgaaca cctggttggc
     2101 catgtcgaag gcgatcgcgc ccggatcctt gctggacggc accagttgcg ccgcgaagat
     2161 gttggcctgc accgcgcccg gctggtcgat caggaacacc cgcgactgcc tggcgttggc
     2221 caccgccggg attgccggcg cgccgccagc gctgccgacc ttccagctgc cgaggtgctt
     2281 ctccagcagc ggcacgattt cggcgagggt ggtgtcgccg accacgacca gggtcgcgcc
     2341 cgcgggctgc atcatgcgct ggtggaaggc caccaggtcg tcgcgggtca gcgcggcgat
     2401 cgcggcctcg gtgccggtgc cgctgagcgg gttgccgtac gggtgcccgg cgccgtacat
     2461 cagcgccggc agcacgcgca gggcgacgcc gctcggctgc gccttctcct gcctgatgct
     2521 tgcgatccac tcgcccttca cgcggtcgac ctgggcctgg tcgaagcgcg gcttgcgcaa
     2581 catgtcggcg aacagcgcca ccgatggctc caggtgcttc ttcaatgccg acaggatggc
     2641 gttgttgccg tccagcgatg cgccggcgcc gagctgcgcg ccgagtgctt ccttgcgcgc
     2701 ggcgaagccg agggcgtcga ggtcgccggc accttcgtcg agcaggctgg cggtgaagtt
     2761 ggcgatgccg ggcttgccgg ccgggtccgc cgagtaaccg ccggcgaact ggtagctcat
     2821 ctgcaccacc gggacgtcgt ggcgttcggc caggatcacg gtggtgccgt tggacagggt
     2881 ggcgcgctgc tgggccggga acttcaggtc cgggaagctg gcggtgaccg gtacgccctt
     2941 gctgcggtcg acgctgctgg cggtggtctt gtagcgggca tcgaccttgg gcagcacgaa
     3001 cggcgacggg gtgccggccg gggcctcggc cagttccttg cgcgcgcccg gcttcaccat
     3061 gaacacgtgg ctcggcttgc ccagccactt cgccgccgca tcgcgcacct gcgccggggt
     3121 ggcaccggcc accgcctgca gggaatcacg gaagcagccc gggttgcgct cgtagatcgc
     3181 gcacgaggcc agcacgtcgg ccttgccgcc gaacccgccg atgcgctcga cgccgcggac
     3241 gaagccagct tcgtagctgg tcttggcgcg cgccagttcc tcggcggtgg ggccttcggc
     3301 gatcagcttc ttcagctcct cgtcgatcgc cgcttccacc ttggcggggt cgacaccctg
     3361 cttgaccatc gcgttgacca tgaagttgcc gcccagctgc gaggcccagt tggcagtgcc
     3421 gatgctgtcc accagcttgt cctggtgcac caggcgccgg tccagccgcg agctggcact
     3481 gccgccaagc acgtcggaga acaggtccag catgtcggcg tcgcgggtgc cggtctgcgg
     3541 cacgttccac atccgccgca ccatcacctg cggcaccttg tcctccagca cggtgcggcc
     3601 atcctgctcc agcaccgcca catcgacctt gggctgggcc atcgtcgggc tggccgggat
     3661 atggccgaag tagcgggtga ccttctccct ggcggtggcc aggtcgatgt cgccggccag
     3721 caccagcacc gcgttgttgg ggccgtacca ggtcttgaac caggcctgca cgtccgcaag
     3781 cttggccgcg ttgaggtcgt tcatcgagcc gatcaccgaa tggtggtacg ggtggccctt
     3841 cgggtacagg gtgtaggtcg tcacttcgcg caactggccg tagggctggt tcatgccctg
     3901 gcgcttctcg ttctggacga cgccgcgctg ctcgtcgagg gtggcctggt cgaccgcgcc
     3961 cagcaggtgg cccatgcggt cggattccat ccacagcgcc atgtccagcg cggtggtcgg
     4021 cacgttctgg aagtagttgg tgcggtcggt gttggtggtg ccgttctggt cggtgacgcc
     4081 gacctgcttg aacggggcga agaactcgcc cgggttgttc tcggacccgt tgaacatcag
     4141 gtgttcgaac aggtgggcga agccgctgcg gccgctgggc tcgtccttgc tgcccacgtg
     4201 gtaccagatg ttgaccgcca ccaccggtgc cttgcggtcg gtgtgcacga ccacgcgcag
     4261 gccgttcggc agggtgaagc tgtcatgggg gatgtccacg gacggcgcgt tcgcggcctg
     4321 cagcggggcg acgtaggcac cggcggacag cgcggtggcg agggcaagcg cgagcgcggc
     4381 gggacggaac agggacatgc ggaactccgg cgggacggca aagagggccg atgttagcgg
     4441 cggcggcagg ccgatacact aggccaaaag ccactcctga ccttgccctg ccgaggaagc
     4501 cgcatgcgtc gcaactgcct ggtcaccggc gccaaccgtg gcatcggcct ggaattcgtg
     4561 cgccagctgc tggcgcgggg cgagcatgtg gtcgccgcct gccgccaccc gggcaaggcc
     4621 accgcgctga acggcctggc cggcgagcat ccgggccggc tgcacgtgct gccgctggac
     4681 gtggcggagc cgaaatcccg cgccaacctg ctgcacgagc tgccgctggt gctggccgaa
     4741 gaggaggacg gcagccccgg caagctcgac ctggtcgtca acaacgctgg cgtgctgcac
     4801 tcaggggagc gcttcgggca catggagcag gcgctgctgg aagacagcct gcg
//
LOCUS       DAORUK010000153         4821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_21868_length_4821_cov_16.799622, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000153 DAORUK010000000
VERSION     DAORUK010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21868_length_4821_cov_16.799622"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PK818_09110"
     CDS             complement(<1..1126)
                     /locus_tag="PK818_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQY82926.1"
                     /translation="MTAIRPATVAVHAGNQPDAATGAVAAPIHLATTFRHGAAGERLA
                     GYEYQREGNPTNDRLREALAALEGGATACTFASGMAAMTTVLECLPNGARLLVPDDCY
                     TGLRMLCAEFLPERGIRPVSVDMTDLDAVRAACAEPVAMLWIETPSNPLMQVADIAAL
                     AQLGHAHGATVVVDNTFATPLLQRPLALGADIVMHSTTKYFGGHSDVLGGALVFAHDD
                     AFAQKVAHRLHVTGAVLAPFSAWLILRGCRSLGARMAMHCANARKVADFLAAHPAIER
                     VNWPGLASHPGHAVAARQMRDFGGMLSIQLRGGRAAALAMASKLQVFTNATSLGGCES
                     LIEHRASVEGANPRSPQDLLRISVGLEDADDLVADLAQALG"
     gene            complement(1134..1976)
                     /locus_tag="PK818_09115"
     CDS             complement(1134..1976)
                     /locus_tag="PK818_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HQY82927.1"
                     /translation="MFEREHFPAATSGWRHRWYRRIFHHDAADERNFDLWLIVAILAS
                     VAVVVLDSVPAIKARWHGQLYLAEWFFTLLFTAEYVLRLLVVRKPLRYARSFFGVIDL
                     LAILPTFLSLLFPASTSLTVIRALRLLRIFRVLKLVEYSSEAGVLVQALLRSRRKIFV
                     FITTLLTIVVIFGALMYVAEGPEHGFTSIPTAMYWAIVTVTTVGFGDIAPGTPFGRFV
                     TSVLIMIGYSIIAVPTGIYTAELARSMQPKRRVVRCVECGLPEHESDAWHCRKCGRPL
                     PTDD"
     gene            complement(2012..3295)
                     /gene="hemL"
                     /locus_tag="PK818_09120"
     CDS             complement(2012..3295)
                     /gene="hemL"
                     /locus_tag="PK818_09120"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HQY82928.1"
                     /translation="MNSERSHDLFTRAQALMPGGVNSPVRAFKSVGGEPFFVQRADGP
                     YLFDADGNRYIDYVGSWGPMIVGHNHPKVLDAVIETARDGLSFGTPNPLEVTMAETIT
                     RLVPSCEMVRMVNSGTEATLSAIRLARGATGRNRIVKFEGCYHGHGDSFLVKAGSGAL
                     TFGVPTSPGVPKALADLTLTIAYNDFDAATRLFEDCGGDIAALIIEPVVGNANCLPPR
                     DGYLQHLRALCTQHGALLIFDEVMTGFRVALGGAQARYGVTPDLSTFGKVIGGGMPVG
                     AYGGRRELMQQIAPSGPIYQAGTLSGNPVAMAAGLAMLALIQAPGFHDALEARTHALC
                     DGLEAAAREAGVAFSTQRVGGMFGLFFSAEKVDTFAQATACDSAAFNRFFHAMLERGV
                     YLAPSAFEAGFMSAAHDADVIAATVEAARAAFAEL"
     gene            complement(3292..3927)
                     /gene="thiE"
                     /locus_tag="PK818_09125"
     CDS             complement(3292..3927)
                     /gene="thiE"
                     /locus_tag="PK818_09125"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008575802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HQY82929.1"
                     /translation="MPGRGPERGLYLITPDERDGGRLLARTLPLLPFASCLQLRNKVL
                     DAVALRGAGARLRDACAEAGVAFIVNDDPRLARALGADGVHLGEQDGAIAAARALLGA
                     DAIIGASCYDDLQRARDAVAAGADYIAFGAFFPSPTKPGARRAGIGLLRDSAGLGVPR
                     VAIGGITPDNAPPLVAAGADLLAVISGVYDAPEPVAAVRAYLSCFKDEPDT"
     gene            3989..4183
                     /locus_tag="PK818_09130"
     CDS             3989..4183
                     /locus_tag="PK818_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HQY82930.1"
                     /translation="MSEPAAADAPYRTWMCVVCGFVYDEALGLPEDGIAPGTRWDDVP
                     DTWTCPDCGVGKDDFELMRD"
     gene            complement(4193..4639)
                     /locus_tag="PK818_09135"
     CDS             complement(4193..4639)
                     /locus_tag="PK818_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014508843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HQY82931.1"
                     /translation="MRAFALALFAASSLSACASAREPWVELAGQRYAIEVADDDAERA
                     RGLMFRDALPEDRGMLFIHDAEVPQAYWMKNTKIPLDILYFDTGRRLVSQQRDIPPCS
                     LGDACPSYPSNAPARYVLELNAGEAARLQLQDGAELTFGPGIPLAK"
BASE COUNT          764 a         1643 c         1719 g          695 t
ORIGIN      
        1 agcccagcgc ttgcgcgagg tcggcgacca ggtcgtcggc gtcctccagt cccaccgata
       61 tgcgcagcag gtcctgcggc gaacgcggat tcgcgccctc caccgaggcg cggtgctcga
      121 tcagcgattc gcagccgccc agcgaggtgg cattggtgaa cacctgcagt ttcgacgcca
      181 tcgccaatgc agccgcgcgg ccgccgcgca gctggatgct gagcatgccg ccgaagtcgc
      241 gcatctggcg ggcggcgacc gcgtggcccg ggtggctcgc aaggccgggc cagttgaccc
      301 gttcgatcgc cggatgcgcc gccaggaagt ccgcgacctt gcgcgcgttc gcgcaatgca
      361 tcgccatccg cgcgcccagc gagcggcagc cgcgcaggat cagccaggcg ctgaaaggcg
      421 ccagcaccgc gcccgtcaca tgcaggcgat gcgcgacttt ctgcgcgaag gcgtcgtcat
      481 gcgcgaacac cagcgcgcca ccaagcacgt cgctgtggcc accgaaatac ttggtggtcg
      541 aatgcatcac gatgtccgcg cccagtgcga gcggcctctg cagcagcggg gtggcgaagg
      601 tgttgtccac caccacggtc gccccgtggg cgtggccaag ctgcgcgagc gcggcgatgt
      661 cggcgacctg catcagcgga ttcgacggcg tctcgatcca cagcatcgcc accggctccg
      721 cgcatgccgc gcgtaccgcg tcgaggtcgg tcatgtccac cgaaaccggc cggatcccgc
      781 gttccggcag gaactccgcg cacagcatgc gcaggccggt gtagcaatcg tcgggcacca
      841 gcaggcgggc gccgttcggc aggcattcca gcacggtggt catcgccgcc atgccggagg
      901 cgaacgtgca cgcggtcgcg ccgccttcca gcgcggccag cgcctcgcgc aggcggtcat
      961 tggtgggatt gccctcgcgc tggtactcgt aacccgccag ccgttcgccc gccgcgccgt
     1021 ggcggaaggt ggtggcgagg tggatcggcg cggcaaccgc gccggtggcg gcatcgggct
     1081 ggttgccggc gtggacggca acggtggcgg gtcggatcgc ggtcatcggc ggctcaatcg
     1141 tcggtaggaa gggggcgccc gcacttgcgg caatgccagg cgtcggactc gtgttcgggc
     1201 aggccgcatt ccacgcagcg cacgacgcgg cgtttcggct gcatgctgcg ggcgagctcg
     1261 gcggtgtaga tgccggtcgg gaccgcgatg atgctgtagc cgatcatgat cagcaccgag
     1321 gtgacgaagc gcccgaacgg cgtgcccggc gcgatgtcgc cgaagccaac cgttgtcacc
     1381 gtgacgatcg cccagtacat cgcggtgggg atgctggtga agccgtgctc cgggccctcg
     1441 gccacgtaca tcagcgcgcc gaagatcacc acgatggtca gcagggtggt gatgaacacg
     1501 aagatcttgc ggcggctgcg caacagcgcc tggaccagca cgccggcctc gctggagtac
     1561 tccaccagct tgagcacgcg gaagatgcgc agcaggcgca gcgcgcggat caccgtcagc
     1621 gaagtgctgg ccgggaacag cagggacagg aaggtgggca ggatcgccag caggtcgatc
     1681 accccgaaga agcttcgcgc gtagcgcagc ggcttgcgca ccaccagcag ccgcagcacg
     1741 tactcggcgg tgaacagcag ggtgaagaac cattcggcga ggtacaactg gccatgccag
     1801 cgggccttga tcgccggcac gctgtccagc accaccacgg cgacgctggc caggatcgcc
     1861 acgatcagcc acaggtcgaa attgcgctcg tccgctgcgt cgtggtggaa gatgcggcgg
     1921 taccagcggt gccgccagcc cgaggtggcg gccgggaagt gttccctctc gaacaggttg
     1981 cgctgcggcg ggggttcctg ttcgcccacc gctagagttc cgcgaacgcc gcgcgtgccg
     2041 cttccacggt ggcggcgatc acgtccgcgt cgtgcgcggc ggacatgaag ccggcctcga
     2101 acgcggacgg cgccaggtac acgccgcgtt ccagcatcgc gtggaagaag cggttgaacg
     2161 cggcgctgtc gcaggcggtc gcctgggcga aggtatccac cttttccgcg ctgaagaaca
     2221 gcccgaacat gccgcccacc cgctgcgtgc tgaacgcgac gccggcctcg cgcgcggccg
     2281 cctccagccc gtcgcacagc gcgtgcgtcc tcgcttccag cgcgtcatgg aagccggggg
     2341 cctggatcag cgccagcatc gccaggcccg ccgccatcgc caccggattg ccgctgagcg
     2401 tgccggcctg gtagatcggg ccgctgggcg cgatctgctg catcagttcg cggcggccgc
     2461 cgtaggcgcc caccggcatg ccgccgccga tcaccttgcc gaaggtggac aggtccgggg
     2521 tcacgccgta gcgcgcctgc gcgccgccca gcgccacccg gaaaccggtc atcacttcgt
     2581 cgaagatcag cagcgcgcca tgctgcgtgc acagcgcgcg caggtgttgc aggtaaccgt
     2641 cgcgcggcgg caggcagttg gcgttgccga ccaccggttc gatgatcagc gcggcgatgt
     2701 cgccgccaca gtcctcgaac aggcgggtgg cggcatcgaa atcgttgtac gcgatggtca
     2761 gggtcaggtc ggccaaggcc ttgggcacgc ctggcgacgt cggcacgccg aacgtcagcg
     2821 caccgctgcc ggccttgacc aggaagctgt cgccgtggcc gtggtagcag ccttcgaact
     2881 tcacgatccg gttgcgcccg gtggcgccgc gcgccagccg gatcgccgac agggtggcct
     2941 cggtgccgga gttgaccatg cgcaccatct cgcagctggg caccaggcgc gtgatggttt
     3001 cggccatcgt cacctccagc ggattcggcg tgccgaacga caggccgtcg cgggcggtct
     3061 cgatcaccgc gtccagcacc ttcggatggt tgtggccgac gatcatcggc ccccacgagc
     3121 ccacgtagtc gatgtagcgg ttgccgtccg cgtcgaacag gtacgggccg tccgcacgct
     3181 ggacgaagaa cggttcgccg cccaccgatt tgaacgcgcg caccggcgag ttgacgccgc
     3241 cgggcatcag cgcctgcgcg cgggtgaaga ggtcgtgcga acgttcggaa ttcatgtgtc
     3301 gggttcgtcc ttgaaacagg agaggtaggc gcgcaccgcg gccaccggct cgggggcgtc
     3361 gtacacgccg ctgatcaccg cgagcaggtc ggcaccggcg gcaaccagcg gcggtgcatt
     3421 gtccggggtg atgccgccga tcgccacccg cggcacgccg aggccggcgc tgtcgcgcag
     3481 caagccgatc ccggcacggc gtgcgcccgg cttggtcggc gacgggaaga aggcgccgaa
     3541 cgcgatgtag tcggcgccgg cggcgacggc gtcgcgcgcg cgctgcaggt cgtcgtagca
     3601 ggaggcgccg atgatcgcgt cggcgccaag caatgcccgc gccgcggcaa tcgcgccgtc
     3661 ctgttcgccc aggtgcacgc catcggcacc cagtgcgcgc gccagccgtg ggtcgtcgtt
     3721 gacgatgaag gccacgcccg cctctgcgca ggcatcccgc aatcgcgcac cggccccgcg
     3781 cagcgcgacg gcatccagca ccttgttgcg cagttgcagg caggaggcga atggcagcag
     3841 gggcagggtg cgcgcgagca agcgcccgcc gtcgcgctcg tcgggcgtga tcaggtacaa
     3901 gccgcgttcg ggtccgcgtc cgggcatgtc gcttccgcag ggcggcgcgg ggtgggacaa
     3961 tcgcgccagt ccccgccatt atcgccccat gtccgagcct gccgccgccg atgcccccta
     4021 ccgcacctgg atgtgcgtgg tctgcggctt cgtgtatgac gaggcgctgg gcttgccgga
     4081 ggacggaatc gcgcccggca cccgctggga cgatgtcccg gatacatgga cctgcccgga
     4141 ctgcggcgtc ggcaaggacg acttcgagct gatgcgcgac tgagcgcgca gctcacttcg
     4201 ccagcgggat gccggggccg aaggtgagtt ccgcgccatc ctgcagttgc agccgcgctg
     4261 cttctccggc attgagctcc agcacgtagc gcgccggcgc attgctcggg taggacgggc
     4321 aggcatcgcc cagcgagcat ggcggaatgt cgcgctgctg cgacaccagc ctgcggccgg
     4381 tgtcgaagta caggatgtcg agcgggatct tggtgttctt catccagtac gcctgcggta
     4441 cttcggcatc gtggatgaac agcatgccgc gatcctccgg cagggcgtcg cggaacatca
     4501 gcccgcgcgc gcgctcggcg tcgtcgtcgg cgacctcgat cgcatagcgc tggccggcca
     4561 gctccaccca gggctcgcgc gcactggcgc aggcggaaag cgaagaggcg gcgaacaggg
     4621 caagggcgaa tgcgcgcatg cggggctccg atgaggtggg cgcacattgg cgcgaagcac
     4681 ggattgcgtc aaggcgcaag acttccgctg cgcgcctcga ttcggcatga tgcgcgcccg
     4741 ctccgctgcc ggctgttggc agaggacgga agcaccaaaa gcccacggca aggggtgttg
     4801 acggacgtca aaaaccttgt a
//
LOCUS       DAORUK010000154         4726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_22558_length_4726_cov_20.072575, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000154 DAORUK010000000
VERSION     DAORUK010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22558_length_4726_cov_20.072575"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PK818_09140"
     CDS             complement(<1..377)
                     /locus_tag="PK818_09140"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HQY82932.1"
                     /translation="MTLRRHASYYVAIGILQWLVEYGLMLALSQWFMPVEPANVIGRL
                     AGACLGFWLNGKWTFASDDTHVGRHAAMRFIAMWIALTILNTWIVAFLDDQFGLRTAQ
                     LLKPGADLVSGGIGFLLSRHWVY"
     gene            complement(374..2380)
                     /locus_tag="PK818_09145"
     CDS             complement(374..2380)
                     /locus_tag="PK818_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82933.1"
                     /translation="MPASLRVSRHLPALLLWAGMAACAFGLVAHRLWEALPFPRFFEH
                     LLLALLALAAAWPMQRWRGWQRATALLAVWLAALVVFAGPLPVLAVAVLAATATGLGS
                     LVMRGPVALPLGLALVAGTLGWLLPLPVHHRAAYLACCIGVIAWRRVAIAEAARIAWR
                     QFGDATRAAPRASTAALLLLGLAGTGAWLPTMQYDDVVYHLGLPWQLQQTARYALDPT
                     LQVWALAPWAGDVLHGVVQVLAGVEARGALNGSWLAMAAGAVFALVALLGGDATRRWW
                     AVALLGSLPLSMSLTAGMQTELPAMVLLPVLAWLVLREPAAGPPRGLLAGALLFGALC
                     GLKSMHAAMALPLLAWAGWRHRAHLPWRWLPLAAGIAFAVGGSSYAYAWAIAGNPLLP
                     LLNAWFRSPYFAATDFNDARWQAGLDADVLWDISFDSEHYFESFDGGFGFVLVALAGA
                     WLLALRDPRTRALAIVAGICVLLPLLPLQYARYLQPALALLIPAVVVAYPLLAGTTAA
                     LWTLCVLNLAFATNANWMLRTGALKRAIVSAGADAPVLERYVPERLLAARLREAGNRD
                     GNVLLLPGTGLALAELGQRGRNMLWYSPRWEAEAVRADADASGRAWAALLAGNRIANV
                     ILKPATLAPAQRAGLQRSGALLAGSAGDAQWWRIPGGIPDNGRP"
     gene            2479..3852
                     /gene="glmU"
                     /locus_tag="PK818_09150"
     CDS             2479..3852
                     /gene="glmU"
                     /locus_tag="PK818_09150"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HQY82934.1"
                     /translation="MTVPLHVVILAAGEGKRMKSAVPKVLQAIAGRPMLAHAVDAARA
                     LDPAGVHIVHGHGGEQVRAAFADQPDLYWAEQARQLGTGHAVQQAMPDVPDDARVLVL
                     YGDVPLITPASLERLLQAPGRLAVLAADLQDPTGYGRIVRDAEGRVAAIVEQKDADEV
                     QRRIHLVNTGVIVADATALRRWLEHLRNDNAQGEYYLTDVFAAAADEYAAAEIVVVAD
                     PLETEGANDPWQLAQLERAFQRRQVRALCMDGARVADPARIDIRGRVRVGRDVEIDVD
                     VVFEGEVTLGDGVRIGPFCRLKDVSLAAGTEVRAHCDLDGARTEGAVTIGPFARLRPG
                     TVLADGAHIGNFVETKNATVGVGSKANHLSYLGDAVLGAGVNIGAGTITCNYDGANKS
                     TTTIEDHAFIGSNASLVAPVTVGEGATIAAGSVITKDAPAGKLTVARARQSTLDGWQR
                     PHRKPKA"
     gene            3926..>4726
                     /locus_tag="PK818_09155"
     CDS             3926..>4726
                     /locus_tag="PK818_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HQY82935.1"
                     /translation="MCGIVGAIANRDVVPLLVDGLKRLEYRGYDSAGIAVVAGDAVRR
                     VRRTGRVAEMEAAAATDGLHASLGIGHTRWATHGGVTELNAHPHISHDELALVHNGII
                     ENHEAQRQRLQALGYEFTSQTDTEVVAHLIHHYRAQGDSLMGALQKAVKELEGAYALA
                     VIDKRDPATMVAARMGCPLLVGLGEGENFIASDVSAIISATRRVIFLEEGDTAELTRD
                     GVRVFDAQDALIERDVHVSDVSLASLELGPYRHFMQKEIHEQPRAIGDT"
BASE COUNT          803 a         1702 c         1662 g          559 t
ORIGIN      
        1 ttgtaaaccc aatgccgcga cagcaggaac ccgatcccgc cggacaccag gtcagcgccg
       61 ggcttgagca gttgcgcggt gcgcaggccg aactggtcgt cgaggaaggc gacgatccag
      121 gtgttcagga tggtcagcgc gatccacatc gcgatgaagc gcatcgcggc gtggcggccg
      181 acatgggtgt cgtcgctggc gaacgtccat ttgccgttga gccagaagcc caggcaggca
      241 cccgcgagcc ggccgatcac gttcgcgggt tccaccggca tgaaccactg gctcaacgcc
      301 agcatcagcc cgtattcgac cagccattgc aggatgccga tcgcgacgta atagctggcg
      361 tgccggcgca gggtcatgga cggccattgt ccggaatgcc gccggggatg cgccaccatt
      421 gcgcatcgcc cgcgctgccg gccagcaacg cgccgctgcg ttgcaggccc gcgcgttgcg
      481 ccggggccag ggtggcgggc ttgaggatca cgtttgcgat gcggttgcca gcgagcaacg
      541 ccgcccacgc ccgtccgctg gcgtccgcat cggcgcgaac ggcttcggct tcccagcgcg
      601 gggaatacca gagcatgttg cgaccacgct ggccgagttc ggccagggca aggccggttc
      661 cgggcagcaa cagcacgttg ccgtcgcggt tgcccgcctc gcgcaggcgc gcggccagca
      721 gccgctcggg cacgtaccgc tccagcacgg gtgcatccgc gccggcgctg acgatcgcgc
      781 gcttcaaggc gccggtgcgc agcatccagt tggcgttggt ggcgaacgcg aggttcaaca
      841 cgcacagcgt ccacaacgcg gcagtggtgc cggcaagcag cggataggcg accaccacgg
      901 ccgggatcag caacgccagc gccggctgca ggtagcgcgc gtactgcagc ggcagcaatg
      961 gcagcagcac gcaaatgccg gcgacgatcg caagcgcacg cgtgcgtgga tcgcgcaacg
     1021 ccagcagcca cgcacccgcc agtgccacca gcacgaagcc gaagccgcca tcgaaggatt
     1081 cgaaatagtg ttcgctgtcg aagctgatgt cccacagcac gtcggcgtcc agcccggcct
     1141 gccagcgcgc gtcgttgaaa tcggtggcgg cgaaatacgg tgaacggaac caggcgttga
     1201 gcagcggcag cagcgggttg ccggcgatgg cccaggcata cgcgtagctg gatccgccca
     1261 ccgcgaaggc aatgcccgcc gccagcggaa gccagcgcca cggcaggtgc gcgcggtgcc
     1321 gccaccccgc ccaggccagc agcggcaacg ccatcgccgc atgcatgctc ttcagcccgc
     1381 acaaggcgcc gaacagcagg gcgccggcca gcaagccgcg tggcgggccg gcagccggtt
     1441 cgcgcagcac cagccaggcc agcaccggca gcaggaccat cgccggcaac tcggtctgca
     1501 tgccggcagt caggctcatg ctcagcggca ggctgcccag cagcgccacc gcccaccagc
     1561 gacgcgtggc atcgccaccg agcagcgcca ccagcgcgaa caccgcgccg gccgccatcg
     1621 ccagccacga cccattcagt gcgccacgcg cctcgacacc cgccagcacc tgcaccacgc
     1681 cgtgcagcac gtcaccggcc catggcgcca gcgcccacac ctgcagggtc ggatccagcg
     1741 cgtagcgcgc ggtctgctgc aactgccacg gcagcccgag gtggtagacc acgtcgtcgt
     1801 actgcatggt cggcagccag gcgccggtac cggccagtcc aagcagcaac agcgccgcag
     1861 tcgaggcgcg cggcgcggca cgcgtggcgt ccccgaactg gcgccatgcg atgcgggccg
     1921 cctccgcgat cgccacccgt cgccacgcga tcacgccgat gcagcacgcc aggtacgccg
     1981 cgcggtgatg caccggcagc ggcagcagcc agcccagcgt gccggcgacc agcgccagtc
     2041 ccagcggcag ggcaacgggc ccgcgcatca ccaggctgcc aaggccggtg gcggttgcgg
     2101 caagcacggc caccgccagc accggcaagg gtccggcgaa caccaccagc gccgccagcc
     2161 acacggccag cagcgccgtc gcccgttgcc agccccgcca gcgttgcatc ggccacgccg
     2221 cggccagcgc cagcagggcc agcagcaggt gttcgaagaa gcgcgggaac ggcagcgctt
     2281 cccacagccg gtgcgcgacc aggccgaacg cgcacgccgc catcccggcc cacagcagca
     2341 gcgcgggcag gtggcgggag acgcggaggg acgcgggcat ccggccgatg ctagggcttc
     2401 cgcgggcgtc gtcaatcgct gcatgccggc gaagcggggc tgccgctaga atcgcccggt
     2461 ccgcaaccgg aatagcccat gaccgttccc ctgcacgtcg tcatccttgc cgccggcgaa
     2521 ggcaagcgca tgaaatccgc ggtgccgaag gtgctgcagg cgatcgccgg gcggccgatg
     2581 ctggcgcatg cggtggacgc cgcgcgcgcg ctggacccgg ccggcgtgca catcgtccat
     2641 ggccacggcg gcgagcaggt gcgcgccgcg ttcgccgacc agcccgacct gtactgggcg
     2701 gaacaggcgc ggcagctggg gaccggccac gcggtgcagc aggcgatgcc ggacgtgccc
     2761 gacgatgcgc gcgtgctggt gctgtacggc gacgtgccgc tgatcacccc ggcctcgctg
     2821 gaacgcctgc tgcaggcccc cgggcggctg gcggtgctgg cggcggacct gcaggacccc
     2881 accggctacg gccgcatcgt gcgggatgcc gaggggcggg tggccgcgat cgtcgagcag
     2941 aaggacgcgg acgaggtgca gcgccggatc cacctggtca acaccggggt gatcgtcgcc
     3001 gacgccaccg cgctgcggcg ctggctggag cacctgcgca acgacaacgc gcagggcgag
     3061 tactacctga ccgacgtgtt cgccgcggcc gcggacgagt acgccgcggc ggagatcgtg
     3121 gtggtcgccg atccgctgga aaccgaaggc gcgaacgacc cgtggcagct ggcgcagctc
     3181 gagcgcgcat tccagcggcg gcaggtgcgc gcgctgtgca tggacggcgc gcgcgtcgcc
     3241 gatcccgccc gcatcgacat ccgcggccgg gtgcgggtgg gccgcgacgt cgaaatcgac
     3301 gtcgacgtgg tgttcgaggg cgaggtgacc ctcggcgacg gcgtgcgtat cggcccgttc
     3361 tgccggctga aggatgtttc gctggcggcg ggcaccgagg tgcgcgcgca ttgcgacctc
     3421 gacggcgcgc gcaccgaagg cgcggtgacg atcggcccgt tcgcgcggct gcggcccggc
     3481 accgtgctcg ccgacggcgc ccacatcggc aacttcgtcg agaccaagaa cgcgacggtc
     3541 ggggtcggca gcaaggccaa ccacctgagc tacctcggcg atgcggtgct cggcgcgggc
     3601 gtgaacatcg gcgccggcac catcacctgc aactacgacg gggcgaacaa gtccaccacc
     3661 accatcgagg atcacgcctt catcggttcg aacgcatcgc tggtggcgcc ggtgacggtg
     3721 ggcgagggcg cgaccatcgc ggccgggtcg gtgatcacca aggacgcgcc ggccgggaag
     3781 ctgacggtgg cgcgcgcccg gcagagcacg ctggatggat ggcagcgccc acacaggaag
     3841 ccgaaggcgt agccgtcggg caacgacgtc gcgggcgcgg cgaccggcgg taaactggcg
     3901 cacttgcttc catggatgaa catgcatgtg cggaatcgtc ggcgccatcg ccaaccgtga
     3961 cgtggtcccg ctgctggtcg acggcctcaa gcgcctggaa taccgcggct acgattccgc
     4021 cgggatcgcc gtggtcgcgg gcgatgcggt gcgccgcgtg cgccgcaccg gccgcgtcgc
     4081 cgaaatggag gccgctgcag ccaccgacgg cctgcacgcc agcctcggca tcggccacac
     4141 ccgctgggcc acccacggcg gcgtcaccga gctcaatgcg catccgcaca tcagccacga
     4201 cgagctggcg ctggtccaca acggcatcat cgagaaccac gaggcgcagc ggcagcgcct
     4261 gcaagcgctc ggttacgaat tcacctcgca gaccgacacc gaagtcgtcg cccacctgat
     4321 ccaccactac cgcgcgcagg gcgacagcct gatgggcgcg ctgcagaagg cggtcaagga
     4381 actggaaggc gcctacgcgc tggcggtgat cgacaagcgc gacccggcca ccatggttgc
     4441 cgcgcggatg ggctgcccgc tgctggtcgg ccttggcgag ggcgagaact tcatcgcctc
     4501 cgacgtctcc gcgatcatca gcgccacccg ccgggtgatc ttcctggagg aaggcgacac
     4561 cgccgagctc acccgcgacg gcgtgcgcgt gttcgatgcg caggacgcgc tcatcgagcg
     4621 cgacgtacac gtgtccgacg tctccctggc ctcgctggaa ctgggcccgt accggcactt
     4681 catgcagaag gaaatccacg agcagccgcg cgcgatcggc gacacc
//
LOCUS       DAORUK010000155         4690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_22822_length_4690_cov_18.527724, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000155 DAORUK010000000
VERSION     DAORUK010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22822_length_4690_cov_18.527724"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..735
                     /locus_tag="PK818_09160"
     CDS             178..735
                     /locus_tag="PK818_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014035843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3016 domain-containing protein"
                     /protein_id="HQY82936.1"
                     /translation="MRSKSLVLPLALALAMAGCASTPRSDMLAADAPRALPEAGPVSV
                     RWNDPATFAELRNSHNHYEAARGRWLTDLAEYLRKRAEPRLGSGERLELTIVDVDRAG
                     DYEPWLGPNYQDTRIVRDIYPPRMTVQFRHLGADGRVIAEGERKLTDPAFLIGATPID
                     SSDPLRFEKRMIDSWLRRELVTAAR"
     gene            935..1804
                     /locus_tag="PK818_09165"
     CDS             935..1804
                     /locus_tag="PK818_09165"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HQY82937.1"
                     /translation="MRLRSALLVALLPAIAMSSPATGIGLQAPAPMLASRFDPGLHDP
                     AGCLVSEKLDGVRARWDGGRLLTRGGNVIDVPAWFTSGWPRQPMDGELWTRRGEFQQV
                     SDLVRALRHDDAAWRRVRFMAFDLPADGGGFARRNARLRSLVLAAGADTLGVIAQVPV
                     RDSDALQARLQAVVAAGGEGLMLHRAQARYQAGRSEALLKLKPFDDAEARVVGYRAGK
                     GKYAGMVGALLVVDARGRRFAIGSGLRDAERARPPRIGEQVTFRYQGLTAKGTPRFAR
                     FLRVRRDLAGAPR"
     gene            complement(1824..2909)
                     /locus_tag="PK818_09170"
     CDS             complement(1824..2909)
                     /locus_tag="PK818_09170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQY82938.1"
                     /translation="MSEPVGLPWEGQDTPARRATLTRILVRIAREALQGADLDSLLQG
                     ICECLVAELPVAIASVILLDEANQSFVHEVYDGEWTQSPLAVAGDWPVTRGAAGRCAR
                     LGTAQLITDVDADPDYVAGNVHVRSEYLVPIRHGQRLHGVLNIESTRSDFFDEEARAV
                     FDAVADLVAGAIHFARMADDLQQANRKLEQLSMIDGLTGIANRRRFDQQLCVDWRRMA
                     AEGRPLALLMVDADAFKPLNDAAGHLYGDECLRELARICNGFVDGDNDLVGRFGGEEL
                     VLLLPGRDLAAATAIAEALRIAVEAQAMPHPASPVSAHVTVSVGVAVAVPAAARPPER
                     LIAAADRAMYAAKLQGRNRVCVAGAES"
     gene            3089..4357
                     /locus_tag="PK818_09175"
     CDS             3089..4357
                     /locus_tag="PK818_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQY82939.1"
                     /translation="MLKSLFRQSLFVLRIAAAWGLVVMLVSALYSSIPLIGRLEFPMV
                     LLGMATMALVVGGAFSHLHRVRLIAGRTDADALRNRQRRLVEVPLEAGEAFDLLDAAI
                     RELPGIEQVQGARDSLQVKAKVARPRTYGEHPLRRWNPLLWFGLPRNQLQATVTPGED
                     SGRVTLICEPENPAWSDWFLVDDGTNYENAEAIVRALTRRIGELRRGERASAAQTATQ
                     KELTEARLHLLHAQVEPHFLYNTLASAQLLARSDPARAEAMLGHLIQYLRRSLPNAEG
                     EMSTLGAELERTLAYLEILRIRMGDRLDVQVDVADALRPTPLPAMMLQTLVENAIKHG
                     LEPRSGGGTVWIRARRDGDGVAVTVADNGEGFNTKTSGTGIGLKNVRERLRLRYAGDA
                     NLSVVANFPAGVAATITVPANPPQQGASHV"
     gene            4350..>4690
                     /locus_tag="PK818_09180"
     CDS             4350..>4690
                     /locus_tag="PK818_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY82940.1"
                     /translation="MSDLGKPDPVRTCIVAEDESLLRHALVAELKRAWPALQVVAECE
                     DGASAVEALAEHTPDIAFLDIRMPGLTGLEVAALAADASPRTQIVFVTAYDQYAIDAF
                     ERGAIDYLLKPV"
BASE COUNT          674 a         1720 c         1654 g          642 t
ORIGIN      
        1 gccgcggcca gccgatgacg atgaaggtca agggcacggt cgaggcctgg ttcctggatg
       61 ccaccgaagg aaccggcgcg ccggatcaga acggcggctg accagcgatc cccgcacccc
      121 tcccgcgcgc gcccggcccg gcgcacactg gccttcgcac ccatcgcgga gaccgccatg
      181 cgatccaagt ccctcgtgct gccgttggcg ctcgcgctgg ccatggccgg ctgcgcctcc
      241 accccccgca gcgacatgct ggccgccgat gcaccgcgcg ccctgcccga agcgggcccg
      301 gtgagcgtgc gctggaacga cccggccacc ttcgcggaac tgcgcaacag ccataaccac
      361 tacgaggctg cccgcggtcg ctggctgacc gacctcgcgg aatacctgcg caagcgcgcc
      421 gagccgcggc tgggcagcgg cgaacggctg gaactgacca tcgtcgatgt ggaccgcgcc
      481 ggcgactacg agccctggct cggccccaac taccaggaca cccgcatcgt ccgcgacatc
      541 tacccgccgc ggatgaccgt gcagttccgg catctcggcg ccgatggccg ggtgatcgcc
      601 gaaggcgagc gcaagctgac cgaccccgcc ttcctcatcg gcgccacccc gatcgacagc
      661 agcgatccgc tgcgcttcga gaagcgcatg atcgacagct ggctgcgccg cgagctggtg
      721 accgcagcgc gctgagcgcg ggtggcaggc gggcccggtc gccgccggcg ccagcgccgg
      781 ccggcacctg gaggcctgcc gctgctgcgc aagcgtccgc ggctggcgcg tcggaacccg
      841 cggcaaatcc cgaccacgcg atccgcatct ccccgagtgc gtctcgcgac cgcgttgccg
      901 atgctggcgg cctgcctcca tggaatccgc cccgatgcga ttgcgctccg ccttgctggt
      961 cgccctgctg ccggcgatcg cgatgtcctc gccggccacg ggcatcggcc tgcaggcgcc
     1021 ggcgccgatg ctggccagcc gcttcgatcc cggcctccat gacccggcgg gttgcctggt
     1081 cagcgagaaa ctggatggcg tgcgcgcacg ctgggacggc gggcgcctgt tgacccgcgg
     1141 cggcaacgtc atcgacgtgc cggcgtggtt caccagcggc tggccacgcc agccgatgga
     1201 tggcgagctg tggacgcggc gcggcgaatt ccagcaggtc agcgacctgg tccgcgcgct
     1261 gcgccacgac gacgcggcct ggcgccgcgt ccgcttcatg gccttcgacc tgccggcaga
     1321 cggcggtggc ttcgcccgcc gcaatgcgcg gctgcgctcg ctggtgctgg cggcgggcgc
     1381 ggacacgctc ggggtgatcg cgcaggtgcc ggtgcgcgac agtgacgcac tgcaggcgcg
     1441 gttgcaggcg gttgtcgctg ccggcggcga gggcctgatg ctgcatcgcg cgcaggcgcg
     1501 gtaccaggcc gggcgcagcg aggccctgct caagctgaag ccgttcgacg acgccgaggc
     1561 acgcgtggtc ggctaccgcg cgggcaaggg caagtacgcc gggatggtcg gtgcgttgct
     1621 ggtggtggac gcgcgcggac gccgcttcgc gatcggcagt ggcctgcgcg acgccgagcg
     1681 ggcacggcca ccgcggatcg gcgaacaggt cacgttccgc taccagggcc tgaccgcgaa
     1741 aggcacgccg cgcttcgcgc gcttcctgcg cgtgcgccgc gacctggccg gcgcgccgcg
     1801 ctgagtgtcg cttcgaccgc gattcaggac tcggcgcccg ccacgcagac ccggttgcgg
     1861 ccctgcagct tggccgcgta catggcgcgg tcggcggcgg cgatcaggcg ttccggcggg
     1921 cgcgcggccg cgggcacggc gacggcaacg cccacgctga cggtgacgtg cgcagacacc
     1981 ggcgaggccg gatgcggcat cgcctgcgcc tcgaccgcga tgcgcagggc ttcggcgatg
     2041 gcggtcgcgg cagcgaggtc gcggccgggc aacagcagca ccagctcctc gccgccgaag
     2101 cgccccacca ggtcgttgtc gccatccacg aagccattgc agatgcgcgc gagttcgcgc
     2161 aggcattcgt cgccgtagag gtggccggca gcgtcgttga gcggcttgaa tgcatccgca
     2221 tcgaccatca gcaacgccag cggccggccc tccgccgcca tccgccgcca atccacgcac
     2281 aactgctggt cgaatcggcg gcggttggcg atcccggtca ggccgtcgat catcgacagc
     2341 tgctccagct tgcggttcgc ctgctgcagg tcatccgcca tgcgggcgaa gtggatcgca
     2401 ccggcgacca ggtcggccac cgcatcgaac accgcgcgcg cctcctcgtc gaagaaatcg
     2461 ctgcgggtgc tctcgatgtt gagcacgcca tgcaggcgct ggccgtggcg gatcggcacc
     2521 aggtactcgg agcgcacgtg gacgttgccg gccacgtagt ccgggtcggc atcgacatcc
     2581 gtgatcagct gcgcggtgcc cagccgcgca cagcggccgg cggccccgcg ggtcaccggc
     2641 cagtcgcccg cgaccgccag cggcgactgc gtccactcgc cgtcatagac ctcatggacg
     2701 aaggattggt tggcctcgtc gagcaggatc acgctggcga tcgcgaccgg cagttccgcc
     2761 accaggcatt cgcagatgcc ctgcagcagc gagtccaggt cggcgccctg cagggcctcg
     2821 cgcgcgatcc gcaccaggat ccgggtcagg gtggcacggc gcgccggcgt gtcctgccct
     2881 tcccagggca gccccacggg ctcgctcacc ggatcgtcca tcgcatcgcc atcgcacgca
     2941 cccctgttgg ccgcagtcta cgcctgcgcg ggcgggcttc gtcggcggca gcgccggctt
     3001 cgtcgcgtgc gcgcttccgc tggccgcgcg cctcgctatg ctcgccgcac atcgagttga
     3061 ctcccgtttc cggccaggcc cgccccgcat gctcaagtcc ctcttcaggc aatcgctgtt
     3121 cgtcctgcgc atcgccgccg cgtgggggtt ggtggtgatg ctggtgtccg cgctgtactc
     3181 gagcatcccg ctgatcggcc ggctggaatt cccgatggtg ctgctgggca tggcgacgat
     3241 ggcactggtg gtgggcggcg cgttctcgca cctgcaccgg gtgcgcctga tcgccggccg
     3301 caccgatgcg gatgcgctgc gcaaccgcca gcgtcggctg gtcgaggtcc cgctggaggc
     3361 cggcgaggcg ttcgacctgc tggacgcggc gatccgcgag ttgcccggca tcgaacaggt
     3421 gcagggcgcg cgcgacagcc tgcaggtgaa ggccaaggtg gcgcgcccgc gcacgtacgg
     3481 cgaacacccg ctgcggcgct ggaacccgct gctgtggttc ggcctgccgc gcaaccagct
     3541 gcaggccacg gtgaccccgg gcgaggacag cgggcgggtg accctgatct gcgagccgga
     3601 gaacccggcg tggagcgact ggttcctggt cgacgatggc accaattacg agaacgccga
     3661 ggcgatcgtg cgcgcgctca cccgccgcat cggcgaactg cgccgcggcg aacgcgccag
     3721 cgccgcgcag accgcgaccc agaaggaact caccgaggcc aggctgcacc tgctgcacgc
     3781 gcaggtcgag ccgcacttcc tctacaacac cctggccagc gcgcaactgc tggcgcgcag
     3841 cgatccggcg cgcgccgagg cgatgctcgg ccacctgatc cagtacctgc ggcgttcgct
     3901 gccgaacgcg gaaggcgaga tgtcgaccct gggcgcggag ctggagcgca cgctggcgta
     3961 cctggagatc ctcaggatcc ggatgggcga tcgcctcgac gtgcaggtcg acgtggcgga
     4021 tgcgctgcgc cccaccccgc tgccggcgat gatgctgcag accctggtcg agaacgcgat
     4081 caagcacggg ctggaaccgc gcagcggcgg tggcacggtg tggatccgcg cacgccgcga
     4141 cggggatggg gtggcggtga ccgtggccga caacggcgaa ggcttcaaca ccaagacctc
     4201 gggcaccggc atcggcctga agaacgtgcg cgaacgcctg cgcctgcgct atgccggcga
     4261 cgccaacctg tcggtggtcg ccaacttccc ggccggggtg gccgccacca tcacggttcc
     4321 cgcgaacccg ccgcaacagg gcgccagcca tgtctgacct gggcaaaccc gatccggtcc
     4381 gcacctgcat cgtcgccgag gacgaatccc tgctgcggca tgcactggtg gccgaactca
     4441 agcgcgcgtg gccggcgctg caggtcgtcg ccgaatgcga ggacggcgcc agcgcggtgg
     4501 aagcgctggc cgaacacacc ccggacatcg ccttcctcga catccgcatg cccggcctga
     4561 ccggcctgga agtcgccgcg ctggccgccg acgccagccc gcgcacgcag atcgtgttcg
     4621 tcaccgccta cgaccagtac gcgatcgacg ccttcgagcg cggcgcgatc gactacctgc
     4681 tgaagccggt
//
LOCUS       DAORUK010000156         4670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_22962_length_4670_cov_16.758397, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000156 DAORUK010000000
VERSION     DAORUK010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22962_length_4670_cov_16.758397"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..708
                     /locus_tag="PK818_09185"
     CDS             97..708
                     /locus_tag="PK818_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HQY82941.1"
                     /translation="MKTLCVYCGSNAGSDPAYAALARDLGTRLARDGIALVYGGGNVG
                     LMGIVADAVLAGGGEVIGVIPQQLVDWEVAHKGVTRLEVVDSMHTRKARMFELADGFI
                     ALPGGFGTLDEMFEMLTWRQLGLGRKPCAFLGVNGFWEPLMAMLDTMVRERFLHVEQR
                     SDMWHGTEIEALLEWMRGYVPAQADKWLDEKRRSQLKLTTEDV"
     gene            705..1697
                     /locus_tag="PK818_09190"
     CDS             705..1697
                     /locus_tag="PK818_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HQY82942.1"
                     /translation="MTVPATFRAFRIHDDVDGYRSGIEAVSLDELNAGEVVVKTAYSS
                     VNYKDALAGTGKGKILRRFPLVGGIDIAGHVVASTSPAFREGDAVLVTGSGLSETRDG
                     GYSEYARLDARWVIPLPAGLTLREAMILGTAGFTAALGLLRMTENRQAPDLGPLAVTG
                     ATGGVGSLAIAIFRKAGYEVHAISGKPEHADYLKSLGASEVLGREALTTPRPMDSVRF
                     GGGLDNVGGPMLAALLAQTAPYGNVASCGLAASHELHATVMPFIIRGVSLLGVASAGT
                     ARDIRDEVWRRLASDWKPGNLDAICTREASLDDLPAVFASMLAGGSLGRTVVQL"
     gene            1768..2145
                     /locus_tag="PK818_09195"
     CDS             1768..2145
                     /locus_tag="PK818_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY82943.1"
                     /translation="MARILIVDDSPSQLMGIRRIVEKLGHDALTAEDGAAGVEAAKRE
                     IPDLILMDVVMPNLNGFQATRSITREPSTKHIPVILVTTKDQDTDRMWGLRQGAKAYI
                     TKPFSETELSELIAQYLGGAPAA"
     gene            complement(2222..3139)
                     /locus_tag="PK818_09200"
     CDS             complement(2222..3139)
                     /locus_tag="PK818_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HQY82944.1"
                     /translation="MEFKDYYGILGVEPSAGDAEIKTAYRRLARKFHPDVSKEPGAEE
                     KFKAVNEAFEALRDPAKRKAYDQLRAGGYRPGEEIRPPPGGFGRGPGGQPDFDFDEIF
                     AGGGAGGGFSDFFESMFGRQRGAGGPRGPQAQGRAADGVTRAKLAVPLESVYNGAGVR
                     ISVNGRTLEVRVPKGVRPGQSIRLARQGNGGGDLLLEIEYAAHPQFEVDGRNVIHVLP
                     VAPWEAALGATISVPTLGGPVELKIPADSEAGRKLRLRGRGLPGGAQGADGDQIVELE
                     ILAPKAHDGAQREAYARMRDAFGDDWRRG"
     gene            3239..3718
                     /locus_tag="PK818_09205"
     CDS             3239..3718
                     /locus_tag="PK818_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQY82945.1"
                     /translation="MSIQPGDRIPDAILQCIRDGVQKVDTASLFKGNKLVLFAVPGAF
                     TPTCSERHLPGFVEHFGSFHDKGIGVACMAVNDPFVMQAWGESQHVPEGLQMLSDGNG
                     EFTRKLGLEMDASAFGMGTRSKRFALYAEDGVVRQLFVEAPGEFRVSSAEHVLAELE"
     gene            complement(3786..>4670)
                     /locus_tag="PK818_09210"
     CDS             complement(3786..>4670)
                     /locus_tag="PK818_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HQY82946.1"
                     /translation="TKDEDFIDQLWLVNTHDTLLTFTSSGRVFWLPVHQLPDAGPNAR
                     GRPIINWIPLVEGEKVQAVLPVREYDDAHYVFFATRNGTVKKTPLSEFAFRLARGKIA
                     INLDDGDALIGAALTGGDADIMLFASNGKAVRFDEGAVRSMGRTATGVRGMRLATGEA
                     VVSLIALDGDGDILTASARGYGKRTPVADYPKKGRGTQGVLAIKTSDRNGQLVGAIQL
                     SEQHDVLLISDGGTLVRTPAAQIAQVGRNTQGVTLMRLAADETLQAIERLDASLEGDA
                     EAVAAIPATELPRASGPA"
BASE COUNT          699 a         1657 c         1584 g          730 t
ORIGIN      
        1 gcgccgcgat gtgccctcca gttgacccct cgcaacgcga aaagcacggc gcactccggt
       61 gcgccgtttc cgtttccgcc gggcgataat cgacgcatga aaaccctgtg cgtctattgc
      121 ggttccaacg caggcagcga cccggcctat gccgcgctgg cacgcgacct cggcacccgc
      181 ctcgcccgcg atggcatcgc gctggtctac ggcggcggca acgtcggcct gatgggcatc
      241 gtggccgacg cggtgctggc cggcggcggc gaagtgatcg gcgtgatccc gcagcaactg
      301 gtcgactggg aggtcgccca caagggcgtc acccgcctgg aagtggtcga ttccatgcac
      361 acccgcaagg cgcgcatgtt cgaactggcc gacggcttca tcgccctgcc cggcggcttc
      421 ggcaccctcg acgaaatgtt cgagatgctg acctggcgac agctgggcct gggcaggaag
      481 ccctgcgcct tccttggcgt caacggcttc tgggagccgc tgatggcgat gctggacacc
      541 atggtgcgcg agcgcttcct gcatgtggag cagcgctccg acatgtggca tggcaccgaa
      601 atcgaggcgc tgctggaatg gatgcgcggg tacgtgccgg cgcaggccga caagtggctg
      661 gacgagaagc gccgcagcca gctcaagctc accacggagg atgtatgacc gttccggcga
      721 cgttccgcgc cttccgcatc cacgacgatg tcgacggcta ccgcagcggc atcgaggcgg
      781 tgtcgctgga cgagctcaat gccggcgaag tggtggtcaa gaccgcgtac tcctcggtca
      841 actacaagga cgcgctggcc ggcaccggca agggcaagat cctgcgccgc ttcccgctgg
      901 tcggcggaat cgacattgcc ggccacgtgg tggcctccac cagtcctgcg ttccgcgagg
      961 gcgacgcggt gctggtcacc ggcagcgggc tgtcggagac ccgcgacggc ggctattcgg
     1021 agtacgcccg gctggacgcg cgctgggtca tcccgctgcc cgctggcctc accctgcgcg
     1081 aggcgatgat actgggcacc gccggcttca ccgcagcgct gggcctgctg cggatgaccg
     1141 agaaccggca ggcgcccgac ctggggccgc tggccgtcac cggcgccacc ggcggcgtgg
     1201 gttcgctggc aatcgcgatc ttccgcaagg ccggctacga ggtccacgcg atcagcggca
     1261 agccggagca cgcggactac ctgaagtcgc tcggcgcttc ggaagtgctg ggccgcgagg
     1321 cactgacaac gccccggccg atggacagcg tccgcttcgg cggtggcctg gacaacgtcg
     1381 gcggaccgat gctggccgcg ctgctggcgc agaccgcacc ttacggaaac gtcgccagct
     1441 gcggcctggc cgcctcgcat gagctgcatg ccacggtgat gcccttcatc atccgcggcg
     1501 tctcgctgct gggcgtggcc tcggcgggca ccgcccgcga catccgcgac gaggtctggc
     1561 ggcggctggc cagtgactgg aaaccgggca acctggacgc catctgcacc cgcgaagcct
     1621 ccctggacga cctgccggcg gtgttcgcca gcatgctggc cggcggttcg ctgggccgga
     1681 ccgtggtcca gctctaaaat aacggccaac ccgcttgcgg gcgcaccgcc tgccgctaca
     1741 atcggcctgc caaccacggg gacctacatg gcacgcatcc tgatcgtcga cgactcgcct
     1801 tcccagctca tgggcatccg ccgcatcgtc gaaaaactcg gccacgacgc gctgaccgcc
     1861 gaggatggtg ccgccggcgt ggaagccgcc aagcgcgaga tcccggacct catcctgatg
     1921 gacgtggtga tgccgaacct caacggtttc caggccaccc ggtcgatcac ccgcgagccc
     1981 agcaccaagc acatcccggt gatcctggtg accaccaagg accaggacac cgaccgcatg
     2041 tggggcctgc gccagggcgc gaaggcctac atcaccaagc cgttcagcga aaccgaactg
     2101 tcggagctga tcgcgcagta cctgggcggc gcaccggcgg cctgatcgcc gccgccgctg
     2161 tcgacgccgc gccgccggca tcgctcccca tgccggcctg gtcgccggcg gtcgccttgg
     2221 ctcagccacg cctccaatcg tcgccgaacg catcgcgcat gcgcgcgtag gcctcgcgtt
     2281 gggcgccgtc gtgcgccttc ggcgcgagga tctccagctc cacgatctgg tcgccatccg
     2341 cgccctgcgc gcctcccggc aggccgcgcc cgcgcaagcg cagcttgcgg cccgcctcgg
     2401 aatcggccgg gatcttgagc tccaccgggc cgcccagggt cggcacgctg atcgtcgcgc
     2461 ccagcgccgc ttcccacggc gccactggca gcacatggat gacgttgcgg ccgtcgacct
     2521 cgaactgcgg gtgcgccgcg tattcgattt ccagcagcag gtcgccaccg ccattgccct
     2581 gccgcgccag gcggatcgac tgcccgggcc tgaccccctt cggaacccgc acttcaaggg
     2641 tgcggccgtt gacgctgatg cgcacgccgg cgccgttgta gaccgattcc agcggcaccg
     2701 ccagtttggc gcgggtgaca ccgtccgcgg cgcggccctg cgcctgcggc ccacggggac
     2761 cgcccgcgcc ccgctggcgg ccgaacatgc tctcgaagaa gtcggaaaag ccgccaccgg
     2821 ccccgccgcc agcgaagatc tcgtcgaagt cgaagtccgg ctgcccgccc ggaccgcgac
     2881 cgaacccacc gggcggtggc ctgatctcct cgccgggacg atagccgccc gcgcgcagct
     2941 ggtcgtaggc cttgcgcttg gcaggatcgc gcagtgcctc gaaggcctcg ttgacggcct
     3001 tgaatttctc ctcggcgccg ggttccttgc tgacgtccgg atggaacttg cgcgcaagcc
     3061 ggcgataggc ggtcttgatc tcggcatcgc ccgcactcgg ctcgaccccg aggattccgt
     3121 aatagtcctt gaattccatg ctggtccgtg acgcctcgtt gcctaccctg ccagattatc
     3181 cgatgccggc gcgggcaagg tttagactcc gggttcccca ccgacgagga gcaccacgat
     3241 gtcgatccaa cccggcgacc ggatcccgga cgcgatcctg caatgcatcc gcgatggcgt
     3301 tcagaaggtc gataccgcca gcctgttcaa gggcaacaag ctggtcctgt tcgcggtgcc
     3361 cggcgcgttc accccgacct gttccgaacg gcacctgccg ggcttcgtcg agcacttcgg
     3421 tagcttccac gacaagggca tcggcgtcgc ctgcatggcg gtcaatgatc cgttcgtgat
     3481 gcaggcctgg ggcgagagcc agcacgtgcc ggaggggctg caaatgctgt ccgacggcaa
     3541 tggcgagttc acccgcaagc tgggcctgga gatggatgcc agcgccttcg gcatgggcac
     3601 ccgcagcaag cgcttcgcgc tgtatgccga ggacggggtc gttcgccagc tgttcgtgga
     3661 ggcacccggc gagttccggg tctcgtccgc ggagcacgtg ctggcggaac tggagtaacg
     3721 cggcctcggc cgcggaaacg ggcggccccg cggccgcccg tcgagcgatc gccgctcggt
     3781 ggcggtcagg ccggaccgct tgcccgcggc agctccgtgg ccgggatggc ggcgaccgcc
     3841 tccgcgtcgc cctccagcga ggcatccagg cgttcgatcg cctgcagggt ttcgtccgcc
     3901 gccagccgca tcagggtcac gccctgggtg ttgcggccga cctgggcgat ctgtgccgcg
     3961 ggcgtgcgca ccagggtgcc gccatcggag atcagcagca cgtcgtgctg ttcggagagc
     4021 tggatcgcgc ccaccagctg gccattgcga tcgctggtct tgatcgccag cacgccctgg
     4081 gtgccgcggc ccttcttcgg gtagtcggcg accggggtgc gcttgccgta gccgcgcgcg
     4141 ctggcggtga ggatgtcgcc gtcgccatcg agcgcgatca ggctgaccac tgcctcgccg
     4201 gtggccaggc gcatgccgcg cacgccggtg gcggtgcggc ccatcgagcg taccgcgccc
     4261 tcgtcgaagc gcacggcctt gccgttgctg gcgaacagca tgatgtcggc gtcaccgccg
     4321 gtgagcgcgg caccgatcag cgcgtcgccg tcgtcgaggt tgatcgcgat cttgccgcgg
     4381 gccagccgga acgcgaactc cgacagcggg gtcttcttga ccgtgccgtt gcgggtcgcg
     4441 aagaacacgt aatgcgcatc gtcgtactcg cggaccggca gcaccgcctg caccttctcg
     4501 ccctcgacca gcgggatcca gttgatgatc gggcggccgc gcgcgttggg gccggcgtcc
     4561 gggagctggt gcaccggcag ccagaacacg cggccactgc tggtgaaggt aagcagggtg
     4621 tcgtgggtgt tgaccagcca cagctggtcg atgaaatcct cgtccttggt
//
LOCUS       DAORUK010000157         4602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_23526_length_4602_cov_18.594458, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000157 DAORUK010000000
VERSION     DAORUK010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23526_length_4602_cov_18.594458"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PK818_09215"
     CDS             <1..531
                     /locus_tag="PK818_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HQY82947.1"
                     /translation="GNCIREFVTINRGTGDGGAITRIGDRNWILAYTHVAHDCLIGSD
                     CVFSNNATLAGHVTVGNHVILSGFAGVHQFCRIGDHAFIGMGAFVNGDVPPFVMVAQE
                     KYARARGINAEGLKRRGFDATRIASIKRAYRALYMGDARLDEAKAELGEIADSGSDDV
                     RAMLDFIERGERPLLR"
     gene            647..1789
                     /gene="lpxB"
                     /locus_tag="PK818_09220"
     CDS             647..1789
                     /gene="lpxB"
                     /locus_tag="PK818_09220"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007965611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HQY82948.1"
                     /translation="MSLRIALCAGEASGDQLGAGLIAELRARFPDAHFAGIGGDAMRA
                     AGLDAWFDASELAVMGLAEVLAHLPRLLRLRKAFRQRVLAFQPDVYIGIDAPDFNLGV
                     ERWLKRRGVRCVHYVSPSVWAWRESRAAKIGASADRVLCLFPMEPPIYARHAVDAVFV
                     GHPLADAIPLQPDRAAARAALGAPDAAPVLALLPGSRLGEIERMLPVFIAAASRLRGD
                     MPGLRVLVPAANPQCRAAITRIAGDAPGLRVLDGDAQRAMIASDVVLLASGTAALEAM
                     LCKRPMVVGHRIAGLTYAIVRAFGLLKSAHVSLPNILAGKPLIPELLQDDCTVDGVHA
                     ALLRWFRDGDAVAALQPEFLATHETLRRDASARAADAIASLLPARA"
     gene            1786..2367
                     /gene="rnhB"
                     /locus_tag="PK818_09225"
     CDS             1786..2367
                     /gene="rnhB"
                     /locus_tag="PK818_09225"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQY82949.1"
                     /translation="MTLLVAGVDEAGRGPLAGPVCVAAVILDPARAIDGLDDSKQLSE
                     RKREALYPLILERALGWRIEFVEAAEIDAINILQATLLGMRRAVLGLSPAATHALVDG
                     NRVPDGLPCAATAIIDGDALEPAIMAASVLAKVARDARMRDLHLQFPQYGFDRHKGYP
                     SALHREALRLHGPCPQHRRSFAPVRQALAAAGT"
     gene            2487..>4602
                     /gene="dnaE"
                     /locus_tag="PK818_09230"
     CDS             2487..>4602
                     /gene="dnaE"
                     /locus_tag="PK818_09230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004150958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQY82950.1"
                     /translation="MSARFAHLHLHSEYSLVDSTIRIPELVAACAARGLPAVAVTDQN
                     NLFALVKFYKEAEKVGIKPIAGADVLVAEGAAAPARITLLCRDRAGYLSLSQLLTRAW
                     MEGHRVDGVVARPAWLSANAGLFAIAGPQSEAGQLFASGRHDLAEQALAGLQAGFGER
                     LHLELLRTGRPGEEAFNAFALDVAARRGIPVVASNDVRFLDAAGFDAHEARVCIASGR
                     VLDDARRPHDYSAQQYLKSAEEMAALFAADAADALDNTVALAQRCNLELRLGTYFLPA
                     FPVPSDETLDSWIRSQAREGLAKRLEKAPLAEGRTRGDYEARLDLELGVIVSMGFPGY
                     FLIVADFINWAKQHGIPVGPGRGSGAGSLVAWALGITDLDPLPYDLLFERFLNPERVS
                     MPDFDIDFCMDRRDEVIDYVARKYGRDRVSQIITYGTMAAKAVLRDAGRVLGFPYGFV
                     DGLAKLIPLQPADPMTLEDAIGTGTRAKKEPDRIVGEFRERYETEDDVRDLVDLALQL
                     EDLTRNAGKHAGGVVIAPSPLSDFCPLFAEHDGHGLGRSPVTQFDKDDVESIGLVKFD
                     FLGLRTLTIIDWAVKAINARHAREGRPPLDITALELTDKATYALFARGDSVAVFQFES
                     RGMRELLKRAKPDTFEDIIALAALFRPGPLGSGMDKEWCDRKHGIAEVSYPHPALEQV
                     LAPTYGVIVYQEQVMQIAQVLAG"
BASE COUNT          668 a         1648 c         1603 g          683 t
ORIGIN      
        1 ggcaactgca tccgcgagtt cgtcaccatc aaccgcggca ccggcgacgg cggcgcgatc
       61 acccggatcg gcgaccgcaa ctggatcctt gcctacaccc acgtggcgca cgactgcctg
      121 atcggcagcg attgcgtgtt ctccaacaac gccaccctgg ccggccacgt caccgtcggc
      181 aaccacgtga tcctcagcgg gttcgccggt gtccaccagt tctgccggat cggcgaccat
      241 gccttcatcg gcatgggcgc cttcgtcaac ggcgacgtgc cgccgttcgt gatggtggcg
      301 caggaaaaat acgcgcgcgc gcgcgggatc aacgccgagg gcctgaagcg ccgcgggttc
      361 gatgcaaccc gcatcgccag catcaagcgc gcctaccgcg cgctgtacat gggcgacgcc
      421 aggctggacg aggccaaggc cgaactgggc gagatcgccg attccggcag cgacgacgtg
      481 cgcgcgatgc tggacttcat cgagcgcggc gagcggccgc ttctgcggtg agtaccgggt
      541 tcgtcgggtt cggccatggg gtattcccca agtcgctccc cgatactcgc tttgttggga
      601 aacaccccat ggccgaaccc gccgccgact ttcacgggtg atgtgagtga gcctccgtat
      661 cgccctatgc gccggcgaag cctccggcga ccagctcggc gccggcctga tcgcggaact
      721 gcgcgcgcgc ttccccgacg cacatttcgc cggaatcggt ggcgatgcga tgcgcgccgc
      781 cggcctggac gcatggttcg acgccagcga actggcggtg atgggcctgg ccgaggtgct
      841 ggcgcacctg ccgcgcctgc tgcggctgcg caaggcgttc cgccagcgcg tgcttgcctt
      901 ccagccggac gtgtacatcg gcatcgacgc gccggacttc aacctcggcg tggagcgctg
      961 gctcaagcgg cgcggggtgc gctgcgtgca ctacgtcagc ccgtcggtgt gggcctggcg
     1021 ggagtcgcgc gcggcgaaga tcggcgccag cgccgaccgc gtgctgtgcc tgttcccgat
     1081 ggaaccgccg atctatgccc ggcatgcggt cgacgcggtg ttcgtcggcc acccgctggc
     1141 ggatgcaatc ccgctgcagc cggaccgcgc cgccgcgcgc gcggcgcttg gcgcgccgga
     1201 tgccgcgccg gtcctcgccc tgctgccggg cagccggctc ggcgagatcg agcgcatgct
     1261 gccggtgttc atcgccgccg ccagcaggct gcgtggcgac atgccgggcc tgcgcgtgct
     1321 ggtgcccgcc gccaacccgc agtgccgcgc ggcgatcacg cgcatcgccg gcgatgcacc
     1381 gggcctgcgc gtgctcgacg gcgacgcgca gcgcgcgatg atcgccagcg acgtggtcct
     1441 gctggcctcc ggcaccgccg cgctggaagc gatgctgtgc aagcggccga tggtggtcgg
     1501 ccaccgcatc gccggcctga cctatgccat cgtcagggcg ttcgggctgc tgaagtcggc
     1561 gcatgtcagc ctgccgaaca tccttgccgg caagccactc atcccggaac tgctgcagga
     1621 cgactgcacg gtggacggcg tgcatgcggc gttgttgcgc tggttccgcg atggcgacgc
     1681 ggtggcggca ctgcagccgg aattcctcgc gacccacgaa accctgcgcc gcgatgcatc
     1741 ggcacgcgcc gccgatgcaa tcgcatcgct gctgcccgcg cgcgcatgac cctgctcgtc
     1801 gccggtgtcg acgaagccgg gcgcggtccg ctggcgggac cggtgtgcgt ggccgcggtg
     1861 atcctggatc cggcgcgcgc gatcgatggc ctggacgatt ccaagcagct cagcgagcgc
     1921 aagcgcgagg cgttgtatcc gctgatcctg gagcgcgcgc tggggtggcg catcgaattc
     1981 gtcgaagccg cggagatcga tgccatcaac atcctgcagg cgaccctgct cggcatgcgc
     2041 cgcgccgtgc tggggctgtc gccggcggcc acccatgccc tggtcgacgg caaccgcgtg
     2101 ccggacggcc tgccctgcgc ggccaccgcg atcatcgatg gcgacgcgct ggagccggcg
     2161 atcatggccg cctcggtcct cgccaaggtc gcgcgggatg cgcgcatgcg cgacctgcac
     2221 ctgcaattcc cgcagtacgg cttcgaccgc cacaagggct acccgagcgc cctgcaccgc
     2281 gaggcgctgc gcctgcatgg cccgtgcccg cagcaccggc gcagcttcgc cccggtgcgg
     2341 caggcgctgg cggccgccgg cacctgatcc ccggccgccg cgcagggccc gtcgccgcgc
     2401 gcgcgctgct gtcaaggctt gtccgcgcac ggtggcggcc ctaccctgcg ggttgaccct
     2461 gcgcgtcgcc gcgcccgcgg agcacgatgt ccgcccgttt cgcccacctg cacctgcaca
     2521 gcgagtattc gctggtcgac tcgacgatcc gcatcccgga actcgtcgcc gcctgcgcgg
     2581 cgcgcggctt gccggcggtg gcggtcaccg accagaacaa cctgttcgcg ctggtgaagt
     2641 tctacaagga agccgaaaag gtcggcatca agccgatcgc cggcgccgac gtgctggtcg
     2701 ccgagggcgc cgccgcgccc gccaggatca ccctgctgtg ccgcgaccgc gccggctacc
     2761 tgagcctgtc gcaactgctg acccgggcgt ggatggaagg ccatcgggtc gatggggtgg
     2821 tcgcgcggcc ggcatggctg tcggcgaacg cggggctgtt cgcgatcgcc gggccgcagt
     2881 ccgaggccgg gcagcttttc gcctcgggcc gccacgacct cgccgaacag gcgctggccg
     2941 ggctgcaggc cggcttcggc gaacgcctgc acctggaact gctgcgcacc ggccgccccg
     3001 gcgaggaggc cttcaacgcg ttcgcactgg acgtggcggc gcgccgcggg atcccggtgg
     3061 tcgccagcaa tgacgtgcgc ttcctcgatg cggccggctt cgacgcgcat gaagcccgtg
     3121 tctgcatcgc cagcggacgg gtgctggacg atgcgcgccg cccgcacgac tacagcgccc
     3181 agcagtacct gaaatcggcc gaggaaatgg ccgcgctgtt cgccgccgac gcggcggacg
     3241 cgctcgacaa cacggtggcg ctggcgcagc gctgcaacct ggaattgcgc ctcggcacct
     3301 acttcctgcc ggcattcccg gtgcccagcg acgaaaccct ggacagctgg atccgcagcc
     3361 aggcgcgcga ggggctggcg aagcggttgg agaaggcacc gctcgccgaa ggcaggaccc
     3421 gtggcgacta cgaggcgcgg ctggacctcg aactcggcgt catcgtgtcg atgggcttcc
     3481 ccggctactt cctgatcgtg gccgacttca tcaactgggc caagcagcac gggatcccgg
     3541 tcgggccggg ccgcggctcg ggcgcgggtt cgctggtggc gtgggcgctg ggcatcaccg
     3601 acctcgaccc gctgccgtac gacctgctgt tcgagcgctt cctcaacccg gaacgcgtgt
     3661 cgatgccgga cttcgacatc gacttctgca tggaccgccg cgacgaggtc atcgactacg
     3721 tcgcgcgcaa gtacggccgc gaccgcgtct cccagatcat cacctacggc accatggcgg
     3781 cgaaggcggt gctgcgcgat gccggccgcg tgctcggctt cccgtacggc ttcgtcgacg
     3841 gactggccaa gctgatcccg ctgcagccgg ccgacccgat gacgctggaa gacgcgatcg
     3901 ggaccggcac gcgcgcgaag aaggaaccgg accggatcgt cggcgaattc agggagcgct
     3961 acgagaccga ggacgacgtc cgcgacctcg tcgacctcgc cctgcagctg gaggacctga
     4021 cccgcaatgc cggcaagcac gccggcggcg tggtgatcgc gcccagcccg ctttcggact
     4081 tctgcccgct gttcgccgaa cacgatggcc atggcctcgg ccgcagcccg gtcacccagt
     4141 tcgacaagga cgacgtcgag tcgatcgggc tggtgaagtt cgacttcctc ggcctgcgca
     4201 cgctgacgat catcgactgg gcggtgaagg cgatcaacgc gcggcatgcg cgcgagggcc
     4261 gcccgccact cgacatcacc gcgctggagc tcaccgacaa ggcgacctac gcgctgttcg
     4321 cgcgcggcga ttcggtggcg gtgttccagt tcgaatcgcg cggcatgcgc gaactgctca
     4381 agcgggcgaa gcccgacacc ttcgaggaca tcatcgcgct ggccgcactg ttccgccccg
     4441 gcccgctggg cagcgggatg gacaaggaat ggtgcgaccg caagcacggc atcgccgagg
     4501 tcagctatcc gcacccggcg ctggaacagg tgctggcgcc gacctatggc gtgatcgtgt
     4561 accaggaaca ggtgatgcag atcgcccagg tgctggccgg ct
//
LOCUS       DAORUK010000158         4557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_23863_length_4557_cov_20.612172, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000158 DAORUK010000000
VERSION     DAORUK010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23863_length_4557_cov_20.612172"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PK818_09235"
     CDS             complement(<1..389)
                     /locus_tag="PK818_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQY82951.1"
                     /translation="MAHPVLTALGLSAAESGTAIPGTYVGNGEWSRTADAGVIEPVNP
                     TTGEVLGRVQASSKADYELIVERAQAAFKVWRTTPAPRRGEAIRLCADALRTHKDALG
                     SLVALEMGKSKPEGDGEVQEMIDIGEFA"
     gene            complement(456..532)
                     /locus_tag="PK818_09240"
     tRNA            complement(456..532)
                     /locus_tag="PK818_09240"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(496..498),aa:Arg,seq:cct)
     gene            complement(631..1923)
                     /gene="thrC"
                     /locus_tag="PK818_09245"
     CDS             complement(631..1923)
                     /gene="thrC"
                     /locus_tag="PK818_09245"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQY82952.1"
                     /translation="MRMLSTRGIAPAASISDAIAAGLAPDGGLYMPASLPSFTPADFD
                     GADTLAQVAQRLLRPFFAGDALEGELAAICATAFAVEPPLRTFGEANDYILELFHGAT
                     AAFKDYGASFLAAALAALPRGDRPLTILVATSGDTGAAVGAAFHHKPGMRVAILYPDG
                     RVSPRQAHQLGCFGDNVRALRVDGSFDDCQKLVKTLLSDAAFKQDVPLGSANSISLGR
                     LLPQMAYYAHAALGHWRAGGNPLNFVVPTGNLGNALACFLARSMGLPIGRLVLANNAN
                     DVLARFFEGGDYAPQASRTTLANAMDVGAPSNFERLQWLEPDAARLRAELSAESVSDD
                     EIRATIVDAFQRHGEIVCPHTACAVRVLQRLRARGAVGDWAVVATAHPAKFPEVVEPL
                     IGREVPLPAALAAMLARPSHAQPLAGDARALGDLLRVS"
     gene            complement(1920..2852)
                     /locus_tag="PK818_09250"
     CDS             complement(1920..2852)
                     /locus_tag="PK818_09250"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HQY82953.1"
                     /translation="MNAAPVEVRAFAPGSVGNVGVGFDILGHSIAGIGDVATVRRIDA
                     PAVRIAAIRGTVTDLPLDAQRNTAGMALISLREALDLPFGFELELDKGIPLGSGLGGS
                     AASCVAALVAANALLDAPLAADALYPHALAGEAVASGGRHGDNVGPMLLGGLVLATAE
                     RLTRIEVPAAWHAAVVHPHAVLETRQARAALAGDYALGEFVAQSAQLAQVLLGCERGD
                     VALVRAGLNDLLVEPRRAPLIAGFAAVKEAALAAGAMGASISGAGPSVFAWGESRADA
                     ERAATAMAAAFAGAGFDSDIHLTPVAGPAAAVIA"
     gene            complement(2849..>4557)
                     /gene="thrA"
                     /locus_tag="PK818_09255"
     CDS             complement(2849..>4557)
                     /gene="thrA"
                     /locus_tag="PK818_09255"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017113111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HQY82954.1"
                     /translation="QEACELAYFGAKVIHPQTMTPAIARGLPILMRNTFNPAFAGTRI
                     DAEGGAGGPVKGLTLNTDLALVSLEGAGLIGVPGTAERVFAALHAARISVVMISQGSS
                     EHSICSVVREADADAARNVLLEEFARELSSAQVQGVQVVPGISVLAAVGDGMAGTPGI
                     AAQLFGALARARVNIRAIAQGASERNISVAIAAADQTRALRAAHAAFWLSPQTVALAV
                     IGPGKVGAALLDQLQAALPRLRSEANIDLRLRALATSSKLWLSEHRDSKDWRTRMDEA
                     PIPADLSAIATHLLDTHMPHAAIIDCSASDAVAAHYPQWLAAGIHVITPNKHAGAGPL
                     ERHHAIRAAREKGGARFRYEATVGAGLPVISTLRDLLDTGDELIAIDGILSGTLAWLF
                     NRFDGSVAFSQLVREAHALGYTEPDPRDDLSGTDVARKLTILAREAGMPLSLGDVEVE
                     SLVPESLRAADRDGFMAGLASLDAPMQARLDAARANGCVLRYVARLQRDGTARVALLE
                     LPDDHAFAHLRLTDNCVQFTTRRYCDNPLIVQGPGAGPEVTAAGVFSDLLRVAGSLGA
                     RL"
BASE COUNT          663 a         1599 c         1643 g          652 t
ORIGIN      
        1 gcgaactcgc cgatgtcgat catttcctgc acttcgccgt cgccttccgg cttcgacttg
       61 cccatttcca gcgccaccag cgaacccagc gcatccttgt gggtgcgcag cgcatcggcg
      121 cacaggcgga tcgcctcgcc gcggcgcggt gccggggtgg tgcgccagac cttgaacgcg
      181 gcctgcgcac gctcgacgat caactcgtaa tcggccttgc tggaggcctg cacgcggccc
      241 agcacttcgc cggtggtcgg gttgaccggc tcgatcacgc cggcgtcggc ggtcctggac
      301 cattcgccgt tgccaacgta ggtgccgggg attgccgtgc cggattcggc ggccgacagg
      361 ccgagcgcgg tgaggacggg gtgggccatg gtttcggttc ctgatgaaag cgggagtcgt
      421 agagcggggc ttgccccgct ctacaggagg catgttggtg accccggcgc gattcgaacg
      481 cacgacctgt cccttaggag gggaccgctc tatccagctg agctacgggg ccacagaccc
      541 gcaattatcg cagccgacgg cgccttcggc caatgcgacg gccgtgcttg cgattcgacc
      601 agcaagtctt ggcggattgc ccggctggcc tcaggacacc ctgagcagat cgcccagggc
      661 ccgggcatcg cctgccagcg gctgcgcgtg gctgggccgc gccagcatcg ccgccagcgc
      721 ggctggcagc ggcacttcgc gcccgatcag cggctccacc acttccggga acttcgccgg
      781 atgcgcggtc gccaccaccg cccaatcgcc taccgcgccg cgcgcgcgca ggcgctgcag
      841 gacgcgcacc gcgcacgcgg tatgcgggca gacgatctcg ccgtggcgct ggaacgcatc
      901 gacgatggtc gcgcggatct cgtcgtcgct gacggattcg gcggacagct cggcgcgcag
      961 gcgcgcggca tcgggctcaa gccattgcag gcgctcgaag ttgctgggcg cgccgacgtc
     1021 catcgcgttg gccagggtgg tgcggctggc ctgcggcgcg tagtcgccgc cctcgaagaa
     1081 ccgggccagt acgtcgttgg cgttgttcgc cagcaccagc cggccgatcg gcaggcccat
     1141 gctgcgcgcg aggaaacagg ccagcgcatt gcccaggttg ccggtgggca ccacgaagtt
     1201 cagtgggttg ccgcccgcgc gccagtggcc cagcgcggca tgcgcgtaat aggccatctg
     1261 cggcagcagg cgacccaggc tgatgctgtt ggccgagccc agcgggacgt cctgcttgaa
     1321 cgcggcatcg gacagcaagg tcttcaccag cttctggcag tcatcgaagc tgccgtccac
     1381 ccgcaacgcg cgcacgttgt cgccgaagca gcccaactgg tgcgcctgcc gtggcgagac
     1441 gcggccgtcc gggtacagga tggcgacgcg catcccgggc ttgtggtgga aggcggcgcc
     1501 gaccgcggca ccggtatcgc cggaggtcgc gaccaggatc gtcagcggcc tgtcgccgcg
     1561 cggcagggcc gccagcgcag cggcaaggaa gcttgcgccg tagtccttga acgcggcggt
     1621 ggcgccgtgg aacagttcca ggatgtagtc gttcgcttcg ccgaacgtgc gcagcggggg
     1681 ttcgacggcg aacgcggtgg cgcagatcgc cgcgagttcg ccttccagcg cgtcgcccgc
     1741 gaagaacggc cgcaacaggc gttgcgcaac ctgcgcgagg gtgtcggcgc cgtcgaaatc
     1801 cgcaggtgtg aacgacggca acgacgccgg catgtacagg ccgccatccg gtgccagccc
     1861 ggcggcgatc gcatcgctga tcgatgccgc gggggcgatc ccgcgcgtgc tcagcatcct
     1921 catgcaatca ccgccgcggc cgggccggcg accggggtga ggtggatgtc gctgtcgaag
     1981 ccggcccctg cgaacgcggc ggccatcgcc gtggcggcgc gttcggcatc ggcgcgcgat
     2041 tcgccccacg cgaacacgct gggacccgcg cccgaaatgc tggcacccat cgcacccgca
     2101 gccagcgcgg cctccttcac cgcggcgaag ccggcgatca gcggcgcgcg gcgtggttcc
     2161 accagcaggt cattcaatcc cgcgcgcacc agtgccacgt cgccgcgttc gcagcccagc
     2221 agcacctgcg cgagttgcgc gctctgcgcc acgaattcgc ccagcgcgta atcaccggcc
     2281 agcgcggcgc gtgcctgtcg cgtctccagc accgcatgcg gatgcaccac ggcggcatgc
     2341 cacgcggcgg gcacttcgat ccgggtcagc cgttccgcgg tggccagcac cagcccgccc
     2401 agcagcatcg gcccgacgtt gtcgccatgc ctgccgccgc tggcgaccgc ttcgccggcc
     2461 agtgcgtgcg gatacagggc gtcggcggcg agcggtgcat ccagcagcgc attcgccgcc
     2521 accagcgcgg ccacgcagga cgccgccgag ccgcccaggc cggagccgag cgggatgccc
     2581 ttgtcgagtt ccagctcgaa gccgaacggc aagtccagcg cctcgcgcag tgagatcagg
     2641 gccatgccgg cggtattgcg ctgcgcgtcc agcggcaggt cggtgacggt gccgcggatg
     2701 gcggcgatgc gcacggcggg cgcgtcgatc cggcgcacgg tagccacatc gccgatgccg
     2761 gcgatgctgt ggccgaggat gtcgaaaccg acgccgacat tgcccaccga accgggcgcg
     2821 aatgcgcgga cttccacggg cgcggcgttc acaggcgcgc tcccagcgaa ccggcgacgc
     2881 gcagcaggtc ggagaacacg ccggcggcgg tgacctccgg accggcgccc gggccctgca
     2941 cgatcagcgg gttgtcgcag tagcggcggg tggtgaattg cacgcagttg tcggtcagtc
     3001 gcaggtgggc gaaggcatgg tcgtccggca gttcgagcaa cgcaacccgc gcagtgccgt
     3061 cgcgttgcag gcgggcgaca tagcgcagca cgcagccgtt cgcgcgggct gcatcgagcc
     3121 gcgcctgcat cggcgcgtcc agcgaggcca gtccggccat gaagccgtcg cggtcggcgg
     3181 cgcgcaggga ttcgggcacc aggctttcca cctcgacatc gcccagcgac agcggcatcc
     3241 cggcctcgcg ggcaaggatg gtcagcttgc gtgccacgtc cgtgccggag aggtcgtcgc
     3301 gcgggtcggg ttcggtgtag cccagcgcgt gcgcctcgcg caccagttgc gagaacgcca
     3361 cgctgccgtc gaacctgttg aacagccagg ccagggtgcc ggagaggatg ccgtcgatcg
     3421 cgatcagttc gtcgccggtg tccagcaggt cgcgcagggt ggaaatcacc ggcaggcccg
     3481 cacccacggt ggcttcgtag cggaagcgcg cgccgccttt ctcgcgcgcg gcgcggatcg
     3541 cgtggtgccg ctccagcggc cccgcgcccg cgtgcttgtt gggggtgatc acgtggatgc
     3601 ctgcggccag ccactgcggg tagtgcgccg ctaccgcatc gctggcgctg cagtcgatga
     3661 tcgccgcatg cggcatgtgg gtgtcgagca ggtgggtggc gatggcggag aggtcggcgg
     3721 ggatcggtgc ctcgtccatg cgcgtgcgcc agtccttgct gtcgcggtgt tcgctcagcc
     3781 acagcttgct gctggtggcc agcgcgcgca ggcgcaggtc gatgttggcc tcgctgcgca
     3841 gccgcggcag cgccgcctgc agctggtcga gcagggcggc accgaccttg cccggcccga
     3901 tgacggcgag cgccacggtc tgcggcgaca gccagaacgc ggcgtgcgcg gcgcgcaatg
     3961 cgcgggtctg gtccgcggcg gcgatcgcga ccgagatgtt gcgctcggat gcgccctggg
     4021 cgattgcgcg gatgttcacc cgcgcccgcg ccagcgcacc gaacaactgc gcggcgatgc
     4081 cgggggtgcc ggccatgccg tcgccaaccg cggccagcac gctgatcccg ggcaccacct
     4141 gcacgccctg cacctgcgcg gaggacagtt cgcgcgcgaa ctcctccagc agcacgttgc
     4201 gcgccgcatc ggcatcggct tcgcgcacca ccgagcagat cgaatgctcg gaagaaccct
     4261 gcgagatcat caccaccgag atccgcgccg catgcagggc ggcgaacacg cgttcggcgg
     4321 tgccgggcac gccgatcagg cccgcgcctt ccaggctgac cagggcgagg tcggtgttga
     4381 gcgtcagccc cttcaccggg ccaccggcgc cgccctcggc atcgatgcgc gtgccggcga
     4441 acgcggggtt gaaggtgttg cgcatcagga tcggcaggcc gcgcgcgatg gccggggtca
     4501 tcgtctgcgg atggatcacc ttcgcgccga aataggccag ctcgcaggct tcctggt
//
LOCUS       DAORUK010000159         4537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_24028_length_4537_cov_17.765060, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000159 DAORUK010000000
VERSION     DAORUK010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24028_length_4537_cov_17.765060"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /gene="rpsF"
                     /locus_tag="PK818_09260"
     CDS             complement(<1..406)
                     /gene="rpsF"
                     /locus_tag="PK818_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004090345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HQY82955.1"
                     /translation="MRHYEIVFMVHPDQSEQVPAMIERYKGAIEAGKGTIHRLEDWGR
                     RQLAYPIQNLVKAHYVLMNVEAEQAVLDELVDGFRFNDAVLRHLVMRRDDAVTEQSLI
                     MKNKDEKGDKPERGDRRRRDDESDSAVGTGDTE"
     gene            complement(584..925)
                     /locus_tag="PK818_09265"
     CDS             complement(584..925)
                     /locus_tag="PK818_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HQY82956.1"
                     /translation="MAISLTESARGRIRGYLDADPAAIGLRFGVSRTGCSGWGYKVDM
                     AREAGAGDTVFDADGIRIHVDAASLALVDGTEIDFVKQGLNEQFVFRNPNVAGECGCG
                     ESFTTDAGKAA"
     gene            994..2394
                     /gene="asnS"
                     /locus_tag="PK818_09270"
     CDS             994..2394
                     /gene="asnS"
                     /locus_tag="PK818_09270"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HQY82957.1"
                     /translation="MSMFSVAQALAGRAPEGGQVTVKGWVRTRRDSKAGLSFVNVSDG
                     SCFAPIQVVAPDTLPNYESEVKHLTTGCAVIATGNLVKSQGQGQSFEVQASAIEVVGW
                     VEDPLTYPMQPKQHSLEYLREFAHLRPRTNLFGAVTRIRHCLAQAVHRFFHERQFYWI
                     STPIITSSDAEGAGQMFRVSTLDMANLPRDGKGNVDFSRDFFGKETFLTVSGQLNVEA
                     YCLALSKVYTFGPTFRAENSNTTRHLAEFWMIEPEIAFADLNDDADLAEDFLKYLFRA
                     VLDERGDDLAFIAERVEKTAITRLEGFINAPFERIDYGDAITLLQQSGKKFEFPVEWG
                     LDLQTEHERWLTEEHVGRPVVVMNYPEQIKAFYMRINDDGRTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDARMAQFGLDPAHYGWYRDFRRYGTVPHAGFGLGFERLVVYVCGLAN
                     IRDAIAYPRAPGSAEF"
     gene            2397..2714
                     /locus_tag="PK818_09275"
     CDS             2397..2714
                     /locus_tag="PK818_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011408599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82958.1"
                     /translation="MDADLVLFLALSSFAIAIAGATAFVIFWPLALVHIRDRHPPITA
                     QLGEGAFLKPRALWWLLHGDYRAAQDPYLSGLATPSRVSLLVILGGIAMGGLLWLLAG
                     VLQ"
     gene            2711..3019
                     /locus_tag="PK818_09280"
     CDS             2711..3019
                     /locus_tag="PK818_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82959.1"
                     /translation="MSAPADRWWLATLGRTVVWARLREREAGTAEVFDSDGNTLSYDS
                     TDTARATLMDAEFVEFDGLDEEDALDRGFSLAELAPPQADDDDALRPRMIQQFGMRAR
                     "
     gene            3016..3801
                     /locus_tag="PK818_09285"
     CDS             3016..3801
                     /locus_tag="PK818_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY82960.1"
                     /translation="MKLDLSGKHALVCGGSDGIGRAAAIELAQLGANVTLLARREDVL
                     REAVAALPTHDTQRHGFLVADVSQRQALGDAVSALASDRPVHILVNNTGGPPGGAAHA
                     AGIDAYLDAFNRHLLANHTLLQAVLPGMRAARWGRIVNVISTSVYEPIPNLGVSNTVR
                     GAVASWAKTLSRELGADGITVNNVLPGYTRTRRLDQILAERSAASGKPQAEIASAMLA
                     SVPAGRFADAAEVGSVVAFLCTDAAAYVNGQSLAADGGRMQSI"
     gene            3850..>4537
                     /locus_tag="PK818_09290"
     CDS             3850..>4537
                     /locus_tag="PK818_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQY82961.1"
                     /translation="MQRDPHWIDGKPAMPANGQWLDVFDPATRAVASQVADGDARDVD
                     AAVQAAQAAFPAWSSLPNSARARWLERLADALEARLDAFAHAEAIDAGKPLALARDIE
                     IPRAVGNLRFFAHAATQFSSESHHGEAGLNYTLRPPLGVVGCISPWNLPLYLFTWKIA
                     PALAAGNTVVAKPSEVTPRTATMLAALAAEIGFPDGVLNVVNGRGPTVGEAIVQHPGT
                     KAISFTGSSAV"
BASE COUNT          692 a         1563 c         1543 g          739 t
ORIGIN      
        1 attcggtgtc gccagtgcca accgcactgt cgctctcgtc gtcacggcgg cggcggtcgc
       61 cgcgctccgg cttgtcgccc ttctcgtcct tgttcttcat gatcagggac tgttcggtga
      121 ccgcgtcgtc gcggcgcatc accaggtggc gcagcaccgc gtcgttgaag cggaaaccgt
      181 cgaccagttc gtcgagcacg gcctgctcgg cctccacgtt catcagcacg tagtgggcct
      241 tgaccaggtt ctggatcggg taggccagct ggcggcggcc ccagtcctcc aggcggtgga
      301 tggtgccctt gccggcttcg atcgcgccct tgtagcgctc gatcatggcc ggaacctgct
      361 cgctctggtc gggatggacc atgaacacga tttcgtaatg acgcatggtg gtttccttgt
      421 ggatgtcagc cggctggatg ccggttggga tagccctcgg ccaccggctc cgcgatgcgg
      481 tccggcgcgt tcgggcaagg tctcccgcga acgggagccc aatagtatgg cggatcaatg
      541 acttgggcgc aatccagccg ccacggccgg cgcccgggcc gggtcaggcg gctttgccgg
      601 cgtcggtggt gaagctctcg ccacagccgc actcaccggc cacgttcggg ttgcggaaca
      661 cgaactgttc gttcaggccc tgcttgacga agtcgatctc ggtgccgtcc accagcgcca
      721 ggctggcggc gtcgacgtgg atgcggatcc cgtcggcgtc gaacacggtg tcgccggcgc
      781 ccgcctcgcg cgccatgtcg accttgtagc cccagccgga gcagccggtg cgggacacgc
      841 cgaagcgcag gccgatggcg gccgggtcgg cgtcgaggta gccgcggatg cggccgcggg
      901 cggattcggt gagggagatg gccatgcggg gattgtagct ccggtaaact tgccgctcta
      961 tttcgatgct gcaacgcagg gtttcaagag catatgagca tgttttcggt ggcgcaggcg
     1021 ctggccgggc gcgcgcccga gggtgggcag gtgaccgtga agggctgggt acgcacccgc
     1081 cgcgattcca aggccgggct gagcttcgtc aacgtcagcg acggttcctg cttcgcgccg
     1141 atccaggtgg tcgcgccgga caccctgccc aactacgagt ccgaggtgaa gcacctgacc
     1201 accggctgcg cggtgatcgc caccggcaac ctggtgaagt cgcagggcca gggccagagc
     1261 ttcgaggtgc aggcttccgc catcgaggtc gtcggctggg tggaagatcc gctgacctac
     1321 ccgatgcagc ccaagcagca ctcgctggaa tacctgcgcg aattcgccca cctgcgcccg
     1381 cgcaccaacc tgttcggcgc ggtgacgcgc atccgccatt gcctggcgca ggcggtgcac
     1441 cgcttcttcc acgagcgcca gttctactgg atcagcaccc cgatcatcac cagcagcgac
     1501 gccgaaggcg ccggccagat gttccgcgtc tccaccctgg acatggccaa cctgccgcgc
     1561 gacggcaagg gcaacgtgga tttttcccgc gacttcttcg gcaaggaaac cttcctcacc
     1621 gtgtccggcc agctcaacgt cgaggcctac tgcctggcgc tgagcaaggt ctacaccttc
     1681 ggcccgacct tccgcgccga gaacagcaac accacccggc acctcgccga gttctggatg
     1741 atcgagccgg agatcgcgtt cgccgacctc aacgacgacg ccgacctggc cgaggacttc
     1801 ctgaagtacc tgttccgcgc ggtgctggac gagcgcggcg acgacctcgc gttcatcgcc
     1861 gagcgcgtgg aaaagaccgc gatcacccgg ctggaaggct tcatcaacgc gccgttcgag
     1921 cggatcgact acggcgacgc gatcaccctg ctgcaacagt cgggcaagaa gttcgagttc
     1981 ccggtggaat ggggcctgga cctgcaaacc gagcacgagc gctggctgac cgaggaacac
     2041 gtgggccgcc cggtggtggt gatgaactat ccggagcaga tcaaggcgtt ctacatgcgc
     2101 atcaacgacg acggcaggac cgtggccgcg atggacgtgc tggcaccggg catcggcgag
     2161 atcatcggcg gcagccagcg cgaggaaagg ctggacgtgc tggacgcgcg catggcgcag
     2221 ttcggcctcg acccggccca ttacggctgg tatcgcgact tccgccgcta cggcacggtg
     2281 ccgcacgccg gcttcggcct cgggttcgag cggctggtgg tgtacgtgtg cggcctcgcc
     2341 aacatccgcg acgcgatcgc ctacccgcgc gcacccggca gcgcggagtt ctgagcatgg
     2401 atgcggacct ggtcctgttc ctggcgttga gctcgttcgc gatcgcgatc gccggtgcca
     2461 ccgcattcgt gatcttctgg ccgctggcgc tggtgcatat ccgcgaccgc cacccgccga
     2521 tcaccgcgca actgggcgaa ggcgcgttcc tgaaaccgcg cgcgctgtgg tggctgctgc
     2581 acggggacta ccgcgcggcg caggatccgt acctgtccgg tctggcgacg ccttcgcggg
     2641 tctcgctgct ggtcatcctc ggcgggatcg cgatgggtgg cctgctgtgg ctgctcgccg
     2701 gggtgctgca gtgagcgccc ccgcggaccg ctggtggctg gcaacgctgg ggcgcaccgt
     2761 ggtctgggcg cgcctgcgcg aacgcgaggc cggcaccgcc gaggtgttcg acagcgacgg
     2821 caacaccctc tcctacgaca gcacggacac cgcgcgcgcg accctgatgg atgccgagtt
     2881 cgtcgagttc gacggcctgg acgaggagga tgcgcttgat cgcggattct cgctggccga
     2941 gcttgcgccg ccgcaggccg acgatgacga cgccttgcgc ccgcgcatga tccagcagtt
     3001 cgggatgcgc gccaggtgaa gctggatctc tccggcaagc atgcgctggt ctgcggtggt
     3061 tccgacggca tcggccgcgc cgcggccatc gaactggcgc agctgggggc caacgtcacc
     3121 ctgcttgcac gccgcgagga cgtgttgcgc gaagcggtcg cggcgttgcc cacgcacgac
     3181 acgcagcgcc acgggttcct ggtcgccgac gtttcgcaga ggcaggcgtt gggcgatgcc
     3241 gtctctgcgc tggcatcgga caggccggtg cacatcctgg tcaacaacac cggtggcccg
     3301 cccggcggtg ccgcgcatgc ggccggcatc gatgcctacc tcgatgcctt caacaggcac
     3361 ctgctcgcca accacaccct gctgcaggcg gtgctgcccg gcatgcgcgc cgcgcgctgg
     3421 ggccgcatcg tcaacgtgat ctcgacctcg gtgtacgagc cgatccccaa cctcggcgtg
     3481 tccaataccg tccgcggtgc ggtcgcgagc tgggccaaga cgctttcgcg cgaacttggc
     3541 gcggatggca tcaccgtcaa caacgtgctg cccggctaca cgcgcacccg gcggctggac
     3601 cagatcctgg ccgaacgcag cgccgccagc ggcaagccgc aggcggaaat cgccagcgcg
     3661 atgctggcca gtgtcccggc cgggcgattc gccgatgccg ccgaagtcgg cagcgtggtc
     3721 gccttcctgt gcaccgatgc cgccgcctac gtcaacggcc agtcgctcgc ggcggatggc
     3781 ggccgcatgc agtcgatctg accccgtccc gggacctgcg ctgctgggct ttcgctctag
     3841 actaggcgga tgcaacgcga cccgcactgg atcgatggca agccggcgat gcccgccaac
     3901 gggcaatggc tggacgtgtt cgaccccgcc acccgcgcgg tcgcgtcgca ggtggccgac
     3961 ggcgatgcgc gcgatgtcga tgccgccgtg caggccgcgc aggccgcctt ccccgcctgg
     4021 tcctcgctgc cgaacagcgc gcgtgcgcgc tggttggaac ggctggcgga tgcactggaa
     4081 gcgcgcctcg acgcgttcgc ccatgccgag gccatcgacg ccggcaagcc actggcgctg
     4141 gcccgcgaca tcgagatccc gcgcgcggtc ggcaacctgc gtttcttcgc gcatgcggcc
     4201 acccagtttt ccagcgaatc gcaccacggc gaagccggcc tgaactacac gctgcgcccg
     4261 ccgctgggcg tggtcggctg catcagcccg tggaacctgc cgctgtacct gttcacctgg
     4321 aagatcgcgc cggcgctcgc tgcgggcaac actgttgtag caaaaccctc cgaagtcacc
     4381 ccgcgcacgg cgaccatgct ggcagcgctg gcggcggaga tcggcttccc ggacggcgtg
     4441 ctgaacgtgg tcaatggccg cggcccgacg gtgggtgaag ccatcgtgca gcaccccggc
     4501 accaaggcga tcagtttcac cggcagcagc gcggtcg
//
LOCUS       DAORUK010000160         4517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_24156_length_4517_cov_20.547736, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000160 DAORUK010000000
VERSION     DAORUK010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24156_length_4517_cov_20.547736"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..681
                     /gene="def"
                     /locus_tag="PK818_09295"
     CDS             169..681
                     /gene="def"
                     /locus_tag="PK818_09295"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005911379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HQY82962.1"
                     /translation="MALLPILEFPDPRLRTKAVPVEPAQLAEPSFQQLFDDMFETMYE
                     APGIGLAASQVDVHRRFMVIDVTEDKSRPLVFVNPEISAREGEQVYQEGCLSVPGIYA
                     DVTRSDSITVSALDRHGTPFTIEADGVLAVCIQHEMDHLDGKLFVDYLSPLKREMVRK
                     KLAKARKHAA"
     gene            702..1619
                     /gene="fmt"
                     /locus_tag="PK818_09300"
     CDS             702..1619
                     /gene="fmt"
                     /locus_tag="PK818_09300"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HQY82963.1"
                     /translation="MRIVFAGTPEFAVPCLRAAAGKAEVVAVYTQPDRPAGRGRGLAP
                     SPVKLEAVQRGIPVLQPENFKSALSKQALRALKPDLMVVVAYGLLLPQSVLDIPVHGC
                     WNVHASLLPRWRGAAPIQRAIEAGDTRSGVCLMQMEKGLDTGPVLLAQALDIGANETG
                     GQLHDRLSALGAQVLADGLGLLRASIRLPAYPQPDEGVTYAHKIDKTEARLDWSRPAV
                     ALANRVRAFNPWPMAEAQLAGERVRIHGAVALDEAHAALPGTVLRAGRDGIDVACGTG
                     ALRLRVLQRDGGKAITAADYLNARRDLRA"
     gene            1616..2899
                     /gene="rsmB"
                     /locus_tag="PK818_09305"
     CDS             1616..2899
                     /gene="rsmB"
                     /locus_tag="PK818_09305"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HQY82964.1"
                     /translation="MNSGVATRVSAARVLDAVLHRGSSLKASLAAALPALADPRDRAM
                     VEAIVFAALRQRTRFDAALSQWLPKPLGQRDHEVRALLHAGFAQQALGMPAHAVIAAS
                     VDAARALGRPHQAGLVNALLRRAQRDGLPAAPADGDWPDWLARRVRADWPRQAAEIFA
                     ASAQEAPMWLRVHARATTRDAYLARLHEAGLGADVDAGLADAIRLQASQPVNQLPGFD
                     DGVVSVQDAAAQRVADALAPPAGARVLDACAAPGGKGAHLAERDPSLRITALDVDPRR
                     VRRMQDTFARLRLAIDARAADATHPDRWWDGTPFDAILVDAPCSATGIVRRQPDVLLH
                     RREADIAALAATQSALLDAVWPLLAAGGTLLYATCSILREENAAQVEAFLARTPDAVL
                     EPLAARFGHDTGFGSQRLPGECGMDGFFYARLRKP"
     gene            2923..>4517
                     /locus_tag="PK818_09310"
     CDS             2923..>4517
                     /locus_tag="PK818_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006453166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQY82965.1"
                     /translation="MTRAQRNTRLFWLFALLILGTGLGLRDPWPADEPRFALVAKQMV
                     ESGDWLFPHRGSELYSDKPPLFMWLQAVVLSATGNLRIAFLLPSLLAALGTLWCVVDL
                     GTRLWTRRVGLYAGWALLLALQFTWQAKKAQIDPLAVFWITLANYGLLRHLLRGPDWR
                     MWALGWAAAGLGTITKAVGVLSLLMLLPAAFARWRGWPAVRLHARNPRFWLGPLAFVA
                     ACGVWLLPMLVAALGKQSPAYDAYINDILFRQTVTRYTNAWHHGQPPWYFIEVALTAW
                     LPTVLALPWAIPAWRRRLQRRDARLLLPLGWVLLVLVFFSIPSGKRDMYILPALPMLC
                     LALAPLLPGILRKQGAQRLLLGFVGLLSMAMVGAGLAMLVGEPGFERRFMEGRDAAVA
                     EGLAAMFVATGGVGIGALLWFGRRRAQAALVALLATLWVLYSLVAAPLLNDGSSSRGL
                     MRQVGTAIGPTAQLGLVGWREQQLLMADRPAADFGFKRDPKAQFADALGWQRQASPVP
                     RWLLVEGSVLPACVDTARARDMG"
BASE COUNT          582 a         1553 c         1676 g          706 t
ORIGIN      
        1 cggccatctc cccgctcccc ttggtaaaaa accccctgaa gccgggccac tatagaccac
       61 aagcacgcgc cgctggcaag cggggagcaa ggttgcgcgc ggtacaatcg caaccctgcc
      121 cgcgactgcg gcagcttgcc ccggatatcg tccagcggat tgttcgccat ggccctgctc
      181 ccgatcctcg aattccccga tccgcgcctg cgcacgaagg cggtgccggt cgaacccgca
      241 cagctcgccg aaccgtcgtt ccagcaattg ttcgacgaca tgttcgagac catgtacgaa
      301 gcccccggca tcggccttgc ggccagccag gtcgacgtcc accggcgctt catggtcata
      361 gacgtgaccg aggacaaaag ccggccgctg gtgttcgtga atccggaaat ctccgcccgc
      421 gagggcgagc aggtctacca ggagggctgc ctgtcggtgc ccggcatcta cgccgacgtc
      481 acccgcagcg acagcatcac ggtatcggcg ctggatcgcc atggcacgcc attcacgatc
      541 gaggccgacg gcgtgctggc ggtctgcatc cagcacgaga tggatcacct ggacggcaag
      601 ctgttcgtcg actacctgag cccgctcaag cgcgaaatgg tccgcaagaa gctggcgaag
      661 gcgcgcaagc atgcggccta gcagccgcgc cgcctgaccc gatgcgcatc gtcttcgccg
      721 gcacgccgga gttcgccgtg ccctgcctgc gggcggccgc cggcaaggcc gaggtggtcg
      781 ccgtctacac ccagccggat cgcccggccg ggcgcgggcg cgggctggcc ccgtcgccgg
      841 tgaagctgga ggcggtgcag cgcggaatcc cggtgctgca accggaaaac ttcaaatcag
      901 cgctgtcgaa gcaggcactg cgcgcgctca agcccgacct gatggtggtg gtggcctacg
      961 gcctgctcct gccgcagtcg gtgctggata tccccgtgca tggctgctgg aacgtgcacg
     1021 cttcgctgct gccgcgctgg cgcggcgcgg cgccgatcca gcgcgcgatc gaagccggcg
     1081 acacccgcag cggcgtgtgc ctgatgcaga tggaaaaggg gctggacacc ggcccggtgc
     1141 tgctggcgca ggcgctggac atcggcgcca acgagaccgg cggccagctg cacgatcgcc
     1201 tgtccgcgct cggcgcacag gtgctggccg atggcctggg cctgctgcgc gccagcatcc
     1261 gcctgccggc gtatccgcag ccggacgagg gcgtgaccta cgcgcacaag atcgacaaga
     1321 ccgaggccag gctggactgg tcgcggccgg cggtggcgct ggcgaacagg gtgcgcgcgt
     1381 tcaatccgtg gccgatggcg gaggcgcaac tggccggcga acgcgtgcgc atccacggcg
     1441 cggtggcgct cgatgaagcg catgcggcct tgccggggac cgtgctgcgc gcaggcaggg
     1501 acggcatcga cgtggcctgc ggcactggcg cgctgcggct gcgggtgttg cagcgcgatg
     1561 gcggcaaggc gatcaccgcg gccgactacc tgaacgcacg gcgcgacctg cgcgcatgaa
     1621 cagcggcgtc gccacccgcg tcagcgccgc gcgcgtgctg gatgcggtcc tgcatcgcgg
     1681 gagctctctg aaggcgagcc tggcggcggc gttgccggca ctggctgatc cgcgcgaccg
     1741 ggcgatggtc gaggcgatcg tgttcgccgc cctgcgccag cgcacgcgct tcgatgccgc
     1801 gctgtcgcag tggctgccga aaccactggg ccagcgtgac catgaagtgc gcgccctgct
     1861 gcacgccggg tttgcccagc aggcgctggg gatgccggcg cacgcggtga tcgccgccag
     1921 cgtggatgcc gcgcgcgcac tcgggcgtcc gcaccaggcc gggctggtca acgcgttgtt
     1981 gcggcgcgcg cagcgcgacg ggctgccggc ggccccggcg gatggcgact ggcccgactg
     2041 gctggcccgc cgcgtccgcg ccgactggcc gcgccaggcc gccgagatct tcgccgccag
     2101 cgcgcaggag gcgccgatgt ggctgcgcgt gcatgcacgg gcgaccaccc gcgacgccta
     2161 tcttgcgcgc ctgcatgagg ccggcctcgg ggcggatgtc gatgcggggc tggccgacgc
     2221 gatccggctg caggcctcgc aaccggtgaa ccagctgccg ggcttcgatg atggcgtggt
     2281 ctcggtgcag gacgccgccg cgcagcgggt ggccgatgcg cttgcgccgc cggcaggtgc
     2341 gcgggtgctg gatgcctgcg ccgcccccgg tggcaagggc gcgcacctgg ccgagcgcga
     2401 cccctcgttg cggatcaccg cgctggatgt cgacccgcgc cgggtgcggc gcatgcagga
     2461 caccttcgcg cgcctgaggc tcgccatcga tgcgcgcgcc gcggatgcca cgcacccgga
     2521 tcgctggtgg gacggcacgc cgttcgacgc gatcctggtc gacgcgccat gcagcgccac
     2581 cggcatcgtg cgccggcaac cggacgtgct gctgcaccgg cgtgaagccg acatcgccgc
     2641 gctggccgcc acccaatcgg cgctgctgga tgcggtctgg ccgctgctgg cggcgggcgg
     2701 cacgttgctg tacgccacct gctcgatcct gcgggaggag aacgcggcgc aggtcgaggc
     2761 cttcctcgcg cgcacgccgg atgccgtgct ggaaccgctc gctgcccgct tcggccacga
     2821 caccggcttc ggcagccagc gcctgccggg cgagtgcggg atggacggct tcttctacgc
     2881 gcgactgcgg aagccgtaag cgggcgtcgc tatgatcgcg cgatgacccg agcgcaacgc
     2941 aatacccggc tgttctggct gttcgccctg ctgatcctcg gcaccgggct gggcctgcgc
     3001 gatccatggc cggcggacga accccgcttc gccctcgtcg cgaagcagat ggtggagagt
     3061 ggcgactggc tgttcccgca tcgcggcagc gagctgtatt cggacaagcc accgctgttc
     3121 atgtggctgc aggcggtggt gctgtcggcg accggcaacc tgcggatcgc gttcctgctg
     3181 ccttcgctgc tggcggcgct gggcacgctg tggtgcgtgg tcgacctggg tacccggctg
     3241 tggacgcggc gtgtcgggct gtatgccggc tgggcgctgc tgctggcgct gcagttcacc
     3301 tggcaggcga agaaggcgca gatcgatccg ctggcggtgt tctggatcac cctggccaac
     3361 tacggcctgc tgcgccacct gctgcgcggg cccgactggc ggatgtgggc gctgggctgg
     3421 gcggcggccg ggctgggcac gatcaccaag gcggtcggcg tgctgtcctt gctgatgctg
     3481 ctgccggcgg cgttcgcgcg ctggcgcggt tggccggcgg tgcgcctgca tgcgcgcaat
     3541 ccgcgttttt ggctggggcc gctggcgttc gtcgccgcgt gtggcgtgtg gttgctgccg
     3601 atgctggtgg cggcgctggg caagcagtcg ccggcctacg atgcctacat caacgacatc
     3661 ctgttccggc agacggtgac ccgctacacc aatgcctggc accacggcca gccgccctgg
     3721 tacttcatcg aggtggcatt gaccgcgtgg ctgccgaccg tgctcgcgct gccgtgggcg
     3781 atcccggcct ggaggcgccg cctgcagcgt cgcgacgcgc gcctgctgtt gccgctgggc
     3841 tgggtgctgc tggtgctggt gttcttcagc atcccgtccg gcaagcgcga catgtacatc
     3901 ctgccggcgc tgccgatgct gtgcctggcg ctggcgccgt tgctgccggg gatcctgcgc
     3961 aagcagggcg cgcagcgctt gttgctgggt ttcgtcggac tgctctcgat ggcgatggtg
     4021 ggtgcggggc tggcgatgct ggtcggcgag cccggcttcg agcgccgctt catggagggc
     4081 cgcgacgccg ccgtcgccga ggggctggcc gcgatgttcg tggccaccgg cggggtcggt
     4141 atcggcgcct tgctgtggtt cggccgccgg cgcgcgcagg ccgcgctggt ggcgctgctg
     4201 gccaccctgt gggtgctgta ttcgctggtg gcggcgccgc tgctcaatga cggttcgtcc
     4261 tcgcgcgggc tgatgcggca ggttggcacc gcgatcggcc cgacggcgca gctgggcctg
     4321 gtcggctggc gcgaacagca gttgctgatg gcggatcgcc cggccgctga cttcggcttc
     4381 aagcgcgatc cgaaagcgca attcgccgac gcgctggggt ggcagcggca ggcctccccg
     4441 gttccgcgct ggctactggt cgagggcagc gtgctgccgg cgtgcgtgga caccgcgcgc
     4501 gcgcgcgaca tgggcaa
//
LOCUS       DAORUK010000161         4512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_24196_length_4512_cov_19.087727, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000161 DAORUK010000000
VERSION     DAORUK010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24196_length_4512_cov_19.087727"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..>1922)
                     /locus_tag="PK818_09315"
     CDS             complement(112..>1922)
                     /locus_tag="PK818_09315"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HQY82966.1"
                     /translation="DFPARKVYSPHANQNFPDRVLFGDMHLHTNLSPDAGLLGTSLTA
                     EDAYRAARGRTVTSNTGQPFQLVRPLDFLVVTDHAEYIGLAVMIREANPILLSNPHGK
                     WLWENFTAGGERAMAAFASIVADGTTGNDRLGDTDMGSTIWGDFVKTADRFNEPGVFS
                     AMTGFEWTSMPGGNNIHRVAVFADGADKTGQVLPFSLLDSQNPEDLWKYMENYERNTG
                     GRVLSHLHNGNLSNGTAFSKTRFNGKPVDAAYAEARIRWEPIMEISQIKGDGETHPFL
                     SPDDEFADFERWDVGNLDGSAPKKDEMLQYEYGRSALKVGLELEARLGVNPYQFGFNA
                     SSDAHTGLASTAEDNYFGKMASSEPGPKRFDGEVIRAVDPALRITKSQESAAGLTAVW
                     ARENTRDEIFDSLRRKEIYATTGTRLRVRLFAGWDFAPGDELAPDFAGLGYRKGVPMG
                     GELRGHSAGAAPTFVVSALRDPNWANLDRVQIIKGWLDADGSLKEKIYDVAVSGDRRI
                     GSDGRARDPVGNTVDVAAATFTNTIGAPMLSAYWKDPDFDPAERAFYYVRVLEIPTPR
                     WTTYDARFFGVDLPKNVPATLQDRAYTSAVWYSPQT"
     assembly_gap    1923..2022
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2023..4401)
                     /locus_tag="PK818_09320"
     CDS             complement(<2023..4401)
                     /locus_tag="PK818_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82967.1"
                     /translation="MIDNGIAQLSTSQTVAELELGPGGLLSAPSGIPTVTVNQALRLA
                     GGGTTSVLGSSQMRIALAAGGTGILSANTVMDQGSFLENSGALSLGSASGVALELRVA
                     SSLINHAGGTVTLAGGNSLLPLGPSSELINEAGATFTVNGNALIGEPVPTAGGPRITN
                     QGQFNVNGPGTLTIGGGNGTTLFFQLGALAVSNATFLCNSTASGCQVNDSQGTISTPH
                     QVLLDGGTIDCGGALGSFPLNTTMRLAGSGSLLCRLQQVRGIIAPGTATPGATIAIAG
                     DVQMGAEGGLEFDLGGTAAGQYDRITVGGTFAVGSFDDDGRGRLRLTPAAGYVPALGD
                     AVPVVGYAALQPNGGFNLVESPSALDYATRFDPTALVAYTAPRLTLEPAGVLEGNSGS
                     TTMSVEVRLSQPSSQTVTVRLRPRPGTATSGNPPAGDYTAANPQDLSFSPGTVLRMAQ
                     VTVHGDTQAEASEDMVFDLRRYELAGAAFGNGVRGHLAASGTIIDDDFPPGTRFVLVG
                     KDSASLGIRRYSSSGTYVDTWNTLQTAFGQPNTGMCFAPDGSVLATRFGQAIGGPILF
                     SRNGAYRAVGFGAGAGVQFNSHESCVYDAAGNVYIGQAGFNAQATDAQVPVLKFNPSG
                     TLLDTFVLPTGTRGTDWIELSANQCTLYYTSEDTTVRRYNVCTRSALPDFATGLTAPY
                     CYALRLRPNGELMVACQEAVHRLSAQGANLRTYTRQSIGETDAAGLFALNLDPDGTSF
                     WTAGLNSGSVFRVDIASGAVLASFNSGPGGVGGLAVYDELGETDVLLADGFEG"
BASE COUNT          643 a         1446 c         1609 g          714 t
ORIGIN      
        1 caatgccatt gaacgcgggc gatccggcgg ctgtcgcgat gcgcccgcgc atgaccccgc
       61 gcgggacgcc gcacccgggc agcttcgctg cccgggcgcg gctggaacgg cttatgtctg
      121 cggcgagtac cagacggcgg aggtgtaggc ccgatcctgc agcgtcgccg gcacgttctt
      181 tggcaggtcc acgccgaaga acctggcgtc gtaggtggtc cagcgcggtg tcgggatctc
      241 gagcacccgc acgtagtaga aggctcgttc ggccggatcg aaatccgggt ccttccagta
      301 ggccgagagc atcggcgcgc cgatggtgtt ggtgaaggtg gccgcggcca cgtcgaccgt
      361 gttgccgacc gggtcgcggg cccgcccgtc gctgccgatc ctgcggtcgc cggaaacggc
      421 gacgtcgtag atcttttcct tcagcgagcc gtcggcatcg agccagccct tgatgatctg
      481 cacccggtcg agattggccc agttcggatc gcgcagcgcc gagaccacga aggtgggcgc
      541 ggcgccggcg ctgtgtccac ggagttctcc acccatgggc acgcccttgc gatagcccag
      601 cccggcgaag tcgggcgcca gttcatcgcc cggggcgaag tcccagccgg cgaagaggcg
      661 gacccggagg cgggtgccgg tggtcgcgta gatctccttg cgccgcagcg aatcgaagat
      721 ctcgtcgcgc gtgttctcgc gggcccagac cgccgtgagc ccggcggcgg attcctgcga
      781 cttcgtgatc cgcagggccg gatcgaccgc gcggatgacc tcgccatcga agcgcttggg
      841 gccgggctcg gagctggcca tcttgccgaa gtagttgtcc tcggcggtgg atgccagccc
      901 ggtgtgggcg tcgctcgagg cgttgaaccc gaactggtag ggattgacgc caagcctggc
      961 ttcgagctcc agtccgacct tgagtgccga gcggccgtac tcgtactgca gcatctcgtc
     1021 cttcttcggc gccgagccgt cgaggttgcc gacatcccag cgctcgaaat cggcgaactc
     1081 gtcgtccggc gacaggaagg gatgggtctc gccatcgccc ttgatctggg agatctccat
     1141 gatcggctcc cagcggatcc gcgcctcggc ataggcggcg tccaccggct tgccgttgaa
     1201 gcgggtcttg ctgaacgcgg tcccgttgga gagattgccg ttgtgcaggt gcgacaggac
     1261 ccggccgccg gtattgcgct cgtaattctc catgtacttc cacaggtcct cggggttctg
     1321 gctgtccagc agcgagaacg gcaggacctg gccggtcttg tccgcgccat cggcgaagac
     1381 ggcgacccgg tggatgttgt ttccgcccgg catcgacgtc cattcgaagc cggtcatggc
     1441 cgagaagacg cccggctcgt tgaagcggtc ggcggtcttg acgaagtcgc cccagatggt
     1501 cgaccccatg tcggtgtcgc cgaggcggtc gttgccggtg gtcccatccg cgacgatcga
     1561 cgcgaacgcg gccatggcgc gctcgccgcc ggccgtgaag ttctcccaca gccacttccc
     1621 gtggggattc gacagcagga tcggattcgc ctcgcggatc atcaccgcca ggccgatgta
     1681 ctcggcatgg tccgtgacga cgaggaaatc cagcggcctg acgagctgga agggctggcc
     1741 ggtgttcgag gtcacggtgc ggccgcgcgc ggcccggtac gcatcctccg cggtgagcga
     1801 ggttccgagc aatccggcat ccggcgacag gttggtgtgc aggtgcatgt cgccgaacag
     1861 gacccgatcg gggaagttct gattggcgtg cggcgagtag accttccgcg ccgggaagtc
     1921 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccttcga agccatcggc
     2041 cagcagcacg tcggtctcgc ccagctcgtc gtacaccgcg aggccgccca cgccgcccgg
     2101 gccgctgttg aagctggcca gcaccgcgcc gctcgcgata tcgacacgga acacgctgcc
     2161 gctgttgagg cccgcggtcc agaacgaggt cccgtccgga tcgaggttga gggcgaacag
     2221 gcccgccgca tcggtctcgc cgatgctctg ccgcgtatag gtgcgcaggt tcgcgccctg
     2281 cgcgctgagc cggtgcaccg cctcctggca ggccaccatc agctcgccgt tggggcgcag
     2341 gcgcagcgcg tagcagtacg gcgccgtcag gccggtggcg aagtccggca gcgcgctgcg
     2401 ggtacagacg ttgtagcggc gcacggtggt gtcctcggag gtgtagtaga gcgtgcactg
     2461 gttggccgag agctcgatcc agtcggtgcc gcgggtgccg gtgggcagca cgaaggtatc
     2521 cagcaacgtg ccgctgggat tgaacttgag caccggcacc tgcgcgtcgg tggcttgagc
     2581 gttgaacccg gcctggccga tgtagacatt gccggcggcg tcgtagacgc aggactcgtg
     2641 gctgttgaac tgcacgccgg ccccggcgcc gaagccgact gcgcgatagg cgccgttgcg
     2701 gctgaaaagg atcgggccgc cgatggcctg gccgaagcgc gtggccagca ccgagccatc
     2761 gggcgcgaag cacatgccgg tgttgggctg gccgaaggcg gtctgcaagg tgttccaggt
     2821 atcgacgtag gttccgctgc tgctgtagcg gcggatgccg aggctggcgc tgtccttgcc
     2881 caccagcacg aagcgcgtgc ctggcgggaa gtcgtcgtcg atgatcgttc cgctcgcggc
     2941 caggtggcca cgcacgccgt tgccgaacgc ggcgccggcg agttcgtagc ggcgcaggtc
     3001 gaacaccatg tcctcgctgg cctcggcctg ggtgtcgcca tgcacggtga cctgggccat
     3061 gcgcagcacc gtgcccgggc tgaaactcag gtcctgggga ttggcggcgg tgtagtcgcc
     3121 ggccggcggg ttgccggaag ttgccgtacc cggacgcggg cgcaggcgca cggtgacggt
     3181 ctggctgctc ggctgcgaga gccgcacctc gaccgacatg gtggtgctgc cactgttgcc
     3241 ttcgagcacg ccggccggtt cgagcgtgag gcgcggcgcg gtgtaggcca ccagcgcggt
     3301 cggatcgaag cgcgtggcgt agtccagcgc cgacggcgat tcgaccaggt tgaagccgcc
     3361 gttgggctgc aatgccgcat agcccaccac cggcaccgca tcgcccagcg ccggcacgta
     3421 gcccgcggcg ggcgtcaggc gcaagcggcc ccgaccatcg tcatcgaacg acccgacggc
     3481 gaaggtaccg ccgacggtga tgcgatcgta ttggccggca gcggtgccgc cgaggtcgaa
     3541 ctcgaggccg ccctccgcac ccatctgcac atcgccggcg atggcgatcg tggcgccggg
     3601 tgtcgcggtg cccggcgcga tgatgccgcg cacctgttgc aggcggcaca gcagcgagcc
     3661 gctgccggcg agacgcatcg tggtgttgag cgggaaggag ccgagcgcgc cgccgcaatc
     3721 gatggtgccg ccgtcgagca gcacctggtg cggcgtcgag atcgtgccct ggctgtcgtt
     3781 gacctggcag ccgctggcag tgctgttgca aaggaacgtg gcgttgctca ccgcgagcgc
     3841 gccgagctgg aagaacaacg tggtgccgtt gccgccgccg atcgtgagcg tgcccggacc
     3901 gttgacgttg aactggccct ggttggtgat gcgcggcccg cctgcggtgg gcaccggttc
     3961 gccgatcagc gcgttgccgt tcaccgtgaa ggtggcgcca gcctcgttga tcaactccga
     4021 actgggcccc agcggcagca acgagttgcc gccggcgagc gtgaccgtgc cgccggcgtg
     4081 gttgatcagc gaggaggcga cgcgcagttc cagcgccacg ccgctggcgg aacccaggct
     4141 cagcgcgccg gaattctcga ggaagctgcc ctggtccatc accgtgttcg cgctgaggat
     4201 gccggtgccg cccgcggcca gcgcgatgcg catctgcgaa ctgccgagca ccgaggtggt
     4261 gccgcccccg gcgagccgca gcgcctggtt gaccgtgacg gtcgggatgc cggaaggcgc
     4321 cgagagcagg cccccgggcc cgagctccag ctcggcgacg gtctggctgg tactgagttg
     4381 ggcgatgccg ttgtcgatca acgcgatgtc ggtggcgccc gggctgcggg tcgaggggcc
     4441 cggcatgtcg gcgcaaccgg tccagttcgc gggcgtggtc caggcgccgc tggcggcgtt
     4501 ccactggcag gt
//
LOCUS       DAORUK010000162         4423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_24943_length_4423_cov_21.590888, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000162 DAORUK010000000
VERSION     DAORUK010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24943_length_4423_cov_21.590888"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PK818_09325"
     CDS             complement(<1..742)
                     /locus_tag="PK818_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HQY82968.1"
                     /translation="MSQWYLHDTASNQRLGPMDLDAARAEAARNPALLAWKDGMGDWL
                     PVRNIAELVDGVPGDPAAAPPPPPRNGGKGRADEIDFRIHGQEMQFVEIELDPGESAI
                     AEAGSLMFKDSSVQMDTVFGDGSASGQSGGFMDKLLSAGKRVITGESLFTTLYTHGGS
                     GKAKVAFAAPYPGTVMAMKLDEHGGRIICQKDSFLAGARGVQVGIHLQRKILTGLFGG
                     EGFIMQKLEGDGWVFIHAGGCVVERELAA"
     gene            complement(840..2648)
                     /locus_tag="PK818_09330"
     CDS             complement(840..2648)
                     /locus_tag="PK818_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HQY82969.1"
                     /translation="MRHSLLAATLLTALVAGCKAEAPTTPAASSEAASAAASQADARF
                     AELSKRWLEGWLPLNPVSATQIGDHRFDAELDDVSAAGRQRALDFSKKILAELDAIDA
                     STLSRENQVDASILRNQLRGDIWSTETLQAWAWDPQGYNGLAGGAIYNLMAREFAPLP
                     ERLKSATARMEKLPAMFVQMRANIDVARVPKIHAETVAKQHAGLLSLVDTFITPNADQ
                     LQGADRKRLDAAVAGLRTAVAEHQDWLDKTLVPNAKGDFRIGQKLYDEKLQFSLNSGL
                     TRTDIRQRAEAEIERVRGEMLAVARGVLKDKPGAPALPETPTEAQKQAAIEAALELAY
                     AERPARDKVVEYATRTTRDATDFVRAKDLVTVPDDPVKIILMPEFQRGVAVAYCDSPG
                     PLDKGLDTYYAISPIPDEWSREQVDSFLREYNNRMLHLLSIHEAMPGHYLEGVHSAKH
                     PSTLRAVLRSGPFAEGWAVYTEDTMADAGYLDSDPLFRLVQLKFYLRAVANSILDQGV
                     HVDNWTREQAMDLMVNKTFQQEREAAGKWVRAQLTSAQLATYFVGAQEHFDMRKAVEA
                     KQGAAFNAKQYHDKVLSYGAPPVRFVRQLMLDEPIR"
     gene            2797..3894
                     /locus_tag="PK818_09335"
     CDS             2797..3894
                     /locus_tag="PK818_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82970.1"
                     /translation="MSTTSVTSIVPHPRRHAVPHNTAASDPAPGARAPGGATYRAIFV
                     ALAAALALPANAGLLREGQAVRVIPLSIRDGDKPMVEASIGGRQGVLMIDNGTPDLLF
                     LNRAALALPEGRYLASGFAASGQAVHVQAHPAPRISIAGQPLDLSGDVRSGDFGFTAP
                     GLGTDFLGFIGTPMVADDAFLLDYRHRRLLVLQAGEDGALAVASPGAPDVVVSVPFML
                     LPGEQPTIAASVGTLPFVTDFDTGDSGTLYASAATRDRLRGQGLLEPDGDRWRLGGLA
                     IDGVGFAPTLVRLVIAGSPQDVRKTGHIDQLRLGARFLSANPCLWNFPARTLTFLKPD
                     ATFLDALATAGIDPPRSWASPVSAPAVPGAR"
     gene            3966..>4423
                     /locus_tag="PK818_09340"
     CDS             3966..>4423
                     /locus_tag="PK818_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQY82971.1"
                     /translation="MSRARIALLTLLALVAFAGNSLLCRAALAQTGIDAASFTLVRLL
                     SGAVVLGLLAWLRPGGMRSRGNWLSALALFAYAAAFSFAYLQLTAGAGALLLFGAVQA
                     TMIAHGVWSGERLRPLQLGGLVLACGGLVGLVLPGLSAPPLAAALLMMTAG"
BASE COUNT          590 a         1536 c         1533 g          764 t
ORIGIN      
        1 cggccgccag ctcgcgctcc accacgcagc cgccggcatg gatgaacacc cagccgtcgc
       61 cctcgagctt ctgcatgatg aagccctcgc cgccgaacag gccggtcagg atcttgcgct
      121 gcaggtggat gccgacctgc acgccgcgcg cgccggccag gaagctgtcc ttctggcaga
      181 tgatgcggcc gccgtgttcg tccagcttca tcgccatcac cgtgcccgga tacggcgcgg
      241 cgaacgcgac cttggccttg ccgctgccgc catgcgtgta cagggtggtg aacaggctct
      301 cgccggtgat cacccgcttg cccgcggaca gcagcttgtc catgaagccg ccggactggc
      361 cgctggccga gccgtcgccg aacacggtgt ccatctgcac cgatgaatcc ttgaacatca
      421 gcgagcccgc ttcggcgatg gcggattcgc ccggatccag ctcgatctcg acgaactgca
      481 tttcctggcc gtggatgcgg aagtcgatct cgtcggcgcg gcccttgccg ccgttgcgcg
      541 gcggcggcgg cggtgcggcc gccggatcgc ccggcacgcc atcgaccagt tcggcgatgt
      601 tgcgcactgg cagccagtcg cccatgccgt ccttccacgc cagcagcgcg ggattgcgcg
      661 cggcctcggc gcgcgcggcg tccaggtcca tcgggccgag gcgctggttg ctggcggtgt
      721 cgtgcaggta ccactggctc atggcgaact ccggggttgg ggatgccgcg aagtctatgc
      781 cgcgcgcgat gaccggcaag gtgccggaag tgagggcctg cgcgggcgtg gcgcaggcct
      841 cagcggatcg gctcgtccag catcagctgg cgcacgaagc gcaccggcgg cgcgccgtac
      901 gacagcacct tgtcgtggta ctgcttggcg ttgaacgccg caccctgctt ggcctccacc
      961 gccttgcgca tgtcgaagtg ctcctgcgcg ccgacgaagt aggtggccag ctgcgccgag
     1021 gtcaactgcg cgcgcaccca cttgccggcg gcctcgcgct cctgctggaa ggtcttgttg
     1081 accatcaggt ccattgcctg ctcgcgggtc cagttgtcca cgtgcacgcc ctggtcgagg
     1141 atcgagttgg ccaccgcgcg caggtagaac ttcagctgca ccagccggaa cagcggatcg
     1201 ctgtcgaggt agccggcgtc ggccatcgtg tcctcggtgt acaccgccca gccttccgcg
     1261 aacgggccgg agcgcagcac cgcgcgcagg gtcgacggat gcttggccga atgcacgcct
     1321 tccaggtaat gcccgggcat cgcctcgtgg atcgacagca ggtgcagcat gcggttgttg
     1381 tattcgcgca ggaacgaatc gacctgctcg cggctccact cgtccgggat cggcgagatc
     1441 gcgtagtagg tgtccaggcc cttgtcgagc gggcccggcg aatcgcagta cgccaccgcc
     1501 acgccgcgct ggaactccgg catcaggatg atcttgaccg gatcgtccgg caccgtgacc
     1561 aggtccttgg cgcgcacgaa atcggtggcg tccctggtgg tccgggtggc gtactcgacc
     1621 accttgtcgc gcgccgggcg ctcggcgtag gccagctcca gcgcggcttc gatcgctgcc
     1681 tgcttctgcg cctcggtcgg ggtttccggc aacgccggcg cgcccggctt gtccttgagc
     1741 acgccgcgcg cgaccgcgag catttcgccg cgcacgcgtt cgatctccgc ttccgcgcgc
     1801 tggcggatgt cggtgcgggt cagtccggaa ttcagcgaga actgcagctt ctcgtcgtac
     1861 agcttctggc cgatgcggaa gtcgcccttc gcattcggca ccagggtctt gtccagccag
     1921 tcctggtgtt ccgccactgc cgtgcgcagg ccggcgaccg cggcgtccag gcgcttgcgg
     1981 tccgcgccct gcagctggtc ggcattcggc gtgatgaagg tgtcgaccag gctgagcagt
     2041 ccggcgtgtt gcttggccac ggtctccgcg tggatcttcg gcacccgcgc cacgtcgatg
     2101 ttggcgcgca tctgcacgaa catcgccggc agtttctcca tgcgcgcggt cgccgacttc
     2161 aggcgctccg gcagcggcgc gaactcgcgc gccatcaggt tgtagatcgc gccgccagcg
     2221 aggccgttgt agccctgcgg atcccaggcc caggcctgca gcgtctccgt ggaccagatg
     2281 tcgccgcgca gctggttgcg caggatcgaa gcatccacct ggttctcgcg cgagagcgtg
     2341 ctggcgtcga tggcgtcgag ttcggccagg atcttcttgc tgaaatcgag cgcgcgctgg
     2401 cggccggcgg cgctgacgtc gtcaagctcg gcatcgaagc ggtggtcgcc gatctgggtc
     2461 gcggacaccg gattcagcgg cagccagcct tccagccagc gcttggacaa ttcggcgaag
     2521 cgcgcatccg cctgcgacgc cgcggcgctg gcggcttccg acgaagcggc gggtgtggtc
     2581 ggcgcttcgg ccttgcagcc ggcaaccagg gccgttagca gggtggcggc aagcagggaa
     2641 tggcgcatgc ggcggtctcc gggcaggaaa gaggccaagc ttaggtggaa acccggcagc
     2701 cggcatcgcg gcgcagccgc gcgcgggtcg acgttggccc cgtccctgat gcatcgtggc
     2761 ttcgttcgcc ggtttccgcg ctttcctgga gccttgatgt ccaccacgtc cgtcaccagc
     2821 atcgtcccgc atccgcgaag gcatgcagtc ccccacaaca ccgctgccag cgacccggcc
     2881 cccggagcac gggcaccggg cggggccacc taccgcgcca tcttcgtcgc gctggcggca
     2941 gcactggcct tgcccgcgaa cgccggcctg ctgcgcgaag gacaggccgt gcgcgtgatc
     3001 ccgttatcga tacgcgatgg cgacaagcca atggtcgaag ccagcatcgg cggccggcaa
     3061 ggcgtgctga tgatcgacaa cggcaccccg gacttgctgt tcctcaatcg cgccgcgctc
     3121 gcgctgccgg agggacgcta ccttgccagc gggttcgcgg catcgggcca ggcggtgcac
     3181 gtgcaggcac acccggcgcc gcgcatctcg attgcagggc aaccactgga cctgtccggg
     3241 gacgtgcgca gcggagactt cggcttcacc gcacccggcc tgggcacgga tttcctgggc
     3301 ttcatcggta ccccgatggt ggcggacgat gccttcctgt tggactaccg gcatcgccgg
     3361 ctgctggtgc tgcaagccgg cgaggatggc gcgctggcag tggcatcccc gggcgcgccg
     3421 gatgtcgtgg tgtcggttcc cttcatgctg ttgccgggtg agcagccgac catcgccgca
     3481 tcggtgggga cattgccgtt cgtcaccgac ttcgataccg gcgacagcgg caccctgtac
     3541 gccagcgccg ccacccgcga ccggcttcgc gggcagggcc tgctggagcc ggacggcgac
     3601 cgctggcgcc tgggcgggct ggcgatcgac ggcgtgggct tcgcaccaac cctggtccgc
     3661 ctggtgatcg cgggaagtcc gcaagatgtc cgcaagacgg gccatatcga ccaactccgc
     3721 ctcggtgcgc ggttcctttc ggccaacccc tgcctgtgga acttcccggc caggacgctg
     3781 acattcctca agccggatgc cacgttcctg gacgcattgg caacagccgg gatcgatcca
     3841 ccccgttcct gggcgagccc ggtgtccgcg cctgcggtgc caggcgcgcg ctagcggcgc
     3901 cgattcatgg ccagcctgcc tgccgtacat tgcggcgatc acgtggcgca ttcgggaagg
     3961 gcttgatgtc gcgtgcacgc atcgccttgt tgacgctgct cgcgctggtc gcattcgcag
     4021 gcaactcgct gctgtgccgc gcggcattgg cgcagaccgg gatcgacgcg gccagcttca
     4081 ccctggtgcg cctgctgtcc ggtgcggtgg tgctggggct gctggcctgg ctgcggccgg
     4141 gcggcatgcg ttcacgcggc aactggctgt ccgcgctggc cctgttcgcc tacgccgcgg
     4201 cgttctcgtt cgcctacctg cagctcactg cgggcgcggg cgcgctgctg ctgttcggtg
     4261 ccgtgcaggc caccatgatc gcgcacggcg tgtggtcggg cgagcgcttg cggccgctgc
     4321 aactgggtgg gctggtgctc gcctgtggcg gcctggtcgg cctggtgctg ccgggcctgt
     4381 ccgcgccgcc gctggcggcc gcgctgttga tgatgaccgc cgg
//
LOCUS       DAORUK010000163         4402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_25133_length_4402_cov_25.848171, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000163 DAORUK010000000
VERSION     DAORUK010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25133_length_4402_cov_25.848171"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PK818_09345"
     CDS             complement(<1..538)
                     /locus_tag="PK818_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HQY82972.1"
                     /translation="MLRSGRQLKNHALEAELFRRRALVGFLGVLLALGGLAAWYFRLQ
                     VVQHAEYATRSEANRVRLVPVVPARGLILDRKGRILADNVSAWRLDAVPERAGDGAAL
                     LAKLQRVVAIDAEQGARFLRDYRASRPFKPVPVKLKLSDEEAARFAVDRWRYPGVELV
                     PYLGRVYPHGPLFAHVVGY"
     gene            complement(542..1021)
                     /gene="mreD"
                     /locus_tag="PK818_09350"
     CDS             complement(542..1021)
                     /gene="mreD"
                     /locus_tag="PK818_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012444098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HQY82973.1"
                     /translation="MSRPRNAWVLPVSLLLALLLGLVPLPALLQPVRPYWLALVLAYW
                     VLEEPEKVGLGVAFLLGVVADLAFGGLLGEQAMRLAIFAFILDRFRARLRFFPLSQQA
                     LAIGGLLVNDRVVSAVIHVVLGAPQHPWTFWWAPLLGMLLWAPMHFALDALRLRRRT"
     gene            complement(1018..2055)
                     /gene="mreC"
                     /locus_tag="PK818_09355"
     CDS             complement(1018..2055)
                     /gene="mreC"
                     /locus_tag="PK818_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005990300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HQY82974.1"
                     /translation="MPPYAGPPAARPGDVAGVLRLLVYLVLAVVLMVLDHRGGWLRAA
                     RAQAEVAVQPLWWLAALPSRAGTALREDAVSRSQLMRDNTLLRNALLVSGARNARLQA
                     AAAENARLRGLLASAERGRLDVQLAAILDVDLDPTRQRLVLDAGSRAGVRIGQAVIDA
                     GGLMGQVIATTPTTATVLLVTDPDHAVPAVVARSGVRLIVYGGGRTDALRAADIPLSA
                     DVRTGDVLLSSGMGGRFPPGFTIGTVGVLRADDSRAFLEGQVRPAAQLDRGRDVLLLR
                     GYKPVAAQVPVATAVAPASVPAPSPASAPAPAPASASASASASASASAPASATAPLPP
                     PPPPATTGPAR"
     gene            complement(2066..3109)
                     /locus_tag="PK818_09360"
     CDS             complement(2066..3109)
                     /locus_tag="PK818_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQY82975.1"
                     /translation="MFKFLGRYFSSDLSIDLGTANTLIYVRGQGIVLNEPSVVAVRQD
                     RNGGPRAVAAVGAEAKQMLGRTPGHMTTVRPMKDGVIADFTYTEEMLKHFIRKVHKSR
                     FLRPSPRVLVCVPAGSTQVERRAIKESAEEAGAREVYLIEEPMAAAIGAGMPVTEARG
                     SMVVDIGGGTTEVAVIALNGIVYSQSVRIGGDRFDESIIAYVRRHHGMLIGESTAERI
                     KLELGNAYPQAHVREIEISGRHLAEGVPKIITINSNEVLEALREPLAGIVAAIKLALE
                     QTPPELCADVAERGIVLTGGGALLRDVDRLISEETGLHVQVADDPLTCVARGGGRALE
                     LVDMHGNEFFAPE"
     gene            3322..4257
                     /locus_tag="PK818_09365"
     CDS             3322..4257
                     /locus_tag="PK818_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HQY82976.1"
                     /translation="MAALICGSLAYDTIMVFPDQFKNHILPDKVHILNVSFLVPRMRR
                     EFGGCAGNIAYNLKLLGGDPIPMATVGADFGPYREYFQGLGIELGQVKEIAELFTPQA
                     FITTDLDNNQITAFHPGAMMRSYENHVRDVPDVSIGIVSPDGREGMLQNAAEFAELGI
                     PFIFDPGQAMPLFNGAELRAFIEQAQYVTVNDYESNLLAERTGWSEQEIAAKVKAYIT
                     TRGPQGAQIHADGKTYDIPPAHERRVTDPTGCGDAFRAGLIFGIEKGYDWMTIGRMGN
                     LMGALKVEHPGTQNQRFDYAEFSEQFRQQFGYALG"
BASE COUNT          712 a         1591 c         1511 g          588 t
ORIGIN      
        1 cgtagccgac cacgtgcgcg aacagcggtc cgtgcggata cacccggccg aggtacggca
       61 ccagttccac gccgggatag cgccagcggt cgaccgcgaa gcgcgcggct tcctcgtcgc
      121 tcagtttcag cttgaccggc accggcttga acgggcggct ggcgcggtaa tcgcgcagga
      181 agcgcgcgcc ctgctcggca tcgatcgcaa ccacgcgctg cagcttcgcc agcaacgcgg
      241 cgccgtcgcc ggcgcgctcc ggcaccgcgt ccagccgcca cgcggacacg ttgtcggcga
      301 ggatccggcc cttgcggtcc aggatcagcc cgcgcgccgg caccaccggc accagccgca
      361 cccggttggc ttcggagcgc gtggcgtatt cggcgtgctg caccacctgc aggcggaaat
      421 accaggccgc aagcccgccc agcgcgagca gcacgccgag gaagccgacc agcgcgcgcc
      481 ggcggaacag ctccgcttcc agggcgtggt tcttcagctg gcggccactg cgcagcatgg
      541 ctcaggttcg ccggcgcagg cgcagcgcgt ccagcgcgaa atgcatcggc gcccacagca
      601 gcatgcccag caacggcgcc caccagaacg tccacggatg ctgcggcgcg cccagcacca
      661 cgtggatgac ggcactgacc acgcggtcgt tgaccagcag cccgccgatc gccagcgcct
      721 gctgcgacag cgggaagaac cgcaggcggg cgcggaagcg gtcgaggatg aaggcgaaga
      781 tcgccagccg catcgcctgc tcgcccagca gcccgccgaa cgccaggtcg gcgaccacgc
      841 cgagcaggaa cgccacgcca aggccgacct tctccggttc ctccagcacc cagtacgcca
      901 gcaccagcgc cagccagtag ggccgcaccg gttgcagcag ggccggcaac ggcaccagcc
      961 ccagcagcag ggccagcagc aggctgaccg gcaacaccca ggcattgcgt ggccggctca
     1021 tcgtgccggt cctgtggttg cgggcggcgg cggcggcggg agcggcgcag ttgctgatgc
     1081 aggggcagat gcagatgcag atgcagatgc agatgcagat gcagatgcag gtgctggtgc
     1141 aggtgcagat gcaggtgagg gtgcgggtac ggatgcgggc gccactgcgg ttgccaccgg
     1201 cacctgcgcg gcgaccggct tgtagccgcg cagcagcagc acgtcgcggc cacgatcgag
     1261 ctgcgcggcc ggcctgacct ggccctcgag gaaggcgcgg ctgtcgtccg cgcgcagcac
     1321 gccgacggtg ccgatggtga agccgggcgg gaagcgcccg cccatgccgg aactgagcag
     1381 gacgtcgccg gtacgcacat cggcggacag cgggatgtcg gccgcgcgca gggcatcggt
     1441 gcggccgccg ccgtagacga tcaggcgcac gccgctgcgc gccaccaccg ccggcaccgc
     1501 atggtcgggg tcggtgacca gcagcacggt ggcggtggtc ggggtggtgg cgatcacctg
     1561 ccccatcagg ccgccggcat cgatcaccgc ctggccgatg cgcacgccgg cgcggctgcc
     1621 ggcatccagc accagccgct ggcgggtggg atccaggtcg acgtcgagga tcgccgccag
     1681 ctgcacgtcc aggcggccgc gttcggcgct ggcaagcagc ccgcgcaggc gcgcgttctc
     1741 cgccgccgcc gcctgcagcc gcgcattgcg cgcgcccgac accagcagcg cattgcgcag
     1801 caaggtgttg tcgcgcatca gctggctgcg gctgaccgcg tcctcgcgca gggcggtacc
     1861 cgcgcgcgac ggcagcgcgg ccagccacca cagcggctgc accgcgacct ccgcctgcgc
     1921 gcgcgccgcg cgcaaccagc cgccgcgatg gtcgagcacc atcagcacca ccgccaggac
     1981 caggtagacc agcaggcgca ggacgccggc gacatcgccg gggcgggctg cggggggtcc
     2041 ggcgtagggc ggcacgtggg ggaacttatt ccggcgcgaa gaactcgttg ccgtgcatgt
     2101 ccaccagttc cagcgcgcgg ccgccaccgc gcgccacgca ggtcagcgga tcgtccgcca
     2161 cctgcacgtg caggccggtt tcctcgctga tcaggcggtc gacgtcgcgc agcagggcgc
     2221 cgccgccggt cagcacgatg ccgcgctcgg ccacgtccgc gcacagttcc ggcggggtct
     2281 gctccagcgc cagcttgatc gccgcgacga tcccggccag cggctcgcgc agcgcttcca
     2341 gcacctcgtt ggaattgatg gtgatgatct tgggcacgcc ctcggccagg tggcggccgg
     2401 agatctcgat ttcgcgcacg tgcgcctgcg gataggcgtt gcccagttcc agcttgatcc
     2461 gctcggcggt ggactcgccg atcagcatgc cgtggtggcg gcgcacgtag gcgatgatcg
     2521 actcgtcgaa gcggtcgccg ccgatgcgca cggactgcga gtagacgatg ccgttgagcg
     2581 cgatcaccgc gacctcggtg gtgccgccgc cgatgtccac caccatcgag ccgcgcgcct
     2641 cggtgaccgg catgccggcg ccgatcgccg ccgccatcgg ctcctcgatc aggtacacct
     2701 cgcgcgcgcc ggcttcttcc gccgactcct tgatcgcgcg gcgctccacc tgggtgctgc
     2761 cggccggcac gcacaccagc acgcgcgggc tggggcgcag gaagcgcgac ttgtgcacct
     2821 tgcggatgaa gtgcttgagc atttcctcgg tgtaggtgaa gtcggcgatc acgccgtcct
     2881 tcatcggccg caccgtggtc atgtggccgg gggtgcggcc cagcatctgc ttggcttcgg
     2941 cgccgaccgc ggcgaccgcg cgcggaccgc cgttgcgatc ctggcgcacc gcgaccaccg
     3001 agggttcgtt gagcacgatg ccctggccgc ggacgtagat cagcgtgttg gcggtaccga
     3061 ggtcgatgga gaggtcgctg gagaaatagc gcccgaggaa cttgaacatg cgggaaggct
     3121 gccggaaagt gaatgggcgc gcggggggaa cgcgagacag accgcggctt gcgccgggac
     3181 cgctagccta gcaaccgccc ccagcggcgg caaggagaat cggggccgga atcgcgctgg
     3241 caaccgcgcc ggcgctggcc ggcgggcggc gctgcggcta ccctgtgcga ccctgcgccc
     3301 gaccacctgc gagaccgacc gatggctgcc ctgatctgcg gttccctggc ctacgacacc
     3361 atcatggtgt tccccgacca gttcaagaac cacatcctgc cggacaaggt gcacatcctg
     3421 aacgtgtcct tcctggtgcc gcggatgcgc cgcgagttcg gcggctgcgc cggcaacatc
     3481 gcctacaacc tcaagctgct gggcggcgac ccgatcccga tggccaccgt cggcgccgac
     3541 ttcggcccgt accgcgagta cttccagggc ctcggcatcg aactgggcca ggtcaaggaa
     3601 atcgccgagc tgttcacccc gcaggcgttc atcaccaccg acctcgacaa caaccagatc
     3661 accgccttcc acccgggcgc gatgatgcgc agctacgaga accacgtgcg cgacgtgccg
     3721 gacgtgagca tcggcatcgt cagcccggac ggccgcgaag gcatgctgca gaacgccgcc
     3781 gagttcgccg agctgggcat tcccttcatc ttcgaccccg gccaggcgat gccgctgttc
     3841 aacggcgccg aactgcgcgc gttcatcgag caggcgcagt acgtcaccgt caacgactac
     3901 gaatccaacc tgctggccga acgcaccggc tggagcgagc aggagatcgc cgccaaggtg
     3961 aaggcctaca tcaccacccg cggcccgcag ggtgcgcaga tccacgccga tggcaagacc
     4021 tacgacatcc cgcccgcgca cgaacgccgg gtcaccgatc ccaccggctg cggcgacgcg
     4081 ttccgcgccg gcctgatctt cggcatcgag aagggctacg actggatgac catcggccgc
     4141 atgggcaacc tgatgggcgc gctcaaggtc gaacacccgg gcacccagaa ccagcgcttc
     4201 gactacgccg aattcagcga gcagttccgc cagcagttcg ggtacgcgct gggctgacgc
     4261 cccggcgcgg ctagttccac ttcgccagct tcattgcatc cagcccgccg acctcgaatt
     4321 cggcggtctt cctgcccacg tgcttggtcg ggaattcgat cgacagctgc ttgccggcct
     4381 tggcgagttt ccacagcgcc tt
//
LOCUS       DAORUK010000164         4378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_25342_length_4378_cov_16.255841, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000164 DAORUK010000000
VERSION     DAORUK010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25342_length_4378_cov_16.255841"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="PK818_09370"
     CDS             <1..1479
                     /locus_tag="PK818_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY82977.1"
                     /translation="SFWQIWNMCFGFLGIQFGFALQNANVSRIFQTLGASIDAIPMLW
                     IAAPLTGLLVQPVVGYLSDRTWTRLGRRRPYFLVGALLASAALFAMPASPVLWMAAGL
                     LWVLDASINVSMEPFRAFVGDQLPPRQRTSGFAMQSFFIGVGAVVASALPWMLTAAGV
                     SNTAPAGIVPDSVRYAFWFGGVVLLGAVGWTVLRTREMPPEELRATDSARHAPARAVD
                     AGQARRTGLVWLAAGALVGLGVHRYALDRELYLLAGGLALYGAALLALARGNAADRGM
                     FGTIMRDLYTMPEAMRQLAVVQFFSWFALFAMWIYTTSAVAQVHYGSSDPQSAAYNEG
                     ANWVGVLFAAYNGFAALAAVVIPWMVRRLGLRLAHLVNLFIGGAALLSFFVVRDPQWL
                     LLSMVGVGFAWASILSLPYALLADSLPADKMGIYMGIFNFFIVIPQLVAATVLGFLLK
                     TFFGGAPIHALTLGGLSLALAGLCVLRVRVESEAPRGVAVAP"
     gene            1476..3167
                     /locus_tag="PK818_09375"
     CDS             1476..3167
                     /locus_tag="PK818_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQY82978.1"
                     /translation="MRAGNGAMLALATALLAGCAQTGAPTARPAAVAAPPYYGTTEPF
                     AAEAVYFVVTDRFVNGDESNDQRAQGGAHPSFDIPIAGPDGRSDNIGYLGGDFQGIVD
                     HLDYIKDMGFGAVWITPIIDNPDQAFTGGTPATWGSMWTDQGKTGYHGYWGVNFYRLD
                     EHLPSAGLDFAGFTAALHAKDVKVVLDIVANHGSPAFTMPVAQPQYGQIFDADGELVA
                     DQQNLPPDQLDPGYNPLHRFYNRKSDLAQLSDLREDSPEVMDYLIGAYLQWIGQGADA
                     FRIDTVSLMPGEFWDRFTARIREKHPGYFMFGEVFDYDAAKIAAYTQPGGGSISVLDF
                     PLKQQLARVFGREGGDYALLGPALFLDGGPYRNPYELMTFYDNHDMARLDASDAGFID
                     AHHWLFTARGIPVVYYGSEIGFERGSPELAGNRNYFGTEGIAAARAHPIRAALARIAK
                     VRANSPALQRGLQVNVALAGDLAAFYRVYQREGASQIALVLLNKGDAAADFEVSAMLE
                     PGIWTPALDGDAVTVGTGGSLRASVPAHGAQVYLHAGPVTRSDLAAQLSRQMVAR"
     gene            3285..>4378
                     /locus_tag="PK818_09380"
     CDS             3285..>4378
                     /locus_tag="PK818_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQY82979.1"
                     /translation="MNTTEIFLIAMLIILTAPYLIWRVGRTEYYAPLVVVQIIAGILL
                     GPGVLGAVFPEYYQFVFNPQVIQSLNGIAWWAVMLFVWIAGIELDLHEAWNHRGETVV
                     TAGLALLVPMLLGCAAAIGLLAHGQGWMGPKALAWQFVLGIGMACAVTALPILILFME
                     KIGILRQPLGQRVLRYASFDDVAIWGVLALILMDWTRVGRQGAFLLAFALATLAFRAL
                     MRRLPERDRWYVGLIWLAACGFAADWAGLHFMVGAFLSGAVIDGSWFDREQMDRLRHN
                     VLLVVMPVFFLSTGLRTNWTLGGATVFIAAGVLLFAAVAGKLAGVHLAGRILRWERGE
                     ASIIGWLLQTKALIMIIFANILLDKAIITS"
BASE COUNT          579 a         1554 c         1493 g          752 t
ORIGIN      
        1 tcgttctggc agatctggaa catgtgcttc ggcttcctcg gcatccagtt cggcttcgcg
       61 ctgcagaacg ccaatgtcag ccgcatcttc cagaccctgg gcgcgtcgat cgacgccatc
      121 ccgatgctct ggatcgcggc tccattgacc gggctgctgg tgcagccggt ggtgggatac
      181 ctctcggacc gcacctggac ccgcctgggc cggcgccggc cgtacttcct ggtcggggca
      241 ctgctggctt cggccgcgct gttcgcgatg ccggcatcgc cggtgctgtg gatggcggcg
      301 gggctcctgt gggtgctcga cgcctcgatc aacgtctcga tggagccctt ccgcgccttc
      361 gtcggcgacc agctgccccc gcgccagcgc accagcggct tcgccatgca gagcttcttc
      421 atcggcgtcg gcgcggtggt cgccagcgcc ttgccctgga tgctgaccgc ggccggggta
      481 tcgaacacgg cgccggccgg gatcgtgccg gattcggtcc gctacgcgtt ctggttcggc
      541 ggcgtggtcc tgctgggcgc ggtcggctgg accgtgctgc gcacgcgcga gatgccgccc
      601 gaggaactgc gcgccaccga tagcgcgcgc cacgcgccgg cgcgtgccgt cgatgcaggc
      661 caggcccgcc ggaccggcct ggtctggctc gcggccggcg ccctggtggg actgggcgtc
      721 catcgctacg cgctggatcg cgagttgtac ctgctggccg gcggcctcgc cctgtacggc
      781 gcggcgctgc tcgcgctggc gcgcggaaac gccgccgacc gcggcatgtt cggcacgatc
      841 atgcgcgacc tctacaccat gcccgaggcg atgcggcaac tggcggtggt gcagttcttc
      901 tcgtggtttg cgctgttcgc gatgtggatc tacaccacct cggccgtggc ccaggtgcac
      961 tatggctcca gcgacccgca gtcggcggcg tacaacgaag gcgccaactg ggtcggcgtg
     1021 ctgttcgcgg cctacaacgg cttcgccgcg ctcgcggccg tggtgatccc gtggatggtg
     1081 cggcgcctgg gcctgcggct ggcgcacctg gtgaacctgt tcatcggcgg tgcggccctg
     1141 ctctcgttct tcgtcgtccg cgacccgcaa tggctgctgc tgtcgatggt cggggtcgga
     1201 ttcgcctggg cctcgatcct ttcgctgccc tatgcgctgc tggccgacag cctgccggcg
     1261 gacaagatgg gcatctacat gggcatcttc aatttcttca tcgtcatccc gcagctggtc
     1321 gcggccaccg tgctgggctt cctgctcaag accttcttcg ggggggcgcc gatccacgcc
     1381 ctgaccctgg gcggactgag cctggcgctg gcaggcctgt gcgtgctgcg ggtgcgggtc
     1441 gagtccgagg cgccgcgcgg cgtggcggtg gcgccatgag ggccggcaat ggcgcgatgc
     1501 tggcgctggc gacggcgctg ctcgccggct gcgcgcaaac cggggcgccg accgcgcgcc
     1561 ccgccgcggt ggccgcgccg ccgtactacg gcaccaccga acccttcgcg gccgaggccg
     1621 tgtacttcgt cgtcaccgac cgcttcgtca acggcgacga gtccaacgac cagcgcgcgc
     1681 agggcggggc gcatcccagt ttcgacatcc cgatcgcggg gccggacggg cgcagcgaca
     1741 acatcggcta cctcggtggc gacttccagg gcatcgtcga ccacctcgac tacatcaagg
     1801 acatgggctt tggcgcggtg tggatcacgc cgatcatcga caatcccgac caggccttca
     1861 ccggcggcac gccagccacg tggggcagca tgtggaccga ccagggcaag accggctacc
     1921 acggttactg gggcgtgaac ttctaccggc tcgacgagca cctgcccagt gcggggctgg
     1981 atttcgccgg cttcaccgcc gcgctgcacg ccaaggacgt caaggtcgtg ctcgacatcg
     2041 tcgccaacca cggctcgccg gccttcacca tgccggtggc gcagccgcag tacggccaga
     2101 tcttcgatgc cgacggcgag ctggtcgccg accagcagaa cctgccgccc gaccagctcg
     2161 atccgggcta caacccgctg catcgtttct acaaccgcaa gagcgacctc gcgcaactgt
     2221 cggacctgcg cgaggactcg cccgaggtga tggattacct gatcggcgcc tacctgcaat
     2281 ggatcgggca gggcgcggac gcgttccgca tcgacaccgt gagcctgatg ccgggcgagt
     2341 tctgggatcg attcaccgcg cgcatccgcg aaaaacaccc cggctacttc atgttcggcg
     2401 aggtcttcga ctacgacgcc gcgaagatcg cggcgtacac gcagcccggc ggtggttcga
     2461 tctcggtgct cgacttcccg ctcaagcagc agctcgcccg ggtcttcggg cgtgaaggcg
     2521 gcgactatgc cctgctgggc ccggcgctgt tcctcgacgg cggcccgtac cgcaatccct
     2581 acgagctcat gaccttctac gacaaccacg acatggcgcg gctcgacgcc agcgacgccg
     2641 gcttcatcga cgcgcaccat tggctgttca cggcgcgcgg catcccggtc gtgtactacg
     2701 gctcggagat cggcttcgag cgcggcagcc ccgagctggc cggcaaccgc aactacttcg
     2761 ggacggaagg catcgcggcg gcgcgcgcgc atccgatccg cgcggcgctg gcccggatcg
     2821 ccaaggtccg cgccaactcg ccggcgctgc agcgcggcct ccaggtcaac gtggcgctgg
     2881 ccggggatct cgcggccttc taccgcgtct accagcgcga gggcgcgagc cagatcgcac
     2941 tggtgctgct caacaagggc gatgcggcgg ccgacttcga ggtctcggca atgctcgagc
     3001 ccggcatctg gaccccggcg ctggacggcg atgcagtgac ggtgggcaca ggcggcagcc
     3061 tgcgcgccag cgtcccggcg cacggcgcgc aggtctacct gcacgcgggg ccggtcacgc
     3121 gcagcgacct ggccgcgcag ctgtcgcgcc agatggtcgc gcgctgatcc ggttccgcgt
     3181 tctatccttg caccgccgcg gacgcgcgcc ggcacggccc atccgtggcc gcgcgcaagt
     3241 ccttcgcgcg cgccagcccc cagggagttc cgatgccagc ggcgatgaac accaccgaga
     3301 tcttcctgat cgcgatgctg atcatcctga ccgcgccgta cctgatctgg cgcgtcggcc
     3361 gcaccgagta ctacgcgccg ctggtcgtgg tgcagatcat cgccgggatc ctgctcggtc
     3421 ccggggtcct cggcgcggtg ttccccgagt actaccagtt cgtgttcaac ccgcaggtga
     3481 tccagtccct caatggcatc gcctggtggg cggtgatgct gttcgtctgg atcgccggga
     3541 tcgagctcga cctgcacgag gcctggaacc accgcggcga aaccgtggtc acggccggac
     3601 tggccttgct ggtgccgatg ctgctgggtt gcgcggccgc gatcggcctg ctcgcccacg
     3661 gccagggctg gatggggccc aaggccctgg cctggcagtt cgtgctcggc atcggcatgg
     3721 cctgcgcggt caccgccctg ccgatcctga tcctgttcat ggagaagatc gggatcctgc
     3781 gccagccgct gggccagcgc gtgctgcgct acgccagctt cgacgatgtc gcgatctggg
     3841 gcgtgctcgc cctgatcctg atggactgga cccgcgtcgg ccgccagggc gccttcctgc
     3901 tggcctttgc gctggccacg ctggcctttc gcgcgctgat gcgccgcctg cccgagcgcg
     3961 accgctggta cgtgggcctg atctggctcg ccgcctgcgg cttcgccgcg gattgggccg
     4021 gactgcactt catggtcggc gccttcctct cgggcgccgt gatcgatggc agctggttcg
     4081 accgcgagca gatggatcgc ctgcgccaca acgtgctgct ggtggtgatg ccggtgttct
     4141 tcctcagcac gggcctgcgc accaactgga ccctgggcgg cgcgaccgtg ttcatcgccg
     4201 ccggcgtgct gctgttcgcc gcggtcgcgg gcaagctggc cggcgtgcac ctggccggcc
     4261 gcatccttcg ctgggagcgc ggcgaggcct cgatcatcgg ctggctgctg cagaccaagg
     4321 ccctgatcat gatcatcttc gccaacatcc tgctcgacaa ggcgatcatc accagcga
//
LOCUS       DAORUK010000165         4308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_25981_length_4308_cov_17.370092, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000165 DAORUK010000000
VERSION     DAORUK010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25981_length_4308_cov_17.370092"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /gene="tgt"
                     /locus_tag="PK818_09385"
     CDS             complement(<1..999)
                     /gene="tgt"
                     /locus_tag="PK818_09385"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014508063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQY82980.1"
                     /translation="MSRMSFELLGSDGAARRGRITFPRGTIETPAFMPVGTYGSVKGA
                     LPHQVRELGAEIILGNTFHLFLRPGLAVIEAHGGLHGFARWDKPILTDSGGFQVFSLA
                     HRRKITEAGVTFAAPTDGSKVFLGPEESMHIQKVLGSDIVMIFDECPPVHVDGAPVDK
                     RVIERSMELSLRWAERSKRAHEGNDAALFGIVQGGVHHDLRSRSAEGLQQVGFDGYAI
                     GGLAVGETEAERNAMLEHTAPQLPEDRPRYLMGVGRPEDLVEAVARGVDMFDCVMPTR
                     NARNGHYFTSTGTIRVRNAQYERDLRPIEEGCDCHACANGFSRAYLRHLDRCNEMLA"
     gene            complement(1138..2181)
                     /gene="queA"
                     /locus_tag="PK818_09390"
     CDS             complement(1138..2181)
                     /gene="queA"
                     /locus_tag="PK818_09390"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HQY82981.1"
                     /translation="MKKSDFHYQLPAELIAQAPLPERSASRMLLVPPGDAAFADLGVR
                     DLPALLQPGDLLVFNDTRVIPARLFGNKATGGRVEILVERLLPGNQARAQLGVSKPPQ
                     PGSRIALDAGGEAEVLSRDDAGFWQLRFHLPEPLENWLLHAGRLPLPPYIQREPGADD
                     AERYQTVFAREVGAVAAPTAGLHFDDALLAALRERGVRFGHVTLHVGAGTFQPMRVDD
                     IREHRMHSEWINVGAELVQQVRRTREAGGRVIAVGTTVLRALESALVDGDLQPFAGET
                     NIFIFPGYRIRSIDALLTNFHLPESTLLMLVSAFAGKSRVFAAYQHAVRERYRFFSYG
                     DAMLLYPETRDAQ"
     gene            complement(2226..2657)
                     /locus_tag="PK818_09395"
     CDS             complement(2226..2657)
                     /locus_tag="PK818_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003489775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HQY82982.1"
                     /translation="MRITPADELLLSLLREDARASTADIARRLGLSRTTVQNRIARLE
                     AQGVIRGYTVRVHDDYEQSRIRAHILITVRPKQMPAVVKALQGVREVRVLYSISGGYD
                     LIALAVAGSVGEMDVLTDAIGAIDGVERTTTSIILSTKFER"
     gene            2797..4305
                     /locus_tag="PK818_09400"
     CDS             2797..4305
                     /locus_tag="PK818_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQY82983.1"
                     /translation="MTLLDTLAPLRAHGNTRTTGLDDATILAFAARDPQLAEAIHAAA
                     AEYAQVRADFPGLLDLDEDAQRAEVQAGFVNFYSQDNANPYVALTARGPWIVTLKGAV
                     VYDTGGYGMLGLGHAPQAVIDAMAKPQSMANIMTPHVAQLRFSNAMRREIGQTRGGCP
                     YAAFLCLNSGSESVSLAARIVDANAKTMTDAGARHAGKAIKRLVVKGAFHGRTEKPAL
                     YSDSTRKTYVQHLASFRGEHSVIAVAPYDMDALRQAFAEADSNGWFIEAMFLEPVMGE
                     GDPGRSVPVAYYNLARELTRAHGALLLVDSIQAGLRATGYLSIVDYPGFEHSDAPDME
                     TYSKALNAGQFPLSVLAVTESAAAIYKRGLYGNTMTANPRALDVACAVLAQLTPRLRA
                     NIQQRGTEVLAMLDTLKEELGGRITKVQGTGLLFSCELAAGYKGYGTGSTEEWMREQG
                     IGVIHGGENSLRFTPHFAVTSAELELVVAMVRRALLEGPRQQLDASQAAAAA"
BASE COUNT          690 a         1503 c         1480 g          635 t
ORIGIN      
        1 cgccagcatc tcgttgcaac ggtccaggtg gcgcaggtag gccctgctga agccgttggc
       61 gcaggcgtgg cagtcgcagc cctcctcgat cgggcgcagg tcgcgctcgt actgggcgtt
      121 gcgcacgcgg atggtgccgg tggaggtgaa atagtggccg ttgcgcgcgt tgcgggtcgg
      181 catcacgcag tcgaacatgt cgaccccgcg cgccaccgcc tccaccaggt cttccggccg
      241 gcccaccccc atcaggtagc gcgggcggtc ttcgggcagt tgcggcgcgg tgtgctcgag
      301 catggcgttg cgttcggcct cggtctcgcc gaccgccagc ccgccgatcg cgtagccgtc
      361 gaagccgacc tgctgcaggc cctcggccga gcgcgagcgc aggtcatggt ggacgccgcc
      421 ctggacgatg ccgaacagcg ccgcgtcgtt gccctcgtgc gcgcgcttcg aacgttcggc
      481 ccagcgcagc gacagctcca tcgaacgctc gatgacgcgc ttgtcgaccg gcgcgccatc
      541 gacatgcacc ggagggcatt catcgaagat catcacgatg tcgctgccca gcaccttctg
      601 gatgtgcatg gattcctcgg gaccgaggaa caccttgctg ccgtcggtgg gcgcggcgaa
      661 ggtcacgccg gcctcggtga tcttgcgccg gtgcgccagc gagaacacct ggaagccgcc
      721 ggaatcggtg aggatcggct tgtcccagcg cgcgaagccg tgcaggccgc cgtgggcctc
      781 gatcaccgca agcccgggcc gcaggaacag gtggaaggtg ttgccgagga tgatctccgc
      841 tccgagttcg cgcacctggt gcggcaacgc gcccttgacc gagccgtagg tgccgaccgg
      901 catgaacgcc ggggtctcga tggtgccgcg cgggaaggtg atgcggccgc ggcgggcggc
      961 gccatcggat ccaagcagct cgaaagacat gcggctcatg cgttgtcgcc tgcggcagtg
     1021 ccggccatcg ggtactccgg aaagcgggcc atggatggcc cgcggcggcg catggaacag
     1081 gacgttcccc tgcgccgggc aggagtaccc gatggccggc actgccgcgt gcaaggttca
     1141 ctgcgcatcg cgcgtctccg ggtacagcag catcgcatcg ccatagctga agaagcgata
     1201 gcgctcgcgc accgcatgtt ggtaggcggc gaacacgcgc gacttgccgg cgaacgcgga
     1261 caccagcatc agcagcgtgc tttccggcag gtggaaattg gtcagcagcg catcgatgct
     1321 gcgaatgcgg tagccgggga agatgaagat attggtctcg ccggcgaacg gctgcaggtc
     1381 gccgtcgacc agcgcactct ccagcgcgcg cagcacggtg gtgccgacgg cgatcacccg
     1441 cccgcccgcc tcgcgcgtgc gccgcacctg ctgcaccagc tcggcgccca cgttgatcca
     1501 ctcgctgtgc atgcggtgct cgcggatgtc gtccacccgc atcggctgga aggtgccggc
     1561 gccgacatgc agggtgacgt ggccgaaccg caccccgcgc tcgcgcaacg ccgcgagcag
     1621 cgcgtcatcg aaatgcaggc cggcggtggg cgcggcgact gcgccgactt cgcgcgcgaa
     1681 cacggtctgg taacgctccg catcgtccgc gccgggctcg cgctggatgt agggcggcag
     1741 cggcaggcgg ccggcatgca acagccagtt ctccagcggc tccggcaagt ggaagcgaag
     1801 ttgccagaag ccggcgtcat cgcgcgacaa cacttccgcc tcgccgccgg catccagggc
     1861 gatccggctg ccgggctgcg gcggcttgct cacgcccagc tgcgcgcgcg cctggttgcc
     1921 gggcagcagg cgttcgacca ggatctcgac ccgcccgccg gtggccttgt tgccgaacag
     1981 ccgcgccggg atcacccggg tgtcgttgaa taccagcagg tcgccgggct gcagcagtgc
     2041 cggcaggtcg cgcaccccga ggtcggcgaa cgcggcatca cccggcggga ccagcagcat
     2101 gcggctggcg gaacgctccg gcagcggtgc ctgcgcgatc agctccgcgg gcagttggta
     2161 atggaagtcc gacttcttca aggtgctgca accatccaag gccgcgcatt gtaggcgagc
     2221 ggcgctcagc gctcgaactt ggtggacagg atgatcgagg tggtggtgcg ctcgaccccg
     2281 tcgatcgcac cgatggcatc ggtcagcacg tccatctcgc cgaccgagcc ggccaccgcc
     2341 agcgcgatca ggtcgtagcc gccgctgatc gagtacagca cccgcacctc gcgcacgccc
     2401 tgcagggcct tgaccaccgc cggcatctgc ttgggccgca cggtaatcag gatgtgcgcg
     2461 cggatccggc tctgttcgta gtcgtcgtgc acgcgcacgg tatagccgcg gatcacgccc
     2521 tgcgcctcca gccgcgcgat ccggttctgc accgtggtcc gcgacagccc gagccggcgc
     2581 gcgatgtccg cggtggaggc gcgcgcatcc tcgcgcagca gggacagcag cagttcgtcg
     2641 gcaggggtaa ttcgcatatc gccggatgat accgttgatt cgacggaata gccccgttat
     2701 tcggccggat catccctttc gactggtcgg ccgacggcga taaagtggcg gttctgcatt
     2761 cgcgcacgcg caccgcgcgc caaggaccaa gccgccatga ccctgctcga caccctcgcc
     2821 ccgctgcgcg cgcacggcaa cacccgcacc accggcctgg acgatgccac catcctcgcg
     2881 ttcgccgcgc gcgacccgca gctggccgaa gcgatccacg ccgccgccgc cgaatacgcg
     2941 caggtgcgcg ccgacttccc cggactgctg gacctggacg aggacgcgca gcgcgcggaa
     3001 gtgcaggccg gcttcgtcaa cttctattcg caggacaacg ccaatccgta cgtggcgctg
     3061 accgcgcgcg ggccgtggat cgtgaccctg aagggcgcgg tggtgtacga caccggcggc
     3121 tacggcatgc ttggcctggg ccatgcgccg caggcggtga tcgatgcgat ggccaagccg
     3181 cagtcgatgg ccaacatcat gaccccgcac gtggcgcagc tgcgcttctc gaatgcgatg
     3241 cggcgggaaa tcggacagac ccgcggcggc tgcccgtacg ccgcgttcct gtgcctgaac
     3301 tcgggttcgg aatcggtctc gctggcggca cgcatcgtcg acgccaatgc caagaccatg
     3361 accgacgcgg gcgcgcgcca cgccggcaag gcgatcaagc ggctggtggt caagggcgcg
     3421 ttccacggcc gcaccgagaa gcctgcgctg tactccgact ccacccgcaa gacctacgtg
     3481 cagcacctgg ccagcttccg cggcgagcac tcggtgatcg cggtggcgcc gtacgacatg
     3541 gacgcgctgc gccaggcgtt cgccgaagcg gacagcaacg gctggttcat cgaggcgatg
     3601 ttcctggaac cggtgatggg cgaaggcgac cccggccgca gcgtgccggt ggcgtactac
     3661 aacctggcac gcgagttgac ccgcgcgcat ggcgccctgc tgctggtcga ttcgatccag
     3721 gccggcctgc gcgccaccgg ctacctgtcg atcgtcgatt accccggctt cgaacactcc
     3781 gatgcaccgg acatggagac ctattcgaag gcgctcaacg ccggccagtt cccgctgtcg
     3841 gtgctcgcgg tcaccgagtc ggcggcggcc atctacaagc gcggcctgta cggcaacacg
     3901 atgaccgcca acccgcgcgc gctggacgtg gcctgcgcgg tgctggcgca gctcaccccg
     3961 cggctgcgcg ccaacatcca gcaacgcggc accgaggtgc tggcgatgct ggacacgctg
     4021 aaggaagaac tcggcggccg catcaccaag gtccagggca ccggcctgct gttctcctgc
     4081 gaactggcgg ccggctacaa gggctacggc accggttcca ccgaggagtg gatgcgcgaa
     4141 cagggcatcg gcgtgatcca cggcggcgag aactcgctgc gcttcacccc gcacttcgcg
     4201 gtcaccagcg cggaactcga actggtggtc gcgatggtca ggcgcgcgct gctggaaggc
     4261 ccgcgccagc aactcgacgc cagccaggca gccgcggctg cctgagcc
//
LOCUS       DAORUK010000166         4294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_26130_length_4294_cov_19.148620, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000166 DAORUK010000000
VERSION     DAORUK010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26130_length_4294_cov_19.148620"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PK818_09405"
     CDS             <1..418
                     /locus_tag="PK818_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007972236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY82984.1"
                     /translation="KMESFDGIQENIALVDRAPGGCAIVHSDSAEGIQRLNQEAAKVI
                     ASAGRAGIAITPEHAIRWITQNPAKAMGILEQTGTLEAGKMADVVLWNGNPFSSYAQA
                     EQVYVDGARMYDRRDPARQPKSDFLLGQDLLRGGVR"
     gene            415..1707
                     /locus_tag="PK818_09410"
     CDS             415..1707
                     /locus_tag="PK818_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005913553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQY82985.1"
                     /translation="MSAASLKRLAVAVALLATTGIASAQDLLIRNATVHTASARGTLQ
                     AADVLVRNGRIAAVGSGLAAPGTSVLDAQGKPLTPALFGGVTDIGVEEVSGESATVDN
                     TLALGANAADMQVRPEFDVTLAYNPESVLVPVARVEGIGWTLLSAGTRAGGSFIGGQG
                     GAVRLDGSLDAIGPRTLFMTLGGEAASLSGSSRAAQWMLLDQLVDEVRGRIPADSRFA
                     LLTPAGRTTLARYFGGGGRVLVRIQRAADITRLLRWSKQRGVSIALLGAAEGWKVAPQ
                     LAAAGVPVFVDALGNLPNDFDEIGATLENAARLHAAGVRVSFSQAGDASHNARKIRQL
                     AGNAVANGLPWEAALAGLTSVPASSFGVGNEVGSIAVGQRADLVLWSGDPLDVASVAR
                     QVWLDGRAIPMRSRQTELRDRYLRPGGDLPRAYPDAAR"
     gene            1775..2179
                     /locus_tag="PK818_09415"
     CDS             1775..2179
                     /locus_tag="PK818_09415"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HQY82986.1"
                     /translation="MPNFIDINWLAVLVAAVSAFLLGGIWYGPLFKHAWCRENGIDPD
                     APPKGHPGMVFGTAFVAALVAAAAFAIILRPDPPLFIALHAGFLTGLAYVAMSFGINY
                     AFAGRSFRLWLIDGGYHTLQFSLYGLILGLWH"
     gene            2294..3238
                     /locus_tag="PK818_09420"
     CDS             2294..3238
                     /locus_tag="PK818_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HQY82987.1"
                     /translation="MRIGIVVDSACDVPSDFIAQEEITILPVTVQIGNAVLADMRNEE
                     ATLNFLTGDTAAQAYNAETTPYTVQQVHDLFLGRLVLDYDYVFCICTTRSRSGIFDNA
                     QQASFTILNDYKPVRAAIGNTTPFSLRVIDSQNVFAAVGVMAVEAARLRAAGEGAPKI
                     RSRLEHLALYTQGYMVVPDLHYLRNRIKKRGDKSVSFFSATLGTALDIKPILHCNRGD
                     TAPAAKVKGFTNAAEKLFDFAGSRVRAGLMTPTMCLSFGGELDRMRDLPGYQRLRDTC
                     AEHNVEVFESVMSLSGMVNVGQGALVVGFAAEPAKLEV"
     gene            3297..3641
                     /locus_tag="PK818_09425"
     CDS             3297..3641
                     /locus_tag="PK818_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010480863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82988.1"
                     /translation="MPTRYYISLPDPANARGSDAALAFRARGAEAFAAELQDALRNDA
                     LFQHWRARQDDPDGVDPVLGATDAHAVVSGQQQDLHVDLVVTTSLPSAVLRQRLGLLA
                     GHHWQLRDVTAG"
     gene            3778..>4294
                     /locus_tag="PK818_09430"
     CDS             3778..>4294
                     /locus_tag="PK818_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82989.1"
                     /translation="MPVVALGRALMLVLLSACAPTTQAPVAPAATARASTAAATMAPA
                     HAVDHAPSLDGVRGNWRVVAGDDPGDQAIMAISLLSADEAASANGDYVLFQPVCDVVD
                     GQPVEGTSDCELIGQTGTFERIELLGDRAVLAFSPTADGAEHRLELQLDGDALAGHYV
                     AEGNDVRRAVRA"
BASE COUNT          639 a         1517 c         1503 g          635 t
ORIGIN      
        1 caagatggaa agcttcgacg gcatccagga gaacatcgcg ctggtcgacc gcgcgcccgg
       61 tggctgcgcg atcgtgcatt cggattcggc cgaaggcatc cagcggctca accaggaagc
      121 ggccaaggtg atcgccagcg cggggcgcgc cgggatcgcc atcaccccgg aacacgcgat
      181 ccgctggatc acccagaacc cggccaaggc gatgggcatc ctcgagcaga ccggcaccct
      241 ggaggccggc aagatggccg acgttgtcct gtggaacggc aacccgttca gcagctacgc
      301 gcaggccgaa caggtgtacg tggatggcgc gcggatgtat gaccgccgcg atccggcgcg
      361 gcaaccgaag tcggacttcc tgcttggcca ggacctgctg cgtggaggcg tgcgatgagc
      421 gccgcgtccc tgaagcgcct cgcggtggcg gtcgcgctgc tggccacgac cggcatcgcc
      481 tccgcgcagg acctgctgat ccgcaacgcc accgtgcata ccgccagtgc gcgcggcacc
      541 ctgcaggcgg ccgacgtgct ggtgcgcaat ggccgcatcg ccgcggtcgg cagcggcctg
      601 gccgcgcccg gcaccagcgt gctggacgcg cagggcaagc cgctgacccc ggcgctgttc
      661 ggcggcgtca ccgacatcgg ggtggaagag gtttccggcg agtccgccac cgtcgacaac
      721 accctcgcgc ttggcgccaa cgccgccgac atgcaggtgc gcccggagtt cgacgtgacc
      781 ctggcctaca acccggaatc ggtgctggtg ccggtggcgc gggtggaagg catcggctgg
      841 acgctgctgt cggcgggcac ccgtgccggc ggctccttca tcggcggcca gggcggcgcg
      901 gtgcggctgg acggcagcct ggacgcgatc ggcccgcgca ccctgttcat gaccctgggt
      961 ggcgaagccg cttcgctcag cggcagctcg cgcgcggcgc agtggatgct gctggaccag
     1021 ctcgtcgacg aggtgcgcgg acggatcccg gcggactccc gcttcgccct gctcaccccg
     1081 gccgggcgca ccaccctggc ccgctatttc ggcggcggtg gccgcgtgct ggtgcgcatc
     1141 cagcgtgccg ccgacatcac ccgcctgctg cgctggtcga agcagcgcgg ggtcagcatc
     1201 gccctgctcg gcgccgccga agggtggaag gtcgcgccgc aactggccgc cgccggggtg
     1261 ccggtgttcg tggatgcgct cggcaacctg ccgaacgact tcgacgagat cggcgccacc
     1321 ctggaaaacg cggcgcgcct gcacgcggct ggcgtgcggg tctcgttctc gcaggccggc
     1381 gatgcctccc acaacgcgcg caagatccgc cagctggcgg gcaacgcggt ggccaacggg
     1441 ctgccgtggg aggccgcgct ggccgggctg acctcggtgc cggccagcag cttcggcgtc
     1501 ggcaatgaag tgggcagcat cgccgtgggc cagcgcgccg acctggtgct gtggagcggc
     1561 gatccgctcg acgtcgccag cgtcgcccgc caggtgtggc tggacggccg cgcgatcccg
     1621 atgcgatcac gccagaccga actgcgcgac cgctacctgc ggcccggcgg cgacctgccg
     1681 cgtgcgtatc cggacgccgc gcgctgacct tcgcgcaacc tggtaaaggc cgcgcggcgt
     1741 aagctcgcgg ccgggcaccc tggggagaca tggcatgccg aacttcatcg acatcaactg
     1801 gctggcggtc ctggtggcgg cggtgtccgc cttcctgctt ggcgggatct ggtacggacc
     1861 gttgttcaag cacgcatggt gccgggagaa cggcatcgac cccgacgcgc cgccgaaggg
     1921 ccacccgggc atggtgttcg gcaccgcgtt cgtcgccgcg ctggtcgcgg ccgctgcgtt
     1981 cgcgatcatc ctgcgcccgg acccgccgct gttcatcgcc ctgcacgccg gcttcctgac
     2041 cggcctggcc tacgtggcga tgagcttcgg catcaactac gcgttcgccg gccgcagctt
     2101 ccggctgtgg ctgatcgatg gcggctatca caccctgcag ttcagcctgt acgggctgat
     2161 cctgggcctc tggcactgag ccgggcattg acgtggtggg acgccgcgcg cccgaccatg
     2221 ccgctttgcc acatgcatcg gcgcgcgact tgcggtacgg tcggcgcatc ttgaccgggg
     2281 ggtggaggac gacatgcgca taggcatcgt ggtggattcg gcgtgcgacg taccaagcga
     2341 tttcatcgcg caggaggaga tcaccatcct gccggtgacc gtgcagatcg gcaacgcggt
     2401 gctggcggac atgcgcaacg aggaggcgac gctcaacttc ctcaccggcg acaccgcggc
     2461 ccaggcctac aacgccgaga ccacgccgta caccgtgcag caggtgcacg acctgttcct
     2521 gggccggctg gtgctggact acgactacgt gttctgcatc tgcaccacgc gctcgcgcag
     2581 cggcatcttc gacaacgcgc agcaggccag cttcaccatc ctcaacgact acaagccggt
     2641 gcgtgccgcg atcggcaaca ccaccccgtt ctcgctgcgg gtgatcgaca gccagaacgt
     2701 gttcgcggcg gtcggggtga tggcggtgga agccgcgcgc ctgcgcgccg ccggcgaggg
     2761 cgcgccgaag atccgctcgc gcctcgagca cctggccctg tacacccagg gctacatggt
     2821 ggtgccggac ctgcactacc tgcgcaaccg gatcaagaag cgtggcgaca agagcgtcag
     2881 cttcttcagc gcgacgctcg gcaccgcgct cgacatcaag ccgatcctgc attgcaaccg
     2941 tggcgatacc gcgcccgcgg ccaaggtgaa gggcttcacc aacgccgccg agaaactgtt
     3001 cgacttcgcc ggcagccggg tacgcgcggg gctgatgacc ccgaccatgt gcctgagctt
     3061 cggtggcgaa ctggaccgca tgcgcgacct gcccggctac cagcgcctgc gcgacacctg
     3121 cgccgagcac aacgtcgagg tgttcgagag cgtgatgagc ctgtcgggca tggtcaacgt
     3181 cggccagggc gcgctggtgg tgggtttcgc cgcggagccg gccaagctcg aggtctgaac
     3241 gcggcgctaa cgtgcgcccg ctacgctgtg cgcttcgcca tgccgacgga gcgcacatgc
     3301 cgacgcgtta ctacatcagc ctgcccgatc ccgccaacgc gcgcggcagc gacgccgcgc
     3361 tcgcgttccg cgcccgcggc gcggaggcgt tcgccgccga actgcaggac gcgctgcgca
     3421 acgacgcact gttccagcac tggcgcgcgc gccaggacga cccggacggg gtcgacccgg
     3481 tgctcggcgc aaccgacgcc catgcggtcg tcagcggcca gcagcaggac ctgcacgtcg
     3541 acctggtggt gaccacttcg ttgccctcgg cggtgctgcg ccagcgcctc gggctgcttg
     3601 ccggccacca ctggcaactg cgcgacgtga ccgcgggttg agcacggaac gcggggtggc
     3661 acgcagaccc tcgctgctgt cgtggagcgg cggcaaggac gcgggcgggc ccggcatgtc
     3721 cgctttggca tccagccttc gtaacccatt actggtcccg ccaataaacc atcgtccatg
     3781 cccgttgttg ccctgggtcg agcgctgatg ctcgtcctgc tttccgcctg cgcacccacg
     3841 acacaggctc ccgtcgcacc agcggcaacc gcccgggcat ccaccgccgc cgcgaccatg
     3901 gcgcctgcgc acgcggtcga tcacgcgccc tccctcgacg gcgtgcgcgg caactggcgc
     3961 gtggttgccg gggacgatcc cggtgaccag gcgatcatgg cgatttcact cctgtccgcc
     4021 gacgaggcgg cgtccgccaa tggcgactac gtcctgttcc agccggtctg cgacgtggtg
     4081 gacgggcagc cggtggaggg gacctcggac tgcgaactga tcggccagac aggaacgttc
     4141 gagcgcattg agctgctcgg ggaccgcgcg gtgctggcct tcagccccac cgcggatggc
     4201 gcggagcacc gcctggagtt gcagctggat ggcgatgcct tggcgggcca ttacgtggcg
     4261 gaaggaaacg atgtccgccg tgcggtgcgc gccg
//
LOCUS       DAORUK010000167         4293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_26139_length_4293_cov_19.091789, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000167 DAORUK010000000
VERSION     DAORUK010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26139_length_4293_cov_19.091789"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PK818_09435"
     CDS             <1..673
                     /locus_tag="PK818_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014501853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQY82990.1"
                     /translation="SMDPGFSVTTVSLLDRGMVYALAHIRGGQEMGRAWYEDGKLLNK
                     KNTFTDFIDVTGFLVKQGYAAPDRVAAHGGSAGGLLMGAVANMAPDAYRVILSQVPFV
                     DVVTTMLDPTIPLTTNEYDEWGNPEQKPFYDYMLTYSPYDNLAKKAYPATFVGTGLWD
                     SQVQYWEPAKYVARLRDLNTGSEPVVFRINMEAGHGGKSGRFRRYREQAEMYSFMLDR
                     LGVGK"
     gene            698..1057
                     /locus_tag="PK818_09440"
     CDS             698..1057
                     /locus_tag="PK818_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007965023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HQY82991.1"
                     /translation="MRWLWWLLAYASLGIGIVGIVVPGLPTTPFVLLAAFAAARGSRR
                     MHAWLLAHRTFGKIIRDWQREGAVSRQAKRLSSGTMVVCALVLVLTAPKWWMAAIGCT
                     FMACVSAWLWRRPEPRQ"
     gene            1105..1266
                     /locus_tag="PK818_09445"
     CDS             1105..1266
                     /locus_tag="PK818_09445"
                     /inference="COORDINATES: protein motif:HMM:NF025012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HQY82992.1"
                     /translation="MNRLAATLLLVLALAACGNKGPLVKPEAKPAATPATTTPAAEPA
                     KSEAGTPVR"
     gene            1275..2120
                     /gene="dapF"
                     /locus_tag="PK818_09450"
     CDS             1275..2120
                     /gene="dapF"
                     /locus_tag="PK818_09450"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HQY82993.1"
                     /translation="MRGLKLAFSKMHGAGNDFVVLDLRDGRPAPDPALCARIADRHLG
                     VGCDQLLTVEAPRSADAVAGYRIWNADGSAAGQCGNGARCIAAWLVRDGTAPATAGFA
                     IDSPSATHAVARDADAGFVIDMGTPRFAPQAVPLLGWDAAEDAYALAIDGGTVRFGAV
                     SMGNPHAVIEVAEVAHAPVAAIGAALQRAPAFPQSVNAGFAEVVARDRIRLRVFERGV
                     GETLACGSGACAAVAVLVRGGRIDADREVAVELPGGTLRIRYDRAAERIHMGGPTAFV
                     FEGEW"
     gene            2126..2803
                     /locus_tag="PK818_09455"
     CDS             2126..2803
                     /locus_tag="PK818_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HQY82994.1"
                     /translation="MDFDKHAEKLGAHEVAAWLRRHPDFLQKFPDLALTMVVPRENGP
                     AASLASYQLEVLRDKNRELSRRLQELFGNAQENERLAVRTHQLTLALMKQASAADTLR
                     AMAASLAEDFQGDLVRIVAFEAVDGIDDADWLQVIAEDDPRLSGFRDLAAGGEPVCGR
                     LHPDKNAVLYGLRAEEVQGSALLVLPGTGLVAVGSRDPNRFFPGMGTLFLRMMGEALV
                     AALQRFR"
     gene            2889..3773
                     /gene="xerC"
                     /locus_tag="PK818_09460"
     CDS             2889..3773
                     /gene="xerC"
                     /locus_tag="PK818_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HQY82995.1"
                     /translation="MVAEFLAHLAVERRMSAHTLDAYRRDLAALAAWAQAQARALPGL
                     HGDDLRAFVAAEHRRGLSPKSLQRRLSACRSFYRWLLKLGRIAASPADAIRAPKAARR
                     LPQVLDVDEAVQLVEVSTDAPLGLRDRALLELLYSSGLRLSETCALKWGDLDFGQGLV
                     TVLGKGGKQRVVPVGSHARNALQAWRAESGGQQAGFVFPGRGGGQISARAVQLRVKRL
                     AMQRGVFKRVHPHLLRHSFASHMLESSGDLRGVQELLGHADIATTQIYTHLDFQHLAR
                     VYDAAHPRAKRKPGNGDG"
     gene            3818..4078
                     /locus_tag="PK818_09465"
     CDS             3818..4078
                     /locus_tag="PK818_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82996.1"
                     /translation="MVETIEGWRCIGCGRVDAPRPCIGVCQDKRVELVLAEDYAELAW
                     RVQQLEEALALIARTTPKPDKLDASWQALQARARALLGSGSG"
     gene            4148..>4293
                     /locus_tag="PK818_09470"
     CDS             4148..>4293
                     /locus_tag="PK818_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011260360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82997.1"
                     /translation="MDPSQNPNVFHATTIVCVRRNGQVVIAGDGQVTLGHTVMKANAR
                     KVRRL"
BASE COUNT          604 a         1441 c         1622 g          626 t
ORIGIN      
        1 gtcgatggat ccgggcttct cggtgaccac ggtcagcctg ctggatcgcg gcatggtcta
       61 cgcgctggcg catatccgcg gtggccagga gatgggccgc gcgtggtacg aggacggcaa
      121 gctgctcaac aagaagaaca ccttcaccga cttcatcgac gtcaccggtt tcctggtgaa
      181 gcagggctat gccgcgcccg accgcgtcgc cgcgcacggc ggcagcgccg gtggcctgct
      241 gatgggtgcg gtcgccaaca tggcgccgga cgcgtaccgg gtgatcctgt cgcaggtgcc
      301 gttcgtggac gtggtgacga ccatgctgga tccgaccatc ccgctgacca ccaacgaata
      361 cgacgaatgg ggcaatcccg agcagaagcc gttctacgac tacatgctga cctactcgcc
      421 gtacgacaac ctggcgaaga aggcgtatcc cgcgaccttc gtgggcaccg gcctgtggga
      481 ttcgcaggtg cagtactggg agccggcgaa gtacgtggcg cgcctgcgcg acctcaacac
      541 cggatcggag ccggtggtgt tccggatcaa catggaagcc ggccatggcg gcaagtccgg
      601 ccgcttccgc cgctaccgcg aacaggcgga gatgtattcg ttcatgctcg accggctggg
      661 cgtcggcaag taggcggccg cggccggcgg cgcgcggatg cgctggctct ggtggctgct
      721 ggcctacgcc agcctgggca tcggcatcgt cggcatcgtg gtgccggggc tgccgaccac
      781 gccgttcgtg ctgctggcgg cgttcgcggc ggcgcgcggc tcgcggcgga tgcacgcgtg
      841 gctgctggcg caccgcacgt tcgggaagat catccgcgac tggcagcgcg agggcgcggt
      901 ctcgcggcag gcgaagcggc tgtccagcgg caccatggtg gtgtgcgcgc tggtgctggt
      961 cctgaccgcg ccgaagtggt ggatggccgc catcggctgc accttcatgg cctgcgtctc
     1021 ggcctggctg tggcgccggc cggagccgcg acagtgacgc gaaggcgacg cgacagtgca
     1081 tcggggcgcg gttacactgc ccgcatgaac cgtcttgccg ccacgctgtt gctggtcctc
     1141 gcccttgccg cctgcggcaa caagggcccg ctggtgaagc cggaagccaa gccggccgca
     1201 acgcccgcca ccaccacgcc cgccgccgaa cctgcgaagt ccgaggccgg cacgccggtg
     1261 cgctgaaggc cgcgatgcgc ggcttgaagc tggctttctc gaagatgcat ggcgcgggca
     1321 acgacttcgt cgtgctcgac ctgcgcgatg gccggcccgc gccggatccc gcgctgtgcg
     1381 cgcgcatcgc cgaccgccac ctgggggtcg gctgcgacca gctgctgacg gtggaggcac
     1441 cgcgcagcgc ggatgcggtg gccggctacc ggatctggaa tgcggatggc tcggccgccg
     1501 ggcaatgcgg caacggcgcg cgttgcatcg ccgcgtggct ggtgcgcgac ggcaccgccc
     1561 cggccaccgc cggtttcgcg atcgacagtc cgtccgccac ccacgcggtg gcgcgggatg
     1621 ccgacgccgg cttcgtgatc gacatgggca ccccgcggtt cgcgccgcag gcggtgccgc
     1681 tgctgggctg ggacgccgcc gaagacgcct acgcgctcgc catcgatggc ggcacggtcc
     1741 gcttcggcgc ggtgtcgatg ggcaacccgc atgcggtgat cgaagtcgcg gaggtcgcgc
     1801 acgcaccggt ggcggcgatt ggcgcggcgc tgcagcgcgc gccggcattc ccgcaatcgg
     1861 tgaatgcggg gttcgccgag gtggtcgcgc gcgaccgcat ccgcctgcgc gtgttcgagc
     1921 gcggcgtcgg cgaaaccctg gcctgcggca gcggcgcctg cgcggcggtg gcggtgctgg
     1981 tgcgcggcgg gcgcatcgac gccgaccgcg aggtggcggt ggagctgccc ggcggcaccc
     2041 tgcgcatccg ctacgatcgg gccgcggagc gcatccacat gggcgggccg accgcattcg
     2101 tgttcgaagg ggagtggtga tggcgatgga tttcgacaag cacgcggaaa agctgggcgc
     2161 gcatgaagtg gcggcctggc tgcgccggca cccggacttc ctgcagaagt tccccgacct
     2221 tgcgctgacc atggtggtcc cgcgcgagaa cggcccggcg gcgtcgctgg ccagctacca
     2281 gctggaggtg ctgcgcgaca agaaccgcga gctgtcgcgg cggctgcagg agctgttcgg
     2341 caacgcgcag gagaacgaac gcctggcggt gcgtacccac cagctcaccc tggcgctgat
     2401 gaagcaggcc agcgcggcgg atacgctgcg cgcgatggcc gcctcgctgg cggaggattt
     2461 ccagggcgac ctggtgcgca tcgtggcgtt cgaggcggtc gatggcatcg acgacgcaga
     2521 ctggctgcag gtgatcgccg aagacgaccc gcggctgtcc ggcttccgcg acctcgccgc
     2581 cggcggcgaa ccggtgtgcg gccgcctgca tccggacaag aacgcggtgc tgtatggcct
     2641 gcgcgcggag gaggtgcagg gttcggcgct gctggtgttg cccggcaccg gcctggtggc
     2701 ggtcggcagc cgcgatccca atcgcttctt cccgggcatg ggcacgctgt tcctgcgcat
     2761 gatgggcgag gcgctggtgg cggcgctgca gcgcttccgt tgatggcgtg aacgccgcac
     2821 gcgcgcagca gcaagcggcc acccgcgctg gcgatggcgg gcaggcgcag gccgcggggg
     2881 tccccgcgtt ggtcgcggag ttcctggcgc acctggcggt cgagcggcgg atgtcggcgc
     2941 acacgctgga cgcctaccgc cgcgaccttg cggcgctggc cgcatgggcg caagcgcaag
     3001 cgcgggcgct gcccggactg cacggcgacg acctgcgcgc gttcgtcgcc gccgagcatc
     3061 gccgcggatt gtcgccgaag agcctgcagc gccggctgtc cgcctgccgc agcttctacc
     3121 gctggctgtt gaagcttggc cggatcgccg ccagtccggc cgatgcgatc cgcgcgccga
     3181 aggccgcgcg caggttgccg caggtgctcg acgtcgacga ggcggtgcaa ctggtggagg
     3241 tgtccaccga cgcgccgctc ggcctgcgcg accgcgccct gctggagctg ctctattcct
     3301 ccggcctgcg cctgtcggaa acctgcgcac tgaagtgggg cgacctcgat ttcggccagg
     3361 gcctggtcac ggtgctgggc aagggcggca agcagcgggt ggtgccggtg ggctcgcacg
     3421 cgcgcaacgc gctgcaggcg tggcgcgccg aatccggcgg ccagcaggcc ggcttcgtgt
     3481 tccccgggcg cggcggcggg cagatcagcg cgcgcgcggt gcagctgcgg gtcaagcggc
     3541 tggcgatgca gcgcggggtg ttcaagcgcg tgcacccgca cctgctgcgg cacagcttcg
     3601 ccagccacat gctggaatcc tcgggcgacc tgcgcggggt gcaggaattg ctggggcatg
     3661 ccgacatcgc caccacccag atctacaccc acctggattt ccagcacctg gccagggtct
     3721 acgacgccgc gcatccgcgg gcgaagcgca agccgggcaa cggcgacggt tgatccggat
     3781 cattgccgtg cgcgcggcgg ggggcgcacc atggcgcatg gtcgagacga tcgaaggctg
     3841 gcgttgcatc ggttgcggac gggtggacgc accgcggccc tgcatcggcg tgtgccagga
     3901 caagcgggtc gagctggtgt tggccgagga ttacgccgag ctggcgtggc gggtgcagca
     3961 gctggaagag gcgctggcgc tgatcgcccg caccaccccg aagccggaca agctggatgc
     4021 gtcgtggcag gcgctgcagg cgcgcgcacg ggcgctgctg ggcagcggaa gcggctgagc
     4081 ggctgcctgc gcgatgcttg aagccgggcc gcgcgtcccc acatcgctgg ttcccgcgga
     4141 gagaccgatg gatcccagcc agaaccccaa cgtattccat gccaccacga tcgtctgcgt
     4201 gcgccgcaat ggccaggtgg tgatcgccgg cgacggccag gtgacgcttg gccacacggt
     4261 gatgaaggcc aacgcgcgca aggtgcgccg gct
//
LOCUS       DAORUK010000168         4275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_26311_length_4275_cov_20.196919, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000168 DAORUK010000000
VERSION     DAORUK010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26311_length_4275_cov_20.196919"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="PK818_09475"
     CDS             <1..1109
                     /locus_tag="PK818_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HQY82998.1"
                     /translation="YIHESPKWPQFDWDRNTLSTQLADVRHRQGRLLGRMEALGFALR
                     TEASLVTLTEDVVKSSEIEGERLDRDQVRSSIARRLGIDIGALTPAERDVEGVVEMML
                     DATQRYAEPLTAERLFGWHAALFPTGRSGMQRIIVGAWRDDRTGPMQVVSGPVGRERV
                     HYEAPSADRLAREMGTFVDWFNHEVSLDPVLKAGVAHLWFVTIHPFDDGNGRIARAIA
                     DMALARSERSVQRFYSMSAQIRAEREGYYDILEATQKGELDVTRWLEWFLRCLGRAID
                     GAETTLATVMAKARFWEARAGQSFNERQRLVLNRLLDGFEGKLSSSKWAALAKCSQDT
                     ALRDIDDLVRRGVLARDAGGGRSTSYSLAEIPRG"
     gene            1467..2114
                     /locus_tag="PK818_09480"
     CDS             1467..2114
                     /locus_tag="PK818_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY82999.1"
                     /translation="MSMIAVDSSTLVDTLRFRLAAAKTLPRSAIRIGASVQALVRVSD
                     ADQDALVGRVGEVLRRFIDAAWTVSGVEREADSSGLERVKLAASAQVPARENHNLAER
                     ARRASEEGLAIVDPVVDYRLPGEVIAGATMDLQEKLLLMAMERAERFTAVTGRAWRIG
                     DVQFGVGSEFDDGTSPKLLRREAALLAGGGEDLLTTSERLLLVAAVTLKVGGAVG"
     gene            complement(2310..3470)
                     /locus_tag="PK818_09485"
     CDS             complement(2310..3470)
                     /locus_tag="PK818_09485"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HQY83000.1"
                     /translation="MKVQTSRAGPFTFQLGVDPIALQTPTIRVDDAWQTFRASPLAEV
                     ANQLQREVLVQSVFGTNTIEGATLTLDQTQSALDLDPDKVQGEQQLRVRNIKAAYDHA
                     VAAAENPGWQLSVDYVRAIHARICADLPHPDNRPGLFRDNPRERPTVVGDADHGGQYK
                     PPQFRGDIDRLMDALMDWHRQLVDAGISPLVRAPLVHLYYELIHPFWDGNGRVGRVLE
                     ATILRHAGYKYAPFAMAKYYQEQLHPYFALFNACRKAAATDQPHPNQPFVLFHLEGLR
                     VVIARLQGRVNELVDELLLKNQALGAWNEKLINPRQYAILRKLVDHPGPLTLEALRVD
                     PIYQGLYFRKTEKTRQRDMRHLRDTGLIVVDPSGVLRLGRRHAETASARKPK"
     gene            3722..4183
                     /locus_tag="PK818_09490"
     CDS             3722..4183
                     /locus_tag="PK818_09490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobile mystery protein A"
                     /protein_id="HQY83001.1"
                     /translation="MTQTPAMVRTSLDQRLAPVRIQADALAAPRTGWIRAIREALGMS
                     ATQLARRMGVAQPTLTKLEQSEAADRIQLDSLRRVAAALDCDVVYALVPRRPLQEMVE
                     ARRVLVAGREYARAAHSIALEDRADDEPGVKALHDAAMREVVGDRELWSER"
BASE COUNT          628 a         1192 c         1709 g          746 t
ORIGIN      
        1 cgtacatcca cgagtccccg aagtggcccc agttcgactg ggacaggaac accctgtcca
       61 cccagttggc cgacgtgcgc catcggcaag gccgactcct gggccgaatg gaagcgctcg
      121 ggttcgcgct gcgcaccgag gcctcgctgg tgacgctgac cgaggacgtg gtcaagtcga
      181 gcgagatcga gggtgagcgg ctcgaccggg atcaagtgcg ttcatcgatc gcccgtcgcc
      241 tgggcatcga catcggcgcg ctcacgccgg ccgaacgcga cgtcgagggc gtggtggaga
      301 tgatgctcga tgcgacgcaa cggtacgccg agccgctgac ggccgagcgc ctgttcggct
      361 ggcacgccgc gctctttccc accgggcgca gcggcatgca acggatcatc gtgggcgctt
      421 ggcgcgatga ccgcacgggg ccgatgcagg tcgtgtccgg gcccgtcggg cgcgagcgtg
      481 ttcactacga ggcgccatcg gccgatcggc tggcccgtga gatgggcacg ttcgtggatt
      541 ggttcaacca cgaggtgtcg cttgatccgg tgctgaaggc gggcgtggcg cacctgtggt
      601 tcgtcacgat ccatccgttc gacgatggca atgggcgcat cgcgcgtgcg atcgcggaca
      661 tggcgctggc gcggtccgag cgcagcgtgc agcgcttcta cagcatgtcg gcgcagatcc
      721 gcgcggagcg cgagggttac tacgacatcc tggaagcgac gcagaagggc gagctcgatg
      781 tcacgcgctg gctggagtgg ttcctgcgct gtcttgggcg cgcgatagat ggtgcggaaa
      841 cgacactggc caccgtgatg gcgaaggccc gcttctggga agcccgcgcc gggcaatcgt
      901 tcaacgagcg gcagcggctc gtgctcaatc ggctgctcga tggcttcgag ggcaagttga
      961 gctcatcgaa gtgggctgcg cttgcgaagt gctcgcagga tacggcgctg cgggatatcg
     1021 atgacctggt gcggcgtggc gtgctggcga gggatgccgg gggcgggcgt agtacgagct
     1081 actcccttgc ggagattccg cggggatagc gcgtggcgcg attcgacggc agtgggtgcg
     1141 tcgggtctgt cggctatcgc tgtggggcct gggcgagatt gcggtgttgc ggtcgcgcat
     1201 gagatgcgct cctacagggg cgttccacgt gggcgaaggg tgtggcgggt gggggtggga
     1261 cggtcgccca cggtgtgggc tcctacccgc acccgaggca cttcgttccc cctgtaggag
     1321 cccactccgt gggcgaccgc ggcctcacgc accgctggat cgcctgtgcc ggggtcgcag
     1381 cgccacctcg cgaatcgcag gttctgacac accaccccgg agcataggcc tcgccggcaa
     1441 cccgccggcc tcccggagca attcccatga gcatgatcgc agtcgattcc tcgacgctcg
     1501 tcgacaccct gcgcttccgc ctggcggcgg cgaagacctt gccgcgcagt gcgatccgga
     1561 tcggggcttc ggtgcaggcg ctggtgcggg tgagcgatgc ggaccaggac gcgctggtgg
     1621 ggcgggtggg cgaggtgttg cggcgcttca tcgatgcggc gtggacggtg agcggggtgg
     1681 agcgcgaggc ggatagctcg gggctggagc gggtgaagct ggcggcgtcg gcgcaggtgc
     1741 cggcgcgcga gaaccacaac ctggcggagc gggcgcggcg ggccagcgag gaggggctgg
     1801 cgatcgtgga tccggtggtg gactaccgct tgccggggga ggtgatcgcg ggggcgacga
     1861 tggatctgca ggagaagctg ctgctgatgg cgatggagcg cgcggagcga ttcacggcgg
     1921 tgacggggcg ggcgtggcgg atcggggatg tgcagttcgg ggtggggagc gagttcgacg
     1981 acgggacgtc gccgaagttg ttgcggcggg aggcggcgtt gttggcgggg ggtggggagg
     2041 atttgttgac gacgtcggag cggttgttgt tggtggcggc ggtgacgttg aaggtggggg
     2101 gtgcggtggg gtgagggggt gcggtcgcgc gcagagcgcg ctcctacagg ggggcgggtt
     2161 ctcgggatcc tggcggcggt tgcgatgacg gtggggtgtg aggtcgtgcg gaaggtgcgc
     2221 gacagcgacg gcgcggcttc gcgtgtagga gcgcatctca tgcgcgaccg atgtcgccgc
     2281 gccgcccgtt gcggcgttga ggccgatcgc tacttcggct tgcgggcgct ggccgtttcg
     2341 gcgtggcggc gccccaggcg cagcacgccg ctcgggtcga ccacgatcag gccggtatcg
     2401 cgcaggtggc gcatgtcgcg ctggcgggtc ttttcggtct tgcggaagta gaggccctgg
     2461 tagatcgggt cgacgcgcag ggcttcgagg gtgagcgggc cgggatggtc gaccagcttg
     2521 cgcaggatgg cgtactggcg tgggttgatc agcttctcgt tccaggcacc gagggcctgg
     2581 ttcttcagca gcagttcgtc gaccagctcg ttcacgcggc cctgtaggcg ggcgatgacc
     2641 acgcgcaggc cttcgaggtg gaacaacacg aatggctggt tcggatgggg ctggtcggtt
     2701 gcagcggcct tgcggcaggc attgaacagc gcgaagtacg ggtgcagctg ctcctggtag
     2761 tacttggcca tggcgaatgg cgcgtacttg tagcccgcgt gccgcaggat ggtggcctcg
     2821 agcacgcggc cgacgcggcc attgccgtcc cagaaggggt ggatcaactc gtagtagagg
     2881 tgcaccagcg gtgcacgcac gaggggcgag atgccggcgt cgacgagctg gcggtgccag
     2941 tccatcagcg cgtccatcaa ccgatcgatg tcgccgcgga actgcggtgg tttgtactgg
     3001 ccgccgtggt cggcatcgcc gaccaccgtg ggtcgctcgc ggggattgtc gcggaacagg
     3061 cccggccgat tgtccgggtg cggcaggtcc gcgcagatgc gggcgtggat ggcacggacg
     3121 tagtcgacgc tcagttgcca gccggggttt tccgcggcgg cgacggcgtg gtcgtacgcg
     3181 gccttgatgt tgcgcacccg gagctgttgc tcgccctgca ccttgtccgg gtcgagatcg
     3241 agggcgcttt gcgtctggtc cagggtgagc gtcgcgcctt cgatggtgtt ggtaccgaag
     3301 acggactgca ccaggacttc gcgctggagc tggttggcca cttcggccag cggcgaggca
     3361 cggaaggtct gccatgcgtc gtcgacccgg atggtgggcg tttggagcgc gatggggtcg
     3421 acgccgagct ggaaggtgaa agggccggcg cggctcgtct ggaccttcac ggggggcagt
     3481 ggggtgatgg gcatgtcgaa catcttgtcg gacatgatgt cgccagtcaa atgcagattg
     3541 aaccaaggaa aatcaattga ttaagtagca atgcacgatg aatttgtcgc atgaaatgtc
     3601 gcgcctggct cgacgatttg ttgggtaggg ccgtggcgct tgcatgaggg cttgtcgagg
     3661 ctgacacata gcctttaggg tatgatgcat cgcatgtccg atagttcata ggctatttgc
     3721 catgacccag acacctgcga tggtgcggac gtcgctggac cagcgcctgg ccccggtgcg
     3781 gattcaggcg gacgccttgg ccgctccgcg taccggatgg atccgcgcga tccgcgaggc
     3841 gctggggatg agcgcgacgc aattggcgcg gcggatgggc gtggcgcagc cgacgctgac
     3901 gaagctcgag caatccgaag ccgccgaccg gatccagctc gacagcctgc ggcgggtggc
     3961 ggcggcgctc gattgcgacg ttgtctacgc gctggtgccg cggcggccgt tgcaggagat
     4021 ggtggaggcg cggcgggtgc tggtggccgg gcgcgagtac gcgcgggcgg cgcatagcat
     4081 cgcgctggag gatcgggcag atgacgagcc tggggtgaag gcgctgcacg acgcggcgat
     4141 gcgcgaggtg gtgggggatc gggagttgtg gagcgagcgg tgagggttgc cgacgttagt
     4201 ggtgtgagcg ctcgcttgtg ggagcggctt cagccgcgac caggcctatc gcgcgcagag
     4261 cgcgctccta cagag
//
LOCUS       DAORUK010000169         4238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_26669_length_4238_cov_19.060483, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000169 DAORUK010000000
VERSION     DAORUK010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26669_length_4238_cov_19.060483"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..597)
                     /locus_tag="PK818_09495"
     CDS             complement(40..597)
                     /locus_tag="PK818_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HQY83002.1"
                     /translation="MTDAFDPARVEADVRRWLERAVIGLNLCPFAKAVYARNQVRIVV
                     SDASTERALLEQLGEEMALLRDTPAGQVDTTLLVHPQVLGDFLDFNDFLDDADALVEA
                     LDLDGVLQVASFHPHYQFAGTEPDDAVNLTNHAPYPILHLLREDSVDRAVAAYPEPDS
                     IIERNVAAMQELGSEGFGKLLSDEA"
     gene            complement(590..1072)
                     /locus_tag="PK818_09500"
     CDS             complement(590..1072)
                     /locus_tag="PK818_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HQY83003.1"
                     /translation="MPSFDIVSEVDTHELTNAVDQANRELSTRFDFKGVEASFVLDDN
                     VISQSAPSEFQLQQMTDILRARLVARKIDARCLEFGDIDTNLAGARQKVTVKQGIERE
                     LAKKLQAAMKDAKLKVDSQINGDKLRVSGKKRDDLQAAMALLRAQEFERPLQFDNFRD
                     "
     gene            1177..2028
                     /locus_tag="PK818_09505"
     CDS             1177..2028
                     /locus_tag="PK818_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011348672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQY83004.1"
                     /translation="MLMMLAAVGMFALMDAALKLLSAHYPPFQVAALRGLSSLPLVLA
                     WALATVGPAALLRVRWPLHLMRGVLGITMMASFVFALKRLPLSTAYSIFFVAPLLITA
                     LSVPILGERVGPRRWTAIAIGLLGVLVVLRPTGEGVFTLAGLAVLLAATGYAVSAITV
                     RVLARTDSNQAMVVWLLALMALGAGALALPQWVPLRMQDAWLIAGIGVAGSIGQYTIT
                     EAFRLGEASLIAPLEYTALVWGVALDLALWGVLPDSVTWLGAAIIIASGLYLIRREKV
                     HAEAEHP"
     gene            2106..2969
                     /locus_tag="PK818_09510"
     CDS             2106..2969
                     /locus_tag="PK818_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83005.1"
                     /translation="MQRTPLIVAIVASLGLAGALAYAANSGAITRVSAPSARASLQTL
                     QSAPAGRAFAAAQSTRSAKPAPPPPPPPTVEDVGDVDSFGRNLRWLGVASGFAVVSTD
                     CASILAQDPDATCQQVSSQPGVTSFTFNDVARIRLPAGASHSLLCHWFSPQVTASFTN
                     SSDAPVLARLTSRPSVTVENPVLNDPALIDPTTGAAFGGKLTTSVTGTERLVFTLAPW
                     AQHHQTLRGSQTCIGGMLSKRALVEGYGLTEAQAASFFASPTTLRLNIQGVTASADEV
                     TFSVGLRVIGD"
     gene            complement(3034..3255)
                     /locus_tag="PK818_09515"
     CDS             complement(3034..3255)
                     /locus_tag="PK818_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83006.1"
                     /translation="MKSIQHVLWRGALALGLMVVGLGSGLGMPGTGGTAATDCRITPQ
                     VSLAGQRHPGLARGYAVLVVLPPPRKTTG"
     gene            3496..>4238
                     /locus_tag="PK818_09520"
     CDS             3496..>4238
                     /locus_tag="PK818_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HQY83007.1"
                     /translation="MAELKEARVPDIGGHGDVPVIELLVKPGDTVARDQGLVTLESDK
                     ATMEVPSPFAGVVRELKVKLGDTLSEGGLVAIIEIAAEATGAAVAAAPTPAPPPPPGA
                     QPEAVRPQAAPAEAAAPVAPVAVAATPGTIAGASAASPVAPMGAGLPPVRFDADAVLP
                     DRVPYASPAVRLFARELGVDLMQVTGSERGGRIGKDDVRRFVKAALAGGIAAPVAAAA
                     PAGGGGLNLIPWPTVDFAKFGAIESKPLSR"
BASE COUNT          579 a         1483 c         1489 g          687 t
ORIGIN      
        1 ccgcagtaac gcctcgacat cggctccgct ttggcggttt caggcttcgt cggacagcag
       61 cttgccgaaa ccttcgcttc cgagttcctg catcgccgcg acattgcgct cgatgatgct
      121 gtccggttcc ggatacgcgg ccaccgcgcg gtccacgctg tcctcgcgca acaggtgcag
      181 gatcgggtac ggcgcgtggt tggtcaggtt cacggcatcg tccggctcgg tgccggcgaa
      241 ctggtagtgc ggatggaaac tcgcgacctg cagcacgcca tccaggtcca gcgcctcgac
      301 cagggcatcg gcgtcgtcca ggaaatcgtt gaagtccagg aaatccccca gcacctgcgg
      361 atgcaccagc agggtggtgt cgacctgccc ggcgggcgtg tcgcgcagca gggccatctc
      421 ctcgccgagt tgctccagca gcgcgcgttc ggtgctggcg tcgctgacca cgatccgaac
      481 ctggttcctg gcgtacaccg ccttggcgaa cgggcacagg ttcagcccga tgaccgcgcg
      541 ctccagccag cggcggacgt cggcctcgac ccgggccggg tcgaacgcgt cagtcacgga
      601 agttgtcgaa ctgcagcggg cgctcgaact cctgcgcgcg cagcaaggcc atggcggcct
      661 gcaggtcgtc gcgcttcttg ccgctgaccc gcagcttgtc gccgttgatc tgcgagtcga
      721 ccttgagctt ggcgtccttc atcgcggcct gcagcttctt cgccagctcg cgctcgatgc
      781 cctgcttcac cgtcaccttc tgccgcgcgc cggccaggtt ggtgtcgatg tcgccgaact
      841 ccaggcagcg cgcatcgatc ttccgcgcca ccagccgcgc acgcaggatg tcggtcatct
      901 gctgcagctg gaactcgctg ggcgcggatt gcgagatcac gttgtcgtcg agcacgaacg
      961 acgcctccac gcccttgaag tcgaagcgcg tcgacagctc gcggttggcc tggtccaccg
     1021 cgttggtgag ttcgtgggta tccacttcgg aaacgatgtc gaaggaaggc atggcgcagg
     1081 ccggcaggtg acggggcggg caggttaccg catgctggcg cgatggaaac gctgaccacc
     1141 gaaatgcgcg cgcaggcgca acgccggcac cgcggcatgc tgatgatgct ggcggcggtc
     1201 ggcatgttcg cgctgatgga tgccgcgctc aagctgctgt cggcgcacta tccgccgttc
     1261 caggtggcgg ccctgcgcgg gctgtcctcg ctgccgctgg tgctggcctg ggcattggcg
     1321 acggtcggtc cggcggcgct gctgcgcgtg cgctggccgc tgcacctgat gcgcggcgtg
     1381 ctcgggatca cgatgatggc cagcttcgtc ttcgcgctga agcggctgcc gctgtccacc
     1441 gcgtattcga tcttcttcgt cgccccgctg ctgatcaccg cgctgtcggt gccgatcctc
     1501 ggcgagcggg tcgggccgcg gcgctggacc gcgatcgcga tcggcctgct cggcgtgctg
     1561 gtggtgctgc gcccgaccgg cgagggcgtg ttcacgctgg cgggcctggc ggtgctgctg
     1621 gccgccaccg gctacgcggt ctcggcgatc accgtgcggg tgctggcgcg gaccgattcc
     1681 aaccaggcga tggtggtatg gctgctggcg ctgatggcgc tcggcgccgg cgccctggcg
     1741 ctgccgcagt gggtgccgct gcggatgcag gatgcctggc tgatcgccgg gatcggggtt
     1801 gccggttcga tcggccagta caccatcacc gaagcattcc ggctcggcga ggcgtcgctg
     1861 atcgcgccgc tggaatacac cgcgctggtg tggggggtgg cattggacct ggcgctgtgg
     1921 ggcgtgctgc cggactcggt cacctggctg ggcgcggcga tcatcatcgc cagtggcctg
     1981 tacctgatcc gccgcgaaaa ggtgcacgcg gaggcggagc acccctgaag cgggcggcgt
     2041 gccggcgatg tccggtttcg gcgcgctgct gcgcatacac caggacatcc accaggagtc
     2101 ctgccatgca acgcacgcca ctgatcgtcg ccatcgtcgc cagcctcggc ctggccggcg
     2161 cccttgccta tgccgccaac agcggtgcca tcacgcgggt atccgcgcca tcggcgcgtg
     2221 cgtcactgca aacgctgcag tccgcaccgg ccggccgggc gttcgccgcc gcccagtcga
     2281 cccggtctgc gaagccggcg ccgccaccgc cgccgccgcc gaccgtcgaa gacgtgggcg
     2341 acgtcgattc cttcggccgc aacctgcgct ggctcggcgt cgccagtggc ttcgcggtgg
     2401 tctccaccga ctgcgcgtcg atccttgccc aggacccgga tgccacctgc cagcaggtca
     2461 gcagccagcc cggcgtcacc agcttcacct tcaatgacgt cgcccgcatc cggctgcccg
     2521 cgggcgcctc ccactcgctg ctctgccact ggttctcgcc gcaggtgacc gcgtcgttca
     2581 ccaattcctc ggacgcaccc gtgctggcac ggttgaccag ccgtcccagc gtgaccgtgg
     2641 agaatccggt gctgaacgat ccggcgttga tcgatccgac caccggggcg gcgttcggcg
     2701 gcaagctcac caccagcgtc accggcaccg agcgcctggt cttcacgctg gcgccgtggg
     2761 cccagcacca ccagaccctg cgtggctcgc agacctgcat cggcgggatg ctgtcgaagc
     2821 gggcgctggt cgagggctat ggcctgaccg aggcccaggc cgccagcttc ttcgccagcc
     2881 ccaccaccct gcgcctgaac atccagggtg tgaccgcctc cgccgacgaa gtgacgttca
     2941 gcgttggcct gcgggtgatc ggcgactgat cccggccacg gccggcgcgg cagcatcccg
     3001 cgccggccca tgccgattgc cgcgtgccgt cgcctagccg gtggtctttc gtggcggcgg
     3061 cagcaccacc aggaccgcgt acccgcgggc aaggccgggg tgccgctggc cggccaacga
     3121 aacctgcggc gtgatgcggc aatcggtggc ggcggtcccg ccggtgcccg gcatgcccag
     3181 gccgctgccc aggccgacca ccatcaggcc cagcgccagc gcgccgcgcc acaacacatg
     3241 ctggatcgat ttcatcgtcg ttcctcgtgg tggcgatcgg gggacatgca cgccactgca
     3301 cgagagatgc acccggcgcg cggatggttg cagcgcccga ggccggcgtg acctgcggaa
     3361 tatcggccgc cgccacgtgt cctgcgggac attcgaaaga attacccgga tgaatcacgg
     3421 aatattgccc ggcggtttgt tccggggcgc tggatgtggc aacaataccg gcctttccgg
     3481 gtcccggagt gaggcatggc tgagctgaag gaagcgcgcg tccccgatat cggcggtcat
     3541 ggcgacgtgc cggtgatcga attgctggtc aagcccggcg acaccgtggc cagggaccag
     3601 ggcctggtca ccctggagtc ggacaaggcg acgatggaag tgccgtcgcc gtttgccggc
     3661 gtggtcaggg agctgaaggt gaagctcggc gacacccttt cggagggtgg actggtcgcg
     3721 atcatcgaga tcgccgcgga ggccactggc gccgccgttg ccgcggcccc gacgccggcc
     3781 ccgccgccgc cgccgggtgc gcagcccgaa gcagtgcggc cgcaggcagc ccccgcggaa
     3841 gccgccgccc cggtggcgcc ggtggcggtc gcggcaaccc ccggcaccat cgccggcgca
     3901 tcggccgcga gcccggtcgc gccgatgggg gccggcctgc cgccggtgcg cttcgatgcc
     3961 gacgcggtgc tgccggacag ggtgccctat gccagcccgg cggtgcgcct gttcgcgcgc
     4021 gagctgggcg tggacctgat gcaggtcacc ggcagcgaac gcggcggccg catcggcaag
     4081 gatgacgtgc gcaggttcgt gaaggcggcg ctggcaggcg ggattgccgc gccggtcgcg
     4141 gcggccgcac ccgccggtgg cggcggcctc aacctgatcc cgtggccgac ggtggatttc
     4201 gcgaagttcg gcgccatcga aagcaagccg ctgtcgcg
//
LOCUS       DAORUK010000170         4236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_26687_length_4236_cov_19.150442, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000170 DAORUK010000000
VERSION     DAORUK010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26687_length_4236_cov_19.150442"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PK818_09525"
     CDS             complement(<1..917)
                     /locus_tag="PK818_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014508703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQY83008.1"
                     /translation="MATIDKPASRNALIVDDERDIRELLVLTLGRMGLRCDTAGSLND
                     ARALLKANRYALCLTDMRLADGSGVDLVREIAQLYAETPVAMITAFGNQDAAVGALKA
                     GAFDFVSKPVDLAVLRGLVQHALKLGEEGRKGRADAADPVAARLLGRSRAMEELRATL
                     SKVARSQAPVYIAGESGVGKELVARTIHAEGARAGGPFVPVNCGAIPAELMESEFFGH
                     KKGSFTGAHVDKPGLFQAAEGGTLFLDEVAELPLPMQVKLLRAIQEKSVRPVGAQNEV
                     AVDVRILSATHKNLAALVEDGRFRHDLYYR"
     gene            complement(971..2581)
                     /locus_tag="PK818_09530"
     CDS             complement(971..2581)
                     /locus_tag="PK818_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HQY83009.1"
                     /translation="MPARPVDTADPATPQSAIRRELYLLTLYRVLEAALLCLAVFSPV
                     GSMIGEPRHLPLGRVVALAYLAVAIALFVGGRRGNIRSQALLGAATDILAAGLAMHAL
                     PASAAGIAMMLLFNIGATAFLLPLRLGIGCAVLAALALAAEFVASVLHDGGARTPLAE
                     LTMFAVSYLAMATLTNLVGRQMRATEALAERRGTQAANLAEVNELIIRRMRTGVLLVD
                     GDGTIKLANEAAMLQLGKLDEGRRDLALAAPALARRLQEWRRDGGNDAQPLQMAAGLP
                     EVLPRFARLLAEGDQALVFLDDTSQVTRRAESLTLATLGRFSASLAHEIRNPLAAISY
                     ATQLLEESREIPEADRRLLEIIYQQTQRMNGIVENVLGLARRERAQPEHVELGEFIRH
                     FVEDYRNSHPLEVDRLDALAPTAPLVAAVDRRQLQQALTVLVHNALVYGRLPGEAARI
                     SIRAYADDAGTPVLDVVDRGPGIPETVAAQLFRPFFTTSGHGTGLGLYIARELCQANE
                     ASLDYVPVPAGGACFRIRLSGARAINTT"
     gene            2712..3878
                     /gene="sucC"
                     /locus_tag="PK818_09535"
     CDS             2712..3878
                     /gene="sucC"
                     /locus_tag="PK818_09535"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HQY83010.1"
                     /translation="MNFHEYQAKQLFADYGIAVPAGRVARSPEEAVDAAKAIGGEAWM
                     VKAQIHAGGRGKAGGVKYRKSFDEVREYAKGMLGTKMETYQSAGVALPVDSVLVTQAT
                     DIAQELYLSVMVDRASQAITFIASADGGVEIEKTAVENPDAIKTLVVNYVQGLQPYQT
                     RDMGFALGLNARQVNQLGKIMLGLFRLFNEKDLALVELNPLAILTDGNLAVLDGKINS
                     DDNANFRHPELVEMRDIKQEDETEVLASKYDLNYVTMDGNIGCMVNGAGLAMATMDVI
                     KLNGGEPANFLDVGGGANKEKVTEAFKLILRSPDVKAIFVNIFGGIVRCDMIAEGIIA
                     AVKEVGVKVPVIVRLEGTNVDAGKELLKNSGLAITSADDINDGAKKAVAAAKAA"
     gene            3975..>4236
                     /locus_tag="PK818_09540"
     CDS             3975..>4236
                     /locus_tag="PK818_09540"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971492.1"
                     /note="Catalyzes the only substrate-level phosphorylation
                     in the TCA cycle; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HQY83011.1"
                     /translation="MSVLINKDTKVIVQGFTGSQGTFHAEQMVDYGTQVVGGVTPGKG
                     GTQHLGLPVFNTVAESVKATGADASVIYVPPPFAADAILEAAD"
BASE COUNT          687 a         1432 c         1490 g          627 t
ORIGIN      
        1 cggtagtaca ggtcgtggcg gaagcggccg tcctcgacca gcgcggccag gttcttgtgg
       61 gtggccgaca ggatccgcac gtccaccgcg acttcgttct gggcgccaac cgggcgcacc
      121 gacttttcct ggatcgcgcg cagcagcttg acctgcatcg gcagcggcag ttcggcgact
      181 tcgtccagga acagggtgcc gccctcggcc gcctggaaca ggcccggctt gtccacgtgg
      241 gcgccggtga agctgccctt cttgtggccg aagaactcgc tttccatcag ttcggccggg
      301 atcgcgccgc agttgaccgg cacgaacggg ccgccggcgc gcgcgccctc ggcgtggatg
      361 gtgcgggcga ccagttcctt gcccacgccg gactcgccgg cgatgtacac cggggcctgg
      421 ctgcgcgcga ccttggacag ggtggcgcgc agttcctcca tcgcccgtga cctacccaga
      481 aggcgggcgg caaccgggtc ggctgcgtcg gcgcggccct tgcggccttc ctcgcccagc
      541 ttcagcgcgt gctggaccag cccgcgcagc accgcaaggt cgaccggctt gctgacgaaa
      601 tcgaaggcgc cggccttgag cgcgccgacc gccgcgtcct ggttgccgaa cgcggtgatc
      661 atcgccaccg gggtttccgc atacagctgg gcgatctcgc gcacgaggtc cacgccggag
      721 ccgtcggcca ggcgcatgtc ggtcaggcac agggcgtagc ggttggcctt gagcagggcg
      781 cgcgcgtcgt tcagcgagcc ggcggtatcg cagcgcaggc ccatgcggcc aagggtgagg
      841 accagcagtt cgcggatgtc gcgttcgtcg tcgacgatca gcgcgttgcg cgaggccggt
      901 ttgtcaatag ttgccattgc gcgtcacatt acccggttcg ccccgcgctg tgccagtgga
      961 aaaaggggcc tcaggtggtg ttgatggccc gcgcgccgga cagccggatc cggaagcagg
     1021 cgccgccggc cgggaccggc acgtagtcga ggctggcctc gttggcctgg cacagttcgc
     1081 gggcgatgta gaggcccagc ccggtgccgt gcccggaggt cgtgaagaac ggccggaaca
     1141 gctgcgccgc caccgtttcc gggatgccgg ggccgcggtc gaccacgtcc agcaccgggg
     1201 tgccggcgtc atccgcatac gcacggatac tgatccgcgc ggcctcgccg ggcagccgcc
     1261 cgtataccag cgcgttgtgg accagcaccg tcagcgcctg ctgcagctgg cggcggtcga
     1321 ccgccgccac cagcggggcg gtcggggcca gcgcatccag gcggtcgact tccagcgggt
     1381 ggctgttgcg gtagtcctcc acgaaatggc ggatgaactc gcccagctcc acgtgttccg
     1441 gttgcgcgcg ctcgcgccgg gccaggccca gcacgttctc gacgatgccg ttcatgcgct
     1501 gggtctgctg gtagatgatc tccagcaggc ggcggtcggc ctcggggatc tcgcgcgatt
     1561 cctccagcag ctgggtggcg tagctgatcg cagcgagcgg attgcggatc tcgtgggcga
     1621 ggctggcgga aaaccggccg agggtggcca gggtcagcga ttcggcccgc cgcgtgacct
     1681 gcgaggtatc gtcgaggaac accagcgcct ggtcgccttc ggccagcagg cgggcgaagc
     1741 gcggcagcac ctccggcagg ccggccgcca tctgcagcgg ttgcgcgtcg ttgccgccgt
     1801 cgcggcgcca ttcctgcagg cgccgggcca gcgccggcgc ggccagcgcg aggtcgcggc
     1861 ggccctcgtc cagcttgccc agttgcagca tcgccgcctc gttggccagc ttgatggtgc
     1921 cgtcgccgtc caccagcagg accccggtgc gcatccggcg gatgatcagt tcgttgactt
     1981 cggccaggtt cgcggcctgg gtgccgcggc gttcggccag cgcctcggtg gcgcgcatct
     2041 ggcggccgac caggttggtc agggtggcca tcgccaggta gctgaccgcg aacatggtca
     2101 gctccgccag cggggtgcgg gcgccgccgt cgtgcagcac gctggccacg aattccgcgg
     2161 ccagcgccag cgccgccagc accgcgcagc cgatccccag ccgcagcggc agcaggaacg
     2221 cggtggcgcc gatgttgaac agcagcatca tggcgatgcc ggcggcggac gccggcagcg
     2281 cgtgcatggc caggccagcg gcgaggatgt cggtggcggc gcccagcagg gcctgggagc
     2341 ggatgttccc gcgccggccg ccaacgaaca gcgcgatggc cacggccagg taggccagcg
     2401 ccaccacccg ccccagcggc aggtggcgcg gttcgccgat catgctcccg accgggctga
     2461 acacggccag gcacagcagc gccgcctcga ggacccggta cagggtcagc aggtacagct
     2521 cgcgccggat ggcggactgc ggggtggccg ggtcggcggt gtcgacgggg cgggcgggca
     2581 tggcccgagt atgcgcgaac cccgcccggc tggcgcaaca ggctaaaatg acggttccgc
     2641 ccgcgccctg ccggaccccg gcccgcggtg cgtccctgcc ttcaacggca actctctccc
     2701 ctgggtgatc catgaatttc cacgaatatc aggcgaaaca gctgtttgcc gattacggca
     2761 tcgcggtacc ggccgggcgc gtcgcgcgtt cgcccgaaga ggccgtcgat gcggccaagg
     2821 ccatcggtgg cgaggcctgg atggtcaagg cgcagatcca cgcgggcggc cgcggcaagg
     2881 ccggcggcgt gaagtacagg aagtcgttcg atgaagtgcg cgagtacgcc aagggcatgc
     2941 tgggcacgaa gatggaaacc taccaatcgg cgggcgtggc gctgccggtg gattcggtgc
     3001 tggtgaccca ggccaccgac atcgcccagg agctgtacct gtcggtgatg gtggaccgtg
     3061 ccagccaggc gatcaccttc atcgcctccg ccgacggcgg cgtcgagatc gagaagaccg
     3121 cggtcgagaa ccccgacgcg atcaagaccc tggtggtgaa ctacgtgcag ggcctgcagc
     3181 cttaccagac ccgcgacatg ggcttcgcgc tgggcctgaa cgccaggcag gtgaaccagc
     3241 tcggcaagat catgctgggc ctgttcaggc tgttcaacga gaaggacctg gcgctggtcg
     3301 aactgaaccc gctggcgatc ctcaccgacg gcaacctggc cgtgctcgac ggcaagatca
     3361 attccgacga caacgccaac ttccgccacc ccgaactggt ggagatgcgc gacatcaagc
     3421 aggaagacga gaccgaagtg ctggccagca agtacgacct caactacgtg accatggacg
     3481 gcaacatcgg ctgcatggtc aacggtgcgg gcctcgccat ggcgaccatg gacgtgatca
     3541 agttgaacgg cggcgagccg gccaacttcc tcgacgtcgg cggcggcgcc aacaaggaga
     3601 aggtcaccga ggcgttcaag ctgatcctgc gctcgccgga cgtgaaggcg atcttcgtca
     3661 acatcttcgg cggcatcgtc cgctgcgaca tgatcgccga gggcatcatc gccgcggtca
     3721 aggaagtggg cgtcaaggtg ccggtgatcg ttcgcctgga aggcaccaac gtcgatgccg
     3781 gcaaggagct gctgaagaac tccggcctgg cgatcacgtc ggccgacgac atcaacgacg
     3841 gcgcgaagaa agccgtcgcc gctgccaagg ccgcgtaatc ccatatcacc tgtaggagcg
     3901 gcgtaagccg cgacctgcca atcaacggtc gcggcttacg ccgctcctac agaagcattt
     3961 tcgaaggatt cccaatgtcc gttctgatca acaaagacac caaggtcatc gtgcagggct
     4021 tcaccggctc gcagggcacc ttccacgccg agcagatggt cgactacggc acccaggtcg
     4081 tcggcggcgt caccccgggc aagggcggca cccagcacct tggcctgccg gtgttcaaca
     4141 ccgtcgccga gtcggtcaag gccaccggcg ccgatgcgtc cgtcatctac gtgccgccgc
     4201 cgttcgcggc cgacgcgatc ctggaagcgg ccgatg
//
LOCUS       DAORUK010000171         4236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_26688_length_4236_cov_17.025353, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000171 DAORUK010000000
VERSION     DAORUK010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26688_length_4236_cov_17.025353"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PK818_09545"
     CDS             complement(<1..898)
                     /locus_tag="PK818_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HQY83012.1"
                     /translation="MSNATPTLVIAITARALFHLEDSHGLFEAKGVKAYADYQRQRED
                     DMLEPGIAFPLVRKLLALNEGSPADAPRVEVILLSRNSSDTGLRIFNSIQHHRLDIRR
                     ATFTSGAPVWPYIRPFGAQLFLSANPESVRRALEAGIAAATILPARAPESAPGDARHD
                     QVRIAFDGDAVIFGDESERVSREQGVEAFGRHERERAREPLSGGPFRGFLSALHDLQA
                     AFPAGDDAPIRTALVTARSAPAHERVIRTLREWEVRLDEALFLGGRDKGPFLEAFGAD
                     IFFDDSPHNIDSARRHVAAGHVP"
     gene            complement(957..1739)
                     /locus_tag="PK818_09550"
     CDS             complement(957..1739)
                     /locus_tag="PK818_09550"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HQY83013.1"
                     /translation="MSMPRLAFLASPTDDAQAALKALTSLHGVVTLQDADVLVALGGD
                     GFMLQTLHRHGALGKPVYGMKLGTVGFLMNHGSPGSGPVQALQQRIAQAEPAVLHPLE
                     MLAQTESGATVGSLAYNEVSLLRQTRQAAHVGIELNGQLRLDELICDGVMVATPAGST
                     AYNFSANGPILPLGANVMALTPIAPFRPRRWRGAVLKSGTEVRFRVLDPHKRPVSATA
                     DSHEVRDVTEVLVREQRERSVTLLFDPEHNLEERILAEQFTS"
     gene            complement(1865..>4236)
                     /locus_tag="PK818_09555"
     CDS             complement(1865..>4236)
                     /locus_tag="PK818_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HQY83014.1"
                     /translation="DFRTEVLGLVKAQMVKNTVIVPVGSKGGFFCKQSPDPASNRDAW
                     FNEGVACYKRFINGLLDITDNLAPDGKVLPPAGVVRHDGDDPYLVVAADKGTATFSDI
                     ANGIAQAHGFWLDDAFASGGSVGYDHKGMGITARGAWESVMRHFRAMGRDSQKQEFTC
                     TGIGDMSGDVFGNGMLLSKQIRLLAAFDHRHVFIDPNPDAAASFKERARMFKLPRSSW
                     EDYDKALISKGGGVWPRSAKSIPLSAEARTALGIEGDVASMSPMELMNAILRAPVDLL
                     WNGGIGTYVKSSRENNADVGDRANNGLRVDGNQLRCKVVGEGGNLGLTQLGRIEAAHH
                     GVLLNTDFIDNSAGVDTSDHEVNIKILLNGEVQKKKLTLPERNKLLASMTAEVADLVL
                     NDNYRQNQAISLMERMSLSRLGSKQHFIRTLEAQGLLDRQIEFLPSDAEIAERKARGL
                     GLTRPELSVLLSYSKLVAFQQLLESDVPEDPYLSKELERYFPQPLQAKYAKAMQQHRL
                     KREIIATAVTNSTINRMGATFLLRMQEDSGRTPGEVAKAFTITRETLDARELWTKIDA
                     LDGKVAESVQIDALQVIWNLQRSFTRWLLSRAGAIPDIATAVARYHDGFRDIRAGEGI
                     LPSAQRPAYEASLRNWRAHGVPQELAMQIAALPYLEPCADIIEVARERKLKPVDVAKV
                     HFRIGDALNLPWLQEQIDSLPVDGRWHAVARGVLREELATQQRALVQQVLARPGANAD
                     AKVRSWLERDDAALRFTLSMLNELAAQKTLDYPTASVAVQRLAQLASRS"
BASE COUNT          607 a         1438 c         1490 g          701 t
ORIGIN      
        1 gcggcacgtg gccagcggcg acgtggcggc gcgccgaatc gatgttgtgc ggcgagtcgt
       61 cgaagaagat gtcggcgccg aatgcctcca ggaacgggcc cttgtcgcgg ccgccgagga
      121 acagcgcctc gtccaggcgc acttcccatt cgcgcagggt gcggatcacg cgttcgtgcg
      181 cgggcgcgga gcgtgcggtc accagcgcgg tgcggatcgg cgcgtcatca cccgccggga
      241 acgccgcctg caggtcatgc agggcggaca ggaagccgcg aaacgggcca ccggacaacg
      301 gttcgcgggc gcgttcgcgc tcgtggcggc cgaacgcctc caccccctgc tcgcgcgaga
      361 cgcgctcgct ctcgtcgccg aagatcaccg cgtcgccgtc gaatgcgatc cgcacctggt
      421 cgtggcgcgc atcgccgggt gccgactccg gcgcacgcgc gggcaggatc gtggccgcgg
      481 cgatgcccgc ctccagcgcc cgccgcaccg attccgggtt cgcggacagg aacagctgcg
      541 cgccgaacgg ccggatgtag ggccacaccg gcgcccccga ggtgaaggtg gcgcggcgga
      601 tgtcgaggcg gtggtgctgg atcgaattga agatgcgcag gccggtgtcg gacgaattgc
      661 gcgagagcag gatgacctcg acccgcggtg cgtccgcggg cgaaccctcg ttcaaggcca
      721 gcagcttgcg caccagcggg aacgcaatcc ccggttccag catgtcgtcc tcgcgctggc
      781 gctggtagtc ggcgtaggcc ttcacgccct tggcctcgaa caggccgtgg ctgtcctcga
      841 ggtggaacag cgcacgcgcg gtgatggcga tgaccagggt cggggtggcg ttgctcatgg
      901 cggaagtatc gccgctggcg gtcgcgccgg ctgcgacagg gccgggtcgg tcggaatcag
      961 gaagtgaact gctcggcgag gatccgctct tcgaggttgt gctcggggtc gaacagcagg
     1021 gtcacgctgc gctcgcgctg ttcgcgcacc agcacctcgg tgacgtctcg cacctcgtgg
     1081 gagtcggcgg tcgcgctgac cgggcgcttg tgcggatcca gcacccggaa gcggacctcg
     1141 gtgccggact tgagcaccgc cccacgccag cgtcgcggcc ggaacggcgc gatcggggtc
     1201 agcgccatca cgttcgcgcc cagcggcagg atcggcccat tcgcggaaaa attgtaggcg
     1261 gtgctgccgg caggggtcgc caccatcacc ccgtcgcaga tcagctcgtc gagccgcagc
     1321 tggccgttga gctcgatgcc gacgtgcgcc gcctgccggg tctggcgcag cagcgacacc
     1381 tcgttgtagg ccagcgagcc gacggttgcg ccggattcgg tctgcgccag catctccagc
     1441 gggtgcagca ccgccggttc ggcctgcgcg atgcgttgct gcagcgcctg caccggaccc
     1501 gaaccggggc tgccgtggtt catcaggaag ccgaccgtgc ccaatttcat gccgtacacc
     1561 ggcttgccga gggcgccgtg ccggtgcagg gtctgcagca tgaacccgtc gccgccaagc
     1621 gcgaccagca cgtcggcatc ctgcagggtg accacgccgt gcagggaggt cagtgccttc
     1681 agcgcggcct gggcgtcatc ggtgggactg gccaggaatg cgaggcgggg catgctcatt
     1741 cgcgaagctt accccgaatg caggccctgc ccgcctgccg gcgaggctgg cacgcggggc
     1801 ttccggcgaa agcccccgcc agtggattgg tcccgtttcc cgcaggcggg agacgggacg
     1861 ccgcctagct gcgcgaagcc agctgcgcga gccgttgcac cgccaccgac gcggtcgggt
     1921 agtccagggt cttctgtgct gccagctcat tgagcatcga cagggtgaag cgcagcgcgg
     1981 catcgtcccg ctccagccag ctcctgacct tggcatcggc gttcgcaccc gggcgcgcga
     2041 gcacctgctg caccagcgcg cgctgctgcg tggccagttc ctcgcgcagc acgccgcgcg
     2101 ccaccgcatg ccagcggcca tccaccggca gcgagtcgat ctgctcctgc agccacggca
     2161 ggttgagcgc gtcgccgatc cggaagtgca ccttggccac gtccaccggc ttgagcttgc
     2221 gttcgcgcgc gacctcgatg atgtccgcgc acggctccag gtacggcagc gcggcgatct
     2281 gcatcgccag ttcctgcggc acgccatgcg cgcgccagtt gcgcaggctg gcctcgtagg
     2341 ccgggcgctg cgccgaaggc aggatgccct cgcctgcgcg gatgtcgcgg aagccgtcgt
     2401 ggtagcgcgc caccgcggtg gcgatgtcgg ggatcgcgcc ggcgcgcgac agcagccagc
     2461 gggtgaagct gcgctgcagg ttccagatca cctgcagggc gtcgatctgc accgactcgg
     2521 cgaccttgcc gtccagcgcg tcgatcttcg tccacagctc gcgcgcgtcc agggtctcgc
     2581 gggtgatggt gaaggccttg gcgacttcgc ccggggtgcg gccgctgtct tcctgcatgc
     2641 gcagcaggaa ggtggcaccc atccggttga tggtggaatt ggtcaccgcg gtggcgatga
     2701 tttcgcgctt caggcggtgc tgctgcatcg ccttcgcgta cttcgcctgc aacggctgcg
     2761 ggaagtagcg ctcgagttcc ttgctcaggt acgggtcttc cggcacgtcg gattccagca
     2821 gttgctggaa cgcgaccagc ttggaatacg acagcagcac cgacagctcc gggcgggtca
     2881 ggcccaggcc acgtgccttg cgctcggcga tctccgcgtc ggacggcagg aactcgatct
     2941 ggcggtccag caggccctgc gcttccaggg tgcggatgaa gtgctgcttg gagccaagcc
     3001 gcgacaggct catccgctcc atcaggctga tcgcctggtt ctgccggtaa ttgtcgttga
     3061 gcaccaggtc ggcgacctcg gcggtcatcg aggccagcag cttgttgcgt tccggcaggg
     3121 tcagcttctt cttctggacc tcgccgttga gcaggatctt gatgttcacc tcgtggtcgg
     3181 aggtgtccac gccggcggag ttgtcgatga aatcggtgtt gagcagcacg ccgtggtgcg
     3241 ccgcctcgat gcgcccgagc tgggtcaggc cgaggttgcc gccctcgccc accaccttgc
     3301 agcgcagctg gttgccgtcc acccgcaggc cgttgttggc gcggtcgccg acgtcggcgt
     3361 tgttctcgcg gctggacttc acgtaggtgc cgatgccgcc gttccacagc aggtcgaccg
     3421 gtgcccgcag gatcgcgttc atcagctcca tcgggctcat cgacgccacg tcgccctcga
     3481 tcccgagcgc ggtcctggcc tcggccgaca gcgggatcga cttggcgctg cgcggccaca
     3541 cgccgccgcc cttgctgatc agggccttgt cgtagtcttc ccagctcgaa cgcggcagct
     3601 tgaacatgcg cgcgcgttcc ttgaacgagg cggccgcatc cgggttcggg tcgatgaaga
     3661 catggcggtg gtcgaacgcg gccagcaggc ggatctgctt gctcagcagc atgccgttgc
     3721 cgaacacgtc gccggacatg tcgccgatcc cggtgcaggt gaattcctgc ttctgcgaat
     3781 cgcggcccat cgcgcggaaa tggcgcatga ccgattccca cgcgccgcgc gcggtgatgc
     3841 ccatgccctt gtggtcgtag ccgaccgaac cgccggaggc gaacgcgtcg tccagccaga
     3901 acccgtgcgc ctgggcgatg ccgttggcga tgtcggagaa ggtcgcggtg cccttgtcgg
     3961 cggcgaccac caggtacgga tcgtcgccgt cgtggcggac cacgccggcg ggcggcagca
     4021 ccttgccgtc cggcgccagg ttgtcggtga tgtccagcag gccgttgatg aagcgcttgt
     4081 agcaggccac gccttcgttg aaccacgcgt cgcggttgct cgcgggatcc ggcgactgct
     4141 tgcagaagaa gccgcccttg ctgccgaccg gcacgatcac ggtgttcttc accatctgcg
     4201 ccttgaccag gcccagcacc tcggtgcgga agtcct
//
LOCUS       DAORUK010000172         4172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_27335_length_4172_cov_17.825844, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000172 DAORUK010000000
VERSION     DAORUK010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27335_length_4172_cov_17.825844"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PK818_09560"
     CDS             <1..599
                     /locus_tag="PK818_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY83015.1"
                     /translation="GGGVSGTHAATIAVGMGAQVTVVDRSADALKRLASQFGTSISTV
                     FSTRSAIEELVRRADLLIGTVLVPGAAAPKLVTRDMLKTMKPGSVIVDVAIDQGGCVE
                     TSHATTHADPTYVVDGVVHYCVANMPGAVARTSTFALNSVTLPFTLALADKGWRKALE
                     QDPHLREGLNVCEGKLTCEPVAEAHGLPYTSAESVLGL"
     gene            705..1223
                     /locus_tag="PK818_09565"
     CDS             705..1223
                     /locus_tag="PK818_09565"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HQY83016.1"
                     /translation="MTDWTRPIRDVADFPKPGIVFKDIMPMLADPVAFAGAIEAMAAP
                     WRDTEIDAVLAIEARGFLFGAPLARVLGAGIVPVRKPGKLPGRVLEQRYALEYGEDAL
                     QVQADAVSPGTRVLLVDDVLATGGTLAATRLLAERLEAEVVGAEVLIELGFLNGRARW
                     NGSAPLHAALLV"
     gene            complement(1242..1580)
                     /locus_tag="PK818_09570"
     CDS             complement(1242..1580)
                     /locus_tag="PK818_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003489493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HQY83017.1"
                     /translation="MSTLPPCPQCASAYAYEDGAMLVCPECGHEWSPLQADAVDDAKV
                     VRDAVGNVLQDGDTVTVIKDLKLKGSSLVVKVGTKVRNIRLVDGDHDIDCKVDGIGQM
                     GLKSEFVKKA"
     gene            1732..>2740
                     /gene="ltaE"
                     /locus_tag="PK818_09575"
     CDS             1732..>2740
                     /gene="ltaE"
                     /locus_tag="PK818_09575"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HQY83018.1"
                     /translation="MHVIDLRSDTVTQPTRAMRAAMLEARVGDDVYGEDPTVNALQER
                     LAAELGFDAGLFVPTGTQSNLLALLAHCERGDEYLVGMEAHTYKYEGGGAAVLGSIQP
                     QPIVQATDGSLPLDSIAAAIKPVDPHFARTRLLALENTWHGRALPLDYLRSAHALARA
                     HGLGMHLDGARLFNAALACQVPPREIARHFDTVSVCLSKGLGAPVGSVLVGSRALIDK
                     ARRWRKVCGGGWRQAGLLAAAALHALDHHVARLAEDHARAAMLAERLRGIGGIDLLGQ
                     HTNMVFIDVPAERLQALDAHLRTAGIRASIGYLPTLRLVTHLDIDDDGIERTAAAFAR
                     FF"
     assembly_gap    2741..2840
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2845..3387)
                     /locus_tag="PK818_09580"
     CDS             complement(2845..3387)
                     /locus_tag="PK818_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014502872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HQY83019.1"
                     /translation="MIRSLLLAIALAGGTADARDAALPNASVPAMDLQRYSGEWHEIA
                     RLPMFFQRNCVANITATYTLRADGLVGVRNACDDKDGGRMLSEGTARPVAGHPGRLQV
                     RFAPDWLAWAPMAWADYWVVDLDPDYRWAVVGGPSRKYLWILSRTPSMDRAQYDGIRA
                     RAARRGYPVDELVLPRGTLD"
     gene            3556..4170
                     /locus_tag="PK818_09585"
     CDS             3556..4170
                     /locus_tag="PK818_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HQY83020.1"
                     /translation="MKSTLPLAIASALALVACGASEPAAADPAPAPMETAAEPTPAEP
                     VAATASTAPDAANDIVTTASATPSLSTLVAAVKAAELVATLQGAGPFTVFAPTNDAFG
                     KLPAGAVDGLLKPEMKADLAGVLTYHVVSGNVDAATLLGQIEAGGGTATLTTVQGGTL
                     TATPDGAGGITLTDAKGGTAKVTTADLRQSNGIVHVIDSVLMPK"
BASE COUNT          609 a         1467 c         1406 g          590 t
ORIGIN      
        1 tcggcggcgg cgtgtccggc acccatgcgg cgacgatcgc cgtcggcatg ggcgcgcagg
       61 tcaccgtggt cgaccgctcg gcggatgcgc tgaagcgcct cgccagccag ttcggcacct
      121 cgatctccac cgtgttctcc acccgctcgg cgatcgagga gctggtgcgt cgcgccgacc
      181 tgctgatcgg caccgtgctg gtcccgggcg ccgccgcgcc gaagctggtc acccgcgaca
      241 tgctgaagac gatgaagccg ggttcggtga tcgtggacgt cgccatcgac cagggcggct
      301 gcgtggaaac ctcgcacgcg acgacccacg ccgacccgac ctacgtcgtc gatggcgtcg
      361 tccactactg cgtggcgaac atgccgggcg cggttgcgcg cacctccacc ttcgcgctca
      421 acagcgtgac cctgccgttc accctcgcac tggccgacaa gggctggcgc aaggcactgg
      481 agcaggatcc gcacctgcgc gagggcctga acgtgtgcga aggcaagctc acctgcgaac
      541 cggtggcgga ggcgcacggc ctgccctaca ccagcgcgga gagcgtgctc ggcctgtaaa
      601 cggccaccgc gatgcagggc gaaaagggac gccatgccgt cccttttctt ttatgcacat
      661 gccgcgacgg ggcatgatgc gactttgccg aaggacgcct gccgatgacc gactggaccc
      721 gaccgatccg cgacgtggcc gatttcccga agccgggcat cgtgttcaag gacatcatgc
      781 cgatgctggc cgacccggtg gcgttcgccg gtgcgatcga ggcgatggcg gcgccgtggc
      841 gcgataccga gatcgacgcg gtgctggcga tcgaggcgcg cggcttcctg ttcggcgcac
      901 cgctggcgcg cgtgctcggc gccggcatcg tgccggtgcg caagccgggc aagctgccgg
      961 ggcgggtgct ggaacagcgt tacgcgctgg agtacggcga ggatgcgctg caggtgcagg
     1021 ccgatgcggt atcgccgggt acccgcgtgc tgctggtgga tgacgtgctg gccaccggcg
     1081 gcaccctggc ggcgacgcgg ctgctggccg aacggctgga ggccgaggtg gtcggtgccg
     1141 aggtcctgat cgaactcggc ttcctcaatg gccgcgcgcg ctggaacggc agcgcgccgc
     1201 tgcatgcggc gctgctggtc tgagcaggaa ccggagcagg ctcaggcctt cttcacgaat
     1261 tccgatttca ggcccatctg gccgatgccg tcgaccttgc agtcgatgtc gtggtcgcca
     1321 tccaccaggc ggatgttgcg caccttggtg cccaccttga ccaccagcga cgagcccttg
     1381 agcttgaggt ccttgatcac ggtgacggtg tcgccgtcct gcaacacgtt gccgaccgcg
     1441 tcgcggacca ccttcgcgtc atcgacggcg tcggcctgca gcggcgacca ctcatgtccg
     1501 cactccgggc agaccagcat cgcgccgtcc tcgtaggcgt aggcggaggc gcattgcggg
     1561 cagggcggca gggtgctcat cgtggcagtc ttcgcggaga cggaggtggc gccattttac
     1621 gccgcgcccg cggcggttca gggcaccgcg ggccacgatg tcgatgcaag caggcaggcc
     1681 gcatcgtcga caatggcccc ggacccctgc ggccgttccc tggagcgaac catgcacgtg
     1741 atcgacctgc gcagcgacac ggtgacccaa cccacccgcg cgatgcgcgc ggcgatgctc
     1801 gaggcgcggg tcggcgacga cgtctacggc gaggacccca ccgtcaacgc actgcaggag
     1861 cggctggccg cggagctggg gttcgacgcc gggctgttcg tgcccaccgg cacccagtcc
     1921 aacctgctgg ccctgctggc gcattgcgaa cgcggcgacg aatacctggt cggcatggaa
     1981 gcgcatacct acaagtacga gggcggcggc gcggcggtgc tggggtcgat ccagccgcaa
     2041 ccgatcgtcc aggccacgga tggctcgctg ccgctggaca gcatcgccgc cgccatcaag
     2101 ccggtggatc cgcacttcgc ccgcacccgc ctgctggcgc tcgagaacac ctggcatggc
     2161 cgcgcgctgc cgctggacta cctgcgcagc gcgcatgcgc tggcgcgcgc gcatggcctg
     2221 gggatgcacc tggacggcgc acgcctgttc aacgccgcgc tggcatgcca ggtgccgccg
     2281 cgcgagatcg cgcgccactt cgacacggtg tcggtctgcc tgtccaaggg gctgggcgcg
     2341 ccggtcggtt cggtgctggt gggctcacgg gcgctgatcg acaaggcgcg ccgctggcgc
     2401 aaggtctgcg gcggtggctg gcggcaggcc ggcctgctgg ccgcggcggc actgcatgcg
     2461 ctggaccacc acgtggcacg cctggccgag gaccatgcac gcgcggccat gctcgcggag
     2521 cgcctgcgcg ggatcggcgg catcgacctg cttggccagc acaccaacat ggtcttcatc
     2581 gacgtgccgg cggagcgcct gcaggcactg gatgcgcacc tgcgcacggc aggcatccgc
     2641 gccagcatcg gctacctgcc gaccctgcgg ctggtgaccc acctcgacat cgacgacgac
     2701 ggcatcgaac gcaccgcggc ggcgttcgca cggttcttcg nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn ccggtcagtc cagcgtgccg cgcggcagca ccaactcgtc
     2881 gaccggataa ccgcgccggg cggcgcgtgc gcggatcccg tcgtactgcg cgcggtccat
     2941 gctgggcgtg cgcgagagga tccacaggta cttgcggctg ggtccgccga ccaccgccca
     3001 gcggtagtcg gggtcgaggt cgaccaccca gtagtccgcc cacgccatcg gtgcccaggc
     3061 cagccagtcg ggcgcgaagc gcacctgcag ccgccccggg tgcccggcca ccggtcgcgc
     3121 cgtgccctcg gacagcatcc ggccgccgtc cttgtcgtcg caggcgttgc gcacgcccac
     3181 caggccatcg gcgcgcagcg tgtaggtggc ggtgatgttc gcaacgcagt tgcgctggaa
     3241 gaacatcggc aggcgtgcga tctcgtgcca ctcgccgctg tagcgctgca ggtccatcgc
     3301 cggcaccgac gcgttgggca gcgccgcgtc ccgggcatcg gcggtaccgc cggcaagggc
     3361 tattgcgagc agcagggatc gaatcatgtt cggcgcttca tgggtccggg cacgcagtgt
     3421 ctccaacttt gcgttcgccc atggtgaatg cgctccgcgg gtgcatccgg aagccggccc
     3481 gccgcgtatc gccatgacca cgccgcggat cgatccgtga acgcgtgagc ccccaacacg
     3541 aaccggagcg tccccatgaa gtccaccctg cccctcgcca ttgccagcgc cctcgccctc
     3601 gtcgcctgcg gcgcatccga acccgcggcc gccgatcctg cgccggcccc gatggaaacc
     3661 gccgccgaac cgacgccggc cgagccggtc gcggccaccg ccagcaccgc cccggatgcc
     3721 gccaatgaca tcgtcaccac tgcctccgcc acgcccagcc tgagcaccct ggtggctgcg
     3781 gtgaaggccg ccgagctggt cgccaccctg cagggcgccg gcccgttcac cgtgttcgca
     3841 cccaccaacg atgccttcgg caagctgccg gcaggcgcgg tggatggcct gctgaagccg
     3901 gaaatgaagg ccgacctggc cggcgtgctg acctaccacg tggtgtccgg caacgtcgac
     3961 gcggccaccc tgttgggcca gatcgaagcc ggcggcggca ccgccaccct gaccaccgtc
     4021 cagggcggca ccctgaccgc caccccggac ggcgccggcg gcatcaccct gaccgacgcc
     4081 aagggcggta ccgcgaaggt caccactgcc gacctccgcc agagcaacgg catcgtgcat
     4141 gtcatcgaca gcgtgctgat gccgaagtaa tc
//
LOCUS       DAORUK010000173         4090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_28163_length_4090_cov_18.426766, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000173 DAORUK010000000
VERSION     DAORUK010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28163_length_4090_cov_18.426766"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PK818_09590"
     CDS             <1..802
                     /locus_tag="PK818_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017165008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HQY83021.1"
                     /translation="EFLHRLPFYGLAVLCIDDPEVALLAGNTPRHVVTYGFAAEADVR
                     AEGVSQQGARMQFTLCLPDASRTAVTLALPGRHNVQNALAAAAVAWQLGVGNASIARA
                     LEQFAGIGRRFNQLATLPLAGGGEVVLVDDYGHHPKELAAVFDAARGGWADKRLVVAF
                     QPHRYSRTRDLFDEFAAVLSGVDALVLTEVYPAGEAPIAGADAKALARSIRTRGRVDP
                     VVVGSAAELGDALAGVLRDGDLLLMMGAGDIGAAAQSIARNGFGGGDA"
     gene            799..1767
                     /locus_tag="PK818_09595"
     CDS             799..1767
                     /locus_tag="PK818_09595"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HQY83022.1"
                     /translation="MNATTFPPLRITDPRAFGRVAVLMGGTSAEREVSLDSGRNVLQA
                     LQARGVDAHAVDGIPALVDALVHGKFDRVFNILHGNKGGGEDGVLQGLLEAFEVPYTG
                     CGVLASALTMDKIRTKQVWMSAGLPTPTFVRLAPGDGVRAAALELGLPVFVKPSSEGS
                     SVGVARVVDAAAIDEAIRVAREYGGEMLMEQMIVGDELTVGILGDVALPSIRIVPKGE
                     WYDYNAKYVADDTQYLCPGLDGAQEDEIRRIALAAFRAAGCTGWGRVDVMRERASGQL
                     TLLEVNTAPGMTSHSLVPKAARQLGVEFEELCWRILEQSMPEAATP"
     gene            1773..2549
                     /locus_tag="PK818_09600"
     CDS             1773..2549
                     /locus_tag="PK818_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HQY83023.1"
                     /translation="MLRLLAWILALALVALPVVAVVNGWVGGAQFPLRTLRVQGELQR
                     VDAARLRATVLPHARRGFFAVQPERIQAAVAALPWVERVEVRKHWPDVLELRISEHRP
                     FARWGGGRLLSEQGRLFPARGIAVPRGLPLLDGPDARVADVVALYNQARERLANAGGV
                     RGVALDRRGSWSMTLHGGTEVVLGRNDPDARLQRFAPLLPRLAAQHPGQRLARADLRY
                     TNGFSLTWVDLPKAPENALLLPQPVAALSRSGSSSMEGTS"
     gene            2546..3781
                     /gene="ftsA"
                     /locus_tag="PK818_09605"
     CDS             2546..3781
                     /gene="ftsA"
                     /locus_tag="PK818_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HQY83024.1"
                     /translation="MNRKGDKSLIVGLDIGTSKVTALVGEYAPGEPIEVIGIGSHESR
                     GLRRGVVVDIDSTVQSIQRAVEEAELMAGCEVRSVYASISGNHVQCRNSQGIAPIREG
                     EVTYADLDRVLEAAKAVAIPADQKILHAIPRDYVLDDSQEGIRNPVGMTGVRLEVHAH
                     LVVCAQSAAANISKCVQKCGLQVDSLILSALASSQAVLTADERELGVAMVDIGAGTTD
                     IAVYVQGAICHTASLPVAGDKVTEDIAHMLRTPTPEAEQIKVRYACALAQLARAEESI
                     QVPSVGDRPPRRLPRHALAQAVQARYEEIFEMVQAELRRSGFEQLVRAGMVLTGGASK
                     MEGVVELAEEMLQMPVRIGIPQGVTGLGEVTGNPVHATGVGLLLMGSQIENPRRSVLP
                     GNKVGGLLGKLSKWYRGEF"
     gene            3913..>4090
                     /locus_tag="PK818_09610"
     CDS             3913..>4090
                     /locus_tag="PK818_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017170527.1"
                     /note="GTPase; similar structure to tubulin; forms
                     ring-shaped polymers at the site of cell division; other
                     proteins such as FtsA, ZipA, and ZapA, interact with and
                     regulate FtsZ function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HQY83025.1"
                     /translation="MAHFELVEKMAPNAVIKVVGVGGGGGNAVAHMVSSSVDGVEFIT
                     ANTDSQAIKNCGAKT"
BASE COUNT          609 a         1370 c         1536 g          575 t
ORIGIN      
        1 cgaattcctg caccggctgc cgttctacgg gctggcggtg ctgtgcatcg acgatcccga
       61 ggtcgcgctg ctggcgggca acaccccgcg ccacgtggtg acctacggtt tcgccgccga
      121 ggccgacgtg cgcgccgagg gcgtctccca gcagggcgcg cgcatgcagt tcaccctgtg
      181 cctgccggac gcatcgcgca ccgcggtgac gctggcgctg ccgggacgcc acaacgtgca
      241 gaacgcgctg gccgccgccg ccgttgcctg gcaactgggg gtcggcaacg cgtccatcgc
      301 ccgcgcgctc gagcaattcg ccggcatcgg ccgccgcttc aaccagctgg cgacgttgcc
      361 gctggcgggc ggtggcgagg tggtgctggt ggacgactac ggccaccacc cgaaggaact
      421 tgcggcggtg ttcgatgcgg cgcgcggtgg ctgggccgac aagcggctgg tggtggcatt
      481 ccagccgcac cgctattcgc gtacccgcga cctgttcgac gaattcgccg cggtcctgtc
      541 cggtgtcgat gcgctggtcc tgaccgaggt gtatccggcc ggcgaagcac cgatcgccgg
      601 cgccgacgcg aaggcgctgg cgcgctccat ccgcacccgc ggccgggtcg atccggtggt
      661 ggtcggcagt gccgccgagc ttggcgacgc actcgccggg gtgctgcgcg acggcgacct
      721 gctgctgatg atgggggccg gcgacatcgg cgcggccgcg cagtcgatcg cgcgcaacgg
      781 cttcggcggg ggcgatgcat gaacgccacc acgttcccgc cgctgcgcat caccgacccg
      841 cgcgcgtttg gccgggtcgc ggtgctgatg ggcggcacca gcgccgagcg cgaggtctcg
      901 ctggattccg gccgcaacgt gctgcaagcg ctgcaggcgc gcggcgtgga cgcgcatgcg
      961 gtggacggga tcccggcgct ggtcgatgcg ctggtccacg gcaagttcga ccgcgtgttc
     1021 aacatcctgc acggcaacaa gggcggcggc gaagacggcg tgctgcaggg cctgctggaa
     1081 gccttcgaag tgccgtacac cggctgcggg gtgctggcca gcgcgctgac catggacaag
     1141 atccgcacca agcaggtgtg gatgagcgcg ggcctgccga cgccgacgtt cgtgcggctg
     1201 gcgccgggcg atggcgtgcg cgcggcggcg ctggagcttg gcctgccggt gttcgtgaag
     1261 ccgtccagcg aaggttccag cgtcggcgtc gcccgcgtgg tcgacgcggc cgcgatcgac
     1321 gaagccatcc gcgtcgcgcg cgagtacggc ggcgagatgc tgatggagca gatgatcgtc
     1381 ggcgacgagc tgaccgtcgg catcctcggc gatgtcgcgc tgccgtcgat ccggatcgtg
     1441 ccgaagggcg agtggtacga ctacaacgcc aagtacgtcg ccgacgacac ccagtacctg
     1501 tgccccggcc tggacggcgc gcaggaagac gagatccgcc gcatcgcgct ggccgcgttc
     1561 cgcgccgccg gctgcaccgg ctggggccgg gtcgacgtga tgcgcgagcg cgccagcggc
     1621 cagctcaccc tgctggaggt gaacaccgcg ccgggcatga ccagccactc gctggtgccg
     1681 aaggccgcgc gccagctcgg cgtcgagttc gaagaactgt gctggcggat cctcgaacag
     1741 agcatgccgg aggccgcgac cccgtgagcg cgatgctgcg actgctggcg tggatcctgg
     1801 ccctggcact ggtggcgttg cccgtcgtgg ccgtggtcaa cggctgggtc ggcggcgcgc
     1861 agttcccgct gcgcacgctg cgcgtgcagg gcgaactgca gcgggtggac gcggcgcggt
     1921 tgcgcgcgac cgtgctgccg catgcgcggc gcggattctt cgcggtgcag ccggagcgca
     1981 tccaggccgc ggtcgccgca ttgccgtggg tggagcgggt ggaagtgcgc aagcactggc
     2041 cggacgtgct ggagctgcgg atcagcgagc accggccgtt cgcgcgctgg ggcggcggtc
     2101 gcctgctgtc cgagcagggc cggctgttcc cggcccgtgg catcgcggta ccgcgcggac
     2161 tgccgttgct ggacggaccc gatgcgcgcg tcgccgacgt ggtggcgctg tacaaccagg
     2221 cgcgcgaacg cctcgccaat gccggcggtg tgcgcggggt ggcgctggac cgccgcggca
     2281 gctggtcgat gaccctccac ggcgggaccg aagtcgtgct cggccgcaac gacccggatg
     2341 cgcgcctgca gcgcttcgcc ccgctgctgc cgcggctggc cgcgcagcat cccgggcagc
     2401 ggctggcgcg cgcggacctg cgctacacca acggtttctc gctgacctgg gtcgacctgc
     2461 cgaaggcccc ggaaaacgca ttgctgctcc cccagcccgt cgcagcgttg tcgcgcagcg
     2521 ggtcttcgtc gatggaagga acctcatgaa tcgcaagggc gacaagtcgc tgatcgtcgg
     2581 cctcgacatc ggcacctcca aggtcaccgc gctggtcggc gagtacgcgc cgggcgagcc
     2641 gatcgaggtg atcgggatcg gttcgcacga atcgcgcggc ctgcgccgcg gggtggtggt
     2701 ggacatcgac tccaccgtgc agtcgatcca gcgcgcggtg gaggaggccg agctgatggc
     2761 cggctgcgaa gtgcgcagcg tgtacgcctc gatctccggc aaccacgtgc agtgccgcaa
     2821 ttcgcagggc atcgccccga tccgcgaggg cgaggtcacc tacgccgacc tcgaccgcgt
     2881 gctggaagcg gccaaggcgg tggcgatccc ggccgaccag aagatcctgc atgcgatccc
     2941 gcgcgactac gtgctcgacg attcgcagga aggcatccgc aacccggtcg gcatgaccgg
     3001 cgtgcgcctg gaggtgcacg cgcacctggt ggtgtgcgcg cagtcggccg cggccaacat
     3061 cagcaagtgc gtgcagaagt gcggcctgca ggtggacagc ctgatcctgt ccgcgctggc
     3121 ctccagccag gcggtgctga ccgccgacga acgcgagctg ggcgtggcga tggtcgacat
     3181 cggcgccggc accaccgaca tcgcggtcta cgtgcagggc gcgatctgcc acaccgccag
     3241 cctgccggtc gccggcgaca aggtcaccga ggacatcgcg cacatgctgc gcacgcctac
     3301 ccccgaggcc gagcagatca aggtccgcta cgcctgcgcg ctggcgcagc tggcgcgcgc
     3361 cgaggaatcg atccaggtcc cgagcgtcgg cgaccgcccg ccgcggcggc tgccgcggca
     3421 cgcgctggcg caggcggtgc aggcgcgtta cgaggagatc ttcgagatgg tgcaggccga
     3481 attgcgccgc tccggcttcg agcagctggt gcgcgccggg atggtgctga ccggcggcgc
     3541 ctcgaagatg gaaggcgtgg tcgagctggc cgaggagatg ctgcagatgc cggtgcggat
     3601 cggcatcccg cagggggtga cgggcctcgg cgaggtgacc ggcaacccgg tgcacgccac
     3661 cggcgtgggc ctgctgctga tgggcagcca gatcgagaac ccgcggcgct cggtgctgcc
     3721 gggcaacaag gtcggcggcc tgctcggcaa gttgtccaag tggtaccgcg gcgaattctg
     3781 agattcaacg ccggcgcgag ggcgcggcgg cgtgcggtgg gtgggaggca ggggccggcg
     3841 acacgacatc caaccaagca acagattccg acaagcagac aaccaagcaa ttcataacaa
     3901 gaggacacgg acatggcgca cttcgaactg gttgaaaaga tggcaccgaa cgcggtgatc
     3961 aaggtcgtgg gcgtgggcgg tggcggcggc aacgccgtgg cgcacatggt cagcagcagc
     4021 gtggacgggg tggagttcat caccgccaac accgactcgc aggcgatcaa gaactgcggc
     4081 gcgaagaccc
//
LOCUS       DAORUK010000174         4069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_28410_length_4069_cov_17.242152, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000174 DAORUK010000000
VERSION     DAORUK010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28410_length_4069_cov_17.242152"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1637)
                     /gene="ctaD"
                     /locus_tag="PK818_09615"
     CDS             complement(<1..1637)
                     /gene="ctaD"
                     /locus_tag="PK818_09615"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HQY83026.1"
                     /translation="MSAPLEPAIPPRRALRLHRELTAIWATGKGLQRLASVNHSVIGL
                     RIMITAFVFMLIGGLLGMLTRVQLATPDGKFMAPETYNQVFTMHGVMMLFLFAIPMLE
                     GVAVYLLPKMLGARDFAFPRLTAFSYWCYLFGASILTGALLLGVAPDGGWFMYTPLSS
                     NTYTPGVNADVWLLGVTFVEISALSFAVEIVVSILKMRAPGMSIDRMPIFGWYMLVTA
                     LMMLVAFPPLILASILLEVERAFGLPFFDPTRGGDALLWQHLFWLFGHPDVYIIFLPM
                     AGALSTMIPVFAGRALVGYRIIVVAIVGMGFLSFGIWVHHMFTVGIPHLALAFFAAGS
                     AIVAVPTAVQMFAWLATLGTKRPRWDLPMLYIFGFFFVFVLGGLTGVMLAMVPFDWQA
                     HDTYFVVAHLHYVAAGSFLFPLIAALYYWLPLLTGRVADKRLAVPAFWLTFIGFNLTF
                     FIMHLVGLRGMPRRIYGYEAQDGWTWMNLLSSVGSFIMVIGIGLLLIDLVVQLRYGRR
                     ARRDPWRATTLEWAMPIPPRPYNFASIHHLDTHGDQVA"
     gene            complement(1634..2287)
                     /gene="coxB"
                     /locus_tag="PK818_09620"
     CDS             complement(1634..2287)
                     /gene="coxB"
                     /locus_tag="PK818_09620"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HQY83027.1"
                     /translation="MLAGCSGPTSTLDPAGPAAAAIAGMWWVMLAGSIVLFVLVMALF
                     GLAWLRPGWSLAMPLHRWALLGGLALPALVLVPLVAYALVTGERLMPLPGMSPPRIEV
                     HAERWRWTFRYPDHGGAVSEGVLHIPAGAPVDLVITSGDLIHSLWIPRLGGKMDAIPG
                     RVNVLRLQADAPGRYAGRCAEFCGTGHTVMRFEVIAHPAADYAAALARAGATTEANP"
     gene            2366..2881
                     /locus_tag="PK818_09625"
     CDS             2366..2881
                     /locus_tag="PK818_09625"
                     /inference="COORDINATES: protein motif:HMM:NF021505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HQY83028.1"
                     /translation="MAKIEQEPIDAARSPVLLEVQKLDTNSIVALVGHPLHVMMVHFP
                     VAFVIATLGADVFYWWSGDAFWVRAGLWAAGVAFWTGVAASAVGTAELLLVRGIRLLE
                     ASWSHAVAAMTLLAVAGANWGLRLAYPESILPHGLALSALASVMTGFAGWHGGKLVFD
                     HGVGVLVSPKS"
     gene            complement(2845..3357)
                     /locus_tag="PK818_09630"
     CDS             complement(2845..3357)
                     /locus_tag="PK818_09630"
                     /inference="COORDINATES: protein motif:HMM:NF015608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HQY83029.1"
                     /translation="MTTFLKFVHLATIALWSGGLIVLPMLFWQRRHVTNVAELERLHR
                     VTRYVYVVLTSPAAVVAIGTGTALIFLQTTFREWFSLKMVLVGAMAMLHVLAGLTAVK
                     VFGPNGRMGTRACIVLTSLYLLVIVAILWVVLAKPGIDANEIAPHLFDPGGLPALLDQ
                     LFGETRTPTP"
     gene            complement(3354..>4069)
                     /locus_tag="PK818_09635"
     CDS             complement(3354..>4069)
                     /locus_tag="PK818_09635"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HQY83030.1"
                     /translation="IAYCGPAAIPDGFWTRWNTDPLLIAALFGLALLVHRSHAGNRRA
                     GWAAIGVLFLTFVSPLCALSSALFSARVLHHVLLVAAAAPLLALAFPSRRLGALPLGL
                     LVGAHAVVMWAWHTPPAYSWGLASVPAYWLMQATLLGSAWLLWRGIFAPAAANGMAYM
                     ALGATIFQMGLLGALIVFAPYPLYAEHFTSTDPWGLHPLADQQLAGLLMWVPAMLPYV
                     AVAIRLAWTGLRGAGAQAT"
BASE COUNT          758 a         1342 c         1454 g          515 t
ORIGIN      
        1 gcgacctggt cgccgtgggt gtccagatgg tggatcgagg cgaagttgta gggccggggc
       61 gggatcggca tggcccactc cagcgtggtc gcgcgccacg ggtcgcgccg cgcgcgccgc
      121 ccgtagcgca actgcaccac caggtcgatg agcagcaggc cgatgccgat gaccatgatg
      181 aaggagccga ccgaggacag caggttcatc caggtccagc catcctgcgc ctcgtagccg
      241 tagatgcgcc gcggcatacc gcgcaggccg accaggtgca tgatgaagaa ggtgaggttg
      301 aagccgatga aggtcagcca gaaggccggc accgcaaggc gcttgtcggc gacgcgcccg
      361 gtcaacagcg gcagccagta gtagagcgcc gcgatcaggg ggaacaggaa cgagccggcc
      421 gcgacgtagt gcaggtgtgc gaccacgaag taggtgtcgt gggcctgcca gtcgaagggc
      481 accatggcca gcatcacgcc ggtcagtccg ccgagcacga acacgaagaa gaagccgaag
      541 atgtacagca tcggcaggtc ccagcgcgga cgcttggtgc ccagcgtggc cagccacgcg
      601 aacatctgca ccgcggtcgg caccgcgacg atcgccgagc cggcagcgaa gaacgcgagc
      661 gcgaggtgcg ggatgcccac cgtgaacatg tggtgcaccc agatgccgaa gctcaggaat
      721 cccatgccga cgatggccac cacgatgatg cggtagccga ccagcgcgcg cccggcgaac
      781 accgggatca tggtcgacag cgcgccggcc atcggcagga agatgatgta cacgtccggg
      841 tgcccgaaca gccagaacag gtgctgccac agcagcgcgt cgccgccgcg cgtggggtcg
      901 aagaagggca ggccgaaggc gcgctccacc tcgagcagga tcgaggccag gatcagcggc
      961 gggaacgcca ccagcatcat cagcgccgtg accagcatgt accagccgaa gatgggcatg
     1021 cggtcgatcg acatgcccgg cgcgcgcatc ttcaggatcg agaccacgat ctcgaccgcg
     1081 aaactcaggg ccgagatctc gacgaaggtc acgcccagca gccagacatc ggcgttgacg
     1141 ccgggcgtgt aggtgttcga actcagcggc gtgtacatga accagccgcc gtccggcgcg
     1201 acgcccagca gcagcgcgcc ggtcaggatc gacgcgccga agagataaca ccagtagctg
     1261 aaggcggtca ggcgcgggaa ggcgaagtcg cgcgcgccga gcatcttcgg cagcaggtac
     1321 accgccacgc cttcgagcat cgggatcgcg aacaggaaca gcatcatcac gccgtgcatc
     1381 gtgaagacct ggttgtaggt ctccggcgcc atgaacttgc cgtcgggcgt ggcgagctga
     1441 acccgcgtga gcatgccgag caggccgccg atcagcatga acacgaacgc ggtgatcatg
     1501 atccgcaagc cgatcaccga gtggttcacg gacgccagtc gctgcagtcc cttgccggtg
     1561 gcccagatcg cggtgagctc gcggtgcagc cgcaaggcgc gccgcggcgg gatcgcgggt
     1621 tcgagtggcg cgctcatgga ttggcttccg tggtggcgcc tgcccgggcc agcgcggcgg
     1681 cgtagtccgc cgcgggatgg gcgatgacct cgaatcgcat cacggtatgg ccggtgccgc
     1741 agaactcggc gcagcgcccg gcatagcggc cgggcgcatc ggcctgcagc cgcagcacgt
     1801 tcacgcgccc gggaatcgcg tccatcttgc cgcccaggcg cgggatccac agcgagtgga
     1861 tgagatcgcc actggtgatc accaggtcca ccggcgcgcc ggcgggaata tgcagcacgc
     1921 cttcactcac ggcgccgcca tggtccgggt accggaaggt ccagcgccag cgttcggcgt
     1981 gcacttcgat gcgcggcggg ctcatgcccg gcagcggcat caggcgctcg ccggtgacca
     2041 gtgcgtaggc caccaggggc accaggacca gggcgggcag ggccaggccg ccgagcaagg
     2101 cccagcgatg cagcggcatg gccaggctcc agcccggccg cagccaggcc aacccgaaca
     2161 aggccatgac cagcacgaac agcacgatcg agccggccag catcacccac cacatgccgg
     2221 cgatcgctgc agcggcgggg cccgctggat ccagggtcga ggtcggcccg ctgcagccgg
     2281 ccagcatggc gatcgtcgtg gcgaccagcg cggcgcgcgc aaggctatga tgcgagcgtc
     2341 gagtccggca cccgggagcc gcgccatggc gaagatcgag caggaaccca tcgatgcggc
     2401 ccggagtccg gtgctgctcg aggtgcagaa gctcgatacc aactcgatcg tggcgctggt
     2461 gggccatccg ctccacgtga tgatggtgca cttcccggtc gcgttcgtga tcgcgacgct
     2521 tggcgccgac gtcttctact ggtggtcggg cgacgcgttc tgggtacgcg ccggcctgtg
     2581 ggccgcgggc gtcgccttct ggaccggcgt cgcggcgagc gcggtgggca cggccgagtt
     2641 gttgctggtg cgcggcatcc gcctgctcga ggccagctgg tcgcatgccg tggccgccat
     2701 gaccctgctc gcggtcgccg gcgccaactg gggcctgcgg ctggcctacc ccgagtcgat
     2761 cctgccgcac ggcctggcac tgtcggcgct ggcgtcggtg atgaccggtt tcgccggctg
     2821 gcatggcggc aagctggtct tcgatcatgg cgtcggcgtc ctggtctcgc cgaagagctg
     2881 atcgagcagc gcgggcagtc cgcccgggtc gaagaggtgc ggggcgatct cgttggcatc
     2941 gatgccgggc ttggccagca cgacccacag gatcgcgacg atcaccagca ggtagagact
     3001 ggtcagcacg atgcaggcgc gcgtgcccat gcggccgttc gggccgaaca ccttcaccgc
     3061 ggtcaggccg gccagcacgt gcagcatggc catcgcgccg accagcacca tcttgagcga
     3121 aaaccactcg cggaaggtgg tctgcaggaa gatcagcgcg gtgcccgtgc cgatcgcgac
     3181 tactgcagcc ggcgacgtca gcacgacata gacgtagcgc gtgacccgat gcaggcgctc
     3241 gagttcggcc acgttggtga catggcggcg ctgccagaac agcatcggca gcacgatcaa
     3301 gccgcccgac cacagcgcga tggtggcgag atggacgaac ttgaggaagg tcgtcatgtg
     3361 gcctgcgcgc ctgcgccgcg caggcccgtc catgccagcc ggatcgcgac ggcgacgtaa
     3421 ggcaacatcg ccggtaccca catcaggagg cccgccagtt gctggtcggc cagcgggtgc
     3481 aggccccagg gatcggtgct ggtgaagtgc tcggcataca ggggataggg cgcgaacacg
     3541 atcagggcgc cgagcagtcc catctggaag atggtggcgc ccagtgccat gtacgccatg
     3601 ccattggccg ccgccggcgc gaagatcccg cgccacagca gccaggcgct gccaagcagc
     3661 gtcgcctgca tcagccagta ggcgggcacg ctggccaggc cccaggaata ggccggcggc
     3721 gtatgccagg cccacatgac aacggcatgc gcgccaacca gcagccccag cggcagtgcg
     3781 ccgaggcgac gcgacggaaa ggccagcgcg agtagcggag cggccgccgc gaccagcagc
     3841 acgtggtgca gcacgcgcgc agagaacagc gccgaggaca gcgcgcacaa cggtgagacg
     3901 aaggtcagga acaacacgcc gatggcggcc cagccggcgc gccggttgcc ggcgtggctg
     3961 cgatggacca gcaaggccag cccgaacagc gccgcgatga gcagcggatc ggtgttccag
     4021 cgggtccaga atccgtccgg aatcgcggca gggccgcaat aggcgatca
//
LOCUS       DAORUK010000175         3986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_29311_length_3986_cov_21.132027, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000175 DAORUK010000000
VERSION     DAORUK010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29311_length_3986_cov_21.132027"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1044)
                     /gene="rquA"
                     /locus_tag="PK818_09640"
     CDS             complement(91..1044)
                     /gene="rquA"
                     /locus_tag="PK818_09640"
                     /inference="COORDINATES: protein motif:HMM:NF038261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodoquinone biosynthesis methyltransferase
                     RquA"
                     /protein_id="HQY83031.1"
                     /translation="MPNHTPAGAYHHVARTAESRIQRAIAPPRPAPNDPRQSTRARIR
                     ASSGDTILTRSGCMASEAEVAALAPDALPASDTPAAERWQRFHADGIPEYLARNYWWA
                     YLSRPGMAFFDWQPVVNLILIGQYRRLMDLALARIDRDSARTLQIACVYGQLTPRLAG
                     HLRSGTLDVVDVAPAQLGLCARKLAAVGGDARLTRMDAESLALADGSYDHVLMFCLLH
                     EIPPATRAAALGEALRVLKRGGRLVIVDFGEAGCGKRSRVLDAWRRVLRRMEPFLGGF
                     IAEDLVAGLRAAAQRVGRRVEAIKQVAVLGGLYRVVEVRID"
     gene            1078..2259
                     /locus_tag="PK818_09645"
     CDS             1078..2259
                     /locus_tag="PK818_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83032.1"
                     /translation="MKSLASVLACVVLASVASAGTPPTGAADPNQIPNALLLDRNGAV
                     AGNVTGTAPTLWRAIALTGGAMDVSSVALAADALFPGSPPATAVRISVSAFGTDQVFD
                     HAPALFTIVPGRTYQARIYVRSGNADASPQTFRFGMPLFDTALAFTGRDPANFTQPAT
                     SAWTAFDSPTFTADPGDAYGHVSFRLQNDGGENTILVALPTLLGPAVANEAPNPAFTG
                     TGGASQGTVTGAIPNAWRAFAVGAGSLTVATEPLAAGALFPGSTATTAVRLQVTGGNG
                     SSEGFDHQQVRALLAPGHRYWSEVWIRAGGATDQGVTISMPIFDAGGTFTGNQPGSTV
                     AIVGPQWRFVAGPPFSGAAGETANLAFRLAADGGEDSLLIAAPRIVGPARPVIFGNGF
                     E"
     gene            2352..3212
                     /locus_tag="PK818_09650"
     CDS             2352..3212
                     /locus_tag="PK818_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83033.1"
                     /translation="MQREGQRRTGVPTPVPVASTRRAHAAAPATDRKPPMRTPRIIAA
                     AVALALLGAPPAHAAFHLMKVVEVFPGAPAAPNAQYVVLQMYSGGQTLVSGHVVTVYN
                     AAGTPVGTYTFGANVANGAAQDKILIATTEAQTFFGITANLIMTPTVAAAGGKVCFDS
                     SPIDCVAWGNYSSAPTGVGTPFNDPVLGGLATGRAAARRLDITGFPGVLDAGDDTGDS
                     ANDFRTALPRPTNNARQIGTIPPSTCGNLAIEGVEQCDDGDLDNGDGCSSTCAIEPPL
                     PPLIYANGFE"
     gene            complement(3216..>3986)
                     /locus_tag="PK818_09655"
     CDS             complement(3216..>3986)
                     /locus_tag="PK818_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83034.1"
                     /translation="PHRGEALWILSGIHGEEPAGPNAIAENITIIAELAATGVPMVVI
                     PLANPQAYRHNWRYPNTAERDWKKGGYSVGDAEYLLPDLEQGGGPRAAAAPGPETAAL
                     TQFVLRMAQHYPPRLVLDLHEDELSTAGGYIYSQGTQPDDNPVGAEVVRLLQATGIPL
                     RQSGKTRFGEPVVKGVISRDDAGAPIRDGSIDELLAATEVFVDGRKRAGPAARTVIVV
                     ETPAFEGSRFELRVAAQGAVVQRLPVLWQLSSDVREKP"
BASE COUNT          600 a         1474 c         1318 g          594 t
ORIGIN      
        1 cgcgacatgg gcctatcgcg cgcagagcgc gctcctacag agccggcgtt gtaggggcgc
       61 gctctgcgcg cgatcagcgc ctcacttccc tcagtcgatc cgaacctcga ccacccgata
      121 caaccccccc agcaccgcaa cctgcttgat cgcctccacc cgccgcccca cccgctgcgc
      181 cgccgcgcgc aatccggcta ccagatcctc ggcgatgaac ccgccgagga acggctccat
      241 ccgccgcagc acgcgccgcc atgcgtctag cacccgcgag cgcttgccgc acccggcttc
      301 gccgaaatcc acgatcacca ggcgcccacc gcgcttgagc acgcgcagcg cctcgcccag
      361 cgccgccgcc cgcgtcgccg gcggaatctc gtgcagcagg cagaacatca gcacatggtc
      421 gtagctgcca tcggccagcg cgagcgactc cgcgtccatc cgcgtcagcc gcgcatcacc
      481 gcccacggcc gccagcttgc gcgcgcaaag gccaagctgc gccggcgcga catcgaccac
      541 gtccagcgtg ccgctgcgca ggtgccccgc aagccgcggc gtgagctgcc cgtacacgca
      601 ggcgatctga agggtgcgcg cgctgtcgcg gtcgatgcgc gccagcgcca agtccatgag
      661 ccgccggtac tgcccgatca ggatcaggtt gaccaccggc tgccagtcga agaaggccat
      721 gcccggccgc gagaggtagg cccaccagta gttgcgggcg aggtactcgg ggatgccgtc
      781 ggcgtgaaag cgctgccagc gttccgccgc gggcgtatcc gacgccggca gggcgtccgg
      841 cgcgagggca gcgacttcgg cttcggaggc catgcatccg ctccgcgtca ggatggtgtc
      901 gccagatgat gcgcggattc gggcacgcgt ggattgacgt gggtcatttg gcgctggacg
      961 cggcggggca attgcccgct gaatgcggga ttcggcggta cgggcgacgt gatgatacgc
     1021 gccggcgggc gtatgattcg gcatgtcccc gcttcgctcg tcgcaagagg atcgaccatg
     1081 aagtcgctgg cgtccgttct tgcatgcgtc gtgctcgcct cggttgcgtc cgccggcacg
     1141 ccgccgaccg gcgcggcgga cccgaaccag attcccaatg cgctgctcct ggatcgcaac
     1201 ggcgcagttg ccggcaacgt aaccggcacc gcacccacgc tctggcgcgc gatcgcgctg
     1261 accggcggcg cgatggacgt ctcgagcgtt gcgctggcgg ccgacgcgct gttccccggc
     1321 agcccgcccg ccacggcggt caggatcagc gtcagcgcgt tcggcacgga ccaggtgttc
     1381 gaccatgcgc cggcgctgtt caccatcgtg cccggccgga cgtaccaggc acgcatctac
     1441 gtgcgcagcg gcaatgccga cgcgtcgccg cagacgttcc ggttcggcat gccattgttc
     1501 gacaccgcgc tggcgttcac cgggcgcgac ccggcgaact tcacccaacc cgcgacgtcc
     1561 gcatggaccg cattcgacag ccccacgttc accgccgacc ctggcgacgc ctacggccac
     1621 gtctcgttcc gcctgcagaa cgacggcggt gagaacacga tcctggtcgc attgccgacg
     1681 ctgctcgggc ctgcggtcgc gaatgaagcg ccgaatcccg ccttcaccgg caccggcggt
     1741 gcgtcgcagg gcacagtgac cggtgcgatt cccaacgcct ggcgcgcctt cgcggtgggc
     1801 gccggatcgc tgacggtcgc gaccgaaccc ctcgccgcgg gcgcgctgtt ccccggcagc
     1861 acggcgacca ccgcggtgcg cctccaggtc accggtggca atggcagcag cgagggtttc
     1921 gaccaccagc aggttagagc cctgctggcc cccggacatc gctactggag cgaagtctgg
     1981 atccgcgccg gcggcgcgac cgaccagggc gtgacgatct cgatgccgat tttcgacgcg
     2041 ggcggtacct tcaccggcaa ccagcccggc tccaccgtcg cgatcgtcgg gccgcagtgg
     2101 cgattcgtcg ccggcccgcc gttttccggc gcggccggcg agaccgccaa ccttgcattt
     2161 cgcctcgcgg ccgacggtgg cgaggacagt ctgcttattg cggcgccgcg catcgtcggg
     2221 cctgcgcggc cggtgatctt cggcaacggc ttcgaatgac gcaactgtgt cggtgtcccg
     2281 gcctacctcc ggatgatgcg cgaatcgggg tttggccgga ttgacgtggg tcaggtggtg
     2341 caggccgcga cgtgcagcgc gaaggacagc gtcgaactgg agtccccacc cccgtcccgg
     2401 tagcctccac ccgccgggcg cacgccgcgg cccccgctac cgaccggaag ccgcccatgc
     2461 gcaccccccg gatcatcgcc gccgccgtcg ccctggcgct gctcggcgcc ccgcccgcac
     2521 acgccgcatt ccacctgatg aaggtggtgg aagtcttccc cggcgcaccc gcggcgccga
     2581 acgcccagta cgtggtgctg cagatgtatt ccggcgggca gaccctcgtc tccgggcacg
     2641 tggtcacggt ctacaacgcc gccggcacgc cggtcggcac ctataccttc ggcgccaacg
     2701 tcgccaatgg cgcggcccag gacaagatcc tgatcgccac gacggaggcg cagaccttct
     2761 tcggcatcac ggcgaacctc atcatgacgc cgaccgttgc cgccgcgggc ggcaaggtct
     2821 gcttcgactc gtcgccaatc gactgcgtgg cctggggcaa ctacagcagc gcgccgaccg
     2881 gcgtgggaac gcccttcaac gatcccgtgc tcggcggcct ggccaccggc cgcgcggcgg
     2941 cacggcggtt ggacatcacc ggcttccccg gcgtgctcga tgccggcgac gataccggcg
     3001 acagcgcgaa cgacttccgg accgccttgc cacgcccgac caacaacgcg cgccagatcg
     3061 gcacgatccc gccctcgacc tgcggcaacc tggcaatcga gggcgtggag cagtgcgacg
     3121 acggtgatct cgacaatggg gatgggtgtt cgagcacctg cgcgatcgag ccgccgctgc
     3181 cgccactgat ctacgcgaac ggattcgagt agcggtcagg gcttttccct cacgtccgaa
     3241 ctcagctgcc agagcaccgg cagacgctgg acgacagcgc cctgcgctgc gacgcgcagt
     3301 tcgaagcgcg agccttcgaa ggccggcgtc tccacgacga tcaccgtccg cgccgcgggt
     3361 cccgcacgct tcctgccgtc cacgaagact tcggtcgctg ccagcaactc gtcgatcgag
     3421 ccgtcgcgaa tcggtgcgcc cgcgtcgtca cggctgatga cgcccttcac gaccggttcg
     3481 ccgaagcggg tcttgccgga ctggcgcaac gggatgccgg tggcttgcag caggcgcacg
     3541 acctccgccc cgaccgggtt gtcgtcgggc tgggtgccct gcgagtagat gtagccgccg
     3601 gcggtcgaga gttcgtcttc gtgcagatcc agcaccaagc gcggcggata atgctgcgcc
     3661 atgcgcaaca cgaattgcgt cagcgcagcg gtctccggcc ccggcgcggc ggcggcgcgt
     3721 ggcccgccgc cctgttcgag atccggcaac aggtactcgg cgtcgccgac gctgtagccg
     3781 cccttcttcc agtcgcgttc ggccgtgttc gggtagcgcc agttgtgccg gtacgcctgc
     3841 ggattggcga gcgggatcac caccatcggc acgccggtgg ccgccagctc ggcgatgatg
     3901 gtgatgtttt ccgcaatggc attgggcccc gcgggttcct cgccatggat gccggacagg
     3961 atccatagcg cctcgccgcg atgggg
//
LOCUS       DAORUK010000176         3926 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_29977_length_3926_cov_19.385947, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000176 DAORUK010000000
VERSION     DAORUK010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29977_length_3926_cov_19.385947"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="PK818_09660"
     CDS             complement(<1..1322)
                     /locus_tag="PK818_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015878151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HQY83035.1"
                     /translation="MKDAFRKPLPGTSLDWFDARAAVEAIRPGAYAALPYTARVHAEN
                     IVRKADPAQRDAYLAQLIERRRDLDFPWFPARVVCHDILGQTALVDLAGLRDAIAAQG
                     GDPAQVNPVVPVQLIVDHSLAVECGGFEPDAFARNRAIEDRRNEDRFHFIEWTRQSFK
                     NVLVIPPGNGIMHQINLEKMSPVVCAEDGMAFPDTCIGTDSHTPHVDALGVIAVGVGG
                     LEAENVMLGRASWMRTPDIVGVELAGRPQPGITATDVVLALTEFLRKEKVVGAYLEFR
                     GEGASALSIGDRATISNMAPEYGATAAMFFIDDNTLDYLRLTGREDAQVQLVETYAKT
                     AGLWADDLASAQYERTLHFDLSSVVRNMAGPSNPHRRLPVSELAARGVADQVKLASGK
                     AEEAGGLIPDGAVIIAAITSCTNTSNPRNVIAAALLARNANAKGLTRKP"
     gene            1403..2404
                     /gene="dusA"
                     /locus_tag="PK818_09665"
     CDS             1403..2404
                     /gene="dusA"
                     /locus_tag="PK818_09665"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HQY83036.1"
                     /translation="MSASPAIPADQLRLSVAPMMDWTDPHCRAFHRLLAPHARLYSEM
                     VHANAVVLGDRARLLAMDPCEHPVALQLGGSEPALLAQAARIGVDAGFDEINLNCGCP
                     SDRVQAGRFGACLMREPALVAESVAAMIAAVPGVPVTVKCRLGVDDDHEWARFLAFID
                     TIADAGCRMFVVHARNAWLKGLSPKENRELPPLRYDWAYRLKRERPALQVIVNGGIAD
                     AVEATAHLDHVDGAMLGRAAYHTPYLLHQLDVRWFGGAARTRGALLRAYRPYVEAQLA
                     RGVYLKHISRHLLGLFAGQPGGRAFRQVLSEGAHQPGADWSLVEHALSLTEREPPKP"
     gene            2401..3312
                     /locus_tag="PK818_09670"
     CDS             2401..3312
                     /locus_tag="PK818_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HQY83037.1"
                     /translation="MITRDLHAFADAFAAAAPDFSAATARAVFLVAPDGFRLAEQSAQ
                     DNSYMADAAAFDATRASAQHRDLQRAVSAVVPAICFAGNVDTPDALFPNNVFGTAPGR
                     YVVGRMRHEVRQREAARDDIRAFFGNVLGRRAIDLSTQPHPCELTGALVIDRARGLGF
                     CGLSERCDEEGARRMHEAFGLRATLLFDLAAGEYHTNVVLAVLAGRAAVVCPKGFADP
                     AVVAAIAALYAPHAVLCSEAEHAAFVGNCIALSPDTAWMSAAAGRALQPAHRDLLAAA
                     GFRVETVELDAIEAAGGSLRCCIGEVY"
     gene            3461..3817
                     /locus_tag="PK818_09675"
     CDS             3461..3817
                     /locus_tag="PK818_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83038.1"
                     /translation="MPKLLTIAILAVLPALSAHAQSQPQPQPRPQRDDGGQDPRSSLS
                     DAVRRVERDTRGEVLSAERVQYDGRDMHRVKVVDDQGRVRVFMQDPQHRDGNGNPPRG
                     DSRGSRRPPPARDDDG"
BASE COUNT          529 a         1414 c         1387 g          596 t
ORIGIN      
        1 ggcttgcggg tcagcccctt cgcgttcgcg ttgcgcgcca gcagcgccgc ggcgatcacg
       61 ttgcgcgggt tgctggtgtt ggtgcagctg gtgatcgcgg cgatgatcac cgcgccatcc
      121 gggatcaggc caccggcttc ctcggccttg ccggaggcga gcttgacctg gtcggcgacg
      181 ccgcgcgcgg ccagctcgct gaccggcaac cggcggtgcg ggttggacgg gccagccatg
      241 ttgcgcacca cggacgacag gtcgaagtgc agcgtgcgct cgtactgcgc gctggcgagg
      301 tcgtccgccc acagcccggc ggtcttcgcg taggtttcga ccagctgcac ctgcgcgtcc
      361 tcgcgcccgg tcaggcgcag gtagtccagg gtgttgtcat cgatgaagaa catcgccgcg
      421 gtcgcgccgt attccggcgc catgttgctg atcgtggcgc ggtcgccgat cgacagcgcg
      481 gaggcgcctt cgccgcggaa ctccaggtag gcaccgacca ccttttcctt gcgcaggaat
      541 tcggtcagtg ccagcaccac gtcggtggcg gtgatccctg gttgcggcct gccggcgagc
      601 tccacgccaa cgatgtccgg cgtgcgcatc cacgaggcgc gcccgagcat gacgttttcc
      661 gcctccagcc cgcccacgcc gaccgcgatc acccccagcg cgtccacgtg cggggtgtgc
      721 gagtcggtgc cgatgcaggt gtccgggaag gccatgccgt cctccgcgca gaccaccggc
      781 gacatcttct ccaggttgat ctggtgcatg atcccgttgc ccggcggaat caccaggacg
      841 ttcttgaacg attgccgggt ccactcgatg aagtggaagc gatcctcgtt gcggcggtcc
      901 tcgatcgcgc ggttcctcgc gaacgcatcc ggctcgaagc cgccgcattc caccgccagc
      961 gagtggtcca cgatcagctg caccggcacc accgggttca cctgcgccgg gtcgccaccc
     1021 tgcgcggcga tcgcatcgcg caggccggca aggtccacca gcgcggtctg gccgaggatg
     1081 tcgtggcaga ccacgcgggc cgggaaccac gggaagtcga ggtcgcggcg gcgttcgatc
     1141 agctgcgcga ggtaggcgtc gcgctgcgct gggtctgcct tgcgcacgat gttctcggca
     1201 tgcacgcggg cggtgtaggg cagcgcggcg tatgcgccgg ggcggatcgc ctcgactgcg
     1261 gcgcgggcat cgaaccagtc gagcgaggtg ccgggcaacg gcttgcggaa ggcgtctttc
     1321 atggcgggcg ggggcgggtg cggcgggatc cgtattgtcg cgcaacccgg ccgggcccgg
     1381 cggctccgta caatgcgcgg ccgtgagtgc atcgcccgcc atccccgccg accagttgcg
     1441 cctgtccgtc gccccgatga tggactggac cgatccgcat tgccgtgcct tccaccggct
     1501 gctggcgccg catgcgcggc tgtattcgga aatggtgcat gccaacgcag tggtgctggg
     1561 cgaccgcgcg cggctgctgg cgatggaccc gtgcgagcac ccggtcgccc tgcagctggg
     1621 cggcagcgag ccggcgctgc tggcgcaggc ggcgcggatc ggcgtcgatg ccggcttcga
     1681 cgagatcaac ctcaactgcg gctgcccgtc cgaccgcgtg caggccggcc gtttcggcgc
     1741 ctgcctgatg cgcgaaccgg cgctggtggc cgagtcggtg gcggcgatga tcgccgcggt
     1801 gccgggcgtg ccggtgacgg tcaagtgccg gctgggcgtc gatgacgacc acgaatgggc
     1861 gcgcttcctc gccttcatcg acacgattgc cgatgccggc tgccggatgt tcgtggtgca
     1921 tgcccgcaat gcctggctga agggcctgag cccgaaggag aaccgcgagc tgccaccgct
     1981 gcgctacgac tgggcctacc ggctcaagcg cgaacgaccg gccctgcagg tcatcgtcaa
     2041 cggcggcatc gccgatgcag tggaagccac cgcgcatctc gatcacgtcg acggcgcgat
     2101 gcttggccgc gcggcctacc acacgcccta cctgctgcat cagctggacg tgcgctggtt
     2161 cggtggcgcg gcccgcacgc gcggcgcact gctgcgcgcg taccgcccct acgtcgaggc
     2221 gcagctggcg cgcggcgtgt acctcaagca catcagccgc cacctgctgg gcctgttcgc
     2281 cggccagccc ggcggccgcg cgttccgcca ggtcctcagc gaaggcgcgc atcaacccgg
     2341 tgccgactgg tcgctggtcg agcacgccct gtccctcacc gaacgcgagc ctcccaaacc
     2401 atgatcaccc gcgacctcca cgccttcgcc gacgccttcg ctgccgccgc cccggacttc
     2461 agcgcggcca ccgcacgcgc ggtgttcctg gtggcgcccg acggcttccg cctcgccgag
     2521 cagtcggcgc aggacaacag ctacatggcc gatgccgcgg cgttcgacgc cacccgtgcc
     2581 tcggcccagc accgcgacct gcagcgcgcg gtgtcggcgg tggtgccggc gatctgcttc
     2641 gccggcaacg tggacacgcc cgatgcgctg ttccccaaca acgtgttcgg caccgcgccg
     2701 ggccgttacg tggtcggccg gatgcgccac gaggtccgcc agcgcgaagc ggcgcgcgac
     2761 gacatccgcg ccttcttcgg caacgtgctg ggccggcggg ccatcgacct gtccacccag
     2821 ccgcacccgt gcgagctgac cggcgcgctg gtgatcgacc gcgcccgcgg gctgggcttc
     2881 tgcggcctgt ccgaacgctg cgacgaggaa ggcgcgcggc gaatgcacga ggcgttcggc
     2941 ctgcgcgcca ccctgctgtt cgacctcgcc gccggcgagt accacaccaa cgtggtgctg
     3001 gcggtgctgg ccgggcgggc ggcggtggtc tgcccgaagg gcttcgccga cccggcggtg
     3061 gtggcggcca tcgccgcgct gtacgccccg catgcggtgc tctgcagcga ggccgaacat
     3121 gccgccttcg tgggcaactg catcgcgttg tcgccggata ccgcgtggat gagcgcggcg
     3181 gccgggcgcg ccttgcagcc cgcgcaccgc gacctgctgg cggcggccgg gttccgggtg
     3241 gagacggtgg aactggatgc aatcgaggcc gccggcggct cgctgcgctg ctgcatcggc
     3301 gaggtctact gatcgcggcc gcgatcgagg tcacggattc gcgtgccggc tgaacggcgc
     3361 ggtatcctgt tcaggaaact tcagggccga ttcaccggct gcgcccagaa tggcgccatc
     3421 ccgtccgagt gccgttgcca tgtcccgctg ccgtccgccc atgccgaagc tgctgaccat
     3481 cgccatcctg gcggtgctgc cggcgctgtc cgcgcatgcg cagtcgcagc cgcagccgca
     3541 gccacggccg cagcgcgacg atggcggcca ggacccgcgc agcagcctgt ccgacgcggt
     3601 gcgccgggtc gagcgcgata cccgtggcga ggtgctgtcc gccgagcgcg tgcagtacga
     3661 cggccgcgac atgcaccggg tcaaggtcgt cgacgaccag ggccgggtgc gcgtgttcat
     3721 gcaggacccg cagcatcgcg acggcaacgg caacccgccg cgcggcgaca gccgcggttc
     3781 gcgccggccg ccgcctgcac gcgacgacga cggctgatcc tgcagcctgg gcgccactgc
     3841 accacccgcg gcatcatttt ccaaaggaga ccccatgcgt atcctgctcg tcgaagacga
     3901 agccccgctg cgcgaaaccc tggccg
//
LOCUS       DAORUK010000177         3887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_30448_length_3887_cov_17.703810, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000177 DAORUK010000000
VERSION     DAORUK010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30448_length_3887_cov_17.703810"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1002
                     /locus_tag="PK818_09680"
     CDS             58..1002
                     /locus_tag="PK818_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HQY83039.1"
                     /translation="MASSFFALFDDIATLLDDISVMTKVATKKTAGVLGDDLALNAQQ
                     VTGIEADRELPVVWAVARGSLLNKAILVPAALAIAALEAWLRGRGFAIPLITPLLMIG
                     GGFLCYEGVEKLAHKFLQSRIDDAHHHAELVHAASDESVDMLAFEKEKIKGAIRTDFI
                     LSAEIIVISLGTVATEPFMRQLGVLVAIALIMTIGVYGLVGGIVKLDDLGLRLAREGA
                     GRARAALGRAILAFAPRLMRFLSIAGTAAMFLVGGGILVHGVPALHHLAEAAQAFPPG
                     WLWENLFNAAAGIAAGAVIVGAVLLVRRLRGKPLTTAH"
     gene            1081..1653
                     /locus_tag="PK818_09685"
     CDS             1081..1653
                     /locus_tag="PK818_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY83040.1"
                     /translation="MAARNRMPPWHENVRLSNGRDVLIRPIRPEDAVPLRAGFALLQP
                     DEVRMRFLHPMKELGAEQAERLTRPNTRREFALVAAEPLPPGEALVGAVARIVVDDDG
                     RAGEFAILVSHYINGMGLGRHLMRRLVRWAKGRKLERIYGDVLESNLPMQALAASLDF
                     HREPGHSPGLVRMVLDLPKPKPRTPPPLVA"
     gene            1732..>3887
                     /locus_tag="PK818_09690"
     CDS             1732..>3887
                     /locus_tag="PK818_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HQY83041.1"
                     /translation="MTHLIDFPTLPLPKPGQLRAYWRVPASASALAVAIAAAAAQHRA
                     PLLLVARDNHAAHQLEADLHTLLGGDPALPVLTFPDWETLPYDRFSPHPDIVSQRLAT
                     LARLPGLQRGIVVVPVATLMQRVAPLAHVIGNRFDFRLGQQLDLDAEKRRLEAAGYRH
                     APQVFDPGDFAVRGGLLDVFPMGAEVPFRIELLDDEIDSIRAFDPESQRSLERIERIE
                     LLPGREVPLDEAPVRRALDALRDRFDIDTRRSALVQDLKAGLAPAGIEYYLPLFHAGT
                     ATLFDYLGSGMLPVLGEGVFEAAEHAWAQIGVRHDQLRHDVERPLLPPAELWLSPDAL
                     REALNRGERIEVCDGAHPRHADAHPLPLQPAPPLPLAAKDQAPAQALRDFLAHYPGRV
                     LVATDSPGRREAMLEMLQAAGLQPEVLADWPAFAGSFTAGRGGLPESLPDTRLVGLPP
                     ASRGEASKFAIAVAPFDDGFALDDPALCVLTERQLFPERASQPRRRKRAGREPEAIIR
                     DLGELSLGAPIVHEDHGVGRYRGLLSMDIGGMPGEFLDIEYAKGDRLYVPVAQLDRIS
                     RYSGANPDTAPLHNLGGEQWTKAKKKAAEKVRDVAAELLEIQARRQARKGLAIDVDRV
                     MYEPFAATFPFEETPDQHAAIEAVIRDLASSQPMDRVVCGDVGFGKTEVAVRAAFVAA
                     AAGKQVAVLVPTTLLAEQHYRNFRDRFADWPLKVEVL"
BASE COUNT          564 a         1444 c         1323 g          556 t
ORIGIN      
        1 gcgccgccgc gacaggccgt atgcgaagat tcgccgcccc cgacaacgcg cattgccatg
       61 gcctccagtt tcttcgccct gttcgacgac atcgccaccc tgctcgacga catctcggtg
      121 atgaccaagg tcgccaccaa gaagaccgcc ggggtgctgg gcgacgacct ggcgctgaac
      181 gcgcagcagg tgaccggcat cgaagccgac cgcgagctgc cggtggtgtg ggcggtggcc
      241 aggggttcgc tgctgaacaa ggcgatcctg gtaccggcgg cgctggcgat cgcggcgctg
      301 gaagcgtggc tgcgcggccg cggcttcgcc attccgctga tcacgccgct gctgatgatc
      361 ggcggcggct tcctctgcta cgagggcgtg gagaagctgg cgcacaagtt cctgcagagc
      421 cggatcgacg acgcccacca ccacgccgag ctggtgcacg cggccagcga cgaatcggtg
      481 gacatgctcg cgttcgagaa ggagaagatc aaaggcgcga tccgcaccga cttcatcctg
      541 tccgcggaaa tcatcgtgat ctcgctgggc acggtcgcca ccgagccgtt catgcgccag
      601 ctgggggtgc tggtcgcgat cgccctgatc atgaccatcg gcgtgtacgg gctggtcggc
      661 ggcatcgtca agctcgacga cctcggcctg cgcctggccc gcgagggcgc cggcagggcc
      721 cgggccgcgc ttggccgcgc gatcctggca ttcgcgccgc ggctgatgcg gttcctctcg
      781 atcgccggca ccgcggcgat gttcctggtc ggcggcggca tcctggtcca cggcgtgccg
      841 gccctgcacc acctggccga ggccgcgcag gccttcccgc ccggctggct ctgggaaaac
      901 ctcttcaatg ccgcggcggg catcgccgcc ggcgcggtca tcgtcggcgc ggtcctgctg
      961 gtgcggcgat tgcgcggaaa gccgctcacc acggcccact gacccggttg cgggcgcccg
     1021 cggcaacgcg cagccatcct gcattcacat ttttaacgtc gatcgggcga tgatccggaa
     1081 atggccgccc gcaaccgcat gcccccgtgg cacgagaacg tccgcctcag caatggccgc
     1141 gacgtcctga tccgcccgat ccgccccgag gacgcggtgc cgctgcgtgc cggcttcgcc
     1201 ctgctgcagc cggacgaagt gcgcatgcgc ttcctgcacc cgatgaagga gctcggcgcc
     1261 gagcaggccg aacgcctgac ccgacccaac acccgccgcg aattcgcgct ggtggccgcc
     1321 gaaccgctgc cgccgggcga ggcgctggtc ggcgcggtgg cacggatcgt ggtggacgat
     1381 gacggccgcg ccggcgaatt cgcgatcctg gtcagccact acatcaacgg catgggcctg
     1441 ggccgccacc tgatgcggcg gctggtgcgc tgggccaagg gccgcaagct ggagcgcatc
     1501 tacggcgacg tgctggagag caacctgccg atgcaggcac tggcggcctc gctggatttc
     1561 caccgggaac ccggccattc gcccgggctg gtgcggatgg tgctggacct gccgaagccg
     1621 aagccgcgca ccccgccacc cctggtcgcc tgagcgccgg cagcggcgcg ctagactgcg
     1681 caccccctgc cgggcacgcc atgtgcccgg caccgccgtg cattggtgcc gatgacccac
     1741 ctgatcgatt tcccgaccct gccgctgccg aagcccggcc agttgcgcgc gtactggcga
     1801 gtgccagcct cggccagtgc gctggcggtg gcgatcgccg cggccgccgc ccagcatcgc
     1861 gcaccgctgc tgctggtcgc gcgcgacaac cacgccgcgc accagctgga agccgacctg
     1921 cacaccctgc tcggcggcga ccccgcgctg ccggtgctga ccttccccga ttgggaaacc
     1981 ctgccctacg accgtttcag cccgcatccg gacatcgtca gccagcgcct ggcgaccctg
     2041 gcccgcctgc ccggactgca gcgcgggatc gtggtggtgc cggtcgccac cctgatgcag
     2101 cgcgtcgccc cgcttgccca cgtgatcggc aaccgcttcg atttccgcct cggccagcag
     2161 ctcgacctgg acgccgagaa gcgccgcctc gaagccgccg gctaccggca cgcgccgcag
     2221 gtgttcgacc ccggcgactt cgcggtgcgc ggcggcctgc tcgacgtgtt cccgatgggc
     2281 gccgaggtgc cgttccggat cgagctgctc gacgacgaaa tcgattcgat ccgtgcgttc
     2341 gacccggaat cgcagcgctc gctggaacgc atcgaacgca tcgaactgct gcccgggcgc
     2401 gaggtcccgc tggacgaggc cccggtcagg cgcgcgctgg acgcactgcg cgaccgcttc
     2461 gacatcgata cccgccgcag cgcgctggtg caggacctga aggcgggcct ggccccggcc
     2521 ggcatcgagt actacctgcc gctgttccac gccggcaccg cgaccctgtt cgactacctc
     2581 ggcagcggca tgctgccggt gctcggcgag ggcgtgttcg aggccgccga acacgcctgg
     2641 gcgcagatcg gcgtgcgcca tgaccagttg cgccacgacg tcgaacgccc gctgctgccg
     2701 cccgccgaac tgtggctgtc gccggacgcg ctgcgcgaag cactcaaccg tggcgagcgc
     2761 atcgaggtct gtgatggcgc ccatccgcgc cacgccgacg cccacccgct gccgctgcaa
     2821 cccgcgccac cgctgccgct ggcggccaag gaccaggccc cggcgcaggc gttgcgcgac
     2881 ttcctggcgc actaccccgg ccgcgtgctg gtcgccaccg attcgcccgg ccggcgcgag
     2941 gcgatgctgg aaatgctgca ggcggcgggt ttgcagcccg aggtgctcgc ggactggccg
     3001 gcgttcgccg gaagcttcac cgcggggcgc ggggggctgc ccgagtcgct ccccgatact
     3061 cgcttggtcg gtctgccccc tgcgtcccgc ggcgaggcca gcaagttcgc catcgcggtg
     3121 gccccgttcg acgacggctt cgcgctcgat gatcccgccc tgtgcgtgct caccgaacgc
     3181 cagctgttcc ccgaacgcgc ctcgcaaccg cgccgccgca agcgcgccgg ccgcgaaccc
     3241 gaggcgatca tccgcgacct cggcgagctc tcgctgggcg caccgatcgt gcacgaagac
     3301 cacggcgtcg gccgctaccg cgggctgctg tcgatggaca tcggcggcat gcccggcgag
     3361 ttcctcgaca tcgaatacgc caagggcgac cgcctgtacg tgccggtcgc gcaactggac
     3421 cggatcagcc gctattccgg cgccaacccg gacaccgcgc cgctgcacaa cctcggtggc
     3481 gagcagtgga cgaaggcgaa gaagaaggca gccgagaagg tccgcgacgt cgccgccgaa
     3541 ctgctggaga tccaggccag gcgccaggcg cgcaagggcc tggcgatcga tgtcgaccgg
     3601 gtgatgtacg aaccgttcgc cgcgaccttc ccgttcgagg aaaccccgga ccagcacgcc
     3661 gcgatcgaag cggtgatccg cgacctcgcc agctcgcagc cgatggaccg ggtggtctgc
     3721 ggcgacgtcg gtttcggcaa gaccgaagtc gcggtgcgcg ccgcgttcgt cgccgccgcg
     3781 gccggcaagc aggtcgcggt gctggtgccg accaccctgc tcgccgaaca gcactaccgc
     3841 aacttccgcg accgcttcgc cgactggccg ctgaaggtgg aagtgct
//
LOCUS       DAORUK010000178         3867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_30711_length_3867_cov_19.035152, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000178 DAORUK010000000
VERSION     DAORUK010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30711_length_3867_cov_19.035152"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2128)
                     /locus_tag="PK818_09695"
     CDS             complement(<1..2128)
                     /locus_tag="PK818_09695"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HQY83042.1"
                     /translation="MSDTPSNDELKQAALDYHRLEPRGKIKVAATKPMVTQRDLALAY
                     SPGVAYACEAIVADPNEVSSLTARGNLVGVITNGTAVLGLGDIGALAGKPVMEGKGVL
                     FQKFAGIDVFDIELNERDPDKLVDIIAAMEPTFGGINLEDIKAPECFIVERKLRERMN
                     IPVFHDDQHGTAIIVGAAVLNALEVAGKKIEEVRLATSGAGAAGIACLDMLVALGMKP
                     EHILAVDRDGVLYTGREHLDPDKQRYARDTDKRSLAEIVAGADIFLGLSAGGVLKPEM
                     VAAMAERPIILALANPYPEILPEDARAVRPDCIIATGRSDYPNQVNNALCFPYIFRGA
                     LDAGATEINEAMKLACVRAIAALARKEASDLGSAYGGEVPKFGAEYLIPRPFDPRLLT
                     MLAPAVAQAAMDSGIAQRPIADLDAYRETLAQFIYRTSLMMKPVYERARKDKKRVAYA
                     EGEEHVVLQAVQTVIDEGLAFPILIGRPGVIENRIAKLGLRMKAGVDFELTNIGADPR
                     FDDYWRQYHALTERRGVTPSAARNLLRSRPTIIAALMVERGEADAMITGIVGRFHKKL
                     GYLRSVFDFEPGVTGTAAMTGVINDHGAWFFVDTHVQLDPTAEQIAEATLMASYRLKL
                     FGIEPKVALLSHSNFGSHQDASALKMRRAFEIIRARMPKLEIDGEMMADTAWDEALRQ
                     RIFPNTRLKGRANLLVMPNLDAANIGY"
     gene            2203..3396
                     /gene="gltS"
                     /locus_tag="PK818_09700"
     CDS             2203..3396
                     /gene="gltS"
                     /locus_tag="PK818_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HQY83043.1"
                     /translation="MLQLDAVQTLALAGLALFLGYGLCRVVPFLARYNLPPPVVGGLV
                     FALAAWAAHGRGTTLFSLDTALQGPLMVAFFTTIGINASLWLLKISGRQVLVFLLLAS
                     GFAIVQNLLGIAVAMGFGLHPLFGVLAGSTTLTGGPATGLAFAPLFEQAGVAGAESIA
                     VASAMAGIVCGGLIGGPVATVLIRRYQLRSTHPEDHRAPPEERSVDVATESQREHAAL
                     KSIVVLLVAMWLGASVSSWISATGVTLPAYVGAMLVGAAVRNIDDRTGWIRLSVPTTD
                     LVGNVCLALFLSVALMNLKLWELAGLALPLLVNLGLQALLVSVFCLLVLRAMGRDYDA
                     AVMSGGFIGFMLGTTANAMAVMRTLVERYGVAPRAFLVAPLVGAFFIDFSNALIITGF
                     MNWLD"
     gene            complement(3406..>3867)
                     /locus_tag="PK818_09705"
     CDS             complement(3406..>3867)
                     /locus_tag="PK818_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1704 domain-containing protein"
                     /protein_id="HQY83044.1"
                     /translation="MKRVSLRIEAVAMALDGADFLQVFRYFLDAGQNEVESFTSAQRV
                     FRGVPTTGGAAFTKDTVYLRGLIGVHTFFRWALKHRKLRLCRLLFAGKMTLADVQRFE
                     PLFDSGDLAPPRWLPHWVERANGLAGMLAFSLFANRIRLEQIVDAESVSDL"
BASE COUNT          500 a         1337 c         1359 g          671 t
ORIGIN      
        1 tgtagccgat gttggcggcg tccaggttcg gcatcaccag caggttggcg cggcccttga
       61 ggcgggtgtt ggggaagatc cgctggcgca gcgcctcgtc ccaggcggtg tccgccatca
      121 tctcgccgtc gatctccagc ttcggcatgc gcgcgcggat gatctcgaag gcgcggcgca
      181 tcttcagcgc gctggcgtcc tggtggctgc cgaagttgga atgcgacagc agcgccacct
      241 tcggctcgat cccgaacagc ttcagccggt agctggccat cagggtggcc tcggcgatct
      301 gttcggcggt cgggtccagc tgcacgtggg tgtcgacgaa gaaccaggcg ccgtggtcgt
      361 tgatcacccc ggtcatcgcc gcggtgccgg tcacccccgg ctcgaagtcg aacacgctgc
      421 gcaggtagcc cagcttcttg tggaagcggc cgacgatgcc ggtgatcatc gcgtccgcct
      481 cgccgcgctc gaccatcagc gcggcgatga tggtgggccg cgagcgcagc aggttgcggg
      541 cggcgctggg ggtcaccccg cggcgctcgg tgagggcgtg gtactggcgc cagtagtcgt
      601 cgaagcgcgg atcggcgccg atgttggtca gctcgaaatc gaccccggcc ttcatccgca
      661 ggccgagctt ggcgatccgg ttctcgatca cccctggccg cccgatcagg atcgggaagg
      721 ccaggccttc gtcgatcacc gtctgcaccg cctgcaggac cacgtgttcc tcgccctcgg
      781 cgtaggccac gcgcttcttg tccttgcgcg cgcgctcgta caccggcttc atcatcaggc
      841 tggtgcggta gatgaactgc gccagggtct cgcggtaggc gtcgaggtcg gcgatcggcc
      901 gctgggcgat gccggaatcc atcgccgcct gcgccaccgc cggcgccagc atggtcagca
      961 ggcgcgggtc gaacgggcgc gggatcaggt actcggcgcc gaacttcggc acctcgccgc
     1021 cgtaggcgct gccgaggtcg gaggcttcct tgcgcgccag cgcggcgatc gcgcgcacgc
     1081 aggccagctt catcgcctcg ttgatctcgg tggcgcccgc gtccagcgcg ccgcggaaga
     1141 tgtacgggaa gcacagcgcg ttgttgacct ggttcgggta gtcgctgcgg ccggtggcga
     1201 tgatgcagtc cgggcgcacc gccctggcgt cctccggcag gatctccggg tacgggttgg
     1261 ccagcgccag gatgatcggg cgttcggcca tcgccgccac catttccggc ttcagcacgc
     1321 cgccggcgga caggcccagg aagatgtcgg cgccggccac gatctcggcg aggctgcgct
     1381 tgtcggtgtc gcgcgcgtag cgctgcttgt ccgggtccag gtgttcgcgg ccggtgtaca
     1441 gcacgccgtc gcggtccacc gccaggatgt gttccggctt catgcccagc gccaccagca
     1501 tgtccaggca ggcgatgccg gccgcgccgg cgccggaggt cgccagcctc acctcttcga
     1561 tcttcttgcc ggccacttcc agcgcgttca gcaccgccgc accgacgatg atcgcggtgc
     1621 cgtgctggtc gtcgtggaac accgggatgt tcatccgctc gcgcagcttg cgctcgacga
     1681 tgaagcactc cggcgccttg atgtcttcca ggttgatgcc gccgaaggtc ggctccatcg
     1741 cggcgatgat gtcgaccagc ttgtcggggt cgcgctcgtt gagttcgatg tcgaacacgt
     1801 cgatcccggc gaacttctgg aacagcacgc ccttgccttc catgaccggt ttgccggcca
     1861 gcgcgccgat gtcgcccagc ccgagcaccg cggtgccgtt ggtgatcacc ccgaccaggt
     1921 tgccgcgcgc ggtcaggctg ctgacttcgt tgggatcggc cacgatcgcc tcgcaggcgt
     1981 aggccacgcc cggcgaatag gcgagcgcga ggtcgcgctg ggtgaccatc ggcttggtcg
     2041 cggcgacctt gatcttgccg cgcggctcca gccggtggta atcgagtgcc gcctgcttga
     2101 gctcgtcgtt gctgggggtg tcggacatct ggacggtccc ggggatgcga atgggtgatg
     2161 ataccggctc cgcccgcgtg gcccggcccg ggacccttgc cgatgctgca actcgacgcc
     2221 gtccagaccc tcgccctggc cgggctggcg ctgttcctgg gctacggcct gtgccgcgtg
     2281 gtcccgttcc tggcccgcta caacctgccg ccgccggtgg tcggcgggct ggtgttcgcg
     2341 ctggccgcat gggccgcgca cggccgcggc accaccctgt tcagcctcga caccgccctg
     2401 caggggccgc tgatggtcgc gttcttcacc accatcggca tcaatgccag cctgtggctg
     2461 ctgaagatca gcggccgcca ggtgctggtg ttcctgctgc tggccagcgg cttcgcgatc
     2521 gtgcagaacc tgctggggat cgcggtggcg atggggttcg gcctgcaccc gctgttcggc
     2581 gtgctggccg gttccaccac cctgaccggc ggcccggcca ccggcctggc cttcgcgccg
     2641 ctgttcgagc aggccggggt ggccggcgcg gagtcgatcg cggtggcctc ggcgatggcc
     2701 gggatcgtct gcggcgggct gatcggcggg ccggtggcga cggtgctgat ccgccgctac
     2761 cagctgcgct ccacccaccc cgaggaccac cgcgcgccgc ccgaggaacg cagcgtggac
     2821 gtcgccaccg aatcgcagcg cgagcatgcc gcgctgaaga gcatcgtggt gctgctggtg
     2881 gcgatgtggc tgggcgcctc ggtcagcagc tggatctccg ccaccggcgt gaccctgccc
     2941 gcgtacgtgg gcgcgatgct ggtcggcgcg gcggtccgca acatcgacga ccgcaccggc
     3001 tggatccggc tgtcggtgcc gaccaccgac ctggtcggca acgtgtgcct ggcgctgttc
     3061 ctgtcggtgg cgctgatgaa cctcaagctg tgggagctgg ccgggcttgc gctgccgttg
     3121 ctggtcaacc tgggcctgca ggcgctgctg gtgtcggtgt tctgcctgct ggtgctgcgc
     3181 gcgatgggcc gcgactacga cgccgcggtg atgagcggcg gcttcatcgg cttcatgctc
     3241 ggcaccaccg ccaacgcgat ggcggtgatg cgcaccctgg tcgaacgcta cggcgttgcc
     3301 ccgcgcgcgt tcctggtggc gccgctggtg ggtgcgttct tcatcgactt cagcaacgcg
     3361 ctgatcatca ccggcttcat gaactggctg gactgaccgc gcgcctcaca ggtcggacac
     3421 gctttccgca tccacgatct gctccagccg gatgcggttg gcgaacagcg agaacgccag
     3481 catcccggcc agcccgttgg cgcgctccac ccagtgcggc agccagcgcg gcggggccag
     3541 gtcgccgctg tcgaacagcg gctcgaagcg ctgcacgtcg gccagggtca tcttgccggc
     3601 gaacagcagc cggcacagcc gcagcttgcg gtgcttgagc gcccagcgga agaaggtgtg
     3661 cacgccgatc agcccgcgca ggtacacggt gtccttggtg aacgccgcgc cgccggtggt
     3721 cggcaccccg cggaacaccc gctgcgcgga ggtgaagctc tcgacctcgt tctggccggc
     3781 gtccaggaag tagcggaaca cctgcaggaa gtccgcgccg tccagcgcca tcgccaccgc
     3841 ctcgatccgc aggctgaccc gcttcat
//
LOCUS       DAORUK010000179         3804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_31568_length_3804_cov_18.581222, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000179 DAORUK010000000
VERSION     DAORUK010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31568_length_3804_cov_18.581222"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="PK818_09710"
     CDS             complement(<1..736)
                     /locus_tag="PK818_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005914752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HQY83045.1"
                     /translation="MDIGYFLKLMAEKNASDMFLSSGAPVNIKVEGELLPLGSAPLPA
                     GMPKKVAYSLMSEAQIAEFERELEMNMAIAVAGSGRFRVNVFQQRGEVGLVIRAIRSD
                     IPTIEELQLPQVLKSIIMAPRGLVLIVGSTGAGKSTTLASMIDHRNSTTSGHILTIED
                     PIEYLHRHKRSIVNQREVGLDTHSFHAALKNAMREAPDVILIGEIRDSTTMEAAIAFA
                     ETGHICLATLHSNNADQTIERILNFFP"
     gene            824..2086
                     /locus_tag="PK818_09715"
     CDS             824..2086
                     /locus_tag="PK818_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQY83046.1"
                     /translation="MRPSFAHFPVTRHALLCGLAFAFALAGCATLPPPTAELDAAQQA
                     VARADAADGDQYAAAPLAQARAGLERAQAAMARGRDEEARSAALAASADADLSHVLSR
                     AAVTRAEFEQQQAQLAALKGRLGMDAIVVQDNPLDLPTVAAPAAQRLQALDADTRLNP
                     FAQYERLQARQALAAAQAAGKRERAGAEQLAERRVGIAEQAARIEAARRAIEDMERER
                     SELLVEASRRDAERARAEAERLRIEAQVQAEEAARLRAQAEQAEAVLDTARVDQDARV
                     AAARAKEAALARQEAELVAGAALPPSRKQADGEVFTLAGDAFGSGQAQLTADAAASVR
                     ALGLYLKAIGAGSVQVLGHTDSQGEAEANRALSQRRADAVQAALVAAGIGRARTRADG
                     RGEDAPVADNLTAAGRAQNRRVEIVVAN"
     gene            2499..2714
                     /locus_tag="PK818_09720"
     CDS             2499..2714
                     /locus_tag="PK818_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HQY83047.1"
                     /translation="MFEGQPQAELEAMMKASPEFRQLYYRHKELDKQVLDAELGVLPL
                     DDTTLGQMKREKLAAKDRLTRMYESTH"
     gene            2846..>3804
                     /locus_tag="PK818_09725"
     CDS             2846..>3804
                     /locus_tag="PK818_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HQY83048.1"
                     /translation="MPIHDSILDLVGDTPIIRARRLDTGPCTLYLKLESQNPGGSIKD
                     RIGLSMIEAAEKRGDIRPGDTLVEGTAGNTGIGLALVAQQKGYKLILVVPDKMSREKI
                     FNLKAMGAQVVLTRSDVAKGHPDYYQDLAKAIADRTPGAYFINQFGNQDNPAAHESGT
                     GPEILRQMAGVGGLDAIVFGCGSSGTMTGLSRCFAAHSPHTELVLADPVGSILEEYIN
                     RGTLSDKSASWMVEGIGEDFLPPISDFTRVKQAYAISDKESFLTARELLEKEGILGGS
                     SSGTLLAAALKYCREQTTPKNVLVFVCDTGNKYLSKMYNDYWML"
BASE COUNT          618 a         1301 c         1339 g          546 t
ORIGIN      
        1 cggggaagaa gttgaggatg cgctcgatgg tctggtcggc gttgttggag tgcagcgtgg
       61 cgaggcagat gtggccggtc tccgcgaacg cgatcgccgc ctccatcgtc gtcgaatcgc
      121 ggatttcgcc gatcaggatc acgtccggcg cctcgcgcat ggcgttcttc agcgcggcgt
      181 ggaagctgtg ggtgtcgagc ccgacctcgc gctggttgac gatcgagcgc ttgtgccggt
      241 gcaggtattc gatcgggtcc tcgatggtga ggatgtggcc ggaggtggtg ctgttgcgat
      301 ggtcgatcat cgaagccagg gtggtcgact tgccggcgcc ggtggagccg acgatcagca
      361 ccagtccgcg cggcgccatg atgatgctct tcagcacctg cggcagctgc agttcctcga
      421 tggtggggat gtcgctgcgg atcgcgcgga tcaccaggcc gacctcgccg cgctgctgga
      481 acacgttcac gcggaagcgg ccggagccgg ccaccgcgat cgccatgttc atctccagct
      541 cgcgttcgaa ctcggcgatc tgcgcctcgc tcatcagcga ataggcgacc tttttcggca
      601 tccccgccgg cagcggcgcg ctgcccagcg gcagcagctc gccctcgacc ttgatgttca
      661 ccggcgcgcc cgaactcagg aacatgtccg aggcgttctt ttccgccatc agcttgagga
      721 agtagccgat atccatcgcg tcgattcccg ttcgctgccg gcgatttgcg catgcgccgc
      781 cggcttccca caatgaccgc acgccaacac gcacggtccc cctatgcgac caagcttcgc
      841 acacttcccg gtaacgcggc acgcgctcct gtgcggcctt gcgttcgcgt tcgcacttgc
      901 cggctgcgcc accctgccgc ccccgaccgc ggagctggat gccgcgcagc aggcggttgc
      961 gcgggccgat gcggccgatg gcgaccagta cgccgcggcc ccgctcgcgc aggcgcgggc
     1021 gggcctggag cgcgcgcagg cggcgatggc gcgcggtcgc gacgaggaag cacgcagcgc
     1081 cgcgctcgcg gccagcgccg atgcggacct gtcccatgtg ctgagccggg cggcggtgac
     1141 ccgcgccgag ttcgagcagc agcaggcgca gctcgccgcg ctgaaggggc gcctggggat
     1201 ggatgcgatc gtggtccagg acaatccgct ggatctcccc accgtggccg cgccggccgc
     1261 gcagcgcctg caggcgctgg atgcggacac ccggctgaat ccgttcgccc agtacgagcg
     1321 gctgcaggcc cgccaggcac tggcggccgc gcaggccgcc ggcaagcgcg agcgcgccgg
     1381 cgccgagcag ttggccgaac gccgggtcgg gattgccgag caggcggcgc ggatcgaggc
     1441 cgcccgccgc gcgatcgagg acatggagcg cgagcgcagc gagttgctgg tggaagccag
     1501 ccgccgcgac gccgagcgcg cgcgtgccga ggccgagcgc ctgcgcatcg aagcccaggt
     1561 gcaggccgag gaagcggcgc gcctgcgcgc acaggccgaa caggccgagg ccgtgctgga
     1621 caccgcacgg gtcgaccagg atgcccgcgt ggccgccgcg cgtgcgaagg aagcggcctt
     1681 ggccaggcag gaggcggaac tggtcgcggg cgcggccttg ccgccgtcgc gcaagcaggc
     1741 cgacggggag gtcttcaccc ttgccggcga tgccttcggc tccggccagg cccagctcac
     1801 cgcggatgcc gcggccagcg tgcgggccct gggcctgtac ctgaaggcga tcggcgccgg
     1861 cagcgtgcag gtgctcggcc acaccgacag ccagggcgag gccgaggcca accgcgcgct
     1921 gtcgcagcgg cgcgccgacg cggtgcaggc agcgctggtc gcggccggca tcggccgggc
     1981 ccggacccgc gcggacgggc gcggcgagga cgcgccggtg gccgacaacc tgaccgcggc
     2041 ggggcgcgcg cagaaccgcc gggtggagat cgtcgttgcc aattaaccct ttcaaaacat
     2101 tgggttaaca aaagtctgcg ggtacgggca gggggcgcgt gaccgcccgt cgcctgcact
     2161 tgcggatggc cggaggcagg cgtagtgtcg gttacccaga tggttgcgaa cctcccccat
     2221 ctggccggag accggccgat gcttgatccg ctcgacaatt cggagaccgc cgccgcaagc
     2281 caggatcgcg gcggcctgtc cggacgccgt ggccgcatcg cattccacgc tccaacgcat
     2341 tcccgtacgc cccgcgctgc cagcagccgg ggcgtacgtg tatttgcgat ccgcgaaccg
     2401 gcggatcgcg cattccggca ggcccccacc tgccaccacc cgcgccgccc cggcccgtcc
     2461 ggccgggctc cgcatcgttg aaaccgaagg aggtttccat gttcgaagga caaccgcagg
     2521 cggaactcga agcgatgatg aaagccagcc ccgagttccg gcagctgtac taccggcaca
     2581 aggagctgga caagcaggtg ctggacgccg agcttggcgt gctgccgctg gatgacacca
     2641 cccttggcca gatgaagcgc gaaaagctcg ccgccaagga ccgcctgacc cggatgtacg
     2701 aaagcacgca ctgaaacctc gtcggggcgg cgatcggcct gcgcgtgcgg gcgatcgccg
     2761 cccgttccgc gcctgcagcg ccggttccgg gctgcgtggg accggctgct agaatcgcgt
     2821 tcgacgcaat gccagcgggt cccgcatgcc gatccacgat tccatccttg acctggtcgg
     2881 cgataccccg atcatccgtg cgcgtcgcct cgatacgggc ccgtgcacgc tttacctgaa
     2941 gctcgaaagc cagaaccccg gcgggtcgat caaggaccgc atcggcctga gcatgatcga
     3001 ggcggcggag aagcgcggcg acatcaggcc cggcgacacc ctggtggaag gcaccgccgg
     3061 caacaccggc atcggccttg ccctggtggc gcagcagaag ggctacaagc tgatcctggt
     3121 ggtcccggac aagatgagcc gggagaagat cttcaacctg aaggcgatgg gcgcgcaggt
     3181 ggtgctgacc cgcagcgacg tcgccaaggg ccatccggac tattaccagg acctggccaa
     3241 ggccatcgcc gaccgcacgc cgggcgcgta cttcatcaac cagttcggca accaggacaa
     3301 cccggccgcg cacgagtccg ggaccgggcc ggagatcctg cggcagatgg ccggggtcgg
     3361 cgggctggac gccatcgtct tcggttgcgg aagttcgggg accatgaccg gcctgtcgcg
     3421 ctgcttcgcg gcgcattccc cgcacaccga gctggtgctg gcggatccgg tgggctcgat
     3481 cctcgaggaa tacatcaacc gcggcaccct cagcgacaag tccgcgagct ggatggtgga
     3541 aggcatcggc gaggatttcc tgccgccgat ctccgatttc acccgcgtca agcaggccta
     3601 cgcgatcagc gacaaggaaa gcttcctcac cgcgcgcgaa ctgctggaga aggagggcat
     3661 cctcggcggc tcgtcgagcg gcaccctgct ggcggcggcg ctgaagtact gccgcgaaca
     3721 gaccacgccg aagaacgtgc tggtgttcgt ctgcgatacc ggcaacaagt acctgtcgaa
     3781 gatgtacaac gactactgga tgct
//
LOCUS       DAORUK010000180         3779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_31906_length_3779_cov_16.554511, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000180 DAORUK010000000
VERSION     DAORUK010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31906_length_3779_cov_16.554511"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..749
                     /locus_tag="PK818_09730"
                     /pseudo
     CDS             1..749
                     /locus_tag="PK818_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007964080.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(800..889)
                     /locus_tag="PK818_09735"
     tRNA            complement(800..889)
                     /locus_tag="PK818_09735"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(853..855),aa:Ser,seq:tga)
     gene            1117..1770
                     /locus_tag="PK818_09740"
     CDS             1117..1770
                     /locus_tag="PK818_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011408972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQY83049.1"
                     /translation="MGIKVFIVDDHALVRTGFRLILAQHPDIEIVGEADSGEEALPQI
                     RRLKPDVLLCDLHLPGISGLEVTERVVRGDYGTRVIMVSVLEDGPMPRRLLEAGACGY
                     VGKAGDAAELVRAVREVARGKKYLANAIAQGMALSGIGGDASSPFDALTPRELEVALL
                     LNQGLRQEAIAKRLSLSAKTVNTHKSRLFEKLAIRDNIALARLVSQYGLADPAHAMH"
     gene            complement(1797..>3779)
                     /locus_tag="PK818_09745"
     CDS             complement(1797..>3779)
                     /locus_tag="PK818_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HQY83050.1"
                     /translation="RLRDLGGLVVIDFIDMSSNKHQREVENKLQNALKYDRARVQLGR
                     ISRFGLMEMSRQRLRPSLGEASQIVCPTCEGHGRMRSIESLSLSIIRVAEEHAMKDNT
                     GQVLVQAPVEIANFLLNEKRSALREIEARHDAPIVIVADEQLHTPHYEVTRIRENELG
                     EETARPSYQRGTPRKVATISLTKAQLNIPPAPVVTNVKPAQPAPLREPRPMPVESEAR
                     AVAVAEPTPAAAPVAAPGGGFMGWLKSMFAGAPAVQAPPAIAGNQGGPREERGNRRDR
                     GDRGDRGDRGNRRDEGRRNGGQQDNRGGKPADAQKPQAPKPPKPQQAQARQDNKPQQQ
                     AQKPQAAQKQQQQKPDRQEKAQPVKPAVAEAAVVAAAATPAVAEDAILNTVATVEQAA
                     ATVATPPQAIEADAAADDGTGDGGSKRRRGRRGGRRRRRGAGEAAGMADEAMLDQDGP
                     LDAALATDGSQPEFDFDDIEAPAPVAVVAREAAPAPAAVAAAASAVASEPVTAEVPSP
                     EVAAVAPIESAAAVVETVAADADPVIEAIAVVAEHAEAAPIEPVAPSQATTPQAPVEA
                     EAVANLFEALPADAAEDVAPAPAITAEVVATPAIAEIVAPLPAAANAAGASPVVADAV
                     SRAEQLRGLFDNARQEPANAPLPPADDAAAETSRNA"
BASE COUNT          499 a         1301 c         1352 g          627 t
ORIGIN      
        1 cggtcaccga cgtcggcacc agcgtgcatc gccacgcgca gtcgatgctg gccgaagcgc
       61 aggccgcgcg tgaagtggtg gaccgcctca gcgcggaacc gcgcggcacc gtgcgggtca
      121 gcctgccggt gtcgatggcg caacagcagt tcccgaaact gctgcccgaa ttcatggcgc
      181 tgtacccgaa ggtgcgggtg caactggtca tcagcaaccg ccgggtcgac gtgatcaacg
      241 agggcgtgga cgtggccctg cgcgtccgca ccaagctcga cgatgacggc agcctggtga
      301 tgcgcagctt cggccaggtg caggagctgc tggtcgccag cccgagctac ctcgaccgcg
      361 ccggccgccc gcaggcgccg gaggaattgc gcgaccacgt caccctgagc atcagcgagg
      421 acgaggcgcg ccagcgctgg gaactgcacg gcccggacgg cgaggtgcgg cggatcgaat
      481 tgcagccgcg cgtcaccggg ttcgacttcc cgatgatgca ggcgatggcg gagggtggca
      541 tcggcatcac cctgctgccg gaaaccgtct gcgccgacgc ggtgcgccgg ggcgacctcg
      601 aagtggtgct gccggactgg cggctgccgc agggcatcgc ccacgcggtg ttcgcctccc
      661 gtcgcggcct gctcccggcg gtgcgggtgt tcatcgactt cctggccgag cgcctgcctg
      721 cgctgctgga cgccgcacgc ctggaatgca gcgagaaggg ttgccatggc aacggcacga
      781 agcccaggaa ggcatgatct ggcggaagcg gtgagattcg aactcacgga tggtttgacc
      841 catcgccggt tttcaagacc ggtgccttaa accgctcggc cacgcttccg tggcgcagat
      901 tgtcgcatgg gcattgagtt cgacaaatcg gcaggattgc cgatttggac agccgcaggc
      961 tgcccgcgca gcgggtgagg gccatggatg gcccgaatct atcccactct ctccgccacc
     1021 ctcgtaggac ttttcctact gcctggtgcg gcattcccgg cttggcgacg gtcggtggcg
     1081 ggcgtagcgt gtccccatca atcgggggga gctgcaatgg gcatcaaggt tttcatcgtc
     1141 gacgaccacg cgctggtgcg caccgggttc aggctgatcc tggcccagca cccggacatc
     1201 gagattgtcg gcgaagcgga cagcggcgaa gaggcgttgc cgcagatccg ccggttgaag
     1261 ccggatgtgt tgctgtgcga cctgcacctg cccgggatca gcggactgga ggtgaccgaa
     1321 cgcgtcgtcc gcggcgacta cggcacccgc gtgatcatgg tctcggtgct ggaggacggg
     1381 ccgatgccgc ggcgcttgct ggaggcgggg gcctgcggtt acgtcggcaa ggccggcgac
     1441 gcggcggaac tcgtgcgcgc ggtgcgcgag gtcgcccgtg gcaagaaata cctggcgaat
     1501 gcgatcgcgc agggcatggc gctgtccggg atcggcggtg acgccagttc gccgttcgac
     1561 gcgctgaccc cgcgcgagct ggaagtcgcg ttgctgctca accagggcct gcgccaggaa
     1621 gcaatcgcca agcgcctcag cctcagcgcc aagaccgtca acacccacaa gtcgcggctg
     1681 ttcgagaagc tggcgatccg cgacaacatc gccttggcgc ggttggtcag ccagtacgga
     1741 ctggccgatc ccgcgcacgc catgcactga cggcgcgcgc ggtcggcggc tcgcggtcag
     1801 gcgttgcggc tggtttccgc ggccgcgtca tccgccggcg gcaacggcgc gttggcgggt
     1861 tcctggcgcg cattgtcgaa cagcccgcgc agctgctcgg cacggctcac ggcgtccgcg
     1921 accaccggcg aagcgcctgc ggcattcgcg gcggccggca atggagccac gatttccgca
     1981 atggctggcg tcgccaccac ttccgcggta atggcaggcg ccggggcgac gtcctctgca
     2041 gcatcggcgg gcagcgcctc gaagaggttg gcgaccgctt ctgcttcgac cggcgcctgc
     2101 ggcgtggttg cctggctggg cgcgacgggc tcgatgggag ctgcctccgc gtgctcggca
     2161 accaccgcga ttgcctcgat gaccggatcg gcgtccgcgg ccacggtttc gacgaccgcg
     2221 gctgccgatt caatcggcgc gaccgctgca acctcgggtg acggcacctc tgcggtgacc
     2281 ggctcgctgg cgacggccga agccgccgcc gcgactgcgg cgggggcagg ggcggcttcg
     2341 cgcgccacca ccgctaccgg agccggcgct tcgatatcgt cgaaatcgaa ttccggctgc
     2401 gagccatcgg tggccagcgc ggcgtccagc ggaccatcct ggtccagcat tgcctcatcg
     2461 gccatgccgg cggcttcacc ggcaccgcgg cggcggcggc ggccaccgcg gcgaccgcgg
     2521 cgacgcttgg agccaccatc gccggtgcca tcgtcggcag cggcgtcggc ttcgatcgcc
     2581 tgcggcggcg tcgccaccgt tgccgccgcc tgctcgacgg ttgcgaccgt gttgaggatg
     2641 gcgtcctcgg ccacggcggg cgttgccgcg gcggcgacga cggcggcttc cgccacggcc
     2701 ggcttcaccg gctgggcctt ctcctgccgg tccggcttct gctgctgttg cttctgcgcc
     2761 gcttgcggct tctgcgcctg ctgctgcggc ttgttgtcct gccttgcctg cgcctgctgc
     2821 ggcttcggcg gcttcggcgc ctgcggcttc tgcgcatccg ccggcttgcc gccacggttg
     2881 tcctgctgcc cgccgttgcg gcggccctcg tcacgccggt tgccgcgatc accacggtcg
     2941 ccacggtcac cgcggtcgcg acggttgcca cgttcctcgc gggggccgcc ctggttgccg
     3001 gcgatggccg gtggtgcctg caccgcgggc gcgccggcga acatcgactt cagccagccc
     3061 atgaagccgc cgccgggggc cgccaccggc gccgctgcgg gcgtcggctc ggccaccgca
     3121 accgcacgcg cttcgctttc gaccggcatc ggccggggct cgcgcagcgg cgccggctgc
     3181 gccggcttga cgttggtcac caccggtgcc ggcgggatgt tgagctgggc cttggtcagc
     3241 gagatggtcg ccaccttgcg cggggtgccg cgctggtagc tcgggcgcgc ggtttcctcg
     3301 cccagttcgt tctcgcggat gcgggtgact tcgtagtgcg gggtgtgcag ctgttcgtcg
     3361 gccacgatca cgatcggcgc gtcgtgccgc gcctcgatct cgcgcagcgc gctgcgcttc
     3421 tcgttgagca ggaagttggc gatctccacc ggcgcctgca ccagcacctg gccggtgttg
     3481 tccttcatcg cgtgctcttc ggccacgcgg atgatcgaca gcgacagcga ttcgatgctg
     3541 cgcatgcgac catggccttc gcaggtcggg cagacgatct ggctggcttc gcccagcgac
     3601 gggcgcagcc gctggcggct catttccatc aggccgaagc gcgagatgcg gcccagctgc
     3661 acgcgcgcgc ggtcgtactt gagcgcgttc tgcagcttgt tctcgacttc gcgctggtgc
     3721 ttgttgctgg acatgtcgat gaagtcgatc accaccagcc cgccgaggtc gcgcaggcg
//
LOCUS       DAORUK010000181         3741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_32410_length_3741_cov_17.396365, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000181 DAORUK010000000
VERSION     DAORUK010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32410_length_3741_cov_17.396365"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PK818_09750"
     CDS             complement(<1..182)
                     /locus_tag="PK818_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83051.1"
                     /translation="MSATDATATKLSATWLTRWDPEDQAFWAGGGSTIAWRTLALTTV
                     NLTLAFAAWFMVSALVV"
     gene            complement(377..1789)
                     /locus_tag="PK818_09755"
     CDS             complement(377..1789)
                     /locus_tag="PK818_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQY83052.1"
                     /translation="MGAAFLQALRACLGDPNVLVDGDLDAFEVDWRKRYRGRALAVAR
                     PSCTAEVAAVVRLCASHGVPIVPQGGNTGLVGGGVPDASGRQLVLSLARMARVRAVDA
                     DNLTATVEAGCVLQDVQAAATAHGLLFPLRLGSEGSCTIGGNLASNAGGTQVLRYGNA
                     RELCLGLEVVTAAGEVWDGLSGLRKDNSGYDLRDLFIGSEGTLGIITAATVKLHPRPG
                     ATLTALAACPGIDAVLALLRAARSRLDAGLTGFELIEGFALHLLARHYPDLRQPLDAP
                     AWAVLLEVAAGNETEARERLHALLDEASAHGLVGDAVVAASLAQARELWQLRESIPLA
                     QAREGLNIKHDIALPVSAIPGFLAEVEPRLRAEVPDLRVVVYGHLGDGNLHYNLQAPQ
                     GMPAADFLQHHEASLNRCVYDAVLARGGSFSAEHGIGALKVAEMAARKSPVALALMRT
                     IKQALDPHGLLNPGRVLPPP"
     gene            complement(1794..2678)
                     /locus_tag="PK818_09760"
     CDS             complement(1794..2678)
                     /locus_tag="PK818_09760"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQY83053.1"
                     /translation="MSSRAIPIVMDAGPLSDAAAKATAEAHAHAHADDPSQPLAGPAP
                     VFVMVGDTPIDEAEIAREMQFHRADDPHAARQAAATTLVIRELVRRECERLQLAVEAI
                     DGETEDEARVRVLVDAEVALPEADEAALRQYYEANQARLHHPDAIRARHILLAAAPSD
                     IAARQRAQQHGEELIGALREEPDRFIEFAMRHSACPSREQGGELGWIERGDTVPEFDR
                     QLFMLKPGMAGLTVETRYGHHVVEVLERVDGEPLSFEDARERIAAYLETQAQQNALHQ
                     YLHILAQRHGVEGIALGD"
     gene            complement(2682..3383)
                     /gene="narI"
                     /locus_tag="PK818_09765"
     CDS             complement(2682..3383)
                     /gene="narI"
                     /locus_tag="PK818_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HQY83054.1"
                     /translation="MNYINTFLFQIYPYIALAIFAIGCWARFDHGAYTWRSGSTQLLS
                     GKWMRLGSNWFHVGILAILGGHLVGLLTPHAVYEHVITAPQKQLVAMVVGGLFGVMCF
                     IGLSILLARRLFNPRIRATSSGRDNMILVLLYAQLILGLSSIFVSAGHLDGGQMIKLG
                     EWAQHVVTFRGGAAEFMADAHWIFKAHVSLGMTLILLTPFTRLVHVWSIPVGYLTRPY
                     QVVRKRQRPLQYKAR"
     gene            complement(3380..>3741)
                     /locus_tag="PK818_09770"
     CDS             complement(3380..>3741)
                     /locus_tag="PK818_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006371182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HQY83055.1"
                     /translation="PDYLPVVLEYLSMRPVAEVRDWLGHIGHILELLAARAQERNSTY
                     AALLEALVETASGRVDLDLLRKRVASEERDDTPEAMDRVWEEEAVRFGVEAPGEDCNP
                     PTRLPSGVKRNEPTVTP"
BASE COUNT          526 a         1306 c         1358 g          551 t
ORIGIN      
        1 accaccagcg ccgagaccat gaaccaggcg gcgaaggcga gggtcaggtt gaccgtggtc
       61 agggccagcg tgcgccaggc gatggtggag ccgccgccgg cccagaacgc ctggtcctcg
      121 gggtcccagc gggtcagcca ggtggcggac agcttggtgg cagtggcgtc ggtcgcggac
      181 atggagcctc cggcaggcaa tgggggtggg gatgcaggaa ttggcggcat gatgccggtt
      241 gtccgccggc gtcgatttga cgttcatcaa ttgcgcggcg aatccagctt gattcacgtc
      301 aagcctgccg gaagcgcggt gtcggcgggt cgttcgtgcc ggtgcgggcg cgcccgccac
      361 ggcgggtggc cggcgctcag ggcgggggca gcacccggcc cggattcagc aggccgtgcg
      421 ggtccagcgc ctgcttgatc gtgcgcatca gcgccagcgc caccggcgac ttgcgcgcgg
      481 ccatctcggc caccttcagc gcgccgatgc cgtgctcggc ggaaaacgag ccgccgcgcg
      541 ccagcaccgc gtcgtacacg caacggttga ggctggcctc gtggtgctgc aggaaatcag
      601 cggcgggcat gccctgcggt gcctgcaggt tgtagtgcag gttgccgtcg ccgaggtggc
      661 catagaccac cacccgcagg tccgggactt cggcacgcag ccgcggttcc acctcggcca
      721 ggaagccggg gatcgccgac accggcaacg cgatgtcgtg cttgatgttc aggccttcgc
      781 gcgcttgcgc cagcgggatc gactcgcgca gctgccagag ctcgcgtgcc tgggcgaggc
      841 tggcggcgac gactgcatcg ccgaccaggc cgtgcgcgga tgcttcgtcc agcagtgcgt
      901 gcaggcgttc gcgcgcctcc gtctcgttgc cggcggccac ttccagcagc accgcccacg
      961 ctggcgcgtc cagcggttgc cgcaggtccg ggtagtgccg cgccagcagg tgcagggcga
     1021 agccctcgat cagctcgaag ccggtcaggc cggcatccag gcggctgcgc gcggcgcgca
     1081 gcagggcgag cacggcgtcg atgccggggc aggcggccag cgcagtgagg gtggcgcccg
     1141 ggcgtggatg cagcttgacg gtggcggcgg tgatgatccc cagggtgcct tcgctgccga
     1201 tgaacaggtc gcgcaggtcg tagccgctgt tgtccttgcg caggccggac aggccgtccc
     1261 acacctcgcc ggcggcggtc accacttcca gtcccaggca cagctcgcgc gcgttgccat
     1321 agcgcagcac ctgggtgcca ccggcattgc tggccaggtt gccgccgatc gtgcagctgc
     1381 cctcggagcc cagccgcagc gggaacagca ggccgtgcgc cgtggccgcc gcctgcacgt
     1441 cctgcagcac gcagcctgcc tcgacggtgg cggtcaggtt gtcggcatcg accgcgcgca
     1501 cccgcgccat ccgcgccagg ctcagcacca gctggcggcc gctggcatcc ggcacgccgc
     1561 cgccgaccag gccggtattg ccgccctgcg gcacgatcgg taccccgtgg ctggcgcaca
     1621 ggcggaccac ggcggcgacc tcggcggtgc aggacgggcg tgccaccgcc agcgcgcggc
     1681 cgcggtagcg cttgcgccag tcgacctcga atgcgtcgag gtcgccatcg accagcacgt
     1741 tcggatcgcc gaggcaggcg cgcagcgcct gcaggaacgc cgcgcccatg gggtcagtct
     1801 cccagcgcga tgccttccac gccgtggcgt tgcgccagga tgtgcaggta ctggtgcagc
     1861 gcgttctgct gggcctgggt ttccaggtag gcggcgatcc gctcgcgcgc gtcctcgaac
     1921 gacagcggct cgccgtcgac ccgctccagc acctcgacca cgtggtggcc gtaacgcgtc
     1981 tccaccgtca gccccgccat ccccggcttg agcatgaaca gctggcggtc gaactccggc
     2041 acggtgtcgc cgcgttcgat ccagcccagt tcgccgccct gctcgcgcga ggggcaggcg
     2101 gagtggcgca tcgcgaattc gatgaagcgg tccggctcct cgcgcagcgc gccgatcagc
     2161 tcctcgccat gctgctgcgc gcgctggcgg gcggcgatgt cggacggcgc ggcggccagc
     2221 aggatgtggc gggcgcggat cgcgtccggg tggtgcaggc gggcctggtt ggcctcgtag
     2281 tactggcgca gcgcggcttc gtcggcctcc ggcaacgcca cctcggcgtc gaccagcacc
     2341 cgcacccggg cctcgtcctc ggtttcgccg tcgatcgcct cgaccgccag ctgcaggcgc
     2401 tcgcattcgc gccgcaccag ttcgcggatg accagggtgg tggccgcggc ctggcgcgcc
     2461 gcgtgcgggt cgtcggcgcg atggaactgc atctcgcggg cgatctcggc ctcgtcgatc
     2521 ggggtgtcgc cgaccatcac gaacaccggc gccggacccg ccagcggctg gctgggatcg
     2581 tcggcatgcg cgtgcgcatg cgcctcggcg gtggccttgg ccgccgcatc cgacagcggc
     2641 ccggcatcca tcacgatcgg gatcgcgcgg ctggacatcg gtcagcgcgc cttgtactgc
     2701 agcgggcgct ggcgcttgcg caccacctgg tacggacggg tcaggtagcc gaccgggatc
     2761 gaccacacgt gcaccagccg ggtgaacggc gtgagcagga tcagggtcat gcccagcgac
     2821 acgtgcgcct tgaagatcca gtgggcgtcg gccatgaact cggcggcgcc gccgcggaag
     2881 gtgacgacgt gctgcgccca ctcgcccagc ttgatcatct ggccgccgtc caggtgcccg
     2941 gcggacacga agatcgagct caggcccagg atcagctgcg cgtacagcag gaccaggatc
     3001 atgttgtcgc ggccgctgga ggtggcgcgg atgcgcgggt tgaacaggcg ccgggccagc
     3061 aggatgctca ggccgatgaa gcacatcacg ccgaacaggc cgcccaccac catcgccacc
     3121 agttgcttct gcggcgcggt gatgacgtgc tcgtacaccg cgtgcggggt cagcaggccg
     3181 accaggtggc cgcccaggat cgccaggatg ccgacgtgga accagttgct gccgagccgc
     3241 atccacttgc cggacagcag ctgggtggaa ccgctgcgcc aggtgtacgc gccgtggtcg
     3301 aagcgcgccc agcagccgat cgcgaagatc gccagcgcga tgtagggata gatctggaac
     3361 aggaaggtgt tgatgtagtt catggcgtca ccgtgggttc gttccgcttc acgcccgacg
     3421 gaagccgggt aggggggttg cagtcttcgc cgggggcttc gacaccgaag cgcaccgctt
     3481 cctcttccca gacgcggtcc atcgcctccg gggtgtcgtc gcgctcctcg ctggcgaccc
     3541 gcttgcgcag caggtcgagg tcgacccggc cgctcgcggt ctccaccagc gcctccagca
     3601 gcgccgcgta cgtgctgttg cgctcctgcg cgcgcgcggc cagcagttcg aggatgtggc
     3661 cgatgtggcc gagccagtcg cgcacttccg cgaccggccg catcgacagg tattccagca
     3721 ccaccggcag gtagtccggc a
//
LOCUS       DAORUK010000182         3698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_32981_length_3698_cov_19.147955, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000182 DAORUK010000000
VERSION     DAORUK010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32981_length_3698_cov_19.147955"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /gene="rhlB"
                     /locus_tag="PK818_09775"
     CDS             <1..659
                     /gene="rhlB"
                     /locus_tag="PK818_09775"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:NF023720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HQY83056.1"
                     /translation="AEGDAISFACERYGMSLPDIEAFIDQKIPGEHVTPEMLVALPRK
                     PREGVEAQPEDGESIGEIFKEVREARAAEDAKRGGGRGGPGGRGERSGPRSGERRPGG
                     SDSRGPRPPRKPRVEGEVAASPAVAAAPAAVAPAAAVVADGERPQRKPRRRRGGKRLD
                     QGTVATPPQAGAGTASPAVAPPAAGPAAAGAPAAQASLLARIGRGLKSLVTRTPNKQH
                     "
     gene            812..1501
                     /gene="ftsE"
                     /locus_tag="PK818_09780"
     CDS             812..1501
                     /gene="ftsE"
                     /locus_tag="PK818_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HQY83057.1"
                     /translation="MPVLRFDHVSKQYPGGLVALDDVDFSVETGEMLFVTGHSGAGKS
                     TLLKLIQLAERPSRGAVLFAERNLAKVRGGRIAHHRREVGVVYQDHRLLMDHSVAENV
                     ALPLVLRGVRRGEVGKRVRVLLEKLGLGGRERALPSQLSAGEQQRVGIARAVIAEPAM
                     LIADEPTGNLDPTLSAEIMALFAALPERGTSVLIASHDLGLVKRMRKRVLVLDHGRLV
                     DDIAPEDLADE"
     gene            1494..2444
                     /gene="ftsX"
                     /locus_tag="PK818_09785"
     CDS             1494..2444
                     /gene="ftsX"
                     /locus_tag="PK818_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HQY83058.1"
                     /translation="MNERSQGAQASRLGTWLDHHGFSIVASLGRLLRRPWATLLTIGV
                     MALALALPLGLWVVLDNMARLGGEVHASRDIAIFLKPEVGVAKAQAIAGSLRARGDVA
                     TVVLVTPRQALQELRARPELAAAIDAMGADAAQAALPSVLRVSPRGDEQLLAESLRTL
                     PEAERVQHDAVWRERLDAWLRFGGRVVLVLAALFGLGALLVVGNTVRLDIQSRREEIG
                     VLQLLGASDGFVRRPFLYLGAWYGLAAGALALGVLTAAWMALRKPLAELAAEYGSSFA
                     LRGLDPLPAACVLLAAGALGWLGAGLVTGHYLRQTRPVER"
     gene            2452..3297
                     /locus_tag="PK818_09790"
     CDS             2452..3297
                     /locus_tag="PK818_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQY83059.1"
                     /translation="MKQDLQHVVSDAPRVMVVDGSRLVRKLIGDTLLRDLPNAEVIGC
                     GGLEEARAVLANGVVDLVTTALVLPDGDGLALARMVRETAGQAYVPVIVVSGNAQEAL
                     ETRAFTEDITDYFDKGLGHGALAAFIRGYVQPAPIEGARVLYVEDSKVVAVATKRMLA
                     RQAMQVLHVPSVEEALEHLHKYRGRSDGDVGADLVLSDVYLKGALSGRDLLGQIRGAF
                     GYGKRRLPMVMMTGDDNRDNQAALLREGANDLVLKPIEERLLVTKVLFQLRLAKLPEP
                     GAVSR"
     gene            3459..>3698
                     /locus_tag="PK818_09795"
     CDS             3459..>3698
                     /locus_tag="PK818_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQY83060.1"
                     /translation="MSEQTIRLEPSWKARIGDWFARDDMQQLSAFLRQRKAAGASIYP
                     AGTNIFAAFDATPFDQVRVVILGQDPYHGPGQAHGL"
BASE COUNT          516 a         1253 c         1405 g          524 t
ORIGIN      
        1 gcgccgaggg cgacgcgatc agcttcgcct gcgagcgcta cggcatgtcg ctgccggaca
       61 tcgaggcgtt catcgaccag aagatcccgg gcgaacacgt caccccggag atgctggtcg
      121 cgctgccgcg caagccgcgc gaaggggtgg aagcgcagcc ggaagacggc gagagcatcg
      181 gcgagatctt caaggaggtg cgcgaggcgc gtgccgccga ggacgccaag cgcggcggtg
      241 gccgcggcgg cccgggtggc cgcggcgagc gttcgggtcc gcgcagcggc gaacgccgcc
      301 cgggcggcag cgacagccgt ggcccgcgtc cgccgcgcaa gccgcgggtc gagggcgagg
      361 tcgcggcctc gcccgcggtc gctgcagccc ccgcagcagt ggctccggcg gcagcagtcg
      421 tggccgatgg tgagcgcccg caacgcaagc cgcgccggcg tcgcggcggc aagcgcctgg
      481 accagggcac ggtggccacg ccgccgcagg ccggcgccgg gacggcatcg cccgccgtcg
      541 ccccgcctgc cgccgggccg gctgccgctg gcgcgcctgc tgcacaggcc tcgctgctgg
      601 cgcggatcgg ccgtggcctg aagtcgctgg tcacccgcac gccgaacaag cagcactagg
      661 ccgggacacg gcggcaaagt gaagcttcgc ggccggatgt tcgcgcgctt ccggctcggc
      721 tggtcgcggg attcgcgctg accgctcttc ccgcgctggt cgccgccggc gcctcgggcg
      781 atactgcggg tccccctgag cgacagcgcg catgccggtc ctgcgtttcg accacgtcag
      841 caagcaatac ccgggcggcc ttgtcgccct ggacgacgtg gatttctcgg tggaaaccgg
      901 cgagatgctg ttcgtcaccg gccactccgg cgccggcaag agcaccctgc tcaagctgat
      961 ccagctggcc gaacgcccca gccgcggcgc ggtgctgttc gccgaacgca acctggccaa
     1021 ggtgcgcggc ggccgcatcg cccaccaccg ccgcgaggtc ggcgtggtct accaggacca
     1081 ccgcctgctg atggaccaca gcgtcgccga gaacgtggcg ttgccgctgg tgctgcgcgg
     1141 ggtgcggcgc ggcgaggtcg gcaagcgcgt gcgcgtgctg ctggagaagc tcggcctggg
     1201 cgggcgcgag cgcgcgctgc cgtcgcagct gtcggcgggc gagcagcaac gggtcgggat
     1261 cgcccgcgcg gtgatcgccg aaccggcgat gctgatcgcc gacgaaccca ccggcaacct
     1321 cgatcccacc ctgtcggcgg aaatcatggc gctgttcgcg gcgctgccgg agcgcggcac
     1381 cagcgtgctg atcgccagcc acgaccttgg cctggtcaag cgcatgcgca agcgggtgct
     1441 ggtgctcgac cacggccgcc tggtggacga catcgcgccg gaggacctgg ccgatgaatg
     1501 agcgttcgca aggcgcgcag gccagccgcc tgggcacatg gctggaccac cacggcttca
     1561 gcatcgtcgc cagcctcggc cgcctgctgc gcaggccgtg ggcgaccctg ctgacgatcg
     1621 gcgtgatggc gctggcgctg gcgctgccgc tggggctgtg ggtggtgctg gacaacatgg
     1681 cgcggctggg tggcgaggtg cacgcttcgc gcgacatcgc gatcttcctc aagcccgaag
     1741 tcggggtggc gaaggcgcag gcgatcgccg gttcgctgcg cgcgcgcggc gacgtcgcca
     1801 cggtggtgct ggtcaccccg cggcaggcgt tgcaggaatt gcgtgcacgc cccgagctgg
     1861 cggcggcgat cgatgcgatg ggcgccgacg ccgcgcaggc ggcgctgcca agcgtgctgc
     1921 gggtgagccc gcgcggcgac gagcaactgc tggcggagtc gttgcggacc ctgccggaag
     1981 ccgagcgcgt gcagcacgac gcggtctggc gcgaacgcct ggatgcgtgg ctgcgtttcg
     2041 gcgggcgggt ggtgctggtg ctggccgcgt tgttcggcct cggcgcgctg ctggtggtcg
     2101 gcaacacggt gcgcctggac atccagtcgc ggcgcgagga aatcggcgtg ctgcagttgc
     2161 tcggcgccag cgacggcttc gtgcggcggc cattcctgta cctcggtgcc tggtacggcc
     2221 ttgctgccgg cgcgctggcg cttggggtgt tgaccgcggc gtggatggcc ttgcgcaagc
     2281 cgctggccga actcgccgcg gagtacggca gcagtttcgc cctgcgcggg ctggatccgc
     2341 tgccggcggc ctgcgtgctg cttgcggcgg gtgcgctggg ctggctcggc gccggcctgg
     2401 tgaccgggca ctacctgcgc cagacccgac cggtggagcg ctgagatggc aatgaagcag
     2461 gatctgcaac acgtcgtcag cgatgcgccg cgggtgatgg tggtcgatgg cagccggctg
     2521 gtgcgcaagc tgatcggcga caccctgctg cgcgacctgc cgaatgccga ggtgatcggc
     2581 tgcggcggcc tcgaggaggc gcgcgcggtg ctggccaacg gcgtggtgga cctggtgacc
     2641 accgcgttgg tgctgccgga cggcgatggc ctggcgctgg cgcggatggt ccgcgagacc
     2701 gcggggcagg cctacgtgcc ggtgatcgtg gtctccggca atgcgcagga ggcactggag
     2761 acccgcgcct tcaccgagga catcaccgac tatttcgaca agggcctggg ccacggcgcg
     2821 ctggccgcgt tcatccgtgg ctacgtgcag ccggcgccga tcgagggcgc gcgcgtgctg
     2881 tacgtcgagg acagcaaggt ggtggcggtg gcgaccaagc gcatgctggc gcggcaggcg
     2941 atgcaggtgc tgcacgtgcc gagcgtggaa gaggcgctgg aacacctgca caagtaccgc
     3001 ggacgcagcg atggcgatgt cggcgccgac ctcgtgctca gcgatgtcta cctgaagggt
     3061 gcgctcagcg gccgcgacct gctcggccag atccgcggcg cgttcggcta cggcaagcgc
     3121 cgcctgccga tggtgatgat gaccggcgac gacaaccgcg acaaccaggc cgcgctgttg
     3181 cgcgaaggcg ccaacgacct ggtgctgaag ccgatcgagg aacgcctgct ggtgaccaag
     3241 gtgctgttcc agctgcggct ggcgaaactg ccggagccgg gggcggtcag tcgctgacgg
     3301 gtgtgcggga ggccgatcaa gtggggcccg gcctgtcgcc ataggcgaca ggcgttcggt
     3361 cagccgcaga ccagtggtct gcaaacgggc tgccctcacc cccgatactc gcttggttga
     3421 tcggcctccc acacacccgt cccggaaatc atcgcttcat gtcagagcaa acgatccgcc
     3481 tcgaacctag ctggaaagcc cgcattggcg actggttcgc gcgcgatgac atgcagcagc
     3541 tgtccgcctt cctgcgccag cgcaaggcag ccggcgcgtc gatctacccg gccggcacca
     3601 acatcttcgc cgcgttcgat gccaccccgt tcgaccaggt gagggtggtc atcctcggcc
     3661 aggatccgta ccacgggccg gggcaggcgc acggcctg
//
LOCUS       DAORUK010000183         3651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_33621_length_3651_cov_18.628476, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000183 DAORUK010000000
VERSION     DAORUK010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33621_length_3651_cov_18.628476"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..2489)
                     /locus_tag="PK818_09800"
     CDS             complement(132..2489)
                     /locus_tag="PK818_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQY83061.1"
                     /translation="MKTKHLALALAAALACPTLVHAQQAEGARATATDDTPESDARRV
                     ETPDPQLPTVTVVGVRVIPLPRADAVRADAALAAGAAASSDTAQLLRGIPGLSLQAGG
                     GVSSLPSIRGLADDRVRIVVDGMDLVSSCGNHMNPPLSYIDPTRASKLEVFAGITPVS
                     LGGDSLGGTIRVESAEPVFAGAGEGHLVAGKLGAFHRSNGDARGMDVATTFAGEHLSL
                     DYTGSIAEAGNYRAGGDFKPAGPAAAGRGWLDADEVGSTYYRSQNQSLRLALRHGQHL
                     VQLRYGRQDIPRQGFANQRMDMLRNDSEQWALQYQGGFGWGGLEASAFHEHTRHGMQF
                     GPDKLYWYAPTAAQDGQPGPVCMMGMACYAAGMPMETEGTTRGASLKADIRLSARDAL
                     RLGAEVQLYRLDDWWPASGRMMGPDAFVNINDGRRDRHAGYLEWEAEWNPRWLTQAGL
                     RLERVDTDAGPVQSYNSKFSPADVVAFNAAQRQRQDDNLDATLLARYTPSPTLRLEFG
                     LARKTRSPNLYERYAWSTGGMAMLMVNMAGDGNGYVGDLGLKPETARSLSVNADWHDA
                     ADSRWNLRVTPWITRVDDYIDAARCFTPVPPGQTMPMPTPCNADNLVRTQGFVYLRFA
                     NQSAQLHGVDLSARFPVVEGARLGRVHGEARLGYVQGRNRSTDDNLYNMMPLHADFAL
                     EQASGGWNGRIELELARAKQDVSATRNEVATAGYGLLHLRGSYQWRALRLEFGAENLF
                     DRFYQLPLGGAYLGQGKTMSGTGVPWGLAMPGKGRNLYASVTLAF"
     gene            complement(2555..2905)
                     /locus_tag="PK818_09805"
     CDS             complement(2555..2905)
                     /locus_tag="PK818_09805"
                     /inference="COORDINATES: protein motif:HMM:NF022610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HQY83062.1"
                     /translation="MRGLALVAAALLALAPSVSRLLPDAMAAPAGTEAMCTTEGLQAR
                     VPVFGEDAPALPRNHQECGYCALLASALPLPPALAMDVPTVAAGAATLLRRLAPFVAR
                     SNFPALGSRGPPAL"
     gene            3068..3547
                     /locus_tag="PK818_09810"
     CDS             3068..3547
                     /locus_tag="PK818_09810"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HQY83063.1"
                     /translation="MARNGAVAVVALLLAACTSLAPGVVRQGAIRIDHMVLSASGELG
                     TAVDAYAGFDNRGGSDRLLGIECDCAASIELHTVVRAGGTVSMTNTFPLELPAGARTE
                     VKPPGIPLHFMLIDTRRRFAAGERVPMRLRFERAGTVAVDFTVVEKSADGWAAWPGH"
BASE COUNT          516 a         1268 c         1341 g          526 t
ORIGIN      
        1 gccaaggcgc tgcgcaagct gcgccacccg tcgcgttcgg aaaccctgcg cagcttcctc
       61 gacatcgagt aagccgggcc gcccgggttc gtcgagaagg ccccgcacgc ggggcctttt
      121 cgttgccgcg ctcagaacgc cagggtcacc gaggcgtaca ggtttcggcc cttgccgggc
      181 atcgccagcc cccacggcac cccggtcccg gacatggtct tgccctggcc gaggtaagcc
      241 ccgcccagcg gcagctggta gaagcggtcg aacaggtttt ccgcgccgaa ctccagccgc
      301 agcgcccgcc actggtagct gccgcgcagg tgcagcaggc cgtagccggc ggtggcgacc
      361 tcgttgcggg tcgccgagac gtcctgcttg gcccgcgcca gctccagctc gatgcgcccg
      421 ttccagccgc cgctggcctg ctccagcgcg aagtcggcat gcagcggcat catgttgtag
      481 aggttgtcgt cggtgctgcg gttgcggccc tgcacgtagc ccaggcgcgc ctcgccgtgg
      541 acccggccca ggcgcgcgcc ctccaccacc gggaaccggg ccgacaggtc gaccccgtgc
      601 agctgcgccg actggttggc gaagcgcagg tagacgaagc cctgggtccg caccaggttg
      661 tcggcgttgc acggcgtcgg catcggcatg gtctggcccg gcggcaccgg ggtgaagcag
      721 cgcgcggcgt cgatgtagtc atcgacccgg gtgatccacg gcgtcacccg caggttccag
      781 cggctgtcgg cggcatcgtg ccagtcggcg ttgacgctca ggctgcgcgc ggtttccggc
      841 ttcagcccga ggtcgcccac gtagccgttg ccgtcgccgg ccatgttgac catcagcatc
      901 gccatgccgc cggtggacca ggcgtaacgc tcgtacaggt tgggcgaacg ggtcttccgg
      961 gcaagcccga attccagccg cagggtcggc gacggggtat agcgcgcgag cagggtggcg
     1021 tcgaggttgt cgtcctggcg ttggcgctgg gcggcattga aggcaaccac gtcggccggg
     1081 ctgaacttgc tgttgtagga ctgcaccggg ccggcatcgg tgtccacgcg ctccaggcgc
     1141 aggccggcct gggtcagcca gcgcgggttc cactcggcct cccactccag gtagccggcg
     1201 tggcggtcgc ggcggccatc gttgatgttc acgaacgcat ccggccccat catgcggccg
     1261 gaggcaggcc accagtcatc gagccggtac agctgcactt cggcgccgag ccgcagcgca
     1321 tcgcgcgccg acaggcggat gtcggccttc aggctggcac cgcgggtggt gccctcggtc
     1381 tccatcggca tgccggcggc gtagcaggcc atgcccatca tgcagaccgg gcccggctgg
     1441 ccatcctggg ccgcggtcgg ggcgtaccag tagagcttgt cggggccgaa ctgcatgccg
     1501 tggcgggtgt gctcgtggaa ggcggaggct tccagcccgc cccagccgaa tccgccctgg
     1561 tactgcagtg cccactgctc gctgtcgttg cgcagcatgt ccatgcgctg gttggcgaaa
     1621 ccctggcgcg ggatgtcctg gcggccgtag cgcagctgga ccaggtgctg gccatggcgc
     1681 agggccagcc gcagcgactg gttctgcgac cggtagtagg tcgagccgac ctcgtcggca
     1741 tccagccagc cacggccggc cgcggccggg ccggcgggct tgaagtcgcc gccggcgcgg
     1801 tagttgccgg cttcggcgat ggagccggtg tagtccaggc tgaggtgttc accggcgaag
     1861 gtggtggcca cgtccatgcc gcgggcatcg ccattgctgc gatggaaggc gccgagcttg
     1921 ccggcgacga ggtggccctc gccggcgccg gcgaacaccg gctcggcgga ctcgacgcgg
     1981 atggtgccgc cgaggctgtc gccgcccagg ctgaccgggg tgatgccggc gaagacctcg
     2041 agcttcgagg cccgggtcgg gtcgatgtag gacagcggcg ggttcatgtg gttgccgcag
     2101 gaggagacca ggtccatgcc gtcgaccacg atccgcacgc ggtcatcggc aaggccacgg
     2161 atcgagggca ggctggagac cccgccgccg gcctgcaggg acagcccggg aatgccccgc
     2221 agcaactgcg cggtgtcgga actggcggcg gcgccggcgg ccagcgcggc gtcggcccgc
     2281 accgcgtccg cacgcggcag cggaatcacg cggacgccga ccacggtgac ggtcggcaat
     2341 tgcggatccg gggtttcgac gcgcctggcg tcgctttcgg gggtgtcgtc ggtggcggtg
     2401 gcgcgtgcgc cttccgcctg ctgggcgtgg acaagggtcg ggcaggcgag cgcggccgcc
     2461 aacgccaggg cgaggtgctt ggttttcatg gaatgctctt cggaagggaa cggttcgggc
     2521 gcgggcaatg ccctgcgccg ccacgcaacg ccatctaaag ggcaggagga ccccgcgagc
     2581 cgagtgcggg gaagttggat ctcgccacga acggcgccag ccgccgcagc agcgtggcgg
     2641 cgccggcggc cacggtgggc acgtccatcg ccagcgccgg tggcagcggc agcgcggacg
     2701 ccagcagcgc gcagtaaccg cattcctggt ggttgcgcgg cagcgccggg gcgtcttcgc
     2761 cgaaaaccgg cacgcgggcc tgcaggccct ccgtggtgca catcgcctcg gtgccggcgg
     2821 gggctgccat cgcgtccggc agcagccggc tcacgctcgg cgcaagcgcc agcagcgcgg
     2881 cggccaccag ggccagtccg cgcatgaatt ccaagggacg cgccaggatc ctcaccccgt
     2941 cactgtaggc actccgccgc cgccggcgtt gatgcacgtc aagcgtgccg ccgttgccgc
     3001 cgccggcccc atggcccatg gcccgcgtac actgcgccga aacacagcca ggggcacgcg
     3061 catggacatg gccaggaacg gagcggtggc ggtggtggca ttgctgctgg cggcgtgcac
     3121 cagcctggcg ccgggcgtgg tgcgccaggg cgcgatccgg atcgaccaca tggtgctgtc
     3181 ggcgagcggc gagctgggca ccgcggtcga cgcctacgcc ggcttcgaca accgcggcgg
     3241 cagcgaccgc ctgctcggca tcgaatgcga ctgcgcggcc tcgatcgaat tgcacacggt
     3301 ggtgcgcgcg ggcggcacgg tcagcatgac caacactttc ccgctggagc tgccggccgg
     3361 cgcacgcacc gaggtcaagc cgcccggcat cccgctgcac ttcatgctga tcgacaccag
     3421 gcgccgcttc gccgccggcg agcgggtgcc gatgcgcctg cgattcgaac gcgccggcac
     3481 ggtcgcggtg gacttcaccg tcgtcgagaa aagcgccgac ggctgggccg cctggccagg
     3541 ccactgatcc cgcgcgcacc cggccacgcc ggggtgcccg ctacaattcc cgcgcgacgg
     3601 gcctatagct caatggttag agcagaagac tcataatctt ttggttccag g
//
LOCUS       DAORUK010000184         3625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_34026_length_3625_cov_17.570868, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000184 DAORUK010000000
VERSION     DAORUK010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34026_length_3625_cov_17.570868"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PK818_09815"
     CDS             <1..667
                     /locus_tag="PK818_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HQY83064.1"
                     /translation="ISGANLARNWAMIPHVTQHDDADITDLEALRVALNDENARAIAA
                     GKAGKLTMLAFLIKASVAALKKYPNFNASLDGDNLVLKQYYNIGFAADTPNGLVVPVL
                     RDADQKGVMEIAREMGELAAAARDGKLAPAQMQGGCFTISSLGGIGGTAFTPIINAPE
                     VAILGVSKSDMKPVWNGQEFRPRLMLPLSLSYDHRVIDGAAAARFTAYLAQLLADLRR
                     AML"
     gene            679..2505
                     /gene="lpdA"
                     /locus_tag="PK818_09820"
     CDS             679..2505
                     /gene="lpdA"
                     /locus_tag="PK818_09820"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017114723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQY83065.1"
                     /translation="MADIESKVPDIGGHGDVPVIEILVAVGDTVKKDQGLVTLESDKA
                     TMEVPAIADGVVKALKVKLGDVVSEGALIAVIAASDSGTAVQSGAAASPPAPVATATR
                     AEAAAPGPAPAASPAPATPAPQAAASSGRTADIDCALVVIGAGPGGYTAAFRAADLGL
                     DTVLIERYPSLGGVCLNVGCIPSKALLHAADVIDQAAHASDFGVDFGKPKINIDTLRG
                     YKEKVVGQLTKGLAGMARQRKVRVVQGTAKFVSANELEISVEAGVGADGKAQLLHFAN
                     CIIAAGSQPVKLPSFPWDDARVMDSTDALDLADVPKKLLVVGGGIIGLEMATVYRALG
                     SEVTVVEFMPQLMPGADLDLVKPLADRLKKHGIAIHLKTKVVEAKAQKNGIACTFEGE
                     SIPDGKLYDRVLVSVGRAPNGGKLDAGKAGVEVTERGFIDVDRQMRTNVPHIFAIGDL
                     VGQPMLAHKATHEGKLAAEVAAGEKKEWVARVIPSVAYTDPEIAWVGVTETEAKAKGL
                     KVGVGKFPWAASGRAIGLSRTEGFTKLIFDEDSHRIIGGGIVGVHAGDLIAEIGLAIE
                     MGCEVADIGHTIHPHPTLSESVGMAAEVFDGSITDLYIPRKR"
     gene            complement(2540..2743)
                     /locus_tag="PK818_09825"
     CDS             complement(2540..2743)
                     /locus_tag="PK818_09825"
                     /inference="COORDINATES: protein motif:HMM:NF021362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HQY83066.1"
                     /translation="MAKTLSFASLHLGVAFSVGWLLTGKWWMGGLLAVIEPACNTVVF
                     HFHEKLWKRIEARRALAAGPVTR"
     gene            3053..3451
                     /locus_tag="PK818_09830"
     CDS             3053..3451
                     /locus_tag="PK818_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83067.1"
                     /translation="MLNSAAAGRRLALRVVVIQAITTLLTALACLMIGPRTALAALAG
                     GGAMALGSLAAAWGAFGGGVVGAGMALGRLLLGTAAKWLVVAVGLFLAMAVWRLPAVP
                     VLAGAAMAAAALMVSMKFLAAQRSTTRANA"
BASE COUNT          599 a         1172 c         1300 g          554 t
ORIGIN      
        1 gatttccggc gccaacctcg cgcgcaactg ggcgatgatc ccgcacgtca cccagcacga
       61 cgacgccgac atcaccgacc tcgaggcgct gcgcgtcgcc ctgaacgacg agaacgccag
      121 ggcgatcgcc gcgggcaagg ccggcaagct gaccatgctt gcgttcctga tcaaggccag
      181 cgtggcggcg ctgaagaagt atccgaactt caacgcctcg ctggacggcg acaacctggt
      241 cctcaagcag tactacaaca tcggcttcgc ggcggacacg ccgaacgggc tggtggtgcc
      301 ggtgctgcgc gatgccgacc agaagggcgt gatggagatc gcccgggaaa tgggcgaact
      361 ggccgccgcc gcgcgcgacg gcaagcttgc gcccgcgcag atgcagggcg gctgcttcac
      421 catcagctcg ctgggcggga tcggcggcac cgcgttcacc ccgatcatca acgcaccgga
      481 agtggcgatc ctcggggtgt cgaagtcgga catgaagccg gtctggaacg gccaggaatt
      541 caggccgcgc ttgatgctgc cgctgtcgct gagctacgac caccgggtga tcgacggcgc
      601 cgccgcggcg cgcttcaccg cctacctcgc acaactgctg gccgacctcc gtcgggccat
      661 gctctgacgg aggcggacat ggcggacatc gaaagcaagg ttccggacat cggcggccac
      721 ggcgacgtgc cggtcatcga aatcctggtc gcggtcggcg acacggtgaa gaaggaccag
      781 ggcctggtca ccctggaatc cgacaaggcc accatggaag tgccggcgat cgccgatggc
      841 gtggtcaagg cgctgaaggt caagctgggc gacgtggtgt cggaaggcgc gctgatcgcg
      901 gtgatcgccg cgtctgacag cggaacggct gtccaaagcg gcgcggctgc atctccaccc
      961 gcacctgtcg ctacagcaac cagggccgaa gctgcggcgc ccgggcctgc gcccgccgcg
     1021 agccctgcgc ccgcaacgcc cgcgccgcag gccgcggcat cctccggccg cacggccgac
     1081 atcgactgcg cgctggtggt gatcggcgcc ggccccggcg gctacaccgc cgcgttccgt
     1141 gcagcggacc tcgggctcga tacggtgttg atcgaacgct accccagcct tggcggggtc
     1201 tgcctcaacg tcggctgcat cccgtcgaag gcgctgctgc atgcggccga cgtgatcgac
     1261 caggccgcgc atgccagcga cttcggcgtc gatttcggca agccgaagat caacatcgac
     1321 accctgcgcg gctacaagga aaaggtcgtc ggccagctca ccaagggcct ggccggcatg
     1381 gccaggcagc gcaaggtgcg ggtggtgcag ggcaccgcga aattcgtctc ggcgaacgaa
     1441 ctggaaattt ctgtggaagc gggcgtcggc gcggacggca aggcgcaact gctgcacttc
     1501 gccaactgca tcatcgccgc cggctcgcag ccggtgaagc tgccgtcgtt cccgtgggac
     1561 gacgcgcggg tgatggattc caccgatgcg ctggacctcg cggacgtgcc gaagaaattg
     1621 ctggtcgtcg gcggcggcat catcggcctg gaaatggcga cggtgtaccg cgcgctgggc
     1681 agcgaggtga ccgtggtcga gttcatgccg cagctgatgc cgggcgccga cctcgacctg
     1741 gtcaagccgc tggcggaccg gctgaagaag cacggcattg caatccacct caagaccaag
     1801 gtggtcgagg ccaaggcgca gaagaacggc atcgcctgca cgttcgaggg cgagagcatc
     1861 ccggacggca agctgtatga ccgcgtgctg gtgtcggtgg gccgcgcgcc gaatggcggc
     1921 aagctcgatg cgggcaaggc cggcgtggag gtgaccgaac gcggcttcat cgacgtcgac
     1981 cggcagatgc gcaccaacgt gccgcacatc ttcgcgatcg gcgacctggt cggccagccg
     2041 atgctggcgc acaaggcgac gcacgagggc aagctggcgg cggaagtcgc ggccggcgag
     2101 aagaaggagt gggtggcccg ggtgatcccg agcgtggcct acaccgatcc ggagatcgcc
     2161 tgggtcggcg tcaccgaaac cgaggcgaag gcgaagggcc tgaaggtcgg cgtcggcaag
     2221 ttcccgtggg cggcttcggg ccgcgccatc ggcctgtcgc gcaccgaggg cttcaccaag
     2281 ctcatcttcg acgaggacag ccatcgcatc atcggcggcg gcatcgtcgg cgtgcatgca
     2341 ggcgacctga tcgcggagat cgggctggcg atcgagatgg gctgcgaagt cgccgacatc
     2401 ggccatacca tccacccgca tccgaccctg agcgaatcgg tggggatggc ggccgaggtg
     2461 ttcgacggca gcatcaccga cctgtacatc cccaggaagc gctgagccgc ggcgccgccg
     2521 ctgctgcggc cgcggggcgt cagcgcgtga ccggtccggc ggcgagggcg cgccgggcct
     2581 cgatccgctt ccacagcttt tcgtggaaat ggaagaccac ggtgttgcag gccggttcga
     2641 tcaccgccag caggccgccc atccaccatt tgccggtcag cagccagccc accgagaacg
     2701 ccacgcccag gtgcagggaa gcaaagctca gggtcttggc caccggcctg tccttgaatg
     2761 cgaatgatta tcattctatc ccaatgacct gcagtttcaa ggccacgggc ctgaaaaaga
     2821 agccccggcg cggacggccg gggctcctgg cgcccttcct gagctgacga ggaggggagc
     2881 gggcgcccag cacgtctgac acccgtgcgc gctggaagtt tcccgtggcc cggagtcttt
     2941 cggcggttgc cggcggattt tcgcgcctgc tatacttttg cggcttcgcg gggcggtttt
     3001 gatggcttga tgaagccgac ctgcggcacc gtttcgaagt cgggtcctcc gcgtgctcaa
     3061 ttccgctgcc gcgggccggc ggctggcgct gcgggtggtg gtcatccagg ccatcaccac
     3121 gctgctgaca gccctggcct gcctgatgat cgggccgcgc accgcgcttg cggcgctggc
     3181 cggtggcggt gcgatggcgc tgggaagcct ggcggcggcc tggggcgcgt tcggcggcgg
     3241 cgtggtcggg gccggcatgg ccctgggacg gttgctgctg ggaaccgccg cgaaatggct
     3301 ggtggtcgcc gtgggcttgt tcctggcgat ggcggtatgg cggttgccgg cggtgccggt
     3361 actggccggg gcggcgatgg ccgcggcggc cttgatggtt tcgatgaaat tcttggcggc
     3421 acagcgcagt acgacgaggg cgaacgcgtg atcctggaag gcatggtgct ggaaccgagc
     3481 ggcggcgaat ccggtggggg tggcctgacc ggctacatcg tccaccacct gacccacaac
     3541 gtggtctacg tggccgggca gaagctgcac ttcgactcgt ggatggtcgc gctgctgctg
     3601 ggcctgctgg gctgcttcct gctgt
//
LOCUS       DAORUK010000185         3516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_35716_length_3516_cov_16.975152, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000185 DAORUK010000000
VERSION     DAORUK010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35716_length_3516_cov_16.975152"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PK818_09835"
     CDS             complement(<1..199)
                     /locus_tag="PK818_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HQY83068.1"
                     /translation="MKISADRQDAHRAPLRDHVATSVRRYLRDLDGCATDDLYNIALR
                     ELEIPLFAEVLRHCDGNQSRAA"
     gene            complement(326..832)
                     /locus_tag="PK818_09840"
     CDS             complement(326..832)
                     /locus_tag="PK818_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HQY83069.1"
                     /translation="MSHAPSFMRAPAPVRSRRGEWIALVLLALALLAQLFFSQRAQLA
                     ADARWRPLVLSACGALRCSVPAWREPGAFSMLSRDVIAVPGRPGVLRVQASFRNDAAW
                     PQPWPALVLALSDAEGRQLGVRRFQPRDYLGAAPGRALLAPGQATQLAFDILEPGPGV
                     VAFDFRFE"
     gene            complement(829..1740)
                     /gene="prmA"
                     /locus_tag="PK818_09845"
     CDS             complement(829..1740)
                     /gene="prmA"
                     /locus_tag="PK818_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019338963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HQY83070.1"
                     /translation="MPYLQLSLPCTEAQQPRVEHALEDMGALAVTLQDAHLDAADEQA
                     IFEPGVGEIPLWNEMTLTALFEEHTDALVLLAALEAFDPGLDWSQARFEKVEDQDWER
                     AWLDQFQPMRFGERTWIVPWDHDLPDEANVADAAIVRLDPGLAFGSGTHPTTALCLQW
                     LDGLASRWELQGREVLDFGCGSGILALAAMKLGAARAVGVDNDPQALIATADNAQRNG
                     EAIDVHMPQDEPVRSYPVVVANILATALDTLAETLAARVMKGGRLAMSGILSGQEDEL
                     LLRYAEWFDDLRVARQEDWVRIEGLRR"
     gene            complement(1814..3046)
                     /locus_tag="PK818_09850"
     CDS             complement(1814..3046)
                     /locus_tag="PK818_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83071.1"
                     /translation="MQRRHLLCLALVTALTALPAASQKGKGPKTLVYIDVATHNFAGM
                     PDFGGLGGFMAKRMGGDTGPKGYPESRNIPAGTGKFLDIALYNGLKPGVEAQQLVPAG
                     LAVGKSLPLLPPVTEPGGSERYDGKIPDVEVKIYQYWGCGAAVRPGQPKVFSFKMKKG
                     QMETRGSMAPGLFVPDRDIDATPSYAVWPNKKNTKRVSDRSSMVGAHQITGDGVPASL
                     QFELTQNADFMPKIALQSRGEPTDAIAVNWQPVDRARAYFLTGTGIKSEHEFVMWSSA
                     EVAGASSELLNYLTGSYIDKWLKQKVLLPSTSTSCTVPKGIFAGSGGGGGGEMGGMGM
                     LTMIAYGPETNIVYPPKPADPKQPWNPEWNVRVRTKSTAFAMLGLDMGDMQEDDGEGQ
                     QQQKKPSMKGLLKGILGG"
     gene            complement(3247..>3516)
                     /locus_tag="PK818_09855"
     CDS             complement(3247..>3516)
                     /locus_tag="PK818_09855"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017911501.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HQY83072.1"
                     /translation="VDSHIYEGYRVPPNYDSMIGKLIVHGATREQAIARMRVALSEMV
                     VDGIKTNVPLQQRILADGGFAQGGQNIHYLEKKLAERKEKALSVI"
BASE COUNT          499 a         1208 c         1208 g          601 t
ORIGIN      
        1 tcgcggcgcg gctctggttg ccgtcgcagt ggcgcagcac ttcggcgaac agcgggattt
       61 ccagctcgcg cagcgcgatg ttgtacaggt cgtcggtggc gcagccgtcg aggtcgcgca
      121 ggtagcggcg cacggaggtc gccacgtggt cgcgcaatgg cgcgcgatgt gcgtcctggc
      181 ggtcggcgga aatcttcaag cggaaccctc gatgcgacca cggcgagcga atccatcgcc
      241 gcgggagggc gagtctagcg cgcgcgccgc gccgctgtca ttgcggctgc gcattcatcg
      301 aaaaagaaaa atcgcgcgcg cgcggtcact cgaagcggaa gtcgaacgcg accacgcccg
      361 ggcccggttc gaggatgtcg aacgccagtt gcgtggcctg gccaggtgcg agcagggcgc
      421 gcccgggtgc ggcaccgagg tagtcgcgcg gctggaagcg gcggacgccg agctgccggc
      481 cttcggcatc cgacaacgcc agcaccagcg ccggccatgg ctgcggccac gccgcgtcgt
      541 tgcggaagct ggcctgcacc cgcagcacgc cggggcggcc gggcaccgcg atcacgtcgc
      601 gcgagagcat gctgaaggcg cccggttcgc gccatgccgg cacgctgcag cgcagcgccc
      661 cgcatgccga caggaccagc ggccgccagc gggcatccgc ggcgagttgc gcgcgctggc
      721 tgaagaacag ttgcgccagc agcgccagcg ccagcagcac cagtgcgatc cattcgccgc
      781 gccgcgaacg cacgggcgcc ggcgcgcgca tgaagctggg tgcgtggctc accggcgcag
      841 gccctcgatc cgcacccagt cctcttgccg ggcgacgcgc aggtcgtcga accattcggc
      901 atagcgcagc agcagttcgt cttcctggcc ggacaggatc ccggacatcg ccagccgccc
      961 acccttcatc acccgcgccg ccagtgtctc cgccagggtg tccagcgcgg tggcgaggat
     1021 attggcaacc accaccgggt aactgcgcac cggttcgtcc tgcggcatgt ggacgtcgat
     1081 cgcctcgccg ttgcgttgcg cgttgtcggc ggtggcgatc agcgcctgcg gatcgttgtc
     1141 gacgccgacc gcgcgcgcgg caccgagctt catcgcggcc agcgcgagga tcccgctgcc
     1201 gcagccgaag tcgagcacct cgcgcccctg cagttcccag cggctggcca gtccatccag
     1261 ccattgcagg cacagcgcgg tggtcggatg ggtgccggaa ccgaacgcca ggccgggatc
     1321 gaggcgcacg atcgcggcat ccgcgacgtt ggcttcgtcc ggcaggtcat ggtcccacgg
     1381 cacgatccag gtgcgttcgc cgaagcgcat cggctggaac tggtccagcc acgcgcgttc
     1441 ccagtcctgg tcctcgactt tctcgaagcg cgcctggctc cagtccagtc caggatcgaa
     1501 cgcttccagt gcggccagca ggaccagcgc gtcggtgtgt tcctcgaaca gcgcggtcag
     1561 ggtcatttcg ttccacagcg ggatttcgcc gacgccgggt tcgaagatcg cctgttcgtc
     1621 ggccgcgtcc aggtgcgcgt cctgcagggt taccgccagc gcgcccatgt cctccagcgc
     1681 gtgttcgacg cgcggctgct gggcttcggt gcagggcagg ctgagttgca ggtagggcat
     1741 gcgggggaat ggcgcgggag accacgccat tctcgcagtt tcgccgctgc ggtggatcgg
     1801 gcggcggttt cgctcagccg ccgaggatgc ccttcagcag gcccttcatc gagggcttct
     1861 tctgctgctg ctggccttcg ccgtcgtctt cctgcatgtc gcccatgtcc aggcccagca
     1921 tcgcgaacgc ggtggacttg gtgcgcacgc gcacgttcca ttccgggttc cacggctgct
     1981 tcgggtcggc cggcttcggc gggtacacga tgttggtttc cgggccgtag gcgatcatgg
     2041 tcaacatccc catcccgccc atctcgccgc cgccgccacc accggagccg gcgaagatgc
     2101 ccttgggcac cgtgcagctg gtgctggtgc tgggcagcag caccttctgc ttcagccact
     2161 tgtcgatgta ggagccggtc aggtagttca gcagttcgct gctggcgccg gcgacctcgg
     2221 cgctgctcca catcacgaat tcatgctcgc tcttgatccc ggtgccggtc aggaaatagg
     2281 cgcgggcgcg gtcgaccggc tgccagttca ccgcgatcgc gtcggtcggt tcgccgcggc
     2341 tttgcagggc gatcttcggc atgaagtccg cgttctgggt cagctcgaac tgcagcgatg
     2401 ccggcacgcc atcgccggtg atctggtggg cgccgaccat cgaggagcgg tccgacaccc
     2461 gcttggtgtt cttcttgttc ggccacacgg cgtaggacgg ggtggcatcg atgtcgcggt
     2521 cgggcacgaa caggccaggc gccatgctgc cgcgggtctc catctggccc ttcttcatct
     2581 tgaagctgaa gaccttcggc tggcccgggc gcaccgccgc gccacagccc cagtactggt
     2641 agatcttcac ttcgacatcg gggatcttgc cgtcgtagcg ctcgctgcca ccgggctcgg
     2701 tgaccggcgg cagcagcggc agcgacttgc cgaccgcaag cccggccggc accagttgct
     2761 gcgcttccac ccccggcttg aggccgttgt acagggcgat gtcgaggaac ttgccggtcc
     2821 cggcggggat gttgcgggat tccggatagc ccttcggccc ggtgtcgccg cccatgcgct
     2881 tggccatgaa gccgccaagg ccgccgaaat ccggcatccc ggcgaagttg tgggtggcca
     2941 cgtcgatgta caccagggtc ttcgggccct tgcccttctg gctggccgcg ggcagggcgg
     3001 tcagcgcggt gacgagggcg aggcagagca ggtggcggcg ttgcatggga gctccggatt
     3061 ggtgccgagt gttccactgt aaagcggaat ttcggcccga aagcggacat cccggccgag
     3121 ccgcagcggc cggtcgcccc tgcggcgaat gtcccccgac accggcctgg cggccgctgt
     3181 ctcctccttg atttcgccgc aggggcgacc ggccccagcg gctctccgga ggttcccgtc
     3241 gccgcgtcag atcaccgaca acgccttttc cttgcgctcg gccagtttct tctcgaggta
     3301 atggatgttc tgcccaccct gggcgaagcc gccgtcggcg aggatgcgct gctgcagcgg
     3361 cacgttggtc ttgatgccgt ccaccaccat ttcgctgagc gccacccgca tccgcgcgat
     3421 cgcctgctcg cgggtggcgc cgtggacgat cagcttgccg atcatcgagt cgtagttcgg
     3481 cggcacccgg tagccttcgt agatgtgcga atccac
//
LOCUS       DAORUK010000186         3471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_36476_length_3471_cov_17.869145, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000186 DAORUK010000000
VERSION     DAORUK010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36476_length_3471_cov_17.869145"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PK818_09860"
     CDS             complement(<1..349)
                     /locus_tag="PK818_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HQY83073.1"
                     /translation="MTESFAELFEASQANWAKIKPGAIVKGIVVAVTNDVVVINAGLK
                     SEGIVPIEQFRNDAGEIDVGIGDEVKVALDSLENGFGETVLSREKAKRAMVWDELEEA
                     LEANATIVGRISGK"
     gene            complement(527..1207)
                     /gene="cmk"
                     /locus_tag="PK818_09865"
     CDS             complement(527..1207)
                     /gene="cmk"
                     /locus_tag="PK818_09865"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HQY83074.1"
                     /translation="MDSAAPVLTIDGPSGSGKGTISRIVAQRLGWHYLDSGALYRAVG
                     VAAGWADLDLDDGGALVRCTFDTVVAFREDGDGELRVLINGNDATDELRTETAGAAAS
                     AIAAIPEVRAALKDRQRAFRQLPGLVADGRDMGTVIFPDAARKVFLTASAEERAARRY
                     KQLKDKGVSVTLDGLLREILARDARDASRAVAPLRPAEDAIRIDTTGLPIEDVVARVL
                     DVVSRSAG"
     gene            1357..1482
                     /gene="ykgO"
                     /locus_tag="PK818_09870"
     CDS             1357..1482
                     /gene="ykgO"
                     /locus_tag="PK818_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HQY83075.1"
                     /translation="MKVLSSLKSAKTRHRDCKVVRRRGKVFVICKSNPRFKARQR"
     gene            1621..2649
                     /locus_tag="PK818_09875"
     CDS             1621..2649
                     /locus_tag="PK818_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010372979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HQY83076.1"
                     /translation="MHESPRFPAEWEPQSAILLAWPHAGTDWADRLGDVEDTYIALVQ
                     AITRFQPALVCVADDDVETYADIRLRSNRVNMARVRFVTVAYDDTWLRDSGPITLRAG
                     NGFNLLDFRFTAWGGKFEAGLDDLLVETLAAQGVFGDAARQRIDFALEGGGIETDGHG
                     TLLTTWRCLHERHPDASREELTAKLSAWLHQDRVLWLDHGALEGDDTDAHIDTLARFA
                     PDDAIVFQACDDEADSHYADLKAMAEEIAALRTRDGNPYRLFPLPWARPVLDEGRRLA
                     ASYANFLIVNGAVLMPAYGDPADDAAAAVLAQAFPGREIVQVPCRPLIWQNGSLHCIT
                     MQLPEGLV"
     gene            2649..>3471
                     /locus_tag="PK818_09880"
     CDS             2649..>3471
                     /locus_tag="PK818_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HQY83077.1"
                     /translation="MSRYNKLPVALVQERNHGDAEANLAVIETRVAEAAKSGAKLVLL
                     QELHNGAYFCQHESVDEFDLAETIPGPSTTRLAALAKQHGVVLVSSLFEKRAAGLYHN
                     TAVVYEADGSIAGRYRKMHIPDDPGFYEKFYFTPGDLGFTPIDTSVGRLGVLVCWDQW
                     YPEASRLMALAGAELLLYPTAIGWDPEDAQDEKTRQRDAWVLSHRGHAVANGLPVLSC
                     NRVGHETSPLGASGIDFWGNSHVLGPQGEFIAEAGTSPTLLLAEVDMQRSEHVRRI"
BASE COUNT          552 a         1194 c         1154 g          571 t
ORIGIN      
        1 ccttgccgct gatgcggccg acgatggtgg cgttggcttc cagcgcctct tccagctcgt
       61 cccacaccat ggcgcgcttg gccttctcgc gcgacagcac ggtttcgcca aaaccattct
      121 cgagcgagtc aagggcaacc ttgacctcgt cgccgatgcc gacgtcgatt tcaccggcgt
      181 cgttgcggaa ctgttcgatc ggcacgatgc cttcggactt caggccggcg ttgatcacca
      241 ccacgtcgtt ggtgacggcg accacgatgc ccttgacgat ggcaccgggc ttgatcttgg
      301 cccaattggc ctggctggct tcgaacagtt cggcgaagga ttcggtcatt acggaattca
      361 ttgaggatct ctggtggata cacaggccgg ccgcaggatg cggtggccca ccttgggtct
      421 ggcaccggcg caggacgcgc gtggccatgt gtgggtggat ggaggaaaac cgcgggcagc
      481 cacggggcgc ccacggaacg gttctgcgac gggtgaaacg cgccggctag ccggcggaac
      541 gggacacgac gtccagcacc cgtgcgacca cgtcctcgat gggcaggccg gtggtgtcga
      601 tgcggatggc gtcttcggcc ggccgcaacg gcgccaccgc gcgactggca tcgcgggcgt
      661 cgcgggcgag aatctcccgt agcagaccgt caagtgtgac cgaaacccct ttgtctttca
      721 actgcttata gcgccttgcg gcacgctcct cggcgcttgc ggtcaggaac accttgcgcg
      781 cggcatccgg gaagatcacg gtgcccatgt cgcggccgtc ggcgaccagc cccggcaact
      841 gccggaacgc gcgctggcgg tccttcagcg ccgcccgcac ctcggggatc gcggcgatcg
      901 ccgaggcggc cgcgccggcg gtctcggtgc gcagttcgtc ggtggcatcg ttgccgttga
      961 tcagcacccg caactcgcca tcgccgtcct cgcggaaggc gaccacggtg tcgaaggtgc
     1021 agcggaccag cgcgccgccg tcatcgaggt cgaggtcggc ccagccggcg gccacgccca
     1081 ccgcccggta cagcgcgccg gaatccaggt agtgccagcc aaggcgctgg gcgacgatgc
     1141 ggctgatggt gcccttgccg gaaccggagg ggccatcgat ggtgagtacg ggtgcggcgc
     1201 tgtccatggc gccagattat ggccgggttc gccgcaactg ccgcggcccg cgacatattt
     1261 ggttcaagct gttgattcgg cggaacaaag cccgctacaa tgcgcggctt cgcttcaccc
     1321 gcacccgttc ccaacccact tttccgaggt tctgtcatga aggttctgtc ctccctgaag
     1381 tcggcgaaga cccgtcaccg cgactgcaag gtggttcgcc gtcgtggcaa ggtcttcgtg
     1441 atctgcaagt ccaacccgcg cttcaaggcg cgccagcgct gatcccggat ccgggccgtg
     1501 ctgccgaagg ccgccgcgag gcggcctttt gcgtatctgg cgataccgtg ccgccacgtc
     1561 gctgcacgtt cgcgcatccg gcatagaatc catcgccgtc gtcccgcccc cgagtccagc
     1621 atgcacgagt ccccgcgctt tcccgccgaa tgggaacccc agtcggcgat cctgctggcc
     1681 tggccgcatg ccggcaccga ctgggccgac cgcctgggcg atgtcgagga cacctacatc
     1741 gcgctggtgc aggcgatcac ccgtttccag ccggcgctgg tctgcgtggc cgacgacgac
     1801 gtcgaaacct atgccgacat ccgcctgcgc tccaaccggg tcaacatggc gcgggtgcgc
     1861 ttcgtgaccg tggcctacga cgacacctgg ctgcgcgatt ccggccccat cacgctgcgt
     1921 gccggcaacg gcttcaacct gctggacttc cgcttcaccg cctggggcgg caagttcgaa
     1981 gccggcctcg acgacctgct ggtggaaacc ctggccgcgc agggcgtgtt cggcgatgcc
     2041 gcgcgccagc gcatcgactt cgcgctggaa ggcggcggca tcgagaccga cggccacggc
     2101 accctgctca ccacctggcg ctgcctgcac gaacggcacc cggatgccag ccgcgaggaa
     2161 ctgaccgcga agctgtcggc gtggctgcac caggaccggg tgctgtggct ggaccacggc
     2221 gcactggagg gcgacgacac cgacgcccac atcgataccc tggcccgctt cgcgccggac
     2281 gacgcgatcg tgttccaggc ctgcgatgac gaagcggatt cgcattacgc cgacctcaag
     2341 gcgatggccg aggaaatcgc cgcgctgcgc acccgcgacg gcaatcccta ccgtctgttc
     2401 ccgctgccgt gggcccggcc cgtcctggac gaagggcgcc ggctggccgc cagctatgcc
     2461 aacttcctga tcgtcaacgg tgcggtgttg atgccggcgt acggcgaccc cgccgacgat
     2521 gccgccgccg cggtgctggc gcaggcgttc cccggccgcg agatcgtgca ggtgccctgc
     2581 cggccgttga tctggcagaa cggcagcctg cactgcatca ccatgcaact gccggagggt
     2641 ctggtctgat gtccaggtac aacaagctgc ccgtcgcact ggtgcaggag cgcaaccacg
     2701 gcgatgccga ggccaacctt gcggtcatcg agacccgcgt cgccgaagcc gcgaaatcgg
     2761 gcgcaaaact ggtcctgctg caggaactgc acaacggcgc ctatttctgc cagcacgagt
     2821 cggtggacga attcgacctg gccgaaacca ttcccggccc ttcgactacg cgcctggcgg
     2881 cattggcgaa gcagcatggc gtggtgctgg tcagctcgct gttcgagaag cgcgctgccg
     2941 ggctttacca caacaccgcg gtggtctacg aagccgacgg ctcgatcgcc ggcaggtacc
     3001 gcaagatgca tatcccggac gatccgggtt tctacgagaa gttctacttc acgccgggcg
     3061 acctgggctt cacccccatc gacacgtcgg tcggccggct gggcgtgctg gtgtgctggg
     3121 accagtggta tcccgaggcc tcgcgcctga tggcgctggc cggtgccgaa ttgctgctgt
     3181 atcccaccgc gatcggctgg gacccggagg atgcacagga cgagaaaacc cgccagcgcg
     3241 acgcgtgggt gctgagccat cgcgggcacg cggtggccaa cgggctgccg gtgctgtcgt
     3301 gcaaccgcgt gggccatgag acgtcgccgc tgggcgcgtc cggcatcgac ttctggggca
     3361 acagccacgt gctggggccg cagggcgaat tcatcgccga ggccggcacc tcacccaccc
     3421 tgctgctggc cgaggtggac atgcagcgca gcgagcacgt gcgccggatc t
//
LOCUS       DAORUK010000187         3465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_36569_length_3465_cov_23.169208, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000187 DAORUK010000000
VERSION     DAORUK010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36569_length_3465_cov_23.169208"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PK818_09885"
     CDS             complement(<1..1003)
                     /locus_tag="PK818_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQY83078.1"
                     /translation="MSDKPLTDISFDSFDLHPALLAGLESAGFLRCTPIQALTLPLAL
                     HGRDVAGQAQTGTGKTLAFLVAVIDRLLKRPALADRKAEDPRALILAPTRELAIQIHK
                     DAVKLGDQLGLTFALVYGGVDYDKQRELLQRGTDVIIATPGRLIDYVKQHKVVSLHAC
                     EVCVLDEADRMFDLGFIKDIRFLLRRMPERTTRQTLLFSATLSHRVLELAYEHMNEPE
                     KLVVETEHITAARVRQKLYYPADEEKLPLLISLLSRSEGARTMVFVNTKAFVERVARG
                     LERAGYRVGVLSGDVPQKKRESLLKKFQAGQLELLVATDVAARGLHIDGVSHVYNYDL
                     "
     gene            1211..1537
                     /gene="trxA"
                     /locus_tag="PK818_09890"
     CDS             1211..1537
                     /gene="trxA"
                     /locus_tag="PK818_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQY83079.1"
                     /translation="MSNTVLHATDADFADHVLASDQPVLVDFWAPWCGPCKMIAPALD
                     ELAGTYAGRARIVKVDVDQNQATALKYHVRSIPMLLLFKDGQVQATQIGAVGKGQLAQ
                     MIDKAL"
     gene            1797..>3465
                     /gene="rho"
                     /locus_tag="PK818_09895"
     CDS             1797..>3465
                     /gene="rho"
                     /locus_tag="PK818_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HQY83080.1"
                     /translation="MSDPTNDATGEPAEKRVRKTRVAKASTPESAATPAPPSAPAAAA
                     APAAAPAPQPPAAAPAEASRPQNPGNPGNPGNEGRNENRNEPRNENRSEGGGNEGGDG
                     GGNFDRDGQPRQNNNNRRERFKNRRDRQRDRYREGGMPDDGGNGENFVPRPHPQVPEG
                     FPQYSLGDLKRLPAHKLIEIAEQLQISEGVARARKQDIIFAVLKVLTRHGEGVAADGV
                     LEILPDGFGFLRAAEASYLAGPDDTYISPSQIRRFNLRTGDHVSGRIRWPKDGERYFA
                     LNIVDTINGEPMEASKNKALFENLTPLFPRKKFKLERGDGSSEDITGRILDLMAPQGK
                     GQRALIVSPPKAGKTMMMQQIASAITYNHPDVHLIVLLIDERPEEVTEMQRTVRGEVI
                     SSTFDEPAARHVQVAEMVIERAKRLVEHKKDVVILLDSITRLARAYNNVLPSSGKVLT
                     GGVDANALHRPKRFFGAARNVEEGGSLTIIATALVDTGSAMDKVIYEEFKGTGNSEVH
                     LDRRITEKRVYPAINVNGSGTRREDYLIEPDLLQKIWILRKLLHPMDE"
BASE COUNT          617 a         1232 c         1126 g          490 t
ORIGIN      
        1 gcaggtcgta gttgtagacg tgcgacacgc cgtcgatgtg caggccgcgc gcggccacgt
       61 cggtggccac cagcagttcc agctggccgg cctggaattt cttcagcagc gattcgcgct
      121 tcttctgcgg cacgtcgccg ctgagcacgc cgacccggta gccggcgcgt tccaggccac
      181 gggcgacgcg ttccacgaag gccttggtgt tgacgaagac catcgtgcgc gcgccctcgc
      241 tgcgcgacag caggctgatc agcagcggca gcttctcttc atcggccggg taatagagct
      301 tctgccggac ccgcgcggca gtgatgtgct cggtttccac gaccagcttc tccggctcgt
      361 tcatgtgctc gtaggcgagc tccagcaccc ggtgcgagag ggtggcggag aacagcaggg
      421 tctggcgggt ggtgcgctcc ggcatccgcc tcagcaggaa gcggatgtcc ttgatgaagc
      481 cgaggtcgaa catgcggtcg gcctcatcca gcacgcacac ctcgcaggcg tgcagcgaca
      541 ccaccttgtg ctgcttgacg tagtcgatca accggcccgg ggtggcgatg atcacgtcgg
      601 tgccgcgctg cagcagttcg cgctgcttgt cgtaatcgac gccgccatag accagcgcga
      661 aggtcaggcc cagctggtcg ccgagcttga ccgcgtcctt gtggatctgg atcgccagtt
      721 cgcgggttgg cgccaggatc agtgcgcgcg ggtcttccgc cttgcggtcg gccagcgccg
      781 gacgcttgag caggcggtcg atcaccgcga ccaggaacgc cagggtcttg ccggtgccgg
      841 tctgcgcctg gccggcgacg tcgcggccgt gcagcgccag cggcagggtc agcgcctgga
      901 tcggggtgca gcgcaggaag ccggcggatt ccaggcctgc aagcagggcc ggatgcaggt
      961 cgaagctgtc gaaggaaatg tcggtaagcg gtttgtcgga cacggaggcg gcctctggcg
     1021 gcatggcacg cgcgttcacg ggaacttcgc gctgcacgtt cgcacttgta tgaatggggg
     1081 gcagccacgc agactgccgg gaggatccgg ggctgttggt tcccggcacg gcaccttgaa
     1141 aaccggggtg cattccccaa gtttaccatc ccggcgccgc cgacccggcg cccgagtctt
     1201 tggagccccc atgagcaaca ccgttctgca cgccaccgac gccgatttcg ccgaccacgt
     1261 gctggcctcc gaccagccgg tgctggtgga tttctgggca ccgtggtgcg gcccgtgcaa
     1321 gatgatcgcg ccggcgctgg acgaactggc cggcacctac gccgggcgcg ccaggatcgt
     1381 gaaggtcgac gtcgaccaga accaggccac cgcgctcaag taccacgtgc gctcgatccc
     1441 gatgctgctg ctgttcaagg acggccaggt ccaggctacc cagatcggcg cggtcggcaa
     1501 gggccagctc gcgcagatga tcgacaaggc gctctgagcg cttgcaaccc gcgcggggtt
     1561 cgccccgcgc ctgcgttccg gccgcattgc tccggcgggc cacgcacggc cggccccacc
     1621 gccacgtcct gcccgcagtt gcgcgcgata ccgcgccagt gctagtttcc cgctcgaccc
     1681 ggcgccgatc caccgccccc cggcgcgccg cacccttctg accgctccaa tcggagcctt
     1741 tcccgacgac ccgcccgcaa cccagcgggc gctcgcaacg caagcgagga tttcccttgt
     1801 ccgacccgac caacgacgcc accggcgaac ccgccgagaa gcgcgtgcgc aagacccgcg
     1861 tggccaaggc cagcaccccc gagtccgccg cgactccagc acctccttca gcacctgccg
     1921 ccgcagccgc acctgcagcc gcgccggcac cgcaaccccc cgctgccgca ccggccgagg
     1981 cgtcgcggcc gcaaaatccc ggcaatcccg gcaatcccgg caacgaaggc cgcaacgaaa
     2041 accgcaacga gccgcgcaac gagaaccgca gcgaaggtgg cggcaacgag ggcggcgatg
     2101 gtggtggcaa cttcgaccgc gatggccagc cgcgccagaa caacaacaac cgccgcgagc
     2161 gcttcaagaa ccgccgcgac cgccagcgcg accgctaccg cgaaggcggc atgccggacg
     2221 acggcggcaa cggcgagaac ttcgtgccgc gcccgcatcc gcaggtgccg gaaggcttcc
     2281 cgcagtactc gctgggcgac ctcaagcgcc tgcccgcgca caagctgatc gagatcgccg
     2341 agcagttgca gatcagcgag ggcgtggccc gcgcgcgcaa gcaggacatc atcttcgcgg
     2401 tgctgaaggt actgacccgc catggcgaag gcgtcgccgc tgatggcgtg ctggaaatcc
     2461 tgccggacgg cttcggcttc ctgcgcgcgg ccgaggcgtc ctacctcgcc ggcccggacg
     2521 acacctacat cagcccgtcg cagatccgcc gcttcaacct gcgcaccggc gaccatgtgt
     2581 ccggccgcat ccgctggccc aaggacggcg agcgctactt cgcgctgaac atcgtcgaca
     2641 ccatcaacgg cgagccgatg gaagcgtcga agaacaaggc gctgttcgag aacctgaccc
     2701 cgctgttccc gcgcaagaag ttcaagctgg aacgcggcga cggttccagc gaggacatca
     2761 ccggccgcat cctcgacctg atggcgccgc agggcaaggg ccagcgcgcc ctgatcgtca
     2821 gcccgccgaa ggccggcaag acgatgatga tgcagcagat cgccagcgcg attacctaca
     2881 accatcccga cgtgcacctg atcgtgctgc tgatcgacga gcgcccggaa gaagtcacgg
     2941 aaatgcagcg caccgtgcgc ggcgaagtga tttcctccac cttcgacgaa cccgccgcgc
     3001 gccatgtgca ggtcgcggaa atggtgatcg agcgcgccaa gcgcctggtc gagcacaaga
     3061 aggacgtggt gatcctgctc gactcgatca cccgcctggc ccgcgcctac aacaacgtgc
     3121 tgcccagctc cggcaaggtg ctcaccggcg gcgtcgacgc caacgcgctg caccggccga
     3181 agcgcttctt cggcgccgcc cgcaacgtcg aggaaggcgg cagcctgacc atcatcgcca
     3241 ccgccctggt cgacaccggc agcgcgatgg acaaggtgat ctacgaggaa ttcaagggca
     3301 ccggcaacag cgaagtgcac ctggaccgcc gcatcaccga gaagcgcgtg tacccggcga
     3361 tcaacgtcaa cggctccggc acccgccgcg aggattacct gatcgagccg gacctgctgc
     3421 agaagatctg gatcctgcgc aagctgctgc acccgatgga cgaga
//
LOCUS       DAORUK010000188         3398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_37733_length_3398_cov_19.145977, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000188 DAORUK010000000
VERSION     DAORUK010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37733_length_3398_cov_19.145977"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1785
                     /locus_tag="PK818_09900"
     CDS             <1..1785
                     /locus_tag="PK818_09900"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HQY83081.1"
                     /translation="IGMVSLDVPVQALSSSLESLRSLGQRTALFAPAGTIAVHPEKGV
                     AFAHTLSGYIQRAGRGDLAPMEAARAQGRALQFTHADARSGTTRFSVLQPVGDSGWSL
                     QLSLGRDAMLADLEQATRMLGLIGALVALLAALAVLRLARRITVPLTELTASAGHFAN
                     GEFDWPVPHDARGDEVGVMARALERARDSIRLQLDQIGRYAAEQQKLQSELDIARNIQ
                     RSMLPRDRDFADGSRRYRLQARLEPAKAVGGDFHGHFLHGDGQLWFVVGDVSDKGVPA
                     ALFMVRALTVLEVATAAGDAPDRVLADAARHLAEGNDACMFATVLCGVLDLASGGLAI
                     ASAGHDAPLLRHADGRIEAIVLESGPALGFEAGDDYQVWHGRLQPGDLLFAWTDGVTE
                     ALDRDDRAFGEERLGAALQVAQDAADACDGLLRAVHGFADGAPQSDDITVFAIDCASA
                     PVAGGWPRHLRVPDQRDRLPGLQQALASTLGEAGIEPRRIHDAQVIVEELVCNLMDHG
                     AAAGVDALVLAVAVDAGRVVLDIRDNGAAYDPLAHPPPDLEADIAQRPIGGLGIHLVR
                     ELSQDVQYRRQDGWNALRVVLDAILPPS"
     gene            1803..2186
                     /locus_tag="PK818_09905"
     CDS             1803..2186
                     /locus_tag="PK818_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQY83082.1"
                     /translation="MAMSVRIHPLHENGSQRVEIGGRLDTHTYGELDDRLTLVLASKV
                     RSLLLDLAPLEYISSAGVRSVFRARKLLAERGGKVVIANAQPQVQKVFDIVKAVPLSE
                     IFRSVEEADAYLDRIQKKILANDED"
     gene            complement(2232..>3398)
                     /locus_tag="PK818_09910"
     CDS             complement(2232..>3398)
                     /locus_tag="PK818_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HQY83083.1"
                     /translation="EFMAAVLSSDMDNTDKVVGFLDEARALGLRVLPPDVNGSTHMFE
                     ATDPGTIRYGLGAVKGVGRGACEAIVAAREPGGRFADLLDFCKRVAATGLNKRTLEAL
                     IHAGAMDALAGNRASLMLQLPEVLRATDQIARNRDAGMVDMFGNATGAPDIHIDLPAC
                     DEWPLAQKLHGERETLGHYLSGHPLDPHREDLKRLVGHDLASLDALWESRPQDAQRGW
                     RPEMQAVVAGQVVGMRKRGDSQAFVQLEDGRGRLECAFFGEQWHEFAPLLTRDRILVV
                     EGGLREDEFSGGFALRARRCWDYAQVCDQQAQRLSIRLDLREPGALQRFEQVLQAHAG
                     TTPVLIEATTAAGVGRLSINGGRGLRMDPALPGLLRSLPGVQAVNVHLGKPWLN"
BASE COUNT          475 a         1178 c         1236 g          509 t
ORIGIN      
        1 atcggcatgg tcagcctgga cgtgccggtg caggcgctgt cgtcgtcgct ggaatcgttg
       61 cgctcgctgg ggcagcggac cgcgctgttc gcacccgccg gcaccatcgc cgtgcatccg
      121 gagaagggcg tcgcgttcgc gcacacgctg tccggctaca tccagcgcgc cgggcgcggc
      181 gacctcgcgc cgatggaggc cgcgcgcgcg cagggccgtg ccctgcagtt cacccacgcc
      241 gatgcgcgca gcgggacgac ccgcttctcg gtgctgcagc cggtcggcga cagcggctgg
      301 agcctgcagc tctcgctcgg tcgcgacgcg atgctggcgg acctggagca ggcgacccgg
      361 atgctgggcc tgatcggcgc actggtcgcg ctgctggcgg cgctcgcggt gttgcggctg
      421 gcacggcgga tcacggtgcc gctgaccgaa ctcaccgcca gcgccggcca cttcgcgaac
      481 ggcgaattcg attggccggt gccgcacgat gcgcgcggcg acgaggtcgg ggtgatggcg
      541 cgtgccctcg aacgcgcgcg cgattcgatc cgcctgcaac tcgaccagat cggccgctat
      601 gccgccgagc aacagaagct gcagagcgaa ctggacatcg cccgcaacat ccagcgctcg
      661 atgctgccgc gcgaccgcga tttcgccgac ggatcgcgcc gttaccggtt gcaggcccgg
      721 ctggagccgg cgaaggcggt cggcggcgac ttccatggcc atttcctgca tggcgacggc
      781 cagctgtggt tcgtggtcgg cgacgtttcc gacaagggcg tgccggcggc gctgttcatg
      841 gtccgcgcat tgaccgtgct ggaggtggcg acggcggcgg gcgatgcgcc ggaccgggtg
      901 ctggcggacg ctgcgcgcca ccttgccgaa ggcaacgatg catgcatgtt cgccaccgtg
      961 ctgtgcggcg tgctcgacct cgccagcggc gggctggcga tcgccagtgc gggacacgat
     1021 gcgccgctgc tgcggcacgc ggacggcagg atcgaggcga tcgtgctgga gtccggcccc
     1081 gcactcggct tcgaggcggg cgatgactac caggtctggc acggccgcct gcaaccgggc
     1141 gacctgctgt tcgcctggac cgacggggtg accgaggcgc tcgaccgcga cgaccgtgcg
     1201 ttcggagagg agcgcctggg tgcggccctg caggtcgcgc aggacgccgc ggacgcctgc
     1261 gatggcctgc tgcgggcagt ccatggcttc gcggatggcg cgccgcagtc ggacgacatc
     1321 accgtgttcg ccatcgattg cgcgtccgcg ccggtggcgg gcgggtggcc gcggcacctg
     1381 cgcgtgccgg accagcgcga ccgcctgccc gggctgcagc aggcgctggc gtcgaccctg
     1441 ggcgaagcgg ggatcgagcc ccggcggatc cacgacgcgc aggtgatcgt cgaggaactg
     1501 gtgtgcaacc tgatggacca tggcgcggcg gcgggggttg atgcgctggt gcttgcggtg
     1561 gcggtggatg ccgggcgggt ggtgctggac atccgcgaca acggcgccgc gtacgatccg
     1621 ctcgcgcacc cgccgccgga cctcgaagcg gacatcgcgc agcgcccgat cggcggcctc
     1681 ggcatccacc tggtccgcga actttcgcag gatgtgcagt accggcggca ggacggctgg
     1741 aacgccttgc gcgtcgtgct cgatgcaata ctccctccct cctgaccacg gggtcgcgcc
     1801 gcatggccat gtccgtccgc atccatccgc tgcatgagaa cggcagccag cgcgtcgaga
     1861 tcggcggccg cctggacact cacacctatg gcgaactcga cgaccgcctg accctggtgc
     1921 tggcctcgaa ggtgcgctcg ctgctgctcg acctcgcgcc gctggaatac atcagttcgg
     1981 ccggcgtgcg ttcggtgttc cgcgcgcgca agctgctggc cgagcgtggc ggcaaggtgg
     2041 tcattgccaa cgcgcagccg caggtgcaga aggtgttcga catcgtcaag gcggtgccat
     2101 tgagcgagat cttccgcagc gtggaggaag ccgatgccta cctggaccgg atccagaaga
     2161 agatcctggc caacgacgag gattgagcgc gcacaggtgc gcggcatgcg cggccactcc
     2221 cgcgcgccgg cctagttcag ccagggcttg ccgaggtgca cgttgaccgc ctgcacgccg
     2281 ggcaggctgc gcagcaggcc aggcagcgcc gggtccatgc gcaggccgcg gccgccgttg
     2341 atggaaagcc tgcccacgcc cgcagcggtg gtggcctcga tcagcaccgg ggtggtgccg
     2401 gcatgcgcct gcaggacctg ctcgaaccgc tgcaatgcac cgggctcgcg caggtcgagc
     2461 cggatcgaca gccgctgcgc ctgctggtcg cagacctggg cgtagtccca gcagcgcctg
     2521 gcgcgcaggg cgaagccgcc gctgaattcg tcttcgcgca gcccgccttc caccaccagg
     2581 atgcggtcgc gggtcagcaa cggcgcgaat tcgtgccatt gctcgccgaa gaacgcgcat
     2641 tccaggcggc cacggccgtc ctcgagttgc acgaaggcct gcgaatcgcc gcgcttgcgc
     2701 atcccgacca cctgcccggc gaccaccgcc tgcatctccg ggcgccagcc gcgctgcgcg
     2761 tcctgcggcc gcgattccca caacgcgtcg agcgaggcca ggtcgtggcc gaccagccgc
     2821 ttcaggtcct cgcggtgcgg gtccagcggg tgcccgctga ggtaatggcc cagggtctcg
     2881 cgttcgccgt gcagcttctg cgccagcggc cattcgtcgc aggcaggcag gtcgatgtgg
     2941 atgtcgggcg cgccggtcgc gttgccgaac atgtcgacca tgccggcatc gcggttgcgg
     3001 gcgatctggt cggtggccct cagtacctcc ggcaactgca gcatcagcga ggcgcggttg
     3061 ccggccagtg catccatcgc gccggcatgg atcagtgcct cgagggtgcg cttgttgagg
     3121 ccggtggcgg ctacccgctt gcagaaatcg agcaggtccg cgaagcggcc gcctggttcc
     3181 cgtgccgcga cgatcgcctc gcaggcgccg cggccgacgc ccttcaccgc gcccaacccg
     3241 tagcggatgg tgccggggtc ggtggcctcg aacatatggg tcgagccgtt cacgtccggc
     3301 ggcagcaccc gcaggccgag cgcgcgcgcc tcgtcgagga agccgaccac cttgtcggtg
     3361 ttgtccatgt ccgaggacag caccgcggcc atgaactc
//
LOCUS       DAORUK010000189         3366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_38330_length_3366_cov_19.111145, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000189 DAORUK010000000
VERSION     DAORUK010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38330_length_3366_cov_19.111145"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PK818_09915"
     CDS             complement(<1..235)
                     /locus_tag="PK818_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83084.1"
                     /translation="MRIGTPIACLLVLVLAGCSPPEPPEEERRPEPQAEGTQAQPASA
                     IVQAADAYKGAARAAVANSEQAAAKEKAALDAAA"
     gene            299..1078
                     /locus_tag="PK818_09920"
     CDS             299..1078
                     /locus_tag="PK818_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY83085.1"
                     /translation="MNRNRWSLDGHRALITGASAGIGLAICRELLGLGAQVLMVARDA
                     ALLEQARADLEDEFPGPEDAVIAFPADVTDEEQRTELLDWVADHGGLHILVNNAGGNI
                     TRPALDYGEDQWRGIFETNLFSAFELCRGAYPLLTKHASSSIVNIGSVSGEVHVRSGA
                     PYGMSKAALHQMSRNLAVEWAEDGVRVNCVAPWYIRTRRTSGKLSDPDYLDEVLLRTP
                     LGRIGEPEEVAAAVAFLCMPASSYITGECIAVDGGFLRYGF"
     gene            complement(1066..1734)
                     /locus_tag="PK818_09925"
     CDS             complement(1066..1734)
                     /locus_tag="PK818_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83086.1"
                     /translation="MRKPAVIAAFALAVLLALAPRAASAQATLFYKCTNSRGEVSMQN
                     GTPCAAGMKQEVRRIGEVKTVPVPAKKPEPAAAAAAPVYGDFVLVSGPNMKRMPVPEA
                     ATLPPPPPLFQCRTWQGDTYFGESNAPAPRCVPLQVTGIDGSAGLGAGSACEMKYDAC
                     TATPAEQLCDAWLRRLDEAEFKLKYASRDDEAGRKAAFESIDAKVKASRCSPVAPEPP
                     AQNP"
     gene            complement(1734..2447)
                     /locus_tag="PK818_09930"
     CDS             complement(1734..2447)
                     /locus_tag="PK818_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HQY83087.1"
                     /translation="MTRIPALLLLLSAAAIVQAQATGEVRIYRCTDAAGTQTLRDTPC
                     PKGQQQSTRDMLRPKDAPTRARAPAPASVPAPRPVPAPVVYLAPPRPMYECTTHEGKR
                     YTSENGDGNPRWVPLWTLGYPMSGRPPHRDADRGSFRPAPPASAAAGRPQQALPTPAP
                     PPAHRHGGLPWPAASGGGTWIRDDCAMLPPAEACARLRDRRDQVRTRFFNAMPSERDV
                     LRVEERGINARLDNDCGGS"
     gene            complement(2511..3074)
                     /locus_tag="PK818_09935"
     CDS             complement(2511..3074)
                     /locus_tag="PK818_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HQY83088.1"
                     /translation="MAAACTVEEAVAALRRGGVIAYPTEAVWGLGCDPRDEAATLRLL
                     ALKQREVDKGLILVAADEAQLAPFIDMDVLDAAQRDAVRASWPGPHTWIVPASAGAPR
                     WITGAHNGIAVRVSAHPTVIALCNAFGSALVSTSANRAGAPAARSVAELDTRIVAAVD
                     AVAVGETGGLLRPTDIRDARTGSVLRL"
     gene            complement(3082..>3366)
                     /locus_tag="PK818_09940"
     CDS             complement(3082..>3366)
                     /locus_tag="PK818_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83089.1"
                     /translation="RKGFGAKKAAAKKVIAKKAPAVKKGTADAKPAKKAVKKTAAKKT
                     VKKAAAKKVAAKKAYVSPHPEPAKPLLTASSGEIGKKRIIKKPARDEAPF"
BASE COUNT          426 a         1188 c         1213 g          539 t
ORIGIN      
        1 gcgccgcggc gtcgagtgcc gccttctcct tcgctgcggc ctgctcgctg ttcgccacgg
       61 ccgcacgcgc ggcacccttg taggcatcag cggcctgcac gattgccgat gccggctgcg
      121 cctgcgtgcc ctcagcctgc ggttctggac gacgctcttc ttcgggtggc tctggcggcg
      181 agcatcctgc caataccagg accagcaggc aggcgatggg cgttccaatg cgcatggcgg
      241 actccagcgg tgcggacgtg gcctatccta cgccggaccc acaggatgga ggccgcgcat
      301 gaatcgcaac cgctggagcc tggacggaca ccgcgcgcta atcaccggcg ccagcgccgg
      361 catcggcctt gcgatctgcc gcgagttgct gggcctgggc gcgcaggtgc tgatggtggc
      421 gcgcgacgcg gccctgctgg agcaggcacg cgccgacctc gaggacgaat tcccggggcc
      481 ggaggacgcg gtgatcgcgt tccccgcgga cgtcaccgac gaagaacagc gcactgagtt
      541 gctggactgg gtggccgacc atggcggcct gcacatcctg gtcaacaatg ccggcggcaa
      601 catcacccgg ccggcgctgg actacggcga ggaccagtgg cgcggcatct tcgagaccaa
      661 cctgttctcc gcgttcgagc tttgccgcgg cgcgtatccg ctgctcacca agcacgcgtc
      721 gtcgagcatc gtgaacatcg gcagcgtgtc cggcgaggtc cacgtgcgca gcggtgcgcc
      781 gtacgggatg agcaaggccg cgctgcacca gatgagccgc aacctcgccg tcgaatgggc
      841 cgaggacggc gtgcgggtca actgcgtggc gccctggtac atccgcaccc gccgcacgtc
      901 cggcaagctg tccgatcccg attacctgga cgaggtgctg ctgcgcaccc cgctgggccg
      961 catcggcgaa ccggaggaag tggcggccgc cgtcgccttc ctgtgcatgc ctgcatccag
     1021 ctacatcacc ggcgagtgca tcgcggtgga tggcgggttc ctgcgttacg ggttctgagc
     1081 cggcggctcc ggcgccaccg gcgagcacct gctcgccttc acctttgcgt cgatcgattc
     1141 gaacgcggcc ttgcgcccgg cctcgtcgtc gcggctggcg tacttcagct tgaactcggc
     1201 ttcgtccagc cggcgcagcc aggcgtcgca cagctgttcg gcgggggtgg cggtgcatgc
     1261 gtcgtacttc atctcgcagg cgctgccggc acccaggccg gcgctgccgt cgatgccggt
     1321 gacctgcagc gggacgcaac gcggcgccgg cgcgttggac tcgccgaagt aggtatcgcc
     1381 ttgccaggtc cggcactgga acagcggcgg tggcggtggc agcgtggcgg cctctggcac
     1441 gggcatccgc ttcatgttcg gcccgctcac cagcacgaag tcgccgtaga ccggcgcggc
     1501 cgccgccgcg gccggctccg gcttcttcgc cggcaccggc accgtcttca cctcgccgat
     1561 ccgccggacc tcctgtttca tgccggccgc gcagggcgtg ccgttctgca tcgagacctc
     1621 gcccctggaa ttcgtgcact tgtagaacag cgtcgcctgc gcggatgccg cgcgcggcgc
     1681 cagcgccagc agcacggcca gcgcgaatgc ggcgatcacg gcaggcttgc gcatcaggaa
     1741 ccgccgcaat cgttgtcgag gcgcgcgttg atgccgcgtt cctcgacccg caggacgtcg
     1801 cgctcggacg gcatcgcgtt gaagaagcgc gtccggacct ggtcgcggcg atcgcgcagg
     1861 cgcgcgcagg cctcggccgg cggcagcatt gcgcagtcgt cgcggatcca ggtgccgccg
     1921 ccgctggcgg ccggccatgg cagcccgcca tggcggtgcg cgggtggcgg cgccggcgtc
     1981 ggcaaggcct gctgcggacg gccggccgcg gcgctggcag gcggcgcggg acggaagctg
     2041 ccgcgatcgg catcgcggtg cggcggccgc ccggacatgg ggtagcccag cgtccacagc
     2101 ggtacccagc gcggattgcc gtcgccgttt tcgctggtgt agcgcttgcc ctcgtgggtg
     2161 gtgcactcgt acatcggccg tggcggcgcc aggtacacca ccggtgcggg gaccggccgc
     2221 ggcgccggaa cggatgcggg tgccggcgcg cgcgcgcgcg tgggcgcatc cttcgggcgc
     2281 agcatgtcgc gggtggactg ttgctggccc ttcgggcacg gcgtgtcgcg cagggtctgc
     2341 gtgccggcgg catcggtgca gcggtagatg cgcacctcgc cggtggcctg cgcctgcacg
     2401 atggcggcgg ccgacagcag caggaggagg gcagggatcc gcgtcatgcg tgcatcttgc
     2461 gcatgcggcg ctgaaccgca agtttgcgcg ggcgaggctt gaacgcgggc tcagagacgc
     2521 agcacggagc cggtgcgcgc atcgcggatg tcggtcgggc gcagcaggcc gccggtctcg
     2581 cccacggcca cggcgtccac cgcagcgacg atgcgcgtat ccagttcggc gacggagcgt
     2641 gcggccggcg cgccggcgcg gttggcgctg gtcgatacca acgcgctgcc gaacgcgttg
     2701 cacagggcga tgacggtggg atgcgcgctg acccgcaccg cgatgccgtt gtgagcaccg
     2761 gtaatccatc gcggcgcgcc ggcggaggcc ggcacgatcc aggtgtgcgg ccccggccag
     2821 ctggcgcgca ccgcgtcgcg ttgcgcggcg tccagcacgt ccatgtcgat gaacggcgcg
     2881 agttgcgcct catcggcggc gaccaggatc aggcccttgt cgacctcgcg ctgcttcagc
     2941 gcgagcaggc gcagggtcgc ggcctcgtcg cgcggatcgc agccgagccc ccagaccgct
     3001 tcggtcgggt aggcgatgac gccaccgcgg cgcagcgcgg cgacggcttc ctcgacggtg
     3061 caggccgcgg ccatcaacgg ttcagaacgg cgcctcgtcg cgcgccggct tcttgatgat
     3121 gcgcttcttg ccgatctcgc cgctgcttgc ggtcagcagc ggcttggccg gctccggatg
     3181 cgggctgacg taggccttct tcgccgcgac tttcttcgcc gcggccttct tcaccgtctt
     3241 tttcgccgcc gtcttcttca ctgccttttt cgccggcttg gcgtcggccg tgcccttctt
     3301 cactgccggc gccttcttcg cgatgacctt cttcgccgcc gccttcttcg cgccgaagcc
     3361 cttgcg
//
LOCUS       DAORUK010000190         3338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_38867_length_3338_cov_19.145294, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000190 DAORUK010000000
VERSION     DAORUK010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38867_length_3338_cov_19.145294"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1129)
                     /locus_tag="PK818_09945"
     CDS             complement(77..1129)
                     /locus_tag="PK818_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014506875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQY83090.1"
                     /translation="MTEATAPPSICLLRLSALGDVTHVLPLLRTLRKAWPQAAIAWVI
                     GKGEHRLLAGLPGVRFIEYDKQSGLGGMRTVRRQCGGRFDVLLQLQVSGRANLLSAFI
                     PARRRIGYDRSRSKDGHGLFINERIADRPGLHVLDAIGSFADALGLQREAVAWDLPVP
                     GEAHAWARAQWDDDGRRTLMVSPCSSHALRNWRAERHAALADHAAAQGWRIVLCGGRS
                     ALERETGDAILAAMRARDGVLDLIGKDTLKQLPALLARADLLVTPDSGPMHIANAMGT
                     KVLGLHAASNPHRSGPYSDRRYCVDRYDDAARRYRGKPAAALKWGSKIEAEGVMDLVA
                     VDDAVAAFERYRSDFC"
     gene            1150..1893
                     /locus_tag="PK818_09950"
     CDS             1150..1893
                     /locus_tag="PK818_09950"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019305198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HQY83091.1"
                     /translation="MTGFDANEHLTPFRDARGYGAILFDRTQLRQPDPAWFAPAHWGE
                     AAQPVAEGGRGGAWFIETGEGDALLRQYLRGGWAASLSRDGHLWRGINRVRSFAEFRL
                     LRALREKKLPVPLPFAAYYRREGMHYRAAILMQRLPGVRSLAALAATGEAPWEAAGQL
                     VAKFHRAGLEHADLNAHNILFDAQGRGWMIDFDRSQLRIPATRWRSANLERLQRSLRK
                     RRGARSIEQVDADFAKLRGAYDTAWQRGY"
     gene            1895..2641
                     /locus_tag="PK818_09955"
     CDS             1895..2641
                     /locus_tag="PK818_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQY83092.1"
                     /translation="MSGWSLRLLGVGNASAVELGSAMATIERDGAPWLTIDCGGEGLT
                     AYQRAYGGIPAALFMTHAHMDHIAGFERLFVAGWFDPAHRGEVRLYVPATLLPLLQQR
                     VASYPNALAEGGVNFWDAFRVVPVGDHFWHDGLRFEVFPVRHHWPETAFGLRLRGSVV
                     WTGDTRPIPEMLAKFADAGELIAHDCALEGNPSHSGIDDLEREYPPALLARCMLYHYA
                     SAEDGEALRARGHRVGMPGEVVPLHAPVVA"
     gene            complement(2664..2760)
                     /gene="ffs"
                     /locus_tag="PK818_09960"
     ncRNA           complement(2664..2760)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK818_09960"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2801..2893)
                     /locus_tag="PK818_09965"
     tRNA            complement(2801..2893)
                     /locus_tag="PK818_09965"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2857..2859),aa:Ser,seq:gga)
     gene            2984..>3338
                     /locus_tag="PK818_09970"
     CDS             2984..>3338
                     /locus_tag="PK818_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQY83093.1"
                     /translation="MSYLVLARKWRPRRFAELVGQEHVVRALTNALETGRVHHAFLFT
                     GTRGVGKTTIARIFAKSLNCERGTSAEPCGECETCLAIDAGRYIDLLEIDAASNTGVD
                     NVRELIENAQYMPSRG"
BASE COUNT          499 a         1199 c         1159 g          481 t
ORIGIN      
        1 catcaacctg gcgcttggct cggcctacgt cgaggaaaag tcctaccagg actacggcag
       61 cgggcagggc gcctgatcag cagaagtcgc tgcgatagcg ctcgaacgcg gccaccgcgt
      121 cgtcgaccgc caccaggtcc atcaccccct cggcttcgat cttgctgccc cacttgagcg
      181 cggccgccgg cttgccacgg tacctgcgcg cggcatcgtc atagcggtcc acgcagtagc
      241 ggcgatccga gtacggtccg ctgcgatgcg ggttgctggc cgcgtgcagg cccagcacct
      301 tggtccccat cgcgttggcg atgtgcatcg gccccgaatc cggggtcacc agcaggtcgg
      361 cgcgggccag cagcgccggc agctgcttga gggtgtcctt gccgatcagg tccagcaccc
      421 cgtcgcgcgc gcgcatcgcg gcgaggatcg catcgccggt ctcgcgttcc agcgcgctgc
      481 ggccgccgca cagcacgatg cgccagccct gcgccgcggc gtggtcggcc agcgcggcat
      541 gccgctccgc gcgccagttg cgcagcgcat ggctggagca gggcgagacc atcagcgtgc
      601 gccggccgtc gtcgtcccat tgcgcgcgcg cccatgcatg cgcctcgcca ggcaccggca
      661 ggtcccaggc gaccgcctcg cgctgcagtc ccagcgcgtc ggcgaagctg ccgatcgcgt
      721 ccagcacgtg caggccgggg cggtcggcga tgcgttcgtt gatgaacagc ccgtggccgt
      781 ccttcgaacg cgagcggtcg tagccgatcc gccgccgcgc cgggatgaac gccgacagca
      841 ggttggcgcg tcccgagacc tgcaactgca gcagcacgtc gaagcgaccg ccgcattgcc
      901 ggcgcaccgt gcgcatgcca ccaagcccgg actgcttgtc gtactcgatg aagcgcacgc
      961 cgggcaggcc ggccagcaag cggtgctcgc ccttgccgat cacccaggcg atcgcggcct
     1021 gcggccatgc cttgcgcagc gtgcgcagca gtggcaacac atgggtgaca tcgcccagcg
     1081 cggacaggcg cagcaggcag atcgaaggcg gcgcggtggc ttcggtcacg tgcttgctaa
     1141 actcggttga tgacgggctt cgacgccaac gaacacctga cgccattccg cgatgcgcgc
     1201 ggctacgggg cgattctgtt cgaccgcacg cagttgcggc aacccgaccc cgcctggttc
     1261 gcgccggcgc actggggcga ggctgcgcag ccggtcgccg aaggcggccg cggcggcgcc
     1321 tggttcatcg aaaccggcga gggcgacgcg ctgctgcgcc agtacctgcg cggcggctgg
     1381 gccgccagcc tgagccgcga cggtcacctg tggcgcggca tcaaccgcgt gcgcagcttc
     1441 gccgagttcc gcctgctgcg cgcgctgcgc gagaagaagc tgccggtgcc gctgccgttc
     1501 gccgcctact accggcgcga aggcatgcac tatcgcgccg cgatcctgat gcagcggttg
     1561 cccggggtgc gctcgctggc ggcgctggcc gccaccggcg aggcgccatg ggaagcggcc
     1621 ggccagctgg tcgcgaagtt ccatcgcgcc ggcctcgagc atgccgacct caacgcgcac
     1681 aacatcctgt tcgatgcgca ggggcgcggc tggatgatcg acttcgaccg ctcgcagctg
     1741 cggatcccgg ccacccgctg gcgcagcgcc aacctcgagc gcctgcagcg gtcgctgcgc
     1801 aagcgccgcg gcgcgcgcag catcgagcag gtcgacgccg atttcgcgaa actgcgcggc
     1861 gcctacgaca ccgcgtggca acgggggtac tgagatgagc ggatggagcc tgcgcctgct
     1921 cggggtcggc aacgcgtcgg cggtcgagct gggctcggcg atggccacca tcgagcgcga
     1981 tggcgcgccg tggctgacca tcgactgcgg cggcgaaggc ctgaccgcct accagcgcgc
     2041 ctacggcggc attcccgccg cgctgttcat gacccatgcg cacatggacc acatcgccgg
     2101 cttcgagcgc ctgttcgtgg ctggctggtt cgacccggcg catcgcggcg aagtgcgcct
     2161 gtacgtgccg gcgacgctgt tgccgctgct gcagcagcgc gtggccagtt acccgaacgc
     2221 actggccgag ggcggggtga atttctggga tgcgttccgg gtggtcccgg tgggcgacca
     2281 cttctggcac gacggcctgc gcttcgaggt gttcccggtc cgccaccact ggccggagac
     2341 ggcgttcggc ctgcgcctgc gtggcagcgt ggtatggacc ggcgataccc ggccgatccc
     2401 ggaaatgctg gcgaagttcg ccgatgccgg cgagctcatc gcgcatgact gcgcgctgga
     2461 gggcaacccc tcgcacagcg gcatcgacga cctcgagcgc gaatacccgc ccgcgctgct
     2521 cgcccgctgc atgctctacc actacgccag cgcggaagac ggcgaggcgc tgcgcgcgcg
     2581 cggccaccgg gtcggcatgc cgggcgaggt ggtgccgctg cacgccccgg tcgttgcctg
     2641 accgcggtcg cggaaaggcg gtggccccgc cggctggcct cggcacccgc gtcaatcgat
     2701 accgttgctg ccttccggcc ctggcggagt tttcgatcta gcgtcgcgag gggccgacgg
     2761 ggccaccatg gaaaaccgcg tcgcgggcga cgcgcaaagc tggcggagag agggggattc
     2821 gaacccccga agcgcggttt agacgcttac acactttcca ggcgtgctcc ttcaaccact
     2881 cggacacctc tccggaaccg cccattctag cggcggtcgc gggtcgccac aaggaagccc
     2941 tggcggcgcg accggggcag tggcacgcat gtcagaatga gccatgtcct atctcgtgct
     3001 cgcccgcaag tggcgcccca ggcgtttcgc cgaactggtc ggccaggaac acgtggtgcg
     3061 cgcgctgacc aatgcgctgg aaaccggccg cgtccaccac gcgttcctgt tcaccggcac
     3121 ccgcggcgtc ggcaagacca ccatcgcccg gatcttcgcc aagagcctga attgcgagcg
     3181 cgggaccagc gccgaaccct gcggcgaatg cgagacctgc ctggcgatcg acgccggccg
     3241 ctacatcgac ctgctggaga tcgacgccgc ctccaacacc ggtgtggaca acgtccgcga
     3301 actgatcgag aacgcgcagt acatgccgtc gcgcggca
//
LOCUS       DAORUK010000191         3338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_38868_length_3338_cov_15.260433, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000191 DAORUK010000000
VERSION     DAORUK010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38868_length_3338_cov_15.260433"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PK818_09975"
     CDS             <1..546
                     /locus_tag="PK818_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83094.1"
                     /translation="AIHQAVVVSNGRDAYTGEPLEWGLISTYDNDASKNGKHHYKATF
                     ALLPTVDHVEASAKTASFKVCAWRTNDAKNDLSHESFVELCKRVLEHEGHLVTRKNNT
                     LRKRKPQSAITTASGYRNRNGQVVVRRTELPGNDHLQRIYVLRCEKCSAQYGANGCDI
                     HLRRCPHCDAGQPGLDFGHAD"
     gene            complement(562..1542)
                     /locus_tag="PK818_09980"
     CDS             complement(562..1542)
                     /locus_tag="PK818_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQY83095.1"
                     /translation="MTLTQLRYLVAIADSRLNITQAAERVHATQPGLSKQLKQLEDEL
                     GFQLFTRRGRSLESIAPAGVRVIEHARKVLAEVANIRSYAANERGEYSGRLLLATTHT
                     QARYVLPPAIAAIKREFPQVSVDLRAASESDVLGQLDQADLALISTTGSVPQGGMAVP
                     LFRWRRVLLVPDTHPLAGLQRAPTLAELGRHPLISYESSTRADSSLQRAFAGASVTPQ
                     IAMTARDANLIKTYVRAGLGAGLLAEMAVTHGEDGDLRIIPAPAEIPECITWAVIPRG
                     RVLRDYALGLLHWLAPQLDRRDLRRVLEGNQDPNWPQPPGWAELAQSISV"
     gene            1692..3134
                     /gene="cysG"
                     /locus_tag="PK818_09985"
     CDS             1692..3134
                     /gene="cysG"
                     /locus_tag="PK818_09985"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HQY83096.1"
                     /translation="MSPHPPALFPLFADLRGRRVLVVGGGAVARRKAEALLQAGARVL
                     VGAPVLEPQLAQWLAAARIEHLAGTFAPEWLDAAWLVIAATDDVATNRAVVEAADARR
                     IFANAVDDAEMSRFHVPARVERGPLQIAISSGGGAPMLARLVRERLELQFDESFGALA
                     DLLARNRQRIRARWPGLDARRRGFAGLLSGPLQGLLRKRQHLAAERAFDDALRGDAPP
                     ARGSVALVGAGPGDPGLLTLRALRLLNEADVILHDRLVSDEVLQLARRDAERISVGKH
                     AGDGGHSQAQINALLVALAAAGKRVVRLKGGDPFVFGRGGEELQALRAHAIDCEVVPG
                     ITAALACAAHAGIPLTHRDHAQALQLVTAHGRDDIDALDWSALARPRQTLAFYMGVAA
                     LDQVQAQLLAHGMPATTPFAIVENGSRAAQRVVAGALADLPALARAHAVRAPALLIVG
                     EVAAHACSLHWFGREPLTTADAASPLLAAA"
     gene            3157..>3338
                     /locus_tag="PK818_09990"
     CDS             3157..>3338
                     /locus_tag="PK818_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQY83097.1"
                     /translation="MALYDNILDTIGNTPIVRLQRIAPKHVTLYAKVESFNPGGSVKD
                     RLALAIVLDAEQRGLL"
BASE COUNT          533 a         1149 c         1134 g          522 t
ORIGIN      
        1 gcaatccacc aagccgtcgt ggtatcgaat gggcgagacg cgtacaccgg agaacccttg
       61 gaatggggcc tgatcagcac ctatgacaac gacgcatcca agaacggcaa gcatcattac
      121 aaggccacct tcgcgcttct acccaccgtc gatcacgtag aagcaagcgc gaaaaccgcg
      181 tcattcaagg tatgcgcctg gcgcacgaat gatgcaaaga acgatttgag ccacgaatca
      241 ttcgtggagc tttgcaagcg tgtgcttgag catgaaggcc atctcgtaac tcgcaaaaac
      301 aacacacttc gcaaacgaaa gccgcaaagc gccattacga ccgcatctgg ctaccgcaat
      361 cgcaatggcc aagtagtagt gcgccgcacg gagttgcccg gaaacgatca cctgcaacgc
      421 atttatgtgc tgcgatgcga gaaatgctca gcgcaatacg gcgcaaacgg atgcgacatc
      481 cacttgagac gatgcccgca ttgtgacgct ggccaaccgg gcttggactt cgggcatgcg
      541 gattgacgac cgcctggcca cctagacgct gatcgactgc gccagctccg cccagcccgg
      601 cggctgcggc cagttcggat cctggttgcc ttccagcacc cgccgcaggt cgcggcggtc
      661 gagttgcggc gccagccagt gcagcaggcc cagtgcgtag tcgcgcagca cgcggccgcg
      721 cgggatcacc gcccaggtga tgcattcggg gatttccgcg ggcgccggga tgatccgcag
      781 gtcgccgtct tcgccgtggg tcaccgccat ctccgccagc aggccggcgc ccagcccggc
      841 gcgcacgtag gtcttgatca ggttggcgtc gcgcgcggtc atcgcgatct gcggggtgac
      901 gcttgcgccg gcgaacgcgc gttgcagcga ggaatccgcg cgggtcgagc tctcgtagct
      961 gatcagcggg tgccggccga gttcggccag ggtcggcgcg cgctgcaggc cggccagcgg
     1021 gtgcgtgtcc ggcaccagca atacccgccg ccagcggaac agcggcaccg ccatgccacc
     1081 ctgcggcacg ctgccggtgg tgctgatcag ggcgaggtcg gcctggtcga gctgcccgag
     1141 cacgtcggac tcactggcgg cccgcaggtc cacgctgacc tgcgggaatt cgcgcttgat
     1201 cgccgcgatt gccggtggca gcacgtagcg cgcctgggtg tgggtggtcg caagcagcaa
     1261 ccggccgctg tactccccgc gctcgttggc ggcatagctg cggatgttgg cgacctccgc
     1321 cagcaccttg cgcgcatgct cgatcacccg cacgccggcc ggggcgatgc tctccaggct
     1381 gcggccacgg cgggtgaaca gctggaagcc gagttcgtcc tccaattgct tgagctgctt
     1441 ggacaggccg ggctgggtgg cgtgcacgcg ctcggccgcc tgggtgatgt tcagccggga
     1501 atcggcaatc gcgaccaggt agcgcagctg ggtcagtgtc acggcgggat cctttcatcg
     1561 ctatatgcgt attgagcgat gaatatatcg atgggctgca ggcctgtcca gacggacttt
     1621 atgccttgcc ggcataagca aagacgccag cgccattttc cgcaaccgct gccacgccct
     1681 agcgtcaccg catgtccccg cacccgcccg ccctgttccc cctgtttgcc gacctgcgcg
     1741 gccgccgcgt gctggtggtc ggcggcggcg cggttgcacg gcgcaaggcg gaagcgctgc
     1801 tgcaggccgg tgcccgggta ctggtcggcg cgccggtgct ggagccgcaa ctggcgcagt
     1861 ggctggcggc ggcccggatc gaacaccttg caggcacctt cgcgccggag tggctggacg
     1921 cggcctggct ggtgatcgcg gccaccgacg atgtcgcgac caatcgcgcc gtggtcgaag
     1981 ccgccgatgc gcgccggatc ttcgccaatg cggtggacga tgccgagatg tcccgcttcc
     2041 acgtgccggc gcgggtcgaa cgcggcccgc tgcagatcgc gatttccagc ggtggcggtg
     2101 caccgatgct ggcgcggctg gtgcgcgaac ggctggaact gcagttcgac gaatccttcg
     2161 gtgcgctggc ggacctgctg gcgcgcaatc gccagcgcat ccgcgcacgc tggcccgggc
     2221 tcgatgcgcg caggcgcggc ttcgccggcc tgctgtccgg cccgttgcag ggcctgctgc
     2281 gcaaacgcca gcacctcgcc gccgagcgcg cgttcgacga cgcattgcgc ggcgacgccc
     2341 cgcccgcgcg gggctcggtg gcgctggtcg gcgccggccc cggcgacccc gggctgctca
     2401 ccctgcgcgc gctgcgcctg ctcaacgaag ccgacgtgat cctgcacgac cgcctggtca
     2461 gcgatgaggt cctgcaactg gcgcgccgcg acgccgagcg catcagcgtc ggcaagcacg
     2521 ccggcgacgg cgggcactcg caggcgcaga tcaatgcgtt gctggtcgcg cttgccgcgg
     2581 ccggcaagcg ggtggtgcgg ctgaagggcg gcgacccgtt cgtgttcggc cgcggcggcg
     2641 aggaactgca ggcactgcgt gcgcacgcga tcgactgcga agtggtgccc ggcatcaccg
     2701 cggcgctggc gtgcgccgcg catgccggga tcccgctgac ccatcgcgac cacgcgcaag
     2761 cgctgcaact ggtcaccgcg catggcaggg acgacatcga cgcactcgac tggtccgcgc
     2821 tggcgcggcc gcggcagaca ctggcgttct acatgggcgt ggccgcgctg gaccaggtcc
     2881 aggcgcaact gttggcgcat ggcatgcctg caaccacacc cttcgcgatc gtcgagaacg
     2941 gcagccgcgc cgcgcagcgc gtggtcgcgg gtgcgctggc cgacctgcct gcgctggcgc
     3001 gtgcacacgc tgtccgcgcg ccggcgctgc tgatcgtggg cgaggtcgcc gcgcatgcat
     3061 gcagcctgca ctggttcggt cgcgagccct taacaaccgc ggatgcagca tcacccctgc
     3121 tggcagccgc ctgaacttcc actaccggac cttcccatgg ccctttacga caacatcctc
     3181 gacaccatcg gcaacacccc catcgtcagg ctgcagcgca tcgcgccgaa gcacgtgacg
     3241 ctgtatgcga aggtcgaatc cttcaacccc ggcggctcgg tcaaggaccg gctggcgctt
     3301 gcgatcgtcc tcgacgccga gcagcgcggg ctgctgaa
//
LOCUS       DAORUK010000192         3318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_39240_length_3318_cov_22.551946, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000192 DAORUK010000000
VERSION     DAORUK010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39240_length_3318_cov_22.551946"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PK818_09995"
     CDS             complement(<1..866)
                     /locus_tag="PK818_09995"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HQY83098.1"
                     /translation="MNHRRFNALAAAITLSLVATGAQAAGKVDLHKQDLNQLKQQYNA
                     AVAAKGAVAPMAHARHAQLIGADANTHLLMKAKREDYGVRNYRYNQSFRGVPIFGEGV
                     VVSEDSAGNVRALFGNKVTGLERDIGSTTPRLSKAAALVAGKRAGLGNRISGMLTSNE
                     KTELVIYVDDGGHGHLAYHVDFFADSATGGAPTRPSVLVDANSGRVLKQWENLQHALV
                     GTGPGGNSKTGQYEYGTNFGYLDVTQSGTTCTMNNTNVKTVNLNNGSTNTSTTAFSYT
                     CPRNTVKAINGA"
     gene            complement(1316..1867)
                     /gene="sufT"
                     /locus_tag="PK818_10000"
     CDS             complement(1316..1867)
                     /gene="sufT"
                     /locus_tag="PK818_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003488784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HQY83099.1"
                     /translation="MYSRSSEPVRFERDCAAVLVPQGDQVTLPAGTVGYITQSLGGSW
                     TVFVEGNLMRIAGNDADAIGKQPPESIELPEGASDEDVEAMVWRQLRTCFDPEIPVNI
                     VELGLVYSAEVGTREDGTREVAVRMTLTAPGCGMGEILVDDVRSKIELIPTVAEADVE
                     LVFDPPWNRTMMSELAQLETGLL"
     gene            1962..2402
                     /locus_tag="PK818_10005"
     CDS             1962..2402
                     /locus_tag="PK818_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83100.1"
                     /translation="MTTNPSRTPLTALLLLLCGSLGFAAAWVLAMRALDTHAAWLAPL
                     AALDAVLMLRLGRARPGVANAALALAATAASILLANWGIAATEIGRSLGLLPWESALR
                     LGPAYAWELIRLANGPAQLGWYALALVVAVFGGLSGRRPAPSVR"
     gene            complement(2368..>3318)
                     /locus_tag="PK818_10010"
     CDS             complement(2368..>3318)
                     /locus_tag="PK818_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HQY83101.1"
                     /translation="DGRMDKRYTFPGQQWFNAAVAVAAVVCGAMALQTLAMPWIVAFF
                     VLALALGVLMTLPIGGADMPVVISLYNAFTGLAVAFEGYVLGNEAMIIAGMMVGAAGI
                     LLTRLMAKAMNRSIKSVLFSDFGGSSGEMQAIGGSMKPIEASDVAAMMAFAERVVIVP
                     GYGLAVAQAQHKIWELTQRLQERGVKVKFAIHPVAGRMPGHMNVLLAEAGVPYDLIAD
                     MDDINPEFANTDVSLVIGANDVVNPVAKTDPASPIYGMPILDVVNSKNTIVIKRGKGT
                     GFAGIENALFYADNTRMLYGDGSEMANALVSELKALDGGH"
BASE COUNT          478 a         1110 c         1147 g          583 t
ORIGIN      
        1 taggcgccgt tgatggcctt caccgtgttg cgcgggcagg tgtaggagaa cgcggtggtg
       61 ctggtgttgg tgctgccgtt gttgaggttg acggtcttga cgttggtgtt gttcatcgtg
      121 caggtggtgc cgctctgcgt gacgtccagg tagccaaagt tggtgccgta ctcgtactgg
      181 ccggtcttgc tgttgccgcc ggggccggtg ccgaccagcg cgtgctgcag gttttcccac
      241 tgcttgagca cgcggccgct gttggcgtcg accagcacgc tgggacgggt cggcgcgccg
      301 ccggtggcgg aatcggcgaa gaagtcgacg tggtaggcca ggtggccatg gccgccgtca
      361 tcgacgtaga tgaccagttc ggtcttctcg ttggaggtca gcatgccgga gatgcggttg
      421 ccaaggccgg cgcgcttgcc ggcgaccagc gcggccgcct tcgacaggcg cggggtggtc
      481 gagccgatgt cgcgctccag gccggtgacc ttgttgccga acagcgcgcg cacgttgccg
      541 gcgctgtcct cgctgaccac cacgccctcg ccgaagatcg gcacgccgcg gaagctctgg
      601 ttgtagcggt agttgcgaac cccgtagtcc tcgcgcttcg ccttcatcag caggtgcgtg
      661 ttcgcgtcgg cgccgatcag ctgggcgtgg cgtgcgtgcg ccatcggggc gaccgcgccc
      721 ttggcggcaa ccgcggcgtt gtactgctgc ttgagctggt tcaggtcctg cttgtgcagg
      781 tcgaccttgc ccgcggcctg cgcgccggtg gcgacaagcg aaagggtgat ggcggccgca
      841 agggcattga agcgacgatg gttcactggt tgattccccg aggatggttg gcggtggacc
      901 gcacgcggcg gccgggctgg catggcacac aggtcccgct gggccggtcg gcgcaagcgc
      961 cggtcgagcg caacaacaat gcgggagtcg cctcaggaag ctgaaccaaa cccgaagcga
     1021 tgcgggagcg tcggggccag tgtgacggcc gtcaagaagg caccgtcatg cgcccataac
     1081 atttcggcat aagcgagcgt gaaatcgtag gcaatggcgt tccagagcca ttcctcgatt
     1141 tgctgcaacg cgccaagcag ccccggagtg tgcgccagtc gacagcaaat gctgcggggc
     1201 accaagaatt cgcgcagcgc gaagccgcca aaagtgacgc tgagcggcag tgccgatggt
     1261 cacgaagccg tgaacggtgt cacgcaagcc tgtccgctgc caggccctgg ccgcgttaga
     1321 gcagcccggt ttccagctgc gccagctcgg acatcatggt gcggttccac ggcgggtcga
     1381 acaccagttc gacatcggct tcggccacgg tcgggatcag ttcgatcttg ctgcgcacgt
     1441 cgtcgaccag gatttcgccc atgccgcagc cgggtgcggt cagggtcatg cgcacggcaa
     1501 cctcgcgggt gccgtcctcg cgggtgccga cctcggccga atacaccagc cccagctcga
     1561 cgatgttgac cgggatctcg gggtcgaagc aggtccgcag ctggcgccac accatcgctt
     1621 ccacgtcctc gtcgctggcg ccttcgggca gctcgatgga ttccggcggc tgcttgccga
     1681 tggcgtcggc gtcgttgccg gcgatccgca tcaggttgcc ttcgacgaac accgtccagc
     1741 tgccgcccag cgactgggtg atgtagccga cggtccccgc gggcagggtg acctggtcgc
     1801 cctgcggcac cagcacggcc gcgcaatcgc gctcgaaacg gacgggttcg ctgctgcgtg
     1861 agtacatgcg gtcgggtggc acggcgtgcg tcggggaatt cgtaccattc tagtcccttt
     1921 ccgcactggc tatcatgcgc gacccccgat cgccgccggc catgaccacg aatccatccc
     1981 gcacgccgct gaccgccctg ctgctcctgc tgtgcggcag cctcgggttc gccgcggcgt
     2041 gggtgctggc gatgcgcgcg ctggacaccc atgccgcctg gctggcgccg ctggcagcgc
     2101 tggatgcggt gctgatgctg cgcctgggcc gggccaggcc gggcgtggcc aacgcggcac
     2161 tggcgctggc cgccaccgcg gccagcatcc tgctggcgaa ctggggcatc gccgccaccg
     2221 agatcggccg cagcctgggc ctgctgccgt gggaatccgc gctgcggctg ggcccggcct
     2281 acgcctggga gctgatccgg ctggccaacg gcccggcgca gctgggctgg tacgcgctgg
     2341 cgctggtggt ggcggtgttc ggcggcctca gtggccgccg tccagcgcct tcagttcgct
     2401 gaccagcgcg ttcgccatct cgctgccgtc gccgtacagc atgcgggtgt tgtcggcgta
     2461 gaacagcgcg ttctcgatgc cggcgaagcc ggtgcccttg ccgcgcttga tcacgatggt
     2521 gttcttcgag ttgaccacgt cgaggatcgg catgccgtag atcgggctgg ccgggtcggt
     2581 cttggccacc gggttgacca cgtcgttggc gccgatcacc aggctcacgt cagtgttggc
     2641 gaattccggg ttgatgtcgt ccatgtcggc gatcaggtcg tagggcacgc cggcttcggc
     2701 cagcagcacg ttcatgtggc cgggcatgcg cccggcgacc gggtggatcg cgaacttcac
     2761 cttcaccccg cgttcctgca ggcgctgggt cagctcccag atcttgtgct gggcctgcgc
     2821 caccgccagc ccgtagccgg gcacgatcac cacccgctcg gcgaacgcca tcatcgccgc
     2881 cacgtcgctg gcctcgatcg gcttcatcga gccgccgatg gcctgcattt ccccgctgct
     2941 gccgccgaaa tcggagaaca gcacgctctt gatgctgcgg ttcatcgcct tcgccatcag
     3001 ccgggtcagc aggatgccgg ccgcgccgac catcatgccg gcgatgatca tcgcctcgtt
     3061 gcccagcacg taaccctcga acgcgaccgc cagcccggtg aaggcgttgt acagcgagat
     3121 caccaccggc atgtcggcgc cgccgatcgg cagcgtcatc agcacgccca gcgccagcgc
     3181 cagcacgaag aacgccacga tccacggcat cgccagggtc tgcagggcca tcgcgccgca
     3241 caccactgcc gccaccgcga ccgccgcgtt gaaccactgc tggccgggga aggtgtagcg
     3301 cttgtccatg cgcccgtc
//
LOCUS       DAORUK010000193         3311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_39389_length_3311_cov_16.548219, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000193 DAORUK010000000
VERSION     DAORUK010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39389_length_3311_cov_16.548219"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PK818_10015"
     CDS             complement(<1..874)
                     /locus_tag="PK818_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1704 domain-containing protein"
                     /protein_id="HQY83102.1"
                     /translation="MEGTEPTASAFAPAIAHHAALDARMAKAARAIRLLSLVSWPAGE
                     QQAFLADFARGQARLPAHQYPKHDFSDERREFDAIAAAADPAHPLGVYLIESARSWAV
                     AAELLENLGSPRVTELSVALFGKPDYTLPGGATTREAANHFIASADELDRELLAPSEQ
                     VAISATSLGMQLQAALDDYFDARVISVELDPELIAKAAAGPNRIRLRAGAAFSDYDLH
                     QLLQHEAFVHSLTALNGQQQAVLPSMALSSPRTTATQEGLATFAEQITGSIDIERMKR
                     VSLRIEAVAMALDGA"
     gene            complement(861..1961)
                     /gene="rsgA"
                     /locus_tag="PK818_10020"
     CDS             complement(861..1961)
                     /gene="rsgA"
                     /locus_tag="PK818_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HQY83103.1"
                     /translation="MTPLQAIGWRWTQASLDPAWAELMAAHPQARQARISEQHRSGYL
                     VAESVDASHRAESLPEWQRGGSYRRGEITPESRPAVGDWVLVEGEAPNALRIVALLPR
                     FSAIKRGAAGEHYKQQVIAANIDTVFVVCGLDADFNPRRIERYLLLVGGSGVQPVVVL
                     TKSDKEGADVEAALQELLALGVPVLAVNAKDRASVAGLEPWLGEGQSIVLVGSSGAGK
                     STLTNTLLGLEKMKTGAVRAGDDRGRHTTTHRALIALPSGACMIDTPGMRELKPTGEE
                     VVAESFADIEALAASCKFNDCRHQQEPGCAVREAIASGAADARRVASFFKLSSEVAGA
                     ANKLATRLAQKADAKVGNKAFYKRVDDKYGRH"
     gene            2137..>3311
                     /locus_tag="PK818_10025"
     CDS             2137..>3311
                     /locus_tag="PK818_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQY83104.1"
                     /translation="MPPPTLQTRTRLSEVRYEIRGELARRARELEAQGRTLIKLNIGN
                     PGAFGFRAPEHLQRAIADHIARTDPYTHQQGLPEAREAIAAFHRQRGTPNASPERVFI
                     GNGVSELIDISLRALLNPGDEVLLPSPDYPLWSAATILNDGRPVYYRCDEANGFLPDP
                     EQIESLVSPRTRAIVLINPNNPTGANYPRALLERIVAIAAKHRLLLMADEIYDGILYD
                     DHSFQPLAPLAGDLPCLSFGGLSKVHRACGWRVGWAVLSGDPLASGDYHHAMDLLGAL
                     RLCANVPGQFAIEQALHGADTIGELVRPGGRLYETRKALVESCAASEHLSLVAPAGAL
                     YGFPAIVGDAAHGLDDHAFALEMLETEDVLIVPGSSFNVPYRNHFRVTLLPEAPMLR"
BASE COUNT          471 a         1229 c         1100 g          511 t
ORIGIN      
        1 ccgcgccgtc cagcgccatc gccaccgcct cgatccgcag gctgacccgc ttcatgcgct
       61 cgatgtcgat gctgccggtg atctgctcgg cgaaggtggc cagcccttcc tgggtggcgg
      121 tggtgcgcgg cgaggacagc gccatgctcg gcagcaccgc ctgctgctgg ccgttgagcg
      181 cggtcagcga gtgcacgaag gcctcgtgct ggagcaactg gtgcaggtcg tagtcggaga
      241 acgcggcgcc ggcgcgcagg cggatccggt tcggccccgc cgcggccttg gcgatcagct
      301 ccgggtccag ttcgacgctg atcacccgcg cgtcgaagta gtcgtccagc gcggcctgca
      361 gctgcatccc cagcgaggtc gccgagatcg ccacctgctc ggacggcgcc agcagctcgc
      421 ggtcgagttc gtcggcgctg gcgatgaagt ggttggccgc ctcgcgggtg gtggcgccgc
      481 ccggcagggt gtagtcgggc ttgccgaaca acgccaccga aagctcggtg acccgcggcg
      541 agccaaggtt ctccagcagt tccgccgcga ccgcccagct gcgcgcggat tcgatcaggt
      601 acaccccgag cggatgcgcg ggatcggcgg cggcggcgat cgcgtcgaac tcgcggcgtt
      661 cgtcggagaa atcatgcttc gggtactggt gcgccggcag ccgcgcctgc ccgcgcgcga
      721 agtcggcgag gaaggcctgt tgctcgccgg ccggccagct gaccaggctg agcaggcgga
      781 tcgcccgcgc cgccttcgcc atccgcgcat ccagcgcggc gtggtgggcg atggcggggg
      841 cgaatgcgga ggcggtcggc tcagtgcctt ccatacttgt cgtccacgcg cttgtagaag
      901 gccttgttgc cgaccttcgc gtccgccttc tgggcaagcc gggtggccag cttgttggcg
      961 gcgccggcca cctcgctgct gagcttgaag aaactggcca cccgccgcgc atccgccgcg
     1021 ccgctggcga tcgcctcgcg cacggcgcag ccgggttcct gctggtgccg gcagtcgttg
     1081 aacttgcagc tggcggccag tgcctcgatg tcggcgaagc tttcggccac cacttcctcg
     1141 ccggtcggct tcagttcgcg catgccgggg gtatcgatca tgcacgcccc ggacggcagt
     1201 gcgatcaacg cgcgatgggt ggtggtgtgg cggccgcggt cgtcgcccgc gcgcaccgcg
     1261 ccggtcttca tcttctcgag gccaagcagg gtgttggtca gcgtggactt gccggcgccg
     1321 gaactgccga ccaggacgat gctctggccc tcgcccagcc acggttccag gccggccacg
     1381 ctggcgcgat ccttggcatt caccgccagc accggcacgc ccagcgcgag cagttcctgc
     1441 agcgcggcct cgacgtcggc accctccttg tcggacttgg tcaacacgac aaccggctgc
     1501 acgccactgc cgccgaccag cagcaggtag cgctcgatcc ggcgcgggtt gaaatccgca
     1561 tccagcccgc aaaccacgaa cacggtgtcg atgttggcgg cgatcacctg ctgcttgtag
     1621 tgctcgccgg cggcgccgcg cttgatcgcg gagaaccgcg gcagcaacgc gacgatgcgc
     1681 agcgcgttcg gcgcctcgcc ttcgaccagc acccagtcgc cgaccgcggg ccgcgattcc
     1741 ggggtgatct cgcccctgcg gtagctgccg ccgcgctgcc attccggcaa cgactcggcg
     1801 cgatgcgagg catcgacgga ttcggcaacc aggtaaccgc tgcgatgctg ttcgctgatc
     1861 cgcgcctgcc gcgcctgcgg atgcgccgcc atcaattcgg cccaggcggg gtcgaggctg
     1921 gcctgcgtcc accgccagcc gatggcctgc aggggggtca tgcgcgttgg aatgcggtca
     1981 tgcggcgatt ctacccgtgg tcgcaagtgg cgttttgagc cgcggagcga accacccggc
     2041 ggtaggcgcc gttgctgcga gaaaccccgc ttgcgcggca tctggccgcg ctttccgcta
     2101 ggcttgcacc ctcgttttcc ctcactttcc gtgccaatgc ccccgcccac cctccagacc
     2161 cgcacccgcc tgtccgaagt ccgctacgaa atccgcggtg aactggcccg gcgggcccgc
     2221 gaactggagg cccagggccg caccctgatc aagctcaaca tcggcaaccc cggcgccttc
     2281 ggcttccgcg cgccggaaca cctgcagcgc gcgatcgccg accacatcgc ccgcaccgat
     2341 ccctacaccc accagcaggg cctgccggaa gcgcgcgagg cgatcgccgc cttccatcgc
     2401 cagcgcggca ccccgaacgc ctcgcccgag cgcgtgttca tcggcaacgg cgtcagcgag
     2461 ttgatcgaca tctccctgcg cgccctgctc aacccgggcg acgaagtcct gctgccctcg
     2521 cccgactatc cgctgtggtc cgccgcgacc atcctcaacg acggccgccc ggtctactac
     2581 cgctgcgacg aagccaacgg cttcctgccc gaccccgagc agatcgagtc gctggtatcg
     2641 ccgcgcaccc gcgccatcgt cctgatcaac ccgaacaacc cgaccggcgc gaactacccg
     2701 cgcgcgttgc tggagcgcat cgtcgccatc gccgcgaaac accgcctgct gttgatggcc
     2761 gacgagatct acgacggcat cctctacgac gaccacagct tccagccgct ggccccgctc
     2821 gccggcgacc tgccatgcct gagcttcggc ggcctgtcga aagtgcaccg cgcctgcggc
     2881 tggcgggtgg gctgggcggt gctcagcggc gacccgctgg ccagcggcga ctaccaccac
     2941 gcgatggacc tgctcggcgc gctgcgcctg tgcgccaacg tgccggggca gttcgcgatc
     3001 gagcaggccc tgcacggcgc cgacaccatc ggcgaactgg tgcgccccgg cggccgcctg
     3061 tacgaaaccc gcaaggcgct ggtcgaaagc tgcgccgcca gcgaacacct ctcgctggtc
     3121 gcgccggcgg gcgcgctgta tggcttcccg gcgatcgtcg gcgacgccgc gcatggcctc
     3181 gacgaccacg cgttcgcgct ggagatgctg gaaaccgaag acgtgctgat cgtgcccggc
     3241 tccagcttca acgtgcccta ccgcaaccac ttccgggtca ccctgctgcc ggaggcgccg
     3301 atgctgcgcg a
//
LOCUS       DAORUK010000194         3301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_39590_length_3301_cov_15.329945, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000194 DAORUK010000000
VERSION     DAORUK010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39590_length_3301_cov_15.329945"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PK818_10030"
     CDS             complement(<1..623)
                     /locus_tag="PK818_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83105.1"
                     /translation="MSMGDAAIAPAPTYRMGNALALLPLLIVLFSAWHYDLLANIRSD
                     YRWLVGTLFVGFAALAGGAHIAMQRGWRHAALLSLLAACVPLLGVVVYAGWASVGASL
                     LLIAAALALASCFHPASPSGIAIALMAGMACLMALVGWLLPFPIHHRAAYLIAALVLV
                     VARWRALREIGREASATLRGALSANPGWSILLIGATTIASLGLWLPSL"
     gene            complement(620..1876)
                     /gene="gmhB"
                     /locus_tag="PK818_10035"
     CDS             complement(620..1876)
                     /gene="gmhB"
                     /locus_tag="PK818_10035"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HQY83106.1"
                     /translation="MPADEAIVLAGGFGTRLRGIVDDVPKPLAPVAGRPFLAWLLDRL
                     AASGMRRCILATGYMSDAIERTVGAHWHGMEVAYSVEPEPLGTGGAIRLAVMQLRGTS
                     VHVLNGDTWLVYPPQALERATRESGALIGMALAEVADVGRYGAVERDGRGMVAGFREK
                     GEHGRGAINAGCYFLTEAALAALPAQAAFSFEREVLQPAAASGGVAGFLDTSGFIDIG
                     VPEDYQVAQGLFAAPDAHPVIRAPYIDPDAQALLASMPGPRRALFLDRDGVINVNHGY
                     VHTPAHTEWVAGIFDRVREARAAGYLPIVVTNQAGIGRGYYGEPAFLAYSAWMHERFA
                     EQGAPLLATYWCPHHAEAGLGDYRVDCTCRKPAPGMLLAAATAFDLDLPGSFLVGDKV
                     SDLEAGRAAGLTRVQLVADDGSWSLP"
     gene            complement(1866..2462)
                     /locus_tag="PK818_10040"
     CDS             complement(1866..2462)
                     /locus_tag="PK818_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HQY83107.1"
                     /translation="MSAMRDAIAAELDNAVRNIQALAADAAMQALLEQAVGLCTGALR
                     DGRKLLFAGNGGSAADAQHWAGELVSRFYYDRPGLAAIALTTDSSILTAIGNDYGYDY
                     TFARQVEALGQAGDVFIAISTSGNSPNILRAADAARARGIRVIGFTGASGGKLAPLAD
                     ICFCMPSSETPRIQEGHELIGHLLCALVEAEMYPRNAG"
     gene            complement(2459..>3301)
                     /locus_tag="PK818_10045"
     CDS             complement(2459..>3301)
                     /locus_tag="PK818_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HQY83108.1"
                     /translation="ASCLPGDLRADAGPLQLLAGTYLRISRDFLAGERPPLRIRSWSD
                     APPGSGLGSSSTLVVALVTALAEYCNLALGEYEVARLAYEIERVDLGLAGGRQDQYAA
                     AFGGFNFMEFHAQERVIVNPLRIKAWVLAELEASLLLYFTGVSRESARIIDEQARNAR
                     EGASVSLEAMHQLKAEAVQMKENLLQGDLAGFARTLQQGWQAKKRMASSISNPMIDSL
                     EALAMDNGARATKVSGAGGGGFMMFVCDPVDRMRLSAVLRGQGGSLLDFHFSPGGATA
                     WRVP"
BASE COUNT          563 a         1170 c         1118 g          450 t
ORIGIN      
        1 aggctcggca gccacaggcc gaggctggcg atggtggtag cgccgatcag caggatcgac
       61 cagcccggat tcgcggacag cgcgccgcgc agggttgcag acgcctcgcg cccaatctcc
      121 cgcaacgcac gccagcgcgc gacaaccagc accagcgccg caatgagata ggcggcacga
      181 tggtggatcg ggaacggcag cagccagccg accagcgcca tcaggcaggc catgcccgcc
      241 atcaatgcga tcgcgatacc ggacgggctt gccggatgga agcacgacgc caacgcgagt
      301 gccgccgcaa tgagcaagag gcttgcgccg accgaggccc aaccggcata taccacgacc
      361 cccaacaacg gcacgcaggc ggcgagcagc gacagcaacg ccgcgtgccg ccagccccgt
      421 tgcatggcga tgtgcgcacc accagcgagc gcggcgaaac cgacgaacag cgtgccgacc
      481 agccagcgat agtcgctgcg gatgttggcg agcaggtcgt aatgccacgc cgagaacagc
      541 acgatcagca gcggcagcag ggccaacgca ttccccatgc gatacgtcgg ggccggtgca
      601 atcgctgcgt cgcccatgct catggcagcg accagctccc gtcgtccgcg accagctgca
      661 cgcgggtcag gcccgccgcg cgtccggctt ccagatccga caccttgtcg ccaaccaaga
      721 acgaacccgg caagtccagg tcgaaggccg tggctgcggc gagcaacatg cccggcgcgg
      781 gcttgcggca agtgcagtcg actcggtagt cacccagccc ggcctccgca tggtgcgggc
      841 accagtaggt cgcgagcagc ggcgcgccct gttcagcgaa gcgttcatgc atccatgcgc
      901 tgtacgccag gaacgcgggt tcgccgtaat agccacgccc gatgcccgcc tggttggtga
      961 ccacgatggg caggtagccc gccgcgcgtg cttcccgcac gcgatcgaag atgcccgcca
     1021 cccactccgt gtgcgctgga gtgtgcacgt agccatggtt gacgttgatc acgccatcgc
     1081 ggtcgaggaa cagtgccctg cgcggtccgg gcatcgatgc cagcaacgcc tgggcatccg
     1141 ggtcgatgta gggcgcgcgg atcacgggat gcgcatcggg cgccgcgaac aacccctgcg
     1201 caacctggta gtcctccggc acgcctatgt cgatgaagcc ggacgtatcg aggaatcccg
     1261 cgaccccgcc cgacgcggcg gcaggttgca gcacttcgcg ttcgaacgag aacgccgcct
     1321 gcgccggcag cgccgccagc gcggcctcgg tgaggaaata gcagccggca ttgatcgccc
     1381 cgcggccgtg ttcgcccttc tcgcgaaagc ccgccaccat gccgcgcccg tcgcgttcga
     1441 ccgcgccgta acggccaaca tcagcgacct cggccagcgc cattccgatc agtgcaccgc
     1501 tctcgcgggt cgcgcgctcc agcgcctgcg gcggatacac cagccaggtg tcgccgttga
     1561 gcacgtgcac gctggtgccg cgcagctgca tgaccgcgag tctgatcgcg ccaccggtgc
     1621 cgaggggctc gggctcgacc gagtaagcga cttccatccc gtgccaatgg gccccgaccg
     1681 tgcgttcgat ggcatcggac atgtagccgg tcgcgaggat gcagcggcgc atgccggacg
     1741 ccgccaggcg atccagcagc catgccagga acgggcgtcc ggcgaccggc gccagcggct
     1801 tggggacgtc gtcgacgatg ccgcgcaggc gggtgccgaa gccgccggca aggacgatgg
     1861 cctcgtcagc cggcattgcg cgggtacatc tccgcttcga ccagcgcgca cagcaggtgg
     1921 ccgatcagct catggccttc ctggatgcgc ggggtctcgc tggagggcat gcagaagcag
     1981 atgtcggcca gcggcgcgag cttgccgccg gatgcgccgg tgaaaccgat cacgcggatg
     2041 ccgcgcgcgc gcgcggcgtc ggcggcgcgc aggatgttcg gcgaattgcc cgaggtggaa
     2101 atggcgatga acacgtcgcc ggcctggccg agcgcctcga cctggcgcgc gaaggtgtag
     2161 tcgtagccgt agtcgttgcc gatcgcggtg aggatgctgc tgtcggtagt cagcgcgatc
     2221 gcggccaggc ccggacggtc gtagtagaag cggctgacca gctcgccggc ccagtgctgc
     2281 gcatccgccg cgctgccgcc gttgccggcg aacagcagct tgcggccgtc gcgcaaggcg
     2341 ccggtgcaca ggcccaccgc ctgctcgagc agcgcctgca tcgcggcatc cgcggccagc
     2401 gcctggatgt tgcggacggc gttgtcgagc tccgcggcaa tcgcgtcgcg catcgcgctc
     2461 atggcaccct ccacgcggtc gcgccgccgg gactgaaatg gaaatcgagc aggctgccgc
     2521 cctgcccgcg cagtaccgcc gacagccgca tgcgatccac cgggtcgcag acgaacatca
     2581 tgaagccgcc accgcccgcg cccgagacct tggtcgcccg cgcgccattg tccatcgcca
     2641 gggcttccag cgaatcgatc atcgggttgc tgatgctgct ggccatgcgc ttcttcgcct
     2701 gccagccctg ctgcagggtg cgcgcgaaac cggccaggtc gccctgcagc aggttctcct
     2761 tcatctgcac cgcctcggcc ttgagctggt gcatggcctc cagcgagacc gacgcaccct
     2821 cgcgcgcgtt gcgcgcctgc tcgtcgatga tccgcgccga ctcgcgggag acaccggtga
     2881 agtacagcag cagcgaggcc tccagttcgg ccagcaccca cgccttgatc cgcagcggat
     2941 tgacgatcac ccgttcctgc gcgtggaatt ccatgaagtt gaagccgccg aacgccgccg
     3001 cgtactggtc ctggcggcca ccggccaggc cgaggtcgac gcgttcgatc tcgtaggcca
     3061 ggcgcgcgac ttcgtattcg ccgagcgcaa gattgcagta ctccgccagc gcggtgacca
     3121 gtgcgaccac cagggtggac gatgagccca acccggagcc gggtggcgca tccgaccagc
     3181 tgcggatgcg cagcggcggg cgttcgccag caaggaaatc ccggctgatc cgcaggtagg
     3241 taccggcgag cagttgcagc ggcccggcat cggcgcgcag gtcgcccggc aggcaggacg
     3301 c
//
LOCUS       DAORUK010000195         3239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_40835_length_3239_cov_18.489008, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000195 DAORUK010000000
VERSION     DAORUK010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40835_length_3239_cov_18.489008"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PK818_10050"
     CDS             complement(<1..784)
                     /locus_tag="PK818_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83109.1"
                     /translation="MNMNFPARVLAVLGGFSAIVMPSEGQAGNVGYYLNGSGSCTSSM
                     GDYAARITAAGHTPVAVSTLDAGSLAPLNGLVIAQGCNAYTGSAAVNTAVSNGLRVFI
                     EVQHVFAGDGAYLPGTPALAITSYHAGGTCYPDADLASGSPIATGPGGTLTDDSLDSS
                     VYCTPMGWASSSSLPAGSTPLLIANSNVNQVAAFSYAYGNGRVVYSQDQYFYFLSGGY
                     DPGTIVGSDAYVTNTIAWLMSTGPTTTCASEGYTGTKLQWCKY"
     gene            915..2639
                     /gene="gspE"
                     /locus_tag="PK818_10055"
     CDS             915..2639
                     /gene="gspE"
                     /locus_tag="PK818_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HQY83110.1"
                     /translation="MSAVLSDPVPASPDERIAALLLQRGKLKDADLARARRLQEETGG
                     GLLALLARLGLVSERDHAEACATELALPLRSARDVPELPPEDATLGQLPMSVKFMKQF
                     HVVPVAADAEGIDILLADPQDAYVLDAVRLACGREPRPAVALRSEIDELIERWHGQGR
                     SAMEGIVEHAEGEAGFDDVEHLRDLASEAPVIRLVNLVIQRAVELRASDIHIEPFENR
                     LKVRYRIDGVLIDGESPPQNLTAAVISRIKIMAKLNIAERRLPQDGRIMLRVQGKELD
                     LRVSTIPTAHGESVVMRLLDRETVVFDFERLGFTDAFLPQFQHVLQQPHGILLVTGPT
                     GSGKTTTLYTALSKLNTSDVKIITVEDPVEYQIEGINQIQAKPQIGLDFANALRSIVR
                     QDPDIIMIGEMRDLETARIAIQSALTGHLVLSTLHTNNAAGGITRLLDMGVEDYLLTS
                     TVNGILAQRLVRRLEPVHAERYPASPEEIEKFGLRRYQPQGDIYLYRPRGSDLAPTGY
                     LGRTTIMEFLVMDDELRRAVMRHAGMGELEQLARQAGMRTMYEDGIAKALAGLTTIEE
                     VLRVTEDA"
     gene            2639..>3239
                     /locus_tag="PK818_10060"
     CDS             2639..>3239
                     /locus_tag="PK818_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014504592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQY83111.1"
                     /translation="MPLYRYKALDARGQLLDGQMEAASDAEVAARLQEQGHLPVEARL
                     ASEAGGESAWRALFKPKPFAGQRLVQFTQQLATLLGAGQPLDRALTILLELPEDDVAR
                     RTLTDIRDAVRGGASLSAALDRQHGAFGRLYVNMVRAGEAGGSLHETLQRLADYLERS
                     RALRARVLNALVYPAILLAMVGLSLLFLLGYVVPQFSAMY"
BASE COUNT          547 a         1074 c         1098 g          520 t
ORIGIN      
        1 tgtacttgca ccattgcagc ttggtgccgg tatagccttc gctggcgcag gtggtagtgg
       61 ggcctgtgga catcaaccat gcgatcgtgt tggtgacgta cgcatcgctg ccgacgatgg
      121 tgccagggtc ataaccaccc gacaggaaat agaaatattg atcttgcgaa tagactaccc
      181 gtccattgcc ataggcgtag ctgaacgcag caacttggtt cacgttgcta tttgcaatca
      241 gcaacggtgt gctgccggcg ggcaaggagg aactgctggc ccagcccatt ggtgtgcaat
      301 agacagaact gtccagactg tcatctgtca atgttcctcc cgggccggtg gcaatgggag
      361 acccgctggc gaggtcggca tcggggtagc aagttccgcc cgcgtgataa cttgtgatcg
      421 cgagtgctgg agtcccgggg aggtatgcac catctccggc gaaaacatgt tgcacttcaa
      481 tgaatacacg cagcccattg gaaactgcgg tgttgacggc tgcgcttccc gtgtatgcat
      541 tgcaaccctg cgcaatgacc aagccattca gcggtgcgag cgagccagcg tccagcgtgc
      601 tgaccgctac cggggtgtgt cccgcagcag tgatgcgagc tgcatagtcg cccatgctgc
      661 ttgtgcaact cccgcttcca ttgaggtagt acccgacgtt acctgcttga ccctcggaag
      721 gcatcactat cgccgagaac cctcccagta ccgccaacac ccttgccgga aaattcatgt
      781 tcatgtcggt ctccgtcgcc gcaccacgcg gttggtcgtt actgtaaacg ggaaacgcgg
      841 ggcgtggcgg ccatcgcgcc cgcccggccg ccctgctaac ctcgcagcct gacttccgaa
      901 gccgtccgat gcccatgagc gctgtcctgt ccgacccggt cccggccagc cccgacgagc
      961 gcatcgcggc cctgttgctg cagcgcggca agctcaagga tgcggacctg gcccgcgcgc
     1021 gccgcctgca ggaggaaacc ggcggcggcc tgctggccct gctggcgcgg ctggggctgg
     1081 tgtccgaacg cgaccatgcc gaggcctgcg cgaccgagct ggcgctgccg ttgcgcagtg
     1141 cccgcgacgt gccggagctg cccccggaag acgccacgct gggccagctt ccgatgagcg
     1201 tgaagttcat gaagcagttc cacgtggtgc cggtggcggc cgatgccgag ggcatcgaca
     1261 tcctgctggc cgatccgcag gacgcctacg tcctggacgc ggtgcgcctg gcctgcggcc
     1321 gcgagccgcg gccggcggtg gcgctgcgca gcgagatcga cgagctgatc gagcgctggc
     1381 acggccaggg ccgcagcgcg atggaaggga tcgtcgagca tgccgagggc gaggccggct
     1441 tcgacgacgt cgagcacctg cgcgacctcg cctccgaggc gccggtgatc cggctggtga
     1501 acctggtgat ccagcgcgcg gtcgagttgc gcgcgtccga catccacatc gagccgttcg
     1561 agaaccggct gaaggtgcgc taccgcatcg atggcgtgct gatcgatggc gaaagcccgc
     1621 cgcagaacct gaccgccgcg gtgatcagcc ggatcaagat catggcgaag ttgaacatcg
     1681 ccgagcgccg gctgccgcag gacgggcgga tcatgctgcg ggtgcagggc aaggaactcg
     1741 acctgcgcgt gagcaccatc ccgaccgcgc acggcgaatc ggtggtgatg cgcctgctgg
     1801 accgcgagac ggtggtgttc gacttcgagc gcctgggctt caccgacgcc ttcctgccgc
     1861 agttccagca cgtgctgcag cagccgcacg ggatcctgct ggtgaccggg ccgaccggct
     1921 ccggcaagac caccaccctg tacaccgcgc tctccaagct caacaccagc gacgtcaaga
     1981 tcatcacggt cgaggatccg gtcgagtacc agatcgaggg catcaaccag atccaggcca
     2041 agccgcagat cgggctggac ttcgccaacg cgctgcgcag catcgtccgc caggatccgg
     2101 acatcatcat gatcggcgaa atgcgcgacc tcgagaccgc gcgcatcgcg atccagtcgg
     2161 cgctcaccgg ccaccttgtg ctgagcacgc tgcataccaa caacgccgcc ggcggcatca
     2221 cccgcctgct ggacatgggc gtggaggatt acctgctgac ctccacggtc aacggcatcc
     2281 tggcccagcg gctggtgcgc cggctggagc cggtgcacgc ggagcgctac ccggcatcgc
     2341 cggaggagat cgagaagttc ggcctgcgcc gctaccagcc gcagggggac atttacctgt
     2401 atcgcccgcg cggttccgat ctcgccccga ccggctacct cggccgcacc accatcatgg
     2461 aattcctggt gatggacgac gaactgcggc gcgcggtgat gcgccacgcc ggcatgggcg
     2521 aactcgaaca gctggcgcgg caggccggca tgcgcaccat gtacgaggac ggcatcgcca
     2581 aggcgctggc cgggctgacc accatcgagg aagtcctgcg cgtcaccgag gacgcctgat
     2641 gccgctgtac cgctacaagg cgctggatgc gcgcggccag ttgctggacg ggcagatgga
     2701 ggccgccagc gatgccgagg tcgcggcgcg cctgcaggag cagggccacc tgccggtgga
     2761 ggcccggctg gccagcgagg ccggcggcga gtccgcgtgg cgcgcgctgt tcaagccgaa
     2821 gccgttcgcc gggcagcggc tggtccagtt cacccagcaa ctggcgacct tgctcggcgc
     2881 cgggcagccg ctggaccggg cgctgacgat cctgctggaa ctgccggagg acgacgtcgc
     2941 ccggcgcacc ctgaccgaca tccgcgatgc ggtgcgcggc ggcgcgtcgc tgtcggcggc
     3001 gctggaccgc cagcacggtg cattcgggcg gctgtacgtg aacatggtcc gcgccggcga
     3061 ggcgggcggc agcctgcacg agaccctgca gcggctggcg gattacctgg aacgctcgcg
     3121 cgcgctgcgc gcgcgggtgc tcaacgcgct ggtgtacccg gcgatcctgc tggcgatggt
     3181 ggggctgtcg ctgctgttcc tgctcggcta cgtggtgccg cagttctcgg cgatgtacg
//
LOCUS       DAORUK010000196         3155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_42522_length_3155_cov_15.443871, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000196 DAORUK010000000
VERSION     DAORUK010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42522_length_3155_cov_15.443871"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PK818_10065"
     CDS             <1..766
                     /locus_tag="PK818_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83112.1"
                     /translation="KTTRWWAMLVAACAMALPMALAVAAEEPKPGPVANEWFMWVTPG
                     HEAQFEAAAKAHAAWRKQAGDPHTWNAYQATVGGDLTYYVYRAENLQWTDVDAGNAWD
                     QKAGATAHWNQNVNPHVARYEHYFGVMEPSMSYWLEAEDYAMYSVTTLPVNSGKWRSL
                     REGMQAYVKAATDAKWDRSWSIERVIGGDGGVIVVRPLRNFAEMADPPVMFRDMLIKQ
                     MGSEAAAQATMDKVVGGLGAGNTTIYTWRKDLSTPK"
     gene            852..1124
                     /locus_tag="PK818_10070"
     CDS             852..1124
                     /locus_tag="PK818_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83113.1"
                     /translation="MAAHLPLFSLRLGVGRTIGHQTLHTIWLRASRSADVTVSRTLLS
                     RQGADCYLYTFSGTRQIQDLAAVEGRLRQMIALEVSAARITLTRLS"
     gene            1183..1614
                     /locus_tag="PK818_10075"
     CDS             1183..1614
                     /locus_tag="PK818_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83114.1"
                     /translation="MQTQAHRERIGPVSGTPWEVRMKTLSMVFAVVLGLLAMAGGAQA
                     AVMRVVVVQTTDVAAYTKELAQVQAHMTRLGGTATIRAWRARFAGSDTGSIVVSVEYK
                     DMADFVAIDAKMRDDAANVATMKNMDAIRTIISDSLYDELK"
     gene            complement(1685..3142)
                     /locus_tag="PK818_10080"
     CDS             complement(1685..3142)
                     /locus_tag="PK818_10080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQY83115.1"
                     /translation="MASRPLNPLDASWLMVESRDTPMHVGGLLVFELPERAAPDYLQR
                     MMADFRASTRFAPPWNYRLRKPGLKGLRPSWVEQDTVDLEHHLRHAALPQPGGERELG
                     QLIARLHSQPLDLARPPWECALIEGLENRRFALYIKMHHSLIDGISGMKLLERAMAPD
                     AASSRRLRPFWACGASRRKAKATRETPPPTAAEAASALAEGLRAQLGTLPNVVSAFAG
                     MVEAWGKRSGTLRMPFDTPASILNGRITGPRRFATQQFPIERLKALAQAADATLNDVV
                     LALCGAALRRFLLELGELPEKPLTAGIPVSVRPKDDDTTGNAITFIISTLGTDIEDPR
                     ERMEAIKASTRRAKEHLQELPRQALMQYTIMLMAPYMLSLVTGVGGRTRPMFNVTISN
                     VPGPAMPLYFRGARLQATYPVSLVTHGQALNITCHGYAGTLAFGFTACRDTLPRMQRL
                     APYSAEALVELESAYAPKARRRAGGGAARRGARKP"
BASE COUNT          487 a         1087 c         1117 g          464 t
ORIGIN      
        1 caagacgaca cgctggtggg cgatgctggt ggcagcttgc gctatggccc tgccgatggc
       61 gttggcggta gccgccgagg agcccaagcc gggaccggtc gccaacgagt ggttcatgtg
      121 ggtaacgccg ggccacgagg cgcagttcga ggcggccgcg aaggcgcatg cggcgtggcg
      181 caagcaggcg ggcgacccgc acacctggaa cgcctaccag gccaccgtcg gcggggacct
      241 gacctactac gtctaccgcg ccgagaacct gcagtggacc gacgtcgacg ccggcaacgc
      301 ctgggaccag aaggccgggg ccaccgcgca ctggaaccag aacgtcaatc cgcacgtcgc
      361 gcgctacgag cactacttcg gcgtgatgga gccctcgatg agctactggc tcgaggccga
      421 ggactacgcc atgtactcgg tcaccaccct gcccgtgaac tcgggcaagt ggcgcagcct
      481 ccgcgaagga atgcaggcct acgtgaaggc tgcgaccgac gccaagtggg accgttcctg
      541 gtcgatcgag cgcgtgatcg gcggcgacgg cggcgtcatc gtcgtgcgac cgctgcgcaa
      601 cttcgccgaa atggcggacc cgccggtcat gttccgcgac atgctcatca agcagatggg
      661 ctccgaggcg gccgcccagg cgacgatgga caaggtggtc gggggcctcg gcgccggcaa
      721 taccaccatc tacacctggc gcaaggacct cagcacgccc aagtagtcac ccgggctgcc
      781 agcgggccgg cccggcaaac cgggccggcc cgttaggcgc ataatgacag cccgttcccg
      841 ctgcgaatcc catggctgcc cacctcccgc tgttctcgct gcgcctgggc gtggggcgga
      901 ccatcggcca ccagaccctg cacacgatct ggctgcgcgc atcgcgcagc gcggacgtga
      961 ccgtcagccg cacgctgttg tcgcgccagg gcgccgactg ctacctctac accttttccg
     1021 gcacgcggca gatccaggac ctggccgcgg tcgagggacg cctgcggcag atgatcgcgc
     1081 tcgaggtttc agccgcgcgc atcaccctga cgcggctgag ctgagccgcc caccggcccc
     1141 cggccggggt cgcgatcgca ggctccatgg cgccttgacc gcgtgcaaac gcaggcgcat
     1201 cgtgagcgga tcgggcccgt ttcgggcacg ccctgggagg ttcgcatgaa aactctgagc
     1261 atggtttttg ccgtggtact cggcttgctg gccatggccg gcggcgcaca agccgcggtg
     1321 atgcgcgtgg tcgtggtcca gaccaccgac gtggcggcct acaccaagga actggcccag
     1381 gtccaggcgc acatgacgcg cctcggcggc accgccacga tccgcgcctg gcgggcccgc
     1441 tttgccggca gcgataccgg atcgatcgtg gtgtcggtcg aatacaagga catggcggac
     1501 ttcgtcgcga tcgacgccaa gatgcgcgac gacgccgcca acgtcgccac catgaagaac
     1561 atggacgcga tccgcaccat catttccgac agtctctacg acgaactgaa gtagaacccg
     1621 gtccgcctga cccggcggcc cttgcgccgc cgggttgtgg ccaggccgcg gcgccccgcg
     1681 tcgactatgg cttgcgcgcg ccgcgacggg cagcgccgcc gcccgcgcgc cggcgcgctt
     1741 tcggcgcata agcggattcg agttcgacca gcgcttcggc gctgtagggc gccagccgct
     1801 gcatgcgcgg cagcgtgtcg cggcatgccg tgaagccgaa ggccagcgtg cccgcgtagc
     1861 catggcaggt gatgttgagc gcctgcccgt gcgtgaccag cgacaccgga taggtcgcct
     1921 gcaggcgcgc gccgcggaag tacagcggca tggccggccc gggcacgttc gagatcgtga
     1981 cgttgaacat cggccgcgtg cgcccgccga cgccggtcac caggctcagc atgtacggcg
     2041 ccatcagcat gatggtgtac tgcatcaagg cctggcgcgg cagttcctgc aggtgttcct
     2101 tggcgcgccg ggtcgaggcc ttgatcgcct ccatgcgctc gcgcgggtcc tcgatgtcgg
     2161 tgccgagcgt ggagatgatg aacgtgatcg cgttgccggt ggtgtcgtcg tccttgggcc
     2221 gcaccgacac cgggatcccg gcggtgaggg gcttctccgg caattcgccc agttcgagca
     2281 ggaagcgccg cagggccgcg ccgcacaggg cgaggaccac gtcattgagg gtcgcgtcgg
     2341 ccgcctgcgc caacgccttg aggcgctcga tcgggaattg ctgggtggcg aagcgccgcg
     2401 gaccggtgat gcgtccgttg aggatcgagg ccggcgtgtc gaacggcatc cgcagcgtgc
     2461 cggagcgctt gccccaggcc tcgaccatgc cggcgaaggc gctgaccacg ttgggcagcg
     2521 tcccgagttg ggcgcgcagg ccctcggcca gcgcgctggc ggcctccgcc gccgtcggtg
     2581 gcggcgtctc gcgggtcgcc ttggccttgc gccgcgacgc gccgcaggcc cagaacgggc
     2641 gcaggcgccg gctggaggcc gcgtcgggcg ccatcgcgcg ttcgagcagc ttcatgccgc
     2701 tgatgccatc gatcagcgag tggtgcatct tgatgtagag cgcgaagcgg cgattctcga
     2761 ggccctcgat cagcgcgcac tcccacggcg gccgcgcgag gtccagcggc tggctgtgca
     2821 ggcgcgcgat caactggccc agctcgcgct cgccgccggg ctgcggcagc gcggcatggc
     2881 gcaggtggtg ttcgaggtcc accgtgtcct gctcgaccca ggacgggcgc aggcccttga
     2941 gtcccggctt gcgcaggcgg tagttccagg gcggcgcgaa gcgcgtgctg gcgcggaagt
     3001 cggccatcat gcgctggagg taatccggcg ccgccctttc cggcagctcg aacaccagca
     3061 ggccgccgac gtgcatcggc gtgtcgcgcg actccaccat caaccacgag gcatcgagcg
     3121 ggttcagcgg tcggctggcc atggaccaga tcctt
//
LOCUS       DAORUK010000197         3152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_42583_length_3152_cov_19.574427, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000197 DAORUK010000000
VERSION     DAORUK010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42583_length_3152_cov_19.574427"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..269
                     /locus_tag="PK818_10085"
     CDS             66..269
                     /locus_tag="PK818_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HQY83116.1"
                     /translation="MSVAKIIELNASSASSIEDAVKHGLKKCAESVKHIKGAWVNEIK
                     VTTDDDGNVQEWRVNLRVSFIVN"
     gene            230..916
                     /gene="pdxH"
                     /locus_tag="PK818_10090"
     CDS             230..916
                     /gene="pdxH"
                     /locus_tag="PK818_10090"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010381128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HQY83117.1"
                     /translation="MAGQPAGQLHRQLTDGAPGGAPRPHARDNARMDNIPDDLRATFD
                     ALFAEAVTAGEPVPTAMTLATAVDGQPSARTVLLKAFDARGFVFYTHLDGRKGRELQA
                     NPRASLLFHWPRVREGVQVRIEGDVEIVAGDEADAYFASRPRGSQVGAWASLQSETLA
                     SRETFEDRIAQFEQEFEGREVPRPPRWGGFRLLPRHLEFWYGAQFRLHERWLHERGED
                     GTWSRRMLYP"
     gene            941..1759
                     /locus_tag="PK818_10095"
     CDS             941..1759
                     /locus_tag="PK818_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HQY83118.1"
                     /translation="MGPRRIVCLTEEPTEVLYALGEQDRIVGISGFTVRPPVARKQKP
                     KVSAFTSARIDEILKLRPDFVVGFSDIQADIAAALIRHGVEVWISNHRSVAGILDYIR
                     RLGALVGAGDKANAYADGLQRGLQAIERDAAALPSRPKVYFEEWDEPPITGIRWVAEL
                     VRIAGGDDIFPERALESLAKARILEDPDEVIRRAPDIILGSWCGKKFRPGRVAARPGW
                     HAIPAVRTGQLFEVKSPLILQPGPAALTDGVAEIARVIRAWAEQEGAAACRPGS"
     gene            complement(1756..2142)
                     /locus_tag="PK818_10100"
     CDS             complement(1756..2142)
                     /locus_tag="PK818_10100"
                     /inference="COORDINATES: protein motif:HMM:NF025822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4426 domain-containing protein"
                     /protein_id="HQY83119.1"
                     /translation="MPAASAAASTTVGGATLQASTVAIADLNATVAGRYGIDLAEEGV
                     LLLVTVRDSAGNGIDPGDLRLTATAGTLAEAPKPLDLRAISTDGMTDYIGVFRAVAPA
                     SVHFRLSATRSGARADIGTTAELQPR"
     gene            complement(2240..3058)
                     /gene="proC"
                     /locus_tag="PK818_10105"
     CDS             complement(2240..3058)
                     /gene="proC"
                     /locus_tag="PK818_10105"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HQY83120.1"
                     /translation="MNDSIAFIGGGNMARSLVGGLLARGRAPASIRIAEPVAALRDAL
                     QADFGVAVFADGAQAIEGAATWVLATKPQVLRGVCEALAAQAQAQRPLVVSIAAGITA
                     VQLERWLGGDIAVVRSMPNTPALLGAGVTGLYAGGKVDAAGRTFAEALLSAAGKTVWI
                     GDEALMDAVTAVSGSGPAYVFLLAEAMIDAGIAQGLPAEAARVLAIQTVLGAARMLDE
                     SGVDAAELRRRVTSPNGTTQAAVEHFEAGGLRTLVAGAIHAARIRGQELSAAND"
BASE COUNT          480 a         1096 c         1121 g          455 t
ORIGIN      
        1 ggaattaacg cccgaccggc aaggctgcgg gttcggcatc ccgccggcca accaggagtc
       61 ctgccatgtc cgtcgccaag atcatcgaac tcaatgcctc gtccgccagc agcatcgagg
      121 atgcggtcaa gcacgggctg aagaagtgcg ccgaatcggt caagcacatc aagggcgcct
      181 gggtcaacga aatcaaggtc accaccgacg acgacggcaa cgtgcaggaa tggcgggtca
      241 acctgcgggt cagcttcatc gtcaactgac tgatggtgcg ccgggtggtg cgccgcggcc
      301 gcatgcgcgc gacaatgcgc gcatggacaa catccccgac gacctccgcg ccaccttcga
      361 tgccctgttc gccgaggcgg tcaccgccgg cgaacccgtg cccaccgcga tgacccttgc
      421 caccgccgtc gacggccagc cgtcggcgcg cacggtgctg ctgaaagcct tcgatgcacg
      481 cggcttcgtg ttctacaccc acctggacgg gcgcaagggc cgcgaactgc aggccaatcc
      541 gcgcgcgtcg ctgctgttcc actggccgcg ggtgcgcgaa ggcgtgcagg tgcggatcga
      601 gggcgatgtc gagatcgtcg ccggcgacga ggcggatgcg tatttcgcaa gccggccacg
      661 cggcagccag gtcggggcgt gggcctcgct gcagtcggag acgctggctt cgcgggagac
      721 cttcgaagac cggatcgcgc agttcgagca ggaattcgaa ggccgcgagg tgccgcgccc
      781 gccgcgctgg ggcgggttcc ggctgctgcc gcggcatctc gagttctggt acggcgcgca
      841 gttccgcctg cacgagcgct ggctgcacga acgcggcgag gacggcacct ggtccaggcg
      901 gatgctgtat ccgtgagtgg gcctgccggt ggctgacgtg atcggcccgc ggcgcatcgt
      961 ctgcctgacc gaagaaccca ccgaagtcct ttacgcactg ggcgagcagg accgcatcgt
     1021 cggcatcagc gggttcaccg tgcgcccgcc ggtcgcgcgc aagcagaagc cgaaggtcag
     1081 cgcgttcacc agcgccagga tcgacgaaat cctcaagttg cgcccggact tcgtggtcgg
     1141 gttctccgac atccaggccg acatcgccgc cgcgctgatc cggcatggcg tggaggtctg
     1201 gatcagcaac caccgcagcg tggccgggat cctcgactac atccgccggc tgggtgccct
     1261 ggtcggtgcc ggcgacaagg ccaatgccta tgcggatggc ctgcaacgcg ggctgcaggc
     1321 gatcgaacgc gatgccgccg cgttgccatc caggccgaag gtctatttcg aggaatggga
     1381 cgaaccgccg atcaccggca tccgctgggt ggcggaactg gtccgcatcg ccggcggcga
     1441 cgacatcttc ccggagcggg cgctcgagtc gctggccaag gcgcgcatcc tggaagatcc
     1501 ggatgaagtg atccggcgtg cgccggacat catcctgggc tcatggtgcg gcaagaagtt
     1561 cagacccggg agggtcgccg cacgccccgg ctggcatgcg attccggcgg ttcgcaccgg
     1621 gcagctgttc gaggtcaagt cgcccttgat cctgcagcca ggcccggcgg cgttgacgga
     1681 cggcgttgcg gagatcgcac gggtcatccg cgcgtgggcg gagcaagagg gggccgcggc
     1741 ctgcaggcct gggtcctaac gcggttgcag ctccgccgtc gtcccgatat ccgcacgcgc
     1801 cccgctgcgc gtcgcggaga gcctgaagtg cacgctggcc ggcgccactg cgcggaacac
     1861 gccgatgtag tcggtcatgc cgtcggtgct gatcgcacgc aggtccagcg gctttggtgc
     1921 ttcggccagg gtgccggcgg tggcggtgag ccggaggtcg ccgggatcga tgccgttgcc
     1981 ggccgaatcg cgcacggtga ccagcagcaa cacgccttcc tccgcgaggt cgatgccgta
     2041 gcggccggcg acggtggcgt tgaggtcggc gattgcgacc gtcgaggcct gcagggtcgc
     2101 gccaccgacc gtcgtgctgg cggcggccga cgctgccggc atggcgaccg cgggtgtcgg
     2161 ggcctggtcg ccgctgcacg cgacgagcgc aagcagcagg acggcggcgg cggaacaagc
     2221 gatgcgcatg gcgggcccct cagtcgttgg cggcggacag ttcctgcccg cggatgcgtg
     2281 cggcatggat cgcgcccgcg accagcgtgc gcaacccgcc cgcttcgaaa tgttcgaccg
     2341 cggcctgggt ggtgccgttc ggcgaggtga cccggcgccg cagttcggcg gcatccaccc
     2401 cggattcatc gagcatgcgc gccgcgccga gcacggtctg gatcgccagc acgcgcgcgg
     2461 cctcggccgg caggccctgg gcgatgccgg catcgatcat cgcctcggcc agcaggaaca
     2521 cataggccgg tccactgccg gaaaccgcgg tcactgcgtc catcagggct tcgtccccga
     2581 tccacacggt cttgccggcg gcggacagca gcgcctcggc gaacgtgcgg cccgcggcat
     2641 cgaccttccc gcctgcgtac aacccggtca cgccggcgcc gagcagggcg ggcgtgttcg
     2701 gcatgctgcg cacgaccgcg atgtcgccgc ccagccagcg ctccagttgc acggcggtga
     2761 tgccggcggc gatcgacacc accagcggcc gctgcgcctg cgcctgcgcc gccagcgctt
     2821 cgcacacccc gcgcagcacc tgcggcttgg tcgccagcac ccaggtggcg gcgccctcga
     2881 tggcctgcgc gccgtctgcg aacaccgcaa ccccgaaatc cgcctgcagg gcgtcgcgca
     2941 gcgcggccac cggctcggcg atgcggatcg atgcaggcgc gcggccgcgc gcgagcaggc
     3001 cgccgaccag gctgcgcgcc atgttgccgc cgccgatgaa ggcaatgctg tcgttcatgc
     3061 gggtccttcc gttttcgcgg ggcgcgcgcc gaacagcgcg ctgccgatgc gcaccatggt
     3121 cgcaccttcc tcgatggcgg gcacgaagtc ct
//
LOCUS       DAORUK010000198         3128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_43143_length_3128_cov_20.625122, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000198 DAORUK010000000
VERSION     DAORUK010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43143_length_3128_cov_20.625122"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PK818_10110"
     CDS             complement(<1..365)
                     /locus_tag="PK818_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein"
                     /protein_id="HQY83121.1"
                     /translation="MNASSSRIARTLLLAALASAVAVGCSKKVKEAPPADTAGTATTT
                     TTPIDDGASTRPGAYGPADLDTDACLRNKVVYFDLDQDALKPEFQAAMACHAKYLRDR
                     PSARLRLEGNADERGSREY"
     gene            complement(452..1771)
                     /gene="tolB"
                     /locus_tag="PK818_10115"
     CDS             complement(452..1771)
                     /gene="tolB"
                     /locus_tag="PK818_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HQY83122.1"
                     /translation="MTRTGCRRSPFRHALAALLVLSPLQAFAQSRPLEIDIVGGNAAA
                     LPIAVVPFAGNSGQTDIDDIIRADLGRSGQFRTIDEAGLPERPTSAAEVAYPTWRMLK
                     QDFLLVGRSQPSGDGYRVEYELFDVARQERLLGFALTAPAGAMRDVAHQISDAVYEKI
                     LGVPGAFWTRIAYVTASGLGRNSRYALMVADADGHGPRNVVNSTEPLMSPSWSPDGRK
                     LAYVSFEGGNSGVYIQDIASGAREKVASFRGINGAPSFSPDGTRLALTLSKGGNPDIY
                     VMDLASKRLAQVTNHFGIDTEPSWTPDGASLVFTSDRGGRPQLYQVAASGGSATRISF
                     EGSYNASPSVSFDGKKIAVAQGAGNTYRIALLDRSLGGARWSMLSPGSLDESPSFAPN
                     GAMVLYAAREGRRGVLYAVSADARVRQRLVLSEGDVREPAWSPYRKR"
     gene            complement(1851..2735)
                     /locus_tag="PK818_10120"
     CDS             complement(1851..2735)
                     /locus_tag="PK818_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HQY83123.1"
                     /translation="MKETRADTRAALLQALGLHVVLFATMFAGLQWTRSNVAEAAQGE
                     VIEADLIDPTALGAAMRSALQRTPEALPQPVAEPEPLPPDPEPEPVPEPVPEALPPPV
                     PDPEPVEQEKVQRDAESPEVAKVPREQEEKRKPPVQADLDAQRVERVLAQIRKQREQA
                     ARERTQAEMRAQQLADARGAQPVAVSAPRGNPDSRSAVNALYSVALKEAITRQWTRPE
                     SIPLGVPCQVRIRQIPGGEVVDVQVSGGCPYDALGKRSLEAAVLKASPLPYAGFEDVF
                     SRDVILTFRPEDVDDFGR"
     gene            complement(2746..>3128)
                     /locus_tag="PK818_10125"
     CDS             complement(2746..>3128)
                     /locus_tag="PK818_10125"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HQY83124.1"
                     /translation="DVMLVLLIIFMVTAPLLNVGVDVNLPQADARPLEQKNTKPVVVQ
                     VASDGSLALTYEGLDRPQRVDRVQLVAKVKGFVDADPQLPVYVAGDRDASYQTVYEIL
                     TLLQREARVSRAGLMGNPADREGK"
BASE COUNT          428 a         1101 c         1111 g          488 t
ORIGIN      
        1 ttgtattcgc ggctgccgcg ctcgtcggca ttgccttcca ggcgcaggcg cgcggacgga
       61 cggtcgcgca ggtacttggc gtggcaggcc atcgccgcct ggaactcggg cttcagggcg
      121 tcctggtcga ggtcgaagta caccaccttg ttgcgcaggc aggcgtcggt gtcgaggtcg
      181 gccgggccgt aggcgccggg acgggtcgag gcgccatcgt cgatcggggt ggtggtcgtg
      241 gtggcggtgc cggcggtgtc ggccggcggg gcttccttga ccttcttgct gcaacccacg
      301 gccacggcgc tggccagggc ggccagcaac agggtgcggg cgatgcggga agaggatgcg
      361 ttcatcgggg ggtccttgtc ggcgcgggat gcggggatcg agtttagacc gggcgtgtcc
      421 ggcatgcgtg gacgccgccc ggccggttgg ttcatcgctt gcggtacggc gaccacgccg
      481 gctcgcgcac gtcgccttcc gacagcacca ggcgctggcg cacccgggca tcggccgaca
      541 ccgcgtagag cacgccgcgg cggccttcgc gcgccgcgta caggaccatc gccccgttcg
      601 gcgcgaagct cggcgattcg tccagcgagc ccggcgaaag catgctccag cgcgcgccgc
      661 ccaggctgcg gtcgagcagg gcgatccggt aggtgttgcc ggcgccctgc gccaccgcga
      721 tcttcttgcc gtcgaacgag accgaaggac tggcgttgta gctgccctcg aagctgatcc
      781 gggtggcgct gccgccggat gccgccacct ggtacagctg cgggcggccg ccgcggtcgg
      841 aggtgaatac caggctggcg ccgtccgggg tccagctggg ttcggtgtcg atgccgaaat
      901 ggttggtgac ctgcgcaagg cgcttgctgg ccaggtccat cacgtagatg tccgggttgc
      961 cgcccttgga cagggtcagc gccagccggg tgccatccgg cgaaaagctc ggcgcgccgt
     1021 tgatgccgcg gaaactggcg accttctcgc gcgcgccgct ggcgatgtcc tggatgtaca
     1081 ccccggaatt gccgccctcg aagctgacat aggccagctt gcggccatcc gggctccacg
     1141 acggcgacat cagcggctcg gtggaattca ccacgttgcg cggaccgtgg ccatcggcat
     1201 ccgccaccat cagcgcatac cgggagttgc ggccgaggcc gctggcggtg acataggcga
     1261 tgcgggtcca gaacgcgccg ggcacgccga ggatcttctc gtacaccgca tccgagatct
     1321 ggtgggccac gtcgcgcatg gcgccggccg gcgcggtcag cgcgaagccc agcaggcgct
     1381 cctgcctggc cacgtcgaac agttcgtact cgacccggta gccgtcgccg ctgggctggc
     1441 tgcggccgac cagcaggaag tcctgcttga gcatgcgcca ggtcggatag gcgacctccg
     1501 cggcggaggt cgggcgttcc ggcaggccgg cttcgtcgat cgtgcggaac tggccggaac
     1561 ggccgaggtc ggcgcggatg atgtcgtcga tgtcggtctg cccgctgttg ccggcgaacg
     1621 gcaccaccgc gatcggcagc gccgcggcat tgccgccgac gatgtcgatc tccagcgggc
     1681 gcgactgcgc gaacgcctgc agcggcgaca gcaccagcag cgccgccagc gcatggcgga
     1741 agggcgagcg gcggcatccg gttcgcgtca tgggcacggg cactccgtta ctggacctgg
     1801 gacagggtcg ccgatccggc gaccgcgggg atgaacacga gcggactgca tcagcgaccg
     1861 aagtcatcca cgtcctccgg gcggaaggtg aggatcacgt cgcggctgaa cacgtcctcg
     1921 aaaccggcat acggcaacgg cgaggccttc aggacggcgg cctccagcga acgcttgccc
     1981 agcgcgtcgt agggacaccc ccccgatacc tgcacgtcca ccacttcgcc gccggggatc
     2041 tggcggatcc gcacctggca cggcaccccg agcgggatcg attccggccg cgtccactgg
     2101 cgggtgatcg cctccttcag cgccacgctg tacagggcat tgaccgcgga acggctatcc
     2161 ggattgcccc gcggtgcgga caccgcgacc ggctgcgcgc cgcgcgcatc cgccagctgt
     2221 tgcgcccgca tctcggcctg ggtgcgttcg cgcgcggcct gctcgcgctg cttgcggatc
     2281 tgggccagca cccgctccac ccgctgcgcg tcaaggtcgg cctgcaccgg cggcttgcgc
     2341 ttctcctcct gctctcgcgg gaccttcgcc acctcgggcg actccgcatc acgctgcacc
     2401 ttttcctgct cgaccggctc cggatccggc accggcggcg gcaacgcctc cgggaccggc
     2461 tccggcacgg gctcgggctc cgggtccggc ggaagcggtt caggctcggc gaccggctgc
     2521 ggcagggctt cgggcgtgcg ctgcagggca ctgcgcatcg ccgcgcccag tgcagtcggg
     2581 tcgatcaggt cggcttcgat cacctcgccc tgcgcggcct cggccacgtt gctgcgggtc
     2641 cactgcaggc cagcgaacat cgtcgcgaac agcaccacgt gcaggcccag cgcctgcagc
     2701 aatgccgccc gcgtatcggc gcgcgtctct ttcatcgacg ccggctcact tgccctcgcg
     2761 gtccgccgga ttgcccatca ggccggcgcg gctgaccctg gcctcgcgct gcagcagggt
     2821 caggatctcg tagacggtct ggtagctggc gtcgcggtcg ccggccacgt acaccggcag
     2881 ttgcggatcg gcgtcgacga agcccttcac cttcgccacc agctgcacgc ggtccacccg
     2941 ctgcggccgg tccaggcctt cgtaggtcag cgccaggctg ccgtcgctgg cgacctgcac
     3001 caccaccggc ttggtgttct tctgttccag cggcctggcg tcggcctgcg gcaggttcac
     3061 gtccaccccg acgttgagca gcggcgcggt gaccatgaag atgatcagca gcaccagcat
     3121 cacgtcga
//
LOCUS       DAORUK010000199         3126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_43179_length_3126_cov_19.531749, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000199 DAORUK010000000
VERSION     DAORUK010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43179_length_3126_cov_19.531749"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="PK818_10130"
     CDS             <1..173
                     /locus_tag="PK818_10130"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410993.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HQY83125.1"
                     /translation="LLLVSDQPMIPEGVKTEASDARVSADFVQNHIKVGIEALRLIRR
                     HGKSVRHLRFDE"
     gene            235..771
                     /locus_tag="PK818_10135"
     CDS             235..771
                     /locus_tag="PK818_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HQY83126.1"
                     /translation="MTTPVAVTSFWRDAGMAKWFGGGDAFDAECRDRFLDAHHAAARR
                     EHAHWMDDAEGALALLILLDQIPRNVFRGSGHAFASDGLARQYATQALAAGFDAASEP
                     ELRMFFYLPFEHSEDMADQDRAVELFAALGNDNLLAYANAHRDVIARFGRFPHRNAAL
                     GRSNTPDEQAWLDAGGGF"
     gene            781..2178
                     /locus_tag="PK818_10140"
     CDS             781..2178
                     /locus_tag="PK818_10140"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005416155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HQY83127.1"
                     /translation="MECALLWFRNDLRLADNPALDGLLRAGLAPIPVYIHAPDEHGGW
                     APGAASDAWRLRSLRALDADLRARGSRLQVLHGPTHDVLAQLVAATGAKAVYWNRRYE
                     PAIEARDAAVKQALRADGIDARSHSGALLFEPWQVQSLAGDPYKVFTPFWRTALKQWR
                     LPALQAVPERLPAPPALPGVVAIDALGLAPRLPWDRGFWDEWVPGEAGAHAALDRFAT
                     DALDGYGGNRDIPSLRGTSRLSPHLHFGEIAPWRIAHRLQDALPASDREHYLRELGWR
                     EFGHHLLHHFPHTPTHNFNPRFDDFGWATPTDAQLDAWRHGRTGIPIVDAGMRELWAT
                     GWMHNRVRMIVGSFLTKHLRVHWAHGARWFWGTLVDADLANNTLGWQWVAGTGADAAP
                     YHRVFNPVLQAQKFDPQGAYIARWVPELAGLPAAQRHAPWTGVTPRGYPRPVVDLAAG
                     RAGALAALAGIRAPR"
     gene            complement(2182..3105)
                     /gene="grxD"
                     /locus_tag="PK818_10145"
     CDS             complement(2182..3105)
                     /gene="grxD"
                     /locus_tag="PK818_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HQY83128.1"
                     /translation="MSLDPAVRTRIETLLAANPVVLFMKGNPRAPQCGFSSKAVGALD
                     ATGAAYAHVDVLADPEIREGIKAYGEWPTIPQLYIGGELVGGSDIILQMAGSGELHAA
                     LGLPAPDRTPPQITITPAAAAMLKQAIADAGDDFALQIEIDNAFNARLQLAQRDESAI
                     TSESAGIRAQFDLASAQRARGMSIDWADDARGKGLVIDNPNAPAKVRALTPAQARDRV
                     RDGSLVLVDVRPLDERLLAEAPVAYRHVDHGVAELEALPKDTALAFLCRSGARSAEAA
                     EHFRRLGFRELYNVEGGINAWALLDPNLRAY"
BASE COUNT          430 a         1057 c         1158 g          481 t
ORIGIN      
        1 cgctgctgct ggtcagcgac cagccgatga tcccggaagg cgtcaagacc gaggcttccg
       61 acgcgcgcgt ctccgccgat ttcgtgcaga accacatcaa ggtcgggatc gaggcgctgc
      121 gcctgatccg ccgccatggc aagtcggtgc ggcacctgcg cttcgacgag tgatgcgcat
      181 gccgcgtcca cgccggtgcg ggcatgatcg aggtccaccg aagcggagcg cgcgatgacc
      241 acacccgttg ccgtgacgtc cttctggcgc gatgccggga tggccaagtg gttcggcggc
      301 ggcgatgcgt tcgacgccga atgccgcgat cgtttccttg atgcccacca cgccgccgcg
      361 cggcgcgagc atgcgcactg gatggacgat gccgagggcg cgctggcgct gctgatcctg
      421 ctcgaccaga tcccgcgcaa cgtgttccgc ggcagcgggc acgcgttcgc cagcgatggg
      481 cttgcgcgcc agtacgcgac gcaggccctc gcggccggct tcgatgccgc cagcgagccg
      541 gaactgcgga tgttcttcta cctgccgttc gagcattcgg aagacatggc cgaccaggat
      601 cgcgcggtcg agctgttcgc ggcgctgggc aacgacaacc tgctggccta cgcgaacgcg
      661 caccgcgacg tgatcgcgcg tttcggccgc ttcccgcatc ggaatgccgc acttggccgt
      721 agcaataccc ctgacgagca ggcgtggctg gacgccggcg gaggtttcta gggggttccg
      781 atggaatgcg cattgctgtg gttccgcaac gacctgcggc tggccgacaa cccggcgctg
      841 gacggcctgc tgcgcgcggg gctggcgccg atcccggtct acatccatgc accggacgaa
      901 catggcgggt gggcgccggg tgcggcctcc gacgcctggc gtttgcgttc gctacgcgca
      961 ctcgatgccg acctgcgcgc gcgcggctcg cggctgcagg tgctgcacgg acccacgcat
     1021 gacgtgctgg cgcagctggt ggccgcgacc ggtgcgaagg cggtgtactg gaaccggcgc
     1081 tacgaaccgg cgatcgaagc acgcgatgcg gcggtcaagc aggcgttgcg cgcggatggc
     1141 atcgatgcgc gcagccacag cggcgcgctg ctgttcgagc cgtggcaggt gcagagcctg
     1201 gccggcgatc cgtacaaggt gttcaccccg ttctggcgca ccgcgctgaa gcagtggcgg
     1261 ctgccggcat tgcaggcagt accggagcgg ctgccggcac caccggcatt gccgggcgtg
     1321 gtcgccatcg atgcgctcgg gctggcgccc aggctgccgt gggaccgcgg gttctgggac
     1381 gaatgggtgc ccggcgaggc cggtgcgcac gcggcgctgg atcgcttcgc gaccgatgcg
     1441 ctcgacggct acggcggcaa tcgcgacatc ccgtcgctgc gcggtacctc gcggctgtcg
     1501 ccgcacctgc atttcggcga aatcgcgcca tggcggatcg cgcatcggct gcaggatgcg
     1561 ctgcccgcga gcgaccgcga gcactacctg cgcgaactcg gctggcgcga attcggccat
     1621 cacctgctgc accacttccc gcatacgccg acgcacaact tcaacccgcg cttcgacgac
     1681 ttcggctggg ccacgcccac cgacgcccag ctcgatgcct ggcggcacgg ccgcaccggc
     1741 atcccgatcg tggacgcggg catgcgcgag ctgtgggcca ccggctggat gcacaaccgg
     1801 gtacggatga tcgtgggcag cttcctgacc aagcacctgc gcgtgcactg ggcgcatggc
     1861 gcgcgctggt tctggggcac gctggtggac gcggacctcg ccaacaacac gctgggctgg
     1921 cagtgggtgg ccggcaccgg cgccgacgcc gcgccctacc accgggtgtt caacccggtg
     1981 ctgcaggcgc agaagttcga tccgcagggc gcgtacatcg cccgttgggt accggagctg
     2041 gccggactgc cggcggcgca gcggcatgca ccgtggacgg gcgtgacccc gcgcggctac
     2101 ccgcgtcccg tggtcgacct ggcggcgggc cgggcaggcg cactggcggc gctggccggg
     2161 atccgcgcgc cacgctgagc ctcaataggc gcgcaggttc gggtccagca aggcccaggc
     2221 gttgatcccg ccttcgacgt tgtacagctc gcggaagccg agcctgcgga agtgctcggc
     2281 ggcctcggcg ctgcgtgcgc cgctgcggca caggaaggcc agcgcggtgt ccttcggcag
     2341 tgcttccagt tcggccacgc cgtggtcgac gtggcggtag gccaccggtg cttccgcgag
     2401 cagtcgctca tccagcgggc gcacgtcgac cagcaccagg ctgccgtccc gcacgcggtc
     2461 gcgcgcctgc gccggggtca gggcgcggac cttggccggc gcgttcgggt tgtcgatcac
     2521 caggcccttg ccgcgggcat catcggccca gtcgatcgac atcccgcgcg cgcgctgcgc
     2581 gctggcgagg tcgaactggg cgcggatccc ggcggattcg ctggtgatgg cggactcgtc
     2641 gcgctgcgcc agttgcaggc gcgcgttgaa ggcgttgtcg atctcgatct gcagggcgaa
     2701 atcgtcgccg gcatcggcaa tggcctgctt cagcatcgcg gcggcggcgg gggtgatggt
     2761 gatctgcggc ggggtgcggt ccggtgccgg caggccaagc gcggcatgca gttcgccgct
     2821 gccggccatc tgcaggatga tgtcggagcc gccgaccagt tcgccgccga tgtacagctg
     2881 cgggatcgtc ggccactcgc cgtaggcctt gatgccttcg cggatctccg ggtcggccag
     2941 cacgtcgacg tgggcgtagg cggcgccggt cgcgtccagc gcgccgactg ccttggagga
     3001 gaagccgcat tgcggcgcgc gcgggttgcc cttcatgaac aggacgacgg ggttggcggc
     3061 gagcagggtt tcgatgcggg tgcggacggc ggggtcgagg gacatgggga tgttccgggt
     3121 gcgggg
//
LOCUS       DAORUK010000200         3093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_43937_length_3093_cov_20.892034, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000200 DAORUK010000000
VERSION     DAORUK010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43937_length_3093_cov_20.892034"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..123)
                     /locus_tag="PK818_10150"
     tRNA            complement(47..123)
                     /locus_tag="PK818_10150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(87..89),aa:Arg,seq:ccg)
     gene            230..1147
                     /gene="ubiA"
                     /locus_tag="PK818_10155"
     CDS             230..1147
                     /gene="ubiA"
                     /locus_tag="PK818_10155"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HQY83129.1"
                     /translation="MAYERDFPPIPAPEPLWRERLRQYWTLVRGDRPIGWLLLLWPTW
                     WGLWLAAKGMPPIWPLVVFSLGVWLTRSAGCVINDYADRWLDPNVERTRQRPLATGAV
                     SGREALAVFAVLMLVAFALVLTLNRLTAYMSIIGVLLAASYPYLKRHTYLPQVYLGMA
                     FGWGIPMGFAALTGGVPPLAWLLYVANIFWATAYDTWYAMVDRDDDLRMGAKSTAILF
                     GEMDLVAQALLFACMFMALALVGREAQMGLPYWVGLALALVLVAYQFVIARGRSREAC
                     FRAFLHNHWVGMVVFAGIALDLRWPAASG"
     gene            complement(1149..1889)
                     /locus_tag="PK818_10160"
     CDS             complement(1149..1889)
                     /locus_tag="PK818_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HQY83130.1"
                     /translation="MAATVNQASPIEVDGDVAAPLPWLFALRCLVCGERGARGRDLCA
                     ACDAALPRQGPACRGCALPLPPTGACAHCLPADPPGSAPPLDAVHAVFDYAFPLDRLL
                     PRLKFHGDFAAGRVLAQAMAVHCSGLPRPGAIVPIPLHRARLRERGYDQALELATPLA
                     RALRLPLLANALLRRKTTTAQSRLDAAARQRNLRDAFVVAGNACLPAHVVLVDDVMTT
                     GATLHAAARALRAAGVSRVDAWVCARVA"
     gene            1969..2910
                     /gene="bioB"
                     /locus_tag="PK818_10165"
                     /pseudo
     CDS             1969..2910
                     /gene="bioB"
                     /locus_tag="PK818_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010368929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
BASE COUNT          453 a         1089 c         1092 g          459 t
ORIGIN      
        1 gcgctatgcg cgagggcagg gaatcacgac gaagcgccga cggagatggc gcgcccggag
       61 ggattcgaac ccccgaccaa tggcttcgga agccactact ctatccggct gagctacggg
      121 cgcgtgccgc gcgatggcga agatcgcgga cgcgcattct cgcatgattg aacagagatt
      181 tcgtctgttc gtagcgggcc cgccggcgcg ccgctgctag gctaggcgca tggcctacga
      241 acgcgatttc ccaccgattc cggcacccga accgctttgg cgcgaacgcc tgcgccagta
      301 ctggacgctg gtgcgcggcg atcgcccgat cggctggctg ctgctgctgt ggccgacctg
      361 gtgggggctg tggctggcag ccaaggggat gccgccgatc tggccgctgg tcgtgttctc
      421 gctgggggtc tggctgaccc gctccgccgg ctgcgtgatc aacgactacg ccgaccgctg
      481 gctggacccg aacgtcgaac ggaccaggca gcgcccgctc gccaccggcg ctgtgtccgg
      541 ccgcgaggcc ttggcggttt tcgcggtcct gatgctggtg gcgttcgcac tggtgctgac
      601 gctgaaccgg ctcacggcct acatgagcat catcggcgtc ttgctggccg ccagctatcc
      661 gtacctgaag cgccatacct acctgccgca ggtctacctc ggcatggcct tcggctgggg
      721 catcccgatg ggcttcgccg cgctgaccgg cggggtgcct ccgctggcgt ggctgctgta
      781 tgtcgccaac atcttctggg ccaccgccta cgacacctgg tacgcgatgg tcgaccgcga
      841 cgacgacctg cggatggggg cgaagtccac cgcgatcctg ttcggcgaaa tggacctggt
      901 ggcgcaggcg ctccttttcg cctgcatgtt catggcgctg gcgctggtcg ggcgcgaggc
      961 gcagatgggc ctgccgtact gggtcgggct cgcgctggcg ctggtgctgg tggcttacca
     1021 gttcgtgatc gcgcgcggac gatcgcgcga ggcctgtttc cgcgcgttcc tgcacaacca
     1081 ctgggtgggg atggtggtgt tcgccgggat cgcactggac ctgcggtggc cggctgcatc
     1141 cggctgagtc aggctacccg cgcgcatacc cacgcatcca cccgcgacac cccggcggca
     1201 cgcagcgccc gcgcggcggc gtgcagggtc gcgccggtgg tcatcacgtc atcgaccagc
     1261 accacgtgcg ccggcaagca ggcgttgccg gcaaccacga atgcatcgcg caggttgcgc
     1321 tggcgtgccg ccgcgtccag ccgggattgc gcggtggtgg tcttgcgtcg cagcagcgca
     1381 ttggcgagca gcggcaaccg cagcgcgcgc gccagcggcg tcgccagctc cagcgcctgg
     1441 tcgtagccgc gctcgcgcag gcgggcgcga tgcagcggga tcggcacgat ggcacccggc
     1501 cgcggcaagc ctgagcagtg cactgccatg gcttgcgcca gcacccggcc ggcggcgaaa
     1561 tcgccatgga atttcagccg tggcagcagc cggtccagcg ggaaggcgta atcgaagacc
     1621 gcatgcaccg cgtccagcgg cggtgcgctg ccgggtgggt cggccggcag gcagtgcgcg
     1681 cacgcgcccg tgggtggcag cggcagcgcg caaccccggc aggccgggcc ctgccggggc
     1741 agggcggcat cgcaggcggc gcacaggtcg cgaccgcggg ccccgcgttc gccgcagacc
     1801 aggcaacgca gggcgaacag ccaaggcagc ggggcggcga cgtcgccgtc aacttcgatg
     1861 gggcttgctt ggttgacggt ggcggccatg cattccagac tgcccgcttc attgcagcgc
     1921 gtcagtcggg aaacccccat gccgtccatc gcccaatccc ccagcagcat gttccccatc
     1981 cgccacgact ggtcgcgcgc cgacgtcgaa gccctgttcg aattgccgtt caccgagctg
     2041 ctgcaccgcg ccggcagcgc gcaccggctg cacttcgatc cggccgaagt gcaggtcagc
     2101 accctgctgt cggtcaagac cggcggctgc cccgaggatt gcggctattg cccgcaggcg
     2161 gcccgctacc acaccggcgt ggacgcgacc aagctgatga gcaccgaatc ggtggtggcg
     2221 agggccaggc aggcgaggga cgccggcgcc agccgcttct gcatgggggc ggcctggcgt
     2281 tcgccgaagg accgcgacgt ccccaaggtc gcggcgatga tccgcgaggt gaaggcgctc
     2341 ggcctggaaa cctgcgccac cctcggcatg ctcagcggca cccaggcgcg cgcgctgaag
     2401 gacgccggcc tcgactacta caaccacaac ctcgacaccg accccgagtt ctacggcgac
     2461 atcatccaca cccgcgagat gcaggatcgc ctcgacacgc tcacccatgt gcgcgacgcc
     2521 ggcatgaaga cctgctgcgg cggcatcgtc ggcatgggcg aatcgcgccg ccagcgcgcc
     2581 ggcctgctgc agaccctggc caacctgccg gcgcacccgg attcggtgcc gatcaaccgc
     2641 ctggtgcagg tggaaggcac gccgttgcac ggcaccagcg agctggaccc gttcgagttc
     2701 gtgcgcagca tcgcggtggc gcggatcctg atgccggcat cgatggtgcg cctgtccgcc
     2761 ggccgcgagg gcatgagcga cgagctgcag gcgctgtgct tcgccgccgg cgccaattcg
     2821 atcttcttcg gcgagaagct gctgaccacc ggcaacccgg ataccgaacg cgacctggcg
     2881 ctgttcgcgc gcctgggcct gcgcccgatg ctggtggccg aggtggcgga cgacaccctg
     2941 tccggatgca ccaccacggt ccacgccggc atcaccgaag cggcctgagc gatgccgcgc
     3001 gaggaccttg cggcgcgcgt gcaggccgcg cgcgccctgc gcgaggccgc gcatcgccgt
     3061 cgccatctgc gcacggtgac ccggcgcgcg ggc
//
LOCUS       DAORUK010000201         3045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_45068_length_3045_cov_18.080602, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000201 DAORUK010000000
VERSION     DAORUK010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45068_length_3045_cov_18.080602"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="PK818_10170"
     CDS             <1..141
                     /locus_tag="PK818_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQY83131.1"
                     /translation="GLPDSDTLRSHLYNLRKVIDKPFDNPLLHTVQSAGYRIADLSTH
                     PG"
     gene            161..1186
                     /locus_tag="PK818_10175"
     CDS             161..1186
                     /locus_tag="PK818_10175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQY83132.1"
                     /translation="MPATSRPIRIAFLLQVVIASLVVVLCILGLAAARERLGDPAATL
                     GAVAIAVLAIAGASWLAYRATRRALAPISWLLREVARWDPQRPDVRALSPELIPGQVC
                     GPARQLAEALHALGERVDAYIARERDFTRDASHELRTPLTVIRVATDLIEGDHGLSER
                     SRRSLARIRAANSGMESLMDALLVLARDQGVALETEDFAVREVVEAELERIRPLVEGK
                     GLQLHLQMDAEPELHAHPRVLGVMLGNLLGNAVRFTDNGRISVRLAADRVEVTDTGIG
                     MDAASLARAFEPFWRAGIEHPAGAGLGLSIAQRLGQRCGWPLQLSSTPGTGTRATIVF
                     GTCFAGG"
     gene            1269..2816
                     /locus_tag="PK818_10180"
     CDS             1269..2816
                     /locus_tag="PK818_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY83133.1"
                     /translation="MSQPPRRSPVALHKRATPRKIATALLVVSLLGAAWYFWGKQKSA
                     DAEGGYRTEAVQRGDIRVAISATGTLSAISTVTVGSQISGLVTDVLVDFNSPVRKDQV
                     LATIDPSTYQAQIEQGSAQIASARAQLAQAQASLRNAQLDYQRKSDLGRQQLIARSDL
                     DLSRAALDQAQAQVNSAQAQIRQQTASTQTTRVNLQRTVIRSPVDGVVLTRKIEPGQT
                     VAASFSAPELFTIAEDLSKMKIELAVDESDIGQVKVGQAVNFTADAFPDRQFKGVVDQ
                     VRLSATTASNVVTYPVVVTVDNADGTLLPGLTVNAEIEVSKRTGVLKVANAALRYKPA
                     ETGAAPAQAPQGARGAGSMAEDLQASAQALGLDERQQAALQQALDDMRQRQAAREATA
                     QNGQQGGSRLFGGGTGGPRMVVMGGGAGNSGMMAQVRQRMRERMQQQFAGFRATLDDA
                     QRAKWDAAVDGLLNAKRAPVYKLVDGKPDMAMVRIGASDGTTTEISGNIKEGDLLVAG
                     ERAAEAK"
     gene            2813..>3045
                     /locus_tag="PK818_10185"
     CDS             2813..>3045
                     /locus_tag="PK818_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY83134.1"
                     /translation="MSQRAGDATASAVTPVILTRALEKVYAAGSEAEVVALKGVDVRI
                     LPGEFVAIMGPSGSGKSTLMNLIGCLDTPTAGS"
BASE COUNT          472 a         1070 c         1091 g          412 t
ORIGIN      
        1 ggcctgccgg attcggacac cctgcgcagc cacctctaca acctgcgcaa ggtcatcgac
       61 aagccgttcg acaacccgct gctgcatacc gtgcagagcg ccggctaccg gatcgcggac
      121 ctctcgaccc acccgggttg agccgcgcag gacgcagccc gtgcccgcga ccagccgccc
      181 gatccggatc gcgttcctgc tgcaggtggt catcgccagc ctggtggtgg tgctgtgcat
      241 cctgggtctt gccgcggcca gggaacggct gggtgatccc gccgcgacgc tgggcgcggt
      301 cgcgatcgcg gtgctggcga tcgccggtgc ctcctggctg gcttaccgcg ccacccgccg
      361 cgcgctggcg ccgatcagct ggctgctgcg cgaggtcgcg cgctgggacc cgcagcgacc
      421 ggacgtgcgc gcgctgtcgc cggagctgat cccgggccag gtctgcgggc ccgcccgcca
      481 gctggccgag gcgttgcatg cgctgggcga acgggtcgac gcctacatcg cccgcgaacg
      541 cgatttcacc cgcgacgcga gccatgagtt gcgcacgccg ttgaccgtga tccgggtggc
      601 gaccgacctg atcgaaggcg accacggcct gtccgaacgc tcgcgccgat cgctggcgcg
      661 gatccgcgcc gccaactcgg ggatggaatc gctgatggac gcgttgctgg tgcttgcgcg
      721 ggaccagggc gtggcgctgg aaaccgagga cttcgcggtg cgagaggtgg tggaggcgga
      781 actggagcgg atccggccgc tggtggaggg caagggcctg caattgcacc tgcagatgga
      841 tgccgagccg gagctgcacg cccatccgcg cgtgctcggg gtgatgctgg gcaacctgct
      901 gggcaatgcg gtgcgcttca ccgacaacgg ccgcatcagc gtgcgcctgg ccgccgaccg
      961 ggtggaggtc accgacaccg ggatcggcat ggatgcggcg tcgctggcgc gcgcgttcga
     1021 gccgttctgg cgcgccggca tcgagcaccc ggcgggcgcg gggctcgggc tgtcgatcgc
     1081 gcagcggctg gggcagcgct gcggctggcc gctgcagctc tccagcacgc cgggcacggg
     1141 cacacgtgcg accatcgtgt tcgggacgtg cttcgccggg ggttgaacct ggtgccgaca
     1201 ccccccgacc tgtcaaccgc gcgcgcactg gcgcgttccc gcccctttgc cgaagacctt
     1261 gcccccgcat gagccagcca ccacgtcgtt cccccgttgc cctgcacaag cgcgcgaccc
     1321 cgcgcaagat cgccaccgca ttgctggtcg tgtccctgct tggagcggcc tggtatttct
     1381 ggggcaagca gaagtccgcc gacgccgagg gtggctaccg caccgaggcc gtgcagcgcg
     1441 gcgacatccg ggtggcgatc tccgccaccg gcaccctcag cgcgatctcc acggtgaccg
     1501 tcggcagcca gatctcgggg ctggtgaccg atgtgctggt cgacttcaat tccccggtcc
     1561 gcaaggacca ggtgctggcg acgatcgatc cctccaccta ccaggcgcag atcgaacagg
     1621 gctcggcgca gatcgccagt gcgcgtgcgc agttggcgca ggcccaggcc agcctgcgca
     1681 acgcccagct cgactaccag cgcaagagcg acctcgggcg gcagcagctg atcgcccgta
     1741 gcgacctcga cctgtcgcgc gccgcgctgg accaggcgca ggcgcaggtg aattccgcgc
     1801 aggcgcagat ccgccagcag actgcctcga cccagaccac ccgggtcaac ctgcagcgca
     1861 cggtgatccg ctcgccggtg gacggcgtgg tcctgacccg caagatcgag ccgggccaga
     1921 ccgtcgccgc aagcttctcg gcgccggagt tgttcaccat cgccgaagac ctctcgaaga
     1981 tgaagatcga gctggcggtc gacgaatccg acatcggcca ggtcaaggtc gggcaggcgg
     2041 tgaacttcac cgccgacgcc ttccccgacc gccagttcaa gggcgtggtc gaccaggtgc
     2101 ggctgtccgc gaccaccgcc agcaacgtgg tcacctaccc ggtggtggtc accgtggaca
     2161 acgccgacgg caccttgctg cccggcctga ccgtgaacgc ggagatcgaa gtcagcaagc
     2221 gcacgggcgt gctcaaggtc gccaatgcag cgctgcgcta caagccggcg gaaaccggcg
     2281 cggcgccggc gcaggcgccc cagggcgcgc gcggtgccgg cagcatggcc gaggacctgc
     2341 aggccagcgc gcaggcgctg ggcctcgacg agcgccagca ggcggcgctg cagcaggcac
     2401 tcgatgacat gcgccagcgc caggctgcgc gcgaggccac cgcgcagaac gggcagcagg
     2461 gtggcagccg cctgttcggc ggcggaaccg gcggcccgcg gatggtggtg atgggcggcg
     2521 gtgccggcaa tagcgggatg atggcgcagg tgcgccagcg catgcgcgag cgcatgcagc
     2581 agcagttcgc cggcttccgc gcgaccctcg acgacgccca gcgcgcgaag tgggatgccg
     2641 cggtcgatgg cctgctcaac gccaagcgcg cgccggtcta caagctggtg gacggcaagc
     2701 cggacatggc gatggtgcgg atcggcgcca gcgatggcac caccaccgag atctccggca
     2761 acatcaagga aggcgacctg ctggtcgccg gcgaacgcgc ggcggaggcc aagtgagcca
     2821 gcgtgctggc gacgccaccg cgtcggcggt cacgccggtg atcctgacca gggccctgga
     2881 aaaggtgtac gccgccggca gcgaggcgga agtggtcgcg ctgaagggtg tcgacgtgcg
     2941 catcctgccc ggcgagttcg tggcgatcat gggcccgtcc ggctccggca agtcgaccct
     3001 gatgaacctg atcggctgcc tcgacacccc caccgcaggc agcta
//
LOCUS       DAORUK010000202         3034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_45343_length_3034_cov_17.043974, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000202 DAORUK010000000
VERSION     DAORUK010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45343_length_3034_cov_17.043974"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..708)
                     /locus_tag="PK818_10190"
     CDS             complement(127..708)
                     /locus_tag="PK818_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83135.1"
                     /translation="MRTPRFLQPFVLAGLFAMGLGSAHAQTFSLDWNPRSGDVWVDAQ
                     LADMNQYGARYRDPFIDEMVRYHAAPRDLVTDLLVNRRWAPGDIYFACAIAQVIGRPC
                     RQVADEWERDHGQGWGVVAKRLGIKPGSAEFHRLKKGFVPSYDRWGRPIELDADLKRA
                     YPNHGQGGKPDKAQGNEKGNGNGKGNGKGKGKP"
     gene            complement(819..1247)
                     /locus_tag="PK818_10195"
     CDS             complement(819..1247)
                     /locus_tag="PK818_10195"
                     /inference="COORDINATES: protein motif:HMM:NF016087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HQY83136.1"
                     /translation="MHRSRKAVLAVVAVLLAACDMDAPGGRVAAAPAVSSTMEFEGGR
                     PCVDCLGIDAWLQLEHDGGRRRYRLVEHYRGAGNGRRFEEAGEWIADGDLLRLRNADG
                     GERVYARLAEGSLQARDSRGQPLPAAADDVMMPVNFATVR"
     gene            complement(1351..2079)
                     /locus_tag="PK818_10200"
     CDS             complement(1351..2079)
                     /locus_tag="PK818_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQY83137.1"
                     /translation="MDDTRGGIALVGFDADDTLWKSQDYFDDAQLQFERIVSAYVDLA
                     DAGERLYAVEKRNLALFGYGVKGMVLSMIEAAVEITGARISAADLHRIVELGKALLRH
                     PVELLPGVREAVGSVARDWPVVLITKGDLFHQEAKVRDSGLSDLFRRIEIVSEKDAGT
                     YARLFAEFGVAPSQFAMVGNSLRSDIAPVLELGGWGVHVPYHLTWAHEAEAEVRTGGE
                     RMRSIEGLDRLPPALRELAALARS"
     gene            complement(2105..2896)
                     /locus_tag="PK818_10205"
     CDS             complement(2105..2896)
                     /locus_tag="PK818_10205"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019337980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HQY83138.1"
                     /translation="MHRCPAITAIFLLAFAWVGVAEARTVYRCVRGETISLATAPEPG
                     SKCTAKTIDDDAAMLPNLWGDLGVFSGTLYEWRAPDGSVMYTTRRLPGAVPYLKFTVA
                     TPEASPAHEGLGALGPPRLDAHAKEFRAAAKAHRVDDAFLRAIAHAESGFDPNAVSPK
                     GAQGVMQLMPDVQREYGVVDPFASRQSIDGGARFLRVLLRRYKGDFVLAAAAYNAGMG
                     AVAKFGGVPPYAETRAYVGKVRALHARYRSAMGLKPLDPPLRAAQ"
BASE COUNT          437 a         1089 c         1039 g          469 t
ORIGIN      
        1 ttgcattcca gcacttcggg cgacgccggg gcatcgacct cgaagcgcac gcggcggcaa
       61 tggcagccgc cgcggtgggt cacgaatccg gatgcggcga gcgttgtcat ccgcgcattc
      121 ccgggatcac ggcttgccct tgcctttccc gttgcctttc ccgttgccgt ttcccttttc
      181 gttgccctgg gccttgtccg gcttgccgcc ctggccgtgg ttcgggtacg cgcgcttgag
      241 gtcggcgtcc agttcgatcg ggcggcccca gcggtcgtag ctcggcacga agcccttctt
      301 caggcgatgg aactcggccg aacccggctt gatgcccagg cgcttggcga ccacgcccca
      361 gccctggccg tggtcgcgtt cccactcgtc cgccacctgg cggcaggggc gaccgatgac
      421 ctgggcgatg gcgcaggcga agtagatgtc gcccggcgcc cagcggcgat tgaccagcag
      481 gtcggtgacg aggtcgcgcg gggctgcgtg gtagcgcacc atctcgtcga tgaaggggtc
      541 gcggtagcgg gcgccgtact ggttcatgtc ggccagctgg gcgtcgaccc agacgtcgcc
      601 gctgcgcgga ttccagtcca ggctgaaggt ctgcgcgtgc gcgctgccca ggcccatcgc
      661 gaacaggccg gcgaggacga acggttgcag gaaacggggc gtgcgcatcg ggtgctcctc
      721 ggcgcgggcg gtgcccgcag gggtgtcgcc aaactgcggc gagtgtcggc cgcggtggct
      781 gaccacgacg tgaaaaacgc gggcgccgaa ccatccgctc agcgcacggt tgcgaagttg
      841 accggcatca tcacgtcgtc cgcggccgcc ggcagcggct gcccgcgtga atcccgcgcc
      901 tgcagcgagc cctcggccag gcgcgcatac acgcgctcgc cgccgtcggc attgcgcagg
      961 cgcagcaggt cgccatccgc gatccactcg ccggcttcct cgaaacgacg cccgttgccg
     1021 gcacctcggt agtgctcgac caggcggtag cggcggcggc cgccgtcatg ctcaagttgc
     1081 agccacgcgt cgatgccgag gcaatccacg catggccggc cgccctcgaa ttccatcgtg
     1141 ctgctcaccg ccggcgcggc tgccacgcga ccgccaggcg cgtccatgtc gcacgccgcc
     1201 agcaacacgg cgacgaccgc aagtacggct ttccgggatc gatgcatgcc gccgattgtg
     1261 gcgcgcccgg ggtacctgcg ggcgcacgga cggcgccgca caggcaccgt tcgtcgcatt
     1321 gccctgcggc ggcccgggcc gcgttacgcc tcagctgcgc gccagggcgg ccagctcgcg
     1381 cagcgccggt ggcagccggt cgaggccctc gatgctgcgc atgcgctcgc cccccgtgcg
     1441 cacctcggct tcggcctcgt gcgcccaggt caggtggtag ggcacgtgca ccccccagcc
     1501 accgagttcc agcaccggcg cgatgtccga gcgcagcgaa ttgccgacca tcgcgaactg
     1561 cgatggggcg acgccgaact ccgcgaacag ccgcgcgtag gtccctgcgt ccttttcgct
     1621 gacgatctcg atccgccgga acaggtcgga caggccgctg tcgcgcacct tcgcttcctg
     1681 gtggaacagg tcgcccttgg tgatcagcac caccggccag tcgcgggcga cgcttcccac
     1741 cgcttcgcgc acgccgggca gcagctccac cggatgccgc agcagcgcct tgccgagctc
     1801 gacgatgcgg tgcaggtcgg cggcactgat gcgcgcgccg gtgatctcca ccgccgcctc
     1861 gatcatcgac agcaccatgc ccttcacgcc gtagccgaac agggccaggt tgcgcttctc
     1921 gaccgcatac aggcgttccc cggcatcggc gaggtcgacg taggcgctga cgatgcgctc
     1981 gaactgcagc tgcgcatcgt cgaagtaatc ctggctcttc cacagggtgt cgtcggcatc
     2041 gaaaccgacc agggcaatgc cgccgcgcgt gtcgtccatg cgcgaagtgt aagcgtcggg
     2101 cgcgctactg cgccgcgcgc agtggcgggt ccagtggctt gagccccatc gcgctgcggt
     2161 agcgggcgtg caacgcacgc accttgccga cataggcccg ggtctccgcg taaggcggca
     2221 ccccgccgaa cttcgcgact gcgcccatgc cggcgttata cgccgcggca gcgaggacga
     2281 agtcgccctt gtagcgccgc agcagcacgc gcaggaacct ggcgccgccg tcgatggatt
     2341 gcctggaggc gaacggatcg accacaccgt actcgcgctg gacatcgggc atcagctgca
     2401 tcacgccctg cgcacccttg ggcgagaccg cgttcggatc gaaaccgctt tccgcatgcg
     2461 cgatcgcgcg caggaaggca tcgtccaccc tgtgcgcctt cgccgccgca cggaactcct
     2521 tcgcgtgcgc atccagccgc ggcgggccaa gcgcgcccag gccttcgtgc gcgggcgagg
     2581 cttccggcgt ggccacggtg aacttcaggt agggcaccgc gcccggcagg cgccgggtgg
     2641 tgtacatcac gctgccatcg ggcgcgcgcc attcgtacag ggtgccgctg aacacgccga
     2701 ggtcgcccca caggttgggc aacatcgccg cgtcgtcgtc gatggtcttc gccgtgcact
     2761 tgctgccagg ctccggcgcg gtggccaggc tgatggtctc gccgcgcacg cagcggtaaa
     2821 ccgtgcgtgc ttccgccacg cccacccacg cgaacgcgag caggaagatc gcggtgatgg
     2881 cggggcagcg atgcatcggc gcgattgtag gcagtggcct gcgctacacc ggcagcgcgg
     2941 tggtcttctt caccgtgcgc aacgccagcg tggactgcac gcgcaccacg ccgggcagct
     3001 gggtgaggat ctcggtgtgg atgcgctcga agtc
//
LOCUS       DAORUK010000203         2948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_47448_length_2948_cov_18.810232, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000203 DAORUK010000000
VERSION     DAORUK010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47448_length_2948_cov_18.810232"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="PK818_10210"
     CDS             <1..857
                     /locus_tag="PK818_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQY83139.1"
                     /translation="VFHNVCRHRAGPIAQCDGLRAKSLRCRYHGWNYGLDGVLKSAPE
                     MGDAQDFDPAGVRLPQLAVQVWQGLVFAAVDPARAPAFADFVAGIDARLGPDRGLHRY
                     GGHHRASYDIACNWKVYVDNYLEGYHVPHVHPGLNRLLDYRSYRTELARWHSLQWSPL
                     ESDAALYGNGDALYYWLWPNAMLNILPGRLQTNRVIPLGVDHCRVLFDSYYALDAAGI
                     ADEAKRNADLAFSDEVQREDLGICEDVQRGFASGSYVPGRLNPLRETGVHHFHELLRT
                     AYRGRDGA"
     regulatory      1057..1188
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1288..>2948
                     /gene="gcvPA"
                     /locus_tag="PK818_10215"
     CDS             1288..>2948
                     /gene="gcvPA"
                     /locus_tag="PK818_10215"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HQY83140.1"
                     /translation="MSTLRSLEHHDAFIERHIGPNDGEIAAMLQVVGHDSLEALTDAI
                     VPASIKSAAPLALPDAVGEVEALARIRAIAAKNQVFRSFIGQGYYGTHVPNVILRNIL
                     ENPAWYTAYTPYQAEISQGRMEALINFQTMCADLTGMDIANASLLDEATAAAEAMTLA
                     RRSAKSKSGTFLVAGDTHPQTLALLQTRAEPLGITVAVVRSTEAFHQALAAGDYFGVL
                     VQYPASSGWIADWDQDAGVIHSHNALFVVATDLLALTLLKPPGEMGADIVIGNTQRFG
                     VPFGFGGPHAAFMACRDAYKRSMPGRLIGVSIDAEGKPAYRLTLQTREQHIRREKATS
                     NICTAQVLLAVMASMYAVYHGPEGLTRIASRVARMTAILAAGLRQLGHEAVHDTAFDT
                     LCIDAGDRGPAILERARAHRANLRVRGNGSLCISLDETTTRADLELLWTIFAGEGEAL
                     PSIDALDASAPSLIPQALRRTSRYLTHPVFNTHHSEHELLRYMRSLADKDLAMDRTMI
                     PLGSCTMKLNATAEMIPVTWPEFGDIHPLAPADQAAGYQDLIAGL"
BASE COUNT          475 a         1083 c          979 g          411 t
ORIGIN      
        1 gggtcttcca caacgtctgc cggcaccgcg ccgggccgat cgcgcaatgc gacggcttgc
       61 gcgcgaagtc gctgcgctgc cgctaccacg gctggaacta cggtctggac ggggtgctga
      121 agtcggcgcc ggagatgggc gacgcgcagg atttcgatcc cgccggggtc cgcctgccgc
      181 aattggcggt gcaggtctgg cagggcctgg tgttcgccgc ggtcgatccg gcacgggcgc
      241 ctgcattcgc cgatttcgtc gccggcatcg atgcccgcct cggcccggac cgcgggctgc
      301 accgctacgg cggccaccat cgcgccagct acgacatcgc ctgcaactgg aaggtctacg
      361 tcgacaacta cctggagggc taccacgtcc cgcacgtgca ccccgggctg aaccgcctgc
      421 tcgactaccg cagctaccgc accgaactgg cgcgctggca ttcgctgcag tggagcccgc
      481 tggagagcga cgccgcgctg tacggcaacg gcgacgcgct gtactactgg ctgtggccga
      541 acgcgatgct caacatcctg cccggccgcc tgcagaccaa ccgggtgatc ccgttggggg
      601 tggatcactg ccgcgtgctg ttcgacagct actacgcgct ggatgccgcc ggcatcgccg
      661 atgaggcgaa gcgcaacgca gacctggcct tcagcgacga ggtccagcgc gaggacctcg
      721 gcatctgcga ggacgtgcag cgcggcttcg cctccggcag ttacgtgccg ggccggctga
      781 acccgctgcg ggaaaccggg gtccaccact tccacgagct gctgcgcacg gcgtaccgcg
      841 ggcgcgacgg cgcctgaacc ccgcgtgccg ggcaaccgtg gccgcgcccg ttgcgggtgt
      901 ccgggccccg gggctgcctg cgtaaccacc gggcatcggc gcggggtggc ggggtataat
      961 tcccctgcac ggtgggagaa gcgcgcgggc ctttcggcca cgccgcgctg ccgaaggcgc
     1021 aaccgcccgg aatcgctcag gccgaagacc gccgtgtgga caaactctgg agagatcggc
     1081 acgcccaacc accgggccgc gccggcgccg aaggggcaag aagccgcgcg gatcccctcc
     1141 ccgcccgcgc gcttccaaac tctcaggcaa aaggacaggg atgggcgagc agggctttcg
     1201 agcagcgcac gctgcgagcc tgcgagcgcc cacgcgaatg caagcgcgtg ggccggaaca
     1261 tcccgcccac gccttccgga gcaccacatg tcgaccctcc gctcgctcga gcaccacgac
     1321 gccttcatcg aacgccacat cggcccgaac gatggcgaga tcgccgccat gctgcaggtg
     1381 gtcggccacg actcgctgga ggcgctgacc gacgccatcg tgccggcctc gatcaagtcg
     1441 gcggccccgc tggcgctgcc ggatgcggtc ggcgaagtcg aggcgctggc gcggatccgc
     1501 gcgatcgcgg cgaagaacca ggtgttccgc agcttcatcg gccagggcta ttacggcacc
     1561 cacgtgccga acgtgatcct gcgcaacatc ctcgagaacc cggcctggta caccgcctac
     1621 acgccgtacc aggcggagat ctcgcaaggc cggatggaag cgctgatcaa cttccagacg
     1681 atgtgcgccg acctcaccgg gatggacatc gccaatgcat cgctgctgga cgaagccacc
     1741 gccgccgccg aggcgatgac cctggcccgg cgctcggcga agtcgaagtc cggcaccttc
     1801 ctggtcgccg gcgacaccca tccgcagacc ctggcgctgc tgcagacgcg cgccgaaccg
     1861 cttggcatca cggtcgcggt cgtccgttcc accgaggcgt tccaccaggc gctggcggcc
     1921 ggcgattact tcggggtgct ggtgcagtac cccgcgtcga gcggctggat cgccgactgg
     1981 gaccaggacg ccggggtgat ccattcgcac aacgcgctgt tcgtggtcgc caccgacctg
     2041 cttgcgctga ccctgctgaa gccgccgggc gagatgggcg cggacatcgt gatcggcaat
     2101 acccagcgct tcggcgtgcc gttcggcttc ggcggcccgc acgccgcctt catggcctgc
     2161 cgcgatgcct acaagcgctc gatgccgggc cgcctgatcg gggtgtcgat cgacgccgaa
     2221 ggcaagccgg cctaccggct gaccctgcag acccgcgaac agcacatccg ccgcgagaag
     2281 gcgacctcca acatctgcac cgcgcaggtg ctgctggcgg tgatggcctc gatgtacgcg
     2341 gtctaccacg gccccgaagg cctcacccgg atcgcctcgc gggtggcgcg gatgaccgcg
     2401 atcctcgccg ccggcctgcg ccagctcggc cacgaagccg tgcacgacac cgcgttcgac
     2461 accctgtgca tcgatgccgg cgaccgcggc ccggcgatcc tcgaacgcgc cagggcccat
     2521 cgcgccaacc tgcgcgtgcg cggcaacggc tcgctgtgca tctcgctgga tgaaaccacc
     2581 acccgcgccg acctcgaact gctgtggacc atcttcgccg gcgaaggcga ggcgctgccg
     2641 tcgatcgacg cgctggatgc cagcgcgccg tcgctgatcc cgcaggcgct gcggcgcacc
     2701 agccggtacc tgacccatcc cgtgttcaac acccaccaca gcgaacacga gctgctgcgc
     2761 tacatgcgct cgctggcgga caaggacctg gcgatggatc gcaccatgat cccgttgggc
     2821 agctgcacca tgaagctcaa cgccaccgcg gagatgatcc cggtcacctg gcccgagttc
     2881 ggcgacatcc atccgctggc cccggccgac caggcggcgg gctaccagga cctgatcgcc
     2941 ggcctgga
//
LOCUS       DAORUK010000204         2945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_47529_length_2945_cov_17.307958, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000204 DAORUK010000000
VERSION     DAORUK010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47529_length_2945_cov_17.307958"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..986)
                     /locus_tag="PK818_10220"
     CDS             complement(276..986)
                     /locus_tag="PK818_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HQY83141.1"
                     /translation="MIDPGTWRQAAAGLPWLRTIDADRNARLHALAAEFLQRIAITPL
                     HGLALNEVQRLRLAMLCCLPLLEFGREGLHGWSQLLVYPDAFRVQRSHQDAHGVLHEW
                     DDELIGEAWERGPVILSWADVRADIATPGEGYCVAAHEIAHKLDALDGAMDGTPPLPR
                     DWQKRWASDFQRAYDALCTQVDAGRETAIDAYAAESPEEFFAVCSEYHFSDVATLRSA
                     MPEVAAHLQRFYGPPLLG"
     gene            complement(1026..1310)
                     /locus_tag="PK818_10225"
     CDS             complement(1026..1310)
                     /locus_tag="PK818_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83142.1"
                     /translation="MRLSPILLSLALLCASSQVLAAESREIEPDGNGNCPDAAPASND
                     GVDDPDGDAAATPVRRAQKAKAAPAARGGGSSSQRSSAPRWHSFLPGMIR"
     gene            1441..>2945
                     /locus_tag="PK818_10230"
     CDS             1441..>2945
                     /locus_tag="PK818_10230"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HQY83143.1"
                     /translation="MLEPAIPAPAIPVTEVTAAALRRAFIAGARRVIAARDELNRINV
                     FPVPDGDTGSNLSSTLGHVLQGALSRRSRHIGELLVRIGNDAIDGARGNSGAIMAQFL
                     YGLAEHARNAPALDARTLADAVRRGADSARAALAHPVEGTILSVISAFADALDEAARK
                     HGANPRGGFSSALQRARGALADTPKQMALLQKAGVVDAGAQGFVDWLEGIAEYVDGGP
                     RALRMRGAMAAANEPGAMPAHVHEDVDPARRYCTECLVLGEAIDRAAMQAALTALDID
                     SLVVAGGATRVRVHGHVGTPQRLFDACAGFGQVEAMKADDMVLQQRSAESPERVAVAT
                     DSAADLPAAVAERHGIHIVPVRVSLDGRDYLDRLGLTAGEFYRRMARSEKLPQTSQPP
                     AGDFRRLFEHVLAYQPAAVYVGLSRPLSGTLQAAETAARGFSRPLRVFDSGHASAGQA
                     LLAWRAGEMADAGADADAIAAELARLQPLTRTWAMARDISHAVRGGRIPRWA"
BASE COUNT          403 a         1045 c         1080 g          417 t
ORIGIN      
        1 cgcgtgcagg gccacggcat tggcctggcg atcgtgcagg acatcgtcag gagctatcgc
       61 ggcgagctgc aggtgacccg ctcgcaggaa ctcggcggcg ccaagttcga gatcgtgttg
      121 ccgccggggt tgtagggggc gcatgcgcgg cctgtgcctt ggggcaaggc ttcgccgctc
      181 tgtccagtca tccaggactg atgtcgcggg cgcgggccat ccctggcccg ctcgaagcct
      241 cgccccaagg cacaggccgc gcccggatcg tttcgctagc cgagcaaggg cgggccgtag
      301 aaccgttgca ggtgcgccgc gacttcaggc atcgcgctgc gcagcgtggc cacgtcgctg
      361 aagtggtatt cgctgcacac cgcgaagaat tcctcgggcg actccgcggc ataggcgtcg
      421 atggcggtct cgcggccggc atcgacctgc gtgcacagcg cgtcgtaggc gcgctggaaa
      481 tcggatgccc aacgcttctg ccagtcgcgt ggcagtggcg gggtaccgtc catcgcgcca
      541 tccagcgcgt cgagcttgtg cgcgatctcg tgcgcggcca cgcaatagcc ttcgcccggg
      601 gtggcgatgt cggcgcgcac gtcggcccac gacaggatca ccgggccgcg ttcccaggcc
      661 tcgccgatca gttcgtcgtc ccattcgtgc aacacaccat gcgcgtcctg gtggctgcgc
      721 tggacccgga aggcgtcggg atacaccagc agttgcgacc agccatgcag gccttcgcgg
      781 ccgaactcga gcagcggcag gcagcagagc atcgccagcc gcaggcgctg tacctcgttc
      841 agggcaaggc cgtgcagcgg cgtgatggcg atccgctgca ggaattcggc ggccagcgca
      901 tgcaggcggg cgttgcggtc ggcgtcgatc gtgcgcagcc acggcaaacc cgcggcagcc
      961 tgccgccatg tacctggatc gatcacgacc gccgccgcct tgcggcgcgg ccagaaccag
     1021 ctcacctagc ggatcatgcc cggcaggaag ctgtgccagc gcggcgcgct gctgcgctgg
     1081 ctgctgctgc caccaccgcg tgccgctggc gcggctttgg ccttctgggc gcggcgcacc
     1141 ggcgtggccg cggcgtcgcc gtcggggtcg tcgaccccgt cgttgcttgc gggggcagcg
     1201 tccgggcagt tgccattgcc atccggttcg atctcgcggg attccgcggc cagcacctgg
     1261 gaggatgcgc acagcagggc aaggctcagc aggatcgggc tcaggcgcat tcgactcacc
     1321 ggggattggc tggaggcgca cgcaaggcac ctttccacct cccactatag cctgactgcg
     1381 atggcacgcg atgctgcatc gcagcaggca ttgcggcgcg cgccgctgca gacttcgcgc
     1441 atgctcgagc ccgccatccc cgcgcctgcg atcccggtga cagaggtcac cgccgccgcc
     1501 ctgcgccgcg ccttcatcgc cggcgcccgc cgggtgatcg ccgcccgcga cgaactcaac
     1561 cgcatcaacg tgttcccggt gcccgacggc gacaccggca gcaacctgtc ctcgaccctg
     1621 gggcatgtgc tgcagggcgc gttgagccgg cgcagccggc acatcggcga gctgctggtg
     1681 cggatcggca acgacgccat cgacggtgcc cgcggcaact ccggcgcgat catggcgcag
     1741 ttcctgtatg gcctggccga acatgcgcgc aacgcgcccg cgctggatgc gcggaccctg
     1801 gccgatgccg tgcgccgcgg cgccgacagc gcgcgcgcgg cgctggcgca cccggtggaa
     1861 ggcaccatcc tcagcgtgat ctcggcgttc gcggacgccc tcgacgaggc cgcgcgcaag
     1921 cacggcgcca atccccgcgg cgggttctcg agcgcgctgc agcgcgcgcg cggtgcgctg
     1981 gcggataccc cgaagcagat ggcgttgctg cagaaggcgg gggtggtcga tgccggtgcg
     2041 caggggttcg tcgactggct ggaagggatc gccgaatacg tcgatggcgg cccgcgcgcg
     2101 ttgcgcatgc gcggcgcgat ggcggccgcg aacgagcccg gggcgatgcc ggcgcacgtg
     2161 cacgaagacg tggacccggc ccgccgctac tgcaccgaat gcctggtgct gggggaggcg
     2221 atcgaccgcg ccgccatgca ggcggcgctg accgcgctcg acatcgattc gctggtggtg
     2281 gccggcggtg ccacccgggt ccgcgtgcac ggccatgtcg gcaccccgca gcgcctgttc
     2341 gacgcctgcg cggggttcgg gcaggtggag gcgatgaagg ccgacgacat ggtgctgcag
     2401 cagcgcagcg ccgagtcgcc ggagcgggtc gcggtcgcca ccgacagcgc ggccgacctg
     2461 ccggcggcgg tggcggagcg ccacggcatc cacatcgtgc cggtgcgggt cagcctggac
     2521 gggcgcgatt acctggaccg gctcggcctc accgccggcg agttctaccg gcgcatggcg
     2581 cgcagcgaga aactgccgca gaccagccag ccgccggccg gcgatttccg ccgcctgttc
     2641 gagcacgtgc tggcctacca gccggcggcg gtgtatgtcg gcctgtcgcg gccgttgtcc
     2701 ggcaccctgc aggccgccga aaccgcggcg cggggcttca gccgcccgct gcgggtgttc
     2761 gacagcggcc acgcgagcgc cgggcaggcg ctgctggcgt ggcgcgccgg cgagatggcc
     2821 gatgccggcg ccgatgctga cgccatcgcc gccgaactgg cccgcctgca gccgctgacc
     2881 cggacctggg cgatggcgcg cgacatctcg catgcggtgc gcggcggccg catcccgcgc
     2941 tgggc
//
LOCUS       DAORUK010000205         2923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_48152_length_2923_cov_15.832985, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000205 DAORUK010000000
VERSION     DAORUK010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48152_length_2923_cov_15.832985"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PK818_10235"
     CDS             <1..735
                     /locus_tag="PK818_10235"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HQY83144.1"
                     /translation="DAREILEEPELYDPPPPGIVVERSHCPHCGHQLGWYENIPLLSW
                     LALRGKCRSCKAPISFQYPLVELLTALLFLACVWRFGFGWKGFGAMLLTSFLVAMSGI
                     DLRTRLLPDQLTLPLMWLGFVASIENLYVGQKSALLGAMAGYLSLWSVYWLFKQLTGK
                     EGMGHGDFKLLAALGAWTGLAGVLPTVLISSLVGAVVGSIWLLAQGKDKATPIPFGPY
                     LAIAGWISFFWGKDLMGWYMRIAGLA"
     gene            846..1463
                     /gene="coaE"
                     /locus_tag="PK818_10240"
     CDS             846..1463
                     /gene="coaE"
                     /locus_tag="PK818_10240"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466399.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HQY83145.1"
                     /translation="MSDYVIGLTGGIASGKSAVSQRFEALGIVVADADVAARDAVAIG
                     SEGLADVVDMFGTGVLAADGSLDRPAMRRRVFAEPDARKRLEGIIHPRVRELLRAACM
                     RAVGPYTIAAVPLLAEGGGRRAYPWLQRIAVVDVPESAQLQRLLQRDGIDEVLARRMI
                     AAQASRQQRLAVADDVLVNDGTLEALDGQVAALDRLYRNLAAASR"
     gene            complement(1520..2524)
                     /locus_tag="PK818_10245"
     CDS             complement(1520..2524)
                     /locus_tag="PK818_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005910877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HQY83146.1"
                     /translation="MPVAMNQIHVVAGVIRDARGRILLARRTECRDLAGLWEFPGGKV
                     EPGESPEAALVRELREELGIEARAGEALIRVPQQYPDKRLVLDVRRVDVRGVPKGLDG
                     QALAWVPPAKLASYPMPPADRPVVAALTQPDRYLVTPAPGDDDSAWMAGLAAALTHGV
                     DRVQLRAPGLDAARWERLAGHAARLCREAGAEVLVNGNAGLAERLGAGLHLRAAQLQQ
                     FVGAAHGRDAPGAEHRGHGPLLPGQEPRPLAASCHDIDDLLRAQALGCDFVVLGPVLP
                     TASHPGAPGIGWDAFAALRERVSLPIYAIGGLDPGDIGIARRHGGQGIAAIRGLWGLL
                     "
     gene            complement(2556..>2923)
                     /locus_tag="PK818_10250"
     CDS             complement(2556..>2923)
                     /locus_tag="PK818_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017112773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HQY83147.1"
                     /translation="RGYAQKQPKQEYKKEAFELFSELLEKVKREVVSLLARVRIRDEA
                     EIAAAEAEERARAEAIARQMQFQHPDMGGMGADEEAAAVQLAQQPQGYGEAFSRVGRN
                     DPCPCGSGKKFKHCHGQLA"
BASE COUNT          423 a         1013 c         1029 g          458 t
ORIGIN      
        1 gatgcgcgcg agatcctcga agaacccgaa ctgtacgacc cgccgccgcc cgggatcgtg
       61 gtggagcgct cgcattgtcc gcattgcggc caccagttgg gctggtacga gaacatcccg
      121 ctgctcagct ggctggcgct gcgcggcaag tgccgcagct gcaaggcccc gatctcgttc
      181 cagtacccgc tggtcgaact gctgaccgcg ctgctgttcc tggcgtgcgt gtggcggttc
      241 ggcttcggct ggaagggctt tggtgcgatg ttgctcacca gcttcctggt ggcgatgtcg
      301 ggcatcgacc tgcgcacccg gctgctgccc gaccagctca ccctgccgtt gatgtggctg
      361 gggttcgtgg cctcgatcga gaacctctac gtcggccaga agtccgccct gctgggcgcg
      421 atggccggct acctgagcct gtggtcggtg tactggctgt tcaagcagct gaccggcaag
      481 gagggcatgg gccacggcga cttcaagctg ctggcggcgc tgggcgcctg gaccggcctg
      541 gccggggtgt tgccgacggt gctgatctcg tcgctggtcg gcgcggtggt cggctcgatc
      601 tggctgctgg cgcagggcaa ggacaaggcc accccgatcc cgttcggccc gtacctggcc
      661 atcgccggct ggatcagctt cttctggggc aaggacctga tgggctggta catgcggatt
      721 gcggggctgg cctgacgtag gagcggaaaa ggggccagag tgaattttgc gccgacaaga
      781 tgcgatctga gcgagatgcc tagccgcaaa acttcactct ggcccctttt cccgagcccg
      841 cgtccatgag cgactacgtc atcggcctca ccggcggcat cgcctccggc aagagcgcgg
      901 tcagccagcg cttcgaggcg ctgggcatcg tggtggccga tgccgacgtg gcggcccgtg
      961 acgcggtggc gatcggcagc gagggcctgg ccgacgtggt cgacatgttc ggcacaggcg
     1021 tgctggccgc ggacggcagc ctggatcgcc cggcgatgcg ccggcgcgtg ttcgccgagc
     1081 cggacgcgcg caagcggctg gaggggatca tccacccgcg cgtgcgcgaa ttgctgcgcg
     1141 ccgcctgcat gcgcgcggtt ggaccgtaca cgatcgccgc ggtgccgctg ttggccgagg
     1201 gcggtggccg acgtgcctat ccgtggctgc agcggatcgc ggtggtcgac gtgccggaat
     1261 ccgcgcaact gcagcggttg ctgcaacgcg acggcatcga cgaggtcctg gcgcggcgaa
     1321 tgatcgcggc gcaggccagc cgccagcaac ggctggcagt cgccgacgac gtgctggtca
     1381 acgacggcac gctggaggcg ctggacggac aggtcgcggc gctggatcgg ctgtaccgga
     1441 accttgctgc ggcgtcccgg taggcgcggc ccatggccgc gatgcttcca ggccggcgca
     1501 tcgcggccat gggccgcgcc tacaagaggc cccacaatcc ccgaatcgca gcgatgccct
     1561 gcccgccgtg cctacgcgcg atgccgatgt ccccgggatc cagcccgcca atcgcataga
     1621 tcggcagcga gacccgctcg cggagcgcgg cgaaggcatc ccagccgata cctggcgccc
     1681 cgggatgcga ggcggtgggc aggaccggac ccagcacgac gaagtcgcag cccagtgcct
     1741 gcgcacgcag caggtcgtcg atgtcgtggc aggaagcagc gagcgggcgt ggttcctgtc
     1801 ccggcaggag cggcccatgg ccgcgatgct ccgcgccagg agcatcgcgg ccatgggccg
     1861 ctcctacaaa ctgctgcagt tgggcagcac gcaggtgaag ccccgcgcca agccgttcgg
     1921 ccaatccggc attgccattg accaacacct cggcccctgc ctcgcggcaa aggcgtgcgg
     1981 cgtgtccggc cagccgctcc cagcgcgctg catccaggcc aggtgcgcgc aactgcacgc
     2041 ggtcaacgcc atgcgtcaat gcggccgcca gccccgccat ccacgcgctg tcgtcatcgc
     2101 cgggcgcggg agtgaccagg tagcggtccg gctgggtcag cgcggccacc accggccggt
     2161 ccgcaggcgg catcgggtag ctggccagct tggcaggcgg tacccaggcc agcgcctggc
     2221 catccagccc cttcgggacg ccacggacgt cgacgcggcg cacgtccagc accagccgct
     2281 tgtccggata ctgttgcggc acgcggatca gcgcctcgcc ggcgcgggcc tcgatgccga
     2341 gctcctcgcg aagttcgcgg accagtgcgg cctcgggcga ttcgcccggc tcgaccttgc
     2401 cgcccgggaa ttcccacagg ccggcaaggt cgcggcactc ggtgcgccgc gccagcagga
     2461 tgcggccacg ggcatcgcga atgacgccgg cgacgacgtg aatctggttc atcgccacgg
     2521 gcaggaaggg tacggcctgg aaaaggggca gcctgctaag caagctgccc atggcagtgc
     2581 ttgaacttct tgccgctgcc gcacgggcac gggtcgttgc ggccgacccg gctgaacgcc
     2641 tcgccgtagc cctgcggctg ctgcgccagt tgcaccgcgg cggcctcttc gtccgcaccc
     2701 atcccgccca tgtcgggatg ctggaactgc atctgccggg cgatggcttc cgcgcgcgcg
     2761 cgctcctctg cctcggcggc ggcgatctcg gcctcgtcgc ggatgcgcac ccgcgccagc
     2821 agcgagacca cctcgcgctt gaccttctcc agcagctcgg agaacagttc gaacgcttcc
     2881 ttcttgtatt cctgcttcgg ctgcttctgc gcatagccgc gca
//
LOCUS       DAORUK010000206         2917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_48315_length_2917_cov_19.491614, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000206 DAORUK010000000
VERSION     DAORUK010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48315_length_2917_cov_19.491614"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PK818_10255"
     CDS             complement(<1..628)
                     /locus_tag="PK818_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY83148.1"
                     /translation="MFKPVPVAIGLRYLRAKRRNGFISFISLASILGIALGVTALITT
                     LSVMSGFQREIRDRMLQMAAHTTVSGYGEPLQDWQRAVQVALQDPRVAGAAPYVEKEA
                     LILGSNNQPAMLRGVEPAQEGKVSVIGDKLVQGKLVDLQPGGFRIVLGRELAVWLGVG
                     VGDDVIVMLADYRNTPMGGIQTQKRFVVSGIFEAGYNEFDKGLAVVNLQ"
     gene            complement(651..1025)
                     /locus_tag="PK818_10260"
     CDS             complement(651..1025)
                     /locus_tag="PK818_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009593469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83149.1"
                     /translation="MSWALALLAPFSLLASGLPRVAAWPLALAAGAWGVVSARRQRAM
                     PPRLLLVPPGRAAATCDGAPIVDLRFGWRGPLAFACWRDGTGRSHRASWWPDTLDAGM
                     RRELKVVMLRRDPAAEPASMAG"
     gene            complement(1054..1251)
                     /locus_tag="PK818_10265"
     CDS             complement(1054..1251)
                     /locus_tag="PK818_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HQY83150.1"
                     /translation="MRELDQLLERWLDREWRQSPTAQREVFLRLLDCEDDRLWRWFLG
                     HEVCPDVEIAALVDRIRSLPP"
     gene            complement(1302..2093)
                     /locus_tag="PK818_10270"
     CDS             complement(1302..2093)
                     /locus_tag="PK818_10270"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HQY83151.1"
                     /translation="MAEFTLPKNSQVKKGKHFPAASGAKQVRTFKVYRWNPDDGMNPR
                     VDTYDVDLASCGPMVLDALIKIKNEVDPTLTFRRSCREGICGSCAMNIDGTNTLACTK
                     AIGDCGKGNAEVPIYPLPHMPVVKDLVPDLTHFYAQYASIKPWLRTQTPAPPDRERLQ
                     SKADRAKLDGLYECILCACCSTSCPSYWWNGERYLGPAVLLQAYRWIIDSRDEDTGAR
                     LDDLEDPFKLYRCHTIMNCARTCPKGLNPAKAIAEIKQLMLARRA"
     gene            complement(2181..>2917)
                     /locus_tag="PK818_10275"
     CDS             complement(2181..>2917)
                     /locus_tag="PK818_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQY83152.1"
                     /translation="IPTVHYNMGGIPTNYHGEVVQPCNGNPDAVVPGLYAIGEAACVS
                     VHGANRLGSNSLLDLVVFGRAVANRCAETITPGAPHKQLAGDACDKALANLDRLRNAN
                     GGTSTAAIRNNMQRAMQNDAAVFRTGETLANGVRAIREINASFADVKVSDRSLVWNSD
                     LVETLELQNLLGQALATIVSAENRKESRGAHAREDFPERDDANWHKHSVCWVDDAGDT
                     RIDYRPVHMYTLSNDVDVVPPKPRVY"
BASE COUNT          439 a          981 c         1002 g          495 t
ORIGIN      
        1 cctgcaggtt gaccaccgcc aggcccttgt cgaattcgtt gtagccggcc tcgaagatgc
       61 cgctgaccac gaaccgcttc tgggtctgga tgccgcccat cggggtgttg cggtagtcgg
      121 ccagcatcac gatcacgtcg tcgccgacgc caacccccag ccacaccgcc agttcgcggc
      181 ccagcacgat ccggaaacca ccgggctgca ggtcgaccag cttgccctgc accagcttgt
      241 cgccgatcac cgagaccttg ccttcctgcg ccggctcgac cccgcgcagc atcgccggct
      301 ggttgttgct gcccaggatc agcgcttcct tctcgacgta tggcgcggcg ccggccaccc
      361 gcggatcctg caatgccacc tggaccgcac gctgccagtc ctgcagcggc tcgccgtagc
      421 cgctgacggt ggtatgcgcg gccatctgca gcatgcggtc gcggatctcg cgctggaatc
      481 cgctcatcac cgacagggtg gtgatcagcg cggtcacccc cagcgcgatg ccgaggatcg
      541 aggccagcga gatgaaggaa atgaagccgt tgcggcgttt cgcgcgcagg tagcgcaggc
      601 cgatggcgac ggggacgggt ttgaacatcg agggcccttc gagtgaccgc ctatcctgcc
      661 atcgatgcgg gttccgctgc agggtcccgg cgcagcatca cgaccttgag ttcacgccgc
      721 attccggcat ccagggtatc gggccaccac gatgcgcgat ggctgcggcc ggtgccatcg
      781 cgccagcatg cgaacgccag cgggccgcgc cagccaaacc gcaggtcgac gatcggcgcg
      841 ccgtcgcagg tggcggcggc gcgacccggc gggaccagca gcagccgcgg cggcatcgcc
      901 cgctggcggc gcgcggagac gaccccccag gcccccgccg cgagcgcgag cggccatgcc
      961 gccacgcgtg gcagtccgga ggctagcagg gagaacggcg ccagcagcgc cagcgcccac
     1021 gacatcgcgc ccagccagcg cgacgggcgc cactcaaggc ggcagggaac ggatgcgatc
     1081 gacgagcgcc gcgatttcga catcagggca aacctcatgg ccgaggaacc agcgccagag
     1141 cctatcgtcc tcgcagtcca gcaggcgcag gaaaacctcg cgctgcgcgg tgggactttg
     1201 ccgccattcg cgatccagcc agcgttccag caactggtcg agctcgcgca tgccgcgccg
     1261 gcaacgccag cgcagcttgc ggatttcggt ttcgtcggcg ctcacgcgcg gcgggcgagc
     1321 atcagttgct tgatctcggc gatcgccttg gccgggttca ggcccttcgg gcaggttcgc
     1381 gcgcaattca tgatggtgtg gcagcggtac agcttgaacg gatcttccag gtcatcgagg
     1441 cgcgcgccgg tgtcttcgtc tcggctgtcg atgatccagc gatacgcctg cagcaggacc
     1501 gcagggccga ggtagcgctc gccgttccac cagtagctcg ggcagctggt gctgcagcag
     1561 gcgcacagga tgcattcgta caggccatcg agcttggcgc ggtccgcctt cgactgcagg
     1621 cgctcgcggt ccggcggcgc cggggtctgg gtgcgcagcc acggcttgat cgaggcgtat
     1681 tgcgcgtaga aatgggtgag gtccgggacc aggtccttga ccaccggcat gtgcggcagc
     1741 gggtagatcg gcacctcggc attgcccttg ccgcaatccc cgatcgcctt ggtgcaggcc
     1801 agggtattgg tgccgtcgat gttcatcgcg cacgaaccgc agatgccctc gcggcaggag
     1861 cggcggaagg tcagggtcgg gtcgacctcg ttcttgatct tgatcagcgc gtccaggacc
     1921 atcggcccgc agctggcgag gtcgacgtcg taggtgtcca cccgcgggtt catgccgtcg
     1981 tcgggattcc agcggtagac cttgaaggtg cgcacctgct tcgcgccgga cgcggcggga
     2041 aagtgcttgc ccttcttgac ctgggagttc ttcgggaggg tgaattcggc catctcacga
     2101 ttcctgcttg tgtaggagcg ggccatgccc gcgatgcgct ttcagggtat tgcgatcgcg
     2161 cccatggggc gctcctacgg tcaatagacg cgcggcttcg gcgggaccac gtcgacgtcg
     2221 ttgctgagcg tgtacatgtg caccgggcgg tagtcgatgc gggtatcgcc ggcatcgtcg
     2281 acccagcaca cgctgtgctt gtgccagttg gcgtcgtcgc gctccgggaa gtcctcgcgc
     2341 gcatgcgcgc cgcgcgattc cttgcggttc tccgccgaga cgatggtggc cagcgcctgc
     2401 cccagcaggt tctgcagctc cagggtctcg accaggtccg agttccagac cagcgagcgg
     2461 tcggacacct tcacgtcggc gaaactggcg ttgatctcgc ggatcgccct gaccccgttc
     2521 gccagggttt cgccggtgcg gaacaccgcc gcgtcgttct gcatcgcccg ctgcatgttg
     2581 ttgcgaatgg ccgcggtgga ggtgccgcca ttggcattgc gcagcctgtc gaggttggcc
     2641 agcgccttgt cgcaggcgtc gcccgcgagt tgcttgtgcg gtgcgccggg ggtgatggtt
     2701 tcggcgcaac ggttcgccac cgcgcgcccg aacaccacca ggtccagcag cgagttggag
     2761 cccaggcggt tggcgccgtg caccgacacg caggcggcct cgccgatcgc gtacaggccg
     2821 ggcaccaccg cgtcggggtt gccgttgcag ggctgcacca cctcgccgtg gtagttggtc
     2881 gggatgccgc ccatgttgta gtgcacggtc gggatca
//
LOCUS       DAORUK010000207         2914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_48396_length_2914_cov_22.523260, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000207 DAORUK010000000
VERSION     DAORUK010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48396_length_2914_cov_22.523260"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PK818_10280"
     CDS             complement(<1..361)
                     /locus_tag="PK818_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQY83153.1"
                     /translation="MIAPTVAHLAFVRDDGQPLDYIPGQFIQVHFALADGALARRSYS
                     LATQHDHARAPGESVEIAVSYVPGGAATALFEGMPLGGSVDASGPFGRFCLLPNDANR
                     RYLLIGTGTGITPYRAML"
     gene            518..1477
                     /locus_tag="PK818_10285"
     CDS             518..1477
                     /locus_tag="PK818_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY83154.1"
                     /translation="MSLPSDGASPAPALRVSNLRKTYDNGVEALKGVSLDVAPGDFYA
                     LLGPNGAGKSTLIGIVSSLVNKSAGKVEVFGTDIDADRDGAMRLLGLVPQELNFNMFE
                     KPLDICVNYAGFYGIPRARALVRAEEELKRALLWDKADKMSRSLSGGMKRRLMIARAM
                     MTRPRLLILDEPTAGVDIEIRRGMWKTLKEINAAGTTIILTTHYLEEAENLCRNLAII
                     DHGRIVEEGPMRALLAKLDVEGFLFDIDGTLPAQLPRIEGTTLVASDDHTLDMDMPRA
                     MDLNRVFAAFEAAGIRVRSMRTKSNRLEELFVRLTGKQEGAQA"
     gene            1486..2268
                     /locus_tag="PK818_10290"
     CDS             1486..2268
                     /locus_tag="PK818_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY83155.1"
                     /translation="MTQTVARKNLVALGTIVRREIMRILRIWGQTLMPPAITMTLYFL
                     IFGGLIGSRVGQMDGIRYMDFIVPGLVMMSVIQNSYGNISSSFFGAKFGRHIEELLVS
                     PMPNWVILSGYVAGAVLRGVIVGAIVLCIAMLFTTVRIPHPLVMLGSVLLGATIFSLA
                     GFVNAVYAKKFDDIAIVPTFILTPLTYLGGVFYSVKLLPDWAEAATHANPVFYMVNAF
                     RYGLLGVSDVSLWVAFALMLGFVVVLGALGLWLLKRGTGMRS"
     gene            complement(2344..>2914)
                     /locus_tag="PK818_10295"
     CDS             complement(2344..>2914)
                     /locus_tag="PK818_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY83156.1"
                     /translation="EIFVELFYGFVLAMIAGILCIYMVLLLLFKHPMQPLTILTAIPL
                     CAGGAFGALLVTQNALSLPALIGLLMLIGIATKNSILLVDYAVMAEDLHGMSQHDALV
                     DACHKRARPVVMTTIAMGAGMLPLALGFSGDASFRAPMAWAVIGGLITSTALSLIVIP
                     AAYTVLHDIGDWVQRRFGKQDAPPASAQA"
BASE COUNT          484 a          974 c         1000 g          456 t
ORIGIN      
        1 gcagcatcgc ccggtacggg gtgatgccgg tgccggtgcc gatcagcagg tagcgccggt
       61 tggcgtcgtt cggcagcagg cagaagcgcc cgaacgggcc gctggcgtcg accgagccgc
      121 cgagcggcat gccctcgaac agggcggtgg cggcgccgcc gggaacgtag ctgaccgcga
      181 tctccacgct ttcgccgggc gcgcgggcgt ggtcgtgctg ggtcgccagc gagtagctgc
      241 ggcgcgccag tgccccatcg gccagtgcga aatggacctg gatgaactgg ccggggatgt
      301 agtccagcgg ctggccgtcg tcgcgcacga acgccaggtg cgccacggtg ggcgcgatca
      361 tgcgccggga gaccagtttc agcgggaagt ggacgatggc cacgcgatac tcgattcgat
      421 ggatgcaaca cagcgtggcg tcggtgcccg cgcgtggccg cctatactaa ccgcaacgtt
      481 ttcctgcctc gccgcgccgc gcggccgcgg agtcccgatg agcctgcctt ccgatggcgc
      541 gagccccgcg cccgcgctgc gcgtcagcaa cctgcgcaag acctacgaca acggcgtgga
      601 ggccctgaag ggggtcagcc tggacgtcgc ccccggggat ttctacgccc tgctggggcc
      661 gaacggcgcc ggcaagtcga ccctgatcgg gatcgtcagc tcgctggtca acaagtccgc
      721 gggcaaggtc gaggtgttcg gcaccgacat cgacgccgac cgcgacggcg cgatgcgcct
      781 gctcgggctg gtgccgcagg aactgaactt caacatgttc gagaagccgc tggacatctg
      841 cgtcaactac gcggggttct acggcatccc gcgggcacgg gcgctggtgc gcgcggagga
      901 ggagctcaag cgcgcgctgc tgtgggacaa ggccgacaag atgagccgct cgctgtccgg
      961 cggcatgaag cggcggctga tgatcgcccg cgcgatgatg acccgcccgc gcctgctgat
     1021 cctggacgag cccaccgccg gcgtggacat cgagatccgc cgcggcatgt ggaagacgct
     1081 gaaggagatc aacgccgccg gcaccaccat catcctgacc acgcactacc tggaggaagc
     1141 ggagaacctg tgccgcaacc tggcgatcat cgaccacggc cggatcgtcg aggagggccc
     1201 gatgcgcgcg ctgctggcca agctcgacgt cgagggcttc ctgttcgaca tcgacggcac
     1261 cctgccggcg caactgccgc ggatcgaggg caccaccctg gtcgccagcg acgaccacac
     1321 cctggacatg gacatgccgc gcgcgatgga cctcaaccgg gtgttcgccg cgttcgaggc
     1381 cgccggcatc cgcgtgcgct cgatgcgcac caagtcgaac cggctggagg agctgttcgt
     1441 gcgcctgacc ggcaagcagg aaggagcgca ggcatgagca ccgacatgac ccagaccgtc
     1501 gcccgcaaga acctggtcgc gctcggcacc atcgtccgcc gcgagatcat gcgcatcctg
     1561 cggatctggg gccagaccct gatgccgccg gcgatcacca tgaccctgta cttcctgatc
     1621 ttcggcggcc tgatcggatc gcgcgtgggg cagatggacg ggatccgcta catggacttc
     1681 atcgtccccg ggctggtgat gatgagcgtg atccagaaca gctacggcaa catcagcagt
     1741 tccttcttcg gcgccaagtt cggccgccac atcgaggaac tgctggtcag cccgatgccg
     1801 aactgggtga tcctctccgg ctacgtggcc ggggcggtgc tgcgcggggt gatcgtcggc
     1861 gcgatcgtgc tgtgcatcgc gatgctgttc accaccgtgc gcatcccgca cccgctggtg
     1921 atgctgggct cggtcctgct gggcgcgacg atcttctcgc tggccggttt cgtcaacgcg
     1981 gtgtacgcca agaagttcga cgacatcgcg atcgtcccca ccttcatcct gaccccgctg
     2041 acctacctcg gcggcgtgtt ctattcggtc aagctgctgc cggactgggc ggaggccgcg
     2101 acccacgcca acccggtgtt ctacatggtc aacgcgttcc gctacggcct gctgggcgtc
     2161 agcgacgtgt cgctgtgggt ggcgttcgcg ctgatgctgg gcttcgtggt ggtgctgggc
     2221 gcgctggggt tgtggctgct caagcgcggg accggcatgc gcagctgaga ccttgcatcg
     2281 acgcaacaag gaaacgcccg gcatcgccgg gcgctttttc catccgcgag ggtgacgcca
     2341 ccgctacgcc tgtgctgacg ctggcggcgc gtcctgcttg ccgaaccggc gctgcaccca
     2401 gtcgccgatg tcgtgcagca cggtgtaggc cgccgggatc acgatcaggc tgagcgcggt
     2461 ggaggtgatc aggccgccga tcaccgccca cgccatcggc gcgcggaagc tggcgtcgcc
     2521 cgagaacccg agcgcgagcg gcagcatgcc ggcgcccatc gcgatcgtgg tcatcaccac
     2581 cggccgcgcg cgcttgtggc aggcatcgac cagcgcatcg tgctggctca tgccgtgcag
     2641 gtcctcggcc atcaccgcgt agtcgaccag caggatcgag ttcttggtgg cgatgccgat
     2701 cagcatcagc aggccgatca gcgccggcag cgacaacgcg ttctgtgtca ccaacaacgc
     2761 accaaacgcg ccgccggcgc acagcgggat cgcggtgagg atggtcaacg gctgcatcgg
     2821 gtgcttgaac agcagcaaca gcaccatgta gatgcacagg atgccggcga tcatcgccag
     2881 cacgaagccg tagaacagtt cgacgaagat ctcg
//
LOCUS       DAORUK010000208         2892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_48964_length_2892_cov_18.878393, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000208 DAORUK010000000
VERSION     DAORUK010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48964_length_2892_cov_18.878393"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1609)
                     /gene="recJ"
                     /locus_tag="PK818_10300"
     CDS             complement(<1..1609)
                     /gene="recJ"
                     /locus_tag="PK818_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HQY83157.1"
                     /translation="MSAQRRIVRRTAVAPAGDWPAHWPPLLQRVHAARGSTDAQQALP
                     RLADLLPFQGMSGIDAAVDLLQDAIARDAHIVVVGDFDCDGATACAVGVRGLRMLGAT
                     RVSHAVPNRIVHGYGMTPGLVEELAGLQPDLLVTVDHGIACHAGIAAAKARGWQVLVT
                     DHHLPGSTLPDADAIVDPNQPGDTFASKAMAGVGVIFYLLLALRARRGVAADLASLLD
                     LVAVGTVADMVALDANNRALVGAGLRRLRAGQGCAGLRALIEVSGRDAASLGTTDIGY
                     AIGPRINAAGRLEDMGIGIECLLADDIGRARALAAVLHAINQERRGVQQQMTEEAETA
                     LQQAAGDPSPAVSLFDPDWHPGVVGLVASKLKERLHRPAIAFAPVEPGSAMLRGSARS
                     IPGFHVRDALALVDARHPGLIQRFGGHAMAAGLSLARADLPAFREAFVQVARETLTTD
                     LLTDTLHSDGALEAGDFGIETAIALRDGGHWGQGYPEPLFDGEFDVLGWRIVGERHLK
                     LELGLHGKRLNAIHFGGWDGDAPPSRVR"
     gene            complement(1614..2567)
                     /locus_tag="PK818_10305"
     CDS             complement(1614..2567)
                     /locus_tag="PK818_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HQY83158.1"
                     /translation="MPRLTLLLPATSRFAGAALPTALARALGRADRIHADAGEQAQLA
                     RQFRLLPDRWPHAALARAADAGVDDATPHAWLRADPAYIRPDINGARLLAIGHTLGID
                     QTDVDALLPALRPLFGDAGFTLDAAHPSRWYLRLPREARLPGFASPEAALGEDVFEHD
                     AFATAGNDADARRWRVLASEAQVLLHNHPHNAARAAAGRVPINALWFWGGGVLPDAIA
                     GAAPTLYSDDPLLHGIARVGRLQAMPLDDFANADGEALVDLRGARDPRALLERWLPPV
                     IDAVPKREVALDFADGAQFVLRASQRWRFWRSPLASLAPRG"
     gene            complement(2572..>2892)
                     /gene="greA"
                     /locus_tag="PK818_10310"
     CDS             complement(2572..>2892)
                     /gene="greA"
                     /locus_tag="PK818_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HQY83159.1"
                     /translation="QQGFIEGRIKHLEGELSHAHVIDIATLTAGDRVVFGATVQLADA
                     ETDEERTYQIVGDLEADIKLGLIAISSPVARALIGKHEGDVITIEAPAGTREYEIVSV
                     KYLD"
BASE COUNT          402 a         1040 c         1059 g          391 t
ORIGIN      
        1 tgcgcacgcg tgacggcggt gcgtcgccat cccagccgcc gaagtggatg gcgttcaggc
       61 gcttgccatg caggcccagt tccagcttga ggtggcgctc gccgacgatg cgccagccaa
      121 gcacgtcgaa ttcgccatcg aacagtggct cgggatagcc ctgtccccag tggccgccgt
      181 cgcgcagggc gatcgcggtt tcgatgccga aatcgcccgc ttccagcgcg ccgtcgctgt
      241 gcagggtgtc ggtcagcagg tcggtcgtca gcgtctcgcg ggcgacctgc acgaacgcct
      301 cgcgaaaggc cggcaggtcg gcgcgcgcca ggctcaggcc ggcagccatc gcatgcccgc
      361 cgaagcgctg gatcaggccg gggtggcggg cgtcgaccag ggccagcgca tcgcggacgt
      421 ggaagccggg aatcgagcgc gcggacccgc gcagcatggc gctgcccggc tccaccggcg
      481 cgaaggcgat ggccgggcgg tgcaggcgtt ccttcagctt cgacgcgacc agcccgacca
      541 cccccggatg ccagtccgga tcgaacaggc tgaccgccgg cgacggatcc cctgccgcct
      601 gctgcagcgc cgtctccgcc tcctcggtca tttgctgctg cacgccgcgg cgttcctggt
      661 tgatggcgtg caggaccgcc gccagcgcgc gcgcgcggcc gatgtcgtcg gcgagcaggc
      721 attcgatgcc gatgcccatg tcctccagcc ggccggcggc gttgatgcgc gggccgatcg
      781 catagccgat gtcggtggtg ccgaggctcg cggcatcgcg ccctgacacc tcgatcagtg
      841 cgcgcaagcc agcacagccc tgccccgcgc gcagccggcg caggccggcg cccaccagcg
      901 cgcggttgtt ggcgtccagc gccaccatgt ccgcgaccgt gccgaccgcc accaggtcca
      961 gcagcgaggc gaggtccgcg gccaccccgc ggcgggcacg caatgccagc agcaggtaga
     1021 agatcacccc gacgccggcc atcgccttgc tggcgaaggt gtcgccgggc tggttgggat
     1081 cgacgatggc atccgcgtcg ggcagcgtgc tgccgggcag gtggtggtcg gtcaccagca
     1141 cctgccagcc gcgtgccttc gccgccgcga tcccggcgtg gcaggcgatc ccgtggtcga
     1201 cggtgaccag caggtcgggc tgcaggcccg ccagttcctc gaccagtccc ggggtcatgc
     1261 cgtagccatg cacgatgcgg ttcggcaccg catgcgagac acgcgtcgcg ccgagcatgc
     1321 gcaggccgcg cacgccgacc gcgcaggcgg tggcgccgtc gcagtcgaag tcgccgacca
     1381 ccacgatgtg cgcgtcgcgg gcgatcgcgt cctgcagcag gtccaccgcg gcatcgatcc
     1441 cggacatgcc ctggaacggc agcaggtcgg ccagccgcgg cagcgcctgc tgcgcatcgg
     1501 tactgccgcg cgcggcgtgg acccgttgca gcagcggtgg ccagtgcgcg ggccagtcgc
     1561 ctgccggcgc caccgccgtg cggcgaacga tgcggcgttg ggcgctcatg cggtcatccc
     1621 cgcggggcca gcgatgcgag cgggctgcgc cagaaccgcc agcgctggct ggcgcgcagc
     1681 acgaactgcg cgccgtcggc gaaatcgagc gcgacttcgc gcttcggcac cgcgtcgatg
     1741 accggcggca gccagcgttc gagcagtgca cgtggatccc gtgcgccgcg caggtcgacc
     1801 agtgcctcgc catcggcatt cgcgaaatcg tccagcggca tggcctgcag cctgccgacg
     1861 cgtgcgatgc cgtgcagtag cggatcgtcg gaatacagcg tcggcgcagc gcccgcgatg
     1921 gcgtcgggca agacgccgcc accccagaac cacagggcat tgatcggcac cctgccggcg
     1981 gctgcgcgcg ctgcgttgtg cgggtggttg tgcaggagca cctgcgcctc gctggccagc
     2041 acgcgccagc gacgggcatc ggcgtcgttg ccggcggtgg cgaaggcgtc atgctcgaac
     2101 acgtcctcgc ccagcgcggc ctcgggtgac gcgaacccgg gcaagcgggc ctcgcgcggg
     2161 aggcgcaggt accagcgcga cgggtgcgcg gcgtccaggg tgaaaccggc atcgccgaac
     2221 agcggacgca gcgccggcaa caaggcatcg acgtcggtct ggtcgatgcc gagcgtgtgg
     2281 ccgatcgcca gcaggcgcgc gccgttgatg tcgggacgga tgtacgccgg gtcggcgcgc
     2341 agccacgcat gcggcgtcgc gtcgtcgacc ccggcatcgg cggcacgggc cagcgccgcg
     2401 tgcggccagc gatccggcag cagccggaac tgccgggcca gctgcgcctg ctcgccggcg
     2461 tccgcgtgga tgcggtccgc gcggcccagc gcgcgcgcca gcgccgtggg caacgctgca
     2521 cccgcgaagc ggctcgtcgc cggcaacagc agcgtcagcc gcggcaccgg ctcagtccag
     2581 gtacttgacc gagacgatct cgtattcgcg ggtgccggcc ggtgcctcga tggtgatcac
     2641 gtcgccctcg tgcttgccga tcagcgcgcg ggccaccggc gaggagatcg cgatcaggcc
     2701 cagcttgatg tcggcctcga ggtcgccgac gatctggtag gtgcgctcct cgtcggtctc
     2761 ggcgtcggcc agttgcacgg tggcgccgaa taccacccgg tcgccggcgg tgagggtggc
     2821 gatgtcgatc acgtgcgcat gcgacagctc gccttccagg tgcttgatgc ggccctcgat
     2881 gaagccctgc tg
//
LOCUS       DAORUK010000209         2869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_49610_length_2869_cov_18.431770, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000209 DAORUK010000000
VERSION     DAORUK010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49610_length_2869_cov_18.431770"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PK818_10315"
     CDS             complement(<1..259)
                     /locus_tag="PK818_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY83160.1"
                     /translation="MTHTPSPHARTDLDIASNSTAPVQAADASTPARGSLRTRAVGKT
                     VALPDSQLTILDGISLDIAAGERVAIVGESGSGKTTLLSLLA"
     gene            258..836
                     /locus_tag="PK818_10320"
     CDS             258..836
                     /locus_tag="PK818_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HQY83161.1"
                     /translation="MLLILAAPGALARELKVLVMGDSLSAAYGLRAEQGWVALADAKS
                     ARYAFHNASISGETSAGGRSRIGAELERVRPDIVIIELGANDGLRGLPLQQLGANLGW
                     MIGASKAAGARVLLVGMQLPPNFGRYASDFERSYLLISQRLQTGLLPRFLAPLGTERK
                     WFQADNLHPVAEAQPLLAAQVLQALDGLPPPR"
     gene            complement(840..2078)
                     /locus_tag="PK818_10325"
     CDS             complement(840..2078)
                     /locus_tag="PK818_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HQY83162.1"
                     /translation="MDWDVSIASLSTALGIGLLVGTVRERHHAPDRIGKAGLRTHALL
                     ALTGALAAGFGTGALLVALAAVAALAVAGYRAGGATHDPGLTGEVAMLVSVLLGAVAQ
                     QSPALAAGLGVLVAALLWAKAPLRRLSQELISEREVQDGLLLAASALVILPLLPDRAV
                     DPWGVVQLSMLWKIVVLIMATGMLGHVARRAVGARRGLPVAGFFSGFASSTAAVVSFG
                     HLSRAQPALRAASASAALLANLASVLLLVAVVAAVSPTLAQAIAWPMASACAGLLLVA
                     AHGLLRGNADGLPAGPEPRAFRISHALLIALVIAVVMLLSEALHRAFGDAGAMTGAIV
                     AALAELQAAAASVARMASTGSLSAAQAAWGMVGLLAAMSIAKSVIAFVSGGGAYGRQV
                     TAGLVLMTAAGAAGTWLTTR"
     gene            complement(2109..>2869)
                     /gene="purD"
                     /locus_tag="PK818_10330"
     CDS             complement(2109..>2869)
                     /gene="purD"
                     /locus_tag="PK818_10330"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HQY83163.1"
                     /translation="FGDAGARVVIEEFLDGEEASFISMVDGHTALPMATSQDHKRVGD
                     GDTGPNTGGMGAYSPAPVVTPEVHARVMREVVNPTVQGMIKDGIPFTGFLYAGLMIDA
                     DGAPKVIEFNVRFGDPETQPVMLRLQSDLVGLLEAAIDGTLDGVEAQWDPRPSLGVVM
                     AASPYPETPITGDVISGLDAVPAHAKVFHAGTALDAAGNVIATGGRVLCVCALGDSVS
                     AAQRAAYAGVEAISWAHAFHRHDIGWRAIARERG"
BASE COUNT          425 a         1048 c         1019 g          377 t
ORIGIN      
        1 cggccagcag cgagagcagg gtggtcttgc cggaaccgga ctcgccgacg atcgcgaccc
       61 gttcgccggc ggcgatgtcc aggctgatgc cgtcgaggat ggtcagctga ctgtccggta
      121 acgccacggt cttgccgacc gcgcgcgtgc gcaggctgcc gcgcgcaggc gtgctagcgt
      181 ctgcggcctg tacgggagcc gtggagttgc tggcgatgtc gaggtcggtg cgcgcgtggg
      241 ggctgggtgt gtgcgtcatg ctgctgatcc tagccgcgcc gggcgcgctt gcgcgtgagc
      301 tgaaggtact ggtcatgggc gattcgctgt ccgcggccta tggcctgcgc gcggagcagg
      361 gctgggtggc gctggccgat gcgaagtcgg cgcgctacgc cttccacaac gccagcatct
      421 ccggcgagac cagcgccggc gggcgcagcc ggatcggtgc cgagctcgaa cgggtgcggc
      481 cggacatcgt gatcatcgaa ctgggcgcca acgacggcct gcgcggcctg ccgctgcagc
      541 agctgggcgc caacctgggc tggatgatcg gggcctcgaa ggccgccggt gcgcgggtgc
      601 tgctggtggg catgcagctg ccgccgaatt tcggccgcta tgccagcgac ttcgagcgca
      661 gttacctgct gatcagccag cgcctgcaga ccggcctgct gccgcggttc ctggccccgc
      721 tgggcaccga gcgcaagtgg ttccaggccg acaacctgca cccggtggcg gaagcgcagc
      781 cgttgctggc cgcgcaggtg ctgcaggcgc tggacgggtt gccgccgccg cgctgagcgc
      841 tagcgggtgg tgagccaggt gcccgccgcg ccggccgcgg tcatcaacac cagtcccgcg
      901 gtgacctggc gtccgtaggc gccgccgccg ctcacgaagg cgatcacgct cttggcgatg
      961 gacatcgccg caagcaggcc caccatcccc catgccgcct gcgcggcgga caggctgccg
     1021 gtcgaagcca tccgcgccac gctggcagct gccgcctgca actccgccag cgccgccacg
     1081 atggcgccgg tcatcgcccc ggcatcgccg aacgcgcggt gcagcgcttc ggacagcagc
     1141 atcaccacgg cgatcaccag cgcgatcagc agtgcgtggc tgatgcggaa cgcgcgcggt
     1201 tccggtcccg ccggcagtcc gtccgcgttc ccgcgcagca ggccgtgcgc cgcgaccagc
     1261 agcaggccgg cgcaggcgct ggccatcggc catgcgatcg cctgcgccag cgtcggcgag
     1321 accgccgcca ccaccgccac cagcagcagc accgaggcga ggttggccag cagcgcggcg
     1381 gatgccgatg cggcgcgcag cgcgggctgc gcacgcgaca gatggccgaa gctgaccacc
     1441 gccgcagtcg atgaagcgaa gccggaaaag aagccggcca ccggcaggcc ccggcgtgca
     1501 ccgaccgcac gccgcgcgac gtgcccaagc atcccggtgg ccatgatcag caccacgatc
     1561 ttccacaaca tggacagctg caccacgccc cacggatcca ccgcccggtc cggcagcagc
     1621 ggcaggatga ccagcgcgga cgccgccagc agcaggccgt cctggacctc gcgctcgctg
     1681 atcagttcct ggctcagccg gcgcagcggc gccttcgccc acagcagggc cgcgaccagc
     1741 acccccagac ccgccgccag cgccggcgac tgctgcgcca ccgcgcccag cagcacggaa
     1801 accagcatcg ccacctcgcc ggtgaggccg gggtcatgcg tggcgccgcc ggcccgatac
     1861 cccgcgaccg cgagcgccgc gacggcggcc agtgcgacca gcagtgcacc ggtgccgaag
     1921 ccggcggcca gtgcgccggt cagggcgagc agggcatgcg tgcgcaggcc ggccttgccg
     1981 atccggtccg gggcgtggtg acgctcgcgc acggtgccga ccagcagccc gatcccgagc
     2041 gcggtgctga gtgaagcgat gctgacatcc cagtccatgc ccggatgatg cctgttttca
     2101 ggcgcgtgtc agccccgttc gcgcgcgatc gcacgccagc cgatgtcgtg gcggtggaac
     2161 gcgtgcgccc acgaaatcgc ctccacgccg gcataggccg cgcgctgcgc ggccgacacc
     2221 gaatcgccca gcgcgcacac gcacagcacg cggccgccgg tggcgatgac gttgcccgca
     2281 gcgtccagcg cggtgccggc atggaacacc ttggcatgcg cgggcacggc gtcgaggccg
     2341 gagatcacgt cgccggtgat cggcgtttcc gggtacgggc tggcggccat caccacgccc
     2401 agcgacgggc gcgggtccca ttgcgcttcg acgccatcga gggtgccgtc gatggcggcc
     2461 tcgagcaggc ccaccaggtc cgattgcagg cgcagcatga ccggctgggt ttccggatcg
     2521 ccgaaacgga cgttgaactc gatcaccttg ggcgcgccgt cggcgtcgat catcagcccg
     2581 gcgtagagga agccggtgaa cggaatgccg tccttgatca tcccctgcac ggtgggattg
     2641 accacctcgc gcatcacccg cgcatgcacc tccggcgtga ccaccggtgc cggcgagtag
     2701 gcgcccatgc cgccggtgtt cgggccggtg tcgccgtcgc ccacccgctt gtggtcttgc
     2761 gaggtcgcca tcggcagtgc cgtgtggccg tccaccatcg agatgaagct ggcctcctcg
     2821 ccatcgagga attcctcgat gaccacgcgc gcgccggcat cgccgaagg
//
LOCUS       DAORUK010000210         2842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_50392_length_2842_cov_18.945820, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000210 DAORUK010000000
VERSION     DAORUK010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50392_length_2842_cov_18.945820"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1687)
                     /locus_tag="PK818_10335"
     CDS             complement(<1..1687)
                     /locus_tag="PK818_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQY83164.1"
                     /translation="MSRFDTNARTVLFATLLAALPALAQDPQVKPAEVRREVVGNRTS
                     ENIPQIPAALVEQLNRYQNTRGAGLAGWTRDGCLLISTRFAETAQAHRVCQPQGMREQ
                     LTFYPEPVGGITPAPAKAWRDGFVFAKDKGGDEFSQLYWFDAATRATTLLTDGKRSQN
                     GGTTLSRDGGLMAYASTARNGTDRDIWVRDTRTGASRALVTAGGSWGAMDFSPDGREL
                     LVMKYVSAAESYPGVVDVASGKLTMFPVDGGKASFGGFAFAPDGKAVYFVSDEPLQGR
                     PQEFKTLRYHDPASGRLEVLSGTIPWDVGGFTIADDGKHLAFASNEDGISKLRVLSLP
                     GHEEVRLPALPIGVIGGLSFSPDGKRLALTLNSATSPSDVHVIDLAGATLTRWTQSEV
                     GGLDAANFVAPTLVRYPTFDTVDGRQRTIPAFYYKPAKPSRTGKYPVVINIHGGPEGQ
                     SLPTFSSTAQYMANELGVAMLVPNVRGSSGYGKTYLSLDNAAKREDSVKDIGALLDWI
                     ATQPELDASRVGVIGGSYGGYMVLASLTHYSDRIRAGVDIVGISHFGTFLKNTE"
     gene            complement(1763..>2842)
                     /locus_tag="PK818_10340"
     CDS             complement(1763..>2842)
                     /locus_tag="PK818_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HQY83165.1"
                     /translation="EDSPKYLNSPETALFHKGRELYGLWQAKQANARLDRLIVVEGYM
                     DVVALAQYGVSQAVATLGTATTPDHAELLFRSAPDVFFCFDGDRAGRSAAWKALESVL
                     PRMKDGRQAMFLFLPDGEDPDSIVRQEGVDGFEARLRAATPLSEYFYASLSSDVNLSS
                     LDGKARLAERCKPLLAQIPDGAFGDLMQQRLTELTGVGARASAPQTHLPAQRLNRAPP
                     AGAQKRSLVRSAIESLLHRPALALELQPPYRFAALRQPGVELLSELVLLVRERPEIST
                     GALLEHFAGREEAGALQKLATHTLPGEEDSQRDEFLGAIAQLGKQVEQQRIDELNARI
                     RNQGLSSLSAEEKAELRELQSPRRA"
BASE COUNT          406 a          958 c         1008 g          470 t
ORIGIN      
        1 tctcggtgtt cttcaggaag gtgccgaagt gcgagatgcc gacgatgtcc acgccggcgc
       61 ggatgcggtc gctgtaatgc gtcagcgacg ccagcaccat gtagccgccg tagctgccgc
      121 cgatcacgcc cacgcggctg gcatcgagtt cgggctgcgt ggcgatccag tcgagcaggg
      181 cgccgatgtc cttgaccgaa tcctcgcgct tcgccgcgtt gtccagcgac agataggtct
      241 tgccgtagcc gctggagccg cgcacgttcg gcaccagcat cgccacgccc agctcgttgg
      301 ccatgtattg cgcagtggaa ctgaaggtgg gcagcgactg cccctccggg ccgccgtgga
      361 tgttgatcac caccgggtac ttgccggtcc tggagggctt ggccggcttg taatagaacg
      421 ccgggatggt gcgttgcctg ccatcgaccg tgtcgaaggt cgggtagcgc accagcgtcg
      481 gggcgacgaa gttcgccgcg tccagcccgc cgacttcact ctgcgtccag cgcgtcaaag
      541 tggccccggc aaggtcgatc acgtgcacgt cgctgggtga ggtcgcgctg ttcagggtca
      601 gcgccaggcg cttgccgtcc ggcgagaacg acagcccgcc gatcacgccg atcggcagcg
      661 cgggcaggcg gacttcctcg tggccgggca gcgacagcac gcgcagcttg ctgatgccgt
      721 cctcgttgct ggcgaacgcg aggtgcttgc cgtcgtcggc gatggtgaag ccgcccacgt
      781 cccacgggat ggtgccggag agcacttcga gcctgccgga cgcggggtcg tggtaacgca
      841 gggtcttgaa ctcctgcggc ctgccctgca gcggttcgtc ggagacgaaa tacacggcct
      901 tgccatcggg ggcgaaggcg aagccgccga agctggcctt gccgccgtcg acagggaaca
      961 tcgtgagctt gccgctggcc acgtcgacca cgcccgggta cgactccgcg gcagacacgt
     1021 acttcatcac caacagctcg cggccgtccg gcgagaaatc catcgcgccc cagctgccgc
     1081 cggcggtgac cagcgcgcgg ctggcgccgg tgcgggtgtc gcggacccag atgtcgcggt
     1141 cggtgccgtt gcgcgcggtg ctggcgtagg ccatcaggcc gccgtcgcgg ctcagggtgg
     1201 tgccgccgtt ctggctgcgc ttgccgtcgg tgagcagggt ggtggcgcgg gtggcggcgt
     1261 cgaaccagta cagctggctg aattcgtcac cgcccttgtc cttggcgaac acgaagccgt
     1321 cgcgccaggc cttggccggc gcgggggtga tgccgccgac cggctccgga tagaacgtca
     1381 gctgttcgcg catgccctgc ggctggcaga cccggtgcgc ctgcgcggtc tcggcgaagc
     1441 gggtgctgat cagcaggcag ccatcgcggg tccagccggc caggcccgcg ccgcgggtgt
     1501 tctggtagcg gttgagctgt tcgaccagtg ccgccgggat ctgcgggatg ttctcgctgg
     1561 tgcggttgcc gacgacttcg cgcctgacct cggccggctt cacctgcggg tcctgggcaa
     1621 gggcgggcag tgccgcgagc agggtggcga acaggacggt tcgggcattc gtgtcgaaac
     1681 gggacatggc ggcttcccag gccgggtgga accgcgagct tagcgcgatg acgcgccgcg
     1741 gcgccccccg aaggtcatgc gctcaggccc ggcgcgggga ctgcagttcg cgcagctcgg
     1801 ctttctcttc ggccgacagc gacgacaggc cctggttgcg gatgcgcgcg ttgagctcgt
     1861 cgatgcgctg ttgctcgacc tgcttcccga gttgcgcaat cgcgcccagg aactcgtcgc
     1921 gctggctgtc ttcctcgccg ggcagcgtgt gcgtggccag cttctgcagg gcgcctgctt
     1981 cctcgcgccc ggcgaaatgc tcgagcagcg cgccggtgct gatctccggg cgctcgcgca
     2041 ccagcagcac cagttccgaa agcagctcca cgccgggctg gcgcagcgcc gcgaagcgat
     2101 agggcggttg cagttccagc gccagcgccg ggcgatgcag cagggattcg atcgcgctgc
     2161 gcaccaggct gcgtttctgc gccccggccg gtggcgcgcg attcaaccgc tgcgcaggca
     2221 ggtgggtttg cggcgcgctg gcgcgcgcgc cgacgccggt gagttcggtc aggcgttgct
     2281 gcatcaggtc gccgaacgcg ccatcgggaa tctgcgcaag cagcggcttg cagcgttcgg
     2341 ccagccgcgc cttgccgtcc aggctggaca ggttcacgtc actggacaac gacgcgtaga
     2401 agtactccga cagtggggtg gcggcgcgca ggcgtgcctc gaaaccgtcc acgccttcct
     2461 gccgcacgat cgaatccgga tcctcgccat ccggcaggaa caggaacatc gcctggcggc
     2521 catccttcat ccgcggcagc acggattcca gcgccttcca tgccgcgctg cggccggcac
     2581 ggtcgccatc gaagcagaag aacacgtcgg gtgcgctgcg gaacagcagc tcggcatggt
     2641 ccggggtggt cgcggtgccc agcgtggcca ccgcctgcga caccccgtac tgggccagcg
     2701 ccaccacgtc catgtagccc tcgaccacga tcagccggtc gagcctggcg ttcgcctgtt
     2761 tcgcctgcca caggccatac aactcgcggc ccttgtggaa cagcgcggtc tccggcgagt
     2821 tgaggtactt cggcgaatcc tc
//
LOCUS       DAORUK010000211         2758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_52892_length_2758_cov_20.706622, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000211 DAORUK010000000
VERSION     DAORUK010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52892_length_2758_cov_20.706622"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PK818_10345"
     CDS             <1..668
                     /locus_tag="PK818_10345"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892689.1"
                     /note="catalyzes the formation of agmatine from arginine
                     in putrescine and spermidine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HQY83166.1"
                     /translation="IDLVQRARIDDLFYAIAHAVKARLTYDEKSHRDLLDELNERLVD
                     KYFVNFSVFESMPDVWAIDQVFPIVPIERLDERPTRRGIIADLTCDSDGKIDTYVENE
                     DLDSSLPLHELRPGERYRIGFFLIGAYQEVLGDIHNLFGDTDAIEVKLNGDGYAITQQ
                     RRGDTTDVMLDYVGYKLDDLRRRYRALVGAAELGRGEAERLNEALERGLTAYTYLSDE
                     PLE"
     gene            complement(672..1418)
                     /locus_tag="PK818_10350"
     CDS             complement(672..1418)
                     /locus_tag="PK818_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQY83167.1"
                     /translation="MTRTAFITGATSGFGRAAAKRFIEAGWQVVACGRRADRLQALRD
                     ELGDALHPAAFDIRDEAAMRAALDALPERFRGIDLLVNNAGLAQGTRPAQDALLSDWK
                     TMIDTNVTALVTLTHALLPVLIARRGAIINVSSTAATYPYPGGNAYGGSKAFVSQFSL
                     GLRADLHGTGVRVTTLEPGMAETEFTLVRTHGDQAASDALYRDAEPMTADDIAGTIFW
                     IATLPPHLNVNRLELMPTRQSFAGFQVHRG"
     gene            complement(1461..2222)
                     /locus_tag="PK818_10355"
     CDS             complement(1461..2222)
                     /locus_tag="PK818_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014091287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQY83168.1"
                     /translation="MAARRGKSQARRNGSNGLPGWAWLVIGVLVTLLVVLAAPRLLKG
                     GDGDGFLRIGPKPNPDALPAAVDENGDAIAPDVATEAPAAADGAKPKPTQYDFYTLLP
                     GEGVQMTEAELAASAQAEADAQARAMQTATQAPGAADTATLPQPIAETPAAGATDAGT
                     AATPAEASGAAAAPASDARYILQAGAFGASGDAETVKAKIAMLGLNARVESAQIGGKT
                     VYRVRMGPYGSASELAEAKAKLAGGGLPAMAVKTN"
     gene            complement(2223..>2758)
                     /locus_tag="PK818_10360"
     CDS             complement(2223..>2758)
                     /locus_tag="PK818_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HQY83169.1"
                     /translation="KLSKRAGSYLTLRDLIEETSADAVRWFLVARKPDSQLTFDIDLA
                     RSQSNDNPVYYVQYAHARICSLLRQAGEKGYAHDAAHGEASLHLLDDAPAQELMRELS
                     RFAEVVEAAGATLEPHLLAQYLRELANAFHGWYHASPVLVEDGPLRDARLALALATRQ
                     VLANGLDLLGVSAPEKM"
BASE COUNT          401 a          942 c          981 g          434 t
ORIGIN      
        1 agatcgacct ggtccagcgc gcgcgcatcg acgacctgtt ctacgccatc gcccacgcgg
       61 tgaaggcgcg cctgacctac gacgagaaga gccaccgcga cctgcttgac gaactcaacg
      121 agcggctggt cgacaagtac ttcgtcaact tcagcgtgtt cgagtcgatg cccgacgtct
      181 gggcgatcga ccaggtgttc ccgatcgtgc cgatcgagcg gctcgacgaa cggccgaccc
      241 ggcgcggcat catcgccgac ctgacctgcg actccgacgg caagatcgac acctacgtcg
      301 agaacgaaga cctcgacagc tcgctgccgc tgcacgagct gcgcccgggc gagcgctacc
      361 ggatcggctt cttcctgatc ggcgcctacc aggaagtgct cggcgacatc cacaacctgt
      421 tcggcgacac cgatgcgatc gaggtgaagc tcaatggcga cggctatgcg atcacccagc
      481 agcgccgcgg cgataccacc gacgtgatgc tggactacgt cggctacaag ctggacgacc
      541 tgcggcgccg ctaccgggcg ctggtcggcg ccgccgagct cggcaggggc gaggcggagc
      601 ggctcaacga agcgctggaa cgcggcctga ccgcgtacac ctacctgagc gacgaaccgc
      661 tggagtgacg cctagccgcg atggacctgg aagccggcga aggattgccg ggtcggcatc
      721 agttccagcc ggttgacgtt gaggtgcggt ggcagcgtcg caatccagaa aatggtgccg
      781 gcgatgtcgt ccgcggtcat cggctcggca tcccggtaca gcgcatccga tgccgcctgg
      841 tcgccgtggg tgcggaccag ggtgaactcg gtttcggcca tgcccggttc cagcgtggtc
      901 acgcgcacgc cggtgccgtg caggtcggcg cgcaggccca gcgagaactg cgacacgaag
      961 gccttgctgc cgccgtaggc attgccaccg gggtagggat aggtggcggc ggtggagctg
     1021 acgttgatga tcgcgcccct gcgtgcgatg agcaccggca acagcgcgtg ggtcagcgtc
     1081 accagcgcgg tgacgttggt gtcgatcatg gtcttccagt cggacagcag cgcgtcctgc
     1141 gcgggccggg tgccctgcgc caggccggcg ttgttgacca gcaggtcgat gccgcggaag
     1201 cgttccggca gcgcgtcgag cgcggcgcgc atcgcggctt cgtcgcggat gtcgaaggcg
     1261 gccgggtgca gggcgtcgcc cagctcgtcg cgcagcgcct gcaggcggtc ggcgcggcgg
     1321 ccgcaggcga cgacctgcca gccggcttcg atgaagcgtt tggcggcggc gcggccgaac
     1381 ccggaggtgg cgccggtgat gaatgcggtc ctggtcatgc cggtgattct agatggcacg
     1441 cggcagccgc ggcggtgcca tcagttggtc ttcaccgcca tcgccggcag gccgccgccg
     1501 gcgagcttcg ccttcgcctc ggccagttcc gatgcgctgc catacggccc catgcgcacg
     1561 cgatagacgg tcttgccgcc gatctgcgcg gactccacgc gggcgttcag cccgagcatc
     1621 gcgatcttgg ccttgaccgt ctccgcatcg ccggatgcgc cgaacgcgcc ggcctgcagg
     1681 atgtagcgcg catcactcgc cggggccgcg gcggcgccgc tggcctcggc cggcgtggcg
     1741 gcggtgcctg catcggttgc gccagccgcc ggggtttccg cgatgggctg cggcagggtt
     1801 gcggtatcgg ccgcgcccgg tgcctgcgtg gccgtttgca tggcgcgtgc ctgcgcatcc
     1861 gcctcggcct gcgcactggc ggccagttcg gcctcggtca tctgcacgcc ctcgcccggc
     1921 agcagggtgt agaagtcgta ctgggtgggc ttcggcttgg cgccgtcggc cgcggctggc
     1981 gcctcggtgg cgacgtcggg cgcgatcgcg tcgccgttct cgtcgaccgc cgccggcaat
     2041 gcatccgggt tcggcttcgg gccgatgcgc aggaagccgt cgccatcgcc gcccttgagc
     2101 aggcgcgggg cggccagcac caccagcagg gtgaccagga cgccgatcac cagccaggcc
     2161 cagcccggca ggccgttgga accattgcgt cgtgcctgcg acttgccgcg tcttgctgcc
     2221 atttacatct tctccggtgc gctgacgccg agcaggtcca gcccattggc caggacctgc
     2281 cgcgtcgcca aggccagggc aagccgcgcg tcgcgcagcg gaccgtcctc caccagcacc
     2341 ggcgaggcgt ggtaccagcc gtggaacgca ttggccagtt cgcgcaggta ctgcgccagc
     2401 agatgcggtt ccagcgtcgc gccggcggct tcgaccacct ctgcaaagcg cgacagctcg
     2461 cgcatcagct cttgcgccgg ggcgtcgtcc agcaggtgca gcgaggcctc gccgtgcgcg
     2521 gcgtcatggg cgtagccctt ctcgccggcc tggcgcagca ggctgcagat gcgcgcgtgc
     2581 gcgtactgca cgtagtagac cgggttgtcg ttcgactgcg agcgggccag gtcgatgtcg
     2641 aaggtcagct gggaatccgg cttgcgcgca accaggaacc agcgcaccgc atcggcactt
     2701 gtctcctcga tcaggtcgcg cagggtcagg tagctgccgg cgcgcttgga cagcttca
//
LOCUS       DAORUK010000212         2604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_58222_length_2604_cov_23.403688, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000212 DAORUK010000000
VERSION     DAORUK010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58222_length_2604_cov_23.403688"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PK818_10365"
     CDS             complement(<1..305)
                     /locus_tag="PK818_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HQY83170.1"
                     /translation="MAAKDIRFGEDARAKMVRGVNVLANAVKATLGPKGRNVVLQKSY
                     GAPTITKDGVSVAKEIELADAFENMGAQMVKEVASKTSDNAGDGTTTATVLAQAFIR"
     gene            complement(408..695)
                     /gene="groES"
                     /locus_tag="PK818_10370"
     CDS             complement(408..695)
                     /gene="groES"
                     /locus_tag="PK818_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HQY83171.1"
                     /translation="MSNIKPLYDRVVIKRMEEEKLSAGGIVIPDSATEKPIKGEVIAV
                     GAGKVLDNGQLRAPQVKVGDTVLFGKYSGTEVKLDGQELLVVKEDDIFAIL"
     gene            899..1228
                     /gene="cutA"
                     /locus_tag="PK818_10375"
     CDS             899..1228
                     /gene="cutA"
                     /locus_tag="PK818_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HQY83172.1"
                     /translation="MRMPVLICYCTCPDAATAGRIATALVDERLAACVNMLPGVRSVY
                     RWQGQVEAADEVLLLVKTTAEAYPALQDRVRALHPHELPELLAVEPAFGLPGYLSWVT
                     DETRRKD"
     gene            1233..>2604
                     /locus_tag="PK818_10380"
     CDS             1233..>2604
                     /locus_tag="PK818_10380"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HQY83173.1"
                     /translation="MPLSTFRRRLAGFLLVLALPASAVAQQVDLPPVDEVFVLSAQAT
                     APDRIEVRWRIAEGYYLYRHRTSVKADAAFAGASLSLPKGKAYRDEFFGDVETYRQQL
                     VGTLDGTPAGASATLTVKYQGCADAGICYPPQTRALKVALPVAADAQDAAAGAGFGTR
                     KGGLFAVGNADAGATDAAPLPVAQAFTTEVIADGGNTLLLRLTPARGYYIYRDKLSVA
                     LDAGTGLSAGLPPVSRLPKATPYRDEHFGNVAVYFEQVEIAVPVTRTVATAAKGTLLL
                     GLQGCQDGGICYPPMIRRLAVSLPAGTVTPVAPDAPALGADTAPVASAAAAATGTDAT
                     ASPAATGTFAATAGPDPVRTPPPQRQAHGLLASLLLALFGGLILNLMPCVLPVLSLKA
                     LSLAQSGESRERARRHALAYTAGVLASFAALGALALALRKAGLVLGWGFQLQQPLVVA
                     LLALVVF"
BASE COUNT          355 a          927 c          889 g          433 t
ORIGIN      
        1 tcgcggatga acgcctgcgc cagcacggtg gcggtggtgg tgccgtcacc ggcgttgtcg
       61 gaggtcttgg aggcgacttc cttcaccatc tgcgcgccca tgttctcgaa cgcgtcggcc
      121 agctcgattt ccttggcgac ggacacgccg tccttggtga tcgtcggcgc gccgtagctc
      181 ttctgcagca cgacgttgcg gcccttcggg cccagcgtgg ccttgacggc attggcgagc
      241 acgttgacgc cgcgcaccat cttggcgcgt gcgtcttcgc cgaaacgaat gtcttttgca
      301 gccattggaa atcacctcga atgtggggcg ggctcaaccc gccgctctgg aatggggatg
      361 tctcggtggc gggccgtggc ccgccgtgca acggaagcgc cgcgcgatca gaggatcgcg
      421 aagatgtcgt cttccttgac caccagcagt tcctggccgt ccagcttgac ctcggtgccg
      481 ctgtacttgc cgaacaggac cgtgtcgccg accttgacct gcggcgcgcg cagctggccg
      541 ttgtccagca ccttgccggc gccaacggcg atgacttcgc ccttgatcgg cttctcggtg
      601 gccgaatccg ggatcacgat cccgccggcg gacaacttct cttcttccat gcgcttgatg
      661 accacgcggt cgtacagcgg cttgatattg gacatggcaa cctcggtaaa tggttgattg
      721 aagtggaaaa acccggcgat tttagcagtc gggctggccg agtgccaaat cgcggacgaa
      781 gaaaccgggc gaacccggat gccagcaaga tggggcgccg ccgatgcctt tcaagggccg
      841 ccgcccacaa acgttgcccc ggccggcagg ttcgcccggg cgggcctgcc gctaccctat
      901 gcggatgcct gtcctgatct gctactgcac ctgccccgac gccgccaccg ccgggcgcat
      961 cgcgaccgcg ctggtggacg aacgcctggc cgcctgcgtg aacatgctgc cgggcgtgcg
     1021 ctcggtctac cgctggcagg gccaggtgga ggcggcggac gaggtgctgc tgttggtcaa
     1081 gaccaccgcc gaggcctacc ctgcgctgca ggatcgcgtg cgtgcattgc acccgcatga
     1141 actgccggag ctgctggcgg tcgaacccgc cttcgggctg cccgggtacc tgtcctgggt
     1201 caccgacgaa acccgccgca aggattgagc gcatgccgtt gtccaccttc cgccgccggc
     1261 tggccggctt cctgctggtg ctggcgttgc ccgccagcgc agtcgcccag caggtcgacc
     1321 tgccgccggt cgacgaggta ttcgtgctgt ccgcgcaggc caccgcgccg gaccgcatcg
     1381 aggtccgctg gcggatcgcc gaaggctact acctgtaccg gcaccgcacc tcggtcaagg
     1441 ccgacgccgc cttcgccggc gccagcctgt ccctgccaaa gggcaaggcc taccgcgacg
     1501 agttcttcgg cgatgtcgaa acctaccggc agcagctggt cggcaccctc gacggcaccc
     1561 cggcgggcgc ctccgccacc ctgaccgtca agtaccaggg ctgcgcggat gccggcatct
     1621 gctatccacc gcagacccgc gcgctcaagg tcgcgctgcc ggtcgcggcg gatgcccagg
     1681 acgcggccgc cggcgccgga ttcggcaccc gcaagggcgg cctgttcgct gtcggcaatg
     1741 ccgatgccgg cgccaccgac gccgcgccgc tgccggtggc gcaggccttc accaccgagg
     1801 tgattgccga tggcggcaac accctgctgc tgcggctgac cccggcgcgc ggctactaca
     1861 tctaccgcga caagttgtcg gtggcgctgg acgcaggcac cggcctgtcg gcgggcttgc
     1921 cgccggtctc gcgattgccg aaggcgacgc cctaccgcga cgagcatttc ggcaacgtcg
     1981 ccgtgtactt cgagcaggtc gagatcgccg tgccggtgac ccggacggtg gccaccgcgg
     2041 cgaagggcac cctgctgctg ggcctgcagg gctgccagga tggcggcatc tgctatccgc
     2101 cgatgatccg gcggctggcg gtgtcgctgc cggcgggtac cgtcaccccg gttgcgccgg
     2161 atgcgcccgc gctgggcgcc gataccgcac cggtggcatc cgcggctgca gcagccaccg
     2221 gcacggacgc gaccgcaagc cccgctgcga cgggcacgtt cgcggccacc gccggccccg
     2281 acccggtgcg gacgccgccg ccgcagcgcc aggcccatgg cctgctcgct tccctgctgc
     2341 tggcgttgtt cggcggcctg atcctcaacc tgatgccgtg cgtactgccg gtgctgtcgc
     2401 tgaaggcact gtcgctggcg cagagcggcg aaagtcgcga gcgcgcgcgc cggcatgcac
     2461 tcgcctacac cgccggcgtg ctcgccagct tcgccgcgct gggcgcgctg gcgctggcgc
     2521 tgcgcaaggc cgggctggtg ctgggctggg gcttccagct gcagcagccg ctggtggttg
     2581 cgctgctggc gctggtggtg ttcg
//
LOCUS       DAORUK010000213         2600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_58372_length_2600_cov_18.186640, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000213 DAORUK010000000
VERSION     DAORUK010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58372_length_2600_cov_18.186640"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PK818_10385"
     CDS             <1..708
                     /locus_tag="PK818_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014648165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQY83174.1"
                     /translation="SIEGRKNAVDAKRGKIFTKVIREISVAARAGGGDPAGNPRLRSA
                     IDKGLSVNMTKDVIERAIKKATGELEGVEYEEIRYEGYAPGGVAVIVDCLTDNRVRTV
                     ADVRHAFGKFGGNMGNEGSVAFMFRKLGVLSYDAGANEDAITEAAIEAGAEDVTVYDD
                     GAIDVVTAPEEFGTVKAAMEAAGLAPGFAEVTMRADNDIAVEGETAEQVKKLLAWLED
                     MDDVQDVYSNADLGAAA"
     gene            736..1254
                     /gene="ruvC"
                     /locus_tag="PK818_10390"
     CDS             736..1254
                     /gene="ruvC"
                     /locus_tag="PK818_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HQY83175.1"
                     /translation="MTRILGIDPGSQRTGVGIIDVDASGKVTHVHHQPLVLLGAESFP
                     LRLRRLLDGLWALIERHAPDEVAVEQVFLSNNAMSALKLGQARGAAIAACVARDLPVS
                     EYAAKEIKLGLVGSGGADKLQVQHMVGIMLGLQGKLQADAADALAVAITHAHVRATAN
                     RLGVATRTAWRK"
     gene            1274..1864
                     /gene="ruvA"
                     /locus_tag="PK818_10395"
     CDS             1274..1864
                     /gene="ruvA"
                     /locus_tag="PK818_10395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011258432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HQY83176.1"
                     /translation="MIGRLRGILIHKAPPWLVVDVHGVGYELEAPMSTFYDLPELGRE
                     VFLFVHHAQKEDSVSLYGFLREGERRLFRDVQKVSGIGARIALAVLSGVSVEEFARLL
                     HAGDVTALTRIPGIGKKTAERMLVELRDRAVDLAGGGPLAGAAGLPGDPLSEAIDALQ
                     ALGYKPAEAERMARKAIAEGDEAAAIIRKALQSALR"
     gene            1885..>2600
                     /locus_tag="PK818_10400"
     CDS             1885..>2600
                     /locus_tag="PK818_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019186144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HQY83177.1"
                     /translation="MSDKHPGPAQAAQPHGHAQLGLAALVVAAVGVVFGDIGTSPLYT
                     IKEMFNPHFGLTPDPATVKGLLSLGFWSLILVVTLKYVLVIMRADNDGEGGIMALTAL
                     VQRSLKQGSRLSYVVGILGIFGASLFFGDGMITPPITVLGAVEGLAVISPRLADWVVP
                     ISLVILFGLFASQRFGTAKVGRVFGPVMVTWFVVLAALGIYNIIDNPIVLRALSPMWA
                     IGFFAEHGWHAILVLGAVVLT"
BASE COUNT          417 a          862 c          947 g          374 t
ORIGIN      
        1 tccatcgaag gtcgcaagaa cgcggtcgac gccaagcgcg gcaagatctt caccaaggtg
       61 atccgcgaga tcagcgtcgc cgcgcgcgcc ggcggcggcg atcccgccgg caacccgcgc
      121 ctgcgcagcg cgatcgacaa gggcctgtcg gtcaacatga ccaaggacgt gatcgaacgc
      181 gcgatcaaga aggccaccgg cgagctggaa ggcgtcgagt acgaggaaat ccgctacgag
      241 ggctatgccc cgggcggggt ggcggtgatc gtcgactgcc tgaccgacaa ccgcgtgcgc
      301 accgtggccg acgtccgcca cgcgttcggc aagttcggcg gcaacatggg caacgaaggc
      361 tcggtcgcct tcatgttccg caagctcggc gtgctcagct acgacgccgg cgccaacgag
      421 gacgccatca ccgaggccgc gatcgaggcc ggcgccgagg acgtcacggt gtacgacgac
      481 ggtgcgatcg acgtggtcac cgcgccggag gagttcggca cggtcaaggc ggcgatggag
      541 gccgccgggc tggcgccggg cttcgccgag gtgaccatgc gcgccgacaa cgacatcgcg
      601 gtggagggcg agaccgccga gcaggtgaag aagctgctgg cgtggctgga ggacatggac
      661 gacgtccagg acgtctattc gaacgccgac ctgggggccg ccgcgtaagc gcccggcgcg
      721 atgcaggccc gccccatgac gcgcattctc ggcatcgacc ccggttcgca gcggaccggc
      781 gtcggcatca tcgacgtcga cgccagcggc aaggtcaccc atgtgcacca ccagccgctg
      841 gtgctgctcg gcgccgaatc cttcccgctg cgcctgcgca ggctgctgga cgggctgtgg
      901 gcgctgatcg aacggcacgc gccggacgag gtggcggtgg agcaggtgtt cctgtccaac
      961 aacgcgatgt ccgcgctcaa gctggggcag gcgcgcggcg ccgcgatcgc cgcctgcgtg
     1021 gcccgcgacc tgccggtcag cgaatacgcg gcgaaggaaa tcaagcttgg cctggtcggc
     1081 agcggtggcg ccgacaagct gcaggtccag cacatggtcg gcatcatgct gggcctgcag
     1141 ggcaagctgc aggccgacgc cgccgatgcg ctggcggtgg cgatcaccca cgcgcacgtg
     1201 cgcgcgaccg ccaaccggct gggcgtggcc acccgcaccg cgtggaggaa atgacgatgg
     1261 cgggagctgg cgcatgatcg gccgcctcag ggggatcctg atccacaagg cgccgccgtg
     1321 gctggtggtc gacgtgcatg gcgtcggcta cgagctggaa gcgccgatga gcaccttcta
     1381 cgacctgccc gaactcggcc gcgaggtctt cctgttcgtc caccacgcgc agaaggaaga
     1441 cagcgtgtcg ctgtacggct tcctgcggga aggcgagcgg cgcctgttcc gcgacgtgca
     1501 gaaggtcagc gggatcggcg ccaggatcgc gctggcggtg ctgtccgggg tctcggtgga
     1561 ggaattcgcg cggctgctgc acgccggcga cgtgaccgcg ctgacccgca tccccgggat
     1621 cggcaagaag accgccgagc ggatgctggt cgaactgcgc gatcgcgcgg tggacctcgc
     1681 cggcggcggg ccgctggcgg gtgccgcggg actgcccggg gaccccctgt ccgaagccat
     1741 cgacgccctg caggcattgg gctacaagcc ggccgaagcc gaacgcatgg ccaggaaggc
     1801 catcgccgag ggcgacgagg ccgccgccat catccgcaaa gcgctacagt ccgcgctccg
     1861 ctgaggccgg gaacccggaa tcgcatgtca gacaagcatc caggcccggc gcaggccgcg
     1921 caaccgcacg gacatgccca gctcgggctc gcggccctgg tggtggccgc ggtcggcgtg
     1981 gtgttcggcg acatcggcac cagcccgctg tacaccatca aggaaatgtt caacccgcac
     2041 ttcgggttga ccccggaccc ggccaccgtc aagggcctgc tgtcgctggg gttctggtcg
     2101 ctgatcctgg tggtgaccct gaagtacgtg ctggtgatca tgcgcgccga caacgacggc
     2161 gagggcggga tcatggcgct gaccgcactg gtccagcgca gcctgaaaca agggtcgcgg
     2221 ctcagctacg tggtcggcat cctcggcatc ttcggcgcct cgctgttctt cggcgacggc
     2281 atgatcaccc cgccgatcac ggtgctcggc gcggtcgagg ggctggcggt gatctcgccg
     2341 cggctggcgg actgggtggt gccgatcagc ctggtgatcc tgttcgggct gttcgccagc
     2401 cagcgcttcg gcaccgccaa ggtcgggcgg gtgttcggcc cggtgatggt cacctggttc
     2461 gtggtgctgg ccgcgctggg catctacaac atcatcgaca acccgatcgt gctgcgcgcg
     2521 ctgagcccga tgtgggcgat cggcttcttc gccgagcatg gctggcacgc gatcctggtg
     2581 ctgggcgcgg tggtgctgac
//
LOCUS       DAORUK010000214         2591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_58694_length_2591_cov_22.285095, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000214 DAORUK010000000
VERSION     DAORUK010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58694_length_2591_cov_22.285095"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PK818_10405"
     CDS             complement(<1..807)
                     /locus_tag="PK818_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY83178.1"
                     /translation="MNPLPATGARKAALAFIFITVLIDVLAFGVIIPVLPHLVQEFVG
                     GDTSTAAYWTGAFAFSFSLVQFFSAPIQGALSDRYGRRPVILVSCLGLGLDFIFMALA
                     PNLAWLFVGRIISAATSASFTTANAYIADVTAPEARAKSFGMIGAAFGLGFIVGPLLG
                     GVLGDIDHRLPFWFSACLALLNFGYGLFVLPESLPKEKRAPVFDWQHARPMGGVNMLR
                     QYPQIWGLVAVVFVANFAHYVYPSTFVLFADASYGWKEKQAGYVLSVVGVL"
     gene            complement(826..1410)
                     /locus_tag="PK818_10410"
     CDS             complement(826..1410)
                     /locus_tag="PK818_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2239 family protein"
                     /protein_id="HQY83179.1"
                     /translation="MIHPTLRCTAFAGPRRIASGELRHVALKAKQAFDAGKPVRVFED
                     ASGMPVALPLALPAGELLRWLADPLATAVQDAQAAPRGPGRPKLGVVAREVTLLPGHW
                     DWLAAQPGGASVALRALVDAARHVAPEGDRRRAAQEAGYRFMQAMAGDQAGFAGACRA
                     LFAGDIGAFEEHCATWPDDVREHAGLLAADAFHH"
     gene            complement(1472..2083)
                     /gene="folE"
                     /locus_tag="PK818_10415"
     CDS             complement(1472..2083)
                     /gene="folE"
                     /locus_tag="PK818_10415"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HQY83180.1"
                     /translation="MSDHDSADDRRNDVSRAQAEEAVRTLLRWSGDDPAREGLLDTPR
                     RVAKAYQDWFSGYALDPDEYLQRTFEEVGGYDEMIVLRDIDYESHCEHHMAPIIGKVH
                     IGYLPNGKVVGISKLARVVEAYARRFQVQEKMTAQIANCIQRALQPRGVGVVVEGAHE
                     CMTTRGIHKRGVSMVTSKMLGSFREDARTRAEFLQFIDVGQGR"
     gene            complement(2189..2410)
                     /locus_tag="PK818_10420"
     CDS             complement(2189..2410)
                     /locus_tag="PK818_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83181.1"
                     /translation="MNTLVALLAWCVLLVLCWPLALLALVLWPIAWLLSLPLRLVGIT
                     FSALFAFLHALLLLPARVLGWRPRAVAVA"
BASE COUNT          447 a          934 c          876 g          334 t
ORIGIN      
        1 gagcacgccg accaccgaca gcacgtagcc ggcctgcttt tccttccagc cgtagctggc
       61 gtcggcgaac agcacgaagg tgctgggata cacgtagtgg gcgaagttgg cgacgaacac
      121 caccgccacc agcccccaga tctgcggata ctggcgcagc atgttgaccc cgcccatcgg
      181 ccgggcgtgc tgccagtcga acaccggcgc gcgcttctcc ttcggcagcg attccggcag
      241 cacgaacagg ccgtagccga agttgagcag ggccaggcag gccgagaacc agaacggcag
      301 gcgatggtcg atgtcgccga gcacgccgcc cagcaacggg ccgacgatga agcccaggcc
      361 gaacgccgcg ccgatcatgc caaagctctt cgcgcgcgcc tccggcgcgg tgacgtcggc
      421 gatgtaggcg ttggcggtgg tgaaactggc agaggtcgcc gccgagatga tccggcccac
      481 gaacagccag gccaggttcg gcgccagcgc catgaagatg aagtccaggc ccagcccgag
      541 gcaggacacc aggatcaccg gccgccgccc gtaacggtcc gacagcgcgc cctggatcgg
      601 tgcgctgaag aactgcacca gcgagaacga gaacgcgaac gcaccggtcc agtacgcggc
      661 ggtggaggtg tcgccgccga cgaattcctg caccaggtgc ggcagcaccg ggatgatcac
      721 cccgaacgcc agcacgtcga tcagcacggt gatgaagatg aaggccagcg cagccttgcg
      781 ggcgccggtg gcgggcagtg ggttcaaggg gtgtcctcgc ggccgtcagt ggtggaacgc
      841 gtcagccgcc agcagcccgg catgttcgcg cacatcgtca ggccatgtcg cgcaatgttc
      901 ctcgaaggcg ccgatgtcgc cggcgaacag cgcgcggcag gcaccggcga agccggcctg
      961 gtcgccggcc atggcctgca tgaagcggta gccggcttcc tgcgcggcgc ggcggcggtc
     1021 gccttcaggc gcgacatggc gcgcggcgtc gaccagtgcg cgcaatgcca ccgacgcgcc
     1081 gcccggctgc gccgccagcc agtcccaatg cccgggcagc agggtgacct cgcgcgccac
     1141 cacccccagc ttcggccgcc cgggcccgcg cggcgccgct tgcgcgtcct gcacggcggt
     1201 ggccagcgga tccgccagcc accgcagcaa ctcgccggcg ggcagcgcca gcggcagcgc
     1261 gaccggcatc ccgctggcgt cctcgaacac ccgcaccggc ttgcccgcat cgaacgcctg
     1321 cttggccttc agcgcaacgt ggcgcaattc gccgctggcg atccggcgcg ggccggcgaa
     1381 cgccgtgcag cgcaaggtgg gatggatcat cgaacgcgcc ttccgggaca ggcgtggatt
     1441 ctgcccggat caaactcgac ggacaaccgg ctcagcgccc ctgcccgaca tcgatgaact
     1501 gcaggaattc ggcgcgggtg cgcgcgtcct cgcggaagct gcccagcatc ttcgaggtga
     1561 ccatgctcac cccgcgcttg tggatgccgc gcgtggtcat gcactcgtgc gcgccctcca
     1621 ccaccacgcc gacaccgcgc ggttgcagcg cgcgctggat gcagttggcg atctgcgcgg
     1681 tcatcttctc ctgcacctgg aagcggcgcg cgtaggcctc gaccacccgc gccagcttgc
     1741 tgatgccgac caccttgccg ttcggcaggt agccgatgtg gaccttgccg atgatcggcg
     1801 ccatgtggtg ttcgcaatgg ctttcgtaat cgatgtcgcg cagcacgatc atctcgtcgt
     1861 agccgcccac ttcctcgaag gtgcgctgca ggtattcgtc cggatccagc gcatagccgc
     1921 tgaaccagtc ctggtaggcc ttggccacgc gcctgggggt gtccagcagg ccttcgcgcg
     1981 cggggtcgtc gccggaccag cgcaacaggg tgcgcacggc ctcctcggcc tgcgcgcggc
     2041 tgacgtcgtt gcgccggtcg tcggcggagt cgtggtctga catgcgtggg tccctcgcga
     2101 aggggtgcat cgtaccgcgc ccggcaatcc gggccgcgtc gatccggttc ctacgcaacc
     2161 cggcgaaaaa ccgatgcagc agccggcatc aggcgaccgc cacggcccgc gggcgccacc
     2221 ccaacacgcg tgccggcaac agcagcagcg catgcaggaa ggcgaacagc gccgagaagg
     2281 tgatgccgac cagccgcagc ggcagcgaca gcagccaggc gatcggccac agcaccagcg
     2341 ccagcaacgc cagcggccag cacagcacca gcagcacgca ccaagcaagc agggcgacca
     2401 gggtgttcat cgttcgggcc cgcgatggca acgctgcaca cgcagcgcct gccacatcgc
     2461 catgcgcccg ccgtgcacgc aagcggcgtg cgacgaaacg cgggagggcg cgacgaacgc
     2521 cacccccgcg ccttcggtca gaacgcgttg tcgccgtcga ggatgcggcc gatcccgccc
     2581 agcaccgagc c
//
LOCUS       DAORUK010000215         2571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_59465_length_2571_cov_18.357711, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000215 DAORUK010000000
VERSION     DAORUK010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59465_length_2571_cov_18.357711"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="PK818_10425"
     CDS             <1..1136
                     /locus_tag="PK818_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HQY83182.1"
                     /translation="RPHAVPQAHGRERPFAAEVLANQRITGRGSARDIRHLELSLEGS
                     GLAYEPGDALGVWPRNPPVLVDALLAALDFDGDAVVAHTGTELPLREWLSSKREITRL
                     SRPFIARHADLAADDALTRLLSPGNAAQLASLLGSRQLIDLLQAAPARWSADEFVAAL
                     RPLQPRLYSIASSRKAVGEEAHLAVAHVEYDNGDGLRWGAASHFLASRGDGDRADVFI
                     EANERFRLPADGARDIVMIGPGTGVAPFRGFVQERRETAASGRNWLLFGNSHARSEFL
                     YQTEWQQALKDGALHRLDLAFSRDQAEKIYVQQRLREHGRELYAWLENGAHVYVCGAL
                     AMGKDVHAALLEVVARERGVDADAAADYVAALQAQGRYARDVY"
     gene            1136..>2571
                     /locus_tag="PK818_10430"
     CDS             1136..>2571
                     /locus_tag="PK818_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004089014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HQY83183.1"
                     /translation="MSAHSVEHIKQDSARLRGTLLESLANPITGALADGDQTLIKYHG
                     SYQQDDRDIREERRRQKLEPDYSFMIRTRTPGGVVTPAQWLKLDAIATTYAERGLRIT
                     TRQAFQFHGVIKTRLKPTMQAINAALIDTLAACGDVNRNVVVAANPLLSPVHATVHAQ
                     AAALSERLLPNTRAYYEIWLDEEKVAGSGEETEPVYGETYLPRKFKIAFAIPPTNDAD
                     VFANDLGLIAIIEGDEVVGYNVSVGGGMGATHGDAETYPRLADVIGFIAPEQLFDVAL
                     AVITTQRDFGNRAVRKRARLKYTIDDRGIDWFKDEVERRAGFPLGPARPFAFDHNGDR
                     FGWREGSDGHCHLTLRIPAGRIVDNDDVKHLSGLREIAKVHEGEFRMTPNQNLVIAGV
                     PAGQRERIDALVREYALDAHVGASGLRLNALACVSLPTCGLAMAEAERYLPELLDKAE
                     TLLARHDLFDAPIHLRISGCPNGCSRPYL"
BASE COUNT          427 a          923 c          858 g          363 t
ORIGIN      
        1 tgcgcccgca cgcggtgccg caggcgcatg gccgcgagcg tccgttcgcc gcggaagtgc
       61 tggccaacca gcgcatcacc ggccgtggca gcgcgcgcga catccgccac ctggaactgt
      121 cgctggaagg ctcgggcctg gcctacgaac ccggcgacgc gctgggcgtg tggccgcgca
      181 atccaccggt gctggtcgat gccttgctgg ctgcactcga cttcgatggc gatgccgtgg
      241 tcgcgcacac cggcaccgaa ctgcccttgc gcgaatggct gtcgtcgaag cgcgagatca
      301 cccggctgtc gcgcccgttc atcgcccgcc atgccgatct ggccgcggac gatgcgctca
      361 cccgcctgct gtcgcccggc aacgccgcgc aactggccag cctgctcggc agccgccagc
      421 tgatcgacct gctgcaggcg gcaccggcac ggtggtccgc ggacgagttc gtcgccgcct
      481 tgcgcccgtt gcagccgcgg ctgtactcca tcgcgtccag ccgcaaggcc gtgggcgagg
      541 aagcacacct cgccgtcgcc catgtcgaat acgacaacgg cgatggcctg cgctggggcg
      601 cggcctcgca tttcctcgcc agccgcgggg acggcgaccg tgccgacgtc ttcatcgaag
      661 cgaacgagcg cttccgcctg cccgccgatg gcgcgcgcga catcgtgatg atcggccccg
      721 gcaccggcgt ggcgccgttc cgcggcttcg tgcaggagcg ccgcgagacc gccgccagcg
      781 gccgcaactg gctgctgttc ggcaactcgc atgcacgcag cgaattcctg taccagaccg
      841 aatggcagca ggcgttgaag gatggcgcgc tgcaccggct ggacctggcg ttctcgcgcg
      901 accaggccga gaagatctac gtgcagcagc gcctgcgcga acacggccgc gagctgtacg
      961 cgtggctgga aaacggcgcg cacgtgtatg tctgcggcgc gctggcgatg ggcaaggacg
     1021 tgcatgccgc gctgcttgag gtggtcgcgc gcgaacgtgg cgtggatgcc gacgccgccg
     1081 ccgactacgt cgccgcgctg caggcgcagg gccgctacgc acgggatgtc tattgatgag
     1141 cgcgcactcg gtcgaacaca tcaagcagga cagcgcgcgc ctgcgcggca ccttgctgga
     1201 atcgctggcc aacccgatca ccggcgcact ggccgacggc gaccagaccc tgatcaagta
     1261 ccacggcagc taccagcagg acgaccgcga catccgcgag gagcgccgca ggcagaagct
     1321 ggagcccgac tacagtttca tgatccgcac ccgcacgccc ggcggcgtgg tcacgccggc
     1381 gcagtggctg aagctggacg cgatcgcgac cacctacgcc gagcgcggcc tgcgcatcac
     1441 cacccgccag gccttccagt tccacggcgt gatcaagacc cggctgaagc cgaccatgca
     1501 ggcgatcaac gccgcgctga tagacaccct ggccgcctgc ggcgacgtca accgcaatgt
     1561 cgtggttgcc gccaacccgc tgctgtcgcc ggtgcacgcc acggtgcacg cgcaggccgc
     1621 ggccctgagc gagcgcctgc tgccgaacac gcgcgcctac tacgagatct ggctggacga
     1681 ggagaaggtc gcgggcagcg gcgaggaaac cgagccggtg tacggcgaga cctacctgcc
     1741 gcgcaagttc aagatcgcct tcgcgatccc gccgaccaac gacgccgacg tgttcgccaa
     1801 cgaccttggc ctcatcgcca tcatcgaagg cgatgaagtg gtcggctaca acgtcagcgt
     1861 tggcggcggc atgggcgcga cccacggcga tgccgagacc tatccgcgcc tggccgacgt
     1921 gatcggcttc atcgcgcccg agcagttgtt cgatgtcgcc ctcgccgtca tcaccacgca
     1981 gcgcgacttc ggcaaccgcg cggtgcgcaa gcgcgcacgc ctgaagtaca ccatcgacga
     2041 ccgcggcatc gactggttca aggacgaagt ggagcggcgc gccggctttc cccttgggcc
     2101 ggcgcggccg ttcgccttcg accacaacgg cgaccgcttc ggctggcgcg aaggcagcga
     2161 tggccactgc cacctgaccc tgcggattcc cgccgggcgc atcgtcgaca acgacgacgt
     2221 caaacacctc tcgggcctgc gcgaaatcgc caaggtgcac gaaggcgaat tccggatgac
     2281 gccgaaccag aacctggtga tcgccggcgt gcctgcgggc caacgcgagc gcatcgatgc
     2341 attggtgcgc gagtacgcac tggatgcgca tgtgggcgcc agcgggctgc gcctcaatgc
     2401 actggcctgc gtgtcgctgc cgacctgcgg cctggcgatg gccgaagccg aacgctacct
     2461 gcccgagttg ctcgacaagg cggaaacgct gctggcgagg cacgacctgt tcgacgcacc
     2521 gatccacctg cgcatcagtg gctgcccgaa cggctgctcg cggccgtacc t
//
LOCUS       DAORUK010000216         2554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_60161_length_2554_cov_21.738295, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000216 DAORUK010000000
VERSION     DAORUK010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60161_length_2554_cov_21.738295"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2347)
                     /locus_tag="PK818_10435"
     CDS             complement(<1..2347)
                     /locus_tag="PK818_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012438089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HQY83184.1"
                     /translation="MTSKATSSENPSVKSILAALAKRVPKSRHAEAQGFANAFYQRMG
                     SDDAVQHGTDGWAALAADMLEFARTRKRGRANVRLFNPTLKEHGWESAHTVVQVVNDD
                     MPFLVDSVTMALSERGIGVHVLGHPVMPITRDKAGKLAAVGEGALESFIHLEVDRQPQ
                     DVMAAIKAGIEEVLADVRAMVADWEPMHARMLAVADEITERRMPVSDEGKREAQEFLR
                     WVASDHFTFLGYREYKVVKQGKAEVLVADEDSGLGLLRGKDAGKPRPLTSLAAHYMPH
                     SGSVDALILTKTNARATVHRPGYMDYIGVLSFDKSGKPVAEQRFLGLYTSSAYHRRPW
                     DIPLVRERFEHVMRQSGLNPNSHSGKALRHILETLPRDELFQSSEQELLRTSLGILGL
                     QERVRSKLFLRRDRFGRYFSALVYVPRDRMSTQLRHRIEDMLKQALEGEHIDTTVQIG
                     ESPLAQMHMIVRPRAGDAVEVDQAALEQKLGEIVRDWRDELREQLVARHGEEAGLKLA
                     GRFGRALPAGYIEEVSPEVAAADVEHLAALAGPEDLRLSLYRSRRKHGGLRFKLYRQQ
                     DDIPLSDALPMMENMGLRVISEHPYRVDASDGLCYIQDFEVESLQGELDVDALDENFE
                     ESFARLWRGDAENDGFNRLVLAAGLSWRQVAMLRSYCKYLLQVGVPFSQSYVEATLAR
                     YPLLSRLLVELFEARFDPSTGKESAAEIRRGMDRFGAQLKALAAGDEGAMAALQQLIA
                     ARSGSRERQVEAARAALLGLMDRVSSLDEDRILRSFIGVIDA"
     gene            complement(2447..>2554)
                     /locus_tag="PK818_10440"
     CDS             complement(2447..>2554)
                     /locus_tag="PK818_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQY83185.1"
                     /translation="MPLERYFRDAKITEIYEGTSEIQRLVIARNETGLR"
BASE COUNT          348 a          895 c          872 g          439 t
ORIGIN      
        1 tcgcgtcgat caccccgatg aagctgcgca ggatgcggtc ctcgtcgagg ctggagacgc
       61 ggtccatcag gcccagcagg gccgcgcgcg ccgcctcgac ctggcgctcg cggctgccgc
      121 tgcgggccgc gatgagctgc tgcagggccg ccatcgcgcc ctcgtcgccg gcggccagcg
      181 ccttcagctg ggcgccgaag cggtccatcc cgcgcctgat ctcggccgcg ctctccttgc
      241 cggtgctggg gtcgaagcgc gcctcgaaca gttccaccag cagccgcgac agcagcggat
      301 agcgcgccag ggtggcctcg acgtagctct gcgagaacgg caccccgacc tgcagcaggt
      361 acttgcagta gctgcgcagc atcgccacct gccgccacga caggcccgcc gccagcacca
      421 gccggttgaa gccgtcgttc tcggcatcgc cgcgccagag ccgggcgaag gattcctcga
      481 agttctcgtc gagcgcgtcg acgtcgagtt caccctgcag cgactcgacc tcgaagtcct
      541 ggatgtagca caggccgtcg ctggcatcga cccggtacgg atgctcggag atcacccgca
      601 ggcccatgtt ctccatcatc ggcagcgcgt cggacagggg gatgtcgtcc tgctggcggt
      661 acagcttgaa gcgcagcccg ccgtgcttgc ggcgcgagcg gtacagcgac agccgcaggt
      721 cctcgggccc ggccagcgcg gccaggtgct cgacgtcggc ggcggcgacc tcggggctga
      781 cttcctcgat gtagcccgcc ggcaacgcgc ggccgaagcg gcccgccagc ttcagccctg
      841 cttcctcgcc atgccgcgcc accagttgct cgcgcagttc gtcgcgccag tcgcggacga
      901 tctcgcccag cttctgctcc agcgcggcct ggtcgacctc gaccgcgtcg ccggcccgcg
      961 gccgcacgat catgtgcatc tgcgccagcg gcgattcgcc gatctgcacg gtggtgtcga
     1021 tgtgctcgcc ttcgagcgcc tgcttgagca tgtcctcgat gcggtggcgc agctgggtgc
     1081 tcatgcggtc gcgcgggacg tacaccagcg ccgagaaata acgcccgaag cggtcgcggc
     1141 gcaggaacag cttgctgcgc acgcgctcct gcaggccaag gatgccgagg ctggtgcgca
     1201 gcagctcctg ctcgctcgac tggaacagtt cgtcgcgcgg cagggtttcc aggatgtggc
     1261 gcagggcctt gccgctgtgg ctgttggggt tgagcccgga ctggcgcatc acgtgctcga
     1321 agcgctcgcg caccagcggg atgtcccacg ggcggcggtg gtaggcgctg gaggtgtaca
     1381 ggccgaggaa gcgctgctcg gccaccggct tgccggactt gtcgaagctg agcacgccga
     1441 tgtagtccat gtagccgggg cgatggacgg tggcgcgcgc gttggtcttg gtcaggatca
     1501 gcgcatccac cgaaccgctg tgcggcatgt agtgcgcggc cagcgaggtc agcggccgcg
     1561 gcttgccggc gtccttgccg cgcagcaggc cgagcccgct gtcctcgtcg gccaccagca
     1621 cttccgcctt gccctgcttg accaccttgt actcgcggta gccgaggaag gtgaaatggt
     1681 cgctggcgac ccagcgcagg aattcctgcg cctcgcgctt gccctcgtcg ctgaccggca
     1741 tccggcgctc ggtgatctcg tccgcgaccg ccagcatcct cgcgtgcatc ggctcccagt
     1801 cggccaccat cgcgcgcacg tccgcgagga cctcctcgat cccggccttg atcgccgcca
     1861 tcacgtcctg cggctggcga tcgacttcca ggtggatgaa ggattccagc gcgccctcgc
     1921 ccaccgccgc cagcttgccg gccttgtcgc gggtgatcgg catcaccgga tgcccgagca
     1981 cgtgcacgcc gatgccgcgc tcggacaatg ccatggtcac cgagtccacc aggaacggca
     2041 tgtcgtcgtt caccacctgc accaccgtgt gcgccgactc ccagccgtgt tccttcaggg
     2101 tcgggttgaa caggcggacg ttggccctgc cacgcttgcg ggtcctggcg aactccagca
     2161 tgtcggccgc cagtgcagcc cagccgtcgg tgccgtgctg gaccgcatca tcgctgccca
     2221 tgcgctggta gaaggcattg gcgaagccct gggcctcggc gtgcctggac ttgggcacgc
     2281 gcttggcgag tgcggccagg atcgatttga cggacgggtt ttcgctgctg gtggctttgc
     2341 tggtcatcgc gggattgcct tggatggatg cttgccgcgc atgcgcggcg tcggttgcct
     2401 ttgcgcatgg ccgcgcaccc gcggccatgg cgtcgggttg gtcgggtcag cgcaggccgg
     2461 tctcgttgcg cgcgatcacc aggcgctgga tctcgctggt gccttcgtag atctcggtga
     2521 tcttggcgtc gcggaaatag cgctccagcg gcat
//
LOCUS       DAORUK010000217         2530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_61072_length_2530_cov_20.472323, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000217 DAORUK010000000
VERSION     DAORUK010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61072_length_2530_cov_20.472323"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PK818_10445"
     CDS             <1..578
                     /locus_tag="PK818_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HQY83186.1"
                     /translation="AMSIGLLVDQDTPMIWRGPMATSALTQLLTGTRWGDLDVLVVDL
                     PPGTGDIQLTMSQKIPVAGAVIVTTPQDIATLDARKALKMFEKLDIAVLGLVENMAVH
                     VCSNCGHAEHIFGEGGAVRMSAQYGVPVLGSLPLQVAIRAQGDAGTPIVAAQPDSAAA
                     LAYREAARALLARLDTRPKVRSGIMASLLGG"
     gene            633..1220
                     /gene="dcd"
                     /locus_tag="PK818_10450"
     CDS             633..1220
                     /gene="dcd"
                     /locus_tag="PK818_10450"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005912996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HQY83187.1"
                     /translation="MSIKSDRWIRRMAQEHGMLEPFEPGQVRFADNGGVDGRRIVSYG
                     TSSYGYDVRCSREFKVFTNINSTIVDPKRFDPKSFVDVVADECIIPPNSFALARTVEY
                     FRIPRDTLVVCLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPARIYANEGVA
                     QMLFFQADADDVCETSYKDRGGKYQGQTGVTLPKT"
     gene            complement(1224..1646)
                     /locus_tag="PK818_10455"
     CDS             complement(1224..1646)
                     /locus_tag="PK818_10455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HQY83188.1"
                     /translation="MPHAATRFALHPQLADDTAPVIELPLCEVRLMDDANHPWLVLVP
                     RVADAVELIDLDATQRAQLSTEIDAAARALKALFKPHKLNIAALGNLVPQLHVHVIAR
                     YTDDIAWPRPVWGAANARPYAPEDLIERVNALRAALAG"
     gene            complement(1666..2334)
                     /locus_tag="PK818_10460"
     CDS             complement(1666..2334)
                     /locus_tag="PK818_10460"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQY83189.1"
                     /translation="MSEWTSLETPTGRIRAWRADPHTPPRGALVVVQEIFGVNAHIRE
                     LCKTFADHGYVAMAPSFFDHFEHDVQLRYDPDGVARGLELVERLGFERALEDVHATAT
                     VLAEHGKVGVVGYCWGGTVAFLANTRLSMPAVSYYGARTVPFLAERIEAPMMFHFGER
                     DPSITAHDIELHREHQEHAEVHVYPAGHGFNCDQRADFERHSAALALEHTLAFFRRTL
                     HPAY"
BASE COUNT          393 a          858 c          887 g          392 t
ORIGIN      
        1 aggcgatgtc gatcggcctg ctggtggacc aggacacgcc gatgatctgg cgcggaccga
       61 tggcgacctc ggcgctgacc cagttgctga ccgggacccg ctggggcgac ctcgacgtgc
      121 tggtggtgga cctgccgccg ggcaccggcg acatccagct gaccatgtcg cagaagatcc
      181 cggtggcggg cgcggtgatc gtgaccacgc cgcaggacat cgccaccctg gacgcgcgca
      241 aggcgctgaa gatgttcgag aagctcgaca tcgcggtgct cggcctggtc gagaacatgg
      301 ccgtgcacgt gtgttcgaac tgcggccatg ccgagcacat cttcggcgag ggcggggcgg
      361 tgcggatgtc cgcgcaatac ggggtaccgg tgctgggcag cctgccgctg caggtcgcga
      421 tccgcgcaca gggcgatgcc ggcacgccga tcgtggcggc gcagccggac tcggccgccg
      481 cgctggccta ccgcgaggcc gcccgcgcgc tgctggcgcg gctggacacc cgcccgaagg
      541 tgcgctccgg gatcatggcc tcgttgcttg gtggctgatc ggacggggtc gaccccttag
      601 aatcgccggc ttcgccccaa gaaagacagc agatgagcat caagagcgac cgctggatcc
      661 gccgcatggc gcaggagcac ggcatgctgg agccgttcga gcccgggcag gtccggtttg
      721 ccgataacgg cggggtcgac ggtcgccgca tcgtcagcta tggcacctcc agctacggct
      781 acgacgtgcg ctgctcgcgc gagttcaagg tattcaccaa catcaactcg accatcgtcg
      841 atcccaagcg cttcgacccg aagagcttcg tcgacgtggt cgcggacgaa tgcatcatcc
      901 cgcccaattc gttcgcgctg gcgcgcacgg tggagtactt ccggatcccg cgcgacaccc
      961 tggtggtgtg cctgggcaag agcacctacg cgcgctgcgg gatcatcgtc aacgtgaccc
     1021 cgctggagcc ggagtgggag ggccacgtca ccctggaatt cagcaacacc acgccgctgc
     1081 cggcgcgcat ctacgccaac gagggcgtgg cgcagatgct gttcttccag gccgatgccg
     1141 acgacgtctg cgagaccagc tacaaggatc gtggcggcaa gtaccagggc cagaccggcg
     1201 tcaccctgcc gaagacctga gtcctacccg gcgagcgctg cgcgcagcgc gttgacccgc
     1261 tcgatcaggt cttccggcgc atacggccgg gcattcgccg caccccacac cgggcgcggc
     1321 caggcgatgt cgtcggtgta acgcgcgatc acgtgcacgt gcaactgcgg caccaggttg
     1381 ccgagcgcgg cgatgttgag cttgtgcggc ttgaacaggg ccttgagcgc gcgcgcggcg
     1441 gcatcgatct cggtgctgag ctgggcgcgc tgggtggcat cgaggtcgat cagttcgacc
     1501 gcatccgcca cccgcggcac cagcaccagc cacgggtggt tggcatcgtc catcagccgc
     1561 acttcgcaca gcggcagctc gatcaccggc gcagtgtcgt cggccagctg cgggtgcagg
     1621 gcgaaccggg tggcggcgtg cggcatgcgt ggcatctcgg cgggctcaat aggccgggtg
     1681 cagggtccgg cggaagaatg cgagggtgtg ctccagcgca agggcggcgc tgtggcgttc
     1741 gaagtcggcg cgctggtcgc agttgaagcc gtgcccggcc gggtacacgt gcacctcggc
     1801 gtgttcctgg tgctcgcggt gcagttcgat gtcgtgggcg gtgatcgagg ggtcgcgttc
     1861 gccgaagtgg aacatcatcg gcgcctcgat ccgctcggcg aggaagggca cggtgcgcgc
     1921 accgtaatag ctcaccgccg gcatcgacag ccgggtgttg gcgaggaagg cgacggtgcc
     1981 gccccagcaa tagccgacca ccccgacctt gccatgctcc gcgagcaccg tcgcggtggc
     2041 gtgcacgtcc tccagcgcac gctcgaagcc caggcgctcc accagctcca gcccgcgggc
     2101 gacgccgtcg gggtcgtagc gcagttgcac gtcgtgttcg aaatggtcga agaacgacgg
     2161 cgccatcgcc acgtagccgt ggtcggcgaa ggtcttgcag agctcgcgga tgtgcgcatt
     2221 gacgccgaag atttcctgca ccaccaccag cgcgccgcgc ggcggggtat gcgggtccgc
     2281 ccgccaggcg cggatgcggc cggtcggggt ctcgagggat gtccactcgc tcatgcgccg
     2341 cactccttcc gtctgccgcc gatggtacgc ctccgcgggc cgtccggcag gccaacggta
     2401 tacttcgcgc cccgtggcgg ccggtccgcc cccctgtcgc gtcccgccat gtccctgcaa
     2461 caacccaagg tcggcttcgt cagcctcggc tgccccaagg cgctggtcga ttccgagcgc
     2521 atcctcaccc
//
LOCUS       DAORUK010000218         2520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_61486_length_2520_cov_24.754564, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000218 DAORUK010000000
VERSION     DAORUK010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61486_length_2520_cov_24.754564"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="PK818_10465"
     CDS             complement(<1..1261)
                     /locus_tag="PK818_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQY83190.1"
                     /translation="MSGGGSLSTWGIRNPVPAMMLFFVLCVAGLWGFHQLPVARFPDI
                     AFPMTTVTITQPGASPSQLETEVTRKVEDSVATLDNVKRVTSTVVEGTSTTAIEFNLE
                     ADIMTALNDTKDAVTRIRMNLPQDIQEPIIAKVEIGGSLLTYAVSAPGMAPDELSWFV
                     DRHVMRALYGVEGVAAINRIGGVERQVRVDLDPQALQALGVTASEISQQLAATQVERP
                     GGKAELDGGQQTVRTIGTIADAQALRDYSIALGNGRDVRLSALATITDGAADPTQLAL
                     LDGKPVVGFSLARSRGADELKVRDGIKAALAQLAKDHPGTSYRLVTDMSEETERSYSS
                     SMTMLWEGALLALAVVFWFLRDWRATWVSAIALPLSIIPTFAVMQWFGFSLNIITLLA
                     LSVVVGILVDDAIVEIENIVRHLGMGKK"
     gene            complement(1258..2361)
                     /locus_tag="PK818_10470"
     CDS             complement(1258..2361)
                     /locus_tag="PK818_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQY83191.1"
                     /translation="MRRTTRNFSLGLALCVATIATACSKQDEEAAKPVAAFAVSVVRV
                     QARDIPRSVLVSGPVSAWEEMQLGVEVSGLRVNHLLVDVGQQVRKGDLLLQLDHRSLD
                     ADLAQADASLREAEAGAALARSQLARGELLVKDKYISATQVDELRAARMQGDARVGTA
                     RAMRDTATLRRSFADLRAPDAGVISRRLVQPGQVVAAGSELLRLIRQGRLEWRAELAE
                     AELARVTPGDRIELTTRDGTAVIGQVRAVSPGVDASTRTGTVYADLPDPQGLQPGTYL
                     QGRIDTGIGQGLTVPAATVVQRDGHPNVFAVDAKGIARRVRIRTGGIANGQVEVLEGL
                     KAGDAVVEQGAGFLADGDSVRIVEATATPAPTP"
BASE COUNT          373 a          880 c          871 g          396 t
ORIGIN      
        1 gtttcttgcc catgccgagg tggcggacga tgttctcgat ctcgacgatc gcgtcatcca
       61 ccaggatgcc gaccaccacg ctcagcgcca gcaacgtgat gatgttgagg ctgaaaccga
      121 accactgcat caccgcgaag gtcgggatga tcgacagcgg caaggcgatc gcgctgaccc
      181 aggtcgcgcg ccagtcgcgc aggaaccaga acaccaccgc cagcgccagc agcgcgcctt
      241 cccacagcat cgtcatcgac gacgaatacg aacgctcggt ctcctccgac atgtcggtga
      301 ccagccggta gctggtgccc ggatggtcct tcgccagctg cgccagcgcc gccttgatgc
      361 cgtcgcgcac cttgagttcg tcggcgccgc ggctgcgcgc cagcgagaac ccgaccaccg
      421 gcttgccgtc cagcaaggcg agctgggtgg gatcggcggc gccgtcggtg atcgtggcca
      481 acgcggacaa ccgcacgtcc cggccattgc cgagggcgat gctgtaatcg cgcagcgcct
      541 gcgcatcggc gatcgtgccg atcgtgcgca cggtctgctg gccaccgtcg agttcggcct
      601 tgccgcccgg gcgctccacc tgcgtcgcgg ccagttgctg gctgatctcg ctggcggtga
      661 cgccaagcgc ctgcagcgcc tgcggatcca ggtccacccg cacctgccgc tcgacgccgc
      721 cgatgcggtt gatcgcggcc acgccttcca cgccgtacag cgcgcgcatc acgtggcgat
      781 cgacgaacca cgacagttcg tccggcgcca tgcccggcgc actcaccgca taggtcagca
      841 gcgaaccgcc gatctcgacc ttggcgatga tcggttcctg gatgtcctgc ggcaggttca
      901 tccggatccg cgtcaccgcg tccttggtgt cgttcagcgc ggtcatgatg tccgcctcga
      961 ggttgaactc gatggcggtg gtcgaggtgc cttcgaccac ggtggaggtg acgcgcttga
     1021 cgttgtcgag cgtcgccacc gagtcctcga ccttgcgggt gacctcggtc tccagctgcg
     1081 atggcgaggc gcccggctgg gtgatggtga ccgtggtcat cgggaacgcg atgtccggga
     1141 agcgtgcgac cggcagctgg tggaagcccc acaggccggc cacgcacagg acgaagaaca
     1201 gcatcatcgc ggggaccggg ttgcggatcc cccaggtgga caggctgccg ccgccgctca
     1261 tggcgtgggg gccggggtgg ccgtggcctc gacgatgcgc acgctgtcgc cgtcggcgag
     1321 gaagccggcg ccctgctcga ccaccgcatc gcctgccttc agtccttcca gcacttcgac
     1381 ctggccattg gcgatgccac cggtgcggat gcgcacgcgg cgggcgatgc ccttggcgtc
     1441 gacggcgaac acattcggat ggccgtcgcg ctggaccacg gtggcggcgg gcacggtcag
     1501 gccctggccg atgccggtgt cgatgcgccc ttgcaggtag gtgccgggtt gcaggccctg
     1561 cggatccggc aggtcggcat acacggtgcc ggtgcgggtg ctcgcatcga cgccggggct
     1621 gaccgcgcgc acctggccga tgaccgcggt gccgtcgcgg gtcgtcagtt cgatgcggtc
     1681 gccgggcgtg acccgggcga gttcggcttc ggccagttcg gcgcgccact ccagccggcc
     1741 ctggcggatc agccgcagca gttcgctgcc ggcggcaacc acctggccgg gctgcaccag
     1801 ccgcctggag atcacccctg catcgggggc acgcaggtcg gcgaagctgc ggcgcagggt
     1861 cgcggtatcg cgcattgccc gtgcggtgcc gacgcgcgca tcgccctgca tccgcgccgc
     1921 gcgcaattca tccacctggg tcgcgctgat gtacttgtcc ttcaccagca gttcgccgcg
     1981 cgccagctgg gaacgcgcca gcgccgcgcc ggcctccgcc tcgcgcagcg atgcgtcggc
     2041 ctgtgcaagg tcggcatcca ggctgcgatg gtcgagttgc agcagcaggt cgcccttgcg
     2101 cacctgctgg ccgacgtcaa ccagcaggtg gttgaccctc aagccgctga cttcgacgcc
     2161 gagctgcatt tcttcccacg ccgacaccgg cccggacacc agcacgctgc gcgggatgtc
     2221 ccgcgcctgc acgcgcacca cgctgaccgc gaaggccgca accggcttgg cggcctcttc
     2281 atcctgcttg ctgcaggcgg tggcgatggt ggccacgcaa agcgcgagtc cgagggaaaa
     2341 gttgcgagtg gtacggcgca tgcgcaggtc cgtggacggc aggcgcgcag tctagcgcgt
     2401 atgtgaaccg atcagttcac taactggcgg gcacatggga cgaacgcatg gcccgctcag
     2461 gcgggcggca aacggaagaa cccggcgctg gtcgccacgg tggcggcggc cgtgctggcc
//
LOCUS       DAORUK010000219         2512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_61797_length_2512_cov_15.420431, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000219 DAORUK010000000
VERSION     DAORUK010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61797_length_2512_cov_15.420431"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PK818_10475"
     CDS             complement(<1..313)
                     /locus_tag="PK818_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83192.1"
                     /translation="MQVKTRVPPVLARPLLLSQRLHCGQRQSRSRATPHAISKAPAAM
                     VTGTDTFTSAWSCRDSCAQYAAIQEAAVIASKTSMVKRVLRAWVGWVRRSIAEGCLVV
                     MR"
     gene            complement(389..1324)
                     /locus_tag="PK818_10480"
     CDS             complement(389..1324)
                     /locus_tag="PK818_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017165054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HQY83193.1"
                     /translation="MCYSAQALQDYRIYVRTHGAALGLDAFIRLFWDWRQNRTGYRLP
                     KAMLDALADPASTPDPRLRQWIQDWTTEQANVLEQELFAQMTRRVGAERALQARVTKK
                     AQQDLRIASDKIARAGQRLKDLRRSEPLPRDSRIFPGSYSCVMVAEGGRRVVYPMRYQ
                     CRPQGKPAAYDRKYPGTYNARRDSLEGYWEDLFGHHHAVMQVDTFYENVEAGDGRNRV
                     LQFTPRDGGPMYVACLWSRWTDPAGTQPDLLSFAAITDEPEPEVAAAGHDRTIINLKP
                     ECIDAWLNPDPRDLPALYALFDDRQHPYYEHREAA"
     gene            complement(1347..2390)
                     /locus_tag="PK818_10485"
     CDS             complement(1347..2390)
                     /locus_tag="PK818_10485"
                     /inference="COORDINATES: protein motif:HMM:NF004785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HQY83194.1"
                     /translation="MHTRRVLLRVLGLALCWLCTLSAIAAAPATPSADGLRPGQFTWT
                     PELAPQGPVAVIVSLPEQRAYVYRNGVRIGVSTTSTGKPGHATPPGIYTILQKRREHY
                     SNLYDDAPMPFMQRLSWDGVALHAGNLPGYPASHGCVRLPLGFAERLFATTGIGSVVV
                     VADAASAPPALVSPGLFAPIDSATGAPRPPAPAPRNGEWLPERAPSGPLTILLSTADR
                     QVVVLRNAVEIGRAALLLDNTAPAIGTRAYLLLHASPGTATTRPGLPAARWQALSLPE
                     TGGIGEDALRELFASQRIALPEGFAGALQAALQPGTTVLLTDQPLQPNAGTGEMLSIE
                     PTANEAAPRAGEP"
BASE COUNT          315 a          843 c          965 g          389 t
ORIGIN      
        1 gccgcatgac caccaggcac ccttccgcga tggaacgccg gacccacccg acccatgcgc
       61 gcagcacgcg cttgaccatg ctggtcttgc ttgcgattac ggcggcctcc tggatggccg
      121 cgtactgcgc gcaggagtcc ctgcaggacc aggcgctggt gaacgtgtcc gtgccggtca
      181 ccatggccgc gggtgccttg ctgatggcgt gcggggtggc gcgggacctg ctttgccgtt
      241 gcccgcaatg caggcgctgg ctcaggagca gggggcgcgc cagcacgggc ggtacccggg
      301 tcttcacctg caccgcctgc gggatcgatt gggacgccaa ggtacaggta agcagtgccg
      361 gcgaaccctg acgaaatccg gtgcgcgctc aggcggcttc gcggtgttcg tagtaggggt
      421 gctggcggtc gtcgaacagg gcgtacaggg ccggcaggtc gcgtgggtcg gggttgagcc
      481 aggcgtcgat gcattcgggc ttgaggttga tgatggtgcg gtcgtggccg gcggcggcga
      541 cttccggttc cggttcgtcg gtgatggcgg cgaacgagag caggtccggc tgcgtgccgg
      601 ccgggtcggt ccagcgtgac cacaggcagg ccacgtacat ggggccgccg tcgcgcgggg
      661 tgaactgcag cacgcggttg cggccgtccc cggcttccac gttttcgtag aaggtgtcca
      721 cctgcatgac cgcgtggtgg tggccgaaca ggtcctccca gtagccctcc aggctgtcgc
      781 ggcgggcgtt gtaggtgccg gggtacttgc ggtcgtacgc ggccggcttg ccttgcgggc
      841 ggcactggta gcgcatcggg tagaccacgc gccggccgcc ttcggcgacc atcacgcagc
      901 tgtagctgcc ggggaagatg cgcgaatcgc gcggcagcgg ctcgctgcgg cgcaggtcct
      961 tcaggcgctg gccggccctc gcgatcttgt cgctggcgat gcgcaggtcc tgctgcgcct
     1021 tcttcgtcac ccgggcctgc agcgcgcgtt cggcgcctac ccgccgggtc atctgcgcga
     1081 acagttcctg ttccagcacg ttggcctgtt cggtcgtcca gtcctggatc cactggcgca
     1141 ggcgcgggtc cggggtgctg gcggggtcgg cgagggcgtc cagcatggcc ttcggcaggc
     1201 ggtagccggt gcggttctgg cgccagtccc agaacagccg gatgaaggcg tccaggccca
     1261 gtgccgcgcc gtgcgtgcgc acgtagatgc ggtagtcctg cagggcttgc gcggaatagc
     1321 acatgggtcg gctcggtggc gccggctcag ggctcgcccg cgcgcggcgc ggcttcattg
     1381 gcggtgggtt cgatgctgag catctcgccg gtgcccgcgt tcggctgcag cggctggtcg
     1441 gtcagcagca ccgtggtgcc cggttgcagg gccgcctgca acgcgccggc gaagccttcg
     1501 ggcagggcga tgcgctggct ggcgaacagc tcgcgcaggg cgtcctcgcc gatgccgccg
     1561 gtttccggca gcgacagcgc ctgccagcgt gccgcgggca gcccgggccg cgttgtggcc
     1621 gtgccggggc tggcgtgcaa cagcaggtag gcgcgggtac cgatcgccgg cgcggtgttg
     1681 tccagcagca gcgccgcgcg cccgatctcc accgcgttgc gcaggaccac cacctggcgg
     1741 tcggcggtgc tgagcaggat cgtcagcggg cctgacggcg cacgttccgg cagccactcg
     1801 ccgttgcgcg gcgccggtgc cggcggccgc ggtgcgccgg tggcgctgtc gatgggggcg
     1861 aacaggcccg gcgacaccag cgcgggcggc gcgctggccg catcggccac caccaccacg
     1921 ctgccgatcc cggtggtggc gaacaggcgc tcggcgaagc ccagcggcag ccgcacgcag
     1981 ccgtgcgagg cggggtagcc cggcaggttg ccggcgtgca gcgccacccc gtcccagctc
     2041 agccgctgca tgaacggcat cggcgcgtcg tcgtacaggt tggagtagtg ctcgcggcgc
     2101 ttctgcagga tggtgtagat gccgggcggg gtggcatgcc cgggcttgcc ggtgctggtg
     2161 gtggacaccc cgatgcgcac gccgttgcgg tacacgtagg cgcgctgctc cggcaggctg
     2221 acgatcaccg cgaccggccc ctgcggcgcg agctccggcg tccaggtgaa ctggccgggg
     2281 cgcaggccgt ccgccgacgg cgttgccggt gcggctgcaa tcgccgacag cgtgcacagc
     2341 cagcacagcg ccaggcccag gacccgcaag aggacgcggc gcgtgtgcat cggacgggtc
     2401 ccgctcagcc cacgtgccgc gccgccagct gccgcagcac gtactgcagc aggccgccgt
     2461 ggcggaagta ctcgacttcc ttcggggtca gcagcagcac gtccgcctgg aa
//
LOCUS       DAORUK010000220         2507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_62005_length_2507_cov_19.475122, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000220 DAORUK010000000
VERSION     DAORUK010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62005_length_2507_cov_19.475122"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PK818_10490"
     CDS             <1..777
                     /locus_tag="PK818_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQY83195.1"
                     /translation="GVRLCKARRYRYANCDMADLEQQLQQARADGARTIMITTDGVFS
                     MDGFIAPLDEITALAKQYGALVHIDECHATGFLGATGRGSAEVKGVLDKIDIFTGTLG
                     KAMGGALGGFTTAKKEVIELLRQRSRPYLFSNSLPPHVVAAGIKAFEMLAAAGELRDR
                     LRDNTAHFRERMTAAGFDIKPGVHPISPVMLYDAPLAQKFAQRLLEEGIYAIGFFFPV
                     VPKGQARIRTQISAAHTREHLDRAIGAFIRIGNELGVLKG"
     gene            complement(781..1512)
                     /locus_tag="PK818_10495"
     CDS             complement(781..1512)
                     /locus_tag="PK818_10495"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQY83196.1"
                     /translation="MAGKGGARYGLARVLSKRGLCSRSEAERLVREGRVRLDGRVVRD
                     PQHPTDAVQAAIQIDGFQAAAARRHYLLLNKPRGLVTTLRDEQGRDTVYRCFDGAGLG
                     FIAPVGRLDKASEGLLLFSNDPQWAARITDPATGPDKTYHVQVDCIPGPARLQAMAEG
                     VLVEGERLHASSAALLRAGEKHAWLEIVLDEGRNRQIRRLLAALGIGVLRLVRVAIGP
                     LALGELPKGQWRELTEQEIAALGDV"
     gene            complement(1531..2037)
                     /locus_tag="PK818_10500"
     CDS             complement(1531..2037)
                     /locus_tag="PK818_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83197.1"
                     /translation="MSTRRLLPVLLLSAMPMVSFAQAATLCSSELMVASPVRPNLLSP
                     VAIELVSARAQLGGASGVLSQAFDESLSVDNVLLRIRQDGCRAVANAKPAGSAVDASD
                     PAAYKPKTEFDNTPWRFNMTQNGKRMTADEFDAWMKARGVRVVGRKDPAAVPATPDPA
                     ATAPKPSN"
     gene            complement(2161..>2507)
                     /locus_tag="PK818_10505"
     CDS             complement(2161..>2507)
                     /locus_tag="PK818_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQY83198.1"
                     /translation="SIDLKGVNFDFDKSTLRPDAVAILNEAVEILKRYPELRVEVAGH
                     TDECGKDEYNQGLSQRRSQVVYDYLTSNGVDAGRLAGPTGYGESRPLEALGDAFPACK
                     SETNRRTELNSQ"
BASE COUNT          402 a          892 c          819 g          394 t
ORIGIN      
        1 ggcgtgcgcc tgtgcaaggc caggcgctac cgctacgcca actgcgacat ggccgacctg
       61 gagcagcagc tgcagcaagc cagggccgat ggcgcgcgca cgatcatgat caccaccgac
      121 ggcgtgttct cgatggacgg cttcatcgcc ccgctggacg agatcaccgc actggcgaag
      181 cagtacggcg cgctggtgca catcgacgaa tgccacgcca ccggcttcct cggtgccacc
      241 ggtcgcggca gcgccgaggt caagggcgtg ctggacaaga tcgacatctt caccggcacc
      301 ctcggcaagg cgatgggtgg cgcgctgggc ggcttcacca cggcgaagaa ggaagtgatc
      361 gaactgctgc gccagcgctc gcggccgtat ctgttctcca actcgctacc gccgcacgtg
      421 gtcgccgccg gcatcaaggc gttcgagatg ctggcagccg ccggcgagct ccgcgaccgg
      481 ctgcgtgaca acaccgccca tttccgcgag cgcatgacgg ccgccggttt cgacatcaag
      541 cccggcgtgc acccgatcag cccggtgatg ctgtacgacg cgccgctggc gcagaagttc
      601 gcccagcgcc tgctggaaga aggcatctat gcaatcggct tcttcttccc ggtggtgccc
      661 aagggccagg cccgcatccg cacccagatt tcggcggcgc atacgcgcga acacctggat
      721 cgcgccatcg gtgcgttcat ccgcatcggc aacgagctgg gcgtgctgaa gggataagcc
      781 tcaaacgtcg cccagcgcgg cgatttcctg ctcggtcagt tcgcgccact ggcccttcgg
      841 cagttcgccc agggccagcg gcccgatcgc cacccgcacc agccgcagca cgccgatgcc
      901 gagtgccgcc agcaggcggc ggatctgccg gttgcggccc tcgtccagca cgatctccag
      961 ccacgcatgt ttttccccgg cgcgcagcag cgcggccgaa cttgcatgca ggcgctcgcc
     1021 ctcgaccagc acgccctccg ccatcgcctg caggcgcgcg gggcctggga tgcagtccac
     1081 ctgcacgtgg taggtcttgt ccggccccgt cgccgggtcg gtgatgcgcg ccgcccactg
     1141 cggatcgttg ctgaacaaca gcagcccctc gctggccttg tcgaggcgcc cgaccggcgc
     1201 gatgaatccc aggccggccc cgtcgaagca acgatagacg gtatcgcggc cctgttcgtc
     1261 gcgcagggtc gtcaccagcc cgcgcggctt gttgagcagc aggtagtgcc gccgggcggc
     1321 ggccgcctgg aagccatcga tctggatcgc ggcctggact gcatcggttg gatgctgtgg
     1381 gtcgcgaacc acgcgtccgt cgagccgcac gcgcccttcg cgcaccagcc gctccgcttc
     1441 gctgcgcgag cacaacccgc gcttggacag cacgcgcgcc agcccgtaac gtgcgccgcc
     1501 cttcccggcc atgcgaccgg aatcgccgcg tcagttggac ggcttggggg cggtcgcggc
     1561 cggatcaggc gtggccggca ccgccgccgg atccttgcgc ccgaccacgc gcacgccgcg
     1621 cgccttcatc cacgcatcga actcatcggc ggtcatgcgc ttgccgttct gggtcatgtt
     1681 gaaccgccac ggcgtgttgt cgaattcggt cttgggcttg taggcggcgg gatcgctcgc
     1741 gtccaccgcg ctgcccgcgg gcttcgcgtt cgccaccgcg cggcagccgt cctggcggat
     1801 tcgcaacaac acgttgtcca ctgacagcga ttcatcgaac gcctgcgaca gcacgccact
     1861 ggcgccaccc aactgggctc ttgccgacac cagctcgatc gccaccggcg acagcaggtt
     1921 gggtcgaacc ggggacgcca ccatcagttc gctgctgcac agggttgccg cctgcgcgaa
     1981 cgacaccatc ggcatggcgg acaggagcag cacgggcagc aggcgacgag tcgacatgag
     2041 gttccccaag ggttggccgc agtgtaggcc ggccggcgca ggcgcggcaa tcgcgacgcg
     2101 caaaaagacg aagcccggca caaggccggg cttcgcggat tgcagaacca acaaccagga
     2161 ttactggctg ttcagctcgg tacggcggtt ggtctcgctc ttgcatgccg ggaaggcatc
     2221 gccgagcgct tccagcggac ggctctcgcc gtagccggtc gggccagcca ggcggccagc
     2281 gtccacgccg ttgctggtca ggtagtcata gaccacctgc gagcgacgct gcgacaggcc
     2341 ctggttgtac tcgtccttgc cgcattcgtc ggtgtggcca gccacttcga cgcgcagttc
     2401 cggatagcgc ttcaggatct cgacggcctc gttcaggatc gccaccgcgt cgggacgcag
     2461 ggtcgacttg tcgaagtcga agttcacgcc cttcaggtcg atcgaga
//
LOCUS       DAORUK010000221         2493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_62523_length_2493_cov_18.564807, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000221 DAORUK010000000
VERSION     DAORUK010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62523_length_2493_cov_18.564807"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..1296)
                     /gene="hemE"
                     /locus_tag="PK818_10510"
     CDS             complement(235..1296)
                     /gene="hemE"
                     /locus_tag="PK818_10510"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HQY83199.1"
                     /translation="MKNDRFLRALRREPVDCTPVWLMRQAGRYLPEYRATRKAAGSFL
                     AMAKNPELACEVTLQPLRRFPLDAAILFSDILTVPDAMGLGLYFVDGEGPKFERPLRT
                     ATQIDALAVPDMETELRYVMDAVRLIRRELDGSVPLIGFSGSPWTLACYMVEGGGSKD
                     WGKVKALALNEPAAMHRLLAVVTDAVIAYLSAQHAAGAQALQVFDTWGGALGPAMYRE
                     FSLPYLTRIARALKDLGAPVILFGKGNAPHLDDLFASGADAIGVDWTVTLEDAARRAN
                     GRVALQGNLDPAILYGNPDAIRSQVQATLDSYATGNGGSREGHVFNLGHGMSPDMDPG
                     HVAVLVEAVHAGSLERSLG"
     gene            complement(1316..1570)
                     /locus_tag="PK818_10515"
     CDS             complement(1316..1570)
                     /locus_tag="PK818_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HQY83200.1"
                     /translation="MRLLLQQRPDGREAPRFVQLQLQPDLLGGWTLLRETGQIGGRST
                     LKREQYADQASALTALEVARDAQLKRGFQLMFAQGADAPR"
     gene            complement(1604..>2493)
                     /gene="aroB"
                     /locus_tag="PK818_10520"
     CDS             complement(1604..>2493)
                     /gene="aroB"
                     /locus_tag="PK818_10520"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HQY83201.1"
                     /translation="IALHVFDAGEASKTLANFSGVIDALAALGATRDACVYALGGGVV
                     GDLAGFAAACWMRGIDCVQLPTTLLAMVDSSVGGKTAVDIAQGKNLVGAFHPPRAVIA
                     DTSTLRTLPPRELRAGFAEVIKYGAIVDAGFLHWLEVNADALLASDDGLLAEAIAHSC
                     VHKAAITERDPFEHGERALLNFGHTFGHAIEAEQGYGGLNHGEAVAVGMLLAARLSAA
                     LRLADAGDGERLRALLQRFGLPTTVPAGLQAEALVARMRLDKKAQGSGLRFVLWDRAG
                     AARVVAGVDEAQVLDVLRG"
BASE COUNT          376 a          898 c          859 g          360 t
ORIGIN      
        1 ggcggtggtg cctccacgcc atacccgacg ttcactcccg cgcagccgcc ggtggcaacg
       61 acggcgacca gcacggcaag ggtggctgga cgcaagatcc cgcggagcca gcggaccacc
      121 atggagcaac ctgacatgcg catccccttg gcaacagccg gcgtcccccg acggcacccg
      181 cagggtagcg cgttcatgct gcaggtgggc gcgactgcga tatgcgggcg tcgcttaccc
      241 gaggctgcgc tcgaggctcc ccgcgtgcac cgcctccacc agcacggcca cgtgcccggg
      301 atccatgtcc ggcgacatgc cgtggccgag gttgaacaca tggccctcgc gcgagccgcc
      361 gttgccggtg gcgtaactgt ccagcgtggc ctgcacctgc gagcggatcg cgtcgggatt
      421 gccgtacagg atcgccgggt ccaggttgcc ctgcagcgca acgcggccat tggcgcggcg
      481 ggcggcgtct tccagggtca ccgtccagtc cacgccgatc gcatccgcgc cgctggcgaa
      541 caggtcgtcc aggtgcggtg cattgccctt gccgaacagg atcaccggcg cgccgaggtc
      601 cttcagcgcg cgcgcgatcc gggtcaggta gggcagcgag aattcgcggt acatcgccgg
      661 gccgagtgcg ccaccccagg tatcgaacac ctgcagcgcc tgcgcgccgg ccgcgtgctg
      721 ggcggacagg taggcgatga ccgcgtcggt caccacggcc agcaacctgt gcatcgccgc
      781 cggctcgttc agcgccagcg ccttgacctt gccccagtcc ttgctgccgc cgccctcgac
      841 catgtagcag gccagcgtcc acggactgcc ggagaaaccg atcagcggca cgctgccgtc
      901 cagttcgcgg cggatcaggc gcaccgcgtc catcacgtag cgcagctcgg tctccatgtc
      961 cggcacggcg agcgcatcga tctgggtcgc cgtgcgcagc gggcgttcga acttcgggcc
     1021 ttcgccgtcc acgaaataca ggcccaggcc catcgcatcc ggcacggtga ggatgtcgga
     1081 gaacaggatc gccgcgtcca gcgggaaccg gcgcagcggc tgcagggtga cttcgcaggc
     1141 cagttccggg ttcttggcca tcgccaggaa gctgccggcc gccttgcggg tggcgcggta
     1201 ctccggcagg tagcggccgg cctggcgcat cagccacacg ggggtgcagt ccacgggttc
     1261 gcggcgcagc gcgcgcagga agcggtcgtt cttcagggtc acaaatgaat tccggttagc
     1321 ggggcgcgtc ggcgccctgg gcaaacatca attggaagcc gcgcttgagc tgtgcatcgc
     1381 gcgccacttc cagcgcggtc agggcgctgg cctggtcggc gtactgctcg cgcttgagcg
     1441 tgctgcgtcc gccgatctgc ccggtttcgc gcagcagcgt ccagccaccc agcaggtcgg
     1501 gctggagctg gagctggacg aatcgcgggg cttcgcggcc gtcggggcgt tgttgcagca
     1561 gcaggcgcat ccgcgaattg taaggcctgt gcctcttgcg ggctcagccg cgcaggacgt
     1621 ccagcacctg cgcttcatcg acgccagcca ccacccgcgc cgcgccggcg cgatcccaca
     1681 gcacgaagcg caggcccgaa ccctgcgcct tcttgtccag ccgcatgcgc gcgaccagcg
     1741 cttcggcctg caggcccgcc ggcaccgtgg tcggcaggcc gaaccgctgc agcagcgcgc
     1801 gcaggcgttc gccatcgccg gcatccgcca ggcgcagtgc ggccgacagc cgggcggcca
     1861 gcagcatccc caccgcgact gcctcgccgt ggttcagccc gccatagcct tgctcggcct
     1921 cgatcgcgtg gccgaaggtg tggccgaagt tgagcagcgc gcgctcgccg tgttcgaacg
     1981 ggtcgcgttc ggtgatcgcg gccttgtgca cgcagctatg ggcgatggcc tccgccagca
     2041 acccgtcgtc gctggcgagc agggcatcgg cattcacctc cagccagtgg aggaagccgg
     2101 catcgacgat cgccccgtac ttgatcacct cggcgaagcc cgcgcgcagt tcgcgtggcg
     2161 gcagcgtgcg cagggtcgag gtgtcggcga tcaccgcgcg cggcggatgg aacgcaccga
     2221 ccaggttctt gccctgcgcg atgtccaccg cggtcttgcc gccgaccgag gaatccacca
     2281 tcgccagcag ggtggtcggc agctgcacgc agtcgatccc gcgcatccag caggccgcgg
     2341 cgaagccggc caggtcaccg accacgccac cgccgagcgc gtacacgcag gcgtcgcggg
     2401 tggcgccgag cgcggccagc gcgtcgatca cgccggaaaa attcgccagc gtcttggagg
     2461 cttcgccggc atcgaacacg tgcagcgcga tcc
//
LOCUS       DAORUK010000222         2489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_62716_length_2489_cov_17.624486, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000222 DAORUK010000000
VERSION     DAORUK010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62716_length_2489_cov_17.624486"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="PK818_10525"
     CDS             complement(<1..122)
                     /locus_tag="PK818_10525"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HQY83202.1"
                     /translation="MNRSLTAIAALLLATALPSDAQQRVYQWKDAKGVTHYTDMP"
     gene            196..990
                     /gene="pssA"
                     /locus_tag="PK818_10530"
     CDS             196..990
                     /gene="pssA"
                     /locus_tag="PK818_10530"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003491009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HQY83203.1"
                     /translation="MDEHPDEHHDHLPARPRSRGIYLLPNLFTTGGLFAGFFAIIAAS
                     QGRIEAACVAIFVAGVLDGVDGRVARLTNTQTEFGVQYDSLADLVSFGMAPALVMYHW
                     ALVSMKLDGATMGKLGWLAAFLYAACAALRLARFNSQVGQVDKRWFIGLASPAAAGLM
                     ASFVWTMHDLGLQGPELRHAALAVTITAGLLMVSRIRYSSFKGSGSGPKADRVPFFAL
                     LIVVVVLIALTIDPPKTLLATAMLYALSGPLLWFRRRKLPPQEPVA"
     gene            987..1259
                     /locus_tag="PK818_10535"
     CDS             987..1259
                     /locus_tag="PK818_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83204.1"
                     /translation="MSWDGFQREVLAELGHVLYVPLHARQAGAQAAMGAIDEAMLARI
                     ARAAGVDAEGLRAHADIAGASAGLRGNASAKRALWPRLRALRRHPG"
     gene            1265..1738
                     /gene="rimI"
                     /locus_tag="PK818_10540"
     CDS             1265..1738
                     /gene="rimI"
                     /locus_tag="PK818_10540"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HQY83205.1"
                     /translation="MCAIDAPCDASLRPMREADLDAVMRIEARAYPFPWTPGIFRDCL
                     RAGYPMWVQENAGAIVGYGVLSIAADEAHLLNLCTAPGHEGRGLARRMLQALLRVARG
                     GGAQRVFLEVRPSNPRAIELYDRSGFNEIGRRPRYYPAANNGREDAIVMAMELLD"
     gene            complement(1735..>2489)
                     /locus_tag="PK818_10545"
     CDS             complement(1735..>2489)
                     /locus_tag="PK818_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012479102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQY83206.1"
                     /translation="FDLLAQCLYEFTWNQVCDWFLELAKPALNGDDAGAAASTRHTLA
                     HVLEALLRMLHPLVPFVTEELWQAVAPTLGKSGSIMLQAYPRAGEVEAAAYAQAEADI
                     EWLKAMVSAIRRIRSELGVSPARQVSLLLRGGGEAECARLGRLEAQLRFLCKLERIDT
                     LAGEPPAAAPAVVGELQLFVPLEGLVDLDAERVRLDKDIARVAAEKDKSEAKLARFGA
                     GVPAAVIEQERARLADWSAKLDALTTQRTRLD"
BASE COUNT          338 a          872 c          892 g          387 t
ORIGIN      
        1 ggcatgtcgg tgtagtgggt cacgcccttg gcgtccttcc actggtagac gcgctgctgt
       61 gcgtccgagg gcagtgcggt cgccagcagc agggcggcga tggcggtcag ggaacggttc
      121 atggcgcact ccgggggtag ccggatccga ttgcagcacc gcgccggtgc cgctgcaagc
      181 ccttacactg gccgcatgga cgaacacccc gacgagcacc acgaccacct gccggcacgt
      241 ccgcgcagcc gcggcatcta cctgctgccc aacctgttca ccaccggcgg cctgttcgcc
      301 ggcttcttcg ccatcatcgc cgcctcgcag gggcgcatcg aggcggcgtg cgtggcgatc
      361 ttcgtggccg gggtgctgga cggggtggat ggccgcgtcg cccgcctgac caatacccag
      421 accgagttcg gcgtgcagta cgactcgctg gcggacctgg tcagcttcgg gatggcgccg
      481 gcgctggtga tgtaccactg ggcgctggtg tcgatgaagc tggacggcgc gaccatgggc
      541 aagctgggct ggctggctgc attcctgtac gccgcctgtg cggcgctgcg gctggcgcgc
      601 ttcaacagcc aggtcgggca ggtcgacaag cgctggttca tcggcctggc cagccccgcc
      661 gctgcaggct tgatggcgag ttttgtgtgg acgatgcacg acctcggcct gcaaggtccc
      721 gagctgcgcc atgccgcgct ggcggtgacg atcaccgccg gcctgctgat ggtgagccgg
      781 atccgctaca gcagcttcaa gggcagtggc agcgggccga aggccgaccg ggtgccgttc
      841 ttcgccctgc tgatcgtggt ggtggtcctg atcgcgctca ccatcgatcc tcccaagacc
      901 ctgctcgcga ccgccatgct gtatgcgctg tccggcccgc tgctgtggtt ccgccgtcgc
      961 aagctgccgc cgcaggaacc ggttgcgtga gctgggacgg gttccagcgc gaggtgctgg
     1021 ccgagctcgg ccatgtgctc tacgtcccgc tgcacgcacg gcaagcgggg gcgcaggctg
     1081 ccatgggtgc catcgacgag gccatgctgg cgcggatcgc gcgtgcggca ggcgtggatg
     1141 cggagggcct gcgcgcgcat gcggacatcg ccggggcaag cgcgggcttg cgtggcaacg
     1201 ccagcgccaa gcgcgcgctg tggccgcgcc tgcgcgcact gcgacggcac ccggggtgag
     1261 cgcggtgtgc gcgatcgatg cgccgtgcga cgccagcctg cggccgatgc gcgaggcgga
     1321 cctggacgcg gtgatgcgga tcgaggcgcg cgcctacccg ttcccgtgga cgcccgggat
     1381 cttccgtgac tgcctgcgcg ccggctatcc gatgtgggtg caggaaaacg cgggggcgat
     1441 cgtgggctac ggggtgctca gcatcgccgc cgacgaagcg cacctgctca acctgtgcac
     1501 cgccccggga cacgaggggc gcggactggc gcggcgcatg ttgcaggcgc tgctgcgggt
     1561 cgcgcgtggt ggcggcgcgc agcgcgtgtt cctcgaggtg cggccgtcga acccgcgcgc
     1621 gatcgagctc tacgaccgca gcggcttcaa cgagatcggc cgccgcccgc gctactaccc
     1681 cgccgccaac aacggccgcg aggacgccat cgtgatggcg atggaattgc tggactagtc
     1741 cagccgcgtg cgctgggtgg tcagcgcgtc cagcttggcg gaccagtcgg ccaggcgcgc
     1801 gcgctcctgc tcgatcaccg ccgcgggaac gccggcgccg aacctggcca acttcgcctc
     1861 gctcttgtcc ttctcggccg ccaccctggc gatgtccttg tccagccgca cgcgctcggc
     1921 atcgaggtcg accaggcctt ccagcggcac gaacagctgc agctcgccga ccaccgcggg
     1981 cgcggcggcc ggcggctcgc cggcgagcgt gtcgatgcgt tccagcttgc acaggaagcg
     2041 cagctgcgcc tccagcctgc ccagccgcgc gcattcggcc tcaccaccgc cccgcagcag
     2101 cagcgacacc tgcctggccg gcgacacccc gagctcgctg cggatgcggc ggatcgcgct
     2161 gaccatcgcc ttcagccatt cgatgtcggc ctcggcctgc gcgtaggcgg cggcttcgac
     2221 ctcgcccgcc cgtggatacg cctgcagcat gatgctgccg gacttgccga gcgtcggcgc
     2281 gaccgcctgc cacaattcct cggtcacgaa cgggaccagc gggtgcagca tccgcagcag
     2341 cgcttcgagc acgtgggcca gggtgtggcg ggtgctcgcg gcggcacccg cgtcgtcgcc
     2401 attcagggcc ggcttggcca gctccaggaa ccagtcgcaa acctggttcc aggtgaattc
     2461 gtacaggcat tgcgccagca ggtcgaagc
//
LOCUS       DAORUK010000223         2488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_62760_length_2488_cov_19.092478, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000223 DAORUK010000000
VERSION     DAORUK010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62760_length_2488_cov_19.092478"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..802)
                     /gene="queC"
                     /locus_tag="PK818_10550"
     CDS             complement(92..802)
                     /gene="queC"
                     /locus_tag="PK818_10550"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HQY83207.1"
                     /translation="MTASRKPAVALVSGGMDSAVVLAIAREAGFDVHALSVRYGQRHT
                     SELDAADRVSRALGAVAHKTVQVDLRSLGGSALTDDGIQVPTDDDGHVIGQGIPSTYV
                     PARNTIMLSVALGWAEVLGSCDLFCGVNAVDYSGYPDCRPEFIAGFEALANLATRAGV
                     EGSRFRVHAPLMRMSKADIVREGLRLGVDFAQTVSCYQADADGRACTHCDACRLRAQG
                     FAEAGVADPTRYVDGAIG"
     gene            complement(799..1473)
                     /gene="queE"
                     /locus_tag="PK818_10555"
     CDS             complement(799..1473)
                     /gene="queE"
                     /locus_tag="PK818_10555"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HQY83208.1"
                     /translation="MNAVADAAAASAERLKLTEIFLSLQGEARDAGWPTVFVRLTGCP
                     LRCQYCDTAYAFHGGQWWTLDAILAEVARHGVRHVCVTGGEPLAQKRCLPLLSRLCDA
                     GYSVSLETSGAIDIGGVDPRVSRVLDIKTPGSAEVERNLWANLALLTPRDQVKFVLCS
                     RADFDWAKGVLSEHRLHDVCEVLFSPSATQLPARELADWIVADKLPVRFQVQLHKLLW
                     NDEPGR"
     gene            complement(1483..2256)
                     /gene="ybgF"
                     /locus_tag="PK818_10560"
     CDS             complement(1483..2256)
                     /gene="ybgF"
                     /locus_tag="PK818_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HQY83209.1"
                     /translation="MRMRSSLVLLAAVLLASPLAQAQRASLADRVSALEARNAGDQGG
                     TELVNQLSQLRAEVQELRGQIELLQQQLEQAKQGQRSQYLDLDGRINRIEAGTAPGTG
                     APAPSAIPAAAADAPAAPPGSSAMAGKDERSAYAHAFDALKAGDYAESARRFRDFLAV
                     HPGGQLAPNALYWLGESYYVTQNYALAGEQFQALLDRYPRHDKAAGALLKLGLAQYGL
                     KDDAGAEATLRQVVQRYPGSDVARTADDRLRAMQISGAR"
     gene            complement(2256..>2488)
                     /locus_tag="PK818_10565"
     CDS             complement(2256..>2488)
                     /locus_tag="PK818_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQY83210.1"
                     /translation="ARLRLEGNADERGSREYNLGLGERRGNAVSSALQANGGAGSQLS
                     VTSYGEERPVCNDSGEGCWSQNRRVELVYSAK"
BASE COUNT          371 a          885 c          862 g          370 t
ORIGIN      
        1 aaccatcgac ctacggatta aaagtccgca gctctaccac tgagctaacg gcccgtgatg
       61 cgcatcccgg cggtttgccg gggcgcgaat tctaaccgat ggcgccgtcg acgtagcgcg
      121 tggggtcggc caccccggcc tcggcgaacc cctgcgcacg caggcggcag gcatcgcagt
      181 gggtgcaggc gcggccgtcg gcgtcggcct ggtagcaact gacggtctgc gcgaagtcca
      241 cccccaggcg caggccctcg cggacgatgt cggccttgct catgcgcatc agtggcgcat
      301 gcacgcggaa gcggctgcct tcgacgcccg cgcgggtggc caggttcgcg agcgcctcga
      361 agccggcgat gaactccggc cggcaatccg ggtagccaga gtagtccacc gcgttgaccc
      421 cgcagaacag gtcgcagctg cccagcacct cggcccagcc cagcgccacc gacagcatga
      481 tggtgttgcg cgcgggcacg taggtcgacg ggatgccctg cccgatcacg tggccatcat
      541 cgtcggtcgg gacctggatg ccatcgtcgg tcaacgcgga accgccgagg ctgcgcaggt
      601 ccacctgcac cgtcttgtgc gcgaccgcgc ccaacgcccg ggacacgcga tccgccgcat
      661 ccagttccga ggtatgccgc tggccgtagc gcacgctcag cgcgtgcacg tcgaagccgg
      721 cctcgcgggc gatggccagc accacggccg agtccatgcc gccggagacc agggccacgg
      781 cgggtttgcg ggatgcggtc atcggccggg ctcgtcgttc cacaggagtt tgtgcagttg
      841 cacctggaag cggaccggca gcttgtccgc cacgatccag tccgccagct cgcgcgccgg
      901 cagctgcgtc gcgctgggcg agaacagcac ctcgcagaca tcgtgcaggc ggtgttcgct
      961 gaggacgccc ttcgcccagt cgaaatccgc gcgcgagcag agcacgaact tcacctggtc
     1021 gcgcggggtc agcagcgcga ggttggccca caggttgcgt tcgacctcgg ccgaacccgg
     1081 ggtcttgatg tcgagcaccc gcgacacccg cggatccacc ccgccgatgt cgatcgcccc
     1141 cgaggtttcc agcgacacgc tgtagccggc atcgcacaac ctggaaagca gcggcaggca
     1201 gcgcttctgc gccagcggct cgccgccggt cacgcagaca tggcgcacgc cgtggcgggc
     1261 gacctcggcg aggatcgcgt caagcgtcca ccactggccg ccgtggaaag cgtaggcggt
     1321 gtcgcagtac tggcaacgca acggacagcc ggtcagccgc acgaacacgg tcggccagcc
     1381 ggcatcgcgg gcttcgccct gcagggacag gaagatctcg gtgagtttca ggcgctccgc
     1441 ggatgcggcg gccgcatcgg cgacagcgtt catgcggata gtctagcggg cgccgctgat
     1501 ctgcatggcc cgcaggcgat cgtccgcggt gcgcgcgacg tcgctgccgg gataacgctg
     1561 caccacctgg cgcaaggtcg cctccgcccc ggcgtcgtcc ttcagcccgt actgggccag
     1621 tcccagcttg agcagtgcgc cggccgcctt gtcgtggcgc gggtagcggt ccagcagcgc
     1681 ctggaactgc tcgccggcca acgcgtagtt ctgggtgacg tagtagcttt cgcccagcca
     1741 gtacagcgcg ttcggcgcga gttggccgcc gggatggacg gccaggaaat cgcggaagcg
     1801 gcgcgcggat tccgcgtagt cgccggcctt gagcgcatcg aaggcatgcg cgtaggcgct
     1861 gcgttcgtcc ttcccggcca tggcgctcga cccaggcggc gctgcaggcg catccgcggc
     1921 cgccgccgga atggccgaag gcgcgggcgc gccagtcccg ggcgcggtgc ctgcctcgat
     1981 ccggttgata cgcccgtcga gatcgaggta ctggctgcgc tggccctgct tcgcctgctc
     2041 cagttgctgc tgcagcagtt cgatctggcc gcgcagctcc tgcacctccg cacgcagttg
     2101 cgagagctgg ttgaccagtt cggtgccgcc ctggtcgccc gcattgcgcg cctccagcgc
     2161 ggacacgcgg tcggccaggc tggcgcgctg ggcctgcgcc agcggcgatg caagcagcac
     2221 cgccgccagc aggacgagcg acgaacgcat ccgcatcact tggcgctgta gaccagttca
     2281 acccggcggt tctgcgacca gcagccttcg ccggagtcgt tgcacaccgg gcgctcctcg
     2341 ccatagctgg tcacgctcag ctggctgccg gcgccgccgt tggcctgcag cgcggaagac
     2401 accgcgttgc cgcggcgctc gccgaggccg aggttgtatt cgcggctgcc gcgctcgtcg
     2461 gcattgcctt ccaggcgcag gcgcgcgg
//
LOCUS       DAORUK010000224         2488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_62762_length_2488_cov_15.477189, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000224 DAORUK010000000
VERSION     DAORUK010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62762_length_2488_cov_15.477189"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="PK818_10570"
     CDS             complement(<1..950)
                     /locus_tag="PK818_10570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQY83211.1"
                     /translation="MTPRRDSYTLLRDTLRWRVALVLGVLVMALMVVLAAVNTSLGLE
                     DTALLAWGIFVATAAGVALLLALPHALGGTIFFCLIVAVLVAVPAFGLYHGRNMQHWA
                     YILPPLLVFLVRATPALLGMVLFGIYVTLTIVPLLPGIDVIRFAAGYGLLVCFMYTYA
                     LLQERAAAMLRYHSDHDALTNCLNRRTFNEALDRLEAARPAAPRCTFLLIDIDHFKSI
                     NDQHGHLVGDRTITQVAAVLGRELDPGTPLFRYGGEEFAVMLEGADGHAGVQLAERLR
                     AAVARADFQGVPVTVSIGVAEWHAAHGSVDAAVGHADQAL"
     gene            complement(1122..2222)
                     /locus_tag="PK818_10575"
     CDS             complement(1122..2222)
                     /locus_tag="PK818_10575"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HQY83212.1"
                     /translation="MSERLLVSTRKGLFVLDRPASGWRIAHSSFLGDNVTLALADPRD
                     GSWYAALNLGHFGVKLRRSTDEGRNWTELAVPAYSAEQTVSTGDGKPPTPATLQLLWA
                     LEAGGAKEPGRLWAGTIPGGLFRSDDHGGSWHLVESLWDMPERANWMGGGYDQPGIHS
                     ICVDPRDPRVLRVAVSTGGVWRSDDGGASWQVAAKGMYATFMPPERREDPTIQDVHRM
                     VQCAGAPDHLWAQHHNAVFHSVDGGQLWHDVPNVPPSVFGFAVAVHPRDPGTAWFVPA
                     VKDECRVPVDGRLVVARTRDGARSFETLREGLPQEGAYDLVYRHGLAIDDSGECLAIG
                     STTGGLWLSEDQGDRWIAPQCRLPPIHALAFA"
     gene            complement(2225..>2488)
                     /locus_tag="PK818_10580"
     CDS             complement(2225..>2488)
                     /locus_tag="PK818_10580"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HQY83213.1"
                     /translation="ARWLQPAGAGPSRELALDVDGATLVAALESLFTRHPVLRGYVLD
                     EHGNVRHHVAVFVDGQSIADKARLDLPLAAQSEVYVMQALSGG"
BASE COUNT          389 a          858 c          902 g          339 t
ORIGIN      
        1 tagagcgcct ggtcggcgtg gcccacggcg gcgtcgacgc tgccgtgggc tgcatgccat
       61 tcggccacgc cgatgctcac ggtcacgggc acgccctgga agtcggcgcg cgccaccgcg
      121 gcgcgcaggc gctcggccag ctgcacgccg gcgtggccgt cggcgccttc gagcatcacc
      181 gcgaactcct cgccgccgta gcggaacagg ggcgtgcccg gatcgagttc gcggccgagg
      241 accgcggcga cctgggtgat ggtgcggtcg ccgacgaggt gaccgtgctg gtcgttgatc
      301 gacttgaagt ggtcgatgtc gatcagcagg aaagtgcagc gcggcgcagc gggtcttgcg
      361 gcctcgagcc ggtccagcgc ctcgttgaag gtacggcggt tgaggcagtt ggtcagcgcg
      421 tcgtggtcgg agtggtagcg cagcatggcc gcggcgcgtt cctgcagcag cgcgtaggtg
      481 tacatgaagc acaccagcag gccatagccg gcggcgaagc ggatgacgtc gatgcccggc
      541 agcagcggca cgatggtcag ggtcacgtag atgccgaaca ggaccatgcc cagcagcgcc
      601 ggcgtggcgc gcaccaggaa caccagcagc ggcggcagga tgtaggccca gtgctgcatg
      661 ttgcggccgt ggtagaggcc gaaggccggc accgcgacca gcaccgcgac gatcaagcag
      721 aagaagatcg tgccgcccag cgcgtgcggc agggccagca gcagcgccac gccggccgcg
      781 gtggcgacga agatgcccca ggccagcagc gcggtgtctt cgagtcccag cgaggtgttg
      841 accgcggcca gcaccaccat cagcgccatc accagcacgc ccagcaccag cgccacgcgc
      901 cagcgcaggg tgtcgcgcag cagggtgtag gagtcgcggc gcggggtcat cggtgaaagg
      961 gcggtacgcg gggcgcccat gctatctcag ggtgcctttg taggagcgca tctcatgcgc
     1021 gaccgcggga cctgggccac ggaatcgctt gaatggctcc cggtgcggat cgggagtccg
     1081 cggtcgcgcg taagacgcgc tcctacaggg aaacggcatc gctacgcgaa ggcgagggcg
     1141 tggatcggcg gcaggcggca ctgcggcgca atccagcggt caccctggtc ctcgctcagc
     1201 cacaagccgc cggtggtgga gccgatcgcg aggcattcgc cactgtcgtc gatcgccagg
     1261 ccatgccggt agacgaggtc gtaggcgccc tcctgcggca gcccctcgcg caaggtctcg
     1321 aagctgcgcg cgccatcgcg cgtgcgcgcc accaccaggc gcccgtcgac cggcacccgg
     1381 cattcgtcct tcaccgccgg cacgaaccac gcggtccccg gatcgcgcgg atgcaccgcc
     1441 accgcgaagc cgaacaccga gggcgggacg ttcggcacgt cgtgccagag ctggccgccg
     1501 tcgacgctgt ggaatacggc gttgtggtgc tgcgcccaca ggtggtcggg cgcgccggcg
     1561 cactgcacca tgcgatgcac gtcctggatg gtcgggtcct cgcgccgctc gggcggcatg
     1621 aacgtcgcat acatgccctt cgccgcgacc tgccagctcg cgccgccgtc gtcgctccgc
     1681 cacacgccgc cggtggacac cgccacgcgc agcacgcgcg gatcgcgcgg gtcgacgcag
     1741 atcgagtgga tgccgggctg gtcgtagccg ccgcccatcc agttggcgcg ctccggcatg
     1801 tcccacagcg actcgaccag atgccaactg ccgccatggt cgtcggagcg gaacagtccg
     1861 cccgggatcg tgcccgccca caggcgcccg ggttccttcg cgccgccggc ttcaagcgcc
     1921 cacagcaact gcaaggtggc cggcgtgggc ggcttgccat cgcccgtgct cacggtctgt
     1981 tcggccgaat acgcggggac ggccagctcg gtccagttgc ggccttcgtc ggtggagcgc
     2041 cgcagcttga ccccgaagtg gccgaggttg agcgccgcgt accagctgcc gtcgcgcggg
     2101 tcggccaggg cgagcgtgac gttgtcgccg aggaaactgc tgtgcgcgat gcgccagccg
     2161 gatgccgggc ggtcgaggac gaagaggccc ttgcgggtcg agaccaggag tcgctcggac
     2221 atgcttatcc tccggacagg gcctgcatga cgtagacctc ggattgcgct gccaacggca
     2281 ggtcgaggcg agccttgtcg gcgatcgact ggccatcgac gaacaccgcc acgtggtggc
     2341 gcacgttgcc gtgctcgtcg agcacgtagc cgcgcagcac cggatgacgc gtgaacaggg
     2401 actccagcgc ggccaccagt gtggccccat ccacgtccag cgccagttcg cgcgatggcc
     2461 cggcgcccgc tggctgcagc cagcgcgc
//
LOCUS       DAORUK010000225         2477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_63232_length_2477_cov_16.650702, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000225 DAORUK010000000
VERSION     DAORUK010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63232_length_2477_cov_16.650702"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /gene="ruvB"
                     /locus_tag="PK818_10585"
     CDS             <1..1026
                     /gene="ruvB"
                     /locus_tag="PK818_10585"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006452374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HQY83214.1"
                     /translation="IAPAATREDEALEASIRPRRLDEYLGQAPVREQMQIYIEAAKGR
                     GEALDHVLIFGPPGLGKTTLSHVIANELGVSLRVTSGPVIEKAGDLAALLTNLQPHDV
                     LFIDEIHRLSPVVEEILYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGATTRAGLL
                     TAPLRDRFGIVQRLEFYSPEELTRIVRRSARILGIACDAEGAGEIARRARGTPRIANR
                     LLRRLRDYAQVKGDGSIDAAIASAAMDMLKVDPEGFDELDRRMLGIIIEAFDGGPVGV
                     ESLAAALSEERGTLEDVIEPYLIQQGFLVRTARGRMATPKAYRHLGFKPKAGSNAGLF
                     EAEKGAE"
     gene            1142..1558
                     /gene="ybgC"
                     /locus_tag="PK818_10590"
     CDS             1142..1558
                     /gene="ybgC"
                     /locus_tag="PK818_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HQY83215.1"
                     /translation="MTTPFRWPVRVYWEDTDAGGVVYHARYLAFMERARSEWMRACGW
                     GQDAMRGREDLVFAVRAMELDFRAPARLDDQLQVSVALVECRGASFVVSQQVLRGGEL
                     LAAARVRIAALAASTFRPRPIPQPLQSELKSLLESP"
     gene            1558..2346
                     /gene="tolQ"
                     /locus_tag="PK818_10595"
     CDS             1558..2346
                     /gene="tolQ"
                     /locus_tag="PK818_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005913648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HQY83216.1"
                     /translation="MPVSTMLQSLPQALPENGDATAVELAGAGAPAAAQVADGGLDML
                     QLILHASLPVQLVMLLLLLASVASWVIILRKKRVLDRAEKEADRFEETFWSGAELPKL
                     YASASERNREIEGLEAIFEGGMREFNRIRQRRGVDARMQLEGAQRGMRAAASRELDGL
                     ERNLEFLANVGSISPYVGLFGTVWGIMISFQGLATMKEATIATVAPGISEALVATAMG
                     LFAAIPAVWAYNRFATRAERMAVRYDTFSEEFSSILQRQAHVED"
     gene            2360..>2477
                     /locus_tag="PK818_10600"
     CDS             2360..>2477
                     /locus_tag="PK818_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQY83217.1"
                     /translation="MTSASRRHRRKKLKAEINVVPYIDVMLVLLIIFMVTAPL"
BASE COUNT          392 a          845 c          860 g          380 t
ORIGIN      
        1 atcgctcccg ccgccacccg cgaagacgag gcgctcgaag ccagcatccg cccgcgccgg
       61 ctggacgaat accttggcca ggcgcccgtg cgcgagcaga tgcagatcta catcgaggcg
      121 gccaagggcc gtggcgaggc gctcgaccac gtgctgatct tcggcccgcc cgggctcggc
      181 aagaccaccc tcagccatgt catcgccaac gagcttgggg tgagcctgcg cgtcacctcg
      241 gggcccgtga tcgagaaggc cggcgacctc gccgcgctgc tgaccaacct gcagccgcac
      301 gacgtgctgt tcatcgacga gatccaccgg ctgtcgccgg tggtcgagga aatcctgtac
      361 ccggcgatgg aggactacca gctcgacatc atgatcggcg agggtccggc cgcgcgctcg
      421 atcaagctcg acctgccgcc gttcaccctg gtcggcgcca ccacccgcgc cggcctgctg
      481 accgcgccgc tgcgcgaccg cttcggcatc gtccagcgcc tggagttcta ttcgccggag
      541 gagctcaccc gcatcgtccg ccgctccgcc cgcatcctcg gcatcgcctg cgacgccgag
      601 ggcgccggcg agatcgccag gcgcgcccgc ggtaccccgc gcatcgccaa ccggctgctg
      661 cggcggctgc gcgactacgc ccaggtcaag ggcgacggca gcatcgacgc cgccatcgcc
      721 agcgcggcga tggacatgct caaggtcgac ccggagggtt tcgacgaact cgaccggcgc
      781 atgctcggga tcatcatcga ggccttcgat ggcggcccgg tcggggtgga gtcgctggcg
      841 gcggcgctgt cggaagaacg cggcaccctg gaagacgtga tcgagcccta cctgatccag
      901 caggggttcc tggtgcgcac cgcgcgcggc cgcatggcca cgcccaaggc ctaccggcac
      961 ctgggcttca agccgaaggc gggaagcaac gcgggcttgt tcgaagcgga aaagggggca
     1021 gagtgaattt gctgccgcga aaaaggggcg agggcaaggt ttcgcgcgaa ggtcatcgcg
     1081 cagtcgaaag aaatcgggcg aaatttactc tggccccttt ttcttgttcg ccttttccgg
     1141 catgaccacg cccttccgct ggcccgtgcg cgtctactgg gaagacaccg acgccggtgg
     1201 cgtggtgtac cacgcgcgct acctggcctt catggagcgc gcgcgcagtg agtggatgcg
     1261 cgcctgcggc tggggccagg acgcgatgcg cgggcgcgag gacctggtgt tcgcggtgcg
     1321 cgcgatggag ctcgacttcc gcgccccggc ccggctcgac gatcagctgc aggtcagcgt
     1381 ggctctggtt gaatgccgcg gggccagctt cgtcgtttcc cagcaggtgc tgcgcggagg
     1441 cgagctgctg gccgcggcga gggtgcggat cgcggcgctg gcggcgtcca ctttccgccc
     1501 gcggccgatc ccgcaaccgc tgcaatccga actgaagtcc ctgctggaga gcccttgatg
     1561 cccgtgtcga ccatgttgca atccctgccg caggcgctgc ccgagaacgg tgatgcaacc
     1621 gcggtggagc tggccggcgc cggcgcgccg gcggctgccc aggtggccga tggcgggctc
     1681 gacatgctgc agctgatcct gcacgccagc ctgccggtgc aactggtgat gctgctgctg
     1741 ttgctggcct cggtcgcctc gtgggtgatc atcctgcgca agaagcgggt gctcgaccgc
     1801 gccgagaagg aagcggaccg cttcgaggaa acgttctggt ccggggcgga attgccgaag
     1861 ctgtacgcca gcgccagcga acgcaaccgc gagatcgaag gactggaggc gatcttcgag
     1921 ggcggcatgc gcgagttcaa ccgcatccgc cagcgccgcg gcgtcgacgc ccggatgcag
     1981 ctggaaggcg cccagcgcgg catgcgcgcg gctgcctcgc gcgaactcga cgggctggaa
     2041 cgcaacctgg agttcctggc caacgtcggt tcgatcagcc cctacgtggg cctgttcggc
     2101 accgtgtggg ggatcatgat ttcgttccag ggcctggcga cgatgaagga ggcgaccatc
     2161 gccaccgtgg ccccgggcat ctccgaggcc ctggtcgcca ccgcgatggg cctgttcgcg
     2221 gcgatcccgg cggtctgggc ctacaaccgc ttcgccaccc gcgccgagcg gatggcggtg
     2281 cgctacgaca ccttcagcga ggagttctcc tcgatcctgc agcgccaggc ccacgtggaa
     2341 gactgaggcg cgcatggcac tgacaagcgc aagccggcgg catcgcagga agaagctgaa
     2401 ggccgagatc aacgtcgtgc cgtacatcga cgtgatgctg gtgctgctga tcatcttcat
     2461 ggtcaccgcg ccgctgc
//
LOCUS       DAORUK010000226         2462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_63852_length_2462_cov_17.794765, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000226 DAORUK010000000
VERSION     DAORUK010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63852_length_2462_cov_17.794765"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..902
                     /locus_tag="PK818_10605"
     CDS             165..902
                     /locus_tag="PK818_10605"
                     /inference="COORDINATES: protein motif:HMM:NF021527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HQY83218.1"
                     /translation="MVASFPAPQTPLDGLDAAMRQRGYAVLAADAVAELAGVAVSEFA
                     ALLPSWDDLRPDAYLKDGGRYRKRRHSCFIAEGDELRQVPHRMHWQSLDYNALHGGMQ
                     RWFEPMATTVVAQPVWSKLLLALAHTCTARMPSPRWCIEAHQFRIDTVDGLGRPTPEG
                     AHRDGVDFVAVLLVARAGIKGGETRVFEAAGPDGQRFTLGEPWSMLLLDDARVIHEST
                     PIQPLGDYGYRDTLVLTFRAAGFQGEV"
     gene            947..1936
                     /locus_tag="PK818_10610"
     CDS             947..1936
                     /locus_tag="PK818_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014091103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQY83219.1"
                     /translation="MKLGSLKEGGRDGTLIVVSRDLTRAVRATGIAPTLQRALEDWES
                     AAPRLNALSDALNDGSADGVFDLDLQALAAPLPRAYEFVDGSAYLPHVARVRRARGAE
                     VPESFYVDPLMYQAVSAGFYGPRDAVKVVSEDYGIDLEAEIVIVTDDVPMAVTSEAAA
                     GHIQLVGLVNDVSLRNLIPGELAKGFGFLQSKPRSALSPVFVTPDELGSHWQGNKLHL
                     PLLTHVNGRWFGAPEAGVDMQFDFAQLVAHAAKTRPLSAGTIVGSGTVANEDTALGAS
                     CFAEQRTVEALRDGKPSTPFMAFGDTVRIEVLDRDGTSIFGAIEQRIERQPQP"
     gene            2038..>2462
                     /gene="maiA"
                     /locus_tag="PK818_10615"
     CDS             2038..>2462
                     /gene="maiA"
                     /locus_tag="PK818_10615"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009594191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HQY83220.1"
                     /translation="MNEALRLYSYWRSSAAYRVRIGLNLKGLAYDIVPVHLLRDGGQQ
                     HSPEYRGINPQQLVPVLGHGHRRLGQSLAILEYLDEMWPAPPLLPSTARERQRVRALS
                     MLVACDIHPLNNLRVLQYLEQEWNVPQPERDEWVRHWIV"
BASE COUNT          368 a          823 c          879 g          392 t
ORIGIN      
        1 ggcacctgac tctgactcag gcattcggtg gttcgaatcc atccggggct gccatctcca
       61 accagacccg ccgcaaggcg ggtttggcgt tttcgggatg cgcatttgcc atgtccggcg
      121 tggtgacgcg ggtgcgacaa tcgcgcttcg caatggagcc gccgatggtt gccagttttc
      181 ccgcgccgca gacgccactc gacggtctcg atgccgccat gcgccagcgc ggctatgcgg
      241 tgctcgcagc ggatgcggtg gccgagctcg ccggcgtggc agtgtccgag ttcgccgcgc
      301 tgctgccgag ttgggacgat ctgcgccccg atgcgtacct gaaggatggc gggcgatacc
      361 gcaagcgccg gcattcctgc ttcatcgccg agggcgatga gctgcgccag gtgccgcatc
      421 gcatgcactg gcaatcgctc gactacaacg ccctgcatgg cggcatgcag cgctggttcg
      481 agccgatggc gacgacggtc gtcgcgcaac cggtctggtc gaaattgctg ctggcgctgg
      541 cgcacacatg cacggcgcgg atgccgtcgc cgcgctggtg catcgaggcg caccaattcc
      601 gcatcgacac cgtcgacggt ctcggtcggc cgacgccgga aggcgcgcat cgcgatggcg
      661 tggatttcgt cgcggtgctg ctggtcgcgc gcgcgggcat caagggcggc gagacacgcg
      721 tcttcgaagc ggcgggcccg gacggccagc gcttcaccct cggcgagccg tggtcgatgc
      781 tgctgctgga cgatgcgcgg gtgatccacg agtccacgcc gatccagccg ctgggcgact
      841 acggatatcg cgatacgctg gtgctgacct tccgcgccgc cggcttccag ggcgaggtct
      901 gaaggttaca attgcaacta ttcaatcgca tcgtttcgag gttgccatga agctgggttc
      961 attgaaggaa ggcgggcgcg acggcaccct gatcgtggtc tcgcgcgatt tgacccgcgc
     1021 ggtgcgcgcc accggcatcg cgccgaccct gcagcgcgcg ctggaagact gggaaagcgc
     1081 tgcaccgcgg ctcaatgcgc tgtccgacgc gctcaatgac ggcagcgccg acggtgtgtt
     1141 cgaccttgac ctgcaggcgc tggcggcccc gctgccgcgc gcctacgaat tcgtcgacgg
     1201 cagtgcctac ctgccgcatg tggcgcgcgt gcgccgtgcg cgcggggccg aggtgccgga
     1261 gagtttctat gtcgatccgc tgatgtacca ggcggtcagc gccggcttct acgggccgcg
     1321 cgacgcggtg aaggtggtca gcgaggacta cggcatcgac ctcgaggccg agatcgtgat
     1381 cgtcaccgac gacgtgccga tggcggtcac gtcggaagcg gccgcgggcc acatccagct
     1441 ggtcggcctg gtcaacgacg tctccctgcg caacctgatc ccgggcgagc tggccaaggg
     1501 cttcggcttc ctgcagtcca agccgcgtag cgcgctgagc ccggtgttcg tgactccgga
     1561 cgagctcggc agccactggc agggcaacaa gctgcacctg ccgctgctga cccatgtcaa
     1621 cggcaggtgg ttcggcgcgc cggaggccgg ggtcgacatg cagttcgact tcgcccagct
     1681 ggtggcgcat gcggcgaaga cgcggccgct gtcggccggc accatcgtcg gctcgggtac
     1741 ggtggcgaac gaggacaccg cgcttggcgc gtcctgcttc gccgagcagc gcacggtgga
     1801 ggccctgcgc gacggcaagc cgtcgacgcc gttcatggcc ttcggcgaca cggtacgcat
     1861 cgaggtcctg gaccgcgacg gcaccagcat cttcggcgcg atcgagcagc ggatcgagcg
     1921 ccagccgcag ccctgacccc ggcgcggggc acaggcggtc gcgggcagcg ggcgagcggg
     1981 cttccccccg gcggccgcgg ccgctaaggt gcccggacgg gcgaccgagg ggacgcgatg
     2041 aacgaggcgc tgcggcttta ctcctactgg cgctccagcg cggcctatcg ggtgcgcatc
     2101 ggcctcaacc tgaaggggct ggcctacgac atcgtgccgg tgcacctgct gcgcgacggc
     2161 ggccagcagc attcgccgga ataccgcggc atcaatccgc agcagctggt cccggtgctg
     2221 ggccacggcc atcgccggct cgggcagtcg ctggcgatcc tggaatacct cgacgagatg
     2281 tggccggcgc cgccgctgct gccgagcacc gcgcgcgaac gccagcgggt gcgtgcgctg
     2341 tcgatgctgg tggcctgcga catccatccg ctcaacaacc tgcgggtcct gcagtacctg
     2401 gagcaggagt ggaacgtgcc gcagcccgag cgcgacgaat gggtgcggca ctggatcgtc
     2461 ga
//
LOCUS       DAORUK010000227         2449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_64430_length_2449_cov_20.500000, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000227 DAORUK010000000
VERSION     DAORUK010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64430_length_2449_cov_20.500000"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="PK818_10620"
     CDS             <1..1232
                     /locus_tag="PK818_10620"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HQY83221.1"
                     /translation="LAFAARAGERRMALKELQVMVVEDHGFQRRIAVRLLAELGVVKV
                     LEGADGLHALEVLRLQAAVPDVVLVDLDMPGMDGIECIGHIAQERLARAVVVVSALDP
                     ALLNTVQTMARAYGLRVLGSVEKPLTRDKLESTLNRIDQHAGEEPDDAVAEFGIEALQ
                     EALQAGEITAWFQPQVEFANGKVIGVEALARWERPDGSLVRPCHFVPMMEREGLANAL
                     TDRMLDEACRWKQRWDHGGHRLKLSVNVSAVALADPSSADRYQAIVESHDIEPEEVIL
                     EITESSVMSDAARGLGLLARLRLKGFGLSIDDFGTGYSSLAQLSQIPFTELKIDKGFV
                     IDAHAQPRKRAVVEASLDLARKLGLSTVAEGVETIEDWQMLAELGCDVAQGYLIARPV
                     PGSELQAVVNRWRRPAH"
     gene            1240..>2449
                     /locus_tag="PK818_10625"
     CDS             1240..>2449
                     /locus_tag="PK818_10625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQY83222.1"
                     /translation="MAGRFDTARLSIRHQLWGLFGLFLLTGALVLVLDEVSQYYTQRT
                     MLAMKDDVLDGMRRIRRLSDAYSLDLVDTTFKARNALVEWEQAVAVVDRAQATIEAEW
                     TALQGTEFVGDDRALLEQALLARPRADAAAQELRKALVAKDVRALGRFADQELYPAID
                     PLTERLQVLAERGQFRADALVQAQITQGTWVSRTRVALSLLCFVLVALFGRRILRNGY
                     RGVESLHALSHRMAQHDYDATPHYRPTGELGDVLDSFLRMRDHVRRIETQLTNQLMSN
                     DRVRVALERREHFQRLLLEAAQTAIFAVDDDGVFSQVNPFAEQMLGWPAGSLLGREKL
                     DVILDADALKALSHHLSEAYGQPVAADWTALRAMAQHREPPREFVLHHQRGRTLPVLL
                     ALSAMRDDTGA"
BASE COUNT          374 a          796 c          911 g          368 t
ORIGIN      
        1 gcttggcgtt cgccgcacgc gctggggagc gccgcatggc cctgaaggaa ttgcaggtga
       61 tggtggtcga ggatcacggc ttccagcgcc ggatcgcggt acggctgctt gccgagctgg
      121 gggtggtcaa ggtgctggaa ggcgccgacg gcctgcatgc gctggaggtg ctgcggctgc
      181 aggcggcggt gccggacgtg gtgctggtgg acctggacat gccggggatg gacggcatcg
      241 agtgcatcgg ccacatcgcc caggagcgcc ttgcgcgcgc ggtggtggtg gtcagtgcgc
      301 tggacccggc gttgctcaat accgtgcaga cgatggcccg cgcctatggc ctgcgcgtgc
      361 tcggcagcgt cgagaagccg ctgacccgcg acaagctgga atccaccctc aaccggatcg
      421 accagcatgc cggcgaggag ccggacgacg cggtggccga attcggcatc gaggcgctgc
      481 aggaggcgct gcaggccggc gagatcaccg cctggttcca gccgcaggtg gaattcgcca
      541 acggcaaggt gatcggggtc gaggcgctgg cgcgctggga gcgccccgac ggcagcctgg
      601 tgcggccctg ccacttcgtg ccgatgatgg agcgcgaagg cctggccaat gcgctgaccg
      661 accgcatgct cgacgaagcc tgccggtgga agcagcgctg ggaccatggc gggcaccggc
      721 tgaagctgtc ggtcaacgtc tcggcggtgg cgctggccga cccgtcgtcg gccgaccgct
      781 accaggcgat cgtggagagc cacgacatcg agccggagga agtgatcctg gagatcaccg
      841 agagctcggt gatgtcggat gcggcgcgtg gcctgggcct gctggcgcgg ctgcggctga
      901 aggggttcgg gctgtcgatc gatgatttcg gcaccggcta ttcctcgctc gcgcagctct
      961 cgcagatccc gttcaccgaa ctcaagatcg acaagggctt cgtgatcgac gcccacgcgc
     1021 agccgcgcaa gcgggcggtg gtggaggcca gcctggacct ggcgcgcaag ctcgggctgt
     1081 cgaccgtggc cgagggcgtg gagaccatcg aggactggca gatgctggcc gagctcggat
     1141 gcgacgtcgc ccagggctac ctgatcgcgc ggccggtacc cggcagcgag ttgcaggcgg
     1201 tggtcaaccg ctggcggcgg ccggcccatt aagcacgcaa tggcgggccg cttcgacacc
     1261 gcgagactca gcatccgcca ccagttgtgg ggcctgttcg gcctgttcct gctgaccggt
     1321 gcgctggtac tggtgctgga cgaggtcagc cagtactaca cgcagcgcac gatgctggcg
     1381 atgaaggacg acgtgctgga cggcatgcgc cgcatccgcc gcctgtccga tgcctacagc
     1441 ctggacctgg tcgacaccac cttcaaggcg cgcaatgcgc tggtggagtg ggagcaggcg
     1501 gtggccgtgg tggatcgcgc gcaggccacc atcgaggccg aatggaccgc gctgcagggc
     1561 actgaattcg tcggcgacga ccgtgccctg ctggagcagg cgctgctggc gcgcccgcgc
     1621 gccgacgcgg cggcgcaaga attgcgcaag gcgctggtgg ccaaggacgt gcgcgcgctc
     1681 ggccgcttcg ccgaccagga gctttacccg gcgatcgatc cgctgaccga gcgcctgcag
     1741 gtgctggcgg aacgggggca attccgcgcc gatgcgctgg tgcaggcgca gatcacccag
     1801 ggcacgtggg tcagccgtac ccgcgtcgcg ctctcgttgc tgtgcttcgt gctggtcgcg
     1861 ctgttcggcc ggcgcatcct gcgcaacggc taccgcgggg ttgagagcct gcatgcgctg
     1921 tcgcaccgga tggcccagca cgactacgac gccacgccgc actaccgtcc caccggcgag
     1981 ctgggcgatg tgctggacag cttcctgcgc atgcgcgacc acgtgcggcg gatcgagacc
     2041 cagctgacca accagctgat gagcaacgac cgcgtgcggg tcgcgctgga acgccgcgaa
     2101 catttccagc ggctgctgct ggaggcggcg cagaccgcga tcttcgcagt cgacgatgac
     2161 ggcgtgttct cgcaggtgaa cccgttcgcc gagcagatgc tgggctggcc ggcaggctcg
     2221 ctgctgggcc gcgagaagct cgacgtgatc ctggacgcgg acgcgctgaa ggcgctctcg
     2281 caccacctca gcgaggccta cgggcaaccg gtcgccgccg attggaccgc gttgcgggcg
     2341 atggcccagc accgcgagcc gccgcgcgag ttcgtgctcc accaccagcg cggccgcacc
     2401 ctgccggtgc tgctggcgct gtcggcgatg cgcgacgata ccggcgcga
//
LOCUS       DAORUK010000228         2426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_65426_length_2426_cov_17.867566, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000228 DAORUK010000000
VERSION     DAORUK010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65426_length_2426_cov_17.867566"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..1151
                     /locus_tag="PK818_10630"
     CDS             207..1151
                     /locus_tag="PK818_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HQY83223.1"
                     /translation="MLERVRERCRVRHYSIRTEQAYVAWIWRFIRANAGLHPRVLGKP
                     EIETFLTRLAMEGEVAASTQNQALSALLFLYREVLDVELPWLDEVVRAKRPRRVPVVL
                     SREEVGRLLAMLDGRAWLQAALLYGSGMRLMECLRLRVKDVDFERSEITVRAGKGNKD
                     RRVPLPKRLREPLVLAVARVGLLHAQDRASGLDGVWLPHALARKYPQAGFELGWQYVF
                     PAEKPSRDPRSGLLRRHHVDEATLQRAVQAARRKAGIVKPATCHTLRHSFATHLLEAG
                     QDIRTIQELLGHKDVATTQIYTHVLNRGPHAVLSPLDR"
     gene            complement(1188..1889)
                     /gene="nfi"
                     /locus_tag="PK818_10635"
     CDS             complement(1188..1889)
                     /gene="nfi"
                     /locus_tag="PK818_10635"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465601.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HQY83224.1"
                     /translation="MTDALPAWTGDTAALRAVQDELARKVVIRDDFPKPLRTVAGFDV
                     GFEDDGATTRAAAVLLDADTLEVIDQQVARAPTRMPYIPGLLGFRELPALLEALAMLE
                     RAPDLAFIDGHGIAHPRRLGIAAHFGVATNLPCIGVARKILVGEARMALHEMRGAFTP
                     LRDGREQIGWLLRSKPGCLPLLVSPGHRVAMPSAPELVMRFVTRYRLPEPTRLADRLA
                     SPSEDTSLTADEHAG"
     gene            complement(1886..>2426)
                     /locus_tag="PK818_10640"
     CDS             complement(1886..>2426)
                     /locus_tag="PK818_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQY83225.1"
                     /translation="RMRLSLLLACSLLAACTGAVRMAPAAADTTTTTFIVVRHAEKVD
                     ASRDPDLSPAGRARAQALAESLEGRELVAIHATEFKRTGQTVAPTASAHALPVGTYAS
                     TQAAAEFAAHLKAAHPRGIVLIAGHSNTVPGIVAALCNCETAPMADHEYDRLSSIRVD
                     ADGRARLDVTRYGAASPTP"
BASE COUNT          348 a          709 c          965 g          404 t
ORIGIN      
        1 cgggatgaag ctcgagtatg gggcgtactg ggagaggcta ccagcgggga aatcctactt
       61 cgaatcaatg ctttggacta tgaccctggc gccgatcctg cggcatcttg cttggcaagg
      121 cggcgatagt gggatgtagc gtggacaaga cctctccatg gcatggcaag caagggcttt
      181 cgcacaatcc ggggcgcgtt cccgggctgc ttgagcgtgt gcgggagcgt tgtcgggtgc
      241 ggcattacag cattcgcacg gagcaggcct acgttgcgtg gatctggcgg ttcatccgtg
      301 ccaatgccgg cttgcatccg cgtgtcctcg gaaagcctga gatcgagacg tttctgaccc
      361 gcctggcgat ggagggcgaa gttgcagctt ccacccagaa ccaggcgctg tcggcgctgc
      421 tgttcctgta ccgggaggtg ctggacgtgg agttgccgtg gctcgacgag gtggtgcggg
      481 cgaaacgccc gcgtcgggtg ccggttgtgc tgtcgaggga ggaggtcggt cggttgctgg
      541 cgatgctgga tggtcgcgcg tggttgcagg ctgcactcct gtacggttct ggcatgcggc
      601 tgatggagtg cctgcgcctg cgggtcaagg acgtggactt cgagcgatcc gagatcacgg
      661 tgcgggcagg caagggaaac aaggatcggc gggttccgtt gccgaagcgt ttgcgggagc
      721 cgctggtgct ggcggtcgcc cgcgtaggtt tgctccacgc gcaggaccgg gcgtcgggat
      781 tggatggtgt ctggctgccg cacgcgctgg cgcgcaagta tccgcaggcg gggttcgagc
      841 ttgggtggca gtacgtcttt cccgcggaga agccctcgcg agatccccgc agcggcctct
      901 tgcgccggca ccacgtcgac gaggcgacct tgcagcgtgc ggtccaggca gcaaggagga
      961 aggctggaat cgtcaagccg gcgacttgcc acacgctgcg gcattcgttt gcgacgcatt
     1021 tgctcgaggc gggtcaggac atccgcacga tccaggaatt gctggggcac aaggacgtgg
     1081 cgacgaccca gatctatacg cacgtgctga accgcgggcc gcatgcggtg ctgagtccgc
     1141 tggatcgcta gcggggcttc gcgtgcacgt ggcgcacgcc gcgaggttca gccggcgtgt
     1201 tcgtcggcgg tcaggctggt gtcttcgctg ggcgaggcca ggcgatcggc aagcctggtt
     1261 ggttccggca ggcggtagcg ggtgacgaag cgcatcacca gttccggcgc ggacggcatc
     1321 gcgacccggt ggccgggcga aaccagcagc ggcaggcagc cgggcttgct gcgcagcagc
     1381 cagccgatct gttcgcggcc gtcgcgcagc ggggtgaagg cgccgcgcat ctcgtgcagg
     1441 gccatccgcg cttcgccgac caggatcttc ctggcgacgc cgatgcaagg caggttggtg
     1501 gcgacgccga agtgcgcggc gatgcccagc cggcgcgggt gggcgatgcc gtggccgtcg
     1561 atgaaggcga ggtccggcgc gcgctcaagc atcgccagcg cctcgagcag ggcgggcagt
     1621 tcgcggaagc cgagcaggcc ggggatgtag ggcatccggg tgggcgcgcg cgcgacctgc
     1681 tggtcgatca cctccagcgt gtcggcgtcg agcagcaccg cggcggcgcg ggtggtggcg
     1741 ccgtcgtcct cgaagccgac gtcgaagccg gcgacggtgc gcagcggctt cgggaaatcg
     1801 tcgcggatga cgaccttgcg cgcgagttcg tcctgcacgg cgcgcagggc cgcggtatcg
     1861 ccggtccagg cggggagggc gtcggtcacg gggttggcga ggccgcgccg tagcgggtga
     1921 cgtccaggcg ggcgcggcca tcggcatcga cccggatgct gctgaggcgg tcgtactcgt
     1981 ggtcggccat cggcgcggtc tcgcagttgc acagcgcggc aacgatgccc ggcacggtgt
     2041 tgctgtggcc ggcgatgagg acgatgccgc gcggatgcgc ggccttcagg tgggcggcga
     2101 attcggcggc cgcttgcgtg gacgcatagg tgccgaccgg aagcgcgtgg gcgcttgcag
     2161 tgggtgccac ggtctgcccg gtgcgcttga actcggtggc atggatcgcg acgagttcgc
     2221 ggccctcgag cgattccgcg agcgcttgcg cgcgggcgcg gccggcgggg ctgaggtcgg
     2281 ggtcgcggct ggcatcgact ttctccgcgt ggcggacgac gatgaaggtc gttgtcgtgg
     2341 tgtccgcggc ggcgggggcc atgcggaccg cgccggtgca cgcggcgagc agggaacagg
     2401 caagcaggag ggataggcgc atgcgc
//
LOCUS       DAORUK010000229         2425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_65476_length_2425_cov_23.106751, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000229 DAORUK010000000
VERSION     DAORUK010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65476_length_2425_cov_23.106751"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PK818_10645"
     CDS             <1..632
                     /locus_tag="PK818_10645"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HQY83226.1"
                     /translation="FVGHDLNALRAFCQRAILLEDGRMAMLGAPEEVIEEYLFRVHAK
                     AARDGQAKPIRIAEGFRSEDGGVLGLSLQGGARHLALNCGDPLEVDIDLEVQAALADA
                     ACIIDILDERGVPLSGRRIRLPESADGPQRLRLRVAFRAELARGIYRVRARLVQAPDR
                     HQHHRRVLCRYESDLSFEVIDNSIERFSGLFPLPAEVVVQRLGTDDHGA"
     gene            616..1383
                     /locus_tag="PK818_10650"
     CDS             616..1383
                     /locus_tag="PK818_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HQY83227.1"
                     /translation="MTTAPEPRIRFLLGGVQKAGTSALARYLSTHPRLRLPIDKEAHV
                     FDAPDFDEGWTPEQVDRRYADRFDDAGAGAIHGDATPFYVMHPRVVARIARYNPGMRW
                     IVLLRDPGERALSHFHMERQRGLEGWPLWPALLLERWRLRGHHEDLQRGSPLRRHGYR
                     LRGDYARQLDVLYSHFPREQVLLLRSDRLREQPAACVREACAFLGVEPPAADASYPPV
                     FVGDYPAPGHWTRGLLRWLLRHERRALRTRYGIAFDD"
     gene            complement(1391..>2425)
                     /locus_tag="PK818_10655"
     CDS             complement(1391..>2425)
                     /locus_tag="PK818_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HQY83228.1"
                     /translation="GVHQGFEYSRTHNPTRFAYERCVASLEGGSRGFAFASGLAATST
                     LLELLDSGDHVIAMDDLYGGSYRLFERVRRRSAALDFSFVDLTDPAKFEAAITPKTKL
                     VWIETPTNPLLKIVDIAAIAAIAKARGLLVAVDNTFASPILQRPLEHGADIVMHSATK
                     YLNGHSDMVGGMLVVGDNDELGEQLAFLQNSVGAVQGPFDSFLALRGLKTLHLRMQAH
                     CANAQALAEWLQTHDAVSDVIYPGLASHPHHALAARQMDGFGGMLSIRLKGGFEAAKT
                     FCERLELFTLAESLGGVESLVNHPAVMTHASIPPARRAELGIGDELVRLSVGIEALDD
                     LRADVAQALA"
BASE COUNT          352 a          861 c          844 g          368 t
ORIGIN      
        1 tgttcgtcgg ccacgacctc aacgccctgc gcgcgttctg ccagcgcgcg atcctgctcg
       61 aggacgggcg catggcgatg ctgggcgcac ccgaggaagt gatcgaggaa tacctgttcc
      121 gggtgcacgc caaggcggcg cgcgacggcc aggccaagcc gatccggatc gccgagggct
      181 tccgcagcga ggacggcggc gtgctcggcc tgtcgctgca gggcggcgcg cgccacctgg
      241 cgctcaactg cggcgatccg ctggaggtcg acatcgacct cgaggtgcag gccgcgctgg
      301 cggatgccgc ctgcatcatc gacatcctcg acgagcgcgg ggtcccgctc agcggccgcc
      361 ggatccgcct gccggagtcc gccgacggcc cgcagcggct gcgcctgcgg gtcgccttcc
      421 gcgccgaact ggcgcgtggc atctatcgcg tgcgcgcgcg cctggtgcag gcgccggacc
      481 gccaccagca ccatcgccgg gtgctgtgcc gctacgaatc ggacctgtcg ttcgaggtca
      541 tcgacaattc catcgagcgc ttcagcgggc tgttcccgct gccggcggag gtggtggtgc
      601 agcggctggg cacggatgac cacggcgcct gagccgcgca tccgcttcct gcttggcggt
      661 gtgcagaagg ccggcaccag cgcactggcg cgctacctgt ccacccaccc acgcctgcgg
      721 ctgccgatcg acaaggaagc gcatgtgttc gatgcaccgg acttcgacga aggctggacg
      781 ccggaacagg tcgaccggcg ctacgcggac cggttcgacg acgccggtgc aggcgccatc
      841 cacggcgatg ccaccccgtt ctacgtgatg cacccgcggg tggtcgcgcg gattgcccgc
      901 tacaacccgg gcatgcgctg gatcgtgttg ctgcgcgacc cgggcgaacg tgcgctctcg
      961 cacttccaca tggaacgcca gcgcggcctg gagggctggc cgctgtggcc ggccctgctg
     1021 ctggaacgct ggcggctgcg cggccaccac gaggacctgc agcgaggctc gccgttgcgc
     1081 cggcacggct accgcctgcg cggcgactac gcgcgccagc tggacgtgct gtattcgcat
     1141 ttcccgcgcg aacaggtgct gctgctgcgc agcgatcgcc tgcgcgaaca gccggcggcc
     1201 tgcgtacgcg aggcctgcgc gttcctcggg gtcgaaccac ccgcggcgga tgcgtcctac
     1261 ccgccggtgt tcgtcggcga ctatccggcg cccggccact ggacccgcgg cctgctgcgc
     1321 tggctgctgc gccacgagcg gcgcgctttg cgcacccgct acggcatcgc cttcgacgac
     1381 tgaggcacgc tcaggccagc gcctgcgcca cgtccgcgcg caagtcgtcg agcgcctcga
     1441 tgcccacgct caggcgcacc agctcgtcgc cgatcccgag ttccgcgcgg cgcgccggcg
     1501 ggatcgaggc gtgggtcatc accgccgggt ggttgaccag cgactccacc ccgcccagcg
     1561 actcggccag ggtgaacagc tcgaggcgtt cgcagaaggt cttcgccgct tcgaagccgc
     1621 ccttgaggcg gatcgacagc atgccgccga agccgtccat ctgccgcgcc gccagcgcat
     1681 ggtgggggtg cgaagccagc cccgggtaga tgacgtcgga gaccgcatcg tgggtctgca
     1741 accattcggc cagcgcctgc gcattcgcgc aatgcgcttg catgcgcagg tgcaacgtct
     1801 tcaggccgcg cagcgcgagg aagctgtcga acggaccctg caccgcaccg acggaattct
     1861 gcaggaacgc cagttgctcg ccgagttcgt cgttgtcgcc gaccaccagc atgccgccga
     1921 ccatgtccga atggccgttg aggtacttgg tcgccgaatg catgacgatg tcggcgccat
     1981 gttccagcgg ccgctgcagg atcggcgagg cgaaggtgtt gtcgaccgcc accagcagcc
     2041 cgcgcgcttt cgcgatggcg gcgatcgcgg cgatgtcgac gatcttcagc agcgggttgg
     2101 tcggggtctc gatccacacc agcttcgtct tcggcgtgat cgccgcttcg aacttcgccg
     2161 gatcggtcag gtcgacgaag ctgaagtcca gggcggcgct gcgccggcgc acgcgctcga
     2221 acaggcgata gctgccgccg tagagatcgt ccatcgcgat gacgtggtcg ccgctgtcca
     2281 gcaactccag cagggtcgag gtcgccgcca gcccggaggc gaacgcgaag ccgcggctgc
     2341 cgccttccag cgaggccacg caacgctcgt atgcgaagcg ggtggggttg tgcgtgcgcg
     2401 aatactcgaa gccctggtgc acgcc
//
LOCUS       DAORUK010000230         2393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_66903_length_2393_cov_21.835757, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000230 DAORUK010000000
VERSION     DAORUK010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66903_length_2393_cov_21.835757"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..498)
                     /locus_tag="PK818_10660"
     CDS             complement(49..498)
                     /locus_tag="PK818_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HQY83229.1"
                     /translation="MNRMIRTTVLALALWPALALAQAQPAAAPLPAWEQLTPAQRNAL
                     VAPLRERWNANPGERARMLERAQRWHGMPREQRDRARHGLQRWEHMTPEQRTEARALF
                     HAMRGMDKEQRTAFLAQWQQKTPQQKAEWLKAHPAPVHGEKDRDDRD"
     gene            complement(502..858)
                     /locus_tag="PK818_10665"
     CDS             complement(502..858)
                     /locus_tag="PK818_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83230.1"
                     /translation="MNSHHDDRFDAALRARHQASLDRLSPRASAQLAQRRNAALRGET
                     VRSGNGFRYAAAGFAALGALAIGLQFGMPTAPTSTATVAGSVAASTTTAATMLDEDPE
                     FYAWLASPDAQLVAME"
     gene            complement(855..1412)
                     /locus_tag="PK818_10670"
     CDS             complement(855..1412)
                     /locus_tag="PK818_10670"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQY83231.1"
                     /translation="MSVEPNLQDPGAAAALPATLEEFLAGIDRRAFRFAEIALRSRED
                     ALDAVQDAMMKMLGYRDRTPAEWTPLFWSILRNRITDMQRRGLFRLRWMLPGSSNDDG
                     DPIDWADDGPDPSRRNDSREAWARISTVLGRLPARQREAFTLRVLEELDVADTARAMG
                     CSEGSVKTHLSRAREAMQKQLEEFR"
     gene            1605..1904
                     /locus_tag="PK818_10675"
     CDS             1605..1904
                     /locus_tag="PK818_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HQY83232.1"
                     /translation="MGDGFVALYIFMLAAIAGHVIISRVPVILHTPLMSGSNFIHGIV
                     LIGAMVVLGHADTTVEKALGFVAVLLGAGNAAGGYVVTERMLDMFKSSRPAGGKA"
     gene            1901..>2393
                     /locus_tag="PK818_10680"
     CDS             1901..>2393
                     /locus_tag="PK818_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HQY83233.1"
                     /translation="MTILALVKLSYFVAATLFLLGLQRMASPKTARSGIQWAGVGMLI
                     ATIATFFLPGLHNLGLMAAAILIGVGLNWAWGKKVAITDMPQMVALFNGMGGGSAAAI
                     GAMELVRWSAAGQSSGPVPAVLAVVGALIGAVSLTGSVIAWAKLDGRMDKRYTFPGQQ
                     WFNA"
BASE COUNT          295 a          786 c          905 g          407 t
ORIGIN      
        1 ttgctggcga agacggtgtg gccggagcgc gcaccgccgg caagctgatc agtcccggtc
       61 gtcgcggtcc ttttcgccgt gcaccggcgc cgggtgcgcc ttcagccact cggccttctg
      121 ctgcggggtc ttctgctgcc attgcgcaag gaacgccgtg cgctgctcct tgtccatgcc
      181 gcgcatggcg tggaacagcg cgcgcgcctc ggtgcgctgc tccggcgtca tgtgttccca
      241 gcgctgcagg ccgtggcgcg cgcggtcgcg ctgctcgcgc ggcatgccgt gccagcgctg
      301 ggcgcgttcg agcatgcgtg cgcgctcgcc ggggttggcg ttccagcgtt cgcgcagggg
      361 cgcgaccagg gcattgcgct gggcaggggt gagctgctcc caggccggta gcggggcggc
      421 ggcgggttgc gcctgggcaa gcgcgagcgc gggccagagc gccagcgcga gcacggtggt
      481 gcggatcatt cggttcatcg cttactccat cgcgaccagc tgggcatcag gcgatgccag
      541 ccacgcatag aactcggggt cttcatccag catcgtggcc gcagtggtgg tggatgccgc
      601 cacgctgcca gcgacggtgg cggtgctggt tggtgccgtc ggcatgccga actgcaggcc
      661 gatggcgagc gcgcccagcg cggcgaagcc ggcggcggcg tagcggaagc cgttcccgct
      721 ccggaccgtt tcgccgcgca gggcggcgtt gcggcgctgc gcgagttgcg cgctggcgcg
      781 cggcgacagg cggtccagcg atgcctggtg gcgtgcgcgg agcgcggcat cgaagcggtc
      841 gtcgtggtgg ctgttcatcg gaattcttcc agttgtttct gcatggcttc gcgcgcgcgc
      901 gacaggtggg tcttgaccga gccttcgctg cagcccatcg cccgcgcggt gtcggcgacg
      961 tccagctcct ccagcacgcg cagggtgaag gcctcgcgct ggcgcgcggg cagcctgccc
     1021 agcaccgtgc tgatccgcgc ccaggcctcg cggctgtcgt tgcggcgcga cggatccggg
     1081 ccgtcgtcgg cccagtcgat cgggtcgccg tcgtcgttgc tgctgccggg gagcatccag
     1141 cgcaggcgga acaggccgcg ccgctgcatg tcggtgatgc ggttgcgcag gatgctccag
     1201 aacagcgggg tccattccgc cggggtgcgg tcgcggtagc ccagcatctt catcatcgcg
     1261 tcctgcaccg cgtccagcgc atcctcgcgg ctgcgcaggg cgatctcggc gaagcggaac
     1321 gcgcgcctgt cgatgccggc gaggaattcc tccagggtcg ccggcagcgc cgcggcggcg
     1381 ccagggtcct gcaggttggg ctcgacgctc acgcgtccaa gcttagccgg gtcgaacagc
     1441 agcatggggc cccgtgccgg gcatcggcat caccacgatc cgtggccggg gcgggattgc
     1501 gccggccgtg cagggaggaa cgcccggccg ccggcttggt tgacacgtgg cgcgccgccg
     1561 ctggccgtgt ttatgatgcg gacaggcaat cggggagagg catcgtgggc gacggtttcg
     1621 tggcgttgta catcttcatg ctggcggcga tcgccggcca cgtgatcatc tcgcgggtgc
     1681 cggtgatcct gcatacgccg ttgatgtcgg gttccaactt catccacggc atcgtcctga
     1741 tcggcgcgat ggtggtgctg ggacacgccg acaccaccgt ggagaaggcg ctcggcttcg
     1801 ttgcagtgct gctgggcgcc ggcaacgcgg ccggcggcta cgtggtcacc gaacgcatgc
     1861 tggacatgtt caagtcgtcc cggccggcgg ggggcaaggc atgaccatcc tcgcgctggt
     1921 caagctgagc tatttcgtcg ccgccacgct gttcctgctt ggcctgcagc gcatggccag
     1981 cccgaagacc gcgcgcagcg gcatccagtg ggcgggcgtg ggcatgctga tcgcgaccat
     2041 cgccaccttc ttcctgcctg ggctgcataa cctcgggttg atggccgccg ccatcctgat
     2101 cggcgtcggc ctgaactggg cgtggggcaa gaaggtcgcg atcaccgaca tgccgcagat
     2161 ggtggcgctg ttcaacggca tgggcggcgg ctcggcggcg gcgatcggcg cgatggagct
     2221 ggtgcgctgg tcggcggccg ggcaatccag cgggccggtg ccggcggtgc tggcggtcgt
     2281 cggtgcgctg atcggcgcgg tctcgctgac cggttcggtg atcgcctggg ccaagctcga
     2341 cgggcgcatg gacaagcgct acaccttccc cggccagcag tggttcaacg cgg
//
LOCUS       DAORUK010000231         2382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_67428_length_2382_cov_17.354963, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000231 DAORUK010000000
VERSION     DAORUK010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67428_length_2382_cov_17.354963"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PK818_10685"
     CDS             <1..624
                     /locus_tag="PK818_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQY83234.1"
                     /translation="DMKKLIAYSSISHMGFVTLGTFIAFALAREQGNLDAARLGLQGA
                     MVQMVSHGFISAAMFSCVGVLYDRVHSRMIRDYGGVANTMPWFAALFVLFAMANCGLP
                     GTSGFVGEFMVIVASFQANIWYALGAGLTLVLGAAYTLWLVKRVVFGEVANKNVAQLK
                     DLGAREAWVLGGYAAAVLLLGVWPKPLTDLMEAPIAALVQQLLLGKA"
     gene            638..2092
                     /gene="nuoN"
                     /locus_tag="PK818_10690"
     CDS             638..2092
                     /gene="nuoN"
                     /locus_tag="PK818_10690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HQY83235.1"
                     /translation="MFAATDLAALLPEAFMLGAICALLLIDLFIDQSRRGLTHFLAIT
                     SLVFAAILTLRTHGAAGGAVEPVRAFGGMFVRDGFGDLLKLAIHAVTIGVFVYAKPYL
                     KSHSLFQGEFYVLALTGVLGMMVLVSAGNLVVVYLGLELLALSSYALVALDRDSRLAS
                     EAAIKYFVLGALASGLLLYGMSMIYGATGTLDLAQIHAQVAVSYDRTLLAFGLVFIVV
                     GVAFKLGAAPFHMWVPDVYQGSPTAVTLFIAGAPKIAAFGMAYRLLEDGLAGLATDWQ
                     PMLTLLALLSLAIGNLVAIAQTNLKRMLAYSTISHIGFLLLGLINGTPAGLAAATFYA
                     ITYAITTVAAFGMIVLLSRSGFEAEEIEDFRGLNQRNGWYAMIMLMVMASLAGVPVWV
                     GFMAKFQVLKAAVESGHLWLAIAAGVFAVIGAFYYLRVIKVMYFDDPATTEPLALPND
                     MPFRFVLSANGLGLLALGILGGPLMAWCLEAVTR"
BASE COUNT          301 a          813 c          806 g          462 t
ORIGIN      
        1 gacatgaaga agctgatcgc ctattcgtcg atctcgcaca tgggcttcgt caccctgggc
       61 accttcatcg ccttcgcgct ggcgcgcgag cagggcaacc tagacgccgc gcgcctgggc
      121 ctgcagggcg cgatggtgca gatggtttcg cacggcttca tctcggcggc gatgttctcg
      181 tgcgtgggcg tgctctacga ccgggtccac agccgcatga tccgcgacta cggcggcgtc
      241 gcgaacacca tgccgtggtt cgccgcgctg ttcgtgctct tcgccatggc caactgcggc
      301 ctgcccggca ccagcggctt cgtcggcgag ttcatggtca tcgtggccag cttccaggcc
      361 aacatctggt acgcgctggg cgccggcctg accctggtgc tgggcgcggc ctacacgctg
      421 tggctggtca agcgcgtggt cttcggcgag gtcgccaaca agaacgtggc gcaactcaag
      481 gacctcggcg cgcgcgaggc ctgggtgctg ggcggctatg ccgcggcggt gctgctgctg
      541 ggcgtgtggc ccaagccgct gaccgacctg atggaggcgc cgatcgcggc gctggtgcag
      601 cagctcctgc tgggcaaggc ctgaggaatc acccgcgatg ttcgccgcca ccgaccttgc
      661 cgcgctcctg cccgaggcct tcatgctcgg cgcgatctgc gccctgctgc tgatcgacct
      721 tttcatcgac cagtcgcggc gcggcctgac ccatttcctc gcgatcacga gcctggtctt
      781 cgcggcgatc ctgaccctgc gcacgcacgg cgccgcgggt ggcgcggtcg agccggtgcg
      841 cgccttcggc ggcatgttcg tgcgcgacgg cttcggcgac ctgctcaagc tcgcgatcca
      901 cgcggtcacc atcggcgtgt tcgtctacgc caagccgtac ctcaagtcgc actcgctgtt
      961 ccagggtgag ttctacgtcc tggcgctgac cggcgtgctc ggcatgatgg tgctggtttc
     1021 ggctggcaac ctggtcgtgg tctacctcgg gctcgagctg ctggcgctgt cctcgtatgc
     1081 actggtcgcc ctggatcgtg attcgcgcct ggcctcggag gccgcgatca agtacttcgt
     1141 gctcggcgcg ctggcctcgg gcctgctgct gtacggcatg tcgatgatct acggcgccac
     1201 cggcacgctc gacctggcgc agatccacgc ccaggtcgcg gtgtcctacg accgcacgct
     1261 gctggccttc ggcctggtgt tcatcgtggt cggcgtggcc ttcaagctcg gcgcggcgcc
     1321 gttccacatg tgggtgcccg acgtctacca gggctcgccc accgcggtga cgctgttcat
     1381 cgcgggcgcg cccaagatcg cggccttcgg catggcctac cggttgctcg aggacggcct
     1441 cgcgggcctc gcgaccgact ggcaaccgat gctgaccctg ctggcgctgc tgtcgctggc
     1501 gatcggcaac ctggtcgcga tcgcgcagac caacctcaag cgcatgctgg cctactcgac
     1561 catctcgcac atcggcttcc tgctgctggg cctgatcaac ggcacgccgg ccggccttgc
     1621 cgccgcgacc ttctacgcga tcacctacgc gatcacgacg gtcgcggcct tcggcatgat
     1681 cgtgctgctg tcgcgctcgg gcttcgaggc cgaggagatc gaggacttcc gcgggctcaa
     1741 ccagcgcaac ggctggtacg cgatgatcat gctgatggtg atggcctcgc tggccggcgt
     1801 gccggtctgg gtcggcttca tggccaagtt ccaggtgctc aaggccgcgg tggaatcggg
     1861 ccacctgtgg cttgcgatcg ccgccggcgt gttcgccgtg atcggcgcct tctactacct
     1921 gcgcgtgatc aaggtgatgt acttcgacga tcccgccacc accgagccgc tggcgttgcc
     1981 caacgacatg ccgttccgct tcgtgctgtc ggccaacggg ctgggcctgc tggccctggg
     2041 gatcctgggt gggccgctga tggcctggtg cctcgaggcc gtgacgcgct agtggcggca
     2101 ggtccggcgc tgtaggagcc cactccgtgg gcgaccgagg acgcgggttg cctggaagtg
     2161 gcgatgcggc ggttcgcaat cgcgcggtcg cccacggagt gggctcctac gggattgcgg
     2221 gaattttcgt cgctgggcgg ctcggcatcc ccgcgcctac cactgcccgt gccaacattc
     2281 ggggttgatc ttccccctag ccgcccctat gattcgcgtc cgcctgatgc ggggtggagc
     2341 agtctggcag ctcgtcgggc tcataacccg aaggtcgtag gt
//
LOCUS       DAORUK010000232         2376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_67696_length_2376_cov_18.676433, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000232 DAORUK010000000
VERSION     DAORUK010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67696_length_2376_cov_18.676433"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PK818_10695"
     CDS             complement(<1..226)
                     /locus_tag="PK818_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQY83236.1"
                     /translation="MPIYAFACDACGHGFDRLQKLSDADPTVCPACGAEGAVRRQLTA
                     PQFRLSGGGWYETDFKKDGDRKRNLAGEGGE"
     gene            367..1071
                     /locus_tag="PK818_10700"
     CDS             367..1071
                     /locus_tag="PK818_10700"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HQY83237.1"
                     /translation="MQTRPLLAAVVIALGLAAAGWFASQGMAHLRTADRYVTVKGSAE
                     KIVDADLVVWPLSQTVGGNELGAVQAQLDANTQTIRSFLTTAGFKDDEIVVSPPRLED
                     RWAYSYGDNRPPERYRYSNTVTLRSSRVAEALAALRRSGDIVARGVMLNTDEGGGPDF
                     DYTKLNDIKPALIAEATSNARKSAEQFAKDSGARIGGIRSANQGVINIENRDAGSPQI
                     KKIRVVTTVEYFLKGE"
     gene            complement(1150..>2376)
                     /locus_tag="PK818_10705"
     CDS             complement(1150..>2376)
                     /locus_tag="PK818_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HQY83238.1"
                     /translation="FLDNVTTHILHLHDGTAKLYAGNYTDFERQRAEHLRQQQIAHQK
                     EQVERAHLQSFIDRFKAKASKARQAQSRMKRLAKLAGTEAVRAERSLHITLPEPLKLP
                     NSLLSLHDADCGYGAAARILANVNFGLEAGDRIGLLGPNGAGKSTLVKTLVGELAPMT
                     GERKAHPDLRIGYFAQHTVESLVFGSTPLDHLRKLAPDVANQELRDFLGRWNFPGERA
                     FEVIDTFSGGEKARLALALLAYRRPNVLLLDEPTNHLDLDMREALAEALSDFDGAIVM
                     VSHDRHLIGLVCDEYWRVAGGKVEPFDGDLDEYAAWLRSRPGSDNPKTPAPKAQPAIA
                     AAPARPAAKVNPHKLQKAEALVAELEAKLAGIDTQLADPKVYVDAARVAGLGREQASL
                     RTQLEAAEAGLLAMYD"
BASE COUNT          353 a          816 c          821 g          386 t
ORIGIN      
        1 cttcgccacc ttccccggcc aggttgcgct tcctgtcgcc gtccttcttg aagtcggtct
       61 cgtaccagcc accgccggag aggcggaatt gcggggcggt cagctggcga cgcaccgcgc
      121 cctcggcccc gcaggcgggg catacggtgg ggtcggcatc ggacagcttc tgcaggcggt
      181 cgaaaccgtg gccacaggcg tcgcaggcga aggcgtaaat cggcatcggg gcattccgca
      241 aagtccaggc gccgattgtg agggccgcgg ccggcgtttc aagggcggcg tccgcgcgtc
      301 cggcattccc gctacgtttc gttactggcg ggtatcgtcc ttccgaatcc gcccaggaac
      361 cccaccatgc aaacccgccc cctgcttgcc gccgtcgtca tcgccctcgg cctggccgcc
      421 gccggctggt tcgcctcgca gggcatggcc cacctgcgca ccgccgaccg ctacgtcacc
      481 gtcaagggtt cggcggagaa gatcgtggat gccgacctgg tggtctggcc gctgtcgcag
      541 accgtcggcg gcaacgagct cggcgcggtg caggcgcaac tggacgccaa tacccagacc
      601 atccgcagct tcctgaccac ggccggcttc aaggatgacg agatcgtggt cagcccgccg
      661 cggctggaag accgctgggc gtattcgtat ggcgacaacc gcccgccgga acgctaccgc
      721 tactcgaaca cggtgaccct gcgcagcagc cgggtcgccg aggcgctggc cgcgctgcgc
      781 cgcagcggcg acatcgtggc gcggggggtg atgctcaata ccgacgaggg cggcggcccc
      841 gacttcgact acaccaaact caacgacatc aagcccgcgc tgatcgccga ggccaccagc
      901 aacgcacgca agtcggccga gcagttcgcc aaggactccg gcgcgcgcat cggcggcatc
      961 cgcagcgcca accagggcgt gatcaacatc gagaaccgcg acgccggcag cccgcagatc
     1021 aagaagatcc gggtggtgac gacggtggag tacttcctga agggtgagtg aggccgcacg
     1081 caaaccactg gtttgcggcc tcacgaacgc ccggaggctg tgcctggggg ccggtcaaac
     1141 tcgcacttct cagtcgtaca tcgccaacag gcccgcttcg gcggcttcca gctgcgtgcg
     1201 caggctcgcc tgctcgcggc cgaggccggc cacgcgcgcc gcatccacgt agaccttggg
     1261 gtcggcgagc tgggtgtcga tgccggccag cttggcttcc agttcggcga ccagcgcttc
     1321 ggccttctgc agcttgtgcg ggttgacctt ggcggctggc ctggccggcg ccgcggcgat
     1381 cgccggttgc gccttggggg ccggggtctt cgggttgtcg ctgccgggcc gcgagcgcaa
     1441 ccacgccgcg tattcgtcga ggtcgccgtc gaagggttcg accttgccgc cggcaacccg
     1501 ccagtactcg tcgcagacca ggccaatgag atgccggtcg tggctgacca tcacgatggc
     1561 gccgtcgaaa tcggacaggg cctcggccag cgcctcgcgc atgtcgaggt cgaggtggtt
     1621 ggtcggttcg tccagcagca gcacgttcgg ccgccggtac gccagcagcg ccaatgcgag
     1681 gcgcgccttc tcgccgccgc tgaaggtgtc gatgacctcg aacgcgcgct cgccggggaa
     1741 attccagcgc cccaggaaat cgcgcagctc ctggttggcc acgtccggcg cgagcttgcg
     1801 caggtggtcc agcggggtgc tgccgaacac cagcgactcc accgtgtgct gggcgaaata
     1861 gccgatgcgc aggtccgggt gcgccttgcg ctcgccggtc attggcgcga gttcgcccac
     1921 cagggtcttg accagggtcg acttgccggc accgttcggg cccagcaggc cgatgcggtc
     1981 gccggcctcc aggccgaagt tcacgttcgc caggattcgc gccgcggcgc cgtagccgca
     2041 atcggcatcg tgcagggaca gcagcgaatt cggcagcttc agcggttccg gcagggtgat
     2101 gtgcagcgag cgctcggcgc gcaccgcctc ggtgccggcc agcttggcca gccgcttcat
     2161 ccgcgactgc gcctgcctgg ccttgctggc cttggccttg aagcggtcga tgaacgactg
     2221 caggtgcgca cgctccacct gttccttctg gtgcgcgatc tgctgctggc gcaggtgctc
     2281 cgcgcgctgg cgttcgaagt cggtgtagtt gccggcgtac agcttcgcgg tgccgtcgtg
     2341 caggtgcagg atgtgggtgg tgacgttgtc gaggaa
//
LOCUS       DAORUK010000233         2367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_68127_length_2367_cov_20.536765, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000233 DAORUK010000000
VERSION     DAORUK010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68127_length_2367_cov_20.536765"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="PK818_10710"
     CDS             <1..1071
                     /locus_tag="PK818_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83239.1"
                     /translation="QMGGNGLEHQRSSENGVGTDYFTGWKEKQGFTDLLAHEFVHSWN
                     GKYRRGKDLWVPNLNTPMQDSLLWVYEGQTQYWGNVLAARSGMRPLPASIDALALVAA
                     TYAENRPGLSWRGIQDTTNDPIVVGRGARAYLNYQMSEDYYRGGQMIWLEADALIRAG
                     SNGKRSLDDFARAFFGVNDGEWKVQDLYTFEDVVATLNGVHPHDWSTFLRDRLDGRTG
                     LTGGIEAGGWKLVYKDEPNAYAKGAGSRGGADFTYSIGLTIGKDGSIGDVRWDGPAFN
                     AGIGSGTQVIAVNGTAYDGDVLEEAIKAAKTGKAPIELLVKEFDRYRTVKIDYHGGLR
                     HPHLERIKDKPDYLTPILSARK"
     gene            1195..2016
                     /locus_tag="PK818_10715"
     CDS             1195..2016
                     /locus_tag="PK818_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HQY83240.1"
                     /translation="MRFFHLAITMCGISLLAGCAGPQPRPGAPAASARLTPPAIAATP
                     VAAARQPLLQTLARLAPDADRGVLALALQARECALRNGDADADARLAVIDYSLPSTHK
                     RLWVFDLAEARLLFHEHVAHGQGSGENVARRFSNVEGSYQTSLGLYRTAETYQGGNGY
                     SLRMDGLDPGFNDAARRRAIVMHGAWYADPDLIRARGRLGRSQGCPALREGIARVVID
                     AMKQRQLLFAYADDAAWLQRGRSFACDGRDARTILAAARGDAAQGGGAIGAISAP"
     gene            complement(2077..>2367)
                     /locus_tag="PK818_10720"
     CDS             complement(2077..>2367)
                     /locus_tag="PK818_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HQY83241.1"
                     /translation="VMAKLRGLRGTPLDVFGYSAERRGERRLIADYEKTIAGLFDRLD
                     AGNVDLAAAIASIPEHIRGYGHVKEAHLHKAKAREADLLGEWVNPLRVVQAA"
BASE COUNT          377 a          833 c          810 g          347 t
ORIGIN      
        1 cagatgggcg gcaacggcct ggagcaccag cgcagcagcg agaacggcgt cggcaccgac
       61 tacttcaccg gctggaagga gaagcagggc ttcaccgacc tgctggcgca cgagttcgtg
      121 cattcgtgga acggcaagta ccggcgcggc aaggacctgt gggtgccgaa cctcaacacg
      181 ccgatgcagg acagcctgct gtgggtgtac gaaggccaga cccagtactg gggcaacgtg
      241 ctggccgcgc gcagcggcat gcgcccgctg ccggcctcga tcgacgcgct ggcgctggtg
      301 gccgccacct atgccgagaa ccgcccgggc ctgtcctggc gcggcatcca ggacaccacc
      361 aacgacccga tcgtggtcgg ccgcggcgcg cgcgcctacc tcaactacca gatgagcgag
      421 gactactacc gcggcggcca gatgatctgg ctggaagccg acgcgctgat ccgcgccggc
      481 agcaacggca agcgctcgct ggatgatttc gcacgggcgt tcttcggcgt caacgacggc
      541 gaatggaagg tgcaggacct ctacaccttc gaggacgtgg tggccacgct caacggcgtg
      601 catccgcacg actggtcgac gttcctgcgc gaccgcctgg acggcaggac cggcctcacc
      661 ggcggcatcg aggccggcgg ctggaagctg gtctacaagg acgagcccaa tgcctatgcc
      721 aagggcgcgg gcagccgcgg cggcgccgac ttcacctatt cgatcgggct taccatcggc
      781 aaggacggca gcatcggcga cgtgcgctgg gacggccccg cgttcaatgc cggcatcggc
      841 agcggcacgc aggtgatcgc ggtcaacggc accgcctacg acggcgacgt gctggaggag
      901 gcgatcaagg cggccaagac cggcaaggcg ccaatcgagc tgctggtcaa ggagttcgac
      961 cgctaccgca cggtgaagat cgactaccac ggcggcctgc gccatccgca cctggagcgg
     1021 atcaaggaca agccggatta cctcacgccg atcctgtccg cacgcaagtg atgcgcgctg
     1081 cgaacgcggc ggttggatga tcgcgttcgc agtttcgcgt taaggattcg ttgccgttca
     1141 gccccgcgca acccattcag cgacatgcgc cggcgatgcc gttaaggtcc cgccatgcgc
     1201 tttttccacc ttgccatcac catgtgcggc atcagcctgc tcgccggctg cgcgggtccg
     1261 caacccaggc ccggcgcccc cgcggcgtcg gccagactga cgccgccggc catcgccgcc
     1321 accccggttg cggctgcccg gcaaccgctg ctgcagaccc tcgcccggct cgcgccggat
     1381 gccgaccgcg gcgtgctggc gcttgcgttg caggcgcgcg agtgcgcgct gcgcaacggc
     1441 gatgcggacg ccgatgcgcg gctggcggtg atcgactatt cgctgccatc cacgcacaag
     1501 cggctgtggg tgttcgatct tgccgaggcc aggctgctgt tccatgagca cgtcgcccac
     1561 ggccagggca gcggcgaaaa cgtcgcacgg aggttttcga acgtcgaggg cagctaccag
     1621 acaagccttg gcctgtaccg caccgcggaa acctaccagg gcggcaacgg ctactcgctg
     1681 cgcatggatg gcctcgaccc cggattcaac gatgccgcgc gccggcgtgc gatcgtcatg
     1741 cacggtgcct ggtatgccga tccggacctg atccgcgccc gtggccgcct cggccgcagc
     1801 cagggctgcc cggcgctgcg cgagggcatc gcccgggtcg tcatcgacgc catgaagcag
     1861 cggcaattgc tgttcgccta cgccgacgat gcggcgtggc tgcaacgcgg ccgcagcttc
     1921 gcctgcgacg gccgcgatgc gcgcacgatc ctcgccgccg cgcgcggcga tgcggcccag
     1981 ggtggcggcg cgatcggcgc gatctccgcg ccgtgatcgc gggtcggcgc ttcgcctgcg
     2041 tcgcaggcaa accgcggcag ccgcagttcg cggatatcaa gccgcctgca ccacccgcag
     2101 cgggttgacc cattccccca gcaggtccgc ctcgcgcgcc ttggccttgt gcaggtgcgc
     2161 ttccttgacg tgcccgtagc cgcggatgtg ctccgggatg ctggcgatgg cggcggccag
     2221 gtccacgttg ccggcatcca gcctgtcgaa caggccggcg atggtcttct cgtagtcggc
     2281 gatcagccgg cgctcgccgc ggcgttcggc gctgtagccg aacacgtcca gcggggtgcc
     2341 gcgcaggccg cgcagcttcg ccatcac
//
LOCUS       DAORUK010000234         2347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_69102_length_2347_cov_34.013525, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000234 DAORUK010000000
VERSION     DAORUK010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69102_length_2347_cov_34.013525"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PK818_10725"
     CDS             <1..627
                     /locus_tag="PK818_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83242.1"
                     /translation="ADKALAMLGALYADHGIEVIVVDDGDPEPFIEPRTNLDLKVLRL
                     PRKDAPKSPVTCWNEGVKVASGDVIALSCIEVLHRGRVLPAMEEALESVGDDGYILAA
                     AWCPEQRAWHTHSSLIVPQCPPGTGLAFLGMMYRSLFERAGGFDEAYRDGAGYEDRDF
                     IHRLHRAGAQFTIRDDLVVDHPKTGATIKWPAEAFSRNKMLFEEKWIN"
     gene            615..1259
                     /locus_tag="PK818_10730"
     CDS             615..1259
                     /locus_tag="PK818_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83243.1"
                     /translation="MDQLSVVVVQTNDYLGRGAEYVKKLRRQVRKHLTLPHEFFVVTD
                     DPASFYPRMKVKPSALPGWWEKLRLFKPGMFPDGRVLFLDLDTFIVGNIDDIASYDGP
                     FATLRDFWRKDGLGPAVMIWRTDTGLGIWEEFVAEGMKMTHPQGDQGFLENLDQGHFR
                     RRIDILQDKFPGRFVSYKTHCVTCIPDGASVVCFHGKPRPHEATGWAKEYWDGQ"
     gene            1198..1851
                     /locus_tag="PK818_10735"
     CDS             1198..1851
                     /locus_tag="PK818_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83244.1"
                     /translation="MASRARMRRPAGQRSIGMASNVQIANSALTKLGASRITALTDNV
                     KAAREINAIFELRRDYLLRTHNWSFAMTRANLPALSEAPAWGYTTLYQLPTDCLRVVQ
                     VNDTWVVPGLSDYTSGPDSEPYKITGRRIETDIGAPLKLRYIKRVTDPAQFDAAFVEV
                     FASDLADQVCEALTQSNTKREATRAVLRQSLLEAVRSNAIELPPEAIPDDSWILSRL"
     gene            1851..>2347
                     /locus_tag="PK818_10740"
     CDS             1851..>2347
                     /locus_tag="PK818_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83245.1"
                     /translation="MPKANPFLLSFSSGELSPLFDGRVDLDEYQSGCRVLENFVPLIE
                     GPAERRGGTRFVREVKDSTDRCVWLRFEFNTEQAYVLEIGDQYMRFYTDHGIVMNGTV
                     PLEVASPWVAADLVDADGNSKLRSVQSGDILYITHTDGDYQPQKLTRSGALSWAIADF
                     SPDGGP"
BASE COUNT          431 a          758 c          741 g          417 t
ORIGIN      
        1 gccgacaagg cgctggccat gctgggcgca ctctatgccg accatggaat tgaggtcatc
       61 gtcgtggatg acggcgaccc cgagccgttc atcgagccgc gcacgaacct ggacttgaag
      121 gtgctgcgcc tgccaaggaa ggatgcaccg aaatctccgg tcacttgctg gaacgagggc
      181 gtcaaggtgg ccagcgggga tgtgatcgcg ctgtcctgca ttgaagtgct gcatcgcggc
      241 agggtgctgc cggccatgga agaggcgctg gaatccgtcg gtgacgatgg ctacatcctg
      301 gccgccgcct ggtgcccgga acagcgcgca tggcataccc acagcagcct gatcgtgccg
      361 caatgcccgc ccggcaccgg cctggccttc ctcgggatga tgtaccgcag cctgttcgag
      421 agggcgggcg ggttcgacga agcctaccgc gacggcgccg gctacgaaga ccgcgacttc
      481 atccatcggc tgcaccgtgc cggggcgcaa ttcaccatcc gcgacgatct ggtggtcgat
      541 cacccgaaga ccggcgcgac gatcaagtgg ccggctgaag ccttctcgcg caacaagatg
      601 ctgttcgaag aaaaatggat caactgagcg tcgtcgtcgt gcagaccaac gattacttgg
      661 ggcgtggcgc cgagtacgtg aagaagctgc gccggcaagt gcgaaagcat ctgaccctgc
      721 cgcacgagtt tttcgtcgtg accgacgacc cggccagctt ctacccgcgc atgaaggtca
      781 agccgtctgc cctgcccggc tggtgggaga agctgcgcct gttcaagccc ggcatgttcc
      841 cggatgggcg cgtgctgttt ctcgatctgg ataccttcat cgtcggcaac atcgacgaca
      901 ttgccagcta cgatggcccg ttcgccacgc tacgtgactt ctggcgcaag gatggcctgg
      961 ggccggccgt catgatctgg cgcaccgata ctggcctcgg catctgggaa gagttcgtcg
     1021 ccgagggtat gaagatgacg cacccgcagg gcgaccaggg cttcctggag aaccttgacc
     1081 aggggcattt ccgccgccgc atcgacatcc tgcaggacaa gttcccgggg cggttcgtgt
     1141 cctacaagac gcattgcgtc acctgcattc cggacggtgc gagcgtcgtc tgtttccatg
     1201 gcaagccgcg cccgcatgag gcgaccggct gggcaaagga gtattgggat ggccagtaac
     1261 gtacagatcg ccaattccgc gctgaccaag ctcggagcgt cgcgcattac cgcgctgacc
     1321 gacaacgtga aggcagcgcg tgaaataaat gccatcttcg aactgcgccg cgactacctg
     1381 ctgcgcaccc acaactggtc gttcgccatg acgcgcgcca acctgcccgc gctcagcgag
     1441 gcgccggcat ggggatacac caccctctac cagttgccga ccgactgcct gcgcgtggtg
     1501 caggtcaacg acacatgggt cgtgccgggt ctgtccgatt acacctccgg gccggattcc
     1561 gagccgtaca agatcaccgg ccggcgcatc gagaccgaca tcggcgcccc gctcaagttg
     1621 cgctacatca agcgcgtgac cgatccggcg cagttcgatg cggccttcgt ggaagtcttc
     1681 gccagcgacc tggccgacca ggtgtgcgag gcgctgacgc aatcgaacac caagcgcgaa
     1741 gccacccgcg ccgtgctgcg acagtcgctg ctggaagccg tccggtcgaa cgccatcgag
     1801 ctgccgccgg aagccatccc cgacgattcc tggattctgt cgaggctgta atgcccaagg
     1861 ctaatccctt cctcctctcc ttcagttccg gcgaactgtc gccgctgttc gacgggcggg
     1921 tcgatctgga tgaataccag tccggatgcc gcgtcctgga gaactttgtc ccgctgatcg
     1981 aagggccggc ggagcgccgt ggcggcacgc gcttcgtgcg cgaggtgaag gattcgacag
     2041 accgctgcgt ttggctgcgc ttcgagttca ataccgagca agcctacgtg ctggaaatcg
     2101 gcgatcagta catgcgcttc tacaccgatc acggcatcgt catgaatggc accgtgccgc
     2161 tggaagtggc ctcgccctgg gtggcggctg atctggtgga tgcggacggc aattccaagc
     2221 tgcgctcggt gcagtccggc gacatcctct acatcaccca cacggatggc gactaccagc
     2281 cgcagaagct cacccgctcc ggcgcgctgt cctgggcgat tgccgacttc tctccggacg
     2341 gcgggcc
//
LOCUS       DAORUK010000235         2338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_69554_length_2338_cov_17.184406, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000235 DAORUK010000000
VERSION     DAORUK010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69554_length_2338_cov_17.184406"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PK818_10745"
     CDS             <1..204
                     /locus_tag="PK818_10745"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808006.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HQY83246.1"
                     /translation="HHGGGVGMGYSQHSGVVIACDGTPEADRRIARVLWNDPATGVMR
                     HADAGYEIARQCAKEQGLKLPML"
     gene            complement(242..1720)
                     /locus_tag="PK818_10750"
     CDS             complement(242..1720)
                     /locus_tag="PK818_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQY83247.1"
                     /translation="MDAMERHFAPFRANTVGNAQTFHSPYGEQRIVYADWTASGRLYR
                     PIETRLLESFGPFVGNTHSESSETGRAMTLAYHEAHRILKAHANAGPDDLIISTGTGM
                     TGVINKFQRILGLKAPQGLRRFLNLTEAERPVVFITHMEHHSNQTSWYETIADVVVVP
                     PDEQGLVDLAALESLLHDYRDRPLKIGAFTACSNVTGVSTPYHAMARLMHRAGGVAFV
                     DFAASAPYVDIDMHPADPEQRLDAVLWSPHKFLGGPGSSGALIFNRRLYRNTVPDDCG
                     GGTVNWTNPWGEYSFLDDIEAREDAGTPGFLQTIKAALAVRLKDQMGVAAMRQREHEI
                     VPYVMDALEAIPGVNVLARTRRDRLAIFSFHVTGIHYNLIVQLLNDRFGVQARGGCSC
                     AGTYGHYLLHVDPTLSHSITDRIDQGDLSDKPGWVRISFHPTTSTAEIDHTLDAVREI
                     VAHVHEWAREYEYSPVTNEFTLRGADGNAPMARVKRWFELDR"
     gene            1784..2002
                     /locus_tag="PK818_10755"
     CDS             1784..2002
                     /locus_tag="PK818_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase"
                     /protein_id="HQY83248.1"
                     /translation="MDIEALIRAALREAGYGPDAVGSALPRILRILQAEDVRIEVGRS
                     LSRKEREYVRLQLELGLNVSEIVAGLRS"
     gene            2078..>2338
                     /locus_tag="PK818_10760"
     CDS             2078..>2338
                     /locus_tag="PK818_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83249.1"
                     /translation="MAKKILFAPDTSDHPNWGCRFMGDWYRRELARLGCAATQRVGSR
                     WFFRPQQGVQAPATWADLQRIAAEVSAGRALEQVAHSLRDCDL"
BASE COUNT          365 a          732 c          856 g          385 t
ORIGIN      
        1 caccacggcg gcggcgtcgg catgggctat tcgcagcaca gcggcgtggt gatcgcctgc
       61 gacggcaccc cggaagcgga caggcggatc gcccgcgtgc tgtggaacga ccccgccacc
      121 ggggtgatgc ggcatgcgga tgccggttac gagatcgccc ggcagtgcgc gaaggaacag
      181 ggcctgaagc tgccgatgct gtgagcggcg ggcaccgcta gcgccccggg cccgcgcggg
      241 ctcagcgatc cagttcgaac cagcgcttga cgcgcgccat cggcgcgttg ccatccgcgc
      301 cgcgcagggt gaactcgttg gtgaccggcg aatattcgta ttcccgggcc cattcgtgca
      361 cgtgggcgac gatttcgcgc acggcatcga gggtgtggtc gatttccgcc gtcgaggtgg
      421 tcggatggaa ggaaatccgc acccagcccg gcttgtccga caggtcgccc tggtcgatgc
      481 gatcggtgat cgaatgcgac agggtcggat ccacgtgcag caggtagtgg ccgtaggtgc
      541 cggcgcagga acagccgccg cgggcctgca cgccgaagcg gtcgttgagc agttgcacga
      601 tcaggttgta gtggatcccg gtgacatgga acgagaagat cgcgagccgg tcgcggcgcg
      661 tgcgcgccag cacgttgaca cccgggatcg cctccagcgc atccatcacg tacgggacga
      721 tctcgtgttc gcgctggcgc atcgccgcga cgcccatctg gtccttcagc cgcaccgcaa
      781 gcgcggcctt gatggtctgc aggaatccgg gcgtgccggc gtcctcgcgg gcctcgatgt
      841 cgtcgaggaa ggagtactcg ccccacgggt tggtccagtt cacggtgccg ccgccgcaat
      901 catcgggaac ggtattgcgg tagagcctgc ggttgaagat cagcgcgccg gacgagcccg
      961 gcccgccgag gaacttgtgc ggcgaccaca gcacggcatc caggcgttgc tcggggtccg
     1021 ccgggtgcat gtcgatgtcc acgtatggcg ccgacgcggc gaagtcgacg aaggccacgc
     1081 cgcccgcgcg gtgcatcagc ctggccatcg cgtggtaggg cgtggacacg ccggtgacgt
     1141 tcgagcaggc ggtgaaggcg ccgatcttca gcgggcggtc gcgatagtcg tgcagcagcg
     1201 actccagcgc ggcgaggtcg accaggccct gctcatccgg cggcaccacc acgacgtcgg
     1261 cgatggtctc gtaccaggag gtctggttgg agtggtgctc catgtgggtg atgaacacca
     1321 ccgggcgttc cgcctcggtg aggttgagga agcggcgcag gccctgtggc gccttgagcc
     1381 cgaggatgcg ctggaacttg ttgatcaccc cggtcatgcc ggtgccggtg gagatgatca
     1441 ggtcatcggg tccggcgttc gcatgtgctt tcaggatgcg atgcgcctcg tggtaggcaa
     1501 gcgtcatcgc gcgcccggtt tcgctgcttt ccgagtgggt attgcccacg aacgggccga
     1561 agctttccag cagccgcgtc tcgatcggcc ggtacaggcg gccgctggcg gtccagtcgg
     1621 catagacgat tcgctgctcg ccatatggcg agtggaatgt ctgcgcgttg ccaacggtgt
     1681 tggcgcgaaa cggggcgaag tggcgttcca tggcatccat gcgggcattt tcgcctgttc
     1741 cgtgggccac aatacagccc ctgctgaccg acccggatgt gccatggaca ttgaagcgct
     1801 gatccgtgcc gccctgcgtg aagccggcta cggccccgac gcggtgggtt cggcgctgcc
     1861 caggatcctg cgcatcctgc aggcggagga cgtgcgcatc gaggtgggcc gcagcctgtc
     1921 gcgcaaggaa cgcgagtacg tgcgcctgca gctggagctc gggctcaacg tgtcggaaat
     1981 cgttgccggg ttgcggtcat gaccgggcgc tggcattcgg accgatggca ggacgcggga
     2041 aagccgggac aggtcccgcc ggcggaggcc tgacccgatg gcgaagaaga tcctgttcgc
     2101 acccgatacc tccgaccacc cgaactgggg atgccggttc atgggcgatt ggtaccggcg
     2161 cgaactggcg cggcttgggt gcgcggccac gcagcgggtc ggcagccgct ggttcttccg
     2221 gccacagcaa ggcgtgcagg cgccggccac ctgggcggac ctgcagcgga ttgccgcgga
     2281 ggtaagcgct ggccgcgcgc tggagcaggt ggcgcactcg ctgcgcgatt gcgacctg
//
LOCUS       DAORUK010000236         2314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_70844_length_2314_cov_19.593625, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000236 DAORUK010000000
VERSION     DAORUK010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70844_length_2314_cov_19.593625"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..401
                     /locus_tag="PK818_10765"
     CDS             78..401
                     /locus_tag="PK818_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014091282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HQY83250.1"
                     /translation="MNAAIPWLEWTGVHMSPWKLIGLVGALMFGGRWLVQFIASKRQG
                     KPVIPRLFWYMSILGSLMTLSYFIFGKNDAVGILQNLFPSFTACYSLYLDIRHRGWHR
                     DRAEH"
     gene            571..2280
                     /locus_tag="PK818_10770"
                     /pseudo
     CDS             571..2280
                     /locus_tag="PK818_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008575963.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
BASE COUNT          381 a          810 c          765 g          358 t
ORIGIN      
        1 gtggcctggc tgatcgtgcg cagccgccgc accgcggtcg aagagttgtc gaagtgatcg
       61 agtcgacgcc cgatgtcatg aacgccgcga ttccgtggct ggagtggacc ggcgtgcaca
      121 tgagcccgtg gaagctgatc gggctggtcg gcgcgctgat gttcggcggc cgctggctgg
      181 tgcagttcat cgccagcaag cgccagggca agccggtgat cccgcgcctg ttctggtaca
      241 tgagcatcct cggcagcctg atgacgctga gctatttcat cttcggcaag aacgacgcgg
      301 tcggcatcct gcagaacctg ttccccagct tcaccgcctg ctacagcctg tacctggaca
      361 tcaggcaccg cggctggcac cgcgaccgcg ccgagcatta gccgttcggc gcgatgcgga
      421 aaagggccag ggtggagttt tgcgacaagc ctgatcgaac ttgccagacg tggacggcgc
      481 aagatccacc ctggccccct ttccctcgat gccgggtgcc gccacgtgaa atccttcctc
      541 cttgcttgct gcctgctgct gcccacactt gtgcacgcac aagcaccagc gaacatcact
      601 gacaaggcct tcgagtcgat ctacaaggcc gagtgggact ggcgcagcca gcaggcgccc
      661 agctgggacg aggacagcga caaccgcgcg cgcaagccct cgaccacgct ggccgacgtc
      721 ggcccggcgg cacaggcgcg gcggctcgcc tacctcgatg gcgtcttgaa gcgactcgac
      781 ggcatcgatg ccggcaagct ctccgccgcc aaccgggtca acttcgcggt gtaccggccg
      841 cagatcgaac acatggcggc ggagatccgt taccgcgact acgagatgcc gttcaacgcg
      901 gacagtgcgt tctggtccga cctcggcttc atggcccgcg ccaccccgcg cgatgccgcc
      961 ggctaccgcg cctacgccgc gcgcctgcgc gacgtgcccc gccatttcga gcagcaggtg
     1021 gccaacatgc gcgcgggcct ggcgcgcggg ttctcggtgc cgcgcgcggt gctggacggg
     1081 cgcgacgggt cgatcgcgat ggtcgccggg ctcaaggaca tcgagtcgtc caacttcttc
     1141 gatccgtatc gcagcatgcc ggcgtcgatc ccggccgccg aacaggaagc gttgcgcgcc
     1201 gaaggccggg ccgcgatccg cgatgcggtg gtccccgcgt tcgcgaagct gctggtgttc
     1261 ttccgcggcg aatacatccc gaacgcacgc accacgctgg ccgccgaggc gatgccggat
     1321 ggcgaggcct ggtatcgcga gcagatccgc agatacacca cgctggacct gtccgcggac
     1381 gagatccacg cgatcggcct ggccgaagtc gccgcgatcc gcgccgggat ggacaccatc
     1441 atcgacgggc tcggcttcca gggtgcatcc agggaaaccc gcttcgccga cttcctcgcc
     1501 ttcctgcgca cggacccccg gttctacgcc aagaccccgc aggaactgct ggaccgcgcc
     1561 gcgtggattt ccaagcgggt ggatgggcag atcggcaagc tggtcggcac cctgccgcgc
     1621 gggcgcttca ccatcgtgcc ggtgccgccg gacatcgcgc cgtactggac cgccggccgc
     1681 ggcggcgccg acacctattg ggtcaacacc tacaacctgc ccagccgccc gctgtacaac
     1741 ctgcccgccc tgaccctgca tgaatcggcg ccgggtcatt cgctgcaggg ctcgctggcg
     1801 cgcgagcagg gcgacgtgcc cgatttccgc aaggagtaca tcagcgccta tggcgagggc
     1861 tggggcctgt attcggaatg gctgggcaag gagatgggca tctacgaaac cccgtacgag
     1921 gatttcgggc gcctgaccta cgcgatgtgg cgcgcctgcc gtctggtgat cgacaccggc
     1981 gtccaccaca agggctggac ccgcgagcag gcgctggcct tcctgcgcga caacaccgca
     2041 ttgagcgagc acgaggtgac caccgaggtc gaccgctaca tcagctggcc cggccaggcc
     2101 ctgagctaca agctgggcga gatcacgatc atgcgcctgc gccgcgaagc cgaagccgcg
     2161 ctgggcccga agttcgacgt ccgcgccttc cacgacgccg tgctggcgca gggctcggtg
     2221 ccgctgccgg tgctggactc gcagatacgg gcgttcatcg ccgaggccaa gggccgctag
     2281 ccggacgccc cgcgctggtg ggcggccgcc cata
//
LOCUS       DAORUK010000237         2297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_71750_length_2297_cov_17.249331, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000237 DAORUK010000000
VERSION     DAORUK010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71750_length_2297_cov_17.249331"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..407)
                     /locus_tag="PK818_10775"
     CDS             complement(33..407)
                     /locus_tag="PK818_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HQY83251.1"
                     /translation="MQFNLERPDYTYFLRGADGTGALVNDRRLGDSFVIAPDTLVEAW
                     PVRDALAMTPADLDPLLALAPELVVLGTGAAQRFPPAAVMAACLQRGIGIEVMANAAA
                     ARTYSVLAGEGRRVVAGFILAA"
     gene            complement(425..730)
                     /gene="yhbY"
                     /locus_tag="PK818_10780"
     CDS             complement(425..730)
                     /gene="yhbY"
                     /locus_tag="PK818_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004087669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HQY83252.1"
                     /translation="MPIALTNAQIRFLRGQAHDLKAILQVGGKGISDALAVEVDGALE
                     HHELIKVKVAAADREARDAMIAALAERTDAALVQRIGHTAVLYRPSKDKRHIVLPRA"
     gene            800..1462
                     /gene="rlmE"
                     /locus_tag="PK818_10785"
     CDS             800..1462
                     /gene="rlmE"
                     /locus_tag="PK818_10785"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HQY83253.1"
                     /translation="MATRSKSSQRWLKEHFSDPYVKKAQAEGMRSRAAYKLEELVERD
                     QLLKPGMVVVDLGAAPGGWSQWVRQELDQMERGLPDGARRGRVIASDILEMPSLAGVE
                     FLHGDFRDDAVLSRLLESLDGGQVDLVLSDMAPNKSGMDAVDQPRAMHLAELAMDFAD
                     GRLRPGGTFLIKLFQGTGFDEYVRELRRRYAKVAIRKPAASRKRSPEVYALAQGKLAQ
                     MK"
     gene            1472..>2297
                     /locus_tag="PK818_10790"
     CDS             1472..>2297
                     /locus_tag="PK818_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HQY83254.1"
                     /translation="MNDLAKNLLLWVVVAVVLMVVFQSFSPKLAASQEVVYSQFVQEV
                     QEDRIKKVDIASNERTVKFERGDGSTGTTTAPVRDQELISDLIAHKVEIKQAPPDTGV
                     SFWSLVLNFLPVLLIIGFWLFMMRQMQQGGGKGAMSFGRSRAKLLNEDQTKVTFGDVA
                     GCDEAKEEVSELVEFLRDPGRFQKLGGKIPRGVLMVGPPGTGKTLLAKAIAGEAKVPF
                     FSISGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGSGMGGGHDE"
BASE COUNT          367 a          754 c          824 g          352 t
ORIGIN      
        1 cccggcggcc acgcggagcc tggcgccagc ggctatgccg cgaggatgaa ccccgccacc
       61 acccgccgtc cttcccccgc cagcaccgaa taggtgcgcg ccgcggccgc gttggccatc
      121 acctcgatgc cgatcccgcg ctgcaggcag gcggccatca ccgccgcggg cgggaagcgc
      181 tgcgccgcgc cggtgccgag caccaccagc tccggcgcca gcgcgagcag cgggtcgagg
      241 tccgccgggg tcatcgccag cgcatcgcgc accggccagg cttcgaccag cgtgtccggg
      301 gcgatcacga aactgtcgcc gaggcggcgg tcgttgacca gcgcgccggt gccgtcggca
      361 ccacgcagga agtaggtgta gtcggggcgt tcgaggttga actgcatcgc gccgggcgct
      421 ggggtcaggc ccgcggcagc acgatgtgcc gcttgtcctt gctgggccgg tacagcaccg
      481 ccgtatggcc gatgcgctgc accagcgcgg cgtcggtgcg ttcggcgagg gcggcgatca
      541 tggcgtcgcg cgcctcgcgg tcggccgcgg ccaccttcac cttgatcaac tcgtggtgtt
      601 ccagggcgcc gtcgacctcg accgccaacg cgtcgctgat gcccttgccg ccgacctgca
      661 ggatcgcctt gaggtcatgt gcctggccac gcaggaagcg gatctgggcg ttggtcaggg
      721 cgatgggcat gcgctgcggg aaggacggga acaggcggca gggtatcatg agaccccatt
      781 ccacctggcg ttggcggccg tggctacccg cagcaaatcc agccagcgct ggctcaagga
      841 acacttctcc gacccctacg tgaagaaggc gcaggcggag gggatgcgat cgcgcgcggc
      901 ctacaagctg gaggagctgg tggagcgcga ccagctgctg aagccgggca tggtggtggt
      961 cgacctgggc gcggccccgg gcggctggtc gcaatgggtg cggcaggagc tggaccagat
     1021 ggaacgcggc ctgccggacg gcgcccggcg cgggcgggtg atcgccagcg acatcctgga
     1081 gatgccgtcg ctggccgggg tcgagttcct tcacggcgac ttcagggacg atgccgtcct
     1141 atcccgcttg ctggaatcgc tggacggggg ccaggtcgac cttgtcttgt ccgacatggc
     1201 ccccaataag agcggtatgg atgcggtcga ccagccgcgc gcgatgcacc tggcggaact
     1261 cgccatggac ttcgccgacg gccggctccg ccccggtggc accttcctga tcaagctgtt
     1321 ccagggaacc ggattcgacg aatacgtgcg ggagctgcgg cggcgttacg ccaaggtggc
     1381 catccgcaag ccggcggctt cgcgcaagcg ctcgccggag gtgtacgccc tggcacaggg
     1441 caaactcgcg caaatgaagt aaaaggcgga catgaacgat cttgcgaaga acttgctgct
     1501 ctgggtggtc gtcgccgtcg tgctgatggt ggtgttccag agcttctcgc cgaagctggc
     1561 cgcgagccag gaagtggtct attcgcagtt cgtgcaggag gtgcaggagg accgcatcaa
     1621 gaaggtcgac atcgccagca acgagcgcac ggtcaagttc gagcgcggcg acggttcgac
     1681 cggcaccacc accgcgccgg tgcgcgacca ggagctgatc agcgacctga tcgcccacaa
     1741 ggtcgagatc aagcaggcgc cgccggatac cggggtgtcg ttctggtcgc tggtgctgaa
     1801 tttcctgccg gtgctgctga tcatcggctt ctggctgttc atgatgcggc agatgcagca
     1861 gggtggcggc aagggcgcga tgtcgttcgg ccgctcgcgc gccaagctgc tcaacgagga
     1921 ccagaccaag gtcaccttcg gcgacgtcgc cggctgcgac gaggccaagg aggaagtctc
     1981 cgagctggtc gagttcctgc gcgacccggg ccgcttccag aagctcggcg gcaagatccc
     2041 gcgcggcgtg ctgatggtcg gcccgcccgg caccggcaag accctgctgg ccaaggcgat
     2101 cgccggcgag gccaaggtgc cgttcttctc gatctccggt tccgacttcg tcgagatgtt
     2161 cgtcggcgtc ggcgccagcc gcgtgcgcga catgttcgag caggccaaga agcacgcgcc
     2221 gtgcatcatc ttcatcgacg agatcgacgc ggtcggccgc catcgcggtt ccggcatggg
     2281 cggcggccac gacgagc
//
LOCUS       DAORUK010000238         2283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_72526_length_2283_cov_16.958707, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000238 DAORUK010000000
VERSION     DAORUK010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72526_length_2283_cov_16.958707"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PK818_10795"
     CDS             complement(<1..646)
                     /locus_tag="PK818_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83255.1"
                     /translation="MQTLTTRARRAPLAMALFVAMVAPGLAFAQTAKERELEARIAQL
                     EAQVQALIGAQQQQQATLAQTQTTLDQVRVAQAAPADGKPKIQSSPILSAGNPDGRFS
                     YGGFIKVDAMVTDTSDGEIADGSAGRLFYLPGAIPVGGVDESSADIDTHAQFSRFWFA
                     ADGEVQGHKTRAYLEFDLFGGGSSNLGNQASTNTHALTLRQAYVSWDKWLAGQTW"
     gene            882..>2283
                     /gene="acs"
                     /locus_tag="PK818_10800"
     CDS             882..>2283
                     /gene="acs"
                     /locus_tag="PK818_10800"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HQY83256.1"
                     /translation="MRHRDYVREYQAAIDEPDAFWSRIAGRLDWMRAPTRIKDVSFDA
                     ADFRIRWFEDGELNASVNCLDRHLAEHGDQVAIIFEPDDPSAPSQKLTYRELHARTCK
                     LANALRNLGVGKGDRVTIYLPMIPEAAVAMLACARIGAVHSVVFGGFAAQSIADRVQD
                     CQTKLIITADEGMRGGKRVPLKANVDDALRIAGTDSVETVLVVRHTHGAVDMQMPRDR
                     WYDAVVDGQPDTCEPERMNAEDPLFILYTSGSTGKPKGVLHTTGGYLVWAAYTHEIVF
                     DLKPDDVFWCSADVGWITGHSYIVYGPLANRATSVMFEGVPSWPNASRFWEVIDKHQV
                     TIFYTAPTAIRALMREGDHAVKRTSRKSLRLLGSVGEPINPEAWRWYHEVVGDERCPI
                     VDTWWQTETGGVLISPLPGATDLKPGSATLPLPGVRPALVDAAGQFLEGEASGNLVLL
                     DSWPGQMRTVYGDHQRF"
BASE COUNT          351 a          798 c          781 g          353 t
ORIGIN      
        1 accaggtctg gccggccagc cacttgtccc agctcacgta ggcctggcgc agggtcagcg
       61 cgtgggtgtt ggtcgacgcc tggttgccca ggttgctgct gccgccgccg aacaggtcga
      121 actccaggta ggcgcgggtc ttgtggccct gcacctcgcc atcggcggcg aaccagaagc
      181 gcgagaactg cgcgtgggtg tcgatgtcgg cgctggattc gtccacgcca ccgaccggga
      241 tcgcgcccgg caggtagaac aggcggccgg cgctgccgtc ggcgatctcg ccgtcgctgg
      301 tgtcggtcac catcgcgtcg accttgatga agccgccgta gctgaagcgt ccgtcgggat
      361 tgcctgcgga caggatcggc gaagactgga tcttcggctt gccatcggcc ggcgcagcct
      421 gcgcaacgcg cacctggtcc agcgtggtct gcgtctgcgc gagcgtcgcc tgctgctgtt
      481 gctgcgcgcc gatcagcgcc tgcacctgcg cttccagctg cgcgatgcgc gcttcgagtt
      541 cgcgctcctt cgcggtctgc gcgaacgcca gtcccggtgc caccatggcg acgaacaggg
      601 ccatcgccag gggcgcacgg cgggcgcggg tcgtgagtgt ctgcatggtt ccccctccca
      661 agggatgcac ggctgcatgc cgcgccagca gaccttgcgc cggcacgcca agggcgtcga
      721 ttcgcctttg gtcgaagacg ccgcgtggct gcgaccaatg tctaacaccg gcggcgcgcg
      781 cttgcggcac actcgtccca tcagtcgtcc tcgggaggga tgccatgagc aaccagcaag
      841 agcgttacca cgtccccgct gcattcgccg cgcattcggc catgcgccac cgcgactacg
      901 tccgcgagta ccaggccgcg atcgacgagc ccgacgcgtt ctggtcgcgc atcgccgggc
      961 gcctcgactg gatgcgcgcg ccgaccagga tcaaggacgt cagcttcgac gccgccgact
     1021 tccgcatccg ctggttcgag gatggcgaac tcaacgccag cgtcaattgc ctggaccgcc
     1081 acctggccga gcacggcgac caggtcgcga tcatcttcga gccggacgat ccctcggcgc
     1141 caagccagaa gctcacctat cgcgagctgc acgcgcgtac ctgcaagctg gccaacgcgc
     1201 tgcgcaacct cggcgtgggc aagggcgacc gggtcaccat ctacctgccg atgatccccg
     1261 aggccgcggt ggcgatgctt gcctgcgcgc ggatcggcgc ggtgcactcg gtggtgttcg
     1321 gcggcttcgc cgcgcagtcg atcgccgacc gcgtgcagga ctgccagacc aagctgatca
     1381 tcaccgccga tgagggcatg cgcggcggca agcgggtgcc gctgaaggcc aacgtcgacg
     1441 acgcgctccg gatcgccggg accgactcgg tcgagaccgt gctggtggtg cgccacacgc
     1501 atggcgcggt ggacatgcag atgccgcgcg accgctggta cgacgcggtg gtcgacggcc
     1561 agccggacac ctgcgaaccg gagcgcatga acgcggagga cccgctgttc atcctctaca
     1621 cctccggcag caccggcaag ccgaagggcg tgctgcacac caccggcggc tacctggtgt
     1681 gggccgccta cacccacgag atcgtgttcg acctcaagcc ggacgatgtc ttctggtgct
     1741 ccgccgacgt cggctggatc accggccaca gctacatcgt gtacggcccg ctggccaacc
     1801 gcgcgaccag cgtgatgttc gagggcgtgc cgagctggcc gaacgcctcg cgcttctggg
     1861 aggtgatcga caagcaccag gtcaccatct tctacaccgc gccgaccgcg atccgcgcgc
     1921 tgatgcgcga aggcgaccac gcggtgaagc gcacctcgcg caagtcgctg cgcttgctgg
     1981 gcagcgtcgg cgaaccgatc aacccggaag cctggcgctg gtaccacgag gtggtcggcg
     2041 atgagcgttg cccgatcgtg gacacctggt ggcagaccga gaccggcggc gtgctgatct
     2101 cgccgctgcc gggcgcaacc gacctcaagc cggggtcggc caccctgccg ctgccgggcg
     2161 tgcgtccggc gctggtggat gcggccggcc agttcctgga aggcgaggcc tccggcaacc
     2221 tggtgctgct ggattcgtgg ccggggcaga tgcgcacggt gtacggcgac caccagcgct
     2281 tca
//
LOCUS       DAORUK010000239         2281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_72624_length_2281_cov_18.482031, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000239 DAORUK010000000
VERSION     DAORUK010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72624_length_2281_cov_18.482031"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(292..1185)
                     /locus_tag="PK818_10805"
     CDS             complement(292..1185)
                     /locus_tag="PK818_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HQY83257.1"
                     /translation="MTAPTLYLVDASLYVFRAWHSMPDEFHDADGWPTNAVHGFARFL
                     LELLERERPRHIAVAFDEALDSCFRNALYPAYKANRDPAPPELKRQFVWCKALCKAVG
                     LTTLAHSDYEADDLIGSAAHRARGNGFRSVIVSADKDMSQLLHAHDEQYDFAKGVRWG
                     ADGVKARQGVHAHQIADYLALTGDAVDNIPGVSGIGAKSAAVLLAHFGTLDALLARID
                     EVPFLRLRGAAGLATRLREQRDHARLWRQLTTIPLDVPLPEGDFARGRGDDEELTALA
                     DWLGFGPLTRKRLSEAAGLAL"
     gene            complement(1195..1776)
                     /locus_tag="PK818_10810"
     CDS             complement(1195..1776)
                     /locus_tag="PK818_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010367372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQY83258.1"
                     /translation="MSPKLTLGALDTRRSTPTRQLSTPGPDAAQLQRLLQTAVRVPDH
                     GKRVPFRFLRIAGAARAMLADAASVRLRERDPDASEAVIDKLRTRFVLPPLTIAVVAK
                     LGIDEAIPEAERFSSASCVCLLLLQAAQAQGFGAQWLTGWIAYDRPFLEGTLGLAEDE
                     QLVGTIAIGTPLVAAPERDRPDPATLVTDWQPR"
     gene            1854..>2281
                     /locus_tag="PK818_10815"
     CDS             1854..>2281
                     /locus_tag="PK818_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HQY83259.1"
                     /translation="MAVIVGVARETAAGERRVALTPETCKKLVAAGATVRIQPGIGAG
                     AHFPDQAYVDAGAQLSDAATTDADIVPCVQPPAADAIAALKQGAVLVGSLQPQADAAR
                     GEAIVARGIVAFPLEKLPRTTRAQAMDVLSSQAGMAGYKAV"
BASE COUNT          346 a          785 c          822 g          328 t
ORIGIN      
        1 gtcaccgcga tcacgatcac cgccatgccc tggccgtggg tgcgctcgca ggattcccag
       61 tggtcgatct tcaccagccg cagccagtcg ccttcgtaca gggtgtgctt gaggttgtgg
      121 cccatgcgcc gatgctagcg caggtggatg gcgttgagcc tgctctgctc cctcccttgc
      181 gtagcagggg agggttgggg aggggtgctc tttgcttcga tgccggcaaa agcaaccccc
      241 tcccggcctc cccctgctta cgcaagggga ggagacaagc atgggaaagc cttacagcgc
      301 gagtcccgcc gcttcgctca agcgcttccg cgtcagtggg ccaaagccca accagtcggc
      361 gagggcagtg agttcctcgt catcgccacg gccacgggcg aaatcgccct ccggcaacgg
      421 cacgtcgagc ggaatcgtgg tgagttgccg ccacagccgg gcgtgatcgc gttgctcgcg
      481 caggcgcgtg gccaagcccg cggcaccgcg caggcgcagg aacggcactt catcgatgcg
      541 tgccagcagc gcgtccaggg tgccgaaatg cgccagcagc accgccgccg acttcgcgcc
      601 gatcccgctg acgccgggga tgttgtccac cgcatcgccg gtcaatgcca ggtaatcggc
      661 gatctggtgc gcgtgcacgc cctgccgcgc cttcaccccg tccgcacccc agcgcacgcc
      721 cttggcgaag tcgtactgct cgtcgtgcgc gtgcagcaac tgcgacatgt ccttgtcggc
      781 ggacacgatc acgctgcgga aaccgttgcc gcgggcacga tgcgcggcgc tgccgatcag
      841 gtcgtcggct tcgtaatcgg aatgggcgag cgtggtgagc cccaccgcct tgcacagcgc
      901 cttgcaccag acgaattggc gcttcaattc aggtggcgcg ggatcgcggt tggccttgta
      961 ggccggatac agcgcgttgc ggaagcagga atccagcgcc tcgtcgaagg cgacggcgat
     1021 gtgccgcggc cgctcccgtt ccagcagctc cagcaggaag cgggcgaagc cgtgcaccgc
     1081 gttggtcggc cagccatcgg catcgtggaa ctcgtccggc atcgaatgcc aggcgcggaa
     1141 cacgtacagc gaggcgtcga ccaggtacag cgtgggcgcg gtcatgcccg gtacctaccg
     1201 tggctgccag tcggtgacca gcgtggccgg atcgggccgg tcgcgctccg gcgccgcgac
     1261 cagcggggtg ccgatggcga tggtgccgac cagttgctcg tcctccgcca ggcccagggt
     1321 gccttccagg aacgggcggt cgtaggcgat ccagccggtc agccactgcg cgccgaagcc
     1381 ctgcgcctgc gccgcctgca gcagcaacag gcagacgcag gacgcgctgg agaagcgctc
     1441 ggcctccggg atcgcctcgt cgatgcccag cttcgccacc accgcgatgg tcagcggcgg
     1501 cagcacgaaa cgcgtgcgca gcttgtcgat caccgcctcg ctggcatccg ggtcgcgctc
     1561 gcgcaggcgg accgaggcgg cgtccgccag catcgcgcgc gcggcgccgg cgatccgcag
     1621 gaaccggaac ggcacccgct tgccgtggtc cggcacccgc accgcggtct gcagcagccg
     1681 ctgcagctgg gcggcgtccg ggcccggggt ggacagctgc cgggtcgggg tcgagcgccg
     1741 ggtgtcgagg gcgccaaggg tcagcttcgg ggacatcgtg gtggcggccg ccttgcaggg
     1801 agccgccatg gtagcggtag gatgcgttca tcgaatcgga gcaggagcag gcgatggcgg
     1861 tgatcgtagg cgtggcgcgc gaaacggcgg cgggtgagcg ccgggtcgcc ctgaccccgg
     1921 aaacatgcaa gaagctggtc gccgcggggg ccacggtgcg catccagccg ggcatcggtg
     1981 ccggcgcgca tttcccggac caggcctatg tcgacgccgg cgcgcagctg agcgacgccg
     2041 ccaccacgga cgccgacatc gtgccctgcg tgcagccgcc ggcggccgat gcgatcgccg
     2101 cgctgaagca gggcgcggtg ctggtcggca gcctgcagcc gcaggccgat gccgcccgcg
     2161 gcgaggcgat cgtggcgcgc gggatcgtcg cgttcccgct ggagaagctg ccgcgcacca
     2221 cccgcgcaca ggcgatggac gtgctcagct cgcaggccgg catggccggc tacaaggcgg
     2281 t
//
LOCUS       DAORUK010000240         2259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_73805_length_2259_cov_20.500000, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000240 DAORUK010000000
VERSION     DAORUK010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73805_length_2259_cov_20.500000"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PK818_10820"
     CDS             complement(<1..277)
                     /locus_tag="PK818_10820"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008573596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HQY83260.1"
                     /translation="MADIYGHLPRGPAGVMKAAIWSLQGLRSAWLHESSFRLEVCLFV
                     VLGPLGWMLGANGVERALLIGSCLLVMAMELMNSSMEAVIERYGAEHH"
     gene            complement(450..1685)
                     /locus_tag="PK818_10825"
     CDS             complement(450..1685)
                     /locus_tag="PK818_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQY83261.1"
                     /translation="MNFLEILRTAIFALRGNWLRSALTSLGVIIGIAAVIVMVSVGQG
                     TQAEIDKMVSGLGSQRLDISPGGGRGPGGARMSSSSFFTLTEEDVDAIRTEVQNVQFV
                     AGSLRGNTQVVFAENNGSTSWQGVQPEYFDINSWELVQGDGFTAQDYGSAGKVVILGE
                     TVRRTLFGEDSGIGQTIRLGRVPFTVAGTLKPKGQGGWGQDQDDVVMVPMETARRRLM
                     GSMGLPPGTVMQIALTVTDPKDLDYVQGEVEALLRQRHKIQPGEEDDFSVRNISQIVA
                     TRTATTNLMSKLLGAVAGICLIIGGIGIMNIMLVSVTERIREIGLRMAVGAGPGDVQR
                     QFLAEAMLISLIGGVIGIAFGVVGALVFSKIGQLPVELNTKVVLLAATFSICTGLFFG
                     YYPARKASLLDPIEALRSQ"
     gene            complement(1682..>2259)
                     /locus_tag="PK818_10830"
     CDS             complement(1682..>2259)
                     /locus_tag="PK818_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQY83262.1"
                     /translation="STLMNLIGCLDTPTAGSYACDGVDVSTLDAEELAVLRRDKIGFV
                     FQGFNLLPRMSALENVAMPMGYSNVPRIERLQRASAALAAVGLHNRGGHLPSELSGGQ
                     QQRVAIARALINRPPLLLADEPTGALDSKTGEEILALFKRLRDEGHTVVLITHDAEVA
                     AHCDRVLVMKDGELHEGTLQEGLHPPQGGSA"
BASE COUNT          349 a          827 c          736 g          347 t
ORIGIN      
        1 cgtggtgttc ggcgccgtag cgctcgatca ccgcctccat cgaggagttc atcagctcca
       61 tcgccatcac cagcaggcag ctgccgatca gcagcgcgcg ctcgaccccg ttggcgccga
      121 gcatccagcc cagcgggccg agcaccacga acaggcagac ctcgaggcgg aagctggatt
      181 cgtgcagcca cgccgaacgc aggccctgca gcgaccagat cgcggctttc atcacccccg
      241 ccggaccgcg cgggaggtgg ccatagatgt cagccatgcg taggcactct ggattggacg
      301 ggcatggcgg aacacggttg cggcatcgcc ggcgattttg ccacatggcc cccgccaccg
      361 cccgtgccgc gcccgaacgg gcacggcctg gaggagcgga gcagggctgg cgcgggcgac
      421 gcccatcgcc gcatccggga ctgcccgcgc tactgcgagc gcagcgcctc gatcggatcc
      481 agcagcgacg ccttgcgcgc cgggtaatag ccgaagaaca gcccggtgca gatcgagaag
      541 gtggccgcca gcagcaccac cttggtgttc agctccaccg gcagctgccc gatcttgctg
      601 aagaccagcg ccccgaccac gccgaacgcg atcccgatca ccccgccgat cagcgagatc
      661 agcatggcct cggccaggaa ctgccgctgc acgtcgcccg ggccggcgcc gaccgccatg
      721 cgcaggccga tctcgcggat ccgctcggtc accgagacca gcatgatgtt catgatgccg
      781 atgccgccga tgatcaggca gatgccggcc accgcgccga gcagcttcga catcaggttg
      841 gtggtggcgg tgcgggtggc gacgatctgg ctgatgttgc ggacgctgaa gtcgtcttcc
      901 tcgccgggct ggatcttgtg gcgctggcgc agcagcgctt ccacctcgcc ctgcacgtag
      961 tcgaggtcct tggggtcggt cacggtcagc gcgatctgca tcaccgtgcc cggcggcagg
     1021 cccatgctgc ccatcagccg gcggcgggcg gtttccatcg gcaccatcac cacgtcgtcc
     1081 tggtcctggc cccagccacc ctgccccttc ggcttcaggg tgccggccac ggtgaacggc
     1141 acccgcccca ggcggatggt ctggccgatg ccggaatcct cgccgaacag ggtgcggcgc
     1201 acggtttcgc ccaggatcac caccttgccg gcgctgccgt aatcctgcgc ggtgaagccg
     1261 tcgccctgca ccagctccca gctgttgatg tcgaagtact ccggctgcac gccctgccag
     1321 ctggtcgagc cgttgttctc ggcgaacacc acctgggtgt tgccgcgcag cgaaccggcc
     1381 acgaactgca cgttctgcac ctcggtgcgg atcgcgtcga cgtcctcctc ggtgagggtg
     1441 aagaagctgc tcgaactcat ccgcgcgccg cccggcccgc ggccgccacc cggcgagatg
     1501 tccagccgct gcgagcccag ccccgacacc atcttgtcga tctcggcctg ggtgccctgg
     1561 cccaccgaca ccatcacgat caccgcggcg atgccgatga tcacccccag cgatgtcagc
     1621 gcgctgcgca gccagttgcc gcgcagggcg aagatcgcgg tgcgcaggat ctcgaggaaa
     1681 ttcatgcgct gcctccctgc ggcggatgca ggccttcctg cagggtgccc tcgtgcagct
     1741 cgccgtcctt catcaccagc acgcggtcgc aatgcgccgc cacctcggca tcgtgggtga
     1801 tcagcaccac cgtgtggccc tcgtcgcgca ggcgcttgaa cagcgccagg atctcttcgc
     1861 cggtcttgct gtccagcgca ccggtcggtt cgtccgccag caacagcggc gggcggttga
     1921 tcagggcgcg cgcgatcgcc acgcgctgct gctggccgcc cgacagctcg ctgggcaggt
     1981 ggccgccgcg gttgtgcagg cccaccgccg ccagcgccgc ggacgcacgc tgcaggcgct
     2041 cgatgcgcgg cacgttggag tagcccatcg gcatcgccac gttctccagc gcgctcatcc
     2101 gcggcaacag gttgaagccc tggaacacga agccgatctt gtcgcggcgc aggaccgcca
     2161 gctcctcggc gtccagcgtc gacacgtcga cgccatcgca ggcgtagctg cctgcggtgg
     2221 gggtgtcgag gcagccgatc aggttcatca gggtcgact
//
LOCUS       DAORUK010000241         2251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_74248_length_2251_cov_17.588798, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000241 DAORUK010000000
VERSION     DAORUK010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74248_length_2251_cov_17.588798"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PK818_10835"
     CDS             <1..548
                     /locus_tag="PK818_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQY83263.1"
                     /translation="VEAVLANRAGAFEQLVRDYQGLCWYVIQRMVRHPEDTRELCQEA
                     FLRVHRYLHQYRFDSPLKSWIGQVAYSVAKRHLERKRIALVDPVGDEDGLSLLDNIGD
                     GGDLQQEHVQADDERELHAAIERLPPLQRTLLTLYHLEELPIVEIASITGLAEGTIKS
                     HLFRSRKTLRDLLTPRIGVAA"
     gene            545..973
                     /locus_tag="PK818_10840"
     CDS             545..973
                     /locus_tag="PK818_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83264.1"
                     /translation="MNHQDRQHPIDEARWQAQEKARRGDADADPDDLRIARALRRAPP
                     VDLPFDFASQVASLARAQAAANCLLEQRLLRGLVFVFALSAAVVIAWQGRGWTAGLAA
                     VLPGGSDAVGWSAAAALCVLANWGFAALRGQFGRGPRTSA"
     gene            1009..2070
                     /locus_tag="PK818_10845"
     CDS             1009..2070
                     /locus_tag="PK818_10845"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQY83265.1"
                     /translation="MSTTHRDQALDALLHPFAEGLLRWPQAGNALFLRAREGAALHAL
                     GVRGMVATQPFKPEAERLQRVGVELLDEDALPATRFPLVLVLPPRQREEARALLAKAC
                     TAVAPGGMVIAAVANDEGAKSREADLKLLAGGLNVISKHHCRVSWTRPDATFDAALVA
                     QWSKADAPRAVRSAEVPGGAFQTRPGVFAWDRVDAASRMLAQVLPDDLHGRVADFGAG
                     WGYLSLQVLARCPKVASLDLYEADARSLALADENLADAHLPVHCHWHDVARGVDERFD
                     AIVCNPPFHALGRGDRPDIGRAFIAAAASALKPGGRLWLVANRHLPYEQALGEGFAQV
                     RTLAQDGGFKIVEGEKARA"
BASE COUNT          330 a          781 c          807 g          333 t
ORIGIN      
        1 tcgtcgaagc cgtgctcgcg aaccgcgcgg gcgcgttcga gcagctggtg cgcgactacc
       61 aggggctgtg ctggtacgtg atccagcgca tggtgcggca cccggaggac acccgcgagc
      121 tgtgccagga agcgttcctg cgggtgcacc gctacctgca ccagtaccgc ttcgacagcc
      181 cgctgaagtc gtggatcggg caggtggcgt actcggtcgc caagcgccac ctggaacgca
      241 agcggatcgc gctggtggat ccggtgggcg acgaggacgg cctgtccctg ctcgacaaca
      301 tcggcgacgg cggcgacctg caacaggaac atgtgcaggc cgacgacgag cgcgagctgc
      361 atgccgccat cgagcgcctg ccgccgctgc aacgcacgct gctgaccctg tatcacctgg
      421 aagaactgcc gatcgtcgag atcgcttcca tcaccgggct cgccgaaggc accatcaaga
      481 gccatctgtt ccgtagccgc aagacactgc gcgatctcct cacgccgcgc atcggagttg
      541 ccgcatgaac caccaggacc gccaacaccc gatcgacgaa gcccgctggc aggcgcagga
      601 aaaggcccgc cgcggggatg ccgatgccga tcccgatgac ctgcgcatcg cccgcgcgct
      661 gcggcgtgcg ccgccggtgg acctgccgtt cgacttcgcc agccaggtcg catcgctggc
      721 gcgtgcgcag gcggcggcga actgcctgct ggagcaacgc ctgctgcgtg gactggtgtt
      781 cgtgttcgcc ttgtcggcgg cggtggtgat cgcctggcaa ggccgcggct ggaccgccgg
      841 gctggccgcg gtgttgccgg gtggcagcga cgcggtcggc tggagcgccg ccgccgcgct
      901 gtgcgtgctg gccaactggg gtttcgctgc gctgcgcggg cagttcggcc gcgggccgcg
      961 caccagcgcc tgatcgctgc cggtggaggg cctcgctaca ctgcgcggat gagcaccacc
     1021 catcgcgacc aggccctcga cgccctcctg catccgttcg ccgaaggcct gctgcgctgg
     1081 ccgcaggccg gcaacgcgct gttcctgcgc gcccgcgaag gcgcggccct gcatgcgctg
     1141 ggcgtgcgcg gaatggtcgc cacccagccg ttcaagccgg aagccgaacg gctgcagcgg
     1201 gtcggcgtgg agctgctgga cgaagacgcg ctgccggcga ccagattccc gttggtactg
     1261 gtgttgccgc cgcgccagcg cgaggaagca cgcgcgctgc tggccaaggc ctgcaccgcg
     1321 gttgcgccgg gcgggatggt gattgccgcg gtggccaacg acgagggcgc gaagtcccgc
     1381 gaagccgacc tgaagctgct cgcgggtggc ctcaacgtca tcagcaagca ccattgccgg
     1441 gtgtcctgga cccggcccga cgccacgttc gatgccgcgc tcgtcgccca atggtcgaag
     1501 gccgatgccc cgcgcgcggt ccgcagcgcg gaggtgccgg gcggcgcgtt ccagacccgc
     1561 cccggcgtgt tcgcctggga tcgggtggat gccgcgtcga ggatgctggc gcaggtcctg
     1621 ccggacgacc tgcacggccg cgtcgccgat ttcggcgccg gctggggcta cctgtccctg
     1681 caggtgctgg cgcgttgccc gaaggtggcc tcactcgacc tgtacgaggc cgatgcgcgg
     1741 tcgctggcgc tggccgacga gaacctcgcc gacgcgcacc tgcccgtgca ttgccactgg
     1801 catgacgtcg cccgcggcgt cgacgaacgc ttcgatgcga tcgtctgcaa cccgccgttc
     1861 catgcgctgg gccgcggcga ccgcccggat atcggccgtg cgttcatcgc cgccgccgcc
     1921 agcgcactca agcccggcgg ccgcctgtgg ctggtcgcca accgccacct gccatacgaa
     1981 caggcgctgg gcgaaggttt cgcgcaggtg cgcacgcttg cgcaggacgg cggcttcaag
     2041 atcgtcgaag gtgaaaaggc gcgtgcctag cggacgccat caaggcgtct gctgcgcctt
     2101 tgaacgaccg gccatggatg gccgggccgt gggtcgccag gcggcttggc tgggccatgg
     2161 atggcatcga ggtgctgcaa ggagacttgg atgaagctgg tcaagcacct cgccaacctg
     2221 ggctacggca gccgcaagga cgtgcagtgg a
//
LOCUS       DAORUK010000242         2173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_78663_length_2173_cov_16.567517, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000242 DAORUK010000000
VERSION     DAORUK010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78663_length_2173_cov_16.567517"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..560
                     /locus_tag="PK818_10850"
     CDS             66..560
                     /locus_tag="PK818_10850"
                     /inference="COORDINATES: protein motif:HMM:NF021517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HQY83266.1"
                     /translation="MIEQLEARPPDAGFEFRVRADRSATAGGLLGFFGLLALTAGAVA
                     AWSWGQGNVFAPFFAVAELSALGACLVLVWRRLERVELIGVRRDGVTVAQPPVPGAVR
                     LDVNWVRVERWPGRSDADLARLVLTSHGRAVEIGGFLAAPERGALERQLRQALARVRG
                     AEPV"
     gene            586..1473
                     /gene="coxB"
                     /locus_tag="PK818_10855"
     CDS             586..1473
                     /gene="coxB"
                     /locus_tag="PK818_10855"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HQY83267.1"
                     /translation="MKQIAKWTGRAAIALALAAQAPLALAQTHAERWQLNMTPGVTET
                     ARTAYEMHMIMLWICVVIGVIVFGAMFVAMFRFRKSKGAKPDTGFVHSTRLEILWTVI
                     PIVILVVMAWPATRSLILMMDTKDAEMTVKVTGYQWKWRYEVVDYRGAAQPINFMSSL
                     AYESNKTRMLGSGLDPTSPNYLLEVDRELVLPTDTKIRFLLSAEDVIHAWWVPALGWK
                     QDAIPGFVNEAWTSIDQAGTYRGQCAELCGKDHGFMPIVVRAVPRAEFEQWLAANGGT
                     LPAGQQLADTSAAAVPAGR"
     gene            1503..>2173
                     /locus_tag="PK818_10860"
     CDS             1503..>2173
                     /locus_tag="PK818_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014035723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQY83268.1"
                     /translation="MATHAATEHHDDHDHKPKGFLNRWLYSTNHKDIGTLYLVFAFIM
                     FLVGGAMSGVIRLELFQPGLQFVHPEFFNQMTTMHALVMIFGGVMPAFVGLANWMIPL
                     QIGAPDMALPRMNNWSFWLLPFAFLLLLLPLIIYLVTGQGAGGPAGGWTLYPPLSLQG
                     GSNVATTIFAIHLMGISSIMGAINIIATILNMRAPGMDLLKMPLFTWTWLITAFLLIA
                     VMPVLA"
BASE COUNT          322 a          735 c          742 g          374 t
ORIGIN      
        1 tcggcttgcc cccgcaatgg cccgacaact atcatttccc ggttccgccg tgacccttcc
       61 tggccatgat cgagcagctt gaggcccgtc cgccggacgc cggcttcgag ttccgcgtgc
      121 gggccgaccg ttcggcgacg gcaggtgggc tgctgggctt cttcggcctg ctggccctca
      181 ccgctggcgc cgtggcggcc tggagctggg ggcagggcaa tgtgtttgcg ccgttctttg
      241 ccgttgccga gttgtccgcc ctgggcgcgt gcctcgtgct ggtgtggcgg cggctggagc
      301 gggtggagtt gatcggggtg cggcgggacg gcgtcacggt ggcgcagccg ccggtgccgg
      361 gggccgtccg gctcgacgtg aactgggtgc gggtggagcg ttggccgggg cgcagcgacg
      421 cggacttggc aagactggtg ctgacctcgc acggacgcgc ggtggagatc ggcggcttcc
      481 tggccgcgcc cgaacggggc gccctggagc ggcaattgcg gcaggcgctg gcgcgggtgc
      541 gcggcgcgga gccggtgtga cggaatcgga accaaggtga cggcaatgaa gcagatcgcg
      601 aagtggacgg gccgggcggc gatcgcgctc gcactggccg cgcaggcgcc gctggcgctg
      661 gcgcagaccc acgccgagcg ctggcagctc aacatgacgc cgggtgtcac cgagaccgcg
      721 cgcactgcct acgagatgca catgatcatg ctgtggatct gcgtggtcat cggcgtgatc
      781 gtgttcgggg cgatgttcgt cgccatgttc cgtttccgga aatccaaggg cgccaagccc
      841 gacaccggct tcgtccacag cacgcgcctc gagatcctgt ggaccgtgat cccgatcgtc
      901 atcctggtgg tgatggcctg gccggccacg cgcagcctga tcctgatgat ggacaccaag
      961 gatgccgaga tgaccgtcaa ggtcaccggc taccagtgga agtggcgcta cgaggtggtg
     1021 gactaccgcg gcgcggcgca gccgatcaac ttcatgtcct cgctggccta cgagagcaac
     1081 aagacccgca tgctcggctc gggcctcgat cccacctcgc ccaactacct gctcgaggtc
     1141 gatcgcgagc tggtcctgcc caccgacacc aagatccgct tcctgctcag cgccgaggac
     1201 gtgatccacg cctggtgggt gccggccctg ggctggaagc aggacgcgat cccgggcttc
     1261 gtcaacgagg cctggacctc gatcgaccag gccggcacct atcgcggcca gtgcgccgag
     1321 ctgtgcggca aggaccacgg cttcatgccg atcgtggtgc gcgcggtgcc gcgtgccgag
     1381 ttcgaacaat ggttggccgc caatggcggc accctgccgg cgggccagca gctcgccgac
     1441 accagcgccg ccgccgtccc ggccggccgc tgattccccg aatgcgcaga ggctagacga
     1501 ccatggcgac gcacgcggcc accgaacacc acgacgacca cgaccacaag cccaagggct
     1561 tcctgaatcg ctggttgtac tcgaccaacc acaaggacat cgggaccctg tacctggtgt
     1621 tcgccttcat catgttcctg gtcggcggcg cgatgagcgg cgtgatccgc ctcgaactgt
     1681 tccagcccgg gctgcagttc gtgcaccccg agttcttcaa ccagatgacc accatgcacg
     1741 cgctggtgat gatcttcggc ggcgtgatgc cggccttcgt cggcctcgcc aactggatga
     1801 tcccgctgca gatcggcgcg cccgacatgg cgctgccgcg catgaacaac tggtcgttct
     1861 ggctgctgcc cttcgccttc ctgttgctgc tgctgccgct gatcatctac ctggtgaccg
     1921 gccagggcgc cggcggcccg gccggcggct ggacgctgta tccgccgctg tcgctgcagg
     1981 gcggctccaa cgtcgccacc acgatcttcg cgatccacct gatgggcatc agctcgatca
     2041 tgggtgcgat caacatcatc gcgaccatcc tcaacatgcg cgcgccgggc atggacctgc
     2101 tgaagatgcc gctgttcacc tggacctggc tgatcaccgc gttcctgctc atcgcggtga
     2161 tgccggtgct ggc
//
LOCUS       DAORUK010000243         2132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_81291_length_2132_cov_18.527684, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000243 DAORUK010000000
VERSION     DAORUK010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81291_length_2132_cov_18.527684"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1588
                     /locus_tag="PK818_10865"
     CDS             77..1588
                     /locus_tag="PK818_10865"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HQY83269.1"
                     /translation="MTHWPLLSLLVWLPILGGVFTMMAGNSRPRAARWIALAFAVASL
                     LLCIPLFAGFDLGSGAMQFVERRTWIPTYDIEYHLGADGISLALIGLTTLTTVLVLIG
                     AWTAIDKRVSQYYAALLILEGLMIGVFSALDALLFYVFFEGMLIPMFIIIGVWGGPRR
                     VYASVKFFLYTFLGSVFMLVGLVYLYLKGGSWQLADMAALPLTMAEQTWLFFAFLVAF
                     AVKVPMFPVHTWLPDAHVEAPTGGSVILAAIMLKIGGYGFLRFVLPIVPDAGHHFAWL
                     VITLSLVAVVYVGLVALVQEDMKKLIAYSSVSHMGFVTLGTFIAFALVRDGGNADAAR
                     LGLQGAMVQMVSHGFISGAMFSCVGVLYDRMHSRMIRDYGGVANSMPWFAMFWVLFAM
                     ANSGLPGTSGFVGEFMVILAAFQANPLIAFAAAFTLIIGAGYTLWLTKRVIWGDTGNA
                     HVATLEDINMREWIVLGVFAIGVLVLGVWPKPLIDLMEPSIAQLAGQLAATKL"
     gene            1613..>2132
                     /locus_tag="PK818_10870"
     CDS             1613..>2132
                     /locus_tag="PK818_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004087926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQY83270.1"
                     /translation="MPTVADLFPLLPELVLVGAAFALLMLDLFLDEKQRVLTHVLAIC
                     TLVLVGVLLVAGWGGQGTVLSGMFIRDNMADVLKVGVCFVSAISLVYAWPFLRERGLY
                     KGEVPVLVMFAVAGMMLLISAGSLVMVYLGLELLALCSYALVAIDRDSPLASEAAMKY
                     FVLGALASGMLLY"
BASE COUNT          272 a          678 c          725 g          457 t
ORIGIN      
        1 ctgggcctgg tcgcgctgct cgcgctggcc aaccactact ggctgtgagc cagaccgact
       61 ggatggaatc tcaagcgtga cccactggcc cctcctcagt ttgctggtct ggctgccgat
      121 cctcggcggc gtgttcacca tgatggcggg caattcgcgc ccgcgtgccg cgcgctggat
      181 cgcgctggcg tttgcggtcg caagcctgct gctgtgcatt ccgctgttcg ccggtttcga
      241 cctcggcagc ggggcgatgc agttcgtcga gcggcgcacc tggatcccga cctacgacat
      301 cgagtaccac ctgggcgcgg acggcatctc gctggccctg atcgggctga ccaccctgac
      361 cacggtgctg gtgctgatcg gcgcatggac cgcgatcgac aagcgcgtca gccagtacta
      421 cgcggcgttg ctgatcctcg aaggcctgat gatcggcgtg ttcagcgccc ttgatgcgct
      481 gctcttctac gtgttcttcg agggcatgct gatcccgatg ttcatcatca tcggtgtgtg
      541 gggcggcccg cgccgggtgt atgcctcggt caagttcttc ctgtacacgt tcctcggttc
      601 ggtgttcatg ctggtcggac tggtctacct gtacctgaag ggcggcagct ggcaactggc
      661 ggacatggcc gcgctgccgc tgaccatggc cgaacagacc tggctgttct tcgccttcct
      721 ggtcgcgttc gcggtcaagg tgccgatgtt cccggtccac acctggctgc cggacgcgca
      781 cgtcgaggcg ccgaccggcg gctcggtgat cctggccgcg atcatgctga agatcggcgg
      841 ttacggcttc ctgcgcttcg tgctgccgat cgttcccgac gcaggccatc acttcgcctg
      901 gctggtgatc acgctgtcgc tggtcgcggt ggtctacgtc ggcctggtgg cgctggtgca
      961 ggaggacatg aagaagctga tcgcctattc gtcggtttcg cacatgggtt tcgtcaccct
     1021 cggcaccttc atcgccttcg ccttggtgcg cgatggcggc aacgccgacg ccgcccgcct
     1081 tggcctgcag ggcgcgatgg tgcagatggt cagccacggc ttcatttccg gcgcgatgtt
     1141 ctcctgcgtc ggcgtcctgt acgaccgcat gcacagccgc atgatccggg attacggcgg
     1201 ggtggccaat tcgatgccgt ggttcgcgat gttctgggtg ctgttcgcga tggcgaactc
     1261 cggcctgccg ggcaccagcg gcttcgttgg cgaattcatg gtgatcctgg ccgcgttcca
     1321 ggcaaacccg ctgatcgcgt tcgccgccgc cttcaccctc atcatcggcg ccggctacac
     1381 cttgtggctg accaagcgcg tgatctgggg cgataccgga aatgcccacg tggcgacgct
     1441 ggaggacatc aacatgcgcg agtggatcgt gcttggcgtg ttcgccatcg gcgtgctggt
     1501 gctcggcgtg tggccgaagc cgctgatcga cctgatggaa ccctccatcg cgcaactggc
     1561 gggccagctc gccgcgacca agctctaagg tgccgacccg atgatgcagg acatgcccac
     1621 cgtcgccgac ctgttcccgc tgctgccgga gctggtgctg gtcggcgccg cgttcgcgct
     1681 cttgatgctc gacctgttcc tcgacgagaa gcagcgcgtg ctgacccatg tgctggcgat
     1741 ctgcacgctg gtgctggtcg gggtgctgtt ggtcgcgggc tggggcgggc agggcacggt
     1801 gctgtccggc atgttcatcc gcgacaacat ggccgacgtg ctcaaggtcg gcgtgtgctt
     1861 cgtgtccgcg atctcgctgg tgtatgcctg gccgttcctg cgcgagcgcg gcctgtacaa
     1921 gggcgaggtg ccggtgctgg tgatgttcgc ggtggccggg atgatgctgc tgatctccgc
     1981 cggcagcctg gtcatggtgt acctgggcct ggagctgctg gcgttgtgct cgtacgcgct
     2041 ggtggcgatc gaccgcgaca gcccgctggc ctcggaggcg gcgatgaagt acttcgtgct
     2101 cggcgcgctg gcctcgggca tgctgctgta cg
//
LOCUS       DAORUK010000244         2100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_83335_length_2100_cov_14.792176, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000244 DAORUK010000000
VERSION     DAORUK010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83335_length_2100_cov_14.792176"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="PK818_10875"
     CDS             complement(<1..1350)
                     /locus_tag="PK818_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006452404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQY83271.1"
                     /translation="MPHHTSLIALLCVGFVVAFALGMLAQRLRLSPLVGYLLAGVIVG
                     PFTPGFVADQTLAPQLAEVGVILLMFGVGLHFSLRDLLDVKNIAVPGALLQIAFVTSI
                     GAGFGLLLGWAPIGAVVFGLALSVASTVVVMRALEERRLLDTRRGRIAIGWLVVEDLA
                     MVLALVLLPAITAALGAGEHVGAAGVLGALAWTLLKVAAFVAFMLVVGRRVIPRLLEH
                     VAGLGSRELFTLCVLAIALGVAFGAAALFDVSFALGAFFAGMLLNESELSHKAAADSL
                     PMRDAFAVLFFVSVGMLFNPSILVDQPLLVAATVAVIVFGNATIGFAVVRLFRLPTLT
                     GLTVSASLSQIGEFSFILAGLGLSQKVLPQQGHDLILAGALLSIIANPLLFAWLDRWQ
                     KRQQPGAAVTQVLAENSEVPEHMGGHAIVVGYGRVGRELSRLLQDRGVPLVLVESDPD
                     "
     gene            1458..>2100
                     /locus_tag="PK818_10880"
     CDS             1458..>2100
                     /locus_tag="PK818_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQY83272.1"
                     /translation="MISFRNFALRRGERLLLSNVDLTLHAGWRVGVIGRNGAGKTSLF
                     AALMGEVEPDKGDLDLPGKARIASVAQETPHLPDPAIDFVLSGDAAVHAAVQAEANAF
                     ANEDWEAVAEAHHRLEELNGYDASARAGRLLHGLGFSADTHETPVSDFSGGWRVRLNV
                     ARALMTPSDLLLLDEPTNHLDLDAVVWMEEWLKRYAGTLLVISHDREFLDNVTT"
BASE COUNT          380 a          766 c          702 g          252 t
ORIGIN      
        1 gtccgggtcg ctctccacca gcaccagcgg cacgccgcgg tcctgcagca ggcgcgacag
       61 ctcgcgtccc acccggccgt accccaccac gatggcgtgg ccgcccatgt gctccggcac
      121 ttcggaattt tcggcgagga cctgggtgac ggccgcaccc ggctgctggc gcttctgcca
      181 gcggtccagc cacgcgaaca acagcgggtt ggcgatgatc gacagcagcg cgccggccag
      241 gatcaggtcg tgcccctgct gcggcagcac cttctggctg aggccgaggc cggcgaggat
      301 gaaggagaac tcgccgatct gcgacaggct ggccgagacc gtcaggccgg tcagggttgg
      361 caacctgaac aggcgcacca cggcgaagcc gatggtcgcg tttccgaaca cgatgacggc
      421 cacggtcgcg gccaccagca gcggctggtc gaccaggatc gacgggttga acagcatgcc
      481 caccgagacg aagaacagca ccgcgaacgc atcgcgcatc ggcagcgagt cggcggccgc
      541 cttgtggctg agttccgatt cgttgagcag catgccggcg aagaacgcgc caagcgcgaa
      601 gctgacgtcg aacagcgccg ccgcgccgaa cgccaccccc agcgcgatcg ccagcacgca
      661 cagggtgaac agttcgcgcg agccgaggcc ggccacgtgc tccagcaggc gcgggatcac
      721 ccgccgcccg accaccagca tgaaggcgac gaacgcggcg accttcagca gcgtccaggc
      781 cagcgcgccg agcacgccgg cggcgccgac gtgttcgccc gcgcccagcg cagcggtgat
      841 cgccggcagc agcaccagcg ccagcaccat cgccaggtcc tcgaccacca gccagccgat
      901 cgcgatgcgc ccgcgccggg tatccagcag ccgccgttcc tcaagcgcgc gcatcaccac
      961 cacggtgctg gccaccgaca gcgccagccc gaacaccacc gcgccgatcg gcgcccagcc
     1021 cagcagcagc ccgaagccgg cgccgatcga ggtgacgaag gcgatctgca gcagcgcgcc
     1081 gggcacggcg atgttcttca cgtccagcag gtcgcgcagc gaaaaatgca ggcccacccc
     1141 gaacatcagc aggatcacgc ccacttcggc cagctgcggc gccagggtct ggtcggcgac
     1201 gaagcccggg gtgaacggac cgacgatcac cccggccagc aggtagccca ccagcggcga
     1261 cagccgcagc ctctgggcca gcatgccgag ggcgaacgcc accacgaagc cgacgcagag
     1321 cagggcgatc agcgaggtgt ggtggggcat gcaggctcat tcgacagggt gcccggcatc
     1381 ttcgcatccc gcccggtaac gccgcatgac gaaacgccgc gggcacccgg atggccgccg
     1441 ccgcttacaa tgtcgcgatg atttccttcc gcaatttcgc cctgcgtcgc ggcgaacgcc
     1501 tgctgctgtc caacgtcgac ctcaccctgc acgccggctg gcgggtgggc gtgatcggcc
     1561 gcaacggcgc cggcaagacc agcctgttcg ccgcattgat gggcgaggtg gagcccgaca
     1621 agggcgacct cgacctgccc ggcaaggcgc gcatcgccag cgtggcccag gaaaccccgc
     1681 acctgccgga cccggcgatc gacttcgtgc tgtccggcga cgccgcggtg cacgcagcgg
     1741 tgcaggccga agccaatgcc ttcgccaacg aggactggga agcggtggcg gaagcccacc
     1801 accggctgga agagctgaac ggctacgacg ccagcgcccg cgcgggccgg ttgctgcacg
     1861 ggctgggatt ttccgcggac acccacgaga ccccggtgtc ggatttttcc ggcggttggc
     1921 gggtgcgcct gaacgtggcg cgtgcgctga tgacgccgag cgacctgttg ctgctcgacg
     1981 aacccaccaa ccacctcgac ctcgacgcgg tggtgtggat ggaggaatgg ctgaagcgct
     2041 acgcgggcac cctgctggtg atctcgcacg accgcgagtt cctcgacaac gtcaccaccc
//
LOCUS       DAORUK010000245         2090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_83950_length_2090_cov_22.835872, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000245 DAORUK010000000
VERSION     DAORUK010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83950_length_2090_cov_22.835872"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PK818_10885"
     CDS             complement(<1..632)
                     /locus_tag="PK818_10885"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017171771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HQY83273.1"
                     /translation="MPAPNVVILAGGTGGHIFPGLAVAHALRARGANVGWLGADGGME
                     TRLVPQHGLAIDTIAVSGLRGKGIATLLSAPLRVLRAVRDAARVLRLRKPDAVVSFGG
                     YAAGPGGIAARIAGIPLLVHEQNRAAGMTNKVLAKFARQVLVGFPQTFANETLVGNPV
                     RAQIAQALPPAQRDFHHGGPLRLLVLGGSQGARALNNAVPQAVAALGQPV"
     gene            complement(641..1984)
                     /gene="ftsW"
                     /locus_tag="PK818_10890"
     CDS             complement(641..1984)
                     /gene="ftsW"
                     /locus_tag="PK818_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HQY83274.1"
                     /translation="MHDGRRQAMRVDDIGGRFDPWLLGVSIALACFGVVMVGSAAVAG
                     AGMDVGPWYFLTRHVLFLAGGLVLAAFAMRMELKVVEQHSQLLLLACFALLLVVFVPH
                     VGSSVNGARRWINLGISKFQVVEAVKVLYIVWLASYLKRYSEDVAVTWMAMLKPVGVV
                     GALVVLLLLQPDFGSSVLLLGITTCMLLLGGAPIRRITLPILLLVPALVLLVVFEPYR
                     MRRVTSFMDPWQDQLGAGYQLSNALMAIGRGEWFGVGLGASVQKLNYLPEAHTDFIFS
                     VIAEETGFAGVCLVVGMYALLAGRAFWLGLRCVEMKRHFAGYIAFGIALWMALQSFVS
                     IGVNLGLLPTKGLTLPLVSSGGSSVLMTCAALGLLLRVSYEYERAARQVAKLRGVATG
                     PSVGSPPPTSQPSMSQAPKPGEPAPRGTSRLRQRVEPSMGTATESAAAAALRRSR"
BASE COUNT          368 a          767 c          701 g          254 t
ORIGIN      
        1 tcgaccggct ggcccagcgc cgcgaccgcc tgcggcaccg cgttgttgag cgcacgcgcg
       61 ccctggctgc cgcccagtac cagcaggcgc agcggaccgc cgtggtggaa gtcgcgttgt
      121 gccggcggca acgcctgcgc gatctgcgcg cgcaccggat tgccgaccag ggtttcattg
      181 gcgaaggtct gcgggaagcc gaccagcacc tgccgcgcga acttcgccag caccttgttg
      241 gtcatgccgg cggcgcggtt ctgttcatgc accagcagcg ggatgccggc gatccgcgcg
      301 gcgatcccgc ccgggcccgc ggcataccct ccgaagctca ccaccgcgtc cggcttgcgc
      361 aggcgcagca cgcgggcggc gtcgcgcacc gcgcgcagca cccgcaacgg cgccgacagc
      421 agcgtggcga tgcccttgcc gcgcaatccc gacaccgcga tggtgtcgat cgccagccca
      481 tgctgcggca ccagccgggt ttccatgccg ccatccgcgc ccagccagcc gacattcgcg
      541 ccacgcgcac gcaacgcatg cgcgaccgca aggccgggga agatgtggcc gccggtgccg
      601 ccggccagga tgaccacgtt cggcgcgggc aacggcgtgc tcaacgcgac ctccgcaatg
      661 cggcggcggc ggcggattcg gtggcggtgc ccatgctcgg ctcgacccgc tggcgcagcc
      721 ggctggtgcc gcgcggcgcc ggctcgccgg gcttcggtgc ctggctcatg gagggttgcg
      781 aggtcggcgg cggactgccc accgacgggc cggtggccac cccgcgcagc ttcgcgacct
      841 ggcgcgcggc gcgctcgtat tcgtaggaca cccgcagcag caggcccagc gcggcgcacg
      901 tcatcagcac gctggaacca ccggaggaga ccagcggcag ggtcaggccc ttggtcggca
      961 gcaggccgag gttgaccccg atcgagacga agctttgcag cgccatccac agggcgatgc
     1021 cgaaggcgat gtagccggcg aagtggcgct tcatctcgac gcacctcagg cccagccaga
     1081 acgcgcgccc ggccagcagc gcatacatgc cgacgacaag gcacacgcca gcgaagccgg
     1141 tctcctcggc gatcaccgag aagatgaagt cggtatgcgc ttccggcagg tagttgagct
     1201 tctgcaccga tgccccaagc ccgaccccga accactcgcc gcggccgatc gccatcagcg
     1261 cattgctgag ctggtaaccg gcgccgagct ggtcctgcca cggatccatg aacgaggtga
     1321 cccggcgcat gcggtacggt tcgaacacca ccagcagcac cagcgccggc accagcagca
     1381 ggatcggcag ggtgatgcgg cggatcgggg cgccgcccag cagcaacatg caggtggtga
     1441 tgcccagcaa cagcaccgag gaaccgaagt ccggctgcag cagcagcagc acgaccagcg
     1501 cgccgaccac gccgaccggc ttgagcatcg ccatccaggt gaccgccacg tcctcgctgt
     1561 aacgcttgag gtagctcgcc agccacacga tgtagaggac cttgaccgct tccaccacct
     1621 ggaacttgga gatgccgagg ttgatccagc gccgagcgcc gttgaccgaa ctgcccacgt
     1681 gcgggacgaa caccaccagc agcagggcga agcaggccag cagcagcagc tggctgtgct
     1741 gttcgacgac cttcagttcc atgcgcatgg cgaaggcggc cagcaccagt ccgccggcga
     1801 ggaacagcac gtggcgggtc aggaagtacc acgggccgac gtccatgccg gcaccggcga
     1861 ccgccgccga gccgaccatc accaccccga agcaggccag cgcgatcgac acccccagca
     1921 gccacgggtc gaagcgcccg ccgatgtcgt cgacgcgcat cgcctggcgc ctgccgtcgt
     1981 ggatcgcgct gtcgaaggcg atggcgccca tcagcggacc ttcagggtcg caaggccgac
     2041 cagcaccagc accacgctga tgatccagaa gcgcacgatc acccgcggct
//
LOCUS       DAORUK010000246         2051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_86627_length_2051_cov_22.540581, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000246 DAORUK010000000
VERSION     DAORUK010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86627_length_2051_cov_22.540581"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PK818_10895"
     CDS             <1..609
                     /locus_tag="PK818_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HQY83275.1"
                     /translation="GIGAFSCALIMAPVLNLLAQAYGIGDPTRPGSLQAPQATLMASV
                     AQGLFGGKLPWDMIAAGGVIGAIVIAFDGWLKSRNSTFRVPVLAAAIGIYLPLELMVP
                     IFLGGLLAYFVEKRHGIVGTHDEPLRDRVHRPGVLFSAGLITGEALMGIAIAIPIVVS
                     ERADVLALPEQFHFGQFVGLVVLALVGWLIYRTGVKANPHAA"
     gene            673..>2051
                     /locus_tag="PK818_10900"
     CDS             673..>2051
                     /locus_tag="PK818_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017169463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQY83276.1"
                     /translation="MKLRHAILPLALALSLPAVADAARGFSVRDMAYLDRHSAPTLSP
                     DGRVLVFAKRVVDKDTNKSGTSLWIRNLATRDMRPPQRLTPDGWNVNSPAFSADGKSV
                     HFLSAKSGSMQLYSIPVAGGEPKRLTDTVLDVDGYKLSPDGTQVALAFAVFPECKADL
                     DCTKKKLDAAEAAKATGTVFDRMFIRHWDAWNDGRLNRVFAAKLGGDGMLKAATLLSG
                     GIAGDIPSKPFGDLSDFAWSADGKQVAMSVRQANREEPWNTNFDIWLVNADGTGARNL
                     TAGNPAWDAGPVFSTDGKTLYYRAMKRPGFEADRFALMAMALGSGKAREIAPAWDRSA
                     DGITLSADGRAIYTTAADLGQHPLFAVDIASGTASKVIGGGSISSFDIAGGTLAFSRN
                     SLESGDVIATTSVDGKAPVRAITPSAGEMLPEVAWGAYEQFNFTGWNNETVHGYVVKP
                     WNYQPGRKYP"
BASE COUNT          321 a          706 c          698 g          326 t
ORIGIN      
        1 ggcatcggcg cgttctcctg cgcgctgatc atggcgccgg tgctgaacct gctggcgcag
       61 gcctacggca tcggcgaccc cacccgtccg ggttcgctgc aggcgccgca ggccacgctg
      121 atggcctcgg tggcgcaggg attgttcggc ggcaagctgc catgggacat gatcgcggcg
      181 ggcggcgtga tcggcgcgat cgtgatcgcg ttcgacggct ggctgaagtc gcgcaattcg
      241 acgttccgcg tgccggtgct ggccgcggcg atcggcatct acctgccgct ggaactgatg
      301 gtgccgatct tcctcggcgg cctgctggcg tatttcgtcg agaagcgcca cggcatcgtc
      361 ggcacccacg acgaaccgct gcgcgatcgc gtgcatcgcc ccggcgtgct gttctccgcc
      421 ggcctgatca ccggcgaggc gctgatgggc atcgccatcg cgatcccgat cgtggtcagc
      481 gaacgcgcgg acgtgctggc gctgccggaa cagttccact tcggccagtt cgtcggcctg
      541 gtggtgctgg ccctcgtcgg ctggctgatc taccgcaccg gcgtgaaggc caacccgcac
      601 gccgcctgat ccatccgcgc ccgcggatcg ccgcgggctc tccactatcc cgaaagacgg
      661 aacccgcccg ccatgaaact ccgccacgcc atccttccgc ttgcccttgc gctgtcgctg
      721 cccgcggtcg ccgatgccgc gcgcggcttc agcgtgcgcg acatggccta ccttgaccgc
      781 cactcggcgc cgaccctgtc gccggacggg cgcgtgctgg tgttcgccaa gcgggtggtg
      841 gacaaggaca ccaacaagtc cggcacctcg ctgtggatcc gcaacctggc gacgcgcgac
      901 atgcggccgc cgcagcggct gaccccggac ggctggaacg tgaattcgcc ggcgttttcg
      961 gccgatggca aatccgtcca cttcctgagc gcgaagtcgg gttcgatgca gctgtattcg
     1021 atcccggtgg caggcggtga accgaagcgg ctgaccgaca ccgtgcttga tgtcgatggc
     1081 tacaagctct cgcccgatgg cacccaggtg gcgctcgcat tcgcggtgtt cccggagtgc
     1141 aaggcggacc tcgactgcac gaagaagaag ctcgacgccg ccgaggcagc caaggccacc
     1201 ggcacggtct tcgaccgcat gttcatccgc cactgggatg cctggaacga cggccgcctg
     1261 aaccgcgtgt tcgcggcgaa gctgggtggc gacggcatgc tcaaggcggc aacgttgctg
     1321 agcggcggca tcgccggcga catcccgtcc aagccgttcg gcgacctgtc cgatttcgcc
     1381 tggtcggcgg atggcaagca ggtcgcgatg agcgtgcgcc aggccaaccg cgaggagccg
     1441 tggaacacca atttcgacat ctggctggtc aacgcggatg gcactggcgc gcgcaacctc
     1501 accgcgggaa atccggcatg ggatgcgggc ccggtgttca gcaccgacgg caagaccctg
     1561 tactaccgcg cgatgaagcg cccgggcttc gaggccgacc gtttcgcgct gatggcgatg
     1621 gcgctggggt ccggcaaggc gcgcgaaatc gcgccggcgt gggaccgcag cgccgacggc
     1681 atcaccctgt ccgccgatgg ccgggcgatc tacaccaccg ccgccgacct cggccagcat
     1741 ccgctgttcg cggtggacat cgccagcggc accgccagca aggtgatcgg cggcggcagc
     1801 atttccagct tcgacatcgc cggcgggacg cttgcgttct cgcgcaactc gctggagagc
     1861 ggcgacgtga tcgccaccac cagcgtcgat ggcaaggcac ccgttcgcgc gatcacgccc
     1921 agcgccggcg agatgttgcc ggaggtggcc tggggggcgt acgagcagtt caacttcacc
     1981 ggctggaaca acgagaccgt gcatggctac gtggtcaagc cgtggaacta ccagccgggc
     2041 aggaagtacc c
//
LOCUS       DAORUK010000247         2005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_90061_length_2005_cov_19.505128, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000247 DAORUK010000000
VERSION     DAORUK010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90061_length_2005_cov_19.505128"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2005
                     /locus_tag="PK818_10905"
     CDS             <1..>2005
                     /locus_tag="PK818_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HQY83277.1"
                     /translation="QSRRLVGGDAAFSSDDRAIDDARRHAQRLQLRLFRSVELASMVG
                     SRTAPPQLGTLKALAADAPLLGPYSVRTARGVQRLARPAAGTLWLSETGAQRMRTPLG
                     GIVEVGGRPLRLAGIVVEEPDRPLNGVELGPRVILPLAEVEAGGLLGPGARASWRIAV
                     SGAPAAIAEWVKAREADRSPGARVETGDDLNPQLRAALDRAQRFLSVSLVLTAALAAV
                     AIGMAARQHAERHRQIAATFRALGARQGDIVGLYVGTLAILGGIAILAALLAAAVLQQ
                     VAGAWIAEQLGATLPPAKLLPLLQGALVGVWILVTFALPPLLALRRVSALAVLRSDLR
                     TPSPPALALFALAMLGLLALFWQAAGNAQAATILLGGLLATTFVLGVAGWALAALVAR
                     AGRGGSGILRYALLNLSRRRRLTVAQVVALGTSLMALLLLLLVRTDVWEQWQETVRGD
                     APNRFVINVQPEQRPAFDAVLDRLDYASVGLSPLIRARYVGASAQQRGRPAPQGRGER
                     LADREFNLSTSDVLPDGNTLKAGKFWSAATARNEFSVETEFAEQLRWRLGDKVAFDVA
                     GRRIEGTITSLRGVRWESFTPNFFVLASPDLARDLPATYITAVRVPQGDTALASALSQ
                     AVPNANVIDIDAITAEIRRIGDQAALVIQVVFWFAFLSGCLVFV"
BASE COUNT          237 a          729 c          739 g          300 t
ORIGIN      
        1 aacagagccg gcggctggtc ggcggcgacg ccgcgttcag cagcgacgac cgcgccatcg
       61 atgatgcccg ccggcatgcg caacgcctgc agttgcgcct gttccgcagc gtggagctgg
      121 ccagcatggt cggctcgcgc acggcgccgc cgcagctcgg cacgctgaag gcgctggccg
      181 ccgatgcccc gctgctcggg ccgtacagcg tgcgcaccgc gcggggcgtg cagcggctgg
      241 cccgccccgc cgccggcacc ctgtggctga gcgaaaccgg cgcgcaacgg atgcgcacgc
      301 cgctgggcgg gatcgtcgag gtcggcggca ggccgttgcg gctggccggg atcgtggtcg
      361 aggaacccga ccgtccgctc aacggcgtgg aactcgggcc gcgggtgatc ctgccgctgg
      421 ccgaggtcga agccggcggc ctgctcggcc ccggcgcgcg cgcctcgtgg cggatcgcgg
      481 tcagcggcgc gccggccgcg atcgccgaat gggtgaaggc gcgcgaagcc gaccgcagcc
      541 ccggcgcgcg ggtggagacc ggcgacgacc tcaatccgca gttgcgcgcg gcgctggatc
      601 gcgcgcaacg cttcctgtcg gtgtcgctgg tgctgaccgc ggcgctggcc gcggtggcga
      661 tcggcatggc cgcgcgccag catgcggagc gccaccgcca gatcgccgcc accttccgtg
      721 cgctgggtgc gcggcagggc gacatcgtcg gcctgtacgt cggcaccctg gcgatcctcg
      781 gcggcatcgc gatcctcgcc gcgctgctcg cggcggcggt gctgcagcag gtcgcgggtg
      841 cgtggatcgc ggaacagctc ggcgcgacac tcccgcccgc caagctgctg ccgctgttgc
      901 agggcgcgct ggtcggcgtg tggatcctgg tcaccttcgc gctgccgccg ttgctcgcgc
      961 tgcgccgggt cagcgcgctg gcggtgctcc gcagcgacct gcgcacgccc tcgccgcccg
     1021 cgcttgcgct gttcgcgctg gcgatgctcg gcctgctggc gctgttctgg caggccgccg
     1081 gcaatgcgca ggccgcgacc atcctgcttg gcggcctgct ggccaccacc ttcgtgctgg
     1141 gcgtggccgg ctgggcgctg gccgcactgg tcgcgcgcgc cggccgcggc ggcagcggca
     1201 tcctgcgcta tgcgttgctc aacctgtcgc ggcgccgccg gctgacggtg gcgcaggtgg
     1261 tcgcactcgg caccagcctg atggcgctgc tgctgttgct gctggtgcgc accgatgtgt
     1321 gggagcagtg gcaggagact gtgcgcggcg atgccccgaa ccgtttcgtg atcaacgtgc
     1381 agcccgaaca acggccggcg ttcgatgcgg tgctggaccg gctcgactac gcctcggtcg
     1441 ggctgtcgcc actgatccgc gcccgctatg tcggcgccag tgcgcagcag cgtggtcgcc
     1501 ccgcgccgca gggccgcggc gagcgcctgg ccgatcgcga attcaacctc tccacgtccg
     1561 acgtgctgcc ggacggcaac acgctgaagg ccggcaagtt ctggagcgcg gcgactgcgc
     1621 gcaacgagtt ctcggtggaa accgaattcg ccgagcagct gcgctggcgg ctgggcgaca
     1681 aggttgcatt cgacgtcgcc ggccgccgca tcgaaggcac catcaccagc ctgcgcggcg
     1741 tgcgctggga gagcttcacc ccgaacttct tcgtgctcgc ctcgcccgac cttgcccgcg
     1801 acctgcccgc cacctacatc accgcggtac gggtgccgca gggtgacacc gcgctggcca
     1861 gcgcactctc gcaagcggtg ccgaacgcga acgtgatcga catcgacgcg atcaccgccg
     1921 agatccgccg gatcggcgac caggccgcgc tggtgatcca ggtggtgttc tggttcgcct
     1981 tcctgtccgg ctgcctggtg ttcgt
//
LOCUS       DAORUK010000248         1996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_90742_length_1996_cov_16.266358, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000248 DAORUK010000000
VERSION     DAORUK010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90742_length_1996_cov_16.266358"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PK818_10910"
     CDS             <1..474
                     /locus_tag="PK818_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQY83278.1"
                     /translation="YEQAGIAKDGAKMVTAVACSHVPKFTVVIGGSFGAGNYAMCGRA
                     YGARFLWMWPNARISVMGGEQAASVLATVKRDGIEARGGVWTPDEEDAFKAPIRQQYE
                     DQGNPYYATARLWDDGIIDPADTRRVLGLGLSASLNAPIESGPGRDGTRFGVFRM"
     gene            581..1072
                     /locus_tag="PK818_10915"
     CDS             581..1072
                     /locus_tag="PK818_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HQY83279.1"
                     /translation="MAIWKDPTPAVKPEPTRFDAPSPVHDAAHASPVPTPIRAVDSPA
                     RESVIAADLAIEGKIEGAGHVRIAGRFKGDVKVQGNLTIEQGAKVAGGVAASKVTLAG
                     ELDGNIESAQQVELLQGGVINGDVKAGSLTVAAGSRIRGHIECGWDKDGKSTPKTEPG
                     SAA"
     gene            1069..1425
                     /locus_tag="PK818_10920"
     CDS             1069..1425
                     /locus_tag="PK818_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83280.1"
                     /translation="MSAVRPGATGATRTCPHCKATILESAAVCPACRGHLKFEAAALD
                     AAQRFVPLKVEGTINAADDGAFEYTMVVVIRDQRGKEIGRHVAGVGAVFPGESRSFEV
                     SVEAVAAAPGKRRLKH"
     gene            complement(1429..1662)
                     /locus_tag="PK818_10925"
     CDS             complement(1429..1662)
                     /locus_tag="PK818_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83281.1"
                     /translation="MQAGLLVQYLAVALAVLASAGVVMHKQFPNATRRLRIATALPLL
                     REGSPRWAKALGRVIAPAARTGGKDCGGCDGCD"
     gene            complement(1665..>1996)
                     /locus_tag="PK818_10930"
     CDS             complement(1665..>1996)
                     /locus_tag="PK818_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020851591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HQY83282.1"
                     /translation="RISIALIPTMAAREVMVSSLATVYAVAASSEEAASLALEPLIAQ
                     QWSLATGLSLLVWFIYAPQCISTLATIRRETHSWKQTAFAAGYLFALAYLASLLTYQV
                     ALAFGAG"
BASE COUNT          340 a          694 c          708 g          254 t
ORIGIN      
        1 tacgagcagg ccggcatcgc caaggacggg gcgaagatgg tgaccgcggt ggcgtgcagc
       61 catgtgccca agttcaccgt ggtgatcggc ggcagcttcg gtgccggcaa ctacgcgatg
      121 tgcggccgcg cctacggcgc gcgcttcctg tggatgtggc cgaacgcgcg catttcggtg
      181 atgggcggcg agcaggcggc cagcgtgctg gccacggtga agcgggacgg catcgaggcc
      241 cgcggcgggg tgtggacgcc ggacgaggaa gacgccttca aggcaccgat ccgccagcag
      301 tacgaggacc agggcaaccc ctactacgcc accgcgcggc tgtgggacga cggcatcatc
      361 gatcccgccg acacccgccg ggtgctgggg ctgggactgt cggccagcct caacgcgccg
      421 atcgaaagcg ggcccggtcg cgacggcacc cgcttcggcg tgttccggat gtgatccggc
      481 ccacgatcag ccacttgcag cacctacctg ccgcattcat gcaggaaatc gtcgcaaagt
      541 agtgctagcc tcggcggtcc gcccccgagg acctgctgcc atggccatct ggaaagaccc
      601 gacgccggcc gtcaagcccg aacccacgcg cttcgatgca ccctcgcccg tgcacgatgc
      661 ggcccatgca tcgcctgtgc cgaccccgat ccgcgcggtg gacagcccgg cacgcgaatc
      721 ggtgatcgcc gccgacctcg ccatcgaggg caagatcgaa ggcgccggcc atgtccgcat
      781 cgccggccgg ttcaagggcg acgtcaaggt gcagggcaac ctgaccatcg aacagggcgc
      841 caaggtcgcc ggcggcgtgg ccgcgagcaa ggtgaccctg gccggcgagc tggacggcaa
      901 catcgaatcg gcgcagcagg tcgaactgct gcagggcggc gtgatcaacg gcgatgtcaa
      961 ggccggttcg ctgaccgtag ccgccggctc gcgcatccgc ggccacatcg aatgcggctg
     1021 ggacaaggac ggcaagtcga cgccgaagac ggagcccggc agcgccgcat gagcgcggtg
     1081 cgcccgggcg cgacgggtgc cacccgcacc tgcccgcatt gcaaggccac gatcctggaa
     1141 agcgcggcgg tctgcccggc ctgccgcggg cacctgaagt tcgaggccgc ggcgctggac
     1201 gcggcgcaac gcttcgtgcc gctgaaggtg gaaggcacga tcaacgccgc cgacgacggc
     1261 gcgttcgagt acaccatggt ggtggtgatc cgcgaccagc gcggcaagga gatcggccgc
     1321 cacgtggctg gcgtcggtgc ggtgttcccc ggcgaatcgc gcagcttcga ggtcagcgtg
     1381 gaagcggtgg cggccgcgcc gggcaagcgc cggctgaagc actgaacctc agtcgcagcc
     1441 gtcgcaaccg ccgcaatcct tgccgccggt gcgtgccgcc ggcgcgatga cccgccccag
     1501 cgctttcgcc caacgcggcg acccctcgcg cagcaacggc agcgcggtcg cgatccgcag
     1561 cctgcgggtg gcgttcggga actgcttgtg catcaccacg ccggcactgg cgagtaccgc
     1621 cagcgcgacc gcgaggtact gcaccagcag tcccgcctgc atgctcagcc cgccccgaac
     1681 gcgagcgcga cctggtaggt gagcagcgac gccaggtagg ccagcgcgaa caggtaaccg
     1741 gcggcgaacg cggtctgctt ccacgaatgg gtttcgcggc ggatcgtggc cagggtggag
     1801 atgcactgcg gcgcatagat gaaccagacc agcagcgaca ggccggtggc cagcgaccac
     1861 tgctgcgcga taagcggttc cagcgcgagg ctggcggcct cctcgctgga tgccgccacc
     1921 gcgtacaccg tggccagcga cgacaccatc acctcgcgcg cggccatggt cgggatcagc
     1981 gcgatgctga tccgcc
//
LOCUS       DAORUK010000249         1993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_90977_length_1993_cov_16.340041, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000249 DAORUK010000000
VERSION     DAORUK010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90977_length_1993_cov_16.340041"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="PK818_10935"
     CDS             complement(<1..1520)
                     /locus_tag="PK818_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQY83283.1"
                     /translation="MNDSHPNPASAPADTQAPDERLRQALALLEAIAADRTVLDGCPE
                     DQRARLHRAIAAIYHPEPKLRRQKTKERERERNAEAIRRADALLDSTGIRELRRKPVF
                     TTPNYFPPQGFDALGNPQSTNADGTTQAGDASRESGELLHCYVCKQKYTRVHHFYDQL
                     CPACAGFNFAKRTELADLTGRVALLTGGRVKIGYQAGLKLLRAGARLIVTTRFPRDSA
                     ARYAQEPDFGEWGHRLEVFGLDLRHTPSVEAFCTELAAKLPRLDFIINNACQTVRRPP
                     GFYAHMMAAETAALRDTPEHVRNLLGSYEGLRSYTMLPEGDAASLQVAIGSERLAEAA
                     GLTRPAELSQVPLLAEELLGQEHLFPEGRLDQDLQQVDLRGRNSWRLLMHEVPSVELL
                     ETQLVNAIAPFIINARLKPLMLRTPERDKHIVNVSAVEGQFYRNFKTTRHPHTNMAKA
                     ALNMMTRTAAADYHGDGIHMNSVDTGWVTDEDPAEIAARKVVEERFHPPLDIVDGAA"
     gene            complement(1637..>1993)
                     /locus_tag="PK818_10940"
     CDS             complement(1637..>1993)
                     /locus_tag="PK818_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HQY83284.1"
                     /translation="KNIVAAGLADKCEVQVSYAIGVAEPTSISVTTFGTGKIADAEIE
                     KLIRAHFDLRPYGIVKMLDLIHPIYQQTAAYGHFGRKPKDVSYTDGAGNKQTATAFSW
                     EKTDKADDLRKAAKLK"
BASE COUNT          258 a          627 c          738 g          370 t
ORIGIN      
        1 gcggcgccgt cgacgatgtc cagcggcgga tggaagcgtt cctcgaccac cttgcgcgcc
       61 gcgatctcgg ccggatcctc gtcggtgacc cagccggtgt cgacgctgtt catgtggatg
      121 ccgtcgccgt ggtaatccgc ggcggcggtg cgggtcatca tgttcaacgc ggccttggcc
      181 atgttggtgt gcgggtgacg ggtggtcttg aagttgcggt agaactggcc ctccaccgcc
      241 gacacgttga cgatgtgctt gtcgcgttcc ggggtgcgca gcatcagcgg cttcagccgc
      301 gcattgatga tgaacggcgc gatggcgttg accagctggg tttccagcag ctccaccgac
      361 ggcacctcgt gcatcagcag gcgccacgaa ttgcggccgc gcaggtcgac ctgctgcagg
      421 tcctggtcca gccggccctc ggggaacagg tgctcctggc ccagcagttc ctcggccagt
      481 agcggtacct gcgagagttc ggccgggcgg gtcaggccgg ccgcttcggc cagccgctcg
      541 ctgccgatcg ccacctgcag cgacgcggcg tcgccctccg gcaacatggt gtaactgcgc
      601 aggccctcgt agctgccgag caggttgcgc acatgctcgg gggtgtcgcg cagcgccgcg
      661 gtttccgccg ccatcatgtg cgcgtagaac cccggcgggc ggcgcaccgt ctggcaggcg
      721 ttgttgatga tgaagtccag gcgcggcagc ttggccgcaa gttccgtgca gaaggcctcg
      781 acgctgggcg tgtggcgcag gtccaggccg aacacttcca gccggtggcc ccattcgccg
      841 aagtcgggct cctgcgcgta gcgcgcggcg gagtcgcgcg ggaagcgggt ggtgacgatc
      901 agccgcgcgc ccgcgcgcag cagcttgagg ccggcttgat agccgatctt gacccgaccc
      961 ccggtcagca gcgcgacccg gccggtcagg tcggccaact cggtgcgctt ggcgaagttg
     1021 aagcccgcgc aggcggggca cagctggtcg tagaagtgat ggacccgcgt gtacttctgc
     1081 ttgcacacgt agcagtgcag cagttcgccc gactcgcgcg atgcgtcgcc cgcctgcgtg
     1141 gtgccgtcgg cgttcgtgga ttgaggattg ccgagcgcgt cgaagccctg cggtgggaaa
     1201 tagttcggcg tggtgaacac cggctttcgc cgcagttcgc ggatgccggt gctgtcgagc
     1261 agggcatcgg cgcggcggat ggcttcggcg ttgcgttcgc gttcgcgttc cttggtcttc
     1321 tgccggcgca gcttgggttc cgggtggtag atcgccgcga tggcccggtg caggcgcgcg
     1381 cgttggtcct ccgggcagcc atcgagcacg gtgcggtcgg cggcgatcgc ttccagcaat
     1441 gccagcgcct ggcgcaggcg ctcgtcgggc gcttgcgtgt cggcgggtgc ggaagcgggg
     1501 ttggggtggc tgtcgttcaa cgtcgatcct gttgcgggtg tggcggcgcg caaggcacgc
     1561 atcgaaaagg aaacgggccg ccaagggcgg cccgtccggg actgcgtcgc accctggcag
     1621 gcaggaggcg cgcgggttac ttcagcttcg ccgccttgcg cagatcgtcg gccttgtcgg
     1681 tcttttccca gctgaacgcg gtcgcggtct gcttgttgcc ggcgccgtcg gtgtagctga
     1741 cgtccttcgg cttgcggccg aagtggccgt aggccgcggt ctgctggtag atcgggtgga
     1801 tcaggtcgag catcttgacg atgccgtacg ggcgcaggtc gaagtgcgcg cggatcagct
     1861 tctcgatctc cgcgtccgcg atcttgccgg tgccaaaggt ggtgaccgag atgctggtgg
     1921 gctcggcgac gccgatcgcg tagctgacct gcacctcgca cttgtcggcc aggccggcgg
     1981 cgacgatgtt ctt
//
LOCUS       DAORUK010000250         1964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_93208_length_1964_cov_17.171294, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000250 DAORUK010000000
VERSION     DAORUK010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93208_length_1964_cov_17.171294"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PK818_10945"
     CDS             complement(<1..256)
                     /locus_tag="PK818_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011409164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HQY83285.1"
                     /translation="MNPVDSPSPQRAAVLAFLQRELAAGRAPSLAEIALAFGFASRNA
                     ARKHVQALVADGLLEQTTGRSRGLRLPGGPADLLPLAVLGR"
     gene            complement(338..946)
                     /locus_tag="PK818_10950"
     CDS             complement(338..946)
                     /locus_tag="PK818_10950"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HQY83286.1"
                     /translation="MPEAAAALQRCFWVDGSDLYAAYHDHEWGRPVSDDIRLFEKISL
                     EGFQAGLSWITILRKREHFRAGFDGFDFHRVAAYTEADVQRLLADAGIVRHRGKIEAV
                     IHNAGRAIELQRESGSLARYFRQFEVDVSGRPEKLTVDVLRSFTTAPEAIALSKDLKK
                     RGWKFVGPTTMYAFMQAMGLVNDHQHDCHVRAEIEALRRAAA"
     gene            1086..1280
                     /locus_tag="PK818_10955"
     CDS             1086..1280
                     /locus_tag="PK818_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83287.1"
                     /translation="MFRIQSPSPMAYVERRLTPRRGDGGAQPARIHRERDFGVGYGNS
                     SGYASNRSYAVPAAAYFRCR"
     gene            complement(1459..>1964)
                     /locus_tag="PK818_10960"
     CDS             complement(1459..>1964)
                     /locus_tag="PK818_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQY83288.1"
                     /translation="YAIDAFERGAIDYLLKPVKADRLEATVARLQARDALPDAAALAA
                     LLDKLGALPNAAGAVEPLTWLTASAGRETRLVLVDDVAYFRADHKYTTVVTAEGEALL
                     RTPLRELLPRLDPANFKQIHRSTIVNLKAIAGIVRDDSGRGTVRLKHRPETLTVSQPF
                     MALFRHM"
BASE COUNT          292 a          692 c          678 g          302 t
ORIGIN      
        1 cccggcccag caccgccagc ggcaacaggt ccgccggccc gcccggcagt cgcaggccgc
       61 gcgagcgccc cgtggtttgc tccagcaggc cgtcggcgac cagcgcctgc acatgcttcc
      121 gggcggcatt gcgcgaggcg aagccgaatg ccagcgcgat ctccgccagg ctgggcgcgc
      181 gcccggccgc cagctcccgc tgcaggaagg cgagcacggc ggcgcgttgc ggggacgggg
      241 agtcgacggg gttcatgggt gtacatttgt acaccacggc gtcgcacaag gcgagaccgc
      301 cagcgccggc cccgcaacga cgcgggttgg cggtgcctca ggccgcggcg cggcgcaagg
      361 cctcgatctc ggcgcgcaca tggcaatcgt gctggtggtc gttgaccagc cccatcgcct
      421 gcatgaaggc gtacatcgtg gtcgggccga cgaacttcca gccgcgcttc ttcaggtcct
      481 tcgacagcgc gatcgcctcc ggcgcggtgg tgaagctgcg cagcacgtcc acggtgagtt
      541 tctccgggcg cccggacacg tccacttcga actgccggaa ataacgcgcc agcgaaccgg
      601 attcgcgctg cagctcgatc gcgcggccgg cgttgtggat caccgcctcg atcttgccgc
      661 gatggcggac gatgccggca tcggccagca ggcgctgcac gtcggcctcc gtgtaggcgg
      721 cgaccctgtg gaaatcgaaa ccgtcgaaac cggcgcggaa gtgttcgcgc ttcctgagga
      781 tggtgatcca gctcagcccg gcctggaagc cttccaggct gatcttctcg aacaggcgga
      841 tgtcgtcgct gaccggacgg ccccattcgt ggtcgtggta ggcggcgtac aggtcggaac
      901 cgtcgaccca gaaacagcgt tgcaacgcgg cggccgcttc cggcatctct tgctcctgca
      961 gggagcgtgc aggttagctg aacgattccc atcgaatgcg acgcgcatga cttcctgccc
     1021 atgcaatccg ttgtaatggt gttatacatt accaacacac taagaaacca gcacaggaga
     1081 ccgtcatgtt ccgcatccag tcccccagcc cgatggccta cgtcgagcgc cgcctgaccc
     1141 cgcgccgcgg ggacggcgga gcacagccgg cccgcatcca ccgcgaacgc gacttcggcg
     1201 tcggctacgg caacagcagt ggctatgcca gcaaccgcag ttacgcggtg ccggccgccg
     1261 cctacttccg ctgccgttga gtcgtggcaa cccggccggg tcgcgacctc cccgcgcggc
     1321 ccggccggac ttgtcggcac ccagccacgg cgcgcgctgc gcgcactgcg gctcggagag
     1381 gcctcgaagc acgcgccccg ctgcacggcg gggcgccggt cgatcgtcgc cggttgccgc
     1441 gcgctttcgc gcggcgggtc acatgtgcct gaacagggcc atgaacggct ggctgacggt
     1501 cagcgtttcc gggcggtgtt tcaggcgcac cgtgccgcgg ccgctgtcgt cgcggacgat
     1561 gccggcgatc gccttcaggt tgacgatggt cgaacggtgg atctgcttga agttggccgg
     1621 atcgagccgc ggcagcagct cccgcagcgg cgtgcgcagc agcgcctcgc cttcggcggt
     1681 gaccaccgtg gtgtatttgt ggtcggcgcg gaagtaggcg acgtcgtcga ccagcaccag
     1741 ccgggtttcg cggccggcgc tggcggtcag ccaggtcagc ggctccaccg cgccggcggc
     1801 gttcggcaac gcgcccagct tgtcgagcag ggccgccagc gcggctgcgt cgggcagcgc
     1861 gtcgcgcgcc tgcaggcgtg ccaccgtcgc ctccagccgg tccgccttca ccggcttcag
     1921 caggtagtcg atcgcgccgc gctcgaaggc gtcgatcgcg tact
//
LOCUS       DAORUK010000251         1930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_96040_length_1930_cov_19.264533, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000251 DAORUK010000000
VERSION     DAORUK010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96040_length_1930_cov_19.264533"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PK818_10965"
     CDS             complement(<1..322)
                     /locus_tag="PK818_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HQY83289.1"
                     /translation="MASPATPLVHPTAVIDPAARLGAGVQVGAYAVIGAEVEIGDDCR
                     IGPHCTIEGPTRIGRGNVVHAHAAIGGAPQDKKYRGERVELVIGDGNCIREFVTINRG
                     TGDGG"
     gene            complement(327..779)
                     /gene="fabZ"
                     /locus_tag="PK818_10970"
     CDS             complement(327..779)
                     /gene="fabZ"
                     /locus_tag="PK818_10970"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HQY83290.1"
                     /translation="MTVSLPATIADIQRLLPHRYPFLLVDRVVALEPHKRVLAYKHVT
                     ANEQFFQGHFPGRPVMPGVLVVEALAQAGGLLTQLSKQDGDDMEQLFYLVKVDNAKFS
                     RMVVPGDRLDLDVELKRQIRNMAQYVGVARVDGEQVACAEILCAAARA"
     gene            complement(776..1798)
                     /gene="lpxD"
                     /locus_tag="PK818_10975"
     CDS             complement(776..1798)
                     /gene="lpxD"
                     /locus_tag="PK818_10975"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007965614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HQY83291.1"
                     /translation="MPMPAFAASELALRFGLELRGEDRQVHGVGTLAMAGPDQLGFLA
                     NPRYRSQLADTAAGVVVLRAEDADARAGTLLVARDPYVAFARIASLFEPRPARVPGIH
                     PSAAIDPTATVDPAAQVGPHVAIGARSRIGAGACIGPGCVLGDDCEVGEGCELVARVT
                     LVTRVRLGKRVLVHPGAVLGADGFGIAMDHGHWTKVPQLGGVVVGDDCEIGANTTIDR
                     GALGDTVLEEDVRLDNQIQVGHNARIGAHTAMAGCSAVAGSASIGRHCLVGGGAGILG
                     HLEVCDRVVITAMSLVTHSIREPGEYSSGTPLMDNRNWRKSAARFKQLDRIARAIPGG
                     GDKDTA"
BASE COUNT          276 a          691 c          678 g          285 t
ORIGIN      
        1 cgccgccgtc gccggtgccg cggttgatgg tgacgaactc gcggatgcag ttgccgtcgc
       61 caatcaccag ttcgacccgc tcgccgcggt atttcttgtc ctgcggcgca ccaccgatcg
      121 cggcatgcgc gtgcacgacg ttgccacgcc cgatccgggt cgggccttcg atggtgcagt
      181 gcgggccgat gcggcagtcg tcgccgatct ccacctccgc gccgatcacc gcgtaggcgc
      241 cgacctgcac gcccgcgccg agccgcgccg ccgggtcgat cacggcggtc gggtggacca
      301 ggggcgtggc cgggctcgcc atcggatcag gcccgtgccg ccgcgcacag gatctcggcg
      361 caggcgacct gctcgccatc gacgcgggcg acgcccacgt attgcgccat gttgcggatc
      421 tgccgcttca gctcgacatc gaggtcgagg cggtcgcccg gcaccaccat ccggctgaac
      481 ttcgcgttgt cgaccttgac caggtagaac agctgctcca tgtcgtcgcc gtcctgcttg
      541 gagagctggg tgagcaggcc gccggcctgc gccaacgcct ccaccaccag caccccgggc
      601 atcaccgggc ggcccgggaa gtggccctgg aagaactgct cgttggcggt gacgtgcttg
      661 tacgccagca cccgcttgtg cggctccagc gcgaccaccc ggtccaccag caggaacggg
      721 tagcggtgcg gcagcaggcg ctggatgtcg gcgatcgtgg cgggcaatga aacggtcatg
      781 cggtgtcctt gtcgccgcca cccgggatgg cccgggcgat gcggtcgagt tgcttgaaac
      841 gcgcggcgct cttgcgccag ttgcgattgt ccatcagcgg ggtgcccgac gaatactcgc
      901 cgggttcgcg gatcgaatgg gtgaccagcg acatcgcggt gatgaccacc cggtcgcaga
      961 cctcgaggtg gccgaggatg ccggcgccgc cgccgaccag gcagtggcgg ccgatgctcg
     1021 cgctgccggc caccgccgaa caaccggcca tcgcggtgtg cgcgccgatc cgggcgttgt
     1081 ggccgacctg gatctggttg tccaggcgca cgtcctcttc cagcacggtg tcgccgagtg
     1141 caccgcgatc gatggtggtg ttggcgccga tctcgcaatc gtcgccgacc accaccccgc
     1201 cgagctgcgg caccttggtc cagtggccgt ggtccatggc gatgccgaag ccatccgcgc
     1261 ccagcaccgc gcccggatgc accagcaccc gcttgccgag ccggacccgg gtcaccaggg
     1321 tcacgcgggc aaccagttcg cagccttcgc cgacctcgca gtcgtcgccg agcacgcagc
     1381 cggggccgat gcaggcgccc gcgccgatcc ggctgcgtgc gccgatcgcg acatgcgggc
     1441 cgacctgcgc ggcgggatcg accgtggcgg tcggatcgat cgcagcgctg ggatggatgc
     1501 cgggcacgcg cgccggcctc ggttcgaaca gggaggcgat ccgggcgaac gcgacgtacg
     1561 gatcgcgcgc caccagcaac gtccccgcgc gcgcatccgc atcctccgcg cggagcacca
     1621 ccacgccggc ggcggtgtcc gccagctggc tgcggtagcg cgggttggcg aggaagccga
     1681 gctggtccgg gcccgccatc gccagggtac cgacgccgtg cacctggcgg tcttcgccgc
     1741 gcagttcaag cccgaaccga agcgccagtt ccgaggctgc gaaggctggc atgggcatgc
     1801 gcggtatccg cggttacagc tggccgccga aggtgaactg cagccgctcg atctggtcgt
     1861 agtccttctt gcgcagcggg aacgcgtagc tgatcgacag cggacccatc ggcgagcgcc
     1921 actgcagggc
//
LOCUS       DAORUK010000252         1883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_100248_length_1883_cov_19.823851, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000252 DAORUK010000000
VERSION     DAORUK010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100248_length_1883_cov_19.823851"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PK818_10980"
     CDS             <1..979
                     /locus_tag="PK818_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQY83292.1"
                     /translation="TDTPATPGDMPMPVRLHAVGRIALAALLGLCLPACERGTPGTGA
                     PIGVAVKPQAQAARKPVEAVNVLRDRLLARDGAGFARLAVPPDLHARLAAGWREGRTR
                     WPLDELPLDAKLPKMLAALQENDAEARLMATFGRQFAGADRDIDQAIRTLVQFGGEYV
                     QKQAGYTPEEREHVAQALAAVGGWALAAPLSDPKHARPFFAALVAAARRSGIEGKAGP
                     AAYATLGMDPSLNRLSPFIATLLAQLRSQYGLDLDASLRGMQVRLLEQTGDSARLRLR
                     YQLAGSEIDAVVPVQRIDGHWYLADFLRRAEASLAGRQAAAGAKNPASP"
     gene            979..>1883
                     /locus_tag="PK818_10985"
     CDS             979..>1883
                     /locus_tag="PK818_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase"
                     /protein_id="HQY83293.1"
                     /translation="MSAMPNRPDLPSQPSLFDAAPAAEPAVVATTTGSAPAQADSAAG
                     PARAAAEPAADSPQLPLPAFLADPPRPAPVRVTRPPLWARVLGWLIDPWLKLELEPNE
                     PGRHHAGQPVCYVLEDYGLSNALILERACREAGLPSPLQPMAGDPLGRKRAYVALSRR
                     HANALGLLPGPEHKTHSGSLARLLEAHRANPDLDVQLVPVSIFVGQAPNRSSGWFSVL
                     FSENWTIVGRFRRMLALLLNGRDTLVQFAPPVALRAIVGEGLPPERTVRKLSRVLRTH
                     FRRVREVVVGPDLSTRRMLVDKVLAA"
BASE COUNT          248 a          725 c          661 g          249 t
ORIGIN      
        1 caccgacact ccggcaacgc ctggagacat gccgatgccc gttcgcctgc acgccgttgg
       61 ccgcatcgcc ctggctgccc tcctcggcct gtgcctgccc gcctgtgaac gcggcacgcc
      121 cgggaccggc gcgccgatcg gggttgccgt gaaaccgcag gcacaggccg cccgcaagcc
      181 ggtggaggcg gtcaatgtcc tgcgcgaccg cctgctggcc cgcgatggcg ccggcttcgc
      241 gcggctggcg gtgccgccgg acctgcatgc acggctcgcc gccggctggc gcgaggggcg
      301 cacgcgctgg ccgctggacg aactcccgct ggatgcgaaa ttgccgaaga tgcttgcagc
      361 tttgcaggag aacgacgccg aagccaggtt gatggcgaca tttggccgcc aattcgccgg
      421 cgccgaccgc gacatcgacc aggcgatccg gaccctggtc cagttcggcg gcgaatacgt
      481 ccagaagcag gccggctaca cccccgagga gcgcgagcac gtggcccagg cgctggccgc
      541 cgtgggtggc tgggcgctgg cggcgccgct gtcggatccg aaacacgccc gcccattctt
      601 cgcggcgctg gtcgcggcgg cgcgccgcag cggcatcgaa ggcaaggccg gccccgccgc
      661 ctacgccacg ctgggcatgg accccagcct gaaccggctg tcgccgttca tcgccaccct
      721 gctggcgcag ctgcgcagcc agtacggcct cgacctcgac gccagcctgc gcgggatgca
      781 ggtgcgcctg ctcgagcaga ctggcgacag cgcccgcctg cggctgcgct accagctggc
      841 cgggagcgag atcgatgcag tcgtgccggt gcagcgcatc gatgggcact ggtacctggc
      901 cgatttcctg cgccgcgccg aggcctcgct ggccggcagg caagccgcgg cgggcgcgaa
      961 aaaccccgct tcgccataat gtcggcgatg cccaaccgtc ccgacctccc cagccagccc
     1021 agcctgttcg acgccgcgcc cgccgccgaa cccgccgtcg tggccacaac cacgggcagc
     1081 gcgcccgcgc aggcggactc cgcggccggc ccggcgcgcg ccgccgccga gccggcagcg
     1141 gacagcccgc agctgccgtt gccggcgttc ctggccgacc cgccgcggcc cgcgccggtg
     1201 cgcgtgaccc ggccgccgct gtgggcgcgg gtgctgggct ggctgatcga cccttggctc
     1261 aagctcgagc tcgaaccaaa tgaaccgggc cgccaccacg ccggccagcc ggtctgctac
     1321 gtgctggagg actacggcct gtccaacgcg ctgatcctgg agcgcgcctg ccgcgaggcc
     1381 ggcctgccgt caccgctgca gccgatggcc ggcgacccgc tgggccgcaa gcgcgcctac
     1441 gtcgcactgt cgcgccggca tgccaatgcg ctggggttgc tgcccggccc cgaacacaag
     1501 acccactcgg gttcgctggc gcggctgctg gaggcgcacc gcgccaaccc ggacctggac
     1561 gtgcaactgg tcccggtgtc gatcttcgtc ggccaggcgc ccaaccgcag cagcggctgg
     1621 ttcagcgtgc tgttctcgga gaactggacc atcgtcggcc gcttccgccg gatgctggcg
     1681 ctgctgctca acggccgcga caccctggtc cagttcgcgc cgccggtggc gctgcgcgcg
     1741 atcgtcggcg aggggctgcc gcccgaacgc acggtgcgca agctctcgcg cgtgctgcgc
     1801 acccacttcc gccgcgtgcg cgaggtggtg gtcggccccg acctgtcgac ccggcgcatg
     1861 ctggtggaca aggtgctggc cgc
//
LOCUS       DAORUK010000253         1838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_104533_length_1838_cov_19.383623, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000253 DAORUK010000000
VERSION     DAORUK010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104533_length_1838_cov_19.383623"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1838)
                     /gene="purL"
                     /locus_tag="PK818_10990"
                     /gene_synonym="purI"
     CDS             complement(<1..>1838)
                     /gene="purL"
                     /locus_tag="PK818_10990"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HQY83294.1"
                     /translation="GDAVIVLGGPAMLIGLGGGAASSVASGDTSEDLDFASVQRDNPE
                     MERRAQEVIDRCVALGDANPIQSIHDVGAGGLSNAIPELLHDSGVGGVIDLGRVPKDD
                     PALSPMQLWCNESQERYVLGIPPSRVAEFAALCARERCVFAVVGTATAEERLVVGYAG
                     EAAAIDLPMDVLFGKAPKMHRDTAHPAPPRWPEADTDALDLRDAGLRVLAHPTVAAKT
                     FLVTIGDRTVGGLTARDQMIGPWQVPVADCAITLSGFDGNTGEAMAIGERTPLALLDA
                     AASARMAVGEAITNLLAAPVESLNRVKLSANWMAAAGHPGEDARLFDAVKAVGLELCP
                     ELELSIPVGKDSLSMQAQWQADGSAFKSVSPVSLVVSAFAPVVDVRQQLTPLLRRDVE
                     SELWLVGLGAGKQRMGGSVLAQCHPGATSSAREDGAALPAFGGDVPDLDDPQRLRALF
                     ELVRDAREDGLLLAYHDRSDGGAFAALCEMAFASRMGLDIRLDGWGDGRSGDVFRTLF
                     NEELGAIVQVPVTERAAFADLVAQHGLVECTQRIATATGAPVIRVSDDDEILAEWRWD
                     ELFDAWWSVTHAMQRLRDNPDGADSERESARRFDAPGLQPKLTFDPA"
BASE COUNT          265 a          678 c          643 g          252 t
ORIGIN      
        1 gccgggtcga aggtgagttt cggttgcagg ccgggtgcgt cgaagcggcg cgcggactcg
       61 cgctcgctgt cggcgccgtc cgggttgtcg cgcaggcgct gcatcgcgtg ggtgaccgac
      121 caccaggcgt cgaacagttc gtcccagcgc cattcggcca ggatctcgtc gtcatcgctg
      181 acccggatca ccggcgcgcc ggtggcggtg gcgatccgct gggtgcattc gaccagcccg
      241 tgctgggcga ccaggtcggc gaacgccgcg cgttcggtaa ccggcacctg cacgatcgcc
      301 cccagttcct cgttgaacag ggtgcggaac acgtcgccgg agcggccatc gccccagccg
      361 tcgaggcgga tgtccaggcc catgcgcgag gcgaacgcca tctcgcacag ggcggcgaag
      421 gcaccgccgt cggaacggtc gtggtaggcc agcagcaggc cgtcctcgcg cgcgtcgcgg
      481 accagttcga acagcgcgcg caggcgctgc gggtcgtcga ggtcgggcac gtcgccgccg
      541 aacgcgggca gcgccgcgcc atcttccctc gcactggacg ttgcgcccgg atggcactgc
      601 gccagcaccg aaccacccat gcgctgcttg cccgcgccca ggccgaccag ccacagctcg
      661 gactccacgt cgcggcgcag cagcggggtg agctgctggc gcacgtcgac caccggcgcg
      721 aacgcggaca ccaccagcga caccggcgag accgacttga acgcgctgcc atcggcctgc
      781 cattgcgcct gcatcgacag cgaatccttg ccgaccggga tgctcaattc gagctccggg
      841 cacagttcca ggcccaccgc cttgaccgcg tcgaacaggc gcgcgtcctc gcccgggtgg
      901 ccggcggcgg ccatccagtt ggccgacagc ttgacccggt tcagcgattc caccggcgcg
      961 gcgagcaggt tggtgatcgc ttcgcccacc gccatccgcg ccgaggcagc ggcatccagc
     1021 aaggccagcg gagtgcgttc gccgatcgcc atcgcctcgc cggtgttgcc gtcgaaaccg
     1081 ctgagggtga tcgcgcagtc ggccaccggc acctgccacg ggccgatcat ctggtcgcgc
     1141 gcggtcagcc cgccaacggt gcggtcgccg atggtgacca ggaaggtctt ggcggccacg
     1201 gtcgggtgcg cgagcacgcg caggccggcg tcgcgcaggt cgagcgcgtc ggtatccgcc
     1261 tccggccagc gcggcggcgc cggatgcgcg gtgtcgcggt gcatcttcgg tgccttgccg
     1321 aacagcacgt ccatcggcag gtcgatcgcc gcggcctcgc ccgcgtagcc gaccaccagc
     1381 cgctcctccg cagtggcggt gccgaccacc gcgaacacgc agcgctcgcg cgcgcacagc
     1441 gcggcgaatt cggccacccg cgagggcggg atgccgagca cgtagcgctc ctgcgattcg
     1501 ttgcaccaca gttgcatcgg cgacagcgcg ggatcgtcct tcggcacccg gccgaggtcg
     1561 atcacgccgc cgacgccgga gtcgtgcagc agctccggga tcgcattgga caggccgccg
     1621 gcgccgacgt catggatcga ctggatcggg ttggcatcgc ccagggcgac gcaacggtcg
     1681 atcacttcct gcgcgcgccg ctccatctcc gggttgtcgc gctgcaccga ggcgaaatcg
     1741 aggtcttcgc tggtgtcgcc cgaggccacc gaactggcgg cgccgccgcc gaggccgatc
     1801 agcatcgccg ggccgccgag cacgatcacc gcgtcgcc
//
LOCUS       DAORUK010000254         1826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_105741_length_1826_cov_16.901186, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000254 DAORUK010000000
VERSION     DAORUK010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105741_length_1826_cov_16.901186"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /gene="pgk"
                     /locus_tag="PK818_10995"
     CDS             <1..269
                     /gene="pgk"
                     /locus_tag="PK818_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HQY83295.1"
                     /translation="AKAGTVVWNGPVGVFEFDAFGHGTEVVARAIAASPAFSIAGGGD
                     TLAAVDKYGIEADISYISTGGGAFLEFLEGKELPAVAALQARSG"
     gene            364..>1826
                     /gene="pyk"
                     /locus_tag="PK818_11000"
     CDS             364..>1826
                     /gene="pyk"
                     /locus_tag="PK818_11000"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HQY83296.1"
                     /translation="MAAHRRRTRIVATLGPATDPPEVLEAVLRAGVDVVRLNFSHGDP
                     SAQVARAEAVRAMAAQIGREVGILADLPGPKIRVETFAAGKVQLKAGNRFDLVASLSA
                     PPGDDTQVGVSYLELTGDVVPGDLLLLDDGIVQLQVVAVEGERIVTTVLNDCVLSNRK
                     GLNKQGGGLSLGAITEKDRELIAVAAGMQVDFIAVSFCRNAEDMHEARRVARAHGSNA
                     ALVAKVERAEAIENLTEIVDASDVVMVARGDLGVEIGDAELPGLQKKIIREAVAREKV
                     VITATQMLQSMVDSPIPTRAEVLDVANAVIDGTDAVMLSQESAAGSWPVKAVEAMARI
                     CVGAEKQFVADTDFEAARRGLERADQAIAMATMFLSEHIGVRAVVAMTESGGTARFLS
                     RFRSSVPIYAFSRHAPARRKMALMRDVFPMGYDSRGQTPREAARGSVRLLVDAGLLQA
                     GDRVVFTSGEHMETRGATNTLRLLEIGEDGRAEGLGEL"
BASE COUNT          276 a          593 c          687 g          270 t
ORIGIN      
        1 tcgcgaaggc cggcaccgtg gtctggaacg gcccggtcgg cgtgttcgag ttcgatgcat
       61 tcggccacgg caccgaagtg gtggcccgcg cgatcgcggc atcgccggcg ttctccatcg
      121 ccggtggtgg cgacaccctg gccgcggtgg acaagtacgg catcgaggcc gacatctcct
      181 acatctccac cggcggcggc gcgttcctgg agttcctgga aggcaaggaa ttgccggcgg
      241 tggcggcgct gcaggcgcgc tccggatgaa gtcgattgca atgtccggcg agacatcgcc
      301 ggtcgttgcg ctgtgctagc gtgcgggcaa ctggagcccg cacacatgac tgcaccacac
      361 gacatcgccg cccaccggcg acgtacccgc atcgtcgcca cccttggccc ggcgaccgat
      421 ccacccgagg tgctggaggc ggtgttgcgc gcgggggtgg acgtggtgcg gctgaacttc
      481 tcgcatggcg atccgtcggc gcaggtcgcg cgcgcggagg cggttcgcgc gatggcggcg
      541 cagatcggac gcgaggtcgg catcctcgcc gacctgccgg gcccgaagat ccgggtcgag
      601 accttcgccg ccggcaaggt gcagctgaag gccggcaacc gcttcgacct ggtcgccagc
      661 ctgtcggcgc cgcccggcga tgacacccag gtcggcgtga gctacctgga gctgaccggc
      721 gacgtggtcc ccggcgacct gttgctgctg gacgacggca tcgtccagtt gcaggtggtg
      781 gcggtggagg gcgagcgcat cgtcaccacc gtgctcaacg actgcgtgct gtccaaccgc
      841 aagggcctga acaagcaggg cggcgggctg tcgctgggtg cgatcaccga gaaggaccgc
      901 gagctgatcg cggtcgcggc cgggatgcag gtggacttca tcgcggtctc gttctgccgc
      961 aacgccgagg acatgcacga ggcgcgccgg gtggccaggg cgcacggcag caacgcggcg
     1021 ctggtggcga aggtcgaacg cgccgaggcg atcgagaacc tcaccgagat cgtcgatgcc
     1081 agcgacgtgg tgatggtcgc gcgcggcgac ctcggcgtgg agatcggcga cgccgaattg
     1141 ccgggcctgc agaagaagat catccgcgag gcggtggcgc gcgagaaggt ggtgatcacc
     1201 gcgacgcaga tgctgcagtc gatggtggac agcccgatcc cgacccgcgc cgaggtgctg
     1261 gacgtggcca acgcggtgat cgacggcacc gatgcggtga tgctgtcgca ggaatcggct
     1321 gccggcagct ggccggtgaa ggcggtcgag gcgatggcgc ggatctgcgt cggcgccgag
     1381 aagcagttcg tcgccgatac cgatttcgag gcggcgcggc gcggcctgga gcgcgccgac
     1441 caggccatcg cgatggccac catgttcctg tccgagcaca tcggcgtgcg cgcggtggtg
     1501 gcgatgaccg aatccggcgg caccgcgcgc ttcctctcgc gcttccgctc ctcggtgccg
     1561 atctacgcgt tctcccggca tgcgccggcg cgccgcaaga tggcgctgat gcgcgacgtg
     1621 ttcccgatgg gctacgacag ccgcggccag accccgcgcg aagccgcgcg cggcagcgtc
     1681 cgcttgttgg tggacgccgg tttattgcag gcgggcgacc gggtggtgtt caccagcggc
     1741 gagcacatgg aaacccgcgg tgccaccaac accctgcgcc tgctcgagat cggcgaggac
     1801 ggccgtgcgg aggggttggg cgagct
//
LOCUS       DAORUK010000255         1824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_105914_length_1824_cov_16.469192, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000255 DAORUK010000000
VERSION     DAORUK010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105914_length_1824_cov_16.469192"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PK818_11005"
     CDS             complement(<1..313)
                     /locus_tag="PK818_11005"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HQY83297.1"
                     /translation="MHADLHQDPRIECHVVDIAGGRLDPAAAIAFVSDDAHGGIDVFI
                     GRVRASSHGRQCVAIHYDMFDPLALTIFRSTAAAAVERFGPKAKCYVAHARGKLAVGD
                     LA"
     gene            complement(316..576)
                     /locus_tag="PK818_11010"
     CDS             complement(316..576)
                     /locus_tag="PK818_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HQY83298.1"
                     /translation="MKLTVQLFGRFREFSAAPEIALELPGIATVAEFRAAFDAWAGAH
                     WPGYTPGLLKASAIATESTLLHAGSALPADGRLALLPPVSGG"
     gene            complement(573..>1824)
                     /locus_tag="PK818_11015"
     CDS             complement(573..>1824)
                     /locus_tag="PK818_11015"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HQY83299.1"
                     /translation="MLSIDDALALIRDCCAPLGTEQVPLADAGGRVLAAPVDAPRDLP
                     PFANSAMDGFALRWHDGLAGATTLPVLAEQAAGDGIAGQSDGACSIMTGARIPDGLDT
                     VVPVEDCRVVARDGEGRPLVIALAHAPARGQHVRHAGEDVAAGERVLEAGTRLDEEAL
                     LVLRGIGIGTVAVHRRPRLALACTGRELVDTDGAALAPGQIANTNGPYLQRLFADAGA
                     EVVERVTLADEPDAFATWLARWLDAGIELVVTTGAVSMGRYDFVPDVLRAAGAELRFH
                     KLKMRPGKPLLFATAGRGTLVFGLPGNPVSSTVGARVFVDAALRALAGRAPEPALLLP
                     LAAAARKKPGFSMLQKAALALDADGRLQVRLLQGQESFKTAPLLAARAWAVLPEDAAE
                     LAAGTHVPVYPLRPGGAPFDGLTR"
BASE COUNT          248 a          666 c          683 g          227 t
ORIGIN      
        1 ccgcgaggtc gccgaccgcg agcttgccgc gggcatgggc gacgtagcac ttcgccttcg
       61 ggccgaaacg ctcgaccgcc gccgcggcgg tgctgcggaa gatcgtgagc gccagcggat
      121 cgaacatgtc gtagtggatg gcgacgcact ggcggccatg gctggacgcg cgcacgcggc
      181 cgatgaacac gtcgatgccg ccgtgggcgt cgtcgctgac gaacgcgatc gccgccgccg
      241 ggtccagccg gcccccggcg atgtcgacca cgtggcattc gatccgcgga tcctggtgca
      301 ggtcggcgtg catgctcagc ctccgctcac cggcggcagc agcgccagcc ggccatcggc
      361 gggcagcgcg ctgccggcgt gcagcagggt ggattcggtg gcgatcgccg aggccttgag
      421 caggcctggc gtatagcccg gccagtgcgc gcctgcccac gcgtcgaagg cggcgcggaa
      481 ttcggccacg gtggcgatgc cgggcagttc cagcgcgatc tcgggggccg cgctgaattc
      541 gcggaagcgg ccgaacagtt gcacggtcag tttcatcggg tcagtccatc gaacggggcg
      601 ccaccggggc gcagcggata caccggcacg tgcgtcccgg cggcaagctc ggcggcgtct
      661 tcgggcagca cggcccacgc gcgcgcggcc agcagcggcg ccgtcttgaa gctttcctgg
      721 ccctgcagca ggcgtacctg caggcggccg tccgcatcca gcgccagcgc ggccttctgc
      781 agcatcgaaa agccgggctt cttgcgcgcg gcggcggcca gcggcagcag cagcgccggt
      841 tccggcgcgc ggccggccag cgcgcgcagc gcggcatcga cgaacacgcg cgcacccacg
      901 gtgctcgaca ccgggttgcc cggcaggccg aacaccaggg tgccgcggcc ggcggtggcg
      961 aacagcagcg gcttgcccgg gcgcatcttc agcttgtgga agcgcagctc cgcgccggcc
     1021 gcgcgcagca cgtcggggac gaagtcgtag cggcccatcg agaccgcgcc ggtggtgacg
     1081 acgagctcga tcccggcgtc cagccagcgc gccagccatg tggcgaaggc gtcgggttcg
     1141 tcggccaggg tgacgcgctc gaccacctcg gcgccggcat ccgcgaacag gcgttgcagg
     1201 tacggaccat tggtgttggc gatctgcccg ggcgccagtg cggcgccgtc ggtgtccacc
     1261 agttcgcggc cggtgcaggc cagcgccagc cgcggccggc ggtgcacggc cacggtgccg
     1321 atgccgatcc cgcgcagcac cagcagggct tcctcgtcca gccgggtacc ggcctccagc
     1381 acgcgctcac cggcggccac gtcctcgccg gcgtggcgca cgtgctgccc acgcgcgggg
     1441 gcgtgcgcca gtgcgatcac caacggccgg ccttcgccgt cgcgcgcgac cacccggcag
     1501 tcctcgaccg gaaccacggt gtccagcccg tccgggatgc gcgcgccggt catgatgctg
     1561 caggcaccgt cggactggcc ggcgatgccg tcgccggcgg cctgttcggc cagcaccggc
     1621 agggtggtgg cgccggccag gccgtcgtgc cagcgcaggg cgaagccgtc catcgccgaa
     1681 ttggcgaatg gcggcaggtc gcgcggggca tccaccggcg ccgccagcac ccggccgccg
     1741 gcgtccgcca gcggtacctg ttcggtgccc agcggcgcgc agcaatcgcg gatcagcgcc
     1801 agcgcgtcgt cgatggagag catc
//
LOCUS       DAORUK010000256         1816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_106720_length_1816_cov_17.078365, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000256 DAORUK010000000
VERSION     DAORUK010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106720_length_1816_cov_17.078365"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..998)
                     /locus_tag="PK818_11020"
                     /pseudo
     CDS             complement(81..998)
                     /locus_tag="PK818_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357441.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            1116..1697
                     /locus_tag="PK818_11025"
     CDS             1116..1697
                     /locus_tag="PK818_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQY83300.1"
                     /translation="MKLLHLDSSILGPQSASRELSAAVVARWQREMPGLAVVHRDLAA
                     DPLPHLSGASLAQADADEAALAATVMDEFLQADVIVVGAPMYNFGIPSQLKAWIDRVA
                     VAGKTFRYTADGPQGLAGGKRVVVAASYGGFHAADASSNFVEPYLRFVFGFLGIDDIE
                     FVTVEGLAVSPEQRAAAMSDAHGRIDVAPPLAA"
BASE COUNT          283 a          661 c          569 g          303 t
ORIGIN      
        1 atctcaccgc ttccgccagt tcacgcttgc ctgggcgctt tgcgcttgag cttgcccttg
       61 tccttgcagc ccttctcgct gcactccagt cgcgccgctt ccatcaacgg tggcagcttc
      121 tcggccagga agtcgatcag cacccgcacc gccggcagca ggccgcggcg tgaggcgaac
      181 accgcatgcg cgatgccctg cggcaggcgc cattgcggca ataccacttc cagttcgcca
      241 cggcgcacgg catcggcgca gacggtttcg ggcagcaggg tgatgccgat gccatcgcgc
      301 gccatcgcct gcatcatcgg gaaatcgaag ccggtgacgc gcggctgcag ttcgatcctg
      361 cgcacttcgc cctccggccc gtgcagttcc cagcgctggc gggcatcgtc ctcgctgatg
      421 ctgagggtga cgtgctcgac cagcgcttcc ggcacctgcg gccggccggc gcggtccaga
      481 taccccgggc ttgccaccag caattcctgg atctggccga agctgcgcat caccaggctg
      541 ccgtcatcgt ccagtttgct gcgcacgcgc agggcgacat ccacgccttc gttgatgaca
      601 tcgacccgac gattgctgac gatcaactgc agccgcacct tcgggtacag cgccatgaat
      661 tccggcagca gcttggggaa ctgctgctgc gccatcgaca ccggcacgct gatccgcacc
      721 gtgccgcgcg gctccgcact gagccggtcc accgcctcgc gcgcggcctg cgcctcggcc
      781 agcatcgact gcgcgtggcg atgcacgctg gtgccgacgt cggtgaccgc gaaacgccgg
      841 gtcgaccgct gcagcaggcg caccccgagt tcgctttcca gcgcgctgat gcgccggctc
      901 aggcgcgatt tcgggatccc cagcgcccgt tccgcggcgg cgaacccgcc gtggtcgacg
      961 actgctgcga agtagtacag gtcgttcagg tcttgcatgt cgataattcc atgtatggaa
     1021 cgtatcgtga tattgaaacc tgtttatccc ggatatgcaa cagcgcacga tgcaccccga
     1081 cgcggcacat cgccgcccgc agacggggac cacccatgaa actcctccac ctcgattcca
     1141 gcatcctcgg cccgcagtcg gccagccgcg aactcagtgc ggcggtggtg gcccgctggc
     1201 agcgcgaaat gcccggcctg gcagtcgtcc atcgcgatct cgccgcggat ccgctgccgc
     1261 acctgagcgg cgcctcgctg gcccaggccg atgccgacga agccgcgctc gccgccaccg
     1321 tgatggacga attcctgcag gccgacgtga tcgtggtcgg cgcgccgatg tacaacttcg
     1381 gcatccccag ccagctgaag gcgtggatcg accgggtggc agtggccggc aagaccttcc
     1441 gctacaccgc cgacggcccg cagggcctgg ctggcggcaa gcgggtggtc gtcgccgcca
     1501 gctatggcgg cttccatgcc gccgatgcca gcagcaactt cgtcgaaccc tacctgcgct
     1561 tcgtgttcgg cttccttggc atcgacgaca tcgaattcgt caccgtcgaa ggcctcgcgg
     1621 tgtcgccgga acagcgggca gccgcgatgt ccgatgcgca cggcaggatc gacgtcgcgc
     1681 cgccactggc ggcctgaacc aggtttcgcg ttcccagccc ctcccctgcg caggaacgcg
     1741 acgggcccgc actgcgggcc ctttttttgc agcgaggccg cgcagcaagc cgcgcgcgaa
     1801 agacgccgcg tgcgga
//
LOCUS       DAORUK010000257         1784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_109956_length_1784_cov_17.123771, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000257 DAORUK010000000
VERSION     DAORUK010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109956_length_1784_cov_17.123771"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PK818_11030"
     CDS             complement(<1..481)
                     /locus_tag="PK818_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQY83301.1"
                     /translation="MSAASTRMSTAAYRIVENARLDARNTFGVQATAPLLVEVADASA
                     LPDLFGKAMPDDCPALVLGGGSNLLFAGDPAGVVLALTAQRIGIRDDDGARAIVRADA
                     GVGWHDLVLWTLGHGLCGLENLALIPGTVGAAPIQNIGAYGVEVCDRIHAVEAFDRPA
                     "
     gene            complement(478..1533)
                     /locus_tag="PK818_11035"
     CDS             complement(478..1533)
                     /locus_tag="PK818_11035"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HQY83302.1"
                     /translation="MYGLARPFLFAFDAERAHGLGLAALEAAYRSGLNPLLATVPKPL
                     PTKAFGLTFQNPLGLAAGMDKNGAHIDALMALGFGFVEIGTTTPRAQPGNPKPRMFRL
                     PEHEAVINRLGFNNEGVDALVRNVERAQRSRALLGINIGKNKDTPNESAESDYLFCLE
                     RVYPLADYITVNISSPNTAGLRELQEEQALRRLVGNLREAQEKLGAQHGKRVPMLVKI
                     APDLSESDIDAAGRVLGDLQVDGVIAGNTTISREGVEGARFANEVGGLSGAPLMGKST
                     VVLRMLRTRLPEAIPLVGVGGILHGADAATKQAAGATLMQVYTGLVYRGPALVGECVE
                     ALRRRKEAPSRGTLPPE"
     gene            complement(1552..>1784)
                     /locus_tag="PK818_11040"
     CDS             complement(1552..>1784)
                     /locus_tag="PK818_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQY83303.1"
                     /translation="KRAEQFLSAWGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRES
                     GSDAWKAYMRRQTNTINYSDSLPLAQGIKFDL"
BASE COUNT          241 a          639 c          619 g          285 t
ORIGIN      
        1 cggctgggcg gtcgaacgcc tccaccgcgt ggatgcgatc gcatacctcc acgccatagg
       61 cgccgatgtt ctggatcggc gcggcgccga ccgtgcccgg gatcagcgcc aggttctcga
      121 ggccgcacag gccatggccc agcgtccaca gcaccaggtc gtgccagccg acgccggcat
      181 cggcgcgcac gatcgcgcgg gcgccatcat cgtcgcggat tccgatccgc tgcgcggtca
      241 gcgccagcac cacgcccgcc ggatcgccgg cgaacagcag gttgctgccg ccgcccagca
      301 ccagcgccgg gcaatcatcc ggcatcgcct tgccgaacag gtccggcagt gcgctggcat
      361 ccgccacttc gaccagcagc ggcgcggttg cctggacgcc gaaggtattg cgcgcatcca
      421 gccgcgcgtt ctcgacgatg cggtacgccg cggtcgacat gcgggtcgac gcggcgctca
      481 ttcgggcggc agggtcccgc ggctgggcgc ttccttgcgc cggcgcaacg cctccacgca
      541 ttcgccgacc agggccgggc cgcgatagac caggccggtg tagacctgca tcagggtcgc
      601 gcccgcggcc tgcttggtgg cggcgtcggc gccgtgcagg atgccgccga cgccgaccag
      661 cgggatcgcc tccggcagcc gcgtgcgcag catccgcagc accaccgtcg acttgcccat
      721 cagcggcgcg ccggacaggc cgcccacttc attggcgaag cgcgcgccct ccacgccttc
      781 gcgcgagatc gtggtgttgc cggcgatcac gccgtccacc tgcaggtcgc cgagcacgcg
      841 cccggccgcg tcgatgtcgc tctcgctgag gtcgggcgcg atcttgacca gcatcggcac
      901 ccgcttgccg tgctgcgcgc cgagcttctc ctgcgcctcg cgcaggttgc cgaccagccg
      961 ccgcagcgcc tgttcctcct gcagttcgcg caggccggcg gtgttcggcg aggaaatgtt
     1021 gacggtgatg tagtcggcca gcgggtatac ccgctccagg cagaacaggt agtcggattc
     1081 cgccgattcg ttcggggtgt ccttgttctt gccgatgttg atgccgagca gtgcgcgcga
     1141 gcgttgcgcg cgctcgacgt tgcgcaccag cgcgtccacg ccctcgttgt tgaagccgag
     1201 gcggttgatc accgcctcgt gttccggcag ccggaacatc cgcggcttcg ggttgcctgg
     1261 ctgcgcgcgc ggggtggtgg tgccgatctc gacgaagccg aagcccagtg ccatcagcgc
     1321 gtcgatgtgg gcgccgttct tgtccatccc ggcggcaagg cccagcgggt tctggaaggt
     1381 cagcccgaac gccttggtcg gcagcggctt cggcacggtc gccagcagcg ggttcagccc
     1441 gctgcggtag gcggcctcga gcgcggccag gccaagcccg tgcgcgcgct cggcatcgaa
     1501 ggcgaacagg aaggggcggg cgagtccgta catccgtttg cgcggccggc gttacaggtc
     1561 gaacttgatg ccctgcgcca gcggcaggga atcggagtag ttgatggtgt tggtctgccg
     1621 gcgcatgtag gccttccagg catcgctgcc ggattcgcgg ccgccgccgg tctccttctc
     1681 gccaccgaac gcaccgccga tctccgcacc gctggtgccg atgttgacgt tggcgatgcc
     1741 gcagtccgag ccccacgcgg acaggaactg ctccgcgcgc ttga
//
LOCUS       DAORUK010000258         1754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_113234_length_1754_cov_28.315480, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000258 DAORUK010000000
VERSION     DAORUK010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113234_length_1754_cov_28.315480"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="PK818_11045"
     CDS             <1..1236
                     /locus_tag="PK818_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014509241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HQY83304.1"
                     /translation="YQRSILVGAIIMAAGLFMISMPDESVFKLGLATIIAGNGLFKPN
                     ISTMVGKLYSPTDERRDSGFTLFYMGINLGAMLAPILTGFLAERMLGGSSDSPAYKVV
                     FIASGVGMLVSLVWFWFGRAQLKGVGRPPEGAEGGGRVLMTAIGALVAIPVIYFLLSM
                     GASSLQWVLTAMFVGLGVMLLVEGIREGSVARDKVIALLIIFAFNVLFWMFFEQAGSS
                     FSFLSDKIVDRDLGAAGGFIWPNAWFQSVNSVAIITLAPVLAWLWVKMGRNNPSIPRK
                     FGLGIIFNGLAFLLLMFALSSLLDPQTMKIPFWTLFMVYVIQSIGELCLSPIGLSMTT
                     KLAPSRLVGFAMGGWFLSTGIGNNLSGIFASHVSGSEGMSVASAQSGYTFGFWILLGF
                     GVLLFLIAPLIQRLMHGVK"
     gene            complement(1305..>1754)
                     /locus_tag="PK818_11050"
     CDS             complement(1305..>1754)
                     /locus_tag="PK818_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HQY83305.1"
                     /translation="FLPYRLSVLSNRISSAIAREYSQRFALGVTEWRVMAVLARYPGL
                     SASKVALRTAMDKVAVSRAVARLLEAGRIEREFDDDDRRRSVLRLSAAGRAVHDQVAP
                     LALAFERHVLGGMPADERDLLFRLLDRLDELELRAEHAVSEPGTTQA"
BASE COUNT          231 a          598 c          591 g          334 t
ORIGIN      
        1 taccagcgct cgatcctggt cggcgcgatc atcatggccg ccggcctgtt catgatctcg
       61 atgccggacg agagcgtgtt caagctcggc ctggccacca tcatcgccgg caacggcctg
      121 ttcaagccga acatctcgac catggtcggc aagctgtatt cgcccaccga cgagcgccgt
      181 gattccggct tcaccctgtt ctacatgggc atcaacctcg gcgcgatgct ggcgccgatc
      241 ctgaccggct tcctcgccga gcgcatgctg ggcggctcgt ccgacagccc ggcctacaag
      301 gtggtgttca tcgcctccgg cgtcggcatg ctggtctcgc tggtgtggtt ctggttcggc
      361 cgggcccagc tgaagggcgt gggccgtccg ccggagggcg ccgagggtgg cggccgggtg
      421 ctgatgaccg cgatcggcgc gctggtggcg atcccggtga tctacttcct gctgtcgatg
      481 ggcgccagtt cgctgcagtg ggtgctcacc gcgatgttcg tggggctggg cgtcatgctg
      541 ctggtcgagg gcatccgcga gggctcggtg gcgcgcgaca aggtgatcgc gttgctgatc
      601 atcttcgcct tcaacgtgct gttctggatg ttcttcgagc aggccggcag ttccttcagc
      661 ttcctgtccg acaagatcgt ggaccgcgac ctcggcgccg ccggcggctt catctggccg
      721 aacgcctggt tccagtcggt gaactcggtt gcgatcatca ccctggcccc ggtgctggcc
      781 tggctgtggg tgaagatggg ccgcaacaac ccgtcgatcc cgcgcaagtt cggccttggc
      841 atcatcttca acggcctggc gttcctgctg ctgatgttcg cgctgtccag cctgctggac
      901 ccgcagacca tgaagatccc gttctggacg ctgttcatgg tctacgtgat ccagtcgatc
      961 ggcgagctgt gcctgtcgcc gatcggcctg tcgatgacca ccaagctggc gccgagcagg
     1021 ctggtgggct tcgcgatggg cggctggttc ctgtccaccg gcatcggcaa caacctgtcc
     1081 ggcatcttcg ccagccacgt gtccggcagc gaggggatgt ccgtggcctc cgcgcagtcc
     1141 ggctacacct tcggcttctg gatcctgctc ggcttcggcg tgctgctgtt cctgatcgcc
     1201 ccgctgatcc agcgcctgat gcacggcgtg aaatagttcg ccttgcggtg atgcggaaac
     1261 ggcggccgag gccgccgttt ccgttggtgc gcgacgggtt cggctcaggc ctgggtggtg
     1321 cccggctcgg aaacggcgtg ttccgcgcgc agttccagtt cgtccagccg gtccagcagg
     1381 cggaacagca ggtcgcgctc gtccgccggc atgccgccca gcacgtggcg ctcgaacgcc
     1441 agcgccagcg gcgcgacctg gtcgtggacc gcccgcccgg ccgcggacag ccgcagcacc
     1501 gagcgccggc ggtcgtcgtc gtcgaattcg cgctcgatcc ggcccgcctc cagcagccgg
     1561 gccaccgcgc ggctgaccgc gaccttgtcc atcgcggtgc gcagcgcgac cttgctggcg
     1621 gacaggccgg ggtagcgggc cagcaccgcc atcacccgcc attcggtcac ccccagcgcg
     1681 aaccgctgcg agtactcgcg ggcgatcgcg ctgctgatcc ggttggacag cacgctcagc
     1741 cggtacggca ggaa
//
LOCUS       DAORUK010000259         1752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_113436_length_1752_cov_18.624632, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000259 DAORUK010000000
VERSION     DAORUK010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113436_length_1752_cov_18.624632"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /gene="purF"
                     /locus_tag="PK818_11055"
     CDS             <1..1157
                     /gene="purF"
                     /locus_tag="PK818_11055"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HQY83306.1"
                     /translation="NTDALRREVFEQDRRNVNTESDSEVLLNVFAHELDSQGVLTPDA
                     VFRALEGVNRRAKGGYAVVCTVLGLGLVAFRDPNGIRPLVLGKRTANGIDEYIVASES
                     VALDVLGFERLRDVAPGEGIVITARGELFAQQCAPPLAHAPCIFEFVYFARPDSMIEE
                     ISVHKARMRMGVTLGEKILRERPGHDIDVVIPIPDTSRDAALEIASVLGVKYREGFIK
                     NRYVGRTFIMPGQGERAKSVKRKLNPIPLEFRNRVVLLVDDSIVRGTTSQQIVQMARD
                     AGARKVYLASAAPPVRYPNIYGIDMPSSEELVAHDRSEREIEQLLGCDWLVYQDLADL
                     EAAVAGPKFPGRKFDSSCFSGEYVTGIEAGYFERLQQLRSDEAKKKRRAS"
     gene            1339..>1752
                     /locus_tag="PK818_11060"
     CDS             1339..>1752
                     /locus_tag="PK818_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HQY83307.1"
                     /translation="MKSLLAAAHTCLAAASPDDKPARTFEAAAAFARGELSSDGELDG
                     PEPIRMPGRPATPKLVHPRDLPKRGFGSIEGRAAFIHAVAHIEFNAIDLAWDAVYRFR
                     GMPAQYYADWVSVANDEARHFTMLRARLRELGYDYG"
BASE COUNT          294 a          576 c          606 g          276 t
ORIGIN      
        1 tcaacaccga cgcgctgcgg cgcgaggtgt tcgagcagga ccgccgcaac gtcaacaccg
       61 agtccgattc ggaagtcctg ctcaacgtgt tcgcgcacga gctggacagc cagggcgtgt
      121 tgacgcccga cgccgtgttc cgcgcgctgg aaggcgtgaa ccgccgcgcc aagggcggct
      181 atgcggtggt ctgcacggtg ctcgggctgg gactggtcgc gttccgcgac ccgaacggga
      241 tccggccgct ggtgctgggc aagcgcaccg cgaacggcat cgacgagtac atcgtcgcct
      301 ccgaatcggt ggcgctggac gtgctgggct tcgagcgcct gcgcgacgtg gcgccgggcg
      361 agggcatcgt gatcaccgcg cgcggcgagc tgttcgcgca gcagtgcgcg ccgccgctgg
      421 cgcacgcgcc gtgcatcttc gaattcgtct atttcgcgcg gccggactcg atgatcgagg
      481 agatctcggt gcacaaggcg cgcatgcgga tgggcgtgac cctgggcgag aagatcctgc
      541 gcgagcggcc cggccacgac atcgacgtgg tgatcccgat cccggacacc agccgcgacg
      601 cggcgctgga aatcgccagc gtgctcgggg tgaagtaccg cgagggcttc atcaagaacc
      661 gctacgtcgg ccgcaccttc atcatgccgg ggcagggcga gcgggcgaag tcggtgaagc
      721 gcaagctcaa cccgatcccg ctggaattcc gcaaccgggt ggtgctgctg gtggacgatt
      781 ccatcgtgcg cggcaccacc agccagcaga tcgtgcagat ggcgcgtgac gccggtgcgc
      841 gcaaggtcta cctggcctcg gcagcgccgc cggtgcgcta tccgaacatc tacggcatcg
      901 acatgccctc gtccgaggaa ctggtggcgc acgaccgcag tgagcgcgag atcgagcaac
      961 tgctgggctg cgactggctg gtctaccagg acctggcgga cctcgaggcc gcggtggccg
     1021 gcccgaagtt cccgggacgc aagttcgaca gctcctgctt cagcggcgaa tacgtgactg
     1081 gcatcgaggc cggctatttc gagcgcctgc agcagctgcg gtcggatgag gcgaagaaga
     1141 agcgcagggc aagctgacgg tggctgatcg atggacgaat gccggggtgc gtttcgattc
     1201 gaccccggcc catcggcatg gccgatgggc gttcgtcagg ccgcagaccg gtggtctgca
     1261 agcgtccttc ctcacccccg atactggccc ggtcgaaacg caccccggca ttcgtcctgt
     1321 caagttctcg cggctcgaat gaagagcctt cttgcggctg cccacacctg cctcgccgcc
     1381 gcatcgcccg acgacaagcc cgcacggacg ttcgaagcgg cggctgcgtt tgcacgcggt
     1441 gagctgtcat ccgatggcga gctcgatgga ccggaaccga tccgcatgcc cggccggccg
     1501 gcaaccccga aactggtaca tccacgcgac ctgccgaaac gcgggttcgg cagcatcgag
     1561 ggccgcgccg ccttcatcca tgcggtcgcg catatcgagt tcaacgcgat cgacctggcc
     1621 tgggatgcgg tctatcgctt ccgcggcatg ccggcccagt actacgcgga ctgggtatcg
     1681 gtggcgaacg acgaggcgcg gcatttcacg atgctgcgcg cacggctgcg ggaactcggt
     1741 tacgactacg gc
//
LOCUS       DAORUK010000260         1739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_114924_length_1739_cov_19.847981, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000260 DAORUK010000000
VERSION     DAORUK010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114924_length_1739_cov_19.847981"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /gene="murD"
                     /locus_tag="PK818_11065"
     CDS             <1..435
                     /gene="murD"
                     /locus_tag="PK818_11065"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916415.1"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HQY83308.1"
                     /translation="LYVNDSISTTPHATLAALEVYRGRPVAVLVGGHDRGVPWDDFAA
                     AMRTQAPRAIVTMGQNGPRIHALLAPVAAASGFALQAASDLADAMDRARASLPAGGVV
                     LLSPGAPSFGAYKDYVARGRHFAQLAGFDPDAISSIGGMGIA"
     gene            519..1316
                     /locus_tag="PK818_11070"
     CDS             519..1316
                     /locus_tag="PK818_11070"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQY83309.1"
                     /translation="MRRALLAIGLLLATAPALAKMQAKPVEWSVGEDRFSGYVVYDDA
                     GKAQRPGLVMVPNWMGVTEDSLARASAVAGDDYVVLVADVYGAGVRPKNAGEAGKLAG
                     GLRGEDRATLRARIEGAVAALKAQAGAAPLDAGRIGAFGFCFGGSTVLELARGGTGLA
                     GVVSLHGGLAPGAKSTTATTVKTPVLVLNGAADKGVSDADIAAFEKEMDAAGADWQFV
                     DFAGAVHCFAEPGAGNDPASNCRYDERAARRAYRMMDDFFRERFAAE"
     gene            complement(1332..>1739)
                     /locus_tag="PK818_11075"
     CDS             complement(1332..>1739)
                     /locus_tag="PK818_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQY83310.1"
                     /translation="ADAATCDALHRYGMALGYAFQIADDVLDYASDTETLGKNLGDDL
                     AEGKATLPLIHAMQRSDAATRALLRGAIEQGDAGALPQVVAAIQACGSLGYSRDCAQR
                     YARDAAAALEGLDDNDFVAALRGLAQYSVNRDH"
BASE COUNT          241 a          592 c          640 g          266 t
ORIGIN      
        1 ctgtacgtca acgattcgat cagcaccacc ccgcacgcca ccctggcggc gctggaggtg
       61 tatcgcggtc ggccggtggc ggtcctggtg ggcggccacg atcgcggcgt gccgtgggat
      121 gacttcgccg cggcgatgcg cacgcaggca ccgcgcgcca tcgtcaccat gggccagaac
      181 ggcccgcgca tccatgcgct gctggcgccg gtcgccgcgg catccggttt cgcactgcag
      241 gccgcgagcg atctcgccga tgcgatggac agggcgcgcg catccctgcc tgctggtggg
      301 gtggtcctgc tgtcgccggg cgcgcccagc ttcggcgcct acaaggacta cgtggcgcgt
      361 ggccgccatt tcgcgcagct ggccggcttc gacccggacg cgatttccag catcggcggg
      421 atgggcatcg cctgaccgcg cccgccgcgt tcacgcgcgt tgaacctgcg ccgggtagcg
      481 taggcgccct caagaacgca cttcacaagg acccgcccat gcgccgcgca ctgcttgcca
      541 tcggattgct gctcgccacc gccccggccc tcgcgaagat gcaggccaag ccggtcgaat
      601 ggtcggtcgg cgaggatcgt ttcagcggct acgtcgtcta tgacgatgcc ggcaaggcgc
      661 agcgcccggg gctggtgatg gtgccgaact ggatgggcgt gaccgaggat tcgctcgcgc
      721 gcgcaagtgc cgtggccggc gacgactacg tggtgctggt ggcggacgtg tatggcgccg
      781 gcgtgcggcc gaagaatgcg ggcgaggccg gcaagctggc cggcggcctg cgcggcgagg
      841 atcgcgcaac gctgcgtgcg cgcatcgagg gcgcggtggc ggcgctgaag gcgcaggccg
      901 gtgcggcgcc actggatgcg gggcggatcg gcgcattcgg cttctgcttc ggcggatcca
      961 ccgtgctcga gctggcgcgc ggcggcaccg ggttggccgg cgtggtcagc ctgcacggcg
     1021 ggctggcgcc tggcgcgaaa tcaaccacgg ccacgaccgt gaagaccccg gtgctggtcc
     1081 tcaacggtgc cgccgacaag ggggtcagcg atgccgacat cgctgcattc gagaaggaga
     1141 tggatgccgc cggcgcggac tggcaattcg tcgatttcgc cggtgccgtg cattgcttcg
     1201 cggaacccgg cgccggtaac gacccggcca gcaactgccg ctacgacgag cgcgccgcca
     1261 ggcgcgccta ccggatgatg gacgacttct tccgtgaacg cttcgccgcc gaatgaacgc
     1321 ggcgtcgccg ctcagtggtc gcggttgacc gagtattgcg cgaggccgcg caatgcggcg
     1381 acgaaatcgt tgtcgtcgag cccctcgagc gcggccgcgg catcgcgcgc gtaccgttgc
     1441 gcgcagtcgc ggctgtagcc caggctgccg caggcctgga tcgcggccac cacctgcggc
     1501 agcgcaccag cgtcaccctg ttcgatcgcg ccgcgcagca acgcccgggt ggcggcgtcg
     1561 ctgcgctgca tggcatggat cagcggcagg gtcgccttgc cttcggcaag atcgtcgccg
     1621 aggttcttgc cgagcgtctc cgtgtcggag gcgtagtcca gcacgtcgtc ggcgatctgg
     1681 aaggcgtagc ccagcgccat cccgtagcga tgcaatgcgt cgcaggtggc cgcgtcggc
//
LOCUS       DAORUK010000261         1707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_118686_length_1707_cov_15.429177, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000261 DAORUK010000000
VERSION     DAORUK010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118686_length_1707_cov_15.429177"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="PK818_11080"
     CDS             complement(<1..1196)
                     /locus_tag="PK818_11080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY83311.1"
                     /translation="MGSIPAERASIRSPRKSHSLACLPLGADLALDPPPSCAWDFQSG
                     REAAPDVSSHRGVVREQLMPPWLSALFTRRMLICVFTGFSSGLPLYLLLNLVPAWLRS
                     EGVDLKTIGLFALIQFPYTWKFLWSPLLDRYAPPLGRRRGWMLITQIGLLASIAALGL
                     FAPASEIVPILWLTAAVALLSATQDIALDAYRREILSESELGLGNSVHVNAYRIAGLV
                     PGSLSLILADRLPWLQVFVITALFMLPGMLLALFAREPDVAGNAPKTLRDAVVEPFHE
                     FINRAGLREALLILAFIFLYKLGDSMCTALATPFYLDMGFAKSEIGIVAKNAGLWPAV
                     IGGLIGGLWMVKLGINRALWLFGVVQLVSIFGFAWLAWIGRHETVGALELTQLALVIG
                     FEALGVG"
     gene            complement(1169..1244)
                     /locus_tag="PK818_11085"
     tRNA            complement(1169..1244)
                     /locus_tag="PK818_11085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1209..1211),aa:Arg,seq:ccg)
     gene            1349..>1707
                     /locus_tag="PK818_11090"
     CDS             1349..>1707
                     /locus_tag="PK818_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQY83312.1"
                     /translation="MVHLNIRTFAAAGALALSVSVFAMGDRPRGDEEAANKRIAPVAR
                     VELAAPVAAASGNRSGEQIYKAVCGACHETGVAGAPKTGDKAAWAPRIALGLDGLLKS
                     AIAGKNAMPPKGGSDAN"
BASE COUNT          341 a          555 c          585 g          226 t
ORIGIN      
        1 ccgacgccga gcgcctcgaa accgatcacc agcgccagct gcgtcagttc cagcgcgccc
       61 accgtctcgt ggcggccgat ccaggccagc caggcgaagc cgaagatcga caccagctgc
      121 accacgccga acagccacag cgcgcggttg atgccgagct tcaccatcca caggccgccg
      181 atcaggccgc cgatcaccgc cggccacagc cccgcattct tcgcgacgat gccgatctcg
      241 gacttggcga aacccatgtc caggtagaag ggcgtggcca gcgccgtgca catcgaatcg
      301 cccagcttgt agaggaagat gaaggccagg atcagcagcg cctccctcag cccggcacgg
      361 ttgatgaact cgtggaaggg ttcgaccacg gcgtcgcgca aggtcttcgg tgcgttgccg
      421 gcgacatccg gctcgcgcgc gaagagcgcc agcagcatgc ccggcagcat gaacagcgcg
      481 gtaatgacga agacctgcag ccagggcagg cggtcggcca ggatcagcga gagcgaaccc
      541 ggcaccagcc cggcgatgcg ataggcattg acatgcaccg agttgcccag gcccagctcg
      601 gactccgaca ggatctcgcg ccggtaggca tcgagcgcga tgtcctgcgt cgccgagagc
      661 agcgccaccg cggcggtgag ccagaggatg ggcacgatct cggacgccgg cgcgaacagc
      721 ccgagcgcgg cgatgctggc cagcaggccg atctgggtga tcagcatcca gccgcggcgc
      781 ctgcccagcg gcggcgcata gcgatcgagc agcggggacc agaggaactt ccaggtgtag
      841 ggaaactgga tcagcgcgaa gaggccgatg gtcttgaggt ccacgccctc cgaacgcagc
      901 caggccggca ccaggttgag cagcaggtat agcggcaacc ccgacgaaaa gccggtgaac
      961 acacagatca gcatgcgccg ggtgaacagc gcggaaagcc agggcggcat cagctgttca
     1021 cggactacgc cgcggtggct ggaaacatcg ggcgcagctt cccggcccga ttgaaaatcc
     1081 caggcacaag acgggggcgg atcaagcgca aggtcggcac cgagaggcag acaagccagt
     1141 gaatgggact tcctggggga acgaatgctg gcgcgctcgg cggggatcga acccacaacc
     1201 cctggcttcg gaggccagta ctctatccat tgagctacga gcgcgcgagg gggcgaagca
     1261 tagcgcgttc ggccggtggc gtccattgcg gggcgggcga atccgctata atccggccct
     1321 ctgtacgact accccgggaa ggctgctcat ggtccatttg aatatccgaa cattcgccgc
     1381 ggcgggtgcg ctggctctct ccgtttcggt gtttgccatg ggcgaccgac ccaggggcga
     1441 cgaggaagcc gccaacaagc ggatcgctcc cgtggctcgc gtcgagcttg cggccccggt
     1501 ggctgcggct agcggcaatc gcagcggcga acagatttac aaggccgtct gcggcgcctg
     1561 tcatgagacc ggtgtcgccg gcgcgccgaa gaccggcgac aaggcggcct gggcgccgcg
     1621 catcgcgctg ggtctcgatg gcctgctgaa gtcggcgatt gccggcaaga acgcgatgcc
     1681 gccgaagggt ggctcggatg ccaacga
//
LOCUS       DAORUK010000262         1702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_119260_length_1702_cov_19.227080, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000262 DAORUK010000000
VERSION     DAORUK010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119260_length_1702_cov_19.227080"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PK818_11095"
     CDS             complement(<1..311)
                     /locus_tag="PK818_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQY83313.1"
                     /translation="MIEIDAASGPQPLGMSPAKFLREYWQKKPLLIRNAFPGFVSPVQ
                     PEDLAGLACEDGVLARLIEHDKADDSWRVRHGPFAETDFTTLPDHDWTLLVQDMDKWD
                     A"
     gene            complement(308..1114)
                     /gene="bla"
                     /locus_tag="PK818_11100"
     CDS             complement(308..1114)
                     /gene="bla"
                     /locus_tag="PK818_11100"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HQY83314.1"
                     /translation="MVAPCKDEGDWDEATAPRHVFANTWYVGSCGISAILITSPAGHI
                     LIDGATEAAAGGVARSIEASGHRMRDVRVILNTHEHFDHAGGIARLQQLSGAQVLSRA
                     AALPVLRSGRSERNDPQHLELKPFPAVPGARAIADDAHVQVGPLSVQVHATPGHAPGS
                     TSFSWRSCAGTDCRQVVYADSVSALADGEYLYRDHPDYGTAFKAGMQALAGIEPCDIL
                     LTPHPSVSDLWPRLRGEQPMGSAGACRALATTGLQNLQKRLDREAKEAPP"
     gene            complement(1218..>1702)
                     /locus_tag="PK818_11105"
     CDS             complement(1218..>1702)
                     /locus_tag="PK818_11105"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156796.1"
                     /note="Catalyzes two discrete reactions in the de novo
                     synthesis of purines: the cleavage of adenylosuccinate and
                     succinylaminoimidazole carboxamide ribotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HQY83315.1"
                     /translation="STMPHKVNPIDFENAEGNFGIASALFEHFAIKLPVSRWQRDLTD
                     STVLRALGTAFGHMLIGFDALLRGLDKLSVNPDRLAADLDAAWEVLAEAVQTVMRRHG
                     LPNPYEQLKALTRGHGITEASMREFIAGLDLPAGDRQRLLAMTPGSYTGLAERLAREI
                     "
BASE COUNT          252 a          579 c          594 g          277 t
ORIGIN      
        1 tcggcgtccc acttgtccat gtcctgcacc agcagggtcc agtcgtggtc gggcagggtg
       61 gtgaaatcgg tctccgcgaa cggcccgtgg cgcacgcgcc atgaatcgtc ggccttgtcg
      121 tgttcgatga gcctggccag cacgccatcc tcgcaggcca gcccggccag gtcttccggc
      181 tgcaccggcg acacgaagcc ggggaaggca ttgcggatca gcaggggttt cttctgccag
      241 tactcgcgca ggaacttcgc cggcgacatg cccagcggct gcggcccact ggcggcatcg
      301 atctcgatca tggcggtgcc tccttggctt cgcggtccag gcgcttctgc aggttctgca
      361 aaccggtggt ggccagcgcc cggcacgcgc ccgcgctgcc catcggttgc tcgccgcgca
      421 ggcgcggcca caggtcgctg acggacggat gcggggtcag caggatgtcg cagggctcga
      481 tgccggcaag cgcctgcatc ccggccttga aagccgtgcc gtagtcggga tggtcgcgat
      541 acaggtattc gccatccgcc agtgcgctca cgctgtcggc atagacgacc tgcctgcaat
      601 cggtgcctgc gcagctgcgc cagctgaagc tggtgctgcc gggcgcatgc ccgggcgtgg
      661 catgcacctg cacggacagc ggacccacct gcacgtgcgc gtcatcggcg atggcgcgtg
      721 cgccgggaac cgccgggaac ggcttcagct caaggtgctg cggatcattg cgttcgctgc
      781 gcccgctgcg caggaccggc agcgcggctg cgcgcgacag cacctgcgcg ccactcagtt
      841 gctgcaagcg ggcgatgccg ccggcatggt cgaagtgttc gtgcgtattg aggatgacgc
      901 gcacgtcgcg catgcgatgc ccgctggcct cgatgctgcg cgccacgccg ccggcggcgg
      961 cctcggtggc accgtcgatc aggatgtgtc ccgcgggcga ggtgatcagg atcgcgctga
     1021 tcccgcagct gcccacgtac caggtattgg cgaacacgtg gcgcggcgcg gtcgcctcgt
     1081 cccagtcgcc ttcgtccttg cagggcgcga ccacgggcac cgcggtcgcg acaggctgaa
     1141 tggcttcgac gcccggcgcc ggcgcggcgc atgcaaccaa cagcagtgtc gccacgcacg
     1201 aacgaggggc actcacctca gatctcccgc gccaaccgct cggcaaggcc ggtgtagctg
     1261 cccggcgtca tcgccagcag ccgctgcctg tcgccggcgg gcaggtccag gccggcgatg
     1321 aattcgcgca tcgaggcctc ggtgatgccg tggccacggg tcagcgcctt cagctgttcg
     1381 tacgggttcg gcaggccgtg gcggcgcatc accgtctgca cggcttcggc cagcacttcc
     1441 caggcggcat caagatcggc cgccaggcgg tccggattca cgctgagctt gtccaggccg
     1501 cgcagcaggg catcgaaacc gatcagcatg tggccgaagg cggtaccgag cgcacgcagc
     1561 acggtggaat cggtgaggtc gcgctgccag cggctgaccg gcagcttgat cgcgaaatgc
     1621 tcgaacagcg cgctggcgat gccgaaattg ccttccgcgt tctcgaagtc gatcgggttg
     1681 accttgtgcg gcatggtgct gc
//
LOCUS       DAORUK010000263         1690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_120788_length_1690_cov_21.697859, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000263 DAORUK010000000
VERSION     DAORUK010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120788_length_1690_cov_21.697859"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1690)
                     /locus_tag="PK818_11110"
     CDS             complement(<1..>1690)
                     /locus_tag="PK818_11110"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HQY83316.1"
                     /translation="MSASKSSLASLTLGAIGVVYGDIGTSVLYTLKEVFGSGHVPFTT
                     DNVYGVLSIVFWTLTIIVSLKYVTLVLRADNHGEGGLVAMLALASQSVKHKAPALRKW
                     MLLIGIFGTCLFYGDGVITPAITVLSAVEGLDVVSPAFRKAVIPLTLLILLALFWVQK
                     RGTAGIGRFFGPIMVLWFIAIAALGVWHIASHPEILAALSPHYALGFIWRQPGTAFII
                     LGAVVLCVTGGEALYADMGHFGRRPIRVAWFSIVMPCLTLNYFGQGALLLSEPEAVKN
                     PFFHMAPDWLTLPLVGLATMAAVIASQALISGAYSVTKQVIQLGYLPRLQIQHTSTRD
                     TGQIYMPFVNWSLFVMIVLAVVLFRSSSNLAAAYGIAVTLDMTITTVLTFFVIRYGWG
                     YPLALCIAATGFFFVIDLAFFSSNLLKLAEGGWFPILIGGAVFILMVTWKDGRELLSR
                     SLRDGSIDLKSFLESVLLSPPTRVPGTAVFLSAEPGIVPNALMHNLKHNKVLHAQNLF
                     VTRRAHEVPWIGLDKRLEVQALGSDCWQVVVHHGFKNDPDLPKALELLAGRGIDL"
BASE COUNT          317 a          549 c          579 g          245 t
ORIGIN      
        1 aggtcgatgc cgcggccggc cagcagctcc agcgccttgg gcaggtcggg gtcgttcttg
       61 aagccgtggt gcaccaccac ctgccagcag tcgctgccca gcgcctgcac ctccagccgc
      121 ttgtccagcc cgatccaggg cacctcgtgc gcgcgccggg tgacgaacag gttctgcgcg
      181 tgcagcacct tgttgtgctt gaggttgtgc atcagcgcgt tgggcacgat gccaggctcg
      241 gcgctcagga acaccgcggt gccgggcacg cgcgtgggcg gactgaggag taccgattcg
      301 aggaaggact tcaggtcgat cgagccgtcg cgcaggctgc ggctgagcag ttcgcggccg
      361 tccttccagg tcaccatcag gatgaacacg gcgccgccaa tcaggatcgg gaaccagccg
      421 ccctcggcca gcttgagcag gttggagctg aagaacgcca ggtcgatgac gaagaagaag
      481 ccggtggccg cgatgcacag cgccagcggg tagccccagc cgtagcggat gacgaagaag
      541 gtcagcaccg tggtgatggt catgtccagc gtcaccgcga tgccataggc cgccgccagg
      601 ttggacgagg agcggaacag caccaccgcc agcacgatca tcacgaacag gctccagttg
      661 acgaagggca tgtagatctg gccggtgtcg cgcgtgctgg tgtgctggat ctgcaggcgc
      721 ggcaggtagc ccagctggat cacctgcttg gtgacgctgt aggcgccgct gatcagcgcc
      781 tgcgaggcga tcaccgccgc catcgtggcc agcccgacca gcggcagcgt cagccagtcg
      841 ggcgccatgt ggaagaacgg gttcttcacc gcctcgggct cggacagcag cagcgcgccc
      901 tggccgaagt agttcagcgt caggcagggc atgacgatgg aaaaccaggc cacgcggatc
      961 ggccgcctgc cgaaatggcc catgtcggcg tacagcgcct cgccgccggt gacgcacagc
     1021 accaccgcgc ccaggatgat gaaggcggtg cctggctggc gccagatgaa gcccagtgcg
     1081 tagtgcgggc tgagcgcggc caggatttcc gggtggctgg cgatgtgcca gacaccgagc
     1141 gccgcgatgg cgatgaacca cagcaccatg atcggcccga agaagcgccc gatgccggcc
     1201 gtgccgcgct tttgcaccca gaacagcgcc agcaggatca gcagcgtgag cgggatcacg
     1261 gccttgcgga acgcgggcga gaccacgtcc agcccctcca ccgccgacag caccgtgatg
     1321 gccggggtga tgacgccgtc gccgtagaac aggcaggtgc cgaagatgcc gatcagcagc
     1381 atccacttgc gcagcgccgg cgccttgtgc ttcaccgact gcgaggccag cgccagcatg
     1441 gccaccaggc cgccttcgcc atggttgtcg gcgcgcagca ccagggtcac gtatttcagc
     1501 gagacgatga tcgtcagcgt ccagaacacg atcgacagca cgccgtagac gttgtcggtc
     1561 gtgaacggca cgtggccgct gccgaacact tccttcagcg tgtacagcac gctggtgccg
     1621 atgtcgccat acaccacgcc gatggcgccc agcgtcaggc tggcgaggga agacttggaa
     1681 gccgacatgg
//
LOCUS       DAORUK010000264         1679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_122204_length_1679_cov_20.439655, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000264 DAORUK010000000
VERSION     DAORUK010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122204_length_1679_cov_20.439655"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..289
                     /locus_tag="PK818_11115"
     tRNA            214..289
                     /locus_tag="PK818_11115"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:247..249,aa:Trp,seq:cca)
     gene            338..721
                     /gene="secE"
                     /locus_tag="PK818_11120"
     CDS             338..721
                     /gene="secE"
                     /locus_tag="PK818_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019186114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HQY83317.1"
                     /translation="MTSKTLHDPSASAGDIAKYALALLLAVAGLVAFYWFDGQWPTGV
                     RVLVLVAGLAAGAFAFMATSKGAEAREFLAESRFELRKVVWPTRQETTRTMWVVIAVV
                     ILMSLMLAGMDLVIQAAVKWLLGNG"
     gene            732..1322
                     /gene="nusG"
                     /locus_tag="PK818_11125"
     CDS             732..1322
                     /gene="nusG"
                     /locus_tag="PK818_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HQY83318.1"
                     /translation="MTDSPEQGAQADKRWYVVHAYSGFEKSVAQALRDRIARMGMQER
                     FGEVLVPTEEVIEMRAGQKRRSERKFFPGYVLVQIATHDEAGIPRMDNESWHLVKETP
                     KVMGFIGGTADRPLPIRDTEADAILQRVQEGVEKPKPKVLFEPGQMVRVVDGPFNDFN
                     GVVEEINYDKSRLRVAVLIFGRSTPVELEFGQVEKA"
     gene            1538..>1679
                     /gene="rplK"
                     /locus_tag="PK818_11130"
     CDS             1538..>1679
                     /gene="rplK"
                     /locus_tag="PK818_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993334.1"
                     /note="binds directly to 23S ribosomal RNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HQY83319.1"
                     /translation="MAKKVVGYIKLQVKAGQANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATS"
BASE COUNT          304 a          531 c          588 g          256 t
ORIGIN      
        1 cgcgaaggcg gccgtaccgt cggcgccggc gtggtggcca agatcctcaa gtaatgcagg
       61 tgcgcgtccg cggcaacgcg gacgcgcacc gagcaatccc cgcgcaggcg gggaaccggt
      121 tccggcaagc cgccaagcgc gccgggacaa gggcaggcgg cgcaagccgc cgttgctttt
      181 tcggaagcga cggaattcca gatccaaatc gatacgccag tagctcaatt ggcagagcag
      241 cggtctccaa aaccgcaggt tgggggttcg agtccctcct ggcgtgccac ctgcgcaact
      301 cgcgtcgccg gcgcgtgcgc caaccgggat tgacgttttg accagcaaga ccctgcatga
      361 cccgtccgcc tccgccgggg acatcgcgaa gtacgccctg gcgctgctgc tggcggtcgc
      421 cggcctggtt gccttctatt ggttcgatgg ccagtggcca accggcgtgc gcgtgctcgt
      481 cctggtggcc gggctggcgg ccggcgcatt cgctttcatg gccaccagca agggcgccga
      541 agcccgcgag ttcctggcgg aatcgcgctt cgagctgcgc aaggtggtgt ggccgacgcg
      601 ccaggaaacg acgcgcacga tgtgggtggt gatcgcggtg gtcatcctga tgagcctgat
      661 gctcgccggc atggatctgg tgatccaggc ggcagtgaag tggttgctgg gcaacggttg
      721 aggatcgcaa gatgacggat agtccagaac agggtgcgca ggccgacaag cgctggtacg
      781 tggtgcacgc ctactccggc ttcgagaagt cggtggcgca ggcgctgcgc gaccgcatcg
      841 cgcggatggg catgcaggag cgcttcggcg aggtgctggt gcccaccgag gaagtgatcg
      901 agatgcgcgc cgggcagaag cgccgctccg agcgaaagtt cttcccgggc tacgtgctgg
      961 tccagatcgc gacccacgac gaagcgggca tcccgcgcat ggacaacgag tcctggcacc
     1021 tggtcaagga aacgccgaag gtgatgggct tcatcggcgg caccgccgac cgcccgctgc
     1081 cgatccgcga caccgaggcc gacgcgatcc tgcagcgcgt gcaggaaggc gtggagaagc
     1141 cgaagccgaa ggtgctgttc gagccgggcc agatggtccg cgtggtcgac ggcccgttca
     1201 acgacttcaa cggcgtggtc gaggagatca actacgacaa gagccgcctg cgggtggcgg
     1261 tgctgatctt cggtcgttcg accccggtcg agctcgagtt cggccaggtc gaaaaggcct
     1321 gacccgtcgg ggccggcccc tccgggcagg ctccgcaaaa acctgttatc ctttccggct
     1381 ccccgcgggg aaccagaaaa cgaccggccg cctcgtgcgg ccgtcggcac gaatgcaatg
     1441 cggtgaagtg acgccgggac gcgcgccgcg cttgaacgaa tcgatcccgg tccgatgggg
     1501 agcctgacaa caggcgcttg cacccggaga agcagcaatg gcaaagaaag tcgtcggtta
     1561 catcaaactg caggtcaagg ccggccaggc caatccgtcg ccgccggtgg gtcctgccct
     1621 cggccagcgc ggcctgaaca tcatggagtt ctgcaaggcg ttcaacgcag cgacctcca
//
LOCUS       DAORUK010000265         1562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_138406_length_1562_cov_19.599204, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000265 DAORUK010000000
VERSION     DAORUK010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138406_length_1562_cov_19.599204"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1082
                     /locus_tag="PK818_11135"
     CDS             198..1082
                     /locus_tag="PK818_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HQY83320.1"
                     /translation="MGIDDNRRDLEAGIHTDLAGRMTYGGYLQLPTLLSAQQPLSQPP
                     HHDEMLFIIQHQTSELWLKLLIHELTAAVGHLREDRVWQFGKVVARCKRVLDQLTAQW
                     SVLETLTPSEYMEFRAVLGPSSGFQSLQYRSVEFLMGNKNAAMLKVFAHDQAGHAALD
                     AVLRAPSLYDEFLRYLARWGHAVADAHLQRDWSQPHALDPALLPVFERIYEDTGLYWR
                     EYALCEDLVDLETQFQLWRFRHMRTVQRIIGFKPGTGGSSGVAFLRKALDLTFFPELF
                     EVRTAIGPGRADAAADAP"
     gene            1232..>1562
                     /locus_tag="PK818_11140"
     CDS             1232..>1562
                     /locus_tag="PK818_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQY83321.1"
                     /translation="MSGAATSTPGPIPEYTQLMGHPRPLWMLFMTEFWERFAFYGIRW
                     ALVLYIVAQFHGGADTGEASANQTYGAYLALVYAAAIFGGYVADKIIGYQRSILVGAI
                     IMAAGLFM"
BASE COUNT          238 a          527 c          526 g          271 t
ORIGIN      
        1 cctgcgcctg caccgcgaac ggcgcgcaca aggccagcgc caacagcgcg gcgcgctgga
       61 atagctgttt catcaagtgg attcccgggg ttgccgcgaa ccggcccccg gctcgcaccg
      121 cgtgattgtg ctgcattttc gtgacagttg cgtggcgtat ggaacggccc ggccgggtcg
      181 cgtgcgagaa tccggcgatg ggcatcgacg acaaccggcg cgacctggaa gcgggcatcc
      241 ataccgatct tgccggccgc atgacctatg gcggctacct gcagctgccg accctgctgt
      301 ccgcgcagca gccgctgtcg cagccgccgc accacgacga gatgctgttc atcatccagc
      361 accagaccag cgagctgtgg ctgaagctgc tgatccacga gctgaccgcc gcggttggcc
      421 acctgcgtga ggatcgggtc tggcaattcg gcaaggtggt ggcgcgctgc aagcgggtgc
      481 tggaccagct gaccgcgcag tggtcggtgc tggagacgct gacgccgtcc gagtacatgg
      541 agttccgcgc ggtgctcggg ccttcctccg gcttccagtc cctgcagtac cgcagcgtcg
      601 aattcctgat gggcaacaag aacgcggcga tgctcaaggt cttcgcccac gaccaggccg
      661 gccatgccgc gctggacgcg gtgttgcgcg cgcccagcct gtacgacgag ttcctgcgct
      721 acctggcgcg ctgggggcac gcggtggcgg atgcccacct gcagcgcgac tggtcgcagc
      781 cgcacgcgct ggacccggcg ctgctgccgg tgttcgagcg catctacgag gacaccggcc
      841 tgtactggcg cgaatacgcc ctgtgcgagg acctggtcga cctcgagacc cagttccagc
      901 tgtggcggtt ccggcacatg cgcacggtgc agcggatcat cggcttcaag cccggcaccg
      961 gcggttccag tggggtggcg ttcctgcgca aggcgctgga cctgaccttc ttccccgagc
     1021 tgttcgaggt gcgcaccgcg atcggcccgg gtcgcgccga tgcggcggcc gatgccccct
     1081 gaacccgcgt cgctgctgca tcgcagcagc ctggcctgaa tgcgcgaggt ttgacgtggg
     1141 atcgccggtt cggtgatgct ccgccggttc cgcgcacgcc gcggaccgac attccgactt
     1201 ctttccacga atcaaagacg ggggaaaccg catgagcggt gccgcaactt cgacgccggg
     1261 cccgatcccg gaatacaccc agttgatggg tcatccgcgt ccgctgtgga tgctgttcat
     1321 gaccgaattc tgggagcgtt tcgcgttcta cgggatccgc tgggcgctgg tgctgtacat
     1381 cgtcgcccag ttccacggcg gcgccgacac cggcgaggcc tcggccaacc agacctacgg
     1441 cgcctacctg gcgctggtgt acgcggcggc gatcttcggc ggttacgtgg ccgacaagat
     1501 catcgggtac cagcgctcga tcctggtcgg cgcgatcatc atggccgccg gcctgttcat
     1561 ga
//
LOCUS       DAORUK010000266         1551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_140092_length_1551_cov_18.518048, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000266 DAORUK010000000
VERSION     DAORUK010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140092_length_1551_cov_18.518048"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1047)
                     /locus_tag="PK818_11145"
     CDS             complement(1..1047)
                     /locus_tag="PK818_11145"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HQY83322.1"
                     /translation="MTTKAYGAHAADQPVVALDIQRRAPGPGDVQIDIAYCGVCHSDL
                     HTVRSEWPGTLYPCVPGHEIVGHVSAVGSDVSGFKVGDTVGVGCLVDSCRHCASCSEG
                     LEQYCTNGFVGTYNGPTADAPGHTLGGYAQRIVVEQRFVLKVRHPEDQLAAVAPLLCA
                     GITTYSPLRHWQAGPGKKVGIVGIGGLGHMGIKIAHAMGAHVVAFTTSESKRDEAHGL
                     GADEVVISKDAAQMKAHAGSFDFILNTVAAPHVLDAFTALLKRDGTLCLVGAPASPHP
                     SPSVMGLIFGRKAIAGSLIGGIPETQEMLDFCAEHGIVADIEMIAAGDIEAAYERMLR
                     SDVKYRFVIDTASM"
     gene            complement(1124..>1551)
                     /locus_tag="PK818_11150"
     CDS             complement(1124..>1551)
                     /locus_tag="PK818_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HQY83323.1"
                     /translation="GVGRQLDPKIDIWAVAKPVLEQILVERYSPQRLAGEFRKRLPEM
                     VTRAPEVPRLLHAWLQQQVEGKHELSMRSRDLAELARIAHAAQKRVVAAVLGSGLLIV
                     AAVLYALEAGGPQLLGVPVSTWVAGLGGAWALLAAWPRR"
BASE COUNT          238 a          557 c          522 g          234 t
ORIGIN      
        1 ttacatcgac gccgtgtcga tcacgaagcg gtacttcacg tcgctcctga gcatgcgctc
       61 gtaggccgcc tcgatgtcgc ccgcggcgat catctcgatg tcggccacga tgccgtgctc
      121 ggcgcagaaa tccagcatct cctgggtttc cgggatgccg ccgatcagcg aaccggcgat
      181 cgccttgcgg ccgaagatca ggcccatcac gctggggctg ggatgcggcg acgccggcgc
      241 gccgaccagg cacagggtgc cgtcgcgctt gagcagcgcg gtgaacgcat ccagcacgtg
      301 cggcgcggcc acggtgttga ggatgaagtc gaagctgccg gcatgcgcct tcatctgcgc
      361 ggcatccttc gagatcacca cctcgtcggc acccaggcca tgcgcttcat cgcgcttgga
      421 ttcgctggtg gtgaacgcga ccacgtgcgc gcccatcgca tgcgcgatct tgatgcccat
      481 gtggccaagc ccgccgatgc cgacgatgcc gaccttcttg cccggcccgg cttgccagtg
      541 gcgcagcggc gaataggtgg tgatgccggc gcacagcagc ggcgccaccg cggccagctg
      601 gtcttccgga tggcggacct tcagcacgaa gcgctgctcc accacgatcc gctgcgcata
      661 accgcccagc gtgtggcccg gggcgtccgc ggtcggaccg ttgtaggtgc cgacgaagcc
      721 gttcgtgcag tactgctcca gcccttcgct gcaggacgcg cagtggcggc agctgtcgac
      781 caggcaaccc acgccgacgg tatcgccgac cttgaagccg ctgacgtcac tgccgaccgc
      841 gctgacatgg ccgacgatct cgtggcccgg cacgcacgga tacagggtgc ccggccattc
      901 cgaacgcacg gtgtgcaggt cggaatggca gacgccgcaa taggcgatgt cgatctgcac
      961 gtcgcccggg ccgggcgcgc ggcgctggat gtcgagggcg acgacgggct ggtcggcggc
     1021 gtgggcgcca taggctttgg tggtcatgcg gactccgcgg tgatcgggga gcccgcacca
     1081 tactccgacc ggattgcagc ggcagcatcg ccgctggaac gcatcagcgc cgtggccacg
     1141 ccgccagcag ggcccacgca ccgcccaggc ctgcgaccca ggtggacacc ggcacgccaa
     1201 gcagctgcgg cccgcccgcc tccagcgcgt acagcacggc ggccacgatc agcaggccgg
     1261 atccgagcac cgccgcgacc acccgcttct gcgcggcatg cgcgatgcgc gccagttcgg
     1321 cgaggtcgcg cgagcgcatc gacagctcgt gcttgccctc gacctgctgc tgcagccacg
     1381 cgtgcagcag gcgcggcact tcgggcgcgc gcgtgaccat ttccggcagc cgcttgcgga
     1441 actcgccggc cagccgctgc gggctgtagc gttcgaccag gatctgctcc agcaccggct
     1501 tggccaccgc ccagatgtcg atcttcggat ccagctggcg gcccacgccc t
//
LOCUS       DAORUK010000267         1539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_141969_length_1539_cov_17.084232, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000267 DAORUK010000000
VERSION     DAORUK010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141969_length_1539_cov_17.084232"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1510)
                     /locus_tag="PK818_11155"
     CDS             complement(<1..1510)
                     /locus_tag="PK818_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQY83324.1"
                     /translation="MATSRNDDVLVIGGGHNGLVCAAYLAAAGLKVRVLERRHLVGGA
                     AVTEEFHPGFRNSLASYTVSLLNPKVIADLRLAEHGLRVVERSYANFLPLPDGRSFRL
                     GGEHTHSEVAKWSARDAQRLPDYYAMLDRIVVVLRALMQRTPPNVSDRFVLADWLNSA
                     SAAKQLKTLDMRGRRDLLDLFTKSAGELLDRWFEGEPLKAALGWDSVVGNFASPYTPG
                     SAYVLLHHVFGEVNGKGGAWGHAIGGMGAITQAMRAECEARGVAIETDAEVARLLVEN
                     GKAVGVALADGRELRAATIASNLNPKLLYTKLVERNQLDEDTATRIEGYRCGSGTFRM
                     NVALSELPDFTAMPGTRLQPHHRSGILIGPSLQYFEQAYFDAKSRAHNPGWARQPIVE
                     VVISSTLDDTLAPAGQHVASLFCQQVNPVIADADGGWDAHRDTVARLMIDTVDAHAPN
                     FARSVLGYEALSPLDLERRIGLVGGDIFHGALGLDQMFSARPLLGQGNYRGAF"
BASE COUNT          251 a          540 c          511 g          237 t
ORIGIN      
        1 tgaacgcgcc gcgatagttg ccctgcccca gcagcgggcg cgcgctgaac atctggtcca
       61 gcccgagtgc gccgtggaag atgtcgccgc cgaccaggcc gatccggcgc tccaggtcca
      121 gcgggctcaa tgcctcgtat cccagcacgc tgcgggcgaa gttcggcgca tgcgcatcga
      181 cggtatcgat catcagcctg gcaaccgtgt cgcggtgcgc gtcccagccg ccgtccgcgt
      241 cggcgatcac gggattcacc tgctggcaga acaggctggc cacatgctgg ccggcgggcg
      301 ccagggtgtc gtcgagcgtc gaggaaatca ccacctcgac aatcggctgg cgcgcccagc
      361 cggggttgtg tgcacgcgac ttggcatcga aataggcctg ctcgaaatac tgcagcgacg
      421 gcccgatcag gatcccgctg cggtggtgcg gctgcagccg ggtgccgggc atcgcggtga
      481 aatccggcag ctccgacagc gccacgttca tgcggaaggt gccggagccg cagcggtagc
      541 cctcgatgcg ggtggcggtg tcttcgtcca gctggttgcg ctcgaccagc ttcgtgtaca
      601 gcaatttcgg gttgaggttg ctggcgatgg tcgccgcgcg cagttcgcgg ccatcggcga
      661 gggcgacgcc caccgccttg ccgttctcca ccaggagccg ggcgacttcg gcatcggtct
      721 cgatggcgac gccacgcgcc tcgcattcgg cgcgcatcgc ctgggtgatc gcgcccatcc
      781 cgccgatcgc gtggccccac gcgccgccct tgccgttgac ctcgccgaac acgtggtgca
      841 acagcacata cgcactcccc ggcgtgtacg ggctggcgaa attgccgacc accgaatccc
      901 agcccagcgc ggccttcagc ggttcgccct cgaaccagcg atccagcagc tcgccggcgg
      961 acttggtgaa caggtcgagc aggtcgcggc ggccgcgcat gtccagcgtc ttcaactgct
     1021 tcgccgccga tgccgaattg agccagtcgg ccagcacgaa gcggtcggaa acattcggcg
     1081 gcgtgcgctg catcagcgcg cgcagcacca ccacgatccg gtccagcatc gcgtagtagt
     1141 ccggcaagcg ctgggcatcg cgcgccgacc acttcgccac ctccgaatgc gtgtgctcgc
     1201 cgccgagcct gaagctgcgg ccatcgggca aaggcaggaa gttcgcatag gaccgttcca
     1261 ccacgcgcaa gccatgctcg gcaaggcgca ggtcggcgat caccttcggg ttgagcaggc
     1321 tgaccgtgta gctggccagc gagttgcgga agcccggatg gaattcctcg gtcaccgccg
     1381 cgccgccaac caggtggcgg cgctccagca cccgcacctt cagcccggct gcggccaggt
     1441 aggccgcgca gaccaggccg ttgtggccgc caccgatgac cagtacatcg tcgttccggg
     1501 aagtcgccac gcgcggcacc gattcgggaa acagccgat
//
LOCUS       DAORUK010000268         1534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_142782_length_1534_cov_17.045977, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000268 DAORUK010000000
VERSION     DAORUK010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142782_length_1534_cov_17.045977"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1383
                     /locus_tag="PK818_11160"
     CDS             136..1383
                     /locus_tag="PK818_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HQY83325.1"
                     /translation="MNAPSELRDGTLTPSAMLALLAAAPHRLLFFAGASGVLLSMAWW
                     AAWLVGVRWSALALPPPPVPAGWAHAIGMQYQVLGLFIFGFLLTVFPRWMDQPAFGRR
                     HYVPVAGAIFAGYLLTLASLLGFPLLLHAGVVLTLLGWTVGCLLLLRVLWRDGGSQYH
                     ALSIWAALALGGVGLAMFARYLHSGDARLAFGAIKIGTFGFLLPVYATVAHRMVPFFA
                     QAAIPGRAMYRPGWILASFWVLVLAHLMGELAHAYAWLWPVDLALAVLTGYWWWRWQP
                     LRTRGIRLLWVLHLAFAWLPVAFGLYALQGAIYAATGAFELGRAPVHALTVGYFGSML
                     VAMVTRVTQGHSGRPLEMGRVAWACFLGVQAVSVLRIAAELLPDSPAWMAVAAVGWLL
                     AFLPWVLRSLGIYLSPRADGKPG"
BASE COUNT          154 a          538 c          554 g          288 t
ORIGIN      
        1 gccggtgcag aattcgctgc atgccgacgc gttcggcagt ttgatccagg tgcccgccgg
       61 ccggcgtctc cccacgccgg ccgacatgca gcgggtcccg ccctgcgcgg ggcccgcctc
      121 caacacccga atcccatgaa cgccccatcc gaactccgcg acggcaccct gacaccgtcc
      181 gccatgctgg cgctgctcgc cgccgcgccg caccgcctgc tgttcttcgc cggcgccagc
      241 ggcgtgctgc tgtcgatggc gtggtgggcc gcgtggctgg tcggcgtgcg ctggtcggcg
      301 ctggcgctgc cgccgccccc ggttccggcc gggtgggccc acgccatcgg catgcagtac
      361 caggtgctgg ggctgttcat cttcggattc ctgctcacgg tattcccgcg ctggatggac
      421 caaccggcat tcgggcgacg ccactacgtc ccggtcgccg gcgcgatctt cgccgggtac
      481 ctgctcacgc tcgcgagcct gttgggattc ccgctgctgc tgcatgccgg cgtggtgctg
      541 acgctgctgg ggtggacggt cggctgcctg ctgctgctgc gcgtgctgtg gcgcgacggc
      601 ggcagccagt accacgcgct ctcgatctgg gccgcgctgg cgctgggcgg cgtgggcctg
      661 gcgatgttcg cgcgctacct gcactcgggc gatgcgcggc tggccttcgg cgcgatcaag
      721 atcgggacct ttggcttcct gctgccggtc tacgccacgg tggcgcaccg catggtgccc
      781 ttcttcgcgc aggccgcgat tccgggccgc gcgatgtacc ggcccggctg gatcctcgcg
      841 agcttctggg tcctggtgct cgcccacctg atgggcgaac tcgcgcacgc ctacgcgtgg
      901 ctgtggccgg tcgacctggc actggcggtg ctcacgggct actggtggtg gcgctggcaa
      961 cccttgcgga cgcgcggcat ccgcctgctg tgggtgctgc acctggcctt cgcgtggctg
     1021 ccggttgcgt tcgggcttta cgcgctgcag ggcgcgatct acgccgcgac gggcgccttc
     1081 gaactcggac gcgcgcccgt gcacgcgctg acggtgggct atttcggcag catgctggtg
     1141 gcgatggtca cgcgggtgac gcagggccac tcgggccggc cgctggagat gggccgcgtc
     1201 gcctgggcct gcttcctcgg cgtgcaggcg gtgagcgtgc tgcgcatcgc cgccgaactg
     1261 ctgccggatt cgccggcatg gatggccgtg gcagcggtgg gttggctgct cgccttcctg
     1321 ccgtgggtgc tgcgttcgct ggggatctac ctcagcccgc gcgcggatgg caagccgggg
     1381 tgatcgagta ctacgcgcag gtgaaatggc tgcacgtggg gacggtgatc ctctccggtg
     1441 cactgttctg cgctcggggc ctgatgatgc tggcgcgctc gccacgggcc aaccatgcgc
     1501 tgctgcgctt tccttcctac gtcatcgaca ccgc
//
LOCUS       DAORUK010000269         1505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Thermomonas sp. isolate AMR_MDS_5267
            NODE_147786_length_1505_cov_16.462759, whole genome shotgun
            sequence.
ACCESSION   DAORUK010000269 DAORUK010000000
VERSION     DAORUK010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867484
            Sequence Read Archive: SRR5580759
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomonas sp. (wastewater metagenome)
  ORGANISM  Thermomonas sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Thermomonas.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 20:44:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,234
            CDSs (total)                      :: 2,203
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 3 of 24
            Pseudo Genes (incomplete)         :: 22 of 24
            Pseudo Genes (internal stop)      :: 0 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Thermomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147786_length_1505_cov_16.462759"
                     /isolate="AMR_MDS_5267"
                     /isolation_source="Active sludge of wastewater treatment
                     plant"
                     /db_xref="taxon:1971895"
                     /environmental_sample
                     /geo_loc_name="Austria"
                     /lat_lon="48.3 N 16.2 E"
                     /collection_date="2014-01-28"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..713
                     /locus_tag="PK818_11165"
     CDS             186..713
                     /locus_tag="PK818_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017159325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQY83326.1"
                     /translation="MTEATIRRATPEDAPTLAELGAATFIEAFGQLYTPADLQAFLAE
                     SHSPAGYAKALANPDYALWIAERDGQAIGYAQAGPCGLPHADASPADGELKRLYLLKS
                     AQNGGVGGALFEQALAWLERDGPRTLWISVWSENYGAQRFYGRHGFAFAGEYAFIVGE
                     QRDREFMYRRAPRPA"
     gene            710..1327
                     /locus_tag="PK818_11170"
     CDS             710..1327
                     /locus_tag="PK818_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005996584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQY83327.1"
                     /translation="MTGKVLLVHGLLNATSWLRPLAARLRAHGFAPELFGYSSLLDGP
                     QRAVPRLVERLRREPVDAMVGHSLGGLIALEALRAEPGLAVPRVVCIGTPLRGSASAR
                     GLAARGWTRPLLGRSAGLLQQGLQRWDGAAAVGVVAGDVARGMGRLFCRFDGASDGTV
                     GLEETRLPGIADHCTVHCSHTGLVFSADAAAQAAHFLRHGRFAQA"
BASE COUNT          194 a          527 c          560 g          224 t
ORIGIN      
        1 ggggtgctgc tacccgacgc gtgcgaacat cctgttcgac gcgtcgccgc aggcccgctc
       61 cgcggcccgg cccggcgcgg agcaccgggc gttcgcgcgc ttgcaccgca tgcggtacaa
      121 gcaaggcgcg ctcacccatg gcctgctttc cgcagcaccc cggcagcgcc catcgcggag
      181 gtgtcatgac agaagcaacg atccgccgcg ccaccccgga ggacgcgccc acgctggccg
      241 agctcggcgc ggcgaccttc atcgaggcct tcggccagct gtacacgccg gccgacctgc
      301 aggctttcct cgccgagagc cattcccctg cggggtatgc gaaggcgctg gcgaatcccg
      361 actacgcgct gtggatcgcc gagcgcgacg gccaggcgat cggctacgcg caggccggtc
      421 cgtgcgggtt gccgcatgcg gacgcgagcc ctgccgatgg cgaactgaag cggctgtacc
      481 tgctgaagtc cgcgcagaac ggtggcgttg gtggcgcgct gttcgagcag gcgctggcgt
      541 ggctggaacg cgacggcccg cgcacgctgt ggatcagcgt gtggtcggag aattacggcg
      601 cgcagcgctt ctacgggcgg catggcttcg cgttcgcggg cgaatacgcc ttcatcgtcg
      661 gcgaacagcg cgaccgcgaa ttcatgtacc ggcgcgcgcc ccggccggca tgaccgggaa
      721 ggtgctgctg gtccacggcc tgctgaacgc caccagctgg ttgcggccgc tggccgcgcg
      781 cctgcgcgcg cacgggttcg cgccggaact gttcggctat tccagcctgc tcgatggccc
      841 gcagcgcgcg gtgccgcggc tggtggagcg cctgcgtcgc gaaccggtgg atgcgatggt
      901 cgggcacagc ctgggtggcc tgatcgcgct cgaagccctg cgcgccgagc ccgggctggc
      961 ggtgccgcgg gtggtctgca tcggcacccc gctgcgcggc agcgccagcg cgcgcgggct
     1021 ggcggcgcgt ggctggacca ggccgttgct gggccgcagc gcgggcctgc tgcagcaggg
     1081 cctgcagcgc tgggacggag cggccgcggt gggggtggtg gcgggcgacg tggcgcgcgg
     1141 catgggccgg ctgttctgcc ggttcgatgg cgcctccgat ggcaccgtgg ggctggagga
     1201 aacccggctg ccgggcattg ccgaccattg cacggtccac tgcagccata ccggcctcgt
     1261 gttttcggcg gatgccgcgg cgcaggccgc gcatttcctg cgccacggtc gtttcgcgca
     1321 ggcctgaccg gcaccgcgca cgccgcccgc atcaccgccg cggtggcctc cgcccgcggc
     1381 ttcggccggc gcaaggccgt ataattcgcg accttcggtt ccgctggatg atgcaatggg
     1441 acgtggtcct tccatcgaag gtcgcaagaa cgcggtcgac gccaagcgcg gcaagatctt
     1501 cacca
//
